Gender differences and lateralization in the distribution pattern of insulin-like growth factor-1 receptor in developing rat hippocampus: an immunohistochemical study. 
Numerous investigators have provided data supporting essential roles for insulin-like growth factor-I (IGF-I) in development of the brain.  The aim of this study was to immunohistochemically determine the distinct regional distribution pattern of IGF-1 receptor (IGF-IR) expression in various portions of newborn rat hippocampus on postnatal days 0 (P0), 7 (P7), and 14 (P14), with comparison between male/female and right/left hippocampi.  We found an overall significant increase in distribution of IGF-IR-positive (IGF-IR+) cells in CA1 from P0 until P14.  Although, no marked changes in distribution of IGF-IR+ cells in areas CA2 and CA3 were observed; IGF-IR+ cells in DG decreased until P14.  The smallest number of immunoreactive cells was present in CA2 and the highest number in DG at P0.  Moreover, in CA1, CA3, and DG, the number of IGF-IR+ cells was markedly higher in both sides of the hippocampus in females.  Our data also showed a higher mean number of IGF-IR+ cells in the left hippocampus of female at P7.  By contrast, male pups showed a significantly higher number of IGF-IR+ cells in the DG of the right hippocampus.  At P14, the mean number of immunoreactive cells in CA1, CA3, and DG areas found to be significantly increased in left side of hippocampus of males, compared to females.  These results indicate the existence of a differential distribution pattern of IGF-IR between left-right and male-female hippocampi.  Together with other mechanisms, these differences may underlie sexual dimorphism and left-right asymmetry in the hippocampus.