Please select the most similar patent number from A, B, C and D. Which number is?
Provided is a method for extracting plasmid DNA in bacteria, realizing lysis and neutralization during plasmid production in two mixing assemblies connected in series, and comprising the following steps: (1) mixing, (2) lysing, and (3) neutralizing. Step (1) is completed in a first mixing assembly; step (2) is completed in a lysis helical tube; step (3) is completed in a second mixing assembly; and the first mixing assembly, the lysis helical tube and the second mixing assembly are sequentially connected in series. A device used in the plasmid preparation process is simple, is convenient to operate, is low in costs, can remove a large amount of impurities during cell lysis without professional customized device and expensive device, has safe components, realizes automatic continuous lysis, and facilitates industrial production.
A: The present invention relates to an optimal production method for a graphene powder material having a three-dimensional structure, comprising the following steps: (1) sensitizing a polymer, wherein sensitizing treatment comprises sequentially performing alkalization treatment, acidification treatment, metal ion exchange and pore-forming treatment on the source material; (2) drying the sensitized raw material obtained in step (1); (3) performing high-temperature pyrolysis treatment on the dried raw material obtained in step (2); (4) in a solvent, performing disintegration treatment on the sample following the high-temperature pyrolysis treatment in step (3); (5) performing metal recovery treatment on the sample following disintegration in step (4). The whole process for preparing the graphene powder material having a three-dimensional structure according to the method of the present invention does not require grinding and pulverization, thus optimizing cumbersome preparation processing when preparing graphene according to a conventional catalytic cracking method, simplifying the production procedure and reducing production costs.
B: The present invention relates to a crude oil cracking method and system. The method comprises: delivering crude oil to a first tube group of a convection section of a cracking furnace for preheating and then performing vaporization to obtain a first gas phase and a first liquid phase; performing high-pressure extraction on the first liquid phase to obtain a non-asphalt oil and asphalt; and mixing the first gas phase and the non-asphalt oil with water vapor separately, or mixing the first gas phase with the non-asphalt oil first and then mixing the mixture with water vapor, then delivering the same to a second tube group of the convection section of the cracking furnace for heating, then delivering same to a radiation section of the cracking furnace for cracking to obtain a cracking product, and separating the cracking product to obtain a low-carbon olefin. In the present invention, after crude oil is preheated and vaporized in a cracking furnace, an obtained gas phase enters the cracking furnace, and an obtained liquid phase passes through a supercritical extraction unit to obtain a separated extract oil and the extract oil enters the cracking furnace; the method can effectively improve the crude oil cracking efficiency, improve the yield of a low-carbon olefin in the product, extend the operating cycle, and reduce coking of a gasified crude oil mixture in a cracking process.
C: Provided is a mixing module (100), comprising a housing (1), multiple partitions (2), and a mixing device (3). The multiple partitions (2) comprise a first partition (21) and a second partition (22) spaced apart from each other. The first partition (21) and the second partition (22) separate the housing (1) into a first chamber (41), a second chamber (42), and a third chamber (43). The mixing device (3) is at least partially supported by the first partition (21), and comprises a first mixing pipe (31) and a second mixing pipe (32) parallel to the first mixing pipe (31). The first mixing pipe (31) comprises multiple first swirl plates (312). The second mixing pipe (32) comprises multiple second swirl plates (322). Both the first swirl plates (312) and the second swirl plates (322) are located in the first chamber (41). The mixing module (100) further comprises a first air deflector (35) and a second air deflector (36) that are secured on the second partition (22) to force air to flow in reverse. The mixing module (100) increases the distance and duration of urea evaporation and improves uniformity of air flow mixing.
D: A chaotropic agent and a method for extracting genomic DNA by using same. The chaotropic agent contains sodium bromate, and the compound can quickly and effectively separate histone in a nucleosomal structure from genomic DNA. The method for extracting genomic DNA by using the chaotropic agent comprises the following steps: adding a cell lysis solution to a sample to rupture cells; then mixing the sample with a chaotropic agent uniformly to obtain a material in which histone and genomic DNA are separated; adding protein extracting liquid, followed by uniform mixing and centrifugation, to obtain supernatant after protein is extracted; and mixing the supernatant with a DNA precipitant, and centrifuging the mixture to obtain a DNA precipitate. Compared with a method using sodium perchlorate, this method is different in the type of the chaotropic agent, and has lower valence and more appropriate yield and purity of the genomic DNA. Compared with a conventional proteinase K method, this method requires no long-time digestion, saves a great deal of time, overcomes the defect of susceptibility to change in protease K activity and the disadvantage of inadequate digestion, and features good repeatability and high stability.
Answer: D