Two-dimensional gel-based alkaline proteome of the probiotic bacterium Lactobacillus acidophilus NCFM. 
Lactobacillus acidophilus NCFM (NCFM) is a well-documented probiotic bacterium isolated from human gut.  Detailed 2D gel-based NCFM proteomics addressed the so-called alkaline range, i.e., pH 6-11.  Proteins were identified in 150 of the 202 spots picked from the Coomassie Brilliant Blue stained 2D gel using MALDI-TOF-MS.  The 102 unique gene products among the 150 protein identifications were assigned to different functional categories, and evaluated by considering a calculated distribution of abundance as well as grand average of hydrophobicity values.  None of the very few available lactic acid bacteria proteome reference maps included the range of pI >7.0.  The present report of such data on the proteome of NCFM fundamentally complements current knowledge on protein profiles limited to the acid and neutral pH range.