I-Ellagitannin Khipha ku-Pomegranate - Hielscher Ultrasound TechnologyI-Ellagitannins iyisandulela se-urolithin A, eyaguqulwa kusukela e-ellagitannins yi-microbiome yegciwane.Isizinda se-ultrasonic sithuthukisa ukukhululwa kwe-ellagitannins kusuka kumangqamuzana wezithelo nezitshalo ukukhiqiza izithako kanye nezidakamizwa ngokuqukethwe okugxilile kwe-ellagitannin.I-Ellagitannins – I-Precursor of Urolithin AI-Urolithin A isanda kuthola ukunakwa okuningi njengoba ukuhlolwa kwabantu kugcizelela imiphumela emihle empilweni nasekuphiliseni isikhathi eside. I-Urolithini A ikhiqizwa yi-microflora yegundane ngemuva kokugcoba izidakamizwa ezitholakala kwikomegranate njenge-ellagitannins kanye ne-punicalagins. I-Urolithini A ivuselela imitophagy futhi iyasiza ngaleyo ndlela ukuvuselela i-mitochondria enesici. Njengoba impilo ye-mitochondrial neyemaselula iyancipha uma ikhula, ukuphathwa kwe-ellagitannin kungaba yindlela yokwelapha entsha yokwelashwa ngokuphathelene nezinkinga ezihlobene nobudala. Imiphumela ezuzisayo yayigcinwe kuCelegans, amangqamuzana ama-mammalian, ama-rodents, futhi ihlolwe ngempumelelo emitholampilo ekuvivinyweni kwabantu. Ngaphandle kwemiphumela yayo yokudala iminyaka, ama-ellagitannin ayimithi ebaluleke kakhulu empilweni ngenxa yemiphumela yabo yokulwa nokuvuvukala nokulwa nomdlavuza.Ukukhishwa kwe-ultrasonic kwe-EllagitanninsIsizinda esisizwa nge-ultrasound (UAE) sezinto zokudla sezitholile isithakazelo esikhulu, ngoba kuyabiza kakhulu. Ihlula ukungalungi kwezingqikithi zendabuko ye-solvent futhi, ngesikhathi esisodwa, inikeza ubuningi obukhulu bezingqikithi ngesikhathi esisodwa, usebenzisa amanani amancane okuxazululwa kwezinga lokudla noma amanzi nje. Ama-ultrasound ayakwazi ukuphula amazindonga eseli ukukhipha uketshezi lwe-intracellular. Amandla aphezulu, amagagasi e-ultrasound aphindaphindiwe enza ama-molecule of medium medium, okungukuthi i-solvent, ngokushintsha izikhathi zokucindezela kanye nezikhathi zokungaqabuki kwamakhemikhali. Lezi zindwangu zokucindezela nokwandisa imijikelezo kubangela ukwakheka kwe-voids noma ama-bubbles emoyeni okukhula kancane kancane kuze kufike implosion, okwenza kube khona umphumela we-cavitation. I-UAE ingasetshenziswa kokubili ngezinga elincane nelincane embonini yokukhipha ukudla.Amapomegranati acebile ku-ellagitannins.Isizinda se-ultrasonic sikhipha isisindo esiphelele sezakhi ezisuka kumangqamuzana wemifino. Ukukhishwa kwamapomegranate, indodanaication kusiza ukuhlukanisa i-ellagitannins, i-ellagic acids, i-pomegranatate, i-punicanolic acid (uhlobo lwe-triterpenes olutholakala emafutheni abalulekile) nezinye izinto eziningi zokuvimbela i-antioxidants, amavithamini nama-polyphenols. Izitshalo ezigcwele ze-spectrum ziyaziwa ngemiphumela yazo ephakeme ngenxa yethonya elibizwa nge-entourage effect. Umthelela we-entourage uchazwa yi-interplay synergistic yezakhi ezihlukahlukene ezitshalweni, okuthuthukisa ukutholakala kwezinto eziphilayo kanye nemiphumela emihle kakhulu yezempilo.Ama-Ultrasonic Processors for ExtractionHielscher Ultrasonics ngokukhethekile ekukhiqizeni high-ukusebenza ultrasonic isizinda imishini for batch nokuqhubeka flow flow izinqubo. Amaprosesa ase-Ultrasonic avela ebhentshini-phezulu kanye nomshayeli wezindiza kuya esikalini sezimboni atholakala kalula futhi angenziwa kalula ngezidingo ezithile zenqubo yakho.ukuphakama okukhulu kwe-ultrasound ukuze kusetshenziswe i-extraction kanye nokuhlukaniswa kwezingxenye eziphilayo (isb quercetin, i-caffeine, i-curcumin, Terpenes njll). Wonke amadivayisi we-ultrasonic avela 200W kuya 16,000W kufaka isibonisi ezinemibala yokulawula idijithali, ikhadi elihlanganisiwe le-SD lokuqopha okuzenzakalelayo kwedatha, ukulawula okukude kwesiphequluli kanye nezinye izici eziningi zomsebenzisi. I-sonotrodes namaseli okugeleza (izingxenye, ezithintana nephakathi) zingenziwa nge-autoclaved futhi kulula ukuzihlanza. Wonke ama-ultrasonicators ethu akhiwe ngomsebenzi we-24/7, adinga ukugcinwa okuphansi futhi kulula futhi ephephile ukusebenza.Amakhasimende ethu ayazisa ukuqina nokuthembeka kwezinhlelo zethu ze-ultrasonic. Ukurekhoda kwedatha okuzenzakalelayo kwekhadi le-SD ekhiqizwa kuvumela ukulawula okucacile kwenqubo, ukuphindaphinda kanye nomshuwalense wezinga eliphezulu lomkhiqizo oqhubekayo ngokuhambisana neMikhuba Emihle Yokukhiqiza.Andreux, PA; UBlanco-Bose, W .; Ryu, D .; I-Burdet, F .; Ibberson, M .; I-Aebischer, i-P .; I-Auwerx, i-J .; Singh, A .; I-Rinsch, C. (2019): I-activator ye-mitophagy urolithin A iphephile futhi inomthelela isignesha yamangqamuzana ekuthuthukiseni impilo ye-mitochondrial kanye namaselula kubantu. Uhlobo lweMetabolism 1, 2019. 595-603.I-Boggia, R .; I-Turrini, F .; I-Villa, i-C .; I-Lacapra, iC .; I-Zunin, i-P .; I-Parodi, B. (2016): Ukukhipha Okuhlaza Okuvela Kwempomegranate Marcs Ukukhiqiza Ukudla Okusebenzayo Nezimonyo. Imithi 9/63, 2016.I-Wu S.; I-Tian L. (2017): Amakhemikhali Amakhemikhali Ahlukahlukene kanye Nezinto Eziphilayo Emthonjeni Wasendulo Namafutha Asemsebenzini Asemapulagranati (iPunica granatum). Ama-molecule 22 (10), 2017. 1606.I-EllagitanninsI-Ellagitannin yiqembu elihlukene lama-tannin ahanjiswa ngamanzi, uhlobo lwe-polyphenol eyakhiwe ngokuyinhloko ekuxhumaneni okuxhumene kwamaqembu ase-galloyl ku-1,2,3,4,6-pentagalloyl glucose. I-Ellagitannins ne-ellagic acid kukhona i-polyphenols ekhona kwezinye izithelo, amantongomane nembewu, njengamahalananda, ama-raspberries amnyama, ama-blackberries, ama-raspberries, ama-strawberries, ama-walnuts nama-alimondi. Ama-alglakhi acid ahlobene nama-polyphenols, atholakala kuma-walnuts, ama-pecans, ama-cranberries, ama-raspberries, ama-strawberries, amagilebhisi, amapreaches kanye ne-pomegrante kanye nezinhlobo ze-oki ezifana ne-North American white oak (i-Quercus alba), i-European oak obomvu (i-Quercus robur) ku-mushroom okwelapha Phellinus linteus.Ama-orolithini angama-metabolite emathumbu emathumbu avela emanzini ase-ellagitannins. I-Urolithin A yathandeka kakhulu nje ukukhathazeka okuningi kusukela abacwaningi babonise izinzuzo eziyingqayizivele ze-urolithin A ngempilo ye-mitochondrial.Imisebenzi ephikisana ne-antiproliferative kanye ne-apoptosis ye-ellagic acid nama-urolithini aboniswe ukuvinjelwa komdlavuza we-cell cell.I-Ellagitanins nomthombo wawo: I-Castalagin (i-oki, izinkuni ze-chestnut), i-Castalin (izinkuni ze-oki, amaqabunga e-punk), i-Casuarictin (i-Casuarina ne-Stachyurus), i-Crandinin (amaqabunga e-punk tree, i-Peduncalagin (i-walnuts), i-Punicalagin (igrunate), i-Punicalin ( igromegranate), uRoburin A (izinkuni ze-oki), i-Tellimagrandin II (i-clove, i-Asian herb bennet), i-Terflavin B (i-black chebulic, i-almonds yamaNdiya), i-Vescalagin (i-oki, i-chestnut wood).Ama-metabolite ayenziwe ngamapomegranate ellagitannin. (A) I-punicalagin (i-pomegranate ellagitannin) i-hydrolyzed i-hexahydroxydiphenic acid, ekhishwa ngamanzi ukuze ihambise i-ellagic acid; (B) Izakhiwo zamakhemikhali ezinama-orolithini abamele, imikhiqizo yokuguqulwa kwamagciwane ezincane ze-ellagic acid. [Wu; I-Tian: 2017]I-MitochondriaI-mitochondrion (i-mitochondria eningi) iyinhlangano engaphansi kwe-membrane-bounded etholakala ezinhlobonhlobo eziningi ze-eukaryotic. I-mitochondria yilapho okuthiwa izitshalo zamandla emangqamuzaneni futhi igcwalise inqwaba yemisebenzi ebalulekile ngokudala ingxenye eyinhloko yesondlo sika-adenosine triphosphate (ATP), esetshenziselwa njengamandla wamakhemikhali. Ukuphefumula kwe-mitochondrial kuyisethi yokuphendula komzimba kanye nezinqubo ezidinga oksijeni okwenzeka mitochondria ukuguqula amandla agcinwe kuma-macronutrients ku-adenosine triphosphate (ATP), i-energy donor yonke emangqamuzaneni. I-macromolecules ye-mitochondrion, kuhlanganise ne-chain of respiratory chaines, iyakwazi ukulimala okwenziwe nge-oxidative ehambisana nokuvuvukala. Ama-ellagitannins nama-urolithini avela emapomegrante awabonwanga ukuthi asebenzise ama-mitophagy (apoptosis) ukuze asuse i-mitochondria eyonakalisiwe ukuze i-mitochondria eyonakaliswe ingene esikhundleni se-organelles enempilo.
