H1 histone subtypes and subtype synthesis switches of normal and delobed embryos of Ilyanassa obsoleta. 
Histone H1 subtype complexity and H1 histone subtype synthesis switches were characterized during the development of normal embryos of the mud snail Ilyanassa obsoleta.  The effect of the removal of the third polar lobe on the normal H1 pattern of synthesis was then investigated in the delobed embryo to determine if classical polar lobe effects are accompanied by a perturbation of these patterns.  SDS-gel electrophoresis and fluorography of radiolabeled 5% perchloric acid-soluble nuclear extracts resolved six H1 proteins designated bands 1-6.  Bands 1-5 migrate as a cluster of individual bands with similar mobilities.  Band 6 has a substantially slower mobility.  The synthesis of band 6 is predominant during the first 6 hr post-trefoil.  During cleavage and gastrulation bands 1 and 2 are predominant while band 3, 4, and 5 become predominant during organogenesis.  In addition, it has been found that removal of the polar lobe delays the off-switch of the early bands 6, 1, and 2 and the on-switch of the late bands 3, 4, and 5.  This must result in a different H1 composition in the chromatin of the two embryo types.  Cell number data of normal and delobed embryos reveal that the delay in subtype synthesis switching is not caused by an overall delay of cell division in the delobed embryo.  However, the data indicate that a subpopulation of cells may not divide, or may divide late, in the delayed embryo.  The data also suggest that the D cell lineage may be involved in the control of histone synthesis switching in the A, B, and C cell lineages.