-HFD+E. Independent sample *t*-test was used to identify differences between *dSir0*-control flies and *dSir0*-OE flies. A two-way ANOVA was used to identify differences among the ND, ND+E, HFD, and HFD+E groups in *dSir0*-OE flies. Data are represented as means±s.e.m. \**P*\<0.00; \*\**P*\<0.00. The sample size was the same as with *w^0000^*.  