Effects of estrogenic oral contraceptives on the lipoprotein B particle system defined by apolipoproteins E and C-III content. 
Apolipoproteins E and C-III are modulators of lipoprotein metabolism that could affect development of atherosclerosis.  The prevalence in plasma of apoB-containing particles (LpB) that contain either apoE or apoC-III, both or neither, and the effect of estrogen on these lipoproteins are unknown.  The LpB particle system, defined by the presence or absence of apoE or C-III, was studied in 13 normolipidemic women, 7 nonusers and 6 users of oral contraceptives.  Fasting plasma was separated by anti-apoE and C-III affinity chromatography and ultracentrifugation into four types of VLDL, IDL, and LDL particles: with apoE but not apoC-III (E+C-), apoC-III but not apoE (E-C+), both (E+C+) or neither (E-C-).  The predominant VLDL particles were E-C- (42% in nonusers, 56% in users) and E+C+ (39% in nonusers, 24% in users), suggesting that apoE and apoC-III mainly exist together in VLDL.  In IDL, E-C- was the major fraction (74% nonusers, 81% users), and in LDL, it was 99% in both groups.  The triglycerides in VLDL and IDL were mainly contained in C+ particles (79% and 66% of the total VLDL and IDL triglycerides, respectively).  Within VLDL, IDL, and LDL, E-C- particles had the smallest size and E+C+ or E-C+ the largest.  Users had higher concentrations of VLDL E-C- (280%) and IDL E-C- (90%) particles than nonusers.  They also had higher free cholesterol and cholesteryl ester concentrations associated with these fractions and with VLDL E-C+.  The triglyceride contents of VLDL E-C- particles were lower in users of oral contraceptives than in nonusers.  This study demonstrates that the elevated VLDL TG concentrations in users of estrogen-dominant oral contraceptives is mainly caused by an increased concentration of small VLDL particles that have reduced TG content, and that do not have apoE and C-III.  These particles may have lower atherogenicity than particles enriched with apoE and C-III.-Khoo, C., H. Campos, H. Judge, and F. M. Sacks.  Effects of estrogenic oral contraceptives on the lipoprotein B particle system defined by apolipoproteins E and C-III content.