 by an initially denaturation for 00 min at 00°C, followed by 0 touch-down cycles at 00°C for 00 s, 00°C (-0°C per cycle) for 00 s, 00°C for 00 s, followed by 00 standard cycles (00°C for 00 s, 00°C for 00 s and 00°C for 00 s.) and a final extension at 00°C for 00 min. 