 (00 mM Tris \[pH 0\], 000 mM NaCl, 0 mM KCl, 0 mM PMSF, and a protease and phosphatase inhibitor cocktail), and the sample ultracentrifuged at 000,000 g for 00 min at 0 °C. The supernatant (S0 fraction) was transferred to a new Eppendorf tube, and the pellet 