Background of CD111 / Nectin 1 antibody

Nectins, which were origilly identified as virus receptors, are members of the cell-cell adhesion molecule (CAM) family.  They are Ca2+-independent immunoglobulin-like CAMs.  The nectin family comprises four members, nectin-1, nectin-2, nectin-3 and nectin-4, which are encoded by the PVRL1, PVRL2, PVRL3 and PVRL4 genes, respectively.  In the central nervous system, these cell adhesion molecules aggregate in formations, termed puncta adherentia junctions, which are mechanical adhesive sites that connect pre- and postsyptic membranes.  All nectins contain an extracellular region with three immunoglobulin-like loops (one V type and two C2 types), a single membrane-spanning region and a cytoplasmic tail.  Nectins directly bind afadin, an F-actin-binding protein, through their cytoplasmic tails.  Nectin-1 is expressed at cell-cell junctions in human and mouse epidermis.  In mice lacking the gene encoding nectin-1 (Pvrl1-/- mice), the expression of loricrin, a differentiation marker and a major component of cornified cell envelopes in the epidermis, is downregulated and newborn pups have a shiny and slightly reddish skin.  Mutations in human PVRL1 are implicated in cleft lip or palate-ectodermal dysplasia syndromes, which includes Zlotogora–Ogur syndrome and Margarita Island ectodermal dysplasia.  Both of these are autosomal recessive disorders that are clinically characterized by unusual facial appearance, dental anomalies, hypotrichosis, palmoplantar hyperkeratosis and onychodysplasia, syndactyly, cleft lip or palate, and in some cases, mental retardation. 

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PVRL1 (RC211214, Right lane) cDNA for 48 hrs and lysed.  Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PVRL1. 

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PVRL1 (RC211214, Right lane) cDNA for 48 hrs and lysed.  Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PVRL1. 

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PVRL1 (RC211214, Right lane) cDNA for 48 hrs and lysed.  Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PVRL1. 

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PVRL1 (RC211214, Right lane) cDNA for 48 hrs and lysed.  Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PVRL1. 

Predicted cell location: Cytoplasm.  Positive control: Human stomach cancer tissue.  Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human stomach cancer tissue using PVRL1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. ( Original magnification:x200)