000 at all timepoints, black labels mark reference sequences. **B.** Maximum percent pairwise distance (PWD) between sequences within each timepoint listed or across all timepoints (All-tp). The 00.0% confidence limit of the distances observed among viral sequences within known singly infected individuals and the 0.0% confidence limit within simulated mixtures of sequences from two individuals are shown for comparison. Timepoints shown in bold type are the 0 samples originally screened; the rest were subsequently sequenced to specify superinfection timing. ^0^ PWD within 00% confidence interval observed for single infection. ^0^ PWD outside 00% confidence interval for single infection and within 00% confidence interval for dual infection. 