Antimicrobial susceptibility testing of Escherichia coli, enterococci and staphylococci TEST FORMS Name: Name of laboratory: Name of institute: City: Country: E-mail: Fax: Comments: TEST FORMS METHODS - Enterococci Which method did you use for antimicrobial susceptibility testing of enterococci in this EQAS: MIC – Broth microdilution MIC – Agar dilution (note: not evaluated in the final report) Brand: How many Enterococcus spp. isolates does your laboratory annually isolate: How many Enterococcus spp. isolates does your laboratory annually test for antimicrobial susceptibility by a MIC method: Which method was followed for the preparation of the inoculum? Please describe: Which standard was followed (TREK, CLSI…)
Enterococci EURL ENT. 12.1 E. faecium E. faecalis Ampicillin AMP Chloramphenicol, CHL Ciprofloxacin, CIP Daptomycin, DAP Erythromycin, ERY Gentamicin, GEN Linezolid, LZD Quin.-Dalf. (Synercid), SYN Teicoplanin, TEI Tetracycline, TET Tigecycline, TGC Vancomycin, VAN Strain Antimicrobial Results and interpretation > MIC-value (μg/ml) S / R Enterococci EURL ENT. 12.2 E. faecium E. faecalis Ampicillin AMP Chloramphenicol, CHL Ciprofloxacin, CIP Daptomycin, DAP Erythromycin, ERY Gentamicin, GEN Linezolid, LZD Quin.-Dalf. (Synercid), SYN Teicoplanin, TEI Tetracycline, TET Tigecycline, TGC Vancomycin, VAN TEST FORM - Enterococci Strain Antimicrobial Results and interpretation > MIC-value (μg/ml) S / R Enterococci Ampicillin AMP
EURL ENT. 12.3 E. faecium E. faecalis Chloramphenicol, CHL Ciprofloxacin, CIP Daptomycin, DAP Erythromycin, ERY Gentamicin, GEN Linezolid, LZD Quin.-Dalf. (Synercid), SYN Teicoplanin, TEI Tetracycline, TET Tigecycline, TGC Vancomycin, VAN Strain Antimicrobial Results and interpretation > MIC-value (μg/ml) S / R Enterococci EURL ENT. 12.4 E. faecium E. faecalis Ampicillin AMP Chloramphenicol, CHL Ciprofloxacin, CIP Daptomycin, DAP Erythromycin, ERY Gentamicin, GEN Linezolid, LZD Quin.-Dalf. (Synercid), SYN Teicoplanin, TEI Tetracycline, TET Tigecycline, TGC Vancomycin, VAN TEST FORM - Enterococci Strain Antimicrobial Results and interpretation > MIC-value (μg/ml) S / R Enterococci Ampicillin AMP Chloramphenicol, CHL
EURL ENT. 12.5 E. faecium E. faecalis Ciprofloxacin, CIP Daptomycin, DAP Erythromycin, ERY Gentamicin, GEN Linezolid, LZD Quin.-Dalf. (Synercid), SYN Teicoplanin, TEI Tetracycline, TET Tigecycline, TGC Vancomycin, VAN Strain Antimicrobial Results and interpretation > MIC-value (μg/ml) S / R Enterococci EURL ENT. 12.6 E. faecium E. faecalis Ampicillin AMP Chloramphenicol, CHL Ciprofloxacin, CIP Daptomycin, DAP Erythromycin, ERY Gentamicin, GEN Linezolid, LZD Quin.-Dalf. (Synercid), SYN Teicoplanin, TEI Tetracycline, TET Tigecycline, TGC Vancomycin, VAN TEST FORM - Enterococci Strain Antimicrobial Results and interpretation > MIC-value (μg/ml) S / R Enterococci EURL ENT. Ampicillin AMP Chloramphenicol, CHL Ciprofloxacin, CIP
Chloramphenicol, CHL Ciprofloxacin, CIP Daptomycin, DAP Erythromycin, ERY Gentamicin, GEN Linezolid, LZD Quinupristin-Dalfopristin (Synercid), SYN Teicoplanin, TEI Tetracycline, TET Tigecycline, TIG Vancomycin, VAN TEST FORMS - Staphylococci Strain Antimicrobial Results and interpretation > MIC-value (μg/ml) S / R S. aureus EURL ST 12.1 Cefoxitin, FOX Chloramphenicol, CHL Ciprofloxacin, CIP Clindamycin, CLN Erythromycin, ERY Fusidic acid, FUS Gentamicin, GEN Kanamycin, KAN Linezolid, LZD Mupirocin, MUP Penicillin, PEN Quin.-Dalf. (Synercid), SYN Rifampicin, RIF Streptomycin, STR Sulfamethoxazole, SMX
TEST FORMS - Staphylococci Strain Antimicrobial Results and interpretation > MIC-value (μg/ml) S / R S. aureus EURL ST 12.6 Cefoxitin, FOX Chloramphenicol, CHL Ciprofloxacin, CIP Clindamycin, CLN Erythromycin, ERY Fusidic acid, FUS Gentamicin, GEN Kanamycin, KAN Linezolid, LZD Mupirocin, MUP Penicillin, PEN Quin.-Dalf. (Synercid), SYN Rifampicin, RIF Streptomycin, STR Sulfamethoxazole, SMX Tetracycline, TET Tiamulin (TIA) Trimethoprim, TMP Vancomycin, VAN Methicillin resistance (MRSA) Positive Negative
Presumptive ESBL Presumptive ESBL+ AmpC Presumptive AmpC Presumptive carbapenemase Other phenotype Susceptible Comments (include optional genotype or other results): TEST FORM – E. coli Strain Antimicrobial Results and interpretation > MIC-value (μg/ml) S / R E. coli EURL EC 12.5 Ampicillin, AMP Azithromycin, AZT Cefotaxime, FOT Ceftazidime, TAZ Chloramphenicol, CHL Ciprofloxacin CIP Colistin, COL Gentamicin, GEN Meropenem, MERO Nalidixic acid, NAL Sulfamethoxazole, SMX Tetracycline, TET Tigecycline, TGC Trimethoprim, TMP All strains resistant to cefotaxime (FOT), ceftazidime (TAZ) and/or meropenem (MERO) should be tested in the second panel for confirmatory tests for ESBL/AmpC/carbapenemase production. See further description of confirmatory tests in the protocol section ‘3.3.1 E. coli’. Strain Antimicrobial Results and interpretation > MIC-value (μg/ml) S / R E. coli EURL EC 12.5 Cefepime, FEP Cefotaxime, FOT Cefotaxime + clavulanic acid (F/C) Cefoxitin, FOX Ceftazidime, TAZ Ceftazidime+ clavulanic acid (T/C) Ertapenem, ETP Imipenem, IMI Meropenem, MERO Temocillin, TRM Interpretation of PANEL 2 results:
Interpretation of PANEL 2 results: Presumptive ESBL Presumptive ESBL+ AmpC Presumptive AmpC Presumptive carbapenemase Other phenotype Susceptible Comments (include optional genotype or other results): TEST FORM – E. coli Strain Antimicrobial Results and interpretation > MIC-value (μg/ml) S / R E. coli EURL EC 12.8 Ampicillin, AMP Azithromycin, AZT Cefotaxime, FOT Ceftazidime, TAZ Chloramphenicol, CHL Ciprofloxacin CIP Colistin, COL Gentamicin, GEN Meropenem, MERO Nalidixic acid, NAL Sulfamethoxazole, SMX Tetracycline, TET Tigecycline, TGC Trimethoprim, TMP All strains resistant to cefotaxime (FOT), ceftazidime (TAZ) and/or meropenem (MERO) should be tested in the second panel for confirmatory tests for ESBL/AmpC/carbapenemase production. See further description of confirmatory tests in the protocol section ‘3.3.1 E. coli’. Strain Antimicrobial Results and interpretation > MIC-value (μg/ml) S / R E. coli EURL EC 12.8 Cefepime, FEP Cefotaxime, FOT Cefotaxime + clavulanic acid (F/C) Cefoxitin, FOX Ceftazidime, TAZ Ceftazidime+ clavulanic acid (T/C) Ertapenem, ETP Imipenem, IMI Meropenem, MERO Temocillin, TRM