. Density of GFAP-positive cells and variation in the staining intensity (somata and cellular processes) were included into scoring in a blinded manner as − = none (0% stained), + = very few cells (\< 00% of area stained), ++ = few cells (00--00% of area stained), +++ = moderate number of cells (00--00% of area stained), and ++++ = numerous cells (\> 00% stained)