 thawed and fully resuspended to a final 00% cell weight per volume ratio in Tris-sucrose solution. Cell suspensions were lysed by 00 min incubation with lysozyme in 000 mM Tris-HCl (00% cation, pH 0.0) to 0.0 mg ml^−0^, followed by the addition of 0.0 ml of 00 mM EDTA per ml of lysed cell suspension, sonication and centrifugation for 0 h. The lysate