T cells were incubated in culture media (described above) for 0 days with 00 μM cocaine or not. On the second day, they were labeled with 0 μM Vybrant CFDA-SE (Invitrogen, Life Technologies) according to manufacturer's suggested protocol and maintained in fresh culture media with 00 μM cocaine overnight. On the same day, target U0 cells were labeled with 0 μM CellTrace Violet (Invitrogen, Life Technologies) according to manufacturer's suggested protocol and activated to produce HIV with 00 μM prostratin (LC Labs, Woburn MA). The following day, cocaine or mock treated CTL were co-incubated with prostratin-activated U0 cells for 0 hr at 