cruzi* infection. TSP-0KO MHEC grown on coverslips coated with 0% gelatin in 0 well culture plates were challenged with *T*. *cruzi* at different time points, washed, fixed, perforated with 0.0% Triton-X000, blocked with 0% BSA-PBS, and incubated at 0 °C overnight, in solutions containing phalloidin and (**A**) mouse anti YAP antibodies. The cells were washed and reprobed with goat anti mouse Alexa Fluor 000 conjugated secondary antibody. The washed cells were mounted with mounting media containing DAPI to stain the nuclei. (**B**) Stained slides were analyzed by confocal microscopy and the mean nuclear fluorescence intensity (MNFI) values were plotted for YAP. Images were captured at 00× at scale bar 00 μm. Each confocal microscopy image is a representative of three independent biological replicates. The bar graphs represent MNFI values ± SE from three independent biological replicates. The value of *p \<* 0.00 was considered 