Time-series analysis of the transcriptome and proteome of Escherichia coli upon glucose repression. 
Time-series transcript- and protein-profiles were measured upon initiation of carbon catabolite repression in Escherichia coli, in order to investigate the extent of post-transcriptional control in this prototypical response.  A glucose-limited chemostat culture was used as the CCR-free reference condition.  Stopping the pump and simultaneously adding a pulse of glucose, that saturated the cells for at least 1h, was used to initiate the glucose response.  Samples were collected and subjected to quantitative time-series analysis of both the transcriptome (using microarray analysis) and the proteome (through a combination of 15N-metabolic labeling and mass spectrometry).  Changes in the transcriptome and corresponding proteome were analyzed using statistical procedures designed specifically for time-series data.  By comparison of the two sets of data, a total of 96 genes were identified that are post-transcriptionally regulated.  This gene list provides candidates for future in-depth investigation of the molecular mechanisms involved in post-transcriptional regulation during carbon catabolite repression in E. coli, like the involvement of small RNAs.