""" ---------------------------------------- * Creation Time : Tue Jun 13 14:18:20 2023 * Author : Charles N. Christensen * Github : github.com/charlesnchr ----------------------------------------""" import streamlit as st import matplotlib.pyplot as plt import matplotlib.patches as patches import numpy as np from skimage import io import tifffile as tiff import os # Function to draw a rectangle given four points def draw_rectangle(points, ax): # Compute the centroid of the points centroid = np.mean(points, axis=0) # Compute the width and height width = np.linalg.norm(points[1] - points[0]) height = np.linalg.norm(points[2] - points[1]) # Compute the angle angle = np.arctan2(points[1, 1] - points[0, 1], points[1, 0] - points[0, 0]) # Create the rectangle rect = patches.Rectangle( centroid, width, height, angle=np.degrees(angle), linewidth=1, edgecolor="gray", facecolor="none", transform=ax.transData, ) # Add the rectangle ax.add_patch(rect) ax.set_aspect("equal") def draw_rectangle_from_coords(ax, ridx, cidx): l = np.sqrt(2) / 2 # unit length o = 8 # origin (x,y) shift = 1 if ridx % 2 == 0 else 0 x1 = o - 2 * l * cidx + shift * l y1 = o - l * ridx x2 = o - l - 2 * l * cidx + shift * l y2 = o + l - l * ridx x3 = o - 2 * l * cidx + shift * l y3 = o + 2 * l - l * ridx x4 = o + l - 2 * l * cidx + shift * l y4 = o + l - l * ridx # apply shift based on row and column # Parse points points = np.array([x1, y1, x2, y2, x3, y3, x4, y4]).reshape(-1, 2) draw_rectangle(points, ax) # annotate ridx and cidx in the center of the rectangle ax.annotate( f"{ridx},{cidx}", xy=(x2, (y1 + y3) / 2), horizontalalignment="center", verticalalignment="center", color="orange", ) # return min max x and y return ( min(x1, x2, x3, x4), max(x1, x2, x3, x4), min(y1, y2, y3, y4), max(y1, y2, y3, y4), ) def plot_patch(patch): fig, ax = plt.subplots() bounds_list = [] # iterate through patch and render if pixel is 1 for row in range(patch.shape[0]): for col in range(patch.shape[1]): if patch[row, col] > 100: bounds = draw_rectangle_from_coords(ax, row, col) print(bounds) bounds_list.append(bounds) # Get the min and max x and y values xmin = min([b[0] for b in bounds_list]) xmax = max([b[1] for b in bounds_list]) ymin = min([b[2] for b in bounds_list]) ymax = max([b[3] for b in bounds_list]) # Set the limits of the plot plt.xlim(xmin - 2, xmax + 1) plt.ylim(ymin - 1, ymax + 1) return fig, xmin, xmax, ymin, ymax def DMDPixelTransform(input_img, dmdMapping, xoffset=0, yoffset=0): # Initialize an array of zeros with same size as the input image transformed_img = np.zeros_like(input_img) # Get the dimensions of the input image rows, cols = input_img.shape # Iterate over the pixels of the input image for i in range(rows): for j in range(cols): # Calculate the new coordinates for the pixel ip = i + yoffset jp = j + xoffset # Apply the dmdMapping transformation if set if dmdMapping > 0: transformed_i = jp + ip - 2 transformed_j = (jp - ip + 4) // 2 else: transformed_i = ip transformed_j = jp # If the new coordinates are within the bounds of the image, copy the pixel value if 0 <= transformed_i < rows and 0 <= transformed_j < cols: transformed_img[transformed_i, transformed_j] = input_img[i, j] # Return the transformed image return transformed_img # Streamlit app st.title("ONI DMD emulator") # add author info and context st.markdown("Charles N. Christensen, ONI — 2023/06/14") tabs = st.tabs( [ "Basic rendering", "Pregenerated pattern with padding", "Manual pattern definition", ] ) with tabs[0]: st.header("Render DMD layout") # Create a new figure fig, ax = plt.subplots() # streamlit input for number of columns and rows cols = st.number_input("Number of columns", min_value=1, max_value=20, value=5) rows = st.number_input("Number of rows", min_value=1, max_value=20, value=5) bounds_list = [] for col in range(cols): for row in range(rows): bounds = draw_rectangle_from_coords(ax, row, col) bounds_list.append(bounds) # Get the min and max x and y values xmin = min([b[0] for b in bounds_list]) xmax = max([b[1] for b in bounds_list]) ymin = min([b[2] for b in bounds_list]) ymax = max([b[3] for b in bounds_list]) # Set the limits of the plot plt.xlim(xmin - 2, xmax + 1) plt.ylim(ymin - 1, ymax + 1) fig.set_size_inches(cols + 0.5, rows + 0.5) st.pyplot(fig) with tabs[1]: st.subheader("Upload pregenerated pattern or use default") # upload pattern image pattern = st.file_uploader("Upload pattern image", type=["tif", "tiff"]) # fallback if pattern is None: option = st.selectbox( "Select pattern", ( "patterns_spotSize_2_Nspots_5_dmdMapping_1.tif", "patterns_spotSize_5_Nspots_20_dmdMapping_1.tif", "patterns_spotSize_1_Nspots_5_dmdMapping_1.tif", "patterns_pixelsize_ratio_1_k2_200_func_square_wave_one_third_dmdMapping_1.tif", "patterns_pixelsize_ratio_1_k2_200_func_square_wave_one_third_dmdMapping_0.tif", "patterns_pixelsize_ratio_1_k2_80_func_square_wave_one_third_dmdMapping_1.tif", "patterns_pixelsize_ratio_1_k2_80_func_square_wave_one_third_dmdMapping_0.tif", "patterns_pixelsize_ratio_1_k2_20_func_square_wave_one_third_dmdMapping_1.tif", "patterns_pixelsize_ratio_1.8_k2_200_func_square_wave_one_third.tif", "patterns_pixelsize_ratio_1.8_k2_150_func_square_wave_one_third.tif", "patterns_pixelsize_ratio_1.8_k2_110_func_square_wave_one_third.tif", "patterns_pixelsize_ratio_1.6_k2_80.tif", ), ) cur_dir = os.path.dirname(os.path.abspath(__file__)) def_pattern = f"{cur_dir}/patterns/{option}" img = io.imread(def_pattern) st.markdown("""**No pattern uploaded**: Loading selected default image.""") st.markdown( "Note that `pixelsize_ratio > 1` and `_dmdMapping_0` indicate an assumption of ortholinear grid (no DMD layout correction). The `_dmdMapping_1` patterns are corrected for DMD." ) else: print("loading image", pattern) img = tiff.imread(pattern) st.subheader("Parameters") cols = st.columns(2) # streamlit number input for padding with cols[0]: x_padding = st.number_input( "Vertical padding", min_value=0, max_value=10, value=0 ) with cols[1]: y_padding = st.number_input( "Horizontal padding", min_value=0, max_value=10, value=0 ) # streamlit number input for global offset cols = st.columns(2) with cols[0]: x_offset_img = st.number_input( "Vertical image offset (change to test near edges of DMD)", min_value=0, max_value=30, value=10, ) with cols[1]: y_offset_img = st.number_input( "Horizontal image offset (change to test near edges of DMD)", min_value=0, max_value=30, value=20, ) cols = st.columns(2) with cols[0]: rows_img = st.number_input( "Number of rows", min_value=5, max_value=100, value=20 ) with cols[1]: cols_img = st.number_input( "Number of columns", min_value=5, max_value=100, value=20 ) cols = st.columns(2) with cols[0]: frame_idx = st.number_input( "Plot frame index start", min_value=0, max_value=400, value=0 ) with cols[1]: frame_count = st.number_input( "Plot frame index range", min_value=1, max_value=5, value=2 ) global_bounds = None # plot patches in two frames for i in range(frame_idx, frame_idx + frame_count): patch = img[ i, y_offset_img : y_offset_img + rows_img, x_offset_img : x_offset_img + cols_img, ] # add 1pixel padding in top to patch patch = np.pad( patch, ((x_padding, 0), (y_padding, 0)), "constant", constant_values=0 ) fig, xmin, xmax, ymin, ymax = plot_patch(patch) if global_bounds is None: global_bounds = xmin, xmax, ymin, ymax xmin, xmax, ymin, ymax = global_bounds plt.xlim(xmin - 2, xmax + 1) plt.ylim(ymin - 2, ymax + 1) fig.set_size_inches(xmax - xmin + 4, ymax - ymin + 4) st.subheader(f"Frame {i}") st.pyplot(fig) with tabs[2]: # 5x5 array of st checkboxes st.subheader("Select pixels to turn on in tilted coordinate system") grid = np.zeros((5, 5)) for i in range(5): cols = st.columns(5) for j in range(5): with cols[j]: # set value based on i,j = 0,0 1,0 0,1 1,1 value = False if i == 1 or i == 2: if j == 1 or j == 2: value = True grid[i, j] = st.checkbox(f"{i},{j}", value=value, key=f"{i},{j}") grid = 255 * grid # streamlit number input for global offset x_offset = st.number_input( "Vertical global offset (change to test near edges of DMD)", min_value=0, max_value=30, value=10, ) y_offset = st.number_input( "Horizontal global offset (change to test near edges of DMD)", min_value=0, max_value=30, value=20, ) global_bounds = None # plot with offsets of 1px in x and y for xoffset in range(0, 2): for yoffset in range(0, 2): patch = np.zeros((40, 40)) patch[x_offset : x_offset + 5, y_offset : y_offset + 5] = grid patch = DMDPixelTransform(patch, 1, xoffset, yoffset) fig, xmin, xmax, ymin, ymax = plot_patch(patch) if global_bounds is None: global_bounds = xmin, xmax, ymin, ymax xmin, xmax, ymin, ymax = global_bounds plt.xlim(xmin - 2, xmax + 1) plt.ylim(ymin - 2, ymax + 1) fig.set_size_inches(xmax - xmin + 4, ymax - ymin + 4) st.subheader(f"Frame with offset x={xoffset} and y={yoffset}") st.pyplot(fig)