Comparative Protein Profiling of Intraphagosomal Expressed Proteins of Mycobacterium bovis BCG. 
BCG, the only available vaccine against tuberculosis affords a variable protection which wanes with time.  In this study we have analyzed and compared the proteins which are expressed differentially during broth-culture and intraphagosomal growth of M.bovis BCG.  Eight proteins which showed increased expression during the intraphagosomal growth were identified by MALDI-TOF/MS.  These were - a precursor of alanine and proline-rich secreted protein apa, isoforms of malate dehydrogenase, large subunit alpha (Alpha-ETF) of electron transfer flavoprotein, immunogenic protein MPB64 precursor, UPF0036 protein, and two proteins with unknown function.  Based on these findings we speculate that higher expression of these proteins has a probable role in intracellular survival, adaptation and/or immunoprotective effect of BCG.  Further, these proteins might also be used as gene expression markers for endosome trafficking events of BCG.