 0\) One concern of the study is whether the cancer-associated mutants in eIF0A are applicable to human eIF0A function and cancer. The eIF0A-NTT is highly conserved between two species, but the Kozak consensus and dependence on it are generally thought to vary between yeast and humans. The study, therefore, provides new insight into how the eIF0A-NTT maintains the fidelity of start codon selection, but the possible role of the cancer-associated residues in human eIF0A in mammalian start site selection is not determined. Ideally, this should be addressed directly, but at a minimum the end of the title should be altered to include \"in yeast\" to better describe the study.  0\) They must have become aware of a very relevant paper Johnson et al. PLoS One. 0000 Jun 0;00(0):e0000000). The authors did RNAseq of polysome fractions prepared from actual μm cancer cells harboring the eIF0A-NTT mutation. They identified genes whose translational efficiency was affected by the knockdown of EIF0AX. Could the authors look for relevant information regarding mRNAs from the list? The paper should be referenced and its implications discussed.  0\) [Figure 0](#fig0){ref-type="fig"} convincingly demonstrates that the R00P and K00D mutations in eIF0A destabilize the closed/Pin conformation of the 00S PIC at UUG codons in vitro. Could they extend these results by showing a decreased UUG/AUG start codon usage in a cell-free translation system from eIF0A-mutant cells as compared to wildtype?  00.0000/eLife.00000.000  Author response  > Essential revisions: > > 0\) One concern of the study is whether the cancer-associated mutants in eIF0A are applicable to human eIF0A function and cancer. The eIF0A-NTT is highly conserved between two species, but the Kozak consensus and dependence on it are generally thought to vary between yeast and humans. The study, therefore, provides new insight into how the eIF0A-NTT maintains the fidelity of start codon selection, but the possible role of the cancer-associated residues in human eIF0A in mammalian start site selection is not determined. Ideally, this should be addressed directly, but at a minimum the end of the title should be altered to include \"in yeast\" to better describe the study.  