  Reviewer \#0:  This study demonstrates that p00 binding to Cortactin promotes PAK0 interactions with Cortactin, and invadopodia disassembly by phosphorylation of Cortactin on Ser-000. In general, the biochemical analysis is strong and convincing. Indeed, the demonstration that a phosphomimetic mutant of Cortactin can complement the p00 deficiency phenotype on invadopodia disassembly is very interesting. However, there are a number of areas that require strengthening.  0\) The siRNA studies (Rac and RhoA) were performed with a single oligonucleotide. To exclude off0target effects, phenotypes should be confirmed with two independent siRNAs.  