MANAGER I consider that the research activity detailed in this application is justified on scientific and/or educational grounds. I confirm that appropriate facilities are available to ensure the welfare of the animals over the course of the project. I am satisfied that all personnel who will be involved with the proposed investigation are familiar with and will comply with all relevant Commonwealth legislation, the Tasmanian Animal Welfare Act 1993 and the Australian code for the care and use of animals for scientific purposes, 8th edition, 2013 (the Code). I shall ensure, as far as I am able, that the principles of the Code will be complied with throughout the investigation. Name: Email: Position in organisation: Signature: Date: CHIEF (AND ALTERNATIVE CHIEF) INVESTIGATOR I confirm that I have the appropriate qualifications and experience to perform the procedures described in this application and will ensure the procedures are conducted in accordance with any conditions of AEC approval. I am familiar and will comply with all relevant Commonwealth legislation, the Tasmanian Animal Welfare Act 1993 and the Australian code for the care and use of animals for scientific purposes, 8th edition, 2013 (the Code). I accept responsibility for the ethical conduct of the proposed investigation, according to the principles outlined in the Code. I acknowledge that the care and maintenance of animals for scientific or educational purposes is the ultimate responsibility of the Chief Investigator, but that this work may be delegated to suitably trained and qualified associate investigators that are approved by the AEC to work on this investigation. I undertake not to use any animals or carry out any procedures which have not been approved by the AEC. I undertake to advise the AEC of any modifications to my project from what is presented in this application prior to those amendments being implemented. I undertake to comply with all relevant AEC policies and requirements, including the reporting of any unexpected adverse events. Name of Chief Investigator: Signature: Date: Name of Alternative Chief Investigator: Signature: Date:
Anaesthesia Fasting Induction - drug, dose, route Maintenance - drug, dose, route Methods of monitoring anaesthesia and recovery Additional support during anaesthesia and recovery (eg heat, intravenous fluids) Location of induction and recovery areas Restraint Expertise of personnel Blood/body fluid collection Volume Route Frequency Anaesthesia or analgesia Restraint Animal monitoring (methods, frequency) Diet/water modifications Type Amount Effects Measurement of intake Animal monitoring Drug treatments Substance Volume/dose Route Frequency/total number per animals Local and systemic effects Anaesthesia or analgesia Possible side effects Restraint Expertise of personnel Euthanasia Method Location (where procedure will be performed) Expertise of personnel Genetic manipulation Methods Potential effects In-vitro studies Source of animals Duration held Euthanasia Teaching Housing Duration held Method of disposal Surgery Anaesthesia Location of pre-operative preparation area Pre-operative preparation Surgical procedure (site, technique) Sterile technique (instruments, drapes, surgeon) Location of and housing in post-operative recovery area Post-operative management Post-operative monitoring (methods, frequency, duration) Use of analgesics (type, dose, route, frequency, means of determining necessity for use) Expertise of personnel Tumour/neoplasia induction Method Site Endpoint Animal monitoring (methods, frequency) Toxicology Substance Volume/dose Route Frequency of treatment/total no per animals Local and systemic effects Anaesthesia or analgesia Restraint Animal monitoring (methods, frequency) Endpoint/duration Wildlife studies Location Methods Capture methods Handling/restraint Housing Monitoring Release Effects on population Transport Type Duration Confinement Numbers of animals Air-conditioning Housing Location Isolation Group housing (stocking rates, sexes) Shelter Bedding Hiding areas Environmental enrichment Duration held Conditioning period