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26903598

Comparison of patients with early-phase arrhythmogenic right ventricular cardiomyopathy and right ventricular outflow tract ventricular tachycardia.

AIMS Differentiation between early-phase arrhythmogenic right ventricular cardiomyopathy (ARVC) and right ventricular outflow tract (RVOT)-ventricular tachycardia (VT) can be challenging, and correct diagnosis is important. We compared electrocardiogram (ECG) parameters and morphological right ventricular (RV) abnormalities and investigated if ECG and cardiac imaging can help to discriminate early-phase ARVC from RVOT-VT patients. METHODS AND RESULTS We included 44 consecutive RVOT-VT (47 ± 14 years) and 121 ARVC patients (42 ± 17 years). Of the ARVC patients, 77 had definite ARVC and 44 had early-phase ARVC disease. All underwent clinical examination, ECG, and Holter monitoring. Frequency of premature ventricular complexes (PVC) was expressed as percent per total beats/24 h (%PVC), and PVC configuration was recorded. By echocardiography, we assessed indexed RV basal diameter (RVD), indexed RVOT diameter, and RV and left ventricular (LV) function. RV mechanical dispersion (RVMD), reflecting RV contraction heterogeneity, was assessed by speckle-tracking strain echocardiography. RV ejection fraction (RVEF) was assessed by cardiac magnetic resonance imaging (CMR). Patients with early-phase ARVC had lower %PVC by Holter and PVC more frequently originated from the RV lateral free wall (both P < 0.001). RVD was larger (21 ± 3 vs. 19 ± 2 mm, P < 0.01), RVMD was more pronounced (22 ± 15 vs. 15 ± 13 ms, P = 0.03), and RVEF by CMR was decreased (41 ± 8 vs. 49 ± 4%, P < 0.001) in early-phase ARVC vs. RVOT-VT patients. CONCLUSION Patients with early-phase ARVC had structural abnormalities with lower RVEF, increased RVD, and pronounced RVMD in addition to lower %PVC by Holter compared with RVOT-VT patients. These parameters can help correct diagnosis in patients with unclear phenotypes.

['Saberniak|Jørg|J|', 'Leren|Ida S|IS|', 'Haland|Trine F|TF|', 'Beitnes|Jan Otto|JO|', 'Hopp|Einar|E|', 'Borgquist|Rasmus|R|', 'Edvardsen|Thor|T|', 'Haugaa|Kristina H|KH|']

2017

32061506

Risk assessment in pulmonary arterial hypertension: Insights from the GRIPHON study.

BACKGROUND Approaches to risk assessment in pulmonary arterial hypertension (PAH) include the noninvasive French risk assessment approach (number of low-risk criteria based on the European Society of Cardiology and European Respiratory Society guidelines) and Registry to Evaluate Early and Long-term PAH Disease Management (REVEAL) 2.0 risk calculator. The prognostic and predictive value of these methods for morbidity/mortality was evaluated in the predominantly prevalent population of GRIPHON, the largest randomized controlled trial in PAH. METHODS GRIPHON randomized 1,156 patients with PAH to selexipag or placebo. Post-hoc analyses were performed on the primary composite end-point of morbidity/mortality by the number of low-risk criteria (World Health Organization functional class I-II; 6-minute walk distance >440 m; N-terminal pro-brain natriuretic peptide <300 ng/liter) and REVEAL 2.0 risk category. Hazard ratios and 95% confidence intervals were calculated using Cox proportional hazard models. RESULTS Both the number of low-risk criteria and the REVEAL 2.0 risk category were prognostic for morbidity/mortality at baseline and any time-point during the study. Patients with 3 low-risk criteria at baseline had a 94% reduced risk of morbidity/mortality compared to patients with 0 low-risk criteria and were all categorized as low-risk by REVEAL 2.0. The treatment effect of selexipag on morbidity/mortality was consistent irrespective of the number of low-risk criteria or the REVEAL 2.0 risk category at any time-point during the study. Selexipag-treated patients were more likely to increase their number of low-risk criteria from baseline to week 26 than placebo-treated patients (odds ratio 1.69, p = 0.0002); similar results were observed for REVEAL 2.0 risk score. CONCLUSIONS These results support the association between risk profile and long-term outcome and suggest that selexipag treatment may improve risk profile.

['Sitbon|Olivier|O|', 'Chin|Kelly M|KM|', 'Channick|Richard N|RN|', 'Benza|Raymond L|RL|', 'Di Scala|Lilla|L|', 'Gaine|Sean|S|', 'Ghofrani|Hossein-Ardeschir|HA|', 'Lang|Irene M|IM|', 'McLaughlin|Vallerie V|VV|', 'Preiss|Ralph|R|', 'Rubin|Lewis J|LJ|', 'Simonneau|Gérald|G|', 'Tapson|Victor F|VF|', 'Galiè|Nazzareno|N|', 'Hoeper|Marius M|MM|']

2020

33910477

Word imageability and orthographic neighbourhood effects on memory: a study in free recall and recognition.

This study investigated the effects of word imageability and orthographic neighbourhood size, as well as their combined effects, in free recall and recognition memory. A total of 45 young adults performed recall and recognition tasks on the same word materials. Word imageability and orthographic neighbourhood size were orthogonally manipulated across four word conditions: low-imageability words - high N, saveur [flavor], low-imageability words - low N, total [total], high-imageability words - high N, carré [square] and high-imageability - low N, nuage [cloud]. The results show that word imageability facilitates memory performance in both free recall and recognition tasks, while the effect of orthographic neighbourhood size was exhibited only in the recognition task. Finally, the orthographic neighbourhood effect was found to depend on word imageability. The implications of the results are discussed according to semantic and orthographic word characteristics with regard to the memory processes involved in free recall and recognition tasks.

['Ballot|Claire|C|', 'Mathey|Stéphanie|S|', 'Robert|Christelle|C|']

2021

28401587

Valuation of Life as outcome and mediator of a depression intervention for older African Americans: the Get Busy Get Better Trial.

OBJECTIVE Previously, we showed that Get Busy Get Better (GBGB), a 10-session multicomponent home-based, behavioral intervention, reduced depressive symptom severity in older African Americans. As appraising the value of life is associated with depressive symptoms, this study examined whether GBGB enhanced positive appraisals of life and if, in turn, this mediated treatment effects on depressive symptoms. METHODS Data were from a single-blind parallel randomized trial involving 208 African Americans (≥55 years old) with depressive symptoms (Patient Health Questionnaire, PHQ-9 ≥5). GBGB involved five components: care management, referral/linkage, stress reduction, depression education, and behavioral activation. A 13-item Valuation of Life (VOL) scale with two subfactors (optimism and engagement) was examined as an outcome and as mediating GBGB effects on PHQ-9 scores at 4 months. RESULTS Of 208 enrolled African Americans, 180 completed the 4-month interview (87 = GBGB; 93 = control). At 4 months, compared with wait-list control group participants, the GBGB group had improved VOL (difference in mean changes from baseline = 4.67, 95% confidence interval 2.53, 6.80). Structural equation models indicated that enhanced VOL mediated a significant proportion of GBGB's impact on depressive symptoms, explaining 71% of its total effect, and its subfactors (optimism, explaining 67%; engagement, 52%). CONCLUSION Valuation of Life appears malleable through an intervention providing resources and activation skills. GBGB's impact on depressive symptoms is attributed in large part to participants' enhanced attachment to life. Attention to VOL as mediator and outcome and the reciprocal relationship between mood and attachment to life is warranted. Copyright © 2017 John Wiley & Sons, Ltd.

['Gitlin|Laura N|LN|http://orcid.org/0000-0003-2869-473X', 'Parisi|Jeanine M|JM|', 'Huang|Jin|J|', 'Winter|Laraine|L|', 'Roth|David L|DL|']

2018

28571758

Killer-cell immunoglobulin-like receptor (KIR) and human leukocyte antigen (HLA) class I genetic diversity in four South African populations.

Killer-cell Immunoglobulin-like Receptor (KIR) and Human Leukocyte Antigen (HLA) genotypes vary considerably between individuals and populations due to KIR/HLA allelic variation and variable haplotype configurations of KIR. HLA mediate natural killer cell activity by serving as KIR ligands. KIR/HLA polymorphisms associate with both disease susceptibility and severity. We determined the frequencies of KIR, KIR genotypes and KIR-HLA combinations in 364 healthy individuals from four South African populations. Study participants included black African (n=167), Caucasian (n=97), Mixed ancestry (n=50) and Indian (n=50) individuals. We identified 48 KIR genotypes that included two genotypes not previously reported. Based on KIR gene content, Indian individuals represented the most distinct group, showing the highest frequencies of KIR2DL2, KIR2DL5, KIR2DS1, KIR2DS2, KIR2DS3 and KIR3DS1, the lowest frequencies of KIR2DL3, KIR2DS4 and KIR3DL1; and a KIR2DL4-negative individual. KIR2DS1 and KIR3DS1 were infrequent in black African populations. HLA-C2 was more common in black African individuals, while HLA-C1 predominated in the other populations. Indian individuals were more likely to possess KIR2DL2 paired with HLA-C1, while Caucasian individuals exhibited the highest frequencies of KIR2DL3 paired with HLA-C1. This report provides comprehensive reference data for further study of the roles of KIR/HLA in non-communicable and infectious diseases in South African populations.

['Gentle|Nikki L|NL|', 'Loubser|Shayne|S|', 'Paximadis|Maria|M|', 'Puren|Adrian|A|', 'Tiemessen|Caroline T|CT|']

2017

28052347

Apoptotic effects of bovine apo-lactoferrin on HeLa tumor cells.

Lactoferrin (Lf), a cationic iron-binding glycoprotein of 80 kDa present in body secretions, is known as a compound with marked antimicrobial activity. In the present study, the apoptotic effect of iron-free bovine lactoferrin (apo-bLf) on human epithelial cancer (HeLa) cells was examined in association with reactive oxygen species and glutathione (GSH) levels. Apoptotic effect of iron-free bovine lactoferrin inhibited the growth of HeLa cells after 48 hours of treatment while the diferric-bLf was ineffective in the concentration range tested (from 1 to 12.5 μM). Western blot analysis showed that key apoptotic regulators including Bax, Bcl-2, Sirt1, Mcl-1, and PARP-1 were modulated by 1.25 μM of apo-bLf. In the same cell line, apo-bLf induced apoptosis together with poly (ADP-ribose) polymerase cleavage, caspase activation, and a significant drop of NAD+ . In addition, apo-bLf-treated HeLa cells showed a marked increase of reactive oxygen species level and a significant GSH depletion. On the whole, apo-bLf triggered apoptosis of HeLa cells upon oxygen radicals burst and GSH decrease.

['Luzi|Carla|C|', 'Brisdelli|Fabrizia|F|', 'Iorio|Roberto|R|', 'Bozzi|Argante|A|', 'Carnicelli|Veronica|V|', 'Di Giulio|Antonio|A|', 'Lizzi|Anna Rita|AR|']

2017

31519619

Keratinocyte Carcinoma and Risk for Another Type of Cancer: Assessment of a Dose-response Relationship.

BACKGROUND/AIM Keratinocyte carcinoma (KC) is a marker of increased risk of other cancer types. To assess if this association exhibits a dose-response relationship, a case-control study was carried out. PATIENTS AND METHODS This was a clinic-based study of cases with KC plus another type of cancer matched by age, race (all Caucasian), sex and histologic type to controls with KC only (n=48 matched pairs). RESULTS Compared with the KC only group, those with KC plus another cancer had a mean number of lesions that were 43%, 35%, and 41% greater for basal cell carcinoma (BCC), squamous cell carcinoma (SCC), and total KC, respectively. The odds ratio (OR) of developing another type of cancer increased from 1.0 to 1.09 (95% confidence interval (CI)=0.23-5.13) to 2.12 (95%CI=0.50-9.08) according to whether the patient had zero, one, or ≥two BCC lesions; for SCC, the corresponding ORs were 1.0, 1.24 (95%CI=0.48-3.24), and 1.39 (95%CI=0.29-6.61). CONCLUSION A dose-response relationship seems to exist between the number of skin lesions and the risk of another type of cancer, but the lack of statistical significance weakens this evidence.

['Wilson|Ashley|A|', 'Small|James|J|', 'Flanagan|Catherine|C|', 'Perry|David|D|', 'Marchell|Richard|R|', 'Thiers|Bruce|B|', 'Alberg|Anthony J|AJ|']

2019

27885451

Increased nucleophosmin expression is a strong predictor of recurrence and prognosis in patients with N0M0 upper tract urothelial carcinoma undergoing radical nephroureterectomy.

PURPOSE We aimed to evaluate whether increased nucleophosmin expression predicts recurrence and survival in upper tract urothelial carcinoma (UTUC). METHODS Specimens from 101 patients with N0M0 UTUC undergoing radical nephroureterectomy were evaluated. Nucleophosmin expression was determined immunohistochemically and categorized into two groups according to nucleophosmin staining intensity. The association between nucleophosmin expression and various clinicopathological factors including Ki-67 expression was analyzed. Multivariate analyses were performed to identify the independent predictors of extraurothelial recurrence and cancer-specific survival. RESULTS High nucleophosmin expression was significantly correlated with tumor location, pT ≥3, lymphovascular invasion, lymph node metastasis, and high Ki-67 expression. Patients whose tumors demonstrated high nucleophosmin expression had a significantly higher rate of extraurothelial recurrence and a lower survival rate than those with low nucleophosmin expression. Multivariate analysis showed that pT ≥3, lymph node metastasis, high nucleophosmin expression, and high Ki-67 expression were independent predictors of extraurothelial recurrence. When patients were stratified into three groups according to the number of risk factors, the 2-year extraurothelial recurrence-free survival rates were 92.9% in patients with 0 or 1 risk factor, 76.5% in patients with 2 risk factors, and 9.1% in patients with 3 or 4 risk factors. Regarding cancer-specific survival, lymphovascular invasion and high nucleophosmin expression were independent predictors. CONCLUSIONS Increased nucleophosmin expression was a strong predictor of extraurothelial recurrence and cancer-specific survival in patients with N0M0 UTUC undergoing radical nephroureterectomy. Our risk stratification models integrating nucleophosmin expression may provide valuable information on disease recurrence and prognosis.

['Sawazaki|Harutake|H|', 'Ito|Keiichi|K|', 'Asano|Takako|T|', 'Kuroda|Kenji|K|', 'Sato|Akinori|A|', 'Asakuma|Junichi|J|', 'Horiguchi|Akio|A|', 'Seguchi|Kenji|K|', 'Asano|Tomohiko|T|']

2017

32216060

Omental apoplexy: Unravelling the mystery.

INTRODUCTION To describe cases omental haemorrhage and to review the literature on this topic. METHODS We describe three cases of spontaneous omental haemorrhage and discuss various management strategies, in an attempt to provide direction for similar cases in the future. RESULTS A number of case reports of spontaneous or idiopathic omental haemorrhage exist in the literature. These cases are often attributed to an underlying vasculopathy, such as segmental arterial mediolysis (SAM). Appropriate resuscitation is paramount for best outcome. Severe bleeding may require surgery or transcatheter arterial embolisation, which is best performed early if required. Endovascular management using selective catheterisation of the bleeding vessel and embolisation is a minimally invasive alternative to emergent operative intervention. In the three cases we present, endovascular embolisation was performed in two patients, and surgical ligation in a third. Segmental arterial mediolysis is considered the likely aetiology in at least 2 of the 3 cases, based on imaging findings. No further episodes of haemorrhage occurred at follow-up (ranging from 6 months to 2 years). CONCLUSIONS Acute omental haemorrhage is a rare condition; however, it may be associated with significant morbidity and mortality. CT angiography is the imaging of choice. Management strategies include both endovascular and surgical intervention.

['Moriarty|Heather K|HK|https://orcid.org/0000-0001-7441-0334', 'Martin|Katherine|K|', 'Koukounaras|Jim|J|https://orcid.org/0000-0001-9795-9477', 'Goh|Gerard S|GS|https://orcid.org/0000-0002-1552-5470', 'Clements|Warren|W|https://orcid.org/0000-0003-1859-5850']

2020

29413513

Risk factors for nosocomial infection among hospitalised severe influenza A(H1N1)pdm09 patients.

BACKGROUND AND OBJECTIVE Nosocomial infections following influenza are important causes of death, requiring early implementation of preventive measures, but predictors for nosocomial infection in the early stage remained undetermined. We aimed to determine risk factors that can help clinicians identify patients with high risk of nosocomial infection following influenza on admission. METHOD Using a database prospectively collected through a Chinese national network for hospitalised severe influenza A(H1N1)pdm09 patients, we compared the characteristics on admission between patients with and without nosocomial infection. RESULT A total of 2146 patients were enrolled in the final analysis with a median age of 36.0 years, male patients comprising 50.2% of the sample and 232 (10.8%) patients complicated with nosocomial infection. Acinetobacter baumannii, Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Staphylococcus aureus were the leading pathogens, and invasive fungal infection was found in 30 cases (12.9%). The in-hospital mortality was much higher in patients with nosocomial infection than those without (45.7% vs 11.8%, P < 0.001). Need for mechanical ventilation (OR: 3.336; 95% CI 2.362-4.712), sepsis (OR: 2.125; 95% CI 1.236-3.651), ICU admission on first day (OR: 2.074; 95% CI 1.425-3.019), lymphocytopenia (OR: 1.906; 95% CI 1.361-2.671), age > 65 years (OR: 1.83; 95% CI 1.04-3.21) and anaemia (OR: 1.39; 95% CI 1.39-2.79) were independently associated with nosocomial infection. CONCLUSION Need for mechanical ventilation, sepsis, ICU admission on first day, lymphocytopenia, older age and anaemia were independent risk factors that can help clinicians identify severe influenza A(H1N1)pdm09 patients at high risk of nosocomial infection.

['Zhou|Fei|F|', 'Li|Hui|H|', 'Gu|Li|L|', 'Liu|Meng|M|', 'Xue|Chun-Xue|CX|', 'Cao|Bin|B|', 'Wang|Chen|C|', '|||']

2018

29873527

Magnetic Fe3O4@MCM-41 core-shell nanoparticles functionalized with thiol silane for efficient l-asparaginase immobilization.

l-Asparaginase (l-ASNase) is a vital enzyme for medical treatment and food industry. Here, we assessed the use of Fe3O4@Mobil Composition of Matter No. 41 (MCM-41) magnetic nanoparticles as carrier matrix for l-ASNase immobilization. In addition, surface of Fe3O4@MCM-41 magnetic nanoparticles was functionalized with 3-mercaptopropyltrimethoxysilane (MPTMS) to enhance stability of l-ASNase. The chemical structure, thermal properties, magnetic profile and morphology of the thiol-functionalized Fe3O4@MCM-41 magnetic nanoparticles were characterized with Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential thermal analysis (DTA), differential scanning calorimetry (DSC), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), energy dispersive X-ray (EDX) spectroscopy and zeta-potential measurement. l-ASNase was covalently immobilized onto the thiol-functionalized Fe3O4@MCM-41 magnetic nanoparticles. The properties of the immobilized enzyme, including optimum pH, temperature, kinetic parameters, thermal stability, reusability and storage stability were investigated and compared to free one. Immobilized enzyme was found to be stable over a wide range of pH and temperature range than free enzyme. The immobilized l-ASNase also showed higher thermal stability after 180 min incubation at 50 °C. The immobilized enzyme still retained 63% of its original activity after 16 times of reuse. The Km value for the immobilized enzyme was 1.15-fold lower than the free enzyme, which indicates increased affinity for the substrate. Additionally, the immobilized enzyme was active over 65% and 53% after 30 days of storage at 4 °C and room temperature (∼25 °C), respectively. Thereby, the results confirmed that thiol-functionalized Fe3O4@MCM-41 magnetic nanoparticles had high efficiency for l-ASNase immobilization and improved stability of L-ASNase.

['Ulu|Ahmet|A|', 'Noma|Samir Abbas Ali|SAA|', 'Koytepe|Suleyman|S|', 'Ates|Burhan|B|http://orcid.org/0000-0001-6080-229X']

2018

33706096

Circular economy fertilization: Phycoremediated algal biomass as biofertilizers for sustainable crop production.

There is an urgent need to meet the demand of water and nutrients by their reuse and recycling to gratify sustainable food production system and resource conservation. Chlorella minutissima was found to be very effective in the removal of electrical conductivity (EC), total dissolved solids, phosphorous (P), potassium (K), ammonium, nitrate, biological oxygen demand (BOD5) and chemical oxygen demand (COD) of sewage wastewater. We tested the effects of phycoremediated algal biomass addition to soil in field plots of baby corn and spinach, on plant growth, yield and soil chemical properties. The application of 100% nitrogen (N) fertilizer by algal biomass lead to higher economic yield of spinach and baby corn than recommended dose of mineral fertilizers. The available N and P content in experimental plots applied with algae biomass as biofertilizers were significantly higher than other treatments. The soil enzymes, such as urease, nitrate reductase, and dehydrogenase were analysed during the cropping season of baby corn and spinach. The soil supplied with 100% N by algae biomass (C. minutissima) significantly (P < 0.05) increased the dehydrogenase activity in spinach grown soil. While the nitrate reductase activity in soil supplied with algal manure was maximum (0.13 mg NO2-N produced g-1 soil 24 h-1) and significantly higher than other treatments in baby corn grown soil. This study revealed that phycoremediation coupled with biofertilizers production from algae biomass is a recycling and resource conservation exercise to reduce eutrophication, recycling of wastewater, recycling of plant nutrients and improvement of the soil quality in circular economy fertilization.

['Sharma|Gulshan Kumar|GK|', 'Khan|Shakeel Ahmad|SA|', 'Shrivastava|Manoj|M|', 'Bhattacharyya|Ranjan|R|', 'Sharma|Anil|A|', 'Gupta|Dipak Kumar|DK|', 'Kishore|Prabhat|P|', 'Gupta|Navindu|N|']

2021

26278494

Age-related changes in the endocannabinoid system in the mouse hippocampus.

Previous studies have demonstrated that the endocannabinoid system significantly influences the progression of brain ageing, and the hippocampus is one of the brain regions most vulnerable to ageing and neurodegeneration. We have further examined age-related changes in the hippocampal endocannabinoid system by measuring the levels of anandamide (AEA) and 2-arachidonoylglycerol (2-AG) in young and old mice from two different mouse strains. We found a decrease in 2-AG but not AEA levels in aged mice. In order to identify the cause for 2-AG level changes, we investigated the levels of several enzymes that contribute to synthesis and degradation of 2-AG in the hippocampus. We found a selective decrease in DAGLα mRNA and protein levels as well as an elevated MAGL activity during ageing. We hypothesize that the observed decrease of 2-AG levels is probably caused by changes in DAGLα expression and MAGL activity. This finding can contribute to the existing knowledge about the processes underlying selective vulnerability of the hippocampus to ageing and age-related neurodegeneration.

['Piyanova|Anastasia|A|', 'Lomazzo|Ermelinda|E|', 'Bindila|Laura|L|', 'Lerner|Raissa|R|', 'Albayram|Onder|O|', 'Ruhl|Tim|T|', 'Lutz|Beat|B|', 'Zimmer|Andreas|A|', 'Bilkei-Gorzo|Andras|A|']

2015

31029402

Does spontaneous adverse drug reactions' reporting differ between different reporters? A study in Toulouse Pharmacovigilance Centre.

INTRODUCTION In France since 2011, report of adverse drug reactions (ADRs) has been extended to patients (and patients' associations) who can declare directly ADRs to their regional pharmacovigilance centre. In pharmacovigilance, informativeness of ADRs reports is important to improve signal's detection. The present study was performed to compare the quality of patients', physicians and community pharmacists' reports. METHODS We performed a retrospective study investigating the quality of patients', physicians and community pharmacies' ADRs reported to Toulouse University PharmacoVigilance Centre (TUPVC) from January 2014 to June 2017. We used mandatory and non-mandatory criteria, as defined by European Medicines Agency. Reports' quality was defined as "satisfactory" when more than 90% of items were completed. We also compared reports' quality according to ADRs seriousness and the used reporting tools (email or the mobile app VigiBip®). RESULTS The number of reports to TUPVC increased between 2014 and 2016 (+51%) for patients and remained stable for pharmacists and physicians. According to the mandatory criteria, quality of the investigated reports was "satisfactory" (more than 90% of the items filled) whatever the reporter and without significant differences between reporters. For the non-mandatory criteria, clinical description of ADRs and ADRs' outcome were only filled over 90%. Significant differences were observed between the different reporters: community pharmacists informed better clinical description, ADR outcome and concomitant drugs versus both patients and physicians. Physicians informed better medical history and biological data whereas patients informed medical history and other aetiologies better than pharmacists and clinical description of ADRs better than physicians. CONCLUSION The present study failed to show differences between pharmacies', physicians' and patients' ADRs reports, for the mandatory criteria. However, significant differences were found for non-mandatory criteria with drug data more filled by pharmacists and medical ones more by physicians and patients.

['Nicol|Carole|C|', 'Moulis|Florence|F|', 'Bondon-Guitton|Emmanuelle|E|', 'Durrieu|Geneviève|G|', 'Montastruc|Jean-Louis|JL|', 'Bagheri|Haleh|H|']

2019

28440015

Hypertriglyceridemia during asparaginase treatment in children with acute lymphoblastic leukemia correlates with antithrombin activity in adolescents.

BACKGROUND Asparaginase (ASP) is a cornerstone in the treatment of acute lymphoblastic leukemia (ALL). It is also known for its ability to cause side effects, such as allergy and pancreatitis, as well as lipid and coagulation disturbances. The most important laboratory abnormalities are hypertriglyceridemia (HTG) and low antithrombin (AT). HTG is usually considered to be transient and benign in children with ALL, whereas low AT activity predisposes to thrombosis. Studies on the incidence and significance of HTG in children with ALL are scarce, and their findings have not always been congruent. We investigated the incidence and significance of ASP-related HTG, defined as triglyceride values more than five times the upper normal limit, in children with ALL. PROCEDURE We analyzed the laboratory and clinical data of children diagnosed with ALL at the Karolinska Hospital, Stockholm, Sweden, from July 2008 to December 2014. Triglyceride and AT values were measured before each injection of pegylated ASP. RESULTS The study group comprised of 92 patients, aged 1-17.9 years at diagnosis (median 4.8 years), almost half (42/92, 46%) of whom had HTG. A significant negative correlation between triglyceride and AT values was observed in those aged over 10 years (P = 0.0002). No significant correlation was found between HTG and thrombosis, osteonecrosis, or pancreatitis. CONCLUSIONS Although common, ASP-associated HTG was not associated with other ASP-related toxicities. HTG correlated with decreased AT activity in older children, which may explain previous association between HTG and thrombosis. Larger studies are of interest with regard to establishing guidelines for HGT management.

['Persson|Lisa|L|', 'Harila-Saari|Arja|A|', 'Hed Myrberg|Ida|I|', 'Heyman|Mats|M|', 'Nilsson|Anna|A|', 'Ranta|Susanna|S|']

2017

34858288

Distance Learning During the First Lockdown: Impact on the Family and Its Effect on Students' Engagement.

This contribute investigates how Emergency Remote Education (ERE) impacted families during the spring 2020 Covid-19 lockdown, and in particular, the extent to which the impact of ERE on families, measured in terms of space and equipment sharing, moderates the effect of student and family characteristics on students' engagement. The study derived from the administration of an online survey to 19,527 families with children attending schools, from nursery to upper secondary grade. The total number of student records collected amounted to 31,805, since parents had to provide data for each school-age child in the family. The survey contains 58 questions, divided into three sections, with the first two sections designed to get a reading at family level and the third section to gather data for each school-age child in the family. After verifying the validity of the engagement construct through confirmatory factor analysis, two structural equation models were used to analyze the students' engagement. The main findings reveal how the impact of the ERE on the families has had a significant role in predicting students' level of engagement observed by parents with respect to different predictor variables. Finally, we argue that it is necessary to follow a holistic approach to observe the challenges imposed by the switch of the process of deferring teaching from presence to distance, imposed by the pandemic emergency on families. In fact, a holistic approach can promote student engagement and prevent the onset of cognitive-behavioral and affective problems linked to disengagement in ERE.

['Chifari|Antonella|A|', 'Allegra|Mario|M|', 'Benigno|Vincenza|V|', 'Caruso|Giovanni|G|', 'Fulantelli|Giovanni|G|', 'Gentile|Manuel|M|', 'Ferlino|Lucia|L|']

2021

34412922

Vaccine innovation prioritisation strategy: Findings from three country-stakeholder consultations on vaccine product innovations.

As part of the Vaccine Innovation Prioritisation Strategy (VIPS), three immunization-stakeholder consultations were conducted between September 2018 and February 2020 to ensure that countries' needs drove the prioritization of vaccine product innovations. All consultations targeted respondents with immunization program experience. They included: (1) an online survey to identify immunization implementation barriers and desired vaccine attributes in three use settings, (2) an online survey to identify and evaluate the most important immunization challenges for ten exemplar vaccines, and (3) in-depth interviews to better understand the perceived programmatic benefits and challenges that could be addressed by nine innovations and to rank the innovations that could best address current challenges. The first consultation included responses from 442 participants in 61 countries, representing 89% of the 496 respondents who correctly completed at least one section of the online survey. For facility-based settings, missed opportunities for vaccination due to reluctance to open multidose vaccine vials was the barrier most frequently selected by respondents. In community-based (outreach) and campaign settings, limited access to immunization services due to geographic barriers was most frequently selected. Multidose presentations with preservative or single-dose presentations were most frequently selected as desired vaccine attributes for facility-based settings while improved thermostability was most frequently selected for outreach and campaign settings. The second online survey was completed by 220 respondents in 54 countries. For the exemplar vaccines, vaccine ineffectiveness or wastage due to heat or freeze exposure and missed opportunities due to multidose vial presentations were identified as the greatest vaccine-specific challenges. In-depth interviews with 84 respondents in six countries ranked microarray patches, dual-chamber delivery devices, and heat-stable/controlled temperature chain qualified liquid vaccines as the three innovations that could have the greatest impact in helping address current immunization program challenges. These findings informed the VIPS prioritization and provided broader application to designing immunization interventions to better meet country needs.

['Mvundura|Mercy|M|', 'Frivold|Collrane|C|', 'Janik Osborne|Anna|A|', 'Soni|Priyanka|P|', 'Robertson|Joanie|J|', 'Kumar|Sandeep|S|', 'Anena|Jacqueline|J|', 'Gueye|Abdoulaye|A|', 'Menozzi-Arnaud|Marion|M|', 'Giersing|Birgitte|B|', 'Kahn|Anna-Lea|AL|', 'Scarna|Tiziana|T|', 'Kristensen|Debra|D|']

2021

32398080

In vitro analysis of the effect of Flightless I on murine tenocyte cellular functions.

BACKGROUND Healing of tendons after injury involves the proliferation of tenocytes and the production of extracellular matrix; however, their capacity to heal is limited by poor cell density and limited growth factor activity. Flightless I (Flii) has previously been identified as an important regulator of cellular proliferation and migration, and the purpose of this study was to evaluate the effect of differential Flii gene expression on tenocyte function in vitro. METHODS The role of Flii on tenocyte proliferation, migration, and contraction was assessed using established assays. Tenocytes from Flii+/-, wild-type, and Flii overexpressing mice were obtained and the effect of differential Flii expression on migration, proliferation, contraction, and collagen synthesis determined in vitro. Statistical differences were determined using unpaired Student's t test and statistical outliers were identified using the Grubbs' test. RESULTS Flii overexpressing tenocytes showed significantly improved migration and proliferation as well as increased collagen I secretion. Explanted tendons from Flii overexpressing mice also showed significantly elevated tenocyte outgrowth compared to Flii+/- mice. In contrast to its role in dermal wound repair, Flii positively affects cellular processes in tendons. CONCLUSIONS These findings suggest that Flii could be a novel target for modulating tenocyte activity and improving tendon repair. This could have significant clinical implications as novel therapeutic targets for improved healing of tendon injuries are urgently needed.

['Jackson|Jessica E|JE|', 'Kopecki|Zlatko|Z|', 'Anderson|Peter J|PJ|', 'Cowin|Allison J|AJ|http://orcid.org/0000-0003-2885-2080']

2020

23850701

Equine herpesvirus 1 (EHV-1) nucleotide polymorphism determination using formalin fixed tissues in EHV-1 induced abortions and myelopathies with real-time PCR and pyrosequencing.

Equine herpesvirus-1 (EHV-1) strains with a single point mutation at the 2254 nucleotide position with a G2254 constitution within the DNA polymerase gene are associated strongly with equine myeloencephalopathies. Infections with non-neuropathogenic EHV-1 strains without the G2254 nucleotide but with an A2254 nucleotide are associated less frequently with equine neurologic disease. A retrospective study utilizing DNA extracted from formalin fixed paraffin embedded tissues was conducted with real time PCR and pyrosequencing, to determine the infecting EHV-1 strains. Infection with EHV-1 A2254 and or G2254 strain was detected with real time PCR, and was confirmed with a rapid pyrosequencing technique. Pyrosequencing was useful in at least 2 cases where real time PCR was equivocal in determining the infecting EHV-1 strain type. The strain with G2254 mutation was detected in 9.4% of 21 studied abortion cases, and in 86.6% of 15 neurologic cases.

['Tewari|Deepanker|D|', 'Del Piero|Fabio|F|', 'Cieply|Stephen|S|', 'Feria|Willard|W|', 'Acland|Helen|H|']

2013

25550553

Comparative effects between fucoxanthinol and its precursor fucoxanthin on viability and apoptosis of breast cancer cell lines MCF-7 and MDA-MB-231.

BACKGROUND/AIM We evaluated whether low doses of the natural carotenoid fucoxanthin and/or of its metabolite fucoxanthinol are effective against proliferation of estrogen-sensitive MCF-7 and estrogen-resistant MDA-MB-231 breast cancer cell lines. MATERIALS AND METHODS These cell lines were stimulated with 10 to 20 μM fucoxanthin and/or fucoxanthinol, followed by cell viability assays, Annexin V immunofluorescence to evaluate apoptosis, as well as mRNA and protein extractions for changes in nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) members' expressions and nuclear translocations. RESULTS Fucoxanthin and fucoxanthinol reduced the viability of MCF-7 and MDA-MB-231 cells in a time-dependent manner as a result of increased apoptosis. In both cell lines, modulatory actions of fucoxanthinol on members of the NF-κB pathway were more pronounced than that of fucoxanthin. CONCLUSION In MDA-MB-231 cells, fucoxanthinol reduced nuclear levels of NF-κB members' p65, p52 and RelB. Fucoxanthinol and fucoxanthin could be effective for the treatment and/or prevention of breast cancer.

['Rwigemera|Arlette|A|', 'Mamelona|Jean|J|', 'Martin|Luc J|LJ|']

2015

25389370

Dust, endotoxin, fungi, and bacteria exposure as determined by work task, season, and type of plant in a flower greenhouse.

BACKGROUND Greenhouse workers are exposed to dust, endotoxin, fungi, and bacteria potentially causing airway inflammation as well as systemic symptoms. Knowledge about determinants of exposure is a prerequisite for efficient prevention through knowledge-based reduction in exposure. The objective of this study was to assess the occupational exposure in a flower greenhouse and to investigate the impact of work tasks on the intensity and variability in exposure. METHODS Seventy-six personal full-shift exposure measurements were performed on 38 employees in a Danish flower greenhouse producing Campanula, Lavandula, Rhipsalideae, and Helleborus. The samples were gravimetrically analysed for inhalable dust. Endotoxin was assessed by the Limulus Amoebocyte Lysate test and culture-based quantification of bacteria and fungi was performed. Information on the performed tasks during sampling was extracted from the greenhouse electronic task logging system. Associations between log-transformed exposure outcomes, season, and work tasks were examined in linear mixed-effects regression with worker identity as random effect. RESULTS Measured concentrations ranged between 0.04 and 2.41mg m(-3) for inhalable dust and between 0.84 and 1097 EU m(-3) for endotoxin exposure, with the highest mean levels measured during Lavandula and Campanula handling, respectively. Personal exposure to fungi ranged between 1.8×10(2) and 3.4×10(6) colony-forming units (CFU) m(-3) and to bacteria between 1.6×10(1) and 4.2×10(5) CFU m(-3). Exposure to dust, endotoxin, fungi, and bacteria differed between seasons. Packing Lavandula, sticking, potting, and grading Rhipsalideae, and all examined tasks related to Campanula production except sticking increased dust exposure. Endotoxin exposure was increased during sticking Campanula and pinching or packing Rhipsalideae, and fungi exposure was elevated by subtasks performed in the research and development area for Campanula, and by potting, packing/dumping Campanula. Sticking and working with subtasks in the research and development area for Campanula increased bacteria exposure. CONCLUSION This study revealed moderate dust exposure levels compared to the levels observed in other greenhouse productions and other occupations with organic dust exposure such as farming. However, high exposures to bacteria and fungi were detected during selected tasks and the proposed health-based endotoxin exposure limit of 90 EU m(-3) was exceeded in 30% of the samples, which may have health implications for the employees. Exposure levels were found to vary depending on the tasks performed, and thereby results can be used to direct task-based initiatives to reduce workplace exposures.

['Thilsing|Trine|T|', 'Madsen|Anne Mette|AM|', 'Basinas|Ioannis|I|', 'Schlünssen|Vivi|V|', 'Tendal|Kira|K|', 'Bælum|Jesper|J|']

2015

33481068

High density fermentation of probiotic E. coli Nissle 1917 towards heparosan production, characterization, and modification.

Heparosan is a naturally occurring non-sulfated glycosaminoglycan. Heparosan serves as the substrate for chemoenzymatic synthesis of biopharmaceutically important heparan sulfate and heparin. Heparosan is biologically inert molecule, non-toxic, and non-immunogenic and these qualities of heparosan make it an ideal drug delivery vehicle. The critical-to-quality (CTQ) attributes for heparosan applications include composition of heparosan, absence of any unnatural moieties, and heparosan molecular weight size and unimodal distribution. Probiotic bacteria E. coli Nissle 1917 (EcN) is a natural producer of heparosan. The current work explores production of EcN heparosan and process parameters that may impact the heparosan CTQ attributes. Results show that EcN could be grown to high cell densities (OD600 160-180) in a chemically defined media. The fermentation process is successfully scaled from 5-L to 100-L bioreactor. The chemical composition of heparosan from EcN was confirmed using nuclear magnetic resonance. Results demonstrate that heparosan molecular weight distribution may be influenced by fermentation and purification conditions. Size exclusion chromatography analysis shows that the heparosan purified from fermentation broth results in bimodal distribution, and cell-free supernatant results in unimodal distribution (average molecular weight 68,000 Da). The yield of EcN-derived heparosan was 3 g/L of cell free supernatant. We further evaluated the application of Nissle 1917 heparosan for chemical modification to prepare N-sulfo heparosan (NSH), the first intermediate precursor for heparin and heparan sulfate. KEY POINTS: • High cell density fermentation, using a chemically defined fermentation media for the growth of probiotic bacteria EcN (E. coli Nissle 1917, a natural producer of heparosan) is reported. • Process parameters towards the production of monodispersed heparosan using probiotic bacteria EcN (Nissle 1917) has been explored and discussed. • The media composition and the protocol (SOPs and batch records) have been successfully transferred to contract manufacturing facilities and industrial partners.

['Datta|Payel|P|', 'Fu|Li|L|', 'Brodfuerer|Paul|P|', 'Dordick|Jonathan S|JS|', 'Linhardt|Robert J|RJ|http://orcid.org/0000-0003-2219-5833']

2021

31519112

Comparison of the Word Memory Test and the Test of Memory Malingering in detecting invalid performance in neuropsychological testing.

Given the prevalence of compensation seeking patients who exaggerate or fabricate their symptoms, the assessment of performance and symptom validity throughout testing is vital in neuropsychological evaluations. Two of the most commonly utilized performance validity tests (PVTs) are the Word Memory Test (WMT) and the Test of Memory Malingering (TOMM). While both have proven successful in detecting invalid performance, some studies suggest greater sensitivity in the WMT relative to the TOMM. To improve upon previous research, this study compared performance in individuals who completed both the WMT and TOMM during a neuropsychological evaluation. Participants included 268 cases from a clinical private practice consisting of primarily disability claimants. One-way multivariate analysis of variance (MANOVA) compared neuropsychological performance of participants who passed both PVTs (n = 198) versus those who failed the WMT but passed the TOMM (n = 70). Global suppression of neuropsychological scores was found for participants who failed the WMT but passed the TOMM, as well as more psychiatric symptoms reported on questionnaires, relative to those who passed both PVTs. These findings suggest that those passing the TOMM but failing the WMT demonstrated performance invalidity, which illustrates the WMT's enhanced sensitivity.

['Bhowmick|Chloe|C|', 'Hirst|Rayna|R|https://orcid.org/0000-0001-5586-9641', 'Green|Paul|P|']

2021

32874044

Clinical Outcomes of Silk versus Nylon Sutures for Suturing of Conjunctival Autograft in Pterygium Surgery.

PURPOSE The purpose is to compare the clinical results of using silk versus nylon sutures for conjunctival autograft suturing in pterygium surgery. METHODS In this prospective, randomized, controlled, clinical trial 50 eyes from 50 patients with primary nasal pterygium were randomized to undergo pterygium surgery with the use of either nylon sutures or silk sutures for conjunctival autograft suturing. Patients were followed up for 6 months. Main outcome measures included recurrence, postoperative discomfort according to a visual analog scale (VAS), graft hyperemia, and graft edema. RESULTS According to the results, there was no significant difference between groups regarding recurrence rate of pterygium (P = 0.72). A significant decrease in the mean VAS discomfort score from day 1 to day 14 was observed in both groups (P = 0.001); postoperative discomfort during the first 2 weeks, was not significantly different between the two groups. At 2 weeks' postoperatively, significantly greater number of nylon sutures remained on the autograft (P = 0.021), some of which were buried and could not be removed. CONCLUSION Both silk and nylon are effective suture materials for autograft suturing in pterygium surgery with similar postoperative discomfort and recurrence rate. Significantly greater number of nylon sutures remains buried on the autograft and could not be removed easily.

['Jamali|Hossein|H|', 'Abuali|Mostafa|M|', 'Khalili|Mohammad R|MR|']

2020

30979053

Molecular Cloning, Characterization, and Nutritional Regulation of Elovl6 in Large Yellow Croaker (Larimichthys crocea).

Elongation of very long chain fatty acids protein 6 (Elovl6) is a key enzyme in fatty acid synthesis, which participates in converting palmitate (C16:0) to stearate (C18:0). Although studies of Elovl6 have been carried out in mammals, the nutritional regulation of elovl6 in fish remains poorly understood. In the present study, the cloning and nutritional regulation of elovl6 were determined in large yellow croaker. Sequence and phylogenetic analysis revealed that the full-length cDNA of elovl6 was 1360 bp, including an open reading frame of 810 bp encoding a putative protein of 269 amino acid that possesses the characteristic features of Elovl proteins. The transcript level of elovl6 was significantly increased in the liver of croaker fed the diets with soybean oil (enriched with 18: 2n-6, LA) or linseed oil (enriched with 18: 3n-3, ALA) than that in croaker fed the diet with fish oil (enriched with 20: 5n-3 and 22: 6n-3). Correspondingly, the elovl6 expression in croaker's hepatocytes treated with ALA or LA was remarkably increased compared to the controls. Furthermore, the transcription factors including hepatocyte nuclear factor 1α (HNF1α), CCAAT-enhancer-binding protein β (CEBPβ), retinoid X receptor α (RXRα), and cAMP response element-binding protein 1 (CREB1) greatly enhanced promoter activity of elovl6 in large yellow croaker, and the expression of transcription factors is consistent with the changes of elovl6 expression in response to fatty acids in vivo and in vitro. In conclusion, this study revealed that elovl6 expression in large yellow croaker could be upregulated by dietary ALA or LA via the increased transcriptional expression of transcription factors including hnf1α, cebpβ, rxrα, and creb1.

['Li|Yongnan|Y|', 'Pang|Yuning|Y|', 'Xiang|Xiaojun|X|', 'Du|Jianlong|J|', 'Mai|Kangsen|K|', 'Ai|Qinghui|Q|']

2019

31084517

Interpretation and Understanding of the Dietary Guidelines for Americans Consumer Messages Among Low-Income Adults.

Objective: The objective of this study was to determine the interpretation, understanding, and implementation of the Dietary Guidelines for Americans (DGA) consumer messages among low-income adults and compare findings to perceptions of the messages for consumers by community nutrition educators.Methods In this mixed methods, cross-sectional study, a convenience sample of low-income adults (n = 98) with a child between the ages of 3 and 10 years old and nutrition educators (n = 9) were interviewed individually about the DGA messages, food-related behaviors, and barriers related to consuming fruits, vegetables, and whole grains. Interviews were audio-taped, transcribed verbatim, and analyzed using the inductive approach. Interpretation and ranking of the clarity and ease of the DGA messages by low-income adults and nutrition educators and perceptions about the messages were assessed. Descriptive statistics were conducted for demographic data and Fisher's exact tests were used to examine differences regarding the clarity and ease of the messages among low-income adults and nutrition educators.Results: According to the interview results, messages that tended to be misinterpreted most frequently were on topics such as sodium, fruit and vegetables, portions, and whole grain intake. Low-income adults and nutrition educators also differed in perceptions for the message clarity addressing whole grain servings (p = .001), avoiding oversized portions (p = .002), and comparing sodium (p < .001).Conclusions: Improvements in the DGA consumer messages are warranted to improve clarity and feasibility for low-income adults through new communication tools or strategies that complement the DGA.

['Chea|Molika|M|', 'Mobley|Amy R|AR|0000-0002-7477-942X']

2020

33736442

Immunohistochemistry and molecular genetics in the differential diagnostics of mesenchymal lesions of gastrointestinal tract.

Although, in routine practice, the differential diagnostics of mesenchymal tumors of the gastrointestinal tract is still focused mainly on the correct diagnosis of gastrointestinal stromal tumor and its further therapeutic management based on predictive diagnostics, recent progress in the development of endoscopic techniques has led to increased detection of other mesenchymal lesions, which were previously commonly neglected due to their small size or absence of symptoms requiring surgical exploration. Diagnosis of some of these lesions may be reached based on their histologic pattern alone, while others may be recognized with the use of tissue specific antibodies related to the probable lineage of differentiation of the neoplastic cells. Finally, a subset of tumors, commonly with uncertain lineage of differentiation, is defined by pathognomonic genetic alterations of neoplastic cells. Recognition of such alterations, based either on methods of molecular genetics or immunohistochemical detection of an altered protein product, enables a precise diagnosis in a growing number of these cases. However, regarding the fact that most of these alterations are not unique to a single tumor type, but are often shared by more neoplastic entities, the diagnosis must still be based on a complex diagnostic attitude, reflecting histological, immunohistochemical and molecular genetic features of the investigated tumor.

['Daumová|Magdaléna|M|', 'Vaňková|Bohuslava|B|', 'Švajdler|Marián|M|', 'Michal|Michal|M|', 'Daum|Ondřej|O|']

2020

30877471

Cerebral metastases: do size, peritumoral edema, or multiplicity predict infiltration into brain parenchyma?

BACKGROUND Brain metastases (BMs) are the most frequent malignancy of the central nervous system. Previous research suggested that some metastases show infiltrative behavior rather than sharp demarcation. We hypothesized that three magnetic resonance (MR) imaging parameters-(a) tumor size, (b) extent of peritumoral edema, and (c) presence of multiple BMs-are predictors of cellular invasion beyond the surgically identifiable tumor margins. METHODS We performed a post hoc analysis on prospectively collected data of patients with BMs. Biopsies beyond the resection margin and immunohistochemistry were performed to assess infiltration status. The three MR imaging parameters were dichotomized into diameters ≤ 30 mm ("small") and > 30 mm ("large"), amount of peritumoral edema "extended" and "limited," and "multiple BMs" and "single BMs," respectively. The association between infiltration status and imaging parameters was calculated using chi-square test. RESULTS Biopsy beyond the resection margin was performed in 77 patients; 49 (63.6%) had supramarginal infiltration and 28 patients (36.4%) showed no infiltration. Histological evidence of tumor infiltration was found in 25/41 patients with smaller lesions (61%) and in 24/36 with larger lesions (66.7%, p = 0.64), in 28/44 patients with limited (63.6%) and in 21/33 patients with extended edema (63.6%, p = 1.0), in 28/45 patients (62.2%) with single BM and in 21/32 patients (65.6%) with multiple BMs (p = 0.81). CONCLUSIONS Based on the post hoc analysis of our prospective trial data, we could not confirm the hypothesis that infiltration of brain parenchyma beyond the glial pseudocapsule is associated with the MR imaging parameters tumor size, extent of edema, or multiplicity of metastases.

['Fiss|Ingo|I|0000-0001-7062-495X', 'Hussein|Abdelhalim|A|', 'Barrantes-Freer|Alonso|A|', 'Sperling|Swetlana|S|', 'Hernandez-Duran|Silvia|S|', 'Wolfert|Christina|C|', 'Pukrop|Tobias|T|', 'Ninkovic|Milena|M|', 'Bleckmann|Annalen|A|', 'Rohde|Veit|V|', 'Mielke|Dorothee|D|', 'Schatlo|Bawarjan|B|']

2019

26011015

Association between Chronic Rhinosinusitis and Health-Related Quality of Life in Adults with Cystic Fibrosis.

RATIONALE Over the past four decades, the median age of survival has nearly doubled for individuals living with cystic fibrosis (CF). Chronic diseases such as chronic rhinosinusitis increase in prevalence with age. In the non-CF population, chronic rhinosinusitis is associated with reduced health-related quality of life. OBJECTIVES Our objectives were to determine the prevalence of chronic rhinosinusitis among adults with CF and to evaluate the impact of chronic rhinosinusitis on health-related quality of life. METHODS Individuals from a large academic teaching hospital in Vancouver, British Columbia, Canada, were eligible to participate in this cross-sectional study. Included subjects were at least 19 years of age, had a confirmed diagnosis of CF, and attended the CF clinic between September 2013 and April 2014. Participants completed a CF-specific health-related quality of life questionnaire (the Cystic Fibrosis Questionnaire-Revised for adolescents and adults over 14 years of age [CFQ-R 14+]) and underwent symptom and endoscopic assessment for diagnosis of chronic rhinosinusitis. Medical charts were reviewed for potential confounders, including sociodemographic (age, sex, and body mass index) and clinical (age at CF diagnosis, type of CF mutation, lung function, and chronic Pseudomonas aeruginosa infection) factors. Multivariable linear regression was used to model the relationship between chronic rhinosinusitis and CFQ-R 14+ domains, adjusted for potential confounders. MEASUREMENTS AND MAIN RESULTS A total of 121 individuals were contacted in the clinic, of whom 113 (93.4%) consented to participate. The prevalence of chronic rhinosinusitis was found to be 59.2% (95% confidence interval [CI], 49.6-68.2%). Sociodemographic and clinical factors were similarly distributed between chronic rhinosinusitis-positive and chronic rhinosinusitis-negative groups. Lung function, as measured by FEV1 (% predicted value), did not significantly differ between participants with versus those without chronic rhinosinusitis (mean difference, 2.0%; 95% CI, -8.1% to 13.0%). Following adjustment for sex and lung function, individuals with chronic rhinosinusitis reported significantly worse scores on the respiratory symptoms domains compared with their counterparts without chronic rhinosinusitis (regression coefficient, -3.93; 95% CI, -8.02 to 0.15). CONCLUSIONS The majority of adults with CF have evidence of concomitant chronic rhinosinusitis. Chronic rhinosinusitis is independently associated with worse respiratory symptom on the CFQ-R 14+. Chronic rhinosinusitis should be diagnosed and managed to optimize the health-related quality of life of adults with CF. Clinical trial registered with clinicaltrials.gov (NCT02003079).

['Habib|Al-Rahim R|AR|', 'Buxton|Jane A|JA|http://orcid.org/0000-0003-2295-393X', 'Singer|Joel|J|', 'Wilcox|Pearce G|PG|', 'Javer|Amin R|AR|', 'Quon|Bradley S|BS|']

2015

31749419

Noradrenergic Regulation of Hippocampus-Dependent Memory.

Neuromodulation regulates critical functions of CNS synapses, ranging from neural circuit development to high-order cognitive processes, including learning and memory. This broad scope of action is generally mediated through alterations of the strength of synaptic transmission (i.e. synaptic plasticity). Changes in synaptic strength are widely considered to be a cellular representation of learned information. Noradrenaline is a neuromodulator that is secreted throughout the brain in response to novelty or increased arousal. Once released, noradrenaline activates metabotropic receptors, initiating intracellular signaling cascades that promote enduring changes in synaptic strength and facilitate memory storage. Here, we provide an overview of noradrenergic modulation of synaptic plasticity and memory formation within mammalian neural circuits, which has broad applicability within the neurotherapeutics community. Advances in our understanding of noradrenaline in the context of these processes may provide a foundation for refining treatment strategies for multiple brain diseases, ranging from post-traumatic stress disorder to Alzheimer's Disease.

['Nguyen|Peter V|PV|', 'Connor|Steven A|SA|']

2019

28890684

Neural Correlates of Early Sound Encoding and their Relationship to Speech-in-Noise Perception.

Speech-in-noise (SIN) perception is a complex cognitive skill that affects social, vocational, and educational activities. Poor SIN ability particularly affects young and elderly populations, yet varies considerably even among healthy young adults with normal hearing. Although SIN skills are known to be influenced by top-down processes that can selectively enhance lower-level sound representations, the complementary role of feed-forward mechanisms and their relationship to musical training is poorly understood. Using a paradigm that minimizes the main top-down factors that have been implicated in SIN performance such as working memory, we aimed to better understand how robust encoding of periodicity in the auditory system (as measured by the frequency-following response) contributes to SIN perception. Using magnetoencephalograpy, we found that the strength of encoding at the fundamental frequency in the brainstem, thalamus, and cortex is correlated with SIN accuracy. The amplitude of the slower cortical P2 wave was previously also shown to be related to SIN accuracy and FFR strength; we use MEG source localization to show that the P2 wave originates in a temporal region anterior to that of the cortical FFR. We also confirm that the observed enhancements were related to the extent and timing of musicianship. These results are consistent with the hypothesis that basic feed-forward sound encoding affects SIN perception by providing better information to later processing stages, and that modifying this process may be one mechanism through which musical training might enhance the auditory networks that subserve both musical and language functions.

['Coffey|Emily B J|EBJ|', 'Chepesiuk|Alexander M P|AMP|', 'Herholz|Sibylle C|SC|', 'Baillet|Sylvain|S|', 'Zatorre|Robert J|RJ|']

2017

34150256

Research on heavy metal level and co-occurrence network in typical ecological fragile area.

Due to the special sensitivity of typical ecologically fragile areas, a series of human life, mining, and other activities have a greater impact on the environment. In this study, three coal mines in Ordos City on the Loess Plateau were selected as the study area, and the pollution levels of heavy metals in the area were studied by measuring As, Hg, Cr, Cd, Cu, Ni, and Pb in the soil of 131 sampling points. Combined with the concept of "co-occurrence network" in biology, the level of heavy metals in soil was studied using geostatistics and remote sensing databases. The results showed that the concentrations of Hg, Cr, Ni, Cu, and Pb in more than half of the sampling points were higher than the local environmental background value, but did not exceed the risk control value specified by China, indicating that human factors have a greater influence, while Cd and As elements are mainly affected Soil parent material and human factors influence. Heavy metal elements have nothing to do with clay and silt but have an obvious correlation with gravel. Cd, Pb, As and Ni, Cd, Cr are all positively correlated, and different heavy metals are in space The distribution also reflects the autocorrelation, mainly concentrated in the northeast of the TS mining area and the middle of the PS mining area.

['Zhao|Yiwei|Y|', 'Gao|Liangmin|L|', 'Zha|Fugeng|F|', 'Chen|Xiaoqing|X|', 'Zhou|Xiaofang|X|', 'Wang|Xinfu|X|', 'Chen|Yang|Y|', 'Pan|Xiangwei|X|']

2021

29779452

A Systematic Review of Reviews of the Outcome of Severe Neglect in Underresourced Childcare Institutions.

The aim of the systematic review described in this article was to determine the outcomes for individuals exposed to severe neglect in congregate care institutions such as orphanages. In this context, severe neglect refers to failure to meet children's basic physical, developmental, and emotional needs due to inadequate resources. In this systematic review of previous systematic reviews and meta-analyses, searches of 10 databases were conducted, 18 papers that met inclusion and exclusion criteria were selected for review, their quality was assessed, and data were extracted and synthesized. The 550 primary studies included in the 18 systematic reviews and meta-analyses were relatively well designed, allowing confidence to be placed in their results. Severe neglect was associated with a wide range of problems in the domains of physical development, cognitive development, attachment, and mental health. The severity of adverse outcomes was partly influenced by the duration and severity of deprivation and a constellation of risk and protective factors. Prevention policies should aim to eliminate large underresourced congregate care institutions for infants. In taking steps toward this, policies should aim to adequately resource congregate care institutions to meet children's developmental needs for nutrition, stimulation, and attachment to a stable primary caregiver with adequate parenting skills and training. Early placement in adoptive or foster families, with access to routine physical and mental health-care service available in developed countries, is the most viable effective intervention for child survivors of severe neglect.

['Carr|Alan|A|0000-0003-4563-8852', 'Duff|Hollie|H|', 'Craddock|Fiona|F|']

2020

34315328

Predictors of coronary artery calcification and its association with cardiovascular events in patients with chronic kidney disease.

OBJECTIVE To investigate the predictors of coronary artery calcification (CAC) and its association with cardiovascular events (CVE) in patients with stage 3-5 chronic kidney disease (CKD). METHOD Two hundred ninety CKD patients in our nephrology department were enrolled from 2018 to May 2019. The levels of matrix Gla protein (MGP) and interleukin 6 (IL-6) were measured via enzyme-linked immunosorbent assay (ELISA) method in 131 CKD patients of all. CAC was evaluated via computed tomography (CT). The covariate factors were analyzed by binary logistic regression analysis. We conducted the visits to explore the prevalence of CVE in 276 CKD patients, and covariate factors were analyzed by Cox proportional hazard model. RESULTS The prevalence of CAC was up to 57.93%. We found that age, diabetes mellitus, hyperphosphatemia, dialysis duration, and the neutrophil-lymphocyte ratio (NLR) were positively associated with CAC in all patients. In 131 patients, we demonstrated that higher IL-6 and lower MGP levels were associated with CAC. A Cox proportional hazard model demonstrated that moderate to severe CAC was correlated with an increased risk for CVE [Hazard Ratio (HR): 7.250; 95% confidence interval (CI): 3.192-16.470], and a higher MGP level was associated with a reduced risk for CVE (HR: 0.340; 95% CI: 0.124-0.933). CONCLUSIONS The prevalence of CAC in patients with CKD is a significant issue. Older age, hyperphosphatemia, dialysis duration, diabetes mellitus, IL-6, and the NLR are associated with CAC. In addition, higher MGP levels represent protective factor for CAC. Moderate to severe CAC, and lower MGP levels are associated with an increased risk for CVE. Abbreviations: AGEs: Advanced glycosylation end products; CAC: Coronary artery calcification; CACS: Coronary artery calcification score; Ca: Calcium; CI: confidence interval; CKD: Chronic kidney disease; CVE: Cardiovascular events; CT: Computed tomography; ELISA: Enzyme-linked immunosorbent assay; Hb: hemoglobin; HR: Hazard ratio; hs-CRP: high-sensitivity C-reactive protein; IL-6: Interleukin 6; iPTH: Intact parathyroid hormone; Mg: Magnesium; MGP: Matrix Gla protein; NF-κB: nuclear factor-kappa gene binding; NRL: Neutrophil-lymphocyte ratio; Runx2: Runt-related transcription factor 2; RRT: Renal replacement therapy; P: Phosphorus; Scr: Serum creatinine; TNF--alpha: Tumor necrosis factor--alpha; TC: Total cholesterol; TG: Triglyceride; VSMC: vascular smooth muscle cel.

['Wang|Xue-Rong|XR|', 'Yuan|Liang-|L|', 'Shi|Rui-|R|', 'Li|Huan-|H|', 'Wang|De-Guang|DG|', 'Wu|Yong-Gui|YG|']

2021

25395863

Clinical outcome of advanced and metastatic renal cell carcinoma treated with targeted therapy: is there a difference between young and old patients?

BACKGROUND To assess whether the clinical outcome of advanced and metastatic renal cell carcinoma (mRCC) treated with targeted therapy differs between young and old patients. PATIENTS AND METHODS A total of 327 patients with advanced renal cell carcinoma and mRCC who received targeted therapy in two Chinese clinical centers were analyzed retrospectively. The patients were stratified into three groups: young (aged <45 years), middle-aged (aged 45-64 years), and old (aged ≥65 years). Overall survival (OS) and progression-free survival (PFS) curves were drawn using the Kaplan-Meier method, and Cox's proportional hazard regression model was used to compare OS and PFS within age groups. RESULTS There were no significant differences among young, middle-aged, and old groups in terms of OS (P=0.087), whereas PFS in the old group was significantly better than in the young and middle-aged groups (P=0.043). Both OS and PFS in the younger groups (aged <65 years) were significantly worse than in the old group (age ≥65 years; median OS, 28.1 vs 28.7 months [P=0.029]; median PFS, 11.4 vs 14 months [P=0.015]). No difference in OS or PFS was found between the young and middle-aged groups. After adjusting for sex, body mass index, smoking status, hypertension, diabetes mellitus, Eastern Cooperative Oncology Group score, history of cytokines, and Fuhrman grade, old age was an independent favorable prognostic factor for OS and PFS compared with younger age (<65 years) (OS, hazard ratio, 0.552 [95% confidence interval, 0.329-0.828; P=0.006]; PFS, hazard ratio, 0.584 [95% confidence interval, 0.401-0.850; P=0.005]). CONCLUSION Younger patients with advanced renal cell carcinoma and mRCC receiving targeted therapy have a poorer prognosis compared with old patients. These results remain to be examined in prospective cohorts.

['Zhang|Guiming|G|', 'Zhu|Yao|Y|', 'Dong|Dahai|D|', 'Gu|Weijie|W|', 'Zhang|Hailiang|H|', 'Sun|Lijiang|L|', 'Ye|Dingwei|D|']

2014

34269261

Preoperative mitral annulus size - Can we get it right?

Objective We looked for a correlation between the surgically measured mitral valve size and the cardiac dimensions (left ventricle internal diameter, left atrial size, aorta size, and body surface area) measured by preoperative and intraoperative echocardiography. We also assessed to see if we could predict the mitral prosthesis size based on the correlation data obtained. Methods The hospital records of 180 patients who underwent mitral valve replacement (MVR) with TTK Chitra valve between January 2008 and December 2012 at our hospital, were studied. The correlation between surgically measured mitral annulus size to left ventricular internal diameter systolic (LVIDS) and diastolic (LVIDD), left atrial size (linear measurement), and aorta size on echocardiography and body surface area was calculated using Pearson correlation coefficient. Mean LVIDS was calculated for each valve prosthesis size separately and the correlation was studied. Results The correlation between mitral valve prosthesis size and left ventricular internal diameter (systolic) showed a Pearson coefficient of 3.3 with significance at the level 0.01. Mitral valve size and left atrial size showed a correlation coefficient of 2.7 with significance at the level 0.01. The correlation coefficient for mitral valve size with left ventricular internal diameter diastolic, aorta and body surface area were 2.5, 1.9, and 1.8, respectively. There was a gradual increase in the mean LVIDS with increase in the prosthetic valve size. Box plot and scatter plot showed linear correlation between valve size and mean LVIDS.

['Panicker|Varghese T|VT|', 'Sreekantan|Renjith|R|', 'Lokanath|Nagananda|N|']

2021

33432391

A Modified Superior Pedicle Mastopexy Technique With an Inferolateral-Based Auto Augmentation Flap.

BACKGROUND Current mastopexy techniques have evolved to decrease scar length and maintain a more consistent upper pole fullness, improving the breast shape. Many different approaches have tried to suspend breast tissues to achieve a more attractive upper pole. Most of the auto-augmentation mastopexy techniques use inferior-based breast parenchymal tissues to fill the upper part. METHODS This paper presents a modified approach to fill the breast's upper pole, with an inferolateral-based breast flap. The advantages of changing the inferior-based auto-augmentation technique to the inferolateral comprise improving blood supply and increasing repositioned breast flap's mobility. In our technique, the breast tissue used to auto-augment the upper pole receives its blood supply from pectoral perforators and lateral breast tissue. When surgeons need more flap mobility, they can raise the medial edge of this flap from the pectoral fascia to mobilize this flap higher on the chest wall, depending on the lateral blood supply. One other advantage is that when the inferolateral-based breast tissue is pulled in a superomedial direction to fill the upper part, the lateral ptotic breast tissues come closer to the breast meridian to meet with medial breast parenchyma easily. The tension on the vertical parenchymal suture line is reduced with this approach. CONCLUSION Our technique can be used in most mastopexy operations and for all types of ptosis except for cases with insufficient breast volume. It brings safety to the auto-augmentation procedure and is also time-saving. The stability of breast shape and upper pole fullness lasts as long as other auto-augmentation procedures; therefore, it can be an excellent alternative to other techniques. LEVEL OF EVIDENCE IV This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

['Yilmaz|Kahraman Berkhan|KB|http://orcid.org/0000-0001-5042-5857']

2021

30044419

Microstructures and Compressive Properties of Al Matrix Composites Reinforced with Bimodal Hybrid In-Situ Nano-/Micro-Sized TiC Particles.

Bimodal hybrid in-situ nano-/micro-size TiC/Al composites were prepared with combustion synthesis of Al-Ti-C system and hot press consolidation. Attempt was made to obtain in-situ bimodal-size TiC particle reinforced dense Al matrix composites by using different carbon sources in the reaction process of hot pressing forming. Microstructure showed that the obtained composites exhibited reasonable bimodal-sized TiC distribution in the matrix and low porosity. With the increasing of the carbon nano tube (CNT) content from 0 to 100 wt. %, the average size of the TiC particles decreases and the compressive strength of the composite increase; while the fracture strain increases first and then decreases. The compressive properties of the bimodal-sized TiC/Al composites, especially the bimodal-sized composite synthesized by Al-Ti-C with 50 wt. % CNTs as carbon source, were improved compared with the composites reinforced with single sized TiC. The strengthening mechanism of the in-situ bimodal-sized particle reinforced aluminum matrix composites was revealed.

['Qiu|Feng|F|0000-0003-4741-7906', 'Tong|Hao-Tian|HT|', 'Gao|Yu-Yang|YY|', 'Zou|Qian|Q|', 'Dong|Bai-Xin|BX|', 'Li|Qiang|Q|', 'Chu|Jian-Ge|JG|', 'Chang|Fang|F|', 'Shu|Shi-Li|SL|', 'Jiang|Qi-Chuan|QC|']

2018

27031296

Biotic and abiotic controls on nitrogen leaching losses into waterways during successive bovine urine application to soil.

Cattle waste products high in nitrogen (N) that enter waterways via rainfall runoff can contribute to aquatic ecosystem health deterioration. It is well established that N leaching from this source can be reduced by plant assimilation, e.g. pasture grass. Additionally, N leaching can be reduced when there is sufficient carbon (C) in the soil such as plant litterfall to stimulate microbial processes, i.e. denitrification, which off-gas N from the soil profile. However, the relative importance of these two processes is not well understood. A soil microcosm experiment was conducted to determine the role of biotic processes, pasture grass and microbial activity, and abiotic processes such as soil sorption, in reducing N leaching loss, during successive additions of bovine urine. Pasture grass was the most effective soil cover in reducing N leaching losses, which leached 70% less N compared to exposed soil. Successive application of urine to the soil resulted in N accumulation, after which there was a breaking point indicated by high N leaching losses. This is likely to be due to the low C:N ratio within the soil profiles treated with urine (molar ratio 8:1) compared to water treated soils (30:1). In this experiment we examined the role of C addition in reducing N losses and showed that the addition of glucose can temporarily reduce N leaching. Overall, our results demonstrated that plant uptake of N was a more important process in preventing N leaching than microbial processes.

['Neilen|Amanda D|AD|', 'Chen|Chengrong R|CR|', 'Faggotter|Stephen J|SJ|', 'Ellison|Tanya L|TL|', 'Burford|Michele A|MA|']

2016

34518449

What's New with Endoscopic Treatments for Early Gastric Cancer in the "Post-ESD Era"?

BACKGROUND Endoscopic treatments for gastric cancers have still been progressing even after the great success of endoscopic submucosal dissection (ESD). SUMMARY In further advancements of ESD, safe and less-invasive procedures are challenged by managing postoperative bleeding, one of the major adverse events in ESD. Covering the mucosal defect after removal of lesions appears reasonable and effective for preventing delayed bleeding from the post-ESD ulcers. Shielding with biodegradable sheets is attempted on clinical trials, which show equivocal results. Although suturing of the mucosal rims is technically challenging, pilot studies demonstrate favorable outcomes for avoiding post-ESD bleeding even in cases at high risk. In cases after noncurative resection of ESD, the selection of patients who truly require additional gastrectomy with lymph node dissection is important to provide necessary surgery. Risk stratification of lymph node metastases and surgery has been developed, which offers tailor-made management to each patient considering the risks and benefits. In surgery, function-preserving gastrectomy to minimize the resection area in both lymphadenectomy and the primary site is clinically introduced. The sentinel node navigation surgery is promising to realize the minimally invasive gastrectomy, and it should strongly fit ESD as well as laparoscopic endoscopic cooperative surgery or endoscopic full-thickness resection, although nonexposure approaches are desirable. Key Message: Development for less-invasive managements on gastric cancer will be continued in step with the advancement of endoscopic treatments.

['Goto|Osamu|O|', 'Kaise|Mitsuru|M|', 'Iwakiri|Katsuhiko|K|']

2021

31196742

LncRNA TRG-AS1 promotes glioblastoma cell proliferation by competitively binding with miR-877-5p to regulate SUZ12 expression.

Glioblastoma is one of the most fatal diseases in human central nerve system. However, the prognosis and treatment of glioblastoma still call for steady improvement. In recent years, increasing studies have revealed that the abnormal expression of long non-coding RNA (lncRNA) is closely related to carcinogenesis and prognosis. Unfortunately, many lncRNAs still need further research in their function and molecule mechanism. LncRNA TRG-AS1 hasn't been detected in any types of cancers before. TRG-AS1 is associated with poor prognosis and is upregulated in glioblastoma tissues and cells. TRG-AS1 can also accelerate glioblastoma cell proliferation in return. On the other hand, miRNA-877-5p expresses low in glioblastoma and contains binding sites with both TRG-AS1 and SUZ12. Furthermore, TRG-AS1 suppresses the expression of miR-877-5p while miR-877-5p suppresses SUZ12 expression. Overexpression of TRG-AS1 could promote the expression of SUZ12.Rescue assays demonstrates that overexpression of SUZ12 can counteract the decline of glioblastoma cell proliferation induced by knockdown of TRG-AS1. Based on all these assays, TRG-AS1 promotes glioblastoma cell proliferation by acting as a ceRNA of miR-877-5p to regulate SUZ12 expression. TRG-AS1 might serve as a new target in glioblastoma treatment.

['Xie|Haishu|H|', 'Shi|Songsheng|S|', 'Chen|Quan|Q|', 'Chen|Zhen|Z|']

2019

32978083

Beliefs and Perspectives of Women and Obstetrical Providers in Northern Ontario on Water Births.

OBJECTIVE This study sought to understand the beliefs and perspectives of women in northern Ontario and their obstetrical providers with respect to water birthing as access to this service is limited in this region METHODS: All midwives, family physicians (FPs), and obstetricians providing labour and delivery services in northern Ontario were surveyed, as were a sample of labour and delivery nurses in the region and convenience samples of regional women. RESULTS Of the 362 women who completed the survey (a 90.5% response rate), 81.8% (95% CI 77.5-85.4) believed water births to be safe, 40.9% (95% CI 35.9-46) were interested in having a water birth, and 76.5% (95% CI 71.8-80.5) wanted to have the option of a hospital-based water birth. Perceptions of water birth safety varied significantly by provider type (χ2P < 0.001) with 100% (95% CI 89.6-100) of midwives but 0% (95% CI 52.3-94.9) of obstetricians considering them to be safe. Perceptions of the specific risks and benefits of water birth also varied significantly by provider type, as did understanding of consumer interest. Reflecting these perceptions, 97.1% (95% CI 85.1-99.5) of midwives and 0% (95% CI 0-27.8) of obstetricians would consider assisting in or providing hospital-based water births. CONCLUSIONS Women in northern Ontario are interested in water birth and in having this service available in hospitals. However, given the widely divergent views of the professional groups providing labour and delivery care in the region, hospitals should be strongly encouraged to explore interprofessional development opportunities to enable patient-centred care in this context.

['Orrantia|Eliseo|E|', 'Petrick|Carmen|C|']

2021

31332620

Association between snoring and heart rate variability in adolescents: effects of gender and insufficient sleep.

PURPOSE We explored the association between subjective snoring frequency and heart rate variability (HRV) in a healthy adolescent population. METHODS A total of 102 healthy adolescents were recruited from high schools in South Korea, and reported their sleep habits and snoring frequency. HRV was assessed to indirectly measure autonomic function. We assessed correlations between snoring frequency and HRV indices. We also investigated the effects of sex and behaviorally induced insufficient sleep syndrome (BISS) on the associations between HRV parameters and snoring frequency. RESULTS Overall, significant correlations were apparent between snoring frequency and HRV indices including the standard deviation of the normal-to-normal intervals (SDNN) and the low-frequency/high-frequency (LF/HF) ratio. Associations were more evident in males and those with BISS compared to females and those without BISS. CONCLUSIONS Our findings suggest that snoring changes autonomic nervous system (ANS) activity in adolescents; the changes are more dramatic in males and those with insufficient sleep.

['Jeon|Sehyun|S|', 'Oh|Seongmin|S|', 'Cho|Seong-Jin|SJ|', 'Lee|Yu Jin|YJ|', 'Kim|Seog Ju|SJ|']

2020

33288169

Effects of drying techniques on the physicochemical, functional, thermal, structural and rheological properties of mung bean (Vigna radiata) protein isolate powder.

Mung bean is an inexpensive yet sustainable protein source. Current work compared the effects of freeze (FD), spray (SD) and oven drying (OD), on mung bean protein isolate (MBPI) produced on pilot scale. All samples showed no dissociation of protein subunits and were thermally stable (Td = 157.90-158.07 °C). According to morphological studies, FD formed a porous protein while SD and OD formed wrinkled and compact crystals, respectively. FD and SD formed elastic gels with better gelling capacity than OD (aggregated gel). FD showed exceptional protein solubility, water and oil absorption capacity (4.23 g/g and 8.38 g/g, respectively). SD demonstrated the smallest particle size, excellent emulsion activity index (29.21 m2/g) and stability (351.90 min) and the highest β-sheet amount (37.61%). FTIR spectra for all samples showed characteristic peaks which corresponded well to the secondary structure of legume proteins. Rheological analysis revealed that gelation temperature for all MBPI lied around 90 °C. Current work described the different final properties achieved for MBPI produced under different drying techniques that allowed tailoring for different food systems, whereby FD is ideal for meat extender, SD is suitable for meat emulsion while OD is suitable in general protein-based application.

['Brishti|Fatema Hossain|FH|', 'Chay|Shyan Yea|SY|', 'Muhammad|Kharidah|K|', 'Ismail-Fitry|Mohammad Rashedi|MR|', 'Zarei|Mohammad|M|', 'Karthikeyan|Sivakumaran|S|', 'Saari|Nazamid|N|']

2020

30791824

The Effect of Atosiban on Patients With Difficult Embryo Transfers Undergoing In Vitro Fertilization-Embryo Transfer.

The purpose of this study was to evaluate the effect of atosiban on the outcomes of infertile women undergoing in vitro fertilization (IVF) with difficult embryo transfers (ETs). This randomized double-blind study enrolled 204 infertile women with difficult ETs during IVF treatment between June 2014 and June 2018. According to a computer-generated randomization list, participants were randomized into placebo (n = 102) and atosiban (n = 102) groups. In atosiban group, atosiban with a total dose of 37.5 mg was administered. All of the patients underwent IVF-ET using cryopreserved embryos. The clinical pregnancy rate per cycle and implantation rate per transfer (45.1% and 26.5%) in atosiban group were significantly higher than those of placebo group (15.6% and 9.7%, respectively; P < .05). This study showed that administration of atosiban during ET was extraordinarily effective for patients with difficult transfers.

['Yuan|Caixia|C|', 'Song|Haixia|H|', 'Fan|Lingling|L|', 'Su|Shili|S|', 'Dong|Baihua|B|']

2019

28531445

Danish evidence-based clinical guideline for use of nutritional support in pulmonary rehabilitation of undernourished patients with stable COPD.

BACKGROUND AND AIMS Disease-related under-nutrition is a common problem in individuals with COPD. The rationale for nutritional support in pulmonary rehabilitation therefore seems obvious. However there is limited evidence regarding the patient-relevant outcomes i.e. activities of daily living (ADL) or quality of life. Therefore the topic was included in The Danish Health and Medicines Authority's development of an evidence-based clinical guideline for rehabilitation of patients with stable COPD. METHODS The methods were specified by The Danish Health and Medicines Authority as part of a standardized approach to evidence-based national clinical practice guidelines. They included formulation of a PICO with pre-defined criteria for the Population, Intervention, Control and Outcomes. Existing guidelines or systematic reviews were used after assessment using the AGREE II tool or AMSTAR, if possible. We identified primary studies by means of a systematic literature search (July to December 2013), and any identified studies were then quality assessed using the Cochrane risk of bias tool and the GRADE approach. The extracted data on our pre-defined outcomes were summarized in meta-analyses when possible, or meta-analyses from existing guidelines or systematic reviews were adapted. The results were used for labeling and wording of the recommendations. RESULTS Data from 12 randomized controlled trials were included in a systematic review, which formed the basis for our recommendations as no new primary studies had been published. There were evidence of moderate quality that nutritional support for undernourished patients with COPD lead to a weight gain of 1.7kg (95% confidence interval: 1.3 to 2.2kg), but the effect was quantified as a mean change from baseline, which is less reliable. There were evidence of moderate quality that nutritional therapy does not increase in the 6 minute walking distance of 13 m (95% confidence interval: -27 to 54 m) when results in the intervention and control groups were compared at 9-16 weeks of follow-up. There was evidence of very low quality for an increase in lean body mass. The studies did not demonstrate an effect on either quality of life or ADL in patients with COPD. Some pre-defined outcomes (adverse events, hospital admissions and mortality) were not quantified. CONCLUSION The evidence base for nutritional supplementation in rehabilitation of COPD patients is weak and any effect was limited to surrogate markers, such as increased weight and lean body mass, while an effect could not be seen on patient-relevant outcomes such as quality of life or activities of daily living. The intervention was given a weak recommendation.

['Beck|Anne Marie|AM|', 'Iepsen|Ulrik Winning|UW|', 'Tobberup|Randi|R|', 'Jørgensen|Karsten Juhl|KJ|']

2015

34502869

Langasite as Piezoelectric Substrate for Sensors in Harsh Environments: Investigation of Surface Degradation under High-Temperature Air Atmosphere.

Langasite crystals (LGS) are known for their exceptional piezoelectric properties at high temperatures up to 1000 °C and more. In this respect, many studies have been conducted in order to achieve surface acoustic wave (SAW) sensors based on LGS crystals dedicated to high-temperature operations. Operating temperatures of more than 1000 °C and 600 °C for wired and wireless sensors, respectively, have been reached. These outstanding performances have been obtained under an air atmosphere since LGS crystals are not stable in high-temperature conditions under a low-oxygen atmosphere due to their oxide nature. However, if the stability of bulk LGS crystals under a high-temperature air atmosphere is well established, the surface deterioration under such conditions has been hardly investigated, as most of the papers dedicated to LGS-based SAW sensors are essentially focused on the development of thin film electrodes that are able to withstand very elevated temperatures to be combined with LGS crystals. Yet, any surface modification of the substrate can dramatically change the performance of SAW sensors. Consequently, the aim of this paper is to study the stability of the LGS surface under a high-temperature air environment. To do so, LGS substrates have been annealed in an air atmosphere at temperatures between 800 and 1200 °C and for durations between one week and one month. The morphology, microstructure, and chemical composition of the LGS surface was examined before and after annealing treatments by numerous and complementary methods, while the surface acoustic properties have been probed by SAW measurements. These investigations reveal that depending on both the temperature and the annealing duration, many defects with a corolla-like shape appear at the surface of LGS crystals in high-temperature prolonged exposure in an air atmosphere. These defects are related to the formation of a new phase, likely an oxiapatite ternary compound, the chemical formula of which is La14GaxSi9-xO39-x/2. These defects are located on the surface and penetrate into the depth of the sample by no more than 1-2 microns. However, SAW measurements show that the surface acoustic properties are modified by the high-temperature exposure at a larger deepness of at least several tens of microns. These perturbations of the LGS surface acoustic properties could induce, in the case of LGS-based SAW sensors operating in the 434 MHz ISM band, temperature measurement errors around 10 °C.

['Aubert|Thierry|T|', 'Kokanyan|Ninel|N|0000-0003-2625-2671', 'Elmazria|Omar|O|0000-0003-4917-9120']

2021

31215101

Metabolomic profile as a noninvasive adjunct tool for the diagnosis of Grades III and IV endometriosis-related infertility.

The aim of the present case-control study was to develop a noninvasive adjuvant tool for the diagnosis of endometriosis. Serum samples from 100 patients undergoing intracytoplasmic sperm injection were split into two groups according to the cause of infertility: an endometriosis group (n = 50), consisting of samples derived from patients with Grade III and IV endometriosis, and a control group (n = 50), comprising samples derived from patients with isolated male factor infertility. The metabolomic profile of each sample was obtained, through mass spectrometry. Partial least squares discriminant analysis was able to clearly classify the endometriosis and control groups. Ten potential biomarkers were selected based on their importance for model prediction. These ions were used to build the receiver-operating characteristic curve, which presented an area under the curve of 0.904 (95% confidence interval: 0.796-0.985). To validate the model, 30 other samples from infertile women without any evidence of endometriosis were tested. Considering these ions as possible biomarkers, the model was able to correctly classify 84% of the patients. Finally, a similar prediction potential was observed in the model validated set, when samples from the disease-free group were tested. Serum metabolomics may be useful as a noninvasive adjunct tool for the selection of patients who must undergo laparoscopy for definitive endometriosis diagnosis.

['Braga|Daniela P A F|DPAF|0000-0003-1333-6593', 'Montani|Daniela A|DA|0000-0002-0637-6848', 'Setti|Amanda S|AS|', 'Turco|Edson G Lo|EGL|', 'Oliveira-Silva|Diogo|D|0000-0002-0046-3502', 'Borges|Edson|E|']

2019

26619125

Interventions to increase seasonal influenza vaccine coverage in healthcare workers: A systematic review and meta-regression analysis.

Influenza vaccination is recommended for healthcare workers (HCWs), but coverage is often low. We reviewed studies evaluating interventions to increase seasonal influenza vaccination coverage in HCWs, including a meta-regression analysis to quantify the effect of each component. Fourty-six eligible studies were identified. Domains conferring a high risk of bias were identified in most studies. Mandatory vaccination was the most effective intervention component (Risk Ratio of being unvaccinated [RRunvacc] = 0.18, 95% CI: 0.08-0.45), followed by "soft" mandates such as declination statements (RRunvacc = 0.64, 95% CI: 0.45-0.92), increased awareness (RRunvacc = 0.83, 95% CI: 0.71-0.97) and increased access (RRunvacc = 0.88, 95% CI: 0.78-1.00). For incentives the difference was not significant, while for education no effect was observed. Heterogeneity was substantial (τ(2) = 0.083). These results indicate that effective alternatives to mandatory HCWs influenza vaccination do exist, and need to be further explored in future studies.

['Lytras|Theodore|T|', 'Kopsachilis|Frixos|F|', 'Mouratidou|Elisavet|E|', 'Papamichail|Dimitris|D|', 'Bonovas|Stefanos|S|']

2016

34484082

Into the Unknown: Uncertainty and Procrastination in Students From a Life History Perspective.

While existing findings have established an increase in procrastination among students in higher education during COVID-19, they do not elucidate how COVID-19 has effected an increase in procrastination. Drawing upon previous findings and employing a life history framework, this paper proposed that the increase in procrastination may be attributed to the heightened levels of uncertainty in the pandemic. Additionally, this paper examined life history strategy as the psychological mechanism underlying the relation between uncertainty and procrastination. By collecting data across two school semesters in a university (N = 253), we found that uncertainty and procrastination did not differ between the semester where changes were abruptly imposed to stem the spread of COVID-19 and the following semester. Our findings also revealed that uncertainty predicted procrastination, and that life history strategy mediated the relation between uncertainty and procrastination. Specifically, uncertainty was associated with a faster life history strategy, which was positively associated with procrastination. By shedding light on the psychology behind the effect of uncertainty on procrastination, the findings of this paper hold important implications for the design of procrastination interventions for the uncertain climate during the pandemic and "the new normal" post COVID-19.

['Lim|Amy J|AJ|', 'Javadpour|Sasha|S|']

2021

27318809

Using the PDD Behavior Inventory as a Level 2 Screener: A Classification and Regression Trees Analysis.

In order to improve discrimination accuracy between Autism Spectrum Disorder (ASD) and similar neurodevelopmental disorders, a data mining procedure, Classification and Regression Trees (CART), was used on a large multi-site sample of PDD Behavior Inventory (PDDBI) forms on children with and without ASD. Discrimination accuracy exceeded 80 %, generalized to an independent validation set, and generalized across age groups and sites, and agreed well with ADOS classifications. Parent PDDBIs yielded better results than teacher PDDBIs but, when CART predictions agreed across informants, sensitivity increased. Results also revealed three subtypes of ASD: minimally verbal, verbal, and atypical; and two, relatively common subtypes of non-ASD children: social pragmatic problems and good social skills. These subgroups corresponded to differences in behavior profiles and associated bio-medical findings.

['Cohen|Ira L|IL|0000-0001-6688-0141', 'Liu|Xudong|X|', 'Hudson|Melissa|M|', 'Gillis|Jennifer|J|', 'Cavalari|Rachel N S|RN|', 'Romanczyk|Raymond G|RG|', 'Karmel|Bernard Z|BZ|', 'Gardner|Judith M|JM|']

2016

33584755

Genetic Basis of Gluten Aggregation Properties in Wheat (Triticum aestivum L.) Dissected by QTL Mapping of GlutoPeak Parameters.

Bread wheat is one of the most important crops worldwide, supplying approximately one-fifth of the daily protein and the calories for human consumption. Gluten aggregation properties play important roles in determining the processing quality of wheat (Triticum aestivum L.) products. Nevertheless, the genetic basis of gluten aggregation properties has not been reported so far. In this study, a recombinant inbred line (RIL) population derived from the cross between Luozhen No. 1 and Zhengyumai 9987 was used to identify quantitative trait loci (QTL) underlying gluten aggregation properties with GlutoPeak parameters. A linkage map was constructed based on 8,518 SNPs genotyped by specific length amplified fragment sequencing (SLAF-seq). A total of 33 additive QTLs on 14 chromosomes were detected by genome-wide composite interval mapping (GCIM), four of which accounted for more than 10% of the phenotypic variation across three environments. Two major QTL clusters were identified on chromosomes 1DS and 1DL. A premature termination of codon (PTC) mutation in the candidate gene (TraesCS1D02G009900) of the QTL cluster on 1DS was detected between Luozhen No. 1 and Zhengyumai 9987, which might be responsible for the difference in gluten aggregation properties between the two varieties. Subsequently, two KASP markers were designed based on SNPs in stringent linkage with the two major QTL clusters. Results of this study provide new insights into the genetic architecture of gluten aggregation properties in wheat, which are helpful for future improvement of the processing quality in wheat breeding.

['Zhou|Zhengfu|Z|', 'Zhang|Ziwei|Z|', 'Jia|Lihua|L|', 'Qiu|Hongxia|H|', 'Guan|Huiyue|H|', 'Liu|Congcong|C|', 'Qin|Maomao|M|', 'Wang|Yahuan|Y|', 'Li|Wenxu|W|', 'Yao|Wen|W|', 'Wu|Zhengqing|Z|', 'Tian|Baoming|B|', 'Lei|Zhensheng|Z|']

2020

28367653

Placental oxidative stress and maternal endothelial function in pregnant women with normotensive fetal growth restriction.

OBJECTIVE The purpose of this study was to investigate the relationship between placental oxidative stress and maternal endothelial function in pregnant women with normotensive fetal growth restriction (FGR). METHODS We examined serum concentrations of oxygen free radicals (d-ROMs), maternal angiogenic factor (PlGF), and sFlt-1, placental oxidative DNA damage, and maternal endothelial function in 17 women with early-onset preeclampsia (PE), 18 with late-onset PE, 14 with normotensive FGR, and 21 controls. Flow-mediated vasodilation (FMD) was assessed as a marker of maternal endothelial function. Immunohistochemical analysis was performed to measure the proportion of placental trophoblast cell nuclei staining positive for 8-hydroxy-2'-deoxyguanosine (8-OHdG), a marker of oxidative DNA damage. RESULTS Maternal serum d-ROM, sFlt-1 concentrations, and FMD did not significantly differ between the control and normotensive FGR groups. The proportion of nuclei staining positive for 8-OHdG was significantly higher in the normotensive FGR group relative to the control group. CONCLUSIONS Our findings demonstrate that, despite the presence of placental oxidative DNA damage as observed in PE patients, pregnant women with normotensive FGR show no increase in the concentrations of sFlt-1 and d-ROMs, or a decrease in FMD.

['Yoshida|Atsumi|A|', 'Watanabe|Kazushi|K|', 'Iwasaki|Ai|A|', 'Kimura|Chiharu|C|', 'Matsushita|Hiroshi|H|http://orcid.org/0000-0002-8219-9011', 'Wakatsuki|Akihiko|A|']

2018

33808053

Increasing Capacity to Detect Clusters of Rapid HIV Transmission in Varied Populations-United States.

Molecular cluster detection analyzes HIV sequences to identify rapid HIV transmission and inform public health responses. We describe changes in the capability to detect molecular clusters and in geographic variation in transmission dynamics. We examined the reporting completeness of HIV-1 polymerase sequences in quarterly National HIV Surveillance System datasets from December 2015 to December 2019. Priority clusters were identified quarterly. To understand populations recently affected by rapid transmission, we described the transmission risk and race/ethnicity of people in clusters first detected in 2018-2019. During December 2015 to December 2019, national sequence completeness increased from 26% to 45%. Of the 1212 people in the 136 clusters first detected in 2018-2019, 69% were men who have sex with men (MSM) and 11% were people who inject drugs (PWID). State-by-state analysis showed substantial variation in transmission risk and racial/ethnic groups in clusters of rapid transmission. HIV sequence reporting has increased nationwide. Molecular cluster analysis identifies rapid transmission in varied populations and identifies emerging patterns of rapid transmission in specific population groups, such as PWID, who, in 2015-2016, comprised only 1% of people in such molecular clusters. These data can guide efforts to focus, tailor, and scale up prevention and care services for these populations.

['Oster|Alexandra M|AM|', 'Panneer|Nivedha|N|', 'Lyss|Sheryl B|SB|', 'McClung|R Paul|RP|', 'Watson|Meg|M|', 'Saduvala|Neeraja|N|', 'Ocfemia|M Cheryl Bañez|MCB|', 'Linley|Laurie|L|', 'Switzer|William M|WM|0000-0003-4591-8731', 'Wertheim|Joel O|JO|', 'Campbell|Ellsworth|E|0000-0002-9535-4420', 'Hernandez|Angela L|AL|', 'France|Anne Marie|AM|']

2021

32347309

The fundamental role of the endocannabinoid system in endometrium and placenta: implications in pathophysiological aspects of uterine and pregnancy disorders.

BACKGROUND The endocannabinoid system (ECS) consists of the cannabinoid receptors CB1 and CB2, the main endocannabinoids anandamide (AEA) and 2-arachidonoylglycerol (2-AG) and their metabolic enzymes N-acylphosphatidylethanolamine-specific phospholipase D, fatty acid amide hydrolase, diacylglycerol lipase and monoacylglycerol lipase. This system is involved in the modulation of essential physiological processes. Its role in the reproductive system has become significantly important in recent years, given its major role in events such as gametogenesis, decidualisation, implantation and placentation. OBJECTIVE AND RATIONALE In this paper, we review the literature and summarize the role of the ECS elements in reproduction and their potential as early markers for diagnosis of reproductive disorders or as pharmacological targets for treatment. SEARCH METHODS Original research and review papers published from 1964 to June 2019 were selected in terms of relevance, reliability and quality by searching PubMed, MEDLINE and Web of Science, using the following search terms: endocannabinoid system and endometriosis; endocannabinoid system and ectopic pregnancy; endocannabinoid system and miscarriage; endocannabinoid system and pre-eclampsia; endocannabinoid system and endometrial cancer; endocannabinoid system and reproduction; endocannabinoid, endometrium; placenta; N-acylethanolamines; anandamide; 2-arachidonoylglycerol; and cannabinoids. OUTCOMES This review demonstrates relevant information concerning ECS alterations in endometriosis, ectopic pregnancy, miscarriage, pre-eclampsia and endometrial cancer. We highlight the importance of the endocannabinoids in endometrial and placental physiology and pathophysiology, from studies in vitro and in vivo and in clinical observations. The most studied of the endogenous cannabinoids is AEA. The levels of AEA were increased in plasma of patients with endometriosis and miscarriage, as well as in the fallopian tube of women with ectopic pregnancy and in endometrial biopsies of endometrial cancer. Changes in the pattern of expression of the cannabinoid receptor CB1 were also observed in endometrial biopsies of endometriosis, fallopian tube and decidua of patients with ectopic pregnancy and pre-eclamptic placenta. Moreover, alterations in CB2 expression have been reported in association with endometrial cancer. In general, studies on the cannabinoid signalling through CB2 and on the biological activities of the other major endocannabinoid, namely 2-AG, as well as its metabolic enzymes are scarce and avidly required. WIDER IMPLICATIONS The pathophysiological mechanisms involved in the described endometrial and placental pathologies are still unclear and lack the means for an early diagnosis. Based on current evidence, though alterations in ECS are demonstrated at tissue level, it is difficult to associate plasmatic changes in AEA with specific endometrial and placental diseases. Thus, pairing alterations in AEA levels with 2-AG and/or other endocannabinoid-like molecules may provide more accurate and early diagnoses. In addition, patients may benefit from new therapies that target the ECS and endocannabinoid signalling.

['Maia|J|J|', 'Fonseca|B M|BM|', 'Teixeira|N|N|', 'Correia-da-Silva|G|G|']

2020

34213382

Oxidative stress in corneal stromal cells contributes to the development of keratoconus in a rabbit model.

PURPOSE To investigate the role of oxidative stress in keratocytes in the pathogenesis of keratoconus (KC) using the rabbit cornea as a model. METHODS Immerse the rabbit cornea in collagenase type II solution at room temperature for 30 min in the KC group. The central cornea thickness (CCT), and mean keratometry (Km) were examined before and after the procedure. Reactive oxygen species (ROS), the nuclear translocation of nuclear factor E2-related factor 2 (NRF-2), the expression of heme oxygenase-1 (HO-1) protein, and nicotinamide adenine dinucleotide phosphate (NADPH) Oxidase (NOX) family members NOX-2 and NOX-4 protein levels were examined by immunohistochemistry analysis and Western Blot. The expression levels of HO-1, NOX-2, NOX-4, and NRF-2 mRNA were quantitatively detected by Real-time PCR. RESULTS A significant increase in Km and a significant decrease in CCT were observed in the KC group compared with the control group after the surgery (both p < 0.001). Immunofluorescence staining showed the rabbit KC model induced a significant increase in ROS production (p < 0.001). The expression of HO-1, NOX-2, NOX-4, and NRF-2 proteins in the KC group were significantly higher than those in the control group (all p < 0.001). RT-PCR results showed the levels of HO-1, NOX-2, NOX-4, and NRF-2 mRNA in KC groups were all significantly increased compared with control groups (all p < 0.05). CONCLUSIONS Oxidative stress and compensatory activation of antioxidant proteins suggest oxidative stress injury in corneal stromal cells plays an important role in the development of KC in a rabbit model.

['Liu|Ruixing|R|https://orcid.org/0000-0003-0147-0621', 'Yan|Xiaoming|X|']

2021

23640730

Effects of extrusion, infrared and microwave processing on Maillard reaction products and phenolic compounds in soybean.

BACKGROUND The Maillard reaction indicators furosine, hydroxymethylfurfural (HMF), acrylamide and color were determined to evaluate heat effects induced during extrusion, infrared and microwave heating of soybean. In addition, the present paper aimed to study changes in the phenolic compounds, as well as in the overall antioxidant properties of different soybean products in relation to heating at 45-140 °C during the processes. RESULTS Soybean proteins were highly sensible to Maillard reaction and furosine was rapidly formed under slight heating conditions during extrusion and infrared heating. Microwave heating at lower temperatures for a longer time yielded lower acrylamide levels in the final soybean products, as a result of its partial degradation. However, during infrared heating, acrylamide formation greatly increased with decreasing moisture content. After a short time of extrusion and infrared heating at 140 °C and microwave heating at 135 °C for 5 min, concentrations of HMF increased to 11.34, 26.21 and 34.97 µg g(-1), respectively. CONCLUSION The heating conditions caused formation of acrylamide, HMF and furosine in high concentration. The results indicate that the complex structure of soybeans provides protection of phenolic compounds from thermal degradation, and that Maillard reaction products improved the antioxidant properties of heat-treated soybean.

['Zilić|Slađana|S|', 'Mogol|Burçe Ataç|BA|', 'Akıllıoğlu|Gül|G|', 'Serpen|Arda|A|', 'Delić|Nenad|N|', 'Gökmen|Vural|V|']

2014

29366633

Prognostic Value of Pretreatment Albumin-to-Globulin Ratio in Patients With Non-Muscle-Invasive Bladder Cancer.

PURPOSE To investigate the relationship between albumin-to-globulin ratio (AGR) and oncologic outcomes in patients with non-muscle-invasive bladder cancer (NMIBC). PATIENTS AND METHODS We identified 364 patients with primary NMIBC who underwent transurethral surgery between 2000 and 2015. The association between pretreatment AGR and clinicopathologic variables, including oncologic outcomes, was statistically evaluated. RESULTS One hundred twenty patients (33.0%) experienced at least one tumor recurrence, and 23 (6.3%) developed muscle-invasive disease. The median (interquartile range) pretreatment AGR was 1.73 (1.53-1.89). The Kaplan-Meier curve revealed that tumor recurrence was strongly predicted in patients with pretreatment AGR < 1.6, and similar results were observed for disease progression (P < .01 and P < .01, respectively). On multivariate analysis, we found that pretreatment AGR < 1.6 is an independent risk factor for tumor recurrence (hazard ratio, 0.53; P < .01). On univariate analysis, pretreatment AGR < 1.6 was also associated with disease progression (hazard ratio, 0.24; P < .01). CONCLUSION Low pretreatment AGR is an independent risk factor for tumor recurrence and is one risk factor for disease progression in NMIBC patients. This inexpensive and easily accessible biomarker may become useful in selecting patients with NMIBC with higher risk of recurrence and progression.

['Niwa|Naoya|N|', 'Matsumoto|Kazuhiro|K|', 'Ide|Hiroki|H|', 'Nagata|Hirohiko|H|', 'Oya|Mototsugu|M|']

2018

29911089

The use of three-column osteotomy in the treatment of rigid deformities of the adult spine.

Objective To analyze and characterize data about clinical outcome and complication rates in three-column osteotomies (3 CO) for treatment of rigid adult spine deformity (ASD). Methods Baseline and postoperative clinical outcomes, considering the Oswestry Disability Index (ODI) and Scoliosis Research Society-22 (SRS-22) questionnaires, radiographic parameters, and demographic data of patients who underwent 3 CO procedure for fixed ASD treatment were collected. Surgical characteristics and reports of perioperative complications were recorded, as well as those that occurred at a minimum follow-up of 12 months. Results Ten patients underwent 3 CO and had a minimum follow-up of 12 months (mean 24 months). The patients showed significantly improved health-related quality of life scores (ODI, SRS-22 total, function/activity, pain, and appearance). They also presented a significant improvement in all radiographic parameters considered in the study. Taking into account the surgical procedure, the operative time was significantly higher in patients with staged procedure than in patients with single-stage surgery (p = 0.003), with similar estimated blood loss and complication incidence. There were ten complications in six patients (60%), with a mean of 1.0 complication per patient. Conclusions Despite of the high complication rates, 3 CO was an effective technique, considering clinical and radiographic outcomes, to treat complex cases of rigid ASD in a sample of patients operated in a Brazilian spine center, with a minimum follow-up of 12 months.

['Pratali|Raphael de Rezende|RR|', 'Martins|Samuel Machado|SM|', 'Santos|Francisco Prado Eugenio Dos|FPED|', 'Barsotti|Carlos Eduardo Gonçales|CEG|', 'Oliveira|Carlos Eduardo Algaves Soares de|CEAS|']

2018

34212477

Resource availability and heterogeneity shape the self-organisation of regular spatial patterning.

Explaining large-scale ordered patterns and their effects on ecosystem functioning is a fundamental and controversial challenge in ecology. Here, we coupled empirical and theoretical approaches to explore how competition and spatial heterogeneity govern the regularity of colony dispersion in fungus-farming termites. Individuals from different colonies fought fiercely, and inter-nest distances were greater when nests were large and resources scarce-as expected if competition is strong, large colonies require more resources and foraging area scales with resource availability. Building these principles into a model of inter-colony competition showed that highly ordered patterns emerged under high resource availability and low resource heterogeneity. Analysis of this dynamical model provided novel insights into the mechanisms that modulate pattern regularity and the emergent effects of these patterns on system-wide productivity. Our results show how environmental context shapes pattern formation by social-insect ecosystem engineers, which offers one explanation for the marked variability observed across ecosystems.

['Castillo Vardaro|Jessica A|JA|https://orcid.org/0000-0001-9580-6987', 'Bonachela|Juan A|JA|https://orcid.org/0000-0002-3316-8120', 'Baker|Christopher C M|CCM|https://orcid.org/0000-0002-2675-1078', 'Pinsky|Malin L|ML|https://orcid.org/0000-0002-8523-8952', 'Doak|Daniel F|DF|https://orcid.org/0000-0001-8980-3392', 'Pringle|Robert M|RM|https://orcid.org/0000-0001-7487-5393', 'Tarnita|Corina E|CE|https://orcid.org/0000-0003-4476-0012']

2021

33336968

Microbial Contamination of Fresh Produce: What, Where, and How?

Promotion of healthier lifestyles has led to an increase in consumption of fresh produce. Such foodstuffs may expose consumers to increased risk of foodborne disease, as often they are not subjected to processing steps to ensure effective removal or inactivation of pathogenic microorganisms before consumption. Consequently, reports of ready-to-eat fruit and vegetable related disease outbreak occurrences have increased substantially in recent years, and information regarding these events is often not readily available. Identifying the nature and source of microbial contamination of these foodstuffs is critical for developing appropriate mitigation measures to be implemented by food producers. This review aimed to identify the foodstuffs most susceptible to microbial contamination and the microorganisms responsible for disease outbreaks from information available in peer-reviewed scientific publications. A total of 571 outbreaks were identified from 1980 to 2016, accounting for 72,855 infections and 173 deaths. Contaminated leafy green vegetables were responsible for 51.7% of reported outbreaks. Contaminated soft fruits caused 27.8% of infections. Pathogenic strains of Escherichia coli and Salmonella, norovirus, and hepatitis A accounted for the majority of cases. Large outbreaks resulted in particular biases such as the observation that contaminated sprouted plants caused 31.8% of deaths. Where known, contamination mainly occurred via contaminated seeds, water, and contaminated food handlers. There is a critical need for standardized datasets regarding all aspects of disease outbreaks, including how foodstuffs are contaminated with pathogenic microorganisms. Providing food business operators with this knowledge will allow them to implement better strategies to improve safety and quality of fresh produce.

['Machado-Moreira|Bernardino|B|https://orcid.org/0000-0003-2921-710X', 'Richards|Karl|K|https://orcid.org/0000-0002-3703-3450', 'Brennan|Fiona|F|', 'Abram|Florence|F|https://orcid.org/0000-0001-7522-9158', 'Burgess|Catherine M|CM|https://orcid.org/0000-0003-3722-5935']

2019

31845444

Bitter melon fruit extract has a hypoglycemic effect and reduces hepatic lipid accumulation in ob/ob mice.

Bitter melon (Momordica charantia L.) is a vegetable and has been used as traditional medicine. Recently, we reported that bitter melon fruit extracts and its ethyl acetate (EtOAc)-soluble fraction markedly suppressed the expression of proinflammatory genes, including the inducible nitric oxide synthase gene. However, it is unclear whether bitter melon exhibits antidiabetic effects. In this study, we showed that cucurbitacin B, a cucurbitane-type triterpenoid, was present in an EtOAc-soluble fraction and suppressed nitric oxide production in hepatocytes. When the EtOAc-soluble fraction was administered for 7 days to ob/ob mice, a type 2 diabetes mellitus model, the mice fed with this fraction exhibited a significant decrease in body weight and blood glucose concentrations compared with the mice fed without the fraction. The administration of the fraction resulted in significant increases in serum insulin concentrations and the levels of both insulin receptor mRNA and protein in the ob/ob mouse liver. The EtOAc-soluble fraction decreased the interleukin-1β mRNA expression, as well as hepatic lipid accumulation in hepatocytes. Taken together, these results indicate that administration of an EtOAc-soluble fraction improved hyperglycemia and hepatic steatosis, suggesting that this fraction may be responsible for both the antidiabetic and anti-inflammatory effects of bitter melon fruit.

['Dwijayanti|Dinia R|DR|', 'Shimada|Takumi|T|', 'Ishii|Toshinari|T|', 'Okuyama|Tetsuya|T|', 'Ikeya|Yukinobu|Y|', 'Mukai|Eri|E|', 'Nishizawa|Mikio|M|https://orcid.org/0000-0002-1117-2807']

2020

25649283

Cancer of the gingiva.

Cancer of the gingiva is a rare disease in the Western World. It most commonly affects elderly population. Because of its rarity, the reporting on the disease is sparse and often grouped with other subsites of oral cancer, which makes conclusions difficult to interpret. The aim of this paper is to review the literature on gingival cancer as a specific subsite of oral cancer and report on published prognostic factors as well as treatment of local and regional disease. We also present differences between gingival cancer subgroups, mandibular and maxillary gingival cancer. In addition, both surgical and oncological treatments are reviewed. It seems that surgery is the preferred initial treatment approach for the majority of patients with gingival cancer, although adjuvant radiation, with or without chemotherapy, is commonly recommended to increase locoregional control.

['Bark|Rusana|R|', 'Mercke|Claes|C|', 'Munck-Wikland|Eva|E|', 'Wisniewski|Natalie Ann|NA|', 'Hammarstedt-Nordenvall|Lalle|L|']

2016

28260497

Improved Protein Toxin Delivery Based on ATTEMPTS Systems.

BACKGROUND Ribosome-inactivating proteins (RIPs) are wildly found in multiple species of plants, bacteria and fungi. As a special family of protein toxins, RIPs can inhibit protein synthesis and induce cell death via inactivating ribosome in eukaryotic cells. Thus, RIPs have been applied for anti-tumor therapy in the past two decades. However, because of poor cell permeability, nonselective mode of action for tumor cells, poor pharmacokinetic profiles and immunogenicity, their clinical application has been severely constrained. As an effort to overcome these obstacles, tumor-specific monoclonal antibodies (mAb) have been conjugated to RIPs (forming so called "immunotoxins") specifically to increase their cytotoxicity and provide tumor targeting. Nevertheless, immunotoxins yet have not fully resolved all the issues and critical challenges still remain, such as immunogenicity and inability to penetrate into the deep site of tumor. OBJECTIVE To overcome the constrain of immunotoxins, the novel cell-penetrating peptide (CPP)- modified ATTEMPTS systems based on combination of CPP-mediated penetration and antibodymediated tumor targeting, with triggerable drug release function, were developed to achieve effective and safe delivery of protein toxin. RESULTS The CPP-modified ATTEMPTS systems showed effective protamine-triggered CPP-toxin release and thus enhanced CPP-mediated cellular uptake and cytotoxicity. It also showed antibodymediated in vivo tumor targeting and significantly increased in vivo tumor growth suppression with limited systematic toxicity. CONCLUSION The CPP-modified ATTEMPTS systems were developed and demonstrated as a proof-ofconcept for CPP-based protein toxin delivery with triggerable antibody targeting to improve the druggability of protein toxin drugs. The systems showed the potential application of protein toxin clinical translation in anticancer treatment.

['Chen|Yingzhi|Y|', 'Zhang|Meng|M|', 'Min|Kyoung Ah|KA|', 'Wang|Huiyuan|H|', 'Shin|Meong Cheol|MC|', 'Li|Feng|F|', 'Yang|Victor C|VC|', 'Huang|Yongzhuo|Y|']

2018

24776853

Attitudes, motivators, and barriers to a career in surgery: a national study of U.K. undergraduate medical students.

INTRODUCTION Numerous factors influence the career choices of undergraduate medical students, although little work has focused specifically on the surgical specialties. We aimed to investigate medical students' early experiences of surgery along with the motivators and barriers to selecting a surgical career. METHODS All final-year medical students were invited to participate in an online survey. A range of quantitative data (expressed as a proportion of total respondents) and qualitative data (grouped and thematically analyzed) were collected to evaluate students' attitudes to careers in surgery and potential influences on these. RESULTS A total of 482 students from 20 medical schools throughout England and Wales completed the survey. Overall, 91% of respondents had work experience before medical school, in which the greatest satisfaction was reported in the 21% who undertook placements in surgery. In addition, 58% expressed a desire to spend time working abroad and 59% to undertake a period of research; the primary motivator for both was career/professional development. Surgery was the most popular career choice amongst respondents. The 2 most important factors in decisions pertaining to their career were reported as interest in the specialty and work-life balance. Students draw career advice from numerous sources, most commonly mentors (49%) and student surgical societies (46%). Only 1% wanted more website information, with personal contact being paramount. DISCUSSION It is vital for the continuing strength of the profession and the quality of surgery for the public that we continue to attract the best undergraduates into careers in surgery. Surgeons of all grades must be aware that how they are perceived by students affects future career choices. Early experiences of surgery are fundamental in assisting medical students to make informed career choices.

['Sutton|Paul A|PA|', 'Mason|John|J|', 'Vimalachandran|Dale|D|', 'McNally|Scarlett|S|']

2014

33396355

Analytical Parameter Estimation of the SIR Epidemic Model. Applications to the COVID-19 Pandemic.

The SIR (Susceptible-Infected-Removed) model is a simple mathematical model of epidemic outbreaks, yet for decades it evaded the efforts of the mathematical community to derive an explicit solution. The present paper reports novel analytical results and numerical algorithms suitable for parametric estimation of the SIR model. Notably, a series solution of the incidence variable of the model is derived. It is proven that the explicit solution of the model requires the introduction of a new transcendental special function, which is a solution of a non-elementary integral equation. The paper introduces iterative algorithms approximating the incidence variable, which allows for estimation of the model parameters from the numbers of observed cases. The approach is applied to the case study of the ongoing coronavirus disease 2019 (COVID-19) pandemic in five European countries: Belgium, Bulgaria, Germany, Italy and the Netherlands. Incidence and case fatality data obtained from the European Centre for Disease Prevention and Control (ECDC) are analysed and the model parameters are estimated and compared.

['Prodanov|Dimiter|D|']

2020

31105368

A Short-Term Evaluation of a Hospital No Hit Zone Policy to Increase Bystander Intervention in Cases of Parent-to-Child Violence.

This study used a pre/post design to evaluate the implementation of a hospital-wide No Hit Zone (NHZ) bystander intervention around parent-to-child hitting. A total of 2,326 staff completed the pre-NHZ survey and received training about the NHZ policy; 623 staff completed the post-test survey 10 months later. A group of 225 parents participated in the pre-NHZ survey and a second group of 180 participated in the post-NHZ survey, also 10 months later. Compared to staff in the pre-NHZ group, staff in the post-NHZ group had more negative attitudes about spanking and more positive attitudes about intervention when parents hit children in the hospital. Few differences were found among the parent pre- and post-groups. This study demonstrated that NHZs are a feasible way to inform and train hospital staff in ways to intervene during incidents of parent-to-child hitting to promote a safe and healthy medical environment.

['Gershoff|Elizabeth T|ET|', 'Font|Sarah A|SA|', 'Taylor|Catherine A|CA|', 'Garza|Ann Budzak|AB|', 'Olson-Dorff|Denyse|D|', 'Foster|Rebecca H|RH|']

2018

25580360

A rare presentation of metastasis of prostate adenocarcinoma to the stomach and rectum.

Prostate cancer is the second most common cause of cancer death in men in the United States. The most common sites of metastasis include the bone, lymph nodes, lung, liver, pleura, and adrenal glands, whereas metastatic prostate cancer involving the gastrointestinal tract has been rarely reported. A 64-year-old African-American man with a history of prostate cancer presented with anemia. He reported the passing of dark colored stools but denied hematemesis or hematochezia. Colonoscopy revealed circumferential nodularity, and histology demonstrated metastatic carcinoma of the prostate. Esophagogastroduodenoscopy showed hypertrophic folds in the gastric fundus, and microscopic examination revealed tumor cells positive for prostate-specific antigen. Bone scanning and computed tomography of the abdomen and pelvis did not show metastasis. It is crucial to distinguish primary gastrointestinal cancer from metastatic lesions, especially in patients with a history of cancer at another site, for appropriate management.

['Soe|Aye Min|AM|', 'Bordia|Sonal|S|', 'Xiao|Philip Q|PQ|', 'Lopez-Morra|Hernan|H|', 'Tejada|Juan|J|', 'Atluri|Sreedevi|S|', 'Krishnaiah|Mahesh|M|']

2014

32204400

Calcium-Dependent Calpain Activation-Mediated Mitochondrial Dysfunction and Oxidative Stress Are Required for Cytotoxicity of Epinecidin-1 in Human Synovial Sarcoma SW982 Cells.

Synovial sarcoma is a rare but highly malignant and metastatic disease. Despite its relative sensitivity to chemotherapies, the high recurrence and low 5-year survival rate for this disease suggest that new effective therapeutic agents are urgently needed. Marine antimicrobial peptide epinecidin-1 (epi-1), which was identified from orange-spotted grouper (Epinephelus coioides), exhibits multiple biological effects, including bactericidal, immunomodulatory, and anticancer activities. However, the cytotoxic effects and mechanisms of epi-1 on human synovial sarcoma cells are still unclear. In this study, we report that epi-1 exhibits prominent antisynovial sarcoma activity in vitro and in a human SW982 synovial sarcoma xenograft model. Furthermore, we determined that calcium overload-induced calpain activation and subsequent oxidative stress and mitochondrial dysfunction are required for epi-1-mediated cytotoxicity. Interestingly, reactive oxygen species (ROS)-mediated activation of extracellular signal-regulated kinase (ERK) plays a protective role against epi-1-induced cytotoxicity. Our results provide insight into the molecular mechanisms underlying epi-1-induced cell death in human SW982 cells.

['Su|Bor-Chyuan|BC|', 'Li|Chao-Chin|CC|', 'Horng|Jiun-Lin|JL|0000-0002-2121-1910', 'Chen|Jyh-Yih|JY|0000-0001-7858-8236']

2020

24830625

Robot-assisted laparoscopic adrenalectomy: step-by-step technique and comparative outcomes.

BACKGROUND Recent evidence supports the use of robotic surgery for the minimally invasive surgical management of adrenal masses. OBJECTIVE To describe a contemporary step-by-step technique of robotic adrenalectomy (RA), to provide tips and tricks to help ensure a safe and effective implementation of the procedure, and to compare its outcomes with those of laparoscopic adrenalectomy (LA). DESIGN, SETTING, AND PARTICIPANTS We retrospectively reviewed the medical charts of consecutive patients who underwent RA performed by a single surgeon between April 2010 and October 2013. LA cases performed by the same surgeon between January 2004 and May 2010 were considered the control group. SURGICAL PROCEDURE The main steps of our current surgical technique for RA are described in this video tutorial: patient positioning, port placement, and robot docking; exposure of the adrenal gland; identification and control of the adrenal vein; circumferential dissection of the adrenal gland; and specimen retrieval and closure. OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS Demographic parameters and main surgical outcomes were assessed. RESULTS AND LIMITATIONS A total of 76 cases (RA: 30; LA: 46) were included in the analysis. Median tumor size on computed tomography (CT) was significantly larger in the LA group (3cm [interquartile range (IQR): 3] vs 4cm [IQR: 3]; p=0.002). A significantly lower median estimated blood loss was recorded for the robotic group (50ml [IQR: 50] vs 100ml [IQR: 288]; p=0.02). The RA group presented five minor complications (16.7%) and one major (Clavien 3b) complication (3.3%), whereas four minor complications (8.7%) and one major (Clavien 3b) complication (2.3%) were observed in the LA group. No significant difference was noted between groups in terms of malignant histology (p=0.66) and positive margin rate (p=0.60). Distribution of pheochromocytomas in the LA group was significantly higher than in the RA group (43.5% vs 16.7%; p=0.02). CONCLUSIONS The standardization of each surgical step optimizes the RA procedure. The robotic approach can be applied for a wide range of adrenal indications, recapitulating the safety and effectiveness of open surgery and potentially improving the outcomes of standard laparoscopy. PATIENT SUMMARY In this report we detail our surgical technique for robotic removal of adrenal masses. This procedure has been standardized and can be offered to patients, with excellent outcomes.

['Brandao|Luis Felipe|LF|', 'Autorino|Riccardo|R|', 'Zargar|Homayoun|H|', 'Krishnan|Jayram|J|', 'Laydner|Humberto|H|', 'Akca|Oktay|O|', 'Mir|Maria Carmen|MC|', 'Samarasekera|Dinesh|D|', 'Stein|Robert|R|', 'Kaouk|Jihad|J|']

2014

29250322

Rehabilitation following hemorrhagic stroke: building the case for stroke-subtype specific recovery therapies.

Intracerebral hemorrhage (ICH), a form of brain bleeding and minor subtype of stroke, leads to significant mortality and long-term disability. There are currently no validated approaches to promote functional recovery after ICH. Research in stroke recovery and rehabilitation has largely focused on ischemic stroke, but given the stark differences in the pathophysiology between ischemic and hemorrhagic stroke, it is possible that strategies to rehabilitate the brain in distinct stroke subtypes will be different. Here, we review our current understanding of recovery after primary intracerebral hemorrhage with the intent to provide a framework to promote novel, stroke-subtype specific approaches.

['Kitago|Tomoko|T|', 'Ratan|Rajiv R|RR|']

2017

26989941

Beneficial role of bacterial endophytes in heavy metal phytoremediation.

Phytoremediation is an emerging technology that uses plants and their associated microbes to clean up pollutants from the soil, water and air. In recent years, phytoremediation assisted by bacterial endophytes has been highly recommended for cleaning up of metal polluted soils since endophytic bacteria can alleviate metal toxicity in plant through their own metal resistance system and facilitate plant growth under metal stress. Endophytic bacteria improve plant growth in metal polluted soils in two different ways: 1) directly by producing plant growth beneficial substances including solubilization/transformation of mineral nutrients (phosphate, nitrogen and potassium), production of phytohormones, siderophores and specific enzymes; and 2) indirectly through controlling plant pathogens or by inducing a systemic resistance of plants against pathogens. Besides, they also alter metal accumulation capacity in plants by excreting metal immobilizing extracellular polymeric substances, as well as metal mobilizing organic acids and biosurfactants. The present work aims to review the progress of recent research on the isolation, identification and diversity of metal resistant endophytic bacteria and illustrate various mechanisms responsible for plant growth promotion and heavy metal detoxification/phytoaccumulation/translocation in plants.

['Ma|Ying|Y|', 'Rajkumar|Mani|M|', 'Zhang|Chang|C|', 'Freitas|Helena|H|']

2016

31141727

Abnormal functional network of the thalamic subregions in adult patients with obsessive-compulsive disorder.

The thalamus plays an important role in pathological mechanisms underlying obsessive-compulsive disorder (OCD). As the thalamus is a heterogeneous brain region, functional connectivity (FC) between thalamic subregions and other brain regions is worth investigating in OCD. In addition, the relationship between abnormal FC and clinical symptoms is still unclear. In this study, we used resting-state functional magnetic resonance imaging to scan 45 OCD patients and 43 well-matched healthy controls (HCs). Thalamic subregions were defined according to the Human Brainnetome Atlas. The fractional amplitude of low-frequency fluctuations (fALFF) and FC seeding-based connectivity were compared using a two-sample t-test. Correlations between abnormal FC and clinical symptoms were analyzed in OCD patients. Compared with HCs, increased fALFF was found in the bilateral thalamus, and increased FC was observed between the right posterior parietal thalamus (PPtha) and left middle occipital gyrus (LMOG) and between the right occipital thalamus (Otha) and right middle occipital gyrus (RMOG) in OCD patients. In addition, OCD patients had reduced FC between the left sensory thalamus (Stha) and left orbital inferior frontal gyrus, right PPtha and left prefrontal cortex, and between the right Otha and left inferior parietal gyrus (LIPG), respectively. Within the OCD group, the FC between right PPtha-LMOG was correlated with severity of clinical symptoms. These results revealed that the FC between the thalamus and occipital lobe is related to obsessive-compulsive symptoms in OCD patients. This finding provides more accurate information about the involvement of the thalamus in the pathophysiology of OCD.

['Li|Kun|K|', 'Zhang|Haisan|H|', 'Yang|Yongfeng|Y|', 'Zhu|Jianli|J|', 'Wang|Bi|B|', 'Shi|Yanli|Y|', 'Li|Xianrui|X|', 'Meng|Zhang|Z|', 'Lv|Luxian|L|', 'Zhang|Hongxing|H|']

2019

27563359

From Spin to Swindle: Identifying Falsification in Financial Text.

Despite legislative attempts to curtail financial statement fraud, it continues unabated. This study makes a renewed attempt to aid in detecting this misconduct using linguistic analysis with data mining on narrative sections of annual reports/10-K form. Different from the features used in similar research, this paper extracts three distinct sets of features from a newly constructed corpus of narratives (408 annual reports/10-K, 6.5 million words) from fraud and non-fraud firms. Separately each of these three sets of features is put through a suite of classification algorithms, to determine classifier performance in this binary fraud/non-fraud discrimination task. From the results produced, there is a clear indication that the language deployed by management engaged in wilful falsification of firm performance is discernibly different from truth-tellers. For the first time, this new interdisciplinary research extracts features for readability at a much deeper level, attempts to draw out collocations using n-grams and measures tone using appropriate financial dictionaries. This linguistic analysis with machine learning-driven data mining approach to fraud detection could be used by auditors in assessing financial reporting of firms and early detection of possible misdemeanours.

['Minhas|Saliha|S|', 'Hussain|Amir|A|']

2016

32260502

Wood Density and Moisture Content Estimation by Drilling Chips Extraction Technique.

The novelty of this study is the development of an accurate wood moisture content (MC) estimation method based on a relatively brand-new, non-destructive testing technique (drilling chips extraction). The method is especially important in the assessment of existing timber structures, where non-destructive testing (NDT) results are affected by wood MC and should be adjusted to a reference MC, usually 12%. In the assessment of timber structures, it is not possible to determine MC by oven drying method and this should be estimated. Electrical resistance and capacitance are the conventional methods used for MC estimation. This research work aims to present an accurate MC estimation method based on the drilling chips extraction technique. For that, 99 specimens (90 × 65 × 38 mm3) from three softwood and hardwood species covering a wide range of densities (from 355 to 978 kg m-3) were tested after conditioning at five different MCs (5%, 10%, 15%, 20%, 25%). The Wood Extractor device based on the drilling chips extraction technique was used. The mass of the chips collected (drilling residue) from each drill was recorded. The results show that the MC of the chips extracted was statistically significantly different than the MC of the specimen and cannot be directly used as MC determination. However, the chips MC can be used as an estimator of specimen MC with high determination coefficients (R2 from 71% to 86%). As the main result, models to estimate density directly adjusted to a reference 12% MC from the wet and dry mass of chips extracted were developed with an R2 of 98%. In sum, the drilling chips extractor is a dependable and straightforward method to estimate MC and density from only one measurement. Density adjusted to a reference 12% MC can be directly estimated from a single model.

['Martínez|Roberto D|RD|0000-0002-0567-1781', 'Balmori|José-Antonio|JA|0000-0003-4385-5765', 'F Llana|Daniel|D|0000-0001-7758-9456', 'Bobadilla|Ignacio|I|0000-0002-4627-3130']

2020

32667062

Nanotechnology improves delivery efficiency and bioavailability of tea polyphenols.

Tea polyphenols (TPP) have shown various biological activities. However, due to their poor stability in the gastrointestinal (GI) tract, TPP exhibit low absorption and bioavailability which limit their applications in food fields. Recently, several studies have focused on the utilization of nanotechnology to solve these problems. In this review, we introduced the embedding materials and methods of TPP-loaded nanoparticles and the potential mechanisms for improving bioavailability, such as to protect TPP from pH stress, enzymes and ions of the GI tract, and increase of the permeability. Furthermore, future challenges and application prospects of nanoparticles as carriers for the delivery of TPP were also discussed. PRACTICAL APPLICATIONS: Nanotechnology is currently an emerging field in food science, which can be employed to increase the systemic delivery and bioavailability of phytochemicals. Due to the improved bioavailability, TPP-loaded nanoparticles can be developed as potential functional food. A better understanding of the nano-embedding technology and the potential mechanisms will allow us to better utilize nanomaterials to increase the bioavailability of TPP and expand their applications.

['Yin|Chunyan|C|', 'Cheng|Lu|L|', 'Zhang|Xin|X|0000-0002-5795-9197', 'Wu|Zufang|Z|0000-0001-8136-0312']

2020

33467961

Ocular Trauma During the COVID-19 Lockdown.

: To identify the impact the COVID-19 lockdown had on the presentation and management of sight-threatening ocular trauma.: A retrospective cohort analysis of all patients who presented to the Ophthalmology department of Royal Hallamshire Hospital Sheffield with serious ocular trauma during the COVID-19 lockdown period was performed. Data on mechanism of injury and date of injury, presentation, and surgical repair were collected. This process was repeated for the same dates in the previous 5 years for comparison.: During the COVID-19 lockdown period, we saw 10 cases of serious ocular trauma (4 globe ruptures, 4 full-thickness lid lacerations, and 2 intra-ocular foreign bodies). This is 3.33 times the average number of cases over the previous 5 years. The delay between injury and presentation rose to 1.1 days compared to 0.33 days pre-COVID; however, the time taken between presentation to surgery was only slightly affected (12 hours in 2020 compared to 11.38 hours pre-COVID).: During the COVID-19 lockdown, the number of serious ocular trauma cases was more than three times the average of the previous 5 years. This increase is partially due to more DIY injuries as people stayed at home, but also surprisingly an increase in falls. There did appear to be a longer delay between injury and presentation, suggesting that patients were reluctant to come into hospital during the pandemic. Surgery was performed within 12 hours on average for both groups, reassuringly indicating that sight-saving surgery was not delayed despite extraordinary circumstances.

['Stedman|Emily N|EN|', 'Jefferis|Joanna M|JM|', 'Tan|Jennifer H|JH|']

2021

32580140

Nanoscale regulation of L-type calcium channels differentiates between ischemic and dilated cardiomyopathies.

BACKGROUND Subcellular localization and function of L-type calcium channels (LTCCs) play an important role in regulating contraction of cardiomyocytes. Understanding how this is affected by the disruption of transverse tubules during heart failure could lead to new insights into the disease. METHODS Cardiomyocytes were isolated from healthy donor hearts, as well as from patients with cardiomyopathies and with left ventricular assist devices. Scanning ion conductance and confocal microscopy was used to study membrane structures in the cells. Super-resolution scanning patch-clamp was used to examine LTCC function in different microdomains. Computational modeling predicted the impact of these changes to arrhythmogenesis at the whole-heart level. FINDINGS We showed that loss of structural organization in failing myocytes leads to re-distribution of functional LTCCs from the T-tubules to the sarcolemma. In ischemic cardiomyopathy, the increased LTCC open probability in the T-tubules depends on the phosphorylation by protein kinase A, whereas in dilated cardiomyopathy, the increased LTCC opening probability in the sarcolemma results from enhanced phosphorylation by calcium-calmodulin kinase II. LVAD implantation corrected LTCCs pathophysiological activity, although it did not improve their distribution. Using computational modeling in a 3D anatomically-realistic human ventricular model, we showed how LTCC location and activity can trigger heart rhythm disorders of different severity. INTERPRETATION Our findings demonstrate that LTCC redistribution and function differentiate between disease aetiologies. The subcellular changes observed in specific microdomains could be the consequence of the action of distinct protein kinases. FUNDING This work was supported by NIH grant (ROI-HL 126802 to NT-JG) and British Heart Foundation (grant RG/17/13/33173 to JG, project grant PG/16/17/32069 to RAC). Funders had no role in study design, data collection, data analysis, interpretation, writing of the report.

['Sanchez-Alonso|Jose L|JL|', 'Loucks|Alexandra|A|', 'Schobesberger|Sophie|S|', 'van Cromvoirt|Ankie M|AM|', 'Poulet|Claire|C|', 'Chowdhury|Rasheda A|RA|', 'Trayanova|Natalia|N|', 'Gorelik|Julia|J|']

2020

26197262

Safety of first-trimester uterine evacuation in the outpatient setting for women with common chronic conditions.

OBJECTIVE We compared complications of outpatient first-trimester uterine evacuation between women with medical comorbidities and healthy peers. STUDY DESIGN We examined the medical histories and procedure outcomes of women receiving first-trimester uterine evacuations between 01/02/2009 and 03/07/2014. We compared women without medical problems to those reporting diabetes, hypertension, obesity (body mass index ≥30.0 or weight ≥200 lbs), HIV, epilepsy, asthma, thyroid disease and/or bleeding/clotting disorders. We compared incidence of any of the following: resuction, uterine perforation, estimated blood loss >100 cc and cervical laceration. RESULTS A total of 1960 women met inclusion criteria; 597 (30%) had ≥1 comorbidity. When compared to women without medical morbidities, women with common chronic conditions were older (28.3±6.7 vs. 27.3±6.7 years, p<.01), less likely to be primigravid (29.1% vs. 35.7%, p<.01) and more likely to have had a prior cesarean delivery (24.9% vs. 15.7%, p<.001). Gestational age and indication for evacuation were similar for the groups. The overall complication rate was 2.9%. There was no difference in complications between the group of patients with at least one comorbidity compared to the group of women without any comorbidity (OR=0.9, 95% CI 0.5, 1.6). Additionally, there were no specific medical comorbidities that led to an increased complication rate. The only significant predictor of complication was history of cesarean delivery (OR=1.9, 95% CI 1.1, 3.4). CONCLUSION Women with common chronic conditions undergoing outpatient first-trimester uterine evacuation do not appear to be at greater risk of complications compared to healthy peers. While a careful medical history is always required, providers may feel reassured that complications remain infrequent. IMPLICATIONS Women with common chronic conditions undergoing outpatient first-trimester uterine evacuation do not appear to be at greater risk of complications compared to healthy peers.

['Guiahi|Maryam|M|', 'Schiller|Georgia|G|', 'Sheeder|Jeanelle|J|', 'Teal|Stephanie|S|']

2015

27460156

Spatial Clustering of Escherichia coli with Reduced Susceptibility to Cefotaxime and Ciprofloxacin among Dairy Cattle Farms Relative to European Starling Night Roosts.

European starlings (Sturnus vulgaris) have been implicated in the dispersal of zoonotic enteric pathogens. However, their role in disseminating antimicrobial-resistant organisms through their home range has not been clearly established. The aim of this study was to determine whether starling night roosts served as foci for spreading organisms with reduced susceptibility to antimicrobials among dairy cattle farms. Bovine faecal pats were collected from 150 dairy farms in Ohio. Each farm was visited twice (in summer and fall) between 2007 and 2009. A total of 1490 samples (10 samples/farm over two visits) were tested for Escherichia coli with reduced susceptibility to cefotaxime and ciprofloxacin. Using a spatial scan statistic, focal scans were conducted to determine whether clusters of farms with a high prevalence of organisms with reduced susceptibility to cefotaxime and ciprofloxacin surrounded starling night roosts. Faecal pats 13.42% and 13.56% of samples carried Escherichia coli with reduced susceptibility to cefotaxime and ciprofloxacin, respectively. Statistically significant (P < 0.05) spatial clusters of faecal pats with high prevalence of Escherichia coli showing reduced susceptibility to cefotaxime and ciprofloxacin were identified around these night roosts. This finding suggests that the risk of carriage of organisms with reduced susceptibility to antimicrobials in cattle closer to starling night roosts was higher compared to cattle located on farms further from these sites. Starlings might have an important role in spreading antimicrobial-resistant E. coli to livestock environments, thus posing a threat to animal and public health.

['Medhanie|G A|GA|', 'Pearl|D L|DL|', 'McEwen|S A|SA|', 'Guerin|M T|MT|', 'Jardine|C M|CM|', 'Schrock|J|J|', 'LeJeune|J T|JT|']

2017

30529101

Association between adiponectin T94G polymorphism and resistant hypertension in young-onset Taiwanese patients.

Young-onset hypertensives (YOHs) with resistant hypertension (RH) have a greater long-term cardiovascular risk. The present study examined whether a functional adiponectin T94G polymorphism (rs2241766) is associated with RH in YOHs. We analyzed data from the Academia Sinica Collaborative Study on Hypertension Genetics in Taiwanese subjects to compare rs2241766 between YOHs with and without RH (≤50 years of age). RH was defined as the need for at least 3 drugs, including a diuretic to control blood pressure. Genotyping of rs2241766 was performed using TaqMan allelic discrimination. A total of 861 YOHs were enrolled and 54 had RH in enrolled population. For the rs2241766 in the allelic model, the odds ratio (OR) of RH allele frequency was 2.45 (p = 0.008), and there was a linear relationship between allele numbers and the presence of RH (p = 0.005). In multivariate analysis, the rs2241766 (OR = 2.766, p = 0.002), age (OR = 1.103, p = 0.001), uric acid (OR = 1.322, p = 0.001), high-sensitivity C-reactive protein (OR = 2.769, p = 0.001) and aldosterone (OR = 1.004, p = 0.001) were independently associated with the presence of RH. In the Taiwanese population, the adiponectin T94G polymorphism (rs2241766) is associated with RH in YOHs.

['Jhuo|Shih-Jie|SJ|', 'Tsai|Wei-Chung|WC|', 'Lee|Hsiang-Chun|HC|', 'Lin|Tsung-Hsien|TH|', 'Lee|Kun-Tai|KT|', 'Lai|Wen-Ter|WT|']

2019

33290142

The Efficacy of the Novel Bioavailable Curcumin (Cureit) in the Management of Sarcopenia in Healthy Elderly Subjects: A Randomized, Placebo-Controlled, Double-Blind Clinical Study.

Sarcopenia represents the progressive loss of skeletal muscles, which occurs as a result of aging. Plant-derived phytochemicals have the potential ability to manage sarcopenial conditions. The randomized, placebo-controlled, double-blind clinical study involving thirty subjects evaluated the efficacy of Cureit™ supplementation in the management of sarcopenial conditions by measuring the variables, such as hand grip strength, weight lift strength, time/distance before feeling tired after cycling, walking and climbing stairs, and Karnofsky performance scale index along with effects on general fitness, such as protein, urea, oxidative stress, and hematology parameters. The results showed that Cureit supplementation resulted in a significant increase of 1.43% (P < .001) in the handgrip strength compared with placebo. The weight-lifting capacity of subjects supplemented with Cureit showed an increase of 6.08%, whereas placebo showed a 4.54% decrease after the end of the study period. The results demonstrated that the Cureit tended to have a positive impact on distance covered before feeling tired as shown by an increase (P = .09) of 5.51%, compared with placebo group, which showed an increase of 2.29%. The time taken to walk the same distance was reduced in the Cureit group (1.15%), whereas in the placebo group, it was increased (2.02%). Cureit plays a significant role in the management of sarcopenia by anti-inflammatory action, increased hand grip strength, antifatigue effects, and muscle protein management. Clinical Trials Registry-India registration no. CTRI/2018/05/014176.

['Varma|Karthik|K|', 'Amalraj|Augustine|A|', 'Divya|Chandradhara|C|', 'Gopi|Sreeraj|S|']

2021

28427115

Personality assessment and model comparison with behavioral data: A statistical framework and empirical demonstration with bonobos (Pan paniscus).

Interest in quantifying consistent among-individual variation in primate behavior, also known as personality, has grown rapidly in recent decades. Although behavioral coding is the most frequently utilized method for assessing primate personality, limitations in current statistical practice prevent researchers' from utilizing the full potential of their coding datasets. These limitations include the use of extensive data aggregation, not modeling biologically relevant sources of individual variance during repeatability estimation, not partitioning between-individual (co)variance prior to modeling personality structure, the misuse of principal component analysis, and an over-reliance upon exploratory statistical techniques to compare personality models across populations, species, and data collection methods. In this paper, we propose a statistical framework for primate personality research designed to address these limitations. Our framework synthesizes recently developed mixed-effects modeling approaches for quantifying behavioral variation with an information-theoretic model selection paradigm for confirmatory personality research. After detailing a multi-step analytic procedure for personality assessment and model comparison, we employ this framework to evaluate seven models of personality structure in zoo-housed bonobos (Pan paniscus). We find that differences between sexes, ages, zoos, time of observation, and social group composition contributed to significant behavioral variance. Independently of these factors, however, personality nonetheless accounted for a moderate to high proportion of variance in average behavior across observational periods. A personality structure derived from past rating research receives the strongest support relative to our model set. This model suggests that personality variation across the measured behavioral traits is best described by two correlated but distinct dimensions reflecting individual differences in affiliation and sociability (Agreeableness) as well as activity level, social play, and neophilia toward non-threatening stimuli (Openness). These results underscore the utility of our framework for quantifying personality in primates and facilitating greater integration between the behavioral ecological and comparative psychological approaches to personality research.

['Martin|Jordan S|JS|0000-0001-8704-6076', 'Suarez|Scott A|SA|']

2017

30423272

Utility of Droplet Digital PCR Assay for Quantitative Detection of Norovirus in Shellfish, from Production to Consumption in Guangxi, China.

OBJECTIVE Shellfish are recognized as important vehicles of norovirus-associated gastroenteritis. The present study aimed to monitor norovirus contamination in oysters along the farm-to-fork continuum in Guangxi, a major oyster production area in Southwestern China. METHODS Oyster samples were collected monthly from farms, markets, and restaurants, from January to December 2016. Norovirus was detected and quantified by one-step reverse transcription-droplet digital polymerase chain reaction (RT-ddPCR). RESULTS A total of 480 oyster samples were collected and tested for norovirus genogroups I and II. Norovirus was detected in 20.7% of samples, with genogroup II predominating. No significant difference was observed in norovirus prevalence among different sampling sites. The norovirus levels varied widely, with a geometric mean of 19,300 copies/g in digestive glands. Both norovirus prevalence and viral loads showed obvious seasonality, with a strong winter bias. CONCLUSION This study provides a systematic analysis of norovirus contamination 'from the farm to the fork' in Guangxi. RT-ddPCR can be a useful tool for detection and quantification of low amounts of norovirus in the presence of inhibitors found particularly in foodstuffs. This approach will contribute to the development of strategies for controlling and reducing the risk of human illness resulting from shellfish consumption.

['Tan|Dong Mei|DM|', 'Lyu|Su Ling|SL|', 'Liu|Wei|W|', 'Zeng|Xian Ying|XY|', 'Lan|Lan|L|', 'Qu|Cong|C|', 'Zhuge|Shi Yang|SY|', 'Zhong|Yan Xu|YX|', 'Xie|Yi Hong|YH|', 'Li|Xiu Gui|XG|']

2018

28342006

Potential synergistic effects of a mixture of mineral trioxide aggregate (MTA) cement and Bacillus subtilis in dental caries treatment.

Bacillus subtilis is nonpathogenic in humans and produces a number of useful substances and, therefore, this bacterium is used in probiotic therapy. There have been trials of B. subtilis for patients with periodontitis, but not for patients with caries. Similarly, mineral trioxide aggregate (MTA) cement has been widely used for endodontic treatment, but there are few reports of its use for caries. Therefore, examinations were performed regarding the benefits of addition of B. subtilis to MTA cement for treatment of dental caries. Indirect pulp capping with a mixture of MTA cement and B. subtilis spore powder is effective for avoiding pulpectomy or tooth extraction in such cases (personal communication). This study was planned to examine the scientific basis of this clinical finding, with examination of possible synergistic effects of MTA cement and B. subtilis. From these experiments, the following five results were obtained: (1) B. subtilis did not proliferate in liquid-culture media at pH ≥10. (2) B. subtilis proliferated when mixed with MTA cement. (3) There was no significant difference in proliferation of B. subtilis under aerobic and microaerobic conditions. (4) B. subtilis exhibited antibacterial effects on Staphylococcus aureus and Lactobacillus casei. (5) MTA cement exhibited antibacterial effects on S. aureus and Streptococcus mutans, but not on B. subtilis. These results support the hypothesis that a combination of B subtilis and MTA cement is likely to be clinically useful for treatment of dental caries.

['Oka|Shunya|S|']

2018

30414100

Incidence of initial spinal metastasis in glioblastoma patients and the importance of spinal screening using MRI.

PURPOSE Intracranial glioblastomas with simultaneous spinal lesions prior to chemoradiation therapy or craniotomy, defined as initial spinal metastasis, are not well understood. Herein, we investigated intracranial glioblastoma and demonstrated the importance of spinal screening using gadolinium enhanced spinal magnetic resonance imaging (Gd-MRI). METHODS Consecutive adult patients with intracranial glioblastoma were treated between 2010 and 2014 and received spinal screening using Gd-MRI. Spinal screening was performed regardless of spine-related symptoms, and patients presenting with and without initial spinal metastasis (spinal and non-spinal groups, respectively) were compared based on patient demographics, tumor characteristics, radiological and molecular features, and overall survival (OS). RESULTS During the study period, 116 glioblastoma cases were treated and 87 of these (76%) underwent spinal screening. Among these patients, 11 (13%) were included in the spinal group, and 76 (87%) were included in the non-spinal group. All patients of the spinal group were free of symptoms related to spinal lesions. Compared with the non-spinal group, intracranial lesions of the spinal group presented higher incidences of intracranial dissemination and were located at subventricular zones (P = 0.0012 and 0.020, respectively). MIB-1 labeling index, molecular alterations such as IDH1 mutation, TERT promoter mutation, and immunoreactivity of ATRX and MGMT did not differ between two groups. OS was significantly shorter in the spinal group than in the non-spinal group (P = 0.0054). CONCLUSIONS This study revealed a relatively high incidence of spinal metastasis. A subset of glioblastoma patients benefited from spinal screening, through which early detection of asymptomatic spinal metastasis was achieved.

['Shibahara|Ichiyo|I|', 'Saito|Ryuta|R|http://orcid.org/0000-0002-2484-1360', 'Osada|Yoshinari|Y|', 'Kanamori|Masayuki|M|', 'Sonoda|Yukihiko|Y|', 'Kumabe|Toshihiro|T|', 'Mugikura|Shunji|S|', 'Watanabe|Mika|M|', 'Tominaga|Teiji|T|']

2019

32382796

Spatial Distributions, Sources and Risks of Polycyclic Aromatic Hydrocarbons in Sediments from Ziya River System, Northern China.

With the development of urbanization and industrialization, Ziya River Plain (ZYRP) had become one of the most polluted regions of polycyclic aromatic hydrocarbons (PAHs) in north China. The distribution of PAHs in sediments were investigated, and then their sources and risks were evaluated. The results showed that the total PAHs varied from 3372 to 92,948 μg/kg, and heavy pollution was found in the upstream. Both the isomer pair ratios of PAH and principal components analysis (PCA) revealed that fossil fuel combustion was the mainly contributes (69.1%), followed by wood and coal combustion (26.7%). The ecological risk for the whole area was moderate, with the highest risk in Niuwei River (NWR). and significant correlation was found between the ecological risk and high-molecular-weight (HMW) of PAHs (r2 > 0.99, p < 0.01). Our findings would give insights into the mitigation of sedimentary PAHs pollution in north China.

['Wang|Zhipeng|Z|', 'Fan|Aoxiang|A|', 'Tang|Wenzhong|W|', 'Zhang|Hong|H|http://orcid.org/0000-0002-7650-0998', 'Zhang|Shuzhen|S|']

2021

29438878

Crushing hope: Short term responses to tragedy vary by hopefulness.

This paper explores the consequences of hopefulness when the environment changes. Much literature has documented the importance of a positive outlook in pursuing investments in health and education that pay off in the future. A question that has received less attention is whether a positive outlook creates resilience in the face of setbacks or whether a positive outlook may be a disadvantage in extreme circumstances, especially when there is a large mismatch between expectations and reality. This paper uses the coincidental interview schedule of the Add Health data (N = 15,024) around the terrorist attack of September 11, 2001 to examine interactions with this environmental shock and previously elicited measures of hopefulness. The results suggest that increases in depressive symptoms following the attack are concentrated among those young adults who initially expressed the most hopefulness in the future as teenagers.

['Fletcher|Jason|J|']

2018

25890828

Detecting unforgiving roadside contributors through the severity analysis of ran-off-road crashes.

The objective of this paper is to study the contributors influencing ran-off-road (ROR) crash severities in a setting that has not been analysed in the literature, namely on freeways not designed according to the "forgiving roadside" concept. To accomplish the analysis, ROR crash data were collected on freeway road sections in Portugal and multinomial and mixed logit models were estimated using the driver injury and the most severely injured occupant as outcome variables. Our results are in line with previous findings reported in the literature on ROR crash severity in a number of distinct settings. Most importantly, this study shows the contribution of critical slopes and vehicle rollover towards fatal injuries and highlights the importance of introducing the "forgiving roadside" concept to mitigate ROR crash severity in Portuguese freeways. The study also indicates the importance of protecting errant vehicles particularly in horizontal curves, as these are linked with fatalities. Finally, the empirical findings from the developed models revealed problems in current Portuguese roadside design, especially with regards to criteria for forgiving slopes provision and warrants for safety barrier installation.

['Roque|Carlos|C|', 'Moura|Filipe|F|', 'Lourenço Cardoso|João|J|']

2015

28612106

The kynurenine:tryptophan ratio as a predictor of incident type 2 diabetes mellitus in individuals with coronary artery disease.

AIMS/HYPOTHESIS The tryptophan metabolite kynurenine has potent immune modulatory and vasoactive properties. Experimental data implicate kynurenine in obesity-related morbidities. Epidemiological studies are, however, sparse. We evaluated associations of the plasma and urine kynurenine:tryptophan ratio (KTR) to incident type 2 diabetes. METHODS We followed 2519 individuals with coronary artery disease (CAD; 73.1% men) without diabetes at baseline for a median of 7.6 years, during which 173 (6.9%) new incidences of type 2 diabetes were identified. Multivariate Cox regression analyses were applied to investigate the prospective relationships of plasma and urine KTR with new onset type 2 diabetes. RESULTS At inclusion, mean (SD) age was 61.3 (10.4) years, BMI was 25.9 (3.71) kg/m2 and median (interquartile range) HbA1c was 5.6% (5.0%-6.0%) (38 [31-42] mmol/mol). Plasma KTR was not significantly related to type 2 diabetes risk. By contrast, urine KTR showed a strong positive association. Comparing quartile 4 with quartile 1, the HRs (95% CIs) were 2.59 (1.56, 4.30) and 2.35 (1.39, 3.96) in the age- and sex-adjusted and multivariate models, respectively. CONCLUSIONS/INTERPRETATION Urine KTR is a strong predictor of incident type 2 diabetes in individuals with CAD. Potential clinical implications and possible pathogenic roles of renal kynurenine excretion in type 2 diabetes development should be further elucidated.

['Rebnord|Eirik W|EW|', 'Strand|Elin|E|', 'Midttun|Øivind|Ø|', 'Svingen|Gard F T|GFT|', 'Christensen|Monika H E|MHE|', 'Ueland|Per M|PM|', 'Mellgren|Gunnar|G|', 'Njølstad|Pål R|PR|', 'Tell|Grethe S|GS|', 'Nygård|Ottar K|OK|', 'Pedersen|Eva R|ER|']

2017

31308197

Prospective study on embolization of intracranial aneurysms with the pipeline device: the PREMIER study 1 year results.

BACKGROUND Preliminary clinical studies on the safety and efficacy of the pipeline embolization device (PED) for the treatment of small/medium aneurysms have demonstrated high occlusion rates with low complications. OBJECTIVE To evaluate the safety and effectiveness of the PED for treatment of wide necked small and medium intracranial aneurysms. METHODS PREMIER is a prospective, multicenter, single arm trial. Patients were treated with the PED for unruptured wide necked aneurysms, measuring ≤12 mm along the internal carotid artery or vertebral artery, between July 2014 and November 2015. At 1 year post-procedure, the primary effectiveness endpoint was complete occlusion (Raymond grade 1) without major parent vessel stenosis (≤50%) or retreatment, and the primary safety endpoint was major stroke in the territory supplied by the treated artery or neurologic death. RESULTS A total of 141 patients were treated with PEDs (mean age 54.6±11.3 years, 87.9% (124/141) women). Mean aneurysm size was 5.0±1.92 mm, and 84.4% (119/141) measured <7 mm. PED placement was successful in 99.3% (140/141) of patients. Mean number of PEDs implanted per patient was 1.1±0.26; a single PED was used in 92.9% (131/141) of patients. At 1 year, 97.9% (138/141) of patients underwent follow-up angiography with 76.8% (106/138) of patients having met the study's primary effectiveness endpoint. The combined major morbidity and mortality rate was 2.1% (3/140). CONCLUSIONS Treatment of wide necked small/medium aneurysms with the PED results in high rates of complete occlusion without significant parent vessel stenosis and low rates of permanent neurologic complications. TRIAL REGISTRATION NCT02186561.

['Hanel|Ricardo A|RA|', 'Kallmes|David F|DF|', 'Lopes|Demetrius Klee|DK|', 'Nelson|Peter Kim|PK|', 'Siddiqui|Adnan|A|', 'Jabbour|Pascal|P|', 'Pereira|Vitor M|VM|', 'Szikora István|Istvan|I|', 'Zaidat|Osama O|OO|http://orcid.org/0000-0003-4881-4698', 'Bettegowda|Chetan|C|', 'Colby|Geoffrey P|GP|http://orcid.org/0000-0002-3376-0933', 'Mokin|Maxim|M|http://orcid.org/0000-0003-4270-8667', 'Schirmer|Clemens|C|', 'Hellinger|Frank R|FR|', 'Given Ii|Curtis|C|', 'Krings|Timo|T|', 'Taussky|Philipp|P|http://orcid.org/0000-0001-9247-0146', 'Toth|Gabor|G|http://orcid.org/0000-0002-3646-3635', 'Fraser|Justin F|JF|http://orcid.org/0000-0002-5980-3989', 'Chen|Michael|M|', 'Priest|Ryan|R|', 'Kan|Peter|P|', 'Fiorella|David|D|http://orcid.org/0000-0002-2677-8780', 'Frei|Don|D|', 'Aagaard-Kienitz|Beverly|B|', 'Diaz|Orlando|O|', 'Malek|Adel M|AM|', 'Cawley|C Michael|CM|', 'Puri|Ajit S|AS|']

2020

31879231

Midterm outcomes in postpartum women following endovenous treatment for acute iliofemoral deep vein thrombosis.

BACKGROUND The development of post-thrombotic syndrome (PTS) after iliofemoral deep vein thrombosis (DVT) continues to be a considerable issue for both pregnant and postpartum women with rates as high as 70% among those managed with anticoagulation alone. This study aims to characterize the outcomes of interventional treatment for acute iliofemoral DVT in this at-risk population. METHODS A retrospective analysis of all postpartum patients treated for acute iliofemoral DVT with lysis and stenting between January 2012 and December 2017 at a referral center. Patient demographics, risk factors, procedural factors. and complications were collected. Post-treatment outcomes were compared with all nonpostpartum females treated within the same time period. These included the severity of PTS evaluated using the Villalta scale, duration of vessel patency and factors affecting reintervention timing and success. Further detailed review of cases needing reintervention was also conducted through a retrospective review of documentation and an analysis of all imaging by a consultant radiologist. RESULTS A total of 11 postpartum women were identified. The median age was 28 years (range, 22-41 years) and intervention was performed at a median of 3 weeks after birth (range 2-12 weeks). No major or minor complications associated with intervention were reported in any patients. The median Villalta score was 3 at 6 months, improving to 2 at 12 months. Overall, two patients were classified as mild having PTS (18%), with no cases of moderate to severe PTS. On comparison with nonpostpartum (n = 68) Villalta scores, no significant difference in outcome was observed at 6 months (median score, 3; range, 0-15 months; P = .95) or at 1 year (median score, 1; range, 0-15; P = .84). Cumulative patency at 1 year was found to be 64% in postpartum women compared with 93% in nonpostpartum women. The postpartum state was found to be a significant predictor of cumulative patency loss (hazard ratio, 0.10; 95% confidence interval, 0.02-0.62; P = .01). However, no significant difference in primary and primary-assisted patency was observed. Of the postpartum patients, 55% required reintervention (6/11) compared with 29% of nonpostpartum patients (20/68). The mean time to initial reintervention was 62 days (range, 7-233 days). Reintervention was unsuccessful in all cases presenting with 100% vessel occlusion (4/11), but successful in both cases with partial occlusion (2/11). Analysis of the etiologic factors associated with reintervention revealed that all reintervention cases were associated with technical failure to fully lyse and stent beyond residual disease at the initial procedure. No technical, flow, or hematologic factors were identified in the four cases that retained primary patency. CONCLUSIONS This study suggests that percutaneous intervention to achieve early thrombus removal and venous stenting provides a favorable alternative to conservative therapies owing to its potential to decrease the severity of PTS. Completion of lysis and adequate stenting of disease is essential to prevent reocclusion, for which reintervention carries a lower likelihood of success. Further research is warranted to further characterize the appropriate management of postpartum women with iliofemoral DVT.

['Lestak|Katalin|K|', 'Pouncey|Anna Louise|AL|', 'Gwozdz|Adam|A|', 'Silickas|Justinas|J|', 'Fiengo|Leslie|L|', 'Johnson|Oscar|O|', 'Smith|Alberto|A|', 'Saha|Prakash|P|', 'Hunt|Beverley|B|', 'Nelson-Piercy|Catherine|C|', 'Robinson|Susan|S|', 'Breen|Karen|K|', 'Black|Stephen|S|']

2020

29989207

Comparison of Enterprise Point-of-Care and Nova Biomedical Critical Care Xpress analyzers for determination of arterial pH, blood gas, and electrolyte values in canine and equine blood.

BACKGROUND Point-of-care analyzers can provide a rapid turnaround time for critical blood test results. Agreement between the Enterprise Point-of-Care (EPOC) and bench-top laboratory analyzers is important to determine the clinical reliability of the EPOC. OBJECTIVES The aim of the study was (1) to evaluate the precision (repeatability) of blood gas values measured by the EPOC and (2) to determine the level of agreement between the EPOC and Nova Critical Care Express (Nova CCX) for the assessment of arterial pH, blood gases, and electrolyte variables in canine and equine blood. METHODS Arterial blood samples from dogs were analyzed on the EPOC and Nova CCX analyzers to determine precision and agreement of pH, PaCO2 , PaO2 , and HCT. The same analytes plus Na+ , K- , and Cl- were analyzed for agreement using equine blood. Statistical analyses included assessment of precision using the coefficient of variation (CV%), and agreement using the Deming regression, Pearson correlation, and Bland-Altman plots. RESULTS Both analyzers provided precise results of pH, PaCO2 , PaO2, and HCT, meeting CV% quality requirement values. In both species, Deming regression results were acceptable and correlation values were above 0.93 for arterial pH and blood gases, but lower for sodium and chloride. Bland-Altman plots demonstrated varying degrees of bias, but good agreement between the 2 analyzers was seen when arterial blood gases and electrolytes were measured, except for PaCO2 and Cl-. CONCLUSION: The EPOC analyzer provides consistent, reliable results for canine arterial blood gas values and for equine arterial blood gas and electrolyte values. Cl- results could be acceptable with the application of a correction factor, but the PaCO2 results were more variable.

['Elmeshreghi|Taher N|TN|', 'Grubb|Tamara L|TL|', 'Greene|Stephen A|SA|', 'Ragle|Claude A|CA|', 'Wardrop|Jane A|JA|']

2018

24008186

Opioid withdrawal signs and symptoms in children: frequency and determinants.

OBJECTIVES The purpose of this study was to, in a pediatric population, describe the frequency of opioid withdrawal signs and symptoms and to identify factors associated with these opioid withdrawal signs and symptoms. BACKGROUND Opioids are used routinely in the pediatric intensive care population for analgesia, sedation, blunting of physiologic responses to stress, and safety. In children, physical dependence may occur in as little as 2-3 days of continuous opioid therapy. Once the child no longer needs the opioid, the medications are reduced over time. METHODS A prospective, descriptive study was conducted. The sample of 26 was drawn from all patients, ages 2 weeks to 21 years admitted to the Children's Hospital of Richmond pediatric intensive care unit (PICU) and who have received continuous infusion or scheduled opioids for at least 5 days. Data collected included: opioid withdrawal score (WAT-1), opioid taper rate (total dose of opioid per day in morphine equivalents per kilogram [MEK]), pretaper peak MEK, pretaper cumulative MEK, number of days of opioid exposure prior to taper, and age. RESULTS Out of 26 enrolled participants, only 9 (45%) had opioid withdrawal on any given day. In addition, there was limited variability in WAT-1 scores. The most common symptoms notes were diarrhea, vomit, sweat, and fever. CONCLUSIONS For optimal opioid withdrawal assessments, clinicians should use a validated instrument such as the WAT-1 to measure for signs and symptoms of opioid withdrawal. Further research is indicated to examine risk factors for opioid withdrawal in children.

['Fisher|Deborah|D|', 'Grap|Mary Jo|MJ|', 'Younger|Janet B|JB|', 'Ameringer|Suzanne|S|', 'Elswick|R K|RK|']

2013

27354870

Intermuscular pocket for subcutaneous implantable cardioverter defibrillator: Single-center experience.

The subcutaneous implantable cardioverter defibrillator (S-ICD) is a novel device now accepted in clinical practice for treating ventricular arrhythmias. In 14 consecutive patients, S-ICD devices were placed in the virtual space between the anterior surface of the serratus anterior muscle and the posterior surface of the latissimus dorsi muscle. During a mean follow up of 9 months, no dislocations, infections, hematoma formations, or skin erosions were observed. Intermuscular implantation of the S-ICD could be a reliable, safe, and appealing alternative to the standard subcutaneous placement.

['Ferrari|Paola|P|', 'Giofrè|Fabrizio|F|', 'De Filippo|Paolo|P|']

2016

29556196

Curcumin Prevents Acute Neuroinflammation and Long-Term Memory Impairment Induced by Systemic Lipopolysaccharide in Mice.

Systemic lipopolysaccharide (LPS) induces an acute inflammatory response in the central nervous system (CNS) ("neuroinflammation") characterized by altered functions of microglial cells, the major resident immune cells of the CNS, and an increased inflammatory profile that can result in long-term neuronal cell damage and severe behavioral and cognitive consequences. Curcumin, a natural compound, exerts CNS anti-inflammatory and neuroprotective functions mainly after chronic treatment. However, its effect after acute treatment has not been well investigated. In the present study, we provide evidence that 50 mg/kg of curcumin, orally administered for 2 consecutive days before a single intraperitoneal injection of a high dose of LPS (5 mg/kg) in young adult mice prevents the CNS immune response. Curcumin, able to enter brain tissue in biologically relevant concentrations, reduced acute and transient microglia activation, pro-inflammatory mediator production, and the behavioral symptoms of sickness. In addition, short-term treatment with curcumin, administered at the time of LPS challenge, anticipated the recovery from memory impairments observed 1 month after the inflammatory stimulus, when mice had completely recovered from the acute neuroinflammation. Together, these results suggest that the preventive effect of curcumin in inhibiting the acute effects of neuroinflammation could be of value in reducing the long-term consequences of brain inflammation, including cognitive deficits such as memory dysfunction.

['Sorrenti|Vincenzo|V|', 'Contarini|Gabriella|G|', 'Sut|Stefania|S|', "Dall'Acqua|Stefano|S|", 'Confortin|Francesca|F|', 'Pagetta|Andrea|A|', 'Giusti|Pietro|P|', 'Zusso|Morena|M|']

2018

28644568

Treatment of avoidant/restrictive food intake disorder in a cohort of young patients in a partial hospitalization program for eating disorders.

OBJECTIVE Avoidant/restrictive food intake disorder (ARFID) is a recently named condition to classify patients who present with restricted nutritional intake without body image distortion or fear of weight gain. We sought to compare treatment outcomes of patients with ARFID in a family-centered partial hospital program (PHP) to those with other eating disorders (ED). METHOD A retrospective chart review of 130 patients 7-17 years of age admitted to the program from 2008 to 2012 was performed. Intake and discharge data included: length of stay; percentage median body mass index (%MBMI); and scores on the Children's Eating Attitudes Test (ChEAT) and Revised Children's Manifest Anxiety Scale (RCMAS). Between and within group effects were measured for intake and discharge data. RESULTS Patients with ARFID spent significantly fewer weeks in program than those with anorexia nervosa (AN) and experienced a similar increase in %MBMI as patients with AN and other specified/unspecified feeding and eating disorders. All patients exhibited significant improvements in psychopathology over the course of treatment as measured by scores on the ChEAT and RCMAS. DISCUSSION Our findings suggest that patients with ARFID can be successfully treated in the same PHP as patients with other ED, with comparable improvements in weight and psychopathology over a shorter time period. Results are limited to patients with ARFID who exhibit an acute onset of severe food restriction. Future research should incorporate measures relevant to the diagnosis of ARFID and explore how patients with different ARFID subtypes may respond to various treatments.

['Ornstein|Rollyn M|RM|0000-0002-7993-5110', 'Essayli|Jamal H|JH|', 'Nicely|Terri A|TA|', 'Masciulli|Emily|E|', 'Lane-Loney|Susan|S|']

2017

32892708

Multiple species of distinctiveness in memory? Comparing encoding versus statistical distinctiveness on recognition.

The distinctiveness effect refers to the memorial benefit of processing unique or item-specific features of a memory set relative to a non-distinctive control. Traditional distinctiveness effects are accounted for based on qualitative differences in how distinctive items are encoded and subsequently retrieved. This study evaluates whether a separate species of distinctiveness - statistical distinctiveness - may provide an additional benefit to memory beyond traditional task-based processes. Statistical distinctiveness refers to the relative frequency with which a specific memory item or set is processed. The current study examined the presence of statistical distinctiveness through a series of levels-of-processing mixed groups in which related lists were studied using two of the following three tasks to promote either shallow ("E" identification), neutral (reading silently), or deep/distinctive (pleasantness ratings) processing followed by a recognition test. Participants studied lists in which these tasks were used frequently (80% of lists), equally (50% of lists), or infrequently (20% of lists). No recognition advantage was found when tasks were completed infrequently versus frequently. Instead, recognition was greatest for the deeper/more distinctive task - a pattern consistent with an encoding but not a statistical distinctiveness account.

['Gretz|Matthew R|MR|', 'Huff|Mark J|MJ|']

2020

29550238

Cost-Effectiveness Analysis of Nasal Continuous Positive Airway Pressure Versus Nasal High Flow Therapy as Primary Support for Infants Born Preterm.

OBJECTIVE To compare the cost-effectiveness of 2 common "noninvasive" modes of respiratory support for infants born preterm. STUDY DESIGN An economic evaluation was conducted as a component of a multicenter, randomized control trial from 2013 to 2015 enrolling infants born preterm at ≥28 weeks of gestation with respiratory distress, <24 hours old, who had not previously received endotracheal intubation and mechanical ventilation or surfactant. The economic evaluation was conducted from a healthcare sector perspective and the time horizon was from birth until death or first discharge. The cost-effectiveness of continuous positive airway pressure (CPAP) vs high-flow with "rescue" CPAP backup and high-flow without rescue CPAP backup (as sole primary support) were analyzed by using the hospital cost of inpatient stay in a tertiary center and the rates of endotracheal intubation and mechanical ventilation during admission. RESULTS Hospital inpatient cost records for 435 infants enrolled in all Australian centers were obtained. With "rescue" CPAP backup, an incremental cost-effectiveness ratio was estimated of A$179 000 (US$123 000) per ventilation avoided if CPAP was used compared with high flow. Without rescue CPAP backup, cost per ventilation avoided was A$7000 (US$4800) if CPAP was used compared with high flow. CONCLUSIONS As sole primary support, CPAP is highly likely to be cost-effective compared with high flow. Neonatal units choosing to use only one device should apply CPAP as primary respiratory support. Compared with high-flow with rescue CPAP backup, CPAP is unlikely to be cost-effective if willingness to pay per ventilation avoided is less than A$179 000 (US$123 000).

['Huang|Li|L|', 'Roberts|Calum T|CT|', 'Manley|Brett J|BJ|', 'Owen|Louise S|LS|', 'Davis|Peter G|PG|', 'Dalziel|Kim M|KM|']

2018

31287424

A prospective analysis of touch preparation cytology for intraoperative detection of mediastinal lymph node metastases.

INTRODUCTION Patients diagnosed with lung cancer may require immediate evaluation of mediastinal lymph nodes to determine treatment plan. Typically, frozen section (FS) analysis has been used, but this analysis can be time-consuming and uses more tissue than touch preparation (TP) cytologic analysis. TP accuracy has been studied in other organs, but no prospective studies comparing TP to FS have been performed on mediastinal lymph nodes in lung cancer. Our goal was to compare the accuracy of TP to FS in these cases. MATERIALS AND METHODS After obtaining institutional review board approval, all patients undergoing mediastinal lymph node evaluation for a diagnosis of lung cancer were asked to participate. If consent was given, TP and FS analyses were performed on all mediastinal lymph node stations in all patients and compared to permanent hematoxylin and eosin analysis. Data were collected prospectively. RESULTS Twenty patients were enrolled. Mean age was 67.7 years. Fifty-five percent (11 of 20) of patients were men. The mean number of lymph node stations sampled in each patient was 3.4. In predicting the stage of the patient, TP had a sensitivity and specificity of 95% and 100%, respectively. FS had a lower sensitivity, 85%, and a specificity of 100%. On permanent analysis, metastatic foci ranged in size from 0.15 mm to 1.5 mm. CONCLUSIONS TP was more sensitive than FS in detecting mediastinal lymph node metastases. The technical difficulty of obtaining full-thickness sections without creating significant artifact may contribute to the lower sensitivity of FS in detecting micrometastases.

['Wenholz|Alison|A|', 'Xu|Xiao-Meng|XM|', 'Nawgiri|Ranjana|R|', 'Okereke|Ikenna|I|']

2019