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Društvo za jezičke resurse i tehnologije 13

[ "Nestorović, Nataša" ]

[ "Korać, Aleksandra", "Milošević, Verica Lj." ]

[ "2016-01-05T11:47:43", "2020-07-03T08:09:54" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=789", "https://nardus.mpn.gov.rs/handle/123456789/2118", "https://fedorabg.bg.ac.rs/fedora/get/o:7111/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=44653839" ]

[ "Fitoestrogeni iz soje, pre svega genistein i daidzein, su veoma zastupljeni u životu savremenog čoveka, kako kroz ishranu, tako i kroz pomoćna lekovita sredstva. Zbog svog selektivno modulatorskog, tj. tkivno specifičnog estrogenskog delovanja, fitoestrogeni poslednjih godina postaju atraktivno polje intenzivnih studija. Period gašenja reproduktivne funkicije kod žena, tj. menopauza, vrlo često je praćen tegobama i poremećajima koje su posledica smanjenja koncentracije endogenog estradiola, odnosno funkcije ovarijuma. Stoga je jedna od mogućih terapijskih aplikacija genisteina i daidzeina upravo sprečavanje i ublažavanje ovih tegoba, a kao alternativa supstitucionoj hormonskoj terapiji. Međutim, njihovo delovanje na organe ženskog reproduktivnog sistema, naročito u periodu menopauze, još uvek nije u potpunosti istraženo. Cilj ove studije obuhvata rasvetljavanje dejstava fitoestrogena na reproduktivni sistem nezrelih i acikličnih ženki pacova. Od posebnog interesa je jasno definisanje potencijala fitoestrogena u poboljšanju funkcije reproduktivnog sistema u animalnom modelu menopauze, kao i poređenje efekata njihove aplikacije sa onima koje ima aplikacija terapijske doze estradiol dipropionata, koji se koristi kao lek izbora u prevenciji i terapiji menopauzalnih simptoma. Za ispitivanje estrogenske aktivnosti fitoestrogena (genisteina i daidzena) korišćen je uterotrofni esej po protokolu Organizacije za ekonomsku saradnju i razvoj (OECD). Ženkama pacova starosti 19 dana aplikovani su fitoestrogeni (genistein i daidzein), tokom tri dana u tri doze (visokoj, srednjoj i niskoj). U okviru protokola predviđene su i kontrolne grupe - pozitivne kontrolne grupe, koje su primale 17α-etinil estradiol (u dve doze), negativne kontrolne grupe koje su primale rastvarače, kao i intaktna kontrolna grupa. Nakon uterotrofnog eseja, utvrđeno je koja doza fitoestrogena (genisteina i daidzeina) ispoljava minimalan estrogenski efekat. Ta doza je, tokom četiri nedelje, aplikovana acikličnim ženkama pacova starosti 12 meseci, koje predstavljaju eksperimentalni model menopauze. Na ovaj način utvrđeno je da li ispitivani fitoestrogeni (genistein i daidzein) imaju efekat na strukturu i funkciju organa reproduktivnog sistema, hipofize, ovarijuma, uterusa i vagine. Dodatno su efekti fitoestrogena poređeni sa efektima koje u organima reproduktivnog sistema izaziva terapijska doza estradiol dipropionata. Promene u strukturi i funkciji organa reproduktivnog sistema analizirane su histološkim, imunofluorescentnim, savremenim stereološkim, morfometrijskim i molekularno biološkim metodama. Imunofluorescentno obeležavanje specifičnih antigena, omogućilo je vizuelizaciju gonadotropnih i laktotropnih ćelija hipofize, i izračunavanje relativnog intenziteta fluorescentnog signala. Istim metodama, detektovano je i prisustvo laktoferina, kao i steroidnih receptora u uterusu nezrelih i acikličnih ženki pacova..." ]

[ "Soy phytoestrogens, primarily genistein and daidzein, are widely used through diet and as dietary supplements. Since they may act as “natural” selective modulators of estrogen receptor, scientific interest for phytoestrogens is constantly increasing. Cessation of a women’s reproductive function, i.e. menopause, is often accompanied with disorders that are consequence of reduced ovarian function and low estrogen concentration. Therefore, one of the possible therapeutic application of genistein and daidzein is prevention and alleviation of menopausal symptoms, as an alternative to hormone replacement therapy. The aim of this study is to examine the effects of phytoestrogens on the reproductive system of immature and middle-aged female rats. Of particular interest is to define the potential of phytoestrogens for improvement of reproductive system function in an animal model of menopause, and to compare these effects with the effects of therapeutic doses of estradiol dipropionate, commonly used in prevention and treatment of menopausal symptoms. Uterotrophic assay was used in order to test estrogenic activity of genistein and daidzein, according to the protocol of the Organization for Economic Cooperation and Development (OECD). Immature female rats received phytoestrogens (genistein and daidzein), in three doses (high, medium and low) for three consecutive days, from 19th till 21st day of life. According to the protocol, control groups were formed - the positive control groups that received 17α - ethynyl estradiol (in two doses), negative control groups that received solvents, and the intact control group. After uterotrophyc assay, minimal dose of genistein and daidzein that exerts estrogenic effect was determined. This dose was administered to 12 months old, middleaged female rats, for four weeks. After this period it was analyzed whether genistein and daidzein exerted beneficial effects on the structure and function of the pituitary, ovary, uterus and vagina. These effects were then compared with the effects of the therapeutic dose of estradiol dipropionate. Changes in the structure and function of the organs of reproductive system of immature and middle-aged female rats were analyzed by histological, immunofluorescence, design-based stereological, morphometric and molecular biological methods. Immunofluorescent labeling of specific antigens allowed the visualization of the pituitary lactotropic and gonadotropic cells, and the calculation of the relative intensity of fluorescent signal, i.e. concentration of antigen within the cell. In the same manner, the presence of lactoferrin and steroid receptors in the uterus of immature and middle-aged female rats was analysed..." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173009/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "ženski reproduktivni sistem", "fitoestrogeni", "estradiol", "uterotrofni esej", "menopauza" ]

[ "female reproductive system", "phytoestrogens", "estradiol", "uterotrophic essay", "menopause" ]

[ "Morfofunkcionalne osobine reproduktivnog sistema nezrelih i acikličnih ženki pacova nakon tretmana fitoestrogenima iz soje", "Morphofunctional features of reproductive system of immature and middle-aged female rats after treatments with soy phytoestrogens" ]

[ "" ]

[ "BY-NC-ND" ]

[ "http://nardus.mpn.gov.rs/bitstream/id/2199/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/2199/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2118" ]

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[ "Anđus, Pavle" ]

[ "Harhaji-Trajković, Ljubica", "Trajković, Vladimir", "Božić, Biljana" ]

[ "2016-01-05T11:47:40", "2020-07-03T08:10:30" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/2112", "http://eteze.bg.ac.rs/application/showtheses?thesesId=786", "https://fedorabg.bg.ac.rs/fedora/get/o:7104/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024639666" ]

[ "U ovoj doktorskoj disertaciji ispitivan je uticaj inhibicije intracelularnog energetskog senzora protein kinaze aktivirane adenozin-monofosfatom (AMPK) na indukciju apoptoze i autofagije u tumorskim ćelija. Farmakološki inhibitor AMPK dorzomorfin indukovao je G2/M blokadu ćelijskog ciklusa, praćen apoptozom koju karakteriše aktivacija kaspaza, eksternalizacija fosfatidilserina i fragmentacija DNK u U251 humanim i C6 pacovskim ćelijama glioma, dok na vijabilitet primarnih pacovskih astrocita i ćelija mišjeg melanoma B16 nije imao uticaja. Mehanizam indukcije apoptoze dorzomorfinom bio je posredovan stimulacijom produkcije reaktivnih vrsta kiseonika i inhibicijom ekspresije antiapoptotskog Bcl-2 proteina. Dorzomorfin je inhibirao fosforilaciju i enzimatsku aktivnost AMPK, što je za posledicu imalo smanjenje fosforilacije njenog supstrata acetil-CoA karboksilaze. Aktivatori AMPK metformin i AICAR delimično su neutralisali blokadu ćelijskog ciklusa, oksidativni stres i apoptozu indukovanu dorzomorfinom. Mala interferirajuća RNK (siRNK) koja sprečava ekspresiju humanog AMPK enzima je poput dorzomorfina zaustavila proliferaciju ćelija u G2/M fazi ćelijskog ciklusa, ali nije izazvala oksidativni stres i apoptozu u U251 ćelijama. Dakle, inhibicija AMPK je neophodna, ali ne i dovoljna za indukciju apotpoze dorzomorfinom u ćelijama glioma. U ovoj studiji takođe je pokazano da dorzomorfin indukuje autofagiju u ćelijama kancera. Indukcija autofagije u U251 ćelijama detektovana je fluorescentnim bojenjem unutarćelijskih kiselih vezikula akridin oranžom, indukcijom beklina-1, degradacijom p62 proteina i konverzijom LC3-I u formu asociranu sa autofagozomima LC3-II u odsustvu i prisustvu proteolitičkih inhibitora..." ]

[ "In this doctoral dissertation the effect of intracellular energy sensor AMP-activated protein kinase (AMPK) inhibition on induction of apoptosis and autophagy in tumor cells was investigated. Pharmacological AMPK inhibitor dorsomorphin caused G2/M cell cycle block, accompanied by apoptotic cell death characterized by caspase activation, phosphatidylserine exposure and DNA fragmentation in U251 human and C6 rat glioma cells, while it had no effect on viability of primary rat astrocytes and B16 mouse melanoma cells. The mechanisms underlying the pro-apoptotic action of dorsomorphin involved induction of oxidative stress and down-regulation of antiapoptotic molecule Bcl-2. Dorsomorphin diminished AMPK phosphorylation and enzymatic activity, resulting in reduced phosphorylation of its target acetyl CoA carboxylase. AMPK activators metformin and AICAR partly prevented the cell cycle block, oxidative stress and apoptosis induced by dorsomorphin. The small interfering RNA (siRNA) targeting of human AMPK mimicked dorsomorphin-induced G2/M cell cycle arrest, but failed to induce oxidative stress and apoptosis in U251 glioma cells. Therefore, AMPK inhibition is required, but not sufficient for dorsomorphin-mediated apoptotic death of glioma cells. In this study, it was also reported that dorsomorphin can induce autophagy in cancer cells. The induction of autophagy in U251 human glioma cell line was demonstrated by acridine orange staining of intracellular acidic vesicles, Beclin 1 induction, p62 decrease and conversion of LC3-I to autophagosome-associated LC3-II in the absence and presence of proteolysis inhibitors. The presence of autophagosome like vesicles was confirmed by transmission electron microscopy. Dorsomorphin-mediated inhibition of AMPK and Raptor in U251 cells was associated with paradoxical decrease in phosphorylation of AMPK/Raptor-repressed mTOR, a major negative regulator of autophagy, and its downstream target p70S6K..." ]

[ "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41025/RS//", "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173053/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "gliomi", "dorzomorfin", "AMPK", "apoptoza", "oksidativni stres", "autofagija", "kancer", "Akt", "mTOR" ]

[ "glioma", "dorsomorphin", "AMPK", "apoptosis", "oxidative stress", "autophagy", "cancer", "Akt", "mTOR" ]

[ "Uloga inhibicije protein-kinaze aktivirane adenozin-monofosfatom u indukciji apoptoze i autofagije u tumorskim ćelijskim linijama", "The role of adenosine monophosphate-activated protein kinase inhibition in apoptosis and autophagy induction in tumor cell lines" ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/2321/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2321/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2112" ]

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[ "Stupar, Slobodan" ]

[ "Simonović, Aleksandar", "Maksimović, Stevan" ]

[ "2016-01-05T12:01:45", "2020-07-03T08:40:53" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=413", "https://nardus.mpn.gov.rs/handle/123456789/2298", "https://fedorabg.bg.ac.rs/fedora/get/o:6131/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=514011299" ]

[ "Предмет истраживања у докторској дисертацији је процена преосталог века структура са иницијалним оштећењима под дејством спектра термо- механичких оптерећења. Прво су проучаване структуре са иницијалним оштећењима само под дејством термичког оптерећења, и за задате граничне услове одређивани су параметри механике лома. За поуздану процедуру одређивања параметара механике лома где се превасходно мисли на фактор интезитета напона коришћене су нумеричке методе и теоретске поставке линеарно еластичне механике лома. Структуре које су проучаване се углавном односе на делове ваздухоплова који су од виталног значаја за њихов исправан и поуздан рад. Анализирани су делови поља оплате са геометријским дисконтинуетима и иницијалним оштећењима под дејством термичког оптерећења. Такође су анализирани делови погонских група код ваздухоплова. Посебно је проучаван део лопатица код млазних мотора којима је на самом крају нанет слој термичке заштите због високих радних температура. Детаљна анализа структуре у граничном слоју између основног слоја лопатице и термичке заштите, када долази до појаве напрслина може имати значаја у самој фази пројектовања као и у току експлоатације погонских група код ваздухоплова. Још једном делу структуре погонских група код ваздухоплова посвећена је посебна пажња у анализи са аспекта механике лома у оквиру докторске дисертације. Делови везе диска ротора и корена лопатице турбине представљају најоптерећеније делове структуре. У даљим истраживањима у оквиру докторске дисертације, детаљњо је анализиран структурални елемент са површинском напрслином изложен механичким и термичким оптерећењима. За раличиту геометрију површинске напрслине и различите нивое механичог оптерећења и температурних градијената, израчунате су вредности фактора интезитета напона. Одређивање функционалне зависност фактора интезитета напона у односу на положај врха напрслине за различите геометрије облика напрслине, представља значајна сазнања о понашању таквих структура са аспекта механике лома. Такође, код ваздзхопловних конструкција сусрећемо се са деловима структура који су спојени заваривањем. Као последица процеса заваривања јављају се заостали напони који могу неповољно утицати на понашање структуре под дејством динамичког оптерећења и стога је у оквиру докторске дисертације посвећена посебна пажња одређивању заосталих напона применом методе коначних елемената. Прецизно одређена расподела заосталих напона као и њихов интезитет могу значајно смањити преостали век структуре са аспеката замора материјала." ]

[ "The subject of doctoral dissertation is research into the residual life of structures with initial damages under the influence of the spectrum of thermo-mechanical loads. First, the structures with initial damages under the influence of thermal load only were studied, and for given boundary conditions the parameters of fracture mechanics were determined. For a reliable procedure of determining fracture mechanics parameters, which primarily refers to the stress intensity factor, numerical methods and theoretical approaches of linear elastic fracture mechanics were used. The structures that were studied are mainly related to aircraft parts that are vital for their proper and safe operation. The parts of the plate with geometric discontinuities and initial damages under the influence of thermal load were analyzed. The components of power engine of aircraft were also analyzed. In particular, a part of the blades in jet engines was researched, at the end of which the layer of thermal protection was applied due to high operating temperatures. The detailed analysis of structures in the boundary layer between the basic blade and thermal protection when cracks appear can be very important in the design phase and during the operation of aircraft power engines. In this doctoral dissertation special attention was devoted to one more part of the power engine structure of aircraft, in the analysis in terms of fracture mechanics. The parts of the connection between the turbine rotor and the turbine blade root are the most overload parts of the structure In the further research in this doctoral dissertation, structural element with a surface crack subjected to mechanical and thermal loads was analyzed. For various geometry of surface crack and different levels of mechanical loads and temperature gradients, the values of stress intensity factor were calculated. Determination of the functional dependence of the stress intensity factors in relation to the position of crack tip for different types of crack geometry represents significant knowledge about the behavior of such structures in terms of fracture mechanics. Also, the aircraft constructions encounter with parts of structures that are connected by welding. As the result of welding processes, the residual stresses that can adversely affect the behavior of structure under dynamic loading occur, and therefore, in this doctoral dissertation special attention is devoted to the determination of residual stresses using finite element method. Precisely determined distribution of residual stresses, as well as their intensity can significantly reduce the residual life of structures from the aspects of fatigue life of material." ]

[ "" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc/4.0/" ]

[ "термо-механичка оптерећења", "напрслина", "фактор интезитета напона", "метода коначних елемената", "преостали век" ]

[ "thermo-mechanical loads", "crack", "stress intensity factors", "finite element method", "residual life" ]

[ "Процена преосталог века структуралних елемената са иницијалним оштећењима под дејством спектра термомеханичких оптерећења", "The assessment of residual life of structural elements with an initial damage under thermomechanical loading spectrum" ]

[ "" ]

[ "BY-NC" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/6960/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/6960/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2298" ]

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[ "Mišić, Danijela" ]

[ "Sabovljević, Aneta", "Avramov, Stevan", "Simonović, Ana" ]

[ "2016-01-05T11:47:59", "2020-07-03T08:10:10" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=1271", "https://nardus.mpn.gov.rs/handle/123456789/2134", "https://fedorabg.bg.ac.rs/fedora/get/o:2077/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=" ]

[ "Kičicа (Centaurium erythraea Rafn) је fаrmаkоlоški аtrаktivnа bilјnа vrstа kоја pоkаzuје čitаv spеktаr biоlоških аktivnоsti, а čiјi је biоdivеrzitеt nеdоvоlјnо istrаžеn. Оsnоvni cilј prеdstаvlјеnоg istrаživаnjа јеstе аnаlizа gеnеtičkоg i fitоhеmiјskоg divеrzitеtа kičicе na teritoriji Balkanskog poluostrva. U tоm smislu, fоrmirаnа је kоlеkciја sеmеnа kičicе pоrеklоm iz prirоdnih pоpulаciја, kоја оslikаvа divеrzitеt vrstе nа оvоm pоdručјu. Istrаživаnjа su uklјučilа 43 pоpulаciје оvе vrstе, оd kојih је 41 pоrеklоm sа Bаlkаnskоg pоluоstrvа. Zа prоcеnu gеnеtičkоg divеrzitеtа оptimizоvаnе su tеhnikе RAPD (Random Amplified Polymorphic DNA) i TRAP (Target Region Amplification Polymorphism) mоlеkulаrnih mаrkеr sistеmа. DNK upоtrеblјеnа u оvim аnаlizаmа је izоlоvаnа iz bilјаkа gајеnih u uslоvimа in vitro, kаkо bi sе оbеzbеdilа аutеntičnоst DNK kičicе i еliminisаlа mоgućnоst kоntаminаnаciје strаnоm DNK. Zа pоtrеbе dоbiјаnjа štо vеćеg brоја infоrmаtivnih јеdinicа, upоtrеblјеnо је 16 dеsеtоmеrnih prајmеrа u slučајu RAPD mаrkеrа, pri čеmu је dоbiјеnо ukupnо 408 rаzličitih PCR prоdukаtа, dоk su zа TRAP mаrkеrе upоtrеblјеnе kоmbinаciје tri spеcifičnа i tri аrbitrаrnа prајmеrа i dоbiјеnо је 767 frаgmеnаtа DNK. Spеcifični prајmеri kоrišćеni u TRAP аnаlizаmа, kоnstruisаni su nа оsnоvu sеkvеnci RNK kičicе sа visоkim stеpеnоm hоmоlоgiје sа DNK sеkvеncаmа zа slеdеćе еnzimе: gеrаniоl 10-hidrоksilаzа, citоhrоm P450 rеduktаzа i sеkоlоgаnin sintаzа, оbјаvlјеnih u еlеktrоnskim bаzаmа pоdаtаkа…" ]

[ "Centaury (Centaurium erythraea Rafn) is a medicinal and pharmacologically attractive plant species which has a wide range of biological activity. The main objective of presented study was to estimate genetic and phytochemical diversity of centaury within the Balkan Peninsula. With that purpose, seed collection of natural centaury populations was established. The study included 43 populations of C. erythraea, among which 41 originated from the Balkan Peninsula. Molecular marker techniques, such as RAPD (Random Amplification Polymorphic DNA) and TRAP (Target Region Amplification Polymorphism) were optimized to investigate the genetic diversity among these populations. DNA used in these analyses originated from in vitro grown plants to ensure the authenticity of centaury DNA by eliminating the possibility of its contamination by extraneous DNA. To acquire sufficient informational characters, 16 decameric primers were applied in RAPD assays, which produced 408 different PCR products, while TRAP assays included the combinations of 3 specific and 3 arbitrary primers, resulting in 767 fragments of DNA. Specific primers used for TRAP analyses were constructed according to the RNA sequences, showing high homology with DNA sequences published in electronic data bases for following enzymes: geraniol 10-hydroxylase, cytochrome P450 reductase and secologanin synthase. Molecular markers provided a high percentage of polymorphism..." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173024/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc/4.0/" ]

[ "Centaurium erythraea", "Balkansko poluostrvo", "fragmentacija staništa", "populacije", "molekularni markeri", "RAPD", "TRAP", "genetički varijabilnost", "sekoiridoidni glikozidi" ]

[ "Centaurium erythraea", "Balkan Peninsula", "habitat fragmentation", "populations", "molecular markers", "RAPD", "TRAP", "genetic variability", "secoiridoid glycosides" ]

[ "Fitohemijska i molekularno-genetička karakterizacija populacija kičice (Centaurium erythraea Rafn) sa Balkanskog poluostrva", "Phytochemical and molecular characterization of centaury (Centaurium erythraea Rafn) populations from the Balkan Peninsula" ]

[ "" ]

[ "BY-NC" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/2254/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2254/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2134" ]

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[ "Kalezić, Miloš" ]

[ "Ivanović, Ana", "Jojić, Vida" ]

[ "2016-01-05T11:47:40", "2020-07-03T08:09:50" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=794", "https://nardus.mpn.gov.rs/handle/123456789/2114", "https://fedorabg.bg.ac.rs/fedora/get/o:7119/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=44660495" ]

[ "Integracija, modularnost i razvojna ograničenja predstavljaju važne komponente fenotipske varijabilnosti. U ovoj tezi analiziran je uticaj ovih komponenti na morfološku evoluciju ekstremiteta pet vrsta velikih krestatih mrmoljaka. Veliki krestasti mrmoljci predstavljaju važne model organizme za istraživanja u oblasti morfološke integracije i razvojnih ograničenja, posebno ako se analiziraju ekstremiteti. To je grupa blisko srodnih vrsta sa poznatim filogenetskim odnosima, i izraženom vezom izmedju morfološke forme/tipa (oblika tela i dužine ekstremiteta) i preference prema akvatičnoj/terestričnoj sredini. Akvatične vrste se odlikuju izduženim telom i kratkim nogama, dok su vrste sa više terestričnim životnim stilom snažnije i robusnije građe sa dugim nogama. Takođe, kao grupa sa složenim životnim ciklusom, gde larveni, juvenilni i adultni stupanj imaju različite morfologije, ekološke sredine i preference, načine ishrane i lokomocije, veliki krestasti mrmoljci imaju posebno mesto u izučavanju ontogenetskih putanja tokom razvića. Ontogenetska promena niše i tranzicija između akvatične i terestrične sredine je povezana sa procesom metamorfoze. Iako ekstremiteti mrmoljaka ne prolaze kroz drastične promene strukture tokom tog procesa, funkcionalni zahtevi za efikasnom lokomocijom u te dve sredine su bitno različiti. Pretpostavka je da razlike između vrsta u pogledu stepena korišćenja akvatične tj. terestrične lokomocije, može biti faktor koji doprinosi razlikama u dužinama skeletnih elemenata i različitim obrascima korelacija između elemenata ekstremiteta, oslikavajući adaptacije na različite uslove sredine i način života (više ili manje akvatičan-terestričan)..." ]

[ "Integration, modularity and developmental constraints are three related components of phenotypic variability. Their impacts on morphological evolution on limbs in five species of crested newts were analyzed in this thesis. Crested newts are valuable model or ganisms in studies of morphological integration and developmental constraints, especially when concern limbs. They are closely related group of species with known phylogenetic relations, and each characterized by a specific body-to-limb conformation associated with their respective ecologies. Aquatic species of newts have small, elongate bodies and small limbs while more terrestrial species are with large, stocky bodies and robust limbs. Also, as group with complex life cycle, where larval, juvenile and adult stage have different morphologies, ecol ogies and different diet and locomotor preferences, crested newts have special pl ace in studies of changes in ontogenetic trajectories. Ontogenetic niche change and transition between aquatic and terrestrial environment is connected with process of metamorphosis. Although limbs in newts do not undergo drastic chan ges in structure during that process, functional demands for high efficient loco motion in both environments are quit different. It is assumed that the differenc es between species in degree of aquatic and terrestrial locomotion may be the fa ctor which can attribute to changes in length of skeletal elements and differe nt patterns of correlations between limb elements, reflecting adaptations on diffe rent environments and life styles of analyzed species of crested newts..." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173043/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "ekstremiteti", "veliki krestasti mrmoljci", "morfološka integracija i modularnost", "sekvencijalna heterohronija", "alometrijska heterohronija" ]

[ "limbs", "crested newts", "morphological integration and modularity", "sequence heterochrony", "allometric heterochrony" ]

[ "Evolucija ekstremiteta velikih mrmoljaka (Triturus cristatus complex, Salamandridae, Caudata)", "Limb evolution in crested newts (Triturus cristatus complex, Salamandridae, Caudata)" ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/2180/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2180/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2114" ]

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[ "" ]

[ "Radović, Svetlana" ]

[ "Kondić-Šipka, Ankica", "Maksimović, Vesna" ]

[ "2020-07-03T08:11:38" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/4173", "http://eteze.bg.ac.rs/application/showtheses?thesesId=2317", "https://fedorabg.bg.ac.rs/fedora/get/o:10230/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024960946" ]

[ "Uvod: Hlebna pšenica (Triticum aestivum L.) je jedna od najvažnijih žitarica široko zastupljena u proizvodnji u svetu, odnosno po ukupnoj proizvodnji zrna nalazi se na trećem mestu iza kukuruza i pirinča. Zahvaljujući značaju ove biljne vrste posebno u ljudskoj ishrani, priporitet u selekciji je usmeren na poboljšanje postojećih i stvaranje novih sorti sa povećanim genetičkim potencijalom za prinos, kako bi se zadovoljile i obezbedile kvantitativne potrebe za ovom žitaricom. Razvoj savremenih metoda i primena najnovijih tehnologija iz oblasti biotehnologije, sa posebnim akcentom na korišćenje molekularnih markera, otvorila je mogućnost implementacije molekularnog oplemenjivanja u klasične oplemenjivačke programe. Na ovaj način se putem marker-asistirane selekcije ostvaruje efikasnije, preciznije i ekonomičnije stvaranje prinosnijih sorti. Najvažnije agronomske osobine nalaze se pod kontrolom većeg broja gena i lokusa za kvantitativna svojstva (QTL), te su i osobine klasa koje predstavljaju najvažnije komponente prinosa, svojstva koja se nalaze pod većim uticajem faktora spoljašnje sredine. Danas se za otkrivanje i precizno definisanje hromozomskih regiona odgovornih za ekspresiju ovih važnih osobina primenjuje jedan od najperspektivnijih pristupa tzv. asocijativno mapiranje koje visokom rezolucijom mapiranja olakšava primenu marker-asistirane selekcije. Cilj istraživanja je bio da se utvrde osnovni parametri genetičke varijabilnosti primenom mikrosatelita, da se proceni genetička struktura u ispitivanoj populaciji na osnovu molekularnih podataka, da se analiziraju svojstva klasa kao najznačajnijih komponenti prinosa pšenice kao i adaptivnih svojstava tokom pet vegetacionih sezona. Takođe, postavljen je i cilj da se ispita neravnoteža vezanosti između hromozoma, i da se analiziraju veze između markera i svojstava u svim ispitivanim godinama primenom najsavremenijeg pristupa - asocijativnog mapiranja. Krajnji cilj bio je da se identifikuju značajni i stabilni lokusi odgovorni za ekspresiju pojedinačnih karakteristika klasa i svojstava adaptabilnosti i da se utvrde poželjni aleli u okviru lokusa koji su imali najveći uticaj na fenotipsku varijabilnost ispitivanih osobina..." ]

[ "Introduction: Bread wheat (Triticum aestivum L.) is one of the most important cereals worldwide and in terms of total grain production wheat is positioned on third place after maize and rice. Due to a great importance for human diet, the priority in wheat breeding is focused on improving of existing and creating new varieties with increased genetic yield potential, in order to meet quantitative demands for this grain. The development of modern methods and the use of new technologies in the field of biotechnology, with particular emphasis on the use of molecular markers, opened the possibility of implementing molecular breeding in classical breeding programs. In this way, the creation of high yielding varieties is more efficient, accurate and cost-effective. The most important agronomic traits are controlled by a large number of genes and quantitative trait loci (QTL). As the most important yield components, spike-related traits are under strong influence of numerous environmental factors. In the last few years, association mapping has been considered as one of the most promising methods for the exploration of precise detection chromosome regions responsible for the expression of these important traits. This high resolution perspective approach facilitates the application of marker-assisted selection into breeding process. The aim of this study was to determine the basic parameters of genetic variability by using microsatellites, to assess the genetic structure in the population based on molecular data, to analyze the spike characteristics as the most important yield components and the adaptability traits during five growing seasons. Moreover, linkage disequilibrium, and the associations between SSR markers and traits investigated were examined by use of association mapping. Furthermost, the aim was to identify significant and stable loci responsible for expression of spike-related and adaptability characteristics, to identify desirable alleles within loci that have had the greatest impact on the phenotypic variability..." ]

[ "info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31066/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]

[ "mikrosatelitski markeri", "hlebna pšenica", "genetički diverzitet", "lokusi za kvantitatitvna svojstva - QTL", "komponente prinosa", "karakteristike klasa", "adaptabilne osobine", "GLM i MLM pristupi" ]

[ "microsatellite markers", "bread wheat", "genetic diversity", "quantitative trait loci - QTL", "yield components", "spike characteristics", "adaptability traits", "GLM and MLM approaches" ]

[ "Molekularna i asocijativna analiza svojstava klasa pšenice (Triticum aestivum L.)", "Molecular and association analysis of spike-related traits in wheat (Triticum aestivum L.)" ]

[ "" ]

[ "BY-NC-SA" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/2561/Ljiljana_Brbaklic_Referat_BF.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2561/Ljiljana_Brbaklic_Referat_BF.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2560/Disertacija81.pdf", "https://nardus.mpn.gov.rs/bitstream/id/2560/Disertacija81.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_4173" ]

[ "" ]

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[ "Savić-Pavićević, Dušanka" ]

[ "Juranić, Zorica", "Vajs, Vlatka", "Aljančić, Ivana", "Šavikin, Katarina" ]

[ "2016-01-05T11:47:41", "2020-07-03T08:10:24" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=885", "https://nardus.mpn.gov.rs/handle/123456789/2115", "https://fedorabg.bg.ac.rs/fedora/get/o:7290/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024607410" ]

[ "Bioaktivni sastojci biljnih vrsta nalaze se u centru pažnje istraživanja u savremenoj onkologiji zbog svoje moguće uloge u hemioprevenciji, odnosno inhibiciji različitih koraka procesa maligne transformacije. Antikancerski potencijal biljnih jedinjenja zasniva se na mogućnosti redukcije slobodnih radikala, regulacije karcinogen-aktivirajućih i karcinogen-detoksifikujućih enzima, kao i mogućnosti inhibicije inflamatornih citokina, zatim na mogućnosti da dovedu do promena u regulaciji faktora rasta i ciljnih molekula signalnih puteva koji kontrolišu ćelijski rast, proliferaciju i apoptozu, kao i angiogenezu, invaziju i metastazu malignih ćelija. Osnovni cilj istraživanja je bio da se ispita citotoksičnost, odnosno odrede intenzitet i mehanizmi citotoksičnog dejstva pet ekstrakata izolovanih u vidu frakcija iz endemične biljne vrste Helichrysum zivojinii Černjavski et Soška prema specifičnom malignom ćelijskom tipu, kao i da se odredi selektivnost u antitumorskom dejstvu prema nizu humanih malignih ćelija, kako u odnosu na poreklo - tip tumora, tako i u odnosu na zdrave mononuklearne ćelije periferne krvi (PBMC). Kako bi se doprinelo boljoj proceni antitumorskog potencijala ekstrakata, ispitan je i uticaj ekstrakata na invazivnost humanih metastatskih malignih ćelija i na angiogenezu endotelijalnih ćelija. Hemijska karakterizacija biljnih ekstrakata je imala za cilj da omogući razumevanje veze između kvalitativnog sastava i intenziteta citotoksične aktivnosti ekstrakata. Citotoksična aktivnost pet ekstrakata endemične biljne vrste Helichrysum zivojinii Černjavski et Soška je ispitana na sledećim humanim malignim ćelijskim linijama: HeLa (adenokarcinom cerviksa), Fem-x (melanom), K562 (mijeloidna leukemija), MDA-MB-361 (adenokarcinom dojke), MDA-MB-231 (adenokarcinom dojke), kao i prema EA.hy926 ćelijama (transformisane humane endotelijalne ćelije umbilikalne vene)..." ]

[ "Bioactive constituents of plants are in the center of attention of modern cancer research due to their prospective role in cancer chemoprevention based on the suppression of different stages in malignant transformation. The anticancer potential of plant compounds could be attributed to their ability to scavenge free radicals, regulate carcinogen-activating and –detoxifying enzymes and inhibit inflammatory cytokines, than to induce changes in the regulation of growth factors and target molecules in oncogenic signal transduction pathways implicated in cell growth, proliferation, apoptosis, as well as in angiogenesis, invasion and metastasis of cancer cells. The aim of this research was to investigate the cytotoxicity, more exactly to determine the intensity and mechanisms of the cytotoxic actions of the five extracts isolated as fractions from the endemic plant species Helichrysum zivojinii Černjavski et Soška against specific malignant cell type, as well as to determine the selectivity in their antitumor actions against malignant cell lines of different tumor origin and against healthy peripheral blood mononuclear cells (PBMC). To fully evaluate the antitumor potential of extracts, the examination of their effects on the invasiveness of human metastatic malignant cells as well as on the angiogenesis of endothelial cells was done. Chemical characterization of plant extracts was performed in order to understand the relation between qualitative composition and intensity of cytotoxic action of the investigated extracts. The cytotoxic activities of the five extracts of the endemic plant species Helichrysum zivojinii Černjavski et Soška were tested against selected human malignant cell lines: HeLa (cervix adenocarcinoma), Fem-x (melanoma), K562 (myelogenous leukemia), MDA-MB-361 (breast adenocarcinoma), MDA-MB-231 (breast adenocarcinoma), as well as against transformed human umbilical vein endothelial EA.hy926 cells..." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175011/RS//", "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172053/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "Helichrysum zivojinii Černjavski et Soška", "citotoksičnost", "maligne ćelije", "normalne PBMC", "apoptoza", "invazivnost", "angiogeneza in vitro" ]

[ "Helichrysum zivojinii Černjavski et Soška", "cytotoxicity", "malignant cells", "normal PBMC", "apoptosis", "invasiveness", "angiogenesis in vitro" ]

[ "In vitro ispitivanje antitumorske aktivnosti ekstrakata endemične biljne vrste Helichrysum zivojinii Černjavski et Soška", "In vitro investigation of antitumor activities of extracts of endemic plant species Helichrysum zivojini Černjavski et Soška U" ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/2301/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2301/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2115" ]

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[ "" ]

[ "" ]

[ "Radović, Svetlana" ]

[ "Duduk, Bojan", "Stamenković-Radak, Marina" ]

[ "2016-01-05T11:46:41", "2020-07-03T08:10:21" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/2080", "http://eteze.bg.ac.rs/application/showtheses?thesesId=165", "https://fedorabg.bg.ac.rs/fedora/get/o:5400/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=43480591" ]

[ "Na teritoriji Republike Srbije su u periodu od 2009. do 2011. godine sakupljeni uzorci biljaka sa simptomima karakterističnim za prisustvo fitoplazmi. Upotrebom PCR metode pomoću univerzalnih prajmera P1/P7 i R16F2n/R16R2 u njima su detektovane fitoplazme. Upotrebom restrikcionih enzima Tru1I, HhaI, RsaI i Tsp509I na R16F2n/R16R2 amplikone, ove fitoplazme su na osnovu dobijenih restrikcionih profila identifikovane kao Aster yellows fitoplazme (ribozomalna grupa 16SrI), podgrupe 16SrI-B, I-C i I-P, odnosno kao Stolbur fitoplazme (ribozomalna grupa 16SrXII). Upotrebom iste PCR-RFLP metode kod 19 sojeva Aster yellows fitoplazmi iz kolekcije i tri soja poreklom iz šargarepe iz Srbije, potvrĎena je pripadnost podgrupama 16SrI-A, I-B, I-C i I-F preuzeta iz literature. TakoĎe je utvrĎena pripadnost odreĎenoj podgrupi kod četiri soja iz kolekcije, koji se prvi put koriste u analizi i o kojima nema literaturnih podataka. Upotrebom novodizajniranih prajmera AYgroesF/AYampR i AYgroelF/AYgroelR umnoţen je groEL gen kod sva 34 testirana soja Aster yellows fitoplazmi. Analizom restrikcionih profila dobijenih pomoću Tru1I i AluI restrikcionih enzima na AYgroelF/R amplikone, utvrĎeno je postojanje šest, odnosno osam različitih profila na osnovu kojih su svi testirani sojevi svrstani u devet groELI podgrupa (groELI-I do groELI-IX). Na osnovu groEL gena podgrupe 16SrI-A i I-C su dalje diferencirane u dve groELI podgrupe, 16SrI-B u tri, dok podgrupe 16SrI-M i I-L nisu pokazale nikakve meĎusobne razlike u odnosu na jedan deo podgrupe 16SrI-B. Podgrupe 16SrI-F i I-P se razlikuju od ostalih podgrupa i na nivou groEL gena kao i na nivou 16S rDNK. TakoĎe je utvrĎeno da sedam novodetektovanih sojeva Aster yellows fitoplazmi iz Srbije pripada podgrupama groELI-III, I-VII i I-IX. Analizom tuf gena, dela rp operona i secY gena kod 22 odabrana od 34 testirana soja soja Aster yellows fitoplazmi moguće je testirane sojeve klasifikovati u šest tufI i sedam rpI i secYI podgrupa na osnovu MboI, Tsp509I i Tru1I restrikcionih profila za tuf gen, HhaI, AluI i Tsp509I za rp gen i Tsp509I i Tru1I za secY gen. Podgrupa 16SrI-A je dalje diferencirana u dve tufI podgrupe, odnosno 16SrI-A i I-B u po dve rpI i secYI podgrupe, dok podgrupe 16SrI-B, I-M i I-L nisu pokazale nikakve meĎusobne razlike. Podgrupe 16SrI-C, I-F i I-P se razlikuju od ostalih podgrupa i na nivou sva tri testirana gena kao i na nivou 16S rDNK" ]

[ "The plant samples with symptoms typical for phytoplasma infection were collected in Serbia from 2009 till 2011. In those samples phytoplasmas were detected by PCR technique with universal primers P1/P7 and R16F2n/R16R2 and identified according to their Tru1I, HhaI, RsaI and Tsp509I restriction profiles of digested R16F2n/R16R2 amplicons as Aster yellows phytoplasma (ribosomal group 16SrI), subgroups 16SrI-B, I-C and I-P or as Stolbur phytoplasma (ribosomal group 16SrXII). Applying the same PCR-RFLP method on 19 strains of Aster yellows phytoplasmas from collection and three strains from carrot from Serbia, it was confirmed that this strains belong to subgroups 16SrI-A, I-B, I-C and I-F as stated in literature. Also the subgroups were determined for four strains from collection that are used in analyses for the first time and that have no literature data. Using newly designed primers AYgroesF/AYampR and AYgroelF/AYgroelR, the groEL gene was successfully amplified in all 34 strains of Aster yellows phytoplasma tested. RFLP analyses of AYgroelF/AYgroelR amplicons with Tru1I i AluI restriction enzymes revealed existence of six and eight different restriction profiles, respectively, according to which all tested strains were classified in nine groELI subgroups (groELI-I till groELI-IX). On the basis of groEL gene, subgroups 16SrI-A and I-C were further differentiated into two groELI subgroups, 16SrI-B into three, while subgroups 16SrI-M and I-L showed no difference to some strains belonging to subgroup 16SrI-B. Subgroups 16SrI-F and I-P could be differentiated from other subgroups on the basis of groEL gene as on the basis of 16S rDNA. The seven newly detected Aster yellows strains from Serbia were affiliated to subgroups groELI-III, I-VII and I-IX. RFLP analyses with MboI, Tsp509I and Tru1I restriction enzymes of tuf gene, HhaI, AluI and Tsp509I of rp gene and Tsp509I and Tru1I of secY gene, classified 22 selected Aster yellows strains into six tufI subgroups and seven rpI and secYI subgroups. Subgroup 16SrI-A was further differentiated into two tufI subgroups and subgroups 16SrI-A and I-B were further differentiated into two rpI and secYI subgroups each. On the other hand subgroups 16SrI-B, I-M and I-L showed no mutual differences, while subgroups 16SrI-C, I-F and I-P could be differentiated from other subgroups on the basis of all three genes tested as on the basis of 16S rDNA. RFLP analysis of tuf gen with HpaII restriction enzyme showed that all 116 tested Stolbur phytoplasma strains from Serbia belong to tuf type II variant. For further analyses, 39 Stolbur strains were selected out of 116 detected and two strains from grapevine from Croatia were also included in analyses as tuf type I reference strains to gain a larger picture of variability of groEL gene in Stolbur phytoplasmas." ]

[ "info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31043/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "varijabilnost gena", "RFLP", "groELI podgrupe", "tufI podgrupe", "rpI podgrupe", "secYI podgrupe", "Stolbur fitoplazme", "filogenetska analiza" ]

[ "gene variability", "RFLP", "groELI subgroups", "tufI subgroups", "rpI subgroups", "secYI subgroups", "Stolbur phytoplasma", "phylogenetic analysis" ]

[ "Gen za veliku subjedinicu šaperonina (groEL gen) kao dodatni molekularni marker za diferencijaciju fitoplazmi srodnih 'Candidatus Phytoplasma asteris'", "Molecular chaperonin large subunit gene (gro EL gene) as an additional marker for differntiation of 'Canidatus Phytoplasma asteris' - related strains" ]

[ "" ]

[ "BY-NC-ND" ]

[ "http://nardus.mpn.gov.rs/bitstream/id/2289/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/2289/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2080" ]

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[ "" ]

[ "Čolić, Miodrag" ]

[ "Božić, Biljana", "Vučević, Dragana" ]

[ "2016-01-05T11:45:24", "2020-07-03T08:10:43" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/2024", "http://eteze.bg.ac.rs/application/showtheses?thesesId=8", "https://fedorabg.bg.ac.rs/fedora/get/o:2129/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=" ]

[ "Dendritske ćelije (DĆ), najpotentnije antigen-prezentujuće ćelije (APĆ), integrišu signale koje primaju sa različitih receptora u jedinstveni odgovor. Ključni značaj u ostvarivanju funkcija DĆ imaju receptori za prepoznavanje konzervisanih struktura, tzv. molekularnih obrazaca patogena (engl. Pattern Recognition Receptor, PRR). Aktivacija pojedinačnih PRR, posebno Toll-sličnih receptora (engl. Toll-like receptor, TLR) ili lektinskih receptora C-tipa poput dektina-1, dovodi do sazrevanja DĆ, dok je za razvoj efikasnog imunskog odgovora neophodna kooperacija više receptora. Dektin-1 receptor je, pored TLR, jedini receptor urođenog imuniteta čija aktivacija samostalno indukuje signalnu kaskadu koja dovodi do sazrevanja MoDĆ sa sposobnošću indukcije Th1 i Th17 odgovora. Imajući navedno u vidu, sledeći cilj ovog istraživanja je bio da se ispita efekat kombinovane primene Poly (I:C) i kurdlana, agonista dektin-1 receptora, na funkcionalne i fenotipske karakteristike MoDĆ. Za modulaciju sazrevanja i funkcija DĆ, pored stimulacije PRR, značajnu ulogu imaju i citokini prirodnog imuniteta koji se produkuju prilikom infekcije. Najnovija istraživanja ukazuju na ulogu TNF-α, jednog od najznačajnijih proinflamatornih citokina, u sazrevanju DĆ u ranim fazama infekcije kao i razvoju antigen-specifičnog odgovora. Naredni cilj našeg istraživanja bio je ispitivanje dozno- i vremenski- zavisnog efekta kombinovane primene TNF-α i Poly (I:C) na funkcionalne i fenotipske karakteristike MoDĆ. Poslednji deo istraživanja se odnosio na ispitivanje uticaja signala stečenog imuniteta koje DĆ dobijaju tokom interakcije sa T-limfocitima, uključujući signalizaciju preko CD40 receptora i receptora za IFN-γ na njihove karakteristike. Metode. Nezrele MoDĆ, dobijene kultivacijom humanih monocita, su stimulisane samim Poly (I:C) ili njegovom kombinacijom sa loksoribinom, kurdlanom ili TNF-α tokom 48h. Da bi ispitali uticaj signala stečenog imuniteta, MoDĆ stimulisane samim Poly (I:C) ili u kombinaciji sa TNF-α dalje su kultivisane u prisustvu ćelija J558 transfektovanim ligandom za CD40, solubilnog CD40L ili IFN-γ. U cilju ispitivanja uticaja kinetike aktivacije na kapacitet MoDĆ da polarizuju imunski odgovor, MoDĆ su stimulisane kombinacijama različitih koncentracija Poly (I:C) i TNF-α tokom 24h i 48h. Protočnom citofluorimetrijom su analizirane fenotipske karakteristike MoDĆ. Alostimulatorna sposobnost MoDĆ je određena testom mešane leukocitne reakcije. Produkcija citokina je određena ELISA metodom i testom za detekciju citokina pomoću imunofluorescentnih kuglica. Rezultati. Stimulacija MoDĆ optimalnom koncentracijom Poly (I:C) dovela je do povećanja ekspresije HLA-DR, CD86, CD40, CD54, CD83 i CCR7 molekula, povećanja produkcije IL-12, umerene produkcije IL-23 i niske produkcije IL-10. Ovako stimulisane MoDĆ dovode do povećane produkcije IFN-γ i umerene produkcije IL-17 tokom kokultivacije sa CD4+ T limfocitima. U preliminarnim eksperimentima nezrele MoDĆ su stimulisane različitim koncentracijama Poly (I:C) (5 μg/ml, 10 μg/ml, 25 μg/ml i 50 μg/ml), loksoribina (34 μg/ml i 85 μg/ml) i kurdlana (10 μg/ml , 50 μg/ml, 100 μg/ml i 200 μg/ml). Na osnovu fenotipskih i funkcionalnih karakteristika MoDĆ procenjeno je da je optimalna koncentracija za aktivaciju MoDĆ za Poly (I:C) 25 μg/ml, za loksoribin 85 μg/ml i za kurdlan 100 μg/ml, dok je koncentracija Poly (I:C) od 10 μg/ml i loksoribina od 34 μg/ml suboptimalna. Optimalne i suboptimalne koncentracije agonista su dalje korišćene za stimulaciju nezrelih MoDĆ u ovom istraživanju." ]

[ "Dendritic cells (DCs) are the most potent antigen presenting cells (APCs) which receive and integrate multiple signals to initiate and direct a response appropriate to extracellular milleu. These APCs perform these functions mostly due to the expression of a wide variety of pattern recognition receptors (PRRs). PRRs discriminate self-tissues from infectious non-self tissues through molecular pattern (MPs) recognition. Although triggering of a single pattern recognition receptor (PRR), especially Toll-like receptors (TLRs) or C-type lectins, results in phenotypic changes in DCs, for functional maturation cooperativity between multiple PRRs is needed in order to achieve an effective immune response. Recent studies have shown that the ligation of Dectin-1, C-type lectin receptor, on MoDCs elicits their maturation. Dectin-1, a DC-associated C-type lectin, is the first of many PRRs which mediate their own signaling and induces the maturation of DCs capable of eliciting the generation of different T helper (Th) effectors. The next aim of this work was to study the response of MoDCs to the combined effect of Poly (I:C) and curdlan, selective Dectin-1 agonists. Tumor necrosis factor (TNF)-α is important for early DC maturation and as a bridge between initiation of the inflammatory cascade and generation of the antigen-specific response. To gain insight into this scientific problem we investigated the kinetics of maturation and the length of exposure of MoDCs to a pathogen (mimicked by Poly (I:C) in our study) in an inflamed tissue (mimicked by TNF-α) on phenotypic and functional characteristics of MoDCs. Finally, little is known about how subsequent interaction of MoDCs with T cell-derived stimuli, such as CD40 or interferon-γ (IFN-γ), modulates MoDC functions. Therefore, this problem was the last objective of this study. Methods. Immature MoDCs (iMoDCs), generated from human monocytes, were treated with Poly (I:C) alone or in combination with loxoribine, curdlan or TNF-α for 48h. To investigate the influence of T-cell derived stimuli, MoDCs cultivated for 24h with Poly (I:C) alone or in combination with TNF-α were incubated either with CD40 ligand (L)-transfected J558 cells, soluble CD40L or IFN-γ for additional 24h. To examine the influence of kinetics of activation on the Th polarizing capability of MoDCs, we stimulated MoDCs with different doses of Poly (I:C) in combination with TNF-α for 24h and 48h. Phenotypic characteristics of MoDCs were determined by flow cytometry. Allostimulatory capability of MoDCs was tested using a mixed leukocyte reaction assay. Cytokine production was measured by ELISA and FlowCytomix. Results. Optimal concentration of Poly (I:C) stimulated the maturation of MoDCs as judged by the up-regulation of HLA-DR, CD86, CD40, CD54, CD83 and CCR7 expression. Poly (I:C)-treated MoDCs were potent producers of interleukin (IL)-12, moderate producers of IL-23 and weak producers of IL-10, which was followed by high production of IFN-γ and moderate production of IL-17 by allogeneic CD4+ T cells. In preliminary experiments iMoDCs were treated with Poly (I:C) (5 μg/ml, 10 μg/ml, 25 μg/ml and 50 μg/ml), loxoribine (34 μg/ml and 85 μg/ml) or curdlan (10 μg/ml , 50 μg/ml, 100 μg/ml and 200 μg/ml). Based on phenotypic characteristics and functional capabilities of MoDCs, the concentrations of Poly (I:C) (25 μg/ml), loxoribine (85 μg/ml) and curdlan (100 μg/ml) were found to be optimal for activation of MoDCs, while the concentrations of Poly (I:C) (10 μg/ml) and loxoribine (34 μg/ml) were found to be suboptimal. For stimulation of iMoDCs we used these concentrations of the agonists. The combined treatment of MoDCs with suboptimal concentrations of TLR3 and TLR7 agonists resulted in slight potentiation of HLA-DR, CD86, CD83, CD54 and CD40 molecules and stimulation of IL-27, IL-23 and IL-10 secretion, compared to effects of single agonists. This was followed by up-regulated secretion of IFN-γ and IL-17 in the co-culture with allogeneic CD4+ T cells. When the suboptimal concentration of Poly (I:C) was combined with the optimal concentration of loxoribine, MoDCs down-regulated HLA-DR and up-regulated CD86 expression, enhanced the production of IL-12 and IL-23 and down-regulated the levels of IL-10 and IL-27, compared to the effects of single agonists. MoDCs pretreated in this way stimulated the production of IFN-γ and lowered the levels of IL-4 and IL-10 by CD4+ T cells. The treatment of MoDCs with optimal concentrations of both TLR agonists was followed by down-regulation of HLA-DR, CD83 and CD40 expression and augmented the production of IL-12, IL-27 and IL-10, whereas the level of IL-23 was significantly lower, compared to relevant controls. These MoDCs promoted the production of IFN-γ and inhibited the production of IL-4, IL-10 and IL-17 in co-culture, compared to the effect of corresponding controls. The combination of Poly (I:C) and curdlan induced phenotypic maturation of MoDCs with the capability to stimulate alloreactive response. Such treated MoDCs up-regulated the production IL-12, IL-23 and IL-10, compared to the effect of Poly (I:C), alone. The opposite effect was observed for IFN-γ production. When combined, these agonists primed MoDCs to further increase the production of IFN-γ by CD4+ T cells in co-culture, especially those of naïve (CD45RA+) phenotype, and IL-17 by memory (CD45RO+) CD4+ T cells." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175102/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "CD40", "CD4+ T-ćelijski odgovor", "Dektin-1 receptor", "humane dendritske ćelije monocitnog porekla", "imunoterapija tumora", "IFN-γ", "TNF-α", "Toll-sličan receptor 3", "Toll-sličan receptor 7" ]

[ "CD40", "Dectin-1 receptor", "human monocyte-derived dendritic cells", "IFN-γ", "TNF-α", "T helper immune response", "Toll-like receptor 3", "Toll-like receptor 7", "tumor immunotherapy" ]

[ "Modulacija funkcije humanih dendritskih ćelija kombinovanom primenom agonista endozomnih Toll-sličnih receptora, Dektin-1 receptora i proinflamatornih citokina", "Modulation of the function of human monocyte derived dentritic cells by combined use of the endosomal Toll-like receptors, Dectin-1receptor and proinflamatory cytokines" ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/2364/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2364/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2024" ]

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[ "Krtolica-Žikić, Koviljka" ]

[ "Brajušković, Goran", "Radojković, Milica" ]

[ "2016-01-05T11:47:45", "2020-07-03T08:09:55" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/2119", "http://eteze.bg.ac.rs/application/showtheses?thesesId=806", "https://fedorabg.bg.ac.rs/fedora/get/o:7138/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024576946" ]

[ "Difuzni B-krupnoćelijski limfom (DBKL) i folikularni limfom (FL), predstavljaju najučestalije entitete nehočkinovih limfoma i zajedno čine oko 50 % svih B-ćelijskih neoplazija. To su klinički i biološki heterogena oboljenja, sa visoko varijabilnim odgovorom na terapiju i uprkos značajnom napretku u terapiji, u velikom broju slučajeva ostaju neizlečiva. Aktuelni terapijski pristupi su praćeni brojnim neželjenim efektima i dovode do dugotrajne remisije i preživljavanja kod svega 50 % bolesnika. Najznačajniji klinički prediktor ishoda bolesti do danas je internacionalni prognostički indeks (IPI za DBKL, odnosno, FLIPI za FL), koji obuhvata odgovarajuće kliničke prognostičke parametre. Uprkos tome, oboleli koji pripadaju istim IPI/FLIPI kategorijama ispoljavaju značajne razlike u odgovoru na terapiju i preživljavanju, što ukazuje na postojanje značajne biološke heterogenosti u okviru svake IPI/FLIPI kategorije. Zbog toga je neophodno pronalaženje novih prognostičkih molekularnih parametara, koji bi omogućili precizniju podelu obolelih u grupe različitog stepena rizika i izbor odgovarajuće terapije. Primenom PCR metode specifične za metilaciju (MSP), ispitivan je metilacioni status p16, p15, MGMT i DAPK gena kod obolelih od DBKL i FL, kao bi se utvrdio njihov potencijalni prognostički značaj. Kod obolelih od DBKL, metilacija nijednog gena nije pokazala prognostički značaj, mada je uočena tendencija ka akumulaciji metilacije p15 gena kod ispitanika sa povoljnijim kliničko-patološkim karakteristikama. Kod obolelih od FL metilacija p16, p15 i MGMT gena bi mogla da ima određeni prognostički značaj, ukoliko se kombinuje sa FLIPI, gradusom tumora, odnosno starosnim dobom obolelihi. Istovremena metilacija MGMT i DAPK gena bi mogla da ukaže na grupu obolelih potencijalno hemorezistentnih na primenjenu hemoterapiju i sklonu recidivima bolesti." ]

[ "Diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma (FL) are the most comon subgroups of non-Hodgkin’s lymphoma and comprise approximately 50 % of all cases. They represents clinically and biologically heterogeneous diseases, characterized with highly variable response to the treatment, and remain incurable despite the significant advances in therapy. Actual therapy regimens are agressive, and long-term remission and survival are ashieved in only 50 % of patients. The strongest clinical predictor of outcome to date in DLBCL and FL patients is the International Prognostic Index (IPI for DLBCL and FLIPI for FL) which includes several clinico-pathological prognostic parameters. However, patients with identical IPI/FLIPI still exhibit marked variability in survival, suggesting the presence of significant biological heterogenity within the same risk category. So, it is important to investigate additional molecular markers in order to further stratify patients into diferent risk groups, choose appropriate treatement strategy and improve prognosis. In this study, methylation-specific polymerase chain reaction (MSP) was used to investigate the role and potential prognostic significance of the methylation status of p16, p15, MGMT and DAPK genes in patients with DLBCL and FL. No one of four examined genes showed prognostic significance in patients with DLBCL, though we observed a tendency toward accumulation of p15 methylation with favorable clinico-pathological parameters. However, in patients with FL, our results suggest that promoter methylation of p16, p15 and MGMT genes could have some prognostic value when used in combination with the FLIPI, tumor grade and patients age, respectively. Concurrent methylation of MGMT and DAPK genes could be the marker of tumor chemoresistance and disease recurrence." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173049/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "difuzni B-krupnoćelijski limfom (DBKL)", "folikularni limfom (FL)", "metilacija", "p16", "p15", "MGMT", "DAPK" ]

[ "Diffuse large B-cell lymphoma (DLBCL)", "Follicular lymphoma (FL)", "Methylation", "p16", "p15", "MGMT", "DAPK" ]

[ "Prognostički značaj aberantne metilacije CpG ostrvaca u folikularnim i difuznim B-krupnoćelijskim limfomima čoveka", "Prognostic significance of aberrant CpG islands methylation in human follicular and diffuse large B-cell lymphoma" ]

[ "" ]

[ "BY-NC-ND" ]

[ "http://nardus.mpn.gov.rs/bitstream/id/2203/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/2203/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2119" ]

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[ "Stević, Zorica" ]

[ "Lavrnić, Dragana", "Novaković, Ivana", "Raičević, Ranko" ]

[ "2019-02-26T11:22:30", "2020-07-03T08:50:50" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/10774", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6610", "https://fedorabg.bg.ac.rs/fedora/get/o:19410/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50790159" ]

[ "Amiotrofična lateralna skleroza (ALS) nastaje usled primarnog oštećenja motornih neurona na nivou kore velikog mozga, jedara moždanog stabla i prednjih rogova kičmene moždine. Oko 90% ALS pacijenata čini tzv. sporadičnu ALS (SALS), dok 5-10% pacijenta ima genetsku ili familijarnu osnovu bolesti (FALS). Do sada je opisano više od 20 gena povezanih sa ALS. Nisu poznate epidemiološke i kliničke karakteristike pojedinih formi FALS u Srbiji. U svetu nedostaju podaci o zahvaćenosti centralnog nervnog sistema (CNS) u sklopu FALS. Ciljevi ovog istraživanja su bili: molekularno-genetsko ispitivanje pacijenata sa ALS na prostoru Srbije; određivanje tipičnih kliničkih znakova u subpopulaciji pacijenata sa FALS; ispitivanje korelacija kliničkih i genetskih karakteristika, uključujući poređenje nalaza neuropsihološkog testiranja i morfoloških specifičnosti mozga i kičmene moždine analiziranih pomoću savremenih metoda magnetne rezonancije (MR). Metod: U ovo ispitivanje uključen je 241 pacijent iz hospitalnog registra Klinike za neurologiju Kliničkog centra Srbije. Pacijenti su testirani na sledeće mutacije: SOD1 (svih 5 egzona), TDP 43 (egzon 6), FUS (egzoni 14 i 15), c9orf72 i ANG. Genetska grupa od 37 ispitanika poređena je klinički sa 32 SALS pacijenta bez verifikovane mutacije. Neuropsihološko testiranje je sprovedeno kod 22 SOD1 pozitivna ispitanika, 5 SOD1 negativnih ispitanika pozitivnih na druge ALS gene i 82 zdrave kontrole. Primenjivani su testovi za kognitivni skrining, procenu opšteg intelektualnog nivoa, pažnje, pamćenja, vizuospacijalnih i konstrukcionih, egzekutivnih i jezičkih funkcija. Od 69 pacijenta sa ALS, MR pregledom mozga i vratnog dela kičmene moždine na aparatu od 1,5T je obuhvaćena grupa od 23 genetski determinisanih pacijenata i 11 iz grupe SALS. Kontrolna grupa bila je sastavljena od 34 uparena zdrava ispitanika. Analizirana je debljina korteksa, mikrostruktura puteva bele mase, funkcionalna povezanost delova mozga u stanju mirovanja, kao i analiza strukture kičmene moždine. Rezultati: Ukupno 37 (15,4%) od 241 pacijenta imalo je jednu ili više mutacija u testiranim ALS genima. U genetskoj grupi 27 pacijenta je imalo mutaciju u SOD1 genu (72,9%), šest pacijenata je imalo mutaciju u genu za c9orf72 (16,2 %), dva pacijenta mutaciju u ANG genu (5.4%), jedan pacijent je imao mutaciju u TDP43 genu (2,7%), a jedan kombinovanu mutaciju c9orf72, FUS i ANG (2,7%). Svi SOD1 pozitivni pacijenti imali su spinalni početak bolesti i u većini slučajeva (85,2%) prvi simptomi su bili u donjim udovima..." ]

[ "Amyotrophic lateral sclerosis (ALS) is caused by damage of motor neurons in bran cortex, brainstem and the spinal cord. About 90% of ALS patients have sporadic disease form (SALS), while 5-10% of patients have a genetic or familial disease (FALS). So far, more than 20 genes have been associated with ALS. The epidemiological and clinical characteristics of certain forms of FALS in Serbia are not known. Furthermore, there is a lack of data on central nervous system (CNS) involvement in FALS worldwide, both in terms of neuroimaging and cognitive testing. The objectives of this research were: molecular-genetic testing of ALS patients in Serbia; determination of typical clinical signs in subpopulation of patients with FALS; correlations of clinical and genetic characteristics, including neuropsychological testing and morphological specificities of the brain and spinal cord analyzed using modern magnetic resonance imaging (MRI) methods. Method: This study included 241 patients from the Neurology Clinic, Clinical Center of Serbia. Patients were tested for the following mutations: SOD1 (all 5 exons), TDP 43 (exon 6), FUS (exon 14 and 15), c9orf72 and ANG. A genetic group of 37 subjects was compared clinically with 32 SALS patients without a verified mutation. Neuropsychological testing was performed in 22 SOD1 positive subjects, 5 SOD1 negative subjects positive for other ALS genes and 82 healthy controls (HCs). Cognitive screening, as well as tests of general intellectual level, attention, memory, visuospacial, executive and linguistic functions were applied. Of the 69 patients with ALS, MRI examination of the brain and cervical spinal cord on the 1.5T equipment were conducted on 23 genetically determined patients and 11 SALS subjects. The control group was composed of 34 subjects macthed for gender and age. Cortical thickness analysis, white matter microstructural assessment, functional MRI at resting state, and the analysis of the spinal cord were made. Results: A total of 37 (15.4%) of 241 patients had one or more mutations in tested ALS gene. In the genetic group, 27 patients had a mutation in the SOD1 gene (72.9%), six patients had a mutation in the c9orf72 gene (16.2%), two in the ANG gene (5.4%), one in the TDP43 gene (2.7%), and one had combined mutations of c9orf72, FUS and ANG (2.7%). All SOD1 positive patients had the spinal onset of the disease, and in most cases (85.2%), the first symptoms were in the lower limbs..." ]

[ "" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nd/4.0/" ]

[ "amiotrofična lateralna skleroza (ALS)", "SOD1 gen", "c9orf72", "kognicija", "difuzioni tenzorski imidžing", "debljina korteksa", "funkcionalna povezanost mozga", "atrofija kičme moždine" ]

[ "lateral sclerosis (ALS)", "SOD1 gene", "c9orf72", "cognition", "diffusion tensor imaging", "cortical thickness", "functional brain MRI", "spinal cord atrophy" ]

[ "Kliničko genetska analiza pacijenata sa familijarnom amiotrofičkom lateralnom sklerozom u populaciji Srbije" ]

[ "Clinical and genetic analysis of patients with familiar amyotrophic lateral sclerosis in Serbian population" ]

[ "BY-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/10030/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/10031/IzvestajKomisije19087.pdf", "http://nardus.mpn.gov.rs/bitstream/id/10030/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/10031/IzvestajKomisije19087.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_10774" ]

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[ "" ]

[ "Vićovac-Panić, Ljiljana" ]

[ "Đorđević, Jelena", "Jovanović-Krivokuća, Milica", "Vasilijić, Saša" ]

[ "2016-01-05T11:48:04", "2020-07-03T08:10:15" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/2139", "http://eteze.bg.ac.rs/application/showtheses?thesesId=1070", "https://fedorabg.bg.ac.rs/fedora/get/o:7771/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=44889103" ]

[ "Prolaktin (PRL) je polipeptidni hormon koji utiče na rast i diferencijaciju različitih ćelija, a uključen je i u brojne fiziološke procese. Sintetiše ga i luči hipofiza, ali i različita druga tkiva i ćelije. Prolaktin je jedan od glavnih proteina koji sintetiše i sekretuje decidualizovani endometrijum. Produkcija decidualnog prolaktina se povećava u trudnoći nakon implantacije, i dostiže maksimum u tkivu decidue 20 do 25 nedelje gestacije. Delovanje PRL se ostvaruje vezivanjem za PRL receptore (PRLR) na ćelijskoj membrani. Poznato je da se za prolaktinski receptor vezuje najmanje tri liganda - prolaktin, placentni laktogen i hormon rasta. Do sada je za placentu poznato da je receptor za prolaktin ispoljen u decidui, trofoblastu horiona, epitelu amniona i sinciciotrofoblastu na kraju trudnoće. Prolaktin u različitim ćelijama i tkivima utiče na ekspresiju adhezionih i proteolitičkih molekula koji su značajni za degradaciju ekstraćelijskog matriksa i ćelijsku migraciju. Međutim, njegova uloga u trofoblastu nije dovoljno poznata. Ovim radom je po prvi put ispitivan uticaj prolaktina na svojstva invazivnog ekstravilusnog trofoblasta prvog trimestra trudnoće. Detektovana je ekspresija PRLR, kao i profil prisutnih formi receptora na izolovanim citotrofoblastnim ćelijama i HTR-8/SVneo trofoblastnoj imortalizovanoj ćelijskoj liniji. Pokazali smo da PRL stimuliše adheziju, migraciju i invaziju trofoblasta in vitro. Kao potencijalne efektore ispitivali smo integrine, gal-1 i matriksne metaloproteinaze-2 i -9. Dobijeni rezultati ukazuju da PRL stimuliše ekspresiju subjedinica integrina α1, α5, kao i gal-1. Ispitivali smo i efekat PRL na vijabilnost, proliferaciju i apoptozu HTR-8/SVneo ćelija. PRL je blago stimulisao vijabilnost i uticao na povećanje broja adherentnih ćelija, dok apoptoza nije bila značajno promenjena. Dobijeni rezultati predstavljaju napredak u razumevanju fiziološke uloge PRL značajne za funkciju humanog trofoblasta. Takođe, ovaj rad pruža celovitiji uvid u proces diferencijacije ranog trofoblasta i predstavlja dobru osnovu za dalja ispitivanja biološke uloge prolaktina, kao i drugih članova ove familije proteina u humanom trofoblastu." ]

[ "Prolactin (PRL) is a polypeptide hormone which has impact on the growth and differentiation of various cell types, and is known to participate in numerous physiological processes. It is synthesized and secreted by the pituitary gland and many other tissues and cell types. Prolactin is also one of the major proteins synthesized and secreted by decidualized endometrium. The production of decidual prolactin increases after implantation, and peaks in decidual tissue at 20 to 25th week of gestation. The action of PRL is exerted through binding to its receptors (PRLR) at the surface of target cells. It is known that prolactin receptor has at least three ligands – prolactin, placental lactogen and growth hormone. Regarding localization of prolactin receptor in placenta, so far it has been found in decidua, chorionic trophoblast, amniotic epithelium and synciciotrophoblast at term. Prolactin affects the expression of adhesion and proteolytic molecules which are important for degradation of extracellular matrix and cell migration. However, its role in trophoblast is not well defined. In this study the effect of prolactin on the function of first trimester of pregnancy extravillous trophoblast was studied for the first time. The expression of PRLR, as well as the profile of different isoforms was examined in both cytotrophoblast and HTR-8/SVneo trophoblast derived cell line. It is shown here that PRL stimulates trophoblast cell adhesion, migration and invasion in vitro. Potential effectors were sought among integrins, gal-1 and matrix metalloproteinases-2 and -9. The results showed that PRL stimulated the expression of integrin subunits α1, α5, as well as gal-1. When investigating the effect of PRL on cell viability, proliferation and apoptosis of HTR-8/SVneo cells, PRL was found to slightly stimulate cell viability and adherent cell number, while apoptosis was not altered. The results of this study represent a step further in the understanding of the physiological role of PRL in human trophoblast. Moreover, this work gives a better insight in to the process of early trophoblast differentiation and represents a good basis for further studies of the biological role of prolactin, as well as other members of this family of proteins in the human trophoblast." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173004/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "prolaktin", "PRLR", "trofoblast", "izolovani citotrofoblast", "HTR-8/SVneo." ]

[ "prolactin", "PRLR", "trophoblast", "isolated cytotrophoblast", "HTR-8/SVneo" ]

[ "Uticaj prolaktina na ćelije trofoblasta na čoveka in vitro", "The effect of prolactin on human trophoblast in vitro" ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/2270/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2270/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2139" ]

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[ "Latas, Milan" ]

[ "Ristić-Medić, Danijela", "Pejović-Milovančević, Milica", "Ivković, Maja", "Vasiljević, Nađa" ]

[ "2018-12-19T16:02:43", "2020-07-03T08:45:50" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/10350", "http://eteze.bg.ac.rs/application/showtheses?thesesId=6352", "https://fedorabg.bg.ac.rs/fedora/get/o:19034/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50779407" ]

[ "Gojaznost je hronično oboljenje sa značajnim nepovoljnim efektima na zdravlje, a broj gojaznih osoba u svetu dostigao je epidemijske razmere. Medicinska nutritivna terapija predstavlja terapiju izbora kod lečenja gojaznosti, ali je njena uspešnost ograničena. Praćena je značajnim procentom odustajanja kao i ponovnim dobijanjem na težini. Cilj ove studije je bio da se proceni da li psihijatrijski i specifični psihološki faktori mogu da utiču na uspešnost odnosno neuspešnost terapije. Metode: Studijom su obuhvaćene ukupno 84 žene predgojazne i gojazne I stepena starosne dobi 20 do 40 godina, koje su bile motivisane za medicinsku nutritivnu terapiju. Sve ispitanice su prošle individualno savetovanje i dobile dnevni nutritivno balansiran plan ishrane koji je podrazumevao kalorijsku restikciju od 300-500kcal u odnosu na procenjene dnevne energetske potrebe. Na početku studije primenjeni su upitnici: Lista simptoma od 90 pitanja (90-item Symptom Check-list) i Baratova skala impulsivnosti (Barratt Impulsiveness scale - BIS 11), Skala opštih stavova i verovanja (The General Attitude and Belief Scale - GABS 55), Upitnik frustracione netolerancije (The Frustration Discomfort Scale - FDS) kao i za ovu studiju dizajniran socio-demografski upitnik. Nakon 6 meseci primene kalorijski restriktivnog načina ishrane ponovljena su antropometrijska merenja. Ispitanice su podeljene u dve grupe: 40 ispitanica (48 %) koje su izgubile ≥ 10% od početne telesne mase (Uspešne) i druga od 44 ispitanice (52 %) koje su izgubile < 10% od početne telesne mase (Neuspešne). Rezultati. Nije bilo značajne razlike između grupa ispitanica u odnosu na sociodemografske karakteristike (godine života, stepen obrazovanja, zaposlenost, bračni status, navika pušenja, uzimanje alkohola), antropometrijske parametre na početku studije kao i u skoru opšte psihopatologije i dimenzija simptoma..." ]

[ "Obesity is a chronic disease and health threatening condition. The numbers of obese people in the world have taken epidemic proportions. Medical nutritional therapy is the first choice in the treatment of obesity but it is also accompanied with a great percentage of attrition and a significant weight regain. The aim of our study was to evaluate if psychiatric and specific psychological factors could be the predictors of successful weight loss. Method. Study sample consisted of eighty four consecutive overweight/obese women, 20 to 40 years old, who were willing to adhere to medical nutrition therapy. All participants received a personalized nutritional counseling and daily balanced diet treatment with treatment with 300-500 caloric restriction from estimated daily energy requirement. At the beginning of the study 90-item Symptom Check-list (SCL-90), 30-item Barratt Impulsiveness scale (BIS-11), The General Attitude and Belief Scale (GABS 55), The Frustration Discomfort Scale - FDS as well as the questionnaire with socio-demographic data designed for this study were administrated. At the end of six months of caloric restriction conventional diet therapy and control weight measurement, patients were divided into two groups: 40 participants (48 %) who lost ≥10% of their initial body weight (Successful) and 44 participants (52 %) who lost <10% of their initial weight (Unsuccessful). Results. There were no significant differences between both groups in demographic data (age, level of education, employment, marital status, smoking, alcohol consumption), baseline anthropometric parameters and general psychopathology as well as symptom dimensions. Successful group had higher levels of pshisical activity..." ]

[ "" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "žene", "gojaznost", "medicinska nutritivna terapija", "opšta psihopatologija", "impulsivnost", "tolerancija na frustraciju", "racionalna uverenja", "iracionalna uverenja" ]

[ "women", "obesity", "medical nutrition therapy", "general psychopathology", "impulsivity", "frustration intolerance", "rationale beleifs irrational beleifs" ]

[ "Identifikacija psihijatrijskih i psiholoških prediktora efikasnosti medicinske nutritivne terapije gojaznosti" ]

[ "Identification of psychiatric and psychological predictors of medical nutrition therapy for obesty outcome" ]

[ "BY-NC-ND" ]

[ "http://nardus.mpn.gov.rs/bitstream/id/8127/IzvestajKomisije18527.pdf", "https://nardus.mpn.gov.rs/bitstream/id/8126/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/8127/IzvestajKomisije18527.pdf", "http://nardus.mpn.gov.rs/bitstream/id/8126/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_10350" ]

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[ "Kanazir, Selma" ]

[ "Korać, Bato", "Mladenović-Đorđević, Aleksandra", "Ruždijić, Sabera", "Rakić, Ljubisav" ]

[ "2016-01-05T11:45:18", "2020-07-03T08:10:33" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/2017", "http://eteze.bg.ac.rs/application/showtheses?thesesId=27", "https://fedorabg.bg.ac.rs/fedora/get/o:2272/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=41541647" ]

[ "Starenje centralnog nervnog sistema (CNS) praćeno je brojnim promenama koje dovode do narušavanja nervnih funkcija, pre svega kontrole motorike i kognitivnih sposobnosti. Smatra se da su kognitivni poremećaji tokom starenja posledica smanjene sinaptičke plastičnosti, naročito u korteksu i hipokampusu, regionima mozga izuzetno važnim za procese učenja i pamćenja. Najvažniji faktor sredine kojim se može uticati na starenje uopšte, a samim tim i na starenje mozga je režim redukovane ishrane, bez pothranjenosti. Pored toga što se već nekoliko decenija zna da redukovana ishrana produžava životni vek mnogih vrsta, uključujući i sisare, pokazano je da ona odlaže i ublažava starosno zavisne promene u nervnom sistemu. Holesterol je najzastupljeniji lipid u ćelijama sisara. Učestvujući u izgradnji fosfolipidnog dvosloja bioloških mambrana, on reguliše njihovu fluidnost, propustljivost i rigidnost, a samim tim i funkcionalna svojstva membranskih proteina, poput jonskih kanala i transmiterskih receptora. Metabolizam holesterola predstavlja važan aspekt sinaptičke plastičnosti, jer je pokazano da je holesterol neophodan za biogenezu i transport sinaptičkih vezikula, kao i za prvilnu funkcionalnu organizaciju lipidnih ostvaca. Od svih organa u telu, mozak sadrži najviše holesterola. Održavanje homeostaze holesterola neophodno je za normalno i neometano funkcionisanje CNS..." ]

[ "Ageing of the central nervous system is associated with a number of changes that disturb nerve function, especially motor control and cognitive abilities. It is believed that cognitive disorders in aging are consequence of reduced synaptic plasticity, especially in the cortex and hippocampus, brain regions extremely important for learning and memory processes. Dominant factor that can influence aging in general, and therefore the aging of the brain is reduced diet regime, without malnutrition. In addition to being known for decades that reduced caloric intake prolongs life span of many species, including mammals, it is shown that it delays and alleviates age-dependent changes. Cholesterol is the most abundant lipid in mammalian cells. By participating in the construction of phospholipid bilayer of biological membranes, it regulates their fluidity, permeability and rigidity, and consequently the functional properties of membrane proteins, such as ion channels and transmmitters receptors. Cholesterol metabolism represents one aspect of synaptic plasticity, as has been shown that cholesterol is essential for biogenesis of synaptic vesicles, and vesicle transport, as well as for proper functional organization of lipid rafts. The brain contains the most cholesterol of all the organs in the body. Maintenance of cholesterol homeostasis is essential for proper functioning of the central nervous system..." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173056/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "Starenje", "dijetalna restrikcija", "korteks", "hipokampus", "pacov", "holesterol" ]

[ "Ageing", "dietary restriction", "cortex", "hippocampus", "rat", "cholesterol" ]

[ "Uticaj starenja i dugotrajne dijetalne restrikcije na metabolizam holesterola u prednjem mozgu pacova", "Effects of aging and long-term dietary restrictions on cholesterol metabolism in the rat forebrain" ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/2336/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2336/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2017" ]

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[ "Matić, Gordana" ]

[ "Konjević, Gordana", "Tanić, Nikola", "Kataranovski, Milena" ]

[ "2016-01-05T11:47:57", "2020-07-03T08:10:07" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/2130", "http://eteze.bg.ac.rs/application/showtheses?thesesId=919", "https://fedorabg.bg.ac.rs/fedora/get/o:7447/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024630194" ]

[ "Melanom, maligni tumor melanocita, iako ima brojna imunogenicna svojstva, predstavlja tumor kože sa najvišom incidencom smrtnosti. Za anititumorsku imunost u melanomu posebno su znacajne celije prirodne ubice (NK celije), efektorske celije sistema uroene imunosti koje mogu neposredno da prepoznaju maligno transformisane celije i da ih liziraju citolitickim enzimima (perforin i granzimi) kao i da produkcijom citokina i hemokina regulišu adaptivnu i uroenu amtitumorsku imunost. NK celije su prisutne u mnogim tkivima i organima. Kod melanoma su uglavnom proucavane NK celije u perifernj krvi i u tumorskom tkivu, dok NK celije regionalnih limfnih cvorova (LC) obolelih nisu do sada ispitivane. NK celije su CD3-CD56+ i obuhvataju dve funkcionalno razlicite subpopulacije, imunoregulatornu, CD3-CD56sjajno+ i citotoksicnu, CD3-CD56potmulo+. Funkcija NK celija je regulisna balansom signala koji poticu od aktivacionih i inhibitornih NKcelijskih receptora. Aktivacioni receptori prepoznaju stresogene ligande na tumorskim celijama, dok inhibitorni ubilacki receptori slicni imunoglobulinima (engl. Immunoglobulin-like Killer Receptors, KIR) vezivanjem za MHC molekule klase I inhibiraju NK celijsku funkciju i pored toga omogucavaju toleranciju NK celija na celije sopstvenog organizma. NK celije su prisutne u T celijskoj zoni LC, gde se odvija njihovo sazrevanje i diferencijacija. U fiziološkim uslovima u LC perifernom cirkulacijom dospeva uglavnom nezrela (CD3-CD56sjajno+) NK celijska subpopulacija koja cini veci deo NK celijske populacije u mirujucim limfnim cvorovima. Pod uticajem endogenih citokina u LC ova subpopulacija sazreva u citotoksicnu CD3-CD56potmulo+ perforin+CD16+KIR+ subpopulaciju koja zatim napušta LC. U patološkim stanjima dolazi do povecane zastupljenosti CD16+KIR+ NK celija u reaktivnim LC što može biti posledica povecane produkcije citokina uroene imunosti od strane dendriticnih celija, kao i Th1 citokina od strane stimulisanih T celija antigenima u LC, kao i selektivne migracije CD16+KIR+ NK celija u LC..." ]

[ "Introduction: Melanoma, malignant neoplasm of melanocytes, despite of its immunogenicity is skin cancer with the highest mortality rate. In antitumor immunity im melanoma, natural killer (NK) cells as innate immune system effector cells play an important role as they are able to recognize malignantly transformed cells, lyse them by cytolitic enzymes (perforin and gramzymes) and also regulate adaptive and innate antitumor immunity by production of cytokines and chemokines. NK cells are present in many tissues and organs. In melanoma NK cells have been studied mostly in peripheral blood and in tumor tissue, while NK cells in regional lymph nodes (LN)s have not been investigated. NK cells are CD3-CD56+ and comprise two functionally distinct subsets, immunoregulatory, CD3-CD56bright+ and cytotoxic, CD3-CD56dim+. NK cell function is regulated by the balance of signals mediated by activating and inhibitory NK cell receptors. Activating receptors recognize stress induced ligands on tumor cells, while the inhibitory immunoglobulin-like killer receptors (KIR) by binding to MHC class I molecules inhibit NK cell function and enable self tolerance. NK cells are located mostly in T cell zone of LNs, where NK cell differentiation and maturation may occur. In physiological conditions, by peripheral circulation mostly immature CD3-CD56bright+ NK cell subset migrates to LNs and represents the majority of NK cell population in resting LNs. Endogenous cytokines in LN mediate maturation of this subset into cytotoxic CD3-CD56dim+ perforin+CD16+KIR+ subset that subsequently leaves LN. In pathological conditions, increase in CD16+KIR+ NK cell population in reactive LNs may be the consequence of increased production of innate immunity cytokines by dendritic cells, as well as Th1 cytokine production by antigen stimulated T cells in LNs, or selective migration of CD16+KIR+ NK cells into LNs..." ]

[ "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41031/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]

[ "NK celije", "limfni cvorovi", "melanom", "citotoksicnost", "perforin", "NKG2D", "CD16", "KIR", "IL-2", "IL-15" ]

[ "NK cell", "lymph nodes", "melanoma", "cytotoxicity", "perforin", "NKG2D", "CD16", "KIR", "IL-2", "IL-15" ]

[ "Funkcionalne i imunofenotipske karakteristike NK ćelija regionalnih limfnih čvorova obolelih od melanoma i njihova in vitro aktivacija IL-2 i IL-15", "Functional and immunophenotypic characteristics of NK cells from regional lymph nodes of melanoma patients and their in vitro activation with IL-2 and IL-15" ]

[ "" ]

[ "BY-NC-SA" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/2242/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2242/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2130" ]

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[ "Tomanović, Željko" ]

[ "Ćetković, Aleksandar", "Petrović-Obradović, Olivera" ]

[ "2016-01-05T11:47:29", "2020-07-03T08:10:28" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=743", "https://nardus.mpn.gov.rs/handle/123456789/2105", "https://fedorabg.bg.ac.rs/fedora/get/o:7036/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=44659983" ]

[ "Intenzifikacija poljoprivrede predstavlja jedan od globalnih procesa sa izrazito negativnim efektima na biodiverzitet. Održavanje visokog nivoa biodiverziteta neophodno je za obezbeđivanje ekosistemskih usluga, od kojih su neke, poput biološke kontrole štetočina, od velike važnosti u agroekosistemima. Rezultati sve većeg broja studija ukazuju na to da elementi i karakteristike kompozicije i konfiguracije poljoprivrednih predela imaju ključnu ulogu u očuvanju, odnosno, narušavanju agrobiodiverziteta. Predeono-ekološki pristup u agroekologiji proučava uticaj različitih obrazaca i procesa u savremenoj poljoprivrednoj praksi na mehanizme degradacije biodiverziteta i gubitak funkcionalnosti njegovih komponenti. Posebno je značajno upoznati obrazce i trendove u trofičkim interakcijama koje povezuju intenzivno korišćene agroekosisteme i okolna poluprirodna i prirodna staništa. U ovom istraživanju, analizirani su efekti predeonih karakteristika na diverzitet i interakcije model sistema žitne vaši – parazitoidi na području Pančevačkog rita. Istraživanje je bilo podeljeno u tri dela kako bi se ispitali efekti tri različita aspekta predeone heterogenosti. Cilj prvog dela istraživanja bio je određivanje efekta predeone kompleksnosti izražene preko procenta prirodnih i poluprirodnih staništa (heterogenost tipova staništa) na model sistem. Odabrane su dve jasno diferencirane kategorije predela: 1) kompleksni predeli (>50% poluprirodnih staništa) i 2) jednostavni predeli (<30% poluprirodnih staništa) kako bi se testirala razlika između ovih kategorija. Ukupno je analizirano 50 predeonih sektora u toku četiri godine (2008-2011). Drugi deo istraživanje bio je koncipiran da se odrede efekti predeone kompleksnosti izražene preko veličine pojedinačnih polja (heterogenost konfiguracije poljoprivrednih površina) na model sistem. Ponovo, određene su dve jasno diferencirane kategorije predela: 1) predeli sa pretežno malim poljima (<3 ha) i 2) predeli sa velikim poljima (>20 ha). Analizirano je 24 predeona sektora u toku dve godine (2008-2009). Treći deo bio je fokusiran na određivanje efekata različitog tipa ivice polja na model sistem. Kontrastirana su dva tipa ivice polja: 1) ivica sa poluprirodnom vegetacijom i 2) ivica bez poluprirodne vegetacije..." ]

[ "Agricultural intensification and associated farming practices are among the most significant human impacts on the global environment. Increase in agricultural land-use area and the intensification of crop management are causing numerous environmental problems, including loss of biodiversity and degradation of some key ecosystem services, such as biological control. Numerous studies have emphasized the importance of landscape scale effects in these processes. To determine how agricultural intensification affects agrobiodiversity and accompanied ecological service (biological control), we examined effects of key landscape features on aphid–parasitoid complex in winter wheat agroecosystems in Pančevački rit region. Study was organized in three parts. Aim of the first part was to determine effect of landscape compositional heterogeneity defined through proportion of crop vs. non-crop land in landscape sectors. Two broadly contrasting classes were selected: complex landscapes, characterized by more than 50% of non-crop land cover (forests, fallows, pastures, hedgerows and shrubs), and simple landscapes, with much less than 30% of non-crop land. Total of 50 landscape sectors were sampled in course of four years (2008-2011). In the second part of the study, effects of configurational landscape heterogeneity of crop habitats were tested through contrasting landscape sectors dominated by small fields (field size averaging less than 3 ha) and landscape sectors dominated by large fields (field size averaging more than 20 ha). Total of 24 sectors were sampled in two years (2008-2009). In the third part, aim was to analyze edge effects of field margin types on aphid–parasitoid interactions. Two markedly different margin types were selected: one bordering on irrigation canal with permanent semi-natural shrub vegetation, and the other close to service road without semi-natural vegetation. Total of 12 fields were sampled in two years (2008-2009). Additionally, changes in aphid population growth and parasitism rates between phases were monitored to determine parasitism threshold value for effective biological control. In total, 4 aphid species, 7 parasitoid species and 9 hyperparasitoid species were found..." ]

[ "info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43001/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]

[ "biljne vaši", "parazitoidi", "hiperapazitoidi", "agroekosistemi", "predeona kompleksnost", "efekat ivice", "biološka kontrola" ]

[ "aphids", "parasitoids", "hyperparasitoids", "landscape complexity", "edge effect", "biological control" ]

[ "Predeono-ekološka analiza diverziteta i interakcija sistema žitne vaši - parazitoidi (Homoptera; Hymenoptera)", "Landscape scale study of diversity and interaction in cereal aphids - parasitoids system (Homoptera; Hymenoptera)" ]

[ "" ]

[ "BY-NC-SA" ]

[ "http://nardus.mpn.gov.rs/bitstream/id/2313/Disertacija.pdf", "https://nardus.mpn.gov.rs/bitstream/id/2313/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2105" ]

[ "" ]

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[ "" ]

[ "Марковић Јовановић, Снежана" ]

[ "" ]

[ "2016-02-17T14:43:34", "2020-07-04T13:46:15" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/4518" ]

[ "" ]

[ "" ]

[ "" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc/4.0/" ]

[ "" ]

[ "" ]

[ "Вредности витамина Д код деце са бронхоопструкцијама" ]

[ "" ]

[ "BY-NC" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/60490/zorica_zivkovic_dd.pdf", "http://nardus.mpn.gov.rs/bitstream/id/60491/izvestaj_zorica_vujnovic_zivkovic.pdf", "https://nardus.mpn.gov.rs/bitstream/id/60491/izvestaj_zorica_vujnovic_zivkovic.pdf", "http://nardus.mpn.gov.rs/bitstream/id/60490/zorica_zivkovic_dd.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_4518" ]

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[ "" ]

[ "Stamenković, Gorana" ]

[ "Savić-Pavićević, Dušanka", "Knežević, Aleksandra M." ]

[ "2016-01-05T11:48:00", "2020-07-03T08:10:11" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/2136", "http://eteze.bg.ac.rs/application/showtheses?thesesId=1058", "https://fedorabg.bg.ac.rs/fedora/get/o:7742/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024586674" ]

[ "Uvod: Infekcija parvoB19 virusom koja se prenosi respiratornim i vertikalnim putem, ali i preko zaražene krvi i krvnih derivata, može predstavljati ozbiljan zdravstveni problem za rizične grupe pacijenata. Ovaj, po dimenzijama mali DNK virus bez omotača je vrlo otporan na standardne tehnike inaktivacije virusa u proizvodnji derivata krvi, a pouzdanu dijagnostiku otežava varijabilnost genomske sekvence virusa. Određivanje karakteristika nukleotidnih sekvenci izolata B19 virusa od značaja je za razvoj dijagnostičkih metoda i daje uvid u puteve nastanka i održavanja genomske varijabilnosti B19 virusa. Cilj ovog rada bio je da se odredi učestalost B19 virusa kod dobrovoljnih davalaca krvi i kod pacijenata sa sumnjom na B19 infekciju i da se na osnovu molekularno genetskih karakteristika dobijenih genomskih sekvenci, a u poređenju sa genomskim sekvencama dostupnim u bazama podataka, odredi evolutivna dinamika varijabilnosti parvoB19 virusa i filogenetski i filogeografski odnosi ispitivanih B19 izolata. Metode: Uzorci plazme dobrovoljnih davalaca i plazme i krvi pacijenata sa sumnjom na B19 infekciju analizirani su na prisustvo B19 DNK primenom lančane reakcije polimeraze (PCR), kako metodama iz literature tako i razvijenom internom metodom. Za uzorke sa detektovanim B19 genomom, direktnim nukleotidnim sekvenciranjem određena je genomska sekvenca. Bioinformatičkom analizom u odnosu na genomske sekvence B19 virusa dostupne u bazama podataka određen je genotip izolovanih virusnih genoma, evolutivni pritisci na osnovu odnosa nesinonimnih i sinonimnih zamena (dN/dS) za nukleotidne sekvence koje kodiraju virusne proteine, kao i genetičke distance i filogenetski odnosi B19 izolata dobijenih u Srbiji u odnosu na izolate iz drugih geografskih regija. Rezultati: Za 5 izolata iz Srbije, utvrđena je sekvenca B19 genoma u regiji kompletne cDNK. Iz baze podataka izdvojeni su i analizirani izolati sa poznatom sekvencom u regiji kompletne ili skoro kompletne cDNK, kojih je do 2012. godine bilo ukupno 146, od toga 117 genotipa 1. Analizom odnosa dN/dS za virusne proteine na osnovu većeg broja nukleotidnih sekvenci u odnosu na dosadašnje literaturne podatke procenjen je uticaj pozitivne i negativne selekcije na varijabilnost virusnog genoma..." ]

[ "Abstract: Background: Parvovirus B19 infection, being transmitted via the respiratory route, vertically from mother to fetus as well as through blood-derived products administered parenterally, can represent a serious health problem for risk patients groups. This small non-enveloped DNA virus is extremely resistant to standard viral inactivation techniques, while a reliable diagnosis is challenged by the virus genome sequence variability. Characterization of B19 isolates nucleotide sequences is valuable for development of diagnostic methods, and it gives an insight into the genesis and maintenance of the B19 virus genome variability. Aim of this thesis was to determine the frequency of B19 viremia in voluntary blood donors and in patients with the B19 infection symptoms, investigate the level and evolutionary dynamics of the B19 genome variability as well as to examine phylogenetic and phylogeographic relations of the B19 genomes obtained in Serbia with the isolates from the other parts of the world on the bases of the almost complete B19 cDNA sequences. Methods: Plasma samples of the voluntary blood donors, and plasma and blood samples of the B19 infection suspected patients were analysed for the B19 DNA presence by the polymerase chain reaction (PCR), using the methods described in the literature as well as internally developed method. For the B19 genome positive samples genome sequences were determined by direct sequencing. Bioinformatic analysis was performed by using the generated data as well as the database information in order to determine a genotype of the isolated genomes, evolutionary pressures (based on dN/dS ratio) for virus protein coding sequences, and genetic distances and phylogenetic relations of the B19 isolates from Serbia compared to the other geographic regions. Results: For 5 isolates from Serbia, the B19 genome sequence was determined for the complete cDNA. Database isolates with the known, complete or almost complete cDNA, available until the end of 2012 (146 isolates in total, out of which 117 were genotype 1) were chosen and used for the analysis. Positive and negative selection influence for the virus protein coding sequences was determined by the dN/dS ratio analysis by using larger data set than described in the literature so far..." ]

[ "" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "parvovirus B19", "genomska varijabilnost", "virusni genotipovi", "nukleotidna distanca", "filogenetski i filogeografski odnosi" ]

[ "parvovirus b19", "genome variability", "virus genotypes", "nucleotide distance", "phylogenetic and phylogeographic relations" ]

[ "Varijabilnost genoma humanog virusa Parvovirus B19 (Parvoviridae, Erythrovirus)", "Genome variability of Human Parvovirus B19 (Parvoviridae, Erythrovirus)" ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/2258/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2258/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2136" ]

[ "" ]

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[ "" ]

[ "" ]

[ "" ]

[ "" ]

[ "" ]

[ "Anđelković, Marko" ]

[ "Stamenković-Radak, Marina", "Kurbalija-Novičić, Zorana", "Ludoški, Jasmina" ]

[ "2016-01-05T11:45:24", "2020-07-03T08:10:45" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=86", "https://nardus.mpn.gov.rs/handle/123456789/2026", "https://fedorabg.bg.ac.rs/fedora/get/o:3490/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024538802" ]

[ "Drosophila subobscura je vrsta sa širokim Palearktičkim rasprostranjenjem. Naseljava gotovo ceo evropski kontinent, severnu Afriku, Malu Aziju i neka atlanska ostrva. Genetička struktura prirodnih populacija ove vrste je opisana upotrebom nekoliko genetičkih markera. Najtemeljnije je izučena varijabilnost hromozomskog inverzionog polimorfizma, po kome populacije D. subobscura pokazuju prostornu i vremensku struktuiranost. Klinalni obrasci ovog tipa varijabilnosti upućuju na značaj selektivnih procesa u njenom oblikovanju, dok razlike između geografski bliskih područja između kojih postoji geografska barijera upućuju na značaj istorijskih procesa. Naime, uočena varijabilnost oslikava procese rekolonizacije Evrope nakon završetka poslednje glacijacije iz primarnog pribežišta lociranog u regionu koji obuhvata Balkan, Malu Aziju i Kavkaz, ali i sekundarnog u regionu Pirinejskog poluostrva. Mikrosatelitska varijabilnost izučavana je u nekoliko evropskih populacija D. subobscura, uglavnom iz zapadnog dela Evrope. Pokazano je da su populacije slabo struktuirane po ovom genetičkom markeru. Populacije u južnom delu areala odlikuju se nešto većom genetičkom raznovrsnošću. Donekle je slična i slika varijabilnosti prirodnih populacija po mitohondrijalnoj DNK (mtDNK), u slučaju koje je zabeležena homogenost distribucije haplotipova između populacija. U velikoj većini do sada analiziranih populacija zabeleženo je prisustvo dva dominantna haplotipa i veliki broj endemičnih haplotipova niske učestalosti. Pokazano je da nekoliko faktora oblikuje varijabilnost mtDNK D.subobscura. Među njima su najznačajniji istorijski procesi, genetički drift, i prirodna selekcija koja najverovatnije deluje preko cito-nukleusne koadaptacije između alelskih kombinacija u okviru inverzija i mtDNK varijanti..." ]

[ "Drosophila subobscura shows a wide Palearctic distribution. It inhabits most of Europe, Northern Africa, Asia Minor and some Atlantic islands. The genetic structure in natural populations of this species has been extensively studied with respect to several genetic markers. The variability of chromosomal inversion polymorphism has been most thoroughly studied showing spatial and temporal structure in natural populations. The clinal pattern of chromosomal variability suggests the action of natural selection on inversion polymorphism, while abrupt changes in gene arrangement frequencies between closely related populations separated by geographical barrier suggest the importance of historical processes on the genetic structure. After the decay of the ice caps, Drosophila subobscura, most probably, recolonized Europe mainly from the Balkan Peninsula, Asia Minor and Caucasus, but also, to a lesser degree, from the Iberian Peninsula. Several populations of D. subobscura, mostly from the Western Europe, have been studied with respect to microsatellite variability. The analysis showed absence of structuring of genetic variation, with slightly higher genetic variability in Southern populations. There is a widespread genetic homogeneity of mitochondrial DNA (mtDNA) variability throughout the species range with high prevalence of two almost equally frequent haplotypes, and a number of population specific, rare haplotypes. Several factors account for the observed distribution in mtDNA variability: historical processes, genetic drift and natural selection, most probably acting through cytonuclear coadaptation of alleles inside inversions and mtDNA haplotypes..." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173012/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "Drosophila subobscura", "pribežište", "inverzioni polimorfizam", "mikrosateliti", "mtDNK", "cito-nukleusne koadaptacije" ]

[ "Drosophila subobscura", "refugia", "inversion polymorphism", "microsatellites", "mtDNA", "cyto-nuclear coadaptations" ]

[ "Hromozomska, mikrosatelitska i mitohondrijalna DNK varijabilnost populacija Drosophila subobscura u klisurama i kanjonima Srbije", "Chromosomal, microsatellite and mitochondrial DNA variability of Drosophila subobscura population from gorges and canyons in Serbia" ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/2372/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2372/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2026" ]

[ "" ]

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[ "" ]

[ "Mišić, Danijela" ]

[ "Sabovljević, Aneta", "Živković, Suzana", "Ćirić, Ana" ]

[ "2016-01-05T11:47:58", "2020-07-03T08:10:25" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/2132", "http://eteze.bg.ac.rs/application/showtheses?thesesId=790", "https://fedorabg.bg.ac.rs/fedora/get/o:7112/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=44652047" ]

[ "Nepeta rtanjensis Diklić i Milojević, N. sibirica L. i N. nervosa Royle ex Bentham (fam. Lamiaceae) su endemične vrste roda Nepeta, koje su nedovoljno fitohemijski okarakterisane i slabo ispitane u pogledu biološke aktivnosti. Radi se o vrstama koje se odlikuju različitim kvalitativnim i kvantitativnim sadržajem nepetalaktona, zbog čega predstavljaju idealne objekte u istraživanjima biološke aktivnosti različitih izomera ovog monoterpenskog jedinjenja. S obzirom da se radi o retkim vrstama, a u slučaju N. rtanjensis i o krajnje ugroženoj vrsti, biljni materijal za potrebe eksperimenata je dobijen mikropropagacijom. Istraživanje je obuhvatilo fitohemijske analize sekundarnih metabolita tri vrste roda Nepeta gajenih in vitro, kao i analizu njihovih bioaktivnih svojstava kao što su antimikrobno, antioksidativno i alelopatsko dejstvo. Fitohemijska karakterizacija tri vrste roda Nepeta gajenih in vitro, omogućila je identifikaciju i kvantifikaciju glavnih grupa sekundarnih metabolita (terpenoida i fenolnih jedinjenja) koji se u ovim uslovima produkuju. Metode koje su korišćene u fitohemijskim analizama su: GC-MS, GC-FID, PTR-MS, Headspace GC-MS, HPLCUV, HPLC-MS, UHPLC/DAD/±HESI-MS/MS. Dominantno isparljivo jedinjenje iz grupe terpenoida je monoterpenski lakton - nepetalakton, pri čemu je kod vrste N. rtanjensis većinski prisutan tran,cis- izomer a kod N. sibirica cis,trans- izomer nepetalaktona. Kod vrste N. nervosa nepetalakton je detektovan samo u tragovima. Kod ispitivanih vrsta, ruzmarinska kiselina se javlja kao dominantno fenolno jedinjenje, dok su ostale fenolne kiseline (hlorogena, neohlorogena i kafeinska kiselina) prisutne u znatno nižoj koncentraciji. Metanolni ekstrakti N. rtanjensis, N. sibirica i naročito N. nervosa, pokazuju značajnu antioksidativnu aktivnost u ABTS i DPPH testovima, kao i visoki kapacitet za redukciju gvožđa u FRAP testu. Značajna antioksidativna aktivnost se pripisuje fenolnim jedinjenjima, na prvom mestu ruzmarinskoj kiselini..." ]

[ "Nepeta rtanjnsis Diklić and Milojević, N. sibirica L. and N. nervosa Royle ex Bentham (fam. Lamiaceae) are endemic species of genus Nepeta that are insufficiently phytochemically characterized and poorly tested in a sense of their biological activity. These three species are characterized by different qualitative and quantitative nepetalactone content, what makes them ideal objects in investigating the biological activity of different stereoisomers of this compound. Considering that these species are rare, and extremely endangered in case of N. rtanjensis, plant material for the experiments was obtained by micropropagation. The research included phytochemical characterization of in vitro grown three Nepeta species , as well as analysis of their bioactivities such as antimicrobial, antioxidative activity and allelopathic potential. Phytochemical characterization of three Nepeta species grown in vitro, involving GC-MS, GC-FID, PTR-MS, Headspace GC-MS, HPLC-UV, HPLC-MS, and UHPLC/DAD/±HESI-MS/MS analyses, enabled identification and quantification of the main secondary metabolites (terpenes and phenolics). The dominant volatile compound from terpenes group is monoterpene lacton - nepetalactone, whereby its trans,cisisomer is mostly present in N. rtanjensis and its cis,trans- isomer in N. sibirica. In N. nervosa, nepetalactone has been detected only in traces. In all investigated species, rosmarinic acid is a dominant phenolic compound, while the other phenolic acids (chlorogenic, neochlorogenic and caffeic acids) are present in significantly lower concentrations. The results of ABTS and DPPH assays showed that methanol extracts of N. rtanjensis, N. sibirica and especially N. nervosa, possess considerable antioxidant activities, and the FRAP assay revealed high ferric reducing capacity for all the samples tested.. Significant antioxidant activity is attributed to phenolic acids, in the first place to rosmarinic acid." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173024/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by/4.0/" ]

[ "Nepeta rtanjensis", "Nepeta sibirica", "Nepeta nervosa", "nepetalakton", "fenolne kiseline", "antioksidativni potencijal", "antimikrobno dejstvo", "alelopatija", "antioksidativni enzimi" ]

[ "Nepeta rtanjensis", "Nepeta sibirica", "Nepeta nervosa", "nepetalactone", "phenolic acid", "antioxidative potential", "antimicrobial effects", "allelopathy", "antioxidant enzyme" ]

[ "Antioksidativno, antimikrobno i alelopatsko dejstvo tri endemične vrste roda Nepeta (Lamiaceae)", "Antioxidative, antimicrobial and allelopathic effects of three endemic Nepeta species (Lamiaceae)" ]

[ "" ]

[ "BY" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/2305/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2305/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2132" ]

[ "" ]

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[ "Mijatović, Sanja" ]

[ "Mijatović, Sanja", "Matić, Gordana", "Stošić-Grujičić, Stanislava", "Maksimović-Ivanić, Danijela" ]

[ "2016-01-05T11:46:41", "2020-07-03T08:10:22" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=169", "https://nardus.mpn.gov.rs/handle/123456789/2082", "https://fedorabg.bg.ac.rs/fedora/get/o:5402/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024570546" ]

[ "Iz poznate uzročno-posledične veze infekcije i inflamacije, s jedne strane i karcinogeneze i progresije tumora, s druge strane, proistekla je ideja o upotrebi anti-inflamatornih i anti-infektivnih agenasa u terapiji kancera. Međutim, problem gastrotoksičnosti anti-inflamatornih, kao i generalne toksičnosti anti-viralnih lekova uz činjenicu da je reč o agensima niske biološke iskoristljivosti, ozbiljne su barijere u razmatranju njihove primene u terapiji bolesti za koje inicijalno nisu namenjeni. Kako bi se prevazišla farmakološka ograničenja pomenutih jedinjenja, a imajući u vidu poznato svojstvo azot-monoksida (NO) da neutrališe toksičnost supstanci na različitim nivoima, anti-inflamatornom agensu VGX-1027 i anti-retroviralnom agensu sakvinaviru (Saq) adekvatnom hemijskom intervencijom je dodata grupa dizajnirana da donira NO. Prednost novodobijenih jedinjenja GIT-27NO i Saq-NO nad poznatim nesteroidnim anti-inflamatornim agensima modifikovanim kovalentnim vezivanjem NO (NO-NSAID) je odsustvo molekula “nosača”, koji je inače odgovoran za genotoksičnost NO-NSAID. Strukturna promena ova dva jedinjenja doprinela je njihovom snažnom antitumorskom svojstvu, što je potvđeno brojnim in vitro i in vivo studijama, uz umanjenu toksičnost u slučaju GIT-27NO ili potpunom gubitku toksičnosti Saq-NO tretmana.U ovoj studiji je po prvi put ispitano antitumorsko delovanje GIT-27NO i Saq-NO in vitro i in vivo na modelu kancera kolona, jednog od najtežih formi maligniteta. Kako su oba agensa NO-derivati, definisana je uloga NO u njihovom antitumorskom delovanju. Na molekularnom nivou, determinisani su glavni unutarćelijski događaji pokrenuti u odgovoru na tretman pomenutim jedinjenjima. Na kraju, imajući u vidu značaj mikrosredine za rast i progresiju tumora, ispitana je sposobnost GIT-27NO i Saq-NO da povrate osetljivost ćelija kancera kolona na antitumorski imunski odgovor posredovan TRAIL molekulom." ]

[ "Concept of using anti-inflammatory and anti-infectious agents in cancer therapy is based on a known relationship between inflammation and infection on one side and carcinogenesis and tumor progression on the other side. However, the problem of gastrotoxicity of anti-inflammatory and general toxicity of antiviral drugs, along with their low bioavailability, marks a strong barrier when considering them in therapies for which they were not initially intended for. In order to overcome the pharmacological limits of these compounds, nitric-oxide (NO) with its documented feature to neutralize toxicity of drugs on various levels was added to anti-inflammatory agent VGX-1027 and antiretroviral agent saquinavir (Saq). The advantage of new compounds, GIT-27NO and Saq-NO, over known non-steroid anti-inflammatory agents modified by covalent binding NO moiety (NO-NSAID) is the absence of “carrier” molecule, which is responsible for genotoxicity of NO-NSAID. Structural change in these compounds resulted in potentiation of antitumor action, confirmed by numerous in vitro and in vivo studies, along with reduced toxicity of GIT-27NO or complete loss of toxicity of Saq-NO treatment. In this study, antitumor potential of GIT-27NO and Saq-NO was tested in vitro and in vivo for the first time in colon cancer model, one of the most severe forms of malignancy. As both agents are NO-derivates, the role of NO in their antitumor action was defined. On molecular level, main intercellular events triggered by the treatment were determined. Finally, considering the importance of microenvironment for tumor growth and progression, the ability of GIT-27NO and Saq-NO to re-establish colon cancer cell sensitivity to TRAIL-mediated antitumor immune response was tested. GIT-27NO and Saq-NO reduced the viability of mouse CT26CL25 and human HCT116 colon cancer cell lines, both in vitro and in vivo. The importance of NO release for antitumor action was quite different. While cell viability reduction under GIT-27NO treatment was due to accumulation of high intracellular concentration of NO and consecutively generated oxidative and nitrosative stress, antitumor action of Saq-NO was not mediated by a release of quantitatively relevant amount of this free radical. GIT-27NO induced the accumulation of p53 tumor suppressor, changed pro- and antiapoptotic molecule ratio and triggered mitochondrial membrane depolarization which resulted in cell death via caspase-dependent apoptosis." ]

[ "info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173013/RS//" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-sa/4.0/" ]

[ "Kancer kolona", "hemioterapija", "GIT-27NO", "Saq-NO", "apoptoza", "metastaze", "S6 protein", "TRAIL" ]

[ "Colon cancer", "chemotherapy", "GIT-27NO", "Saq-NO", "apoptosis", "metastasis", "S6 protein", "TRAIL" ]

[ "Analiza antitumorskog delovanja agenasa modifikovanih azot-monoksidom, Sakvinavir-NO i GIT-27NO na kanceru kolona in vitro i in vivo", "In vitro and in vivo evaluation of antitumor activity of NO-modified compounds, Saquinavir-NO and GIT-27NO in colon cancer" ]

[ "" ]

[ "BY-NC-SA" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/2293/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2293/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2082" ]

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[ "Radović, Svetlana" ]

[ "Radović, Svetlana", "Matić, Gordana", "Maksimović, Vesna" ]

[ "2016-01-05T11:48:05", "2020-07-03T08:10:17" ]

[ "http://eteze.bg.ac.rs/application/showtheses?thesesId=1073", "https://nardus.mpn.gov.rs/handle/123456789/2140", "https://fedorabg.bg.ac.rs/fedora/get/o:7778/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024590258" ]

[ "Jedan od glavnih faktora abiotičkog stresa kod biljaka je dehidratacija. U ovom radu ispitivan je uticaj dehidratacije na ekspresiju gena za transkripcioni faktor DREB2A i malih RNK miR398 i miR408 graška, u cilju sagledavanja značaja ovih molekula u odgovoru graška na dehidrataciju i rehidrataciju. Parcijalna cDNK (PsDREB2A) koja kodira za DREB2A transkripcioni faktor graška je dobijena primenom metode PCR sa prajmerima dizajniranim na osnovu sekvenci DREB2A gena iz model leguminoze Medicago truncatula. Dobijena sekvenca (495 bp) deponovana je u GeneBank (HM229349). PoreĎenje nukletidnih sekvenci pokazalo je da je dobijena sekvenca najsličnija sa sekvencom za DREB2A iz M. truncatula, kao i sa EREBP (ethylene responsive element binding protein) iz iste vrste. Analiza izvedene aminokiselinske sekvence pokazala je da PsDREB2A ima strukturu karakterističnu za DREB2A protein - prisustvo konzervisanog AP2 domena (17-55 ak), kao i da ima najveću homologiju sa DREB2A i sa ERBP proteinom iz M. truncatula. Na osnovu kompjuterske predikcije posttranslacionih modifikacija proteina utvrĎeno je prisustvo jednog potencijalnog mesta za sumoilaciju proteina, i to na poziciji Lys157 (MKQE), kao i prisustvo nekoliko potencijalnih mesta za fosforilaciju, najverovatnije protein kinazom A. U cilju ispitivanja ekspresije gena za DREB2A transkripcioni factor graška vršene su analize iRNK korena i nadzemnog dela graška u normalnom fiziološkom stanju, kao i u uslovima dehidratacije u trajanju 7 i 10 dana, i rehidratacije. Primenjena je metoda Real-time PCR uz upotrebu proba specifičnih za PsDREB2A. Pokazano je da se profil ekspresije razlikuje u korenu i nadzemnom delu biljaka. Kod korena maksimalni nivo ekspresije PsDREB2A utvrĎen je nakon 10 dana tretmana, dok je kod nadzemnog dela biljaka maksimum dostignut nakon 7 dana dehidratacije..." ]

[ "Adverse environmental stresses, such as drought, low temperature and soil salinity have a strong influence on agricultural production and sustainability. A major limitation to yield and quality in many crop species is water availability throughout or at critical times during the growing season. We investigated the effect of dehydration on expression of DREB2A transcription factor in pea, as well as on the expression of conserved miRNAs-miR398a/b and miR408. Partial cDNA (PsDREB2A), which encodes a DREB2A transcription factor in pea, was obtained by PCR with primers designed according to DREB2A gene from the model legume Medicago truncatula. The resulting sequence (495 bp) has been deposited in Gene Bank (HM229349). Comparison of nucleotide sequences showed that the obtained sequence is most similar to the sequence of DREB2A from M. truncatula, and with EREBP (ethylene responsive element binding protein) from the same species. Analysis of deduced amino acid sequence showed that PsDREB2A has a structure characteristic of the DREB2A protein - presence of AP2 domains (17-55 a.a.), and has the highest homology with DREB2A and ERBP protein from M. truncatula. Computer prediction of post-translational modification of protein revealed the presence of a potential site for protein sumoylation, at the position of Lys157 (MKQE), and the presence of several potential sites for phosphorylation. To study the expression of PsDREB2A we performed the analysis of mRNA level in pea root and shoot in a normal physiological state, as well as during dehydration for 7 and 10 days, and rehydration. Real-time PCR using a probe specific for PsDREB2A has been applied. It has been shown that the expression profile was different in roots and aerial parts of the plants. In the roots the maximum level of expression PsDREB2A was determined after 10 days of treatment, while in the above-ground parts of plants the maximum was rached after 7 days of dehydration..." ]

[ "" ]

[ "openAccess" ]

[ "https://creativecommons.org/licenses/by-nc-nd/4.0/" ]

[ "dehidratacija", "grašak", "transkripcioni faktor", "DREB2A", "male RNK", "miR398", "miR408" ]

[ "dehydration", "pea", "transcription factor", "DREB2A", "small RNA", "miR398", "miR408" ]

[ "Uloga transkripcionog faktora DREB2A i malih RNK miR398a/b i miR408 u odgovoru graška (Pisum sativum L.) na dehidrataciju", "The role of DREB2A transcription factor and small RNAsmiR398a/b and miR408 in dehidratation response of pea (Pisum sativum L.)" ]

[ "" ]

[ "BY-NC-ND" ]

[ "https://nardus.mpn.gov.rs/bitstream/id/2274/Disertacija.pdf", "http://nardus.mpn.gov.rs/bitstream/id/2274/Disertacija.pdf" ]

[ "https://hdl.handle.net/21.15107/rcub_nardus_2140" ]

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[ "Budimir, Snežana" ]

[ "Janošević, Dušica", "Simonović, Ana" ]

[ "2016-01-05T11:45:16", "2020-07-03T08:10:31" ]

[ "https://nardus.mpn.gov.rs/handle/123456789/2013", "http://eteze.bg.ac.rs/application/showtheses?thesesId=43", "https://fedorabg.bg.ac.rs/fedora/get/o:2416/bdef:Content/download", "http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=41541135" ]

[ "Sеnеscеnciја listа prеdstаvlја kоmplеksnu, gеnеtički kоntrоlisаnu pоslеdnju fаzu оntоgеnеzе listа kоја rеzultirа kооrdinisаnоm rаzgrаdnjоm mаkrоmоlеkulа i mоbilizаciјоm njеgоvih kоmpоnеnti u drugе dеlоvе bilјkе. Nа ćеliјskоm nivоu, оnа оbuhvаtа prоmеnе strukturе, mеtаbоlizmа i gеnskе еksprеsiје kоје dоvоdе dо smrti, čiјi sе tip оznаčаvа kао prоgrаmirаnа ćеliјskа smrt (PCD). Kао еkspеrimеntаlni mоdеl u оvоm rаdu kоrišćеnе su in vitro gајеnе bilјkе duvаnа (Nicotiana tabacum L.) kоје оbеzbеđuјu sеt listоvа u kојimа pоstојi grаdаciја sеnеscеnciје. Istrаživаnjе је оbuhvаtilо citоhistоlоšku i imunоhistоhеmiјsku аnаlizu dinаmikе strukturnih prоmеnа mеzоfilа listоvа, kао i ispitivаnjе mеtаbоličkih, biоhеmiјskih i mоlеkulаrnih prоcеsа tоkоm sеnеscеnciје listа. Utvrđеn је rеdоslеd dеgеnеrаtivnih prоmеnа u strukturi ćеliја tоkоm fiziоlоškоg stаrеnjа listоvа. U sеnеscеntnim listоvimа dеtеktоvаni su tipični simptоmi prоgrаmirаnе ćеliјskе smrti, kоја pо svојim mоrfоlоškim оdlikаmа оdgоvаrа аutоfаgnој ćеliјskој smrti, i pоkаzаnо dа intеrnuklеоzоmаlnа frаgmеntаciја DNK prеthоdi ultrаstrukturnim prоmеnаmа kао štо su dеgrаdаciја hlоrоplаstа i kоndеnzаciја hrоmаtinа. Sаdržај hlоrоfilа i citоsоlnih prоtеinа idеntifikоvаni su kао pоuzdаni biоmаrkеri stеpеnа sеnеscеnciје listоvа. Hеmiјskоm аnаlizоm еndоgеnih rеgulаtоrа rаstеnjа idеntifikоvаnо је i kvаntifikоvаnо višе оd 20 rаzličitih citоkininа i pоkаzаnо dа је nаprеdоvаnjе sеnеscеnciје u kоrеlаciјi sа оpаdаnjеm sаdržаја ukupnih citоkininа, kао i sа smаnjеnjеm nivоа citоkininа trans-zеаtinskоg tipа. Таkоđе је uоčеnа јаsnа kоrеlаciја izmеđu smаnjеnjа nivоа еndоgеnе IAA, оdnоsnо ABA i nаprеdоvаnjа sеnеscеnciје. Imunоhistоhеmiјskоm аnаlizоm mеzоfilа listоvа bilјаkа trеtirаnih citоkininimа pоkаzаnо је dа visоkе dоzе citоkininа BА ubrzаvајu sеnеscеnciјu, čimе indukuјu prоgrаmirаnu smrt ćеliја. Аnаlizа аktivnоsti pојеdinih еnzimskih kоmpоnеnti аntiоksidаtivnоg sistеmа pоkаzаlа је znаčајnе prоmеnе u аntiоksidаtivnоm stаtusu listоvа tоkоm fiziоlоškоg stаrеnjа. Nаprеdоvаnjе sеnеscеnciје је u јаsnој kоrеlаciјi sа smаnjеnjеm аktivnоsti CAT i pоvеćаnjеm ukupnе аktivnоsti POD. Primаrnu аntiоksidаtivnu zаštitu tоkоm stаrеnjа оbеzbеđuјu SOD, а u prоcеs оdbrаnе оd štеtnоg dејstvа ROS u iniciјаlnој fаzi sеnеscеnciје uklјučuјu sе POD. Моlеkulаrnа аnаlizа sеnеscеnciје pоkаzаlа је аktivаciјu gеnа kојi kоdirајu еnzimе uklјučеnе u kоnvеrziјu i rеmоbilizаciјu prоdukаtа rаzlаgаnjа u sеnеscеntnim listоvimа. qRT-PCR mеtоdоm pоkаzаnа је indukciја еksprеsiје gеnа kојi kоdirајu cistеin prоtеаzе оdgоvоrnе zа rеmоbilizаciјu аminоkisеlinа, kао i cistеin prоtеаzu iz grupе mеtаkаspаzа uklјučеnu u kоntrоlu ćеliјskе smrti. Indukciја еksprеsiје Gln1-3 i GDH2, SAG-gеnа uklјučеnih u mоbilizаciјu аzоtа u sеnеscеntnim listоvimа, nаpоrеdо sа pаdоm еksprеsiје GS2 ukаzаlа је dа sе аsimilаciја аmоniјumа tоkоm sеnеscеnciје pоstеpеnо prеmеštа iz hlоrоplаstа u citоsоl mеzоfilnih ćеliја." ]

[ "Leaf senescence constitutes the final stage of leaf development that results in the coordinated degradation of macromolcules and the subsequent nutrient relocation to other plant parts. Leaf senescence involves an integrated action at the cellular, tissue, organ and organism levels under the control of a highly regulated genetic program. During senescence, leaf cells undergo orderly changes in cell structure, metabolism and gene expression leading to cell death, recognized as a form of programmed cell death (PCD). In vitro grown Nicotiana tabacum L. has been chosen as a model plant system, since a single plant provides a large set of leaves in which there is a gradation of senescence. The dynamics of ultrastructural changes in leaf mesophyll cells, as well as metabolic, biochemical and molecular changes during leaf senescence were investigated. We determined the time sequence of senescence-related events in mesophyll cells during physiological ageing of leaves. Typical symptoms of autophagic PCD were detected in senescing leaves using cytological analysis and TUNEL assay. We showed that PCD-specific nuclear DNA fragmentation occurs during natural tobacco leaf senescence but that it starts early in leaf development, thus preceeding ultrastructural changes such as chloroplast degradation and chromatin condensation. Chlorophyll and soluble protein content were identified as reliable biomarkers for estimating the relative age of a particular leaf along the main plant axis. Using HPLC/MS more than 20 different cytokinins were identified and quantified in tobacco leaves. The correlation of senescence with decline in total cytokinin content and the levels of trans-Z was found. The progression of leaf senescence correlated with a gradual decrease in endogenous levels of both IAA and ABA. Immunohistological analysis showed that high levels of cytokinin benzyladenine accelerate senescence thus inducing PCD. The activities of different antioxidative enzymes were analyzed during physiological ageing of leaves. Progression of senescence is correlated with decrease in CAT activity and a concomitant increase in POD activity. Primary antioxidative protection in the course of ageing is provided by SOD, whereas POD are implicated in defense against detrimental ROS action in an initial phase of senescence. Molecular analysis of leaf senescence has revealed activation of genes encoding enzymes involved in conversion and remobilization of degradation products in senescing leaves. Quantitative RT-PCR showed that the expression of cysteine proteases responsible for amino acid remobilization, typically associated with leaf senescence and not found in other forms of PCD, was induced in senescent leaves. Induced expression of SAGs involved in nitrogen mobilization in senescing leaves, Gln1-3 и GDH2, contiguous with decrease in GS2 expression indicated that, during senescence, ammonia assimilation is progressively shifted from the chloroplasts to the cytosol of leaf mesophyll cells." ]