Please select the most similar patent number from A, B, C and D. Which number is?
The invention belongs to the field of collagen/gelatine preparation, and provides a method for the preparation of soluble collagen. The invention employs a dual 90 bone gelatine prepared by alkaline process, adds acid protease to control the degradation of bone gelatine after adjusting the pH of the dual 90 bone gelatine solution by inorganic acid; or adds neutral protease or alkaline protease to control the degradation of bone gelatine after adjusting the pH by inorganic base; brings the bone gelatine solution with protease added to react at room temperature, then adjusts the pH of the solution after reaction to 5-6.5; then performs a heat extraction by increasing the temperature to 65-75 ℃; inactivates the enzyme by water bath at 95-100 ℃ for 5-15 minutes; performs purification and drying by ultrafiltration and spray drying; and eventually gives a soluble collagen powder product. The invention also provides a soluble collagen obtained by the method, and use of the soluble collagen in the preparation of pharmaceuticals, foods and cosmetics.
A: A quality control method for biologically active collagen. Specifically, acidic zymolytic conditions are employed to achieve efficient extraction of bovine tendon type-I collagen and optimize collagen production processes. Enzyme reaction time, dialysis time, and dialysis bag retained molecular weight are changed to control the molecular weight and molecular weight distribution of the collagen. A terminal peptide is removed to control the immunogenicity of the collagen. Transmission electron microscopy is used to characterize the triple helix structure of the collagen to determine the biological activity of the collagen. The prepared type-I active collagen meets international standards, providing a basis for further application of collagen.
B: Disclosed is a method for preparing packaged water, the method comprising the following steps: step 1. mixing sodium alginate, konjac flour, xanthan gum, carrageenan and potassium sorbate until uniform, adding same to water, and then stirring same until uniform under a condition of 90ºC to obtain a mixed liquid; step 2. mixing gelatin and water and then placing same in an ice water bath for 4 hours, then heating same to 120ºC and then adding a mixed material, and mixing same until uniform to obtain a wall material solution, wherein the mixed material is prepared by dissolving soluble proteins in water; and step 3. adding the wall material solution into a mold, reducing the temperature to 50ºC to form a layer of film, then bringing one side of the film into contact with a calcified material, and then making the other side of the film into a capsule for containing drinking water, charging the drinking water into the capsule, and sealing same to obtain the packaged water. The packaged water can be edible as a whole, thereby avoiding creating plastic wastes.
C: The present invention relates to the technical field of biological food materials, and disclosed in the present invention are a cross-linked hydrogel for muscle stem cell culture, and a preparation method therefor and an application thereof. The present invention comprises: dissolving collagen to form a solution, then adding certain amounts of an alginate and a heparan sulfate proteoglycan, and uniformly mixing same with the collagen solution; and then adding ε-PL and TG enzyme into the solution, uniformly stirring same, and placing the slurry in a mold for crosslinking to obtain a hydrogel. According to the hydrogel in the present invention, the collagen is linked to the polylysine and heparan sulfate proteoglycan by means of the TG enzyme to form covalent cross-linking, and a dense three-dimensional &#34;egg box&#34; network structure is formed by means of physical electrostatic interaction between the polylysine and alginate. The use of the hydrogel can enhance absorption of nutrients by muscle stem cells and facilitate the growth of the muscle stem cells. The double-cross-linked network hydrogel has the potential as scaffolds for growth of muscle stem cells in stem cell-based meat culture.
D: Disclosed are a pre-loadable dried biological heart valve and a preparation method thereof. The preparation method comprises: Step A: soaking a fresh animal pericardium in an aqueous solution of soluble elastin or glycosaminoglycan, followed by subjecting the pericardium to a first cross-linking reaction in a mixed solution of carbodiimide/N-hydroxysuccinimide to allow the soluble elastin or glycosaminoglycan to bind to the pericardium via a chemical bond; and Step B: subjecting the pericardium having undergone the first cross-linking to a second cross-linking reaction in an aqueous glutaraldehyde solution, followed by drying the pericardium having undergone the second cross-linking, thus obtaining the pre-loadable dried biological heart valve. The dried biological heart valve obtained by the above preparation method has good toughness, and can rapidly flatten out in a simulated bending test.
Answer: A