Part IX. Procedures for Use of Anesthetic Gases 41 Part X: Procedures for Use of Glass Bead Sterilizer 46 Part XI: Delegation of Authority for ARF Staff 48 ARF Floor Plan 50 IACUC References and Appendices 51 IACUC References 52 Appendix A: Contamination Control Procedures 53 Appendix B: Badge Monitoring . 54 Appendix C: Guidelines for Multiple Major Survival Surgeries 55 Appendix D: Guidelines for Anesthetics and Analgesics Use in Rodents 56
Dose (milligrams per kilogram) Route Comments Cervical dislocation N/A N/A Acceptable with prior sedation or anesthesia; exceptions must be approved by IACUC Decapitation N/A N/A Acceptable with prior sedation or anesthesia; if unable to sedate or anesthetize because need chemical residue-free tissues, head should be immediately frozen in liquid nitrogen subsequent to severing; exceptions must be approved by IACUC Exsanguinations N/A N/A Acceptable when preceded by methods that relieve anxiety, consciousness RATS Tranquilizers Agent Dose (milligrams per kilogram) Comments Telazol (Tiletamine HCl/Zolazepam HCl) 40 20-60 IP IM For chemical restraint; for light anesthesia Acetylpromazine (Acepromazine) 0.5 IM Diazepam (Valium) 3-5 IM/IP Ketamine (Ketaset, Vetalar) 50-100 IM/IP General Anesthetics Agent Dose (milligrams per kilogram) Route Comments Choralose 55 IP Scientific justification needed; terminal studies only Ketamine/Xylazine (Rompun) mixture Ketamine 40-80 mg Xylazine 5-10 mg IM/IP Make a mixture by adding 0.15 ml Xylazine (100 mg/ml stock solution) to each ml of ketamine (100 mg/ml stock solution); maximal anesthetic effect is achieved within 15 minutes and lasts 15-30 minutes Mixtures with Diazepam and Ketamine Ketamine 40-60 Diazepam 5-10 IP Make a mixture by adding 0.15 ml Xylazine (100 mg/ml stock solution) to each ml of ketamine (100 mg/ml stock solution); maximal anesthetic effect is achieved within 15 minutes and lasts 15-30 minutes Telazol (Tiletamine HCl, Zolazepam HCl) 20-60 40 IM IP For chemical restraint; for light anesthesia Thiopental (Pentothal) 20 40 IV IP For chemical restraint
Telazol (Tiletamine HCl, Zolazepam HCl) NOT RECOMMENDED General Anesthetics Agent Dose (milligrams per kilogram) Route Comments Alpha Choralose-1% solution 120 80-100 IV IV Use only for “physiological” preparations; Scientific justification needed Ketamine 20-60 15-20 IM IV Heart rate, respiratory rate, and blood pressure are depressed; FSH is suppressed (see under tranquilizers) Mixture of Ketamine and Diazepam Ketamine 60 Diazepam 5-10 IM rate, respiratory rate, and blood pressure are depressed; FSH is suppressed (see under tranquilizers) Ketamine and Xylazine (Rompun) 25-50 5-10 SC Duration of surgical anesthesia minutes) is dose-dependent; analgesia adequate for procedures Pentobarbital 20-50 IV Narrow margin of safety; administer dilute (1-3%) slowly to effect; more concentrated solutions or injections can cause vessel damage and lead to occlusion and necrosis; duration of anesthesia is 30-60 minutes but complete recovery may require 1-10 hours; peak effect of the IP dosage reached in 5-7 minutes; light surgical anesthesia lasts about 120 minutes and complete recovery may take 12-20 hours Isoflurane Induction 2-3% Maintenance 0.25-2% Inhalation Use a non-breathing system; nonflammable and nonexplosive; can be used for euthanasia Analgesics Agent Dose (milligrams per kilogram) Comments Ketoprofen 1.0 IM Every 12-24 hours Buprenorphine 0.1-0.05 IM, SC, or IV Every 6-12 hours Butorphanol 0.1-0.5 SC, IV Every 2-4 hours Morphine 2-5 SC, IM Every 2-4 hours Meloxicam 0.2 PO, SC Every 12-24 hours Miscellaneous