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limit use dithionit quench determin topolog membran insert protein determin topolog membran insert protein peptid often reli upon indirect fluoresc measur one techniqu use nbd environment sensit fluorophor coval link protein relat hydrophil environ nbd hydrophob environ show increas emiss intens shift shorter wavelength gain insight nbd fluoresc chemic quench use dithionit dithionit anion expect penetr outer leaflet interfaci region exclud hydrocarbon core inner leaflet lumen luv assumpt hold neutral ph larg number nbddithionit experi carri report control experi luv direct label nbd pe assess dithionit quench acid condit result show acid ph dithionit move freeli across bilay quench inner leaflet buffer condit use dithionit exhibit sharp chang behavior ph therefor acid condit dithionit could differenti leaflet nbd resid

D005456;D008565

T130;T192;T116;T123

isol post traumat astereognosi case base review astereognosi tactil inabl recogn object place palm touch eye close blind fold presenc intact primari sensori modal stereognosi usual consid function contralater sensori cerebr cortex howev lesion sever anatom area patholog entiti report associ astereognosi two previous report link traumat injuri isol astereognosi follow surgic evacu traumat pariet extradur hematoma follow bullet injuri neck respect

D006225;D013236

T039;T023

loss chromatin remodel ddm caus segreg distort arabidopsi thaliana ddm mutant dna methyl primarili affect heterochromat region chromosom associ segreg distort snps f progeni segreg distort sd common genet map experi valuabl resourc determin gene loci induc deviat meiotic dna cross sd control sever type epigenet modif dna methyl import regulatori epigenet modif inherit across generat present studi investig relationship sd dna methyl ecotyp col c chromatin remodel mutant ddm col ddm c reciproc cross obtain f generat total plant reciproc cross plant f generat subject next generat sequenc detect singl nucleotid polymorph snps dna marker snps analyz use chi squar test method determin segreg ratio f generat segreg ratio whole genom snps classifi class class snps reciproc cross wild type show expect mendelian ratio reciproc cross ddm mutant show distort contrast snps class display normal ratio class show sd regardless wild type mutant assess use cap cleav amplifi polymorph sequenc marker analysi confirm next generat sequenc ddm mutant dna methyl high reduc throughout whole genom signific heterochromat region chromosom result show ddm mutant exhibit low level dna methyl facilit sd snps primarili locat heterochromat region chromosom reduc heterozyg ratio present studi provid strong base futur research focus impact dna methyl trait segreg plant evolut

D017360;D029681

T116;T002

identif novel genom wide pleiotrop associ oral inflammatori trait oral inflammatori diseas oid group dental diseas multipl clinic manifest impact major world popul mani studi investig associ individu oid trait genom variant whether pleiotrop loci share oral inflammatori trait remain poor understood conduct multitrait joint analys base summari statist genom wide associ studi gwass five dental trait uk biobank among genom wide signific loci two novel pain gum toothach identifi causal variant novel locus function annot base multiom data suggest il ilatrim potenti candid gene novel pleiotrop loci subsequ analys pathway enrich protein protein interact network suggest involv candid gene immun regul conclus result uncov novel pleiotrop loci oid trait highlight import immun regul pathogenesi oid find enhanc understand pathogenesi oid benefici risk screen prevent develop novel drug target immun regul oid

D058685

T028;T045

rare frequent lethal complic pulmonari vein isol year old patient perceiv dysphagia day uncompl pulmonari vein isol symptom complaint fever malais despit prophylact treatment sever complic evolv could detect time close control allow earli treatment

D001281;D017115;D004937;D011667

T023;T047;T046;T190;T061

real world experi wearabl cardiovert defibril clinic effect wear time adher patient high risk sudden cardiac death previous studi establish role wearabl cardiovert defibril wcd effect safe bridg temporari risk sudden cardiac death scd patient advanc heart failur prognost relev wcd remain controversi

D047548;D000076251

T074;T073

nausea dizzi patient heart failur cardiac resynchron therapi year old man ischem cardiomyopathi sever reduc left ventricular function biventricular defibril primari prevent receiv cathet ablat multipl ventricular tachycardia despit amiodaron treatment week later patient present unspecif symptom general malais nausea

D058406;D017115;D017147;D006333;D017180

T047;T074;T061;T046

micropuls transscler cyclophotocoagul taiwanes popul year clinic outcom prognost factor evalu year efficaci safeti micropuls transscler cyclophotocoagul mp tscpc taiwanes patient glaucoma

D017075;D054023

T074;T073;T061

prior endoscop cyclophotocoagul ecp affect subsequ trabeculectomi outcom report long term outcom trabeculectomi follow prior endoscop cyclophotocoagul ecp

D005901;D014130

T047;T061

outcom combin augment micropuls limit continu wave cyclophotocoagul patient refractori glaucoma assess safeti effect augment micropuls mp tscpc limit continu wave transscler cyclophotocoagul cw tscpc patient refractori glaucoma

D005901;D015814

T047

cirs standard practic hepat transarteri chemoembolis cirs standard practic document aim intervent radiologist provid best practic perform transarteri chemoembolis develop expert write group guidanc cirs standard practic committe encompass technic detail reflect european practic differ tace procedur lp tace dem tace dsm tace b tace well revis exist literatur various clinic indic hcc mcrc icc net final new frontier develop also discuss

D006528;D016461;D008113

T191;T061

autofluoresc imag within liver promis tool detect character primari liver tumor assess perform nm induc autofluoresc character primari liver nodul ex vivo resect specimen

D001650;D006528;D008113

T191

digit breast tomosynthesi dbt plus synthesis two dimension mammographi sd breast cancer screen associ higher cancer detect lower recal compar digit mammographi dm alon result systemat review meta analysi digit breast tomosynthesi dbt plus digit mammographi dm screen problemat due increas radiat doubl exposur synthesis two dimension mammographi sd calcul dbt dataset addit dose appear sensibl altern compar ad dm systemat review meta analysi focus screen perform outcom women screen dbt plus sd compar dm alon

D001943;D055088

T058;T191;T060

diagnosi loosen knee arthroplasti asept loosen one common reason revis knee arthroplasti pathogenesi multifactori earli diagnosi necessari initi appropri therapi avoid serious complic substanti bone loss even periprosthet fractur

D019645;D007720

T074;T061

pattern syntroph interact methanogen convers propion methanogenesi central anaerob digest process convers propion key intermedi methanogenesi requir syntroph interact bacteri archaeal partner studi seri methanogen enrich propion sole substrat develop identifi microbi popul specif involv syntroph propion convers rigor control propion enrich exhibit function stabil consist propion convers methan product yet methanogen microbi communiti experienc substanti tempor dynam import implic understand mechan involv microbi communiti assembl anaerob digest syntrophobact identifi abund consist bacteri partner syntroph propion convers regardless origin sourc cultur concentr propion tempor dynam cultur contrast methanogen partner involv syntroph propion convers lack consist domin methanogen vari function process condit tempor dynam methanoculleus popul specif enrich syntroph partner inhibitori level propion like due abil function unfavor environment condit syntroph propion convers carri exclus via transform propion acet hydrogen enrich establish studi microbi popul high toler elev propion repres syntrophobact methanoculleus great signific understand methanogen activ process perturb propion accumul frequent encount key point syntrophobact consist bacteri partner propion metabol divers hydrogenotroph methanogen popul could serv syntroph partner methanoculleus emerg methanogen partner toler elev propion

D019605;D011422

T194;T121;T109

explor divers bacteriophag specif oenococcus oeni lactobacillus spp role wine product widespread exist bacteriophag great interest biolog research communiti ongo investig continu explor divers role also attract attent depth research connect ferment food process particular dairi wine industri bacteriophag most oenophag may fact doubl edg sword winemak whilst implic causal agent difficulti malolact ferment although proven also begin consid altern use sulphur dioxid prevent wine spoilag investig characteris oenophag oenococcus oeni main speci use winemak still limit compar lactococc bacteriophag lactococcus lacti lactiplantibacillus plantarum formal lactobacillus plantarum driver ferment dairi product interest strain also use consid use winemak review genet divers life cycl phage togeth debat consequ impact phage predat wine potenti control strategi discuss key point bacteriophag detect wine divers mani lysogen bacteriophag found wine bacteria phage impact winemak depend stage winemak process bacteriophag potenti antimicrobi agent spoilag organ

D001435;D056585;D014920

T007;T168;T005

behavior uptak ibuprofen five varieti horticultur crop irrig regener water current use regener water agricultur led emerg new form pollut pharmaceut compound pcs fulli elimin wastewat treatment plant wwtps therefor effluent wwtps use agricultur irrig presenc pcs must analys concentr determin main object studi evalu uptak ibuprofen ibp horticultur crop irrig wwtp effluent subsequ effect human health due incorpor food chain studi involv five varieti crop lettuc parsley cabbag zucchini broccoli grown greenhous irrig wwtp effluent water ibp analys varieti regener water irrig horticultur crop leav mini romain lettuc present detect level ibp without mean risk human health

D062065;D014874

T131;T069;T083

select recoveri estrogen endocrin disruptor environment sampl use substrat activ specif concentr mip molecular imprint polym mip shown select collect human estrogen receptor bind activ substanc howev environment sampl contain various chemic depend locat region differ bind activ depend sampl type thus general applic mip actual environment sampl must elucid studi environment sampl collect screen mip yeast assay perform evalu adsorpt characterist sampl accord adsorpt elut fraction result show mip collect bind activ substanc almost select collect constitut androstan receptor car bind activ substanc select car bind activ detect adsorb fraction sever bind activ substanc also demonstr car bind activ

D052244;D014874

T131;T039

dabigatran plasma concentr indic risk patient non valvular atrial fibril studi aim evalu variabl dabigatran plasma concentr associ clinic event chines patient treat dabigatran etexil de non valvular atrial fibril nvaf steadi state concentr dabigatran activ metabolit de determin trough peak effect dabigatran concentr variabl relat factor clinic outcom explor data patient receiv fix dose mg show dabigatran trough concentr vari remark age bmi histori heart failur identifi import covari dabigatran trough concentr dabigatran trough concentr p histori hypertens p score identifi key factor predict risk bleed event dabigatran trough concentr affect age bmi histori heart failur may serv independ risk factor bleed event chines patient treat de nvaf

D001281;D006333

T047;T046

ipa improv rice drought toler seedl stage main activ abscis acid pathway key messag ipa enhanc rice drought toler main activ aba pathway endow rice seedl develop root system smaller leaf stomata apertur enhanc carbon metabol drought major abiot stress crop product ipa ideal plant architectur osspl encod transcript factor report function rice ideal plant architectur biotic resist pair ipa ipa nil near iso genic line found ipa could signific improv rice drought toler seedl stage ipa plant better develop root system smaller leaf stomat apertur analysi carbon nitrogen metabol associ enzym activ gene express metabol profil indic ipa could tip carbon nitrogen metabol balanc toward increas carbon metabol pattern control peg treat condit aba content ipa seedl signific higher ipa seedl express aba biosynthesi gene detect regul wherea express aba catabol gene regul ipa seedl addit base yeast one hybrid assay dual luciferas assay ipa found direct activ promot activ oshox transcript factor promot aba biosynthesi osnac posit regul aba pathway express oshox osnac signific regul ipa plant combin previous studi result suggest ipa could improv rice seedl drought toler main activ aba pathway regul ipa mediat aba pathway import strategi improv drought resist rice

D055864

T070

racial differ outcom ibd hospit nation popul base studi scarc data describ outcom hospit patient admit inflammatori bowel diseas ibd stratifi race retrospect cohort studi evalu differ outcom adult white black patient hospit princip diagnosi inflammatori bowel diseas

D015212

T047

lymphocyt subset peripher blood disturb system sclerosi patient chang immunosuppress medic system sclerosi ssc sever chronic diseas broad spectrum clinic manifest ssc display disturb lymphocyt homeostasi immunosuppress medic target b cell improv diseas manifest ssc clinic manifest immunosuppress medic caus chang lymphocyt subset aim studi investig peripher lymphocyt homeostasi ssc regard immunosuppress major organ involv ssc patient healthi donor hd includ immunophenotyp peripher whole blood fluoresc activ cell sort perform cytokin secret stimul b cell cultur measur ssc patient without immunosuppress compar hd display lower cell lower helper cell cd cd lower transit b cell cd cd cd igd lower pre switch memori b cell cd cd igd lower post switch memori b cell cd cd igd differ cytokin product whole b cell cultur ssc hd within ssc cohort mycophenol intak associ lower helper cell lower nk cell cd cd describ differ peripher lymphocyt subset ssc hd generat insight ssc pathogenesi lymphocyt chang effect immunosuppress indic lymphocyt homeostasi ssc might restor

D007166;D016131;D012595

T129;T047;T121;T185

permeabl lipid bilay low increas membran cholesterol oxygen transport rout lung tissu mitochondria cross sever cell membran permeabl valu membran p although fundament import controversi previous studi most indirect method diverg cms use direct approach observ transmembran flux nm liposom defin transmembran gradient stop flow system due small size liposom intra well extraliposom diffus process affect overal kinet releas process find cholesterol free liposom unexpect low p valu cms c p would present serious obstacl enter leav erythrocyt cholesterol turn novel major modifi p abl increas p order magnitud membran cholesterol mol occur erythrocyt p rise cms c p suffici ensur complet load passag erythrocyt lung capillari bed condit rest well maxim exercis

D002463;D010101

T043;T201

acut chang myocardi express heat shock protein apoptot respons follow blood delnido custodiol cardioplegia infant undergo open heart surgeri stress caus cardiopleg ischem arrest shown alter express level heat shock protein hsp littl known effect particular pediatr heart studi aim investig whether myocardi cellular stress apoptot respons chang due differ cardioplegia cp solut cardiopulmonari bypass cpb infant determin influenc surgicalclin outcom therefor twenti seven infant surgic closur ventricular septal defect random assign cp solut normotherm blood bcp delnido dncp custodiol ccp hsp level apoptosi determin immunoblot cardiac tissu right atrium cp correl cardiac paramet evalu signific chang observ hsp level hsp hsp hsp level decreas signific bcp group increas mark ccp group decreas hsp increas hsp express detect dncp group import apoptosi observ dncp ccp group wherea mark increas cleav caspas determin bcp serum cardiac troponin ctn myocardi injuri marker mark lower bcp dncp group ccp addit hsp hsp hsp level posit correl aortic cross clamp time total perfus time ctn releas find show dncp provid effect myocardi preserv pediatr open heart surgeri indic increas hsp express may associ cardioprotect effect increas hsp hsp level may indic myocardi damag cpb

D006348;D006324

T061

control genom smartphon first clinic experi promis system develop precis medicin strategi requir high dimension phenotyp genom data high privaci sensit data type convent data manag system lack capabl suffici handl expect larg quantiti sensit data secur manner promis genet data manag concept implement high secur platform data exchang preserv patient interest privaci autonomi

D016494;D000068997

T057;T073;T097;T170;T078;T080

increas preval gestat diabet mellitus carri variant allel mthfr gene ct polymorph systemat review meta analysi previous epidemiolog data link ct ac mthfr polymorph gestat diabet risk mix controversi therefor conduct meta analysi deriv precis estim relationship mthfr polymorph pregnanc disord

D016640

T047

bleed pattern medic manag earli pregnanc loss mifepriston misoprostol prognost valu prospect observ cohort studi improv counsel women report bleed characterist home medic manag earli pregnanc loss epl mifepriston misoprostol evalu occur bleed pattern prognost tool

D000020;D000028;D000022;D016595

T047;T121;T109;T046;T093;T061;T081;T033

juvenil granulosa cell tumor pregnanc case seri literatur review pregnanc complic juvenil granulosa cell tumor jgct rare thus experi clinic diagnosi manag limit

D006106;D010051

T191

impact wear face mask public prevent infecti diseas psychosoci develop children adolesc systemat review wear face mask public recommend certain circumst order prevent infecti diseas transmit droplet

D000086382;D003141

T047;T067

endocardi left ventricular pace cardiac resynchron therapi crt effect treatment dyssynchron heart failur howev patient fail improv implant endocardi left ventricular lv pace altern therapi patient respond convent crt placement lead via coronari sinus possibl enabl pace wide varieti site without restrict due coronari sinus anatomi evid superior electr resynchron hemodynam respons compar convent epicardi crt articl discuss potenti advantag disadvantag endocardi lv pace compar convent crt review evid deliveri endocardi lv pace use lead base leadless system explor possibl futur direct novel technolog

D058406;D006333

T047;T061;T046

prognosi valu serum solubl st level acut ischem stroke stemi patient era mechan reperfus therapi solubl form suppress tumorigen sst known prognost valu st elev myocardi infarct stemi could impact mortal acut ischem stroke ai howev consid sst therapeut target kinet releas associ advers clinic event stemi ai patient determin

D000083242;D000072657

T047

influenc clinic trial decis make process patient amyotroph later sclerosi amyotroph later sclerosi al incur neurolog diseas patient diagnos al surviv time year without life sustain therapi decis make process accept declin percutan endoscop gastrostomi peg tracheostomi invas ventil tiv therapi complex multifacet studi examin whether particip particip clinic trial al influenc decis make process al patient

D000690;D002986;D003657

T062;T047;T041

cannabi user mental travel time evid fmri studi episod futur think episod futur think eft cognit function allow individu imagin novel experi may happen futur prior studi show eft impair differ group substanc user howev evid regard neurobiolog mechan eft cannabi user

D002188;D061212

T121;T002;T109;T167;T041

extracorpor cardiopulmonari resuscit treatment hospit cardiac arrest hospit cardiac arrest ohca affect ca peopl year germani associ limit prognosi high mortal extracorpor cardiopulmonari resuscit ecpr use arterioven extracorpor membran oxygen av ecmo system addit option treatment increas widespread sinc anchor guidelin algorithm

D016887;D015199;D058687

T046;T061

risk factor rate recurr moh surgeri basal cell squamous cell carcinoma nationwid prospect cohort regesmoh spanish registri moh surgeri random studi assess efficaci moh micrograph surgeri basal cell squamous cell carcinoma limit methodolog ethic issu lack long follow period studi present real life result nationwid year cohort basal cell carcinoma squamous cell carcinoma treat moh micrograph surgeri prospect cohort conduct spanish centr juli februari multivari analysi includ characterist patient tumour surgeri follow perform total patient follow patient year basal cell carcinoma patient patient year follow squamous cell carcinoma recruit risk factor recurr includ age non primari tumour stage unfinish surgeri tumour immunosuppress squamous cell carcinoma incid rate recurr per person year basal cell carcinoma confid interv squamous cell carcinoma confid interv constant time year conclus follow strategi equal intens least first year special attent paid squamous cell carcinoma especi immunosuppress patient elder patient non primari tumour procedur requir stage unfinish surgeri

D002280;D002294;D012878

T191

pleomorph onychomatricoma mimick malign pleomorph onychomatricoma rare condit mimick malign neoplasm given rariti diagnost prognost criteria condit well establish aim studi character seri case pleomorph onychomatricoma case submit clinic diagnosi subungu squamous cell carcinoma case nail clip show typic featur onychomatricoma free edg thicken pit nail plate addit featur project line pattern pleomorph onychomatricoma diagnos base moder sever cytolog atypia yet degen appear multinucl smudg chromatin mitot activ necrosi ki prolif index inferior overal find includ epithelioid multinucl cell deepli eosinophil cytoplasm mimick epithelioid malign cell overexpress ki p atyp cell diffus express p studi describ addit criteria pleomorph onychomatricoma permit wider recognit order avoid inappropri treatment

D002294;D009260;D012878

T191;T047

asymptomat sar cov infect associ miscarriag earli pregnanc retrospect analysi preval sar cov infect pregnanc relat unknown studi report potenti impact undiagnos sar cov infect pregnanc loss first half pregnanc compar preval infect retrospect group pregnant women miscarriag n prospect control group pregnanc loss first trimest n women miscarriag result igm sar cov negat igg seroposit pregnant women result igm negat igg seroposit sar cov seropreval signific differ two group women therefor exclud signific role sar cov infect pregnanc loss therefor data show sar cov infect within first trimest seem predispos earli pregnanc loss impact asymptomat mild symptomat sar cov infect pregnanc appear limit

D000022;D000086382;D011251

T033;T047;T067;T046

effect differ fatti acid neisseria gonorrhoea viabil neisseria gonorrhoea gc agent one common bacteri sexual transmit infect worldwid possibl develop untreat infect point need antibiot spare method reduc number gonococc infect context fatti acid interest candid next generat antibacteri agent aim studi investig bactericid effect select fatti acid gc viabil well observ biolog effect mean transmiss electron microscopi cytotox compound human cervic cell hela chosen model genit mucosa assess well lauric myrist palmit acid display high kill activ gc concentr rang wherea antimicrobi effect oleic butyr acid present concentr mm mm modif induc fatti acid gc cell includ disorgan cytoplasm structur distort pilifimbria separ inner outer membran layer concentr activ gc fatti acid toxic cervic cell data help promot innov antibiot free compound treatment gc infect

D006069;D009344

T007;T047

molecular diagnosi sar cov perform throughput direct rt pcr covid pandem requir laboratori rapid unexpect reorgan cope increas request test rapid respons time least provid shorten molecular reagent order valid accur faster cheaper method suitabl larg scale diagnosi sar cov evalu simplifi workflow direct rt pcr nasopharyng swab seegen autom platform direct rt pcr ensur overal concord versus standard rna rt pcr sampl ct valu save extract reagent provid approxim three fold increas product hour

D000086382;D000086402

T047;T005;T067

effect neutral deterg fiber digest estim method calcul energi concentr canola meal canadian process plant aim determin whether method use estim truli digest neutral deterg fiber tdndf affect calcul concentr total digest nutrient tdnx net energi lactat nelx canola meal cm sampl collect cm process plant canada yr n analyz dri matter dm crude protein cp ether extract ee ash neutral deterg fiber ndf acid deterg fiber adf lignin adl neutral deterg insolubl cp ndicp rumin situ incub cm sampl perform h determin ndf fraction b c effect rumin ndf digest erndfd indigest ndf indf cm three tdndf estim method evalu nation research council nrc ndf ndicp adl adl ndf ndicp indf ndf ndicp ndf remain h situ erndfd estim situ ndf digest kinet result tdndf valu use calcul tdnx nelx accord nrc equat data analyz mix procedur sas determin effect process plant chemic composit ndf degrad kinet nelx cm effect tdndf estim method calcul tdnx nelx cm also evalu model analysi process plant includ fix effect plant random effect year plant replic analysi tdndf method includ fix effect tdndf estim method random effect process plant year plant replic effect process plant dm p cp p ee p ndf p cm process plant also effect ndf fraction p b p affect fraction c erndfd tdndf estim method effect tdndf p tdnx p nelx p cm yield averag nelx valu mcalkg nrc indf erndfd respect result indic calcul energi concentr cm accord nrc equat vari depend method use estim tdndf research need determin accur estim method

D000821;D003902

T120;T121;T168;T073

video polysomnographi procedur diagnosi rapid eye movement sleep behavior disord rbd identif prodrom stage guidelin intern rbd studi group video polysomnographi v psg essenti diagnos rapid eye movement rem sleep behavior disord rbd although current american academi sleep medicin standard diagnos rbd sever aspect need address achiev harmon across sleep center prodrom rbd stage symptom sign evolv rbd present yet meet establish diagnost criteria rbd howev boundari prodrom definit rbd still unclear common effort neurophysiolog work group intern rbd studi group manuscript address need comprehens unambigu v psg recommend diagnos rbd identifi prodrom rbd includ standard v psg technic set specif consider rem sleep score harmon method score rem sleep without atonia consist method analyz video audio record v psgs classifi movement vocal clear v psg guidelin diagnos rbd identifi prodrom rbd section follow common templat current recommend method present limit outlin new recommend describ final futur direct present v psg recommend intend practic clinician research classif quantif motor event rbd episod vocal howev intend research purpos v psg guidelin allow collect homogen data provid object v psg measur make futur harmon multicentr studi clinic trial possibl

D020187

T048

high parallel genom select respons replic drosophila melanogast popul reduc genet variat mani adapt trait polygen frequent loci contribut phenotyp segreg need express phenotyp optimum experiment evolut replic popul adapt new control environ provid power approach studi polygen adapt genet redund often result nonparallel select respons among replic propos modifi evolv resequ e r design maxim similar among replic rather start mani founder use two inbr drosophila melanogast strain expos extrem hot temperatur environ c generat detect mani genom region strong high parallel select respons evolv replic x chromosom pronounc select respons autosom may attribut domin effect furthermor find median select coeffici chromosom higher two genotyp experi classic e r studi two random genom harbor suffici variat adapt respons propos approach particular well suit analysi polygen adapt

D004331;D023281

T091;T204

character metalloproteom pseudoalteromona bb biogeochem underpin zinc manganes cobalt nickel cycl ubiquit marin heterotroph pseudoalteromona bb ubiquit marin heterotroph often associ labil organ carbon sourc ocean eg phytoplankton bloom sink particl heterotroph hydrolyz export photosynthet materi compon biolog carbon pump use divers metalloenzym contain zinc zn manganes mn cobalt co nickel ni studi metal requir cytosol util metal marin heterotroph scarc despit relev global carbon cycl character zn mn co ni metallom bb found zn metallom complex cytosol zn associ numer protein transcript metallom obtain singular valu decomposit metalloproteom data translat proteolysi alkalin phosphatas activ numer proteolyt enzym also appear putat associ mn lesser extent co putat identif ni associ protein phosphoglucomutas protein cupin superfamili provid new insight ni util marin heterotroph bb reli numer transit metal proteolyt phosphatas activ infer adapt potenti metal limit field observ increas alkalin phosphatas activ upon addit zn field incub suggest metal limit oper sink particul materi collect sediment trap taken togeth studi improv understand zn mn co ni metallom marin heterotroph bacteria provid novel mechanist framework understand influenc nutrient limit biogeochem cycl

D008386;D020543

T116;T090;T123

pheromon deploy strategi mate disrupt vineyard mealybug mealybug planococcus ficus signoret primari vineyard pest california grape grow region throughout world mate disrupt program commerci avail manag pl ficus widespread adopt limit part high cost compar insecticid program improv mate disrupt econom effect differ deploy technolog passiv aerosol microencapsul formul individu examin adult male pl ficus captur pheromon trap categor rate vine infest crop damag suggest deploy strategi lower mealybug densiti damag use passiv dispens deploy rate per ha lower pl ficus crop damag similar per ha rate common use commerci oper reduc rate would lower product deploy cost meso dispens contain ai deploy per ha treatment impact microencapsul formul aerosol canist lower male flight captur andor crop damag male mealybug flight activ greatest hr coincid temperatur c restrict time temperatur suggest program dispens might allow pheromon deploy coincid flight pattern larg field trial use passiv dispens found greater treatment separ yr pheromon deploy discrep result among vineyard may relat pl ficus densiti combin result trial suggest differ deploy technolog use impact pl ficus densiti damag even reduc rate especi continu use multipl season

D009036;D012724

T204;T123

local thermal environ warm influenc supercool drive widespread shift metabolom diapaus pieri rapa butterfli global climat chang potenti negat impact biolog system organ expos novel temperatur regim increas annual mean temperatur accompani disproportion rate chang temperatur across season winter season warm rapid yet know relat littl warm alter physiolog overwint organ simul futur warm condit compar diapaus pieri rapa butterfli pupa collect dispar thermal environ expos p rapa pupa acut chronic increas temperatur first compar intern freez temperatur supercool point diapaus pupa develop common garden condit whose parent collect northern vermont usa north carolina usa match warmer winter climat north carolina north carolina pupa signific higher supercool point vermont pupa next measur effect acut chronic warm exposur vermont pupa found warm induc higher supercool point character effect chronic warm profil metabolom vermont pupa via untarget lc ms metabolom warm caus signific chang abund hundr metabolit across metabolom notabl warm induc shift key biochem pathway pyruv metabol fructos mannos metabol alanin metabol suggest shift energi metabol cryoprotect result suggest warm affect various aspect overwint physiolog p rapa may detriment depend frequenc variat winter warm event research need ascertain extent effect warm felt among broader set popul p rapa among speci order better predict insect may respond chang winter thermal environ

D002080

T204

lower statist support larger data set insight ochrophyta radiat common assum increas number charact potenti resolv evolutionari radiat studi photosynthet stramenopil ochrophyta use align heterogen origin mitochondrion plastid nucleus surpris statist support relationship six major ochrophyta lineag increas compar mitochondrion site plastid site tree decreas nuclear site tree statist support simpli relat data set size also quantiti phylogenet signal avail posit abil extract show abil current phylogenet method limit conflict result obtain vari taxon sampl even though use better fit model improv signal extract reduc observ conflict plastid data set provid higher statist support ochrophyt radiat larger nucleus data set propos higher support observ plastid tree due acceler evolutionari rate one short deep intern branch impli phylogenet signal per posit avail resolv ochrophyta radiat plastid nuclear data set work therefor suggest order resolv radiat beyond obvious use data set posit need continu develop model sequenc evolut better extract phylogenet signal design method search genescharact contain signal specif short intern branch

D058009

T204

thermal histori alfalfa leafcut bee affect nest diapaus incid variabl spring temperatur may expos develop insect subleth condit result long term consequ alfalfa leafcut bee megachil rotundata overwint prepupa insid brood cell resum develop spring immobil stage develop bee must toler unfavor temperatur studi test exposur low temperatur stress develop affect subsequ reproduct characterist f generat develop male femal rotundata expos either constant c fluctuat h day c low temperatur stress week pupal stage mimic spring cold snap treat adult mark releas field cage reproduct output compar untreat control bee exposur low temperatur pupal stage mix effect reproduct offspr characterist femal treat fluctuat low temperatur like nest compar control bee expos constant low temperatur stress subleth effect may contribut low nest rate bee expos constant low temperatur femal group abl nest fewer larger offspr high viabil suggest trade interest offspr bee expos fluctuat low temperatur like enter diapaus indic thermal histori parent even develop import factor diapaus determin

D000074767;D000455

T040;T002

adar edit virus evolutionari forc reckon adenosin deaminas act rna adar rna edit enzym play dynam nuanc role regul transcriptom proteom divers edit high select affect specif site within transcript nonselect result hyperedit adar edit import regul neural function autoimmun key role innat immun respons viral infect edit rang pro antivir effect contribut viral evolut examin role adar edit across broad rang viral group propos effect adar edit viral replic whether pro antivir better view axi rather binari specif posit given virus axi high depend virus host specif factor chang cours infect howev research need devot understand dynam factor affect virus adar interact viral evolut anoth area warrant signific attent effect virus adar interact host adar interact particular light crucial role adar regul neural function answer question essenti develop understand relationship adar edit viral infect turn understand effect virus sar cov well mani other therebi influenc approach treat dead diseas

D017393

T045

smoke enhanc suicid risk signific role covid pandem covid outbreak sever affect whole world consider evid suggest tobacco smoke associ increas sever covid death covid patient tobacco smoke cessat necessari decreas covid relat hospit death commentari suggest tobacco smoke cessat also need reduc suicid behavior covid pandem signific evid suggest covid pandem lead increas tobacco consumpt smoker use tobacco cope pandem relat stress anxieti depress loneli multipl studi demonstr tobacco smoke associ suicid ideat suicid attempt suicid death contribut factor pathophysiolog suicid smoke may increas probabl develop post covid syndrom increas sever covid suicid risk may increas individu post covid syndrom smoke prevent cessat target suicid prevent intervent covid pandem covid pandem enhanc need act integr tobacco smoke cessat health care standard patient care

D000086382;D058873

T047;T067

multidimension genom wide screen yeast provid mechanist insight europium toxic europium lanthanid metal high valu optoelectron even though europium use mani commerci product toxicolog profil partial character studi focus identifi lethal dose differ system bioaccumul vivo paper describ genom wide toxicogenom studi europium saccharomyc cerevisia share mani biolog function human use multidimension approach function network analys identifi group gene protein associ yeast respons amelior metal toxic includ metal discharg path vesicl mediat transport path regul biolog relev cation process reduc metal induc stress furthermor analys indic europium promot yeast toxic disrupt function chaperon cochaperon metal bind site sever gene protein highlight studi human orthologu suggest may particip europium induc toxic human identifi endogen target europium well alreadi exist path decreas toxic determin specif gene protein may help develop futur therapeut strategi

D016681

T028

advers childhood experi subsequ chronic diseas among middl age older adult china associ demograph socioeconom characterist associ advers childhood experi ace chronic diseas among middl age older chines individu well document addit whether demograph socioeconom characterist modifi associ underexplor

D012923

T080;T098

mutat select model protein evolut persist posit select use first principl popul genet model evolut protein persist posit select pps pps may occur organ subject persist environment chang adapt radiat host pathogen interact mutat select model indic protein evolut pps irrevers markov process thus protein pps show strong asymmetr distribut select coeffici among amino acid substitut model show criteria ratio nonsynonym synonym codon substitut rate detect posit select conserv inde arbitrari real protein mani mutat high deleteri remov select even posit select site use penal likelihood implement pps model success detect pps plant rubisco influenza ha protein direct estim select coeffici protein site infer procedur bypass need use surrog measur select improv abil detect molecular adapt protein

D008957;D012641

T070;T170;T045

plasma base strategi inhibit candida albican growth camca gene express vitro reduc fungal pathogen murin model vulvovagin candidiasi vulvovagin candidiasi vvc common mucos infect main caus candida albican use common antifung drug treatment vvc limit due emerg resist fungal strain sever side effect cold atmospher plasma cap novel therapeut approach proven display strong antifung activ c albican present studi effect cap treatment virul pathogen c albican murin model investig candida albican treat cap differ time exposur fungal cell morpholog express profil camca gene cap treat fungus evalu use electron microscopi quantit rt pcr moreov mice model vvc develop use cap treat non treat c albican character term vagin fungal burden rate hypha format vagin tissu fluid inflamm degre mice vagin tissu signific reduct camca express remark mitochondri degrad observ cap treat c albican cell lowest fungal burden reduc hypha format poor adher yeast cell vagin epithelium low degre inflamm observ mice infect cap treat c albican suppress camca gene mitochondri degrad cap treat c albican yeast cell may diminish yeast hypha transit reduc fungal pathogen murin model vvc cap treatment consid novel effici therapeut strategi c albican relat candida infect practic

D002181;D004364;D012376

T047;T121

electron cigarett use among adolesc european studi site find global youth tobacco survey new find public health implic electron cigarett e cigarett use emerg surveil remain utmost import studi examin latest state e cigarett use youth european studi site ie countri feder bosnia herzegovina use global youth tobacco survey gyt

D066300;D062789;D000072137

T055;T131;T073;T109

dietari caroten influenc ontogenet colour chang southern corrobore frog ontogenet colour chang occur divers vertebr taxa may close link dietari chang throughout develop various speci red orang yellow colour enhanc consumpt carotenoid howev pauciti long term dietari manipul studi mean littl known role individu carotenoid compound ontogenet colour chang know even less influenc individu compound differ dose dose effect present studi aim use larg dietari manipul experi investig effect dietari caroten supplement colour southern corrobore frog pseudophryn corrobore earli post metamorph develop frog rear four dietari treatment four caroten concentr mg g frog colour measur everi week week caroten found influenc colour dose howev colour found becom conspicu time includ control treatment moreov frog express colour maxim similar point develop result impli studi speci caroten may contribut littl noth colour frog manufactur colour colour develop continu process may select synchronis develop colour express discuss potenti adapt benefit ontogenet colour chang p corrobore broad draw attent potenti adapt development synchroni express colour aposemat speci

D001001;D019207

T011;T127;T121;T109

electrocardiogram monitor prone posit coronavirus diseas acut respiratori distress syndrom prone posit increas use treat coronavirus diseas covid induc acut respiratori distress syndrom ard high risk patient cardiovascular event may spend h day prone posit adequ monitor electrocardiogram ecg mandatori howev effect prone posit ecg unknown valid ecg record electrod place dorsal aim compar ecg data obtain prone posit five electrod posit convent dorsal assess effect chang posit supin prone ecg patient covid ard

D000086382;D012128

T047;T067

compar echocardiograph characterist genotyp posit phenotyp posit hypertroph cardiomyopathi hypertens left ventricular hypertrophi littl inform hypertroph cardiomyopathi hcm patholog genet mutat concurr hypertens htn hypertens left ventricular hypertrophi lvh exclud under genet aetiolog

D002312;D006973

T047

visual echocardiograph score system left ventricular fill pressur outcom heart failur preserv eject fraction elev left ventricular fill pressur lvfp power indic worsen clinic outcom heart failur preserv eject fraction hfpef howev detect elev lvfp often challeng studi aim determin associ newli propos echocardiograph lvfp paramet visual assess time differ mitral valv tricuspid valv open vmt score clinic outcom hfpef

D001281;D006333

T047;T046

phenotyp hypertroph cardiomyopathi use cardiac diffus magnet reson imag relationship microvascular dysfunct microstructur chang microvascular dysfunct hypertroph cardiomyopathi hcm predict clinic declin howev under mechan remain unclear cardiac diffus tensor imag cdti allow vivo character myocardi microstructur quantifi mean diffus md fraction anisotropi fa diffus secondari eigenvector angl ea cardiac magnet reson cmr studi examin associ perfus cdti paramet understand sequenc pathophysiolog interrel vascular function under microstructur

D002312;D056324

T047;T060

piezo pannexin complex coupl forc detect atp secret cholangiocyt cholangiocyt activ contribut final composit secret bile cell expos abnorm mechan stimuli obstruct cholestasi deep impact function howev effect mechan insult cholangiocyt function understood combin gene silenc pharmacolog assay live calcium imag probe molecular candid essenti coupl mechan forc atp secret mous cholangiocyt show piezo pannexin necessari elicit downstream effect mechan stress mediat rise intracellular ca piezo act mechanosensor respons translat cell swell activ panx trigger atp releas subsequ signal amplif pxr co immunoprecipit pull assay indic physic interact piezo panx lead stabl plasma membran complex piezo panx pxr atp releas pathway could reconstitut hek piezo ko cell thus data suggest piezo panx form function signal complex control forc induc atp secret cholangiocyt find may foster develop novel therapeut strategi biliari diseas

D000255;D017630;D004847;D007473;D009419

T116;T121;T123;T025;T114

chemic biolog studi natur synthet product high select control pest insect speci tanacetum cinerariifolium known produc pyrethrin mechan pyrethrin biosynthesi larg unclear author show nonmevalon oxylipin pathway underli biosynthesi acid alcohol moieti respect gdsl lipas join product pathway blend green leaf volatil e farnesen mediat induct wound respons neighbor intact conspeci enhanc pyrethrin biosynthesi plant fight herbivor underground well aboveground soy pulp fungi produc compound select modul ion channel insect nervous system author propos indirect defens plant occur microorgan produc defens substanc rhizospher broad spectrum pesticid includ neonicotinoid may affect nontarget organ author discov cofactor enabl function express insect nicotin acetylcholin receptor nachr led understand mechan insect nachr neonicotinoid interact thus pave new avenu control crop pest diseas vector

D011722

T131;T109

effect radiotherapi expand perman implant immedi breast reconstruct long term surgic patient report outcom larg multicentr cohort current evid effect radiotherapi rt implant base immedi breast reconstruct ibr limit short follow lack patient report outcom pros central integr long term comprehens outcom data preoper decis make process aim present studi determin long term surgic outcom pros relat rt implant base ibr

D018427;D016462;D015625

T074;T061

pathway microcirculatori endotheli dysfunct obstruct sleep apnea comprehens ex vivo evalu human tissu mechan marker cardiovascular diseas cvd obstruct sleep apnea osa remain unknown microcircul site earli chang osa patient free cvd risk

D020181

T047

left ventricular dysfunct among patient embol stroke undetermin sourc effect rivaroxaban vs aspirin subgroup analysi navig esus random clinic trial uncertain whether anticoagul superior aspirin reduc recurr stroke patient recent embol stroke undetermin sourc esus left ventricular lv dysfunct

D018487

T046

uspstf approach address sex gender make recommend clinic prevent servic clinic prevent servic recommend us prevent servic task forc uspstf base transpar systemat rigor method consid certainti evid magnitud net benefit guidelin aim address need divers popul biolog sex gender ident sourc divers often consid studi clinic prevent servic inform recommend result challeng evalu evid communic recommend person specif gender identif categori manwomangend nonbinarygend nonconformingtransgend advanc method uspstf review past recommend includ use sex gender term review approach guidelin make bodi pilot test strategi address sex gender divers base find uspstf intend use inclus approach identifi issu relat sex gender start guidelin develop process assess applic variabl qualiti evid function sex gender ensur clariti use languag regard sex gender identifi evid gap relat sex gender evid review identifi limit appli find divers group under studi use unclear terminolog regard sex gender uspstf use gender neutral languag appropri communic recommend inclus peopl gender clear state recommend appli individu specif anatomi associ biolog sex malefemal specif categori gender ident uspstf recogn limit evid inform prevent care popul base gender ident

D005783;D011314;D012723

T058;T032;T041

cell cycl independ integr stress signal xbp promot non gg quiescenc entri cellular quiescenc nonprolif state requir cell surviv stress develop quiescent cell prolifer stop revers state low cdk kinas activ mani organ howev quiescent state high cdk activ also establish still uncharacter stress development mechan use microfluid approach coupl phenotyp classif machin learn identifi stress pathway associ starvat trigger high cdk quiescent state saccharomyc cerevisia found low high cdk quiescent state share core stress associ process autophagi protein aggreg mitochondri regul differ nuclear accumul stress transcript factor xbp gln sfp decis low high cdk quiescenc control cell cycl independ accumul xbp act time delay integr durat stress stimuli result show cell cycl independ stress activ factor promot cellular quiescenc outsid gg

D002453;D015398;D013312

T044;T121;T043;T039;T046;T032;T040

increas depth reduc extent sharpen edg visual field defect measur compass fundus perimet compar humphrey field analyz purpos studi compar visual field result compass fundus perimet cmp humphrey field analyz hfa eye compar structur function concord circumpapillari retin nerv fiber layer cp rnfl profil two perimetri result evalu whether differ two result reflect postul advantag real time eye movement compens perimetri

D005901;D058609

T047;T060

long term health social outcom children adolesc place home care children place home care may poorer outcom adulthood averag compar peer direct magnitud associ need clarif

D013995

T079;T067

structur activ relationship aromat moieti substitut dihydro h pyran one find novel compound show higher plant growth inhibitori activ cours research structur activ relationship dihydro h pyran one r hydroxi fluorophenyl hexyl dihydro h pyran one found show fold potent plant growth inhibitori activ italian ryegrass shoot ic root ic compound bear unsubstitut phenyl group small electron withdraw atom posit benzen ring caus higher activ

D011714

T109

causal futur influenc shape featur caus extern transform visual attent previous studi valid particip distinguish differ origin object shape featur teas apart featur caus transform causal histori origin shape consid bite transform exampl two experi design investig effect causal histori alloc visual attent particip present regular familiar complet bitten shape experi unfamiliar irregular complet bitten shape experi rang stimulus onset asynchroni soa task identifi differ probe ie punctuat mark equal appear four posit around shape result show complet regular shape impact particip reaction time identifi probe appear four differ posit experi wherea complet irregular shape would facilit particip respons probe appear posit around head irregular shape experi regardless soa present bitten shape earlier phase visual process particip respons pattern resembl found complet shape present howev longer soa particip faster identifi probe appear posit around nontransform region bitten shape result reveal inform shape featur caus causal histori could incorpor albeit relat later alloc visual attent role causal histori specul one object futur develop discuss

D010364;D014796

T041

general critic space crowd optotyp bouma st centuri rare find crowd manuscript fail mention bouma law rule thumb state flanker within distanc one half target eccentr induc crowd investig general rule even optotyp factor modul critic space evid rule bouma data explor question reanalyz varieti studi literatur run sever new control experi util model unifi flank identif measur psychophys paradigm specif minim assumpt equival psychometr slope across condit exampl crowd acuiti predict arbitrari target size flanker space reveal perform landscap delin critic space last present compact quantit summari effect differ type stimulus manipul optotyp crowd

D003441;D010364

T041;T054

cnm bridg mitochondria nuclear er membran contact site defin molecular level use high throughput microscopi base screen eisenberg bord zung et al j cell biolhttp doiorgjcb identifi cnm novel tether protein mediat contact mitochondria nuclear er respons phospholipid level

D008928;D051336

T043;T026

assess nonfat myocardi infarct surrog caus cardiovascular mortal treatment prevent coronari arteri diseas meta analysi random clinic trial although nonfat myocardi infarct mi associ increas risk mortal evid valid nonfat mi surrog end point caus cardiovascular cv mortal lack

D016032

T062;T170

px receptor nitric oxid interact mediat apoptosi mous immort mesangi cell expos high glucos diabet mellitus dm chronic diseas character hyperglycemia lead diabet nephropathi dn show px purinerg receptor high express dm howev oxid stress control renal recov activ receptor remain signific reduc aim studi assess influenc px apoptosi mous immort mesangi cell mimc cultur high glucos hg medium

D003928;D006943

T033;T047

simpl colorimetr method viabl bacteria detect base cell count kit studi cell count kit cck introduc detect concentr live bacteria first time depend redox reaction cck solut dehydrogenas cck solut reduc form water solubl orang yellow formazan dehydrogenas present bacteri cell concentr live bacteria proport absorb valu formazan nm base principl staphylococcus aureus escherichia coli chosen model bacteria optim detect condit investig good linear relationship obtain concentr rang cfu ml linear equat log x x cfu ml r aureus cfu ml linear equat log x x cfu ml r e coli cck base viabl bacteria detect method complet within h wide bacteri detect concentr rang satisfactori result obtain appli actual sampl analysi good consist propos cck base method tradit plate count method import method realiz one time detect larg number sampl high sensit suggest great potenti high throughput bacteri detect

D003124

T059

possibl regul liver glycogen structur gut liver axi resist starch review liver glycogen particl diabet patient fragil relat healthi individu restor fragil glycogen particl normal state show potenti contribut remiss diabet resist starch rs benefici diabet manag interact gut microbiota howev effect glycogen fragil fulli understood review aim summar recent understand interact rs human gut microbiota possibl connect liver glycogen biosynthesi elucid role develop glycogen fragil rs might regul glycogen fragil diabet modul postprandi glycem respons glycogen biosynthesi pathway rs appli repair fragil glycogen work done better understand vivo rs structur identifi factor bind glycogen particl togeth review contain import inform connect glycogen fragil rs gut microbiota interact could help better understand health benefit rs consumpt

D000069196;D008112;D000084922

T007;T121;T109;T123;T001

macrophag hitchhik supramolecular aggreg cus nanoparticl enhanc tumor deposit phototherm therapi design small cus nanoparticl nps intracellular self assembl larg supramolecular aggreg via host guest interact sequenti intern cyclodextrin cap cus nps ferrocen cap cus nps insid macrophag thus efflux cus nps signific inhibit macrophag hitchhik deliveri biodistribut studi mice confirm dramat enhanc deposit cus nps tumor tissu mice inject macrophag carri intracellular cus aggreg comparison mice treat macrophag carri cus nps respons inflammatori tumor microenviron oxid ferrocen would dissoci cyclodextrin ferrocen host guest pair drive disassembl cus aggreg releas small cus nps deep tissu penetr enhanc phototherm therapi precis control intracellular self assembl disassembl nanomedicin insid macrophag provid novel cell hitchhik deliveri strategi minim prematur leakag nanomedicin also great improv deliveri effici tumor penetr safe effect tumor therapi

D053758;D009369

T191;T073

strategi use natur fix red purpl blue colour plant physic chemistri approach identifi respect flavylium cation anthocyanin much molecul flavylium cation general appear acid ph valu one molecul complex sequenc ph depend molecular speci revers interconnect differ chemic reaction speci includ red flavylium cation purpl quinoid base blue bluish anion quinoid base common ph vacuol simpler anthocyanin red flavylium cation present acid ph valu moder acid phs signific colour purpl quinoid base moreov blue bluish anion quinoid base appear around neutral ph valu stabl intermolecular copigment intramolecular acyl anthocyanin interact increas colour hue yield bathochrom shift absorpt band permit extend ph domain flavylium cation increas mole fraction quinoid base metal complex anoth strategi particular al cation play essenti role blue colour hydrangea sophist structur howev metaloanthocyanin one give blue colour commelina communi constitut six anthocyanin six flavanon two metal work discuss physic chemic tool indispens account chemic behaviour complex system experiment procedur equat need calcul equilibrium constant anthocyanin consequ ph depend mole fraction distribut absenc presenc copig describ detail revers ph jump monitor stop flow shown indispens tool calcul paramet

D003116

T080

kinet parallel consecut bimolecular reaction solut invers problem involv lambert w function parallel consecut bimolecular reaction mechan solut invers kinet problem approach direct use characterist equat specifi term lambert w function similar logarithm reciproc plot treatment simpl first second order reaction kinet respect

D000465

T170

format asymmetr belt like aggreg bio base surfact deriv dehydroabiet acid morpholog physicochem properti order molecular aggreg close relat surfact molecul herein rosin base amin oxid surfact contain larg hydrophob group abbrevi r ao synthes dehydroabiet acid import deriv rosin cryogen transmiss electron microscopi cryo tem imag small angl x ray scatter sax show concentr mm r ao molecul form flexibl nanobelt thick nm width nanobelt nm length format stabl nanobelt aros strong van der waal forc bulki hydrophob portion r ao solut facilit stabil asymmetr aggreg rheolog test show form nanobelt thermodynam stabl entangl nanobelt led signific viscoelast solut zero shear viscos r ao solut reach pa concentr mm much greater wormlik micellar solut work provid inspir prepar aggreg novel properti use natur product

D045784;D013501

T120;T122;T109

generat cellular spheroid use dna modifi cell receptor programm dna interact cultur known provid faith tissu model cultur thus valuabl tool vitro evalu biolog model howev mani cell line unabl form desir spheroid tradit method natur occur cell cell adhes weak present method produc cell spheroid use dna mediat assembl first demonstr affin mediat photoconjug approach amcp coval modifi cell receptor affibodi streptavidin fusion protein affibodi chemic crosslink cell express egfr streptavidin use attach dna strand dna conjug cell mix complementari dna linker strand impart cell cell interact incub well coat non adhes polym cell form dens spheric aggreg larger micron diamet studi carri use human breast cancer cell mba mb aneuploid human keratinocyt hacat human colon cancer cell caco without either dna cell solut linker cell spheroid observ h incub cultur dna assembl spheroid found mechan stabl enough handl easili analysi confoc imag find suggest propos dna assembl method consid attract strategi assembl cell stabl spheroid

D002450;D018874

T043;T025

monitor gut acidif adult drosophila intestin fruit fli midgut consist multipl region compos cell carri uniqu physiolog function requir proper function gut one region copper cell region ccr local middl midgut consist part group cell known copper cell copper cell involv gastric acid secret evolutionarili conserv process whose precis role poor understood paper describ improv current protocol use assay acidif adult drosophila melanogast gut demonstr use speci fli particular paper demonstr gut acidif depend fli nutrit status present protocol base new find overal protocol demonstr potenti use studi drosophila copper cell uncov general principl under mechan gut acidif

D004330;D029721

T204;T116;T123

use optogenet revers neuroplast inhibit cocain seek rat protocol demonstr step need use optogenet tool revers cocain induc plastic thalamo amygdala circuit reduc subsequ cocain seek behavior rat research found rat self administ intraven cocain pair audiovisu cue synaps form input medial genicul nucleus thalamus mgn onto princip neuron later amygdala la becom stronger cue cocain associ learn hypothes revers cocain induc plastic synaps would reduc cue motiv cocain seek behavior order accomplish type neuromodul vivo want induc synapt long term depress ltd decreas strength mgn la synaps end use optogenet allow neuromodul brain circuit use light excitatori opsin ochief express presynapt mgn termin la infus aav contain ochief mgn optic fiber implant la nm laser light puls frequenc hz minut induc ltd revers cocain induc plastic manipul produc long last reduct abil cue associ cocain induc drug seek action

D003042;D019970

T131;T048;T121;T109

stimul analyz adult neurogenesi drosophila central brain molecular cellular mechan under neurogenesi respons diseas injuri well understood howev understand mechan crucial develop neural regen therapi drosophila melanogast lead model studi neural develop histor exploit investig adult brain regener primarili adult brain exhibit low mitot activ nonetheless penetr traumat brain injuri ptbi adult drosophila central brain trigger generat new neuron new glia power genet tool avail drosophila combin simpl rigor injuri protocol describ make adult drosophila brain robust model neural regener research provid detail instruct penetr injuri adult central brain dissect immunohistochemistri imag post injuri protocol yield high reproduc result facilit addit studi dissect mechan under neural regener

D004330;D004331

T204

isol murin retin endotheli cell next generat sequenc recent improv next generat sequenc advanc research knowledg molecular cellular biolog sever studi reveal novel paradigm vascular biolog appli method model vascular develop requir optim cell isol techniqu embryon postnat tissu cell yield viabil puriti need maxim obtain accur reproduc result next generat sequenc approach neonat mous retin vascular model use research studi mechan vascular develop research use model investig mechan angiogenesi arteri venous fate specif blood vessel format matur appli next generat sequenc techniqu studi retin vascular develop model requir optim method isol retin endotheli cell maxim cell yield viabil puriti protocol describ method murin retin tissu isol digest purif use fluoresc activ cell sort fac result indic fac purifi cdcd endotheli cell popul high enrich endotheli cell gene express exhibit chang viabil min post fac includ repres result next generat sequenc approach endotheli cell isol use method includ bulk rna sequenc singl cell rna sequenc demonstr method retin endotheli cell isol compat next generat sequenc applic method retin endotheli cell isol allow advanc sequenc techniqu reveal novel mechan vascular develop

D042783;D015861

T025;T047;T046

global identif co translat interact network select ribosom profil recent year becom evid ribosom decod mrna also guid emerg polypeptid chain crowd cellular environ ribosom provid platform spatial kinet control bind membran target factor modifi enzym fold chaperon even assembl high order oligomer complex well protein protein network format step recent discov coordin synthesi describ select ribosom profil method develop captur co translat interact vivo detail various affin purif step requir captur ribosom nascent chain complex togeth co translat interactor well mrna extract size exclus revers transcript deep sequenc big data analysi step requir deciph co translat interact near codon resolut

D014176;D012270

T044;T026

nerv ultrasound protocol detect dysimmun neuropathi nerv ultrasound increas use differenti diagnosi polyneuropathi complementari tool nerv conduct studi morpholog alter peripher nerv increas cross section area csa describ various immun mediat polyneuropathi promin morpholog chang nerv ultrasound describ chronic inflammatori demyelin polyneuropathi cidp spectrum diseas cidp may distinguish hereditari polyneuropathi measur extent pattern nerv swell csa increas typic find demyelin inflammatori neuropathi multifoc nerv swell inhomogen fascicular structur csa increas demyelin hereditari neuropathi occur general homogen manner non inflammatori axon neuropathi nerv appear normal slight csa increas especi typic entrap site articl present technic requir nerv ultrasound examin procedur use standard examin protocol current refer valu csa typic sonograph patholog find patient inflammatori neuropathi

D020277

T047

prepar multifunct silk base microcapsul load dna plasmid encod rna aptam riboswitch introduc protocol prepar dna laden silk fibroin microcapsul via layer layer lbl assembl method sacrifici spheric core follow adsorpt prime layer dna plasmid format robust microcapsul facilit induc sheet silk secondari structur acut dehydr singl silk layer henc layer occur via multipl hydrogen bond hydrophob interact upon adsorpt multilay shell core shell structur function gold nanoparticl aunp andor antibodi igg use remot sens andor target deliveri adjust sever key paramet sequenti deposit key macromolecul silica core presenc polym primer concentr dna silk protein well number adsorb layer result biocompat dna laden microcapsul variabl permeabl dna load upon dissolut silica core protocol demonstr format hollow robust microcapsul dna plasmid immobil inner surfac capsul membran creat select permeabl biocompat membran dna plasmid extern environ preserv dna long term storag play import role improv output respons spatial confin plasmid activ dna templat access test vitro transcript translat reaction cell free system dna plasmid encod rna light aptam riboswitch success activ correspond analyt visual local fluoresc label rna transcript gfpa protein shell membran

D052157;D053768;D058928

T114;T073;T123

generat transgen knockout strongyloid speci microinject genus strongyloid consist multipl speci skin penetr nematod differ host rang includ strongyloid stercorali strongyloid ratti stercorali human parasit skin penetr nematod infect approxim million peopl rat parasit ratti close relat stercorali often use laboratori model stercorali stercorali ratti easili amen generat transgen knockout exogen nucleic acid deliveri techniqu intragonad microinject emerg model system parasit helminth yet amen techniqu parasit strongyloid adult inhabit small intestin host releas progeni environ via fece environ larva develop free live adult live fece produc progeni must find invad new host environment generat uniqu strongyloid speci similar enough morpholog model free live nematod caenorhabd elegan techniqu develop c elegan adapt use parasit nematod includ intragonad microinject use intragonad microinject wide varieti transgen introduc strongyloid crisprca compon also microinject creat mutant strongyloid larva techniqu intragonad microinject strongyloid includ prepar free live adult inject procedur select transgen progeni describ imag transgen strongyloid larva creat use crisprca mutagenesi includ aim paper enabl research use microinject creat transgen mutant strongyloid

D017170;D017171

T204

kinetx combin flow cytometri assay analysi softwar framework quantit measur categor platelet activ real time platelet react rapid vascular injuri undergo activ respons rang stimuli limit blood loss mani platelet function test measur endpoint respons defin time period rate platelet activ howev rate platelet convert extracellular stimuli function respons essenti factor determin effici respond injuri bind form thrombus signal recruit platelet paper describ flow cytometri base platelet function assay enabl simultan data acquisit sampl stimul util newli develop bespok open sourc softwar kinetx enabl quantit kinet measur platelet granul releas fibrinogen bind intracellular calcium flux kinetix develop r user alter paramet degre smooth identif out data point time scale aid user unfamiliar r environ kinetix analysi data perform singl command togeth allow real time platelet activ metric rate acceler time peak rate time peak calcium qualit shape chang accur reproduc measur categor kinet measur platelet activ give uniqu insight platelet behavior first stage activ may provid method predict recruit platelet form thrombus

D001792;D015539

T025;T042

prepar rear axen insect tissu cultur seedl host gut microbiota interact studi leaf beetl insect gut colon divers bacteria profound impact host physiolog trait introduc particular bacteri strain axen insect power method verifi gut microbi function elucid mechan under gut microb host interact administ antibiot steril egg surfac two common use method remov gut bacteria insect howev addit potenti advers effect antibiot insect previous studi indic feed antibiot could elimin gut bacteria thus germ free artifici diet general employ maintain axen insect tedious labor intens process fulli resembl nutrit compon natur food describ effici simpl protocol prepar maintain axen larva leaf beetl plagiodera versicolora specif surfac beetl egg steril follow germ free poplar leav use rear axen larva axen status insect confirm via cultur depend cultur independ assay collect combin egg disinfect germ free cultiv effici conveni method develop obtain axen p versicolora provid readili transfer tool leaf eat insect

D001517;D000069196

T204;T007;T001

cryo section dissect adult subependym zone accur deep quantit proteom analysi subependym neurogen nich consist paraventricular ribbon later ventricular wall later ventricl subependym zone sez thin distinct region expos ventricl cerebrospin fluid isol nich allow analysi neurogen stem cell microenviron howev extract small tissu proteom analysi challeng especi mainten consider measur depth achiev reliabl robust new method term cryo section dissect csd combin high precis minim tissu perturb develop address challeng method compat state art mass spectrometri ms method allow detect low abund nich regul studi compar csd proteom data method data obtain laser captur microdissect lcm standard wholemount dissect csd method result twice quantif depth less half prepar time compar lcm simultan clear outperform dissect precis wholemount dissect henc csd superior method collect sez proteom analysi

D020547;D020543

T116;T023;T030;T123

chemic modif tryptophan residu recombin ca atpas n domain studi tryptophan an fret sarcoendoplasm reticulum ca atpas serca p type atpas crystal various conform detail function inform may nonetheless obtain isol recombin domain engin trpleu tyrtrp recombin nucleotid bind domain n domain display fluoresc quench upon ligand bind extrins fluorophor name anilino naphthalen sulfon an bind nucleotid bind site via electrostat hydrophob interact arg ala leu phe residu an bind evidenc increas fluoresc intens excit wavelength nm howev excit nm increas fluoresc intens seem coupl quench n domain intrins fluoresc fluoresc spectra display fster reson energi transfer fret like pattern therebi suggest presenc trp an fret pair appear support short distanc tyrtrp an studi describ analysi trp an fret pair trp chemic modif fluoresc quench mediat n bromosuccinimid nbs chemic modifi n domain an fluoresc increas excit nm similar excit nm henc nbs mediat chemic modif trp residu use probe absenc fret trp an absenc trp fluoresc one observ increas an fluoresc chemic modif trp residu protein nbs may use examin fret trp residu close bound an assay like also use use fluorophor

D002118;D014364

T116;T121;T109;T123;T046;T196

free float immunostain mous brain immunohistochem stain mous brain routin techniqu common use neurosci investig central mechan under regul energi metabol neurobiolog process howev qualiti reliabl reproduc brain histolog result may vari among laboratori stain experi necessari optim key procedur base differ speci tissu target protein work condit reagent paper demonstr reliabl workflow detail includ intra aortic perfus brain section free float immunostain tissu mount imag follow easili research field also discuss modifi procedur satisfi individu need research illustr reliabl effici protocol perineuron net stain biotin label wisteria florbunda agglutinin wfa arginin vasopressin avp anti avp antibodi mous brain final critic detail entir procedur address advantag protocol compar protocol taken togeth paper present optim protocol free float immunostain mous brain tissu follow protocol make process easier junior senior scientist improv qualiti reliabl reproduc immunostain studi

D001921;D005109

T023;T024

sampl prepar comput tomographi base three dimension visual murin hind limb vessel blood vessel complex network tree like structur vascular network essenti maintain circul maintain organ function clarifi mechan blood vessel format therefor extrem use elucid development process patholog mechan murin hind limb vessel often use model physiolog patholog angiogenesi evalu main perform via two dimension method use tissu section howev method evalu three dimension vascular morpholog particular limit paper introduc method visual murin hind limb use comput tomographi ct radiat opaqu resin inject descend aorta whole vessel fill dye adjust time dye inject arteri specif fill also possibl sampl obtain micro x ray ct devic contrast method provid basic techniqu evalu murin blood vessel lower extrem furthermor method use visual blood vessel diaphragm evalu blood vessel abdomin organ

D021621;D014057

T062;T073;T060

stereotax viral inject gradient index len implant deep brain vivo calcium imag miniatur fluoresc microscop miniscop potent tool vivo calcium imag freeli behav anim offer sever advantag convent multi photon calcium imag system compact light weight afford allow record freeli behav anim protocol describ brain surgeri deep brain vivo calcium imag use custom develop miniscop record system prepar procedur consist three step includ stereotax inject virus desir brain region mous brain label specif subgroup neuron genet encod calcium sensor implant gradient index grin len relay calcium imag deep brain region miniscop system affix miniscop holder mous skull miniscop attach later perform vivo calcium imag miniscop fasten onto holder neuron calcium imag collect along simultan behavior record present surgeri protocol compat commerci custom built singl photon two photon imag system deep brain vivo calcium imag

D002118;D007909

T121;T073;T196;T123

comparison singl dose versus multidos metronidazol select clinic factor treatment trichomona vaginali women random control trial g singl dose metronidazol mtz versus mg twice daili day multidos trichomona vaginali treatment multidos superior examin effect similar select clinic factor determin treatment recommend could target

D014247;D014246;D016585

T204;T047

limit risk compens among women inject drug result project sexual health equiti preexposur prophylaxi demonstr studi philadelphia impact preexposur prophylaxi uptak sexual inject relat behavior among women inject drug poor understood week preexposur prophylaxi uptak among women inject drug associ increas share inject equip syring chang condomless sex provid limit evid risk compens vulner popul

D015658;D000069576;D065129;D015819

T080;T048;T047;T061

predict emerg primari secondari syphili among women reproduct age us counti syphili sexual transmit infect caus sever congenit diseas treat pregnanc rise unit state object identifi us counti elev risk emerg primari secondari p syphili among women reproduct age

D012749;D013587

T047

test sexual transmit infect use wet dri self collect brush sampl among women mombasa kenya compar detect chlamydia trachomati neisseria gonorrhoea trichomona vaginali use dri wet self collect sampl use brush among femal engag sex work mombasa kenya detect vaginali n gonorrhoea dri wet sampl similar c trachomati detect dri sampl appear lower

D002690;D006069;D012749;D014246

T204;T047