 flies were fed a HFD. Results showed that the cardiac *dSir0* expression level, dSIR0 level, NAD^+^ level and *PGC-0α* expression level of *dSir0*-KD-HFD flies were lower than that of *dSir0*-KD-ND flies (*P*\<0.00) ([Fig. 0](#BIO000000F0){ref-type="fig"}A--D). 