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multidomain-measextract-corpus

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This study was approved by the Institutional Review Board and Ethics Committees of the University of California, San Francisco, and written informed consent was obtained in all cases. The patient with the PGRN S116X mutation followed the classic clinical progression for FTD and developed parkinsonism, as do all FTD patients with PGRN mutations, but he did not show typical features of PD dementia. The patient with sporadic FTD also showed parkinsonism. Skin biopsies were collected, cut into small pieces, and placed on culture dishes to allow the fibroblasts to expand. The cells were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 1X nonessential amino acids, and penicillin/streptomycin (100 U/ml). iPSCs were generated as described previously (Takahashi et al., 2007). Please see Supplemental Information for more details.

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S2211124712002884-1060

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

iPSC colonies were detached with accutase (Millipore) and grown as embryoid bodies (EBs) in suspension for 5–6 days in iPSC medium without basic fibroblast growth factor. EBs were allowed to attach and form rosettes. Ten-day-old rosettes were collected and grown in suspension as neurospheres. Neurospheres were dissociated after 3–4 weeks, and the cells were plated on glass coverslips (BD Biosciences) or plates coated with poly-D-lysine (0.1 mg/ml) and laminin (10 μg/m). Neurons were used after 2-4 weeks in culture.

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S2211124712002884-1110

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

(F) Intracellular PGRN levels in iPSC-derived neurons after medium collection. Values of control line 20 were set to 100% (n = 3–4 independent cultures).

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S2211124712002884-649

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

(C) Control lines 16 and 17, sporadic lines 12 and 23, and PGRN S116X lines 1 and 14 were immunostained for AFP (endoderm), desmin (mesoderm), and βIII-tubulin (ectoderm), and counterstained with DAPI (nuclei). All lines showed a normal karyotype. Scale bar: 50 μm.

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S2211124712002884-682

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

(D) PGRN secreted into the medium over 24 hr of microglia derived from control, sporadic, and PGRN S116X iPSCs. The average of values for control lines was set to 100%. Values are mean ± SEM.

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S2211124712002884-705

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

The two FTD patients under investigation in this study were part of a longitudinal dementia research program at the Memory and Aging Center, University of California, San Francisco. Both had an 8-year history of behavioral changes and memory impairment at the time of tissue collection for this study. One patient, a 67-year-old male with sporadic FTD, tested negative for mutations in GRN, MAPT, and C9ORF72. The other patient, a 64-year-old male with a significant family history of dementia, had behavioral variant FTD. MRI in this patient demonstrated severe bifrontal and temporal atrophy associated with gliosis in the frontal lobes (greater on the right). One year later, MRI scans showed progression of atrophy and gliosis. Genetic testing revealed a novel nonsense mutation in GRN, p.S116X (g.4627C > A, c.347C > A), which is predicted to result in a premature stop codon. Both FTD patients had parkinsonism, which is typical of all FTD patients with PGRN mutations. An age-matched subject, a clinically normal 64-year-old male with no mutations in GRN, MAPT, or C9ORF72, served as a control.

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S2211124712002884-903

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

To assess the in vivo properties of hESC-derived trigeminal placode precursors, PIP-induced neuronal clusters, derived from a constitutively GFP-positive hESC line (Figures S5A and S5B), were injected into the developing chick embryo targeting the early trigeminal anlage at H&H stage 10–12 (Figure S5C). Human cells were identified based on GFP expression and use of human specific antibodies against cytoplasmic antigen (hCA). Two days after in ovo transplantation, surviving GFP+ cells were found dispersed in the area of the endogenous chick trigeminal ganglion (Figure 4P). We observed extensive GFP+ human fiber bundles coexpressing hCA and peripherin (Figures 4Q and 4R). In contrast, no hCA or peripherin expression was detected in the neural tube of the embryo (Figure S5D). The in vivo fiber outgrowth 2 days after transplantation was reminiscent of the extensive in vitro fiber outgrowth of replated trigeminal neuron clusters (Figure S5A). Peripherin expression in vivo (Figure 4S) confirmed the peripheral neuron identity of the grafted cells.

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S2211124713006475-1195

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

We next addressed whether hESC-derived trigeminal neurons can engraft in the adult mouse CNS and project toward their physiological target. The trigeminal nuclei in the brainstem receive afferent innervation from the trigeminal sensory ganglion that is relayed to the contralateral thalamus. The pons was selected as site for transplantation, because it is surgically accessible and located within proximity of the trigeminal brain stem nuclei that receive afferent input from the trigeminal ganglia. Hence, GFP+ human trigeminal neuron clusters were injected into adult NOD/SCID mice via stereotactic surgery (see Experimental Procedures). Histological analysis 4 weeks after transplantation showed survival of GFP+ human cell graft in the ventral pons (Figure S5E). Although GFP+ cell bodies remained tightly clustered at injection site, GFP+ fibers showed extensive projections into the host brain (n = 6) including the endogenous trigeminal nuclei (Figure S5F). Expression of BRN3A confirmed the sensory neuron identity of the cells (Figure S5G). Graft-derived human fiber bundles (hNCAM+ and GFP+) were observed emanating from the graft core (Figure S5H). These data demonstrate in vivo survival of trigeminal placode derivatives, differentiation along sensory neuron lineage, and the establishment of axonal projections toward relevant endogenous targets in the embryonic chick and adult mouse brain.

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S2211124713006475-1205

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

(O) GH plasma levels using a human specific ELISA (6 weeks after transplantation).

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S2211124713006475-841

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

The second feature of interest in the network change is that it is observed in the early stages of auditory processing. The MMNm component is an early (between 100 and 200 ms), automatic or largely pre-attentive response (Naatanen et al., 2007). Particularly relevant is that a prefrontal contribution to change detection also occurs within this early time window, demonstrated in Garrido and colleagues' dynamic causal modelling of a roving oddball paradigm (Garrido et al., 2008) and the effects of frontal lesions (Alho et al., 1994). This suggests that if the prefrontal cortical dysfunction in the two patient groups is the cause of the altered network response, then this affects the very early stages of stimuli processing. Although this early component is distinct from the later M300 response, which reflects attentional processing (Wronka et al., 2008), it is still relevant to high order cognitive processes, (Naatanen et al., 2007, 2012).

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S2213158213000302-1597

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

For group analyses, the following procedures were applied. (1) T1-weighted sMRIs from each subject (Fig. 1A) were registered to MNI space (Montreal Neurological Institute, MNI-152 atlas as in Fig. 1B) using an affine transformation (FLIRT–FMRIB's Linear Image Registration Tool) (Jenkinson and Smith, 2001) in FSL (www.fmrib.ox.ac.uk/fsl/). (2) The cortical (Fig. 1C) and subcortical masks with pre-defined brain regions from the standard atlas were transferred to the individual's headspace (Fig. 1D), using the inverse of the transformation obtained in the first step: the Harvard-Oxford Atlas, part of the FSL software with masks of 96 cortical gray-matter regions (48 regions in each hemisphere), 21 sub-cortical regions, and cerebellum, was used. (3) The regional masks were down-sampled to a cubic source grid with voxels of 5 mm per side (Fig. 1E). (4) VESTAL MEG source imaging used the source grid from step 3. This step permits group-based analyses. In the shown example, MEG responses evoked by S1 localized to left and right Heschl's gyri (Fig. 1F). (5) Finally, for regions of interest (ROIs), the source time course was obtained by summing activity from all ROI voxels. Fig. 1H shows the time course from left Heschl's gyrus (dark blue region in Fig. 1C and D).

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S2213158213000582-1327

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

Prior to VESTAL analyses, a 5–55 Hz bandpass filter was applied. VESTAL analyses examined activity 30–130 ms post-stimulus producing a 4D activation map (3D volumes across time) as well as a 2D source time-course matrix. The average percent variance explained for gradiometer data using VESTAL was 95.81% for HC and 94.38% for SZ. The average percent variance explained for magnetometer data using VESTAL program was 96.24% for HC and 93.17% for SZ. There were no group differences in percent variance explained for gradiometer data (t(39) = 1.16, p = 0.25) or magnetometer data (t(39) = 1.31, p = 0.20).

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S2213158213000582-1340

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

(I–M) Teratoma formation at 3 months after transplantation in the testes of SCID mice. H&E staining of the sections showed histological features of the neuroepithelium (J), cartilage (K), muscle (L) and gut-like epithelium (M).

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S2213671113000738-430

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

(R) Quantification of immunocytochemical analyses for each iPSC line. Data are shown as the means ± SD (n = 3 independent experiments). SER, serotonin, TUBβIII, β-tubulin class III. Scale bars: 200 μm in (A)–(H), 50 μm in insets of (C)–(F), 100 μm in (J)–(M) and (Q).

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S2213671113000738-435

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

(A) Flow-cytometric analyses for MHC-I (HLA-A, HLA-B, and HLA-C). Incubation of the cells with IFN-γ for 48 hr increased the MHC-I expression (green).

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S2213671113000738-445

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

(Q–S) Magnetic resonance images of a representative animal (No. 6, autograft) at 3 months after the transplant. The arrowheads indicate the directions of the cell injections. (Q) coronal, (R) axial, and (S) sagittal. The letter L indicates the left side.

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S2213671113000738-485

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

For the first two animals (Nos. 1 and 4), we established iPSCs from fibroblasts derived from the oral mucosa using retroviral vectors (Okita et al., 2011). For the other two animals (Nos. 6 and 8), we used peripheral blood mononuclear cells (PBMCs) with nonintegrating episomal vectors (Okita et al., 2013). We selected the best clone from each animal according to the following criteria: a stable embryonic stem cell (ESC)-like morphology of the colonies after passaging, expression of pluripotent markers, few or no integrated transgenes (Figures 1A–1F; Figure S1 available online), and the potential for stable neural differentiation. A PCR analysis revealed that all of the clones with retroviral vectors showed apparent expression of remaining transgenes (Figures S1C and S1D), whereas the clones with episomal vectors never did (Figure S1F). To detect the iPSC-derived cells in a brain, we introduced GFP (Figures 1G and 1H). The selected clones of iPSCs had the potential to generate teratomas in the testes of a severe combined immunodeficiency (SCID) mouse within 12 weeks (Figures 1I–1M).

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S2213671113000738-647

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

In sequential PET studies, we observed increased uptake of [11C]PK11195 in one allograft (animal No. 10) at 3 months (Figures 3A and 3B). We could not detect any apparent uptake in the other animals or at any other time points (Figure S2). Intriguingly, the serum level of IFN-γ temporarily increased at 2 months after the transplant in three animals (Figure 3C). An immunofluorescence study conducted at 3.5–4 months showed that MHC-II+ cells were more frequently found in allografts than in autografts, especially in the monkey with increased uptake of [11C]PK11195 (Figure 3D, No. 10). The MHC-II staining never overlapped with that of GFP of the donor cells (Figure 3F), whereas it generally overlapped with that of IBA1 (Figure 3G), indicating that MHC-II was expressed by host-derived microglia. Consistently, the number and density of IBA1+ cells were higher in allografts than in autografts (Figures 3E, 3H, and S4C). An increase in the expression of MHC might trigger the recruitment of circulating immune cells, including T cells. An immunofluorescence study revealed that more CD45+ cells (a marker for pan-leukocytes) accumulated in allografts compared with autografts (Figures 3I and 3J). Most of the CD45+ cells were CD3+ T cells, and 60% of them were CD8+ killer T cells (Figures 3K and 3L). These findings suggest that an acquired immune response was elicited only in the allografts in the primate brain.

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S2213671113000738-684

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

Another important finding is that, in spite of the immune responses mounted by the host brain, a substantial number of TH+ cells survived in the allografts. This is consistent with previous clinical reports of human fetal cell transplantation. Postmortem analyses of the patients revealed robust survival of DA neurons in spite of the fact that numerous immune cells were present around the graft (Kordower et al., 1997). In two double-blind clinical trials, immunosuppressive drugs were never used (Freed et al., 2001) or were withdrawn after 6 months (Olanow et al., 2003). In these cases, the cells from multiple fetuses were used without HLA matching, but more than 50,000 TH+ cells had survived after several years. Our quantitative PCR (qPCR) study in vitro showed that the expression of MHC-I increased in response to IFN-γ, but the expression level was still 1/10 that of untreated monkey peripheral blood cells (Figure 2B). The in vivo studies revealed that the serum level of IFN-γ increased at 2 months, and CD45+ cells (including CD8+ cells) accumulated in the allografts 3.5–4 months after the transplant. On the other hand, the levels of INF-γ in the cerebrospinal fluid (CSF) and the levels of tumor necrosis factor α (TNF-α) in both the serum and CSF were below the limit of detection by ELISA (data not shown). An immunofluorescence study did not reveal any apparent expression of MHC-I by the grafted cells (Figure S4A). Therefore, it is possible that the immune response in the primate brain was not strong enough to reject all of the donor cells. These findings closely correlate with the results of previous murine experiments (Hudson et al., 1994; Shinoda et al., 1995). To apply our findings to a more clinically relevant setting, we investigated the expression of HLA-I during neural differentiation of human ESCs (hESCs) and iPSCs by qPCR (Figure S4D). The expression level was 1/100 compared with that of human peripheral blood cells in both hESCs and iPSCs, and it was similarly elevated in response to IFN-γ. It is difficult to precisely compare immunogenicity in monkeys with that in humans, but the low expression level of MHC-I by the donor cells may account for the mild rejection in both monkey and human neural transplantation.

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S2213671113000738-738

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

(C) C-peptide and PDX1 expression was confirmed by immunocytochemistry of cells differentiated for 25 days.

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S2213671113000908-640

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

Our group has developed a defined culture system to direct the differentiation of hPSCs into a near-homogenous population of definitive endoderm (DE) cells that have the capacity to differentiate into hepatocytes and pancreatic progenitors (Brown et al., 2011; Cho et al., 2012; Rashid et al., 2010; Touboul et al., 2010; Vallier et al., 2009a; Yusa et al., 2011). Cells grown in these culture conditions successively express primitive streak markers (T and Mixl1), downregulate pluripotency markers (NANOG, SOX2, and POU5F1) and progressively upregulate definitive endoderm markers (CXCR4, FOXA2, GATA4, CERB, and SOX17) (Figures S1A–S1C available online). Flow cytometry analyses showed that 80% of the resulting DE population coexpresses CXCR4 and SOX17 (Figure S1D). Interestingly, the resulting population of DE cells is negative for genes marking the foregut (SOX2), the midgut/hindgut (CDX2), the pancreas (PDX1), the liver (AFP), and the lungs (HOXA1) (Figures S1E and S1F) This confirms that DE cells generated in vitro could correspond to early endoderm progenitor cells prior to anteroposterior patterning or organogenesis.

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S2213671113000908-810

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

GFP-expressing H9, BBHX8, and A1ATD-1 cells were generated by stable transfection using lipofectamine 2000 (Invitrogen) as described previously (Vallier et al., 2001). GFP-positive cells were differentiated into foregut cells and then dissociated into single cells. An individually isolated GFP cell was then transferred into a well containing non-GFP-positive hFSCs. Wells were visually inspected 12 hr after plating, and wells containing a single GFP-positive hFSC were selected for clonal expansion.

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S2213671113000908-979

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

Based on several previous publications (Khan et al., 2010, 2011), we explored the utility of adeno-associated virus (AAV) as a method to improve gene targeting efficiencies in hPSCs. For SOX2, a gene that is highly expressed in undifferentiated hPSCs, gene targeting rates were greater than 70%. Similar targeting efficiencies in hPSCs using AAV have been reported by others (Asuri et al., 2012; Khan et al., 2010, 2011; Smith-Arica et al., 2003), indicating that AAV offers a highly efficient and robust approach to target genes for HR in hPSCs.

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S2213671113000921-1279

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

(D) IF analysis of GFP and SOX2 showed colocalization in NPCs (scale bar represents 100 μm).

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S2213671113000921-714

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

Using a recombinant adeno-associated viral (rAAV)-based gene-targeting method, we inserted the gene-encoding GFP into the SOX2 locus in H9 hESCs (Figure 1A). Proper homologous recombination led to the replacement of the SOX2 open reading frame with that of GFP and a neomycin selection cassette (SV40-Neo). After infection with rAAV and G418 drug selection, a total of 36 clones were expanded and screened by Southern blotting for homologous recombination events. Among these clones, 26 (72%) were found to carry the GFP-Neo cassette in the SOX2 locus (Figure S1A available online). No clones in which both SOX2 alleles were disrupted were isolated. Our subsequent analysis focused on one of these clones, clone 23 (hSOX2-23). We confirmed appropriate gene targeting in this clone using multiple restriction digests followed by Southern blotting (Figures 1B, S1B, and S1C). We did not observe nontargeted insertions of the rAAV sequences, and cells exhibited a normal karyotype (data not shown). Flow cytometry of hSOX2-23 revealed that the majority of the cells expressed GFP (Figure 1C). By comparison, a drug-selected clone, hSOX2-25, which was negative for targeted insertion (Figure S1A), showed no detectable GFP (Figure S2A). Despite only having one copy of SOX2, hSOX2-23 had similar levels of SOX2, OCT4, and NANOG expression as hSOX2-25 and wild-type (WT) hESCs (Figure S2B). Moreover, the percentage of GFP-positive (GFP+) cells in hSOX2-23 was constant over more than 20 passages. Immunofluorescence (IF) staining of hSOX2-23 showed that 100% of GFP+ cells expressed SOX2 protein (Figure S2C). Additionally, hSOX2-23 colonies had characteristic hESC morphology (Figure S2D) and expressed markers of the undifferentiated state, such as NANOG (Figure S2E). These results show that this rAAV-based gene-targeting method can be used to efficiently disrupt genes by homologous recombination. In addition, the SOX2-GFP hESC marker line can be used to monitor SOX2 expression in undifferentiated hESCs.

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S2213671113000921-994

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

•Adult hRPESC-derived RPE had comparable in vitro characteristics to fetal hRPE•hRPE monolayers survived 4 weeks on PET carriers under the rabbit retina•Better xenograft survival may be due to the maintained hRPE cell polarity•Atrophy of the retina overlaying the hRPE xenograft remains a future challenge

train

S2213671113001306-1286

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

We asked whether encapsulating the graft in additional materials would help the surgical delivery and potentially reduce the retinal thinning. Temporary graft encapsulation with thermosensitive gelatin and/or plasmin-assisted vitrectomy, however, was found to cause more aggressive retinal destruction and choroidal engorgement on SD-OCT compared to unaided implantation of fetal hRPE/PET implants (Figure S2). In contrast, we found that all these negative effects were ameliorated through 1 to 2 mg intravitreal triamcinolone (TCA) injection at the end of the surgery. This long-acting synthetic corticosteroid is routinely given intraocularly to reduce immune responses. Ophthalmic and systemic complications related to the implantation procedure are summarized in Table 2.

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S2213671113001306-1398

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

Animals were sacrificed and perfusion-fixed at 4 weeks posttransplantation and the recovered grafts then sectioned. Microscopic inspection revealed that the grafts had been maintained as a largely intact and continuous cell monolayer. A positive pan-cytokeratin (pCK) signal, an established RPE marker, was confirmed for fetal and adult hRPE monolayers (Figures 3A and 3C). Additional pCK reactivity was seen “underneath” the cell carrier, likely from host RPE. Moreover, fetal and adult hRPE on PET carriers stained positively for the human-specific marker SC121, confirming survival of human RPE for 1 month as a monolayer (Figure 3). Costaining of SC121 with an antibody to MCT1 and Ezrin, both apical membrane markers, further confirmed that the RPE were (still) polarized (Figure 3). SC121+ RPE transplants were negative for the expression of the cell-cycle marker ki67, the proliferation marker phosphohistone H3, and for the apoptotic marker caspase-3 (Figures 3F–3H), indicating absence of proliferation and apoptosis. We estimated the total human RPE cell survival to be approximately 95% after 1 month by using the SC121 positivity; we measured the total length of the carrier and the length of SC121 stain and calculated the percent coverage of SC121 over the total carrier length. On transmission electron micrography (TEM), polarized RPE cells were observed on the PET carriers from both fetal and adult transplants (Figures 3I and 3J). These results confirm survival of polarized human RPE from fetal and adult donors xenografted into rabbit SRS over 4 weeks.

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S2213671113001306-1404

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

The Spectralis HRA/OCT device (Heidelberg Engineering) was used to acquire laser-interferometric reflectance OCT images of the retina and choroid, with resolutions comparable to a light microscopic section. Longitudinal and transversal line OCT scans through the center of the implant were taken with the 30-degrees-of-visual-field setting of the Spectralis. Volume scans were obtained with 60 μm distance between each scan with the device set to 20 × 20 degrees of visual field centered on the implant. To approximate human optical parameters to rabbit eyes, the Spectralis’ corneal curvature settings were set by default to 4.2 mm. Red-free and infrared cSLO images were taken at 30 degrees of visual field in the HRA mode of the device.

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S2213671113001306-1520

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

(F) Adult hRPE cultures display expression of markers typical of native RPE in their polarized localization. DAPI is cyan, whereas all other immunofluorescence is gold. Claudin 19, ezrin, ZO1, and MCT1 are preferentially located on the apical side. RPE65 and CRALBP are cytoplasmic. The scale bars represent 10 μm.

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S2213671113001306-885

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

(A) Fetal hRPE stained for pan-cytokeratin (scale bar, 50 μm); inset shows section overview stained with hematoxylin/eosin (scale bar, 200 μm).

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S2213671113001306-907

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

(B) Fetal hRPE stained for SC121 (red) and MCT1 (green; scale bars, 125 μm and 25 μm [inset]).

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S2213671113001306-908

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

(D–H) Adult hRPE stained for SC121 (red). (D) Adult hRPE stained for MCT1 (green). (E) Human adult RPE stained for ezrin (green). hRPEs transplanted into rabbit SRS show absence of expression of ki67 (F), phosphohistone H3 (G), and caspase-3 (H). Polarized fetal and adult hRPE cells were found in TEM (I and J). Nuclei with regular chromatin were found in the basal compartment, a basal lamina ([I], large black arrowhead) had formed between the xenograft and PET carrier (black asterisks). Melanosomes (M) in multiple stages, some microvilli abutting to the atrophic neural retina (NR), and junctional structures with desmosomes (small black arrowhead) and tight junctions (red arrowhead) were discerned apically. Mitochondria (MC) were seen in the basolateral part of the cell. Detachment from cell carrier (asterisk) in (J) is a histologic processing artifact. Left images in (I) and (J) taken at 10,500× magnification; right micrographs are rectangular zone in left at 25,000×; scale bars represent 2 μm/inset 0.2 μm distance in (I) and (J).

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S2213671113001306-910

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

30. The method of claim 27, wherein the precursor material has an average particle size (D50) in the range from 7-13 microns.

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CA2806915A1_30

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

31. The method of claim 16, wherein the precursor material has a tap density in the range from 0.8-2.8 g/cm3.

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CA2806915A1_31

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

33. The method of claim 16, wherein the precursor material has a surface area in the range from 2-20 m2/g.

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CA2806915A1_33

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

9. The precursor material of claim 1, wherein the precursor material has a tap density in the range from 0.8-2.8 g/cm3.

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CA2806915A1_9

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

10. Precursor material according to claim 9, wherein the precursor material has a tap density of 1.8-2.3 g/cm3range.

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CN103108833A_10

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

11. Precursor material according to claim 1, wherein the precursor material in the surface area of 2-20m2/g range.

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CN103108833A_11

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

12. Precursor material according to claim 11, wherein the precursor material in the surface area of 2-8m2/g range.

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CN103108833A_12

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

13. Precursor material according to claim 1, wherein the precursor materials within the sodium level is less than 500 ppm.

train

CN103108833A_13

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

14. Precursor material according to claim 13, wherein the precursor materials within the sodium level is less than 300 ppm.

train

CN103108833A_14

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

21. Method according to claim 16, wherein the alkaline hydroxide pH of the solution was maintained at about 11-13 within a range.

train

CN103108833A_21

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

28. Method according to claim 16, wherein in the reactor at about 50-70 °C performed at a temperature in the range of co-precipitation.

train

CN103108833A_28

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

29. Method according to claim 16, wherein the precursor material has an average particle diameter (D50) in the 3-30 microns.

train

CN103108833A_29

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

30. Method according to claim 27, wherein the precursor material has an average particle diameter (D50) at 7-13 microns.

train

CN103108833A_30

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

31. Method according to claim 16, wherein the precursor material has a tap density is 0.8-2.8 g/cm3range.

train

CN103108833A_31

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

32. Method according to claim 31, wherein the precursor material has a tap density of 1.8-2.3 g/cm3range.

train

CN103108833A_32

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

33. Method according to claim 16, wherein the precursor material in the surface area of 2-20m2/g range.

train

CN103108833A_33

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

34. Method according to claim 33, wherein the precursor material in the surface area of 2-8m2/g range.

train

CN103108833A_34

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

35. Method according to claim 16, wherein the precursor materials within the sodium level is less than 500 ppm.

train

CN103108833A_35

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

36. Method according to claim 35, wherein the precursor materials within the sodium level is less than 300 ppm.

train

CN103108833A_36

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

7. Precursor material according to claim 1, wherein the precursor material has an average particle diameter (D50) in the 3-30 microns.

train

CN103108833A_7

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

8. Precursor material according to claim 7, wherein the precursor material has an average particle diameter (D50) at 7-13 microns.

train

CN103108833A_8

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

9. Precursor material according to claim 1, wherein the precursor material has a tap density is 0.8-2.8 g/cm3range.

train

CN103108833A_9

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

4. 1 according to claim-any one of claims 3 Li-Ni composite oxide particles, characterized in: An average particle diameter 1-20 µm, a specific surface area BET 0.1-1.6 m2/g.

train

CN104704659A_4

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

5. Claim 1-4 of, any one Li-Ni producing composite oxide particles, characterized in: With powder of a lithium compound Ni-Co hydroxide particles mixed powder, firing the obtained mixture, Ni-Co hydroxide particles obtained by powder, aqueous solution comprising a metal sulfate, aqueous ammonia and aqueous sodium hydroxide mixed, controls such that the reaction tank ammonia concentration is 1.4 mol/L or less, and the (ammonia concentration of the reaction vessel)/(concentration of remaining reaction tank hvdroxide) of 6 or more, obtaining Ni-Co hydroxide.

train

CN104704659A_5

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

6. Claim 1-4 of, any one Li-Ni producing composite oxide particles, characterized in: A powder of a lithium compound, Ni-Co hydroxide particle powder, aluminum powder and/or zirconium compound mixing powder of the compound, firing the obtained mixture, Ni-Co hydroxide particles obtained by powder, aqueous solution comprising a metal sulfate, aqueous ammonia and aqueous sodium hydroxide mixture, ammonia concentration is 1.4 mol/L so that the reaction tank is controlled below, and the (ammonia concentration of the reaction vessel)/(concentration of the reaction vessel remaining hvdroxide) of 6 or more, obtaining Ni-Co hydroxide.

train

CN104704659A_6

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

8. The lithium transition metal oxide according to claim 1, wherein the average particle diameter D50 of the transition metal precursor is 1 μm to 30 μm.

train

CN104884390B_8

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

1. A method for producing transition metal composite hydroxide particles serving as a precursor of a positive electrode active material for a nonaqueous electrolyte secondary battery by a crystallization reaction, comprising: a nucleus-forming step of controlling an aqueous solution for nucleus formation containing at least a transition metal-containing metal compound and an ammonium ion donor so that the aqueous solution for nucleus formation has a pH of 12.0 to 14.0 at a liquid temperature of 25 ℃ and forming nuclei, a particle growth step of controlling the aqueous solution for particle growth containing the nuclei obtained in the nucleus production step so that the pH of the aqueous solution for particle growth becomes lower than that in the nucleus production step at a liquid temperature of 25 ℃ and 10.5 to 12.0 to grow the nuclei, the reaction environment in the initial stage of the nucleus generation step and the particle growth step is a non-oxidizing environment having an oxygen concentration of 5 vol% or less, and the reaction environment is switched from the non-oxidizing environment to an oxidizing environment having an oxygen concentration of more than 5 vol% and then from the oxidizing environment to a non-oxidizing environment having an oxygen concentration of 5 vol% or less by performing environmental control at least once in the particle growth step.

train

CN105122517A_1

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

16. A positive electrode active material for nonaqueous electrolyte secondary battery, wherein, the positive electrode active material for a nonaqueous electrolyte secondary battery is composed of secondary particles formed by aggregating a plurality of primary particles, the secondary particles have a central portion of a hollow structure or a hollow structure, and have at least a space portion where the primary particles are not present and a shell portion electrically connected to the central portion outside the central portion, the secondary particles have an average particle diameter of 1 to 15 [ mu ] m, and the [ (d90-d 10)/average particle diameter ] as an index indicating the width of the particle size distribution is 0.7 or less.

train

CN105122517A_16

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

19. The positive electrode active material for a nonaqueous electrolyte secondary battery according to any one of claims 16 to 18, wherein the specific surface area is 0.7m2/g～3.0m2/g。

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CN105122517A_19

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

7. Transition metal composite hydroxide particles which are precursors of positive electrode active materials for nonaqueous electrolyte secondary batteries, wherein, the transition metal composite hydroxide particles are composed of a plurality of plate-like primary particles and secondary particles formed by aggregating fine primary particles smaller than the plate-like primary particles, the secondary particles have a central portion formed by the aggregation of the plate-like primary particles, and have at least one layered structure formed by the lamination of a low-density portion formed by the aggregation of the fine primary particles and a high-density portion formed by the aggregation of the plate-like primary particles outside the central portion, the secondary particles have an average particle diameter of 1 to 15 [ mu ] m, and the [ (d90-d 10)/average particle diameter ] as an index indicating the width of the particle size distribution is 0.65 or less.

train

CN105122517A_7

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

1. Transition metal composite hydroxide particles which are precursors of positive electrode active materials for nonaqueous electrolyte secondary batteries, wherein, the transition metal composite hydroxide particles are composed of a plurality of plate-like primary particles and secondary particles formed by aggregating fine primary particles smaller than the plate-like primary particles, the secondary particles have a central portion formed by the aggregation of the plate-like primary particles, and have a layered structure formed by the lamination of a low-density portion formed by the aggregation of the fine primary particles and a high-density portion formed by the aggregation of the plate-like primary particles outside the central portion, the average value of the ratio of the outer diameter of the central portion to the particle diameter of the secondary particles is 30 to 80%, and the average value of the ratio of the thickness of the high-density portion in the diameter direction to the particle diameter of the secondary particles is 5 to 25%, the secondary particles have an average particle diameter of 1 to 15 [ mu ] m, and the [ (d90-d 10)/average particle diameter ] as an index indicating the width of the particle size distribution is 0.65 or less.

train

CN105122517B_1

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

11. A method for producing transition metal composite hydroxide particles serving as a precursor of a positive electrode active material for a nonaqueous electrolyte secondary battery by a crystallization reaction, the method comprising: a nucleus-forming step of controlling an aqueous solution for nucleus formation containing at least a transition metal-containing metal compound and an ammonium ion donor so that the aqueous solution for nucleus formation has a pH of 12.0 to 14.0 at a liquid temperature of 25 ℃ and forming nuclei, a particle growth step of controlling the aqueous solution for particle growth containing the nuclei obtained in the nucleus production step so that the pH of the aqueous solution for particle growth becomes lower than that in the nucleus production step at a liquid temperature of 25 ℃ and 10.5 to 12.0 to grow the nuclei, and the reaction environment at the initial stage of the nucleus generation step and the particle growth step is a non-oxidizing environment having an oxygen concentration of 5% by volume or less, in the particle growth step, the environmental control is performed 2 or more times, and the environmental control is performed by: the reaction environment is switched from the non-oxidizing environment to an oxidizing environment in which the oxygen concentration exceeds 5% by volume, and then from the oxidizing environment to a non-oxidizing environment in which the oxygen concentration is 5% by volume or less, the total crystal reaction time in the oxidizing environment in the particle growth step is 3% to 30% of the total time in the particle growth step, and the crystal reaction time in each oxidizing environment is 1% or more of the total time in the particle growth step.

train

CN105122517B_11

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

19. A positive electrode active material for a nonaqueous electrolyte secondary battery, which is represented by the general formula (B): li1+uNixMnyCozMtO2The positive electrode active material is composed of lithium transition metal composite oxide particles having a hexagonal crystal structure and a layered structure, and in the general formula (B), -0.05. ltoreq. u.ltoreq.0.50, x + y + z + t 1, 0.3. ltoreq. x.ltoreq.0.95, 0.05. ltoreq. y.ltoreq.0.55, 0. ltoreq. z.ltoreq.0.4, 0. ltoreq. t.ltoreq.0.1, M is one or more additive elements selected from Mg, Ca, Al, Ti, V, Cr, Zr, Nb, Mo, Hf, Ta, W, the positive electrode active material for a nonaqueous electrolyte secondary battery is composed of secondary particles formed by aggregating a plurality of primary particles, the secondary particles have a central portion having a hollow structure or a hollow structure, and a space portion having no primary particles and an outer shell portion electrically connected to the central portion outside the central portion, and the average value of the ratio of the outer diameter of the central portion to the particle diameter of the secondary particles is 30 to 80%, the average value of the ratio of the thickness of the outer shell portion in the diameter direction to the particle diameter of the secondary particles is 5 to 25%, the secondary particles have an average particle diameter of 1 to 15 [ mu ] m, and the [ (d90-d 10)/average particle diameter ] as an index indicating the width of the particle size distribution is 0.7 or less.

train

CN105122517B_19

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

20. The positive electrode active material for a nonaqueous electrolyte secondary battery according to claim 19, wherein the specific surface area is 0.7m2/g～3.0m2/g。

train

CN105122517B_20

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

21. A positive electrode active material for a nonaqueous electrolyte secondary battery, which is represented by the general formula (B): li1+uNixMnyCozMtO2The positive electrode active material is composed of lithium transition metal composite oxide particles having a hexagonal crystal structure and a layered structure, and in the general formula (B), -0.05. ltoreq. u.ltoreq.0.50, x + y + z + t 1, 0.3. ltoreq. x.ltoreq.0.95, 0.05. ltoreq. y.ltoreq.0.55, 0. ltoreq. z.ltoreq.0.4, 0. ltoreq. t.ltoreq.0.1, M is one or more additive elements selected from Mg, Ca, Al, Ti, V, Cr, Zr, Nb, Mo, Hf, Ta, W, the positive electrode active material for a nonaqueous electrolyte secondary battery is composed of secondary particles formed by aggregating a plurality of primary particles, the secondary particles have a central portion of a hollow structure or a hollow structure, and have at least a space portion where the primary particles are not present and an outer shell portion electrically connected to the central portion outside the central portion, and at least one inner shell portion between the space portion and the outer shell portion, the secondary particles have an average particle diameter of 1 to 15 [ mu ] m, and the [ (d90-d 10)/average particle diameter ] as an index indicating the width of the particle size distribution is 0.7 or less.

train

CN105122517B_21

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

23. The positive electrode active material for a nonaqueous electrolyte secondary battery according to claim 21 or 22, wherein the specific surface area is 0.7m2/g～3.0m2/g。

train

CN105122517B_23

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

4. Transition metal composite hydroxide particles which are precursors of positive electrode active materials for nonaqueous electrolyte secondary batteries, wherein, the transition metal composite hydroxide particles are composed of a plurality of plate-like primary particles and secondary particles formed by aggregating fine primary particles smaller than the plate-like primary particles, the secondary particles have a central portion formed by the aggregation of the plate-like primary particles, and have a laminated structure in which a low-density portion formed by the aggregation of the fine primary particles and a high-density portion formed by the aggregation of the plate-like primary particles are laminated outside the central portion, the secondary particles have an average particle diameter of 1 to 15 [ mu ] m, and the [ (d90-d 10)/average particle diameter ] as an index indicating the width of the particle size distribution is 0.65 or less.

train

CN105122517B_4

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

8. A method for producing transition metal composite hydroxide particles serving as a precursor of a positive electrode active material for a nonaqueous electrolyte secondary battery by a crystallization reaction, the method comprising: a nucleus-forming step of controlling an aqueous solution for nucleus formation containing at least a transition metal-containing metal compound and an ammonium ion donor so that the aqueous solution for nucleus formation has a pH of 12.0 to 14.0 at a liquid temperature of 25 ℃ and forming nuclei, a particle growth step of controlling the aqueous solution for particle growth containing the nuclei obtained in the nucleus production step so that the pH of the aqueous solution for particle growth becomes lower than that in the nucleus production step at a liquid temperature of 25 ℃ and 10.5 to 12.0 to grow the nuclei, and the reaction environment at the initial stage of the nucleus generation step and the particle growth step is a non-oxidizing environment having an oxygen concentration of 5% by volume or less, in the particle growth step, an environmental control is performed once in a range of 5% to 35% of the total time of the particle growth step from the start of the particle growth step, the environmental control being: the reaction environment is switched from the non-oxidizing environment to an oxidizing environment having an oxygen concentration of more than 5% by volume, and then from the oxidizing environment to a non-oxidizing environment having an oxygen concentration of 5% by volume or less, so that the crystallization reaction time in the oxidizing environment is 3% to 20% of the total time of the particle growth step.

train

CN105122517B_8

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

1. A method for producing a transition metal composite oxide, comprising: a 1 st step of preparing a 1 st internal formation metal salt aqueous solution and a 2 nd internal formation metal salt aqueous solution containing nickel, cobalt, and manganese and having different concentrations of the nickel, cobalt, and manganese from each other; step 2, supplying a chelating agent and an aqueous alkali solution into the reactor; a 3 rd step of continuously supplying and mixing the 1 st interior-forming metal salt aqueous solution, the chelating agent, and the alkali aqueous solution to the reactor, and culturing particles having a fixed concentration of nickel, cobalt, and manganese and containing the 1 st interior having a radius of r1(0.2 um. ltoreq. r 1. ltoreq.5 um); and a 4 th step of mixing and supplying the 1 st inside forming metal salt aqueous solution and the 2 nd inside forming metal salt aqueous solution in a mixing ratio gradually changing from 100 v%: 0 v% to 0 v%: 100 v%, while mixing the chelating agent and the alkali aqueous solution into the reactor, to form particles containing the 2 nd inside having a radius r2(r2 ≦ 10um) in the 1 st inside profile, characterized in that, the concentration of the alkaline aqueous solution added in the reactor in the 2 nd step is adjusted according to the nickel concentration of the particles produced in the 3 rd step before the 3 rd step, and the concentration of the alkaline aqueous solution of the reaction solution in the 2 nd step is adjusted to 0.25g/L to 0.5g/L so that the pH of the solution in the reactor in the 2 nd step is adjusted to 11.8 to 12.3.

train

CN105594029B_1

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

3. The method for producing a transferred-metal composite oxide according to claim 1, in the steps 1 to 4, the aqueous solution of the metal salt for forming the inside of the 1 st compartment, the chelating agent, and the aqueous alkali solution are mixed in the reactor so that the particle size distribution of the particles formed after the reaction for 30 minutes has D50 of 4um or less.

train

CN105594029B_3

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

4. The method for producing a transferred-metal composite oxide according to claim 1, further comprising the step 5: drying or heat-treating the transition metal composite oxide obtained by performing the 1 st to 4 th steps, and the average diameter of the transfer metal composite oxide particles produced in the 5 th step is 5 to 10 um.

train

CN105594029B_4

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

9. A method for producing a transition metal composite oxide, the method comprises the following steps: manufacturing a 1 st metal salt aqueous solution containing nickel, manganese and cobalt; manufacturing an aqueous solution of a 2 nd metal salt containing nickel, manganese and cobalt; mixing an alkaline aqueous solution and an aqueous ammonia solution in a reactor, adjusting the concentration of the alkaline aqueous solution according to the nickel concentration of the 1 st metal salt aqueous solution, and adjusting the pH value in the reaction solution to 11.8 to 12.3; and supplying a 1 st mixed metal salt aqueous solution mixing the 1 st metal salt aqueous solution and the 2 nd metal salt aqueous solution, ammonia, and an alkaline aqueous solution into the reactor, and a mixing ratio of the 1 st metal salt aqueous solution and the 2 nd metal salt aqueous solution in the 1 st mixed metal salt aqueous solution is 0 v% or more and 100 v% or less.

train

CN105594029B_9

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

3. A positive electrode active material for a lithium secondary battery according to claim 1 or 2, characterized in, lithium transition metal composite oxide has a particle size distribution of not more than a maximum value of 2.

train

CN105594031A_3

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

5. The positive electrode for lithium secondary battery active material according to claim 4 in which a manufacturing method, characterized in, of a tap density of 1.4 g/cc or more precursors.

train

CN105594031A_5

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

12. For a rechargeable battery positive electrode material of lithium metal oxide powder, having the general formula Li_1+a M_1-a O₂, wherein M=Ni_x Mn_y Co_z A_v, Aas a dopant, wherein<0.30,0.55 ≤ y ≤ 0.80 0.10 ≤ a ≤ 0.25,0.10 ≤ x, and the 0<z ≤ 0.30, v ≤ 0.05, and the x + y + z + v=1, powder having a particle size distribution 10μm ≤ D50 ≤ 20 µm, 0.9 ≤ BET ≤ 5 has a specific surface, with BET m² /grepresented, also include sodium and sulfur impurities powder, wherein sodium % by weight represented by (Na_weight) and sulfur (S_weight) sum of the contents (2 * Na_weight) +S_weight exceeds 0.4 wt % and less than 1.6% by weight, and wherein the molar ratio of sodium to sulfur (Na/S) was 0.4<Na/S<2.

train

CN106795008A_12

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

17. Method according to claim 13, wherein the reactor in the NH3concentration is less than 5.0 g/L.

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CN106795008A_17

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

20. Method according to claim 13, wherein the seeds have a D50 mean particle size of 3 μm between 0.1 and an.

train

CN106795008A_20

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

7. For a rechargeable battery positive electrode material of a lithium metal oxide powder, having the general formula Li_1+a M_1-a O₂, wherein M=Ni_x Mn_y Co_z A_v, Aas a dopant, wherein-0.05 ≤ a ≤ 0.25,0.20 ≤ x ≤ 0.90,0.10 ≤ y ≤ 0.67, and a 0.10 ≤ z ≤ 0.40, v ≤ 0.05, and the x + y + z + v=1, powder having a particle size distribution 10μm ≤ D50 ≤ 20 µm, 0.9 ≤ BET ≤ 5 has a specific surface, with BET m² /grepresented, sodium and sulfur powder also includes impurities, wherein % by weight sodium expressed by (Na_weight) and sulfur (S_weight) sum of the contents (2 * Na_weight) +S_weight more than 0.4% and less than 1.6% by weight of the weight, and wherein the molar ratio of sodium to sulfur (Na/S) was 0.4<Na/S<2.

train

CN106795008A_7

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

1. A carbonate precursor compound for the manufacture of lithium metal (M) oxide powder useful as an active positive electrode material in lithium ion batteries, M comprising 20 to 90 mol% Ni, 10 to 70 mol% Mn and 10 to 40 mol% Co, the precursor further comprising sodium and sulfur impurities, wherein the molar ratio of sodium to sulfur (Na/S) is 0.4<Na/S<2, and wherein the sodium (Na) is expressed in weight%<Sub>Weight (D)</Sub>) And sulfur (S)<Sub>Weight (D)</Sub>) Sum of contents (2. multidot. Na)<Sub>Weight (D)</Sub>)+S<Sub>Weight (D)</Sub>More than 0.4 wt% and less than 1.6 wt%.

train

CN106795008B_1

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

11. A lithium metal oxide powder for use in a positive electrode material in a rechargeable battery having the general formula Li<Sub>1+a</Sub>M<Sub>1-a</Sub>O<Sub>2</Sub>Wherein M is Ni<Sub>x</Sub>Mn<Sub>y</Sub>Co<Sub>z</Sub>A<Sub>v</Sub>A is a dopant, wherein a is more than or equal to 0.10 and less than or equal to 0.25, and x is more than or equal to 0.10 and less than or equal to 0.25<Y is more than or equal to 0.30, 0.55 and less than or equal to 0.80, and 0<z 0.30, v 0.05 and x + y + z + v 1, said powder having a particle size distribution of 10 [ mu ] m 20 [ mu ] m D50, 0.9 BET 5 in m<Sup>2</Sup>Expressed in terms of/g, said powder further comprising sodium and sulphur impurities, wherein said sodium (Na) is expressed in weight%<Sub>Weight (D)</Sub>) And sulfur (S)<Sub>Weight (D)</Sub>) Sum of contents (2. multidot. Na)<Sub>Weight (D)</Sub>)+S<Sub>Weight (D)</Sub>More than 0.4% by weightAnd less than 1.6 wt%, and wherein the molar ratio of sodium to sulfur (Na/S) is 0.4<Na/S<2。

train

CN106795008B_11

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

16. The method of claim 12, wherein NH in the reactor3The concentration is lower than 5.0 g/L.

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CN106795008B_16

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

19. The method of claim 12, wherein the seed crystals have a median particle diameter D50 of between 0.1 and 3 μ ι η.

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CN106795008B_19

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

5. The carbonate precursor compound of claim 1, wherein the sodium content is between 0.1 and 0.7 wt% and the sulfur content is between 0.2 and 0.9 wt%.

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CN106795008B_5

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

6. A lithium metal oxide powder for use in a positive electrode material in a rechargeable battery having the general formula Li1+aM1-aO2Wherein M is NixMnyCozAvA is a dopant, where-0.05. ltoreq. a.ltoreq.0.25, 0.20. ltoreq. x.ltoreq.0.90, 0.10. ltoreq. y.ltoreq.0.67, and 0.10. ltoreq. z.ltoreq.0.40, v.ltoreq.0.05, and x + y + z + v.ltoreq.1, the powder having a particle size distribution of 10 μm. ltoreq.D 50. ltoreq.20 μm, a specific surface area of 0.9. ltoreq. BET 5 in m<Sup>2</Sup>Expressed in terms of/g, said powder further comprising sodium and sulphur impurities, wherein said sodium (Na) is expressed in weight%<Sub>Weight (D)</Sub>) And sulfur (S)<Sub>Weight (D)</Sub>) ComprisesSum of the amounts (2 Na)<Sub>Weight (D)</Sub>)+S<Sub>Weight (D)</Sub>More than 0.4 wt% and less than 1.6 wt%, and wherein the molar ratio of sodium to sulfur (Na/S) is 0.4<Na/S<2。

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CN106795008B_6

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

5. The transition metal oxide precursor of claim 1, wherein the transition metal oxide precursor has a tap density of from 1.0g/cc to 2.5 g/cc.

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CN107074588A_5

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

4. The transition metal oxide precursor of claim 1, wherein the transition metal oxide precursor has a tap density of from 1.0g/cc to 2.5 g/cc.

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CN107074588B_4

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

6. Composite transition metal oxide-based precursor according to claim 5, characterized in, Average particle diameter of primary particles of the platy or acicular form 0.01-0.8 µm range, An average particle size D50 of the secondary particles are 3-30 μm range.

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CN107305946A_6

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

7. Composite transition metal oxide-based precursor according to claim 1, characterized in, Precursor has a tap density less than 2.0 g/cc to.

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CN107305946A_7

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

2. The anode active material precursor according to claim 1, characterized in, determined by the method according to nitrogen adsorption BET specific surface area of 50-200 m2/gof the range.

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CN107316984A_2

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

4. Composite transition metal oxide-based precursor according to claim 3, characterized in, Average particle diameter of primary particles of the platy or acicular form 0.01-0.8 µm range, exists within a lot of pores on the surface or structure, The secondary particles have an average particle size D50 to 3-30 μm of the range.

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CN107342416A_4

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

5. Composite transition metal oxide-based precursor according to claim 1, characterized in, or more of the tap density of 2.0 g/cc precursor.

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CN107342416A_5

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

6. Composite transition metal oxide-based precursor according to claim 1, characterized in, according to the BET nitrogen adsorption of the precursor of the specific surface area of 5-80 m measured2/gof the range.

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CN107342416A_6

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

1. A preparation method of porous nickel-cobalt-manganese composite hydroxide is characterized by comprising the following steps: mixing a nickel salt, a cobalt salt, a manganese salt aqueous solution, a complexing agent and a precipitating agent, adding a loosening agent, adjusting the pH of the system to 10-12, and heating, stirring and reacting to obtain a porous nickel-cobalt-manganese composite hydroxide precipitate; and washing and drying to obtain the porous nickel-cobalt-manganese composite hydroxide.

train

CN107732212A_1

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You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

8. The method for preparing a porous nickel-cobalt-manganese composite hydroxide according to claim 1, characterized in that: the dosage of the complexing agent is 1-20 g/L; the total molar ratio of the used precipitator to the nickel element, the cobalt element and the manganese element is 2: 1; the heating and stirring reaction is carried out at a rotating speed of 100-300 rpm and a temperature of 40-80 ℃ for 20-200 h.

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CN107732212A_8

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

14. The method of claim 9, wherein the pH of the first mixture in the first process is adjusted to be in the range of 10 to 11 and the pH of the second mixture in the second process is adjusted to be in the range of 11.5 to 12.0.

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CN108281650A_14

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

10. The positive electrode active material for a secondary battery according to claim 8, having a tap density of 1.7 to 3.0 g/cc.

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CN108701827A_10

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.

5. The precursor of the positive electrode active material for a secondary battery according to claim 1, having an average particle diameter (D) of 7 to 20 μm50) And 5.0m2G to 30.0m2BET specific surface area in g.

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CN108701827A_5

bm

You are an expert at extracting quantity, units and their related context from text. Given a paragraph below identify each quantity and its related unit and related context, i.e. the measured entity and measured property if they exist.