Supplementary Information S9. Phyla or Division Class Species Life stage Duration Test type Measure (Endpoint) Test medium Salinity (‰) Temp. (C) pH Concentration (µg/L) Reference Arthropoda Malacostraca Mud Crab (Neopanope texana) 15 mm 4 d Acute LC50 (Mortality) * * * * 1 000 000 U.S. EPA (2015) 1 000 000 GEOMETRIC MEAN# Malacostraca Pink Shrimp (Penaeus duorarum) 55 mm 4 d Acute LC50 (Mortality) Natural or artificial seawater 20 ± 3 23 ± 1 - 11 300 U.S. EPA (2015) 11 300 GEOMETRIC MEAN# Malacostraca Shrimp (Palaemonetes kadiakensis) - 2 d Acute LC50 (Mortality) Natural or artificial seawater 20 ± 3 23 ± 1 - 10 000 U.S. EPA (2015) 10 000 GEOMETRIC MEAN# Entognatha Springtail (Proisotoma minuta) 60-day old 7 d Chronic LC50 (Mortality) Salt solution (synthetic sea salt in nanopure water) - 23 ± 1 5.7 >200 000 Park and Lees (2005) >200 000 GEOMETRIC MEAN@ Bacillariophyta Fragilariophyceae Diatom (Ceratoneis closterium) Exponential growth 3 d Chronic IC10 (Growth rate) Filtered (0.45 μm) seawater 35 ± 2 21 ± 2 8.2 ± 0.1 310 Hook and Osborn (2014) 310 GEOMETRIC MEAN 310 VALUE USED IN SSD Fragilariophyceae Diatom (Ceratoneis closterium) Exponential growth phase 3 d Chronic IC50 (Growth rate) Filtered (0.45 μm) seawater 35 ± 2 21 ± 2 8.2 ± 0.1 >1000 Hook and Osborn (2014)
Actinopterygii Gilt-head bream (Sparus aurata) 3h – 5h post-hatch larvae 3 d Acute LC50 (Mortality) Natural and filtered (0.45 μm) sea water 37 ± 1 19 ± 1 8 ± 0.1 4190 Arufe et al. (2004) 4190 GEOMETRIC MEAN# Actinopterygii Gilt-head bream (Sparus aurata) 3h – 5h post-hatch larvae 3 d Acute NOEC (Mortality) Natural and filtered (0.45 μm) sea water 37 ± 1 19 ± 1 8 ± 0.1 2250 Arufe et al. (2004) 2250 GEOMETRIC MEAN$ Actinopterygii Gilt-head (Sparus aurata) 3h – 5h post-hatch larvae 3 d Acute LOEC (Mortality) Natural and filtered (0.45 μm) sea water 37 ± 1 19 ± 1 8 ± 0.1 4500 Arufe et al. (2004) 4500 GEOMETRIC MEAN^ Actinopterygii Gilt-head bream (Sparus aurata) 3h – 5h larvae 3 d Acute NOEC (Dry weight) Natural and filtered (0.45 μm) sea water 37 ± 1 19 ± 1 8 ± 0.1 4500 Arufe et al. (2004) Actinopterygii Gilt-head bream (Sparus aurata) 3h – 5h post-hatch larvae 3 d Acute NOEC (Dry weight) Natural and filtered (0.45 μm) sea 37 ± 1 19 ± 1 8 ± 0.1 >4500 Arufe et al. (2004) 4500 GEOMETRIC MEAN$ Actinopterygii Sheepshead Minnow (Cyprinodon variegatus) 0.36 g 4 d Acute LC50 (Mortality) Seawater 20 ± 5 22 ± 2 >7.5 and <8.5 430 U.S. EPA (2015) 430 GEOMETRIC MEAN# Actinopterygii Striped Bass (Morone saxatilis) 63 mm 4 d Acute LC50 (Mortality) Seawater 20 ± 5 - >7.5 and <8.5 300 U.S. EPA (2015) 300 GEOMETRIC MEAN#
Haptophyta Prymnesiophyceae Microalgae (Isochrysis galbana) - 10 d Chronic EC50 (Cell density) Algal nutrient medium 30 ± 5 20 – 24 ± 2 Same as media 100 U.S. EPA (2015) 100 GEOMETRIC MEAN@ 100 VALUE USED IN SSD Mollusca Bivalvia Eastern Oyster (Crassostrea virginica) 16–29g 7 d EC50 (Mortality) Natural or artificial seawater >12 ± 2 (similar to 20 ± 5 - 200 U.S. EPA (2015) 200 GEOMETRIC MEAN# Bivalvia Eastern Oyster (Crassostrea virginica) SPAT 4 d Acute EC50 (Mortality) Natural or artificial seawater >12 ± 2 (similar to 20 ± 5 - 370 U.S. EPA (2015) 370 GEOMETRIC MEAN# Ochrophyta Diatom (Skeletonema costatum) - 5 d Chronic EC50 (Cell density) Algal nutrient medium 30 ± 5 20 – 24 ± 2 Same as media 120 U.S. EPA (2015) 120 GEOMETRIC MEAN@ 120 USED IN SSD * Conducted using USEPA methods – exact values cannot be located. # Values were acute LC/EC50 data that were converted to chronic NOEC/EC10 values by dividing by 10 (Warne, 2001). $ Values were acute NOEC data that were converted to chronic NOEC/EC10 values by dividing by 2 (Warne, 2001). @ Values were chronic EC/LC50 values that were converted to chronic NOEC/EC10 values by dividing by 5 (Warne, 2001). & Values were chronic LOEC values that were converted to chronic NOEC/EC10 values by dividing by 2.5 (Warne, 2001). % Values were acute LC/EC10 data that were converted to chronic NOEC/EC10 values by dividing by 2 (Warne, 2001). ^ Values were acute LOEC that were converted to chronic NOEC/EC10 values by dividing by 5 (Warne, 2001).