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Biomed Res IntBiomed Res IntBMRIBioMed Research International2314-61332314-6141Hindawi 10.1155/2018/9163543Research ArticleInsecticide Resistance and Its Intensity in Populations of Malaria Vectors in Colombia http://orcid.org/0000-0001-7232-5056Orjuela Lorena I. stephanie89@example.net
1

2
http://orcid.org/0000-0002-9613-4083Morales Juliana A. 
3
http://orcid.org/0000-0002-5153-8383Ahumada Martha L. 
3
http://orcid.org/0000-0002-4562-7048Rios Juan F. 
4
González John J. 
5
Yañez Johana 
6
Rosales Angelo 
7
http://orcid.org/0000-0002-9214-3724Cabarcas Diana M. 
8
http://orcid.org/0000-0002-0492-1777Venegas Juan 
9
http://orcid.org/0000-0001-8081-4212Yasnot Maria F. 
1
http://orcid.org/0000-0001-5204-7693Quiñones Martha L. 
10

1Grupo de Investigaciones Microbiológicas y Biomédicas de Córdoba-GIMBIC, Universidad de Córdoba, Monteria 230001, Colombia
2Universidad de Cartagena, Facultad de Medicina, Sede Zaragocilla, Calle 30 N° 48-152, Cartagena de Indias, Bolivar, Cartagena 1300, Colombia
3Grupo de Entomología, Instituto Nacional de Salud, Bogotá D.C. 110111, Colombia
4Secretaria de Salud de Antioquía, Instituto Colombiano de Medicina Tropical, Medellín 050001, Colombia
5Residente del Programa de Epidemiología del Campo FETP, Instituto Nacional de Salud, Bogotá D.C. 110111, Colombia
6Instituto Departamental de Salud de Norte de Santander, Cúcuta 540001, Colombia
7Secretaria de Salud Distrital de Buenaventura, Buenaventura 764501, Colombia
8Secretaria de Salud Departamental de Córdoba, Monteria 230001, Colombia
9Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Santiago de Chile 8320000, Chile
10Departamento de Salud Pública, Universidad Nacional, Bogotá D.C. 110111, ColombiaAcademic Editor: Ademir J. Martins

2018 29 8 2018 2018 916354320 3 2018 31 5 2018 Copyright © 2018 Lorena I. Orjuela et al.2018This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Insecticide resistance in malaria vectors threatens malaria prevention and control efforts. In Colombia the three primary vectors, Anopheles darlingi, An. nuneztovari s.l., and An. albimanus, have reported insecticide resistance to pyrethroids, organophosphates, carbamates, and DDT; however, the insecticide resistance monitoring is not continuous, and the data on the prevalence of resistance is scarce and geographically limited. We describe the resistance levels and intensity of previously detected resistant populations among primary malaria vectors from the most endemic malaria areas in Colombia. The study was carried out in 10 localities of five states in Colombia. Bioassays were carried out following the methodology of CDC Bottle Bioassay using the discriminating concentration and in order to quantify the intensity the specimens were exposed to 2, 5, and 10X discriminating concentrations. Five insecticides were tested: deltamethrin, lambda-cyhalothrin, alpha-cypermethrin, permethrin, and DDT. The results provide evidence of low resistance intensity and resistance highly localized to pyrethroids and DDT in key malaria vectors in Colombia. This may not pose a threat to malaria control yet but frequent monitoring is needed to follow the evolution of insecticide resistance.

Universidad de CartagenaCOLCIENCIASNational Institutes of HealthCTIN2-2016
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1. Introduction
Since 2000, substantial progress has been made in fighting malaria. WHO estimates that between 2000 and 2015, malaria case incidence was reduced by 41% and malaria mortality rates by 62% [1, 2]. In 2016, there was an estimate of 216 million episodes of malaria and about 445,000 deaths globally, two-thirds of which were among children. Among the malaria-endemic countries in the WHO region of the Americas, Colombia contributed 15.3% of the total number of cases in 2016 and, in the last two years (2015-2016), the number of cases reported have doubled despite earlier reductions [3]. In relation to mosquito vectors, Anopheles albimanus, An. darlingi, and An. nuneztovari s.l. are widely distributed and are considered the primary vectors responsible for malaria transmission in most regions of the country [4].

In Colombia, the organochlorine insecticide DDT was intensively used for malaria control from 1947 until 1994 when its use was prohibited [5, 6]; since then, malaria vector control is supported mainly in the use of organophosphates and pyrethroid insecticides in indoor residual spraying (IRS) and pyrethroids in treated mosquito-nets (ITNs) in both impregnated nets and long lasting insecticidal nets (LLINs). Currently, the main insecticides used in Colombia for IRS are deltamethrin, lambda-cyhalothrin, and fenitrothion and, for ITNs, deltamethrin and alpha-cypermethrin are used. WHO has confirmed that prolonged use of insecticides has led to the development of resistance in malaria vectors against different insecticides used in public health. According to the 2017 World Malaria Report, 80% (61/76) of countries that provided surveillance data reported resistance to at least one insecticide class in one malaria vector from one collection site, and 65% (50/76) reported resistance to two or more insecticide classes [3].

Resistance monitoring in Colombia is done following WHO and CDC protocols, reporting insecticide resistance to pyrethroids, organophosphates, carbamates, and DDT, in the three primary malaria vectors [7–14]. According to current WHO resistance criteria (mortality rate <90%: resistance is confirmed) [1, 2], Fonseca-González [8] and INS (2015) reported deltamethrin, lambda-cyhalothrin, malathion, and DDT resistance in An. albimanus populations collected in the states of Antioquia, Valle del Cauca and Chocó. Studies by Fonseca-González et al. 2009b [10] and INS 2015 [12] documented insecticide resistance to lambda-cyhalothrin, deltamethrin, malathion, fenitrothion, and DDT in An. nuneztovari s.l. populations collected in Antioquia, Córdoba, Chocó, and Norte de Santander. An. darlingi collected in Antioquia, Chocó, Putumayo and Santander showed resistance to alpha-cypermethrin, deltamethrin, lambda-cyhalothrin, permethrin, fenitrothion, propoxur, and DDT [7, 9, 11, 12].

The Insecticide Resistance Surveillance Network (IRSN) in Colombia is led by the National Institute of Health and departmental entomology units, universities, research centers, and the Ministry of Health take part of this network. Work by IRSN has described the frequency, geographic distribution and in some cases the mechanisms responsible for the observed resistance. These data have been useful in decision-making. For example, when resistance to pyrethroids and DDT was identified in An. darlingi from Chocó in 2005-2006, IRS was promptly changed to organophosphate insecticide fenitrothion, to which no resistance had been detected [15].

Resistance frequency data obtained using the discriminating concentrations do not necessarily translate into efficacy rates in the field, data which is crucial to decisions required to deploy public health pesticides strategies [1, 2]. Consequently, WHO and CDC recently suggested that resistance phenotypes detected using the discriminating concentrations should be further assessed for their potential operational significance using methods developed to assess the resistance intensity in the same target vector population [1, 2, 16]. Furthermore, the data can be used to compare resistance levels between populations, monitor resistance evolution, and make operational decisions [1, 2]. To the best of our knowledge, there are no records evaluating resistance intensity in populations of resistant malaria vectors in the Americas.

Little is known about the mechanisms responsible for the resistance observed in these populations. Fonseca-González et al. [10] partially attributed the resistance to deltamethrin and lambda-cyhalothrin, observed in An. nuneztovari s.l. populations from Chocó and Norte de Santander, to the high mixed-function oxidase levels and the high levels of acetyl cholinesterase activity with the resistance to malathion in An. nuneztovari s.l. population of Antioquia, whereas for An. darlingi elevated levels of mixed-function oxidases and nonspecific esterases were associated with low mortalities against lambda-cyhalothrin and DDT from a population in Chocó [9]. Finally, a recent study developed by the National Institute of Health in the population of Anopheles darlingi from Tagachi, Quibdó-Chocó resistant to pyrethroids, found no evidence of the existence of mutations type kdr in the site L1014F [17].

Insecticide resistance is a problem of great concern and it needs to be monitored in order to maintain the efficacy of vector control operations in the field, even more when the countries in the Americas region have decided to achieve in 2020 the goal of reducing malaria morbidity by 40% or more, taking as a base the official figures of 2015 [18].

In this study, we describe the resistance levels and intensity of previously detected resistant populations among primary malaria vectors from the most malaria-endemic areas in Colombia.

2. Methods
2.1. Study Area
The study was carried out in 10 localities of five states in Colombia: (Antioquia, Córdoba, Chocó, Norte de Santander, and Valle del Cauca) (Figure 1), areas with the highest burden of malaria in the country [5]. The study sites were chosen to encompass a range of primary malaria vector distribution, taking into account the previous results regarding pyrethroids and DDT resistance and biochemical mechanisms, rates of malaria incidence, easy access by land or water, safety, and public health priority in terms of resistance monitoring given the history of insecticide use.

To evaluate geographic range of previously reported resistance along the Atrato river in Antioquia and Chocó [7], we evaluated three locations around Tagachi separated by 17 to 30 kilometers.

2.2. Collection of Anopheles Mosquitoes
Anopheles were captured using human landing catches following the WHO standard recommendations of biosecurity to minimize the risk of malaria transmission in field technicians [19]. Collections were completed outdoors between 18:00 and 23:00 hours. All mosquitoes were identified morphologically in the field using taxonomic keys available for Colombia [20]. Adult female mosquitoes were caught directly in the field.

2.3. Biological Test for Determining Insecticide Susceptibility
Bioassays were performed between May and November 2016 and in August 2017, on An. nuneztovari s.l., An. darlingi, and An. albimanus. The bioassays were carried out in each locality following the standard methodology of CDC Bottle Bioassay [21]. The method involved gently aspirating batches of 15–25 mosquitoes into BOECO GERMANY bottles with 250 ml capacity coated with the discriminating concentrations of the insecticides of interest to reach 100 mosquitoes (5-7 replicates with tests performed over one or more than one day) and two or more negative control bottles coated with acetone only. The number of dead or alive mosquitoes was recorded at fifteen-minute time-intervals until diagnostic time was reached. Temperature and humidity were recorded during exposure period and mortality rate scored at diagnostic time. After the diagnostic time exposure was reached in each bottle, surviving mosquitoes were transferred to a plastic container impregnated with triethylamine plaster to sacrifice them and stored separately from those that died postexposure. Taxonomic determination of all specimens was performed postbioassay due to presence of high Anopheles diversity. The solutions were prepared and the bottles coated according to the CDC protocol [21].

The insecticides, doses, and diagnostic times tested were for deltamethrin 12,5 μg/bottle/30 minutes; lambda-cyhalothrin 12,5 μg/bottle/30 minutes; alpha-cypermethrin 12,5 μg/bottle/30 minutes; permethrin 21,5 μg/bottle/30 minutes and DDT 100 μg/bottle/30 minutes. Previous resistance reports and their use for IRS or LLINs by the National Malaria Control program justified the choice of these insecticides. Quality control of each insecticide solution was performed at the National Institute of Health using the An. albimanus susceptible strain Cartagena. Discriminating concentrations and diagnostic time applied in the present study were the recommended by CDC [21]. When insufficient numbers of mosquitoes were collected from sites to test all insecticides, insecticides were prioritized by history of resistance and selection pressure in the last five years.

2.4. Biological Test to Determine the Insecticide Resistance Intensity
When the mortalities in field populations of Anopheles darlingi, An. albimanus and An. nuneztovari s.l. were below 98%, intensity assays were performed to quantify the intensity of resistance, following the recommendations of WHO and CDC [1, 2, 16]. Briefly, bottles were treated with 2, 5, and 10 times the diagnostic dose of insecticide. The diagnostic time was not altered.

2.5. Data Analyses
The bioassay results were corrected using the abbott formula when the control mortality was between 5 and 15% [22]. Mortality percentages and confidence limits (95%) were calculated for each insecticide in each locality and its susceptibility status or intensity of resistance were defined according to the CDC criteria. In bioassays with diagnostic dose, resistant populations were defined as displaying less than 90% mortality, possibly resistant populations between 90–98% mortality, and susceptible ones greater than 98% [23]. In the intensity assays, mortality of 98-100% at the 2X, 5X, or 10X concentrations indicates low, moderate, or high resistance intensity, respectively.

All tests were conducted in the field at temperatures ranging from 16.5°C to 34.1°C with relative humidity ranging from 57% to 90% (Tables 1, 2, and 3).

2.6. Ethical Considerations
Ethics approval from the ethical committee of the National Health Institute of Colombia was obtained. Writen informed consent was obtained from all the participants CTIN 2-2016, act No. 9 of May 20, 2016.

3. Results
3.1. Insecticide Susceptibility Assays
Tables 1, 2, and 3 show each wild Anopheles spp. and insecticide from each field site with the corresponding mortality rate, 95% confidence interval and resistance status. Mortality ranged from 3 to 10% in the control groups except in Pangui, where the mortality averaged 14% against alpha-cypermethrin and lambda-cyhalothrin.


An. albimanus showed susceptibility to pyrethroids, although for lambda-cyhalothrin the mortality was lower than 98% which is then interpreted as possible resistance. This species showed resistance to DDT.


An. darlingi showed differential susceptibility status to pyrethriods and DDT. In the northern locality, Buchadó, this species was susceptible to all insecticides tested. However, in the southern localities by the Atrato river, possible resistance and confirmed resistance are shown to DDT as well as pyretroids (Table 2), except in the locality of San Francisco de Tauchigado, where this species was susceptible to all tested insecticides.

The species An. nuneztovari s.l. was susceptible in most of the tested localities to DDT and pyrethroids, and only in two localities possible resistance was found. In Cordoba (municipality of Buenaventura), Pacific Coast region, possible resistance to DDT and deltamethrin was found, as well as in Santa Rosa (municipality of Zulia), Estern region, and also mortalities between 90% and 98% were found to DDT (Table 3).

3.2. Insecticide Resistance Intensity
Resistance intensity was evaluated in the populations defined as resistant or possibly resistant according to the insecticide susceptibility assay. In all cases, for the three species tested, specimens exposed to 2X the discriminating concentrations showed 100% mortality indicating low intensity of resistance; therefore, it was not necessary to perform assays at 5 and 10X the diagnostic concentrations (Table 4).

4. Discussion
This study provides evidence that key malaria vectors in Colombia, An. darlingi, An. albimanus, and An. nuneztovari s.l., were resistant to pyrethroids and DDT. This resistance was highly localized. Pyrethroids resistance in these same vectors was of low intensity, as 100% mortality was observed when exposed to 2X the discriminating insecticide concentrations. However, these results should be interpreted cautiously as the number of specimens evaluated by species and insecticide were all lower than 40 mosquitoes and 95% confidence intervals ranged from low to high intensity insecticide resistance.

This is the first study that aimed to follow up on previous reports of insecticide resistance and to evaluate resistance intensity. We have classified 10 areas and three vector species regarding the need to closely and routinely monitor insecticide susceptibility, i.e., sentinel sites. The evidence of persistent insecticide resistance implies that at least annual monitoring is necessary in these localities, while the program is distributing LLINs as the primary malaria vector control intervention. Given that IRS is also available and is performed with organophosphates and pyrethroids in the Pacific coast, the alternation of both LLINs and IRS with organophosphates could be a strategy to slow down the possible impact of pyrethroids, as recommended by WHO [15].

We observed changes in resistance compared to results previously obtained by the IRSN [7, 8, 12, 14]. The populations of An. darlingi from Tagachi and Bocas de Pune and the poulation of An. albimanus from Pangui continue to present results compatible with resistance to pyrethroids and DDT, although the percentages of mortality observed during this study were much higher (80.1%-97.8%) than those reported in previous studies (20.4 and 89%) [7, 8, 12]. These differences in mortality results may be due to the different genotype frequencies associated with the involved resistance mechanisms in the mosquitoes tested now compared with the mosquitoes tested in the past, as it has been found with kdr mutation in An. gambiae [24–26]. Alternatively, these higher mortality rates could be due to changes in selection pressure with insecticides. We find the population of An. nuneztovari s.l. from El Zulia to show resistance only to DDT in contrast with results found 11 years ago in this site, where resistance to lambda-cyhalothrin, fenithrotion, deltamethrin, and DDT was detected [10]. These changes towards susceptibility in this population could be explained by a decrease in the selective pressure with insecticides for malaria control, given the 88% reduction in the incidence of cases that has occurred in the last ten years [27, 28]. Another possible explanation is a decrease in the selective pressure due to lowered use of agricultural insecticides, given that the main economic activity in this area changed from rice crop to palm cultivation, a crop which is less pressured with insecticides due to lower pests' burden [29].

Additionally, we are the first to describe resistance data in populations of An. nuneztovari s.l. from Santa Isabel de Amará, Gallo, and Cordoba. The first two were susceptible to pyrethroids despite their wide use by the control program in indoor residual spraying with deltamethrin and lambda-cyhalothrin and LLINs with deltamethrin and alpha-cypermethrin. In Cordoba, we observed 94% and 95% mortality to DDT and deltamethrin, respectively, indicating that this malaria vector population should be carefully monitored as a priority in this state. This is of particular importance as this locality has been exerting continuous selective pressure with the scale-up with LLINs impregnated with alpha-cypermethrin and deltamethrin and IRS with deltamethrin.

The measurement of the relationship between insecticide resistance intensity and operational level control strategy failure has only recently been recommended [1, 2]. IRS failure has been observed when the levels of resistance intensity of the local vector populations are high [30]. This is less clear when using LLINs, attributed by some to the personal protection provided by the nets despite high level of vector resistance [31, 32]. WHO suggests interpreting resistance intensity assays as such that less than 98% mortality in mosquitoes exposed to 5 times and especially 10 times the discriminant concentration may indicate that control measures are ineffective in the field, indicating the urgency of developing adequate strategies for insecticide resistance management. According to the results found in this study, a low resistance intensity was observed among all tested species and insecticides pairs. It is probable that the low intensity of resistance detected does not yet pose a threat to malaria control in An. darlingi populations from Bocas de Pune, Tagachi and Encharcazon, in the population of An albimanus from Pangui, and in the population of An. nuneztovari s.l. from Cordoba. Nevertheless, given that Colombian vectorial control is based on distribution of LLINs (deltametrina or alpha-cypermethrin) and IRS (fenitrothion), we recommend at least annual resistance intensity surveillance for these three insecticides to develop timely strategies. This can prevent or delay increases in the intensity of resistance that may affect the operative control.

The evaluations of susceptibility made in the localities along the Atrato river, Bocas del Pune, Tauchigado, Tagachi, and Buchadó (<30 km distribution) demonstrated that resistance can be extremely localized, likely associated with variable insecticide use although it is difficult to determine given that there is no system that routinely reports these data. This localized variation highlights the importance of adapting local strategies for insecticide resistance management. Resistance to DDT, lambda-cyalothrin, deltamethrin, and permethrin was previously observed along the Atrato river in the locality of Amé-Beté [7, 9]. When pyrethroid resistance was detected, a recommendation was made to treat outbreaks and epidemics in this area with IRS with organophosphate fenitrothion instead of lambda-cyhalothrin and deltamethrin [33]. However, to date in Tagachi, both, organophosphates and pyrethroids are used in IRS and pyrethroids are used in LLINs. This can result in increased insecticide resistance to selective pressure. We could not follow up on this population in Amé-Beté during this study as this locality disappeared (personal communication JDP).

Despite the fact that DDT has not been used in Colombia since 1994, populations still remain resistant to DDT. It is possible that a genetic effect is still present in the population or that the subsequent and continuous pressures with pyrethroids are maintaining this cross-resistance. However, little is known about the mechanisms responsible for the resistance observed in these populations.

The taxonomic confirmation of the specimens used for insecticide resistance tests is necessary given the high diversity of species and the presence of species complexes. An. nuneztovari s.l., for example, is considered a complex formed by at least three species [34–36]. Nevertheless, despite the high intraspecific morphological variation observed in specimens collected throughout its range of distribution in Colombia, the evidence indicated that An. nuneztovari s.l. constitutes a unique species in Colombia [37–40], which corresponds to An. nuneztovari s.s. [41]. In this study, although molecular determinations were not performed, we suppose that An. nuneztovari s.s was the species tested. The results with this species suggest possible resistance to the insecticide DDT in Santa Rosa and to the insecticides deltametrina and DDT in Cordoba. Furthermore, these localities have been exerting continuous selection pressure with the scale-up in coverage with LLINs treated with alpha-cypermethrin and deltamethrin and IRS with deltamethrin, lambda-cyhalotrin, and fenitrothion. Therefore, we recommend prioritizing in these states the careful monitoring of this malaria vector population.

Despite efforts form the Ministry of Health, National Institute of Health and the support of PAHO/WHO to increase awareness and capacity building for a Colombian Insecticide Resistance Surveillance Network, data on the prevalence of resistance in malaria vectors is scarce and geographically limited. Research may be limited by logistical difficulties in accessing relevant malaria-endemic areas, or high species diversity.

To highlight this, we found changes in species composition in previous evaluated localities. This was the case of An. darlingi in the locality of Encharcazon, Rio Iro - Choco, where before this species was not present and the predominant vector was An. nuneztovari s.l. [10]. The presence of An. darlingi resistant to DDT in this area was also unexpected. According to the historical records of distribution [20] and personal communications with technicians who worked for the malaria eradication service, An. darlingi was not previously present along the San Juan River. Therefore, it is likely that this resistant population comes from a population of resistant An. darlingi located along the Atrato river. However, there is no evidence of whether An. darlingi is replacing An. nuneztovari s.l. in this area or if these two species are exhibiting seasonality. This information is critical to define periods of resistance assessments, allowing for adjustments according to species of interest and the epidemiology of the disease given that both An. nuneztovari s.l. and An. darlingi are primary vectors of malaria in the country.

This is the first time that resistance intensity has been measured in Colombia and to our knowledge in the Americas. This information is useful for the local decision makers. Future priorities are to (1) strengthen the existing National Surveillance Insecticide Resistance Network and (2) to follow up on identified priority sentinel sites to obtain information on the susceptibility status of the main vectors to insecticides and intensity of resistance over time.

5. Conclusion
The results presented here demonstrate that highly focalized and low-intensity insecticide resistance has been maintained in some populations of the primary malaria vectors in Colombia. This may not pose a threat to malaria control yet. In general An. darlingi, An. nuneztovari s.s., and An. albimanus were susceptible to three of the insecticides used in the country for malaria control, the pyrethroids: deltamethrin, lambda-cyhalothrin, and alpha-cypermethrin. In localities where resistance or possible resistance has been detected, routine surveillance is necessary to evaluate temporary changes. This will guide efficient use of vector control tools. It can also prevent unmanageable resistance levels that potentially compromise efficiency of malaria vectors control measures used in the field.

Acknowledgments
The first author acknowledges the support of the PhD program at the Universidad de Cartagena and COLCIENCIAS (announcement 647). This work was made with the support of the National Institute of Health (CTIN2-2016). Special thanks are due to the field assistants: Jose Dolores Palacios (JDP), Basilio, Alberto, Esperanza, Jhon, Estivinson, Mario, Andrés, Tito, Dario, José del Carmen, Edison, Luis, Anibal, Julio, Yorgi, Omar, Alfonso, Martha, Diana, Kathiana, Oscar, Ocaris, Rafael, and Norberto. The authors gratefully acknowledge Amy R Krystosik for a critical review of the manuscript.

Data Availability
The data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest
The authors declare that they have no conflicts of interest.

Authors' Contributions
Lorena I. Orjuela, Martha L. Ahumada, and Martha L. Quiñones conceived the idea and designed the study. Lorena I. Orjuela, Juliana A. Morales, Juan F. Rios, John J. González, Diana M. Cabarcas, Angelo Rosales, and Johana Yañez carried out field experiments. Lorena I. Orjuela performed species identification, analyzed the data, and wrote the paper. Juliana A. Morales, Martha L. Quiñones, Maria F. Yasnot, and Juan Venegas made critical revision of the manuscript regarding important intellectual content. All authors have read and approved the final manuscript.

Figure 1 Field sites in Colombia. Distribution of the three primary Anopheles species represented by colors (ALB: Anopheles albimanus, NTV: Anopheles nuneztovari s.l., and DAR: Anopheles darlingi). Black dots indicate sampling sites in the five states. These states are shown in black in the map of Colombia on the upper right panel. Colombia's location is shown in black in the South America map in the lower right panel.

Table 1 Average mortality rate, 95% confidence interval, and corresponding resistance interpretation (resistant (< 90% mortality), possibly resistant (90–98%), and susceptible (>98%)) among wild An. albimanus assayed via CDC bottle bioassays with discriminating concentrations.


Locality, Municipality 
(State)	
Temperature 
°C	
Relative humidity 
%	
Insecticide 
(concentration)	
n
	% mortality 
(95% CI)	
CDC Susceptibility status
	
 Pangui, Nuqui (Choco)	 24.29 - 29.7	 81 - >90	Alpha-cypermethrin
(12.5 ug/ml)	103	
100 
(96.4 - 100)	Susceptible	
Deltamethrin
(12.5 ug/ml)	109	
100 
(96.6 - 100)	Susceptible	
Lambda-cyhalothrin
(12.5 ug/ml)	109	
97.87 
(95.16 - 100)	Possibly resistant	
Permethrin
(21.5 ug/ml)	97	
100 
(96.19 - 100)	Susceptible	
DDT
(100 ug/ml)	101	
92.49 
(87.35 - 97.63)	Possibly resistant	
Table 2 Average mortality rate, 95% confidence interval, and corresponding resistance interpretation (resistant (< 90% mortality), possibly resistant (90–98%), and susceptible (>98%)) among wild An. darlingi assayed via CDC bottle bioassays with discriminating concentrations.


Locality, Municipality 
(State)	
Temperature 
°C	
Relative humidity 
%	
Insecticide 
(concentration)	
n
	% mortality 
(95% CI)	
CDC Susceptibility status
	
Buchado, Vigia del Fuerte (Antioquia) 	 24.1 - 27.8	 68 - 83	Alpha-cypermethrin
(12.5 ug/ml)	100	
100 
(96.3 - 100)	Susceptible	
Deltamethrin
(12.5 ug/ml)	164	
100 
(97.71 - 100)	Susceptible	
Lambda-cyhalothrin
(12.5 ug/ml)	101	
100 
(96.34 - 100)	Susceptible	
Permethrin
(21.5 ug/ml)	106	
100 
(96.5 - 100)	Susceptible	
DDT
(100 ug/ml)	101	
100 
(96.34 - 100)	Susceptible	


	
Bocas de Pune, Medio Atrato (Choco)	25.6 - 27.9	87 - >90	DDT
(100 ug/ml)	112	
90.7 
(85.32 - 96.08)	Possibly resistant	


	
San Francisco de Tauchigado, Medio Atrato (Choco) 	 24.5 - 26.1	 >90	Alpha-cypermethrin
(12.5 ug/ml)	110	
100 
(96,63 - 100)	Susceptible	
Deltamethrin
(12.5 ug/ml)	110	
100 
(96.63 - 100)	Susceptible	
Lambda-cyhalothrin
(12.5 ug/ml)	112	
100 
(96.68 - 100)	Susceptible	
Permethrin
(21.5 ug/ml)	106	
100 
(96.5 - 100)	Susceptible	
DDT
(100 ug/ml)	105	
100 
(96.47 - 100)	Susceptible	


	
 Tagachi, Quibdo (Choco)	 25.5 - 33.6	 65 - >90	Alpha-cypermethrin
(12.5 ug/ml)	101	
100 
(96.34 - 100)	Susceptible	
Deltamethrin
(12.5 ug/ml)	107	
95.16 
(91.09 - 99.23)	Possibly resistant	
Lambda-cyhalothrin
(12.5 ug/ml)	104	
96.92 
(93.59 - 100)	Possibly resistant	
Permethrin
(21.5 ug/ml)	109	
95.93 
(92.23 - 99.64)	Possibly resistant	
DDT
(100 ug/ml)	99	
80.13 
(72.27 - 87.99)	Resistant	


	
 Encharcazon, Rio Iro (Choco)	 27.5 - 34.1	 57 - 84	Deltamethrin
(12.5 ug/ml)	113	
100 
(95.86 - 100)	Susceptible	
DDT
(100 ug/ml)	93	
95.7 
(89.46 - 98.31)	Possibly resistant	
Table 3 Average mortality rate, 95% confidence interval, and corresponding resistance interpretation (resistant (< 90% mortality), possibly resistant (90–98%), and susceptible (>98%)) among wild An. nuneztovari s.l. assayed via CDC bottle bioassays with discriminating concentrations.


Locality, Municipality 
(State)	
Temperature 
°C	
Relative humidity 
%	
Insecticide 
(concentration)	
n
	% mortality 
(95% CI)	
CDC Susceptibility status
	
Santa Isabel de Amara, Segovia (Antioquia) 	 25.6 - 31.3	 59 - 84	Alpha-cypermethrin
(12.5 ug/ml)	98	
100 
(96.23 - 100)	Susceptible	
Deltamethrin
(12.5 ug/ml)	113	
100 
(96.71 - 100)	Susceptible	


	
Santa Rosa, El Zulia (Norte de Santander) 	 16.5 - 22.8	 <60	Alpha-cypermethrin
(12.5 ug/ml)	119	
100 
(96.87 - 100)	Susceptible	
Deltamethrin
(12.5 ug/ml)	103	
100 
(96.4 - 100)	Susceptible	
Lambda-cyhalothrin
(12.5 ug/ml)	106	
100 
(96.5 - 100)	Susceptible	
DDT
(100 ug/ml)	124	
97.43 
(94.65 - 100)	Possibly resistant	


	
Cordoba, Buenaventura (Valle del Cauca) 	 25.7 - 27.5	 78 - >90	Alpha-cypermethrin
(12.5 ug/ml)	112	
100 
(96.68 - 100)	Susceptible	
Deltamethrin
(12.5 ug/ml)	107	
94.95 
(92.83 - 97.06)	Possibly resistant	
Lambda-cyhalothrin
(12.5 ug/ml)	102	
100 
(96.37 - 100)	Susceptible	
Permethrin
(21.5 ug/ml)	101	
100 
(96.34 - 100)	Susceptible	
DDT
(100 ug/ml)	100	
94 
(87.52 - 97.22)	Possibly resistant	


	
 Gallo, Tierralta (Cordoba)	 24.2 - 29.2	 78 - 94	Alpha-cypermethrin
(12.5 ug/ml)	174	
100 
(97.84 - 100)	Susceptible	
Deltamethrin
(12.5 ug/ml)	146	
100 
(97.44 - 100)	Susceptible	
Lambda-cyhalothrin
(12.5 ug/ml)	152	
100 
(97.54 - 100)	Susceptible	
Permethrin
(21.5 ug/ml)	141	
100 
(97.35 - 100)	Susceptible	
DDT
(100 ug/ml)	120	
100 
(96.9 - 100)	Susceptible	
Table 4 Average mortality rates, 95% confidence interval, and corresponding resistance intensity interpretation among wild Anopheles spp. assayed via CDC bottle intensity bioassays with 2x discriminating concentrations.


Anopheles specie
	
Locality, Municipality 
(State)	
Temperature 
°C	
Relative humidity 
%	
Insecticide 
(concentration)	
n
	% mortality 
(95% CI)	
Intensity of resistance
	

An. albimanus
	Pangui, Nuqui (Choco)	24.29 - 29.7	81 - >90	DDT
(200 ug/ml)	21	100
(84.54 - 100)	Low intensity	


	

An. darlingi
	Bocas de Pune, Medio Atrato (Choco)	25.6 - 27.9	87 - >90	DDT
(200 ug/ml)	38	100
(90,82 - 100)	Low intensity	
 Tagachi, Quibdo (Choco)	 25.5 - 33.6	 65 - >90	Deltamethrin
(25 ug/ml)	30	100
(88.65 - 100)	Low intensity	
Lambda-cyhalothrin
(25 ug/ml)	25	100
(86.68 - 100)	Low intensity	
Permethrin
(43 ug/ml)	31	100
(88.97 - 100)	Low intensity	
DDT
(200 ug/ml)	30	100
(88.65 - 100)	Low intensity	


	

An. nuneztovari s.l.
	Cordoba, Buenaventura (Valle del Cauca) 	 25.7 - 27.5	 78 - >90	Deltamethrin
(25 ug/ml)	28	100
(87.94 - 100)	Low intensity	
DDT
(200 ug/ml)	25	100
(86.68 - 100)	Low intensity
==== Refs
1 World Health Organization Geneva: World Malaria Report 2016, CC BY-NC-SA 3.0 IGO [cited 2017], http://apps.who.int/iris/bitstream/10665/252038/1/9789241511711-eng.pdf?ua=1  
2 World Health Organization Test Procedures for Insecticide Resistance Monitoring in Malaria Vector Mosquitoes  2016 2nd Geneva, switzerland 
3 World Health Organization Geneva: World Malaria Report 2017, CC BY-NC-SA 3.0 IGO [cited 2018], http://apps.who.int/iris/bitstream/10665/259492/1/9789241565523-eng.pdf?ua=1  
4 Montoya-Lerma J.  Solarte Y. A.  Giraldo-Calderón G. I.    Malaria vector species in Colombia: a review Memórias do Instituto Oswaldo Cruz  2011 106 1 223 589-84-0006/S0074-02762011000900028 2-s2.0-80051761076 21881778 
5 Padilla J. C.  Alvarez G.  Montoya R.  Chaparro P.  Herrera S.   Epidemiology and control of malaria in Colombia Memorias do Instituto Oswaldo Cruz  2011 106 supplement I 114 122 21881765 
6 ica.gov.co Colombia: Restricciones, prohibiciones y suspensión de registros de plaguicidas de uso agricola en Colombia [cited 2017], http://www.ica.gov.co/getdoc/b2e5ff99-bd80-45e8-aa7a-e55f0b5b42dc/PLAGUICIDAS-PROHIBIDOS.aspx  
7 Suarez M. F.  Quiñones M. L.  Palacios J. D.  Carillo A.   First record of DDT resistance in Anopheles darlingi  Journal of the American Mosquito Control Association  1990 6 1 72 74 2324727 
8 Fonseca-González I.   Estatus de la resistencia a insecticidas de los vectores primarios de malaria y dengue en Antioquia, Chocó, Norte de Santander y Putumayo, Colombia [Ph.D. Thesis]  2008 Universidad de Antioquia, Medellin 
9 Fonseca-González I.  Quiñones M. L.  McAllister J.  Brogdon W. G.   Mixed-function oxidases and esterases associated with cross-resistance between DDT and lambda-cyhalothrin in Anopheles darlingi  Root 1926 populations from Colombia Memórias do Instituto Oswaldo Cruz  2009 104 1 18 26 10.1590/S0074-02762009000100003 2-s2.0-62549111310 19274371 
10 Fonseca-González I.  Cárdenas R.  Quiñones M. L.  McAllister J.  Brogdon W. G.   Pyrethroid and organophosphates resistance in Anopheles (N.) nuneztovari  Gabaldón populations from malaria endemic areas in Colombia Parasitology Research  2009 105 5 1399 1589-84-0006/s00436-009-1570-2 2-s2.0-70349602946 19655174 
11 Santacoloma L.  Tibaduiza T.  Gutiérrrez M.  Brochero H.   Susceptibility to insecticides of Anopheles darlingi  Root 1840, in two locations of the departments of Santander and Caquetá, Colombia Biomédica  2012 32 22 28 10.7705/biomedica.v32i0.455 23235810 
12 Instituto Nacional de Salud Colombia: Informe final vigilancia de susceptibilidad a insecticidas de Anopheles (Nyssorhynchus) darlingi, An. (N.) nuneztovari y An. (N.) albimanus  en localidades centinelas de los departamentos de Antioquia, Cauca, Choco, Córdoba y Valle del Cauca. Año 2009 – 2014 [cited 2016], http://www.ins.gov.co/temas-de-interes/Memorias%20Malaria/10.Resistencia%20a%20insecticidas.pdf  10.30854/anf.v25.n44.2018.396 
13 Quiñones M. L.  Norris D. E.  Conn J. E.    Insecticide resistance in areas under investigation by the international centers of excellence for malaria research: a challenge for malaria control and elimination The American Journal of Tropical Medicine and Hygiene  2015 93 3 69 78 10.4269/ajtmh.14-0844 2-s2.0-84965084073 26259947 
14 Instituto Nacional de Salud Colombia: Informe epidemiológico 'Red de vigilancia de la resistencia a insecticidas de uso en salud pública en Colombia 2004-2014' [cited 2016], http://www.ins.gov.co/lineas-de-accion/Red-Nacional-Laboratorios/Referente%20Nacional%20de%20la%20Vigilancia%20de%20la%20resisten/Informe%20VRI%20%202004-2014.pdf  10.17533/udea.rfnsp.v34n2a06 
15 World Health Organization Global Plan for Insecticide Resistance Management in Malaria Vectors  2012 Geneva, Switzerland 
16 Centers for Disease Control and Prevention Insert 2 - Enhanced Surveillance Protocol for the CDC Intensity Bottle Bioassay – 2013. Guideline for Evaluating Insecticide Resistance in Vectors Using the CDC Bottle Bioassay Atlanta: Centers for Disease Control and Prevention: pp. 3, {{PHONE+SOCIAL_SECURITY_NUMBER}}/ICE.567.555.9187 
17 Instituto Nacional de Salud Colombia: Vigilancia de la resistencia a los insecticidas y perspectivas futuras para su manejo [cited 2017], http://www.paho.org/hq/index.php?option=com_docman&amp;task=doc_view&amp;gid=34883&amp;Itemid=270&amp;lang=es  
18 Pan American Health Organization Washington D.C.: Plan de acción para la eliminación de la malaria 2016 – 2020 [cited 2018], http://www.paho.org/hq/index.php?option=com_docman&task=doc_view&gid=35669&Itemid=270&lang=es  
19 World Health Organization Catches off bait Manual on Practical Entomolgy in Malaria  1975 Part II. Methods and Techniques Geneva, Switzerland WHO Division of Malaria and Other Parasitic Diseases 
20 González R.  Carrejo N.   Introducción al Estudio Taxonómico de Anopheles de Colombia Claves y Notas de Distribución  2009 2nd Santiago de Cali: Programa Editorial de la Universidad 
21 Centers for Disease Control and Prevention Guidelines for evaluating insecticide resistance in vectors using the CDC bottle bioassay Atlanta: Centers for Disease Control and Prevention: pp. 28, 2010 
22 Abbott W. S.   A method of computing the effectiveness of an insecticide Journal of Economic Entomology  1925 18 2 265 589-84-0006/jee/18.2.265a 
23 Centers for Disease Control and Prevention Insert 1 - Revised Box 5: Interpretation of data for resistance management purposes – 2012. Guideline for Evaluating Insecticide Resistance in Vectors Using the CDC Bottle Bioassay Atlanta: Centers for Disease Control and Prevention: pp. 1, 2012 
24 Reimer L.  Fondjo E.  Patchoké S.    Relationship between kdr mutation and resistance to pyrethroid and DDT insecticides in natural populations of Anopheles gambiae Journal of Medical Entomology  2008 45 2 260 266 2-s2.0-41049090589-84-0006/0022-2585(2008)45[260:RBKMAR]2.0.CO;2 10.1093/jmedent/45.2.260 18402142 
25 Ramphul U.  Boase T.  Bass C.  Okedi L. M.  Donnelly M. J.  Müller P.   Insecticide resistance and its association with target-site mutations in natural populations of Anopheles gambiae  from eastern Uganda Transactions of the Royal Society of Tropical Medicine and Hygiene  2009 589-84-0006 1589-84-0006/j.trstmh.2009.02.014 2-s2.0-70349808481 19303125 
26 Donnelly M. J.  Corbel V.  Weetman D.  Wilding C. S.  Williamson M. S.  Black W. C. IV   Does kdr genotype predict insecticide-resistance phenotype in mosquitoes? Trends in Parasitology  2009 25 5 213 219 2-s2.0-65249157589-84-0006/j.pt.2009.02.007 19369117 
27 Instituto Nacional de Salud Colombia: Informe final malaria en Colombia 2007, Informe final 2007, subdirección de vigilancia y control en salud pública, Colombia 2007, [cited 2017], http://www.ins.gov.co/lineas-de-accion/Subdireccion-Vigilancia/Informe%20de%20Evento%20Epidemiolgico/ETV%202007.pdf  
28 Instituto Nacional de Salud Colombia: Informe del evento malaria, hasta el periodo epidemiológico XII, Colombia 2016, [cited 2017], http://www.ins.gov.co/lineas-de-accion/Subdireccion-Vigilancia/Informe%20de%20Evento%20Epidemiolgico/MALARIA%20PERIODO%20XIII%202016.pdf  
29 Ministerio del Medio Ambiente Lineamientos de politica ambiental para el uso y manejo de plaguicidas, Colombia 2008, [cited 2018], https://www.cortolima.gov.co/SIGAM/poli/plaga.doc  
30 Venter N.  Oliver S. V.  Muleba M.    Benchmarking insecticide resistance intensity bioassays for Anopheles malaria vector species against resistance phenotypes of known epidemiological significance Parasites & Vectors  2017 10 1 198 589-84-0006/s13071-017-2134-4 2-s2.0-85019015105 28427447 
31 Strode C.  Donegan S.  Garner P.  Enayati A. A.  Hemingway J.   The impact of pyrethroid resistance on the efficacy of insecticide-treated bed nets against african anopheline mosquitoes: systematic review and meta-analysis PLoS Medicine  2014 11 3 e1001589-84-0006/journal.pmed.1001619 2-s2.0-84898999488 
32 Bradley J.  Ogouyèmi-Hounto A.  Cornélie S.    Insecticide-treated nets provide protection against malaria to children in an area of insecticide resistance in Southern Benin Malaria Journal  2017 16 1 2-s2.0-85019736495 
33 Organización Panamericana de la Salud Reorientación y fortalecimiento de la prevención y el control de la malaria en el departamento del Chocó, costa pacífica colombiana 2006-2009 Bogotá, pp. 87, 2589-84-0006/biomedica.v26i3.350 
34 Conn J.  Puertas Y. R.  Seawright J. A.   A new cytotype of Anopheles nuneztovari from western Venezuela and Colombia. Journal of the American Mosquito Control Association  1993 9 3 294 301 2-s2.0-0027654261 8245938 
35 Scarpassa V. M.  Geurgas S.  Azeredo-Espin A. M. L.  Tadei W. P.   Genetic divergence in mitochondrial DNA of Anopheles nuneztovari  (Diptera: Culicidae) from Brazil and Colombia Genetics and Molecular Biology  2000 23 1 71 78 10.1590/S1415-47572000000100013 2-s2.0-0034099968 
36 Mirabello L.  Conn J. E.   Population analysis using the nuclear white gene detects Pliocene/Pleistocene lineage divergence within Anopheles nuneztovari  in South America Medical and Veterinary Entomology  2008 22 2 109 589-84-0006/j.1365-2915.2008.00731.x 2-s2.0-44349117889 18498609 
37 Sierra D. M.  Velez I. D.  Linton Y.-M.   Malaria vector Anopheles (Nyssorhynchus) nuneztovari  comprises one genetic species in Colombia based on homogeneity of nuclear ITS2 rDNA Journal of Medical Entomology  2004 41 3 302 589-84-0006/0022-2585-41.3.302 2-s2.0-2542615369 15185929 
38 Naranjo-Díaz N.  Sallum M. A. M.  Correa M. M.   Population dynamics of Anopheles nuneztovari in Colombia Infection, Genetics and Evolution  2016 45 56 65 2-s2.0-84993953589-84-0006/j.meegid.2016.08.019 27553709 
39 Ahumada M. L.  Orjuela L. I.  Pareja P. X.    Spatial distributions of Anopheles species in relation to malaria incidence at 70 localities in the highly endemic Northwest and South Pacific coast regions of Colombia Malaria Journal  2016 15 1 1 16 2-s2.0-84992092991 10.1186/s12936-016-1421-4 26729363 
40 Ruiz F.  Linton Y.-M.  Ponsonby D. J.    Molecular comparison of topotypic specimens confirms Anopheles (Nyssorhynchus) dunhami Causey (Diptera: Culicidae) in the Colombian Amazon Memórias do Instituto Oswaldo Cruz  2010 105 7 899 903 2-s2.0-78649366589-84-0006/S0074-02762010000700010 21120360 
41 Scarpassa V. M.  Cunha-Machado A. S.  Saraiva J. F.   Evidence of new species for malaria vector Anopheles nuneztovari sensu lato in the Brazilian Amazon region Malaria Journal  2016 15 1 10.1186/s12936-016-1217-6 2-s2.0-84966263475


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