• Phytoplankton, pigments, picoplankton, Suspended solids – “Integrated Water Column” sample: • Measure and Record the residual volume left after the chemistry samplings in the surface niskin bottle, because this is the only Carbon sampling depth common to all sites. • Base the collection of the remaining residual volumes, to be added to the 20 litre “integrated” carboy sample, on the measured residual volume from the surface niskin bottle to avoid a volume bias from a particular depth. Use the measuring jugs and funnels made from “native/natural“ plastic for this • Discard from niskins sampled deeper than the surface bottle, anything in excess of the volume equal to that obtained from the full sampling conducted from the surface niskin. • Do not include water sampled from deeper than 50m in the carboy. • Phytoplankton pigments – samples from depths equating to moored WQM sensor packs: • There is a requirement for another cast where a niskin of water is collected from each of the 2 depths that correlate with the moored WQM sensors, for pigment analysis (by HPLC) to check WQM pigment performance • As with the water collected for the “Water Column” carboy sample, use the funnels made from “native/natural“ plastic to collect the niskin contents into individual, labelled, 5 litre containers. Genomic surface sampling for microbial and phytoplankton analyses requires a 3 litre niskin sample collection from the same depths • Remember to keep all collected waters cold/cool and in the shade to minimise sample degradation. • Keep collected waters in carboys and containers quite separate from other samples which may hold any formalin whatsoever • Where and when necessary, preserve or pre-treat samples as outlined in the detailed sampling protocols for each parameter as described below • Endeavour to avoid sample biodegradation by holding these samples from collection time to post-processing, to less than four (4) hours maximum. IMOS National Reference Station Field Sampling BIOGEOCHEMICAL OPERATIONS MANUAL For discrete water samples to be taken, 5L Niskin bottles triggered by messenger (Brass
Shipping samples preserved in liquid nitrogen Follow details of preparing the dry shipping Dewar (above) When the dry shipper is prepared as above, remove the following samples from the station storage Dewar and place in the dry shipper: • cryovials of screened sample from the black jar for zooplankton molecular analysis • cryovials of HPLC phytoplankton (pigment) WC samples • cryovials from the WQM depths for HPLC (pigment) analysis • cryovials for flow cytometry It’s very helpful if you pack each type of sample separately, held on cryo-canes or cryo-sleeves (plastic tubes) or tied in batches inside “knee-hi” stockings. Package and consignment note must carry the wording: “NOT RESTRICTED as per IATA SPECIAL PROVISION A152” Please dispatch the dry shipper, preferably by TOLL PRIORITY OVERNIGHT, or TNT Overnight or Australian Air Express (please do not use TOLL-IPEC Priority) to: Samples arriving Monday – Tuesday: Samples arriving Wed – Friday: Attention: Ros Watson Attention: Lesley Clementson CSIRO Marine & Atmospheric Research CSIRO Marine & Atmospheric Research Castray Esplanade Castray Esplanade Hobart TAS 7000 Hobart TAS 7000 Ph: (03) 62325268 or (03) 62325347 Ph: (03) 62325337 or (03) 62325347 M: 0400567141 M: 0409140230 Email: If you can’t reach one of the above, try other numbers and copy emails. • Remember, these liquid nitrogen stored samples should always be shipped in dry shipper Dewars – not on dry ice – as they will otherwise rapidly degrade. TSS samples should also be shipped to Ros Watson; for convenience they can be packed in batches and sent inside the travel case of the dry shipper (not frozen). Or they may be sent with the salinity crates as previously. IMOS National Reference Station Field Sampling Storage and shipping of all samples from a sampling trip Transport of NRS samples to point of analyses: Unless noted elsewhere in the transport requirements, the samples can be transferred quarterly.
• In DOS type: dir/w <Enter> cd “SYYCC” <Enter> dir/w <Enter> move ..\”name of last run” . <Enter> move “name of last run” “name of last run”.scp (check this has been done by typing “dir/w” <Enter>) Insert a 3.5” floppy disk into “A” drive Type – copy “name of last run”.scp A: The dissolved oxygen calculated is expressed as µML-1 Are there alternate SI units to report these parameters in? IMOS National Reference Station Field Sampling BIOGEOCHEMICAL OPERATIONS MANUAL HYDROCHEMISTRY ANALYSES
• Once the filters have reached constant weight store in the appropriately numbered Millipore Petri-slides until required. On TSM log sheet record the number of the Petri-slide along with the weight of the filter stored in the Petri-slide. • Always do the initial and final post-sampling weighing of the filters on the same balance. Suspended Matter (SM) Analysis (total, organic and inorganic) • After the NRS Samplers have collected the Suspended Matter sample in the field: • Place filters in glass petri dishes, each labelled with the same number as that on the petri slide from which each filter came. Each petri dish will contain 3 small balls of aluminium foil on which the filter will sit . • Place petri dishes on a tray (cake tin), cover with a sheet of aluminium foil and place in an oven at 75o C for approximately 3 hours. • Remove from oven and let cool (around 15 minutes). • Weigh each filter, record weight on the TSM log sheet against the same number and return the filter to the same petri dish. • Return petri dishes to the oven at 75o C for approximately another 1-2 hours. • Remove, cool and weigh again. • Generally after 2 weighings, the filters should have reached constant weight. If there is more than 0.2 mg difference between the first and second weighing, repeat the drying/weighing process. • Determine the TSM weight by subtraction of the pre- filtration weight from the post-filtration weight. • Take note of the sample volume that was filtered through the filter. • Calculate the weight per volume (Total). • Return filters to glass petri dishes and place petri dishes on the floor of a muffle furnace (note the position of each of the numbered dishes as the numbering on the dishes will be removed during the muffling process). Cover the dishes loosely with a sheet of aluminium foil and program the muffle furnace to 450o C. After the furnace has reached this temperature, wait 3 hours before programming the temperature of the furnace to 20o C. When the furnace has reached 20o C, remove the dishes and filters and weigh immediately.
• At the start of the day ensure all solutions and frozen samples reach ambient laboratory temperature. • Prepare fresh reagents for the analytes that are to be tested for the day as well as fresh standards and intermediate standards. • Flush the system for 15 minutes with carrier solution water and reagents - ensure that the cadmium column is not switched in, • The carrier solution is matched as closely as possible to the sample salinity – i.e. Artificial Sea Water (ASW)or pure reagent grade water (PW) • Use this time to complete preparation of fresh reagents. • After 2 minutes turn the cadmium column in to refresh the buffer, or make a fresh cadmium column. • The chemistries which are used by the Hobart hydrochemistry group, are methods developed by Lachat and run directly as outlined in the following list: QuickChem Method 31-115-01-1-I Determination of Orthophosphate by Flow Injection Analysis QuickChem Method 31-107-04-1-A Determination of Nitrate in brackish or seawater by Flow Injection Analysis QuickChem Method 31-114-27-1-D Determination of Silicate by Flow Injection Analysis QuickChem Method 31-107-06-4-A Determination of Ammonia in Brackish or Seawater by flow injection analysis (To be increased in sensitivity by further CMAR modifications) • Once the system is stable – usually only 15 minutes or so, the run can be started. • At the end of the day/run the system is flushed for about 15 minutes on reagent grade water, and then shut down.
Depth: ALK DIC Salinity # DOX# Carboy Vol. Niskin #: Nutrients # Depth: ALK DIC Salinity # DOX# Carboy Vol. Niskin #: Nutrients # Depth: ALK DIC Salinity # DOX# Carboy Vol. Niskin #: Nutrients # NISKIN BOTTLES – SECOND CAST Start/Finish Time / Depth: ALK DIC Salinity # DOX# Carboy Vol. Niskin #: Nutrients # Depth: ALK DIC Salinity # DOX# Carboy Vol. Niskin #: Nutrients # Depth: ALK DIC Salinity # DOX# Carboy Vol. Niskin #: Nutrients # Depth: ALK DIC Salinity # DOX# Carboy Vol. Niskin # Nutrients # Depth: ALK DIC Salinity # DOX# Carboy Vol. Niskin #: Nutrients # NISKIN BOTTLE- CAST FOR WQM PIGMENT CALIBRATION Upper Depth Niskin # Time Collected Lower Depth Niskin # Time Collected