Please select the most similar patent number from A, B, C and D. Which number is?
Disclosed is a mutant Cry2Aa insecticidal protein. Based on the amino acid sequence shown in SEQ ID NO. 1, the amino acid sequence of the insecticidal protein has one, two, or three mutations as follows: A) W78F; B) L144A, and C) R213E. Preferably, D) 28 amino acids are deleted at the N-terminal of the amino acid sequence. Further disclosed is a gene encoding the mutant Cry2Aa insecticidal protein, a recombinant expression vector comprising the same, and a method for improving the insect resistance of a plant using the same.
A: Provided are a proline hydroxylase and an application thereof. The proline hydroxylase includes (a) a protein having the amino acid sequence as shown in SEQ ID NO: 2; (b) a protein in which the amino acid sequence as shown in SEQ ID NO: 2 has gone through mutations of one or more amino acids and which has proline hydroxylase activity; or (c) a protein which retains the mutations of one or more amino acids as in (b), which has having has proline hydroxylase activity and which has at least 78% homology with the amino acid sequence of the protein in (b). Proteins having the amino acid sequence as shown in SEQ ID NO: 2 and mutants obtained by genetically engineering the same have higher catalytic specificity or significantly increased catalytic activity when compared to existing proline hydroxylases.
B: Provided is a bacterium for producing L-amino acid. The bacterium has improved expression of a polynucleotide for encoding a protein represented by SEQ ID NO:3 and improved expression of a polynucleotide for encoding a protein represented by SEQ ID NO:31, and/or has mutations in bases at positions -45bp and -47bp of a promotor region represented by SEQ ID NO:57. Also provided are a polynucleotide, proteins encoded thereby, a recombinant carrier and a recombinant strain comprising a polynucleotide sequence, and a method for producing L-amino acid. The polynucleotide encodes a protein which is represented by SEQ ID NO:3 and has arginine at position 334 substituted by a terminator or encodes a protein which is represented by SEQ ID NO:31 and has tyrosine at position 592 substituted by phenylalanine, or is formed by mutations in bases at positions -45bp and -47bp of a promotor region represented by SEQ ID NO:57.
C: Disclosed are a mutant protoporphyrinogen oxidase in tobacco mitochondria and a coding gene and a use thereof. The mutant is a protein derived from SEQ ID NO: 1 by subjecting, a non-polar amino acid of at least one position of position 353, position 356 and position 372 in an amino acid sequence depicted by SEQ ID NO: 1, to substitution, deletion or addition of one or more amino acids without altering enzyme activity. Encoding the mutant gene into a target plant enables the target plant to acquire a resistance to a PPO enzyme inhibitor (herbicide) without overly affecting a catalytic activity of the enzyme. Therefore, the mutant and the coding gene thereof are useful for cultivating a plant with herbicide resistance.
D: The present invention relates to obtaining Sip1Aa soluble pesticidal protein mutants, named Sip153-248 and Sip158-243 protein, which falls within the field of biotechnology. According to the invention, the amino acid sequence of the Sip1Aa protein is changed at two amino acids, respectively, the pesticidal activity of the obtained mutant protein Sip153-248 is improved by 2.76 times, and the pesticidal activity of Sip158-243 is improved by 2.26 times, and the solubility and stability thereof are improved to a certain extent, thereby effectively overcoming the problem of finding high virulence genes of the Bacillus thuringiensis expressed protein to colaphellus bowringi baly, and delaying the resistance development of pests to engineered bacteria and transgenic plants.
Answer: D