 was added after blocking and retinas were incubated for 0--0 days under constant gentle shaking at room temperature. Primary antibodies were rabbit anti-GFP (Invitrogen, A-00000, 0:000) and goat anti-ChAT (Chemicon, Ab000P, 0:000). They were prepared in 0% NDS, 0% BSA, 0.0% TritonX-000, 0.00% NaN~0~ in 0x PBS. After incubation, retinas were washed three times for 00 min in 0x PBS with 0.0% TritonX-000 before being transferred into the secondary antibody solution (Alexa000 donkey anti-rabbit (Invitrogen, A00000, 0:000) and Alexa000 donkey anti-goat (Invitrogen A-00000, 0:000); prepared in 0% NDS, 0% BSA, 0.0% TritonX-000, 0.00% NaN~0~ in 0x PBS). Nuclei were stained with DAPI (Roche, 00000000000, 0:000) together with the secondary antibody solution. 