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Cell Death DisCell Death DisCell Death & Disease2041-4889Nature Publishing Group cddis201533610.1038/cddis.2015.33626583320CorrespondenceHSP70 protects human neuroblastoma cells from apoptosis and oxidative stress induced by amyloid peptide isoAsp7-Aβ(1–42) CorrespondenceYurinskaya M M 12Mitkevich V A 1Kozin S A 1Evgen'ev M B 1Makarov A A 1*Vinokurov M G 21 Laboratory of Protein Conformational Polymorphism in Health and Disease, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia2 Laboratory of Apoptosis Regulation, Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow, Russia* Laboratory of Protein Conformational Polymorphism in Health and Disease, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street 32, Moscow 119991, Russia. Tel: 902.475.8418; Fax: 902.475.8418; E-mail: james83@example.org 2015 19 11 2015 1 11 2015 6 11 e1977  Copyright © 2015 Macmillan Publishers Limited2015Macmillan Publishers LimitedCell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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Dear Editor,

The initial stage of Alzheimer's disease (AD) is characterized by aggregation of monomeric amyloid-β (Aβ) and accumulation of Aβ as insoluble amyloid plaques,1 which are in dynamic equilibrium with soluble oligomers of Aβ.2 Formation of neurotoxic oligomers most likely proceeds via a nucleation-dependent mechanism and is initiated by structurally and/or chemically modified forms of Aβ.3 The Aβ isoform with isomerized aspartic acid residue at position 7 (isoAsp7-Aβ(1–42)) is one of the most common in amyloid plaques. In contrast to intact Aβ, a synthetic peptide corresponding to isoAsp7-Aβ(1–42) causes cerebral amyloidosis in AD animal models4 and induces neuronal cell death by apoptosis.5 These findings point to isoAsp7-Aβ(1–42) as an agent triggering the initial step of AD – oligomerization of endogenous Aβ.1 Oxidative stress has a significant role in the development of AD,6 leading to an increase in the major stress protein HSP70. We have previously shown that recombinant human HSP70 reduces oxidative stress in innate immunity cells,7 prevents death of neurons and reduces amyloid plaque burden in the brain of 5xFAD mice when administered intranasally.8 This study examines the protective effect of recombinant human HSP70 on apoptosis and generation of reactive oxygen species (ROS) in human neuroblastoma cells, SK-N-SH, induced by isoAsp7-Aβ(1–42).

In the concentration range of 1–10 μM, isoAsp7-Aβ(1–42) induced a dose-dependent increase in the proportion of apoptotic cells and production of ROS (Figures 1a and b). However, the proportion of necrotic cells in the population treated with isoAsp7-Aβ(1–42) increased by no more than 6% compared with the control. Predominant cell death by the apoptosis pathway indicates a specific effect of isoAsp7-Aβ(1–42), and the mechanism of this effect is associated with the induction of oxidative stress. Preincubation of the cells with HSP70 resulted in a significant decrease in the proportion of apoptotic cells and the level of ROS induced by isoAsp7-Aβ(1–42) (Figure 1b). We have not detected any interaction between the isoAsp7-Aβ(1–42) and HSP70 by ITC and MST (data not shown). In addition, in experiments with cells the concentration of HSP70 (30 nM) was significantly lower than that of isoAsp7-Aβ(1–42). Thus, the protective effect of HSP70 could not be attributed to the interaction with isoAsp7-Aβ(1–42).

We have investigated the impact of various inhibitors of intracellular signaling pathways that regulate apoptosis and oxidative stress on the effect of isoAsp7-Aβ(1–42). We used wortmannin, U73122, PD98059, SB203580 and SP600125 for inhibition of PI3K, PLC, ERK, p38MAPK and JNK, correspondingly.

Although isoAsp7-Aβ(1–42) increased the level of ROS and the percentage of apoptotic cells, all inhibitors reduced these parameters in SK-N-SH cells (Figure 1c). This indicates that isoAsp7-Aβ(1–42) causes a response in the cells, linked to activation of the intracellular signaling pathways mediated by JNK, ERK, PI3K, p38MAPK and PLC. Although U73122 had no effect on the HSP70 protection against isoAsp7-Aβ(1–42), SB203580 reduced the protective effect of HSP70 against apoptosis, but did not affect the level of ROS. Shutting down protein kinases JNK, ERK and PI3K completely eliminated the HSP70 protective effect against oxidative stress and apoptosis induced by isoAsp7-Aβ(1–42). These data suggest that the observed protective effects of HSP70 are due to the activity of protein kinases JNK, ERK and PI3K.

In conclusion, we have demonstrated for the first time that the protective effect of HSP70 is realized via two main mechanisms – reduction of the oxidative stress and apoptosis induced by the peptide isoAsp7-Aβ(1–42) in human neuroblastoma cells. Signaling pathways involving protein kinases JNK, ERK and PI3K have a key role in these mechanisms. It is reasonable to believe that the protective effect of HSP70 against isoAsp7-Aβ(1–42) can also be observed at the level of the whole organism, and used as an approach for the prevention of AD by utilizing recombinant HSP70.

The study was funded by the Russian Science Foundation (grant #14-24-00100).

The authors declare no conflict of interest.

Figure 1 Protective effect of HSP70 against apoptosis and production of ROS induced by isoAsp7-Aβ(1–42) in neuroblastoma cells SK-N-SH. Neuroblastoma cells SK-N-SH were cultured in RPMI-1640 medium supplemented with the 10% heat-inactivated defined fetal calf serum (FCS), 2 mM l-glutamine, 100 units/ml of penicillin and 100 μg/ml streptomycin at 37 °C in an atmosphere with 5% CO2. Before 24 h of treatment, the culture medium was replaced with the medium without FCS. Viability of the cells used in the experiments was 98–99%. Human recombinant LPS-free HSP70 expressed in armyworm (Spodoptera) cells was used.7 Synthetic peptide isoAsp7-Aβ(1–42) was purchased from Biopeptide and prepared as described in ref. 5. (a) Visualization of the cells using inverted fluorescence microscope Keyence BZ8100. Left, untreated cells. Center, cells treated with isoAsp7-Aβ(1–42) (5 μM, 24 h). Right, cells preincubated with HSP70 (30 nM, 2 h), and subsequently treated with isoAsp7-Aβ(1–42) (5 μM, 24 h). Scale length 20 μm. Arrows indicate: 1, necrotic cells visualized by propidium iodide (PI); 2, apoptotic cells visualized by Hoechst 33342; 3, living cells. (b) Effect of different concentrations of isoAsp7-Aβ(1–42) and 30 nM of HSP70 on the level of ROS (left) and the amount of apoptotic cells (right) in a population of SK-N-SH cells. 1, cells treated with isoAsp7-Aβ(1–42) (24 h). 2, cells preincubated with HSP70 (30 nM, 2 h), and then treated with isoAsp7-Aβ(1–42) (24 h). The level of ROS in cells was determined by nitro blue tetrazolium: intracellular formazan was dissolved in 2 M KOH and DMSO, absorbance was measured at 620 nm. Percent of apoptotic cells was calculated as a fraction of PI-negative cells with fragmented DNA to the total number of cells (100%). To register apoptosis at least 20 fields of view were analyzed, each of which contained 250–350 cells. (c) The effect of inhibitors of signaling pathways on the level of ROS (left) and amount of apoptotic cells (right) in a population of SK-N-SH cells treated with HSP70 and isoAsp7-Aβ(1–42). C, control; SP, inhibitor of c-Jun N-terminal kinase (JNK), SP600125 (20 μM); PD, inhibitor of extracellular signal-regulated kinase (ERK), PD 98059 (10 μM); Wort, inhibitor of phosphoinositide-3-kinase (PI3K), wortmannin (100 nM); SB, inhibitor of p38 mitogen-activated protein kinase (p38MAPK), SB203580 (10 μM), U, inhibitor of phospholipase C (PLC), U73122 (1 μM). The inhibitors of signaling pathways were added to cells 30 min before treatment with isoAsp7-Aβ(1–42) and HSP70. 1, untreated cells; 2, cells treated with isoAsp7-Aβ(1–42) (24 h). 3, cells preincubated with HSP70 (30 nM, 2 h), and then treated with isoAsp7-Aβ(1–42) (5 μM, 24 h). Each value is the mean of at least six independent experiments with triplicate samples±S.D. The comparison of data groups was performed using Student's t-test; n=6, P<0.005
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