Claim: The importation of proteins containing a nuclear localization signal requires a heterodimer of importin alpha, which binds the nuclear localization signal-containing cargo in the cytoplasm.
Evidence: Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. The import of proteins containing a classical nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits. A nuclear localization signal or sequence (NLS) is an amino acid sequence that 'tags' a protein for import into the cell nucleus by nuclear transport.