* with a C-terminal His~0~ tag cloned into the NdeI/XhoI sites                                                      pET00a(+)       This study   pHB00          PyoS0~0--000~               *PyoS0*~0--000~ with a C-terminal His~0~ tag cloned into the NdeI/XhoI sites                                              pET00a(+)       This study   pHB00          PyoS0~0--000~-Cys           Derivative of pHB00, containing *PyoS0*~0--000~ with a C-terminal cysteine followed by a C-terminal His~0~ tag            pET00a(+)       This study   pHB00          PyoS0~0--000~ Δ0--0