extraction of DNA V01 10 4 1-4 2 Final Visit/Visit 02 Sample Type Tube Type Number of Tubes Supplied in Kit Processing/ Aliquoting Tubes Shipped to Coriell Retained at Site Tubes Shipped to Local Lab for RNA extraction 2.5 ml PAXgeneTM Tube 3 N/A 2 1 0 Whole-blood: for isolation of plasma/pellet 2 ml microcentrifuge 18 1 ml plasma aliquots in each 2 ml microcentrifuge tube 9 0-9 0 10ml Lavender Top EDTA Tube 3 Retain blood pellet in EDTA tube 2 1 0 CSF 50 ml conical Tube 1 Combine and mix total CSF N/A 15 ml conical tubes 2 Divide and spin CSF 2 ml microcentrifuge tubes 18 1 ml CSF aliquots in each 2 ml microcentrifuge tube 10 0-8 0 2 ml purple-top microcentrifuge tubes 2 1 ml CSF aliquots in each 2 ml purple-top microcentrifuge tube 0 0 2 TOTAL V02 47 23 2-19 2 The following samples will be collected according to the visit schedule noted above. Blood for standard clinical safety analysis to isolate plasma suitable for proteomic, metabolomic and other analyte studies and pellets of cells for protein analyses. Whole blood for appropriate DNA and RNA analysis Cerebrospinal fluid
Data Recording Food Intake, Date __________ Pre-Lab Time _______ Pre-LP Time_________ Low-Fat Diet Menu Yes / No / Fasted PD Med Last Intake, Date __________ Time __________ Sample Time at Collection Volume Obtained (mL) Time at Centrifugation Time at Aliquoting No. of Aliquots Time at Freezing Freezing Method Dry Ice -80°C Freezer Visit 01 PT/PTT Ambient CBC/platelets Ambient Plasma/Pellet DNA Ambient Visit 02 RNA Date Time Plasma/Pellet CSF
Low-Fat Diet Menu Suggestions Due to the interference of lipid content in blood specimens collected for biomarker evaluation in the BIOFIND study, it is strongly advised that samples be collected after an 8 hour fast (no food or drink except fluids such as water, tea, black coffee). If fasting is not achievable, a subject should be on a low-fat diet for at least 8 hours prior to blood collection. Below is a list of suggested sample menus that could be consumed prior to blood collection. These lists are not all inclusive and sites should use their best judgment in this process. Foods that are allowed prior to blood collection: Sample Breakfast Items: Dry toast Fruit salad – no dressing tea or coffee (no milk or cream) Fruit or vegetable juice Dry cereal – (without nuts/ no granola; no milk) tea or coffee (no milk or cream) Fruit or vegetable juice oatmeal or other cooked whole grain cereal Topped with fresh or dried fruit (no butter, milk or cream) tea or coffee (no milk or cream) Fruit or vegetable juice Dry whole-wheat toast Poached egg-whites or egg substitute Clear tea or coffee (no milk or cream) Fruit or vegetable juice Sample Lunch Items: Turkey breast sandwich on whole wheat bread Lettuce and Tomato and Mustard Clear beverage Flavored gelatin Plain pasta with plain marinara sauce– no butter or cheese Side of steamed vegetables or green salad Clear beverage Flavored gelatin Steamed chicken breast (lean, without skin) Side of steamed vegetables or green salad Clear beverage Flavored gelatin Large tossed green salad with assorted vegetables (no dressing or cheese) Clear beverage Flavored gelatin Cucumber sandwich on whole-wheat bread Lettuce, tomatoes, shredded carrots, onions or other vegetables Clear beverage Flavored gelatin Clear broth with vegetables and pasta Fruit salad – no dressing Clear beverage Flavored gelatin Low-Fat Diet Menu Suggestions (continued)