Presence of gynogenetic males suggests a female heterogamety in sterlet Acipenser ruthenus L.
Investigation of the heterogametic sex in sterlet Acipenser ruthenus L. was performed using meiotic gynogenesis and gonadal histology.  Eggs from the albino females were activated by UV irradiated sperm of wild colored males and exposed to a heat shock.  The resultant fish were all albino and exhibited exclusively maternal inheritance of the microsatellite DNA markers.  Cytogenetic analysis indicated that gynogenetic progeny were diploids with 120 chromosomes.  Based on the histological analysis, more than 86% of the gynogenetic individuals were found to be females.  Moreover, some males (7%), sterile speciemens (3.5%) and fish with unidentified gonads (3.5%) were observed among the gynogenetic fish.  Presence of both females and males in the gynogenetic offspring is indicative that the heterogametic sex in sterlets is female.