Claim: Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding, which is an attractive interaction between two molecules, and includes target molecules such as enzymes, antigen, receptor and biotin.
Evidence: Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between the biomolecule and another substance. Molecular binding is an attractive interaction between two molecules that results in a stable association in which the molecules are in close proximity to each other.It is formed when atoms or molecules bind together by sharing of electrons. Enzymes Antigen Receptor Biotin/Biotin-conjugated molecule Typical Biological Interactions Used in Affinity Chromatography