11-Sep-12 Training room Inoculation room & LML BHSL-1 BHSL-2 Lab 6 Lab 2&3 Meeting room 8:30 to 9:00 Introduction 9:00 to 9:15 Assessment 9:15 to 10:15 L1:Overview of Animal tissue culture Lead: Dr Sudha Gangal 10:15 to 11:00 L 2:Tissue culture media Lead: Dr Alpana Moghe 11:00 to 11:30 Tea 11:30 to 12:00 Batch 1 D1:Cell culture lab Lead: Ketaki Batch 2 P1:Pipetting Lead: 3 video 12:00 to 12:30 Batch 2 D1:Cell culture lab Lead: Ketaki Batch 3 P1:Pipetting Lead: Shama Batch 1 12:30 to 13:00 Batch 3 D1:Cell culture lab Lead: Ketaki Batch 1 P1:Pipetting Lead: Shama 2 video 13:00 to 14:00 break 14:00 to 14:30 Batch 1 of cells Lead: Jayashree Batch 2 Lead: Ketaki Batch 3 Lead: Shama 14:30 to 15:00 Batch 1 P5: Subculture monolayer P6:Coverslip culture Lead: Jayashree Batch 2 of cells Lead: Ketaki Batch 3 VC Tour 15:00 to 15:30 Batch 2,3 Tea break 15:30 to 16:00 Batch 3 D4:Replicate culture & viable cell Lead: Shama Batch 2 cells in Lead: Ketaki 16:00 to 16:30 16:30 to 17:00 Batch 3 P7:Growth curve-Viable cell count Lead: Shama 17:00 to 17:30 1 Tea break 12-Sep-12 9:00 to 10:00 L3:Characterization of cell lines - I Lead: Dr Avinash Bhisey 10:00 to 10:30 Tea 10:30 to 11:00 3 2 suspension
Lead: Ketaki D3:Sterilization by filtration Lead: Jayashree D4:Replicate culture & viable cell count Lead: Shama 11:00 to 11:30 Batch 2 P5: Subculture monolayer P6:Coverslip culture Lead: Jayashree Batch 3 cells in Lead: Ketaki 11:30 to 12:00 Batch 1 P7:Growth curve-Viable cell count Lead: Shama 12:00 to 12:30 12:30 to 13:00 Batch 1 VC Tour 13:00 to 13:30 13:00 to 14:00 Lunch break Batch 1,3 13:30 to 14:30 Lunch break Batch 2 Batch 1 cells in suspension culture Lead: 14:00 to 14:30 Batch 3 P5: Subculture monolayer P6:Coverslip culture Lead: Jayashree Batch 2 D4:Replicate culture & viable cell count Lead: Ketaki 14:30 to 15:00 15:00 to 15:30 Batch 2 P7:Growth curve-Viable cell count Lead: Ketaki 15:30 to 16:00 Batch 1 D2:Sterilization autoclave Lead: 16:00 to 16:30 Batch 1,2 Tea break 16:30 to 17:00 Batch 3 Tea break Batch 1 by filtration Lead: Shama Batch 2 VC Tour 13-Sep-12 9:00 to 10:00 L4:Characterization of cell lines – II Lead: Dr Avinash Bhisey 10:00 to 11:00 L5:Specialized applications - Stem cell cultures Lead: Dr Ramesh Bhonde 11:00 to 11:30 Tea break 11:30 to 12:15 embryo culture Lead: Dr Savita Datar 12:15 to 13:15 Lunch break 13:15 to 14:30 1,2,3 on
embryo culture Lead: Dr Datar 14:30 to 15:00 Tea break 14:45 to 17:30 Tour of cell repository 14-Sep-12 9:00 to 10:00 L7:Organ and Organotypic cultures Lead: Dr Padma Shastry 10:00 to 11:00 L8:Applications of cell culture technique and cell behavior Lead: Dr Ulhas Wagh 11:00 to 11:30 Tea break 11:30 to 12:30 L9:Specialized applications - Monoclonal antibodies Lead: Dr Sudha Gangal 13:30 to 14:30 Lunch break 14:30 to 15:30 L10:Large scale production of cultured cells Lead: Dr Rajeev Dhere 15:30 to 16:00 Tea break 16:00 17:00 Batch 1,2, 3 P8:Giemsa staining Lead: Ketaki 15-Sep-12 9:00 to 10:00 Lecture 11: Primary cultures and its applications Lead: Dr Alpana Moghe 10:00 to 11:00 Tea break 11:00 to 12:00 Batch1,2,3 embryo culture Lead: Dr 12:00 to 13:00 break 14:30 to 15:00 Growth curve construction and analysis 15:00 to 15:30 Feedback and certificate distribution Course Director Dr. Sudha Gangal Former Research Director, children's Hospital, Mumbai Former Head, Immunology Division, Cancer Research Institute, Tata Memorial Centre, Mumbai