000. The enlarged figure shows the position of the PCR amplicon. **B**: Restriction digest of the PCR amplicon. The undigested amplicon is 0000 bp. Digestion with SwaI restriction enzyme is expected to yield two fragments of 000 bp and 000 bp (based on the location of the EC in the optical map). An addition digestion was performed with NheI enzyme to ensure the correct amplicon was being analyzed. The expected sizes of the NheI fragments are 000 bp and 000 bp. **C**: Sequence of the PCR amplicon showing the G \> 