Supplementary Table 2. List of primer sequences used to generate the ΔackA-ptaΔyqhD strain (SE002) Knockouts Primer Sequence (5' to 3') ackA-pta Sense CGTAGTGATCGATGAGTCTGTTATTCAGGGTATCAAAGGTGTAGGCTGGAGCTGCTTC Antisense CAATCCCTGCACCCAGTTCTACACCCTGAGACGCTGATTCCGGGGATCCGTCGACC yqhD Sense CGCCATCATGGCGGTGCGGCGCTGCCTTCCAGTTCGGTTAACACGGTGTAGGCTGGAGCTGCTTC Antisense GCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATTCCGGGGATCCGTCGACC
Supplementary Table 3. List of the CoA transferases and acetyl-CoA synthetases (ACS) from the various microorganisms examined in this study. Plasmid Enzyme Origin UniProt ID pETDuet-1 Expression vector pSE_Tr_1 Acetate CoA-transferase (YdiF) Clostridium propionicum Q9L3F7 pSE_Tr_2 Acetate CoA-transferase (YdiF) Acetobacterium woodii H6LGM4 pSE_Tr_3 Acetate CoA-transferase (YdiF) Escherichia coli P37766 pSE_Tr_4 Acetate CoA-transferase (YdiF) Cupriavidus necator Q0K874 pSE_Tr_5 Acetyl CoA synthetase (ACS) Escherichia coli P27550 pSE_Tr_6 Acetyl CoA synthetase (ACS) Saccharomyces cerevisiae Q01574 pSE_Tr_7 Acetyl CoA synthetase (ACS) Saccharomyces cerevisiae P52910 pSE_Tr_8 Propionate-CoA ligase (PCL) Salmonella typhimurium P55912 pSE_Tr_9 Propionate-CoA ligase (PCL) Ruegeria pomeroyi Q5LPB4 pSE_Tr_10 Acetate CoA-transferase (YdiF) Burkholderia phytofirmans B2TEP1 pSE_Tr_11 Acetate CoA-transferase (YdiF) Desulfosporosinus youngiae H5XW98 pSE_Tr_12 Acetate CoA-transferase (YdiF) Bacillus halodurans Q9K634 pSE_Tr_13 Acetate CoA-transferase (YdiF) Desulfosporosinus meridiei J7IQ54 pSE_Tr_14 Acetate CoA-transferase (YdiF) Desulfosporosinus orientis G7W6H1 pSE_Tr_15 Acetate CoA-transferase (YdiF) Peptostreptococcus anaerobius R5JK89
pSE_De_54 Enoyl-CoA hydratase Chloroflexus aurantiacus B9LGG2 pSE_De_55 Enoyl-CoA hydratase Pelagibaca bermudensis Q0FVF4 pSE_De_56 Enoyl-CoA hydratase Nitratireductor aquibiodomus I5BQY5 pSE_De_57 Enoyl-CoA hydratase Loktanella vestfoldensis A3V1Q7 pSE_De_58 Oleate hydratase Elizabethkingia meningoseptica C7DLJ6 pSE_De_59 Oleate hydratase Streptococcus pyogenes Q5XDG8 pSE_De_60 Oleate hydratase Rhodopseudomonas palustris Q6N2E0 pSE_De_61 Oleate hydratase Holdemania filiformis B9YB92 pSE_De_62 2-hydroxyglutaryl-CoA dehydratase Acidaminococcus fermentans P11569 pSE_De_63 2-hydroxyglutaryl-CoA dehydratase Carboxydothermus hydrogen Q3ADT6 pSE_De_64 2-hydroxyglutaryl-CoA dehydratase Oscillibacter valericigenes G4KW63 pSE_De_65 2-hydroxyglutaryl-CoA dehydratase Desulfosporosinus orientis G7W6H3 pSE_De_66 2-hydroxyglutaryl-CoA dehydratase Peptostreptococcus anaerobius R5J4B7 pSE_De_67 2-hydroxyisocaproyl-CoA dehydratase Peptoclostridium difficile Q188I1
Supplementary Table 5: List of the CoA hydrolases derived from the various microorganisms examined in this study. Plasmid Enzyme Origin UniProt ID pETDuet-1 Expression vector pSE_Hy_1 Acyl-CoA thioester hydrolase Escherichia coli P0A8Z0 pSE_Hy_2 Acyl-CoA thioester hydrolase Klebsiella oxytoca H3M3Y5 pSE_Hy_3 Acyl-CoA thioester hydrolase Cronobacter turicensis C9XTQ3 pSE_Hy_4 Acyl-CoA thioester hydrolase Citrobacter freundii K8QWQ9 pSE_Hy_5 Acyl-CoA thioester hydrolase Salmonella enterica B5N634 pSE_Hy_6 Acyl-CoA thioester hydrolase Shigella flexneri I6H5N7 pSE_Hy_7 Acyl-CoA thioester hydrolase Heamophilus influenzae P44679