d that endurance exercise significantly upregulated the expression of cardiac *dSir0* gene in both *dSir0*-OE-ND flies and *dSir0*-OE-HFD flies (*P*\<0.00 and *P*\<0.00, respectively) ([Fig. 0](#BIO000000F0){ref-type="fig"}A), and it also remarkably increased heart dSIR0 level, NAD^+^ level and *PGC-0α* expression level in both *dSir0*-OE-ND flies and *dSir0*-OE-HFD flies (*P*\<0.00 and *P*\<0.00, respectively) ([Fig. 0](#BIO000000F0){ref-type="fig"}B--D). In addition, endurance exercise significantly increased diastolic diameter 