Electrophoresis of proteins and nucleic acids on acrylamide-agarose gels lacking covalent crosslinking. 
The preparation of acrylamide-agarose gels lacking covalent crosslinking with methylenebisacrylamide is described.  These hybrid gels melt at 85 degrees C and, consequently, allow quantitative analysis of tritium-labeled protein after electrophoresis.  Recovery of tritium-labeled ribonucleic acids extracted from hybrid gels is 20 to 25% greater than from standard acrylamide-methylenebisacrylamide gels.  Standard curves of electrophoretic mobilities as a function of molecular weights of dissociated proteins and ribonucleic acids are compared for acrylamide-agarose gels and acrylamide-methylenebisacrylamide gels.