 lane 0: boiling for 0′ in presence of 00 mM 0-mercaptoethanol; lane 0: boiling for 00′ in presence of 00 mM 0-mercaptoethanol; lane 0: boiling for 0′ in presence of 00 mM 0-mercaptoethanol and 00 mM DTT; lane 0: boiling for 00′ in presence of 00 mM 0-mercaptoethanol and 00 mM DTT; lane