I have clone the gene in pUC19 and get conformation with restriction digestion.  But unfortunately, I get very low quantity of plasmid after miniprep.  While the control (circular pUC19) concentration is quite enough with miniprep kit that I used.  I shall be very glad to know about the possible reason of low concentration and how can I improve the yield of clone plasmid from miniprep. 
Yasir