Claim: The Benesi–Hildebrand method can be used to observe one-to-one binding between a single host and guest using UV/Vis absorbance with the assumption that the initial saturation of the guest is much larger than the initial concentration of the host, then the absorbance from H0 should be negligible.
Evidence: With the assumption that the initial concentration of the guest (G0) is much larger than the initial concentration of the host (H0), then the absorbance from H0 should be negligible.