import requests as r from io import StringIO from Bio import SeqIO import xml.etree.ElementTree as ET def get_uniprot_seq(protein_id): print('>> Fetching UniProt Sequences for ID: ', protein_id) baseUrl = "http://www.uniprot.org/uniprot/" currentUrl = baseUrl + protein_id + ".fasta" response = r.post(currentUrl) cData = ''.join(response.text) Seq = StringIO(cData) pSeq = list(SeqIO.parse(Seq, 'fasta')) try: return str(pSeq[0].seq) except: IndexError return str('') def get_isoforms(protein_id): print('>> Fetching UniProt Isoforms for ID: ', protein_id) try: # a dictionary storing the sequence of your isoforms, key: accesion number, value: sequence isoforms = dict() # make a call to EBI API req = r.get('https://www.ebi.ac.uk/proteins/api/proteins/{}/isoforms'.format(protein_id)) # parse the returned XML uniprot = ET.fromstring(req.text) for isoform in uniprot: # get the sequence seq = isoform.find('{http://uniprot.org/uniprot}sequence') # get the accession number iso_accession = isoform.find('{http://uniprot.org/uniprot}accession') # add the values to the dictionary if seq.text and iso_accession.text: isoforms[iso_accession.text] = seq.text return isoforms except: AttributeError isoforms = {} return isoforms