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Acute and Chronic Macrophage Differentiation Modulates TREM2 in a Personalized Alzheimer's Patient-Derived Assay.

Neuroinflammation plays a pivotal role in the pathogenesis of Alzheimer`s disease (AD). Brain macrophage populations differentially modulate the immune response to AD pathology according to the disease stage. Triggering receptor expressed on myeloid cells 2 (TREM2) is known to play a protective role in AD and has been postulated as a putative therapeutic target. Whether, and to which extent TREM2 expression can be modulated in the aged macrophage population of the brain is unknown, emphasizing the need for a human, patient-specific model. Using cells from AD patients and matched controls (CO) we designed an assay based on monocyte-derived macrophages to mimic brain-infiltrating macrophages and to assess the individualized TREM2 synthesis in vitro. We systematically assessed the effects of short-term (acute-2 days) and long-term (chronic-10 days) M1- (LPS), M2- (IL-10, IL-4, TGF-β), and M0- (vehicle) macrophage differentiation on TREM2 synthesis. Moreover, the effects of retinoic acid (RA), a putative TREM2 modulator, on individualized TREM2 synthesis were assessed. We report increased TREM2 synthesis after acute M2- compared to M1-differentiation in CO- but not AD-derived cells. Chronic M2- and M0-differentiation however resulted in an increase of TREM2 synthesis in both AD- and CO-derived cells while chronic M1-differentiation increased TREM2 in AD-derived cells only. Moreover, chronic M2- and M0-differentiation improved the amyloid-β (Aβ) uptake of the CO-derived whereas M1-differentiation of the AD-derived cells. Interestingly, RA-treatment did not modulate TREM2. In the age of personalized medicine, our individualized model could be used to screen for potential drug-mediated treatment responses in vitro. Triggering receptor expressed on myeloid cells 2 (TREM2) has been postulated as a putative therapeutic target in Alzheimer's disease (AD). Using cells from AD patients and matched controls (CO), we designed a monocyte-derived macrophages (Mo-MФs) assay to assess the individualized TREM2 synthesis in vitro. We report increased TREM2 synthesis after acute M2- compared to M1- macrophage differentiation in CO- but not AD-derived cells. Chronic M2- and M0- differentiation however resulted in an increase of TREM2 synthesis in both AD- and CO-derived cells while chronic M1-differentiation increased TREM2 in AD-cells only.

Decision-making under ambiguity and risk and executive functions in Parkinson's disease patients: A scoping review of the studies investigating the Iowa Gambling Task and the Game of Dice.

Evidence shows that patients affected by Parkinson's disease (PD) display the tendency toward making risky choices. This is due, at least in part, to the pathophysiological characteristics of the disease that affects neural areas underlying decision making (DM), in which a pivotal role is played by nonmotor corticostriatal circuits and dopamine. Executive functions (EFs), which can be impaired by PD as well, may sustain optimal choices in DM processes. However, few studies have investigated whether EFs can support PD patients to make good decisions. Adopting the scoping review approach, the present article is designed to deepen the cognitive mechanisms of DM under conditions of ambiguity and risk (that are conditions common to everyday life decisions) in PD patients without impulse control disorders. We focused our attention on the Iowa Gambling Task and the Game of Dice Task, because they are the most commonly used and reliable tasks to assess DM under ambiguity and under risk, respectively, and analyzed the performances in such tasks and their relationships with EFs tests in PD patients. The analysis supported the relationships between EFs and DM performance, especially when a higher cognitive load is required to make optimal decisions, as it happens under conditions of risk. Possible knowledge gaps and further research directions are suggested to better understand DM mechanisms in PD sustaining patients' cognitive functioning and preventing negative consequences in everyday life derived from suboptimal decisions.

Hierarchical status is rapidly assessed from behaviourally dominant faces.

Recognition of social hierarchy is a key feature that helps us navigate through our complex social environment. Neuroimaging studies have identified brain structures involved in the processing of hierarchical stimuli, but the precise temporal dynamics of brain activity associated with such processing remains largely unknown. In this investigation, we used event-related potentials (ERPs) to examine the effect of social hierarchy on the neural responses elicited by dominant and nondominant faces. Participants played a game where they were led to believe that they were middle-rank players, responding alongside other alleged players, whom they perceived as higher or lower-ranking. ERPs were examined in response to dominant and nondominant faces, and low-resolution electromagnetic tomography (LORETA) was used to identify the implicated brain areas. The results revealed that the amplitude of the N170 component was enhanced for faces of dominant individuals, showing that hierarchy influences the early stages of face processing. A later component, the late positive potential (LPP) appearing between 350-700 ms, also was enhanced for faces of higher-ranking players. Source localisation suggested that the early modulation was due to an enhanced response in limbic regions. These findings provide electrophysiological evidence for enhanced early visual processing of socially dominant faces.

Functional and structural responses of plankton communities toward consecutive experimental heatwaves in Mediterranean coastal waters.

The frequency of marine heatwaves (HWs) is projected to increase in the Mediterranean Sea over the next decades. An in situ mesocosm experiment was performed in a Mediterranean lagoon for 33 days. Three mesocosms were used as controls following the natural temperature of the lagoon. In three others, two HWs of + 5 °C compared to the controls were applied from experimental day (d) 1 to d5 (HW1) and from d11 to d15 (HW2). High-frequency data of oxygen, chlorophyll-a (chl-a), temperature, salinity and light from sensors immersed in all mesocosms were used to calculate gross primary production (GPP), respiration (R) and phytoplankton growth (µ) and loss (L) rates. Nutrients and phytoplankton community structure from pigments were also analyzed. HW1 significantly increased GPP, R, chl-a, µ and L by 7 to 38%. HW2 shifted the system toward heterotrophy by only enhancing R. Thus, the effects of the first HW resulted in the attenuation of those of a second HW on phytoplankton processes, but not on community respiration, which was strongly regulated by temperature. In addition, natural phytoplankton succession from diatoms to haptophytes was altered by both HWs as cyanobacteria and chlorophytes were favored at the expense of haptophytes. These results indicate that HWs have pronounced effects on Mediterranean plankton communities.

General anesthesia with propofol during oocyte retrieval and in vitro fertilization outcomes: retrospective cohort study.

General anesthesia is frequently administered during oocyte retrieval. Its effects on the outcomes of IVF cycles are uncertain. This study investigated whether administration of general anesthesia (specifically propofol) during oocyte retrieval affects IVF outcomes. A total of 245 women undergoing IVF cycles were included in this retrospective cohort study. IVF outcomes of 129 women who underwent oocyte retrieval under propofol anesthesia and 116 without anesthesia were compared. Data were adjusted for age, BMI, estradiol on triggering day and total gonadotropin dose. The primary outcomes were fertilization, pregnancy and live birth rates. A secondary outcome was the efficiency of follicle retrieval associated with the use of anesthesia. Fertilization rate was lower in retrievals under anesthesia compared to without (53.4% ± 34.8 vs. 63.7% ± 33.6, respectively; p = 0.02). There was no significant difference in the ratio of expected to retrieved oocytes between retrievals with and without anesthesia (0.8 ± 0.4 vs. 0.8 ± 0.8, respectively, p = 0.96). The differences in pregnancy and live birth rates between the groups were not statistically significant. General anesthesia administered during oocyte retrieval may have adverse effects on the fertilization potential of oocytes. This impact on the developmental potential of oocytes may lead to negative IVF outcomes and should be investigated further.

Oncological outcomes of laparoscopic versus open radical total gastrectomy for upper-middle gastric cancer after neoadjuvant chemotherapy: a study of real-world data.

Laparoscopic technique has been increasingly used in gastrectomy, but the safety and feasibility of the laparoscopic total gastrectomy (LTG) for advanced proximal gastric cancer (PGC) after neoadjuvant chemotherapy (NAC) is unclear.

Esophageal dilation with EsoFLIP is faster than CRE balloon dilation combined with EndoFLIP in children.

Controlled radial expansion (CRE) balloon dilators are traditionally used to dilate esophageal strictures during an esophagogastroduodenoscopy (EGD). EndoFLIP is a diagnostic tool used during an EGD to measure important parameters of the gastrointestinal lumen, capable of assessing treatment before and after dilation. EsoFLIP is a related device that combines a balloon dilator with high-resolution impedance planimetry to provide some of the luminal parameters in real time during dilation. We sought to compare procedure time, fluoroscopy time, and safety profile of esophageal dilation using either CRE balloon dilation combined with EndoFLIP (E + CRE) versus EsoFLIP alone.

Comprehensive analysis of ferroptosis-related genes for clinical and biological significance in hepatocellular carcinoma.

This study aims to build a prognostic model of hepatocellular carcinoma (HCC) with ferroptosis-associated genes and explore their molecular function.

Application of AtMYB75 as a reporter gene in the study of symbiosis between tomato and Funneliformis mosseae.

Composite plants containing transgenic hairy roots produced with Agrobacterium rhizogenes-mediated transformation have become an important method to study the interaction between plants and arbuscular mycorrhizal fungi (AMF). Not all hairy roots induced by A. rhizogenes are transgenic, however, which leads to requirement of a binary vector to carry a reporter gene to distinguish transgenic roots from non-transformed hairy roots. The beta-glucuronidase gene (GUS) and fluorescent protein gene often are used as reporter markers in the process of hairy root transformation, but they require expensive chemical reagents or imaging equipment. Alternatively, AtMYB75, an R2R3 MYB transcription factor from Arabidopsis thaliana, recently has been used as a reporter gene in hairy root transformation in some leguminous plants and can cause anthocyanin accumulation in transgenic hairy roots. Whether AtMYB75 can be used as a reporter gene in the hairy roots of tomato and if the anthocyanins accumulating in the roots will affect AMF colonization, however, are still unknown. In this study, the one-step cutting method was used for tomato hairy root transformation by A.rhizogenes. It is faster and has a higher transformation efficiency than the conventional method. AtMYB75 was used as a reporter gene in tomato hairy root transformation. The results showed that the overexpression of AtMYB75 caused anthocyanin accumulation in the transformed hairy roots. Anthocyanin accumulation in the transgenic hairy roots did not affect their colonization by the arbuscular mycorrhizal fungus, Funneliformis mosseae strain BGC NM04A, and there was no difference in the expression of the AMF colonization marker gene SlPT4 in AtMYB75 transgenic roots and wild-type roots. Hence, AtMYB75 can be used as a reporter gene in tomato hairy root transformation and in the study of symbiosis between tomato and AMF.

Diagnostic and prognostic relevance of using large gene panels in the genetic testing of patients with dilated cardiomyopathy.

It was previously suggested that increasing the number of genes on diagnostic gene panels could increase the genetic yield in patient with dilated cardiomyopathy (DCM). We explored the diagnostic and prognostic relevance of testing DCM patients with an expanded gene panel. The current study included 225 consecutive DCM patients who had no genetic diagnosis after a 48-gene cardiomyopathy-panel. These were then evaluated using an expanded gene panel of 299 cardiac-associated genes. A likely pathogenic/pathogenic (P/LP) variant was detected in 13 patients. Five variants were reclassifications of variants found in genes which were already detected using the 48 gene panel. Only one of the other eight variants could explain the phenotype of the patient (KCNJ2). The panel detected 186 VUSs in 127 patients (of which 6 also had a P/LP variant). The presence of a VUS was significantly associated with the combined end-point of mortality, heart failure hospitalization, heart transplantation or life-threatening arrhythmias(HR, 2.04 [95% CI, 1.15 to 3.65]; p = 0.02). The association of a VUS with prognosis remained when we only included VUSs in robust DCM-associated genes (high suspicious VUSs), but disappeared when we only included VUSs in non-robust DCM-associated genes (low suspicious VUSs), highlighting the importance of weighing of VUSs. Overall, the use of large gene panels for genetic testing in DCM does not increase the diagnostic yield, although a VUS in a robust DCM-associated gene is associated with an adverse prognosis. Altogether, current diagnostic gene panels should be limited to the robust DCM-associated genes.

Association of endometriosis with genital human papillomavirus infection in US women: a national population-based study.

The prevalence of genital human papillomavirus (HPV) in women with endometriosis has never been reported in a national representative survey. We aimed to investigate the association of endometriosis with the prevalence of HPV. We analyzed the data on 1768 women (representing 43,824,157 women) in the United States aged 20-54 years from the National Health and Nutrition Examination Survey in the prevaccination era (2003-2006). The diagnosis of endometriosis was based on a self-report. The prevalence of any HPV in women with endometriosis did not differ from that in women without endometriosis after controlling for potential confounders such as age, ethnicity, family income, marital status, and the number of deliveries (adjusted prevalence ratio (aPR) 0.84, 95% confidence interval (CI) 0.61-1.15). No significant association was found between the prevalence of high-risk HPV and the diagnosis of endometriosis (aPR 0.71, 95% CI 0.44-1.14). If the participants were not covered by health insurance, the prevalence of any HPV infection in women with endometriosis was higher than in those without endometriosis (aPR 1.44, 95% CI 0.94-2.20). In contrast, in a subgroup who had health insurance, a lower prevalence of any HPV infection was observed in women with endometriosis (aPR 0.71, 95% CI 0.50-1.03), and P for interaction was statistically significant (P = 0.01). There was no association between endometriosis and HPV infection in this study of HPV vaccine-naïve women of reproductive age. The association was not different by the type of HPV. However, access to healthcare may modify the association between endometriosis and HPV infection.

Single-cell and spatial transcriptomics: deciphering brain complexity in health and disease.

In the past decade, single-cell technologies have proliferated and improved from their technically challenging beginnings to become common laboratory methods capable of determining the expression of thousands of genes in thousands of cells simultaneously. The field has progressed by taking the CNS as a primary research subject - the cellular complexity and multiplicity of neuronal cell types provide fertile ground for the increasing power of single-cell methods. Current single-cell RNA sequencing methods can quantify gene expression with sufficient accuracy to finely resolve even subtle differences between cell types and states, thus providing a great tool for studying the molecular and cellular repertoire of the CNS and its disorders. However, single-cell RNA sequencing requires the dissociation of tissue samples, which means that the interrelationships between cells are lost. Spatial transcriptomic methods bypass tissue dissociation and retain this spatial information, thereby allowing gene expression to be assessed across thousands of cells within the context of tissue structural organization. Here, we discuss how single-cell and spatially resolved transcriptomics have been contributing to unravelling the pathomechanisms underlying brain disorders. We focus on three areas where we feel these new technologies have provided particularly useful insights: selective neuronal vulnerability, neuroimmune dysfunction and cell-type-specific treatment response. We also discuss the limitations and future directions of single-cell and spatial RNA sequencing technologies.

Unraveling the neural basis of spatial orientation in arthropods.

The neural basis underlying spatial orientation in arthropods, in particular insects, has received considerable interest in recent years. This special issue of the Journal of Comparative Physiology A seeks to take account of these developments by presenting a collection of eight review articles and eight original research articles highlighting hotspots of research on spatial orientation in arthropods ranging from flies to spiders and the underlying neural circuits. The contributions impressively illustrate the wide range of tools available to arthropods extending from specific sensory channels to highly sophisticated neural computations for mastering complex navigational challenges.

Multi-modality treatment approach for paediatric AVMs with quality-of-life outcome measures.

Despite the potentially devastating and permanently disabling effects of paediatric arteriovenous malformations (pAVMs), there is a paucity of studies reporting long-term quality-of-life (QoL) outcomes in AVM patients. We aim to evaluate the management strategies for paediatric intracranial pAVMs in the UK and long-term QoL outcomes using a validated paediatric quality-of-life outcome measure.

Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions.

Glucose is vital for life, serving as both a source of energy and carbon building block for growth. When glucose is limiting, alternative nutrients must be harnessed. To identify mechanisms by which cells can tolerate complete loss of glucose, we performed nutrient-sensitized genome-wide genetic screens and a PRISM growth assay across 482 cancer cell lines. We report that catabolism of uridine from the medium enables the growth of cells in the complete absence of glucose. While previous studies have shown that uridine can be salvaged to support pyrimidine synthesis in the setting of mitochondrial oxidative phosphorylation deficiency

A temperate Earth-sized planet with tidal heating transiting an M6 star.

Temperate Earth-sized exoplanets around late-M dwarfs offer a rare opportunity to explore under which conditions planets can develop hospitable climate conditions. The small stellar radius amplifies the atmospheric transit signature, making even compact secondary atmospheres dominated by N

Lysine catabolism reprograms tumour immunity through histone crotonylation.

Cancer cells rewire metabolism to favour the generation of specialized metabolites that support tumour growth and reshape the tumour microenvironment

Observation of hydrodynamization and local prethermalization in 1D Bose gases.

Hydrodynamics accurately describe relativistic heavy-ion collision experiments well before local thermal equilibrium is established

Orbital inflammation following COVID-19 vaccination: A case series and literature review.

The purpose of the study was to report three cases of orbital inflammation following administration of the COVID-19 vaccination, manifesting as Tolosa-Hunt syndrome (THS) and orbital myositis.

Cardiac contractility modulation: an effective treatment strategy for heart failure beyond reduced left ventricular ejection fraction?

Heart failure (HF) with preserved ejection fraction (HFpEF) causes a progressive limitation of functional capacity, poor quality of life (QoL) and increased mortality, yet unlike HF with reduced ejection fraction (HFrEF) there are no effective device-based therapies. Both HFrEF and HFpEF are associated with dysregulations in myocardial cellular calcium homeostasis and modifications in calcium-handling proteins, leading to abnormal myocardial contractility and pathological remodelling. Cardiac contractility modulation (CCM) therapy, based on a pacemaker-like implanted device, applies extracellular electrical stimulation to myocytes during the absolute refractory period of the action potential, which leads to an increase in cytosolic peak calcium concentrations and thereby the force of isometric contraction promoting positive inotropism. Subgroup analysis of CCM trials in HFrEF has demonstrated particular benefits in patients with LVEF between 35% and 45%, suggesting its potential effectiveness also in patients with higher LVEF values. Available evidence on CCM in HFpEF is still preliminary, but improvements in terms of symptoms and QoL have been observed. Future large, dedicated, prospective studies are needed to evaluate the safety and efficacy of this therapy in patients with HFpEF.

Headache Associated with PPE During COVID-19 Pandemic in Health Care Workers.

In the pandemic coronavirus disease 2019 (COVID-19), health care workers (HCWs) are at very high risk. Personal protective equipment (PPE) and masks are not only difficult to wear while working but also causes various complications. The present self-administered questionnaire- based study aimed to explore the headache and complications in HCWs on wearing PPE during the COVID-19 pandemic.

Myositis developing after Covid-19 mRNA vaccine: Case Report.

Vaccine-related side effects are common. Usually, pain, edema, redness and tenderness may be seen at the injection site. Symptoms such as fever, fatigue, myalgia may occur. The coronavirus 2019 disease (Covid-19) has affected many people around the world. Although the vaccines that have been used play an active role in the fight against the pandemic, adverse events still continue to be reported. We present a 21-year-old patient who was diagnosed as having myositis after receiving covid vaccine with complaints of pain in her left arm two days after the 2nd dose of BNT162b2 mRNA Covid-19 vaccine, followed by inability to stand up from sitting and squatting and difficulty in going up and down stairs. Keywords: vaccine, myositis, creatine kinase, IVIG.

HPV-infection status and urinary incontinence: a population-based analysis of the NHANES 2005-2016.

Urinary incontinence is a common condition and reduces the quality of life. The purpose of this study was to assess the association between HPV infection and urinary incontinence among adult women in the USA.

Efficacy and Safety of Brolucizumab, Aflibercept, and Ranibizumab for the Treatment of Patients with Visual Impairment Due to Diabetic Macular Oedema: A Systematic Review and Network Meta-Analysis.

Key clinical guidelines recommend anti-vascular endothelial growth factor (VEGF) therapy as first-line treatment for visual impairment due to diabetic macular oedema (DMO). A systematic literature review (SLR) and network meta-analysis (NMA) were conducted comparing the relative efficacy of the anti-VEGF brolucizumab with a focused network of the most relevant comparator dosing regimens approved in countries other than the USA (aflibercept, ranibizumab). The safety and tolerability of brolucizumab were also assessed.

Effects of different subcutaneous sites on heterotopic autotransplantation of canine ovarian tissue.

Ovarian tissue transplantation makes it possible to restore fertility; however, the success of this technique depends on the transplant region used. Therefore, this study aimed to evaluate the effect of two subcutaneous regions on canine ovarian transplantation, pinna (Pi) and neck (Ne), for 7 and 15 days. Ovaries collected by ovariosalpingohysterectomy were fragmented using a punch device. Fresh fragments were fixed, and the others were immediately grafted onto the animal itself in the Pi and Ne regions for 7 and 15 days. Recovered fragments were evaluated for histology (morphology, development and stromal density), picrosirius (collagen fibers), and immunohistochemistry (fibrosis and cell proliferation). The results showed that follicular normality rates were lower in Pi-7 (78%) vs. control (90%) and Pi-15 (86%), similar in Ne-7 (92%) and superior in Ne-15 (97%) compared to the control, with the effect of the region Ne (94%) superior (P < 0.05) to Pi (82%). Stromal density reduced in both regions vs. control but was similar within 15 days. Fragments from both regions showed higher fibronectin labeling and deposition of type I and lower type III collagen fibers (P < 0.05) vs. control. Proliferation rates in Ne-7 were higher (P < 0.05) than in control, and Pi-15 was higher (P < 0.05) than Ne-15. In conclusion, the pinna may be a region with greater potential than the neck after a 15-day autotransplantation of canine ovarian tissue.

The C-terminal 32-mer fragment of hemoglobin alpha is an amyloidogenic peptide with antimicrobial properties.

Antimicrobial peptides (AMPs) are major components of the innate immune defense. Accumulating evidence suggests that the antibacterial activity of many AMPs is dependent on the formation of amyloid-like fibrils. To identify novel fibril forming AMPs, we generated a spleen-derived peptide library and screened it for the presence of amyloidogenic peptides. This approach led to the identification of a C-terminal 32-mer fragment of alpha-hemoglobin, termed HBA(111-142). The non-fibrillar peptide has membranolytic activity against various bacterial species, while the HBA(111-142) fibrils aggregated bacteria to promote their phagocytotic clearance. Further, HBA(111-142) fibrils selectively inhibited measles and herpes viruses (HSV-1, HSV-2, HCMV), but not SARS-CoV-2, ZIKV and IAV. HBA(111-142) is released from its precursor by ubiquitous aspartic proteases under acidic conditions characteristic at sites of infection and inflammation. Thus, HBA(111-142) is an amyloidogenic AMP that may specifically be generated from a highly abundant precursor during bacterial or viral infection and may play an important role in innate antimicrobial immune responses.

Is there a relation between stillbirth and low levels of vitamin D in the population? A bi-national follow-up study of vitamin D fortification.

Stillbirth has been associated with low plasma vitamin D. Both Sweden and Finland have a high proportion of low plasma vitamin D levels (< 50 nmol/L). We aimed to assess the odds of stillbirth in relation to changes in national vitamin D fortification.

Modifications in steroid and triterpenoid metabolism in Calendula officinalis plants and hairy root culture in response to chitosan treatment.

Chitosan, a deacetylated derivative of chitin, is one of the most preferred biopolymers for use as biostimulants and biofertilizers in organic agriculture and as elicitors to enhance the productivity of plant in vitro cultures. Valued as a non-toxic, biodegradable, and environment-friendly agent, it is widely applied to improve plant growth and yield, the content of bioactive specialized metabolites, and resistance to stress conditions and pathogens. However, the influence of chitosan on the growth-defense trade-off, particularly the interplay between steroid and triterpenoid metabolism, has not been extensively investigated.

Profiling the impact of the promoters on CRISPR-Cas12a system in human cells.

The plasmid vector platform is the most commonly used vector for the expression of the versatile CRISPR-Cas technique and the promoter is a crucial element for the expression vector, thus profiling the impact of the promoters on CRISPR editors provides the basic information for the gene-editing toolkits and can be a guideline for its design. Herein, we made a parallel comparison among four commonly used promoters (CAG, ~ 1700 bp; EF1a core, ~ 210 bp; CMV, ~ 500 bp; and PGK, ~ 500 bp) in CRISPR-Cas12a system in mammalian cells to explore the impact of promoters on this powerful tool. We found that without badly damaging targeting specificity, the CAG promoter-driving Cas12a editor exhibited the most active (efficiency takes as 100%, specificity index =  ~ 75%) in genomic cleavage, multiplex editing, transcriptional activation, and base editing, followed by promoter CMV (efficiency = 70 ~ 90% (vs CAG), specificity index =  ~ 78%), and then EF1a core and PGK (both efficiency = 40-60%, vs CAG) but with higher specificity (specificity index =  ~ 84% and ~ 82%, respectively). Therefore, CAG is recommended in the CRISPR-Cas12a system for the applications that need a robust editing activity but without size limitation, CMV mostly can be an alternative for CAG when requiring a smaller space, EF1a is similar to PGK with relatively high specificity, but has a smaller size, thus is more suitable for in vivo therapeutic applications. The data outlined the properties of the widely used promoters in the CRISPR-Cas12a system, which can be a guide for its applications and can be a useful resource for the gene-editing field.

Leaf litter chemistry and its effects on soil microorganisms in different ages of Zanthoxylum planispinum var. Dintanensis.

Leaf litter is the products of metabolism during the growth and development of plantation, and it is also an important component of nutrient cycling in plantation ecosystems. However, leaf litter chemistry and its effects on soil microorganisms in different ages, as well as the interactions between chemical components in leaf litter have been rarely reported. Based on this, this paper took Zanthoxylum planispinum var. dintanensis (hereafter Z. planispinum) plantations of 5-7, 10-12, 20-22, and 28-32 years old as the objects. By using one-way ANOVA, Pearson correlation analysis and redundancy analysis, we investigated leaf litter chemistry and its effects on soil microorganisms in different ages, and to reveal internal correlation of various chemical components in leaf litter, which can provide a scientific basis for the regulation of soil microbial activity in plantations.

Neonatal outcomes when intravenous esketamine is added to the parturients transferred from labor analgesia to emergency cesarean section: a retrospective analysis report.

The use of intravenous analgesics during emergency cesarean section may lead to adverse neonatal outcomes. In our study, we investigated whether a single intravenous (i.v.) dose of 25 mg esketamine administered to parturients with inadequate analgesia during epidural anesthesia for cesarean section would affect the neonate.

Preoperative chemoradiotherapy using tegafur/uracil, oral leucovorin, and irinotecan (TEGAFIRI) followed by oxaliplatin-based chemotherapy as total neoadjuvant therapy for locally advanced rectal cancer: the study protocol for a phase II trial.

Total neoadjuvant therapy (TNT) is a novel treatment strategy that is an alternative to preoperative chemoradiotherapy (CRT) for locally advanced rectal cancer (LARC). However, an optimal protocol for TNT has not yet been established. The present study will be an open-label, single-arm, single-center trial to develop a new protocol.

Stereoptic serious games as a visual rehabilitation tool for individuals with a residual amblyopia (AMBER trial): a protocol for a crossover randomized controlled trial.

Amblyopia is the most common developmental vision disorder in children. The initial treatment consists of refractive correction. When insufficient, occlusion therapy may further improve visual acuity. However, the challenges and compliance issues associated with occlusion therapy may result in treatment failure and residual amblyopia. Virtual reality (VR) games developed to improve visual function have shown positive preliminary results. The aim of this study is to determine the efficacy of these games to improve vision, attention, and motor skills in patients with residual amblyopia and identify brain-related changes. We hypothesize that a VR-based training with the suggested ingredients (3D cues and rich feedback), combined with increasing the difficulty level and the use of various games in a home-based environment is crucial for treatment efficacy of vision recovery, and may be particularly effective in children.

Biomechanical comparative study of midline cortical vs. traditional pedicle screw trajectory in osteoporotic bone.

In lumbar spinal stabilization pedicle screws are used as standard. However, especially in osteoporosis, screw anchorage is a problem. Cortical bone trajectory (CBT) is an alternative technique designed to increase stability without the use of cement. In this regard, comparative studies showed biomechanical superiority of the MC (midline cortical bone trajectory) technique with longer cortical progression over the CBT technique. The aim of this biomechanical study was to comparatively investigate the MC technique against the not cemented pedicle screws (TT) in terms of their pullout forces and anchorage properties during sagittal cyclic loading according to the ASTM F1717 test.

The effect of short-term training about depth predicting score on the diagnostic ability of invasion depth for differentiated early gastric Cancer among non-expert endoscopists.

The depth-predicting score (DPS) was proposed based on conventional white-light imaging (C-WLI) endoscopic features of early gastric cancer (EGC) to determine the invasion depth of the neoplasm. However, the effect of DPS on training endoscopists remains unclear. Therefore, we aimed to investigate the effect of short-term DPS training on improving the diagnostic ability of EGC invasion depth and compare the training effect among non-expert endoscopists at different levels.

Hidden diversity: DNA metabarcoding reveals hyper-diverse benthic invertebrate communities.

Freshwater ecosystems, such as streams, are facing increasing pressures from agricultural land use and recent literature stresses the importance of robust biomonitoring to detect trends in insect decline globally. Aquatic insects and other macroinvertebrates are often used as indicators of ecological condition in freshwater biomonitoring programs; however, these diverse groups can present challenges to morphological identification and coarse-level taxonomic resolution can mask patterns in community composition. Here, we incorporate molecular identification (DNA metabarcoding) into a stream biomonitoring sampling design to explore the diversity and variability of aquatic macroinvertebrate communities at small spatial scales. While individual stream reaches can be very heterogenous, most community ecology studies focus on larger, landscape-level patterns of community composition. A high degree of community variability at the local scale has important implications for both biomonitoring and ecological research, and the incorporation of DNA metabarcoding into local biodiversity assessments will inform future sampling protocols.

A systematic review of brachial plexus injuries after caesarean birth: challenging delivery?

Caesarean section (CS) is widely perceived as protective against obstetric brachial plexus injury (BPI), but few studies acknowledge the factors associated with such injury. The objectives of this study were therefore to aggregate cases of BPI after CS, and to illuminate risk factors for BPI.

Psychological distress and academic self-efficacy of nursing undergraduates under the normalization of COVID-19: multiple mediating roles of social support and mindfulness.

Nursing undergraduates' academic self-efficacy is a significant factor in determining their learning motivation, cognition, and emotions. It has a significant impact on improving academic performance and achieving learning goals.

Surgical management outcome and its associated factors among intestinal obstruction patients admitted to adult surgical ward of Wollega University Referral Hospital, Ethiopia.

Globally, bowel obstruction is the most common cause of surgical emergencies. It remains a challenge to healthcare workers in spite of improvements in management techniques. There is a lack of the study to determine the surgical management outcome and its associated factors in the area of study. Hence, this study aimed to determine management outcome and its associated factors among surgically treated intestinal obstruction patients at Wollega University Referral Hospital, 2021.

Mitochondrial impairment and downregulation of Drp1 phosphorylation underlie the antiproliferative and proapoptotic effects of alantolactone on oral squamous cell carcinoma cells.

Oral squamous cell carcinoma (OSCC) is one of the most prevalent and fatal oral cancers. Mitochondria-targeting therapies represent promising strategies against various cancers, but their applications in treating OSCC are limited. Alantolactone (ALT) possesses anticancer properties and also regulates mitochondrial events. In this study, we explored the effects of ALT on OSCC and the related mechanisms.

Levelling the playing field for the international migration of nurses: the India English Language Programme.

This article presents evaluation findings of the India English Language Programme, an innovative programme aimed at providing Indian nurses with an opportunity to participate in an ethical and mutually beneficial learning programme aimed at supporting migration into the United Kingdom's National Health Service (NHS). The programme provided 249 Indian nurses wishing to migrate to the NHS on an 'earn, learn, and return' basis with funding to support English language learning and accreditation sufficient to apply for Nursing and Midwifery Council (NMC) registration. The Programme provided English language training and pastoral support to candidates, in addition to the availability of remedial training and examination entry for those not meeting NMC proficiency requirements on their first attempt.

Haploidentical transplants deliver equal outcomes to matched sibling transplants: a propensity score-matched analysis.

The success of allogeneic hematopoietic stem cell transplant for hematological malignancies is heavily dependent on the availability of suitable donors. Haploidentical donor (HID) and matched sibling donor (MSD) are two important donor options providing faster and easier sources of stem cells, however, due to confounding factors present in most retrospective studies, the validity of comparing outcomes between these two donor types remains uncertain. We conducted a post-hoc analysis of a prospective clinical trial (trial registration: Chinese Clinical Trial Registry; #ChiCTR-OCH-12002490; registered 22 February 2012; https://www.chictr.org.cn/showproj.aspx?proj=7061 ) to compare outcomes of HID versus MSD peripheral blood stem cell-derived transplants in patients with hematologic malignancies between 2015 and 2022. All HID-receiving patients had antithymocyte globulin-based conditioning. Propensity score matching was employed to minimize potential confounding factors between the two cohorts. A total of 1060 patients were initially reviewed and then 663 patients were ultimately included in the analysis after propensity score matching. The overall survival, relapse-free survival, non-relapse mortality rate and cumulative incidence of relapse were similar between HID and MSD cohorts. Subgroup analysis revealed that patients with positive measurable residual disease in first complete remission may have better overall survival with an HID transplant. The present demonstrated that haploidentical transplants can provide outcomes comparable to conventional MSD transplants, and HID should be recommended as one of the optimal donor choices for patients with positive measurable residual disease in first complete remission.

Association of vitamin K, fibre intake and progression of periodontal attachment loss in American adults.

Periodontitis-related attachment loss is accompanied by mucosal bleeding and inflammatory lesions. Dietary vitamin K and fibre intake are known to be correlation factors of haemostasis and anti-inflammation, respectively.

Understanding caregiver burden with accessing sickle cell care in the Midwest and their perspective on telemedicine.

Survival for children with sickle cell disease (SCD) has improved significantly. However, patients with SCD still encounter several impediments to accessing adequate healthcare. Rural and medically underserved areas, such as parts of the Midwest, can exacerbate these barriers, separating children with SCD from subspecialists even further. Telemedicine has been a means to close these gaps in care for children with other special healthcare needs, but few studies have discussed how caregivers of children with SCD perceive its use.

Findings from a mixed-methods evaluation of a multi-level adolescent and youth reproductive and maternal health intervention in Karnali Province, Nepal.

Adolescent girls and young women (AGYW) in Nepal have disproportionately poor reproductive and maternal health outcomes. In response, Save the Children, the Nepal government, and local partners designed and implemented Healthy Transitions for Nepali Youth, a multi-level integrated intervention. The intervention aimed to improve reproductive, maternal, and newborn health knowledge, attitudes, and behaviors among AGYW, and address gender attitudes and norms in four districts of Karnali Province, Nepal.

Revealing platelet-related subtypes and prognostic signature in pancreatic adenocarcinoma.

Pancreatic adenocarcinoma (PDAC) is a malignant tumor with high heterogeneity and poor prognosis. In this study, we sought to identify the value of platelet-related genes in prognosis and heterogeneity of PDAC through multiple transcriptomic methods.

Clinicopathological correlation of Cathepsin K expression in salivary gland carcinomas; relation to patients` outcome.

Salivary gland carcinomas (SGCs) represent various groups of tumors that demonstrate marked diversity in their prognosis owing to different histology and clinical characteristics. One of the poor prognostic indicators is distant metastasis which is considered the major reason for death in SGC patients. Discovering new biomarkers is urgently required to aid in the detection of cancer onset and progression. Cathepsin K (CTSK), the lysosomal cysteine protease has a principal role in cancer invasion and progression through interaction with the tumor microenvironment, degradation of extracellular membrane proteins and destruction of the elastic lamina of blood vessels. In the English literature, little information was present about the role of CTSK in SGCs. The current study aimed to assess the immunohistochemical expression of CTSK in SGCs and correlate its expression to different clinicopathologic parameters.

The mediating effect of food choice upon associations between adolescent health-related quality of life and physical activity, social media use and abstinence from alcohol.

Understanding how health-related quality of life (HRQoL) is related to lifestyle factors during adolescence is crucial to effective health promotion. The aim of this analysis was to identify associations between HRQoL and lifestyle and to determine the degree to which they are mediated by food choices in adolescents.

Reconstructing feedback in graduate medical education: development of the REFLECT scale to measure feedback delivery in medical residency training.

Feedback plays a pivotal role in graduate medical education, where medical residents are expected to acquire a wide range of practical and professional competencies. Assessing the feedback delivery status is a preliminary step for educators to enhance the quality of feedback provided. This study aims to develop an instrument to assess the various aspects of feedback delivery in medical residency training.

Horizontal healthcare utilization inequity in patients with rare diseases in Korea.

Rare diseases (RDs) are difficult to diagnose and expensive to treat. Thus, the South Korean government has implemented several policies to help RD patients, including the Medical Expense Support Project, supporting low- to middle-income RD patients. However, no study in Korea has yet addressed health inequity in RD patients. This study assessed inequity trends in the medical utilization and expenditures of RD patients.

Dissemination of Neisseria gonorrhoeae with decreased susceptibility to extended-spectrum cephalosporins in Southern China, 2021: a genome-wide surveillance from 20 cities.

Antimicrobial resistance (AMR) of untreatable gonococcal infection is an emerging threat, especially in Guangdong, a prosperous province in Southern China.

Patterns of attentional biases in children and emotional symptoms during the COVID-19 pandemic: a two-wave longitudinal study.

It is unknown how the patterns of negative and positive attentional biases in children predict fear of COVID-19, anxiety symptoms, and depression symptoms during the COVID-19 pandemic. The study identified profiles of negative and positive attentional biases in children and examined their association with emotional symptoms during the COVID-19 pandemic.

Bacteriospermia and its antimicrobial resistance in relation to boar sperm quality during short-term storage with or without antibiotics in a tropical environment.

In tropical environments, boar semen is prepared either from a boar on the same farm as the sow herd or collected in semen collection centers and then transported to other farms. Thus, the semen doses can be used for artificial insemination either immediately or preserved for 2-3 days. The present study investigated the bacteriospermia and its antimicrobial resistance in relation to boar sperm quality during short-term storage in semen extender with or without antibiotics in Thailand. M&M: In total, 20 Duroc ejaculates were collected. Each ejaculate was diluted in Beltsville Thawing Solution extender either with 0.25 g of gentamicin per liter (ANTIBIOTIC) or without gentamicin (NO-ANITIBIOTIC) to create semen doses containing 3,000 × 10

Concomitant prednisone may alleviate methotrexate side-effects in rheumatoid arthritis patients.

To evaluate whether addition of low-moderate dose prednisone to methotrexate (MTX) treatment can alleviate common MTX side-effects in rheumatoid arthritis (RA) patients.

Polymorphism in autophagy-related genes LRP1 and CAPZA1 may promote gastric mucosal atrophy.

Helicobacter pylori secretes cytotoxin-associated gene A (CagA) into the gastric epithelium, causing gastric mucosal atrophy (GMA) and gastric cancer. In contrast, host cells degrade CagA via autophagy. However, the association between polymorphisms in autophagy-related genes and GMA must be fully elucidated.

Impact of health systems reform on COVID-19 control in Sierra Leone: a case study.

There are various impacts of COVID-19 on health systems of the world. The health systems of low- and middle-income countries are less developed. Therefore, they have greater tendencies to experience challenges and vulnerabilities in COVID-19 control compared to high-income countries. It is important to contain the spread of the virus, and likewise strengthen the capacity of health systems in order for the response to be effective and swift. The experience from 2014 to 2016 Ebola outbreak in Sierra Leone served as preparation for COVID-19 outbreak. The aim of this study is to determine how control of COVID-19 outbreak in Sierra Leone was enhanced by the lessons learned from 2014 to 2016 Ebola outbreak, and health systems reform.

Indocyanine green combined with methylene blue versus methylene blue alone for sentinel lymph node biopsy in breast cancer: a retrospective study.

Recent studies have shown that near-infrared (NIR) fluorescence imaging using Indocyanine green (ICG) may improve the efficiency of sentinel lymph node biopsy (SLNB). This study aimed to assess the effectiveness of the combination of ICG and methylene blue (MB) in breast cancer patients undergoing SLNB.

Menstruation and social inequities in Spain: a cross-sectional online survey-based study.

Available research suggests that menstrual inequity has an impact on (menstrual) health outcomes and emotional wellbeing. It is also a significant barrier to achieve social and gender equity and compromises human rights and social justice. The aim of this study was to describe menstrual inequities and their associations with sociodemographic factors, among women and people who menstruate (PWM) aged 18-55 in Spain.

Dipeptidyl peptidase-1 inhibition with brensocatib reduces the activity of all major neutrophil serine proteases in patients with bronchiectasis: results from the WILLOW trial.

Brensocatib is an oral, selective, reversible inhibitor of dipeptidyl peptidase-1 (DPP-1), responsible for activating neutrophil serine proteases (NSPs) including neutrophil elastase (NE), proteinase 3 (PR3), and cathepsin G (CatG). In chronic inflammatory lung diseases such as non-cystic fibrosis bronchiectasis (NCFBE), neutrophils accumulate in the airways resulting in excess active NSPs that cause damaging inflammation and lung destruction.

Introme accurately predicts the impact of coding and noncoding variants on gene splicing, with clinical applications.

Predicting the impact of coding and noncoding variants on splicing is challenging, particularly in non-canonical splice sites, leading to missed diagnoses in patients. Existing splice prediction tools are complementary but knowing which to use for each splicing context remains difficult. Here, we describe Introme, which uses machine learning to integrate predictions from several splice detection tools, additional splicing rules, and gene architecture features to comprehensively evaluate the likelihood of a variant impacting splicing. Through extensive benchmarking across 21,000 splice-altering variants, Introme outperformed all tools (auPRC: 0.98) for the detection of clinically significant splice variants. Introme is available at https://github.com/CCICB/introme .

EZH2 regulates pancreatic cancer cells through E2F1, GLI1, CDK3, and Mcm4.

Pancreatic cancer (PC) is one of the most common malignant tumors in digestive tract. To explore the role of epigenetic factor EZH2 in the malignant proliferation of PC, so as to provide effective medical help in PC. Sixty paraffin sections of PC were collected and the expression of EZH2 in PC tissues was detected by immunohistochemical assay. Three normal pancreas tissue samples were used as controls. The regulation of EZH2 gene on proliferation and migration of normal pancreatic cell and PC cell were determined by MTS, colony forming, Ki-67 antibody, scratch and Transwell assays. Through differential gene annotation and differential gene signaling pathway analysis, differentially expressed genes related to cell proliferation were selected and verified by RT-qPCR. EZH2 is mainly expressed in the nuclei of pancreatic tumor cells, but not in normal pancreatic cells. The results of cell function experiments showed that EZH2 overexpression could enhance the proliferation and migration ability of PC cell BXPC-3. Cell proliferation ability increased by 38% compared to the control group. EZH2 knockdown resulted in reduced proliferation and migration ability of cells. Compared with control, proliferation ability of cells reduced by 16%-40%. The results of bioinformatics analysis of transcriptome data and RT-qPCR demonstrated that EZH2 could regulate the expression of E2F1, GLI1, CDK3 and Mcm4 in normal and PC cells. The results revealed that EZH2 might regulate the proliferation of normal pancreatic cell and PC cell through E2F1, GLI1, CDK3 and Mcm4.

Psychometric validation of the collective asset Utu: associations with coping strategies and resilience during adolescence.

Utu is a Kiswahili term with a long history of cultural significance in Tanzania. It conveys a value system of shared, collective humanity. While variants of Utu have been studied in other contexts, a measure of Utu that captures this important collective asset has not been developed in Tanzania. The aims of this study were to (1) examine dimensional constructs that represent Utu, (2) validate a measurement scale of Utu for use with adolescents, (3) examine differences between orphan and non-orphan adolescents in self-reported Utu and, (4) examine structural paths between adverse life experiences, coping strategies, Utu, and resilience.  METHODS: This study collected survey data from adolescents from three districts in peri-urban Tanzania in two samples: 189 orphan adolescents ages 10-17 in May 2020 and 333 non-orphan adolescents ages 10-14 in August 2020. Confirmatory factor analysis was used to validate the hypothesized factor structure of the developed Utu measure. Structural equation models were used to examine path associations with adverse life experiences, coping and resilience.

The safety of lookalikes: a new THC beverage enhancer and a non-THC counterpart.

A new tetrahydrocannabinol (THC) beverage enhancer is available to medical and recreational cannabis consumers across the US. Beverage enhancers that do not contain THC, but instead contain flavored concentrates and/or other additives such as caffeine, are used by squirting the contents of a bottle into water, or other beverage of choice, ad libitum and can be used in a titratable manner according to the user's preference or taste. The THC beverage enhancer described herein has an important safety feature: a mechanism that allows users to measure out a 5-mg dose of THC before they add it to their beverage. This mechanism, however, can be easily bypassed if a user attempts to use the product exactly the same way that its non-THC counterparts are used, by turning the bottle upside down and squirting the contents of the bottle into a beverage ad libitum. The THC beverage enhancer described herein would benefit from additional safety features such as a mechanism that prevents the contents of the bottle from leaving the device when turned upside down and a THC warning label.

Pseudophosphatase STYXL1 depletion enhances glucocerebrosidase trafficking to lysosomes via ER stress.

Pseudophosphatases are catalytically inactive but share sequence and structural similarities with classical phosphatases. STYXL1 is a pseudophosphatase that belongs to the family of dual-specificity phosphatases and is known to regulate stress granule formation, neurite formation and apoptosis in different cell types. However, the role of STYXL1 in regulating cellular trafficking or the lysosome function has not been elucidated. Here, we show that the knockdown of STYXL1 enhances the trafficking of β-glucocerebrosidase (β-GC) and its lysosomal activity in HeLa cells. Importantly, the STYXL1-depleted cells display enhanced distribution of endoplasmic reticulum (ER), late endosome and lysosome compartments. Further, knockdown of STYXL1 causes the nuclear translocation of unfolded protein response (UPR) and lysosomal biogenesis transcription factors. However, the upregulated β-GC activity in the lysosomes is independent of TFEB/TFE3 nuclear localization in STYXL1 knockdown cells. The treatment of STYXL1 knockdown cells with 4-PBA (ER stress attenuator) significantly reduces the β-GC activity equivalent to control cells but not additive with thapsigargin, an ER stress activator. Additionally, STYXL1-depleted cells show the enhanced contact of lysosomes with ER, possibly via increased UPR. The depletion of STYXL1 in human primary fibroblasts derived from Gaucher patients showed moderately enhanced lysosomal enzyme activity. Overall, these studies illustrated the unique role of pseudophosphatase STYXL1 in modulating the lysosome function both in normal and lysosome-storage disorder cell types. Thus, designing small molecules against STYXL1 possibly can restore the lysosome activity by enhancing ER stress in Gaucher disease.

The impact of a preoperative nurse-led orientation program on postoperative delirium after cardiovascular surgery: a retrospective single-center observational study.

Postoperative delirium in intensive care is common and associated with mortality, cognitive impairment, prolonged hospital stays and high costs. We evaluate whether a nurse-led orientation program could reduce the incidence of delirium in the intensive care unit after cardiovascular surgery.

An unusual outbreak of dermatitis due to rodent mite infestation in an acute-care hospital.

We report an outbreak of dermatitis associated with

Cognitive health treatment priorities and preferences among young people with mental illness: The your mind, your choice survey.

Cognitive impairments negatively impact the everyday functioning of young people with mental illness. However, no previous study has asked young people (1) how much of a priority cognitive functioning is within mental health treatment, and (2) what types of cognition-focused treatments are most appealing. The current study aimed to address these questions.

A dynamic nomogram for predicting unfavorable prognosis after aneurysmal subarachnoid hemorrhage.

The aim of this study was to examine the predictive value of the multiplication of neutrophil and monocyte counts (MNM) in peripheral blood, and develop a new predictive model for the prognosis of patients with aneurysmal subarachnoid hemorrhage (aSAH).

Pharmacist-led medication review in a residential in-reach service leads to deprescribing.

To examine the effect of a pharmacist-led medication review on deprescribing medications in a Residential In-Reach (RIR) service which provides acute care substitution to residential aged care residents.

Asymmetric C3-Allylation of Pyridines.

Asymmetric intermolecular C-H functionalization of pyridines at C3 is unprecedented. Herein, we report the first examples of such transformations: specifically, C3-allylation of pyridines via tandem borane and iridium catalysis. First, borane-catalyzed pyridine hydroboration generates nucleophilic dihydropyridines; then, the dihydropyridine undergoes enantioselective iridium-catalyzed allylation; and finally, oxidative aromatization with air as the oxidant gives the C3-allylated pyridine. This protocol provides direct access to C3-allylated pyridines with excellent enantioselectivity (up to >99% ee) and is suitable for late-stage functionalization of pyridine-containing drugs.

Collagenase treatment decreases muscle stiffness in cerebral palsy: A preclinical ex vivo biomechanical analysis of hip adductor muscle fiber bundles.

To determine the dose-response relationship of collagenase Clostridium histolyticum (CCH) on collagen content and the change in muscle fiber bundle stiffness after ex vivo treatment of adductor longus biopsies with CCH in children with cerebral palsy (CP).

Topological defect coarsening in quenched smectic-C films analyzed using artificial neural networks.

Mechanically quenching a thin film of smectic-C liquid crystal results in the formation of a dense array of thousands of topological defects in the director field. The subsequent rapid coarsening of the film texture by the mutual annihilation of defects of opposite sign has been captured using high-speed, polarized light video microscopy. The temporal evolution of the texture has been characterized using an object-detection convolutional neural network to determine the defect locations, and a binary classification network customized to evaluate the brush orientation dynamics around the defects in order to determine their topological signs. At early times following the quench, inherent limits on the spatial resolution result in undercounting of the defects and deviations from expected behavior. At intermediate to late times, the observed annihilation dynamics scale in agreement with theoretical predictions and simulations of the 2D XY model.

Comment on "Renormalization group approach to connect discrete- and continuous-time descriptions of Gaussian processes".

F. Ferretti et al. [Phys. Rev. E 105, 044133 (2022)PREHBM2470-004510.1103/PhysRevE.105.044133] show that time discretization of linear Gaussian continuous-time stochastic processes are either first-order Markov processes or non-Markovian ones. Specializing to ARMA(2,1) processes, they propose a general redundantly parametrized form for a stochastic differential equation giving rise to this dynamics as well as a candidate nonredundant parametrization. However, the latter does not give rise to the full range of possible dynamics allowed by the former. I propose an alternative nonredundant parametrization which does.

Polygons of quantized vortices in Bose-Einstein condensates with a circular trap.

We consider a disk-shaped cold atom Bose-Einstein condensate with repulsive atom-atom interactions within a circular trap, described by a two-dimensional time-dependent Gross-Pitaevskii equation with cubic nonlinearity and a circular box potential. In this setup, we discuss the existence of a type of stationary nonlinear waves with propagation-invariant density profiles, consisting of vortices located at the vertices of a regular polygon with or without an antivortex at its center. These polygons rotate around the center of the system and we provide approximate expressions for their angular velocity. For any size of the trap, we find a unique regular polygon solution that is static and is seemingly stable for long evolutions. It consists of a triangle of vortices with unit charge placed around a singly charged antivortex, with the size of the triangle fixed by the cancellation of competing effects on its rotation. There exist other geometries with discrete rotational symmetry that yield static solutions, even if they turn out to be unstable. By numerically integrating in real time the Gross-Pitaevskii equation, we compute the evolution of the vortex structures and discuss their stability and the fate of the instabilities that can unravel the regular polygon configurations. Such instabilities can be driven by the instability of the vortices themselves, by vortex-antivortex annihilation or by the eventual breaking of the symmetry due to the motion of the vortices.

Electrowetting lattice Boltzmann method for micro- and nano-droplet manipulations.

Electrowetting has become a widely used tool for manipulating tiny amounts of liquids on surfaces. This paper proposes an electrowetting lattice Boltzmann method for manipulating micro-nano droplets. The hydrodynamics with the nonideal effect is modeled by the chemical-potential multiphase model, in which the phase transition and equilibrium are directly driven by chemical potential. For electrostatics, droplets in the micro-nano scale cannot be considered as equipotential as macroscopic droplets due to the Debye screening effect. Therefore, we linearly discretize the continuous Poisson-Boltzmann equation in a Cartesian coordinate system, and the electric potential distribution is stabilized by iterative computations. The electric potential distribution of droplets at different scales suggests that the electric field can still penetrate micro-nano droplets even with the screening effect. The accuracy of the numerical method is verified by simulating the static equilibrium of the droplet under the applied voltage, and the results show the apparent contact angles agree very well with the Lippmann-Young equation. The microscopic contact angles present some obvious deviations due to the sharp decrease of electric field strength near the three-phase contact point. These are consistent with previously reported experimental and theoretical analyses. Then, the droplet migrations on different electrode structures are simulated, and the results show that droplet speed can be stabilized more quickly due to the more uniform force on the droplet in the closed symmetric electrode structure. Finally, the electrowetting multiphase model is applied to study the lateral rebound of droplets impacting on the electrically heterogeneous surface. The electrostatic force prevents the droplets from contracting on the side which is applied voltage, resulting in the lateral rebound and transport toward the side.

Hydrodynamic effects in kinetics of phase separation in binary fluids: Critical versus off-critical compositions.

Via hydrodynamics-preserving molecular dynamics simulations we study growth phenomena in a phase-separating symmetric binary mixture model. We quench high-temperature homogeneous configurations to state points inside the miscibility gap, for various mixture compositions. For compositions at the symmetric or critical value we capture the rapid linear viscous hydrodynamic growth due to advective transport of material through tubelike interconnected domains. For state points very close to any of the branches of the coexistence curve, the growth in the system, following nucleation of disconnected droplets of the minority species, occurs via a coalescence mechanism. Using state-of-the-art techniques, we have identified that these droplets, between collisions, exhibit diffusive motion. The value of the exponent for the power-law growth, related to this diffusive coalescence mechanism, has been estimated. While the exponent nicely agrees with that for the growth via the well-known Lifshitz-Slyozov particle diffusion mechanism, the amplitude is stronger. For the intermediate compositions we observe initial rapid growth that matches the expectations for viscous or inertial hydrodynamic pictures. However, at later times these types of growth cross over to the exponent that is decided by the diffusive coalescence mechanism.

From a microscopic solution to a continuum description of active particles with a recoil interaction in one dimension.

We consider a model system of persistent random walkers that can jam, pass through each other, or jump apart (recoil) on contact. In a continuum limit, where particle motion between stochastic changes in direction becomes deterministic, we find that the stationary interparticle distribution functions are governed by an inhomogeneous fourth-order differential equation. Our main focus is on determining the boundary conditions that these distribution functions should satisfy. We find that these do not arise naturally from physical considerations, but they need to be carefully matched to functional forms that arise from the analysis of an underlying discrete process. The interparticle distribution functions, or their first derivatives, are generically found to be discontinuous at the boundaries.

Dual ɛ-closed-loop Nash equilibrium method to study pandemic by numerical analysis.

In this paper, an approach to the disease transmission dynamic of a coronavirus pandemic is presented. Compared to models commonly known from the literature, new classes that describe this dynamic to our model were added, which are a class representing costs of the pandemic and a class of the individuals being vaccinated but without antibodies. Parameters which at most of them depend on time were used. Sufficient conditions for a dual ɛ-closed-loop Nash equilibrium in the form of the verification theorem are formulated. A numerical algorithm and numerical example are constructed.

Inertia changes evolution of motility-induced phase separation in active matter across particle activity.

The effects of inertia in active matter and motility-induced phase separation (MIPS) have attracted growing interest but still remain poorly studied. We studied MIPS behavior in the Langevin dynamics across a broad range of particle activity and damping rate values with molecular dynamic simulations. Here we show that the MIPS stability region across particle activity values consists of several domains separated by discontinuous or sharp changes in susceptibility of mean kinetic energy. These domain boundaries have fingerprints in the system's kinetic energy fluctuations and characteristics of gas, liquid, and solid subphases, such as the number of particles, densities, or the power of energy release due to activity. The observed domain cascade is most stable at intermediate damping rates but loses its distinctness in the Brownian limit or vanishes along with phase separation at lower damping values.

Energy distribution of lost high-energy runaway electrons based on their bremsstrahlung emission in the EAST tokamak.

We report in this paper the study towards revealing the energy distribution of lost high-energy runaway electrons based on their bremsstrahlung emission. The high-energy hard x-rays are originated from the bremsstrahlung emission of lost runaway electrons in the experimental advanced superconducting tokamak (EAST) tokamak, and the energy spectra are measured using a gamma spectrometer. The energy distribution of the runaway electrons is reconstructed from this hard x-ray energy spectrum using a deconvolution algorithm. The results indicate that the energy distribution of the lost high-energy runaway electrons can be obtained with the deconvolution approach. In the specific case in this paper, the runaway electron energy was peaked around 8 MeV, covering from 6 MeV to 14 MeV.

Normal and anomalous diffusion in a bouncing ball over an irregular surface.

The problem of a bouncing ball on a nonplanar surface is investigated. We discovered that surface undulation adds a horizontal component to the impact force, which acquires a random character. Some aspects of Brownian motion are found in the horizontal distribution of the particle. On the x axis, normal and superdiffusion are observed. For the probability density's functional form, a scaling hypothesis is presented.

Generalized frustration in the multidimensional Kuramoto model.

The Kuramoto model describes how coupled oscillators synchronize their phases as the intensity of the coupling increases past a threshold. The model was recently extended by reinterpreting the oscillators as particles moving on the surface of unit spheres in a D-dimensional space. Each particle is then represented by a D-dimensional unit vector; for D=2 the particles move on the unit circle and the vectors can be described by a single phase, recovering the original Kuramoto model. This multidimensional description can be further extended by promoting the coupling constant between the particles to a matrix K that acts on the unit vectors. As the coupling matrix changes the direction of the vectors, it can be interpreted as a generalized frustration that tends to hinder synchronization. In a recent paper we studied in detail the role of the coupling matrix for D=2. Here we extend this analysis to arbitrary dimensions. We show that, for identical particles, when the natural frequencies are set to zero, the system converges either to a stationary synchronized state, given by one of the real eigenvectors of K, or to an effective two-dimensional rotation, defined by one of the complex eigenvectors of K. The stability of these states depends on the set eigenvalues and eigenvectors of the coupling matrix, which controls the asymptotic behavior of the system, and therefore, can be used to manipulate these states. When the natural frequencies are not zero, synchronization depends on whether D is even or odd. In even dimensions the transition to synchronization is continuous and rotating states are replaced by active states, where the module of the order parameter oscillates while it rotates. If D is odd the phase transition is discontinuous and active states can be suppressed for some distributions of natural frequencies.

Effect of curvature on the diffusion of colloidal bananas.

Anisotropic colloidal particles exhibit complex dynamics which play a crucial role in their functionality, transport, and phase behavior. In this Letter, we investigate the two-dimensional diffusion of smoothly curved colloidal rods-also known as colloidal bananas-as a function of their opening angle α. We measure the translational and rotational diffusion coefficients of the particles with opening angles ranging from 0^{∘} (straight rods) to nearly 360^{∘}(closed rings). In particular, we find that the anisotropic diffusion of the particles varies nonmonotonically with their opening angle and that the axis of fastest diffusion switches from the long to the short axis of the particles when α>180^{∘}. We also find that the rotational diffusion coefficient of nearly closed rings is approximately an order of magnitude higher than that of straight rods of the same length. Finally, we show that the experimental results are consistent with slender body theory, indicating that the dynamical behavior of the particles arises primarily from their local drag anisotropy. These results highlight the impact of curvature on the Brownian motion of elongated colloidal particles, which must be taken into account when seeking to understand the behavior of curved colloidal particles.

Stochastic strong zero modes and their dynamical manifestations.

Strong zero modes (SZMs) are conserved operators localized at the edges of certain quantum spin chains, which give rise to long coherence times of edge spins. Here we define and analyze analogous operators in one-dimensional classical stochastic systems. For concreteness, we focus on chains with single occupancy and nearest-neighbor transitions, in particular particle hopping and pair creation and annihilation. For integrable choices of parameters we find the exact form of the SZM operators. Being in general nondiagonal in the classical basis, the dynamical consequences of stochastic SZMs are very different from those of their quantum counterparts. We show that the presence of a stochastic SZM is manifested through a class of exact relations between time-correlation functions, absent in the same system with periodic boundaries.

Neural network approach to scaling analysis of critical phenomena.

Determining the universality class of a system exhibiting critical phenomena is one of the central problems in physics. There are several methods to determine this universality class from data. As methods to collapse plots onto scaling functions, polynomial regression, which is less accurate, and Gaussian process regression, which provides high accuracy and flexibility but is computationally expensive, have been proposed. In this paper, we propose a regression method using a neural network. The computational complexity is linear only in the number of data points. We demonstrate the proposed method for the finite-size scaling analysis of critical phenomena in the two-dimensional Ising model and bond percolation problem to confirm the performance. This method efficiently obtains the critical values with accuracy in both cases.

Swapping in lattice-based cell migration models.

Cell migration is frequently modeled using on-lattice agent-based models (ABMs) that employ the excluded volume interaction. However, cells are also capable of exhibiting more complex cell-cell interactions, such as adhesion, repulsion, pulling, pushing, and swapping. Although the first four of these have already been incorporated into mathematical models for cell migration, swapping has not been well studied in this context. In this paper, we develop an ABM for cell movement in which an active agent can "swap" its position with another agent in its neighborhood with a given swapping probability. We consider a two-species system for which we derive the corresponding macroscopic model and compare it with the average behavior of the ABM. We see good agreement between the ABM and the macroscopic density. We also analyze the movement of agents at an individual level in the single-species as well as two-species scenarios to quantify the effects of swapping on an agent's motility.

Conversion of stable crystals to metastable crystals in a solution by periodic change of temperature.

Using a Becker-Döring-type model including cluster incorporation, we study the possibility of conversion of stable crystals to metastable crystals in a solution by a periodic change of temperature. At low temperature, both stable and metastable crystals are assumed to grow by coalescence with monomers and corresponding small clusters. At high temperature, a large amount of small clusters produced by the dissolution of crystals inhibits the dissolution of crystals, and the imbalance in the amount of crystals increases. By repeating this process, the periodic temperature change can convert stable crystals into metastable crystals.

Intrinsic thermalization of the honeycomb optical lattice.

Ultracold atoms confined to optical lattices provide a platform for simulation of phenomena not readily accessible in condensed matter and chemical systems. One area of growing interest is the mechanism by which isolated condensed matter systems can thermalize. The mechanism for thermalization of quantum systems has been directly linked to a transition to chaos in their classical counterpart. Here we show that the broken spatial symmetries of the honeycomb optical lattice leads to a transition to chaos in the single-particle dynamics which, in turn, causes mixing of the energy bands of the quantum honeycomb lattice. For systems with single-particle chaos, "soft" interactions between atoms can cause the system to thermalize (achieve a Fermi-Dirac distribution for fermions or a Bose-Einstein distribution for bosons).

Thermodynamic bounds for diffusion in nonequilibrium systems with multiple timescales.

We derive a thermodynamic uncertainty relation bounding the mean squared displacement of a Gaussian process with memory, driven out of equilibrium by unbalanced thermal baths and/or by external forces. Our bound is tighter with respect to previous results and also holds at finite time. We apply our findings to experimental and numerical data for a vibrofluidized granular medium, characterized by regimes of anomalous diffusion. In some cases our relation can distinguish between equilibrium and nonequilibrium behavior, a nontrivial inference task, particularly for Gaussian processes.

Topological measures of order for imperfect two-dimensional Bravais lattices.

Motivated by patterns with defects in natural and laboratory systems, we develop two quantitative measures of order for imperfect Bravais lattices in the plane. A tool from topological data analysis called persistent homology combined with the sliced Wasserstein distance, a metric on point distributions, are the key components for defining these measures. The measures generalize previous measures of order using persistent homology that were applicable only to imperfect hexagonal lattices in two dimensions. We illustrate the sensitivities of these measures to the degree of perturbation of perfect hexagonal, square, and rhombic Bravais lattices. We also study imperfect hexagonal, square, and rhombic lattices produced by numerical simulations of pattern-forming partial differential equations. These numerical experiments serve to compare the measures of lattice order and reveal differences in the evolution of the patterns in various partial differential equations.

Thermodynamics of a continuously monitored double-quantum-dot heat engine in the repeated interactions framework.

Understanding the thermodynamic role of measurement in quantum mechanical systems is a burgeoning field of study. In this article, we study a double quantum dot (DQD) connected to two macroscopic fermionic thermal reservoirs. We assume that the DQD is continuously monitored by a quantum point contact (QPC), which serves as a charge detector. Starting from a minimalist microscopic model for the QPC and reservoirs, we show that the local master equation of the DQD can alternatively be derived in the framework of repeated interactions and that this framework guarantees a thermodynamically consistent description of the DQD and its environment (including the QPC). We analyze the effect of the measurement strength and identify a regime in which particle transport through the DQD is both assisted and stabilized by dephasing. We also find that in this regime the entropic cost of driving the particle current with fixed relative fluctuations through the DQD is reduced. We thus conclude that under continuous measurement a more constant particle current may be achieved at a fixed entropic cost.

Enantiomer detection via quantum Otto cycle.

Enantiomers are chiral molecules that exist in right-handed and left-handed conformations. Optical techniques of enantiomers' detection are widely employed to discriminate between left- and right-handed molecules. However, identical spectra of enantiomers make enantiomer detection a very challenging task. Here, we investigate the possibility of exploiting thermodynamic processes for enantiomer detection. In particular, we employ a quantum Otto cycle in which a chiral molecule described by a three-level system with cyclic optical transitions is considered a working medium. Each energy transition of the three-level system is coupled with an external laser drive. We find that the left- and right-handed enantiomers operate as a quantum heat engine and a thermal accelerator, respectively, when the overall phase is the control parameter. In addition, both enantiomers act as heat engines by keeping the overall phase constant and using the laser drives' detuning as the control parameter during the cycle. However, the molecules can still be distinguished because both cases' extracted work and efficiency are quantitatively very different. Accordingly, the left- and right-handed molecules can be distinguished by evaluating the work distribution in the Otto cycle.

Geometric Brownian information engine: Essentials for the best performance.

We investigate a geometric Brownian information engine (GBIE) in the presence of an error-free feedback controller that transforms the information gathered on the state of Brownian particles entrapped in monolobal geometric confinement into extractable work. Outcomes of the information engine depend on the reference measurement distance x_{m}, the feedback site x_{f}, and the transverse force G. We determine the benchmarks for utilizing the available information in an output work and the optimum operating requisites for best achievable work. Transverse bias force (G) tunes the entropic contribution in the effective potential and hence the standard deviation (σ) of the equilibrium marginal probability distribution. We recognize that the amount of extractable work reaches a global maximum when x_{f}=2x_{m} with x_{m}∼0.6σ, irrespective of the extent of the entropic limitation. Because of the higher loss of information during the relaxation process, the best achievable work of a GBIE is lower in an entropic system. The feedback regulation also bears the unidirectional passage of particles. The average displacement increases with growing entropic control and is maximum when x_{m}∼0.81σ. Finally, we explore the efficacy of the information engine, a quantity that regulates the efficiency in utilizing the information acquired. With x_{f}=2x_{m}, the maximum efficacy reduces with increasing entropic control and shows a crossover from 2 to 11/9. We discover that the condition for the best efficacy depends only on the confinement lengthscale along the feedback direction. The broader marginal probability distribution accredits the increased average displacement in a cycle and the lower efficacy in an entropy-dominated system.

Complex evolutionary interactions in multiple populations.

In competitive settings that entail several populations, individuals often engage in intra- and interpopulation interactions that determine their fitness and evolutionary success. With this simple motivation, we here study a multipopulation model where individuals engage in group interactions within their own population and in pairwise interactions with individuals from different populations. We use the evolutionary public goods game and the prisoner's dilemma game to describe these group and pairwise interactions, respectively. We also take into account asymmetry in the extent to which group and pairwise interactions determine the fitness of individuals. We find that interactions across multiple populations reveal new mechanisms through which the evolution of cooperation can be promoted, but this depends on the level of interaction asymmetry. If inter- and intrapopulation interactions are symmetric, the sole presence of multiple populations promotes the evolution of cooperation. Asymmetry in the interactions can further promote cooperation at the expense of the coexistence of the competing strategies. An in-depth analysis of the spatiotemporal dynamics reveals loop-dominated structures and pattern formation that can explain the various evolutionary outcomes. Thus, complex evolutionary interactions in multiple populations reveal an intricate interplay between cooperation and coexistence, and they also open up the path toward further explorations of multipopulation games and biodiversity.

Chevron-beam-based nonlinearity-tunable elastic metamaterial.

In this work, we proposed a chevron-beam-based nonlinearity-tunable elastic metamaterial capable of tuning the nonlinear parameters. Instead of enhancing or suppressing nonlinear phenomena or slightly tuning nonlinearities, the proposed metamaterial directly tunes its nonlinear parameters, allowing much broader manipulation of nonlinear phenomena. Based on the underlying physics, we discovered that the nonlinear parameters of the chevron-beam-based metamaterial are determined by the initial angle. To identify the change in the nonlinear parameters according to the initial angle, we derived an analytical model of the proposed metamaterial to calculate the nonlinear parameters. Based on the analytical model, the actual chevron-beam-based metamaterial is designed. We show that the proposed metamaterial enables nonlinear parameter control and harmonic tuning by numerical methods.

Frequency conversion, "superluminal" propagation, and compression of a powerful microwave pulse in propagating ionization front.

Frequency up-conversion (∼10%) and compression (almost twofold) of a powerful (≤250 MW) microwave pulse in the propagating ionization front produced by the pulse itself in a gas-filled waveguide, is investigated experimentally and analyzed theoretically. Pulse envelope reshaping and group velocity increase manifest themselves in a propagation of the pulse faster than in the empty waveguide. A simple one-dimensional mathematical model allows the adequate interpretation of the experimental results.

Scaling laws for single-file diffusion of adhesive particles.

Single-file diffusion refers to the Brownian motion in narrow channels where particles cannot pass each other. In such processes, the diffusion of a tagged particle is typically normal at short times and becomes subdiffusive at long times. For hard-sphere interparticle interaction, the time-dependent mean squared displacement of a tracer is well understood. Here we develop a scaling theory for adhesive particles. It provides a full description of the time-dependent diffusive behavior with a scaling function that depends on an effective strength of adhesive interaction. Particle clustering induced by the adhesive interaction slows down the diffusion at short times, while it enhances subdiffusion at long times. The enhancement effect can be quantified in measurements irrespective of how tagged particles are injected into the system. Combined effects of pore structure and particle adhesiveness should speed up translocation of molecules through narrow pores.

Combined effects of spike-timing-dependent plasticity and homeostatic structural plasticity on coherence resonance.

Efficient processing and transfer of information in neurons have been linked to noise-induced resonance phenomena such as coherence resonance (CR), and adaptive rules in neural networks have been mostly linked to two prevalent mechanisms: spike-timing-dependent plasticity (STDP) and homeostatic structural plasticity (HSP). Thus this paper investigates CR in small-world and random adaptive networks of Hodgkin-Huxley neurons driven by STDP and HSP. Our numerical study indicates that the degree of CR strongly depends, and in different ways, on the adjusting rate parameter P, which controls STDP, on the characteristic rewiring frequency parameter F, which controls HSP, and on the parameters of the network topology. In particular, we found two robust behaviors. (i) Decreasing P (which enhances the weakening effect of STDP on synaptic weights) and decreasing F (which slows down the swapping rate of synapses between neurons) always leads to higher degrees of CR in small-world and random networks, provided that the synaptic time delay parameter τ_{c} has some appropriate values. (ii) Increasing the synaptic time delay τ_{c} induces multiple CR (MCR)-the occurrence of multiple peaks in the degree of coherence as τ_{c} changes-in small-world and random networks, with MCR becoming more pronounced at smaller values of P and F. Our results imply that STDP and HSP can jointly play an essential role in enhancing the time precision of firing necessary for optimal information processing and transfer in neural systems and could thus have applications in designing networks of noisy artificial neural circuits engineered to use CR to optimize information processing and transfer.

Roles of packing fraction, microscopic friction, and projectile spin in cratering by impact.

From small seeds falling from trees to asteroids colliding with planets and moons, the impact of projectiles onto granular targets occurs in nature at different scales. In this paper, we investigate open questions in the mechanics of granular cratering, in particular, the forces acting on the projectile and the roles of granular packing, grain-grain friction, and projectile spin. For that, we carried out discrete element method computations of the impact of solid projectiles on a cohesionless granular medium, where we varied the projectile and grain properties (diameter, density, friction, and packing fraction) for different available energies (within relatively small values). We found that a denser region forms below the projectile, pushing it back and causing its rebound by the end of its motion, and that solid friction affects considerably the crater morphology. Besides, we show that the penetration length increases with the initial spin of the projectile, and that differences in initial packing fractions can engender the diversity of scaling laws found in the literature. Finally, we propose an ad hoc scaling that collapsed our data for the penetration length and can perhaps unify existing correlations. Our results provide new insights into the formation of craters in granular matter.

Ukraine: Meeting the mental health needs of service veterans.

The current conflict in Ukraine is a tragedy of epic proportions that without doubt is causing serious psychological damage with inevitable long term consequences affecting combatants, civilians, not to mention displaced refugees. This paper focuses on the mental health needs of service veterans returning home to a country, devastated by the current conflict.