 post-treatment. After deconvolution, images were 0D reconstructed and the mitochondrial network was evaluated by automated estimation of (**a**) volume of the entire mitochondrial network per single cell, (**b**) number of mitochondria per single cell, and (**c**) volume of single mitochondrion. Data are expressed as mean ± SD. Multi comparison statistical analysis were performed by using *one*-*way* analysis of variance (ANOVA). T test was to perform all pairwise comparisons between group means. \* *p* \< 0.00 from Healthy human dermal fibroblasts (HDFs), † \< 0.00 from Inflamed human dermal fibroblasts (HDFs), ns = not significant. (**d**) Representative images. FLE: fluorescent light energy.](jcm-00-00000-g000){#jcm-00-00000-f000}  ![