ANNOUNCEMENT {#s1}
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Achromobacter insolitus strain Kg 19 is a bacterium isolated from soil samples taken from fields in the Krasnodar region of Russia (45°03′10.8″N, 38°52′22.8″E) that were subject to consequent glyphosate (GP) treatments over prolonged periods.  Isolation of the strain was carried out as follows: soil samples were incubated for 3 months at 28°C with periodic stirring and moistening with MS1 mineral medium ([@B1]) containing GP (18 mg kg^−1^ soil) as a component of the herbicide Roundup, inoculated into liquid MS1 medium with 10 g liter^−1^ glutamate and 0.5 g liter^−1^ GP, and incubated on a shaker with sequential passages twice per week (20 passages in total).  The resulting microbial associations were inoculated onto LB agar plates; the cultures were isolated according to the morphological distinctiveness of the colonies and cell size, shape, and mobility. 

*A. insolitus* Kg 19 cells, as a pure culture, were found to be Gram-negative mobile short rods approximately 1.4 to 2.5 × 0.6 μm with an optimal temperature of 28 to 30°C forming cream-colored glossy colonies.  The strain was routinely maintained on agar plates with MS1 mineral medium supplemented with 10 g liter^−1^ glutamate and 0.5 g liter^−1^ GP as the sole sources of carbon and phosphorus, respectively. * A. insolitus* Kg 19 obviously possessed enzyme systems capable of breaking the carbon-phosphorus (C--P) bond in a range of organophosphonates (OPn), as its growth was observed while cultivated under the same conditions but with 0.3 g liter^−1^ methylphosphonic acid, 0.35 g liter^−1^ 2-aminoethylphosphonate (2-AEP), or 0.33 g liter^−1^ aminomethylphosphonate (AMP) (all from Sigma-Aldrich, USA) as the sole phosphorus source. 

For genome sequencing, DNA isolation was performed using a FastDNA Spin kit and FastPrep-24 homogenizer (MP Biomedicals, USA).  The short-read library was prepared with a HyperPlus kit (Kapa, USA) in accordance with the manufacturer's recommendations.  In short, preparation included the following steps: enzymatic DNA fragmentation, end repair and A tailing, ligation of specific adapters, and amplification of the obtained library (7 cycles).  The library was sequenced on a NovaSeq 6000 platform (Illumina, USA) with a NovaSeq 6000 S2 reagent kit (200 cycles). 

Default parameters were used for all software unless otherwise noted.  The quality of the reads was checked with FastQC v0.11.8 ([@B2]).  Adapter sequences and low-quality regions in raw reads were cut with Trimmomatic v0.39 ([@B3]) using the following options: ILLUMINACLIP:TruSeq3-PE-2.fa:2:30:10; SLIDINGWINDOW:4:15; and MINLEN:30.  After trimming, the clean reads were assembled using SPAdes v3.14.0 ([@B4]) with the following options: \--cov-cutoff, auto; and \--careful.  The assembly quality was assessed with QUAST v5.0.2 ([@B5]).  Genome annotation was performed using the NCBI Prokaryotic Genome Annotation Pipeline ([@B6]). 

A total of 4.3 × 10^7^ paired-end reads with an average length of 101 bp (4.4 Gb) were obtained from the sequencing.  As a result, 4.2 × 10^7^ clean reads with an average length of 99 bp (4.1 Gb) were assembled into 43 scaffolds with 630-fold coverage.  The scaffold *N*~50~ value is 628,760 bp, and the largest scaffold is 973,458 bp.  The genome size is 6.4 Mb, with an average G+C content of 65.2%.  A total of 5,917 protein-coding genes (481 of which encode hypothetical proteins), 55 tRNAs, 6 complete or partial rRNAs, and 4 noncoding RNAs (ncRNAs) were predicted. 

The gene *PhnJ*, which encodes alpha-[d]{.smallcaps}-ribose 1-methylphosphonate 5-phosphate C--P lyase, a pivotal protein of OPn-degrading enzyme complex ([@B7]), was discovered in *A. insolitus* Kg 19 as a part of the complete C--P lyase operon.  The presence of the gene *phnX* encoding phosphonatase (phosphonoacetaldehyde hydrolase), the terminal hydrolase of the 2-AEP utilization pathway, was demonstrated as well.  This corresponds with the range of utilized OPn substrates and makes *A. insolitu*s Kg 19 a promising object of studies of OPn metabolism in bacteria and a possible candidate for the development of novel methods of bioremediation of soil and water polluted by GP or AMP. 

Data availability. {# s1.1}
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The raw reads have been deposited in the NCBI SRA under the accession no. [ SRR11236102](https://www.ncbi.nlm.nih.gov/sra/SRR11236102), and the whole-genome shotgun project has been deposited in DDBJ/ENA/GenBank under the accession no. [ JAALEO000000000](https://www.ncbi.nlm.nih.gov/nuccore/JAALEO000000000).  The version described in this paper is the first version, JAALEO010000000. 

This work was funded by the Russian Science Foundation (grant no.  18-74-00021). 

[^1]: **Citation** Tarlachkov SV, Epiktetov DO, Sviridov AV, Shushkova TV, Ermakova IT, Leontievsky AA.  2020.  Draft genome sequence of glyphosate-degrading *Achromobacter insolitus* strain Kg 19 (VKM B-3295), isolated from agricultural soil.  Microbiol Resour Announc 9:e00284-20.  <https://doi.org/10.1128/MRA.00284-20>.