Claim: ARAF has amino acid substitutions that could be responsible for its low basal activity.
Evidence: In addition, the A-Raf protein has amino acid substitutions in a negatively charged region upstream of the kinase domain (N-region). Serine/threonine-protein kinase A-Raf or simply A-Raf is an enzyme that in humans is encoded by the ARAF gene. This could be responsible for its low basal activity.