 gap opening penalty of 0 and gap extension penalty of 0.0 for the multiple alignment and the Gonnet protein weight matrix. The phylogenetic tree was generated within MEGA 0 using the neighbour-joining method and the Jones-Taylor-Thornton substitution model. The bootstrap test was done using 0000 replications. The sequence of bovine α-chymotrypsinogen (NCBI accession number: P00000) w