jackkuo/LLM-Enzyme-Kinetics-Golden-Benchmark

ID_gold int64 1 4.37k	pubmed_id int64 1.88M 33.4M	Enzyme stringclasses 283 values	Organism stringclasses 232 values	Substrate stringlengths 1 81 ⌀	km(mM) stringlengths 1 8 ⌀	kcat(s^-1) stringlengths 1 10 ⌀	km_kcat(mM^-1s^-1) stringlengths 1 12 ⌀	Commentary[Temp] stringclasses 70 values	Commentary[pH] stringclasses 102 values	Commentary[Mutant] stringclasses 806 values	Commentary[Cosubstrate] stringclasses 200 values	Reference[Table/Text] stringclasses 4 values
1	11,900,558	BglB	Pyrococcus horikoshii	pNP-Glc	13.5	34.4	2.58	90°C	4.75	WT	0.1 M Sodium Citrate/0.1 M NaPi	Table
2	11,900,558	BglB	Pyrococcus horikoshii	pNP-Gal	7.3	13.5	1.9	90°C	4.75	WT	0.1 M Sodium Citrate/0.1 M NaPi	Table
3	11,900,558	BglB	Pyrococcus horikoshii	pNP-Man	0.44	4.2	9.8	90°C	4.75	WT	0.1 M Sodium Citrate/0.1 M NaPi	Table
4	11,900,558	BglB	Pyrococcus horikoshii	pNP-Glc	16.2	25.5	1.6	90°C	4.75	Q77R	0.1 M Sodium Citrate/0.1 M NaPi	Table
5	11,900,558	BglB	Pyrococcus horikoshii	pNP-Gal	16.8	14.2	0.85	90°C	4.75	Q77R	0.1 M Sodium Citrate/0.1 M NaPi	Table
6	11,900,558	BglB	Pyrococcus horikoshii	pNP-Man	1.1	1.6	1.4	90°C	4.75	Q77R	0.1 M Sodium Citrate/0.1 M NaPi	Table
7	11,900,558	BglB	Pyrococcus horikoshii	pNP-Glc	0.3	252	870	90°C	4.75	D206N	0.1 M Sodium Citrate/0.1 M NaPi	Table
8	11,900,558	BglB	Pyrococcus horikoshii	pNP-Gal	7.8	571	75.8	90°C	4.75	D206N	0.1 M Sodium Citrate/0.1 M NaPi	Table
9	11,900,558	BglB	Pyrococcus horikoshii	pNP-Man	3.2	45	14.7	90°C	4.75	D206N	0.1 M Sodium Citrate/0.1 M NaPi	Table
10	11,900,558	BglB	Pyrococcus horikoshii	pNP-Glc	30.4	439	14.4	90°C	4.75	Q77R/D206N	0.1 M Sodium Citrate/0.1 M NaPi	Table
11	11,900,558	BglB	Pyrococcus horikoshii	pNP-Gal	36.1	66.5	1.8	90°C	4.75	Q77R/D206N	0.1 M Sodium Citrate/0.1 M NaPi	Table
12	11,900,558	BglB	Pyrococcus horikoshii	pNP-Man	0.68	6.8	9.9	90°C	4.75	Q77R/D206N	0.1 M Sodium Citrate/0.1 M NaPi	Table
13	11,900,558	CelB	Pyrococcus furiosus	pNP-Glc	0.19	1140	7337	90°C	4.75	WT	0.1 M Sodium Citrate/0.1 M NaPi	Table
14	11,900,558	CelB	Pyrococcus furiosus	pNP-Gal	5	2827	561	90°C	4.75	WT	0.1 M Sodium Citrate/0.1 M NaPi	Table
15	11,900,558	CelB	Pyrococcus furiosus	pNP-Man	1.3	65.9	49.8	90°C	4.75	WT	0.1 M Sodium Citrate/0.1 M NaPi	Table
16	11,900,558	CelB	Pyrococcus furiosus	pNP-Glc	13.8	207	14.9	90°C	4.75	R77Q	0.1 M Sodium Citrate/0.1 M NaPi	Table
17	11,900,558	CelB	Pyrococcus furiosus	pNP-Gal	21.7	69.2	3.2	90°C	4.75	R77Q	0.1 M Sodium Citrate/0.1 M NaPi	Table
18	11,900,558	CelB	Pyrococcus furiosus	pNP-Man I	0.64	1.22	1.9	90°C	4.75	R77Q	0.1 M Sodium Citrate/0.1 M NaPi	Table
19	11,900,558	CelB	Pyrococcus furiosus	pNP-Man II	0.15	0.85	5.7	90°C	4.75	R77Q	0.1 M Sodium Citrate/0.1 M NaPi	Table
20	11,900,558	CelB	Pyrococcus furiosus	pNP-Gal	15.7	180	11.5	90°C	4.75	N206D	0.1 M Sodium Citrate/0.1 M NaPi	Table
21	11,900,558	CelB	Pyrococcus furiosus	pNP-Glc	4.6	116	24.9	90°C	4.75	N206D	0.1 M Sodium Citrate/0.1 M NaPi	Table
22	11,900,558	CelB	Pyrococcus furiosus	pNP-Man	0.63	1.97	3.1	90°C	4.75	N206D	0.1 M Sodium Citrate/0.1 M NaPi	Table
23	11,900,558	CelB	Pyrococcus furiosus	pNP-Glc	13.9	172	12.3	90°C	4.75	R77Q/N206D	0.1 M Sodium Citrate/0.1 M NaPi	Table
24	11,900,558	CelB	Pyrococcus furiosus	pNP-Gal	22	29.2	1.3	90°C	4.75	R77Q/N206D	0.1 M Sodium Citrate/0.1 M NaPi	Table
25	11,900,558	CelB	Pyrococcus furiosus	pNP-Man I	1.5	1.9	1.3	90°C	4.75	R77Q/N206D	0.1 M Sodium Citrate/0.1 M NaPi	Table
26	11,900,558	CelB	Pyrococcus furiosus	pNP-Man II	0.1	1.02	10.2	90°C	4.75	R77Q/N206D	0.1 M Sodium Citrate/0.1 M NaPi	Table
27	11,900,558	CelB	Pyrococcus furiosus	pNP-Glc	12	309	26	90°C	4.75	N206S	0.1 M Sodium Citrate/0.1 M NaPi	Table
28	11,900,558	CelB	Pyrococcus furiosus	pNP-Gal	200	1090	5.5	90°C	4.75	N206S	0.1 M Sodium Citrate/0.1 M NaPi	Table
29	11,900,558	CelB	Pyrococcus furiosus	pNP-Man	4.7	15	3.2	90°C	4.75	N206S	0.1 M Sodium Citrate/0.1 M NaPi	Table
30	19,004,819	SDH	S. novella	sulfite	0.0006	63.5	106000	25°C	6.0	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
31	19,004,819	SDH	S. novella	sulfite	0.0011	86.2	75500	25°C	6.5	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
32	19,004,819	SDH	S. novella	sulfite	0.0037	158.8	42700	25°C	7.0	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
33	19,004,819	SDH	S. novella	sulfite	0.0071	293.4	40300	25°C	7.5	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
34	19,004,819	SDH	S. novella	sulfite	0.022	345.3	15300	25°C	8.0	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
35	19,004,819	SDH	S. novella	sulfite	0.086	410	4880	25°C	8.5	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
36	19,004,819	SDH	S. novella	sulfite	0.324	519	1500	25°C	9.0	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
37	19,004,819	SDH	S. novella	sulfite	1.66	431	251	25°C	9.5	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
38	19,004,819	SDH	S. novella	sulfite	3.389	23.7	6.77	25°C	10.0	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
39	19,004,819	SDH	S. novella	sulfite	0.004	36.8	8420	25°C	6.0	Y236F	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
40	19,004,819	SDH	S. novella	sulfite	0.007	41.6	5600	25°C	6.5	Y236F	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
41	19,004,819	SDH	S. novella	sulfite	0.026	51.8	1970	25°C	7.0	Y236F	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
42	19,004,819	SDH	S. novella	sulfite	0.042	48.3	1140	25°C	7.5	Y236F	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
43	19,004,819	SDH	S. novella	sulfite	0.114	53.4	461	25°C	8.0	Y236F	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
44	19,004,819	SDH	S. novella	sulfite	0.332	58.3	173	25°C	8.5	Y236F	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
45	19,004,819	SDH	S. novella	sulfite	1.155	64	54.5	25°C	9.0	Y236F	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
46	19,004,819	SDH	S. novella	sulfite	4.456	52.7	11.6	25°C	9.5	Y236F	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
47	19,004,819	SDH	S. novella	sulfite	15.487	44.8	2.84	25°C	10.0	Y236F	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
48	19,004,819	SDH	S. novella	sulfite	0.812	70.5	86.8	25°C	5.8	R55M	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
49	19,004,819	SDH	S. novella	sulfite	1.087	64	58.9	25°C	6.2	R55M	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
50	19,004,819	SDH	S. novella	sulfite	1.63	64	39.3	25°C	6.6	R55M	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
51	19,004,819	SDH	S. novella	sulfite	1.95	66.3	34	25°C	7.0	R55M	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
52	19,004,819	SDH	S. novella	sulfite	3.62	68.3	18.9	25°C	7.5	R55M	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
53	19,004,819	SDH	S. novella	sulfite	8.17	73.4	8.98	25°C	7.9	R55M	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
54	19,004,819	SDH	S. novella	sulfite	33.27	109.88	3.3	25°C	8.3	R55M	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
55	19,004,819	SDH	S. novella	sulfite	0.667	238.8	358	25°C	6.0	H57A	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
56	19,004,819	SDH	S. novella	sulfite	0.29	226.5	782	25°C	6.5	H57A	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
57	19,004,819	SDH	S. novella	sulfite	0.189	214.5	1130	25°C	7.0	H57A	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
58	19,004,819	SDH	S. novella	sulfite	0.22	220.5	1010	25°C	7.5	H57A	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
59	19,004,819	SDH	S. novella	sulfite	0.27	214.6	799	25°C	8.0	H57A	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
60	19,004,819	SDH	S. novella	sulfite	0.452	222.4	492	25°C	8.5	H57A	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
61	19,004,819	SDH	S. novella	sulfite	1.46	192.5	132	25°C	9.0	H57A	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
62	19,004,819	SDH	S. novella	sulfite	12.2	148.4	12.2	25°C	9.5	H57A	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
63	19,004,819	SDH	S. novella	sulfite	0.0032	730	225000	10°C	6	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
64	19,004,819	SDH	S. novella	sulfite	0.0036	847	239000	10°C	6.5	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
65	19,004,819	SDH	S. novella	sulfite	0.0014	782	55800	10°C	7	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
66	19,004,819	SDH	S. novella	sulfite	0.0031	677	21800	10°C	7.5	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
67	19,004,819	SDH	S. novella	sulfite	0.0087	776	8890	10°C	8	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
68	19,004,819	SDH	S. novella	sulfite	0.412	829	2010	10°C	8.5	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
69	19,004,819	SDH	S. novella	sulfite	1.183	731	618	10°C	9	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
70	19,004,819	SDH	S. novella	sulfite	3.811	674	177	10°C	9.5	WT	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
71	19,004,819	SDH	S. novella	sulfite	4.53	746	165	10°C	5.5	R55M	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
72	19,004,819	SDH	S. novella	sulfite	6.48	662	102	10°C	6	R55M	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
73	19,004,819	SDH	S. novella	sulfite	22.8	240	10.5	10°C	7	R55M	20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c	Table
98	21,105,905	TCME1	Trypanosoma cruzi	L-Malate	0.13	518	3981	null	7.4	WT	NADP+(0.5), Mn2+(0.5)	Table
99	21,105,905	TCME1	Trypanosoma cruzi	NADP+	0.027	504	18681	null	7.4	WT	L-Malate (5), Mn2+(0.5)	Table
100	21,105,905	TCME1	Trypanosoma cruzi	Pyruvate	7.6	8.3	1.1	null	7.4	WT	NADPH(0.5), Mn2+(0.5), HCO3-(5)	Table
101	21,105,905	TCME1	Trypanosoma cruzi	Mg2+	0.04	461	11513	null	7.4	WT	L-Malate (5), NADP+(0.5)	Table
102	21,105,905	TCME1	Trypanosoma cruzi	Mn2+	0.0043	518	120361	null	7.4	WT	L-Malate (5), NADP+(0.5)	Table
103	21,105,905	TcME2	Trypanosoma cruzi	L-Malate	2.3	683	297	null	7.4	WT	NADP+(0.5), Mn2+(0.5)	Table
104	21,105,905	TcME2	Trypanosoma cruzi	NADP+	0.032	691	21599	null	7.4	WT	L-Malate (35), Mn2+(0.5)	Table
105	21,105,905	TcME2	Trypanosoma cruzi	Pyruvate	5.1	39	8	null	7.4	WT	NADPH(0.5), Mn2+(0.5), HCO3-(5)	Table
106	21,105,905	TcME2	Trypanosoma cruzi	Mg2+	0.57	864	1516	null	7.4	WT	L-Malate (35), NADP+(0.5)	Table
107	21,105,905	TcME2	Trypanosoma cruzi	Mn2+	0.0091	687	75479	null	7.4	WT	L-Malate (35), NADP+(0.5)	Table
108	21,105,905	TbME1	Trypanosoma brucei	L-Malate	0.32	1132	3536	null	7.4	WT	NADP+(0.5), Mn2+(0.5)	Table
109	21,105,905	TbME1	Trypanosoma brucei	NADP+	0.048	1206	25166	null	7.4	WT	L-Malate (5), Mn2+(0.5)	Table
110	21,105,905	TbME1	Trypanosoma brucei	Pyruvate	4.3	113	26	null	7.4	WT	NADPH(0.5), Mn2+(0.5), HCO3-(5)	Table
111	21,105,905	TbME1	Trypanosoma brucei	Mg2+	0.27	1280	4739	null	7.4	WT	L-Malate (5), NADP+(0.5)	Table
112	21,105,905	TbME1	Trypanosoma brucei	Mn2+	0.0024	905	377192	null	7.4	WT	L-Malate (5), NADP+(0.5)	Table
113	21,105,905	TbME2	Trypanosoma brucei	L-Malate	0.49	242	494	null	7.4	WT	NADP+(0.5), Mn2+(0.5)	Table
114	21,105,905	TbME2	Trypanosoma brucei	NADP+	0.016	284	17775	null	7.4	WT	L-Malate (5), Mn2+(0.5)	Table
115	21,105,905	TbME2	Trypanosoma brucei	Pyruvate	5.3	34	6	null	7.4	WT	NADPH(0.5), Mn2+(0.5), HCO3-(5)	Table
116	21,105,905	TbME2	Trypanosoma brucei	Mg2+	0.58	208	359	null	7.4	WT	L-Malate (5), NADP+(0.5)	Table
117	21,105,905	TbME2	Trypanosoma brucei	Mn2+	0.0066	246	37302	null	7.4	WT	L-Malate (5), NADP+(0.5)	Table
118	22,387,379	AzoA	Enterococcus faecalis	Methyl Red	0.024	59.1	2460	Room temperature	7	WT	potassium phosphate buffer	Table
119	22,387,379	AzoA	Enterococcus faecalis	Methyl Red	null	20.8	null	Room temperature	7	E16G	potassium phosphate buffer	Table
120	22,387,379	AzoA	Enterococcus faecalis	Methyl Red	0.0167	3.17	190	Room temperature	7	R18G	potassium phosphate buffer	Table
121	22,387,379	AzoA	Enterococcus faecalis	Methyl Red	0.0126	140	11110	Room temperature	7	R21G	potassium phosphate buffer	Table
122	22,387,379	AzoA	Enterococcus faecalis	Methyl Red	0.0288	6.61	230	Room temperature	7	L59G	potassium phosphate buffer	Table
123	22,387,379	AzoA	Enterococcus faecalis	Methyl Red	0.0335	21.1	630	Room temperature	7	W62A	potassium phosphate buffer	Table
124	22,387,379	AzoA	Enterococcus faecalis	Methyl Red	0.0053	77.3	14580	Room temperature	7	R66A	potassium phosphate buffer	Table

1

11,900,558

BglB

Pyrococcus horikoshii

pNP-Glc

13.5

34.4

2.58

90°C

4.75

WT

0.1 M Sodium Citrate/0.1 M NaPi

Table

2

11,900,558

BglB

Pyrococcus horikoshii

pNP-Gal

7.3

13.5

1.9

90°C

4.75

WT

0.1 M Sodium Citrate/0.1 M NaPi

Table

3

11,900,558

BglB

Pyrococcus horikoshii

pNP-Man

0.44

4.2

9.8

90°C

4.75

WT

0.1 M Sodium Citrate/0.1 M NaPi

Table

4

11,900,558

BglB

Pyrococcus horikoshii

pNP-Glc

16.2

25.5

1.6

90°C

4.75

Q77R

0.1 M Sodium Citrate/0.1 M NaPi

Table

5

11,900,558

BglB

Pyrococcus horikoshii

pNP-Gal

16.8

14.2

0.85

90°C

4.75

Q77R

0.1 M Sodium Citrate/0.1 M NaPi

Table

6

11,900,558

BglB

Pyrococcus horikoshii

pNP-Man

1.1

1.6

1.4

90°C

4.75

Q77R

0.1 M Sodium Citrate/0.1 M NaPi

Table

7

11,900,558

BglB

Pyrococcus horikoshii

pNP-Glc

0.3

252

870

90°C

4.75

D206N

0.1 M Sodium Citrate/0.1 M NaPi

Table

8

11,900,558

BglB

Pyrococcus horikoshii

pNP-Gal

7.8

571

75.8

90°C

4.75

D206N

0.1 M Sodium Citrate/0.1 M NaPi

Table

9

11,900,558

BglB

Pyrococcus horikoshii

pNP-Man

3.2

45

14.7

90°C

4.75

D206N

0.1 M Sodium Citrate/0.1 M NaPi

Table

10

11,900,558

BglB

Pyrococcus horikoshii

pNP-Glc

30.4

439

14.4

90°C

4.75

Q77R/D206N

0.1 M Sodium Citrate/0.1 M NaPi

Table

11

11,900,558

BglB

Pyrococcus horikoshii

pNP-Gal

36.1

66.5

1.8

90°C

4.75

Q77R/D206N

0.1 M Sodium Citrate/0.1 M NaPi

Table

12

11,900,558

BglB

Pyrococcus horikoshii

pNP-Man

0.68

6.8

9.9

90°C

4.75

Q77R/D206N

0.1 M Sodium Citrate/0.1 M NaPi

Table

13

11,900,558

CelB

Pyrococcus furiosus

pNP-Glc

0.19

1140

7337

90°C

4.75

WT

0.1 M Sodium Citrate/0.1 M NaPi

Table

14

11,900,558

CelB

Pyrococcus furiosus

pNP-Gal

5

2827

561

90°C

4.75

WT

0.1 M Sodium Citrate/0.1 M NaPi

Table

15

11,900,558

CelB

Pyrococcus furiosus

pNP-Man

1.3

65.9

49.8

90°C

4.75

WT

0.1 M Sodium Citrate/0.1 M NaPi

Table

16

11,900,558

CelB

Pyrococcus furiosus

pNP-Glc

13.8

207

14.9

90°C

4.75

R77Q

0.1 M Sodium Citrate/0.1 M NaPi

Table

17

11,900,558

CelB

Pyrococcus furiosus

pNP-Gal

21.7

69.2

3.2

90°C

4.75

R77Q

0.1 M Sodium Citrate/0.1 M NaPi

Table

18

11,900,558

CelB

Pyrococcus furiosus

pNP-Man I

0.64

1.22

1.9

90°C

4.75

R77Q

0.1 M Sodium Citrate/0.1 M NaPi

Table

19

11,900,558

CelB

Pyrococcus furiosus

pNP-Man II

0.15

0.85

5.7

90°C

4.75

R77Q

0.1 M Sodium Citrate/0.1 M NaPi

Table

20

11,900,558

CelB

Pyrococcus furiosus

pNP-Gal

15.7

180

11.5

90°C

4.75

N206D

0.1 M Sodium Citrate/0.1 M NaPi

Table

21

11,900,558

CelB

Pyrococcus furiosus

pNP-Glc

4.6

116

24.9

90°C

4.75

N206D

0.1 M Sodium Citrate/0.1 M NaPi

Table

22

11,900,558

CelB

Pyrococcus furiosus

pNP-Man

0.63

1.97

3.1

90°C

4.75

N206D

0.1 M Sodium Citrate/0.1 M NaPi

Table

23

11,900,558

CelB

Pyrococcus furiosus

pNP-Glc

13.9

172

12.3

90°C

4.75

R77Q/N206D

0.1 M Sodium Citrate/0.1 M NaPi

Table

24

11,900,558

CelB

Pyrococcus furiosus

pNP-Gal

22

29.2

1.3

90°C

4.75

R77Q/N206D

0.1 M Sodium Citrate/0.1 M NaPi

Table

25

11,900,558

CelB

Pyrococcus furiosus

pNP-Man I

1.5

1.9

1.3

90°C

4.75

R77Q/N206D

0.1 M Sodium Citrate/0.1 M NaPi

Table

26

11,900,558

CelB

Pyrococcus furiosus

pNP-Man II

0.1

1.02

10.2

90°C

4.75

R77Q/N206D

0.1 M Sodium Citrate/0.1 M NaPi

Table

27

11,900,558

CelB

Pyrococcus furiosus

pNP-Glc

12

309

26

90°C

4.75

N206S

0.1 M Sodium Citrate/0.1 M NaPi

Table

28

11,900,558

CelB

Pyrococcus furiosus

pNP-Gal

200

1090

5.5

90°C

4.75

N206S

0.1 M Sodium Citrate/0.1 M NaPi

Table

29

11,900,558

CelB

Pyrococcus furiosus

pNP-Man

4.7

15

3.2

90°C

4.75

N206S

0.1 M Sodium Citrate/0.1 M NaPi

Table

30

19,004,819

SDH

S. novella

sulfite

0.0006

63.5

106000

25°C

6.0

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

31

19,004,819

SDH

S. novella

sulfite

0.0011

86.2

75500

25°C

6.5

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

32

19,004,819

SDH

S. novella

sulfite

0.0037

158.8

42700

25°C

7.0

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

33

19,004,819

SDH

S. novella

sulfite

0.0071

293.4

40300

25°C

7.5

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

34

19,004,819

SDH

S. novella

sulfite

0.022

345.3

15300

25°C

8.0

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

35

19,004,819

SDH

S. novella

sulfite

0.086

410

4880

25°C

8.5

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

36

19,004,819

SDH

S. novella

sulfite

0.324

519

1500

25°C

9.0

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

37

19,004,819

SDH

S. novella

sulfite

1.66

431

251

25°C

9.5

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

38

19,004,819

SDH

S. novella

sulfite

3.389

23.7

6.77

25°C

10.0

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

39

19,004,819

SDH

S. novella

sulfite

0.004

36.8

8420

25°C

6.0

Y236F

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

40

19,004,819

SDH

S. novella

sulfite

0.007

41.6

5600

25°C

6.5

Y236F

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

41

19,004,819

SDH

S. novella

sulfite

0.026

51.8

1970

25°C

7.0

Y236F

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

42

19,004,819

SDH

S. novella

sulfite

0.042

48.3

1140

25°C

7.5

Y236F

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

43

19,004,819

SDH

S. novella

sulfite

0.114

53.4

461

25°C

8.0

Y236F

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

44

19,004,819

SDH

S. novella

sulfite

0.332

58.3

173

25°C

8.5

Y236F

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

45

19,004,819

SDH

S. novella

sulfite

1.155

64

54.5

25°C

9.0

Y236F

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

46

19,004,819

SDH

S. novella

sulfite

4.456

52.7

11.6

25°C

9.5

Y236F

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

47

19,004,819

SDH

S. novella

sulfite

15.487

44.8

2.84

25°C

10.0

Y236F

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

48

19,004,819

SDH

S. novella

sulfite

0.812

70.5

86.8

25°C

5.8

R55M

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

49

19,004,819

SDH

S. novella

sulfite

1.087

64

58.9

25°C

6.2

R55M

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

50

19,004,819

SDH

S. novella

sulfite

1.63

64

39.3

25°C

6.6

R55M

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

51

19,004,819

SDH

S. novella

sulfite

1.95

66.3

34

25°C

7.0

R55M

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

52

19,004,819

SDH

S. novella

sulfite

3.62

68.3

18.9

25°C

7.5

R55M

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

53

19,004,819

SDH

S. novella

sulfite

8.17

73.4

8.98

25°C

7.9

R55M

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

54

19,004,819

SDH

S. novella

sulfite

33.27

109.88

3.3

25°C

8.3

R55M

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

55

19,004,819

SDH

S. novella

sulfite

0.667

238.8

358

25°C

6.0

H57A

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

56

19,004,819

SDH

S. novella

sulfite

0.29

226.5

782

25°C

6.5

H57A

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

57

19,004,819

SDH

S. novella

sulfite

0.189

214.5

1130

25°C

7.0

H57A

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

58

19,004,819

SDH

S. novella

sulfite

0.22

220.5

1010

25°C

7.5

H57A

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

59

19,004,819

SDH

S. novella

sulfite

0.27

214.6

799

25°C

8.0

H57A

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

60

19,004,819

SDH

S. novella

sulfite

0.452

222.4

492

25°C

8.5

H57A

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

61

19,004,819

SDH

S. novella

sulfite

1.46

192.5

132

25°C

9.0

H57A

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

62

19,004,819

SDH

S. novella

sulfite

12.2

148.4

12.2

25°C

9.5

H57A

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

63

19,004,819

SDH

S. novella

sulfite

0.0032

730

225000

10°C

6

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

64

19,004,819

SDH

S. novella

sulfite

0.0036

847

239000

10°C

6.5

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

65

19,004,819

SDH

S. novella

sulfite

0.0014

782

55800

10°C

7

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

66

19,004,819

SDH

S. novella

sulfite

0.0031

677

21800

10°C

7.5

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

67

19,004,819

SDH

S. novella

sulfite

0.0087

776

8890

10°C

8

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

68

19,004,819

SDH

S. novella

sulfite

0.412

829

2010

10°C

8.5

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

69

19,004,819

SDH

S. novella

sulfite

1.183

731

618

10°C

9

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

70

19,004,819

SDH

S. novella

sulfite

3.811

674

177

10°C

9.5

WT

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

71

19,004,819

SDH

S. novella

sulfite

4.53

746

165

10°C

5.5

R55M

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

72

19,004,819

SDH

S. novella

sulfite

6.48

662

102

10°C

6

R55M

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

73

19,004,819

SDH

S. novella

sulfite

22.8

240

10.5

10°C

7

R55M

20 mM Tris acetate buffer，0.04 mM cytochrome c，sulfite dehydrogenase mM cytochrome c

Table

98

21,105,905

TCME1

Trypanosoma cruzi

L-Malate

0.13

518

3981

null

7.4

WT

NADP+(0.5), Mn2+(0.5)

Table

99

21,105,905

TCME1

Trypanosoma cruzi

NADP+

0.027

504

18681

null

7.4

WT

L-Malate (5), Mn2+(0.5)

Table

100

21,105,905

TCME1

Trypanosoma cruzi

Pyruvate

7.6

8.3

1.1

null

7.4

WT

NADPH(0.5), Mn2+(0.5), HCO3-(5)

Table

101

21,105,905

TCME1

Trypanosoma cruzi

Mg2+

0.04

461

11513

null

7.4

WT

L-Malate (5), NADP+(0.5)

Table

102

21,105,905

TCME1

Trypanosoma cruzi

Mn2+

0.0043

518

120361

null

7.4

WT

L-Malate (5), NADP+(0.5)

Table

103

21,105,905

TcME2

Trypanosoma cruzi

L-Malate

2.3

683

297

null

7.4

WT

NADP+(0.5), Mn2+(0.5)

Table

104

21,105,905

TcME2

Trypanosoma cruzi

NADP+

0.032

691

21599

null

7.4

WT

L-Malate (35), Mn2+(0.5)

Table

105

21,105,905

TcME2

Trypanosoma cruzi

Pyruvate

5.1

39

8

null

7.4

WT

NADPH(0.5), Mn2+(0.5), HCO3-(5)

Table

106

21,105,905

TcME2

Trypanosoma cruzi

Mg2+

0.57

864

1516

null

7.4

WT

L-Malate (35), NADP+(0.5)

Table

107

21,105,905

TcME2

Trypanosoma cruzi

Mn2+

0.0091

687

75479

null

7.4

WT

L-Malate (35), NADP+(0.5)

Table

108

21,105,905

TbME1

Trypanosoma brucei

L-Malate

0.32

1132

3536

null

7.4

WT

NADP+(0.5), Mn2+(0.5)

Table

109

21,105,905

TbME1

Trypanosoma brucei

NADP+

0.048

1206

25166

null

7.4

WT

L-Malate (5), Mn2+(0.5)

Table

110

21,105,905

TbME1

Trypanosoma brucei

Pyruvate

4.3

113

26

null

7.4

WT

NADPH(0.5), Mn2+(0.5), HCO3-(5)

Table

111

21,105,905

TbME1

Trypanosoma brucei

Mg2+

0.27

1280

4739

null

7.4

WT

L-Malate (5), NADP+(0.5)

Table

112

21,105,905

TbME1

Trypanosoma brucei

Mn2+

0.0024

905

377192

null

7.4

WT

L-Malate (5), NADP+(0.5)

Table

113

21,105,905

TbME2

Trypanosoma brucei

L-Malate

0.49

242

494

null

7.4

WT

NADP+(0.5), Mn2+(0.5)

Table

114

21,105,905

TbME2

Trypanosoma brucei

NADP+

0.016

284

17775

null

7.4

WT

L-Malate (5), Mn2+(0.5)

Table

115

21,105,905

TbME2

Trypanosoma brucei

Pyruvate

5.3

34

6

null

7.4

WT

NADPH(0.5), Mn2+(0.5), HCO3-(5)

Table

116

21,105,905

TbME2

Trypanosoma brucei

Mg2+

0.58

208

359

null

7.4

WT

L-Malate (5), NADP+(0.5)

Table

117

21,105,905

TbME2

Trypanosoma brucei

Mn2+

0.0066

246

37302

null

7.4

WT

L-Malate (5), NADP+(0.5)

Table

118

22,387,379

AzoA

Enterococcus faecalis

Methyl Red

0.024

59.1

2460

Room temperature

7

WT

potassium phosphate buffer

Table

119

22,387,379

AzoA

Enterococcus faecalis

Methyl Red

null

20.8

null

Room temperature

7

E16G

potassium phosphate buffer

Table

120

22,387,379

AzoA

Enterococcus faecalis

Methyl Red

0.0167

3.17

190

Room temperature

7

R18G

potassium phosphate buffer

Table

121

22,387,379

AzoA

Enterococcus faecalis

Methyl Red

0.0126

140

11110

Room temperature

7

R21G

potassium phosphate buffer

Table

122

22,387,379

AzoA

Enterococcus faecalis

Methyl Red

0.0288

6.61

230

Room temperature

7

L59G

potassium phosphate buffer

Table

123

22,387,379

AzoA

Enterococcus faecalis

Methyl Red

0.0335

21.1

630

Room temperature

7

W62A

potassium phosphate buffer

Table

124

22,387,379

AzoA

Enterococcus faecalis

Methyl Red

0.0053

77.3

14580

Room temperature

7

R66A

potassium phosphate buffer

Table