Glucose competence of the hepatoportal vein sensor requires the presence of an activated glucagon-like peptide-1 receptor. 
Activation of the hepatoportal glucose sensors by portal glucose infusion leads to increased glucose clearance and induction of hypoglycemia.  Here, we investigated whether glucagon-like peptide-1 (GLP-1) could modulate the activity of these sensors.  Mice were therefore infused with saline (S-mice) or glucose (P-mice) through the portal vein at a rate of 25 mg/kg.  min.  In P-mice, glucose clearance increased to 67.5 +/- 3.7 mg/kg.  min as compared with 24.1 +/- 1.5 mg/kg.  min in S-mice, and glycemia decreased from 5.0 +/- 0.1 to 3.3 +/- 0.1 mmol/l at the end of the 3-h infusion period.  Coinfusion of GLP-1 with glucose into the portal vein at a rate of 5 pmol/kg.  min (P-GLP-1 mice) did not increase the glucose clearance rate (57.4 +/- 5.0 ml/kg.  min) and hypoglycemia (3.8 +/- 0.1 mmol/l) observed in P-mice.  In contrast, coinfusion of glucose and the GLP-1 receptor antagonist exendin-(9-39) into the portal vein at a rate of 0.5 pmol/kg.  min (P-Ex mice) reduced glucose clearance to 36.1 +/- 2.6 ml/kg.  min and transiently increased glycemia to 9.2 +/- 0.3 mmol/l at 60 min of infusion before it returned to the fasting level (5.6 +/- 0.3 mmol/l) at 3 h. When glucose and exendin-(9-39) were infused through the portal and femoral veins, respectively, glucose clearance increased to 70.0 +/- 4.6 ml/kg.  min and glycemia decreased to 3.1 +/- 0.1 mmol/l, indicating that exendin-(9-39) has an effect only when infused into the portal vein.  Finally, portal vein infusion of glucose in GLP-1 receptor(-/-) mice failed to increase the glucose clearance rate (26.7 +/- 2.9 ml/kg.  min).  Glycemia increased to 8.5 +/- 0.5 mmol/l at 60 min and remained elevated until the end of the glucose infusion (8.2 +/- 0.4 mmol/l).  Together, our data show that the GLP-1 receptor is part of the hepatoportal glucose sensor and that basal fasting levels of GLP-1 sufficiently activate the receptor to confer maximum glucose competence to the sensor.  These data demonstrate an important extrapancreatic effect of GLP-1 in the control of glucose homeostasis.