from __future__ import print_function, unicode_literals, absolute_import, division import numpy as np import warnings import os import datetime from tqdm import tqdm from collections import defaultdict from zipfile import ZipFile, ZIP_DEFLATED from scipy.ndimage.morphology import distance_transform_edt, binary_fill_holes from scipy.ndimage.measurements import find_objects from scipy.optimize import minimize_scalar from skimage.measure import regionprops from csbdeep.utils import _raise from csbdeep.utils.six import Path from collections.abc import Iterable from .matching import matching_dataset, _check_label_array try: from edt import edt _edt_available = True try: _edt_parallel_max = len(os.sched_getaffinity(0)) except: _edt_parallel_max = 128 _edt_parallel_default = 4 _edt_parallel = os.environ.get('STARDIST_EDT_NUM_THREADS', _edt_parallel_default) try: _edt_parallel = min(_edt_parallel_max, int(_edt_parallel)) except ValueError as e: warnings.warn(f"Invalid value ({_edt_parallel}) for STARDIST_EDT_NUM_THREADS. Using default value ({_edt_parallel_default}) instead.") _edt_parallel = _edt_parallel_default del _edt_parallel_default, _edt_parallel_max except ImportError: _edt_available = False # warnings.warn("Could not find package edt... \nConsider installing it with \n pip install edt\nto improve training data generation performance.") pass def gputools_available(): try: import gputools except: return False return True def path_absolute(path_relative): """ Get absolute path to resource""" base_path = os.path.abspath(os.path.dirname(__file__)) return os.path.join(base_path, path_relative) def _is_power_of_2(i): assert i > 0 e = np.log2(i) return e == int(e) def _normalize_grid(grid,n): try: grid = tuple(grid) (len(grid) == n and all(map(np.isscalar,grid)) and all(map(_is_power_of_2,grid))) or _raise(TypeError()) return tuple(int(g) for g in grid) except (TypeError, AssertionError): raise ValueError("grid = {grid} must be a list/tuple of length {n} with values that are power of 2".format(grid=grid, n=n)) def edt_prob(lbl_img, anisotropy=None): if _edt_available: return _edt_prob_edt(lbl_img, anisotropy=anisotropy) else: # warnings.warn("Could not find package edt... \nConsider installing it with \n pip install edt\nto improve training data generation performance.") return _edt_prob_scipy(lbl_img, anisotropy=anisotropy) def _edt_prob_edt(lbl_img, anisotropy=None): """Perform EDT on each labeled object and normalize. Internally uses https://github.com/seung-lab/euclidean-distance-transform-3d that can handle multiple labels at once """ lbl_img = np.ascontiguousarray(lbl_img) constant_img = lbl_img.min() == lbl_img.max() and lbl_img.flat[0] > 0 if constant_img: warnings.warn("EDT of constant label image is ill-defined. (Assuming background around it.)") # we just need to compute the edt once but then normalize it for each object prob = edt(lbl_img, anisotropy=anisotropy, black_border=constant_img, parallel=_edt_parallel) objects = find_objects(lbl_img) for i,sl in enumerate(objects,1): # i: object label id, sl: slices of object in lbl_img if sl is None: continue _mask = lbl_img[sl]==i # normalize it prob[sl][_mask] /= np.max(prob[sl][_mask]+1e-10) return prob def _edt_prob_scipy(lbl_img, anisotropy=None): """Perform EDT on each labeled object and normalize.""" def grow(sl,interior): return tuple(slice(s.start-int(w[0]),s.stop+int(w[1])) for s,w in zip(sl,interior)) def shrink(interior): return tuple(slice(int(w[0]),(-1 if w[1] else None)) for w in interior) constant_img = lbl_img.min() == lbl_img.max() and lbl_img.flat[0] > 0 if constant_img: lbl_img = np.pad(lbl_img, ((1,1),)*lbl_img.ndim, mode='constant') warnings.warn("EDT of constant label image is ill-defined. (Assuming background around it.)") objects = find_objects(lbl_img) prob = np.zeros(lbl_img.shape,np.float32) for i,sl in enumerate(objects,1): # i: object label id, sl: slices of object in lbl_img if sl is None: continue interior = [(s.start>0,s.stop0,s.stop 0: # ignore image boundary, since predictions may not be reliable mask_b = np.zeros_like(mask) mask_b[b:-b,b:-b] = True else: mask_b = True points = np.nonzero(mask & mask_b) if prob is not None: # weighted sampling via prob w = prob[points[0],points[1]].astype(np.float64) w /= np.sum(w) ind = np.random.choice(len(points[0]), n_samples, replace=True, p=w) else: ind = np.random.choice(len(points[0]), n_samples, replace=True) points = points[0][ind], points[1][ind] points = np.stack(points,axis=-1) return points def calculate_extents(lbl, func=np.median): """ Aggregate bounding box sizes of objects in label images. """ if (isinstance(lbl,np.ndarray) and lbl.ndim==4) or (not isinstance(lbl,np.ndarray) and isinstance(lbl,Iterable)): return func(np.stack([calculate_extents(_lbl,func) for _lbl in lbl], axis=0), axis=0) n = lbl.ndim n in (2,3) or _raise(ValueError("label image should be 2- or 3-dimensional (or pass a list of these)")) regs = regionprops(lbl) if len(regs) == 0: return np.zeros(n) else: extents = np.array([np.array(r.bbox[n:])-np.array(r.bbox[:n]) for r in regs]) return func(extents, axis=0) def polyroi_bytearray(x,y,pos=None,subpixel=True): """ Byte array of polygon roi with provided x and y coordinates See https://github.com/imagej/imagej1/blob/master/ij/io/RoiDecoder.java """ import struct def _int16(x): return int(x).to_bytes(2, byteorder='big', signed=True) def _uint16(x): return int(x).to_bytes(2, byteorder='big', signed=False) def _int32(x): return int(x).to_bytes(4, byteorder='big', signed=True) def _float(x): return struct.pack(">f", x) subpixel = bool(subpixel) # add offset since pixel center is at (0.5,0.5) in ImageJ x_raw = np.asarray(x).ravel() + 0.5 y_raw = np.asarray(y).ravel() + 0.5 x = np.round(x_raw) y = np.round(y_raw) assert len(x) == len(y) top, left, bottom, right = y.min(), x.min(), y.max(), x.max() # bbox n_coords = len(x) bytes_header = 64 bytes_total = bytes_header + n_coords*2*2 + subpixel*n_coords*2*4 B = [0] * bytes_total B[ 0: 4] = map(ord,'Iout') # magic start B[ 4: 6] = _int16(227) # version B[ 6: 8] = _int16(0) # roi type (0 = polygon) B[ 8:10] = _int16(top) # bbox top B[10:12] = _int16(left) # bbox left B[12:14] = _int16(bottom) # bbox bottom B[14:16] = _int16(right) # bbox right B[16:18] = _uint16(n_coords) # number of coordinates if subpixel: B[50:52] = _int16(128) # subpixel resolution (option flag) if pos is not None: B[56:60] = _int32(pos) # position (C, Z, or T) for i,(_x,_y) in enumerate(zip(x,y)): xs = bytes_header + 2*i ys = xs + 2*n_coords B[xs:xs+2] = _int16(_x - left) B[ys:ys+2] = _int16(_y - top) if subpixel: base1 = bytes_header + n_coords*2*2 base2 = base1 + n_coords*4 for i,(_x,_y) in enumerate(zip(x_raw,y_raw)): xs = base1 + 4*i ys = base2 + 4*i B[xs:xs+4] = _float(_x) B[ys:ys+4] = _float(_y) return bytearray(B) def export_imagej_rois(fname, polygons, set_position=True, subpixel=True, compression=ZIP_DEFLATED): """ polygons assumed to be a list of arrays with shape (id,2,c) """ if isinstance(polygons,np.ndarray): polygons = (polygons,) fname = Path(fname) if fname.suffix == '.zip': fname = fname.with_suffix('') with ZipFile(str(fname)+'.zip', mode='w', compression=compression) as roizip: for pos,polygroup in enumerate(polygons,start=1): for i,poly in enumerate(polygroup,start=1): roi = polyroi_bytearray(poly[1],poly[0], pos=(pos if set_position else None), subpixel=subpixel) roizip.writestr('{pos:03d}_{i:03d}.roi'.format(pos=pos,i=i), roi) def optimize_threshold(Y, Yhat, model, nms_thresh, measure='accuracy', iou_threshs=[0.3,0.5,0.7], bracket=None, tol=1e-2, maxiter=20, verbose=1): """ Tune prob_thresh for provided (fixed) nms_thresh to maximize matching score (for given measure and averaged over iou_threshs). """ np.isscalar(nms_thresh) or _raise(ValueError("nms_thresh must be a scalar")) iou_threshs = [iou_threshs] if np.isscalar(iou_threshs) else iou_threshs values = dict() if bracket is None: max_prob = max([np.max(prob) for prob, dist in Yhat]) bracket = max_prob/2, max_prob # print("bracket =", bracket) with tqdm(total=maxiter, disable=(verbose!=1), desc="NMS threshold = %g" % nms_thresh) as progress: def fn(thr): prob_thresh = np.clip(thr, *bracket) value = values.get(prob_thresh) if value is None: Y_instances = [model._instances_from_prediction(y.shape, *prob_dist, prob_thresh=prob_thresh, nms_thresh=nms_thresh)[0] for y,prob_dist in zip(Y,Yhat)] stats = matching_dataset(Y, Y_instances, thresh=iou_threshs, show_progress=False, parallel=True) values[prob_thresh] = value = np.mean([s._asdict()[measure] for s in stats]) if verbose > 1: print("{now} thresh: {prob_thresh:f} {measure}: {value:f}".format( now = datetime.datetime.now().strftime('%H:%M:%S'), prob_thresh = prob_thresh, measure = measure, value = value, ), flush=True) else: progress.update() progress.set_postfix_str("{prob_thresh:.3f} -> {value:.3f}".format(prob_thresh=prob_thresh, value=value)) progress.refresh() return -value opt = minimize_scalar(fn, method='golden', bracket=bracket, tol=tol, options={'maxiter': maxiter}) verbose > 1 and print('\n',opt, flush=True) return opt.x, -opt.fun def _invert_dict(d): """ return v-> [k_1,k_2,k_3....] for k,v in d""" res = defaultdict(list) for k,v in d.items(): res[v].append(k) return res def mask_to_categorical(y, n_classes, classes, return_cls_dict=False): """generates a multi-channel categorical class map Parameters ---------- y : n-dimensional ndarray integer label array n_classes : int Number of different classes (without background) classes: dict, integer, or None the label to class assignment can be - dict {label -> class_id} the value of class_id can be 0 -> background class 1...n_classes -> the respective object class (1 ... n_classes) None -> ignore object (prob is set to -1 for the pixels of the object, except for background class) - single integer value or None -> broadcast value to all labels Returns ------- probability map of shape y.shape+(n_classes+1,) (first channel is background) """ _check_label_array(y, 'y') if not (np.issubdtype(type(n_classes), np.integer) and n_classes>=1): raise ValueError(f"n_classes is '{n_classes}' but should be a positive integer") y_labels = np.unique(y[y>0]).tolist() # build dict class_id -> labels (inverse of classes) if np.issubdtype(type(classes), np.integer) or classes is None: classes = dict((k,classes) for k in y_labels) elif isinstance(classes, dict): pass else: raise ValueError("classes should be dict, single scalar, or None!") if not set(y_labels).issubset(set(classes.keys())): raise ValueError(f"all gt labels should be present in class dict provided \ngt_labels found\n{set(y_labels)}\nclass dict labels provided\n{set(classes.keys())}") cls_dict = _invert_dict(classes) # prob map y_mask = np.zeros(y.shape+(n_classes+1,), np.float32) for cls, labels in cls_dict.items(): if cls is None: # prob == -1 will be used in the loss to ignore object y_mask[np.isin(y, labels)] = -1 elif np.issubdtype(type(cls), np.integer) and 0 <= cls <= n_classes: y_mask[...,cls] = np.isin(y, labels) else: raise ValueError(f"Wrong class id '{cls}' (for n_classes={n_classes})") # set 0/1 background prob (unaffected by None values for class ids) y_mask[...,0] = (y==0) if return_cls_dict: return y_mask, cls_dict else: return y_mask def _is_floatarray(x): return isinstance(x.dtype.type(0),np.floating) def abspath(root, relpath): from pathlib import Path root = Path(root) if root.is_dir(): path = root/relpath else: path = root.parent/relpath return str(path.absolute())