The fluorescence study of the inclusion coordinated compound of beta-cyclodextrin and p-hydroxyphenolacetamide and its analytical applications. 
The inclusion coordinated compound fluorescence of p-hydroxyphenolacetamide (PHPA) with beta-cyclodextrin was studied and a high sensitive analytical method to determine the content of PHPA was established based on the increased fluorescence intensity of the coordinated compounds in Britton-Robinson buffer solution (pH 7).  The wavelength of the excited emission are 282 and 310 nm, respectively.  The enhanced coordinated compounds fluorescence intensity is proportional to the concentration of PHPA in the range 0.030-11.0 mg/l. The relative standard deviation (R.S.D.) were within 0.66-4.2%.  Correlation coefficient and inclusion binding constant Kf are obtained within 0.9950-0.9996 and 4.67 x 10(2), respectively.  Meanwhile, a linear increase of the resonance light scattering (RLS) with the inclusion coordinated compounds concentration was noticed at a synchronous wavelength of 584 nm.  The forming reasons of inclusion coordinated compounds were investigated.  The 1:1 molar ratio composition of inclusion compound was measured with DTA and elemental analysis.  The method is simple, rapid and has a good reproducibility.  The method was used for determination of PHPA in an artificial sample with satisfactory results.