id
stringlengths
1
5
tokens
sequence
ner_tags
sequence
16400
[ "To", "understand", "the", "role", "of", "MARs", "in", "IgH", "enhancer", "regulation", ",", "we", "have", "identified", "a", "novel", "MAR-binding", "protein", ",", "MAR-BP1", ",", "from", "soluble", "nuclear", "matrix", "preparations", "based", "on", "its", "ability", "to", "bind", "to", "the", "MARs", "associated", "with", "the", "IgH", "enhancer", "." ]
[ 0, 0, 0, 0, 0, 15, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 7, 8, 0 ]
16401
[ "Purified", "MAR-BP1", "migrates", "as", "a", "33-kDa", "protein", ",", "and", "it", "can", "be", "found", "in", "nuclear", "matrix", "preparations", "from", "a", "number", "of", "different", "types", "of", "lymphoid", "cell", "lines", "." ]
[ 0, 15, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 11, 12, 12, 0 ]
16402
[ "Although", "specific", "binding", "sites", "have", "been", "difficult", "to", "localize", "by", "chemical", "or", "enzymatic", "footprinting", "procedures", ",", "NF-muNR", "binding", "sites", "are", "critical", "for", "efficient", "MAR-BP1", "binding", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 0, 0, 15, 0, 0 ]
16403
[ "Indeed", ",", "binding", "of", "the", "IgH", "enhancer", "to", "either", "intact", "nuclear", "matrix", "preparations", "or", "to", "MAR-BP1", "is", "mutually", "exclusive", "to", "NF-muNR", "binding", "." ]
[ 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 15, 0, 0 ]
16404
[ "These", "results", "are", "consistent", "with", "a", "model", "for", "cell-type", "specific", "regulation", "in", "which", "binding", "of", "the", "NF-muNR", "repressor", "to", "the", "IgH", "enhancer", "prevents", "nuclear", "matrix", "attachment", "in", "inappropriate", "cells", "by", "interfering", "with", "MAR-BP1", "/enhancer", "interaction", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 7, 0, 0 ]
16405
[ "PU.1", "(", "Spi-1", ")", "and", "C/EBP", "alpha", "regulate", "expression", "of", "the", "granulocyte-macrophage", "colony-stimulating", "factor", "receptor", "alpha", "gene", "." ]
[ 15, 0, 15, 0, 0, 15, 16, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 0 ]
16406
[ "Growth", "factor", "receptors", "play", "an", "important", "role", "in", "hematopoiesis", "." ]
[ 15, 16, 16, 0, 0, 0, 0, 0, 0, 0 ]
16407
[ "In", "order", "to", "further", "understand", "the", "mechanisms", "directing", "the", "expression", "of", "these", "key", "regulators", "of", "hematopoiesis", ",", "we", "initiated", "a", "study", "investigating", "the", "transcription", "factors", "activating", "the", "expression", "of", "the", "granulocyte-macrophage", "colony-stimulating", "factor", "(", "GM-CSF", ")", "receptor", "alpha", "gene", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 8, 8, 8, 0 ]
16408
[ "Here", ",", "we", "demonstrate", "that", "the", "human", "GM-CSF", "receptor", "alpha", "promoter", "directs", "reporter", "gene", "activity", "in", "a", "tissue-specific", "fashion", "in", "myelomonocytic", "cells", ",", "which", "correlates", "with", "its", "expression", "pattern", "as", "analyzed", "by", "reverse", "transcription", "PCR", "." ]
[ 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0, 7, 8, 0, 0, 0, 0, 0, 0, 13, 14, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16409
[ "The", "GM-CSF", "receptor", "alpha", "promoter", "contains", "an", "important", "functional", "site", "between", "positions", "-53", "and", "-41", "as", "identified", "by", "deletion", "analysis", "of", "reporter", "constructs", "." ]
[ 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]
16410
[ "We", "show", "that", "the", "myeloid", "and", "B", "cell", "transcription", "factor", "PU.1", "binds", "specifically", "to", "this", "site", "." ]
[ 0, 0, 0, 0, 0, 0, 15, 16, 16, 16, 15, 0, 0, 0, 0, 0, 0 ]
16411
[ "Furthermore", ",", "we", "demonstrate", "that", "a", "CCAAT", "site", "located", "upstream", "of", "the", "PU.1", "site", "between", "positions", "-70", "and", "-54", "is", "involved", "in", "positive-negative", "regulation", "of", "the", "GM-CSF", "receptor", "alpha", "promoter", "activity", "." ]
[ 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0 ]
16412
[ "C/EBP", "alpha", "is", "the", "major", "CCAAT/enhancer-binding", "protein", "(", "C/EBP", ")", "form", "binding", "to", "this", "site", "in", "nuclear", "extracts", "of", "U937", "cells", "." ]
[ 15, 16, 0, 0, 0, 15, 16, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 11, 12, 0 ]
16413
[ "Point", "mutations", "of", "either", "the", "PU.1", "site", "or", "the", "C/EBP", "site", "that", "abolish", "the", "binding", "of", "the", "respective", "factors", "result", "in", "a", "significant", "decrease", "of", "GM-CSF", "receptor", "alpha", "promoter", "activity", "in", "myelomonocytic", "cells", "only", "." ]
[ 0, 0, 0, 0, 0, 7, 8, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 13, 14, 0, 0 ]
16414
[ "Furthermore", ",", "we", "demonstrate", "that", "in", "myeloid", "and", "B", "cell", "extracts", ",", "PU.1", "forms", "a", "novel", ",", "specific", ",", "more", "slowly", "migrating", "complex", "(", "PU-SF", ")", "when", "binding", "the", "GM-CSF", "receptor", "alpha", "promoter", "PU.1", "site", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 15, 16, 16, 0, 7, 8, 0 ]
16415
[ "This", "is", "the", "first", "demonstration", "of", "a", "specific", "interaction", "with", "PU.1", "on", "a", "myeloid", "PU.1", "binding", "site", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 7, 8, 8, 8, 0 ]
16416
[ "The", "novel", "complex", "is", "distinct", "from", "that", "described", "previously", "as", "binding", "to", "B", "cell", "enhancer", "sites", "and", "can", "be", "formed", "by", "addition", "of", "PU.1", "to", "extracts", "from", "certain", "nonmyeloid", "cell", "types", "which", "do", "not", "express", "PU.1", ",", "including", "T", "cells", "and", "epithelial", "cells", ",", "but", "not", "from", "erythroid", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 13, 14, 14, 0, 0, 0, 0, 15, 0, 0, 13, 14, 0, 13, 14, 0, 0, 0, 0, 13, 14, 0 ]
16417
[ "Furthermore", ",", "we", "demonstrate", "that", "the", "PU-SF", "complex", "binds", "to", "PU.1", "sites", "found", "on", "a", "number", "of", "myeloid", "promoters", ",", "and", "its", "formation", "requires", "an", "intact", "PU.1", "site", "adjacent", "to", "a", "single-stranded", "region", "." ]
[ 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 15, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0 ]
16418
[ "Expression", "of", "PU.1", "in", "nonmyeloid", "cells", "can", "activate", "the", "GM-CSF", "receptor", "alpha", "promoter", "." ]
[ 0, 0, 15, 0, 13, 14, 0, 0, 0, 7, 8, 8, 8, 0 ]
16419
[ "Deletion", "of", "the", "amino-terminal", "region", "of", "PU.1", "results", "in", "a", "failure", "to", "form", "the", "PU-SF", "complex", "and", "in", "a", "concomitant", "loss", "of", "transactivation", ",", "suggesting", "that", "formation", "of", "the", "PU-SF", "complex", "is", "of", "functional", "importance", "for", "the", "activity", "of", "the", "GM-CSF", "receptor", "alpha", "promoter", "." ]
[ 0, 0, 0, 15, 16, 0, 15, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0 ]
16420
[ "Finally", ",", "we", "demonstrate", "that", "C/EBP", "alpha", "can", "also", "active", "the", "GM-CSF", "receptor", "alpha", "promoter", "in", "nonmyeloid", "cells", "." ]
[ 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 7, 8, 8, 8, 0, 13, 14, 0 ]
16421
[ "(", "ABSTRACT", "TRUNCATED", "AT", "400", "WORDS", ")" ]
[ 0, 0, 0, 0, 0, 0, 0 ]
16422
[ "HMG-I", "binds", "to", "GATA", "motifs", ":", "implications", "for", "an", "HPFH", "syndrome", "." ]
[ 15, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0 ]
16423
[ "We", "have", "examined", "binding", "of", "the", "nuclear", "protein", "HMG-I", "to", "the", "human", "gamma-globin", "promoter", "." ]
[ 0, 0, 0, 0, 0, 0, 15, 16, 15, 0, 0, 7, 8, 8, 0 ]
16424
[ "We", "find", "that", "HMG-I", "binds", "preferentially", "to", "the", "more", "3", "'", "of", "a", "pair", "of", "GATA", "motifs", "in", "the", "gamma-globin", "promoter", ";", "this", "paired", "motif", "is", "bound", "by", "the", "erythroid", "factor", "GATA-1", "." ]
[ 0, 0, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 16, 0 ]
16425
[ "A", "naturally", "occurring", "mutation", "(", "-175", "T-C", ")", "in", "the", "area", "bound", "by", "HMG-I", "results", "in", "overexpression", "of", "gamma-globin", "in", "adult", "red", "blood", "cells", "(", "HPFH", ")", "and", "up-regulation", "of", "the", "gamma-globin", "promoter", "in", "in", "vitro", "expression", "assays", ";", "HMG-I", "does", "not", "bind", "to", "this", "mutant", "sequence", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 15, 0, 13, 14, 14, 14, 0, 13, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 0, 7, 8, 0 ]
16426
[ "A", "survey", "of", "GATA", "motifs", "from", "other", "globin", "cis-elements", "demonstrates", "HMG-I", "binding", "to", "most", "of", "them", "." ]
[ 0, 0, 0, 7, 8, 0, 0, 7, 8, 0, 15, 0, 0, 0, 0, 0, 0 ]
16427
[ "These", "findings", "implicate", "HMG-I", "in", "the", "HPFH", "phenotype", ";", "we", "speculate", "that", "it", "may", "participate", "in", "the", "formation", "of", "multiprotein", "complexes", "that", "regulate", "globin", "gene", "expression", "." ]
[ 0, 0, 0, 15, 0, 0, 13, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0 ]
16428
[ "Constitutive", "activation", "of", "different", "Jak", "tyrosine", "kinases", "in", "human", "T", "cell", "leukemia", "virus", "type", "1", "(", "HTLV-1", ")", "tax", "protein", "or", "virus-transformed", "cells", "." ]
[ 0, 0, 0, 0, 15, 16, 16, 0, 15, 16, 16, 16, 16, 16, 16, 16, 16, 16, 16, 16, 0, 11, 12, 0 ]
16429
[ "HTLV-1", "infection", "causes", "an", "adult", "T", "cell", "leukemia", "in", "humans", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16430
[ "The", "viral", "encoded", "protein", "tax", ",", "is", "thought", "to", "play", "an", "important", "role", "in", "oncogenesis", "." ]
[ 0, 15, 16, 16, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16431
[ "Our", "previous", "data", "obtained", "from", "a", "tax", "transgenic", "mouse", "model", "revealed", "that", "tax", "transforms", "mouse", "fibroblasts", "but", "not", "thymocytes", ",", "despite", "comparable", "levels", "of", "tax", "expression", "in", "both", "tissues", "." ]
[ 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 0, 15, 0, 13, 14, 0, 0, 13, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 0 ]
16432
[ "Constitutive", "tyrosine", "phosphorylation", "of", "a", "130-kD", "protein", "(", "s", ")", "was", "observed", "in", "the", "tax", "transformed", "fibroblast", "B", "line", "and", "in", "HTLV-1", "transformed", "human", "lymphoid", "lines", ",", "but", "not", "in", "thymocytes", "from", "Thy-tax", "transgenic", "mice", "." ]
[ 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0, 15, 11, 12, 12, 12, 0, 0, 11, 12, 12, 12, 12, 0, 0, 0, 0, 13, 0, 0, 0, 0, 0 ]
16433
[ "Phosphotyrosine", "immunoprecipitation", "followed", "by", "Western", "blot", "analysis", "with", "a", "set", "of", "Jak", "kinase", "specific", "antibodies", ",", "identified", "p130", "as", "Jak2", "in", "the", "tax", "transformed", "mouse", "fibroblastic", "cell", "line", "and", "Jak3", "in", "HTLV-1", "transformed", "human", "T", "cell", "lines", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 16, 16, 0, 0, 0, 0, 15, 0, 0, 15, 0, 11, 12, 12, 12, 0, 15, 0, 11, 12, 12, 12, 12, 12, 0 ]
16434
[ "Phosphorylation", "of", "Jak2", "in", "tax", "transformed", "cells", "resulted", "from", "high", "expression", "of", "IL-6", "." ]
[ 0, 0, 15, 0, 11, 12, 12, 0, 0, 0, 0, 0, 0, 0 ]
16435
[ "Tyrosine", "phosphorylation", "of", "this", "protein", "could", "also", "be", "induced", "in", "Balb/c3T3", "cells", "using", "a", "supernatant", "from", "the", "B", "line", ",", "which", "was", "associated", "with", "induction", "of", "cell", "proliferation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 11, 12, 0, 0, 0, 0, 0, 11, 12, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16436
[ "Both", "phosphorylation", "and", "proliferation", "were", "inhibited", "by", "IL-6", "neutralizing", "antibodies", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 15, 16, 16, 0 ]
16437
[ "Constitutive", "phosphorylation", "of", "Jak", "kinases", "may", "facilitate", "tumor", "growth", "in", "both", "HTLV-1", "infected", "human", "T", "cells", "and", "the", "transgenic", "mouse", "model", "." ]
[ 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 13, 14, 14, 14, 14, 0, 0, 0, 0, 0, 0 ]
16438
[ "Regulation", "of", "c-jun", "mRNA", "expression", "by", "hydroxyurea", "in", "human", "K562", "cells", "during", "erythroid", "differentiation", "[", "published", "erratum", "appears", "in", "Biochim", "Biophys", "Acta", "1995", "Dec", "27", ";", "1264", "(", "3", ")", ":", "409", "]" ]
[ 0, 0, 9, 10, 0, 0, 0, 0, 11, 12, 12, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16439
[ "Hydroxyurea", "(", "HU", ")", "is", "an", "antitumor", "agent", "which", "also", "induces", "hemoglobinization", "during", "erythroid", "differentiation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16440
[ "In", "addition", ",", "HU", "stimulates", "the", "synthesis", "of", "fetal", "hemoglobin", "in", "sickle", "cell", "anemia", "patients", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0 ]
16441
[ "To", "further", "understand", "its", "mechanism", "of", "action", ",", "we", "investigated", "the", "effects", "of", "HU", "on", "regulation", "of", "c-jun", "expression", "prior", "to", "the", "onset", "of", "erythroid", "differentiation", "of", "K562", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 11, 12, 0 ]
16442
[ "HU", "induced", "a", "dose-dependent", "stimulation", "of", "c-jun", "synthesis", "." ]
[ 0, 0, 0, 0, 0, 0, 7, 0, 0 ]
16443
[ "The", "levels", "of", "c-jun", "mRNA", "was", "elevated", "4", "to", "7.5-fold", "by", "HU", "within", "2", "h", "." ]
[ 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16444
[ "This", "was", "followed", "by", "a", "gradual", "decline", "to", "the", "basal", "level", "by", "24", "h", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16445
[ "Both", "nuclear", "run-on", "and", "actinomycin", "D", "pulse", "experiments", "strongly", "indicate", "that", "HU", "regulates", "c-jun", "mRNA", "expression", "by", "increasing", "the", "rate", "of", "synthesis", "as", "well", "as", "stabilizing", "the", "c-jun", "mRNA", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]
16446
[ "In", "addition", ",", "the", "level", "of", "jun", "protein", "was", "elevated", "by", "2", "to", "5-fold", "within", "4", "h", "in", "HU", "treated", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 11, 12, 12, 0 ]
16447
[ "Furthermore", ",", "concentrations", "of", "HU", "below", "250", "microM", "slightly", "increased", "the", "5X", "AP-1", "/CAT", "activity", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 15, 0, 0 ]
16448
[ "These", "results", "strongly", "suggest", "that", "HU", "induces", "both", "transcriptional", "and", "post-transcription", "regulation", "of", "c-jun", "during", "erythroid", "differentiation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0 ]
16449
[ "In", "vivo", "and", "in", "vitro", "effects", "of", "glucocorticoids", "on", "lymphocyte", "proliferation", "in", "man", ":", "relationship", "to", "glucocorticoid", "receptors", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0 ]
16450
[ "Interrelations", "between", "the", "hypothalamic-pituitary-adrenal", "system", "(", "HPA", ")", "and", "the", "immune", "system", "represent", "a", "well-documented", "biological", "phenomenon", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16451
[ "While", "in", "vitro", "administration", "of", "glucocorticoids", "may", "inhibit", "concanavalin", "A", "(", "Con", "A", ")", "-", "and", "phytohemagglutinin", "(", "PHA", ")", "-induced", "T-cell", "proliferation", ",", "pokeweed", "mitogen", "(", "PWM", ")", "-driven", "B-cell", "mitogenesis", "is", "relatively", "resistant", "to", "glucocorticoids", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16452
[ "To", "further", "explore", "the", "link", "between", "the", "HPA", "and", "the", "immune", "system", "in", "relation", "to", "glucocorticoid", "receptor", "function", ",", "dose-response", "curves", "were", "obtained", "for", "Con", "A", "-and", "PHA", "-induced", "T-cell", "mitogenesis", ",", "PWM", "-generated", "B-cell", "mitogenesis", "and", "spontaneous", "lymphocyte", "proliferation", "in", "13", "healthy", "controls", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16453
[ "Glucocorticoid", "effects", "were", "assessed", "in", "vivo", "by", "depletion", "of", "endogenous", "glucocorticoids", "after", "oral", "administration", "of", "1.5", "g", "metyrapone", "(", "MET", ")", "and", "subsequent", "glucocorticoid", "replacement", ",", "and", "in", "vitro", "by", "incubation", "of", "the", "cells", "with", "different", "doses", "of", "dexamethasone", "(", "DEX", ")", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16454
[ "There", "was", "a", "significant", "decrease", "in", "PWM", "-induced", "B-cell", "mitogenesis", "and", "a", "more", "pronounced", "effect", "of", "DEX", "administered", "in", "vitro", "on", "spontaneous", "lymphocyte", "proliferation", "after", "MET", "treatment", "when", "compared", "with", "the", "DEX", "plus", "MET", "pretreated", "condition", "in", "vivo", "." ]
[ 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16455
[ "These", "data", "suggest", "that", "the", "inhibition", "of", "spontaneous", "lymphocyte", "proliferation", "by", "glucocorticoids", "in", "vitro", "is", "related", "to", "glucocorticoid", "receptor", "function", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0 ]
16456
[ "The", "decrease", "in", "PWM", "-generated", "B-cell", "proliferation", "following", "cortisol", "depletion", "by", "MET", "may", "be", "seen", "in", "connection", "with", "impaired", "glucocorticoid-mediated", "induction", "of", "interleukin-1", "receptor", "synthesis", "." ]
[ 0, 0, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0 ]
16457
[ "Transcription", "specific", "differences", "visualized", "by", "fluorescence", "in", "situ", "hybridization", "pattern", "on", "interphase", "nuclei", "of", "different", "cell", "types", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16458
[ "Application", "of", "a", "``", "formamide", "free", "''", "and", "thus", "``", "material", "preserving", "''", "in", "situ", "hybridization", "technique", "using", "the", "cDNA", "of", "the", "myf3", "gene", "revealed", "the", "following", "results", ":", "Human", "rhabdomyosarcoma", "cells", ",", "characterized", "by", "a", "high", "expression", "of", "myf3", "show", "intensive", "hybridization", "signals", "in", "their", "interphase", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 7, 8, 0, 0, 0, 0, 0, 13, 14, 14, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0 ]
16459
[ "RNase", "treatment", "prior", "to", "hybridization", "considerably", "reduces", "the", "size", "of", "this", "signals", "." ]
[ 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16460
[ "In", "comparison", ",", "isolated", "nuclei", "of", "human", "lymphocytes", "in", "which", "no", "need", "for", "the", "expression", "of", "this", "gene", "exists", ",", "show", "barely", "hybridization", "signals", "." ]
[ 0, 0, 0, 0, 0, 0, 13, 14, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16461
[ "Correspondingly", ",", "RNase", "treatment", "had", "no", "effect", "on", "hybridization", "pattern", "at", "all", "." ]
[ 0, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16462
[ "In", "conclusion", "an", "increased", "transcription", "efficiency", "of", "a", "cell", "type", "specific", "gene", "is", "accompanied", "by", "a", "higher", "hybridization", "accessibility", "in", "the", "corresponding", "cell", "nuclei", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16463
[ "Oncogenicity", "of", "human", "papillomavirus-", "or", "adenovirus-transformed", "cells", "correlates", "with", "resistance", "to", "lysis", "by", "natural", "killer", "cells", "." ]
[ 0, 0, 11, 12, 12, 12, 12, 0, 0, 0, 0, 0, 0, 13, 14, 14, 0 ]
16464
[ "The", "reasons", "for", "the", "dissimilar", "oncogenicities", "of", "human", "adenoviruses", "and", "human", "papillomaviruses", "(", "HPV", ")", "in", "humans", "are", "unknown", "but", "may", "relate", "to", "differences", "in", "the", "capacities", "of", "the", "E1A", "and", "E7", "proteins", "to", "target", "cells", "for", "rejection", "by", "the", "host", "natural", "killer", "(", "NK", ")", "cell", "response", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 0, 15, 16, 0, 13, 14, 0, 0, 0, 0, 13, 14, 14, 14, 14, 14, 14, 0, 0 ]
16465
[ "As", "one", "test", "of", "this", "hypothesis", ",", "we", "compared", "the", "abilities", "of", "E1A", "-and", "E7", "-expressing", "human", "fibroblastic", "or", "keratinocyte-derived", "human", "cells", "to", "be", "selectively", "killed", "by", "either", "unstimulated", "or", "interferon", "(", "IFN", ")", "-activated", "NK", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 0, 15, 0, 11, 12, 12, 12, 12, 12, 0, 0, 0, 0, 0, 0, 13, 0, 11, 12, 12, 12, 12, 12, 12, 0 ]
16466
[ "Cells", "expressing", "the", "E1A", "oncoprotein", "were", "selectively", "killed", "by", "unstimulated", "NK", "cells", ",", "while", "the", "same", "parental", "cells", "but", "expressing", "the", "HPV", "type", "16", "(", "HPV-16", ")", "or", "HPV-18", "E7", "oncoprotein", "were", "resistant", "to", "NK", "cell", "lysis", "." ]
[ 0, 0, 0, 15, 16, 0, 0, 0, 0, 11, 12, 12, 0, 0, 0, 0, 13, 14, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0 ]
16467
[ "The", "ability", "of", "IFN-activated", "NK", "cells", "to", "selectively", "kill", "virally", "transformed", "cells", "depends", "on", "IFN", "'s", "ability", "to", "induce", "resistance", "to", "NK", "cell", "lysis", "in", "normal", "(", "i.e.", ",", "non-viral", "oncogene-expressing", ")", "but", "not", "virally", "transformed", "cells", "." ]
[ 0, 0, 0, 11, 12, 12, 0, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 11, 12, 0, 0, 0, 11, 12, 12, 0 ]
16468
[ "E1A", "blocked", "IFN", "'s", "induction", "of", "cytolytic", "resistance", ",", "resulting", "in", "the", "selective", "lysis", "of", "adenovirus-transformed", "cells", "by", "IFN-activated", "NK", "cells", "." ]
[ 15, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 11, 12, 0, 11, 12, 12, 0 ]
16469
[ "The", "extent", "of", "IFN", "-induced", "NK", "cell", "killing", "of", "E1A-expressing", "cells", "was", "proportional", "to", "the", "level", "of", "E1A", "expression", "and", "correlated", "with", "the", "ability", "of", "E1A", "to", "block", "IFN", "-stimulated", "gene", "expression", "in", "target", "cells", "." ]
[ 0, 0, 0, 15, 0, 0, 0, 0, 0, 11, 12, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 15, 0, 0, 0, 0, 13, 14, 0 ]
16470
[ "In", "contrast", ",", "E7", "blocked", "neither", "IFN", "-stimulated", "gene", "expression", "nor", "IFN", "'s", "induction", "of", "cytolytic", "resistance", ",", "thereby", "precluding", "the", "selective", "lysis", "of", "HPV-transformed", "cells", "by", "IFN-activated", "NK", "cells", "." ]
[ 0, 0, 0, 15, 0, 0, 15, 0, 0, 0, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 11, 12, 0, 11, 12, 12, 0 ]
16471
[ "In", "conclusion", ",", "E1A", "expression", "marks", "cells", "for", "destruction", "by", "the", "host", "NK", "cell", "response", ",", "whereas", "the", "E7", "oncoprotein", "lacks", "this", "activity", "." ]
[ 0, 0, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 13, 14, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0 ]
16472
[ "Regulation", "of", "IkB", "alpha", "phosphorylation", "by", "PKC-", "and", "Ca", "(", "2+", ")", "-dependent", "signal", "transduction", "pathways", "." ]
[ 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16473
[ "The", "Ca", "(", "2+", ")", "-dependent", "phosphatase", "calcineurin", ",", "a", "target", "of", "FK506", "and", "CsA", ",", "synergizes", "with", "PKC", "-induced", "activation", "of", "nuclear", "factor", "(", "NF", ")", "-kappa", "B", "in", "T", "cell", "lines", "." ]
[ 0, 15, 16, 16, 16, 16, 16, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 15, 16, 16, 16, 16, 16, 16, 0, 11, 12, 12, 0 ]
16474
[ "We", "have", "investigated", "whether", "this", "synergy", "is", "present", "in", "other", "cell", "types", "and", "the", "mechanism", "(", "s", ")", "by", "which", "these", "two", "pathways", "lead", "to", "NF-kappa", "B", "activation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0 ]
16475
[ "While", "this", "synergy", "is", "present", "in", "other", "cell", "types", ",", "in", "the", "monocytic", "cell", "line", "U937", "calcineurin", "is", "also", "sufficient", "to", "activate", "NF-kappa", "B", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 11, 12, 12, 12, 15, 0, 0, 0, 0, 0, 15, 16, 0 ]
16476
[ "Having", "previously", "shown", "that", "Ca", "(", "2+", ")", "-", "and", "PKC-dependent", "pathways", "synergize", "by", "accelerating", "the", "degradation", "of", "IkB", "alpha", ",", "we", "focused", "on", "the", "regulation", "of", "IkB", "alpha", "phosphorylation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0 ]
16477
[ "While", "PKC", "-dependent", "pathways", "sequentially", "result", "in", "the", "phosphorylation", "and", "in", "an", "incomplete", "degradation", "of", "IkB", "alpha", "in", "T", "cell", "lines", ",", "co-activation", "of", "Ca", "(", "2+", ")", "-dependent", "pathways", "accelerates", "the", "rate", "of", "IkB", "alpha", "phosphorylation", "and", "results", "in", "its", "complete", "degradation", "." ]
[ 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 11, 12, 12, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0, 0 ]
16478
[ "Activation", "of", "Ca", "(", "2+", ")", "-dependent", "pathways", "alone", "do", "not", "result", "in", "the", "phosphorylation", "and/or", "degradation", "of", "IkB", "alpha", "in", "Jurkat", "T", "or", "in", "U937", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 11, 12, 0, 0, 11, 12, 0 ]
16479
[ "Treatment", "of", "T", "cells", "with", "the", "selective", "PKC", "inhibitor", "GF109203X", "abrogates", "the", "PMA-induced", "IkB", "alpha", "phosphorylation/degradation", "irrespective", "of", "activation", "of", "Ca", "(", "2+", ")", "-dependent", "pathways", ",", "but", "not", "the", "phosphorylation", "and", "degradation", "of", "IkB", "alpha", "induced", "by", "TNF-alpha", ",", "a", "PKC", "-independent", "stimulus", "." ]
[ 0, 0, 13, 14, 0, 0, 0, 15, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 15, 0, 0, 15, 0, 0, 0 ]
16480
[ "Contrary", "to", "the", "interaction", "with", "PKC", ",", "Ca", "(", "2+", ")", "-dependent", "pathways", "synergize", "with", "TNF-alpha", "not", "at", "the", "level", "of", "IkB", "alpha", "phosphorylation", ",", "but", "at", "the", "level", "of", "its", "degradation", "." ]
[ 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16481
[ "These", "results", "indicate", "that", "Ca", "(", "2+", ")", "-dependent", "pathways", ",", "including", "the", "phosphatase", "calcineurin", ",", "participate", "in", "the", "regulation", "of", "NF-kappa", "B", "in", "a", "cell", "specific", "fashion", "and", "synergize", "with", "PKC", "-dependent", "and", "-independent", "pathways", "at", "the", "level", "of", "IkB", "alpha", "phosphorylation", "and", "degradation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 15, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0 ]
16482
[ "Vitamin", "E", "therapy", "of", "acute", "CCl4-induced", "hepatic", "injury", "in", "mice", "is", "associated", "with", "inhibition", "of", "nuclear", "factor", "kappa", "B", "binding", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 16, 16, 0, 0 ]
16483
[ "Oxidative", "stress", ",", "with", "reactive", "oxygen", "intermediate", "formation", ",", "may", "represent", "a", "common", "mechanism", "by", "which", "liver", "injury", "is", "induced", "by", "diverse", "etiologies", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16484
[ "Oxidative", "stress", "enhances", "nuclear", "factor", "kappa", "B", "(", "NF-kappa", "B", ")", "activity", ",", "and", "NF-kappa", "B", "activity", "has", "been", "shown", "to", "enhance", "the", "expression", "of", "cytotoxic", "cytokines", "." ]
[ 0, 0, 0, 15, 16, 16, 16, 0, 15, 16, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0 ]
16485
[ "Acute", "hepatic", "injury", "caused", "by", "reactive", "oxygen", "intermediate", "production", "was", "induced", "by", "an", "intraperitoneal", "injection", "of", "CCl4", "in", "mice", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16486
[ "This", "injury", "was", "significantly", "inhibited", "by", "intravenous", "pretreatment", "of", "the", "mice", "with", "a", "water-soluble", "emulsion", "of", "alpha-tocopherol", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16487
[ "Alpha-tocopherol", "treatment", "of", "the", "mice", "given", "the", "CCl4", "also", "reduced", "the", "NF-kappa", "B", "binding", "to", "levels", "approaching", "those", "found", "in", "normal", "mice", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16488
[ "In", "vitro", "treatment", "of", "a", "monocyte/macrophage", "cell", "line", "with", "CCl4", "led", "to", "enhanced", "NF-kappa", "B", "binding", "and", "an", "increase", "in", "tumor", "necrosis", "factor-alpha", "(", "TNF-alpha", ")", "messenger", "RNA", "levels", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 0 ]
16489
[ "Liver", "specimens", "taken", "from", "patients", "with", "acute", "fulminant", "hepatitis", "had", "markedly", "increased", "NF-kappa", "B", "binding", "activity", "in", "comparison", "with", "the", "binding", "of", "normal", "livers", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16490
[ "These", "data", "demonstrate", "that", "abolishing", "acute", "hepatic", "injury", "with", "alpha-tocopherol", ",", "a", "free", "radical", "scavenger", ",", "also", "eliminated", "increased", "NF-kappa", "B", "binding", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0 ]
16491
[ "It", "is", "tempting", "to", "speculate", "that", "enhanced", "NF-kappa", "B", "expression", "caused", "by", "free", "radical", "production/oxidative", "stress", "may", "modulate", "liver", "injury", ",", "perhaps", "through", "an", "effect", "on", "cytotoxic", "cytokine", "synthesis", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0 ]
16492
[ "Immunosuppression", "by", "glucocorticoids", ":", "inhibition", "of", "NF-kappa", "B", "activity", "through", "induction", "of", "I", "kappa", "B", "synthesis", "[", "see", "comments", "]" ]
[ 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 0, 15, 16, 16, 0, 0, 0, 0, 0 ]
16493
[ "Glucocorticoids", "are", "among", "the", "most", "potent", "anti-inflammatory", "and", "immunosuppressive", "agents", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16494
[ "They", "inhibit", "synthesis", "of", "almost", "all", "known", "cytokines", "and", "of", "several", "cell", "surface", "molecules", "required", "for", "immune", "function", ",", "but", "the", "mechanism", "underlying", "this", "activity", "has", "been", "unclear.", "Here", "it", "is", "shown", "that", "glucocorticoids", "are", "potent", "inhibitors", "of", "nuclear", "factor", "kappa", "B", "(", "NF-kappa", "B", ")", "activation", "in", "mice", "and", "cultured", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 15, 16, 16, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 16, 16, 0, 15, 16, 0, 0, 0, 0, 0, 11, 12, 0 ]
16495
[ "This", "inhibition", "is", "mediated", "by", "induction", "of", "the", "I", "kappa", "B", "alpha", "inhibitory", "protein", ",", "which", "traps", "activated", "NF-kappa", "B", "in", "inactive", "cytoplasmic", "complexes", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 15, 16, 16, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 15, 16, 16, 0 ]
16496
[ "Because", "NF-kappa", "B", "activates", "many", "immunoregulatory", "genes", "in", "response", "to", "pro-inflammatory", "stimuli", ",", "the", "inhibition", "of", "its", "activity", "can", "be", "a", "major", "component", "of", "the", "anti-inflammatory", "activity", "of", "glucocorticoids", "." ]
[ 0, 15, 16, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16497
[ "Transcriptional", "repression", "of", "the", "interleukin-2", "gene", "by", "vitamin", "D3", ":", "direct", "inhibition", "of", "NFATp/AP-1", "complex", "formation", "by", "a", "nuclear", "hormone", "receptor", "." ]
[ 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 15, 16, 0, 0, 0, 15, 16, 16, 0 ]
16498
[ "T-lymphocyte", "proliferation", "is", "suppressed", "by", "1", ",", "25-dihydroxyvitamin", "D3", "[", "1", ",", "25", "(", "OH", ")", "2D3", "]", ",", "the", "active", "metabolite", "of", "vitamin", "D3", ",", "and", "is", "associated", "with", "a", "decrease", "in", "interleukin", "2", "(", "IL-2", ")", ",", "gamma", "interferon", ",", "and", "granulocyte-macrophage", "colony-stimulating", "factor", "mRNA", "levels", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
16499
[ "We", "report", "here", "that", "1", ",", "25", "(", "OH", ")", "2D3-mediated", "repression", "in", "Jurkat", "cells", "is", "cycloheximide", "resistant", ",", "suggesting", "that", "it", "is", "a", "direct", "transcriptional", "repressive", "effect", "on", "IL-2", "expression", "by", "the", "vitamin", "D3", "receptor", "(", "VDR", ")", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 11, 12, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 15, 0, 0, 0, 15, 16, 16, 0, 15, 0, 0 ]