Ukukhiqizwa kwe-Ultrasonic-Enzymatic Diacylglycerol - Hielscher Ultrasound TechnologyI-Diacylglycerol (DAG) amafutha acebile abonise inani eliphakeme lokudla okunomsoco njengoba ligajwa futhi lihlelwe ngendlela ehambisanayo, okuyinto eyanciphisa kakhulu isisindo somzimba.I-Diacylglycerol ingakhiqizwa yi-hydrolysis yamafutha esundu ngokusebenzisa i-lipase yezohwebo njenge-catalyst ngaphansi kwe-ultrasonication.Nge-hydrosonic-enzymatic hydrolysis, ama-DAG angakhiqizwa eminyangweni ephezulu ngezindleko zokucubungula kakhulu.Nge-hydrolysis eyasiza kakhulu i-bio-catalyzed, amafutha yemifino ejwayelekile angashintshwa abe amafutha a-DAG acebile anenani eliphezulu lokudla okunomsoco. I-ultrasonic-enzymatic hydrolysis inikeza isivuno esihle se-diacylglycerol-ocebile oyelayo ngezikhathi ezimfushane zokuphendula futhi ngaphansi kwezimo ezibucayi.Izinzuzo ze-ultrasonic hydrolysis:i-emulsification enhleizimo ezinomusaisikhathi esifushane senquboukushisa okulawulwayoukukhiqizwa ngaphakathiUcwaningo & ImiphumelaAwadallak et al. (2013) baye baphenya nge-ultrasonically basiza i-hydrolysis yamafutha esundu ngama-Lipozyme RM IM njenge-biocatalyst. Esikhathini esithatha izinyathelo ezimbili, i-ultrasound isetshenziselwa ukukhuthaza i-emulsification yamafutha namanzi. Esinyathelweni sesibili, ama-enzyme ayengezwa ukuguqulwa kokucubungula.Isethaphu ye-ultrasonic njengoba isetshenziselwa ucwaningo lwe-Awadallak: UP200S (200W, 24kHz) nge-glass flow cell (bheka isithombe ngakwesobunxele).Iqembu lokucwaninga lithole ukuthi ukulandela imiphumela yezinyathelo ezimbili kulemiphumela emihle kakhulu: i-probe ye-ultrasound yayifakwe ekujuleni okungaba ngu-10mm emanzini wamanzi / amafutha, amandla ahlelwe ku-80 W futhi avuliwe amaminithi amathathu ukuze aphumelele isistimu ngaphambi kokuba isuswe, bese i-enzyme (1.36 wt.% yamanzi + mass) yanezelwa ngenkathi isixazululo sasihlanganiswa nokuvutha kwamagnetic (300 rpm).Ukwelashwa kwe-ultrasound kwenziwa nge-the i-ultrasonic probe idivayisi ye-UP200S.Ngakho-ke ama-bio-catalysis asetshenzisiwe nge-ultrasonically akhiqiza amafutha e-DAG nge-34.17 wt.% Ukuhlushwa ngemva kwesikhathi esingu-12h sokuphendula. Isinyathelo sonication ngokwayo sasincane kakhulu isikhathi esingamaminithi angu-1.2 kuphela.Njengoba i-sonication isetshenziswa njengesinyathelo sokuqala ukuthola imulsion ye-ultrasonic emihle, le nqubo yezinyathelo ezimbili inenzuzo yokukhiqizwa okukhulu: Izindleko zayo zamandla ziphansi kakhulu futhi isikhathi sayo esifushane sokuthi i-emulsification ivumela ukusetshenziswa kwemishini eqhubekayo ye-ultrasonic yokudla ezinkulu ze-hydrolysis reactors.I-ultrasonic emulsion encaneIzinsiza ze-Ultrasonic ze-Pilot & ukukhiqizwaI-Hielscher Ultrasonics ingumlingani wakho wesikhathi eside ohlangenwe nakho wezinqubo ze-ultrasonic. Sakha izinhlelo ezinamandla nezinokwethenjelwa ze-ultrasonic nganoma yiliphi ivolumu. Njengomhlinzeki embonini yezokwelapha kanye nokudla, sisebenza ngokukhethekile ekukhiqizeni imishini ye-ultrasonic kanye nokubonisana ngezinqubo ze-ultrasonic kanye nokuqaliswa kwazo emithonjeni yokukhiqiza.Ithebula elingezansi linikeza isibonakaliso somthamo wokucubungula we-ultrasonicators wethu.I-MultiPhaseCavitator ye-HielscherI-Hielscher ithuthukise ukufaka okuyingqayizivele kwesitfombe se-cell cell MultiPhaseCavitator MPC48. Ukufaka i-MPC48 (bheka isithombe ngakwesokudla) ifakwe ama-cannula ama-48 amnandi, lapho isigaba sesibili sijova khona njengomsakazo omuhle kakhulu we-liquid (diameter of 0.3mm kuya ku-1.2mm) kuye ngokuthi ubukhulu be-cannula). Njengoba isigaba sesibili sijojowe kakade kahle kakhulu endaweni ye-ultrasonic cavitation, i-micro- noma nano-emulsion ikhiqizwa ngu-sonication. Inhlanganisela ye-MPC48 yenzelwe ama-reactors esebenzayo we-Hielscher futhi ingasetshenziselwa i-batch nokuqhutshwa okuqhubekayo.I-Ultrasonic Flow Cell Reactor FC100L1K-1S nge-InsertMPC48Faka i-InsertMPC48 ngemiphumela engcono kakhulu ye-emulsionI-Awadallak, i-Jamal A .; U-Voll, uFernando; I-Ribas, uMarielen C .; da silva, camila da; Filho, Lucio Cardozo; da Silva, u-Edson A. (2013): i-enzymatic catalyzed oil hydrolysis ngaphansi kwe-ultrasound irradiation: i-Diacylglycerol synthesis. Ultrasonics Sonochemistry 20; 2013. 1002-1007.I-Dhara R .; Dhar P .; I-Ghosh M. (2013): Imiphumela yokudla ka-diacylglycerol ocebile oyeladi lwesinaphi kwi-lipid profile yama-rats normocholesterolemic nama-hypercholesterolemic. Journal of Food Science Technology 50 (4); 2013. 678-86.I-Dhara R .; Dhar P .; I-Ghosh M. (2012): Imiphumela yokudla ye-oli ehlanzekile ne-diacylglycerol ene-rice bran oil ekwenzeni ukukhula kanye nephid profile yezinja. Journal of Oleo Science 61 (7); 2012. 369-75.UGoncalves, uKaren M .; Sutili, Felipe K .; I-Leite, i-Selma GF; de Souza, u-Rodrigo OMA; URamos Leal, i-Ivana Correa (2012): i-hydrolysis yamafutha e-palm ekhishwa yi-lipases ngaphansi kwe-ultrasound irradiation - Ukusetshenziswa komklamo wokuhlola njengethuluzi lokuhlola okuguquguqukayo. Ultrasonics Sonochemistry 19; 2012: 232-236.U-Souza, u-Rodrigo OMA; I-Babicz, i-Ivelize; I-Leite, i-Selma GF; I-Antunes, i-Octavio AC: i-Lipase-Catalyzed Diacylglycerol Production ngaphansi kwe-Sonochemical Irradiation.I-Ultrasonic-Enzymatic Oil HydrolysisI-Ultrasonic Margarine ProductionMayelana ne-DiacylglycerolsI-Diacylglycerols (DAG) isetshenziswe ngokujwayelekile emazingeni ahlanzekile ahlukahlukene njengezithasiselo zokuthuthukisa i-plasticity yamafutha noma izisekelo zokudla, imithi nezimboni zezimonyo. I-DAG nayo isetshenziselwa amafutha e-estranger ukuhlukanisa izinto ezivela ezibunjiweni kanye nokuguqula amafutha, ama-precursor for synthesis organic products ezifana ne-phospholipids, i-glycolipids, i-lipoproteins, izidakamizwa ezinjenge-DAG-i-chlorambucil ephathekayo yokwelapha i-lymphoma, (S ) - (3.4-dihydroxyphenyl) alanine (i-LDOPA) yokwelashwa kwesifo sikaParkinson nabanye abaningi. Muva nje, amafutha a-DAG asele asetshenzisiwe njengamafutha okupheka asebenzayo, anokuqukethwe okungenani okungu-80% we-1,3-DAG.I-Diacylglycerol (DAGs) ingakhiwa yi-hydrolysis, ingxenye ye-esterification noma i-glycerolysis ngokusebenzisa i-chemical or enzymatic catalysis. I-Sonication iye yafakazelwa ukuqinisa i-catalytic enzymatic ye-diacylglycerol kakhulu. I-ultrasonic-enzymatic hydrolysis ivumela isivuno esiphezulu sezinga eliphezulu ze-DAGs ngesikhathi esifushane kakhulu senqubo.
