jackkuo/LLM-Enzyme-Kinetics-Golden-Benchmark

ID_gold int64 1 4.37k	pubmed_id int64 1.88M 33.4M	Enzyme stringclasses 283 values	Organism stringclasses 232 values	Substrate stringlengths 1 81 ⌀	km(mM) stringlengths 1 8 ⌀	kcat(s^-1) stringlengths 1 10 ⌀	km_kcat(mM^-1s^-1) stringlengths 1 12 ⌀	Commentary[Temp] stringclasses 70 values	Commentary[pH] stringclasses 102 values	Commentary[Mutant] stringclasses 806 values	Commentary[Cosubstrate] stringclasses 200 values	Reference[Table/Text] stringclasses 4 values
349	26,002,966	AKR1C34	Golden hamster	5α-Pregnan-20α-ol-3-one	0.0014	0.013	9.333333	25°C	7.4	WT	1.0 mM NAD+	Table
350	26,002,966	AKR1C35	Golden hamster	5α,3β-DIOL	0.0045	0.056667	12.666667	25°C	7.4	WT	1.0 mM NAD+	Table
351	26,002,966	AKR1C35	Golden hamster	5-Androstene-3β,17β-diol	0.0033	0.031667	9.666667	25°C	7.4	WT	1.0 mM NAD+	Table
352	26,002,966	AKR1C35	Golden hamster	5α,3α-DIOL	0.0072	0.043333	6	25°C	7.4	WT	1.0 mM NAD+	Table
353	26,002,966	AKR1C35	Golden hamster	4-Androstene-3β,17β-diol	0.0081	0.043333	5.5	25°C	7.4	WT	1.0 mM NAD+	Table
354	26,002,966	AKR1C35	Golden hamster	5β-Androstane-3α,17β-diol	0.0039	0.012	3	25°C	7.4	WT	1.0 mM NAD+	Table
355	26,002,966	AKR1C35	Golden hamster	5β-Androstane-3β,17β-diol	0.015	0.036667	2.5	25°C	7.4	WT	1.0 mM NAD+	Table
356	26,002,966	AKR1C34	Golden hamster	5α,3β-DIOL	null	null	null	25°C	7.4	WT	1.0 mM NAD+	Table
357	26,002,966	AKR1C34	Golden hamster	5-Androstene-3β,17β-diol	null	null	null	25°C	7.4	WT	1.0 mM NAD+	Table
358	26,002,966	AKR1C34	Golden hamster	5α,3α-DIOL	null	null	null	25°C	7.4	WT	1.0 mM NAD+	Table
359	26,002,966	AKR1C34	Golden hamster	4-Androstene-3β,17β-diol	0.002	null	null	25°C	7.4	WT	1.0 mM NAD+	Table
360	26,002,966	AKR1C34	Golden hamster	5β-Androstane-3α,17β-diol	0.0007	0.012167	16.666667	25°C	7.4	WT	1.0 mM NAD+	Table
361	26,002,966	AKR1C34	Golden hamster	5β-Androstane-3β,17β-diol	null	null	null	25°C	7.4	WT	1.0 mM NAD+	Table
362	26,002,966	AKR1C35	Golden hamster	3β-Hydroxyprogesterone	0.0063	0.003	0.466667	25°C	7.4	V54A	1.0 mM NAD+	Table
363	26,002,966	AKR1C35	Golden hamster	3β-Hydroxyprogesterone	0.0007	0.02	28.333333	25°C	7.4	C310F	1.0 mM NAD+	Table
364	26,002,966	AKR1C35	Golden hamster	3β-Hydroxyprogesterone	0.0055	0.0013	0.233333	25°C	7.4	V54A/C310F	1.0 mM NAD+	Table
365	26,002,966	AKR1C35	Golden hamster	5α-Androstan-3β-ol-17-one	0.0062	0.001233	0.2	25°C	7.4	V54A	1.0 mM NAD+	Table
366	26,002,966	AKR1C35	Golden hamster	5α-Androstan-3β-ol-17-one	0.0023	0.04	16.666667	25°C	7.4	C310F	1.0 mM NAD+	Table
367	26,002,966	AKR1C35	Golden hamster	5α-Androstan-3β-ol-17-one	0.0037	0.000883	0.233333	25°C	7.4	V54A/C310F	1.0 mM NAD+	Table
368	26,002,966	AKR1C35	Golden hamster	5α,3α-DIOL	0.002	0.00115	0.583333	25°C	7.4	V54A	1.0 mM NAD+	Table
369	26,002,966	AKR1C35	Golden hamster	5α,3α-DIOL	0.0008	0.045	56.666667	25°C	7.4	C310F	1.0 mM NAD+	Table
370	26,002,966	AKR1C35	Golden hamster	5α,3α-DIOL	0.0051	0.0003	0.058333	25°C	7.4	V54A/C310F	1.0 mM NAD+	Table
371	26,002,966	AKR1C35	Golden hamster	17β-Estradiol	0.0016	0.003333	2.166667	25°C	7.4	V54A	1.0 mM NAD+	Table
372	26,002,966	AKR1C35	Golden hamster	17β-Estradiol	0.0005	0.005167	10.333333	25°C	7.4	C310F	1.0 mM NAD+	Table
373	26,002,966	AKR1C35	Golden hamster	17β-Estradiol	0.0023	0.001467	0.633333	25°C	7.4	V54A/C310F	1.0 mM NAD+	Table
374	26,002,966	AKR1C35	Golden hamster	S-Tetralol	0.068	0.023333	0.35	25°C	7.4	V54A	1.0 mM NAD+	Table
375	26,002,966	AKR1C35	Golden hamster	S-Tetralol	0.06	0.093333	1.55	25°C	7.4	C310F	1.0 mM NAD+	Table
376	26,002,966	AKR1C35	Golden hamster	S-Tetralol	0.031	0.018333	0.583333	25°C	7.4	V54A/C310F	1.0 mM NAD+	Table
377	28,405,665	PcPAL	Petroselinum crispum	L-Phenylalanine	0.083	0.694	8.361	30°C	8.8	WT	0.1 M Tris and 0.12 M NaCl	Table
378	28,405,665	PcPAL	Petroselinum crispum	L-Styrylalanine	4.384	0.0471	0.0107	30°C	8.8	WT	0.1 M Tris and 0.12 M NaCl	Table
379	28,405,665	PcPAL	Petroselinum crispum	L-Phenylalanine	0.086	0.173	2.011	30°C	8.8	F137V	0.1 M Tris and 0.12 M NaCl	Table
380	28,405,665	PcPAL	Petroselinum crispum	L-Styrylalanine	0.186	0.422	2.269	30°C	8.8	F137V	0.1 M Tris and 0.12 M NaCl	Table
381	28,405,665	PcPAL	Petroselinum crispum	L-Phenylalanine	1.732	0.283	0.163	30°C	8.8	F137A	0.1 M Tris and 0.12 M NaCl	Table
382	28,405,665	PcPAL	Petroselinum crispum	L-Styrylalanine	1.173	0.132	0.1125	30°C	8.8	F137A	0.1 M Tris and 0.12 M NaCl	Table
383	28,405,665	PcPAL	Petroselinum crispum	L-Phenylalanine	4.969	0.052	0.0104	30°C	8.8	F137G	0.1 M Tris and 0.12 M NaCl	Table
384	28,405,665	PcPAL	Petroselinum crispum	L-Styrylalanine	4.12	0.0345	0.0083	30°C	8.8	F137G	0.1 M Tris and 0.12 M NaCl	Table
385	28,405,665	PcPAL	Petroselinum crispum	rac-1a	0.395	0.0062	0.0156	30°C	8.8	WT	0.1 M Tris and 0.12 M NaCl	Table
386	28,405,665	PcPAL	Petroselinum crispum	rac-1b	0.154	0.00024	0.0015	30°C	8.8	WT	0.1 M Tris and 0.12 M NaCl	Table
387	28,405,665	PcPAL	Petroselinum crispum	rac-1c	0.028	0.00034	0.0121	30°C	8.8	WT	0.1 M Tris and 0.12 M NaCl	Table
388	28,405,665	PcPAL	Petroselinum crispum	rac-1d	0.287	0.0032	0.0111	30°C	8.8	WT	0.1 M Tris and 0.12 M NaCl	Table
389	28,405,665	PcPAL	Petroselinum crispum	rac-1a	0.201	0.276	1.373	30°C	8.8	F137V	0.1 M Tris and 0.12 M NaCl	Table
390	28,405,665	PcPAL	Petroselinum crispum	rac-1b	0.0783	0.0786	1.004	30°C	8.8	F137V	0.1 M Tris and 0.12 M NaCl	Table
391	28,405,665	PcPAL	Petroselinum crispum	rac-1c	0.0947	0.157	1.657	30°C	8.8	F137V	0.1 M Tris and 0.12 M NaCl	Table
392	28,405,665	PcPAL	Petroselinum crispum	rac-1d	0.326	0.0099	0.0302	30°C	8.8	F137V	0.1 M Tris and 0.12 M NaCl	Table
393	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.072	4.1	57	25°C	7.2	WT	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
394	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	null	25°C	7.2	WT	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
395	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.22	8.4	38	25°C	7.2	Y93H	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
396	28,955,773	ttCK	T. thermophilus HB8	Uridine	0.36	9.2	26	25°C	7.2	Y93H	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
397	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.071	0.4	6	25°C	7.2	Y93W	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
398	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	null	25°C	7.2	Y93W	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
399	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.1	5.9	5.7	25°C	7.2	Y93F	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
400	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	0.016	25°C	7.2	Y93F	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
401	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.42	10	2.4	25°C	7.2	Y93L	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
402	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	0.15	25°C	7.2	Y93L	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
403	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.574	2.9	5.1	25°C	7.2	Y93I	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
404	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	0.092	25°C	7.2	Y93I	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
405	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.965	3.9	4.1	25°C	7.2	Y93V	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
406	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	0.079	25°C	7.2	Y93V	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
407	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.555	4.6	8.3	25°C	7.2	Y93P	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
408	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	0.097	25°C	7.2	Y93P	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
409	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.383	4.5	12	25°C	7.2	Y93A	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
410	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	0.051	25°C	7.2	Y93A	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
411	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.62	2.9	4.6	25°C	7.2	Y93G	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
412	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	0.043	25°C	7.2	Y93G	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
413	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.587	3.1	5.3	25°C	7.2	Y93T	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
414	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	0.61	25°C	7.2	Y93T	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
415	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.225	7.1	32	25°C	7.2	Y93S	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
416	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	0.29	25°C	7.2	Y93S	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
417	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.95	7.2	7.7	25°C	7.2	Y93M	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
418	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	0.048	25°C	7.2	Y93M	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
419	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.215	1.9	9	25°C	7.2	Y93C	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
420	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	0.023	25°C	7.2	Y93C	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
421	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.23	2.8	12	25°C	7.2	Y93Q	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
422	28,955,773	ttCK	T. thermophilus HB8	Uridine	1.6	1.1	0.72	25°C	7.2	Y93Q	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
423	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.47	4.1	8.7	25°C	7.2	Y93N	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
424	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	0.041	25°C	7.2	Y93N	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
425	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.186	1.5	5.8	25°C	7.2	Y93E	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
426	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	0.056	25°C	7.2	Y93E	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
427	28,955,773	ttCK	T. thermophilus HB8	Cytidine	0.236	1.4	5.8	25°C	7.2	Y93D	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
428	28,955,773	ttCK	T. thermophilus HB8	Uridine	null	null	0.056	25°C	7.2	Y93D	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
429	28,955,773	hsUCK2	Homo sapiens	Cytidine	0.138	6.4	47	25°C	7.2	H117Y	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
430	28,955,773	hsUCK2	Homo sapiens	Uridine	null	null	0.96	25°C	7.2	H117Y	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
431	28,955,773	hsUCK2	Homo sapiens	Cytidine	0.042	4.3	100	25°C	7.2	WT	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
432	28,955,773	hsUCK2	Homo sapiens	Uridine	0.058	5.2	90	25°C	7.2	WT	20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase	Table
433	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	0.28	51	182	80°C	7.5	natural WT	null	Table
434	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	0.33	48	146	80°C	7.5	recombinant WT	null	Table
435	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	0.31	2.2	7.2	80°C	7.5	Y253F	null	Table
436	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	null	0	0	50 or 80 °C	7.5	Y253W	null	Table
437	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	null	0	0	50 or 80 °C	7.5	Y253A	null	Table
438	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	0.17	3.6	21	80°C	7.5	P254G	null	Table
439	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	0.28	5.7	20	80°C	7.5	I255L	null	Table
440	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	0.13	3.2	25	80°C	7.5	I255S	null	Table
441	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	0.25	6	24	80°C	7.5	I255V	null	Table
442	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	0.19	7.9	41	80°C	7.5	I255M	null	Table
443	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	0.11	4.1	38	80°C	7.5	T256S	null	Table
444	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	0.14	4.3	31	80°C	7.5	T256A	null	Table
445	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	0.17	4.6	28	80°C	7.5	T256V	null	Table
446	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	null	0	0	50 or 80 °C	7.5	P257G	null	Table
447	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	null	0	0	50 or 80 °C	7.5	P257A	null	Table
448	11,683,888	OFOR	Sulfolobus sp. strain 7	Pyruvate	null	0	0	50 or 80 °C	7.5	P257V	null	Table

349

26,002,966

AKR1C34

Golden hamster

5α-Pregnan-20α-ol-3-one

0.0014

0.013

9.333333

25°C

7.4

WT

1.0 mM NAD+

Table

350

26,002,966

AKR1C35

Golden hamster

5α,3β-DIOL

0.0045

0.056667

12.666667

25°C

7.4

WT

1.0 mM NAD+

Table

351

26,002,966

AKR1C35

Golden hamster

5-Androstene-3β,17β-diol

0.0033

0.031667

9.666667

25°C

7.4

WT

1.0 mM NAD+

Table

352

26,002,966

AKR1C35

Golden hamster

5α,3α-DIOL

0.0072

0.043333

6

25°C

7.4

WT

1.0 mM NAD+

Table

353

26,002,966

AKR1C35

Golden hamster

4-Androstene-3β,17β-diol

0.0081

0.043333

5.5

25°C

7.4

WT

1.0 mM NAD+

Table

354

26,002,966

AKR1C35

Golden hamster

5β-Androstane-3α,17β-diol

0.0039

0.012

3

25°C

7.4

WT

1.0 mM NAD+

Table

355

26,002,966

AKR1C35

Golden hamster

5β-Androstane-3β,17β-diol

0.015

0.036667

2.5

25°C

7.4

WT

1.0 mM NAD+

Table

356

26,002,966

AKR1C34

Golden hamster

5α,3β-DIOL

null

25°C

7.4

WT

1.0 mM NAD+

Table

357

26,002,966

AKR1C34

Golden hamster

5-Androstene-3β,17β-diol

null

25°C

7.4

WT

1.0 mM NAD+

Table

358

26,002,966

AKR1C34

Golden hamster

5α,3α-DIOL

null

25°C

7.4

WT

1.0 mM NAD+

Table

359

26,002,966

AKR1C34

Golden hamster

4-Androstene-3β,17β-diol

0.002

null

25°C

7.4

WT

1.0 mM NAD+

Table

360

26,002,966

AKR1C34

Golden hamster

5β-Androstane-3α,17β-diol

0.0007

0.012167

16.666667

25°C

7.4

WT

1.0 mM NAD+

Table

361

26,002,966

AKR1C34

Golden hamster

5β-Androstane-3β,17β-diol

null

25°C

7.4

WT

1.0 mM NAD+

Table

362

26,002,966

AKR1C35

Golden hamster

3β-Hydroxyprogesterone

0.0063

0.003

0.466667

25°C

7.4

V54A

1.0 mM NAD+

Table

363

26,002,966

AKR1C35

Golden hamster

3β-Hydroxyprogesterone

0.0007

0.02

28.333333

25°C

7.4

C310F

1.0 mM NAD+

Table

364

26,002,966

AKR1C35

Golden hamster

3β-Hydroxyprogesterone

0.0055

0.0013

0.233333

25°C

7.4

V54A/C310F

1.0 mM NAD+

Table

365

26,002,966

AKR1C35

Golden hamster

5α-Androstan-3β-ol-17-one

0.0062

0.001233

0.2

25°C

7.4

V54A

1.0 mM NAD+

Table

366

26,002,966

AKR1C35

Golden hamster

5α-Androstan-3β-ol-17-one

0.0023

0.04

16.666667

25°C

7.4

C310F

1.0 mM NAD+

Table

367

26,002,966

AKR1C35

Golden hamster

5α-Androstan-3β-ol-17-one

0.0037

0.000883

0.233333

25°C

7.4

V54A/C310F

1.0 mM NAD+

Table

368

26,002,966

AKR1C35

Golden hamster

5α,3α-DIOL

0.002

0.00115

0.583333

25°C

7.4

V54A

1.0 mM NAD+

Table

369

26,002,966

AKR1C35

Golden hamster

5α,3α-DIOL

0.0008

0.045

56.666667

25°C

7.4

C310F

1.0 mM NAD+

Table

370

26,002,966

AKR1C35

Golden hamster

5α,3α-DIOL

0.0051

0.0003

0.058333

25°C

7.4

V54A/C310F

1.0 mM NAD+

Table

371

26,002,966

AKR1C35

Golden hamster

17β-Estradiol

0.0016

0.003333

2.166667

25°C

7.4

V54A

1.0 mM NAD+

Table

372

26,002,966

AKR1C35

Golden hamster

17β-Estradiol

0.0005

0.005167

10.333333

25°C

7.4

C310F

1.0 mM NAD+

Table

373

26,002,966

AKR1C35

Golden hamster

17β-Estradiol

0.0023

0.001467

0.633333

25°C

7.4

V54A/C310F

1.0 mM NAD+

Table

374

26,002,966

AKR1C35

Golden hamster

S-Tetralol

0.068

0.023333

0.35

25°C

7.4

V54A

1.0 mM NAD+

Table

375

26,002,966

AKR1C35

Golden hamster

S-Tetralol

0.06

0.093333

1.55

25°C

7.4

C310F

1.0 mM NAD+

Table

376

26,002,966

AKR1C35

Golden hamster

S-Tetralol

0.031

0.018333

0.583333

25°C

7.4

V54A/C310F

1.0 mM NAD+

Table

377

28,405,665

PcPAL

Petroselinum crispum

L-Phenylalanine

0.083

0.694

8.361

30°C

8.8

WT

0.1 M Tris and 0.12 M NaCl

Table

378

28,405,665

PcPAL

Petroselinum crispum

L-Styrylalanine

4.384

0.0471

0.0107

30°C

8.8

WT

0.1 M Tris and 0.12 M NaCl

Table

379

28,405,665

PcPAL

Petroselinum crispum

L-Phenylalanine

0.086

0.173

2.011

30°C

8.8

F137V

0.1 M Tris and 0.12 M NaCl

Table

380

28,405,665

PcPAL

Petroselinum crispum

L-Styrylalanine

0.186

0.422

2.269

30°C

8.8

F137V

0.1 M Tris and 0.12 M NaCl

Table

381

28,405,665

PcPAL

Petroselinum crispum

L-Phenylalanine

1.732

0.283

0.163

30°C

8.8

F137A

0.1 M Tris and 0.12 M NaCl

Table

382

28,405,665

PcPAL

Petroselinum crispum

L-Styrylalanine

1.173

0.132

0.1125

30°C

8.8

F137A

0.1 M Tris and 0.12 M NaCl

Table

383

28,405,665

PcPAL

Petroselinum crispum

L-Phenylalanine

4.969

0.052

0.0104

30°C

8.8

F137G

0.1 M Tris and 0.12 M NaCl

Table

384

28,405,665

PcPAL

Petroselinum crispum

L-Styrylalanine

4.12

0.0345

0.0083

30°C

8.8

F137G

0.1 M Tris and 0.12 M NaCl

Table

385

28,405,665

PcPAL

Petroselinum crispum

rac-1a

0.395

0.0062

0.0156

30°C

8.8

WT

0.1 M Tris and 0.12 M NaCl

Table

386

28,405,665

PcPAL

Petroselinum crispum

rac-1b

0.154

0.00024

0.0015

30°C

8.8

WT

0.1 M Tris and 0.12 M NaCl

Table

387

28,405,665

PcPAL

Petroselinum crispum

rac-1c

0.028

0.00034

0.0121

30°C

8.8

WT

0.1 M Tris and 0.12 M NaCl

Table

388

28,405,665

PcPAL

Petroselinum crispum

rac-1d

0.287

0.0032

0.0111

30°C

8.8

WT

0.1 M Tris and 0.12 M NaCl

Table

389

28,405,665

PcPAL

Petroselinum crispum

rac-1a

0.201

0.276

1.373

30°C

8.8

F137V

0.1 M Tris and 0.12 M NaCl

Table

390

28,405,665

PcPAL

Petroselinum crispum

rac-1b

0.0783

0.0786

1.004

30°C

8.8

F137V

0.1 M Tris and 0.12 M NaCl

Table

391

28,405,665

PcPAL

Petroselinum crispum

rac-1c

0.0947

0.157

1.657

30°C

8.8

F137V

0.1 M Tris and 0.12 M NaCl

Table

392

28,405,665

PcPAL

Petroselinum crispum

rac-1d

0.326

0.0099

0.0302

30°C

8.8

F137V

0.1 M Tris and 0.12 M NaCl

Table

393

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.072

4.1

57

25°C

7.2

WT

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

394

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

25°C

7.2

WT

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

395

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.22

8.4

38

25°C

7.2

Y93H

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

396

28,955,773

ttCK

T. thermophilus HB8

Uridine

0.36

9.2

26

25°C

7.2

Y93H

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

397

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.071

0.4

6

25°C

7.2

Y93W

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

398

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

25°C

7.2

Y93W

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

399

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.1

5.9

5.7

25°C

7.2

Y93F

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

400

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

0.016

25°C

7.2

Y93F

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

401

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.42

10

2.4

25°C

7.2

Y93L

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

402

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

0.15

25°C

7.2

Y93L

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

403

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.574

2.9

5.1

25°C

7.2

Y93I

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

404

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

0.092

25°C

7.2

Y93I

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

405

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.965

3.9

4.1

25°C

7.2

Y93V

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

406

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

0.079

25°C

7.2

Y93V

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

407

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.555

4.6

8.3

25°C

7.2

Y93P

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

408

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

0.097

25°C

7.2

Y93P

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

409

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.383

4.5

12

25°C

7.2

Y93A

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

410

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

0.051

25°C

7.2

Y93A

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

411

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.62

2.9

4.6

25°C

7.2

Y93G

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

412

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

0.043

25°C

7.2

Y93G

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

413

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.587

3.1

5.3

25°C

7.2

Y93T

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

414

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

0.61

25°C

7.2

Y93T

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

415

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.225

7.1

32

25°C

7.2

Y93S

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

416

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

0.29

25°C

7.2

Y93S

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

417

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.95

7.2

7.7

25°C

7.2

Y93M

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

418

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

0.048

25°C

7.2

Y93M

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

419

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.215

1.9

9

25°C

7.2

Y93C

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

420

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

0.023

25°C

7.2

Y93C

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

421

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.23

2.8

12

25°C

7.2

Y93Q

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

422

28,955,773

ttCK

T. thermophilus HB8

Uridine

1.6

1.1

0.72

25°C

7.2

Y93Q

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

423

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.47

4.1

8.7

25°C

7.2

Y93N

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

424

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

0.041

25°C

7.2

Y93N

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

425

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.186

1.5

5.8

25°C

7.2

Y93E

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

426

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

0.056

25°C

7.2

Y93E

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

427

28,955,773

ttCK

T. thermophilus HB8

Cytidine

0.236

1.4

5.8

25°C

7.2

Y93D

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

428

28,955,773

ttCK

T. thermophilus HB8

Uridine

null

0.056

25°C

7.2

Y93D

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

429

28,955,773

hsUCK2

Homo sapiens

Cytidine

0.138

6.4

47

25°C

7.2

H117Y

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

430

28,955,773

hsUCK2

Homo sapiens

Uridine

null

0.96

25°C

7.2

H117Y

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

431

28,955,773

hsUCK2

Homo sapiens

Cytidine

0.042

4.3

100

25°C

7.2

WT

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

432

28,955,773

hsUCK2

Homo sapiens

Uridine

0.058

5.2

90

25°C

7.2

WT

20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2，100–200 μM ATP, 15 μM NADH, 15 μM phosphoenolpyruvate, 0.5 mU of lactate dehydrogenase, 0.5 mU of pyruvate kinase

Table

433

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

0.28

51

182

80°C

7.5

natural WT

null

Table

434

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

0.33

48

146

80°C

7.5

recombinant WT

null

Table

435

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

0.31

2.2

7.2

80°C

7.5

Y253F

null

Table

436

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

null

0

50 or 80 °C

7.5

Y253W

null

Table

437

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

null

0

50 or 80 °C

7.5

Y253A

null

Table

438

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

0.17

3.6

21

80°C

7.5

P254G

null

Table

439

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

0.28

5.7

20

80°C

7.5

I255L

null

Table

440

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

0.13

3.2

25

80°C

7.5

I255S

null

Table

441

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

0.25

6

24

80°C

7.5

I255V

null

Table

442

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

0.19

7.9

41

80°C

7.5

I255M

null

Table

443

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

0.11

4.1

38

80°C

7.5

T256S

null

Table

444

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

0.14

4.3

31

80°C

7.5

T256A

null

Table

445

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

0.17

4.6

28

80°C

7.5

T256V

null

Table

446

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

null

0

50 or 80 °C

7.5

P257G

null

Table

447

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

null

0

50 or 80 °C

7.5

P257A

null

Table

448

11,683,888

OFOR

Sulfolobus sp. strain 7

Pyruvate

null

0

50 or 80 °C

7.5

P257V

null

Table