Haematology Tests available at the Rotunda Hospital Haematology Department Active Date: 9th February 2018 Contact phone number: 1464 No. Test Specimen Volume Special Precautions Turnaround Time Reference Ranges** INAB Accredited Test Reg No. 208MT Reticulocyte count (Adult) 2.5ml K3EDTA (Baby) 1.3ml K3EDTA Also requires a FBC result. Routine: 24 Urgent- 20 minutes Automated Adults: *** Pregnant:50-150x 109/L Non-pregnant:35.2 – 122.8 x 109/L Babies: Automated < 1 Day: 110-450x 109/L 2-7 Days: 10-80x109/L 7 Dys-1 Months: 10-80x109/L Fibrinogen (Adult) (Baby) 1 part 3.2% Na Citrate 9 parts blood Adult vol.= 3 ml Baby vol.= 1.3 ml Interference: Heparin >1μ.ml, Hb> 150 mg/dL Trigs >1022 mg/dL, Billi> 19.6 mg/dL Test plasma within 4 hours of specimen collection. Routine - 2 hr Urgent –30mins Adults: Normal: 1.5-4.0 g/L Pregnant: 2.0- 6.0 g/L Babies(Term****): 1 Day: 2.25-3.41 g/L 1-5 Days: 2.37-3.87 g/L Activated Partial Thromboplastin Time (APTT) 1 part 3.2% Na Citrate 9 part blood Adult vol.= 3 ml Baby vol. = 1.3 ml Inform lab if patient is on anticoagulant therapy. Test plasma within 4 hours of specimen collection. Haemolysed samples should not be processed. Volume of blood to citrate critical Take samples from patients on heparin drips above the drip or opposite arm. Routine - 2 hr Urgent Adults: 25.3 – 36.3 sec Babies (Term****): Day 1: 37.1-48.74 sec Day 5: 33.9-51.2 sec Day 30: 32.3-47.8 sec Day 90: 30.6-43.67 sec
International normalised ratio (Adult only for patients on warfarin (INR) 1 part 3.2% Na Citrate 9 parts blood Test plasma within 4 hours of collection. ISI is derived from manufacturer’s instructions. Interference: Heparin>2 U/dl Haemolysed Specimens should not be processed. Volume of blood to anticoagulant critical. Routine - 2 hr Urgent –30mins Therapeutic Range as per BSCH guidelines. Target ranges available corresponding to clinical conditions Prothrombin Time (PT) 1 part 3.2% Na Citrate 9 part blood Adult vol.= 3mls Baby vol = 1.3ml Test within 4 hours of specimen collection. Notify lab if patient is on anticoagulant therapy. Interferences: Haemolysed samples, Lipaemic samples Routine - 2 hr Urgent –30mins Adults: 9.6-12.3s Babies (Term****): Day 1: -11.6-14.4-sec Day 5: -10.9-13.9 sec Day 30:-10.6-13sec Day 90:-11.5-13.1 sec Lupus Anticoagulant (LA) Adult Only 1 part 3.2% Na Citrate 9 part blood Adult vol.= 6 ml (2 x 3 mls) Complete Thrombophilia request form IUD bloods for lupus must be sent at least 8 weeks post IUD. Send sample as soon as possible, within one hour to allow processing and freezing within 4 hours. Do not send to lab after 15.30pm Stored frozen at –80C 4-6 weeks (Batched) Reported as Positive or negative. Positives must be confirmed by repeat testing at least 12 weeks apart to confirm positively.
event and preferably within 24 hrs. Additional Anti-D must be administered within 72 hours of sensitising event if required. State clinical details, gestation and rhesus status on request form. Routine – 1-2 Days Urgent – Same Day Anti-D/12ml bleed If >8mL in Rh D negative patient maybe referred for flow Full Blood count with differential and /or film review Consultant Blood Film review Adult 2.5ml K3EDTA Baby: 1.3ml K3EDTA Adult 2.5ml K3EDTA Baby: Analyse within 24 hours if possible. Store at 4C if testing is delayed Make film within 8 hours Make film within 8 hours NB Film review must be accompanied Mon-Fri 3 pm Routine Outpatients - 24 hours Inpatients: 2-4 hours Urgent – 30 min’s To include manual differential – 40 min’s (For NICU HB and HCT results will be available provisionally on LIMS and Powerchart ). Consultant Blood film review: WCC* RCC* Hb* Plts* HCT* MCV* MCH* MCHC* RDW* Neut* Lymph* Mono*, Eos*