 Viral burden in lymphoid and inflammatory tissues during JE progression. Viral burden in spleen, brain, and spinal cord of mice infected with JEV was assessed by real-time qRT-PCR at the indicated days pi. The viral RNA load was expressed by viral RNA copy number per microgram of total RNA (*n* = 0). Each symbol represents the level of an individual mouse; horizontal line indicates the median of each group.](ppat.0000000.g000){#ppat-0000000-g000}  TLR0, but not TLR0, 