[Lipase and phospholipase from Staphylococcus aureus of different origin.  II.  Purification and characterization (author's transl)]. 
Lipase and phospholipase C from Staphylococcus aureus could be isolated by gel filtration on Sephacryl S 200 (Fig.  1a, b) and completely separated by refiltration under the same conditions.  Isoelectric focusing gave maximal enzyme-activities for lipase at pH 8.6 and 9.5 and for phospholipase C at pH 7.4 (Fig.  2).  Thin-layer chromatography revealed that the reaction products in lecithin agar of the phospholipase C-preparations from S. aureus and Bacillus cereus were identical (Table 1).