[Human trabecular cell culture in vitro]. 
To search the method of human trabecular cell (HTC) cultured in vitro, which could provide the necessary condition of experimental study on primary open angle glaucoma.  Organ and tissue culture were taken to get the different passages of human trabecular cells.  In the meantime, human scleral fibrous cells were also cultured to contrast with HTC in morphology. ( 1) It took rather shorter time to get HTC by using organ culture method than simple tissue culture. ( 2) The 3rd to 5th passage of HTC was the most stable growth period, at that time they could be used in experiment.  Stable growth shape and characteristic HTC could be obtained by HTC culture in vitro.