Cytokine-induced neutrophil chemotaxis assay. 
Chemotaxis is directed migration of a cell type to a distant chemoattractant.  When this chemoattractant is a cytokine, the term chemokine is often used.  Chemotaxis by neutrophils, specifically polymorphonuclear leukocytes (PMNs), plays a critical role in the innate immune response.  On an equimolar basis, interleukin-8 (IL-8) is one of the most potent PMN chemokines known.  This chapter describes an in vitro chemotaxis technique using PMNs and IL-8 which can serve the investigator as an established model from which new studies can be developed.