Table S2. Collection and sampling information for polar bears from the Western and Southern Hudson Bay subpopulations. These data have been previously published, and are replicated here for clarity (WHB females were not included in previous publication) (Morris et al. 2017). Sample Code Age Sex Age class Capture date Subpopulation Capture location (Nunavut) 7 F Adult 21-Feb-2014 Southern Hudson Bay Sanikiluaq K14-39072 3 F Subadult 15-Feb-2014 Southern Hudson Bay Sanikiluaq K14-39073 3 F Subadult 21-Feb-2014 Southern Hudson Bay Sanikiluaq K14-39081 7 F Adult 18-Feb-2014 Southern Hudson Bay Sanikiluaq K14-39082 5 F Adult 18-Feb-2014 Southern Hudson Bay Sanikiluaq K14-39085 4 F Subadult 24-Feb-2014 Southern Hudson Bay Sanikiluaq K14-39086 6 F Adult 15-Feb-2014 Southern Hudson Bay Sanikiluaq K14-39087 3 F Subadult 15-Feb-2014 Southern Hudson Bay Sanikiluaq K14-39088 5 F 15-Feb-2014 Southern Hudson Bay Sanikiluaq K14-39069 8 M 18-Feb-2014 Southern Hudson Bay Sanikiluaq K14-39070 5 M Adult 18-Feb-2014 Southern Hudson Bay Sanikiluaq K14-39071 8 M Adult 18-Feb-2014 Southern Hudson Bay Sanikiluaq K14-39074 3 M Adult 20-Feb-2014 Southern Hudson Bay Sanikiluaq 18 M Adult 15-Feb-2014 Southern Hudson Bay Sanikiluaq K14-39076 6 M 15-Feb-2014 Southern Hudson Bay Sanikiluaq 4 M Subadult 24-Feb-2014 Southern Hudson Bay Sanikiluaq
S4. Results of target organohalogen (PCBs, OCs, PBDEs/HFRs) analyte compliance (%) to concentrations in standard reference materials (SRM, NIST 1945c organics in whale blubber) extracted with each batch of samples (n = 5). Analytes Min Max Mean ± SD Analytes Min Max Mean ± SD (%) (%) (%) (%) (%) (%) PCBs PCBs CB18 86.1 101 94.0 ± 5.7 CB146 112 121 117 ± 3.3 CB31/28 92.8 104 97.6 ± 5.4 CB149 106 116 109 ± 4.2 CB44 126 145 133 ± 6.8 CB151 97.5 109 102 ± 4.5 CB49 102 111 107 ± 3.7 CB153 77.7 82.6 80.2 ± 1.8 CB52 109 112 111 ± 1.4 CB156 103 117 112 ± 5.6 CB66 116 134 127 ± 7.6 121 144 134 ± 8.4 CB87 117 127 121 ± 4.1 128 133 131 ± 2.2 CB92 77.7 82.2 80.3 ± 2.0 CB183 114 118 116 ± 1.7 CB95 107 118 113 ± 4.9 CB187 122 127 125 ± 1.9 CB99 88 95.2 92.4 ± 2.8 CB194 116 120 118 ± 1.7 CB105 102 114 109 ± 4.4 CB195 79.4 88.5 82.1 ± 3.7 CB110 105 111 108 ± 2.7 13.6 15.4 14.3 ± 0.8 119 122 121 ± 1.4 CB206 119 132 127 ± 5.1 CB128 103 113 108 ± 3.8 CB209 109 120 114 ± 4.8 CB138/158 95.2 99.6 97.1 ± 1.8 OCs OCs
cis-Chlordane 115 118 116 ± 1.3 Hexachlorobenzene 138 148 144 ± 3.8 91.8 100 95.2 ± 3.4 -HCH 106 117 111 ± 4.0 p,p'-DDT 60.4 91 81.7 ± 12.3 -HCH ND ND ND p,p'-DDD 49 85.8 74.6 ± 14.8 Mirex 152 163 156.8 ± 4.4 p,p'-DDE 108 111 110 ± 1.3 cis-Nonachlor 112 116 113.9 ± 1.1 Dieldrin 113 120 116 ± 3.4 trans-Nonachlor 95.6 105 100.5 ± 3.4 Heptachlor epoxide 108 112 111 ± 1.9 Oxychlordane 95.3 118 106.3 ± 9.1 BDE47 98.4 106 102.5 ± 3.2 BDE99 94.5 118 109.9 ± 9.2 BDE100 94.1 113 103.2 ± 8.1 BDE153 106.2 121 115.2 ± 6.2 BDE154 116 131 124.7 ± 5.8 S5. Results of target analyte extractions from surrogate reference materials of spiked pork fat or liver (PFASs) (n = 5–6). Analytes were spiked into samples and extracted with batches of samples to monitor extractions and matrix effects when appropriate standard reference materials were unavailable. Compounds indicated with an asterisk (*) are considered on a semi-quantitative basis only. PCN recoveries were from a single laboratory control (extraction matrix) sample from ALS Analytical. Analyte Min Max Mean ± SD Analyte Min Max Mean ± SD Analyte Recovery (%) (%) (%) (%) (%) (%) (%) OPEs PFASs PCNs
TEP 90 101 95.2 ± 3.7 PFBA 81.3 87.1 84.2 ± 2.1 CN2 66 TDClPP 96 123 108 ± 12.5 PFPeA 93.1 106 99.8 ± 5.1 CN6/12 96 V6* 28 60 41.2 ± 10.8 PFHxA 92.9 103 96.9 ± 4.0 CN13 95 TDBPP 86 106 97.8 ± 7 PFHpA 98.5 104 100 ± 2 CN42 102 TMPP (TCrP) 89 99 93.3 ± 3.9 PFOA 94.1 101 97.4 ± 2.8 CN36/45 82 TTBPP* 49 73 62.5 ± 8.8 PFNA 95.1 106 99.7 ± 4.6 CN28/43 99 T2B4MP* 49 59 54 ± 4.3 PFDA 95.3 106 100 ± 5 CN30/27/39 95 T3B4MP* 27 38 33.5 ± 5 PFUdA 89.7 105 97.0 ± 5.5 CN48/35 95 T4B3MP* 25 38 33.2 ± 5.3 PFDoA 97.1 104 99.8 ± 2.4 CN46 100 TEHP 74 99 87 ± 8.9 PFTrDA 61.4 71.9 66.7 ± 3.8 CN52/60 101 TCEP 90 113 103 ± 7.6 PFTeDA 78.5 86.6 83.4 ± 3.1 CN50 103 TPhP 82 102 95.5 ± 7.2 PFHxDA 71.7 102 85.2 ± 11.0 CN54 104 TNBP 90 107 101 ± 5.6 PFODA 88.4 119 103 ± 12 CN53/55 105 90 122 103 ± 11.2 PFEtCHxS 29.9 62 44.3 ± 11.8 CN66/67 98 TPrP 90 109 99.2 ± 6.6 PFBS 87.8 95.3 93.1 ± 3.1 CN64/68 97
TPHPb 29 0.210 24 0.361 38 0.140 29 0.110 TCIPPb 13 < MLOD 6 < MLOD TPP 0 < MLOD 0 < MLOD TBOEPb 38 0.0830 35 0.0951 EHDPP 0 < MLOD 0 < MLOD PCBs CB16/32 Tri- 0 < MLOD 0 < MLOD CB17 Tri- 0 < MLOD 0 < MLOD CB18 Tri- 0 < MLOD 0 < MLOD CB33/20 Tri- 0 < MLOD 0 < MLOD CB22 Tri- 0 < MLOD 0 < MLOD CB31/28 Tri- 25 < MLOD 24 < MLOD CB64/41 Tetra- 0 < MLOD 6 < MLOD CB42 Tetra- 0 < MLOD 0 < MLOD CB44 Tetra- 0 < MLOD 12 < MLOD CB47/48 Tetra- 100 19.6 100 28.0 CB49 63 0.235 41 0.236 CB52 Tetra- 88 1.02 59 0.532 CB56/60 Tetra- 0 < MLOD 0 < MLOD CB66 88 1.17 53 0.254 CB70/76 Tetra- 0 < MLOD 0 < MLOD CB74 Tetra- 100 10.4 100 8.26 CB85 96 14.6 100 20.0 CB87 Penta- 0 < MLOD 0 < MLOD CB101/90 Penta- 0 < MLOD 6 < MLOD CB92 Penta- 42 < MLOD 24 < MLOD CB95 Penta- 4 < MLOD 12 < MLOD
CB97 Penta- 0 < MLOD 12 < MLOD CB99 Penta- 100 350 100 444 CB105 Penta- 100 14.4 100 12.4 CB110 0 < MLOD 0 < MLOD CB114 Penta- 100 2.09 100 1.89 CB118 Penta- 100 44.9 100 41.8 CB128 Hexa- 100 9.25 100 7.52 CB130 Hexa- 100 3.37 100 2.89 CB137 Hexa- 100 36.1 100 34 Hexa- 100 270 100 239 CB141 Hexa- 4 < MLOD 12 < MLOD CB146 Hexa- 100 56.5 100 46 CB149 Hexa- 92 1.19 94 1.46 CB151 Hexa- 8 < MLOD 41 < MLOD CB153 Hexa- 100 1667 100 1828 Hexa- 100 50.6 100 52.7 CB157 Hexa- 100 34.4 100 38.1 CB167 Hexa- 100 1.77 100 1.39 CB170/190 Hepta- 100 380 100 358 CB171 100 3.42 100 1.93 CB172 Hepta- 100 6.21 100 3.63 CB174 Hepta- 13 < MLOD 18 < MLOD Hepta- 0 < MLOD 0 < MLOD CB177 83 0.774 59 0.386 Hepta- 100 5.64 100 3.36 CB179 Hepta- 0 < MLOD 18 < MLOD
Hepta- 100 820 100 766 CB183 Hepta- 100 42.2 100 26.7 CB187 Hepta- 100 17.8 100 12.4 CB189 Hepta- 100 14.6 100 11 CB194 Octa- 100 175 100 131 CB195 Octa- 100 5.6 100 2.77 CB196/203 Octa- 100 27.1 100 12.2 CB199 Octa- 100 8.57 100 4.28 CB200 Octa- 21 < MLOD 0 < MLOD CB201 Octa- 0 < MLOD 0 < MLOD CB202 Octa- 100 4.38 100 1.81 CB205 Octa- 100 12.6 100 7.35 CB206 Nona- 100 72.1 100 37 CB207 Nona- 25 < MLOD 0 < MLOD CB208 Nona- 71 0.48 6 < MLOD Deca- 100 20.3 100 9.2 PCB 4335 100 4295 OCs/OCPs cis-Chlordane 100 10.8 100 12.1 trans-Chlordane 0 <MLOD 0 <MLOD cis-Nonachlor 100 4.02 100 4.85 trans-Nonachlor 100 144 100 175 Heptachlor epoxide 100 153 100 188 Oxychlordane 100 1213 100 1186 CHL 100 1549 100 1618
p,p'-DDT 100 4.75 88 2.08 p,p'-DDD 63 0.685 47 0.607 p,p'-DDE 100 145 100 66.1 DDT 151 100 70 Dieldrin 100 155 100 234 Endosulfan 0 < MLOD 0 < MLOD Endosulfan 0 < MLOD 0 < MLOD Endosulfan sulfate 0 < MLOD 12 < MLOD HCBD 0 < MLOD 0 < MLOD α-HCH 100 54.2 100 42.0 β-HCH 100 147 100 231 γ-HCH 21 < MLOD 6 < MLOD HCH 204 100 276 1,2,3,4-TCB 8 < MLOD 6 < MLOD 1,2,4,5-TCB 100 68.9 100 87.7 PeCBz 100 22.1 100 36.1 HCB 100 88.3 100 154 CBz 183 100 298 Octachlorostyrene 100 19.9 100 21.4 Mirex 92 15.2 76 5.25 Photomirex 100 58.1 100 54.1 Mirex 76.7 65.5 PCP 0 < MLOD 0 < MLOD PCA 0 < MLOD 0 < MLOD o,p'-dicofol 0 < MLOD 0 < MLOD p,p'-dicofol 0 < MLOD 0 < MLOD PBDEs BDE3 Mono- 0 < MLOD 0 < MLOD BDE7 Mono- 0 < MLOD 0 < MLOD
C12Cl5 Penta- 100 0.917 100 1.14 C12Cl6 Hexa- 100 3.86 100 4.81 C12Cl7 100 6.68 100 8.32 C12Cl8 Octa- 100 2.99 100 3.72 C12Cl9 Nona- 100 0.841 100 1.05 C12Cl10 Deca- 100 0.0895 100 0.111 C13Cl5 Penta- 100 0.349 100 0.435 C13Cl6 Hexa- 100 0.516 100 0.643 C13Cl7 Hepta- 100 0.454 100 0.565 C13Cl8 Octa- 100 0.161 100 0.200 C13Cl9 Nona- 100 0.0377 100 0.0469 C13Cl10 Deca- 0 < MLOD 0 < MLOD C SCCP 48.4 100 60.3 C SCCP 71.4 100 88.9 C SCCP 15.4 100 19.1 C SCCP 1.52 100 1.89 SCCP 137 100 170 PCNs CN2 Mono- 100 0.0243 80 0.0235 CN1 Mono- 80 0.00834 40 < MLOD
PFHxDA 63 0.0231 59 0.0239 PFODA 25 < MLOD 12 < MLOD PFCA 776 527 PFSAs PFEtCHxS 96 0.767 88 0.163 PFBS 54 0.0395 53 0.0174 100 8.28 100 7.01 PFOS 100 1191 100 794 PFDS 88 0.220 71 0.136 PFSA 1202 804 FASAs FBSA 0 < MLOD 12 < MLOD FOSA 100 4.98 100 5.64 N-MeFOSA 0 < MLOD 0 < MLOD N-EtFOSA 0 < MLOD 0 < MLOD ΣPFAS 1991 1343 a PFASs are wet weight (ww) concentrations, all other analytes were normalized to lipid weight (lw). b Because these are the first reports of OPEs in Arctic biota, and polar bears in particular, we have reported the geometric means and 95 % CI for all positive detections over 20 % DF were included.