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Migration rate dependency
Electrophoresis
Directly proportional to rate of movement
Charge of the molecule
Migration rate dependency
Electrophoresis
Inversely proportional to rate of movement
Size of the molecule
Migration rate dependency
Electrophoresis
Increased current increases migration rate
Electrical field
Migration rate dependency
Electrophoresis
Increased ionic strength decreases migration rate
Ionic strength of buffer
Migration rate dependency
Electrophoresis
Decreased pH slows migration
pH of buffer
Migration rate dependency
Electrophoresis
Inversely proportional to migration rate
Viscosity of supporting medium
Migration rate dependency
Electrophoresis
High temperature can denature protein and slow migration
System temperature
Analytic electrophoretic procedures
Electrophoresis
Proteins are amphoteric...
Protein electrophoresis
Analytic electrophoretic procedures
Electrophoresis
Similar to protein electrophoresis...
Isoenzyme electrophoresis
Components
Immunoassay
A substance that can elicit an immune response...
Antigen
Components
Immunoassay
An immunoglobulin formed in response to a foreign substance...
Antibody
Immunochemical labels
Immunoassay
Attached to the antibody, allowing colorimetric quantitation...
Enzyme labels
Immunochemical labels
Immunoassay
Emit photons when excited, used in fluorescence-based detection...
Fluorescent labels
Immunochemical labels
Immunoassay
Release energy in the form of visible light upon returning to ground state...
Chemiluminescent labels
Immunochemical labels
Immunoassay
Emit radiation (gamma rays) upon decay, detected for quantitation...
Radioisotope labels
Methodologies
Immunoassay
Enzyme-based
ELISA
Methodologies
Immunoassay
Enzyme-based
EIA/EMIT
Methodologies
Immunoassay
Fluorescence-based
FPIA
Methodologies
Immunoassay
Fluorescence-based
FIA
Methodologies
Immunoassay
Radiation-based
RIA
Methodologies
Immunoassay
Radiation-based
IRMA
Mechanisms of separation
Chromatography
Based on the competition between the sample and the mobile phase for binding sites...
Adsorption chromatography
Mechanisms of separation
Chromatography
Depends on the solubility of the solute in nonpolar or polar solvents...
Partition chromatography
Mechanisms of separation
Chromatography
Involves separation of solutes by their size and charge of the ionic species present...
Ion-exchange chromatography
Chromatographic procedures
Chromatography
Semi-quantitative screening test...
Thin-layer chromatography (TLC)
Chromatographic procedures
Chromatography
Provides quantitative results...
High-performance liquid chromatography (HPLC)
Migration rate dependency
Electrophoresis
Directly proportional to rate of movement
Charge of the molecule
Migration rate dependency
Electrophoresis
Inversely proportional to rate of movement
Size of the molecule
Migration rate dependency
Electrophoresis
Increased current increases migration rate
Electrical field
Migration rate dependency
Electrophoresis
Increased ionic strength decreases migration rate
Ionic strength of buffer
Migration rate dependency
Electrophoresis
Decreased pH slows migration
pH of buffer
Migration rate dependency
Electrophoresis
Inversely proportional to migration rate
Viscosity of supporting medium
Migration rate dependency
Electrophoresis
High temperature can denature protein and slow migration
System temperature
Analytic electrophoretic procedures
Electrophoresis
Proteins are amphoteric...
Protein electrophoresis
Analytic electrophoretic procedures
Electrophoresis
Similar to protein electrophoresis...
Isoenzyme electrophoresis
Components
Immunoassay
A substance that can elicit an immune response...
Antigen
Components
Immunoassay
An immunoglobulin formed in response to a foreign substance...
Antibody
Immunochemical labels
Immunoassay
Attached to the antibody, allowing colorimetric quantitation...
Enzyme labels
Immunochemical labels
Immunoassay
Emit photons when excited, used in fluorescence-based detection...
Fluorescent labels
Immunochemical labels
Immunoassay
Release energy in the form of visible light upon returning to ground state...
Chemiluminescent labels
Immunochemical labels
Immunoassay
Emit radiation (gamma rays) upon decay, detected for quantitation...
Radioisotope labels
Methodologies
Immunoassay
Enzyme-based
ELISA
Methodologies
Immunoassay
Enzyme-based
EIA/EMIT
Methodologies
Immunoassay
Fluorescence-based
FPIA
Methodologies
Immunoassay
Fluorescence-based
FIA
Methodologies
Immunoassay
Radiation-based
RIA
Methodologies
Immunoassay
Radiation-based
IRMA
Mechanisms of separation
Chromatography
Based on the competition between the sample and the mobile phase for binding sites...
Adsorption chromatography
Mechanisms of separation
Chromatography
Depends on the solubility of the solute in nonpolar or polar solvents...
Partition chromatography
Mechanisms of separation
Chromatography
Involves separation of solutes by their size and charge of the ionic species present...
Ion-exchange chromatography
Chromatographic procedures
Chromatography
Semi-quantitative screening test...
Thin-layer chromatography (TLC)
Chromatographic procedures
Chromatography
Provides quantitative results...
High-performance liquid chromatography (HPLC)