) Percentage of CD0^+^CSP^+^ spleen cells stained using PE-labeled anti-CD0 and APC-labeled MHC-I-pentamer loaded with CSP-peptide to detect CSP-specific CD0^+^ T cells. The mean of five immunized F0 DEREG mouse or a F0 non-transgenic littermates is shown. The percentage of CD0^+^CSP^+^ T cells is expressed as mean +**/−** SD of whole lymphocytes. \*\*, p\<0.00.](pone.0000000.g000){#pone-0000000-g000}  The