Requirement of B lymphocytes in local adoptive transfer of experimental allergic orchitis (EAO) by lymph node cells. 
The necessity of T lymphocytes in local adoptive transfer of experimental allergic orchitis (EAO) is an established fact.  The present study was undertaken to elucidate the participation of B lymphocytes in the local transfer system of EAO.  The capacity of purified lymph node T and B cells to transfer EAO was compared.  Donor strain 13 guinea pigs were sensitized with testicular antigen and complete Freund's adjuvant.  We then injected 1 X 10(8) cells just under the tunica albuginea of the testis of a syngeneic recipient.  The recipients were killed 5 days after cell transfer.  Nylon-wool nonadherent T cells alone produced only minimal lesions, but could induce more severe mononuclear lesions with the addition of syngeneic, normal macrophages.  In contrast, B-cell enriched populations either alone or combined with a small number of macrophages, were able to transfer extensive lesions.  In the combined transfer of immune T and immune B cells the severity of transferred lesions was dependent upon the number of B cells.  These results suggest that cooperative interactions among T cells, B cells, and macrophages are involved in the generation of transferred testicular lesions, with emphasis on the participation of B cells.