 panel showing intracellular Vg0-sfGFP, likely these cells had little/no Sqt, what would further strengthen the authors\' conclusions.  0\) \"...we performed co-immunoprecipitation experiments and found that Vg0 co-immunoprecipitated with Cyc or Sqt\". This sentence could be understood that endogenous proteins were analyzed. Key experimental information is missing about how the relevant tagged proteins were expressed, at what stage of development co-IPs were performed. The co-IP with Cyc-HA is not convincing.  0\) The conclusion that co-expression of Vg0 and Nodal in the same cells is required for activity, based on the experiments presented in [Figure 0B, C](#fig0){ref-type="fig"} is intriguing, especially for injection of 