Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice. 
Notch2 and Delta-like 1 (Dll1) have been implicated in the development of marginal zone B (MZB) cells.  In the present study, we characterized the expression and function of mouse Notch receptors and ligands in the spleen by using newly generated mAbs.  Although Notch2 was expressed on both B and T cells in the spleen, the highest expression was observed on precursors of marginal zone B and MZB cells.  Dll1 was expressed on macrophage and erythroblasts in the red pulp, but not on B cells or marginal zone macrophage.  Administration of a blocking mAb against Dll1 not only blocked the development of MZB cells in juvenile mice but also gradually depleted the pre-established MZB cells in adult mice, indicating a critical role for Dll1 in the maintenance of MZB cells in the spleen of normal mice.  Interestingly, Dll1 was not necessary for the maintenance of MZB cells in lupus-prone (NZB x NZW) F1 mice particularly after the onset of the disease, suggesting that the Dll1 independence may be a feature of dysregulated MZB cells producing auto-antibodies.