Characterization of the discriminative stimulus properties induced by 5-HT1 and 5-HT2 agonists in rats. 
The effect of different serotonin (5-HT) agonists and antagonists on the discriminative stimulus properties (cue) induced by 8-hydroxy-2-(di-n-propylamino)-tetralin (8-OHDPAT), 1-(m-trifluoromethylphenyl)piperazine (TFMPP) and d-LSD (d-lysergic acid diethylamide) has been investigated.  The 8-OHDPAT cue was mimicked by the 5-HT1A agonists ipsapirone, buspirone, gepirone and partially by 5-methoxy-N,N-dimethyltryptamine and d-LSD.  5-HT1B (TFMPP and RU 24969) and 5-HT2 agonists (DOM, DOI and quipazine) were ineffective and induced disruption of responding.  The 8-OHDPAT cue was antagonized by spiroxatrine and partially by (-)-alprenolol, whereas selective antagonists of 5-HT2 (ketanserin and ritanserin), 5-HT3 (ICS 205-930), alpha 1-adrenergic (prazosin) and beta-adrenergic receptors (ICI 118.551) were ineffective.  The TFMPP cue was mimicked by RU 24969 and partially by quipazine.  Other compounds were ineffective.  Only (-)-alprenolol antagonized the effect of TFMPP.  The d-LSD cue was mimicked by DOM, DOI, quipazine, 5-methoxy-N,N-dimethyltryptamine and partially by ipsapirone, TFMPP and RU 24969.  The 3 latter compounds and 5-HT1A agonists induced disruption of responding.  The d-LSD cue was antagonized by ketanserin and ritanserin, but not by the other antagonists mentioned above.  The specific inhibitor of 5-HT uptake citalopram was not able to substitute for any of the 3 agonists.  It is concluded that the drug discrimination technique can be used to identify selective agonists and antagonists of 5-HT receptor subtypes.  Compounds with mixed effects on 5-HT receptor subtypes can also be identified.  These show additional effects on reaction time and often disrupt responding at higher dosages.