Multiplexed labeling system for high-throughput cell sorting. 
Flow cytometry and fluorescence activated cell sorting techniques were designed to realize configurable classification and separation of target cells.  A number of cell phenotypes with different functionalities have recently been revealed.  Before simultaneous selective capture of cells, it is desirable to label different samples with the corresponding dyes in a multiplexing manner to allow for a single analysis.  However, few methods to obtain multiple fluorescent colors for various cell types have been developed.  Even when restricted laser sources are employed, a small number of color codes can be expressed simultaneously.  In this study, we demonstrate the ability to manifest DNA nanostructure-based multifluorescent colors formed by a complex of dyes.  Highly precise self-assembly of fluorescent dye-conjugated oligonucleotides gives anisotropic DNA nanostructures, Y- and tree-shaped DNA (Y-DNA and T-DNA, respectively), which may be used as platforms for fluorescent codes.  As a proof of concept, we have demonstrated seven different fluorescent codes with only two different fluorescent dyes using T-DNA.  This method provides maximum efficiency for current flow cytometry.  We are confident that this system will provide highly efficient multiplexed fluorescent detection for bioanalysis compared with one-to-one fluorescent correspondence for specific marker detection.