Which solvent was used for the preparation of the 0.5 McFarland solution (water, saline) Please describe in detail how you prepared the dilution of the inoculum (including the volume in final MH-dilution and intended dilution level; e.g. diluted 1:1000 by adding 10µl of 0.5 McFarland solution in 10ml MH broth, for an expected inoculum of 1*105 CFU/ml) Comments or additional information: TEST FORM Does your laboratory have an accreditation for Campylobacter AST? Yes No Incubation conditions: 36-37ºC / 48h 42ºC / 24h Method used for antimicrobial susceptibility testing of Campylobacter in this EQAS: Broth microdilution Agardilution Brand of microbroth plates/agar: How many Campylobacter isolates does your laboratory annually isolate: How many Campylobacter isolates does your laboratory annually susceptibility test: Which method was followed for the preparation of the inoculum (please describe) Which standard was followed (TREK, CLSI…) Which solvent was used for the preparation of the 0.5 McFarland solution (water, saline)
C. coli Nalidixic acid Streptomycin Tetracycline TEST FORM Strain Antimicrobial Interpretation MIC-value (μg/ml) S / R Campylobacter EURL C-12.5 C. jejuni C. coli Ciprofloxacin Erythromycin Gentamicin Nalidixic acid Streptomycin Tetracycline Campylobacter EURL C-12.6 C. jejuni C. coli Ciprofloxacin Erythromycin Gentamicin Nalidixic acid Streptomycin Tetracycline Campylobacter EURL C-12.7 C. jejuni C. coli Ciprofloxacin Erythromycin Gentamicin Nalidixic acid Streptomycin Tetracycline Campylobacter EURL C-12.8 C. jejuni Ciprofloxacin Erythromycin Gentamicin Nalidixic acid
C. coli Streptomycin Tetracycline TEST FORM Susceptibility testing of Campylobacter jejuni reference strain ATCC 33560 Strain Antimicrobial MIC-value (μg/ml) 36 °C/48 hours 42 °C/24 hours C. jejuni ATCC 33560 Ciprofloxacin Erythromycin Nalidixic acid Tetracycline For Agar dilution: Susceptibility testing of Campylobacter jejuni reference strain ATCC 33560 Strain Antimicrobial MIC-value (μg/ml) C. jejuni ATCC 33560 Ciprofloxacin Erythromycin Gentamicin Nalidixic acid Tetracycline
TEST FORM – genotypic characterisation Genotypic characterisation of the test strains Strain code: Method used: If PCR-methods, additional information should be given below Gene: Found Tested, not found Published method , reference: In-house method Primer used 5’→3’: Primer used 3’→5’: Gene: Found Tested, not found Published method , reference: In-house method Primer used 5’→3’: Primer used 3’→5’: Gene: Found Tested, not found Published method , reference: In-house method Primer used 5’→3’: Primer used 3’→5’: Gene: Found Tested, not found Published method , reference: In-house method Primer used 5’→3’: Primer used 3’→5’: Gene: Found Tested, not found Published method , reference: In-house method Primer used 5’→3’: Primer used 3’→5’: Gene: Found Published method , reference: In-house method Primer used 5’→3’:
Tested, not found Primer used 3’→5’: Strain code: Method used: If PCR-methods, additional information should be given below Gene: Found Tested, not found Published method , reference: In-house method Primer used 5’→3’: Primer used 3’→5’: Gene: Found Tested, not found Published method , reference: In-house method Primer used 5’→3’: Primer used 3’→5’: Gene: Found Tested, not found Published method , reference: In-house method Primer used 5’→3’: Primer used 3’→5’: Gene: Found Tested, not found Published method , reference: In-house method Primer used 5’→3’: Primer used 3’→5’: Gene: Found Tested, not found Published method , reference: In-house method Primer used 5’→3’: Primer used 3’→5’: Comments: