Lipopolysaccharide induces raft domain expansion in membrane composed of a phospholipid-cholesterol-sphingomyelin ternary system. 
The molecular behavior and interaction of Re-type lipopolysaccharide (ReLPS) and phospholipids were investigated in two different types of model membrane systems, a pure phospholipid membrane consisting of 1,2-dielaidoyl-snglycero-3-phosphoethanolamine (DEPE) and a raft-forming membrane composed of equimolar DEPE, sphingomyelin (SM), and cholesterol (Chol) by solid-state NMR spectroscopy.  A remarkable influence of ReLPS on the property of lipid bilayer was found by analyzing the (13)C-NMR spectra.  Namely, while both liquid-ordered (L(o)) and liquid-disordered (L(d)) phases co-exist in DEPE/SM/Chol, only the L(o) phase is present in DEPE/SM/Chol/ReLPS.  This clearly indicates that ReLPS induces expansion of the raft area in the raft-forming membrane.  The (1)H spin-lattice relaxation times in the rotating frame T( 1ρ) (H) in the two different membranes, DEPE/ReLPS and DEPE/SM/Chol/ReLPS, indicate that the motion of DEPE is affected by the presence of ReLPS, Chol, and SM, and much faster than that of ReLPS in both membranes.  The ReLPS in the raft-forming membrane, in particular, accelerated the movement of DEPE.  Thus, this study shows the possibility that LPS induces the expansion of raft region and the rapid motion of the raft-forming membranes to favor molecular interactions in the animal cell membrane during innate immune recognition.