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Activation of the ADE Genes Requires the Chromatin Remodeling Complexes SAGA and SWI/SNF Rebecca N. Koehler, Nicole Rachfall, Ronda J. Rolfes Rebecca N. Koehler Department of Biology, Georgetown University, Washington, DC Nicole Rachfall Ronda J. Rolfes For correspondence: rolfesr@georgetown.edu The activation of the ADE regulon genes requires the pair of transcription factors Bas1 and Pho2. In a genome-wide screen for additional regulators of the pathway, strains with mutations in multiple subunits of the chromatin remodeling complexes SAGA and SWI/SNF were uncovered. These mutants exhibited decreased expression of an ADE5,7-lacZ reporter and native ADE compared to the wild-type strains, but the expression of the BAS1 and PHO2 genes was not substantially decreased. An unregulated Bas1-Pho2 fusion protein depended upon SAGA and SWI/SNF activity to promote transcription of a reporter. A significant but low-level association of Gcn5-myc and Snf2-myc with the ADE5,7 promoter was independent of adenine growth conditions and independent of the presence of the activator proteins Bas1 and Pho2. However, the increase in occupancy of Bas1 and Pho2 at ADE5,7 depended on both SAGA and SWI/SNF. The loss of catalytic activity of both SAGA and SWI/SNF complexes in the gcn5Δ snf2Δ double mutant was severely detrimental to ADE-lacZ reporter expression and native ADE gene expression, indicating complementary roles for these complexes. We conclude that Bas1 and Pho2 do not recruit the SAGA and SWI/SNF complexes to the ADE5,7 promoter but that the remodeling complexes are necessary to increase the binding of Bas1 and Pho2 in response to the adenine regulatory signal. Our data support the model that the SAGA and SWI/SNF complexes engage in global surveillance that is necessary for the specific response by Bas1 and Pho2. The ADE genes of Saccharomyces cerevisiae encode the enzymes of the purine nucleotide biosynthetic pathway responsible for the conversion of 5′-phospho-d-ribosyl-1(β)-pyrophosphate into purine nucleotides. Synthesis of purine nucleotides is metabolically regulated by feedback inhibition of the first step of the purine biosynthetic pathway by the end products of the pathway ATP and ADP (39). At the genetic level, ADE gene expression is transcriptionally repressed when cells are cultured in the presence of purine bases such as adenine (3, 4, 7, 8, 23, 54). Linking the genetic and metabolic regulation are the intermediates of the biosynthetic pathway SAICAR [1-(5′-phosphoribosyl)-4-(N-succinocarboxamide)-5-amino imidazole] and AICAR [1-(5′-phosphoribosyl)-4-carboxamide-5-aminoimidazole]. Cells with a mutation in any step of the pathway prior to SAICAR synthesis exhibit low, nonderepressible expression of ADE genes, whereas cells with a mutation in the steps after SAICAR or AICAR synthesis have high, constitutive ADE gene expression (39). Therefore, the regulatory signals for low levels of purine nucleotides are the pathway intermediates SAICAR and AICAR (39, 40). Nine ADE genes are up-regulated by the transcription factors Bas1 and Pho2 under derepressing conditions (3, 58, 65). These two factors also regulate the HIS1, HIS4, and HIS7 genes of the histidine biosynthesis pathway and GLN1, SHM2, and MTD1 genes involved in the synthesis of glutamine, glycine, and 10-formyl tetrahydrofolate, respectively (1, 4, 5). Together these 15 genes constitute the ADE regulon, although two recent studies have identified up to 56 genes that Bas1 may bind and regulate (21, 24). Both factors are necessary to stimulate expression, but their DNA binding is differentially regulated. Bas1 binds the ADE upstream activation sequence (UASADE) under both repressing and derepressing conditions (51), but Pho2 binds only under derepressing conditions and this binding depends upon interaction with Bas1 (12, 36, 51). The current model for adenine regulation is that the interaction between Bas1 and Pho2 is promoted by the pathway intermediates SAICAR and AICAR (39, 40, 65); this interaction stabilizes Pho2, with its own activation domain, at the UASADE and unmasks the latent activation domain in Bas1 (12, 36, 65), both of which are necessary to fully activate transcription. Although Bas1 and Pho2 are required to stimulate ADE regulon expression, how they interact with the transcriptional machinery or chromatin remodeling complexes is not known. At HIS7, Bas1 and Pho2 are thought to promote transcription through Gcn5-dependent histone acetylation (59). PHO5 expression, which is regulated by Pho2 in conjunction with Pho4, is dependent on the Snf2 and Gcn5 subunits of the SWI/SNF and SAGA complexes (27, 52). It has also been shown that Pho2 contacts the chromatin remodeling complex NuA4 and that this interaction is necessary for the expression of PHO5 (29). Thus, several chromatin remodeling complexes may be able to interact with Pho2 and Bas1 in different transcriptional contexts and therefore be relevant as additional regulators of the ADE regulon. In this study, we follow up on an observation that strains with mutations in several subunits of the SAGA and SWI/SNF chromatin remodeling complexes are sensitive to the adenine analog 4-aminopyrazolo-(3,4d)-pyrimidine (4-APP) (R. J. Rolfes et al., unpublished data). Previous studies of yeast demonstrated that resistance to 4-APP occurs in two different ways. Cells acquired a mutation in the purine-cytosine permease that blocked uptake of the analog (33, 35). Alternatively, cells acquired a specific mutation in the ADE4 gene that produced a feedback-insensitive form of the first enzyme in the purine nucleotide pathway (33-35). These results indicate that a critical balance between de novo synthesis of purine nucleotides and the intracellular concentration of analog-containing nucleotides is necessary for cells to grow on a medium containing 4-APP. Perturbing this balance can lead to 4-APP resistance or sensitivity (Rolfes et al., unpublished). We found that strains that carry mutations in SAGA and SWI/SNF are more sensitive to 4-APP, suggesting a role for these chromatin remodeling complexes in nucleotide metabolism. SAGA is a multiprotein complex whose Gcn5 subunit encodes a histone acetyltransferase (HAT). Gcn5 acetylates several lysine residues on the histone tails, including K14 on H3 and K8 and K16 on H4 (19). SAGA is also composed of adaptor proteins of the Ada family, TATA binding protein-associated factors, and Spt proteins involved in the structural integrity of the complex (53, 62). The SWI/SNF complex is a highly conserved remodeling complex that utilizes the energy from its ATPase component Snf2 to remodel the local chromatin structure (26, 31, 50). The recruitment of the HAT complex SAGA has been associated with recruitment of the SWI/SNF complex in many instances (10, 13, 14, 57). In some cases, acetylation by the SAGA component Gcn5 has been shown to increase retention of the SWI/SNF complex on promoters (13). Thus, it was significant that these two complexes were both identified in our screen, as they may carry out their actions in concert at ADE gene promoters. In this report we find that SAGA and SWI/SNF complexes associate with the ADE5,7 promoter, independently of adenine regulation and independently of Bas1 and Pho2. On the other hand, the Gcn5 subunit of SAGA and the Snf2 component of SWI/SNF are required for efficient binding of Bas1 and Pho2 to the UASADE5,7 under derepressing conditions but not under the basal (repressing) conditions. These results demonstrate a role for SAGA and SWI/SNF in increasing the occupancy of the transcriptional activators in response to the adenine regulatory signal. Yeast strains.All strains used in this study are described in Table 1. The homozygous diploid knockout collection was obtained from Research Genetics. To ensure that the strains carried the indicated deletion mutation, each strain selected from the knockout collection for study was analyzed by PCR amplification of the locus (data not shown) using the primers pairs listed on the Saccharomyces Genome Deletion Project website (http://sequence-www.stanford.edu/group/yeast_deletion_project/deletions3.html ). We obtained strains FY1352, FY1353, FY1354, and FY1360 from Fred Winston (41) and strains HQY367 and HQY453 from Alan Hinnebusch (38, 64). Strain RR439 is a derivative of FY1360 that was constructed to convert the snf2Δ::LEU2 allele to snf2Δ::leu2::kanMX by transformation with BamHI-restricted plasmid m3926 (61). Transformants were selected for G418 resistance and screened for leucine auxotrophy. Yeast strains Strains RR433, RR434, and RR435 are derivatives of FY1353, FY1354, and FY1360, respectively, that carry the BAS1-MYC13 allele, and strains RR430, RR431, and RR432 are derivatives of FY1353, FY1354, and FY1360, respectively, that carry the PHO2-HA3 allele (51). These strains were created by homologous recombination to insert the epitope tag into the chromosomal genes (51). The BAS1-MYC13 module was amplified from genomic DNA of strain RR376 using oligonucleotides JO-205 and JO-206, and the PHO2-HA3 module was amplified from genomic DNA of strain RR409 using oligonucleotides IO-611 and IO-612, as described previously (51). Transformants were selected on yeast extract-peptone-dextrose plates containing 200 μg/ml G418. PCR amplification of the loci from genomic DNA was used to confirm the presence of the tag (data not shown). Expression of the epitope-tagged protein was assessed by immunoblot analysis using anti-myc and antihemagglutinin (anti-HA) antibodies (data not shown). Strains RR436, RR437, RR440, RR441, and RR442 are derivatives of FY1353, FY1354, RR439, HQY367, and HQY453, respectively, that carry the bas1-10Δ::hisG allele. These strains were generated by replacement of the BAS1 locus with bas1-10Δ::hisG-URA3-hisG using the EcoRI-SphI fragment obtained from pR166 and selection on SC medium lacking uracil (51). The URA3 gene was popped out by culturing the strain on 5-fluoroorotic acid (5-FOA) medium to generate bas1-10Δ::hisG. The presence of the bas1 deletion allele was confirmed by PCR analysis of the genomic locus and by assaying adenine bradytrophy (data not shown). Strains RR443 and RR444 are derivatives of HQY367 and HQY453, respectively, that carry the pho2-10Δ::hisG allele. These strains were generated by integrative transformation with the EcoRI-SphI fragment obtained from pR167 and selection on SC medium lacking uracil (65). The URA3 gene was popped out by culturing these strains on 5-FOA medium to generate pho2-10Δ::hisG. The presence of the pho2 deletion allele was confirmed by the inability to grow on medium lacking inorganic phosphate and by PCR analysis of the genomic locus (data not shown). Plasmids.Plasmid pR109 is a fusion of the lacZ gene to ADE4 at codon 71 and was created by inserting lacZ into the ADE4 gene (65) and subcloning it into a LEU2 CEN6 vector (48) (plasmid construction details available upon request). Plasmid pR115 carries the ADE5,7-lacZ reporter, was constructed by inserting the lacZ gene into the ADE5,7 open reading frame (ORF) and then moving the fusion gene into a LEU2 CEN6 vector, and was described previously (65). Plasmids pR109 and pR115 are similar in that they have large genomic fragment inserts that carry the entire coding region and the entire promoter and regulatory region, extending into the flanking genes. Plasmid pR133, also described previously (65), carries only a small portion (139 bp) of ADE5,7 that carries the regulatory region (UASADE5,7) that was inserted into a heterologous system—the CYC1 core promoter driving the lacZ reporter—developed by L. Guarente and colleagues to characterize UAS elements (11). To distinguish these two types of lacZ reporters, we refer to this latter type as a UASADE5,7-lacZ reporter and the former type as an ADE-lacZ reporter. Plasmids pR166 (51) and pR167 (65) carry the bas1-10Δ and pho2-10Δ alleles, respectively. Plasmid m3926 converts LEU2 to leu2::kanMX through a simple insertion (61). Plasmid pCB286 (1) expresses full-length Bas1, and plasmid B273, obtained from Odd Stokke Gabrielsen, expresses the Bas1-Pho2 fusion protein (36). Media.Strains were cultured at 30°C on YPAD solid medium or SD and SC liquid or solid medium containing 2% glucose, 0.17% yeast nitrogen base (Difco), and 0.5% ammonium sulfate supplemented with amino acids as previously described (47). Adenine was added to a final concentration of 0.15 mM where indicated, and 4-APP was added to a final concentration of 75 μM. Medium lacking inorganic phosphate was prepared by precipitation of magnesium phosphate under alkaline conditions, as described previously (45). Medium containing 5-FOA was made by supplementing SC medium with 0.1% 5-FOA. G418 was added to YPAD medium at 200 μg/ml. β-Galactosidase assays.β-Galactosidase enzyme assays were performed as previously described (22). Briefly, cells were grown overnight in SC medium containing adenine. Cultures were diluted 1:50 into 50 ml of SC medium with adenine or SC medium lacking adenine and grown for 5 h with rotary agitation at 30°C. Extracts were prepared from pelleted cells by using glass beads, and β-galactosidase activities were measured from clarified whole-cell extracts (22). 4-APP spot test.Cultures were grown overnight at 30°C overnight in 5 ml of YPAD medium. Cultures were diluted to an optical density at 600 nm (OD600) of 0.2 and allowed to grow for approximately 3 hours. Approximately 1 ml of cells at an OD600 of 0.5 was pelleted and resuspended in 500 μl of sterile water. Serial 10-fold dilutions were prepared, and 5 μl of each dilution was spotted onto the surface of solid medium. Growth was assessed after 48 h of incubation at 30°C. RNA isolation and Northern blot assays.Cell cultures were grown overnight in SC medium with adenine. Cultures were diluted to an OD600 of 0.1 in 50 ml and were allowed to grow to an OD600 of 0.5. For derepression, cultures were pelleted, washed twice with SD medium, and resuspended in 50 ml of medium lacking adenine. After 30 min, the cells were pelleted and RNA was isolated using the RNA Isolation Kit (Epicenter); preliminary experiments showed that maximal expression of the ADE5,7 and ADE17 loci occurred at 30 min in the wild-type strain (data not shown). RNA samples were separated on 1.2% formaldehyde gels, blotted to GeneScreen Plus filters, and hybridized to probes (46). The ADE5,7 probe was prepared as described previously (43). Fragments for probes for BAS1, ACT1, and ADE17 detection were derived from PCR amplification of genomic DNA using the following oligonucleotides: BAS1-up (5′-CATGGGCCTCACTGGCTGAG) and BAS1-down (5′-ATCGCTATCCATGTTGTCC), ACT-up (5′-CTTTCTGGAGGAGCAATGATC-3′) and ACT-down (5′-GGATTCCGGTGATGGTGTTAC-3′), and ADE17-up (5′-GAGCAGCTGCTAAGAACCATG-3′) and ADE17-down (5′-ATGGCCAACGCCTCAGGCTC-3′). Radiolabeled probes were prepared using the Random Primed DNA Labeling Kit (Roche) incorporating [α-32P]dATP (43). RNA transcript levels were quantified by phosphorimaging analysis. ChIP.Chromatin immunoprecipitation (ChIP) assays were performed as described in reference 9, with the following changes. We added an additional wash of the lysed pellet using fresh FA lysis buffer. DNA in the extracts was fragmented by sonication using a Misonex 3000 sonicator for 30 s on and 30 s off for a total time of 14 min to produce chromatin fragments with an average size of 500 bp, as detected by gel electrophoresis and ethidium bromide staining. Protein G-Dynabeads (Dynal), approximately 2 × 107 in 50 μl, were preincubated with 2 μg of the anti-myc (Santa Cruz) or the anti-HA (Roche) antibody for 3 h at 4°C. Quantitative, multiplex PCR amplification mixtures contained 1× Platinum Taq polymerase buffer (Invitrogen), 1.5 mM MgCl2, 0.2 mM of each deoxynucleoside triphosphate, 1.6 μCi of [α-32P]dATP (Amersham), 0.2 μM POL1 primer pair, 0.2 μM ADE5,7 primer pair, 1.5 U of Platinum Taq polymerase (Invitrogen), and 2 μl of the immunoprecipitated chromatin sample or diluted input DNA (previously determined to be in the linear range for amplification) in a 15-μl reaction mixture volume. PCR parameters were 94°C for 4 min; 94°C for 30 s, 52°C for 30 s, and 65°C for 1 min for 26 cycles; and then 65°C for 5 min. PCR products were resolved on 6% polyacrylamide gels and quantified by phosphorimaging analysis. The sequences of the primer pairs were previously described for POL1 (56) and ADE5,7 (51). SAGA and SWI/SNF components are important for resistance to adenine analog 4-APP.In a screen of the yeast deletion collection with the adenine analog 4-APP, we identified ∼50 analog-sensitive mutant strains (Rolfes et al., unpublished). The mutants were divided into functional classes. One class contained genes involved in transcription, including BAS1 and PHO2, which are necessary for expression of the adenylate biosynthetic genes (reviewed in reference 42). This class of mutants also included the genes ADA1, ADA3, GCN5, SPT20, and SPT8, which encode subunits of the SAGA complex, and SNF2, SNF5, and SWI3, which encode subunits of the SWI/SNF complex. Because we identified multiple subunits from both complexes, we hypothesized that these complexes are regulators of ADE gene expression. To confirm and extend this initial observation, we assessed the 4-APP sensitivity of strains carrying mutations in all available identified subunits of the SAGA and SWI/SNF complexes. As shown in Fig. 1, we found that these eight originally identified strains were sensitive to 4-APP, as indicated by decreased growth of the mutants on SC medium containing 4-APP compared to the growth of the wild-type strain. We also observed sensitivity to 4-APP in five additional strains that carried deletion alleles of the genes SPT3, ADA2, SNF11, TAF14, and SNF6. These 13 mutations encode the majority of the nonessential subunits of the SAGA and SWI/SNF complexes (49, 62). SAGA and SWI/SNF mutant strains are sensitive to the adenine analog 4-APP. Strains with deletion mutations in the SAGA and SWI/SNF complexes were grown to equivalent cell densities, serially diluted (1:10), and spotted on complete medium containing 4-APP, containing adenine, or lacking adenine. Strains were allowed to grow for approximately 48 h and were photographed. We observed that the sensitivity to 4-APP varied in the different mutant strains. Some of the strains with mutations in the SAGA components exhibited strong sensitivity to 4-APP, including the spt3Δ and spt8Δ mutants, whereas others, like the ada3Δ strain, exhibited only weak sensitivity to 4-APP. Some of the strains had a secondary slow-growth phenotype and an additional 4-APP sensitivity that was very strong for the gcn5Δ mutant and weak for the spt20Δ, ada1Δ, and ada2Δ strains. Strains carrying mutations in the other identified SAGA subunit genes, UBP8, SGF73, and SGF11 (17, 37), were also tested for a 4-APP sensitivity phenotype; however, these strains showed no appreciable sensitivity to 4-APP (Fig. 1). Likewise, we observed a range of sensitivities to 4-APP in strains with mutations in different components of the SWI/SNF complex. Strong 4-APP sensitivity was detected in the snf2Δ, snf5Δ, and snf6Δ mutant strains, whereas the snf11Δ and taf14Δ mutant strains exhibited only a weak sensitivity to 4-APP. A strain with the swi3Δ mutation was also extremely sensitive to 4-APP, although it was severely growth compromised even on SC medium containing adenine. Together these results indicated that the absence of catalytic activity found in Gcn5 and Snf2, as well as the loss of key structural subunits provided by Ada1 and Spt20, led to the greatest degree of 4-APP sensitivity. Interestingly, we did not identify subunits from other remodeling complexes such as NuA3, ISWI, or Ino80. These findings indicate that the SAGA and SWI/SNF complexes play a critical role in ADE gene expression. SAGA and SWI/SNF mutants have decreased ADE gene reporter expression.To determine if mutations in SAGA and SWI/SNF affect ADE gene expression, we assayed expression of a lacZ reporter in strains carrying deletion mutations for subunits of the SAGA and SWI/SNF complexes. This lacZ reporter contains binding sites for Bas1 and Pho2 from the ADE5,7 promoter (UASADE5,7) in a heterologous context (CYC1-lacZ). This plasmid was chosen because the insert was extensively characterized by mutational analysis as the minimum necessary for regulation and because it is strongly derepressed by adenine limitation (44). Strains with mutations in subunits of SWI/SNF (snf2Δ, snf11Δ, and taf14Δ) and SAGA (gcn5Δ, spt3Δ, spt8Δ, and ada3Δ) were transformed with this reporter and assayed for effects on expression. We were unable to obtain stable transformants for several of the mutant strains after multiple attempts (swi3Δ, snf5Δ, snf6Δ, ada2Δ, ada1Δ, and spt20Δ strains). As shown in Fig. 2, we found that each mutant strain was unable to stimulate expression of the UASADE5,7-lacZ reporter to the level of the wild type under derepressing conditions (SC medium lacking adenine). The greatest effect on β-galactosidase activity was observed in the ada3Δ, snf11Δ, spt3Δ, and taf14Δ strains, with expression at approximately 20 to 41% of the wild-type activity. Expression of the reporter was also significantly affected in the snf2Δ, gcn5Δ, and spt8Δ mutant strains, in which β-galactosidase levels decreased to about 56 to 65% of the wild-type level. All of the mutants, with the possible exception of the taf14Δ mutant, responded to adenine limitation by increasing expression of the reporter gene; however, the extent of the derepression in each mutant strain was smaller than that found in the wild type. This observation indicates that the mutant strains were able to generate and receive the intracellular derepression signal to stimulate transcription of the ADE genes but were unable to fully initiate transcription. Expression of a UASADE5,7-lacZ reporter is compromised in SAGA and SWI/SNF mutants. The wild-type strain (BY4741/BY4742) and isogenic strains with mutations in subunits of SWI/SNF (snf2Δ, snf11Δ, and taf14Δ strains) and SAGA (gcn5Δ, spt3Δ, spt8Δ, and ada3Δ strains) were transformed with plasmid pR133 (44), which carries the UASADE5,7-lacZ reporter. Strains were grown in SC medium containing adenine (open bars) or lacking adenine (filled bars). Extracts were prepared from cell pellets, and β-galactosidase activities were measured in duplicate assays on at least three individual isolates. Error bars indicate standard deviations. Expression of ADE genes is dependent on SAGA and SWI/SNF.We have shown that SAGA and SWI/SNF components are necessary for efficient lacZ reporter expression driven by the ADE5,7 promoter and regulatory region. To investigate the involvement of SAGA and SWI/SNF in ADE gene expression, we assayed steady-state RNA levels expressed from the native loci in mutant and wild-type strains using Northern analysis. We chose to assay transcript levels from two well-characterized ADE genes, ADE5,7 and ADE17 (3, 39, 44), to represent the ADE regulon and to use the gcn5Δ and snf2Δ mutants defective in the catalytic activities found in each of the complexes. We found that the gcn5Δ and snf2Δ mutant strains were unable to derepress ADE5,7 and ADE17 to the wild-type levels (Fig. 3), and no effect was observed on the repressed levels. In the gcn5Δ mutant, expression of the ADE17 and ADE5,7 loci was reduced to 62 to 63% of the wild-type level, whereas in the snf2Δ mutant, expression was modestly reduced to 71 to 82% of the wild-type level. Thus, the SAGA and SWI/SNF chromatin remodeling complexes are required to achieve full expression of at least these members of the ADE regulon. Expression of ADE5,7 and ADE17 is reduced in gcn5Δ and snf2Δ mutants. RNA was prepared from wild-type (WT) (FY1353) and gcn5Δ (FY1354) and snf2Δ (FY1360) mutant strains that were harvested after 30 min of growth under repressing conditions (SC medium with adenine) and derepressing conditions (SC medium without adenine). RNA was blotted onto nylon membranes and hybridized to probes for ADE5,7, ADE17, and ACT1. Results were quantified by phosphorimaging analysis, and the levels of ADE5,7 and ADE17 were normalized to the reference ACT1 level. These results show that efficient transcription depends upon the SAGA and SWI/SNF remodeling complexes in addition to the Bas1 and Pho2 transcription factors. Several mechanisms could account for the decrease in the lacZ reporter and native gene expression that we detected in the mutants. The most likely mechanisms are that the Bas1 and Pho2 factors recruit the remodeling complexes that are necessary for the subsequent engagement of RNA polymerase II or that the remodeling complexes are necessary for the binding of Bas1, Pho2, or both to the UASADE. Alternatively, an indirect mechanism might be used if the expression of the activator genes BAS1 and PHO2 or the synthesis of the signaling molecules SAICAR and AICAR depends on the remodeling complexes. We experimentally address these possibilities below. SAGA and SWI/SNF mutations do not affect BAS1 or PHO2 expression.If expression of the BAS1 or PHO2 genes strongly depended on the SAGA and SWI/SNF complexes, then the levels of the activator proteins might be lower in the mutants, decreasing ADE gene expression. To determine if the expression of BAS1 was decreased in SAGA and SWI/SNF mutants, we performed Northern analysis on RNA isolated from strains carrying mutations in the genes encoding structural subunits (spt20Δ and snf5Δ) and catalytic subunits (gcn5Δ and snf2Δ) of both complexes. As shown in Fig. 4, we found that strains carrying the deletion mutations were not significantly affected in the transcription of BAS1. We also observed that the expression of BAS1 was not dependent on exogenous adenine in both the mutant strains and the wild-type strain, consistent with a previous report (65). Expression of BAS1 is not affected in strains with mutations in SAGA and SWI/SNF. (A) The isogenic wild-type (WT) strain and strains carrying mutations in the gcn5 and spt20 genes of SAGA and the snf2 and snf5 genes of SWI/SNF were grown in SC medium containing adenine and SC medium lacking adenine. Total RNA was purified from harvested cells, blotted onto nylon membranes, and hybridized to radiolabeled probes for BAS1 and ACT1. This experiment was performed twice, and results from a representative Northern analysis are shown. (B) The results were quantified by phosphorimaging analysis, and the BAS1/ACT1 ratio was normalized to the wild-type ACT1 level. Samples were analyzed in duplicate, and the error bars indicate the ranges. Open bars, SC medium containing adenine; filled bars, SC medium lacking adenine. Previous reports demonstrated that PHO2 expression is not affected by mutations in the SPT7 and GCN5 genes of SAGA or in the SNF2 and SWI1 subunits of SWI/SNF (28, 30, 55). We performed Northern analysis on RNA prepared from our strains and observed no difference in expression of PHO2 in gcn5Δ, spt20Δ, snf2Δ, and snf5Δ mutants (data not shown), in agreement with the previously published reports. Together, these results indicate that mutations in catalytic and structural subunits of SAGA and SWI/SNF do not substantially alter the expression of the genes encoding the transcription factors Bas1 and Pho2. SAGA and SWI/SNF mutants are defective in ADE expression with a constitutively active Bas1-Pho2 fusion protein.A second explanation for the decrease in ADE gene expression is that the derepression signals SAICAR and AICAR (39) are produced at lower levels in the mutants. Basal expression of the ADE genes is high in Saccharomyces (3, 39); thus, when feedback inhibition is initially relieved, basal levels of the pathway enzymes allow for immediate synthesis of downstream products, including the intracellular signals SAICAR and AICAR. If mutations in subunits of the SAGA and SWI/SNF decreased the basal expression of enzymes prior to formation of SAICAR and AICAR, then the ability to generate the derepression signal might be attenuated, leading to decreased reporter and native gene expression. The role of the regulatory signal is to promote interaction between DNA-bound Bas1 and Pho2 (36, 65). This interaction is critical for unmasking latent activation domains in Bas1. To circumvent the need for the signal, we used a Bas1-Pho2 fusion protein (36). This fusion protein constitutively stimulates expression of target genes and is independent of exogenous adenine levels and the intracellular signals (36). To prevent competition between native Bas1 and the Bas1-Pho2 fusion protein for binding sites, we deleted the BAS1 gene in the wild-type and remodeling mutant strains prior to transformation with plasmids that express Bas1 or the Bas1-Pho2 fusion protein and the lacZ reporters. Because the gene encoding the Bas1-Pho2 fusion protein was marked with URA3, we could not use plasmid pR133 as we did above but instead used two different lacZ reporters. The ADE4-lacZ and ADE5,7-lacZ reporters carry the core promoters and regulatory regions from the native ADE4 and ADE5,7 genes, and both reporters are carried on plasmids marked by LEU2. We then asked whether expression of the ADE-lacZ reporter genes was altered in the SAGA and SWI/SNF mutants. As shown in Fig. 5, in the absence of any Bas1 protein, we found only basal, nonderepressible expression of both the ADE5,7-lacZ and ADE4-lacZ reporters in all strains, whereas strains expressing Bas1 stimulated adenine-regulated expression in the wild-type strain and decreased expression in the remodeling mutants. As expected, the Bas1-Pho2 fusion was not repressible by adenine and it promoted higher reporter gene expression than the Bas1 and Pho2 proteins in the strain with wild-type remodeling complexes (36). Interestingly in the gcn5Δ and snf2Δ mutants, the Bas1-Pho2 fusion protein was able to express the ADE5,7-lacZ reporter to only 20 to 30% of the wild-type level and the ADE4-lacZ reporter to only about 10 to 20% of the wild-type level (Fig. 5A and B). Thus, engineering the transcriptional activators to be independent of the depression signal did not overcome the requirement for the remodeling complexes. These data are consistent with a model in which SAGA and SWI/SNF are involved in promoting transcription after reception of the regulatory signal by the activators, by making the chromatin template more accessible to the transcriptional machinery. However, these data are also consistent with the model that SAGA and SWI/SNF are involved in the binding of the activators to the UASADE, affecting Bas1-Pho2 fusion protein occupancy. Since these roles are not mutually exclusive, the remodelers may have both activities. A constitutively active Bas1-Pho2 fusion protein promotes only low-level expression of ADE-lacZ reporters in gcn5Δ and snf2Δ mutant strains. The wild type (RR436) and strains carrying mutations in gcn5 (RR437) and snf2 (RR439) were transformed with empty vector (YCp50), Bas1 (pR201), or the Bas1-Pho2 fusion (B273), as well as reporter plasmids for ADE5,7-lacZ (pR115 in panel A) or ADE4-lacZ (pR109 in panel B). Strains were grown in SC medium containing or lacking adenine. Extracts were prepared from cell pellets, and β-galactosidase activities were measured in duplicate assays for at least three individual isolates. SAGA and SWI/SNF are present at the ADE5,7 promoter in both repressing and derepressing conditions.The data presented above suggest that Bas1 and Pho2 are capable of recruiting the SAGA and SWI/SNF complexes. We wanted to determine whether we could detect the presence of these complexes at an ADE gene promoter. We performed ChIP assays, using a tagged subunit found in each remodeling complex, Spt7-myc for SAGA and Snf2-myc for SWI/SNF. These tagged strains were used by other researchers to investigate the association of SAGA and SWI/SNF with the ARG1 and SNZ1 genes (38, 64). We assayed binding of SAGA and SWI/SNF at the promoter of the ADE5,7 gene relative to their binding at the POL1 ORF and asked if their binding increased under the derepressing conditions of SC medium without adenine. We found that Spt7-myc and Snf2-myc are present at the promoter of the ADE5,7 gene independently of the presence or absence of adenine (Fig. 6A). Relative to the occupancy of Bas1-myc, we found lower association of both Spt7-myc and Snf2-myc, although in both cases we were able to chromatin immunoprecipitate the promoter fragment significantly more highly than in the no-tag control (NTC) (Fig. 6B). We also calculated the percent IP and observed a seven- to ninefold increase in Snf2-myc and Spt7-myc relative to the NTC strains, indicating their presence at the ADE5,7 promoter (Fig. 6C). Somewhat surprisingly, we found that both Spt7-myc and Snf2-myc were able to chromatin immunoprecipitate to POL1, indicating that SAGA and SWI/SNF are almost as equally abundant in the POL1 ORF as they are at ADE5,7 (Fig. 6A). Thus, in comparing the ratio of ChIP at ADE5,7 to that at POL1, we observed a ratio slightly above 1, at about 1.3 for each sample (Fig. 6B), indicating that SAGA and SWI/SNF were only slightly more likely to be found at the ADE5,7 promoter than at the POL1 ORF. A similar observation was made by Kim et al. (18), who showed that the ratio of the HIS3/POL1 ChIP signal was only slightly above 1. We conclude that both SAGA and SWI/SNF have low-level association with the ADE5,7 promoter that is not increased under derepressing conditions. SAGA and SWI/SNF occupy the ADE5,7 promoter independently of adenine. (A) ChIP assays were performed using antibodies to the myc tag on proteins Snf2, Spt7, and Bas1 along with an NTC strain, FY1353. A 500-fold dilution of the input control and the undiluted IP DNA were amplified by PCR by using primers specific for ADE5,7 and POL1 in the presence of [α-32P]dATP. Results from a representative ChIP are shown. The PCR products were resolved on 6% polyacrylamide gels, visualized, and quantified by phosphorimaging analysis. Enrichment (n-fold) was calculated as the ratios of the ADE5,7 signals in the IP-to-input samples and normalized for the corresponding ratios calculated for POL1 in the IP-to-input samples. (B) The resulting values from three ChIP experiments were plotted as the averages, with standard deviations, of the results from PCR amplifications of chromatin-immunoprecipitated DNA from three independent experiments. Strains grown with adenine are represented by open bars, and strains grown in medium lacking adenine are represented by filled bars. (C) Calculation of the percent IP was carried out by dividing the IP signal for ADE5,7 by the corresponding input signal. For all strains, values were significant, P ≤ 0.05, compared to the NTC. Bars are as defined for panel B. SAGA and SWI/SNF association with the ADE5,7 promoter is independent of Bas1 and Pho2.The association of SAGA and SWI/SNF at ADE5,7 might depend upon the transcriptional activator Bas1 or Pho2. We performed ChIP assays using an antibody to the myc tag on Spt7 and Snf2 in strains that carried null alleles of bas1 or pho2 (Fig. 7). Occupancy of SAGA and SWI/SNF was unchanged by the absence of either transcription factor. SAGA and SWI/SNF associate with the ADE5,7 promoter independently of the Bas1 and Pho2 activators. (A) ChIPs of Spt7-myc and Snf2-myc were performed in wild-type and bas1Δ mutant strains (RR441 and RR443) along with an NTC strain, FY1353. (B) Graphical representation of the ChIP results. The ChIP data were plotted, showing the averages and standard deviations of results from PCR amplifications of chromatin immunoprecipitated from three independent experiments and normalized to wild-type levels. (C) ChIPs of Spt7-myc and Snf2-myc were performed in wild-type and pho2Δ mutant strains (RR442 and RR444). (D) Graphical representation of the averages and standard deviations of data from PCR amplifications of chromatin immunoprecipitated from three independent experiments and normalized to wild-type levels. WT, wild type. For panels B and D, open and filled bars represent strains grown in medium with and without adenine, respectively. SAGA and SWI/SNF affect factor promoter occupancy of Bas1 and Pho2.We explored the possibility that SAGA and SWI/SNF affect the binding of Bas1 or Pho2 to UASADE, altering their occupancy. Previous work from our laboratory, using ChIP assays, showed that Bas1 binds several ADE promoters under repressing conditions and its occupancy increases by approximately 2.2-fold under derepressing conditions (51). We used ChIP analysis to look for the presence of the Bas1 protein at the promoter of the ADE5,7 gene when strains harbored the gcn5Δ and snf2Δ deletion alleles. Interestingly, we found that the absence of Gcn5 or Snf2 affects the binding of Bas1-myc to ADE5,7 (Fig. 8A). Under the repressing conditions of SC medium containing adenine, Bas1 is present at the promoter in the mutants at the same level as that found in the wild type. However, under derepressing conditions of SC medium lacking adenine, binding of Bas1 to the promoter is clearly reduced in the mutants (Fig. 8A). These results indicate that the absence of SAGA and SWI/SNF activity decreases Bas1 occupancy at ADE5,7. Promoter occupancy of Bas1 and Pho2 is decreased in SAGA and SWI/SNF mutants. (A) ChIPs of Bas1-myc were performed with wild-type (RR433), gcn5Δ (RR434), and snf2Δ (RR435) strains along with an NTC strain (FY1353). (B) Enrichment (n-fold) of Bas1 binding. ChIP assays were performed on strains grown with adenine (open bars) and without adenine (filled bars). PCR amplification from the ChIP experiments was quantified as the ratio of signal in the IP relative to input DNA, normalized to the POL1 signals. The average values from three independent ChIP experiments are plotted, with the standard deviations; mutants were significantly different from the wild type under derepressing conditions (P ≤ 0.05). (C) ChIPs of Pho2-HA were performed with wild-type (RR430), gcn5Δ (RR431), and snf2Δ (RR432) strains along with the NTC strain (FY1353). (D) Enrichment (n-fold) of Pho2 binding. ChIP analyses were prepared from strains grown with adenine (open bars) and without adenine (filled bars). PCR amplification from the ChIP experiments was quantified as the ratio of signal in the IP relative to input DNA, normalized to the POL1 signals. The average values from three independent ChIP experiments are plotted, with the standard deviations; mutants were significantly different from the wild type under derepressing conditions (P ≤ 0.05). Similar results were observed with the binding of Pho2 to the ADE5,7 promoter. We used ChIP analysis to look for the presence of the Pho2 protein at the promoter of the ADE5,7 gene when strains harbored the gcn5Δ and snf2Δ deletion alleles. We found that the absence of Gcn5 or Snf2 decreases the binding of Pho2-HA to ADE5,7 under derepressing conditions but not under repressing conditions (Fig. 8B). The reduction in Pho2 binding could be direct or indirect through the decreased binding of Bas1, as we have shown elsewhere that Pho2 is unable to efficiently bind to the promoter in the absence of Bas1 (51). However, clearly the occupancy of this protein is affected by the loss of Gcn5 and Snf2. Together, these results demonstrate a role for the SAGA and SWI/SNF complexes in facilitating wild-type levels of occupancy of the Bas1 and Pho2 transcription factors at the promoters under derepressing conditions but with little to no effect under repressing conditions. In the absence of the remodeling complexes, formation of the transcriptionally active Bas1-Pho2 heterodimer is reduced, with effects on the expression of the target ADE genes. However, SAGA and SWI/SNF are not apparently recruited by these activators. ADE gene regulation requires SAGA and SWI/SNF catalytic activity.Our results indicate that both SAGA and SWI/SNF are necessary for ADE gene transcription; however, disruption of either complex alone lowered but did not completely eliminate ADE gene expression. Therefore, we hypothesized that the loss of both Gcn5 and Snf2 would exacerbate the reduction of ADE gene expression below that observed in either of the single mutants. We obtained a strain carrying mutations in both gcn5 and snf2 from Fred Winston (41); the mutant showed that the combined loss of the two genes resulted in very poor growth even on rich medium. We examined the sensitivity of the gcn5Δ snf2Δ strain to the adenine analog 4-APP to determine if sensitivity was increased relative to the single mutant strains. As shown in Fig. 9, each of the single gcn5Δ and snf2Δ mutants in this new strain background showed significant sensitivity to 4-APP compared to the wild type. Interestingly, the double mutant strain was extremely sensitive to 4-APP, much more so than either of the single mutants and about as sensitive as the bas1Δ strain (Fig. 9A). This finding suggests additive roles of the SAGA and SWI/SNF remodeling activities at the ADE genes. gcn5 and snf2 are nonredundant for ADE transcription. (A) Wild-type, single mutant (gcn5Δ or snf2Δ), and double mutant (gcn5Δ snf2Δ) strains were grown to equivalent cell densities; serially diluted (1:10); spotted onto SC medium, SC medium containing 4-APP, and SC medium lacking adenine; and allowed to grow for approximately 48 h at 30°C. (B) The parental wild-type and the single and double mutant strains were transformed with a UASADE5,7-lacZ reporter (pR133). Strains were grown overnight in SC medium, inoculated into SC medium containing (open bars) or lacking (filled bars) adenine, and assayed for β-galactosidase activity. β-Galactosidase activities were measured in duplicate assays on at least three individual isolates. (C) Expression of ADE5,7 and ADE17 in parental wild-type, gcn5Δ and snf2Δ single mutant, and gcn5Δ snf2Δ double mutant strains was measured by Northern analysis. RNA was prepared from the four strains that had been grown in SC medium and transferred to SC medium lacking adenine. Expression was normalized to ACT1 transcript levels, and the experiment was repeated to confirm results. We confirmed that this sensitivity to 4-APP was correlated with a loss of expression of the UASADE5,7-lacZ reporter (65). We found that both single mutant strains in this background were unable to fully derepress transcription (Fig. 9B). The snf2Δ mutation reduced transcription to approximately 35% of that in the isogenic wild type, whereas the gcn5Δ mutation had a stronger effect, reducing transcription to 10% of the wild-type level. The effect on gene expression in this strain background was more severe than what we had detected in the Research Genetics diploid knockout strain (Fig. 2). Interestingly, transcription in the gcn5Δ snf2Δ double mutant was barely detectable and virtually the same as that found under the repressing condition. Thus, the loss of catalytic activity from both complexes eliminated the ability to derepress the ADE5,7 gene. We obtained the same result using alternate ADE5,7-lacZ and ADE4-lacZ reporters (pR109 and pR115; data not shown): loss of either catalytic subunit alone decreased expression under derepressing conditions, but the loss of both activities resulted in only basal expression and the elimination of derepression. To confirm the result from the lacZ reporter assays, we assessed expression from the chromosomal ADE5,7 and ADE17 loci in wild-type and mutant strains using Northern analysis. For each gene assayed, transcripts were barely detectable in double mutant strains (Fig. 9C). This result was consistent with the lacZ reporter expression. These results indicate that both the SAGA and SWI/SNF complexes are required for wild-type levels of ADE gene expression. Genome-wide studies using DNA microarrays have investigated the loss of activity of the SAGA and SWI/SNF complexes. In one study, Holstege and colleagues (15) investigated the loss of both complexes from cells grown in rich (yeast extract-peptone-dextrose) medium, a condition in which the ADE regulon is repressed. Not surprisingly, their data showed that there was little to no difference in expression of the ADE regulon genes in the gcn5 and snf2 mutants relative to the wild type under these repressing conditions. Sudarsanam and colleagues (55) compared gene expression from cells grown in minimal medium with that from cells grown in rich medium to identify additional genes dependent upon SWI/SNF. About 1% of the genome showed an effect of threefold or greater. A great many genes, including several ADE genes, had small differences of less than threefold in gene expression in the snf2 and swi1 mutants. While these expression effects are small, they might nonetheless be significant under certain growth conditions. In this report we demonstrate that the SAGA and SWI/SNF complexes are required for full expression of the ADE4, ADE5,7, and ADE17 genes, members of the ADE regulon. These remodeling complexes were identified by a genome-wide screen for 4-APP sensitivity, a phenotype exhibited by bas1Δ and pho2Δ mutants (Fig. 1) (Rolfes et al., unpublished), suggesting a role for the remodeling complexes in the expression of ADE genes. In some instances, the recruitment of the SAGA complex and its Gcn5 bromodomain have been shown to stabilize the SWI/SNF complex at promoter nucleosomes (13); thus, it was striking that the screen identified both the SAGA and SWI/SNF complexes. Indeed, the SAGA and SWI/SNF mutant strains exhibited decreased gene expression from several ADE genes in lacZ reporter and Northern assays (Fig. 2 and 3). Our data are consistent with the data from the Winston laboratory (55) in terms of the magnitude of the effect detected on transcription. Notably, however, we found that this difference in expression due to the loss of SAGA and SWI/SNF imposed a biological effect, namely, a lower tolerance for nucleotide pool imbalances. Our data also indicate that the SAGA and SWI/SNF complexes are performing nonredundant roles in ADE gene expression. The double deletion strain was unable to derepress expression to any significant level above basal expression at ADE5,7-lacZ (Fig. 9B) or at native ADE5,7 and ADE17 loci (Fig. 9C). Thus, activity from either SAGA or SWI/SNF is necessary for a minimum derepression but full expression required each complex. We conclude that these complexes are performing complementary roles in modifying and remodeling chromatin necessary for ADE gene expression. However, it is interesting that we observed virtually no 4-APP sensitivity in the ubp8, sgf11, and sgf73 mutant strains. This finding suggests that the activities performed by these components, such as deubiquitination, may not be critical for ADE gene expression. Alternatively, perhaps these subunits are not required for global surveillance (discussed below). Association of the Bas1 and Pho2 activators with UASADE5,7 requires chromatin remodelers.Many well-studied activators, such as Gcn4, have been shown to recruit the SAGA and SWI/SNF remodeling complexes during transcriptional activation (9). Less studied is the role of these complexes in the binding of transcription factors to their binding sites (32). We found that the SAGA complex (via Spt7-myc) and the SWI/SNF complex (via Snf2-myc) associate with the ADE5,7 promoter at a low but significant level that was surprisingly not affected by the SAICAR/AICAR regulatory signal or the presence of the activators Bas1 and Pho2. However, we found that an increase in Bas1 and Pho2 binding that occurs under derepressing conditions was dependent on SAGA and SWI/SNF. This result was strengthened by the observation that a constitutively active Bas1-Pho2 fusion protein also required the remodeling complexes. Our data show that the SAGA and SWI/SNF remodeling complexes are not recruited by the Bas1 or Pho2 activator, yet these remodelers associate with the ADE5,7 gene and their activity is important for the increased binding of the activators and for transcription. This finding agrees with models for the action of the remodeling complexes described by other investigators. Cosma et al. (2) demonstrated that binding of SBF (Swi4/Swi6) required the activity of both SAGA and SWI/SNF whereas the binding of Swi5 did not. The association of Pho4 with nucleosomal UAS2 at PHO5 also required both Snf2 and Gcn5, although Pho4 binding at UAS1 did not require these remodelers (6, 52). Interestingly, both Swi5 and Pho4 cooperate with Pho2, but neither of these previous studies examined effects on Pho2 binding. We found a dependence on both SAGA and SWI/SNF for the binding of Pho2. While Bas1, Pho4, and Swi5 can all bind to their UAS elements in the absence of the remodeling complexes (presumably once Pho4 and Swi5 become nucleus localized), only Bas1 requires the remodelers to respond to the regulatory signal. Both SAGA and SWI/SNF catalytic functions are important for ADE gene derepression.Our data point to a second role for the chromatin remodeling complexes in transcription. We found that transcription driven by the Bas1-Pho2 fusion protein depended on both Gcn5 and Snf2. Normally, Bas1 and Pho2 do not interact until the regulatory signal is generated; combining the genes to produce the fusion protein makes reception of the signal moot. The Bas1-Pho2 fusion protein promotes transcription constitutively at the ADE1 (36) and ADE5,7 and ADE4 (Fig. 5) genes, indicating that it is constitutively bound to DNA, and yet it still requires the remodeling activities from both SAGA and SWI/SNF. Since a change in promoter occupancy under these conditions is unlikely, our data suggest that SAGA and SWI/SNF are involved at events downstream of factor binding, possibly remodeling chromatin for the binding of the general transcription factors or for transcriptional elongation. Further study will be necessary to define any additional roles. Global surveillance.How might the SAGA and SWI/SNF complexes associate with the ADE5,7 promoter since they are not recruited by Bas1 and Pho2? One possibility is that the complexes are recruited by another factor. Dhasarathy and Kladde (6) showed that myc-tagged Snf2 and Gcn5 are recruited to RPS22B by Abf1, even though the remodelers are not required for expression of this locus. It is possible that Abf1, which binds to the ADE5,7 promoter (44), recruits SAGA and SWI/SNF. However, since Abf1 is found only in the ADE5,7 promoter and not in the promoters for other ADE genes such as ADE4 and ADE17 (25, 44, 63), this putative mechanism would not explain the transcriptional requirement for SAGA and SWI/SNF at these other ADE genes. Perhaps other factors not studied in this report facilitate or enhance SWI/SNF and SAGA association, as was observed for Mediator at the ARG1 promoter (64). Another explanation for the presence of SAGA and SWI/SNF at promoters is global surveillance. Through the global acetylation mechanism, HATs enzymatically modify histone proteins within large chromatin domains, including both coding sequences and intergenic regions, without any apparent DNA sequence specificity or specific recruitment. Kuo et al. (20) demonstrated a global role for the activity of the Gcn5 HAT. They showed that the level of H3 acetylation at many genes, including ADE5,7, was decreased in a gcn5 mutant, relative to the wild type, when the Gcn5-containing complexes were not specifically targeted to these promoters. Other researchers have also described a similar phenomenon for the Gcn5 HAT (16, 20, 60). Our data are consistent with this global role, not only for Gcn5-containing complexes but also for the first time for the SWI/SNF complex. Thus, it is possible that a global surveillance role, while not fully understood, could explain the dependence on these chromatin remodeling complexes for ADE gene transcription. SAGA and SWI/SNF function as coactivators in ADE gene derepression.Our current model is that the presence of SAGA and SWI/SNF at the promoter facilitates efficient binding of the activators, Bas1 and Pho2, and thereby they function as coactivators in the derepression of ADE genes. SAGA and SWI/SNF are associated with ADE gene promoters as a part of their global surveillance role; we propose that they are present to efficiently and quickly turn on the ADE genes upon reception of the regulatory signal. There are other remodeling complexes previously shown to be poised under repressive conditions. NuA4, necessary for PHO5 activation and chromatin remodeling, is critical for chromatin remodeling even prior to activation, demonstrating that NuA4 readies or poises the PHO5 for activation (29). NuA4 is also present under repressing conditions, and, surprisingly, no increase in its association with promoters was detected upon induction. Interestingly, NuA4 was not actually required once the PHO5 gene was activated; Nourani et al. proposed that targeted hyperacetylation of nucleosomal histones under repressive conditions prepared the promoter and potentiated a rapid remodeling step, followed by transcriptional induction in response to signals (29). Other researchers have proposed that chromatin remodelers are continuously required because there is rapid, dynamic equilibrium between active and repressive chromatin structures (6). This is a possibility for SAGA and SWI/SNF involvement in ADE gene activation as well, where ADE genes need to be turned on quickly and efficiently. This "poising" and the continuous presence of the complexes at low levels of binding, as suggested by other researchers (29), are more efficient for stimulating gene expression quickly and thus rapidly producing biosynthetic products. SAGA and SWI/SNF may therefore associate and be poised at low levels to facilitate the first round of transcription and the increase in factor occupancy. We also cannot rule out the possibility that SAGA and SWI/SNF association will not increase at later time points after induction, not detected at the initial rounds of transcription as well. Interestingly, Dhasarathy and Kladde observed that as late as 12 h after induction the occupancy of the activator Pho4 and the coactivators SAGA and SWI/SNF at the PHO5 promoters reached the maximum (6). How this increased binding at later times would correlate with gene expression is not inherently clear. Remodelers might be required not only for initial opening of chromatin but also for the long-term stabilization of the preinitiation complex, allowing for repeated RNA polymerase II loading. This work was supported by MCB-0344371 from the National Science Foundation and institutional support from the Graduate School of Arts and Sciences of Georgetown University. We are very grateful to Fred Winston for providing yeast strains FY1352, FY1353, FY1354, and FY1360. We also thank the Hinnebusch lab for HQY367 and HQY453 as well as Chhabi Govind for his assistance with ChIP assays. We thank David Clark for myc antibody and protein G-Dynabeads. 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"Chasselas - Féchy to Dézaley" is a superb book about wine, it also offers traditional recipes of Vaud, easy to prepare and which go perfectly with the Chasselas. Most of the illustrations in this book come from Paul Boesch's book "La Grande Année Vigneronne", published in 1935 by the editions of Verseau, Roth & Sauter Print Center SA Denges. It consists of twelve woodcuts (black and white and color) illustrating the daily work during a year in the vineyard of Vaud. The current photographs of the vineyard are made by photographer Patricia von Ah. This magnificent book presents 99 Chasselas, classified by region. He proposes for each wine, the tasting notes, the technical data and the portrait of the estate. Every generation has laid down the culture of Chasselas. A relationship to discover rooted in a long history. This book is a beautiful tribute to Chasselas and the successive evolutions of the work around this grape variety. This grape made the happiness for the table of the Ottoman sultans. At the table of François 1er its cluster crowned with luxurious meals. It fed for a long time the stories and the legends. Scientists, historians and wine growers lift the veil on the origin of this symbolic vine of French-speaking Switzerland. From Constantinople up to the vine arbour of the Roy of Fontainebleau, following the banks of the Loire, exploring the Burgundy and the vineyards of French-speaking Switzerland, the mystery of the chasselas grape shows itself at the rate of the epic. Never the camera had also approached near a grape, never vine had so much been sounded by the genetics. Of the pip to the Grand Cru, of the infinitesimal to the universal geography, the history of the chasselas grape as you have never seen it.

Andries Hendrik Potgieter (Graaff-Reinet, 19 de diciembre de 1792-Schoemansdal, 16 de diciembre de 1852) fue un líder voortrekker. Se desempeñó como el primer jefe de estado en Potchefstroom desde 1840 hasta 1845 y también como primer jefe de estado de Zoutpansberg entre 1845 y 1852. Potgieter nació en el distrito Tarkastad de la Colonia del Cabo, el segundo hijo de Petronella Margaretha y Hermanus Potgieter. Él creció para ser un rico granjero de ovejas y luchó con las Cuarta y Quinta Guerras Fronterizas. Sin embargo, como muchos otros granjeros holandeses, franceses, y descendientes de alemanes conocidos como bóeres que vivían en la Colonia del Cabo, él decidió dejar la colonia en 1834. Retrasado por la Sexta Guerra Fronteriza, Potgieter y un grupo de Voortrekkers bajo su mando se marcharon en 1835. Otros emigrantes bajo el liderazgo de Johannes Hendrik Janse y Louis Trichardt Rensburg lo habían precedido. El líder espiritual de los voortrekkers, Sarel Arnoldus Cilliers, más tarde se unió al viaje de Potgieter. Él y sus emigrantes se movieron al interior del luego Estado Libre de Orange, donde firmaron un tratado con el líder del Baralong, Moroka. El tratado estipuló que Potgieter protegería al Baralong contra los atacantes matabele a cambio de la tierra. La extensión de tierra cedida era del río Vet al río Vaal. El líder matabele, Mzilikazi, amenazado por la incursión blanca en lo que él veía como su esfera de la influencia, condujo al ataque Matabele al laager (círculo de carretas de bueyes) de Potgieter en octubre de 1836 en Vegkop, cerca de la ciudad actual de Heilbron. El ataque fue rechazado, pero los Matabele se marcharon con la mayor parte de los bueyes trekker, animales de tiro cruciales para sus carros. Los grupos de viajeros combinados de Piet Retief y Gerrit Maritz vinieron al rescate de Potgieter. Moroka también ayudó con bueyes. Su grupo se unió con Retief y Maritz en Thaba Nchu, donde formaron un gobierno voortrekker y decidieron moverse a Natal. Potgieter no estuvo a favor de esto y se quedó en el Estado Libre en 1838. Luego de que Piet Retief y su grupo fueran matados por Dingane y los grupos Voortrekker fueran atacados en Bloukrans y el Río de los Bosquimanos, Potgieter y otro líder, Pieter Lafras Uys, fueron para ayudarlos. Una fuerza militar fue reunida, pero para prevenir el cisma y la discordia, el nuevo líder Voortrekker en Natal, Maritz, diplomáticamente el anunció que tanto Uys como Potgieter debían mandar. Una lucha surgió entre el impetuoso Uys y Potgieter. Esta fuerza dividida fue atraída en una emboscada por los zulús en Italeni y tanto Uys como su hijo Dirkie, fueron matados. La fuerza rodeada y superada en número huyó. Potgieter fue criticado por sus acciones y la fuerza fue llamada el Comando de la Huida o "Die Vlugkommado". Él fue acusado adelante, injustamente, de causar deliberadamente la muerte de Uys por conducir la fuerza a la emboscada. Él se marchó Natal para siempre pronto después y se trasladó al Transvaal. Posteriormente continuó para fundar Potchefstroom (llamada así por él) y se desempeñó como primer jefe de estado de la República de Potchefstroom entre 1840 y 1845. Potgieter más tarde también continuó fundando Ohrigstad (al principio se llamó Andries-Ohrigstad por Potgieter y George Ohrig) en 1845, como una estación de comercio. Debido a la malaria la ciudad tuvo que ser abandonada y los habitantes, incluso Potgieter, se trasladaron al área Soutpansberg, donde fundó la ciudad Soutpansbergdorp, que más tarde renombró como Schoemasdal. Después de la anexión de Natal en 1842 por Gran Bretaña, muchos trekkers de Natal se trasladaron al Estado Libre y Transvaal. Estos recién llegados y su líder, Andries Pretorius, rechazaron aceptar la autoridad de Potgieter, y surgió una lucha por el poder. La guerra fue prevenida, y en 1848 y fue firmado el tratado de paz de Rustenburg. Potgieter murió el 16 de diciembre de 1852 en Schoemasdal. Políticos de Sudáfrica del siglo XIX Historia de KwaZulu-Natal

Regional IT/BPM cities to spearhead inclusive growth in Sri Lanka In a move to create much-needed IT/BPM capacity required for the expansion of the industry in Sri Lanka, which is now poised for accelerated growth, SLASSCOM Vice Chairman Mano Sekaram is advocating the creation of regional second tier cities to bring about inclusive growth in the country. The IT/BPM industry is now amongst the top five largest export earners, with an estimated export revenue of US$ 720 million in 2013 and a growth of 33,700 employees in 2007 to 75,100 employees in 2013. The industry has also played a key role in developing ICT, which has played a critical role in the country's economic transformation and there is an urgent need to take ICT to all parts of the country in order to bring about national connectivity. The IT/BPM sector has been and will continue to be a driving force in this area. Sri Lanka is fast emerging as a niche global sourcing destination for IT and BPM services with a high future growth potential, with AT Kearney ranking Sri Lanka among the top 25 destinations in their global ranking. The worldwide addressable market for IT/BPM off-shoring has been estimated at $403 billion and the Sri Lankan industry's Vision 2022 aims it to become the number one foreign exchange earner of the country, with US$ 5 billion in revenue, creating 200,000 direct jobs and 1,000 start-ups. SLASSCOM Vice Chairman and 99X Technology CEO Mano Sekaram observed that growth has so far been 'Colombo-centric' and stressed on the need to bring about inclusive growth that will touch every segment of society. "The fastest way of doing this is through the spread of ICT in rural areas, through the creation of regional cities." He pointed out that the IT/BPM industry is one that can operate from any location, given that an enabling business environment and sound infrastructure are in place. "Our greatest untapped human resources rest in the regional areas which is where 80% of our current workforce comes from." Furthermore, given Sri Lanka's development drive, Colombo, which is already home to hundreds of IT/BPM firms, will only get more congested. Increased expenses would mean higher costs of operation for companies. "The creation of 'second tier cities' would see a paradigm shift from the Western Province and would empower regional systems. Rural people will be able to reap benefits while living in their own households, allowing them to enjoy a far better standard of life. This will assist in achieving the Government's vision of transitioning Sri Lanka into a middle-income country," Sekaram asserted. This shift will also see an enhancement of employment opportunities, by developing the capabilities of the youth of the country and making them employable in the industry. A readiness assessment of locations has shown that Jaffna, Kandy and Hambantota have potential to be developed as second tier cities in Sri Lanka so far, given the infrastructure already in place, connectivity, current and planned development, their capacity to generate skilled workforces. In line with this, SLASSCOM has also put forward a proposal for the acceleration of the regional IT/BPM hub concept to fast-forward the movement of businesses to these second tier cities by creating sufficient incentives for early movers to jumpstart the process, and has identified a number of key development areas to work on. « Norochcholai Lakvijaya Power Plant 2nd phase opening on Sept.16 Lalana Sawiya Women Trainee Programme in Gampola »

When we started Bruce Keith Real Estate Coaching & Seminars, we had been going strong in real estate sales for 10 years as a husband and wife team. We could have kept going. At that point, we had been consistently coming in with top sales for our brokerage, having closed more than 1000 deals. But we realized our passion was not in our own success. It was in helping others get to where we were. We noticed many real estate agents starting out and struggling. Once they graduate from their qualifying program, they approach the world of real estate with little to no sales skills. But without a strong toolset from which to begin your real estate sales journey, you might as well be shooting arrows in the dark. It's a talent that needs development, just like any other career. But many still don't realize that. Bruce first entered the coaching world by working for three of the top real estate coaching companies on the market. He spent 11 years doing that before starting his own, boutique-style, real estate business coaching company. We wanted to change the experience for struggling agents, or for the ones that had stagnated, and weren't sure why. We saw a lot of potential in these salespeople. It just wasn't being utilized or perfected by brokerages, or anyone else. And that's expected; this industry is commission-based. Most of the time, it's the survival of the fittest out there. The fastest ones, the strongest ones and the naturally talented ones make it. But the ones who just need a little nudge often don't. So how do you accelerate a person from '0 to 60 mph' in real estate? That's what we figured out. How? By having been there, and done that ourselves. Sure, we had some background in sales and marketing working at IBM. But no one taught us what the plight would be like, transitioning from predictable salaries to commission-only incomes. No one taught us how to start a business from scratch. And certainly, no one taught us how to work efficiently – and successfully – as a husband and wife team in real estate (trust us, that took some work!). We learned all of the above the hard way – by our 'feet on the street,' and through the 'daily grind.' We woke up every day mastering the art of getting over our failures fast. This was so we could make the climb to the next level without wasting time. We discovered how to stay strong, and how to keep learning, so we wouldn't get 'stale.' We took control of our future – we didn't let circumstances decide it. Today, we teach those hard-learned lessons in our real estate training programs. The formula that took us years to perfect is now easily available through information products, seminars or personal, one-on-one real estate coaching services. Our real estate coaching company has seen a lot of success in others, through what we've managed to put together as teachable material. Our clients have doubled, tripled or quadrupled their sales revenue. They constantly tell us how much our training has helped them, and how different it is than what's out there. And to be honest, that makes us really happy, and proud. We wanted our real estate coaching company to be different at the outset. We just didn't believe that fancy marketing was the secret sauce to real estate sales. Nor were cookie-cutter manuals other coaches in the industry were (and are) using. You can't 'factory produce' promised results. Sure, you can use those methods – but they don't explain everything. Like, why do they work for some and not others? Many have encountered that mystery, we believe. Our training is different because it's not theory, and it's not about marketing investments. It's about the real, honest-to-goodness truth of waking up every day, and doing your 'homework.' We teach you what to do right now to make sales in real estate. We also keep you accountable to your goals in a practical way. Bruce will actually get you to call him at 6 a.m. if that's your goal. And he's not joking about making sure you do. Going above-and-beyond that, we also pay attention to what makes our clients unique. They each have strengths and situations they can utilize to their advantage. Situationally, farming a 2,000 home subdivision is not quite like selling commercial office space, or cottages near a ski resort. Personality-wise, our clients may not all be good at an approach like cold calling. But they do have something they can use to 'connect' with their leads. We help real estate agents find their 'nugget.' And in doing so, we stay away from trying to change their weaknesses. That can be the wrong place to waste your time, if you're going to waste it in the first place. We love doing what we do, because we get to watch, on a daily basis, how much real estate sales training makes a difference in people's lives. Watching the growth and maturation of a person in real estate sales is truly rewarding to us. We hope to continue going strong, with our amazing clients, for as many years as we can!

Holland America Line Explores Three Mexican Riviera Ports on Extended Cruise Season in 2019 PRESS RELEASE PR Newswire Sep. 20, 2018, 01:56 PM Three ships to feature 22 seven- and 11-day cruises through 2019 SEATTLE, Sept. 20, 2018 /CNW/ -- From mariachi to margaritas, the vibrant spirit of Mexico comes alive aboard three Holland America Line ships during the 2019 Mexico cruise season. From January through December 2019, Eurodam, Nieuw Amsterdam and Oosterdam will sail a series of seven- and 11-day cruises roundtrip from San Diego, California, that include calls at Mazatlán, Cabo San Lucas and Puerto Vallarta, Mexico. Boasting sun-kissed skies, golden beaches and azure seas, the Mexican Riviera is an ideal getaway for travelers looking to visit a tropical destination that's rich in culture, color and cuisine. On board, Explorations Central (EXC) programming brings the country to life through local entertainment, regional dishes, destination presentations and more. "Mexico cruises are among our most popular because they are perfect for a traveler who wants both a sun and beach filled itinerary along with a culturally rich destination wrapped into one vacation," said Orlando Ashford, president of Holland America Line. "By featuring up to three Mexican ports of call on one itinerary, our cruises offer a deeply comprehensive and immersive opportunity to become connected to the people and traditions of Mexico." Mexican Riviera Sailings Roundtrip from San Diego All Mexican Riviera cruises will call at Cabo San Lucas, Mazatlán and Puerto Vallarta, giving guests a great feel for the country and all that it offers. Three sea days provide time to relax and explore the abundance of amenities on board. Guests can expand their cruise to include more of the West Coast aboard Eurodam sailing from Vancouver, British Columbia, Canada, Oct. 15, on a unique 11-day itinerary down to San Diego that also calls at Santa Barbara, California. For those with less time, this cruise can be taken as a seven-day itinerary departing San DiegoOct. 19. Eurodam offers eight additional seven-day cruises through 2019, and its sister ship, Nieuw Amsterdam, will also explore the region on six sailings starting March 17. Oosterdam will sail into the region Oct. 6 on its first of eight seven-day sailing, including two festive Mexican Riviera Holiday voyages departing Dec. 21 and Dec 28. Popular Ports Highlight the Itineraries During the sail into San Diego, guests are treated to scenic cruising past Coronado Island, Cabrillo National Monument and Point Loma. The ship conveniently docks near downtown, so guests can walk on Coronado Island's sandy beach, take in the Old Town or have a wild time at the famous San Diego Zoo, time permitting. At the tip of the Baja Peninsula, Cabo San Lucas is one of Mexico's most recognizable ports of call thanks to its unique rock arch formation. Whether lounging on a powdery beach, exploring on a four-wheel-drive adventure or taking to the sea in search of a gray whale, guests will find Cabo offers something for every type of traveler. Mazatlán, known as the "Pearl of the Pacific," is where the Sea of Cortez and the Pacific Ocean meet, creating an incredible sportfishing environment. It's also a haven for watersports, historic sites and nature. Locals treat visitors like amigos, making it a coastal city with plenty of allure. Puerto Vallarta has it all — from gorgeous beaches and activities galore to chic shops and stylish restaurants. The quaint Old Town greets visitors with an enticing mix of red-tiled buildings, cobbled streets and bustling open markets, while eco-adventures show off Mexico's flora and fauna in this paradise on earth. Explorations Central Brings Mexican Culture on Board Throughout the voyages, EXC programming brings Mexico's local traditions, culinary tastes and cultural experiences to life onboard. Through EXC Encounters, a mariachi band in traditional attire will perform onboard, while a barbecue on Lido Deck features authentic Mexican dishes. Guests who want to learn more about the country can attend an EXC Talk by an EXC Guide or head to America's Test Kitchen for a cooking demonstration, cooking class or themed mixology class featuring local fare. The Dining Room and Lido Market also will showcase the flavors of Mexico, while bars will offer a variety of Mexican liquors and ingredients. The ship's ambiance will be enhanced by Mexican floral varieties, and guests will be welcomed in their staterooms with Mexican towel creations. The EXC Team will provide further customized guidance for the Mexican calls, and the in-stateroom EXC Channel will feature a selection of curated films, documentaries and TV shows exploring destinations on the ship's itinerary. Holland America Line's Explorations Central recently received top honors for outstanding Overall Cruise Education Program from a leading travel industry publication. For more information, contact a travel professional, call 1-877-SAIL-HAL (1-877-724-5425) or visit hollandamerica.com. Editor's note: Photos are available here: https://www.cruiseimagelibrary.com/c/t13qupij. Find Holland America Line on Twitter, Facebook and the Holland America Blog. Access all social media outlets on the home page at hollandamerica.com. Holland America Line's fleet of 14 ships offers more than 500 cruises to more than 400 ports in 98 countries, territories or dependencies around the world. From shorter getaways to 113-day itineraries, the company's cruises visit all seven continents with highlights including Antarctica explorations, South America circumnavigations and exotic Australia/New Zealand and Asia voyages; four annual Grand Voyages; and popular sailings to the Caribbean, Alaska, Mexico, Canada/New England, Europe and the Panama Canal. The line welcomed Koningsdam in 2016 and has a second Pinnacle Class ship,Nieuw Statendam, to be delivered in November 2018. A third Pinnacle Class ship, due for delivery in 2021, recently was announced. The company is undergoing $300 million in brand enhancements to secure its position as the leader in premium cruising. Fleetwide, the ships feature innovative initiatives and a diverse range of enriching experiences focused on destination immersion and personalized travel. Guests can expand their knowledge through an exclusive partnership with O, The Oprah Magazine; during an America's Test Kitchen show; at Explorations Café, powered by The New York Times; and by taking a Digital Workshop powered by Windows. Outstanding entertainment fills each evening at venues including Lincoln Center Stage, Billboard Onboard and B.B. King's Blues Club. The dining experience can be savored at a variety of restaurants with menus that feature selections from Holland America Line's esteemed Culinary Council that comprises world-famous chefs who design dishes exclusively for our guests. CONTACT:Sally Andrews EMAIL:pr@hollandamerica.com View original content to download multimedia:https://www.prnewswire.com/news-releases/holland-america-line-explores-three-mexican-riviera-ports-on-extended-cruise-season-in-2019-300716329.html SOURCE Holland America Line Stock News /

UNDERSTANDING SCREENWRITING: Lots of Women and a Few Guys Tom Stempel analyzes A Simple Favor; The Old Man and the Gun; Juliet, Naked; The Book Shop; Operation Finale; Colette; All About Nina; The Sisters Brothers; and the book, When Women Wrote Hollywood. Tom Stempel My apologies to Anna and Henry. A Simple Favor (2018. Screenplay by Jessica Sharzer, based on the novel by Darcey Bell. 117 minutes) Anna Kendrick and Blake Lively in 'A Simple Favor' Paul Feig, the director of this film, is primarily known as a director of comedies. He's directed television comedies, and he has had spectacular box-office success with his recent theatrical features, including Bridesmaids (2011), The Heat (2013), and Spy (2015). He wanted to try something different, and when Sharzer's screenplay, which had been turned down by a lot of people, came to him, he could see it as a "Hitchcockian" picture, which he wanted to do. Thank God it is not a Hitchcockian picture. It's much more interesting than something in the traditional Hitchcock line. I have always thought Charles Bennett's Fat English Friend, as he's known in these parts, was much more interested in scenes than character. Some people objected to that when I mentioned it in this column back in its House Next Door days. You can read the wrap-up of that discussion here. What Feig has in Sharzer's script is as much a character study as a thriller, although it is not without its thriller moments. We first meet Stephanie [Anna Kendrick] when she is doing her vlog about cooking for mothers, then learn how she became best friends with Emily [Blake Lively], the mother of a son in Stephanie's son's class. Emily is about as opposite of Stephanie as you can get. She's rich, married to a novelist with writer's block, has a marvelous house, and works in a glamorous job in the city, i.e., New York. No wonder Stephanie is enchanted by her. And perfectly willing to pick up Emily's son from school and have him over for playdates and…well, one day Emily goes missing. That brings an element of mystery into the story, but Emily is so interesting a character we are sorry to not have her around. Even more so when she turns up dead, drowned in a lake in Michigan. Except then Emily's son begins to think he sees her… But we've seen the body… Stephanie is so involved with Emily that she begins to play detective on her own. It turns out she is really good at it, and is great at lying to people to get information from them. Just as Emily and Stephanie are great characters, so are the people Stephanie meets on her quest. I suspect one reason people kept turning down the script is that the focus is on the women, all of whom are interesting characters. The men are very such secondary characters, which I think makes the film more interesting. Want Complex Female Characters? Put Them in Action So the film provides a lot of great work for the actresses involved. In writing here about Pitch Perfect I said of Anna Kendrick, "I hate to say it, but on the basis of this film, she may end up a great character actress rather than a star. Unless somebody out there, hint, hint, writes a really great star part for her." In writing about Pitch Perfect 2, I qualified that by saying, "Beca is a little looser than before, and I may have to take back my comment in the review of the first film that Anna Kendrick can't carry a picture as a star." On the basis of A Simple Favor, I offer my sincere apologies to Kendrick, who plays Stephanie. Boy, does she carry this picture. Blake Lively is just as good as Emily in what is maybe her best performance yet. And Jean Smart and Linda Cardellini are not wasted in supporting roles. The men are definitely secondary, but I also have to add another apology to Henry Golding. I did not think he was all that impressive as an actor in Crazy Rich Asians, but here he shows some actual acting chops. So you have a good script, a good cast, and a director smart enough to stay out of their way. Good career choice, Paul. Twinkle, Twinkle, Major Stars The Old Man and the Gun (2018. Screenplay by David Lowery , based on the New Yorker article by David Grann. 93 minutes) Forrest Tucker [Robert Redford] (no relation to the action movie star of the 40s and 50s) is an old man who very politely robs a bank. And not for the first time. What makes this time interesting is that while being chased by the police, who are some distance behind him, he stops to help Jewel [Sissy Spacek], a woman who is probably not quite as old as he is. Her truck is on the side of the road with the hood up. Forrest stops, gets out, comes over and looks at the engine. And the cops drive right by. Because Forrest is so polite, Jewel goes to a coffee shop with him and they chat. He says he's a salesman, the later tells her the truth, but in a way she won't believe. He is charming. She is just as charming. If you have seen the trailer for this film, you have seen most of this scene, which takes up a greater part of the trailer, and rightly so. Forrest is played by Robert Redford and Jewel by Sissy Spacek. They bring their full star-power to this scene and to the rest of the movie. They twinkle big time, as only major stars can. Lowery has written a great scene for them and they act the begeezus out. Lowery also directed, not very well (he does not have the best sense of where to put the camera), but the writing and the stars carry it, and the movie. We get more bank robberies, and a glum cop chasing Forrest, and at least one more great scene with just Forrest and Jewel. We also get another great scene when the cop goes to see who he thinks is Forrest's wife, but turns out to be his daughter. You will recognize the actress on her day off from her regular starring role and she, like Redford and Spacek, acts the begeezus out of her scene. Not as much twinkle, but incredible acting skill. Juliet, Naked (2018. Screenplay by Evgenia Peretz and Jim Taylor & Tamara Jenkins, based on the novel by Nick Hornby. 105 minutes) You can tell this movie is based on a Nick Hornby novel immediately when we meet Duncan, who is obsessed with an American musician Tucker Crowe, who has gone into hiding. What makes the film interesting is that Duncan is not the main character. His wife Annie is. When Tucker sends Duncan a pre-release version of his greatest hit, Annie writes a review of it on Duncan's website, which leads to a relationship developing between Tucker and Annie. This leads to great scenes. (The actors, Rose Byrne as Annie, Ethan Hawke as Tucker, and Chris O'Dowd as Duncan, are wonderful.) One great scene is Tucker in the hospital with his assorted children, girlfriends, et al piling up in his room. It's the best multi-character scene since the finale in Tangerine (2015; see my review here for more detail about writing multi-character scenes). The Bookshop (2018. Screenplay by Isabel Coixet, based on the novel by Penelope Fitzgerald. 113 minutes) Florence, a young widow in a small sea-side village in Post World War II England, decides to open a book store. Unfortunately, the building she picks is coveted by Violet Gamart. Gamart is the queen bee of the village and does everything she can to stop Florence. The movie is charming (with great performances by Emily Mortimer as Florence and Bill Nighy as Mr. Brundish, the town recluse), but there is no dramatic tension, just one downhill slog. SCENE FIX: Tips for Elevating Tension, Characters and Conflict Operation Finale (2018. Written by Matthew Orton. 122 minutes) Since 1961, when infamous Nazi Adolph Eichmann was captured by Mossad, the Israeli intelligence force, there have been a number of films about Eichmann and his capture, which is why when I was watching this I kept thinking of the 2004 film The Alamo. There had been a lot of films about the Alamo, so why make another one? The only answer for the 2004 film was that so Billy Bob Thornton could play Davy Crockett. He was great, but the rest of the movie was stuff we'd seen before. In Operation Finale, the newest element is that Eichmann is played by Mahatma Gandhi himself, Ben Kingsley. Well, he's great in the part, but the rest of the movie is, as with The Alamo stuff we've seen before. So the lesson is, if you are going to do something that's been done before, you had better bring a really fresh approach to the material. Sometimes, sadly, Billy Bob Thornton and Ben Kingsley are just not enough. Colette (2018. Screenplay by Richard Glatzer & Wash Westmoreland & Rebecca D. Lenkiewicz, story by Richard Glatzer. 111 minutes) This is about the early life of the great French writer Colette, but it spends too much time on the early years of her marriage to Willy, a literary entrepreneur who publishes her books under his name, both for commercial and egotistical reasons. We know she's going to demand they be published under her name, but the movie takes forever to get to that. When she finally gets a publisher to print one with her byline, all we get is a shot of the book in a store window and then titles that describe the rest of her career. We don't have to see that, but since the film has been building to her name appearing on a book, at least we ought to see her enjoying it. Especially since they have Keira Knightly in her best performance surprisingly cast as Colette. All About Nina (2018. Written by Eva Vives. 97 minutes) A similar problem here. Nina is a foul-mouthed comedian who moves from New York partly to get away from her abusive boyfriend and mostly to try to get a television gig. She falls in love with a great guy, Rafe, but she has no idea how to deal with a nice guy. Doing a gig, she has an emotional breakdown and spews out a lot of details about what really happened to her as a kid. So she blows the TV gig? No, and there is talk about how she, the TV producer, her agent, and a publicity person may be able to handle it. But we don't get enough of that, which would be fresher than the love story with Rafe. If Vives, who also directed, was limited to 97 minutes, she could have condensed the love story and developed the end. Or if she could get more time, she could have added on to the end. What she has here at least gives Mary Elizabeth Winstead, whom longtime readers of this column know I have been mad about for years, a terrific opportunity to stretch her already impressive talents as Nina. The Sisters Brothers (2018. Screenplay by Jacques Audiard and Thomas Bidegain, based on the novel by Patrick Dewitt. 121 minutes) I love westerns. I love shaggy dog stories. So I should love this shaggy dog western. But I don't. It may be just a bit too shaggy, or maybe not shaggy enough. We follow the Sisters Brothers, Eli (John C. Reilly) and Charlie (Joaquin Phoenix), two hired assassins in the west as they try to track down Hermann Kermit Warm, a scientist who has discovered a new way to pan for gold. The brothers' boss, the Commodore, wants him killed, although we don't know why. The Commodore also has a tracker, John Morris, trying to find Warm for the brothers. They all meet up, get to be friends, and some of them die. There just is not that much happening to hold our interest, and the dialogue can't hold our interest much either. Listen to William Goldman's dialogue in Butch Cassidy and the Sundance Kid (1969) for how it ought to be done. Q & A with Director Caroline Case, 'Nobody Knows Anything (except William Goldman)' Two other things, one bad, one good. The bad: as in Solo: A Star Wars Story (2018), the lighting is so dark we can't see the faces. I suspect Joaquin Phoenix is giving a better performance than we can see. The good: unlike Colette and All About Nina, the ending, especially the last meeting with the Commodore, is nicely developed. Youngsters Writing About Oldsters When Women Wrote Hollywood (2018. Book edited by Rosanne Welch. McFarland [McFarlandBooks.com]. 221 pages) Rosanne Welch is a television writer who also teaches screenwriting at a variety of places. One of her gigs is handling the Los Angeles residency for screenwriting courses offered at Stephens College in Missouri. The students come out to L.A. a couple of times a year, where they get lectures from people connected to the business. One assignment that Welch has her students do is research papers on screenwriters of the past. This book is a collection of those papers, 23 by her students and one by Welch. Stephens used to be an all-women's college, but it now takes male students. The preponderance of its students are female, so all of the essays, including two by male students, are about women screenwriters in the early days of Hollywood. Some writers, like Anita Loos, you have probably heard of. Many of them you probably have not. I was particularly taken by Amelia Phillips's piece on Jeanie Macpherson. I wrote briefly about Macpherson in my book FrameWork: A History of Screenwriting in the American Film (1988), but one reviewer gave me a hard time for not mentioning that she was Cecil B. De Mille's mistress. He seemed to think that disqualified her as a writer. Phillips starts out in the first paragraph by noting that Macpherson was only one of De Mille's three long-time mistresses and has credits on a lot more than just De Mille's films. Several of the pieces, such as the ones on Zoe Atkins and Bella Spewack, note that they worked in both the theatre and film, which was a lot more common than is generally assumed about the early days of movies. Welch takes her students to the Margaret Herrick Library of the Motion Picture Academy and some get into the archives in depth. Others, such as the people writing on Anita Loos and Dorothy Parker, depend mostly on memoirs and biographies. Then there is Pamela Scott, who found very little material on Sarah Y. Mason, the wife and co-writer of Victor Heerman, but was able to follow her connections with other people to give a nice little view of Mason's career. Like virtually every other book that is a collection of essays by different writers, the quality varies a lot, but there is enough good stuff to make it worth your while. More articles by Tom Stempel What are you doing that could be thwarting your writing career? Learn the Habits of Highly Successful Screenwriters! The Old Man and the GunAll About NinaA Simple FavorOperation FinaleColetteThe Book ShopJuliet NakedWhen Women Wrote HollywoodThe Sisters Brothers Tom Stempel taught film history and screenwriting from 1971 to 2011 at Los Angeles City College, and has contributed to Slant Magazine and Creative Screenwriting. He has also written six books on film, five of them about screen and television writing. You can learn more about his books here. His latest book is Understanding Screenwriting: Learning from Good, Not-Quite-So- Good, and Bad Screenplays. The book evolved into his column "Understanding Screenwriting." In the column he reviews movies and television from the standpoint of screenwriting. He will be looking at new movies, old movies, and television movies and shows, as well as writing occasional other items, such as appreciations of screenwriters who have died, plays based on films, books on screenwriting and screenwriters, and assorted other sundries.

A bi-monthly interview series helmed by Kevin Sharp (Bookmarks, The First Line, After Dakota) that highlights comic book creators of all experience levels, providing insight into their creative process, working space, passion for the sequential art medium, and more. 'Reign of the Supermen' Premiere: Jerry O'Connell's Fallen Supeman Returns? At the Paley Center for Media (Los Angeles), Fanbase Press' Bryant Dillon talks with actor Jerry O'Connell (The Death of Superman, Stand by Me) about his work on Reign of the Supermen, what Superman inspires in others, and more. At the Paley Center for Media (Los Angeles), Fanbase Press' Bryant Dillon talks with actor Jason O'Mara (The Death of Superman, Agents of S.H.I.E.L.D.) about his work on Reign of the Supermen, Batman's feelings regarding the death of the Man of Steel, and more. The following is an interview with publisher, editor, molecular biologist, and author Athena Andreadis, Ph.D. In this interview, Fanbase Press Editor-in-Chief Barbra Dillon chats with Andreadis about the inspirations behind her impressive career, her work with publishing company Candlemark & Gleam, her own writing, and more! The following is an interview with Desmond Walsh on the release of the comic book series, Jungle City. In this interview, Fanbase Press Editor-in-Chief Barbra Dillon chats with Walsh about the inspiration behind the series, his creative process in bringing the story to life, what readers can anticipate from the story, and more! The following is an interview with award-winning comic book artist and writer Sean Lam on the graphic novel series, Red Dot. In this interview, Fanbase Press Editor-in-Chief Barbra Dillon chats with Lam about the inspiration behind the series, his creative process in working with kingB, what readers can anticipate from the story, and more! The following is an interview with Arwen Elys Dayton on her new science fiction novel, Stronger, Faster, and More Beautiful. In this interview, Fanbase Press Editor-in-Chief Barbra Dillon chats with Dayton about the inspiration behind the novel, her creative process, what readers can anticipate from the six-part story, and more! The following is an interview with Ross Joseph Gardner on the comic book series, Half-Life: A Place in the West. In this interview, Fanbase Press Editor-in-Chief Barbra Dillon chats with the Gardner about the inspiration behind the series, his creative process in adapting the story from its original format, what readers can anticipate from the story, and more! The following is an interview with Matt and Brittany Loisel on the comic book series, Murder. In this interview, Fanbase Press Editor-in-Chief Barbra Dillon chats with the Loisels about the inspiration behind the series, their shared creative process, what readers can anticipate from the story, and more! The following is an interview with Dirk Wood, editor of Full Bleed magazine from IDW Publishing. In this interview, Fanbase Press Editor-in-Chief Barbra Dillon chats with Wood about the launch of the Kickstarter campaign for Full Bleed: Volume 3 - Heavy Rotation, what readers can anticipate from the latest release, the cool backer rewards in store, and more! The following is an interview with author Matt Maxwell on his new novel, Queen of No Tomorrows. In this interview, Fanbase Press Editor-in-Chief Barbra Dillon chats with Maxwell about the inspiration behind the book, his creative process, what he hope that readers will take away from the story, and more!

Before now, you could only find wild cobia fillets on discriminating fine-dining menus. But with World Port Seafood you can find it on your doorstep, ready for dinner. It's a lean fillet with mild-to-moderate savory flavor that's caught in the certified-sustainable sea waters off the coast of Indonesia.

Having served as an elementary school teacher for four years, Dave H. Cho found that teachers in public school systems are not equipped with the best content to educate students or adequate tools and resources to evaluate them. Besides, they are burdened with added responsibilities which limit them from fulfilling even their basic task. Realizing this problem, Cho decided to quit his job and initiate an educational service that led to the birth of Classting. The EdTech startup develops a learning management system (LMS), Classting Learning that can enhance the learning management and experience for both students and teachers. On top of that, Classting builds an educational, social networking site (SNS) to facilitate communication among teachers, students, and parents. To empower teachers with top-notch tools and content, Classting partners with 25 of the country's leading content providers. Through Classting Learning, teachers can easily find high-quality content online and get students to learn at their own pace. Once students are done with learning, their performance statistics appear on an analytics dashboard that teachers can review. Based on the statistics, teachers can then provide feedback to students individually via the social networking platform while also recommending suitable content according to each student's caliber. By reviewing the active learning data generated within the service using AI, Classting provides the most accurate and satisfying adaptive learning. "With the expansion of our service, students and parents can easily and objectively check the current level of learning so that they can plan more effective learning methods in the future," says Dave H. Cho, founder & CEO at Classting. On the technology front, Classting is at the leading-edge bringing advanced technologies such as AI, machine learning, and IoT into education via its solution. At the core of its LMS is machine learning that analyzes students' learning curve and selects content that will help them improve their performance on their own. With the recent upgrade of the analysis report function, students and parents can check their personal analysis data in real time on mobile and PC, so that they can further work on their drawbacks at home. By applying 'Deep Learning' technology, students are able to do more efficient personalized learning based on each vulnerable part. Moreover, Classting fulfills both functions of LMS and SNS. "We have created an environment where big data related to education can accumulate and modify technology to enable individual learning," Cho notes. However, Classting is different from other SNS, in that unlike other SNSs, which are open to many unspecified students, Classting shares contents, distinctly related to learning, with closed service created for school use. At present, Classting has more than 10,000 contents developed by more than 20 major education companies in Korea and abroad. With the insights gained from Korea, the team at Classting plans to expand to other public schools in Asia, such as Singapore, Taiwan, and Japan. Having successfully conducted pilots in Taiwan and Japan, the team is currently in negotiation with a distribution partner in Singapore. "Top investors including Softbank and Samsung today believe in Classting's vision to improve the education environment through technology," extols Cho. The company has taken quite a big leap, from its inception in 2012 to solidify its brand recognition with Classting Learning in 2017, and resolves to continue its support and contribution to public education. Classting's near-term goals include focusing its capabilities on platform development and intensifying content recommendation and curation services by cooperating with carefully selected contents.

Cell & Microbiology Agriculture - January 23, 2023 Microbes could be used by farmers as natural fertilizer for poor soil A study published in The ISME Journal identified 522 genomes of archaea and bacteria associated with the roots and soil of two plant species native to the Brazilian montane savanna ecoregion known as campos rupestres ("rocky meadows"). Hundreds of microorganisms hitherto unknown to science were identified, showing that the ecoregion is a biodiversity hotspot and that many new organisms have yet to be described and classified in Brazil.

Chachahuantla is a growing Nahua town right on the border with Hidalgo. There blouses are sold to a number of other villages in the vicinity. In the first picture we have the "new" version of the quechquemitl, in my mind it is nicer and will be easier to sell to the Mexican consumers since it can be worn with just about any type of clothing. Translating the village embroidery into a commercial product is a continuing problem, compounded by the diversity and competition from other indigenous groups and villages. Above there are three examples of the blouses from the town. The last blouse is embroidered in the old style. In the future, I will be returning to this area to do an in-depth study of the techniques and styles with the help of this collective. Right now it is difficult to tell which village is which when it comes to the style of blouse. It may turnout be a personal choice based on economic factors, which blouse a person buys. That assumes they are not the embroiderer. The Saturday market in Huauchinango is a great place to visit and enjoy the diversity of textiles from the region.

Current Honda special offers from D. M. Keith - Business, New & Approved offers and our latest Motability offers. Interested in all the latest Honda deals and special offers available from D. M. Keith? You've come to the right place! At D. M. Keith Grimsby, we're committed to delivering value for our customers across our full range of services. From New Honda to Approved Used, Motability and Corporate, to Service and Parts offers. We'll get you in an award-winning Honda at the lowest possible price and make sure you continue to save until you hopefully return to buy another Honda from us. Service special offers: lowering the cost of keeping your Honda in perfect condition. The HR-V is a coupé-styled SUV with all the smart looks of its bigger brother. It's amazing how many innovative ideas have been packed into one stylish and spacious car. The new CR-V shares your spirit for adventure and gives you the freedom and flexibility to explore. Rethought and redesigned inside and out, the Honda Civic is built to be driven, but it can be much more than that.

Our recently developed QQR-type integral screening is introduced in our Cholesky-decomposed pseudo-densities Møller-Plesset perturbation theory of second order (CDD-MP2) method. We use the resolution-of-the-identity (RI) approximation in combination with efficient integral transformations employing sparse matrix multiplications. The RI-CDD-MP2 method shows an asymptotic cubic scaling behavior with system size and a small prefactor that results in an early crossover to conventional methods for both small and large basis sets. We also explore the use of local fitting approximations which allow to further reduce the scaling behavior for very large systems. The reliability of our method is demonstrated on test sets for interaction and reaction energies of medium sized systems and on a diverse selection from our own benchmark set for total energies of larger systems. Timings on DNA systems show that fast calculations for systems with more than 500 atoms are feasible using a single processor core. Parallelization extends the range of accessible system sizes on one computing node with multiple cores to more than 1000 atoms in a double-zeta basis and more than 500 atoms in a triple-zeta basis.

Duncan Craig LLP recognizes agriculture and its related value-added sector as leading industries in our province. Agribusinesses and farmers can face a wide variety of legal issues, which are often complex because of the impact of several areas of law. The agribusiness group or lawyers at Duncan Craig LLP ensures that we remain current with the ever-increasing body of legislation, regulations and case law affecting your farm or agribusiness. We are committed to providing a comprehensive range of legal services to the agricultural community, and to delivering these services in a timely and cost-effective manner.

Summary: How to login using your old email, and update your email address. On the home page, click "Login/Subscribe". This brings up the login form. Log onto Bivouac using your old email address as the login code. If you are successful, you will be returned to the home page, and you'll see your old email address on the top of the screen, in green. If you can't remember your old email address, see instructions for Forgot my password. Click on your email address. This brings up "member Settings" page. Click "Update Your Member Record". This brings up an update form. Fill in the new address, and click "Update". This will return you to your "Member Settings" page. Note that at the bottom of your member settings page there is a heading called "Email Validation", and under that it tells you your address is "unconfirmed". Click the link titled "Send Yourself a Validation Message". This sends you a validation email. Go into your email "In Box" and look for a message with the title "Bivouac Self Email Validation". Go into the message, and click on the link in the message. This should set the status of your new email address to "Good". Everything is now ready to go. If you went to your Member settings Page again, you will see that you are confirmed.

Save Our Sacheen submits the attached Proposed Changes with Comments for the consideration of the Planning Commission at its upcoming meeting on June 14, 2007. These proposed changes are requested to protect Pend Oreille County's valuable water resources, particularly its smaller lakes such as Sacheen Lake, from the dangers and harms of overcrowding the lake through "keyholing", overly large RV parks and other over development. Save Our Sacheen members believe that Sacheen Lake must be protected from over development that might result if the proposed changes are not adopted. Save Our Sacheen thanks the Planning Department, the Planning Consultant and the Planning Commission for their consideration of these Proposed Changes and urges the Planning Commission to adopt these proposed changes. For your information Save Our Sacheen is a 2006 Washington Non-Profit Corporation whose primary purposes is to save Sacheen Lake from the various problems that confront the lake and the landowners that surround the lake. Its membership is approximately 150 members. I am the duly elected President of the organization. Save Our Sacheen submits the attached Sacheen Lake Subarea Moratorium for the consideration of the Planning Department, Planning Consultant, Planning Commission and County Commissioners and requests that the County enact this Moratorium. Save Our Sacheen has also submitted Proposed Changes to Development Regulations with appropriate cover letter. Save Our Sacheen still requests that these proposed changes be adopted in addition to the County Commissioners enacting the Sacheen Lake Subarea Moratorium. The attached Moratorium is necessary to grant additional time to all residents in the Sacheen Subarea to consider a plan tailored to the needs for Sacheen Lake. Now that Sacheen Lake residents have had time to consider and evaluate the proposed development regulations, several concerns have arisen. One of these concerns is a potentially large development of old Sacheen Lake Resort which could drastically change the character of the lake. In the past there has been a suggestion that the Sacheen Lake Subarea Plan should be a Limited Area of More Intensive Development (LAMIRD). Sacheen Area residents are largely (near unanimously) opposed to more intense development. The particular problems confronting Sacheen Lake are best addressed by a Subarea Plan (and implementing development regulations for Sacheen Lake) rather than generalized plans and regulations. Subdivisions, Water Access Lots (keyholing), Recreational Vehicle Parks, Master Planned Resorts, Planned Unit Developments, Marinas. The moratorium shall be effective for one year from date of enactment if a work plan is developed for related studies which may be extended further until completion of the Subarea Plan and implementing development regulations. 4B Boat Berth: A boat berth is a space on a dock or pier not less than 25 feet to provide parking of a single boat. 11B Keyholing: The practice of providing access through a water access lot water body for many users or lots located elsewhere. 35. AWater Access Lot@: A water front lot upon a lake which is used to provide access to the lake for to limited specific group of people rather than the general public. Comments: These additional definitions pertain to the following proposed changes including limitations on the practice of keyholing through water access lots and limitations upon docks and larger docks such as marinas which would overcrowd the surface waters of Pend Oreille County. Comment: The current development regulation needs some teeth. The County should specifically state that the Water Access Management Plan and the associated development application can be denied. The grounds for denial of approval of the Plan should also be stated. If over use of a water resource is permitted, then the valuable water resource will be lost for the current uses of the water resources and the water resource will be lost for all. If the boating traffic on the lakes and rivers of Pend O=reille County become like boat traffic on Coeur d=Alene Lake or Bayview on Pend O=reille Lake, then boating will not become a lesser and lesser desired use. Comment: The Sacheen Lake Area is already overdeveloped and cannot support more residences on its shoreline. Limited Areas of More Intensive Rural Development allow for infill of the LAMIRD area which would only increase the overcrowding that already exists. Infill is not needed or desired at Sacheen Lake and would in fact be harmful to its environment by adding more people than the lake can support. A. Water Access Lots. All other uses other than water access. Comment: If a lot is designated as a water access lot, that use should not be mixed with other residential uses or other uses. Such other uses would be incompatible with the water access use. Marinas are not allowed on lakes smaller than 350 acres in size. C. Docks on lakes over 700 acres: 100 feet. The number of docks for each lot and each water access lot is limited to one dock. Comment: If the length of docks are not limited, then the owners of the adjacent land tend to extend the length of docks leading to decreased area in the lakes for other boat users. Limitations on length are reasonable and for a valid purpose. and impacts to the environment including erosion. with a density of 1 dwelling per lot. (not 3rd tier lots) with a density of one dwelling per lot. Comment: Water access lots should not be approved if they would lead to overcrowding and damage to the lake environment. A. Lake 350 acres or less: maximum of 20. B. Lake 351 to 700 acres; maximum of 40. Comment: If the number of RV units is not limited, then overcrowding of the water body will occur. Recreational Vehicles are in ever growing popularity. To protect the water body from overuse, limitations on the number of vehicles is required. 1. 5 to 25 1 to 4 Back Lots: Recreational area with water frontage equal to the average water front lot width for individual lots within the subdivision in feet of 75 feet and containing no less than 7500 square feet. 2. 26-50 5 - 25 Back Lots: Recreational area with water frontage equal to twice the average water frontage lot width for individual lots within subdivision in feet of 150 feet and containing no less than 15,000 square feet. 3. More than 50 25 Back Lots: Recreational area with water frontage equal to twice the average water frontage width at the ordinary high water line of individual lots within the subdivision 225 feet plus two additional lineal feet for every back lot over 51 25. Minimum 15,000 square feet. Comment: To protect a water body from overuse, It is necessary to limit water access lots for secondary and tertiary development. These revised regulations prevent excessive overcrowding as opposed to the current draft.

Induction of apoptosis by pistachio (Pistacia vera L.) hull extract and its molecular mechanisms of action in human hepatoma cell line HepG2. Several important Pistacia species such as P. vera have been traditionally used for treating a wide range of diseases (for instance, liver-related disorders). There is a relative lack of research into pharmacological aspects of pistachio hull. Hence, this study was aimed at investigating whether pistachio rosy hull (PRH) extract exerts apoptotic impacts on HepG2 liver cancer cell line. In order to evaluate cell viability and apoptosis in response to treatment with the extract, MTT assay and Annexin-V-fluorescein/propidium iodide (PI) double staining were performed, respectively. Moreover, molecular mechanism of apoptosis induced by the extract was determined using human apoptosis PCR array. Our findings showed that PRH extract treatment reduced cell viability (IC50 ~ 0.3 mg/ml) in a dose-dependent manner. Flow cytometric analysis revealed that the extract significantly induced apoptosis in HepG2 cells. In addition, quantitative PCR array results demonstrated the regulation of a considerable number of apoptosis-related genes belonging to the TNF, BCL2, IAP, TRAF, and caspase families. We observed altered expression of both pro-apoptotic and anti-apoptotic genes associated with the extrinsic and intrinsic apoptosis signaling pathways. These results suggest that the aqueous extract of PRH possesses apoptotic activity through cytotoxic and apoptosis-inducing effects on HepG2 cells.

Many women worry about what to eat while pregnant. It's true that that you are what you eat, your baby deserves only the best. An unborn baby is like a sponge, soaking in the nutrients you consume, or soaking in the 'fillers' you consume if you just eat junk foods. It's vital to watch your diet during pregnancy and to make sure you feed your baby the nutrients that will help your developing fetus grow and thrive. There are certain nutrients that can help a child excel in several areas. As an example, take a look at what the consumption of omega 3 fatty acids can do for a growing fetus. Research in Europe (European Commission study of 2,000 pregnant women who consumed 'oily fish') has shown startling evidence that omega 3's help the child to have better social skills as well as better motor development. There pregnant women consumed salmon, sardines, and tuna, which contained high levels of omega 3 and omega 6 fatty acids. Omega 3 krill oil is safer because it is pure and therefore delivers better results. It's also higher in antioxidants. What you decide to eat while pregnant also affect kids long-term. Test scores of the children of mothers who consumed the most oily fish were very high with motor skills and social abilities. We know that omega 3 alone helps with the development of the brain and eyes during pregnancy. DHA acid, which is found in omega 3 fatty acid, is also found in the membranes of brain cells. It is apparent that when pregnant mothers consume the oily fish, the omega 3 fatty acids and in particular, the DHA passes through to the fetus. Omega 3 fatty acids play a big role in the development of babies who excel with brain development. Aside from how omega 3 fatty acids affect the baby, what to eat while pregnant also affects the mood of the mother. Women who consume the oily fish are less likely to have post-natal depression. Post-natal depression affects over 10 percent of new mothers, and the worse cases hang on for over a year. These women often have to turn to prescription medications to help deal with the depression. Certainly, if eating this oily fish or taking a supplement with omega 3 fatty acid helps to prevent this, it's well worth it. Some oily fish contain higher than normal levels of mercury, which has caused the medical establishment not to recommend consumption of fish during pregnancy to avoid mercury poisoning. Also, there is a risk of eating too much oily fish and "overdosing" on omega 3 fatty acids. So, pregnant women are left wondering what diet to pursue. Since oily fish is a risk for mercury poisoning and it is too easy to eat too much fish thereby giving the fetus too much omega 3 fatty acids, the safe answer is to take an omega 3 supplement. These types of supplements help to pass just the right amount of omega 3 fatty acid to the fetus, and both the mother and the baby will benefit. There are many supplements and fish oils, out on the market. Some are superior to others. Always make sure to get the best brands and the best supplements from the best sources. Krill oil is a safe means of omega 3 fatty acids because krill oil is clean from mercury and other pollutants. The #1 species of krill found in the Antarctic Ocean, the E. Superba krill. Because this species of krill comes from the pristine waters in the Antarctic Ocean, it is federally regulated. You don't have to worry about impurities. Taking omega 3 krill oil literally gives your baby a huge head start in life. Your baby will have much higher brain development, better vision and more. The mother benefits in many ways including less morning sickness, etc.

Q: If-else working, switch not I am making an app that has a grid of images with text and each one opens a different activity. It works fine but just for design purposes I want to replace my if-else statements with switch statements (which I assume I can do) however it doesn't work. Right now my working code to set the label on each image is: if(position == 0) textView.setText(R.string.zero); else if(position == 1) textView.setText(R.string.one); else if(position == 2) textView.setText(R.string.two); else if(position == 3) textView.setText(R.string.three); else if(position == 4) textView.setText(R.string.four); else if(position == 5) textView.setText(R.string.five); ect.... I want to use: switch(position) case 0: textView.setText(R.string.zero); case 1: textView.setText(R.string.one); case 2: textView.setText(R.string.two); case 3: textView.setText(R.string.three); case 4: textView.setText(R.string.four); but when I did that ever label was the last one that I defined (in my example it would be "four"). I also have a similar code for each object to start a different intent with the position variable however that does the opposite and makes every intent equal to the first one. Is my syntax wrong or will this not work for my situation? A: Using a break statement after each case should fix the problem. I would also use a default statement as well after the last case. A: This is the solution. You need to use break to avoid going through each case: switch(position) case 0: textView.setText(R.string.zero); break; case 1: textView.setText(R.string.one); break; case 2: textView.setText(R.string.two); break; case 3: textView.setText(R.string.three); break; case 4: textView.setText(R.string.four); break; I would recommend to read the oracle documentation about the switch statement. A: You need to break; after each statement in a case, otherwise execution flows down (all cases below the one you want will also get called), so you'll always get the last case. switch(position) { case 0: textView.setText(R.string.zero); break; case 1: textView.setText(R.string.one); break; case 2: textView.setText(R.string.two); break; case 3: textView.setText(R.string.three); break; case 4: textView.setText(R.string.four); break; } Here's the official tutorial explaining when to and when not to use break;. A: You need to use break statement after eace case operations. In a switch-case statement if you dont use a break statement then all the cases after that specific one will be executed also case 0: textView.setText(R.string.zero); break; A: Don't forget to put break; after each case: like that: switch(position){ case 0: textView.setText(R.string.zero); break; case 1: textView.setText(R.string.one); break; case 2: textView.setText(R.string.two); break; case 3: textView.setText(R.string.three); break; case 4: textView.setText(R.string.four); break; } A: In the Switch-case statements, you need to put break; after each case. switch(position){ case 0: textView.setText(R.string.zero); break; case 1: textView.setText(R.string.one); break; case 2: textView.setText(R.string.two); break; case 3: textView.setText(R.string.three); break; case 4: textView.setText(R.string.four); break; default: System.out.println("not available"); } Also you need to put default: at last, because when all case are wrong that time perform default: action. In the switch-case statement not forgot about break; and default action. A: You need to break; after each branch: switch (position) { case 0: textView.setText(R.string.zero); break; // <-- here // etc } Legitimate uses of switch when you don't break exist, those are called fall throughs; or because you return or throw.: switch (someNumber) { case 0: return 0; // no need for break here case 1: throw new IllegalArgumentException(); // no need to break here case 2: System.out.println("Oh, I got two!"); // fall through case 3: return 3; default: System.out.println("Meh") // No need to break: last possible branch } return -1; will return 3 even if you enter 2. But otherwise you need to break. A: Each break statement terminates the enclosing switch statement. Control flow continues with the first statement following the switch block. The break statements are necessary because without them, statements in switch blocks fall through: All statements after the matching case label are executed in sequence, regardless of the expression of subsequent case labels, until a break statement is encountered. Switch is faster than if-else statement Bottom line : Default is optional(works like else statement in switch), Break is mandatory. Interesting fact: you won't see any compilation error, even if you forgot to place the break statement. A: The switch needs a break with in each case. But in your case it could be done much simpler by defining an array as shown below. String values = {R.string.zero, R.string.one, R.string.two, ... }; Use this to populate textView : textView.setText(values[position]);

Jan 23, 1985 - Precision Surgical Instruments, Inc. A hand held surgical instrument for use in removing body tissue from a human body. It is particularly useful in arthroscopic surgery of joints such as of the knee or shoulder, but may also be used to remove tissue from the stomach, abdomen, jaw or other portions of the body. An auger-like helical cutter is motor driven at speeds in excess of 1,000 rpm within a stationary outer cylindrical sheath member. The helical cutter is ground to provide a generally concave distal end which gives it a generally fish-tailed appearance and the ability to cut endwise, i.e., to effect an axial boring cut. In addition, the cylindrical sheath member is provided with a pair of longitudinally extending, diametrically opposed tab members at its distal end. Each of the tabs has at least one longitudinally extending edge thereof ground to provide a cutting edge which co-acts with the auger-like cutter blade to sever body tissue insertable between the tabs. This invention relates to a hand held surgical instrument for removing body tissue from a human body and, more particularly, to such a surgical instrument that is particularly useful in arthroscopic surgery. Heretofore it has been known to use coaxially mounted hollow tubes in the performance of arthroscopic knee surgery. Side cutting is effected by providing co-acting side edges of the tubes which diverge as cutting proceeds in the proximal direction. The outer tube is stationary and is provided with a side port which is opened and closed on each cycle by the inner rotating tube. Vacuum is used to remove the severed body tissue which is sequentially admitted through the port in the outer tube and then withdrawn through the inner tube. U.S. Pat. No. 4,203,444 to Bonnell et al and U.S. Pat. No. 4,274,414 to Johnson et al are examples of the previously described prior art. These two patents are deficient in a number of respects. First of all, because the blades are made from tubular members, radial deflection is a critical problem. Bonnell et al acknowledges that even slight deflection, even on the order of one or two thousandths of an inch, of the rotating blade radially relative to the stationary blade can result in jamming of tissues cut from the knee. Secondly, neither of the devices in these two patents are useful in cutting straight ahead, i.e., to give an axial bore cut. Both patents are used for side cutting only. Thirdly, because of the manner in which tissue is introduced into the cutters in these patents, namely, by opening and closing a port in the side of a tube, the speed of cutting is much too slow. In U.S. Pat. No. 4,203,444 to Bonnell rotational speeds are limited to 200 rpm or below. In accordance with the present invention the aforementioned difficulties and shortcomings of the known prior art are effectively overcome in the practice of the present invention. In particular, an auger-like cutter blade is rotated within a stationary cylindrical sheath member which supports the cutter blade throughout its length. Both the auger-like cutter blade and the cylindrical sheath are made from stainless steel and deflection of one relative to the other is not a problem. The cylindrical sheath member is provided with a pair of longitudinally extending, diametrically opposed tabs at its distal end. A cutting edge is provided on a longitudinally dinally extending edge of each of the tabs which co-acts with a helical cutting edge of the auger-like cutter blade to provide side cutting of body tissue insertable between the tabs. In addition, the auger-like blade is provided with a generally concave distal end ground to provide a generally fish-tailed appearance at its outer end terminating in two diametrically opposed sharp portions capable of effecting an axial boring cut. Finally, the surgical instrument is designed for very rapid cutting speeds. In many applications, including arthroscopic knee surgery and arthroscopic shoulder surgery a rotational speed for the auger-like helical cutter blade of about 3,000 rpm is used. The surgical instrument of the present invention is adaptable to the skill of the operating surgeon. A lower speed of 1,000 rpm is possible while upper rotational speeds of 10,000 rpm or even higher are attainable for delicate operations such as an operation on the human eye. FIG. 14 is a fragmentary perspective of another modified sheath member with a vertical cross section cut. Referring now to FIG. 1 of the drawings, there is illustrated a surgical instrument indicated generally at 20 with the instrument being inserted into a small puncture site below the patella of the knee joint of a patient P. Not shown in the figure are conventional elements including another small puncture site to receive a fiber optic device to light the interior of the joint from a suitable light source which return a visual image either directly to an eye piece for the surgeon or preferably with recording cameras to a visual display which is observed by the surgeon in performing the operation. Also not specifically illustrated is the external introduction of a saline solution through another small puncture site at the knee. A vacuum line hose 22 is illustrated attached to the surgical instrument and a pneumatic drive hose 24 drives a motor within motor assembly 78. Compressed nitrogen is usually used for this purpose. Alternatively, an electric motor can be used, although special precautions must be taken to ensure the safety of the patient, especially when saline solutions and the like are being employed. Reference to FIG. 2 illustrates a meniscus indicated generally at 26. This meniscus is generally C-shaped in cross section and is illustrated to have a tear at 28. This is only one of a great many different configurations that a damaged meniscus may have. In order to prevent the damaged meniscus tissue from being pinched between the bones comprising the knee joint, extraneous tissue will be removed by making a cut substantially along the dashed lines 30, 32 in order to provide a relatively smooth configuration for the tissue at the site of the meniscus. FIG. 3 illustrates in an exploded view, a number of the major elements of the surgical instrument. Thus, there is illustrated a suction housing member 34 which prior to being assembled is open at both ends as may be seen by an inspection of FIGS. 4 and 10. A cylindrical sheath member 36 is illustrated which is open at both ends and terminates at its distal end in a pair of longitudinally extending, diametrically opposed tabs 38. A vacuum tube 40 is secured to the suction housing member 34 at port 42 as seen in FIGS. 4 and 10. Vacuum tube 40 is provided with a series of hose barb seal members 44 in order to receive and retain the vacuum line hose 22. As can be seen best in FIGS. 4 and 10, the cylindrical sheath member 36 is provided with a sealing head plug member 46 inserted into one open end of the suction housing member 34. An auger-like helical cutter blade 48 received and supported by cutter housing member indicated generally at 50 is inserted within the cylindrical sheath member and resides in closely spaced relationship thereto. FIGS. 4 and 10 also illustrate the use of an O-ring on the cutter housing member 50 so as to provide a seal with the interior of the suction housing member 34 on the side of port 42 that is remote from the cylindrical sheath member 36. A C-shaped spring retainer 54 forms a longitudinal stop for an internal bearing 56. The helical cutter 48 is provided with a thickened end shaft 66 having hub ring members 58 thereon which straddle the bearing 56. A spacer member 60 with any required shim members are used to obtain the proper longitudinal spacing for the distal end of helical cutter 48 with respect to the tab members 38. An end seal member 62 is then placed next to the spacer and/or shim members. An additional constructional feature of the cutter blade includes a pin drive member 64 diametrically inserted through the thickened end shaft 66. A quick disconnect nut member 68 is carried or trapped on a cutter drive coupling nut member 70 by virtue of a raised boss 72 (FIG. 10) on the cutter drive coupling nut member 70. Left handed threads are provided at 74 on the cutter drive coupling nut member 70 and threaded on the exterior left hand threads 76 of motor assembly 78. A hose receptor 79 is provided for the motor assembly 78 at one end and the motor shaft 80 extends to the left at the other end in FIGS. 4 and 10. Screw 82 secures a cutter drive coupling member 84 to the shaft 80 of the motor. The cutter drive coupling member 84 is slotted at 86 and provided with a widely tapering end so as to self-seat the pin drive member 64 of the helical cutter blade 48. Spring 88 is retained within the cutter drive coupling member 84 by means of recess 90 in the cutter drive coupling member 84 and engages the end of the thickened end shaft 66 so as to urge the auger-like helical cutter blade 48 constantly toward its distal end eliminating any positional change caused by any backlash in the geared drive motor. In order to effect a quick disconnect for the surgical instrument and to gain access to the cutting blade, the quick disconnect nut member 68 is provided with internal threads 92 engageable with external threads 94 of the suction housing member 34. By simply unscrewing this connection, the end of the cutter housing member 50, which is knurled at 95 as illustrated in FIG. 3, is simply pulled away from the cylindrical sheath member whereby any jammed tissue may be removed. Alternatively, a different size of helioal cutter 48 and cylindrical sheath 36 may be inserted. Certain measures may be taken to minimize lodging of the tissue within the surgical instrument and these are shown in FIGS. 12-14 to include the application of straight grooves such as that shown in FIGS. 12 and 13 within the cylindrical sheath member 36 or the use of spiral grooves 96a within cylindrical sheath member 36a in FIG. 14. The direction of spiralling of the spiral grooves 96a is preferably opposite to that of the spiralling of the helix on the cutter blade 48. Further details of the distal end of the auger-like helical cutting blade 48 and cylindrical sheath member 36 may be seen in FIGS. 5-9. In particular, one longitudinally extending edge of each of the tabs 38 is ground to provide a cutting edge 100. The edge of the helical spiral on the helical cutter blades 48 cooperate with these cutting edges 100 to provide lateral shearing of tissue which is inserted laterally between the tabs 38. The tabs 38 are undercut at 102 to provide a protective outer stop shoulder or lip for the blade 48. With the aid of spacer member 60 and any required shims, the outer edge of the cutter blade 48 is positioned from about two to about four thousandths of an inch away from the hook-like undercut portion of the tab members 38. In addition, the auger-like helical cutting blade 48 is provided with a generally concave distal end at 104 that is ground to provide a generally fish-tailed appearance at its outer end terminating in two diametrically opposed sharp portions or types 106 so that the surgical instrument is capable of making what is called a plunge cut or an axial boring cut. To increase the stiffness of the surgical instrument, it is possible to provide increased thickness at the region of the tab members such as is shown at 108 in FIGS. 5 and 8. It is possible to vary the details of the blade cutter to suit the skill of the surgeon as well as to vary it to accommodate different tissues that are inserted between the tab members 38. For example, the back angle or sharp edge at 106 on the helical cutter blade 48 may be varied from about 35 degrees minimum to an angle approaching 90 degrees. If this angle is too blunt, no cutting will be effected and if it is too steep, the cutting edge becomes fragile. It is also possible to vary the width of the tab members 38 of the sheath. As the width is made narrower, it is possible to cut faster. This width may be either less than the diameter of the blade or greater than the diameter of the blade. Once again the width is varied dependent upon the skill of the surgeon. As far as the amount of suction is concerned, 27 inches of mercury is deemed to be high suction and will work very satisfactorily with the surgical instrument of the present invention. Twelve to 16 inches of mercury is considered to be marginal and if the suction is less than that, the chances of clogging the spiral groove in the auger-like helical cutter blade 48 becomes much greater. The auger-like blade with its helical groove serves as a conduit for severed tissue under the influence of the vacuum connected to vacuum tube 40. While presently preferred embodiments of the invention have been illustrated and described, it will be recognized that the invention may be otherwise variously embodied and practiced within the scope of the claims which follow. (3) said helical cutter blade further having at least a portion of its distal end ground to provide a concave distal end providing a generally fish tailed appearence terminating in two diametrically opposed sharp tynes so that the instrument is capable of making an axial boring cut and wherein the length of the concavity of said concave distal end in a direction along the longitudinal axis of said cylindrical sheath is less than the length of said tabs. 2. A hand held surgical instrument as defined in claim 1 wherein the grinding of said helical cutter to produce two diametrically opposed sharp portions to produce axial boring cuts forms an angle of at least 35 degrees with an axis parallel to the longitudinal axis of said helical cutter. 3. A hand held surgical instrument as defined in claim 1 wherein said cylindrical sheath is internally grooved to assist in the removal of severed tissue. 4. A hand held surgical instrument as defined in claim 1 wherein the distal end of said sheath is thickened. 5. In a hand held surgical instrument as defined in claim 1, wherein said motor drive rotates said cutter blade at a rotational speed of at least 1000 rpm. (3) said helical cutter blade further having at least a portion of its distal end ground to provide a concave distal end providing a generally fish tailed appearance terminating in two diametrically opposed sharp tynes, each of said tynes presenting a generally radially extending cutting edge so that the instrument is also capable of making an axial boring cut independently of said external tabs and wherein the length of the concavity of said concave distal end in a direction along the longitudinal axis of said cylindrical sheath is less than the length of said tabs. 4203444 May 20, 1980 Bonnell et al. 4274414 June 23, 1981 Johnson et al.

Yasmine Galenorn is a New York Times, Publishers Weekly, and USA Today bestselling author and writer of paranormal romance, urban fantasy, and science fiction. In the past, she has written paranormal mysteries and nonfiction metaphysical books. With over fifty books on the shelves, she is the 2011 Career Achievement Award Winner in Urban Fantasy, given by RT Book Reviews. She lives in Kirkland, Washington, with her husband Samwise and their cats.

Acantothereva rungsi är en tvåvingeart som beskrevs av Eugène Séguy 1935. Acantothereva rungsi ingår i släktet Acantothereva och familjen stilettflugor. Artens utbredningsområde är Marocko. Inga underarter finns listade i Catalogue of Life. Källor Stilettflugor rungsi

Lexicon Valley is a podcast for word nerds. Truly. It's all about language– etymology, syntax, usage, how a language develops or dies. There is occasional explicit material (of the linguistic variety), but I recommend this for word nerds or grammar geeks. It is one of my most favorite things to listen to! The Tobolowsky Files is a podcast that features Stephen Tobolowsky telling his life stories. I've never heard such detail in memories! He's a gifted storyteller and weaves stories from different parts of his life together to reveal a truth or a lesson or simply to show connections. I'm sad when there aren't new episodes of this one. White Horse Inn is a podcast centered around knowing what you believe and why you believe it. It includes in-depth conversations, interviews, and discussions on everything from Islam to worship to our scatterbrains. It's a little theology-geeky, but really solid in its content. This American Life is the greatest storytelling available in audio format. Truly incredible. Each hour long episode follows a theme or idea and has stories in various formats– personal accounts, reports, fiction. We often don't agree with the content, but we look past it because of the amazing storytelling. "The Briefing enables Christians to think biblically by providing daily worldview analysis about the leading news headlines and cultural conversations." Basically, it's a 15 minute news analysis from a solid theological perspective. Albert Mohler is president of the Southern Baptist Theological Seminary, and one of the leading minds of our time. It's pretty amazing and I think I may have a tiny crush on Mr. Mohler. Must be the bow ties. This entry was posted in Marriage, Our First Year and tagged internet, podcasts by Lindsey. Bookmark the permalink.

Home → Cadillac Cadillac Pictures Browse through our rich database of Cadillac Pictures models available for download in different image resolutions, including full HD and retina. Cadillac announced a unique project in which its all-new luxury crossover, the XT5, will debut at venues around the world in partnership with acclaimed fashion designers Public School. At a private kickoff event, Public School announced it would debu 2016 Cadillac ATS-V Coupe Racecar The next generation of Cadillac's V-Series elite, high-performance cars began today with the introduction of an all-new race car, the Cadillac ATS-V.R. Set to begin competition in 2015, Cadillac debuted the new race car at the Circuit of the Ameri 2016 Cadillac ATS-V Sedan Cadillac today introduced the 2016 ATS-V, expanding the acclaimed ATS product range and elevating the brand's elite high-performance V-Series to its next generation. Available in sedan and coupe forms, the first-ever ATS-V will introduce c 2016 Cadillac ATS-V Coupe Cadillac introduced the 2016 ATS-V, expanding the acclaimed ATS product range and elevating the brand's elite high-performance V-Series to its next generation. Available in sedan and coupe forms, the first-ever ATS-V will introduce class-l Cadillac today introduced the 2016 CTS-V, the most powerful product in the brand's 112-year history and the newest entry in the elite class of the world's highest-performing luxury cars. Launching in late summer with 640 horsepower (477 kW 2016 Cadillac CT6 Cadillac held the world premiere of the 2016 CT6, elevating it to the top of its range and creating a new formula for the prestige sedan through the integration of new technologies developed to achieve dynamic performance, efficiency and agility p Cadillac today announced the ELR electrified luxury coupe will offer a host of upgrades for the 2016 model year, including boosts in acceleration, driving performance and connectivity. Major product upgrades include a more than 25 percent Cadillac today introduced the all-new 2015 Escalade, a sophisticated luxury SUV designed to establish new benchmarks for hand-tailored craftsmanship and technology. The fourth-generation Escalade is an entirely new design yet instantly re 2015 Cadillac ATS Coupe Cadillac today introduced the all-new 2015 ATS Coupe, a quicker and more personal addition to the award-winning ATS product line. Designed to be lighter, more agile and more engaging than its competitors, it extends Cadillac's product-driven growt 2015 Cadillac CTS-V Coupe Cadillac today announced production of a limited, special-edition 2015 CTS-V Coupe, marking the last chapter of the current model before the next generation of the V-Series debuts next year. The 2015 CTS-V Coupe will consist of 500 limited Cadillac unveils the 2014 ELR today at the North American International Auto Show. The sleek luxury coupe features the first application of Extended Range Electric Vehicle technology by a full-line luxury automotive brand. Cadillac today introduced the all-new 2014 CTS sedan, which ascends into the heart of the midsize luxury market with expanded performance, elevated luxury and sophisticated technology. The third-generation CTS sedan is based on the high-pe

Q: Transferring MySQL Workbench to Server I am trying to move my database model from mysql workbench to mysql server. I am using the reverse engineer sql create script but when importing it gives me an error. I have tried to google the problem but no luck to my situation. -- ----------------------------------------------------- -- Table `MapLibrary`.`Books` -- ----------------------------------------------------- DROP TABLE IF EXISTS `MapLibrary`.`Books` ; CREATE TABLE IF NOT EXISTS `MapLibrary`.`Books` ( `ISBN` VARCHAR(13) NOT NULL, `date_of_publication` INT NULL, `book_title` VARCHAR(45) NULL, PRIMARY KEY (`ISBN`)) ENGINE = InnoDB; -- ----------------------------------------------------- -- Table `MapLibrary`.`Genre` -- ----------------------------------------------------- DROP TABLE IF EXISTS `MapLibrary`.`Genre` ; CREATE TABLE IF NOT EXISTS `MapLibrary`.`Genre` ( `genre_code` INT NOT NULL, `genre_name` VARCHAR(45) NOT NULL, PRIMARY KEY (`genre_code`)) ENGINE = InnoDB; -- ----------------------------------------------------- -- Table `MapLibrary`.`Books_By_Genre` -- ----------------------------------------------------- DROP TABLE IF EXISTS `MapLibrary`.`Books_By_Genre` ; CREATE TABLE IF NOT EXISTS `MapLibrary`.`Books_By_Genre` ( `genre_code` INT NOT NULL, `ISBN` VARCHAR(13) NOT NULL, PRIMARY KEY (`genre_code`, `ISBN`), INDEX `Books_idx` (`ISBN` ASC), CONSTRAINT `Books` FOREIGN KEY (`ISBN`) REFERENCES `MapLibrary`.`Books` (`ISBN`) ON DELETE NO ACTION ON UPDATE NO ACTION, CONSTRAINT `Genre` FOREIGN KEY (`genre_code`) REFERENCES `MapLibrary`.`Genre` (`genre_code`) ON DELETE NO ACTION ON UPDATE NO ACTION) ENGINE = InnoDB; ERROR: ERROR 1022 (23000) at line 89: Can't write; duplicate key in table 'books_by_genre' Operation failed with exitcode 1 A: The problem is that the ISBN is NOT a unique identifier on its own in the referenced tables. I assume, that the Books table is your base table and the three referenced tables are M:N link tables. If this is true, then your foreign keys are pointing in the wrong direction. (Try to drag the connection in the other direction?)

QUOTES BOOK TALK Front Cover | Back Cover Waiting for a Name poems by Elizabeth Jaikaran Print (softcover) $17.95 ORDER NOW FOR SHIPMENT IN MID-JANUARY To pay by check please use this order form. We are pleased to take orders from retailers. Email us with details about your order or call us at 207-837-5760. Waiting for a Name is a collection of poetry devoted to the questions surrounding love, divinity, and identity. It is an exploration of the magic contained in the Caribbean diaspora. It is the veneration of the imperfect Muslim. It is one of the many war cries of Queens, New York. POETRY / General POETRY / Subjects & Themes / Family POETRY / Women Authors ISBN: 978-1-956056-61-7 (print; softcover; perfect bound) copyright 2023; released 1-17-2023 Elizabeth Jaikaran is a New York–based author and lawyer, with work published across a spectrum of print and digital media. She began writing at the age of eight and, since then, has published fiction, non-fiction, legal commentary, poetry, and comedy. She is the child of Guyanese immigrants, born in Brooklyn, New York, and raised in Queens. Jaikaran's writing has appeared in the Huffington Post, Playboy, The Higgs Weldon, Brown Girl Magazine, Drunk Magazine, SILENT Magazine, Literally, Darling Magazine, The Muslim Observer, Bruk Out Media, PREE Lit, Human/Kind Journal, and Defunkt Magazine. Her first book, Trauma: A Collection of Short Stories, was published by Shanti Arts in 2017, and is recognized as a critically acclaimed work. Waiting for a Name is her first collection of poetry. "What does it mean to have a name? Half pronounced, mispronounced, uncertain, unofficial? What if we are named by all the things that compose us as beings? What if waiting to be named is the approach? Waiting for a Name is an ode to the multi-faceted self and its incarnations. It is in the specificity of Queens, Richmond Hill, Guyanese Creole, combined heritages, passings and goodbyes, matriarchal warnings, dreams and desires, languages dictated and pronounced. It is a revelation. It is love. May we all find tenderness and healing among these pages as Elizabeth Jaikaran has gifted us in these life-affirming words." —Sokhunthary Svay, author of Apsara in New York: Poems "I am touched, moved, and forever changed—Liz Jaikaran's poetry collection is as powerful as it is necessary. Jaikaran's power with the pen brings to life complex themes of diaspora and home, love and trauma, life and death with ease and care. Her gift with writing, and especially this form, allows the reader to feel seen, while also being keenly aware that we're seeing Jaikaran stand tall among the lessons and blessings of her ancestors. This collection is a must-read for all those who have ever felt misunderstood, far from home, or painfully alone—namely, it is a must-read for all human beings" —Priya Arora, host of Queering Desi Articles and Reviews Shanti Arts LLC. Copyright © 2011-2023. All rights reserved. Content may not be reproduced in any form. info@shantiarts.com | 193 Hillside Road Brunswick Maine 04011 | 207-837-5760

Q: Calculating p-values from a tbl_merge object? I have been using the R function gtsummary, but have now run into a problem with variables existing in subsets of each other. I have two screening tests that can independently be positive of one another which has created an issue with using by = with tbl_summary. This lead me to create two separate tables and combine them with the tbl_merge function. However, I would like to calculate p-values for differences between the tests. Is there any way to do this with an object in the table_merge format or to extract information from it and calculate the statistics I need? Per request, I post a code example from the mtcars database. Example code ------------------------------------------------------------ data(mtcars) mtcars_tab <- mtcars %>% mutate( drat_4 = ifelse(drat >= 4, 1, 0), wtp_3 = ifelse(wt >= 3, 1, 0), testp = ifelse(drat_4 == 1 | wtp_3 == 1, 1, 0)) %>% select(testp,vs,am) tab <- tbl_summary(mtcars_tab, missing = "no", by = testp ) %>% add_p tab Table 1 What I would like is the table to split into one column where drat_4 is positive and another column where wtp_3 is positive, but I'm having a hard time with this since they are overlapping. Additionally, I would then compare the variables for the positive cases of drat_4 and wtp_3 and add p-values to the table. Sorry if this is confusing. I'll try to clarify! Would really appreciate your help with this! A: I think the code example solves your problem. You'll need to supplement the code with a proper comparison of the two columns that takes into account that the two groups are not mutually exclusive. library(gtsummary) library(tidyverse) packageVersion("gtsummary") #> [1] '1.4.0.9004' mtcars_tab <- mtcars %>% mutate( drat_4 = ifelse(drat >= 4, 1, 0), wtp_3 = ifelse(wt >= 3, 1, 0) ) %>% select(drat_4, wtp_3, cyl, mpg) with(mtcars_tab, table(drat_4, wtp_3)) #> wtp_3 #> drat_4 0 1 #> 0 6 19 #> 1 6 1 # table among drat_4 test positive tbl1 <- mtcars_tab %>% filter(drat_4 == 1) %>% tbl_summary( missing = "no", type = mpg ~ "continuous", include = -c(drat_4, wtp_3) ) # table among wtp_3 test positive tbl2 <- mtcars_tab %>% filter(wtp_3 == 1) %>% tbl_summary( missing = "no", type = mpg ~ "continuous", include = -c(drat_4, wtp_3) ) # create a table with the variable and the p.value tbl_with_pvalue <- tibble( variable = c("cyl", "mpg"), row_type = "label", # you'll need to write a proper function here to get the p-values from an approrate test p.value = c(0.033, 0.044) ) tbl_final <- # merge the two tables tbl_merge(list(tbl1, tbl2)) %>% # add a pvalue comparing the two columns modify_table_body( ~.x %>% dplyr::left_join( tbl_with_pvalue, by = c("variable", "row_type") ) ) %>% # add header for new pvalue column modify_header(p.value ~ "**p-value**") %>% # add a formatting function for p-value modify_fmt_fun(p.value ~ style_pvalue) Created on 2021-05-06 by the reprex package (v2.0.0)

Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein Tetsuji Yamaguchi, Toshiyuki Yamada, Ryuta Okutani, Noriharu Shijubo, Gurmukh Singh, Yoshihisa Itoh Human protein 1/ Clara cell Mr 10,000 protein consists of two identical subunits of seventy amino acid residues each. In the present study, eight clones of monoclonal antibodies against native protein 1 were prepared and their respective epitopes were immunochemically and immunohistochemically characterized using native protein I, truncated recombinant protein 1 and synthesized peptides. Among the clones, three designated as TY-5, TY-7 and TY-8 recognized amino acid residues 7-16, residues 19-28, and residues 39-46, respectively, all of which comprise the hydrophobic cavity of protein 1, possibly associated with chemical binding function. With the exception of TY-4, the remaining clones recognized residues 61-68 which are exposed to solvent. The epitope of TY-4 remains undetermined. Proper selection and combination of clones and recombinant protein 1 may be useful for fundamental and clinical studies of protein 1. Clinical Chemistry and Laboratory Medicine https://doi.org/10.1515/CCLM.1999.098 Clara cell 10 kilodalton protein Epitope Human protein 1 Truncated recombinant protein Biochemistry, medical 10.1515/CCLM.1999.098 Fingerprint Dive into the research topics of 'Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein'. Together they form a unique fingerprint. Clone Cells Medicine & Life Sciences Recombinant Proteins Medicine & Life Sciences Epitopes Medicine & Life Sciences Amino Acids Medicine & Life Sciences Clinical Studies Medicine & Life Sciences Peptides Medicine & Life Sciences Yamaguchi, T., Yamada, T., Okutani, R., Shijubo, N., Singh, G., & Itoh, Y. (1999). Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein. Clinical Chemistry and Laboratory Medicine, 37(6), 631-637. https://doi.org/10.1515/CCLM.1999.098 Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein. / Yamaguchi, Tetsuji; Yamada, Toshiyuki; Okutani, Ryuta; Shijubo, Noriharu; Singh, Gurmukh; Itoh, Yoshihisa. In: Clinical Chemistry and Laboratory Medicine, Vol. 37, No. 6, 01.09.1999, p. 631-637. Yamaguchi, T, Yamada, T, Okutani, R, Shijubo, N, Singh, G & Itoh, Y 1999, 'Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein', Clinical Chemistry and Laboratory Medicine, vol. 37, no. 6, pp. 631-637. https://doi.org/10.1515/CCLM.1999.098 Yamaguchi T, Yamada T, Okutani R, Shijubo N, Singh G, Itoh Y. Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein. Clinical Chemistry and Laboratory Medicine. 1999 Sep 1;37(6):631-637. https://doi.org/10.1515/CCLM.1999.098 Yamaguchi, Tetsuji ; Yamada, Toshiyuki ; Okutani, Ryuta ; Shijubo, Noriharu ; Singh, Gurmukh ; Itoh, Yoshihisa. / Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein. In: Clinical Chemistry and Laboratory Medicine. 1999 ; Vol. 37, No. 6. pp. 631-637. @article{984cf707697248ecacedbc24c7aa88d0, title = "Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein", abstract = "Human protein 1/ Clara cell Mr 10,000 protein consists of two identical subunits of seventy amino acid residues each. In the present study, eight clones of monoclonal antibodies against native protein 1 were prepared and their respective epitopes were immunochemically and immunohistochemically characterized using native protein I, truncated recombinant protein 1 and synthesized peptides. Among the clones, three designated as TY-5, TY-7 and TY-8 recognized amino acid residues 7-16, residues 19-28, and residues 39-46, respectively, all of which comprise the hydrophobic cavity of protein 1, possibly associated with chemical binding function. With the exception of TY-4, the remaining clones recognized residues 61-68 which are exposed to solvent. The epitope of TY-4 remains undetermined. Proper selection and combination of clones and recombinant protein 1 may be useful for fundamental and clinical studies of protein 1.", keywords = "Clara cell 10 kilodalton protein, Epitope, Human protein 1, Monoclonal antibody, Truncated recombinant protein", author = "Tetsuji Yamaguchi and Toshiyuki Yamada and Ryuta Okutani and Noriharu Shijubo and Gurmukh Singh and Yoshihisa Itoh", doi = "10.1515/CCLM.1999.098", journal = "Clinical Chemistry and Laboratory Medicine", publisher = "Walter de Gruyter GmbH & Co. KG", T1 - Characterization of monoclonal antibodies to human protein 1/Clara cell 10 kilodalton protein AU - Yamaguchi, Tetsuji AU - Yamada, Toshiyuki AU - Okutani, Ryuta AU - Shijubo, Noriharu AU - Singh, Gurmukh AU - Itoh, Yoshihisa N2 - Human protein 1/ Clara cell Mr 10,000 protein consists of two identical subunits of seventy amino acid residues each. In the present study, eight clones of monoclonal antibodies against native protein 1 were prepared and their respective epitopes were immunochemically and immunohistochemically characterized using native protein I, truncated recombinant protein 1 and synthesized peptides. Among the clones, three designated as TY-5, TY-7 and TY-8 recognized amino acid residues 7-16, residues 19-28, and residues 39-46, respectively, all of which comprise the hydrophobic cavity of protein 1, possibly associated with chemical binding function. With the exception of TY-4, the remaining clones recognized residues 61-68 which are exposed to solvent. The epitope of TY-4 remains undetermined. Proper selection and combination of clones and recombinant protein 1 may be useful for fundamental and clinical studies of protein 1. AB - Human protein 1/ Clara cell Mr 10,000 protein consists of two identical subunits of seventy amino acid residues each. In the present study, eight clones of monoclonal antibodies against native protein 1 were prepared and their respective epitopes were immunochemically and immunohistochemically characterized using native protein I, truncated recombinant protein 1 and synthesized peptides. Among the clones, three designated as TY-5, TY-7 and TY-8 recognized amino acid residues 7-16, residues 19-28, and residues 39-46, respectively, all of which comprise the hydrophobic cavity of protein 1, possibly associated with chemical binding function. With the exception of TY-4, the remaining clones recognized residues 61-68 which are exposed to solvent. The epitope of TY-4 remains undetermined. Proper selection and combination of clones and recombinant protein 1 may be useful for fundamental and clinical studies of protein 1. KW - Clara cell 10 kilodalton protein KW - Epitope KW - Human protein 1 KW - Monoclonal antibody KW - Truncated recombinant protein U2 - 10.1515/CCLM.1999.098 DO - 10.1515/CCLM.1999.098 JO - Clinical Chemistry and Laboratory Medicine JF - Clinical Chemistry and Laboratory Medicine

has led a $60 million investment in Betterment ("Betterment", or the "Company), is an online investment adviser providing investment advice and diversified, fully automated investment management to customers for less than the typical cost of a traditional financial adviser or wealth manager. The company was founded in August 2008 and launched in May 2010 at TechCrunch Disrupt, where it was named "Best Start Up in New York City." The company is headquartered in New York, NY. Betterment's investments are not share-based, but rather goal-based; it diversifies every penny invested through proprietary software, and deploys dollar cost averaging. It is a portfolio comprised of the most efficient ETFs, allowing for liquidity. They currently manage over $1.4B of assets. Marlin & Associates advised global private equity firm Francisco Partners on strategy and valuation on their investment, resulting in a $60M commitment. Francisco Partners led the financing, which includes participation from previous investors Bessemer Venture Partners, Menlo Ventures, and Northwestern Mutual Capital. Francisco Partners specializes in investments in technology companies. Since its launch fifteen years ago, FP has raised approximately $10 billion and invested in more than 150 technology companies, making it one of the most active investors in the industry. The firm invests in transaction values ranging from $50 million to over $2 billion. Marlin advised Francisco Partners on its investment in Betterment. Marlin provided detailed insight into the space, competitors, and potential follow-on acquisition opportunities. Betterment Raises $60M in Growth Funding New Round Will Accelerate Growth and Product Development NEW YORK, Feb. 19, 2015 /PRNewswire/ -- Betterment, the largest and fastest growing automated investing service, today announced the close of a $60 million round of growth funding. Global private equity firm Francisco Partners led the financing, which includes participation from previous investors Bessemer Venture Partners, Menlo Ventures, and Northwestern Mutual Capital. "Our growth has continued to accelerate," said Jon Stein, Betterment Founder and CEO. "More people are becoming Betterment customers every day, and our existing customers continue to invest more with us. This new capital will allow us to grow even faster and increase the development of new products that will continue to reinvent investing around what customers want: a seamless, personalized experience that is aligned with their best interest and optimized for the highest expected returns net of all costs." Launched in 2010, Betterment manages more than $1.4B of assets in tax-efficient, personalized portfolios for more than 65,000 customers, by far the largest customer base of any automated investing service. The company offers a seamless, technology-enabled platform that helps people better manage, protect, and grow their wealth. "We've created a new product category over the last five years," Stein added. "While we're excited with where we are today, we're really just getting started. There are millions of people in need of better financial advice and services, and we're building the smartest technology and making it accessible to anyone." In addition to raising the new round of funding, Betterment is welcoming Peter Christodoulo of Francisco Partners to its Board of Directors. Francisco Partners brings significant experience in financial technology, with previous investments that include Prosper Marketplace, eFront, PayLease, Paymetric, Avangate and Hypercom among others. "We have long felt that the wealth management space was overdue for a solution that could better serve the majority of Americans through smarter technology," Christodoulo said. "Betterment's unique, vertically integrated architecture allows its platform to be lightning fast, which is proving to be compelling with customers as thousands fund new accounts each month." In the past year, Betterment has unveiled a variety of new features to help improve investor returns, including Tax Loss Harvesting+TM and Tax Impact Preview. The company also recently unveiled Betterment Institutional, a digital solution that allows financial advisors to better serve their clients and make their practices more efficient. For more information, please visit www.betterment.com and follow @Betterment on twitter. About Betterment Betterment is the largest automated investing service, helping people to better manage, protect, and grow their wealth through smarter technology. The service offers a personalized, goal-based, globally diversified portfolio of ETFs, designed to help provide you with the best possible expected returns for retirement planning, building wealth, and other savings goals. Betterment is a CNBC Disruptor 50 and Webby award winner and has been featured in the New York Times, Forbes, and the Wall Street Journal. Betterment helps people to achieve a smarter financial future with minimal effort and for a fraction of the cost of traditional financial services. About Francisco Partners Francisco Partners is a global private equity firm that specializes in investments in technology companies. Since its launch fifteen years ago, FP has raised approximately $10 billion and invested in more than 150 technology companies, making it one of the most active investors in the industry. The firm invests in transaction values ranging from $50 million to over $2 billion, where the firm's deep sub-sector knowledge and operational expertise can help a company realize its full potential.

iPads are being used for a wide range of activities across all subjects. We have found Pinterest to be a valuable way of researching and collecting ideas for Art, Textiles and Photography whether it be imagery to inspire projects, ideas of materials and techniques or finding examples of work. We use this app for students and teachers to document ideas and discussions with their teachers about their Art, Textiles and Photography work. It's a great app for collating information and imagery together. This is our favourite drawing app. It is versatile, allowing imagery to be layered up, allowing you to draw over photographs using a variety of tools. This app allows you to locate places as well as zoom in to look at the landscape and settlement size. World figures is great as it contains statistics on every country in the world and is very useful for comparing the development of different countries. Skitch is a very useful app as it allows you to annotate a photo and analyse the geographical features. This app is specific to the OCR exam unit. This is a great revision tool when revising for the exam. Hopscotch is used for KS3 ICT to help students learn programming. The students can grasp the basic concepts of programming including repeat loops. We use GoodNotes for all notetaking in lessons. We have found it a great way for students to organise their work. This is a theory based app with quizzes, debates and informative overviews. This is particularly useful tool for revising key terms and concepts. When students are doing "to camera" pieces this is an invaluable app which does the job of a real Teleprompter brilliantly! A fantastic app for ensuring that scripts and storyboards can be constructed according to industry practice. It has pre loaded instructions to ensure that students are guided on the exact process of layout / design features. This chemistry app lets you see what each element does individually and goes into detail on how some of them can react together and what they produce. This app provides an interactive way to see and show functions of the body from digestion to brain function. This is helpful for years 7-11. This app provides an interactive way to place electrons, protons and neutrons on each element. This is very helpful to those studying atoms for the first time. Students can use GoodNotes for notetaking in lessons which enables them to organise their work efficiently.

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In May 2017 we were invited to meet with BMT Hi-Q Sigma to discuss developing their branding. design146 had previously worked with the client at Altus and were keen to be involved. BMT Hi-Q Sigma is a Management Consultancy, based in Bath, providing services across Government, Defence, Energy and Transport sectors to improve their portfolio, programme and project performance. They already have a set of existing brand guidelines, but needed some help to develop them further to show how they might be adapted to more flexible layouts that are efficient on space. To kick things off, we have worked on a branding development presentation and some Case Studies and White Papers. We're looking forward to seeing how the branding develops as we work on other marketing collateral to include infographics, branded merchandise, and internal comms pieces.

Voice acting is the art of doing voice overs or providing voice to represent a character. Your favourite cartoons get all their voices from voice actors. The Mickey Mouse, Naruto, Goku, all are voices of actual people working behind the scenes. Must a compelling voice to make the listeners feel the moments. Earnings are extremely flexible in this industry and there is no exact earning that can be listed. The more valuable the projects you are assigned to, the more money you will earn.

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He is one of the most reputed Cardiologists in the "City of Pearls'' having more than 18 years' experience Know More.. Dr. V K Srinagesh, MS M Ch. Cosmetic & Plastic Surgeon, Days: Mon-Sat, Timings: 6 P.M. – 8 P.M. Dr. V. Kameswara Srinagesh is a Cosmetic and Plastic Surgeon at Shenoy Hospitals. He is one the seasoned Cosmetic and Plastic Surgeon Know More.. Dentistry, Days: Mon-Sat, Timings: 9 A.M. – 9 P.M. Dr. Satya Narayana is a Dentist at Shenoy Hospitals. He is one of the renowned Dentist in the City of Pearls with an amazing experience of 25 years Know More.. Dermatologist, Days: Mon – Sat, Timings: 9 A.M. – 11 A.M. Dr.Kotla Sai Krishna is Consultant Dermatologist & Cosmetic Surgeon at Shenoy Hospitals. He is one of the best consultant Know More.. Dermatologist, Days: Mon, Tue, Thur, Fri & Sat, Timings: 1:30 P.M. – 2:30 P.M. Dr. Satyanarayana Reddy is a Dermatologist at Shenoy Hospitals, specializing in skin, hair, nail and sexual problems Know More.. Diabetologist, Days: Mon – Sat, Timings: .3 P.M. – 4 P.M. Dr. Pradnya Kamble is a Diabetologist at Shenoy Hospitals. She is a captivating doctor with a dynamic personality in Hyderabad Know More.. ENT Specialist, Days: Mon – Sat, Timings: 10 A.M. – 6 P.M. Dr.D.Vidyasagar is a Consultant ENT & HNS at Shenoy Hospitals. He has a vast experience of more than 11 years and is one of the leading Know More.. ENT Surgeon, Days: Mon – Fri, Timings: 5:00 P.M. – 7:30 P.M. Dr. Vyshanavi Bommakanti is an ENT Surgeon at Shenoy Hospitals. She is one the most well known ENT Surgeons in the city of Hyderabad Know More.. Fetal Medicine Specialist, Days: Tue, Timings: 9:30 A.M. – 2:00 P.M. Dr. Chinmayee Ratha is an Obstetrician and Gynecologist at Shenoy Hospitals. She is a captivating doctor in this City of Pearls. Know More.. Gastroenterologist, Days: Mon-Sat (On Call), Timings: 6 P.M. – 7 P.M. Dr Ramanjaneyulu Erukulla is Gastroenterologist at Shenoy Hospitals. He graduated from Osmania Medical College in 1990 and has the distinction Know More.. General Physician, Days: Mon, Wed, Fri & Timings: 3 P.M. – 5 P.M. Dr. Shyam Sunder is a General Physician at Shenoy Hospitals. He is one of the renowned General Physician in Secunderabad and Hyderabad Know More.. General Physician, Days: Mon-Sat, Timings: 10 A.M. – 1 P.M. Dr. Mohammed Parvez is a General Physician at Shenoy Hospitals. He is American Board Certified in Internal Medicine. He has completed Residency Know More.. General Physician, Days: Mon-Sat, Timings: 5 P.M. – 7 P.M. Dr. Aishwarya, M.D., is a General Physician specialist in Shenoy Hospitals. She has 7 years of experience in the field of General MedicineKnow More.. General Surgeon, Days: Mon & Thur (On Call), Timings: 12 P.M. – 1 P.M. Days: Tue, Wed, Fri & Sat, Timings: 9A.M. – 12 P.M. Haemotologist, Day: Sat, Timings: 3 P.M. – 6 P.M. Dr. Aarti is a well-known Fertility Specialist in Hyderabad, with an experience of more than two decades in this field Know More.. Dr. Navatha Mortha is a Maxillofacial Surgeon at Shenoy Hospitals. A very well-known Surgeon in the twin cities of Hyderabad and Secunderabad Know More.. Nephrologist, Days: Mon-Sat (On Call), Timings: 2:00 P.M. – 4:00 P.M. Dr. Archana Daftardar is a Nephrologist at Shenoy Hospitals, with expertise in Clinical Nephrology, Dialysis, and Renal Transplantation Know More.. Neurologist, Days: Mon-Sat, Timings: 4 P.M. – 5 P.M. Dr. Gopal Poduval is a Neurologist at Shenoy Hospitals. He is one of the best Neurologists in the cities of Hyderabad and Secunderabad, having Know More.. Neurosurgeon & Endoscopic Spine Surgeon, Days: Mon – Fri, Timings: 6:00 P.M. – 8:00 P.M. Dr. Kalyan Bommakanti is a Neurosurgeon & Endoscopic Spine Surgeon at Shenoy Hospitals. He has more than 10 yrs experience Know More.. Nutritionist, Days: Mon-Sat, Timings: 11:00 A.M. – 5:00 P.M. Dr.Sowmya is a Consultant Nutritionist in Shenoy Hospitals. She holds an experience of 8 years as a Nutritionist Know More.. Obstetrician & Gynecologist, Days: Mon – Sat, Timings: 10 A.M. – 3 P.M. Dr. Kadambari is an Obstetrist and Gynecologist at Shenoy Hospitals. She has a vast experience of 37 years in the field of Know More.. Obstetrician & Gynecologist, Days: Wed & Sat, Timings: 3 P.M. – 5 P.M. Dr.Prabha Mohan is a Gynecologist at Shenoy Hospitals. She has a vast experience of 24 years. She is a well-known doctor Know More.. Obstetrician & Gynecologist, Days: Mon – Sat, Timings: 1:30 P.M. – 6:00 P.M. Obstetrician & Gynecologist, Days: Mon -Sat (On Call), Timings: 6:00 P.M. – 8:00 P.M. Dr.Gitanjali.G is a Gynecologist at Shenoy Hospitals. She is one of the young & dynamic doctors of Hyderabad. She has completed her graduationKnow More.. Obstetrician & Gynecologist, Days: Sat, Timings:10 A.M. – 1:30 P.M. Dr. Aruna Rachel is an Obstetrist and Gynecologist at Shenoy Hospitals. She has a marathon experience of 30 years in the field of Know More.. Obstetrician & Gynecologist, Days: Wed-Sat, Timings: 10 A.M. – 6 P.M. Orthopaedic Surgeon, Days: Mon – Sat, Timings: 6 P.M. – 8 P.M. Dr. Ratnakar Rao K is a Consultant Orthopaedic Surgeon at the Shenoy Hospitals. He holds a rich experience of 10 years in the field of Orthopaedics Know More.. Orthopaedic Surgeon, Days: Mon – Sat, Timings: 6:30 P.M. – 7:30 P.M. Dr. Ravi Teja Rudraraju is an Orthopaedic Surgeon at Shenoy Hospitals, specializing in Traumatology and Reconstruction of Hip and Knee Know More.. Oncologist, Days: Tue, Thur & Fri, Timings: 5 P.M. – 6 P.M. Paediatrician, Days: Mon – Sat (On Call), Timings: 7 P.M. – 9 A.M. Dr Karthik is a Neonatologist at Shenoy Hospitals. He is one of the reputed Neonatologist in Secunderabad and Hyderabad Know More.. Pediatrician & Neonatologist, Days: Mon – Sat (On Call), Timings: 4 P.M. – 7 P.M. Dr. D.Suchitra is a Pediatrician at Shenoy Hospitals. She has an experience of 6 years in examining children and treating problems related Know More.. Dr. Vinay Kumar is a Pediatrician at Shenoy Hospitals. His has 4 years of experience in the field of pediatric and neonatology in Know More.. Days: Mon, Tue, Wed, Sat, Timings: 4 P.M. – 6 P.M. Days: Thur & Fri, Timings: 5 P.M. – 6 P.M. Dr.Tapaswi Krishna is a Pulmonologist at Shenoy Hospitals. She is one of the leading Pulmonologist with a vast experience Know More.. Radiologist / Sonologist, Days: Thur, Timings: 8:30 A.M. – 9:30 A.M. Dr.T.L.N.Praveen is a Radiologist at Shenoy Hospitals. He is a one of the established Radiologist in Secunderabad and Hyderabad Know More.. Radiologist / Sonologist, Days: Mon & Thur, Timings: 3 P.M. – 4 P.M. Vascular Surgeon, Days: Mon-Sat, Timings: 9 A.M. – 10 A.M. & 8 P.M. – 9 P.M.

Gotta Go! Title:Gotta Go! - Bedtime Shelly Jasperson "Don't drink all of that, you'll have to go during the movie." Brian didn't listen to his mother, but continued to gulp down his soda with vigor. And out of spite, he went and filled his cup again. His mother just sighed and prepared for the worst. Brian was in one of his moods. On the way to the theater, Brian's mom watched him pull faces at her through the rear-view mirror. Brian should have known she could see him. "Brian, don't do that or your face will get stuck like that." The comment caught Brian off guard, but he continued sticking out his tongue, rolled his eyes, and puffing up his chubby cheeks. They reached the theater and Brian ran around outside as his mother bought the tickets to Super Ninja Giraffes. "Slow down, Brian." She called before he bounced off of a man's leg. "Sorry about that." She apologized. Brian's mom took his hand, pulled him through the doors, and into the freezing theater. Brian ran to the concessions counter and ordered a large tub of double butter popcorn. His mouth watered as he watched the tall man pump butter into the big carton. When the man put it on the counter, Brian's mom apologized and took Brian down a long dark hallway without the popcorn. "Mom. . ." She pulled him inside a door and Brian could see the large screen with commercials printed in many different colors; he ran to the front row and sat down. His mother followed, but instead of sitting down, she stood next to him with her hands on her hips. "If you sit on the front row, you'll hurt your eyes and need glasses." But Brian wouldn't budge so his mom went to sit in the back without him. Soon Super Ninja Giraffes were running across the screen and fighting evil villains like the Red Eyed Robin and the Horrid Horned Hippo. And just when one of the mighty giraffes got caught and tied up, Brian's bladder pounded. He sat still when the Terry the giraffe cut his ropes and escaped into the dark night. He squirmed in his seat when Sherry the giraffe jump-kicked the hippo. And he had to leave when Perry the giraffe jumped from one roof to another without getting his neck caught on a power line. Brian ran through the dark hall of the movie theater, realizing that his mother had been right about one thing. He did have to leave the theater. It seemed like one of those situations people call ironic. But when Brian reached the movie theater bathroom, there was a line of ten men and even though Brian usually didn't have much foresight, he could tell there wasn't going to be enough time to wait. He rushed down another hall to another bathroom, but when he reached it, he realized why the other one was packed: this one was closed. Why would anyone close a bathroom? He wondered. He had no choice. He had to venture outside. He looked frantic as he rushed through the crowds of people buying their tickets and he almost fell down on the concrete as he hurried to the computer store next door. He burst through the door and looked around for the bathroom. "Can I help you?" said a confused man by the door. He had on a bright blue shirt and his face was covered in pimples. "Bathroom?" "I'm afraid we don't have one." The man said. Brian stomped his foot and left the store. Next door was a fabric store. He knew it was a store that only women went into; he had been there with his mother countless times but never his father. Did they even have a men's restroom? He had to chance it. He opened the door quickly and slid past it. An old woman with blue hair welcomed him. "Where is your mother, young man?" She asked. Brian shook his head. "Bathroom?" He was too pained to say much else. He was afraid his bladder couldn't hold it much longer. He danced in place waiting for the old woman to respond. "I can't help you unless you have your mother, young man. You're not old enough to be walking around stores alone." The old woman put her hands on her wide hips. Brian furrowed his eyes in frustration and as he left he poked his tongue out at her. Outside again and Brian couldn't find any other places to go to the bathroom. There was an empty field with two or three trees and cars stretching as far as the eye could see in the other direction. What was he going to do? And then he remembered his friend, Kyle, saying that on a camping trip, he had to go behind a tree. Could this be the solution to Brian's problem? Brian walked slowly toward the field. What if someone saw him? He would have to do it quickly. He nearly ran to the first tree in the field and looked around carefully. If someone saw him, who knew what would happen? He didn't see anyone. This was it. He had to go behind a tree across from the movie theater. There was nothing he could do about it. He unzipped his pants and instantly felt better. The pressure was gone and the world was a peace. He closed his eyes and savored the feeling. Then he felt an arm on his shoulder. "Just what are you doing young man?" Brian zipped up his pants and turned around, shaking. By the dark suit, the badge, and the thick dark mustache, Brian could tell it was a police officer. What had he done? What if he had broken the law? Was he going to get sent to jail, stuck behind bars? Was he going to have to sit in a cell for the rest of his life? No more video games, no more movies, no more T-ball? Brian whimpered, but couldn't say a word. "You know there are plenty of establishments that would let you use their restroom." The police officer said. Brian shook his head violently. "Did you try the one in the theater?" Now Brian got mad. "It was closed! And the other one had too long a line." The officer was taken aback. He stood back and rubbed his mustache. And then he smiled. "I could take you to jail, you know." "Please don't, sir! I promise I'll never go to the bathroom again!" The officer chuckled. "You don't have to do that, just not in public, okay?" Brian nodded, a tear slowly dropping down his pudgy cheek. The police officer walked to his bike and drove away. Brian walked back to the theater and stood outside the door where Super Ninja Giraffes was still playing. I can't believe Mom was right. What other things is she right about? He thought as he began opening the door. He heard clapping and then looked up as the credits began to flow down the big screen. He had missed the rest of the movie! He wondered what had happened to the ninja giraffes and if they ever found out where their sensei, the Diaphanous Elephant, was hidden. But instead of getting upset, he waited outside the door for his mother to come out. "How did you like the movie?" She asked. "It was good." Brian said. He decided not to tell her about his little adventure. It was too embarrassing. Instead, he ran to the door of the theater. "Brian, come back!" His mother called. But instead of coming back, he opened the door and politely stood in front of it for her. ©2007 Shelly Jasperson

20 years after we witnessed the comedy gold that was Harry Dunne and Lloyd Christmas, the duo has returned to usher in a whole new age of stupidity, and we have your first look at the new trailer. Starring the original duo of Jim Carrey and Jeff Daniels, and directed by the Farrelly brothers, Dumb and Dumber to is doing its very best to recapture the magic (and I'm sure the studio hopes financial success) of the first film. First impressions point to exactly that, as the first trailer really does feel like the original. Which needless to say, means that Harry and Lloyd are just as hilariously stupid as ever. Lets hope that after all these years dumb is still funny and that this sequel is worth the wait. Take a look at the trailer and let us know what you think? Is a little stupidity just what we need, or is this one sequel that is 18 years too late?

The present study was undertaken to compare serum and salivary biochemical constituents during lactation and dry period in dairy cows. Also, the present study evaluated for the first time the salivary biochemical constituents in dairy cows. The study was carried out using 45 healthy multiparous Holstein cows maintained in dairy farms located in Morioka city (Iwate prefecture, Japan). Cows were classified into groups based on the month of lactation. Serum, saliva and milk samples were collected and analyzed. Data were statistically analyzed and the variation in serum and salivary biochemical constituents during lactation and dry period were discussed. From the present study, it could be concluded that the 1st month of lactation has the highest levels for serum free fatty acids (FFA), β- Hydroxy butyric acid (BHBA) and aceto Acetic acid (ACAC). The dry period has the highest serum glucose level and the lowest serum FFA, BHBA and aspartate aminotransferase levels. Both serum and salivary FFA showed the highest value during the 1st month of lactation. Saliva contains a high level of gamma glutamyl transferase. The level of ammonia in saliva is higher than its serum level during all months of lactation and dry period. Most of the biochemical constituents in saliva change in different way from serum during lactation and dry period. Milk protein/fat ratio of 0.7 may be not indicative for subclinical ketosis. ABD ELLAH, Mahmoud R. et al. Comparison between Serum and Saliva Biochemical Constituents in Dairy Cows during Lactation and Dry Period. Journal of Advanced Veterinary Research, [S.l.], v. 5, n. 3, p. 143-150, july 2015. ISSN 2090-6277. Available at: <https://advetresearch.com/index.php/AVR/article/view/57>. Date accessed: 24 apr. 2019.

Eshratabad or Ashratabad () may refer to: Eshratabad Palace in Tehran Eshratabad, Isfahan Eshratabad, Kerman Eshratabad, Qazvin Eshratabad, Kashmar, Razavi Khorasan Province Eshratabad, Nishapur, Razavi Khorasan Province Eshratabad, Miyan Jolgeh, Nishapur County, Razavi Khorasan Province Eshratabad, Rashtkhvar, Razavi Khorasan Province Eshratabad, Sabzevar, Razavi Khorasan Province

Habitat Restoration & Land Management Sonoma Ecology Center's Restoration Department is dedicated to the restoration, stewardship and enhancement of over 4,000 acres of private and public lands around Sonoma Valley. Those include Sugarloaf Ridge State Park, Sonoma Garden Park, Sonoma Creek, Nathanson Creek, the Montini and Sonoma Overlook Trail Preserves, and Van Hoosear Wildflower Preserve. Throughout Sonoma Valley and beyond, our trained professionals provide ecological restoration services including: biological site assessments, native plant seed collection and propagation, invasive plant mapping and treatments, stream bank stabilization and erosion control, and fire fuel management. Our Restoration Team is working on public and private sites throughout Sonoma Valley and the surrounding regions of Northern California. To contact us about projects or to request our services, please email restoration@sonomaecologycenter.org or call 707-996-0712 ext. 116. Learn About Our Land Services Land Services Request Intake Form Our Restoration Department has more than 25 years of successful experience performing natural resource management projects to the satisfaction of public and private clients. We know how to plan for and accomplish management goals in sensitive habitats with effective, high-quality and affordable methods. Our depth of experience assures quality control on work we carry out, and allows us to incorporate adaptive management techniques that ensure project goals are met in a timely and efficient manner. We utilize smaller crews with technical expertise to maximize budgets and get projects done to a high level of quality. Our service area focuses on Sonoma Valley and extends to the surrounding regions of Northern California. We have experience working in many of northern California's natural habitats, including dunes, coastal scrub, grasslands, chaparral, oak woodlands, mixed evergreen forest, redwood forest, wetlands and riparian ecosystems. Our project team has the ecological knowledge, as well as specialized equipment and capacity, to carry out all of the requested services. Full service fish and wildlife habitat restoration for landowners (weed control, erosion control, bank stabilization, native plant installation, irrigation, maintenance) Native plant restoration Invasive non-native weed eradication: French broom, Himalayan blackberry, vinca, and English ivy. Native plant production – specializing in local/ watershed-sourced genotypes (seed collection/storage, propagation, growing/transplanting) Fish Barrier removal Stormwater management/low impact design (Erosion control, sediment filtration, etc.) Storm drain retrofitting (energy dissipater, detention basin, geotextile fabric installation). Bioengineering (willow revetments, willow mattresses, vegetated swales, etc) Erosion control (plants, geotextile, biodegradable fabric/ wattles, etc.) Land management (full service – Habitat restoration and management) Biochar Demonstrations and Biochar Kiln Burns Sonoma Creek Sonoma Creek is one of the most important streams in the Bay Area due to the habitat it provides for native fish and wildlife. For two decades, Sonoma Ecology Center's Restoration Department has serviced and improved Sonoma Creek by removing barriers to native fish migration, improving fish habitat, keeping stream banks and beds from washing away, slowing and filtering stormwater, invasive plant control, and planting native plants. We work constantly with creekside landowners and in partnership with Sonoma Water, the Wildlife Conservation Board, the California Coastal Conservancy and Environmental Science Associates to further the stewardship of Sonoma Creek. Montini Open Space Preserve and Sonoma Overlook Trail In collaboration with the City of Sonoma, Sonoma Ecology Center is responsible for the management and maintenance of both of these cherished open space preserves, located on the city's northern border. Our trained Restoration professionals conduct trail work, hazardous tree assessment, invasive plant control, and habitat restoration at both preserves, and help mitigate fire danger at Montini through invasive weed management, targeted mowing and by hiring grazing subcontractors. Restoration crew members also guide volunteers in planting, weeding and other volunteer events at the preserves. More on Montini / More on Overlook Working with the community, Sonoma Ecology Center designed and built this three-quarter-mile preserve with the support of $2 million in grant funding thanks to partnerships with the Sonoma County Agricultural and Open Space District, Sonoma Water, the U.S. Fish and Wildlife Service, and the City of Sonoma. This formerly neglected site now provides the community with clean recreational space, walking paths, signage, a Native Plant Demonstration Garden, a demonstration floodplain, and outdoor educational opportunities for three neighboring schools. It is also drawing native wildlife, including steelhead, back into its riparian habitat. Our Restoration staff continues to steward Nathanson Creek through management of invasive species, restoring habitats with native riparian plants, and managing stormwater debris and erosion control. More on Nathanson Our biologists and restoration ecologists have developed management plans for this 163-acre preserve, mapping natural resources as well as locations of sensitive and threatened species. As a result, threatened species are being protected while invasive species are managed and removed, helping return the preserve (which is protected under a conservation easement) to its original pristine state. Sonoma Ecology Center's Restoration Department is a key component of these ongoing conservation practices. More on Van Hoosear Native Plant Propagation Native plants are key to a healthy ecosystem: they support and sustain native fish and wildlife, stabilize creek banks, and—because they are adapted to native soils—they have more efficient water uptake and can improve water infiltration for groundwater. Over the last five years, SEC has grown and planted over 25,000 native plants. Read more about native plants and our Native Plant Nursery. Invasive Weed Removal After human development, invasive species are the second greatest cause of habitat loss in the world. In addition to displacing native plants and animals, invasives can increase fire risk and harbor pests. SEC staff created the model for eradication of Arundo (a highly invasive weed in Western streams) in California. The SEC Restoration Crew also specializes in the removal of French broom, Himalayan blackberry, vinca, and English ivy in addition to Arundo. Restoration Staff Barry Hill Senior Project Coordinator Barry graduated from UCSC with a degree in biology and later obtained an MS in watershed management from Humboldt State. He began his career in conservation as a wildland firefighter in Nevada, Oregon, and California. He was a hydrologist for the USGS and later for the U.S. Forest Service, and also worked as a geologist for the National Park Service. After retiring from federal service, Barry worked for county park departments in Santa Clara and Solano Counties, and for the California Land Stewardship Institute in Napa. He is a certified professional in erosion and sediment control. Restoration Project Manager ISA Certified Arborist ® WE-14183A Firefighter 2 Mr. Hernandez joined SEC in March 2022. He earned a BS in Biology from Sonoma State University while working for Sonoma State's Center for Environmental Inquiry as a trail steward and docent at Fairfield Osborn Preserve. He earned an M.S. in Biology from Sonoma State in 2020 studying the effects of sudden oak death on the fire severity of the 2017 Nuns Fire. Before coming to SEC he was a Part-time crew leader at Circuit Rider Community Services' Ecological Program. Kurtis McIntyre Restoration Technician Kurtis grew up in the Sonoma Valley. Being drawn to the outdoors, he found a job at Jack London State Historic Park where he realized an appreciation for the local ecology. Now he is dedicated to restoration work to help preserve the Sonoma Valley. Alex Kaste Alex Kaste received his undergraduate degree in Fisheries and Wildlife Conservation Science from Oregon State University and a certification in GIS. He has worked for non-profit organizations for the past 3 years, including as a habitat restoration technician with Greenbelt Land Trust in Corvallis, Oregon. Alex is particularly interested in fisheries conservation and had assisted in a graduate research project studying the effect of warming waters on Coastal Cutthroat populations in the Willamette River. He is an outdoorsman and is often found bird-watching or hiking at local parks and preserves. Zoe Hyland Zoe received her Associate's Degree in Natural Sciences from Santa Rosa Junior College and her B.A. in Communications from UC Davis. For the past three years, she worked at Green String Farm learning about sustainable farming practices and soil health. She developed a love for restoration and conservation after two semesters as an intern in the Santa Rosa Junior College Fire Resiliency Program. Her goal is to continue learning more about restoration in the Sonoma Valley and develop her skills in prescribed fire. CA Delta Conservancy CA Dept of Fish and Wildlife CA Dept of Water Resources CA Natural Resources Agency Center for Ecosystem Management & Restoration ESA PWA Prunuske Chatham, Inc. San Francisco Estuary Project Solano Resource Conservation District Sonoma County Water Agency Sonoma Resource Conservation District US Environmental Protection Agency

Miravel 1952 is a big red wine gem from the Stellenbosch wine region of South Africa. Miravel is a handcrafted family blend and a name that honors family footprints from many different lands. Maarten van Beuningen, until a few years ago, was a Zimbabwean farmer farming fruit, cattle, pigs and ostriches for much of his life; now he and his family find themselves in Somerset West, embarking on a new career as wine farmers. Maarten and his wife Janine acquired a portion of Dellrust, and have built a home on their new property, with a view to turning a lifelong love of wine into a new way of life. Miravel 1952 is a big ruby-colored red made of 49% Cabernet Sauvignon, 41% Merlot, and 10% Petit Verdot. The wine shows beautiful dark berry fruit with lovely hints of chocolate, spice and licorice. Matured in old French oak for 24 months, it is full-bodied, complex and well balanced to age for 8-10 years. On the finish, it is long and velvety. Only 776 bottles produced. Enjoy Miravel 1952 with filet mignon, beef Bourguignon, roasted duck breast and aged cheeses.

Get a Genuine Brannock device for online shoe shopping. Foot Health and Kids Growth European Childrens Shoes Blog Shoes in Ads and Art Blog Brannock History Scroll down or shop by type of Brannock device + - Junior (US) Adult (US) Euro/ UK/ Centimeter Accessories/Extras Instructions/ Shoe Fitting Scroll down or shop by type of Brannock device The Brannock Device® is the standard foot measuring tool for the world's footwear industry. But few people are able to call the device by name, much less identify its inventor, Charles Brannock. Brannock was born into the shoe business. His father, Otis Brannock, joined with Ernest Parks in 1906 to found the downtown Park-Brannock Shoe Co. in Syracuse, New York. As a Syracuse University student, young Brannock wanted to find the best way to measure the foot. He played around with the idea for a couple of years and finally built a prototype using an Erector set. In 1926 and 1927, Brannock patented the device and created a company to build it. Before the Brannock Device®, the available option was a primitive block of measured wood. The Brannock Device® dramatically improved the accuracy of a foot measurement, to 95-96 percent right. The size system is linear. For example, a Men's size 1 is 7-2/3 inches. Each additional size is 1/3 inch longer. Widths work the same way. Each width is separated by a distance of 3/16 of an inch. There are actually nine widths in the US system (width actually varies with foot length): AAA, AA, A, B, C, D, E, EE, and EEE. The Brannock Device® comes in green, purple, red or black. There are models for men, women, athletic shoes and ski boots, and for children, always with two knobs for adjusting the fit cups at both ends for the curve of the heel, and a sliding bar for adjusting "firmly for thin foot, lightly for wide foot." A Unique Device At first, the invention was valued for what it did for the local shoe store. No one else in Syracuse could fit a shoe so perfectly. If someone had an unusual size, and the device picked it up, Brannock made sure he had a match in stock. Soon, however, word of the device got around, and demand was suddenly booming. In fact, during World War II, the Army hired Brannock to ensure that boots and shoes fit enlisted men. That's when Brannock first expanded his manufacturing facilities. Brannock believed in all the things that are supposedly dead in industry. He loved small business. He loved working downtown. And he built his product to last. While some had advised Brannock to make his devices out of plastic, ensuring that they would need to be replaced every couple of years, he refused to entertain that notion, and would only make them from durable steel. Today, most shoe stores don't get rid of their Brannock Devices for 10 or 15 years, until the numbers finally wear away from so much use. Throughout the 1980s, Brannock showed up in the office every working day to take care of business. His health began to fail then, and he considered selling the business, but any would-be buyer had to guarantee the device would not be cheapened or changed. That point was not negotiable. He died in 1993 at the age of 89. The company was purchased by Sal Leonardi during that decade. Today, the Brannock Device® remains the standard for the footwear industry. With more than one million devices sold, the Brannock Device® has varied very little over the years. However, the company, under its new owners, has started manufacturing customized models and is currently considering producing a digital model. web@brannockdevices.com Copyright © 2022, BrannockDevices. Powered by Shopify

We are looking for a graphic designer/art worker for a few weeks to help establishing a new brand to existing printed documents and other branded materials that could also include some digital asset creation as well. The ideal candidate can work comfortably in Adobe InDesign, Photoshop and Sketch. Please send your application with CV/ portfolio/ rates/ availability to recruiter Anna Persson at anna@nucreatives.com. Mark it 'GD London'.

Med Review Renews, Expands Office Lease at 199 Water Street in New York City Posted on April 24, 2019 by Taylor Williams in Leasing Activity, New York, Northeast, Office 199 Water Street in New York City spans 1.1 million square feet across 35 stories. NEW YORK CITY — Med Review, a subsidiary of the New York County Health Services Organizations that provides healthcare assessment services, has inked a deal to lease 72,439 square feet of office space at 199 Water Street in New York City. As part of the deal, the firm is taking an additional 35,454 square feet on the seventh floor, while also renewing its 36,985-square-foot space that spans the entire 27th floor. Built in 1984, 199 Water Street was renovated in 2014 to offer upgraded signage and lighting, as well as to add a new parking garage and tenant amenities. Brett Greenberg and Adam Rappaport represented the landlord, Jack Resnick & Sons, on an internal basis, along with John Cefaly, Robert Constable, Ethan Silverstein, Stephen Bellwood and Myles Fennon of Cushman & Wakefield. Mark Ravesloot and Bill Iacovelli of CBRE represented Med Review. Other tenants at the 1.1 million-square-foot property include The Howard Hughes Corp. and Allied World Insurance Co. Previous Previous post: Trammell Crow Co. to Open New Office in Boston Next Next post: Greystar Acquires 266-Unit Multifamily Property in Manhattan Knotel to Open 12,500 SF Flexible Office Space in Lower Manhattan ABS Partners Negotiates 9,903 SF Office Lease in The Bronx Flatiron Health Signs 252,452 SF Office Lease Renewal, Expansion in Manhattan Greenhill Signs 78,000 SF Office Lease at Manhattan's Rockefeller Center Consulate General of Japan Signs 58,000 SF Lease Extension at 299 Park Avenue in Manhattan Cold Storage Yet to Tap Full Potential Retail Landscape Evolves in Fairfield, Westchester Counties Occupancy, Rental Rates Are on the Rise in Detroit's CBD Office Market Will Your Project Get Built? Working With Communities to Gain Support for Development Charming Charlie Declares Second Bankruptcy, Will Close All 261 Stores Twin River Worldwide Agrees to Acquire Two Casinos from Eldorado Resorts for $230M Occidental Management Closes on Acquisition of 190-Acre Sprint Headquarters in Overland Park, Kansas

Thorough knowledge of the processing and flow of mail. Knowledge of postal regulations that relate to bar coded mailings. Ability to lift up to 50 lbs. with or without accommodations. Ability to walk, stand, stoop, and bend 80% of the time with or without accommodation. Candidates must have a basic enough understanding of English to be able to understand and follow safety and other operational directions communicated in English, irrespective of their native language. Must have the ability to work overtime which may include weekends, holidays and or a different shift with little or no advance notice. Ensures that the mail is reviewed for proper date, postage, and good quality barcodes prior to being placed into production. Prepares outgoing mail and conducts first quality check on completed production run. Organizes the mail flow on the production floor by assigning workers and assessing volumes and type of mail based on current staffing levels to meet postal deadlines. Completes end of run production tasks (i.e. proper disposition of extra mail, placement of bins, stocking trays and tag holders, stocking all supplies needed to run production, etc.). Responsible for overseeing the Departmental Supervisors on designated shifts. Operation Supervisor responsibilities include interviewing, hiring, training, appraising performance, rewarding, disciplining, and addressing complaints/solving problems. Oversees quality function throughout the Operations floor. Assures quality processes and procedures are followed at all times. Completes required USPS and internal reports, job costing reports and application of all USPS postal regulations. Assures clear and concise communication of pertinent information between shifts. Offers suggestions regarding increasing efficiency of equipment use, working conditions, and motivation of employees. Promotes a clean work area to prevent possible injuries and/or tripping hazards to self and others. On-Demand Mail Services (ODMS) offers Domestic and International postal optimization solutions to our customers nationwide. We do this as part of the United States Postal Service's Work Share Program, a program that provides mailers and presort bureaus with significant discounts for preparing mail according to strict USPS specifications. Our Customers depend on us as their Mailing Experts. How you perform in the position will determine how successful we are. Our Mission is Customer Service and Quality, Quality, Quality! Your journey as an ODMS Associate, will begin with on the job QUALITY training along with periodic observed and written qualification assessments. It is vital that you learn everything you are taught, show up for work on time each day and continue to perform your duties to the highest level possible.

english-quiz.net Toto SDY Posted byadminpro January 17, 2023 January 17, 2023 The Singapore Lottery is one of the most popular forms of gambling in Singapore. A recent survey conducted by the National Council on Problem Gambling revealed that 44% of the country's permanent residents had placed bets on gambling activities in the past year. It's important to understand the risks of playing the Singapore Lottery before you buy your tickets. Even with a relatively low odds, winning can be quite a big prize. In Singapore, Toto, also known as Singapore Lotto, is the most popular betting game. Players choose six numbers from 1 to 49 and have a chance to win a jackpot. This game is played twice a week. Toto was launched in Singapore in 1968. After years of evolution, it became the 6/42 format in 1988. In 2014, the prize was increased to SGD$1 million. Despite the low odds of winning, lottery tickets are a great way to enjoy the thrill of the live draw. Moreover, tickets can be purchased online for convenience. Singapore Pools, the only legal provider of lottery games in the country, also offers a variety of other games. Some of these include Sweep and 4-D, which are played on a weekly basis. The Singapore Pools branch conducts 4-D draws on Sundays. These drawings are overseen by the Tote Board. While the Tote Board is a statutory body, the company that operates the lottery is a private one. If you are caught placing bets with an unlicensed or illegal operator, you could be fined up to SGD10,000. Posted byadminpro January 17, 2023 January 17, 2023 Posted inUncategorized Lottery Singapore How to Choose a Reputable Lottery Online Site What to Look for in a Slot Keluaran Sidney english-quiz.net, Proudly powered by WordPress.

Chapter 4 Ongoing Projects and the Future Menu of Topics Author: International Monetary Fund. Independent Evaluation Office IEOR; IEO; InWent seminar; The IMF and Aid to Sub-Saharan Africa; evaluation; IMF trade policy advice; IMF research; IMF accountability; IMF position; IMF interaction; Sub-Saharan Africa The IEO is currently engaged in the preparation of two evaluation projects: "The IMF's Interactions with Its Member Countries" and "The IMF's Approach to International Trade Policy Issues." One other evaluation, "The IMF's Research Agenda," is expected to commence in FY2009. Table 4.1 shows the status of the various evaluations completed, in progress, or in the work program of the IEO. The IEO has now begun a transparent consultation process with the IMF's Executive Board, Management, and staff, as well as external stakeholders to identify a set of topics to be added to the office's pipeline in FY2009. This chapter details those evaluations currently in progress. Table 4.1. Completed and Ongoing IEO Work Program 1 The date refers to the time the completed report was, or is expected to be, circulated to the Evaluation Committee of the Executive Board. 2 Refers to the fiscal year in which the projects were first added to the work program. Initial round of evaluation projects Prolonged Use of IMF Resources Completed (August 2002) The IMF and Recent Capital Account Crises (Indonesia, Korea, Brazil) Completed (May 2003) Fiscal Adjustment in IMF-Supported Programs Completed (July 2003) Additions to work program2 The IMF and Argentina, 1991–2001 Completed (July 2004) Poverty Reduction Strategy Papers/Poverty Reduction Growth Facility Completed (June 2004) IMF Technical Assistance Completed (January 2004) The IMF's Approach to Capital Account Liberalization Completed (April 2005) IMF Assistance to Jordan Completed (October 2005) Financial Sector Assessment Program Completed (November 2005) Multilateral Surveillance Completed (March 2006) Structural Conditionality in IMF-Supported Programs Completed (October 2007) The IMF and Aid to Sub-Saharan Africa Completed (January 2007) IMF Exchange Rate Policy Advice Completed (March 2007) Governance of the IMF Completed (April 2008) The IMF's Interactions with Its Member countries In progress The IMF's Approach to International Trade Policy Issues In progress The IMF's Research Agenda To commence in FY2009 The IMF's Interactions with Its Member Countries To achieve its objectives, the IMF depends in large part on having effective interactions with member countries. Interactions, in this context, are defined to include exchanges of information, analysis, and views between IMF officials and country authorities, or other people or entities in member countries. The evaluation will examine whether the interactions between the IMF and its member countries, carried out in different circumstances and for various objectives, have been effective and well managed. It will cover the period 2001 to early 2008. There are motivations for evaluating the IMF's interactions with member countries at this time, besides their inherent importance to the institution's effectiveness. First, evidence from a variety of sources—including country authorities and civil society—suggests that these interactions could be improved. Both the importance of the interactions and their shortcomings have been recurrent themes in previous evaluations by the IEO, as well as in comments by country authorities, civil society, and academics. Frustrations have also been expressed by IMF staff. Second, the nature of IMF interactions with member countries has evolved in recent years, reflecting changes in policies and priorities, and more changes are in prospect; the ways in which change has been managed is itself an important topic. The evaluation questions respond to issues raised about the effectiveness of interactions by country authorities and others. Authorities' views on what has been successful and unsuccessful were sought in confidential interviews. In addition, in examining various criticisms of the interactions and determining the extent of their validity, the evaluation will take account of concerns that have been made public, as well as the changes in Fund policies that have taken place in recent years. The success or otherwise of the new approaches and priorities, some of which have been adopted in response to previous criticisms, is an important aspect of the evaluation. The evaluation will also examine how the IMF's interactions have been managed. A key element is to determine how adaptations are made to changing priorities, changing country circumstances, and feedback from partners, especially the country authorities. In this respect, the way in which other institutions have managed country or "client" relationships could be relevant and instructive, including the balance between management from head office and by local presence. The effectiveness of interactions will be evaluated against various criteria. These include criteria based on the guidance provided to IMF staff, issues considered important by country authorities, and additional criteria that may be suggested by referring to policies and practices in other institutions. Two aspects will be examined in particular—the clarity of the purpose and scope of interactions, and the quality of the interactions. The evaluation will assess the IMF's management of interactions against two metrics: (1) the Fund's own policies for managing interactions; and (2) the policies and practices of other institutions, and some models of what best practice might be in this area. The evaluation will be based on evidence provided by seven main elements: a description of the mandate, policies, and guidance governing the IMF's interactions with member countries; an account of the IMF's process for managing the interactions with its members; a description, using data from IMF internal sources, of how the interactions with member countries evolved over the evaluation` period; a review of other institutions' policies and practices for conducting interactions; opinion surveys of country authorities, civil society, and IMF staff and Executive Directors; and a study of interactions in about 40–50 countries, involving interviews with staff and country authorities; and thematic studies of interactions in a few countries. The evidence, triangulated from various perspectives, will be distilled into a short overview report containing the main findings and recommendations. The IMF's Approach to International Trade Policy Issues The IMF's involvement in trade policy issues for well over 25 years has been a source of controversy. It is widely accepted that Article I(ii) of the IMF's Articles of Agreement, imbuing the IMF with the responsibility "to facilitate the expansion and balanced growth of international trade," provides the mandate for its involvement in issues affecting international trade. The mandate is reinforced by references in Article IV to members' obligations on economic policies in collaborating with the Fund to assure orderly exchange arrangements and in Article V to the IMF's role in assisting members in solving balance of payments problems. But in contrast to exchange rate arrangements and the system of exchange rates, international trade is not under the regulatory jurisdiction of the IMF. Thus, the mandate is less definitive on the specific nature of the IMF's involvement in trade issues than on its role in exchange rate policy. The relatively soft mandate for IMF involvement in trade policy issues leaves substantial scope for disagreement on whether the IMF has overstepped its proper role on trade policy or not done enough. Moreover, reflecting a fundamental orientation toward furthering free trade, the IMF's involvement in trade policy issues has at times stoked the debate on whether steps toward freer trade are always beneficial for the country concerned or if developmental objectives (particularly in low-income countries) would be better served by programs more tailored to country-specific circumstances. Alongside this debate are charges that IMF advice is not even-handed and pushes harder on developing countries (typically the borrowers from the IMF) than on advanced countries to reduce protectionism. In recent years, with the increasing complexity of trade policy issues, questions about whether IMF staff have the capacity to address trade policies sufficiently systematically have also arisen. The evaluation will focus on the clarity and conduct of the IMF's role in trade policy advice and advocacy. It will aim to move beyond the question of whether the IMF's role should be more focused and address questions of whether interpretation of the mandate for involvement in trade policy is well based and clear; whether critics of IMF positions have a solid basis and where the IMF's comparative advantage in trade policy issues lies; how well the IMF has adapted to the establishment of the World Trade Organization (WTO); and whether the IMF's trade policy advice is effective. In its conclusions, the evaluation will consider possible improvements in how the IMF defines its role and executes trade policy advice. The evaluation will assess the IMF's work on trade within the standard results-chain framework of inputs, outputs, and outcomes. Inputs consist of IMF internal processes such as the guidance provided for trade work by the Board and Management, and research and analysis—from inside and outside the IMF—that focus and challenge the IMF's advice on trade policy. Outputs are defined as assessments, analyses, and communication, in surveillance reports, program documents, technical assistance reports, policy dialogues, communiqués, speeches, and statements, among others. Both inputs and outputs are, to a large extent, within the control of the IMF; thus, IMF accountability for their quality is high. Moving along the results chain, the degree of IMF accountability for results becomes increasingly affected by other factors. Outcomes are defined as country authorities' reception of IMF trade policy advice and changes in trade policies and the trade environment in general. The evaluation will cover the period since the establishment of the WTO—1996–2007—but may go back further for some questions and focus on the more recent past for others. The main instruments of the evaluation will be desk reviews of policy documents and guidelines issued to staff, Article IV and program documents, Executive Board minutes, and advocacy and outreach items. There will also be case studies of the IMF's involvement with individual countries (or country groupings) and/or trade policy issues that cut across countries (or country groupings). These will be supplemented by surveys and interviews of current and former IMF, WTO, and World Bank staff as well as government and nongovernment representatives (including the media). The IMF's Research Agenda Seven years ago, a group of independent experts evaluated the IMF's economic research activities. At that time, the Executive Board agreed with the group's finding that there was "substantial room for improvement in the overall quality of the IMF's research." Among other conclusions, Directors endorsed the recommendation that the mix of research conducted at the IMF would need to be directed more to areas where it could add the most value and agreed that it could be integrated to a greater extent into policy work—an assessment that has also been shared by external critics of the IMF. The evaluation would be a follow up exercise and look at two areas. First, the evaluation would examine the way in which research topics are selected and priorities imposed across the IMF, and the extent to which the recommendation has been carried out to direct research more to areas where it could add the most value and be better integrated into policy work. This will focus both on the use of research for technical assistance purposes, and also in aspects of policy review, dialogue and in recommendations made to member countries. In order to do this, an analysis of the research conducted by all departments in the IMF would be undertaken, using as a starting point the work performed by the existing interdepartmental Research Committee. The evaluation would attempt to identify which pieces of research had been particularly relevant and influential for the country and policy work of the IMF. In addition, a survey of staff and country officials would try to elicit whether some topics could have received greater priority. Given the attempt to streamline and focus IMF activities in recent years, and to seek ways to save costs—issues that will be of even greater importance in the years to come—the evaluation would explore the extent to which decisions on research topics are guided by the opportunities to rely on research conducted outside the IMF, either at other institutions such as the World Bank and the Organization for Economic Cooperation and Development, or in academia. Second, the evaluation will investigate aspects of the quality (as opposed to the scope and relevance) of IMF research. The process by which IMF research is supervised and vetted would be examined. The views on a sample of research would be sought from a panel of external experts. Issues such as the degree of innovation and the consistency with first best methodology would be studied. Within Same Series CHAPTER 4 Ongoing Topics and Future Menu of Evaluation Topics Chapter 3. Ongoing Topics and Future Menu of Evaluation Topics Chapter 4. Looking Ahead: Ongoing Evaluations and the IEO Work Program CHAPTER 3 Status of Ongoing Projects 4 Ten Years of IMF Trade-Related Work: An Assessment CHAPTER 2 Recently Completed Evaluations and Follow-Up on Past Evaluations CHAPTER 1 The Independent Evaluation Office: Objectives and Modes of Operation Chapter 4: Ongoing Topics and Future Menu of Evaluation Topics Chapter 4 Looking Ahead: The IEO Work Program and Future Menu of Topics Chapter 8 IEO Retrospective: Ten Years of Independent Evaluation at the IMF Chapter 4: Ongoing Evaluations CHAPTER 7 Transparency, Accountability, and Cooperation External Evaluation of the Independent Evaluation Office: Report of the Panel Convened by the IMF Executive BoardExternal Evaluation of the Independent Evaluation Office: Report of the Panel Convened by the IMF Executive Board Part II: Studies of Independent Evaluation at the IMF Other Publishers Content Disaster Risk Assessment for Project Preparation: A Practical Guide Together We Deliver: Grants for a Brighter Future Research Futures: Projecting Agricultural RandD Potentials for Latin America and the Caribbean Civil Society and Trade Diplomacy in the "Global Age": The European Case; Trade Policy Dialogue between Civil Society and the European Commission Civil Society and Trade Diplomacy in the "Global Era": Dialogue on Trade Policy between Civil Society and the European Commission Aid for Trade and the Mesoamerica Project Evaluation of MIF Projects: Environment Trading Promises for Results: What Global Integration Can Do for Latin America and the Caribbean; Chapter 6 Haiti: Changements climatiques; Donnees historiques et projections futures Taking Stock of IDB Lab's Skills for the Future Projects 2016-2019 Evaluation of the MIF Projects: Microfinance Is Gender Being Mainstreamed in Bank's Projects? International project marketing (ICM 6) A just transition for all: Can the past inform the future?: International Journal of Labour Research, IJLR, Vol 6, No 2/2014 Financing international projects (ICM 3) Nordic Council of Ministers Saltsjobaden V - Taking international air pollution policies into the future: Gothenburg 24-26 June 2013 Biological Waste Treatment in Norway and Sweden: What works well and what can be improved?: A synthesis of the nine projects in BUS Existing Default Values and Recommendations for Exposure Assessment: A Nordic Exposure Group Project 2011 EU REACH Enforcement project: Final report 2008 Aid for Trade: Building On Progress Today for Tomorrow's Future China, India, and the Future of the World Economy: Fierce Competition Or Shared Growth? What does regional trade in South Asia reveal about future trade integration?: Some empirical evidence / Future Armenia: Connect, Compete, Prosper. Work for a Better Future in Armenia: An Analysis of Jobs Dynamics Lessons from Land Administration Projects: A Review of Project Performance Assessments Integrating Social Accountability Approaches into Extractive Industries Projects: A Guidance Note Belize, Right Choices Bright Future: Systematic Country Diagnostic. Georgia Public Expenditure Review: Building a Sustainable Future. Future inequality in CO2 emissions and the projected impact of abatement proposals https://www.elibrary.imf.org/view/books/017/09537-9781589067790-en/ch04.xml IEO Annual Report, 2008 Chapter 1 Overview of Developments in Financial Year 2008 Chapter 2 Recently Completed Evaluations Chapter 3 Cross-Cutting Themes Appendix 1 Administrative Budget: Independent Evaluation Office Appendix 2 Outreach Activities in FY2008

Limit my search to Chromosomes and Gene Expression Chromosomes and Gene Expression Viruses: A frameshift in time The efficiency with which ribosomes shift reading frames when decoding viral RNA may change over the course of an infection. Martina M Yordanova Pavel V Baranov Martina M Yordanova, Pavel V Baranov , School of Biochemistry and Cell Biology, University College Cork, Ireland; When an RNA virus infects a cell, ribosomes inside the cell decode the genetic information in the virus's RNA to produce proteins, which are then used to make more viral particles. A single-stranded RNA molecule consists of a sequence of nucleotides that the ribosome reads three at a time. Each triplet, or codon, codes for either an amino acid (the building blocks that form proteins), or signals for the ribosome to start or stop reading the RNA sequence. Therefore, each nucleotide sequence can therefore be 'read' by ribosomes in three different ways, or 'reading frames', depending on which nucleotide the ribosome starts reading from. Additionally, an 'open reading frame' or ORF is a sequence of nucleotide triplets that code for amino acids located between two stop codons in the same reading frame. Almost all cellular proteins are encoded in a single reading frame, with only rare exceptions (Baranov et al., 2015). Viruses, however, often break this rule in a process termed 'programmed ribosomal frameshifting' (Firth and Brierley, 2012; Atkins et al., 2016). This mechanism occurs at specific locations in the nucleotide sequence called frameshift sites, where a proportion of the ribosomes translating the RNA will shift back or forward one nucleotide and start decoding a different reading frame. Meanwhile, the rest of the ribosomes continue reading the original frame. Thus, the same segment of an RNA molecule can be read to produce two protein molecules with distinct amino acid sequences simultaneously. It is unclear exactly why viruses employ programmed ribosomal frameshifting. One suggestion is that this mechanism allows for a more compact organization of genetic material. Another is that frameshifting could be used for setting a specific ratio between different viral proteins. Most commonly, ribosomal frameshifting occurs during the synthesis of viral polyproteins, long amino acid chains that are processed into smaller proteins with distinct functions. The advantage of organizing protein synthesis in this way is that only one RNA molecule is needed to encode multiple proteins. However, if all these proteins were synthesized as a part of a single polyprotein, they would occur strictly in a one-to-one ratio after being processed. This would be wasteful, since these proteins are needed in different quantities. So how could the optimal proportions of these proteins be achieved? The low efficiency frameshifting mechanism solves the problem. Proteins that the virus needs in large quantities are encoded early in the sequence in an open reading frame herein referred to as ORF1A, while proteins that the virus requires in lower quantities are encoded in a different but overlapping downstream reading frame, herein referred to as ORF1B (Figure 1). ORF1A is decoded according to standard rules, producing a shorter version of the viral polyprotein. ORF1B, on the other hand, is only read by the ribosomes that change reading frame at the frameshift site between ORF1A and ORF1B, resulting in a longer polyprotein. Schematic representation showing how RNA is decoded in the vicinity of the frameshift site between two open reading frames, ORF1A and ORF1B. Top: most ribosomes (yellow) decoding ORF1A terminate at the stop codon (red arrow), release the protein (not shown) and dissociate from the RNA (gray curve). A small proportion of ribosomes, however, shift frames to decode ORF1B. The ribosome at the frameshift site is outlined with a fuzzy cloud. Center: the density of ribosome footprints (the lines under each of the ribosomes) revealed by ribosome profiling maps to the positions occupied by ribosomes on the RNA molecule. The ratio between the ribosome footprint density at ORF1A and at ORF1B can be used as a measure of frameshifting efficiency. Bottom: schematic of the three possible reading frames in a molecule of RNA, each represented by a bar and denoted by –1, 0, and +1. The clock-like nature of the frameshift site drawing alludes to the temporal regulation of frameshifting as revealed by Cook et al. This type of frameshifting is sometimes referred to as canonical due to its common occurrence in RNA viruses. It was originally assumed that the ratio of products generated from ORF1A and ORF1B was fixed throughout the virus's time in the cell (Jacks and Varmus, 1985). Now, in eLife, Ian Brierley, Andrew Firth, Ying Fang and colleagues – including Georgia Cook (University of Cambridge) as first author – report evidence suggesting that this ratio changes over the course of infection (Cook et al., 2022). The team (who are based at various institutes in the United Kingdom, the United States and China) studied how viral gene expression changes during porcine reproductive and respiratory syndrome virus (PRRSV) infection. To do this, Cook et al. used a technique called ribosome profiling to map which parts of the virus's RNA sequence were being translated by ribosomes at any given time (Ingolia et al., 2009). These mappings, called ribosome footprints, revealed several new ORFs that encoded previously uncharacterized viral protein products. Ribosome profiling can also be used to compare how efficiently different proteins are synthesized. For example, in the PRRSV genome the density of footprints mapped to ORF1A is higher than the footprint density at ORF1B. This happens because only a small proportion of the ribosomes reading ORF1A shift reading frame and proceed to ORF1B (Figure 1). By calculating the ratio of footprint densities between the two open reading frames it is possible to estimate frameshifting efficiency. The PRRSV genome is known to contain two frameshift sites: the canonical site between ORF1A and ORF1B, which is used by many viruses, and a second, rarer frameshift site in ORF1A that results in the production of a shorter polyprotein. The genome of a related virus, called the encephalomyocarditis virus, has been shown to have a similar secondary frameshift site that is stimulated by a viral protein (Napthine et al., 2017). The concentration of this viral protein was found to increase over the course of an infection and cause more ribosomes to shift to the other reading frame. However, by measuring the efficiency of both frameshifting sites in PRRSV, Cook et al. showed that this temporal change is not limited to the protein-stimulated frameshifting, but also occurs in the canonical site between ORF1A and ORF1B. This finding challenges the current paradigm that regards the canonical frameshifting between ORF1A and ORF1B as a mechanism that enables a fixed ratio between polyprotein products. The temporal change detected in PRRSV suggests that the efficiency of frameshifting may also be altered in other viruses over time. If so, it would be interesting to determine what factors mediate the regulation of the frameshifting event between ORF1A and ORF1B. An open question that remains is how changes in frameshifting efficiency along the course of an infection relate to the virus's virulence and transmissibility. It is possible that changes in efficiency are simply due to alterations in the infected cell that make ribosomes more prone to shifting to another reading frame. However, it may be that regulating the efficiency of frameshifting is beneficial for the virus. Alternatively, the antiviral response of the host may induce frameshifting to alter the ratio of viral proteins and negatively impact the virus. Indeed, it has been previously reported that the formation of viral particles can be disrupted by altering frameshifting efficiency (Dulude et al., 2006). Whatever the case, the search for cellular factors responsible for changes in frameshifting has already begun (Riegger and Caliskan, 2022). The identification of these factors will provide researchers with new targets for modulating frameshifting efficiency in viruses, potentially revealing new ways to fight off viral infections. Atkins JF Loughran G Bhatt PR Firth AE Baranov PV (2016) Ribosomal frameshifting and transcriptional slippage: from genetic steganography and cryptography to adventitious use Nucleic Acids Research 44:7007–7078. https://doi.org/10.1093/nar/gkw530 Yordanova MM (2015) Augmented genetic decoding: global, local and temporal alterations of decoding processes and codon meaning Nature Reviews Genetics 16:517–529. Cook GM Brown K Shang P Soday L Dinan AM Tumescheit C Mockett APA Fang Y Brierley I (2022) Ribosome profiling of porcine reproductive and respiratory syndrome virus reveals novel features of viral gene expression Dulude D Berchiche YA Gendron K Brakier-Gingras L Heveker N (2006) Decreasing the frameshift efficiency translates into an equivalent reduction of the replication of the human immunodeficiency virus type 1 Virology 345:127–136. https://doi.org/10.1016/j.virol.2005.08.048 (2012) Non-canonical translation in RNA viruses The Journal of General Virology 93:1385–1409. https://doi.org/10.1099/vir.0.042499-0 Ingolia NT Ghaemmaghami S Newman JRS Weissman JS (2009) Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling Jacks T Varmus HE (1985) Expression of the Rous sarcoma virus pol gene by ribosomal frameshifting Napthine S Ling R Finch LK Jones JD Bell S (2017) Protein-directed ribosomal frameshifting temporally regulates gene expression Riegger RJ Caliskan N (2022) Thinking outside the frame: impacting genomes capacity by programmed ribosomal frameshifting Frontiers in Molecular Biosciences 9:842261. https://doi.org/10.3389/fmolb.2022.842261 Martina M Yordanova is in the School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland Pavel V Baranov is in the School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland p.baranov@ucc.ie Version of Record published: April 11, 2022 (version 1) © 2022, Yordanova and Baranov arterivirus porcine reproductive respiratory syndrome virus PRRSV ribosome profiling programmed ribosomal frameshifting subgenomic mRNAs Ribosome profiling of porcine reproductive and respiratory syndrome virus reveals novel features of viral gene expression Georgia M Cook, Katherine Brown ... Ian Brierley Research Article Updated Apr 11, 2022 The arterivirus porcine reproductive and respiratory syndrome virus (PRRSV) causes significant economic losses to the swine industry worldwide. Here we apply ribosome profiling (RiboSeq) and parallel RNA sequencing (RNASeq) to characterise the transcriptome and translatome of both species of PRRSV and to analyse the host response to infection. We calculated programmed ribosomal frameshift (PRF) efficiency at both sites on the viral genome. This revealed the nsp2 PRF site as the second known example where temporally regulated frameshifting occurs, with increasing −2 PRF efficiency likely facilitated by accumulation of the PRF-stimulatory viral protein, nsp1β. Surprisingly, we find that PRF efficiency at the canonical ORF1ab frameshift site also increases over time, in contradiction of the common assumption that RNA structure-directed frameshift sites operate at a fixed efficiency. This has potential implications for the numerous other viruses with canonical PRF sites. Furthermore, we discovered several highly translated additional viral ORFs, the translation of which may be facilitated by multiple novel viral transcripts. For example, we found a highly expressed 125-codon ORF overlapping nsp12, which is likely translated from novel subgenomic RNA transcripts that overlap the 3′ end of ORF1b. Similar transcripts were discovered for both PRRSV-1 and PRRSV-2, suggesting a potential conserved mechanism for temporally regulating expression of the 3′-proximal region of ORF1b. We also identified a highly translated, short upstream ORF in the 5′ UTR, the presence of which is highly conserved amongst PRRSV-2 isolates. These findings reveal hidden complexity in the gene expression programmes of these important nidoviruses. Vernalization-triggered expression of the antisense transcript COOLAIR is mediated by CBF genes Myeongjune Jeon, Goowon Jeong ... Ilha Lee To synchronize flowering time with spring, many plants undergo vernalization, a floral-promotion process triggered by exposure to long-term winter cold. In Arabidopsis thaliana, this is achieved through cold-mediated epigenetic silencing of the floral repressor, FLOWERING LOCUS C (FLC). COOLAIR, a cold-induced antisense RNA transcribed from the FLC locus, has been proposed to facilitate FLC silencing. Here, we show that C-repeat (CRT)/dehydration-responsive elements (DREs) at the 3′-end of FLC and CRT/DRE-binding factors (CBFs) are required for cold-mediated expression of COOLAIR. CBFs bind to CRT/DREs at the 3′-end of FLC, both in vitro and in vivo, and CBF levels increase gradually during vernalization. Cold-induced COOLAIR expression is severely impaired in cbfs mutants in which all CBF genes are knocked-out. Conversely, CBF-overexpressing plants show increased COOLAIR levels even at warm temperatures. We show that COOLAIR is induced by CBFs during early stages of vernalization but COOLAIR levels decrease in later phases as FLC chromatin transitions to an inactive state to which CBFs can no longer bind. We also demonstrate that cbfs and FLCΔCOOLAIR mutants exhibit a normal vernalization response despite their inability to activate COOLAIR expression during cold, revealing that COOLAIR is not required for the vernalization process. An atrial fibrillation-associated regulatory region modulates cardiac Tbx5 levels and arrhythmia susceptibility Fernanda M Bosada, Karel van Duijvenboden ... Vincent M Christoffels Heart development and rhythm control are highly Tbx5 dosage-sensitive. TBX5 haploinsufficiency causes congenital conduction disorders, whereas increased expression levels of TBX5 in human heart samples has been associated with atrial fibrillation (AF). We deleted the conserved mouse orthologues of two independent AF-associated genomic regions in the Tbx5 locus, one intronic (RE(int)) and one downstream (RE(down)) of Tbx5. In both lines we observed a modest (30%) increase of Tbx5 in the postnatal atria. To gain insight into the effects of slight dosage increase in vivo, we investigated the atrial transcriptional, epigenetic and electrophysiological properties of both lines. Increased atrial Tbx5 expression was associated with induction of genes involved in development, ion transport and conduction, with increased susceptibility to atrial arrhythmias, and increased action potential duration of atrial cardiomyocytes. We identified an AF-associated variant in the human RE(int) that increases its transcriptional activity. Expression of the AF-associated transcription factor Prrx1 was induced in Tbx5RE(int)KO cardiomyocytes. We found that some of the transcriptional and functional changes in the atria caused by increased Tbx5 expression were normalized when reducing cardiac Prrx1 expression in Tbx5RE(int)KO mice, indicating an interaction between these two AF genes. We conclude that modest increases in expression of dose-dependent transcription factors, caused by common regulatory variants, significantly impact on the cardiac gene regulatory network and disease susceptibility.

New Jets Uniforms Coming Spring 2019 Eric Allen The Jets will have a brand new look in 2019. The franchise will unveil new uniforms next April and the Jets will wear their ensemble beginning in their 60th season of play. Nailed it. #JetsNewUnis https://t.co/lyJUf2uE7c — New York Jets (@nyjets) July 29, 2018 After starting as the Titans of New York and sporting a navy, gold and white combination from 1960-62, the team was renamed the Jets prior to the 1963 season and kelly green and white became synonymous with the upstarts from the American Football League. There have been three major uniform alterations since. The last one came prior to the 1998 campaign when Bill Parcells oversaw a color change from kelly green to hunter green and removed black as an accent color while paying homage to the Super Bowl III championship team with a modified version of the oval on the helmet that was used from 1965-77. Suspense will build in the months prior to a highly anticipated reveal. As far as all the details, we'll have to wait until April. But change is on the horizon. Note: There will be no changes to the current uniform for the upcoming season. The Jets will wear their "Color Rush" uniforms one time in 2018 as they'll be in all green when they host the Minnesota Vikings in Week 7. Jets TE Chris Herndon Began Year with 'Hiccups,' Finished with Flash Third-Year Player Showed Receiving Form Over Final 7 Games That He Displayed as a Rookie in 2018

Le Syndrome de Lemierre ou septicémie post-anginale ou encore nécrobacillose est une maladie rare mais grave causée principalement par la bactérie Fusobacterium necrophorum, qui est la deuxième cause d'angines bactériennes, après les streptocoques hémolytiques du groupe A. Son nom fait référence au bactériologiste français André Lemierre ; antérieurement, on l'appelait en raison de sa rareté, mais elle pourrait être plus commune qu'on le pensait Épidémiologie C'est une maladie devenue rare, puis dont l'incidence a recommencé à augmenter, ou qui n'est peut-être pas aussi rare qu'on le pensait, d'autant qu'il peut en exister des présentations atypiques. L'augmentation observée depuis quelques années pourrait être liée à l'antibiorésistance et/ou à des changements dans les schémas de prescription d'antibiotiques. Selon une revue d'étude (basée sur 84 études répondant aux critères d'inclusion) : Le rapport homme/femme était de 1: 1,2 ; les âges variaient de 2 mois à 78 ans selon le études (médiane, 22 ans) ; les lieux principaux d'infection étaient les amygdales, le pharynx et la poitrine ; Le tableau clinique le plus fréquent est un mal de gorge, suivi d'une masse au cou et d'une douleur au cou ; Fusobacterium necrophorum est le microbe le plus fréquemment associé ; les traitements anticoagulants et chirurgicaux étaient les plus utilisés ; La morbidité était et la majorité des patients subissent une longue hospitalisation. Taux de mortalité : 5% . Selon Riordan (2007), dans 10 % des cas, ce syndrome est associé à une mononucléose infectieuse, et il est possible qu'une première infection par un autre germe facilite la colonisation par le Fusobacterium necrophorum ou puisse l'aggraver. Le mode de contamination est inconnu. Diagnostic Le Syndrome de Lemierre associe fièvre, douleur cervicale et des symptômes pulmonaires. Il est aussi caractérisé par : une infection oropharyngée récente ; des preuves cliniques ou radiologiques de thrombose (thrombophlébite septique plus précisément) de la veine jugulaire interne entrainant des emboles septiques multiples ; l'isolement d'agents pathogènes anaérobies, principalement Fusobacterium necrophorum. Des complications ophtalmologiques sont parfois présentes Traitement La réussite de la prise en charge repose sur la conscience de la maladie, un indice de suspicion élevé et une approche multidisciplinaire du patient, généralement placé sous antibiotiques et sous anticoagulant. Voir aussi Article connexe Fusobactérie Fusobacterium necrophorum Noma (maladie), une stomatite nécrosante pouvant également être provoquée par Fusobacterium necrophorum Lien externe Syndrome de Lemierre sur Orpha.net Bibliographie Notes et références Maladie infectieuse Maladie rare Syndrome en pneumologie Syndrome en hématologie

Hi, my name is Duane Johnson, and i'm fighting government corruption on all levels. I'm working to make our country a better place. I'm exposing the unfairness in government and society. So watch my videos, and if you would like to support my efforts, I would appreciate it greatly. Patriot, a person who understands that the government employees are in fact SERVANTS!!! I am trying to reach as many people as I can, exposing the corruption in our government.

For people who have recently had a stent implanted in a blocked heart artery, the risk of developing a blood clot may be higher early in the morning than other times of day, researchers reported Monday. Stents are tiny, scaffold-like devices implanted into narrowed heart arteries to help improve blood flow to the heart. In about 1 to 2 percent of patients, a blood clot forms around the stent, which can trigger a heart attack or stroke; the risk is greatest in the few months after the stent is implanted. In the new study, researchers found that for people who developed a blood clot within a month of their stent procedure, symptoms were more likely to begin at 7 a.m. than at any other time of the day. Among the 49 patients, symptoms arose at 7 a.m. at least twice as often as any other hour of the day, according to findings published in the Journal of the American College of Cardiology: Cardiovascular Interventions. The study adds to evidence that the risk of heart problems varies according to the body's circadian rhythms, the 24-hour cycles that influence sleep, hormone production, body temperature and other essential functions. Past studies have found that the incidence of heart attack, cardiac arrest and stroke generally peak in the morning. "We expected to see circadian patterns and variations (in blood clot risk), because they are part of normal life," said Dr. David R. Holmes of the Mayo Clinic in Rochester, Minnesota, the senior researcher on the study. It's known that blood pressure, heart rate and the blood cells' "stickiness" tend to increase in the morning, all of which might contribute to the higher risk of stent blood clots, according to Holmes's team. A researcher not involved in the study called the findings "interesting" and said that future studies are needed to confirm the greater morning-time risk and uncover the reasons. "Identifying this circadian pattern helps focus attention on the possible reasons," said Dr. Geoffrey Tofler, a professor of preventative cardiology at the University of Sydney in Australia who has studied circadian rhythms and heart disease. But what can patients with newly implanted stents do for now? Holmes said they can be particularly attuned to potential symptoms of a blood clot early in the morning. Those include chest pain or discomfort and shortness of breath. There are also "atypical" symptoms, like nausea, Holmes noted, and elderly patients are more likely to have those compared with younger people. "If you wake up feeling 'strange,'" Holmes said, "maybe you're not just feeling strange, and it's something that needs medical attention." Still, since stent blood clots arise in about 1 to 2 percent of patients, the absolute risk to any one person would be low. And Holmes stressed that, in general, "stents are safe and effective, and very good at preventing symptoms." "But they are not fail-safe," he said. "And they need careful medical control." Stent patients generally take aspirin plus another anti-clotting medication for some time after the procedure, a year or more if they have drug-coated stents. So staying with your prescribed regimen is important, both Holmes and Tofler pointed out. The findings are based on 124 patients treated at the Mayo Clinic for a stent blood clot between 1995 and 2009. Of those patients, 49 had an "early" clot, within 30 days of having the stent implanted, while 30 developed a clot within a year, and 45 more than one year later. Only the risk of an early clot was elevated in the morning; later ones showed no clear circadian pattern. That, according to Holmes, may be because early and late blood clots happen for different reasons. The researchers were also able to pinpoint potential triggers of blood clots in some of the patients. About 6 percent had not been complying with their medication, and another 8 percent had infections or medical problems that could have either contributed to the clot or interfered with their medication regimen. In addition, 11 percent of the patients said they'd been performing heavy exercise before their symptoms arose something that is not advised. "It is generally recommended that people not perform heavy exertion in the initial weeks after receiving a stent, since this may increase risk of stent thrombosis (blood clot)," Tofler told Reuters Health by email. According to Holmes, the findings also raise the question of whether patients' anti-clotting medication doses may be wearing off by the early morning, and whether it might be better to split the dose into two, taken in the morning and night. Tofler, however, expressed doubts about that. The "duration of action" of both aspirin and clopidogrel (Plavix) the anti-clotting drug commonly given to stent patients is longer than one day, he said. "So unless people weren't taking those tablets when they should have been," Tofler said, "timing should not have been a problem."

Chinese spend billions in world's biggest online shopping day by Sophia Yan @sophia_yan November 11, 2014: 8:29 PM ET A look at Singles Day spending China's biggest annual shopping bonanza smashed records Tuesday as online sales on Alibaba alone hit $9.3 billion, more than the total racked up the same day last year across the industry. That followed a frenetic morning of shopping -- sales on Alibaba's e-shopping platforms exceeded $2 billion in just the first hour. The online shopping festival is called "Singles Day," and started as a way to celebrate single people. It's now morphed into a frenzy that attracts buyers both single and paired. Last year, Singles Day sales online totaled $8 billion on Alibaba and other platforms, more than double the combined $3 billion sold in the U.S. for Black Friday and Cyber Monday, according to the Boston Consulting Group. Alibaba (BABA) accounted for 75% of all online shopping on Singles Day last year. If that ratio is repeated this year, total sales will exceed $10 billion. People bought everything from dried cranberries to home appliances. Chinese smartphone maker Xiaomi said it sold 720,000 smartphones, bringing in $163 million in sales in 12 hours. Singles Day began as a cheeky antidote to Valentine's Day. It takes place each year on Nov. 11 -- or 11/11, representing four single people. On the day, it's common to exchange gifts and eat fried dough strips -- like a churro -- because they look like the number "1." Related: The ultimate guide to shopping on Thanksgiving Chinese tech giant Alibaba moved to capitalize on the shopping fervor back in 2009, when it began promoting special discounts to lure in more customers. Alibaba offers deals on everything -- food, apparel, electronics, home appliances, cosmetics and more. Last year, a 13-carat diamond was sold for nearly $4 million. This year, Alibaba went beyond China, partnering with sellers on its online marketplaces to lure shoppers around the globe with bargains. Customers from 217 countries and regions placed orders, with 43% of total online sales coming through mobile devices. How online purchases are delivered in China Although rival e-commerce firms such as JD.com also offer discounts and see a high volume of sales, much of the focus is on how Alibaba performs. This year's shopping holiday is the first since the firm posted its $25 billion record-breaking New York IPO. Alibaba shares dipped nearly 4% despite the bumper sales but are still up 24% since its debut. Related: Meet four kings of Alibaba's online retail empire Alibaba is on a firm footing -- revenue soared 54% in the three months ended September from a year earlier. Experts say the one-day bonanza is gradually having less impact on its annual e-commerce sales. Last year's Nov. 11 sales accounted for 4.8% of annual e-retailing revenue, versus 6.3% in 2012. That percentage "may be less this year, because online shopping has become more of a daily habit and less of a special occasion," said Jeff Walters of the Boston Consulting Group. China is already the second largest online retail market in the world, and the fastest growing. The industry brought in roughly $210 billion in 2012, and is expected to hit $650 billion by 2020, according to McKinsey. CNNMoney (Hong Kong) First published November 10, 2014: 8:38 PM ET

La strada europea E93 è una strada obsoleta della rete stradale internazionale. È stata riclassificata in strada europea E95. Altri progetti Collegamenti esterni Lista delle strade europee 093

A complex dynamic is at work in this relationship, with its equally strong forces of attraction and repulsion. The partners differ temperamentally, but share a need for extreme privacy and intimacy'' their relationship magnifies that need. Starching for beauty, they may look for it in each other or work together to find it in nature, an or humankind. They often overlook or forget their individual differences in this pursuit, accomplishing a fusion of extremely different personalities into a common whole. There is, of course, a danger here of a loss of individual try, hut these personalities are generally strong enough to survive within the relationship. Love affairs between these two will be difficult and trying. Emotional complexity and a curious inability to move the passionate relationship forward will underline the difficulties that these two have communicating with each other. Sagittarius-Capricorns will rend to turn silent and hurt, expecting their lover to understand them, while Gemini-Cancers may prefer to keep things cool, trying to avoid misunderstandings and problems altogether. Gemini-Cancer-Sagittarius-Capricorn work relationships, marriages and parent-child match ups have a great deal to offer co-workers, children and other family members in terms of varying and contrasting points. If view the children of such parents, for example, will have a genuine choice about which parent to approach with any problem, and will not expect a monolithic response. The relationship''s drive toward beauty and balance will usually exert a positive and harmonious influence on whatever group is a part of. Keep your eye on the object. Don''t get bogged down in feelings. Emphasize constructive activities. Appreciate your differences.

Q: PHP form creates a Postgresql entry when web page is opened. How can this be prevented? I have a php/html script to update my postgresql database. The form works alright, that is it inserts data into the database. Problem is that each time I open the web page to do an insert an empty entry is created into the database. I have tried to add a "SELECT setval(...)" to repair the sequence. This did have the effect of getting rid of empty rows it does however not prevent the sequence to get out of line. This is what I have got sofar: . . . <?php $db = pg_connect("host=localhost port=5432 dbname=crewdb user=postgres password=XXXXXX"); $query1 = "INSERT INTO access(access_name) VALUES ('$_POST[access_name]')"; $result1 = pg_query($query1); $query2 = "SELECT setval('access_accessid_seq', (SELECT MAX(accessid) FROM access))"; $result2 = pg_select($query2); pg_close($db) ?> However the problem still is not solved. I get a table like this: accessid | access_name ----------+-------------------------------------- 1 | good ride on mower access throughout 2 | n\a 3 | no ride on mower acces 4 | partial ride on mower acces 5 | fenced off 7 | Obtacles As you can see accessid is out of wack. Does anyone know how to solve this problem in the most efficient way? Table specs are: Column | Type | Collation | Nullable | Default | Storage | Stats target | Description -------------+---------+-----------+----------+------------------------------+----------+--------------+------------- accessid | integer | | not null | generated always as identity | plain | | access_name | text | | not null | | extended | | I am a bit new to this so thanks for your help.

Written especially for children aged 3 and over, these easy learning books from Collins are designed to give children a sense of achievement as they learn a range of skills. Supporting the National Curriculum, the activities will also help children boost their confidence, develop their learning skills and motivate them to want to find out more. A trusted brand when it comes to teaching children all about the key skills of school, these 15 books will prove essential as children begin to learn their A-Z.

Excellent stepped cubic crystals of Fluorite with phantoms from the Okorusu Flourspar Mine, Namibia. Crystals of Fluorite measure to 1.7cm on edge, with purple edges on an essentially colourless zone covering a translucent green cubic phantom with mottled areas of purple on the phantom. The crystals of Fluorite are pleasingly contrasted on the white Quartz crystal matrix. A neat specimen from the mine.

The RCMP Musical Ride represents a colourful Canadian tradition. The troop of 32 horses and riders will perform a variety of cavalry drills choreographed to music. Working through a unique medium, they support the RCMP community policing efforts throughout Canada. The Musical Ride was developed in 1876 by early members of the North-West Mounted Police to display their riding ability and entertain the local community. It has evolved over the decades into a uniquely Canadian performance enjoyed by tens of thousands of spectators every year. Members of the Musical Ride are first and foremost police officers. They all have at least two years experience in active police work before volunteering for duty with the Musical Ride. Surprisingly, most have no prior riding experience.

Evaluating the US Programme A new report looks at the broad impact of the Sutton Trust US Programme on participants, including on their ambitions and outlook. The report, by the Bridge Group, was commissioned to look at the impact of the programme beyond progression to highly selective US colleges. Through surveys and interviews, the report gives insights into programme participants' experience of studying in the US, the merits of a digitally delivered programme and the benefits of the programme on students who don't go on to study in the US. The Sutton Trust US Programme, run in conjunction with the US-UK Fulbright Commission, was launched in 2012 to support state school students to explore studying the US. Each year it gives a cohort of young people a taste of what it's like being an international student in the US to see if it could be the right fit for you. Participants who decide to apply to a US university receive support to navigate the application and financial aid process. Since 2012, the Sutton Trust has supported 1500 students through the US Programme. Of those 514 have gone on to study in the US, benefitting from over $125M in financial aid. Key findings from today's report include: Across all cohorts, participants reported a highly positive impact of the programme. They were most likely to say that the programme impacted their broader outlook and ambitions and their understanding of US culture, politics and society. Programme participants who decided not to apply to US colleges cited a preference for the UK higher education system as a key reason. Interviewees reported that participation in the programme had a broad positive impact on personal development, international outlook and skill development – regardless of whether they went on to a US or UK university. Interviewees reported life at US colleges as being tough but rewarding, with many interviewees appreciating the freedoms of a liberal arts education. However, participants reported having to navigate issues of racism and socio-economic inequality on campus; as well as mental health problems. Participants said that future participants would benefit from more support through the programme after starting their studies. This would help them to navigate some of the challenges they identified. The findings will inform future delivery of the programme and help to ensure that the programme offers the best possible experience for participants. The Sutton Trust and the Fulbright Commission have already begun offering more support for UK applicants through the Sutton Trust Online platform, developed and delivered bespoke programming on race and mental health, and are exploring further collaboration to support students once in the US through our respective alumni networks. This work will continue over the coming year to address the findings of the report and strengthen the programme as we continue to deliver to new cohorts of young people. Many thanks to the generosity of donors to the US Programme who have enabled this work and impact. To see the full report, please click here. Were 2021's sporting successes also wins for social mobility? Our Research and Policy Officer Erica Holt-White looks back at this year's sporting successes, and... Reflecting back on 2021 and looking forward to 2022 Our CEO, James Turner, sets out the Sutton Trust priorities for the new year. The Sutton Trust submits its representation to the Comprehensive Spending Review We call for more support for young people in the wake of the pandemic. If you're a journalist with a question about our work, get in touch with Hilary or Ruby. If it's out of office hours, you can call or text 07917 462 164. E: [email protected] T: 020 7802 1660 Subscribe to our monthly update

Dosage Charts Wilmington Pediatrics Book your child's flu shot online. Securely pay your bill online. Easily access and download forms online. Wilmington Pediatrics Providers Sarah Andrew, MD Dr. Andrew has been with Wilmington Pediatrics since 1990. She is board certified by the American Board of Pediatrics and is a fellow of the American Academy of Pediatrics. Dr. Andrew graduated from the University of Wisconsin and received her medical degree from Boston University School of Medicine. She completed her pediatric training at Massachusetts General Hospital. She enjoys all of pediatric medicine, but she especially loves guiding parents through their child's infancy and early childhood. Daniel M. Carson, MD Dr. Carson joined Wilmington Pediatrics in 1993. He is a fellow of the American Academy of Pediatrics and board certified by the American Board of Pediatrics. Dr. Carson graduated from Dartmouth College and received his medical degree from Boston University School of Medicine. He completed his pediatric training at the Floating Hospital for Children at Tufts Medical Center. He and his wife have four children. Dr. Carson enjoys all sports and outdoor activities. His other hobbies include golf, woodworking, reading, traveling and doing crossword puzzles. Gayathry S. Nene, DO Dr. Nene joined Wilmington Pediatrics in 2020 after completing her pediatric residency at Floating Hospital for Children at Tufts Medical Center. As an undergraduate at Tufts University she double majored in Child Development and Community Health. She then received her medical degree at the University of New England College of Osteopathic Medicine. Dr. Nene is a member of the American Academy of Pediatrics and the Massachusetts Medical Society. Her professional interests include all aspects of primary care, from working with new parents during the baby's first year of life to mentoring adolescents through their teenage years. Outside the office she loves dancing, traveling, baking, exploring new restaurants with her husband, and taking her toddler son swimming. Carolyn J. Sedor, MD- July 1, 2022 To my patients and families, I will be retiring from my position here at Wilmington Pediatrics as of November 1, 2022. Caring for young families, children and teens has been a privilege. I am inspired every day by the work parents do to raise healthy children, sometimes under very trying circumstances. Deciding to retire after 30 years here in Wilmington was difficult. I will, however, have more time to spend with my own children who now live in 3 different states. I am also looking forward to more outdoor activities including hiking and gardening. Your child's health is important to me. Please know the other providers Drs. Andrew, Carson, Nene, Stephens and Tanguay will still be here to care for you. The staff at Wilmington Pediatrics is easily available to answer questions and to help you transfer to one of our other Pediatricians. It has been a joy to be your pediatrician. Carolyn J. Sedor, MD Sandy Stephens, MD Dr. Sandy Stephens joined Wilmington Pediatrics in its infancy, in 1992. Originally from New York, she attended several art schools before landing at Skidmore College. There she discovered her passion for science and, through a work study program with developmentally delayed children, her love of children. After a brief foray in Massachusetts as a nanny for a chocolate consultant, she attended Albert Einstein College of Medicine. From the start, she was pulled towards pediatrics and continued to be inspired by the vast potential and resiliency of children and families. She decided to return to Boston and did her residency at Boston City Hospital (now Boston Medical Center), which prepared her for the multi-faceted challenges of pediatric health care. It was also where she met Drs. Sarah Andrew and Carolyn Sedor. Following the birth of her first child, she joined them at Wilmington Pediatrics and has enjoyed being a part of such a vibrant practice. As a general pediatrician, Dr Stephens adores babies just as much as she loves guiding teenagers through adolescence. She is board certified by the American Board of Pediatrics, a fellow of the American Academy of Pediatrics and a member of the Massachusetts Medical Society. Currently, she serves as the Medical Director of Wilmington Pediatrics. Dr. Stephens is a dedicated mom, wife and pet owner who is rarely without her Kindle, yet finds a myriad of way to nurture her artsy side. Normand A. Tanguay, PhD, MD Normand Tanguay, PhD, MD (he, him, his) joined Wilmington Pediatrics in 1996. He went to medical school at McGill University after earning a PhD in Physical Chemistry at Brown University in 1993. He had always been interested in science and technology but while at Brown he decided that he wanted a career with more direct people interaction as well. This led him to medical school and a residency in pediatrics at The Floating Hospital for Children at Tufts Medical Center. Dr. Tanguay (he, him, his) is involved in the larger medical community. He is the Chair of the Department of Pediatrics at Winchester Hospital and is the medical director of the Health Information Exchange. He is married to Peter, who is a cardiologist. They have two teenagers. While not a natural athlete, he and Peter run regularly and he is very proud to have completed the Spartan Fenway Park race in 2015. He also enjoys the challenge of cooking for his family which consists of 2 vegetarians, a vegan and himself. Dr. Nicole Rittershaus joined Wilmington Pediatrics in 2022. Prior to joining, she was a general pediatrician in Boston and the Merrimack Valley. She is a fellow of the American Academy of Pediatrics and board certified by the American Board of Pediatrics. Dr. Rittershaus graduated from Brown University where she majored in Human Biology, brain and behavior and received her medical degree from Brown University School of Medicine. She completed her pediatric training at the Floating Hospital for Children at Tufts Medical Center. She loves primary care and has a special interest in adolescent health, mental health, and medical education. In her free time, she enjoys spending time with her husband, young son and 2 spunky rescue dogs. She also enjoys long distance jogging, playing the viola, and reading fiction. View Dosage Charts View helpful information when dosing Acetaminophen, Ibuprofen, & Benadryl View immunization guidelines recommended by the American Academy of Pediatrics Copyright 2023 Wilmington Pediatrics. Medical Website Design by practis, inc. : Privacy Policy : Notice of Non Discrimination

S Korea turns to tourism & tech as it seeks economic normalcy in 2022 The government of South Korea has reportedly announced its ambitions to achieve full economic normalcy beyond the coronavirus pandemic in 2022, and also take lead in the global competition to predominantly occupy future businesses. On Monday, South Korea's Finance Minister and Deputy Prime Minister, Hong Nam-ki, emphasized these primary objectives during the government's announcement of economic policy objectives for the coming year at the Government Complex in Seoul. In order to achieve maximum economic normality, the Ministry of Economy and Finance has come to a decision to implement policies to increase private spending, with a vision to promote the tourism industry, which has been dramatically affected by COVID-19. According to the ministry, the government will offer a plethora of benefits to outbound tourists, which will undoubtedly be requested in conjunction with the future condition of the Covid-19 outbreak involving quarantine. The intention is to eliminate the $5,000 (5.9 million won) upper limit on purchases at duty-free establishments across the country as a notable benefit. Previously, the price ceiling for outbound tourist (Koreans) was raised from $3,000, which had been authorized since 2006, to $5,000 in 2019. Additionally, the non-landing flight tour products' operational time will be extended from 'till the end of 2021' through 'June 2022.' In fact, the number of overseas flight routes will be aggressively increased or recovered, from the existing 70 routes to over 31 countries, approximately 330 weekly flights. Prior to the pandemic, 4,712 flights served 255 routes across 45 nations. In collaboration with local governments, the development of national tour products will be boosted. As per finance minister Nam-ki, the tour promotions would be extended, on the basis of consistently stabilizing consumer spending and aiding individuals affected by the pandemic, including micro-business owners. Meanwhile, in February, the government would also classify 65 technologies in three categories, namely, semiconductors, batteries, and vaccines as 'national strategic technologies', the first of its kind in relation to government policy. According to the Finance Ministry, the GDP will grow by 4% in 2021 and 3.1 percent in 2022. This is greater than growth forecasts from some independent research organizations, which predicted growth of under 4% this year as well as 2% next year. Source credit: http://www.koreaherald.com/view.php?ud=20211220000701&np=1&mp=1 Date: Dec 21, 2021 Rini Dalvi

Oiltanking Antwerp Gas Terminal inaugurates the largest butane storage tank in Europe go to press photo archive Oiltanking Antwerp Gas Terminal N.V. (OTAGT) successfully commissioned a fully refrigerated, double containment tank of 135,000 cbm, thereby doubling its storage capacity. The tank is designed to store butane on behalf of INEOS and marks a first step in the terminal's expansion plans. A second fully refrigerated tank with similar storage dimensions is currently under construction at OTAGT. With a diameter of 87 meters and a height of 59 meters, the tank does not go unnoticed when visiting the left bank of the port of Antwerp. The order of magnitude of the project becomes apparent when taking note of some facts and figures. The tank structure contains approximately 43,600 cbm of concrete - delivered by 5,400 trucks - and 9,750 tons of steel. After 950,000 contractor working hours, 3,600 toolbox meetings and over 2,600 safety walks, the tank was ready to put into service. Douglas van der Wiel, Senior Vice President EMEA, comments: "By completing this project, Oiltanking reiterates its capability as a world class service provider for gas logistics. The Oiltanking brand represents quality backed by highly experienced and skilled people, working together as a team. This project had its challenges along the way, but the project team managed to progress the construction in accordance with all applicable standards and more importantly in a safe way. We would obviously like to thank INEOS for its continued trust in our company as well as our employees, our main contractor TGE, and all other stakeholders involved for the hard work put in enabling us to reach this milestone." OTAGT herewith reaffirms its position as leading gas storage hub within the ARA region (Antwerp-Rotterdam-Amsterdam). Oiltanking GmbH acquired OTAGT in 2016 in light of its strategy to further expand its chemicals and gas assets portfolio. Apart from being located in the petrochemical heart of Europe, the terminal is heavily integrated with the surrounding industry and connected to all major pipeline networks. Oiltanking Antwerp Gas Terminal NV, a fully-owned subsidiary of Oiltanking GmbH, is located in the port of Antwerp and is one of the largest independent terminals in Europe for storage, throughput and distribution of LPGís and petrochemical gases. Oiltanking GmbH is a subsidiary of Marquard & Bahls, a Hamburg-based company that operates in the fields of supply, trading and logistics of energy & chemicals. Oiltanking is one of the largest independent tank storage providers for petroleum products, gases and chemicals worldwide. The company owns and operates 64 terminals in 24 countries in the Americas, Europe, Middle East, Africa, and Asia-Pacific including China and India. Oiltanking has an overall storage capacity of 20.2 million cbm. +49 40 37004-0+49 40 37004-7609 Nancy De Groof Vice President Business Development EMEA +32 472 810857E-Mail

Q: The repository primary signature validity period has expired I am getting this error in the Build and Deploy job phase of an Azure Static Web Apps CI/CD check-in. The first part of the error message is: Run cd UnoPhotoViewer/UnoPhotoViewer.Wasm Microsoft (R) Build Engine version 16.8.3+39993bd9d for .NET Copyright (C) Microsoft Corporation. All rights reserved. Determining projects to restore... /home/runner/work/PhotoViewer/PhotoViewer/UnoPhotoViewer/UnoPhotoViewer.Wasm/UnoPhotoViewer.Wasm.csproj : error NU3028: Package 'runtime.rhel.7-x64.runtime.native.System.Security.Cryptography.OpenSsl 4.3.0' from source 'https://api.nuget.org/v3/index.json': The repository primary signature's timestamp found a chain building issue: UntrustedRoot: self signed certificate in certificate chain /home/runner/work/PhotoViewer/PhotoViewer/UnoPhotoViewer/UnoPhotoViewer.Wasm/UnoPhotoViewer.Wasm.csproj : error NU3037: Package 'runtime.rhel.7-x64.runtime.native.System.Security.Cryptography.OpenSsl 4.3.0' from source 'https://api.nuget.org/v3/index.json': The repository primary signature validity period has expired. It appears that all project nuget dependencies are throwing similar messages. My Nuget Package Manager has both Allow Nuget to download missing packages AND Automatically check for missing packages during build in Visual Studio CHECKED. Does anyone know what is causing this and how to resolve? Thanks

The American Bell Association International, Inc. (ABA) is a nonprofit organization devoted to the collection, preservation, restoration, and research of bells in which members can attend regional chapter events and an annual national convention. Twenty-two U.S. chapters and an additional five international chapters are recognized by the American Bell Association International; global membership is 1,200 persons. The organization is one of over 100 names that uses the acronym ABA. History The American Bell Association International started in 1940 as the National Bell Collector's Club by several women staying at the Chautauqua Institution in New York State, and the organization initiated a monthly printed publication, The Bell Tower, named in honor of the Miller Bell Tower at the institution. In 1989 The Bell Tower became a bi-monthly printed publication and has remained so since. Content of The Bell Tower is varied, ranging from miniature collectible bells to huge tower bells. Events The first American Bell Association International annual convention took place in Chicago, Illinois, in 1946. The fifth annual convention was held in New York City and featured in The New Yorker, which covered one of the American Bell Association International's founders, Mary A. Collins, and Bells of Sarna by the founder of present day company S.S. Sarna. The most recent (2015) annual convention will be held in Kansas City, MO. The annual convention is held in a different state each year. Chapters of the American Bell Association International have various meeting schedules and convene two or more times per year. Chapter meetings generally include five main components. A social phase allows members to meet and converse. Presentations allow members and/or experts to disseminate information about a focus or discipline regarding bells and may take such forms as digital and/or oral lectures. A business discussion phase addresses chapter goals and achievements. A display period allows members to provide viewings of items from their bell collections, and an exchange period presents members the opportunity to buy, sell, and/or trade items. See also Bell of King Seongdeok Bellfounding Bianzhong Campanology Carillon Change ringing Glockenmuseum Stiftskirche Herrenberg Jingle bell Liberty Bell List of heaviest bells Russian Orthodox bell ringing Ship's bell Tsar Bell Notes American Bell Association International members include(d) authors of books on travel to find bells, collecting bells as a hobby, bells as culturally significant instruments, and campanology. The American Bell Association International is recommended by the Smithsonian Institution as the source from which public inquiries may obtain information about bells. The symbol of The American Bell Association International is a bell reported by the donor (the organization's 14th president) to have belonged to Ulysses S. Grant. References External links The American Bell Association International, Inc. 'Bell Talk' Bells (percussion) Clubs and societies in the United States

The employees are at the heart of a company. For many organizations, personnel costs account for a large part of their total operating expenses. Things can become very difficult if the wrong workforce planning decisions are taken, whether because of erroneous calculations or because the overview has been lost. The fact remains: successful workforce planning is a complex matter that is dependent on many factors. In this blog we explain how companies can efficiently and accurately calculate their personnel requirements. Workforce planning primarily comprises a forecast of the future workforce required to keep the business operational and profitable. This requirement depends on various latent factors: What is the short to long-term workload? What are the short-term growth prospects for the company? What staff shortages (due to illness, vacation, etc.) or seasonal fluctuations can be expected? At what locations should the different teams of employees be based? What are the different employee cost drivers dependent on the different locations and tax jurisdictions? What will be the impact of inflation or general salary increase to the overall salary expenses? The interplay between these factors and the resulting calculations are complex and have a direct impact on the relevant financial figures. Although most financial and workforce planners are aware of this complexity, they still do not attach enough importance to the coordinated and integrated planning of personnel costs. Many companies also still use Excel spreadsheets that have not been reconciled for this task, which makes it difficult to integrate the different business areas and to obtain a consistent, accurate and transparent overview. Personnel costs are not integrated into the company's financial core model (income statement, balance sheet, cash flow). In summary, then: most companies lack an integrated system of workforce planning that can react quickly to changing parameters and is aligned to the company's overall financial planning. Integrated workforce planning is dependent on a central database containing all the relevant and up-to-date data on the employees. This task can be handled by software solutions offering pre-defined applications with a simple feed-in process that collate all the required data in a single trusted hub. The advantage of such tools: they are standardized and make the relevant data, costs and drivers completely transparent to everybody who has access to the tool. Once the personnel costs have been defined as a key driver for the company, these costs are automatically integrated into all other financial calculations. This allows the unrestricted calculation of workforce requirements across different departments and divisions. The resulting uniform calculations not only improve the accuracy and comparability of the results, but also enable exact simulations and scenario calculations. And this is the beginning and end of successful human resources management. Would you like to see how the Apliqo Workforce Planning Solution helps you to overcome the common workforce planning challenges? Click HERE to watch the recorded version of our Webinar on integrated workforce planning.

Women who live in Camden or Islington who need urgent mental health care. Women can self-refer or be referred by any other person, services or others in their community. Referrals are only taken over the phone and can be made 24hrs a day.

Federal Register, Volume 84 Issue 72 (Monday, April 15, 2019) [Federal Register Volume 84, Number 72 (Monday, April 15, 2019)] Agency Information Collection Activities Under OMB Review SUMMARY: In compliance with the Paperwork Reduction Act of 1995 (``PRA''), this notice announces that the Information Collection Request (``ICR'') abstracted below has been forwarded to the Office of Management and Budget (``OMB'') for review and comment. The ICR costs and burdens. DATES: Comments must be submitted on or before May 15, 2019. ADDRESSES: Comments regarding the burden estimate or any other aspect of the information collection, including suggestions for reducing the burden, may be submitted directly to the Office of Information and Regulatory Affairs (``OIRA'') in OMB within 30 days of the publication of this notice, by either of the following methods. Please identify the comments by ``OMB Control No. 3038-0094.'' By email addressed to: OIRA [email protected] or By mail addressed to: The Office of Information and Regulatory Affairs, Office of Management and Budget, Attention: Desk Officer for the Commodity Futures Trading Commission, 725 17th Street NW, Washington, DC 20503. A copy of all comments submitted to OIRA should be sent to the Commodity Futures Trading Commission (``CFTC'' or ``Commission'') by any of the following methods. The copies sent to the Commission also should refer to ``OMB Control No. 3038-0094.'' Lafayette Centre, 1155 21st Street NW, Washington, DC 20581; By Hand Delivery/Courier to the same address; or Through the Commission's website at http://comments.cftc.gov. Please follow the instructions for submitting comments through the website. A copy of the supporting statement for the collection of information discussed herein may be obtained by visiting http://RegInfo.gov. http://www.cftc.gov. You should submit only information that you wish Freedom of Information Act, a petition for confidential information of the exempt information may be submitted according to the procedures established in Sec. 145.9 of the Commission's regulations.\1\ The Commission reserves the right, but shall have no obligation to review, pre-screen, filter, redact, refuse or remove any or all of your submission from http://www.cftc.gov that it may deem to be inappropriate for publication, such as obscene language. All will be considered as required under the Administrative Procedure Act and other applicable laws, and may be accessible under the Freedom of Information Act. FOR FURTHER INFORMATION CONTACT: Jocelyn Partridge, Special Counsel, Division of Clearing and Risk, Commodity Futures Trading Commission, Title: Clearing Member Risk Management (OMB Control No. 3038-0094). This is a request for extension of a currently approved information Abstract: Section 3(b) of the Commodity Exchange Act (``Act'' or ``CEA'') provides that one of the purposes of the Act is to ensure the financial integrity of all transactions subject to the Act and to avoid systemic risk. Section 8a(5) authorizes the Commission to promulgate such regulations that it believes are reasonably necessary to effectuate any of the provisions or to accomplish any of the purposes of the Act. Risk management systems are critical to the avoidance of systemic risks. Section 4s(j)(2) requires each Swap Dealer (``SD'') and Major Swap Participant (``MSP'') to have risk management systems adequate for managing its business. Section 4s(j)(4) requires each SD and MSP to have internal systems and procedures to perform any of the functions set forth in Section 4s. Section 4d requires FCMs to register with the Commodity Futures Trading Commission (``Commission''). It further requires Futures Commission Merchants (``FCMs'') to segregate customer funds. Section 4f requires FCMs to maintain certain levels of capital. Section 4g establishes reporting and recordkeeping requirements for FCMs. Pursuant to these provisions, the Commission adopted Sec. 1.73 which applies to clearing members that are FCMs and Sec. 23.609 which applies to clearing members that are SDs or MSPs. These provisions require these clearing members to have procedures to limit the financial risks they incur as a result of clearing trades and liquid resources to meet the obligations that arise. The regulations require clearing members to: (1) Establish credit and market risk-based limits based on position size, order size, margin requirements, or similar factors; (2) use automated means to screen orders for compliance with the risk-based limits; (3) monitor for adherence to the risk-based limits intra-day and overnight; (4) conduct stress tests of all positions in the proprietary account and all positions in any customer account that could pose material risk to the futures commission merchant at least once per week; (5) evaluate its ability to meet initial margin requirements at least once per week; (6) evaluate its ability to meet variation margin requirements in cash at least once per week; (7) evaluate its ability to liquidate the positions it clears in an orderly manner, and estimate the cost of the liquidation at least once per month; and (8) test all lines of credit at least once per quarter. Each of these items has been observed by Commission staff as an element of an existing sound risk management program at an SD, MSP, or FCM. The Commission regulations require each clearing member to establish written procedures to comply with this regulation and to keep records documenting its compliance. The information collection obligations imposed by the regulations are necessary to implement certain provisions of the CEA, including ensuring that registrants exercise effective risk management and for the efficient operation of trading venues among SDs, MSPs, and FCMs. currently valid OMB control number.\2\ On November 14, 2018, the Notice''). The Commission did not receive any relevant comments. Accordingly, it did not alter the burden estimates set forth in the 60- Day Notice. Burden Statement: The respondent burden for this collection is estimated to average 2 hours per response for an estimated annual burden of 504 hours per respondent. This estimate includes the total time, effort, or financial resources expended by persons to generate, maintain, retain, disclose, or provide information to or for a federal Respondents/Affected Entities: Clearing member Swap Dealers, Major Swap Participants, and Futures Commission Merchants. Estimated Number of Respondents: 166 (101 Clearing Member Swap Dealers and 65 Clearing Member Futures Commission Merchants). Estimated Average Burden Hours per Respondent: 504. Estimated Total Annual Burden Hours: 83,664 hours. Frequency of Collection: As needed. There are no capital costs or operating and maintenance costs associated with this collection. (Authority: 44 U.S.C. 3501 et seq.) Dated: April 9, 2019.

ADF Offers Free Legal Representation to Those Who Refuse to Recognize Iowa's Gay Marriages By Kyle Mantyla | April 23, 2009 12:41 pm The Alliance Defense Fund has long been one of the leading right-wing forces behind the so-called "right of conscience" designed to protect medical professionals for having to engage in practices that they feel would violate their "deeply-held religious beliefs." It has usually taken the form of ADF defending pharmacists who refuse to sell birth control on the grounds that it is a type of abortion, but it now seems to be expanding its definition to include county employees in Iowa who might refuse to abide by the recent state Supreme Court ruling mandating marriage equality by announcing that it will offer free legal defense to any county recorder who refuses to grant marriage licenses to same sex-couples in order to protect the "right of conscience … against heavy-handed coercion by the state [and serve] as the first line of defense against the cancer of tyranny": A letter issued by the Alliance Defense Fund and the Iowa Family Policy Center Wednesday offers free legal defense by ADF attorneys to county recorder offices that adopt a policy protecting employee rights of conscience. The policy would protect objecting employees from being forced to issue marriage licenses to same-sex couples if doing so would violate the employee's conscience. "Government employees who believe in marriage as the union of one man and one woman should not be penalized for abiding by their beliefs," said ADF Senior Legal Counsel Doug Napier. "This policy allows an employee who does not wish to violate his or her own conscience by issuing a marriage license to a same-sex couple to abstain and allow the transaction to be performed by someone who is willing to do it. Forcing them to participate in offensive acts contrary to their deeply held beliefs in order to remain employed is unconstitutional." Tags: Abortion Iowa Legal marriage Marriage Equality Supreme Court union Alliance Defense Fund Idaho GOPer: No Marriage Licenses For Anyone Because Gay Marriage Is Legal Todd Starnes Warns Pastors Who Refuse To Perform Gay Marriages To 'Prepare For Hate Crime Charges' Ben Carson: Federal Government Doesn't Need To Recognize Gay Marriage SCOTUS Ruling Sandy Rios: 'You Must Prepare For Martyrdom' If Gay Marriage Becomes Legal

Beverly Hills teuscher is here to stay! Serving the teuscher family's hand-crafted Swiss truffles and chocolate is a great honor… we look forward to making new memories and new friends who cherish the small batch, fine Swiss chocolates as much as we do! World renowned for the care and craftsmanship that are the hallmarks of teuscher.

Now that I'm back from my trip to Australia, the regular speedlinks on Wednesday can resume as per normal. Last week, I highlighted some of my Outback accommodations, so I thought it would only be fitting to dedicate this week's roundup to the concept of travel… even if it might mean never leaving your living room. We start off with a vacation post from Betshopboy, who takes us on a journey to Bali Safari and Marine Park. This destination combines, as you can imagine, a wildlife park with a water park. You can get up close and personal with such animals as komodo dragons and cheetahs, then relax with some waterslides and wading pools. Looks like a great time in the sun! Next, Jane Meighan has a guest post up on Problogger, talking about how to keep track of your accounts as a remote blogger. The great thing about making money online is that you can do it anywhere with an Internet connection, but you still have to have a system in place for managing your finances and other pertinent information. What about more permanent travel? RT Cunningham has been living in the Philippines for nearly six years and now he is considering a move back to the United States. There are many considerations at play, like affordability and convenience. He also has to consider what he'd do with his house and car, as well as how he'd handle relative proximity to family. Tough call. It wouldn't be fair to say that Raul Pacheco-Vega went traveling when he put his blog on temporary hiatus, but he did "move away" from the site in some ways. Now that a month has passed, he reflects on his experience with the hiatus and how he spent his time. Blogging is a way of life, so it's good to see that he's giving change a try. And finally, John Bardinelli decided to travel back in time by trying Chrono Trigger on iOS. How did the classic game transition onto the newer piece of gadgetry? According to John, not so well. The visuals are blurry, the controls are awful, and the high price tag is a definite turnoff. Some retro goodness should perhaps stay retro.

Smarter Living Home / Smarter Living / 65 Fun Christmas Facts to Get You in the Holiday Spirit 65 Fun Christmas Facts to Get You in the Holiday Spirit 'Tis the season for Christmas trivia! Here are some festive facts your whole family can enjoy. By Desirée O The holidays are drawing closer, and that means it's time to finish up your shopping, trim your tree, and get those Christmas greetings in the mail. It's been another long year, so you may need an extra dose of festive spirit this season. If that's the case, or if you're in need of some great icebreakers for family gatherings, read on for 65 fun Christmas facts that'll fill you—and anyone you're chatting with—with the magic of the holidays! RELATED: 30 Amazing Christmas Trees Facts to Make the Holidays Extra Magical. Sweden celebrates Christmas with a giant, straw Yule Goat. MATS ASTRAND/TT/AFP via Getty Images In Scandinavian countries, the Yule goat figures into the Christmas decor of many homes. In Norse mythology, the two goats pulled Thor's flying chariot the same way reindeer do for Santa's sleigh. On top of that, there's also a festive tale that focuses on an invisible Yule goat that checks in on holiday prep before the big day. That's why, in 1966, the Gävle Goat was created in an effort to draw shoppers to the Swedish city where it resides. Standing 42.6 feet high and seven meters in length, it also weighs a whopping 3.6 tons. Ralphie from A Christmas Story was also in Elf. Back in 1983, a young Peter Billingsley starred in A Christmas Story as Ralphie, a typical suburban kid who celebrates a holiday filled with hilarious ups and downs with his family. In 2003, Billingsley then popped up in another seasonal classic, Elf, as Ming Ming the toy-making supervisor in Santa's workshop. He's also appeared in other festive and non-festive films, including Four Christmases, Iron Man, and Spider-Man: Far from Home. RELATED: This Is What Ralphie From A Christmas Story Looks Like Now. Two of Santa's reindeer originally had different names. Vladimir Melnikov/Shutterstock In the 1823 poem, A Visit from Saint Nicholas, which originally introduced the world to Santa's reindeer, two of the flying creatures had slightly different names, according to Syracuse.com. Donner and Blitzen were instead Dunder and Blixem, which is a Dutch way to refer to "thunder and lightning." The longest-running Santa Claus parade happens in Illinois. Sergei Bachlakov/Shutterstock Plenty of communities hold Santa Claus parades, and each one is special in its own way. However, the parade that happens each year in Peoria, Illinois, is the oldest parade of its kind in the U.S. It's taken place every holiday season since 1888—that's well over 100 years of festive fun! One of the oldest Christmas markets dates back to the 1600s. CHRISTOF STACHE/AFP via Getty Images If you do a little shopping at the Christmas Market in Nuremberg, Germany, you'll be taking part in a tradition that's hundreds of years in the making. The proof of that can be found in a small box that the Germanisches Nationalmuseum keeps safe. The simple treasure holds an inscription that reads: "Sent to Regina Susanna Harßdörfferin by Miss Susanna Eleonora Erbsin (or Elbsin) on the occasion of the Christmas Market of 1628." "Rockin' Around the Christmas Tree" was recorded by a 13-year-old. GAB Archive/Redferns "Rockin' Around the Christmas Tree" is a classic holiday song that was released back in 1958 with Brenda Lee singing the festive tune. Billboard notes that, when the song was recorded, Lee was only 13 years old. In the years since, it's been re-recorded by stars including Kacey Musgraves, Justin Bieber, and Miley Cyrus. Artificial Christmas trees were once made with goose feathers. Krasula/Shutterstock These days, artificial Christmas trees are made of materials that are meant to mimic real evergreens and last for as long as possible. But when these fake festive trees were first displayed in Germany around 1865, they were made of goose feathers that were dyed green. Hallmark holiday movies only take 2 weeks to film. Some Hollywood movies take a month to shoot, others quite a bit more. On the other end of the spectrum, the super-popular holiday movies that Hallmark is able to put out at such an impressive pace only take two weeks to film, according to E! News. Brazil set the record for the world's largest floating Christmas tree. ANTONIO SCORZA/AFP via Getty Images At a staggering 278 feet and 10 inches tall and glowing with almost three million lights, Brazil's massive floating metal Christmas tree set a world record in December 2007. The tree continues to be brought out each year as a part of Rio De Janeiro's Rodrigo de Freitas Lagoon. Christmas could have been a spring holiday. A long time ago, Pope Julius I decided that Christmas should be celebrated on Dec. 25, however, History points out it wasn't because that was the date that Jesus was born. Instead, many people believe it was to overshadow a pagan festival called Saturnalia which also happened on that day. In fact, Jesus may have actually been born during the spring! "Jingle Bells" was the first song played in space. It's hard to imagine what you'd want to rock out to if you were to ever float among the stars. But the crew of NASA's Gemini 6A space flight got into the Christmas spirit and made history when they played "Jingle Bells" on December 16, 1965, earning the jolly jingle the Guinness World Record for being the first song ever played in space. Well, as far we know, at least… And it was originally a Thanksgiving song! "Jingle Bells" may be one of the most well-known Christmas songs, but it was originally composed as a Thanksgiving tune. Written by Unitarian church organist James Lord Pierpont in the 1850s, in Savannah, Georgia, the song was "first performed during a Thanksgiving concert at [his] church," Time explains. The tradition of hanging stockings comes from a legend about marriage. Have you ever wondered why we hang up stockings—which are basically fancy socks—during the holiday season only to wake up on Christmas morning to find them filled with tiny gifts? According to the Smithsonian, one of the most popular legends about the tradition's origins is the tale of a poor widower who worried that he wouldn't be able to marry off his three daughters because of his lack of wealth.ae0fcc31ae342fd3a1346ebb1f342fcb Fortunately, Smithsonian explains, "St. Nicholas was wandering through the town where the man lived and heard villagers discussing that family's plight. He wanted to help but knew the man would refuse any kind of charity directly. Instead, one night, he slid down the chimney of the family's house and filled the girls' recently laundered stockings, which happened to be drying by the fire, with gold coins. And then he disappeared." In the morning, the family found the gifts, and the daughters became eligible to wed. 'Twas a Christmas miracle! The NORAD Santa Tracker was created due to a child's misunderstanding. The North American Aerospace Defense Command, or NORAD, has been tracking Santa's journey around the world since 1958. According to their website, the innovation was set into motion in 1955, when "a young child [accidentally] dialed the unlisted phone number of the Continental Air Defense Command (CONAD) Operations Center in Colorado Springs, Colorado, believing she was calling Santa Claus after seeing a promotion in a local newspaper." The commander on duty, Colonel Harry Shoup, saw an opportunity to create a little Christmas magic, and assured the youngster that CONAD would guarantee Santa a safe journey from the North Pole, sparking the idea to track Santa's travels each year to the delight of children all over the world. If you gave all the gifts listed in the "Twelve Days of Christmas," it would equal 364 presents. Sing along: "On the first day of Christmas my true love sent to me / A partridge in a pear tree." Let's keep going! "On the second day of Christmas my true love sent to me / Two turtle doves, and / A partridge in a pear tree." If you continue to sing the famous festive tune and count up all of the gifts that are bestowed upon the lucky singer in the lyrics—French hens, turtle doves, calling birds, and so much more—you'd end up with 364 presents. For more seasonal trivia sent right to your inbox, sign up for our daily newsletter. In Ukraine, spiders are considered symbols of good luck at Christmas. When you think about Christmas creatures, reindeer and polar bears probably jump to mind… but what about spiders? In Ukraine, families often add spider web ornaments to their trees as a nod to a lovely seasonal story about the critters once using their silky string to decorate the tree of a poor widow and her children. Santa has a postal code in Canada. If you are in Canada and want to become pen pals with Santa Claus, you can send him a letter via the Canada Post mail system. The address? Santa Claus, North Pole, HOH OHO, Canada. And don't be surprised when you get a letter back! He has some helpers who keep up with his correspondence. There is a Christmas tree in Spain worth $15 million. Most of us have Christmas decorations that we hold dear for sentimental reasons, but the ornaments that adorn the Christmas tree at the Kempinski Hotel Bahia, near Marbella, Spain, have a different type of value. According to CNN, the tree is festooned with red, white, pink, and black diamonds, as well as jewelry from Bulgari, Cartier, Van Cleef & Arpels, and Chanel. And just for good measure, you'll also find 3D-printed chocolate peacocks, feathers, ostrich eggs, and bottles of perfume on the tree, which is worth a whopping $15 million! The Christmas tree pickle is a tradition to keep eager kids calm on Christmas morning. According to Today, the Christmas pickle is more than just a common ornament, it's a tradition. As the quirky tradition goes, the first child to find the glass pickle hidden in the tree on Christmas morning either wins a prize or the privilege of opening the first gift. The goal? To keep kids from rushing through the process of opening presents, and instead take the time to enjoy each one. Rudolph the Red-Nosed Reindeer was originally created as an advertising gimmick. Rudolph the Red-Nosed Reindeer has been a popular holiday character for some time now, and one that many recognize from the beloved 1964 TV movie of the same name. However, according to the Smithsonian Insider, "Rudolph the Red-Nosed Reindeer first appeared in 1939 when Montgomery Ward department store asked one of its copywriters, 34-year-old Robert L. May, to create a Christmas story the store could give away to shoppers as a promotional gimmick." And thus Rudolph was born, with Montgomery Ward distributing 2.4 million copies of the story in the first year of its publication. Eggnog dates back to medieval times. Eggnog is a signature beverage of the holiday season, and one that has a history dating all the way back to medieval times and a drink called "posset." Made with sweetened and spiced milk curdled with ale or wine and served hot, according to Healthline, monks in the 13th century "enjoyed this mixture with the addition of eggs and figs." Tinsel was once made of real silver. Throwing some tinsel on your Christmas tree is a quick and inexpensive way to jazz up your holiday décor, and has been for years. However, it used to be a much pricier adornment. According to the BBC, tinsel has its origins in the early 1600s in Nuremberg, Germany, where people "used thin strands of real silver in their trees to reflect candlelight, as they used to put real candles in their trees." And since silver was expensive, being able to use tinsel in your tree was something of a status symbol. Americans expect to spend around $837 on Christmas gifts this year. In 2021, U.S. consumers on average are expected to spend $837 on gifts this holiday season, according to a Gallup poll. This shows that expected spending bounced back a bit from 2020 but still won't be on par with the pre-pandemic average of $850. But they actually end up spending nearly $1,500 on presents. Despite intent to stick to a Christmas-gift budget that's under $1,000, most Americans tend to go a little overboard. In an October 2019 study, Deloitte found that U.S. consumers spend around $1,496 during the giving season. The Statue of Liberty is the largest Christmas gift ever given. Standing over 150 feet tall and weighing 225 tons, the Statue of Liberty is an impressive figure. And since it was considered a holiday gift when it was given to the U.S. by France in 1886, it's officially the largest Christmas present in the world. That's a lot of wrapping paper and ribbon! Thomas Edison and his business partner invented Christmas lights. Thomas Edison has gone down in history as one of America's greatest inventors. We can thank him for the light bulb, phonograph, and even the movie camera. But did you know he is also partly responsible, along with his friend Edward H. Johnson, for inventing Christmas lights? According to the Library of Congress, Edison created the first strand of electric lights in 1880, which he hung outside his laboratory in Menlo Park, New Jersey, during Christmastime that year. However, it was Johnson, his partner at Edison Illumination Company, who, in 1882, became the first to wrap a strand of hand-wired red, white, and blue bulbs around a Christmas tree. And the rest, as they say, is history! The first tree at Rockefeller Center was an unadorned tree from construction workers. The first Christmas tree at Rockefeller Center in New York City went up in 1931. However, it was hardly the big spectacle it is today. Instead, it was a 20-foot tree with no ornaments that was set up by construction workers who were laboring at the center's construction site. A second tree went up two years later, this time a 50-footer with lights, sparking the annual tradition. Today, the Rockefeller Center Christmas tree features more than 25,000 lights. Today, if you visit Rockefeller Center during the Christmas season, you'll surely be blown away by the sight of the sparkling tree. The towering tree is decorated each year with over 25,000 lights, according to Time. Can you imagine checking every bulb to see if it works?! The first Christmas card ever sent is worth almost $30,000. On Nov. 24, 2001, a Christmas card was sold at auction in Devizes, Wiltshire, U.K., for £20,000, or $28,158, making it the most valuable Christmas card in the world. That's because it was "considered the world's first Christmas card," according to Guinness World Records, which notes that it "was sent by Sir Henry Cole, a Bath-born businessman, to his grandmother in 1843 and is hand-colored by the London illustrator John Calcott Horsley." The lithographed illustration depicts a multigenerational family enjoying a Christmas party and there are only 12 of the original 1,000 cards still in existence. These days, over a billion and a half Christmas cards are sent each year in the U.S. These days, around 1.6 billion people in the U.S. still manage to send out the old-fashioned greetings each year. According to data from Hallmark via The Atlantic, "Millennials represent nearly 20 percent of the dollars spent on greeting cards, and their spending is growing faster than that of any other generation." And the smallest Christmas card in the world is invisible to the human eye. Most Christmas cards are big enough for a seasonal illustration or photo as well as space inside for a heartfelt message. But in 2010, nanotechnologists at the University of Glasgow created a teeny-tiny Christmas card that could fit onto the surface of a postage stamp 8,276 times or onto a regular-sized Christmas card around half a million times. While the card is invisible to the human eye, Professor David Cumming discussed producing the microscopic greeting card with Time, saying, "The process to manufacture the card only took 30 minutes. It was very straightforward to produce as the process is highly repeatable. The design of the card took far longer than the production." Christmas Day boasts the lowest number of relationship breakups. The holidays aren't always easy on romantic relationships, but if you and your significant other make it to Christmas Day, you can probably take the day off from worrying that you're going to break up. A 2010 study conducted by Facebook found that Dec. 25 had the fewest number of breakups. The weeks leading up to Christmas on the other hand? Yikes! And it's the most popular time of year to propose. According to The Washington Post, wedding experts and social media data indicate that Christmas Eve and Christmas Day are the two most popular days of the year to propose. In addition, the wedding app Bridebook also estimated that around 100,000 proposals were likely to take place during the Christmas season in 2018, as Harper's Bazaar reported. A record number of babies is conceived around Christmas. Studies have shown that September is the most popular time for babies to be born in the U.S. Check the calendar and that means that those little ones were conceived around the holiday season when couples are feeling rather, er, cozy. The largest gingerbread house ever made was as big as a real house. If you think that putting together a regular-sized gingerbread house is tricky, try making one that's big enough to walk into. In November 2013, a group from Traditions Club in Bryan, Texas, constructed a gingerbread house that was 160 feet long, 42 feet wide, and 10.1 feet tall. Made with a wood base, it reportedly took 1,800 pounds of butter, 7,200 eggs, 3,000 pounds of sugar, 7,200 pounds of flour, and over 22,000 pieces of candy to cover the record-breaking structure! RELATED: 15 Royal Christmas Traditions That You Need to Know About. And the largest snowflake ornament ever was over 10 feet tall. According to Guinness World Records, the largest Christmas snowflake ornament ever was created by Universal Studios Japan in October 2019. It stood an incredible 10 feet and five inches tall, truly a tree topper to top all tree toppers. The tree it was placed upon also earned a Guinness title for the most lights on an artificial Christmas tree: a whopping 591,840. Americans spend nearly $2 billion on holiday treats every year. It used to be just candy canes and gingerbread. But these days, the Christmas sweets industry is booming. "It's candy-cane ice cream, candy-cane lattes, candy-cane marshmallows," Christine Couvelier, president of the food development firm Culinary Concierge, explained to USA Today in 2017. And boy, do we buy into it: Americans spend an estimated $1.93 billion on holiday treats, according to the National Confectioners Association. There are nearly 750 different versions of "Silent Night." "Silent Night" has long been a staple of the holiday season, when the hauntingly beautiful carol can be heard almost everywhere you go. So it makes sense that it is, in fact, the most recorded Christmas song in history. According to Time, as of 2014, there had been over 733 different versions of "Silent Night" copyrighted since 1978—and more continue to pop up every year! Home Alone is the highest-grossing Christmas movie of all time. When Macaulay Culkin starred as a young boy accidentally abandoned by his family during the holidays in 1990's Home Alone, the child actor became an instant star and the film an instant Christmas classic. According to Forbes, Home Alone remains the highest-grossing Christmas movie of all time in the U.S., earning $285.76 million at the domestic box office. It's followed by 2000's How the Grinch Stole Christmas ($260.04 million), 2018's Dr. Seuss' The Grinch ($189.67 million), and 2004's The Polar Express ($183.37 million). Decorating Disney World involves 8.5 million lights. While many people head to Disney World in the summer, others like to visit the most magical place on Earth during the most magical time of year. And to prepare for the festivities, Disney embarks upon a remarkable setup. According to Trip Savvy, preparing for the holidays Disney-style involves "unloading 150 semi-trailer truckloads of decorations, stringing 15 miles of garland and 8.5 million lights, hanging 1,314 wreaths, and trimming some 1,300 trees with 300,000 yards of ribbon and bows across Disney World's four theme parks, two water parks, and over two dozen Disney World resort hotels." The tradition of putting up Christmas trees is more than 500 years old. Putting up a Christmas tree has been a part of American holidays for years. However, it's a tradition that can be traced back to Germany. According to History, "Germany is credited with starting the Christmas tree tradition as we now know it in the 16th century when devout Christians brought decorated trees into their homes." The custom didn't come to the U.S. until the late 1700s or early 1800s, via German settlers in Pennsylvania. At first, Christmas trees weren't accepted in the U.S. Christmas trees weren't an immediate hit in America when they were first introduced to the U.S. by German settlers due to the fact that they weren't seen as Christian. "As late as the 1840s, Christmas trees were seen as pagan symbols and not accepted by most Americans," according to History. There are more than 630 different kinds of Christmas trees. When you go with a real Christmas tree over an artificial one, you have more than a few different options to choose from. For example, there are about 630 species of conifer trees alone, including popular choices like balsam fir, Douglas fir, and Fraser fir. And the U.S. grows 77 million of them annually. According to History, more than 1 million acres of American soil have been planted with Christmas trees. And at about 2,000 trees per acre, that adds up to around 77 million Christmas trees planted in the U.S. each year! In fact, they're grown in all 50 states—yes, even Hawaii! No matter where you live in the U.S., you can find a homegrown Christmas tree, likely from a nearby farm. That's because Christmas trees are grown in all 50 states, including Hawaii and Alaska, according to History. The biggest elf gathering ever included over 1,700 of Santa's elves. You might think that Santa's little helpers would be busy making toys for children all year long. But on Nov. 25, 2014, 1,762 elves came together in Bangkok, Thailand, to set the record for the largest gathering of Santa's elves ever. But one of the biggest stateside gatherings is Elfapalooza in Alabama. The people of Mobile, Alabama, are apparently determined to beat the record for the largest gathering of Santa's elves. That's why American elves meet up at the annual Elfapalooza event, which includes karaoke, best elf contests, and an elf march! Stockbridge, Massachusetts, recreates a Norman Rockwell Christmas painting each year. Stan Tess / Alamy Stock Photo The picturesque town of Stockbridge, Massachusetts, becomes even more picturesque during the holidays when it transforms into a Norman Rockwell painting. Each December, according to the town's website, Stockbridge recreates the Sunday scene depicted in Rockwell's 1967 work, Stockbridge Main Street at Christmas (Home for Christmas), complete with vintage automobiles parked in the spots occupied in the painting. It's pretty much the most wholesome, dare we say Rockwellian, example of American culture we could ever imagine! Arizona has a 30-foot Christmas tree made of tumbleweeds. There are real Christmas trees, artificial Christmas trees, and in Chandler, Arizona, there's a 30-foot Christmas tree made out of tumbleweeds. It takes 1,200 tumbleweeds to create the structure, which is then covered in glitter as well as 20 gallons of flame retardant, according to Fodor's Travel. You can recycle your Christmas tree by donating it to elephants. When the holidays are over and it's time to get rid of your Christmas tree, you could drag it to the curb to be picked up by garbage collectors or you could donate it to a zoo so that it can be fed to a hungry elephant. Zoos around the world, including The Elephant Sanctuary in Hohenwald, Tennessee, accept evergreens, which are enjoyed by the giant herbivores as a seasonal snack. Mistletoe translates to "dung on a stick." When you find yourself standing under a sprig of mistletoe, it's time for a Christmas kiss. But, while mistletoe was once considered an aphrodisiac by the Druids, it also happens to mean "dung on a stick." According to Interflora, the original name for mistletoe was mistaltan—mistal comes from the Anglo Saxon word for "dung" and tan means "stick." How utterly unromantic! Poinsettias have been a symbol of Christmas for over a century. These beautiful red and green flowering plants have been associated with Christmas for over 100 years. Back in 1828, the American minister to Mexico, Joel R. Poinsett, brought a poinsettia home to America from his post down south. Getting their name from Poinsett, the plants gained popularity in the U.S. after stores in New York began to sell them at Christmas in 1870 and by the 20th century, "they were a universal symbol of the holiday," according to History. Santa has more than 30 different names. Did you know that Santa Claus is known by a number of festive names around the world? In Hungary, for instance, he goes by Mikulás, while those in Brazil refer to him as Papai Noel. He'll also answer to Hoteiosho in Japan, Julenisse in Norway, and Père Noël in France. And those are just a few of Kris Kringle's 30-plus international monikers. You can visit Santa's village all year round in Canada. Santa's Village is a festive theme park that's open 365 days a year in Canada, so you can visit during the winter months or camp there during the summer. Many movies have also been filmed at the seasonal location, including Hallmark's Christmas in Grand Valley and Netflix's The Knight Before Christmas starring Vanessa Hudgens. Santa performers can make up to $1,200 an hour. Santa Claus may be a one-of-a-kind Christmas character, but plenty of performers dress up as the festive figure each year to make extra money. During the holidays, mall Santas earn around $30 an hour but can make up to $100 per hour for their work. However, Dan Greenleaf, a professional freelance Santa who runs his own booking agency, told CNBC that when he's hired to visit parties, homes, or other events, the "typical hourly mid-range is about $150." And if you're really good at delivering a jolly "ho, ho, ho," then you can potentially make $500 an hour. Greenleaf even says one of the Santas who works for him was offered $1,200 by a Manhattan client for just one hour on Christmas Day! Around 28 Lego sets are sold every second during the Christmas season. If there's a child in your life, then there's a good chance that they've asked for at least one Lego set as a gift over the years. In fact, they probably ask for one each year. That's why, during the Christmas season, almost 28 Lego sets are sold each and every second, according to National Geographic Kids. The day after Christmas is National Candy Cane Day. Dec. 26 isn't just Boxing Day—it's also National Candy Cane Day, "and while that doesn't mean it'll keep us from munching on the sugary sticks as early as Thanksgiving, it does give us a chance to indulge as much as we can before New Year's," notes National Today. People in Japan eat KFC on Christmas. In America, families who celebrate Christmas might enjoy a turkey or ham for their holiday dinner. But over in Japan, modern holiday festivities often involve eating KFC at Christmas. According to the BBC, it's estimated that 3.6 million Japanese families enjoy the fast-food chicken on Dec. 25. The credit goes to Takeshi Okawara, the manager of the first KFC in the country, who overhead a couple of foreigners talking in his store after it opened in 1970 about missing turkey on Christmas. "Okawara woke up at midnight and jotted down an idea that came to him in a dream: a 'party barrel' to be sold on Christmas," according to the BBC. He "hoped a Christmas dinner of fried chicken could be a fine substitute, and so he began marketing his party barrel as a way to celebrate the holiday." The man who wrote The Legend of Sleepy Hollow is credited with creating the modern view of Christmas. While he is most famously associated with Halloween thanks to his creepy classic, The Legend of Sleepy Hollow, Washington Irving was actually partially responsible for shaping the Christmas traditions we know and love today. "Among Irving's biggest contributions to Christmas in America was his promotion of St. Nicholas as a beloved character, laying the groundwork for the figure we'd eventually embrace as Santa Claus," the National Endowment for the Humanities notes. The yule log calls back to a holiday tradition from the Iron Age. etorres/Shutterstock According to History, that log-shaped, chocolate-y dessert that you present to your guests every year has its roots in Gaelic and Celtic Winter Solstice traditions. "To cleanse the air of the previous year's events and to usher in the spring, families would burn logs decorated with holly, pinecones or ivy," the site states. "Wine and salt were also often used to anoint the logs. Once burned, the log's ashes were valuable treasures said to have medicinal benefits and to guard against evil." Over the centuries, the good-luck ritual developed into this tasty holiday treat. A handwritten copy of 'Twas the Night Before Christmas was sold for $280,000. Victorian Traditions/Shutterstock In 2006, an unnamed business executive purchased one of four known handwritten copies of Clement Clarke Moore's poem, A Visit From Saint Nicholas, which is better known now as 'Twas the Night Before Christmas, at auction. The 1860 poem, also signed by the author, went for $280,000 and was reportedly read out loud by the buyer at a holiday party. Mariah Carey wrote "All I Want for Christmas Is You" in 15 minutes. YouTube/Mariah Carey Yep, that's right. It took Mariah Carey less than half an hour to co-write her now-ubiquitous Christmas pop song. Her collaborator Walter Afanasieff told Billboard, "That's why it's so popular—because it's so simple and palatable!" Children have been leaving cookies and milk out for Santa since at least 1896. Hurst Photo/Shutterstock No one knows exactly why it's now customary to leave out cookies and milk for Santa Claus, but, per NPR, the first recorded reference to the tradition is from a Santa-themed magazine for children that was published in the late 19th century. A young girl wrote to St. Nicholas: An Illustrated Magazine for Young Folks that she and her brother were planning to leave the snack out for their Christmas visitor in case he was hungry. Tens of thousands of people go to the ER each year with decorating-related injuries. TayaJohnston/Shutterstock Make sure you have a spot when you're stringing those lights! According to the United States Consumer Product Safety Commission, in 2018, 17,500 people sought treatment at a hospital for injuries sustained while decorating for the holiday. Santa Claus wasn't always partial to the color red. Kiselev Andrey Valerevich/Shutterstock These days, Santa is almost always depicted as a jolly, bearded man in a bright red suit with furry white trim. But this color scheme is a surprisingly recent development. Prior to the 1870s, Santa was shown wearing a range of colors, including brown, white, green, tan, and blue. Per The Guardian, Thomas Nast, a Harpers Bazaar caricaturist, is credited for originating the red-suited version of Santa we know so well today. Even though he himself had also drawn him in other hues, this is the one that stuck. RELATED: The Real Reason Harry & Meghan Won't Be at the Royal Christmas, Sources Say. Desirée O Desirée O is a freelance writer who covers lifestyle, food, and nutrition news among other topics. Read more Christmas • Facts • Holidays • Trivia The 5 Best Times to Shop at Bath & Body Works This is precisely when you should be re-upping on your favorite creams and candles. The Bath & Body Works Scent You Should Use Are you more of a seasonal scent or a classic pick? Floral or woodsy? Let the stars decide. Celebs Who Vow to Never Get Cosmetic Surgery These stars plan on aging gracefully—and realistically. 5 Things Never To Say During an Argument It will only make it worse. 6 Best Hairstyles for Growing Out Your Grays They make blending a breeze. 5 Tips to Become a Pickleball Pro This Year Experts say these will help you up your game. 6 Secrets Kroger Doesn't Want You to Know Read this before your next supermarket trip. How To Get Back Items Confiscated by TSA They're not gone forever. Miley Cyrus "Was Chased Down" by a UFO At least, she thinks that's what she saw. © 2022 Galvanized Media. All Rights Reserved. Bestlifeonline.com is part of the Meredith Health Group

my Switch friend code: SW-7893-6421-8896 – Did You Win Yet? Nintendo is still using Friend Codes? They really don't get online connectivity, still. All they need to do is copy Steam or XBL wholesale, and everyone would be happy.

Q: Value assignment for complete, transitive relation on uncountably infinite set Consider a set $A$ and a relation $r$. The relation $r$ is complete, i.e., for any $a,b\in A$, we have $arb$ or $bra$ or both. The relation $r$ is transitive, i.e., for any $a,b,c\in A$, if $arb$ and $brc$, then $arc$. Must there exist a function $f:A\rightarrow\mathbb{R}$ such that for any $a,b\in A$, we have $arb$ if and only if $f(a)\geq f(b)$? If $A$ is countably infinite, the answer is yes. I'm wondering what about if $A$ is uncountably infinite? I'm almost sure there must be a counterexample, but I don't know how to find one. (For example, if $A=\mathbb{R}$, then the trivial map $f(x)=x$ works.) A: The answer is no. What you asking is basically, whether $A/{\sim}$ can be embedded into $\langle\mathbb{R},\leq\rangle$, where $$x \sim y \quad\text{ if and only if }\quad x\ r\ y \land y\ r\ x.$$ A counterexample might be $\langle\mathbb{R}^2,\leq_\text{lex}\rangle$, that is, $$(x_1,y_1) \leq_\text{lex} (x_2,y_2) \quad\text{ if and only if }\quad x_1 < x_2 \lor (x_1 = x_2 \land y_1 \leq y_2).$$ Here $\sim$ would be just identity, so we are trying to map lexicographical order on $\mathbb{R}^2$ into standard order of $\mathbb{R}$. Why these two are different? Suppose there exists an order-preserving mapping $f : \mathbb{R}^2 \to \mathbb{R}$ and define \begin{align} a_x &= f(x,0) \\ b_x &= f(x,1) \end{align} and observe that $a_x \lneq_\text{lex} b_x$ and for $x < y$ also $b_x \lneq_\text{lex} a_y$. In other words $\{(a_x,b_x) \mid x \in \mathbb{R}\}$ would constitute an uncountable set of disjoint open intervals, which is impossible (e.g. because rational numbers are countable). I hope this helps $\ddot\smile$

Atarba ignithorax är en tvåvingeart som först beskrevs av Alexander 1929. Atarba ignithorax ingår i släktet Atarba och familjen småharkrankar. Inga underarter finns listade i Catalogue of Life. Källor Småharkrankar ignithorax

Baltimore, Maryland Litigation Lawyers Silverman Thompson Home Contact Pikesville, Maryland Appellate Lawyers Silverman Thompson STSW Client Lakeview Title Again Vindicated on Appeal Over the past eight-plus years, litigation has been pending against co-defendants Long & Foster/Creig Northrop and STSW client Lakeview Title in Maryland's state and federal courts for supposed kickbacks paid by Lakeview to Long & Foster. The state court action, styled Larocca v. Creig Northrop Team, P.C., et al., resulted in the trial court striking an amended complaint against Lakeview and its owner, Lin Eagan, a decision upheld by the Court of Special Appeals. 217 Md. App. 536 (2014). The federal matter, styled Baehr v. the Creig Northrop Team, P.C., traveled a longer and more circuitous route, with retired Judge Quarles certifying a class early in the matter only to have Judge Bennett dismiss the matter at summary judgment. In a unanimous, published opinion dated March 13, 2020, the United States Court of Appeals for the Fourth Circuit affirmed that decision, finding the plaintiffs lacked standing to sue and thus dismissal of the case was warranted. Baehr v. The Creig Northrop Team, P.C., et al., -- F.3d - (4th Cir. Mar. 13, 2020). STSW attorneys Andy White and Bill Sinclair litigated both matters on behalf of Lakeview Title. STSW Client Lakeview Title Again Vindicated on Appeal | Baltimore, Maryland Litigation Lawyers

This is a priceless picture of my snorkie name "Jazzi" it was a Christmas gift from my Mother. In regard to your 1/23/13 post about what lace type---it is Teneriffe Lace. It is originally from the Canary Islands (Tenerife being the largest island) but the lace type has spread to Latin America--Central and South America.

Use the mouse to place down blocks. The blocks on the right show you what's coming next. Over time, poetry appears and the rules of the game change. By placing down blocks in certain patterns, you try to make the ones shown on the left. Complete all 6 to find the good ending. EXTREMELY creative game with just enough curveballs in the mechanics to keep me engaged. Well done. Good game, did not get the point of it though, but I got that it was about a love story. Gorgeous, fun game. Great job! Very creative, more contrasted hues could make the game easier. Nice idea. Kind of challenging to keep track of the 6 patterns (especially when second world appears), but still, neat game. This is Creative and interesting ! GREAT WORK ! Took me two attempts to beat, has a good difficulty for an LD-game. To improve replayability you could make the patterns on the left randomized. Very interesting game. It was very confusing at first but I managed to get all 6 patterns. Awesome concept. Good puzzle game with a nice aesthetic and surrounding biz. Cool. Took me quite a while but I finished it successfully eventually. Really relaxing game, even though I am horrible at it. Didn't get the point either. I love the color scheme and music, but I think using premade music is against the 48h compo rules :/. Great game, my only complaint is the low variation in the blue tones. Lines of poetry as levels - that's a very interesting idea. I found it hard to see the specific colours, and I had to move very slowly and carefully to understand whether I could match anything. Good, engaging game – I played until the end (although I didn't *quite* make it to the happy ending). Very nice look, and nice concept, but really hard to get my head around this puzzle. I didn't manage to get the good ending. I got too frustrated with the mechanics as they were new to me. Hi there. Interesting puzzle, liked the peaceful sea sounds. Found the puzzle itself very much like office work. I couldn't remember the all targets so referring back and forth the whole time, which broke my engagement with it.

CT Dems hold watch party at Hartford baseball stadium Electric vehicles steal the spotlight at 2022 Houston Auto Show Aug. 20, 2020 Updated: Aug. 20, 2020 11:59 p.m. Congresswoman Rosa DeLauro speaks to the media during the virtual Democratic Convention for the nomination of Joe Biden for president at Dunkin' Donuts Park in Hartford in August. Christian Abraham / Hearst Connecticut MediaShow MoreShow Less Sen. Richard Blumenthal speaks to the media during the virtual Democratic convention for the nomination of Joe Biden for president at Dunkin' Donuts Park in Hartford on Aug. 20. Christian Abraham / Hearst CT MediaShow MoreShow Less Congresswoman Rosa DeLauro, left, chats with Senator Chris Murphy as they attend the virtual democratic convention for the nomination of Joe Biden for president at Dunkin' Donuts Park in Hartford on Thursday. US Senator Richard Blumenthal speaks to the media during the virtual democratic convention for the nomination of Joe Biden for president at Dunkin' Donuts Park in Hartford, Conn., on Thursday Aug. 20, 2020. Democratic Party Chairman Nancy DiNardo speaks to the media while attending the virtual democratic convention for the nomination of Joe Biden for president at Dunkin' Donuts Park in Hartford, Conn., on Thursday Aug. 20, 2020. Delegate Audrey Blondin, of Goshen, attends the virtual democratic convention for the nomination of Joe Biden for president at Dunkin' Donuts Park in Hartford, Conn., on Thursday Aug. 20, 2020. Dunkin' Donuts Park is the site for the state's virtual democratic convention for the nomination of Joe Biden for president in Hartford, Conn., on Thursday Aug. 20, 2020. Senator Chris Murphy, right, chats with Congresswoman Rosa DeLauro as the attend the virtual democratic convention for the nomination of Joe Biden for president at Dunkin' Donuts Park in Hartford, Conn., on Thursday Aug. 20, 2020. Nancy DiNardo, chairman of the Connecticut Democratic Party Tyler Sizemore / Tyler SizemoreShow MoreShow Less In a 2019 file photo, Nancy Wyman, then-Democratic state chairwoman, left, joined Speaker of the U.S. House Nancy Pelosi, center, and Nancy DiNardo, right, who has since returned to the head the Democratic State Central Committee. Dan Haar /Hearst Connecticut Media /Show MoreShow Less Democratic Party Chairman Nancy DiNardo US Senator Chris Murphy speaks to the media during the virtual democratic convention for the nomination of Joe Biden for president at Dunkin' Donuts Park in Hartford, Conn., on Thursday Aug. 20, 2020. Hartford Mayor Luke Bronin speaks to the media during the virtual democratic convention for the nomination of Joe Biden for president at Dunkin' Donuts Park in Hartford, Conn., on Thursday Aug. 20, 2020. Participants sit on the field during the virtual democratic convention awaiting the nomination of Joe Biden for president at Dunkin' Donuts Park in Hartford, Conn., on Thursday Aug. 20, 2020. Governor Ned Lamont attends the democratic convention for the nomination of Joe Biden for US President at Dunkin' Donuts Park in Hartford, Conn., on Thursday Aug. 20, 2020. Former Vice-president Joe Biden accepts the nomination for US President during the virtual democratic convention for Connecticut Democrats at Dunkin' Donuts Park in Hartford, Conn., on Thursday Aug. 20, 2020. HARTFORD — State Democrats turned a minor-league baseball stadium into a political rally Thursday to celebrate the nomination of former Vice President Joe Biden in a virtual Democratic National Convention watch party that is the prelude to a historic November showdown for the White House. By the time of the broadcast of Biden's acceptance speech on the big screen in left field, only about half of the state's 100 delegtes were left at the tables spred around the infield. And their applause when Biden declared that if elected president, he would "restore the promise of America," was nothing close to the roar of a Yard Goats crowd. But the coronavirus pandemic cancelled the AA baseball season, and the convention in Milwaukee, for that matter, but the state party wanted to do something to bring people together and to give delegates a perk to energize them into the fall campaign. "It was very moving," said delegate Lydia Martinez of Bridgeport as she walked toward the exit at about 11:30. Delegates would have otherwise been whooping it up in Milwaukee, but rather than watch the virtual event at home, they accepted the limits of political spectacles in the age of the coronavirus and, as they have all week, watched from afar a big screen TV package of music, platform videos and high-profile pols, including the roster of presidential hopefuls tht Biden overcame on his way to the nomination. The evening was divided into two parts: a cocktail party for Connecticut's highest elected officils, and the watch party for the rank-and-file delegates in a balancing act of masks, limited attendance and restrictions on reporter access. "The days of cozying up to foreign dictators are over," Biden said as the political operatives clapped and cheered from their infield tables. "This is our moment to make hope and history rhyme." After the cocktil event, and early enough for most TV and newspaper deadlines, U.S. Sen. Richard Blumenthal, of Greenwich, said the week's virtual convention has been "electrifying" and underscores the differences between Biden and President Donald Trump, while being entirely different from noisy halls and long speeches. "This one has been so relatable and moving," Blumenthal told reporters. "Donald Trump has killed Americans through his negligence, his contempt for the rule of law and science, and that is coming across so powerfully and convincingly. The only way Donald Trump wins this election is to steal it. To sabotage the election machinery, to diminishing and degrading the Post Office, but also inviting the Russians to continue interfering." Blumenthal said that the top-secret documents on Russians interference indicates that the mischief is continuing. Gov. Ned Lamont, an early Biden supporter, said the candidate appreciates the help from state residents. "Everybody trusts the man, understands the man," Lamont told reporters. "He's been around for a long time. This is a guy everybody knows well, and he knows them. I'm 66 years old, I have never seen such a consequential election in my life. Another four years of Donald Trump? That might be irreversible." Attorney General William Tong of Stamford, who sponsored the cocktail reception before the evening's viewing, charged that Trump's overt attempt to slow down the U.S. Postal Service, with his postmaster general ripping out huge mail-processing machines in mail distribution centers throughout the country, is upsetting. 3rd District U.S. Rep. Rosa DeLauro of New Haven said the fundamental issue in the upcoming presidential will be "trust." But the Trump administration has split the country while "hollowing out" federal service agencies, she said. "What they did, deliberately in my view, with this pandemic and the coronavirus, is to further erode that trust in government," she said. "When people's health is at grave risk, their economic security is at grave risk, their social justice is at grave risk, and that we know in these areas and what this pandemic exposed was the racial inequities in all these areas." Lt. Gov. Susan Bysiewicz, who earlier in the week participated in a panel discussion on the 100th anniversary of the 19th Amendment, said Kamala Harris's choice as a running mate is something she will cherish forever. "Knowing that this is the week we are celebrating the 100th anniversary of women's suffrage, to have Kamala Harris chosen to be the vice presidential candidate for a major party is historic, and I've just been enjoying this moment all week," she said. "We don't have a human being in the White House today," said U.S. Sen. Chris Murphy. "We don't have somebody that cares at all about what families are going through. We don't have somebody in the White House who grieves when 170,000 Americans have died and 70 million have lost their jobs." The night culminated with the high-stakes, prime time acceptance speech of Biden, who faces the challenge of framing himself to uncommitted voters as an alternative to the Republican president, whom he attacked as unprepared for a job he hasn't grown into, with his only success dividing the country. For State Party Chairman Nancy DiNardo of Trumbull, the virtual convention marked a 48-year association with national Democratic politics, going back to 1972, when U.S. Sen. George McGovern, who was crushed in a landslide by then-President Richard Nixon. That year, in New York's Madison Square Garden, DiNardo checked credentials at one of the building's entrances. DiNardo's first stint as state party leader stretched from 2005 to 2015. After the 2018 election, Nancy Wyman, the former lieutenant governor, became the public face of the party for a couple years. Then after Wyman retired earlier in 2020, Lamont asked DiNardo to return. "The governor knew I have experience with both state and national conventions, not that that matters this year," DiNardo quipped on Thursday. "The staff did a great job putting this together." The four-day schedule has included daily virtual "breakfasts," with guests such as Lamont, members of the congressional delegation and former presidential hopeful Beto O'Rourke. The ball park was rented for $18,000. As of Thursday, state Republicans, whose national convention to nominate the president for a second term, hve not decided on a spot for a watch party. Thursday's breakfast speaker was Chris Dodd, the former longtime U.S. senator who co-authored massive changes to the oversight of the nation's financial industry after the real estate bubble burst in 2008, sparking the Great Recession. In the afternoon, state Democrats released a video that featured Connecticut actors James Naughton, Harvey Fierstein and musician Javier Colon, along with others who — in six words, say why they support Biden and running mate Harris. "We weren't sure what we'd get with the six-word challenge, but what people shared was just great," DiNardo said. Others participating in the home-video project were television producer Jody Hamilton, daughter of Carol Burnett, Bysiewicz, U.S. Rep. Jahana Hayes, Mashantucket Pequot Chairman Rodney Butler, state Sen. Saud Anwar; former Bloomfield Mayor Joseph Suggs, Connecticut Public producer Harriet Unger, student Izzie Schackner, actress and dancer Carolyn Paine; dancer Alex Zarlengo; registered nurse Madeline Appleton, Deputy Consumer Protection Commissioner Arunan Arulampalam and his family, and South Windsor Mayor Andrew Paterna.

Review: Woods' With Light & With Love - And A Lesson In Sharing By Tarik Moody Published April 30, 2014 at 1:00 AM CDT Looking back on my adolescence I can still distinctly recall being 12 years old and that feeling of individual accomplishment, so rare at that age, when I discovered a film, book or piece of music that I felt none of my peers had yet discovered. Finding art that speaks to your individual condition is, and always will be worthy of amazement, but when your perception of the world is cascading all around you, those things that you cling to are all the more significant, purely because they are the only things you can be certain of. I remember hording every album that I found at that age, even painstakingly hiding the albums when friends would come over, terrified of the possibility of someone relating to one of the only things I was certain related to me. That all changed in the summer of 2003 when my buddy Joe invited me over to his house and made me privy to the Stills' Logic Will Break Your Heart, which despite being critically well received, seemed like an intimate confession of profound friendship on Joe's part. From that day forward I resolved never to hide good music from anybody. Yet, when 2009's incredible Songs Of Shame came out I couldn't help but feel as if I was hording Woods. Unlike my pubescent self I wasn't doing so intentionally and despite the album's relative acclaim, I couldn't help but feel like no one was paying attention to Woods but me. Fast forward 5 years, 3 albums, numerous 45's and countless under-attended shows to today, two weeks after the release of their 7 th album, With Light & With Love and I still can't help but feel like they are the best kept secret in music. Like every album in Woods' catalogue With Light & With Love is an exploration in aesthetic and an evolution in chemistry between songwriter Jeremy Earl and guitarist/producer Jarvis Taveniere. Since Songs Of Shame the band has gradually navigated translating their scuzzy, psychedelic live jams onto wax without compromising their folk sensibilities. With Light & With Love is their most well balanced alchemy to date (which is something I feel like I say after every release); their sound evokes their americana and experimental influences in ways they seemed timid to fully embrace in the past. Whether it be the newly texturally employed organ, reminiscent of The Bandon "Leaves Like Glass" or the honky-tonk slide-guitar akin to the Flying Burrito Brothers on "Full Moon" Woods manage to seamlessly infuse their sound with newfound layers that never distract from their distinct sonic amalgam. While much of this progression is natural, with Earl, Taveniere and bassist Kevin Morby having played together extensively for the past few years, a lot of praise should be directed to Taveniere's evolution as a sound engineer and producer. Undoubtably much of Woods' shifts in sonic quality from album to album were intentional, but upon reviewing their sound from each album leading up to With Light & With Love it would seem that Taveniere (as a producer) was building toward this all along. It's worth mentioning that Taveniere also produced last years overlooked, self-titled album from Happy Jawbone Family Band and Quilt's excellent new record Held In Splendor . When listening to Woods it has always been the case that Jeremy Earl's fragile falsetto does more to obscure his lyrics than amplify them, With Light & With Love being no different. There is a sort of beautiful, understated brilliance though to having a songwriter whose songwriting is often obfuscated by the bands lush instrumentation; Earl somehow remains the antithesis of a conventional frontman while consistently pitching remarkably poetic and profound mantras worthy of their own published collection. Since I discovered Woods I've had to weigh the positives and negatives of having a favorite band that I felt many were missing out on. Part of me was delighted to once again have something that so profoundly spoke to my condition, seemingly all to myself (turns out it's still hard to know who you are). But the other part of me felt guilty that a band that embodies all the beauty of music was being so unjustifiably overlooked. A few years back I was fortunate enough to turn Joe (same one) onto Woods, since which we have seen them a few times together and are actually headed to a one day festival in California this summer that Woods is hosting. The lesson being that a shared love is always more meaningful than one kept to yourself, even if it risks exposing a secret aspect of yourself. Woods will play with Quilt at Subterranean in Chicago on May 7th, tickets HERE With Light & With Love is available at Woodsist, the label run by Jeremy Earl. It is uncoincidentally one of the best labels around. Tarik Moody HYFIN Program Director | Radio Milwaukee See stories by Tarik Moody

The Congress of the People, having taken cognizance of the urgent need for the transformation of our historically neglected townships, the spread of unplanned informal settlements without services and the failure to provide quality services to the residents of our towns and cities calls on patriotic South Africans with a passion and commitment to serve, to step forward. COPE invites men and women who genuinely wish to serve their communities with passion and capability to declare their availability to serve our people in the sphere of local government. COPE has now opened the process to identify prospective councillor-candidates to contest the 2016 Municipal Elections under its banners. This will be a watershed election and we need candidates who can rise to the steepest challenge our nation faces. Our candidates must be people who are honest and committed to serving the people, dedicated to protecting and preserving public resources and assets and determined to ensure that corruption, waste and mismanagement are eradicated. COPE implicitly believes in accountability. We therefore insist that our public representatives will continuously subject themselves and their actions (or lack of action) to sharp and consistent scrutiny. Accountability ensures quality outcomes and we therefore insist on our public representatives being accountable. continuously seek to refresh their mandates from their constituencies and not purport to be working under instructions from called party leaders who are far and remote from the real challenges facing their particular communities and people on a daily basis. In 2009 we said that we were "inspired by the vision shared by all our people, black and white, of the sense of history as we get a new opportunity to build a new people-centered, caring, inclusive and common South African nation". We still believe that goal is possible. This time we recognize the extreme urgency in achieving that objective. We also know for certain that the vast majority of people in our country will agree that "South Africa deserves a BETTER GOVERNMENT. They will also agree that South Africans need a government that is RELIABLE, ACCOUNTABLE AND INCORRUPTIBLE". This is particularly urgent at the local sphere of government because that is the coal face where people and their representatives interact daily. The men and women we invite to be part of this new crusade must be clear in their conscience and their hearts of the need to enter into an unbreakable contract with the people, to serve them with humility and uncompromising commitment. We do not need career politicians. We are confident that the journey we are embarking upon will yield positive results. We are indeed determined to attract capable men and women who will consistently and unswervingly be trustworthy and will who serve the people with passion and respect. We believe that our country is full of good, hard-working people. We also believe that we have amongst us those will not tolerate corruption and personal greed. The dominance of one political party and its closed nature of selecting candidates has robbed the nation of quality candidates. This has also caused the boundary between state and the party to become blurred. Our democracy is being seriously damaged by sycophants and hangers on who forsake accountability to feather their own nest. The time has come to reject those who continuously defer to their party bosses in order to pluck the fruits of patronage that they hang out. We want candidates who will stand with their communities. We want candidates who will take the pleas of the Auditor General to heart and stop futile and fruitless expenditure. We are encouraging people, first and foremost, to stand in their own individual right as ward candidates wherever they feel they have the support of the residents. Let that be the bond that cannot be broken. For change to come and for transformation to occur, it is individuals with commitment, capability and vision who will need to take up the challenge. There is no requirement for anyone to be a member of COPE first before declaring his / her availability to serve. The big requirement is who the community trusts and has confidence in. Individuals who meet the criteria for candidates and accept to conform with the electoral guidelines that the party subscribes to, will require to become members and be bound by COPE's constitution and policies. We, however, are committed to ensuring that, even when a councillor becomes a member of the party, he / she will unconditionally maintain the distinction and separation between party and state and remain fully accountable to the given electorate. Where we believe that an independent candidate has better prospects of winning the vote of the majority of residents of the ward, we shall lend our support instead of fielding a candidate. The interests of the residents come first. The Congress of the People has gained valuable experience in forging common interests among South Africans who share the same values and principles where appropriate. We believe that there is more that unites us than that which divides our people, black and white. We are ready, and will continue to work with other like-minded opposition parties by agreement. We are committed to building a future in which the arrogance of power will not imperil good governance and hold genuine democracy hostage. THIS IS A CALL TO SERVE WITH DISTINCTION!

Ernest Jones launches Pandora charm bracelets, necklaces and anklets which enable the creation of exciting mixtures of beads and charms to celebrate an event or an occasion. PR9.NET November 21, 2008 - Pandora charms are now available at Ernest Jones. Personal, fun and fashionable, Pandora jewellery can be used to celebrate any occasion from birthdays to a special day with a loved one, given as a memento, gifts, or for collecting charms to make personal bracelets, necklaces or anklets. The Pandora range at Ernest Jones offers affordable, fashionable and exciting jewellery for all tastes. Pandora's designers seek to inspire and be inspired by all women interested in creating their own style and telling their own unique story. Pandora believes that a piece of jewellery is a woman's signature. As a result they have created a large selection of matching jewellery in both gold and silver, so women can match their mood and style, whether it is romantic and dreamy, casual jeans with a touch of elegance or an evening gown and high heels. The new collection from Ernest Jones makes creating a Pandora charm bracelet (http://www.ernestjones.co.uk/webstore/browse/N/179+4294966708/ ) or necklace easy. Customers simply choose which length and material they would like from a range of 14ct gold or silver chains with lengths of 19cm to 45cm, providing a variety of uses as either necklaces or bracelets. Once the chain is chosen Pandora also offer a wide range of clasps and safety chains to choose from. Next customers choose the beads that they want. With a wide selection ranging from simple beads to skulls, brightly coloured charms, silver, gold, Swarovksi crystal, birthstones, a beautifully decorated cake for a child's first birthday, a plump heart for a girlfriend or boyfriend, a suitcase for an exotic holiday, the Pandora charm (http://www.ernestjones.co.uk/webstore/browse/N/58+4294966708/ ) and bead collection has something for every occasion. Pandora jewellery (http://www.ernestjones.co.uk/webstore/browse/N/54+4294966708/ ) was founded by Per and Winnie Enevoldsen in Copenhagen, Denmark. Starting out as a small jewellers shop, the couple travelled to Thailand to source their jewellery, and become so popular that they switched to wholesale supply and opened their own factory in Japan to maintain control over their designs. The designers Lone Frandsen and Lisbeth Larsen were hired and Pandora began focusing on creating the unique and fun range that is now sold all over the world and is this year's must have fashion accessory. Ernest Jones, the Diamond and Watch Specialist, has over 200 stores and a retail website at www.ernestjones.co.uk. Featuring a wide range of leading brand watches and high quality jewellery (http://www.ernestjones.co.uk/webstore/jewellery.do ), Ernest Jones also has a stunning selection of gifts for all occasions. Ernest Jones is part of Signet Jewellers Limited, the world's largest speciality retail jeweller.

The Certified Professional of Occupancy (CPO) is the only comprehensive program covering the entire HUD Handbook 4350.3 including the latest revisions. Following either the NAHMA CPO Course or the AHMA East Texas Occupancy Training Program, participants must pass a rigorous one-half day exam to become certified CPOs. Those successfully passing the course receive a CPO certificate and lapel pin. The CPO is a requirement for NAHMA's NAHP certification program. Managers who have received extensive Occupancy training on HUD Handbook 4350.3, and who feel confident of their knowledge, may choose to challenge the CPO Examination. When challenging the examination, it is highly recommended that participants take the challenge exam in conjunction with a CPO Review Course. Review Courses are offered annually by AHMA East Texas and include a review of the CPO material as well as on-site proctoring for the examination. Check our Calendar page to see if a Review Course is scheduled. Challenge participants must submit an application to NAHMA to challenge the exam ($50). They must also purchase the NAHMA manual for the course to be used as a study guide. An application form to challenge the exam and purchase "Challenge" and 4350.3 manuals is provided below. Please note that the Challenge application and the manual order forms are submitted to different locations. The AHMA East Texas Occupancy Training Series is a comprehensive 4-part course providing intensive, "hands-on" training in occupancy regulations and practices to both new and experienced housing personnel - a total of 24 hours of training featuring case studies and class interaction. Students acquire practical skills that they may immediately take back to their workplace. All four courses are offered each year by AHMA East Texas. HUD Handbook 4350.3 is used as the text for these courses. Please see our Calendar for a list of courses offered this year.

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Report: Kobe Bryant will return next season Comments Off on Report: Kobe Bryant will return next season Kobe Bryant might be out for the season but he isn't ready to call it quits just yet. The Lakers confirmed on Monday that Bryant will undergo surgery to repair a torn rotator cuff in his shoulder, likely sidelining him for the remainder of the 2014-2015 campaign. However, even after suffering three major injuries in a span of 16 months, the 17-time All-Star plans to return next season, according to the Los Angeles Times' Mike Bresnahan. MORE: Kobe to have surgery | Future Laker Stanley Johnson? | Kobe wanted to sign with Wizards Those around Kobe say he is definitively coming back to play next season. Doesn't want to go out like this. — Mike Bresnahan (@Mike_Bresnahan) January 26, 2015 Bryant has fought through a number of injuries during his career and has been adament on leaving the game on his own terms. Attempting a comeback from his latest setback comes as no surprise. To sweeten the deal, Bryant is set to be the highest paid player in the NBA next season, making $25 million. In 35 games this season, Bryant averaged 22.3 points, 5.7 rebounds and 5.6 assists. At 12-and-33, the Lakers hold the fourth worst record in the NBA. Without Bryant in the fold, the Lakers' chance of landing a top pick in the draft are even greater.

Trisha Krishnan is an Indian film actress who appeared in a prominent Tamil and Telugu movies. Trisha ,her debut in Bollywood in the film opposite Akshay Kumar Khattar Meetha do. During the study, Trisha ventured into part time modeling, participating in various beauty pageants. Won Miss Salem in 1999, Miss Chennai in 1999 and Miss Brazil Miss Beautiful Smile in 2001. Trisha for the first time in 1999 in the Tamil film Jodi, as a supporting character roles, won the Filmfare Best Female Debut for her first starring role in 2002 Mouna Pesiyadhe. Later, came to fame after participating in successful films, Saamy (2003) and Ghilli (2004), in Tamil cinema and Varsham (2004) Telugu movie for which she won her first Filmfare Best Actress Award South.

Have you ever been in a room full of strangers and had the feeling they just "get you"? I am lucky enough to have experienced this in September. In September I was able to attend Cervivor School in San Diego. Cervivor School was both informative and empowering. I also, didn't think I was even going to make it. I spiked a fever the night before we were to take flight. Long story short, I talked my oncologist into sending me assuring him I could break the fever. We started out with our first night where we had a meet and greet with all our fellow Cerviors. We were serenaded by the wonderful music from two individuals who are cancer survivors who express their story through song and had time to get to know each other. It was a great laid back time to meet the other ladies. Friday kicked off with a full schedule. We did some getting to know you activities, one of which I got to co-lead with a Cervivor Ambassador Maria. It was a great time! We had each lady write down three facts about herself and each person had to guess who it was. It was a great way to get to know a large group of fabulous women. Next there was a focus on our personal stories, how to tell our stories, and how to get our stories out there in a way that can make a difference. We talked about having different versions of our story: an "elevator speech", 2 minute story, and our full story. We talked about how we might look to our audience to determine which version of our story we would be telling. We also learned about how to fully utilize social media to our advantage. It was so informative! My goal is to use more hashtags and link information together more efficiently! We also had a presentation by Dr. Ramani Durvasula, a licensed clinical psychologist in private practice in Santa Monica, CA and a Professor of Psychology at California State University, Los Angeles. Dr. Durvasula. She is featured on the video Someone You Love and she was 100% real, honest, and open. She was ready to talk to us about anything and everything. I came out of this day ready to go out and advocate for cervical cancer awareness, HPV testing, and general education. Saturday was another full schedule and I loved it! This day focused a lot around being sure we were educated with all the facts. The first person to present was a fellow cervical cancer survivor, author, and wellness expert Mary Marcdante. Our next presentation was given by Jesse Nodora, DrPH, and Ana Lilia Maldonavo, both of Project Vela and it focused on HPV, cervical cancer, health disparities and public health. It was great to have experts there, in the flesh, ready to answer our questions! Up next, Rebecca Perkins, MD, MSc Boston University School of Medicine joined us via Skype. She provided information about HPV screenings and vaccinations. She was also very informative about common misconceptions and how we can combat those misconceptions. As I assumed, there is so much misinformation out there regarding the Gardisil vaccine. Previously I would disregard people talking negatively about the vaccine so I was greatly appreciative to have so many facts provided to help me be able to combat that misinformation! Next we ended up a little bit off schedule and watched the video Someone You Love. I have previously viewed this video. I found it very difficult to watch this time. I am in a different place in my treatment right now. Knowing that my cancer has returned for the third time made it very difficult to view. There is a wonderful woman on the video who deals with a cancer reoccurrence and doesn't make it. I knew that going into the video. I left the room frequently when she was on the video. After the video we passed around the microphone and discussed our feelings after viewing the video. As you can see in the recap video the camera caught me in a rare "crying moment". As my family can attest I typically have a "no crying rule" with cancer. It was a difficult moment and I am glad I had my Cervivor family around me during this time. After the video we wrapped up our sessions for the day with "Keeping Things Sizzling" and talked about physical intamcy issues that go along with cancer. We had a fabulous dinner then we got to view a spectacular burlesque show. What a better way to finish up a long day in Cervivor School? I am so happy that I went to Cervivor School and I can't wait to expand my advocacy efforts! I should precaution you, this isn't like any post I've had before. This isn't my normal "positive cancer post". This is a raw, unfiltered, real post. This is the type of post most cancer patients keep neatly stored in his/her head and might not even bring out in a safe cancer support group. Yet, here it is. This will be hard to read if you know me personally. This might be hard to read if you don't know me personally but continue to follow my story. This post is hard to hear because it deals with mortality. It might bring up fears you have had for me that I have never really acknowledged. I don't write this lightly. I know many of my readers, I am your wife, mother (once Wylee is old enough to read this story, if he chooses), daughter, sister, granddaughter, niece, cousin, co-worker, friend. I know this is scary. I know it is something no one wants to talk about. Yet, I need to talk about it. If you've been following my cancer story you know I am generally positive about my experience. I've never really wondered why this disease chose me. I've never worried I might die, that just was never an option. I would not, could not die from this cancer. This week I found myself in the hospital, yet again. I was the sickest I had felt in the past few years. Now, part of this is my fault. Typically I go straight to the hospital as soon as I get a fever. This time I opted to live a little life and continue with my four day plans for being out of town. I made a choice and I would make the same choice again. However due to my choice, my fever and infection got worse. On my way home from California I found myself requesting a wheelchair for only the second time on this journey of cancer. I couldn't physically make it through the airport. I found myself on a plane trying to break a 103 degree fever. Finally, on the last leg of my trip I found myself physically ill and vomiting on the plane. I was so ready to get to the hospital when I finally arrived around 1:30 am on Monday morning. During that plane ride and following hospitalization I had a lot of quiet time to think. I realized I am not invincible and that this cancer could eventually kill me. It was a tough realization to come to. Now, don't take that to think I am giving up. It was a realization and feeling I needed "space to feel". I reached out to a few private support groups and they supported me through my thoughts. Today I am feeling better. I am out of the hospital. I went back to work today. I was diagnosed with another kidney infection, which is what landed me in the hospital. I have had my stent exchanged again and we have a plan moving forward. Moving forward we are going to do IV antibiotics from home for ten days and then oral antibiotics to try and keep me out of the hospital. Once we wrap up this infection we can start managing my cancer again using the Avastin. I can continue living my life with cancer. I'm thankful I took the time to see my own mortality but please know, I am not going anywhere anytime soon. This cancer is going to either stay small and unimportant or we are going to go on the aggressive offensive and attack it again. This woman isn't going down without a fight. As I stated earlier: I'm once again not worried I might die, it isn't an option. I will not, cannot die from this cancer. Thank everyone for your continued support! Please look forward to a post about the fabulous time I had at Cervivor School in San Diego!

Professor Steven Sherwood and Dr John Church will attend the Intergovernmental Panel on Climate Change (IPCC) Working Group 1 Scoping Meeting in Addis Ababa in May. Our own Dr Melissa Hart has recently been accepted into the global women in STEMM leadership initiative - Homeward Bound. CCRC staff win Excellence Award for gender equity talks. Congratulations to Dr. Angela Maharaj, A/Prof Lisa Alexander, Dr. Climate and weather warming together. Weatherzone. March 8. (Prof David Karoly, Dr Andrew King, Dr Sarah Perkins-Kirkpatrick). Extreme heatwave days already hitting poorer nations more than rich. Sydney Morning Herald. (Dr Nicholas Herold). Huge experiment: the continent that climate change has not forgotten. Sydney Morning Herald. March 4. (Prof Andy Hogg, Peof Matthew England). Sydney's swelter has a climate change link, scientists say. New York Times, Gulf News. March 2. (Dr Sarah Perkins-Kirkpatrick, Prof David Karoly). Warming had clear hand in record Australian heat. Climate Central. March 2. (Dr Sarah Perkins-Kirkpatrick, Dr Andrew King). The Guardian. March 2. (Dr Sarah Perkins-Kirkpatrick, Dr Andrew King).

Investment-Based Immigration to the U.S. The EB-5 preference category allows investors a direct path to attaining permanent residence in the United States for themselves, their spouses and their unmarried children. EB-5 – The EB-5 immigrant investor visas allow investors and their immediate family members to get permanent residence (Greencards) with a minimum $1 million investment in a business that also creates at least 10 jobs for U.S. workers. The investment can in either in a new, restructured or reorganized business, or an existing business that was expanded by forty percent. The investment can be as little as $500,000 if it is made within a "Regional Center" or "Targeted Employment Area." Utah has a number of these areas that qualify as Targeted Employment Areas. EB-5 permanent residence is initially granted as a conditional residence for two year. After the two-year period, the Investor must file an application to remove conditions and get permanent greencard. E-2 – Non-immigrant investor visas allow investors from Treaty Countries to enter the United States for a period of two years with the possibility of subsequent renewals. The level of investment can be as low as $100,000. Entry to the United States must be for the purpose of running the business. Both of these avenues for entering the United States are fairly complex, and could easily complicate your plans for developing a business and attaining permanent residence or non-immigrant status in the United States. Experienced and skilled attorneys can make sure teh process is handled correctly so that your investment, time and efforts are protected. At Preston & Brar LLC, we offer our clients skilled and experienced immigration counsel that understands all of the complexities and possible mistakes that could create unnecessary and costly challenges for our investor clients. Our firm often receives referrals from other immigration law firms and attorneys who are seeking representation they can depend on when there is no room for error. Our skill and meticulous attention to detail help our clients achieve their immigration goals. For skilled assistance in dealing with the immigration aspects of bringing your investment capital to the United States, turn to the skilled and experienced immigration lawyers of Preston & Brar LLC. To schedule a free initial consultation, call us at 801-269-9541 or 801-577-1988 (cell) or contact us online.

UTILS_HOME = ../../utils CLOSURE ?= $(UTILS_HOME)/closure.jar BUILD_DIR = ../../build/firefox RESTARTLESS_DIR = ../../build/firefox-restartless PROFILE ?= $(error Specify Firefox profile in PROFILE) BUNDLES_DIR = ../../build/bundles-cc # Just builds a ff extension VERSION = `head -n 1 ../../build/version/version.txt` build: ensureoutputdir # Copying extension files echo "Creating extension version: $(VERSION)" cp -R ../../LICENSE ../../LICENSE-OFL.txt ../../README-LICENSE.txt content chrome bootstrap.js chrome.manifest icon.png icon64.png $(BUILD_DIR)/ sed s/\(SHUMWAY_VERSION\)/$(VERSION)/ install.rdf > $(BUILD_DIR)/install.rdf sed s/\(SHUMWAY_VERSION\)/$(VERSION)/ update.rdf > $(BUILD_DIR)/update.rdf cp ../../examples/inspector/debug/pingpong.js $(BUILD_DIR)/chrome/ # Copying JavaScript content files mkdir -p $(BUILD_DIR)/content/playerglobal cp ../../build/playerglobal/playerglobal.json $(BUILD_DIR)/content/playerglobal/ cp ../../build/playerglobal/playerglobal.abcs $(BUILD_DIR)/content/playerglobal/ # Copying ABC and Relooper files mkdir -p $(BUILD_DIR)/content/libs cp ../../build/libs/builtin.abc $(BUILD_DIR)/content/libs/ cp ../../build/libs/relooper.js $(BUILD_DIR)/content/libs/ # Copying closure optimized shumway.js files cp $(BUNDLES_DIR)/shumway*.js $(BUILD_DIR)/content/ cp ../../build/version/version.txt $(BUILD_DIR)/content/version.txt echo "extension" >> $(BUILD_DIR)/content/version.txt # Packaging XPI file cd $(BUILD_DIR); zip -r shumway.xpi * ensureoutputdir: clean -mkdir -p $(BUILD_DIR) clean: -rm -rf build -rm -rf $(BUILD_DIR) -rm -rf $(RESTARTLESS_DIR) restartless: -rm -rf $(RESTARTLESS_DIR) mkdir -p $(RESTARTLESS_DIR) cd $(RESTARTLESS_DIR); find ../../extension/firefox -type f -maxdepth 1 -mindepth 1 -exec ln -s {} \; mkdir -p $(RESTARTLESS_DIR)/chrome cd $(RESTARTLESS_DIR)/chrome; find ../../../extension/firefox/chrome -maxdepth 1 -mindepth 1 -exec ln -s {} \; mkdir -p $(RESTARTLESS_DIR)/content cd $(RESTARTLESS_DIR)/content; find ../../../extension/firefox/content -maxdepth 1 -mindepth 1 -exec ln -s {} \; ln -s ../../../examples/inspector/debug/pingpong.js $(RESTARTLESS_DIR)/chrome/pingpong.js ln -s ../../../src/gfx $(RESTARTLESS_DIR)/content/gfx ln -s ../../../build/playerglobal $(RESTARTLESS_DIR)/content/playerglobal ln -s ../../../build/libs $(RESTARTLESS_DIR)/content/libs ln -s ../../../build/bundles/shumway.gfx.js $(RESTARTLESS_DIR)/content/shumway.gfx.js ln -s ../../../build/bundles/shumway.player.js $(RESTARTLESS_DIR)/content/shumway.player.js ln -s ../../../build/version/version.txt $(RESTARTLESS_DIR)/content/version.txt cd $(RESTARTLESS_DIR); pwd > "$(PROFILE)/extensions/shumway-dev@research.mozilla.org" grep "nglayout.debug.disable_xul_cache\", true" "$(PROFILE)/prefs.js" 1>/dev/null || echo "Set create 'nglayout.debug.disable_xul_cache' boolean preference to 'true'." PHONY: build clean ensureoutputdir restartless

Want to prove to your friend that the lyrics are "Big ol' jet airliner" and not "Bingo jet had a light on"? My brother years ago told me about his friend who swore that the lyrics to Jet Airliner included "Bingo Jet had a light on". Well, that was years ago and now Apple has added to their music app the ability to see the lyrics to a song you are listening to. Simply select the 3 dots on the bottom right of the screen and select lyrics. Below is a a very quick GIF of my kids favorite song now, Hall of Fame by The Script, followed by an 18 second video that I created using QuickTime Player. Any questions, let me know. Enjoy proving you are right - or now finally figuring out what that artist is really saying. Don't forget that you can also use Siri to listen to a song and tell you who is singing it. Click here for a post I created about it back in August.

Our goal is to secure your investments and ensure their lasting success. This is why we have a comprehensive package of after-sales services for you. Our range of maintenance and after-sales services covers all components and all levels of an automation solution, from field devices, switchgear, PLC and process control technology through to operational data management systems. Whether preventive maintenance or reactive repairs, even with 24x7 on-call standby, guaranteed response time and stocking of spare parts – our after-sales service team will be happy to meet your needs. When it comes to service, people make the difference. On completion of each project, we therefore appoint a person who is responsible for the respective plant and is the permanent and fixed contact person for our customer.

Beck's Basic Moves By Brad Gruetzmacher - Mighty No. 9 has had a busy couple of weeks. After the announcement of a new animated series in the works, the team at comcept have decided to get back to the basics and reveal more about the main character Beck. In our last update we were treated to some information about Beck's sidekick Call and her character animations. Now we learn more information about the main hero Beck and some of his basic moves. The first video being shared shows off Beck's basic actions of running/jumping/shooting. As fans of platformers know, each game takes a different approach to jump mechanics and running. LittleBigPlanet's floaty jumps in despised by some and loved by others, while Mario has a much tighter control. It would appear that Beck runs with a much looser style, but you can judge for yourself in this newly released video. In the second video we are treated to seeing Beck and his climbing abilities. This includes both going up and down ladders, but also dangling from ledges. It gives a good idea as to how Beck will shoot while traversing both up and down. Video three shows off Beck's dash attack, which is an ability that we've learned Call will not have. The dash will not only be useful for attacks, but also to gain access to tight spaces. And finally we get a look at Beck's "absorb" ability. Once Beck has worn down an enemy sufficiently enough, he can dash into them in order to absorb their "Xel" which can then be converted into power-ups. This is an ability that is unique to Beck "and it's this ability he has to use in order to save everybody." As each video warns though, this is a work in progress so the final game may not turn out exactly as these videos portray. More information on Mighy No. 9 can be expected in future updates from comcept, and the game is due for release in April 2015. VIAGematsu SOURCEKickstarter COMCEPT MIGHTY NO 9 Previous article"Volume" from Mike Bithell Delayed into 2015 Next article99 Cent Flash Sale On PSN Brad Gruetzmacher http://thevitalounge.net Brad is a video game enthusiast and family man. He's been gaming since the days of the Intellivision, and while that indicates he's been doing this for quite some time, he doesn't intend to quit anytime soon. Currently he's trying desperately to convince his daughter that there are more games than just Minecraft (unsuccessfully so far). nonscpo I found these trailers to be average, nothing that much special from what we've seen already. Kinda wish they showed more background music. I wonder what game play hook Call is going to have. I hope it's more fun than instant hard mode. vongruetz . So far the only one I know of is her ability to crawl, which takes the place of Beck's dash attack. But since the game is still a year away, I'm sure more surprises are in store. I sure hope so! So it is a straight Mega Man clone. Croixleur Sigma PlayStation TV Review Bloxiq

The Blom Tracheostomy Tube System features a variety of unique inner cannulas that aid in the clearance and management of secretions, to help prevent Ventilator associated events. These unique inner cannulas also help to provide Speech to ventilator dependent patients as well as patients off of the vent. Smooth fenestration with no sharp edges located just 1mm from the cuff to prevent fenestration contact with the tracheal mucosa. Allows for assessment of the patient's ability to breathe through the normal oral/nasal route, preparing the patient for deccanulation and maximizing air exchange during the weaning process. Provides airway protection and bronchial hygiene, as well as facilitates speech. The inner cannula has a suction catheter attached to the outside. This allows it to removes secretions (accumulated above the cuff) through the fenestration of the Blom Fenestrated Cuffed Tracheostomy Tube. It can be used at a continuous suction of 20-30mmHg or an intermittent cycled suction at 100-150mmHg. The suction line cap allows for intermittent manual suctioning when needed. The LPV is a hygienic alternative to finger occlusion and helps patients that are off of the ventilator to speak. It uses a flap valve which opens during inhalation and remains closed during exhalation redirecting air to the vocal cords. The hub of the LPV sits right up next to the Tracheostomy Tube creating a "Low Profile" appearance. It will not protrude from the neck. The LPV can be used with any Blom Tracheostomy Tube.

Q: Problems with rsGetElementAt_uchar4 I'm trying to implement a median filter in RenderScript. But the work of the code is not stable. Reading elements not from the current line rsGetElementAt_uchar4 (inputAlloc, x , y + a ) causes errors. What is the problem ? Is there an example of such a filter using RenderScript? #pragma version(1) #pragma rs java_package_name(a.myapplication) #pragma rs_fp_relaxed rs_allocation inputAlloc; int bWidht, bHeight; static uchar4 arrpix[9]; static uchar4 buff; uchar4 __attribute__((kernel)) median(uchar4 in, uint32_t x, uint32_t y) { uchar4 arrpix[9]; uchar4 buff; if((x<bWidht) && (y<bHeight)){ arrpix[0] = rsGetElementAt_uchar4(inputAlloc, x -1 , y - 1); arrpix[1] = rsGetElementAt_uchar4(inputAlloc, x , y - 1); arrpix[2] = rsGetElementAt_uchar4(inputAlloc, x +1 , y - 1); arrpix[3] = rsGetElementAt_uchar4(inputAlloc, x -1 , y ); arrpix[4] = in; arrpix[5] = rsGetElementAt_uchar4(inputAlloc, x +1 , y ); arrpix[6] = rsGetElementAt_uchar4(inputAlloc, x -1 , y + 1); arrpix[7] = rsGetElementAt_uchar4(inputAlloc, x , y + 1); arrpix[8] = rsGetElementAt_uchar4(inputAlloc, x +1 , y + 1); for(int i=0; i<4; i++) for(int i=0; i<=8; i++){ if(arrpix[i].r>arrpix[i+1].r){ buff.r = arrpix[i].r; arrpix[i].r = arrpix[i+1].r; arrpix[i+1].r = buff.r;} if(arrpix[i].g>arrpix[i+1].g){ buff.g = arrpix[i].g; arrpix[i].g = arrpix[i+1].g; arrpix[i+1].g = buff.g;} if(arrpix[i].b>arrpix[i+1].b){ buff.b = arrpix[i].b; arrpix[i].b = arrpix[i+1].b; arrpix[i+1].b = buff.b;} } } return arrpix[4]; } A: You need to check x>0 and y>0 because 0-1 =-1 Bottom loops don't look completely correct either. Can you fix the spacing and did you mean to use i in both loops?

Background Born in Puerto Rico. Trained at Johnson & Wales University in Norfolk, Virginia. Worked at The Frog and the Redneck in Richmond, Virginia, and The Ritz-Carlton, Buckhead, in Atlanta. How he got into cooking "There weren't always waves to surf in Virginia Beach, where I grew up, so I needed something else to do." First food memory Barbecued wild pig, grilled catfish and venison sausage at his uncle's ranch in Texas, age eight. Mentors Chefs Jimmy Sneed and Guenther Seeger. Menu bomb "I made an awesome rabbit terrine. I was loving it, but I couldn't give it away." What he'd be if he weren't a chef An oceanographer. Favorite cookbook Jacques Pépin's La Technique. Favorite place to eat Soto in Atlanta. Dish he could eat every day Mashed potatoes. Trend he's most tired of "People trying to do the Asian thing—it's like a contagious disease. There should be a law." Favorite kitchen tool A Vita-Mix blender."The best. It will puree rocks." Food vices Barbecue ("In Richmond, that's a vice") and Burger King. Recipe tip When sautéeing onions or shallots, add a pinch of salt first; it helps release their juices and prevents burning.

Hope earned her Ph.D., M.A. and M.B.A. from the University of California, Irvine, and her B.S. from California State Long Beach. She has published well-cited research on the impact of technology on marketplace relationships, branding, identity-salient consumption practices, and collaborative value creation. An award-winning instructor, she teaches marketing management, managing marketing communications, and social media marketing strategy in the Eller M.B.A. programs. She is a popular visiting scholar at institutions across the globe, including the University of Melbourne (Australia), the University of Hawaii, the University of Auckland (New Zealand), and Fundaçäo Getúlio Vargas (Brazil). Her work has appeared in the Journal of Marketing, Journal of Consumer Research, Journal of Retailing, Journal of Advertising, and the Journal of Public Policy and Marketing, among others. She serves on the editorial review boards of the Journal of Consumer Research and Consumption Markets and Culture. Hope Jensen Schau Associate Dean, M.B.A. Programs, an associate professor of marketing, and holds the Gary M. Munsinger Chair in Entrepreneurship and Innovation at the Eller College of Management, University of Arizona Epp, Amber, Hope Jensen Schau and Linda L. Price (2014), "The Role of Brands and Mediating Technologies in Assembling Long-Distance Family Practices," Journal of Marketing, 78: 81-101 Muñiz, Albert M. Jr. and Hope Jensen Schau (2011), "How to Inspire Value-Laden Collaborative Consumer-Generated Content," Business Horizons, 54 (3), 209-217. Muñiz, Albert M. Jr. and Hope Jensen Schau (2007), "Vigilante Marketing and Consumer-created Communications," Journal of Advertising, 36(3): 187-202. Muñiz, Albert M. Jr. and Hope Jensen Schau (2005), "Religiosity in the Abandoned Apple Newton Brand Community," Journal of Consumer Research, 31(4): 737-747. Russell, Cristel Antonia and Hope Jensen Schau (2014), "When Narrative Brands End: The Impact of Narrative Closure and Consumption Sociality on Loss Accommodation," the Journal of Consumer Research, 40: 1039-1062 Schau, Hope Jensen, Albert M. Muñiz Jr. and Eric J. Arnould (2009), "How Brand Community Practices Create Value," Journal of Marketing, 73(5): 30-51 Schau, Hope Jensen and Mary C. Gilly (2003), "We Are What We Post? The Presentation of Self in Personal Webspace," Journal of Consumer Research, 30(4): 385-404. Thomas, Tandy Chalmers, Linda L. Price and Hope Jensen Schau (2013), "When Differences Unite: Resource Dependence in Heterogeneous Consumption Communities," Journal of Consumer Research 39(5): 1010-1033 Fellow Eric Arnould Fellow Matt Thomson

The Pastel Piebald is a co-dom recessive morph combination, we produced it in 2005 along with The Snake Keeper. After missing the odds on multiple clutches, our luck changed with the second to last clutch of the season, from a 5 egg clutch of Pastel het Pied x het Pied, out came one of our prized possessions one of the first Pastel Pieds. You can imagine the excitement and joy that was felt on that Labor Day holiday in 2005, when we discovered this beautiful Pastel Pied had hatched and it was a male. This male has grown up and in 2008 had sired the first Super Pastel Pied or "Killer Pied", a stunning lemon yellow Piebald, creating a greater demand for the already sought after Pastel Pied.