Comparison between dual and tri-colour reagents for the analysis of lymphocyte subsets. 
The aim of this project was to compare dual and tri-colour reagents for lymphocyte immunophenotyping.  A total of 37 patient and normal specimens were immunophenotyped concurrently with the following mean values (% dual vs tri-colour): CD3 (69.4 vs 68.3) CD4 (24.0 vs 24.2) and CD19 (13.9 vs 12.6).  A comparison of the results obtained using the paired t test showed that there were no significant differences for cells expressing CD3, CD4 and CD19.  However, there was a significant difference in the NK (18.3 vs 16.3) cell component.  A major advantage in using 3 colour immunophenotyping is the ability to analyse specimens that cannot be analysed using dual colour reagents due to debris or contamination of the gate with non-lymphocytic cells.