I-Ultrasonic Tissue Homogenizer - Hielscher Ultrasound TechnologyI-Ultrasonication iyindlela ephephile neyasebenza ye-homogenization yezicubu.Izicelo ze-ultrasonic zihlanganisa izicubu zokulungiselela kanye nezimo zokuphefumula, ukukhipha amaprotheni, i-DNA / i-RNA nama-compounds asebenzayo, noma ukuvuselelwa / ukungasebenzi kwe-enzyme nemvubelo.Ukulawula kahle kwe-ultrasound kuvumela ukulungiselela isampula okuhlosiwe.I-homogenization yama-tishu iyinqubo yokulungiselela isampula lapho amaseli wesilwane nesitshalo noma ama-microorganisms ekulungiswa ngaphambi kokukhishwa kwendaba ye-intracellular njengama-protein, i-DNA noma i-RNA. I-membran yeseli kufanele iphulwe ukuze ikhulule okuqukethwe kweli cell. Ngemuva kokuphula udonga lwamaseli, ama-macromolecules angenangqamuzana athamela kwisisombululo se-buffer ukuze i-organelles, amaprotheni ne-DNA / i-RNA itholakale. Ama-homogenizers we-Ultrasonic namasilensi weseli ngamathuluzi asebenza kahle kakhulu cell ukuphazanyiswa, lysis futhi Ukukhipha.Ukulungiselelwa okuningi kwesampula nge-sonication.I-homogenizers ye-Ultrasonic isetshenziswa ngokujwayelekile ukuze izicubu zigaya, ukuphazanyiswa kwamaseli & i-lysis kanye ne-homogenization yamasampuli wezinto eziphilayo ngaphambi kokukhishwa kwendaba ye-intracellular kuleso sikhala samangqamuzana. Nge-sonication elawulwayo yamasampuli, zonke izinyathelo ezivela ku-lysis kuya kokukhipha kanye nokwenziwa kwe-homogenization zingenziwa ngokusebenzisa i-ultrasonic cell disruptor efanayo.Inzuzo enkulu ye homogenizers ultrasonic ilele kalula amandla aguquguqukayo ukufaka nokuqina kwe-sonication. Ama-ultrasonic cell disruptors (i-lysers) avumela ukulawula amandla we-sonication ngokulungisa ukuphakama kusuka ku-20% ukuya ku-100%.Ngaphandle kokusebenza okuqhubekayo, i-homogenizer ye-ultrasonic ingasethwa ukujikeleza imodi, isb for sonication of izicubu ezibucayi. I-Sonication kumodi ye-pulse kusho i-ultrasonicator edala amagagasi aphezulu kakhulu e-ultrasonic kumjikelezo wezinkathi. Ubude besikhathi sokunikezwa kwesikhathi kanye nesikhathi esithile kungenziwa kubekwe ngabanye ngabasebenzisi.AT I-Sonication ene-Pulsed, phakathi nesikhathi sokuphumula, izinto eziphathekayo zingabuyela esimweni sayo sokuphumula futhi isampuli inganciphisa (usebenzisa isikhathi esithile sokushisa ukushisa). Ngaleyo ndlela, ukucubungula kahle kungasethwa ngokunembile ngokuhambisana nezicubu kanye nokushisa kwesampula okungadingeki kuncishisiwe.Ama-homogenizers ama-ultrasonic amancane futhi asebenzise umsebenzisi ngenkathi eveza imiphumela enokwethenjelwa eqinisekisayo ukukhiqizwa kabusha ngenxa yokulawula okugcwele kuzo zonke izinqubo ezibalulekile zenqubo.Izicelo ezivamile zezigaba ze-ultrasonic izicubu zokugaya, ukuphazanyiswa kwamangqamuzana, i-lysis, i-extraction kanye ne-homogenization zitholakala kuma-laboratories eziphilayo nezidakamizwa, i-microbiology, i-proteomic kanye ne-genomic ucwaningo (isib. Amaprotheni ukukhululwa ngaphambi kwe-Western blot kanye ne-enzyme-linked-immunosorbent assay), kodwa futhi ekukhiqizeni of ukukhishwa futhi amakhemikhali asebenzayo ngokuba izimboni zokudla, i-pharma nezimonyo.Thola idivayisi ephezulu ye-ultrasonic yezidingo zakho:Hielscher ultrasonic izicubu homogenizers ziyatholakala nge ultrasound okukhipha uhla phakathi 50W kuya 16,000W (16kW).Abasebenzi beLatroli bayathokozisa ngokusebenza okulula futhi okunembile kwama-homogenizers we-Hielscher's ultrasonic lab. Kuye ngezidingo ezithile, amadivaysi e-Hielscher afika ngokulawula kwe-digital noma analog.I I-VialTweeter iyisetshenziswa esinamandla se-ultrasonic evumela ukuthi i-sonication yesikhathi esisodwa ibe nezikhumba ezifika ku-10 noma ama-tubes wokuhlola ngendlela efanayo. Ngenxa yokungena okungaqondile, ukungcola okuphambene nokuphulukiswa kwesampuli kugwenywe ngokuphelele. Ukuhlanza nokuhlanza kuncishiswe kuncishiswe ngenxa yokusetshenziswa kwezigqoko ezilahlwayo. I-sonotrode ye-block ikwazi ukuzenzekela.Kububanzi be-probe-uhlobo lab ultrasonicators, amadivayisi angakhethwa 50W, 100W, 200W, 250W futhi 400W.Ukuze uthole izinqubo ezinkulu, izinsizakalo ze-bench-top ultrasonic ziyatholakala nazo 500W, 1000W, 1500W futhi 2000W.Futhi ngenxa yemifula ephezulu yamanzi, ama-ultrasonicators of 4000W, 10,000W noma 16,000W zihlanganiswa futhi ngaleyo ndlela zikwazi ukucubungula futhi zenze i-homogenize cishe yonke inani lezinhlamvu zeselula.Izinzuzo ezinkulu zama Ultrasonicators kaHielscher:ukugaya ngokushesha nokuphumelelayo, homogenization, lysis & ukuphazamiseka kweseli, noma ukukhishwa yi-cavitation enamandla e-ultrasonicukusetshenziswa okulula futhi enembile nokusebenza (khetha i-analog noma i-digital version)ukuhlanza okulula kokusebenza komkhiqizo ngokususa i-sonotrode ngaphansi kwe-sonication (i-CIP ehlanzekile-in-place / i-SIP inzalo-in-place)ibhokisi lokuvikelwa komsindo (ibhokisi lokuvikelwa komsindo lamadivaysi ebhubhu alenziwe ngokuphelele kusuka engilazini ye-acrylic ukuze kubonakale ngokugcwele kunoma iyiphi i-angle)ukulawula okugcwele phezu kwemigomo yenqubo nge-digital touch-display kudivayisi (i-digital ultrasonicators) noma i-powermeter (yamadivayisi we-analog)ukuhlanganiswa kokushisa kokushisa (okubalulekile ekushiseni okubucayi, amasampuli ama-labile)Ukumiswa kwe-homogenization run kungachazwa ngokucacile futhi kulungiselelwe ngaphambilini kumenyu ye-digitalwonke amadivaysi edijithali afakwe ukurekhoda kwedatha okuzenzakalelayo (ku-SD / USB-ComboCard edidiyelwe), inzwa yokushisa ye-plugable kanye nesilawuli esikude sesiphequluli.ukwelashwa okuningi kwesampula ne I-VialTweeter noma ngokusebenzisa i-sonotrodes engu-4- noma engu-8-umunwe (isb. ukuhlanganiswa kwezinqubo zebhobhobhothi yebholabhuthi)izinhlelo zokusebenza ezincane nezinkuluChofoza lapha ukuze uthole izincomo zenkambiso ethile mayelana nokulungiselela isampula, i-homogenization yezicubu, i-lysis kanye ne-extraction (isb. Amangqamuzana, amabhaktheriya, ama-spores, ama-compounds asebenzayo)!Ultrasonic Isizinda of yeselula MatterI-Ultrasonic Lysis ye-Western BlottingUkuqeda amandla kuyisicelo esivamile se-ultrasonication. Le vidiyo ikhombisa ukuqedwa okusheshayo kwamakhristalu kashukela emanzini kusetshenziswa iHielscher UP200St.Ukuqumba Amakristalu Ushukela Emanzini usebenzisa i-UP200StUltrasonic izicubu homogenizer UP200StI-ultrasonicators UP200Ht ne-UP200St zombili izinhlobo ezinamandla zama-hW200gen homogenizer zesampula se-prep, i-emulsation, ukusabalalisa, isizinda kanye ne-chemistry.I-UP200Ht - I-Handheld Ultrasonic HomogenizerIsicelo sokuphindaUP200Ht – I-digital homogenizer ye-digital ultrasonic labI-UIP1000 – I-Sonication Setha1,000 watts isethi ultrasonic ngenqubo R&D nokusebenza kahle
