dechlorinated tap water 113 GEOMETRIC MEAN Arthropoda Branchiopoda Freshwater Flea (Daphnia magna) Life Cycle 21 d Chronic NOEL (Body length/Dry weight) Surface or ground, reconstituted or dechlorinated tap 20 ± 1 - 57 US EPA (2015a) 57 GEOMETRIC MEAN Arthropoda Branchiopoda Freshwater Flea (Daphnia magna) Life Cycle 28 d Chronic LOEC (Body length/Dry weight) Surface or ground, reconstituted or dechlorinated tap 20 ± 1 - 200 US EPA (2015a) 200 GEOMETRIC MEAN Arthropoda Branchiopoda Freshwater Flea (Daphnia magna) Life Cycle 28 d Chronic NOEL (Body length/Dry weight) Surface or ground, reconstituted or dechlorinated tap water 20 ± 1 - 200 US EPA (2015a) 200 GEOMETRIC MEAN Arthropoda Branchiopoda Freshwater Flea (Daphnia pulex) 1st Instar larvae 2 d EC50 (Body length/Dry weight) Surface or ground, reconstituted or dechlorinated tap 20 ± 2 - 1400 US EPA 1400 GEOMETRIC MEAN Arthropoda Branchiopoda Freshwater Flea (Daphnia - 2 d Acute EC50 (Immob) Reconstituted de-ionised water 18 6.5–8.5 1400 Sanders and Cope (1966) Arthropoda Branchiopoda Freshwater Flea (Daphnia pulex) - 2 d Acute EC50 (Immob) Well water, reconstitued water 15 ± 1 7.4–7.8 1400 Johnson and Finley (1980) 1400 GEOMETRIC MEAN Arthropoda Branchiopoda Freshwater Flea (Daphnia pulex) Adults (5 days old) 4 d Acute LC50 (Mortality) 0.45 um filtered well water - 6.9 ± 0.1 17900 Nebeker and Schuytema (1998) 17900 GEOMETRIC MEAN Arthropoda Branchiopoda Freshwater Flea (Daphnia pulex) Adults (5 days old) 7 d Chronic NOAEL (Mortality) 0.45 um filtered well - 6.9 ± 0.1 4000 Nebeker and Schuytema (1998) 4000 GEOMETRIC MEAN
39 GEOMETRIC MEAN 39@ VALUE USED IN SSD Bacillariophyta Bacillariophyceae Microalgae (Encyonema silesiacum) Exponential Growth Phase 4 d Chronic EC05 (Cell density) DV culture medium 21 ± 2 - 3.11 Larras et al. (2012) 3.11 GEOMETRIC MEAN 3.11 USED IN SSD Bacillariophyta Bacillariophyceae Microalgae (Encyonema silesiacum) - 4 d Chronic EC10 (Growth fluorescence) DV culture medium - - 90 Larras et al. (2013) Bacillariophyta Bacillariophyceae Microalgae (Encyonema silesiacum) Exponential Growth Phase 4 d Chronic EC10 (Growth fluorescence) DV culture medium - - 3.98 Larras et al. (2013) 18.93 GEOMETRIC MEAN Bacillariophyta Bacillariophyceae Microalgae (Encyonema silesiacum) Exponential Growth Phase 4 d Chronic EC50 (Cell density) DV culture medium 21 ± 2 - 8.79 Larras et al. (2012) 8.79 GEOMETRIC MEAN Bacillariophyta Bacillariophyceae Microalgae (Encyonema silesiacum) - 4 d Chronic EC50 (Growth fluorescence) DV culture medium - - 286 Larras et al. (2013) 286 GEOMETRIC MEAN Bacillariophyta Bacillariophyceae Microalgae (Eolimna minima) Exponential Growth Phase 4 d Chronic EC05 (Cell density) DV culture medium 21 ± 2 - 3007 Larras et al. (2012) 3007 GEOMETRIC MEAN 3007 USED IN SSD Bacillariophyta Bacillariophyceae Microalgae (Eolimna minima) Exponential Growth Phase 4 d Chronic EC50 (Cell density) DV culture medium 21 ± 2 - 4236 Larras et al. (2012) 4236 GEOMETRIC MEAN Bacillariophyta Fragilariophyceae Microalgae (Fragilaria capucina var vaucheriae) Exponential Growth Phase 4 d Chronic EC05 (Cell density) DV culture medium 21 ± 2 - 0.069 Larras et al. (2012)
0.069 GEOMETRIC MEAN 0.069 VALUE USED IN SSD Bacillariophyta Fragilariophyceae Microalgae (Fragilaria capucina var vaucheriae) - 4 d Chronic EC10 (Growth fluorescence) DV culture medium - - 21 Larras et al. (2013) Bacillariophyta Fragilariophyceae Microalgae (Fragilaria capucina var vaucheriae) Exponential Growth Phase 4 d Chronic EC10 (Growth fluorescence) DV culture medium - - 0.11 Larras et al. (2013) 1.52 GEOMETRIC MEAN Bacillariophyta Fragilariophyceae Microalgae (Fragilaria capucina var vaucheriae) Exponential Growth Phase 4 d Chronic EC50 (Cell density) DV culture medium 21 ± 2 - 4.03 Larras et al. (2012) 4.03 GEOMETRIC MEAN Bacillariophyta Fragilariophyceae Microalgae (Fragilaria capucina var vaucheriae) - 4 d Chronic EC50 (Growth fluorescence) DV culture medium - - 44 Larras et al. (2013) 44 GEOMETRIC MEAN Bacillariophyta Fragilariophyceae Microalgae (Fragilaria rumpens) Exponential Growth Phase 4 d Chronic EC05 (Cell density) DV culture medium 21 ± 2 - 18 Larras et al. (2012) 18 GEOMETRIC MEAN Bacillariophyta Fragilariophyceae Microalgae (Fragilaria rumpens) - 4 d Chronic EC10 (Growth fluorescence) DV culture medium - - 0.76 Larras et al. (2013) Bacillariophyta Fragilariophyceae Microalgae (Fragilaria rumpens) Exponential Growth 4 d Chronic EC10 (Growth fluorescence) DV culture medium - - 30 Larras et al. (2013) 4.77 GEOMETRIC MEAN 4.77 VALUE USED IN SSD Bacillariophyta Fragilariophyceae Microalgae (Fragilaria rumpens) Exponential Growth Phase 4 d Chronic EC50 (Cell density) DV culture medium 21 ± 2 - 122 Larras et al. (2012) 122 GEOMETRIC MEAN
232.05 VALUE USED IN SSD Bacillariophyta Bacillariophyceae Microalgae (Gomphonema parvulum) Exponential Growth 4 d Chronic EC50 (Cell density) DV culture medium 21 ± 2 - 2255 Larras et al. (2012) 2255 GEOMETRIC MEAN Bacillariophyta Bacillariophyceae Microalgae (Gomphonema parvulum) - 4 d Chronic EC50 (Growth fluorescence) DV culture medium - - 1423 Larras et al. (2013) 1423 GEOMETRIC MEAN Bacillariophyta Bacillariophyceae Microalgae (Mayamaea fossalis) Exponential Growth Phase 4 d Chronic EC05 (Cell density) DV culture medium 21 ± 2 - 74 Larras et al. (2012) 74 GEOMETRIC MEAN 74 VALUE USED IN SSD Bacillariophyta Bacillariophyceae Microalgae (Mayamaea fossalis) - 4 d Chronic EC10 (Growth fluorescence) DV culture medium - - 91 Larras et al. (2013) Bacillariophyta Bacillariophyceae Microalgae (Mayamaea fossalis) Exponential Growth Phase 4 d Chronic EC10 (Growth fluorescence) DV culture medium - - 96 Larras et al. (2013) 93.47 GEOMETRIC MEAN Bacillariophyta Bacillariophyceae Microalgae (Mayamaea fossalis) Exponential Growth Phase 4 d Chronic EC50 (Cell density) DV culture medium 21 ± 2 - 463 Larras et al. (2012) 463 GEOMETRIC MEAN Bacillariophyta Bacillariophyceae Microalgae (Mayamaea fossalis) - 4 d Chronic EC50 (Growth fluorescence) DV culture medium - - 139 Larras et al. (2013) 139 GEOMETRIC MEAN Bacillariophyta Bacillariophyceae Microalgae (Nitzschia palea) Exponential Growth Phase 4 d Chronic EC05 (Cell density) DV culture medium 21 ± 2 - 106 Larras et al. (2012) 106 GEOMETRIC MEAN 106 VALUE USED IN SSD
Bacillariophyta Bacillariophyceae Microalgae (Nitzschia palea) - 4 d Chronic EC10 (Growth fluorescence) DV culture medium - - 380 Larras et al. (2013) Bacillariophyta Bacillariophyceae Microalgae (Nitzschia palea) Exponential Growth Phase 4 d Chronic EC10 (Growth fluorescence) DV culture medium - - 196 Larras et al. (2013) 272.91 GEOMETRIC MEAN Bacillariophyta Bacillariophyceae Microalgae (Nitzschia Exponential Growth Phase 4 d Chronic EC50 (Cell density) DV culture medium 21 ± 2 - 1539 Larras et al. (2012) 1539 GEOMETRIC MEAN Bacillariophyta Bacillariophyceae Microalgae (Nitzschia palea) - 4 d Chronic EC50 (Growth fluorescence) DV culture medium - - 1667 Larras et al. (2013) 1667 GEOMETRIC MEAN Bacillariophyta Bacillariophyceae Microalgae (Sellaphora minina) - 4 d Chronic EC10 (Growth fluorescence) DV culture medium - - 693 Larras et al. (2013) Bacillariophyta Bacillariophyceae Microalgae (Sellaphora minina) Exponential growth phase 4 d Chronic EC10 (Growth fluorescence) DV culture medium - - 3218 Larras et al. (2013) 1493 GEOMETRIC MEAN 1493 VALUE USED IN SSD Bacillariophyta Bacillariophyceae Microalgae (Sellaphora minina) - 4 d Chronic EC50 (Growth fluorescence) DV culture medium - - 2606 Larras et al. (2013) Bacillariophyta Bacillariophyceae Microalgae (Sellaphora minina) Exponential growth 4 d Chronic EC50 (Growth fluorescence) DV culture medium - - 4236 Larras et al. (2013) 3322.5 GEOMETRIC MEAN Bacillariophyta Bacillariophyceae Microalgae (Stauroneis amphoroides) - 3 d Chronic EC50 (Biomass Growth Rate, AUC) ASTM Type 1 water 24 ± 2 7.5 ± 0.1 31 US EPA (2015a) 31 GEOMETRIC MEAN
Chordata Amphibia Pacific Tree Frog (Pseudacris regilla) Embryo 10 d Chronic NOEC (Mortality) Natural chlorine-free freshwater 20 7.4 29 100 Schuytema and Nebeker (1998) 29 100 GEOMETRIC MEAN Chordata Amphibia Pacific Tree Frog (Pseudacris regilla) Embryo 14 d Chronic NOEC (Length) Natural chlorine-free freshwater 20 7.4 29 100 Schuytema and Nebeker (1998) Chordata Amphibia Pacific Tree Frog (Pseudacris regilla) Larvae 14 d Chronic NOEC (Length) Natural chlorine-free freshwater 20 7.4 14 500 Schuytema and Nebeker (1998) 20 541 GEOMETRIC MEAN Chordata Amphibia Pacific Tree Frog (Pseudacris regilla) Larvae 14 d Chronic NOEC (Wet weight) Natural chlorine-free freshwater 20 7.4 29 100 Schuytema and Nebeker (1998) 29 100 GEOMETRIC MEAN Chordata Amphibia Pacific Tree Frog (Pseudacris regilla) Larvae 14 d Chronic NOEC (Dry weight) Natural chlorine-free freshwater 20 7.4 21 000 Schuytema and Nebeker (1998) 21 000 GEOMETRIC MEAN Chordata Amphibia Pacific Tree Frog (Pseudacris regilla) Larvae 14 d Chronic LC50 (Mortality) Natural chlorine-free freshwater 20 7.4 10 800 Schuytema and Nebeker (1998) Chordata Amphibia Pacific Tree Frog (Pseudacris regilla) Larvae 14 d Chronic LC50 (Mortality) Natural chlorine-free freshwater 20 7.4 19 600 Schuytema and Nebeker (1998) 14 549 GEOMETRIC MEAN Chordata Amphibia Northern Frog (Rana aurora) Larvae 14 d Chronic NOEC (Wet weight) Natural chlorine-free freshwater 20 7.4 7600 Schuytema and Nebeker (1998) 7600 GEOMETRIC MEAN Chordata Amphibia Northern Frog (Rana aurora) Larvae 14 d Chronic LC50 (Mortality) Natural chlorine-free freshwater 20 7.4 22 200 Schuytema and Nebeker (1998) 22 200 GEOMETRIC MEAN
Chordata Amphibia American Bullfrog (Rana catesbeiana) Larvae 10 d Chronic NOEC (Dry weight) Natural chlorine-free freshwater 24 7.4 7600 Schuytema and Nebeker (1998) 7600 GEOMETRIC MEAN Chordata Amphibia American Bullfrog (Rana catesbeiana) Larvae 10 d Chronic NOEC (Wet weight) Natural chlorine-free freshwater 24 7.4 14 500 Schuytema and Nebeker (1998) 14 500 GEOMETRIC MEAN Chordata Amphibia American Bullfrog (Rana catesbeiana) Larvae 10 d Chronic NOEC (Length) Natural chlorine-free freshwater 24 7.4 14 500 Schuytema and Nebeker (1998) 14 500 GEOMETRIC MEAN Chordata Amphibia American Bullfrog (Rana catesbeiana) Larvae 10 d Chronic LC50 (Mortality) Natural chlorine-free freshwater 24 7.4 29 100 Schuytema and Nebeker (1998) 29 100 GEOMETRIC MEAN Chordata Amphibia American Bullfrog (Rana catesbeiana) Larvae 14 d Chronic NOEC (Dry weight) Natural chlorine-free freshwater 24 7.4 14 500 Schuytema and Nebeker (1998) 14 500 GEOMETRIC MEAN Chordata Amphibia American Bullfrog (Rana catesbeiana) Larvae 14 d Chronic NOEC (Length) Natural chlorine-free freshwater 24 7.4 14 500 Schuytema and Nebeker (1998) 14 500 GEOMETRIC MEAN Chordata Amphibia American Bullfrog (Rana catesbeiana) Larvae 14 d Chronic NOEC (Wet weight) Natural chlorine-free freshwater 24 7.4 21 100 Schuytema and Nebeker (1998) 21 100 GEOMETRIC MEAN Chordata Amphibia American Bullfrog (Rana catesbeiana) Larvae 14 d Chronic LC50 (Mortality) Natural chlorine-free freshwater 24 7.4 29 100 Schuytema and Nebeker (1998) 29 100 GEOMETRIC MEAN Chordata Amphibia American Bullfrog (Rana catesbeiana) Larvae 21 d Chronic NOEC (Dry weight) Natural 24 7.4 7600 Schuytema and Nebeker (1998)
chlorine-free freshwater 7600 GEOMETRIC MEAN Chordata Amphibia American Bullfrog (Rana catesbeiana) Larvae 21 d Chronic NOEC (Wet weight) Natural chlorine-free freshwater 24 7.4 29 100 Schuytema and Nebeker (1998) 29 100 GEOMETRIC MEAN Chordata Amphibia American Bullfrog (Rana catesbeiana) Larvae 21 d Chronic NOEC (Length) Natural chlorine-free freshwater 24 7.4 29 100 Schuytema and Nebeker (1998) 29 100 GEOMETRIC MEAN Chordata Amphibia American Bullfrog (Rana catesbeiana) Larvae 21 d Chronic LC50 (Mortality) Natural chlorine-free freshwater 24 7.4 12 700 Schuytema and Nebeker (1998) 12 700 GEOMETRIC MEAN Chordata Amphibia African Clawed Frog (Xenopus laevis) Embryo 4 d Acute NOEC (Dry weight) Natural chlorine-free freshwater 24 7.4 21 100 Schuytema and Nebeker (1998) Chordata Amphibia African Clawed Frog (Xenopus laevis) Embryo 4 d Acute NOEC (Dry weight) Natural chlorine-free freshwater 24 7.4 14 500 Schuytema and Nebeker (1998) 17 491 GEOMETRIC MEAN Chordata Amphibia African Clawed Frog (Xenopus laevis) Embryo 4 d NOEC (Length) Natural chlorine-free freshwater 24 7.4 7600 Schuytema and Nebeker (1998) Chordata Amphibia African Clawed Frog (Xenopus laevis) Embryo 4 d NOEC (Length) Natural chlorine-free freshwater 24 7.4 14 500 Schuytema and Nebeker (1998) 10 498 GEOMETRIC MEAN Chordata Amphibia African Clawed Frog (Xenopus laevis) Larvae 4 d Chronic LC50 (Mortality) Natural chlorine-free freshwater 24 7.4 29 100 Schuytema and Nebeker (1998) 29 100 GEOMETRIC MEAN Chordata Amphibia African Clawed Frog (Xenopus laevis) Embryo 14 d Chronic NOEC (Dry weight) Natural 24 7.4 29 100 Schuytema and Nebeker (1998)
chlorine-free freshwater 29 100 GEOMETRIC MEAN Chordata Amphibia African Clawed Frog (Xenopus laevis) Embryo 14 d Chronic NOEC (Length) Natural chlorine-free freshwater 24 7.4 29 100 Schuytema and Nebeker (1998) 29 100 GEOMETRIC MEAN Chordata Amphibia African Clawed Frog (Xenopus laevis) Larvae 14 d Chronic LC50 (Mortality) Natural chlorine-free freshwater 24 7.4 8100 Schuytema and Nebeker (1998) Chordata Amphibia African Clawed Frog (Xenopus laevis) Larvae 14 d Chronic LC50 (Mortality) Natural chlorine-free freshwater 24 7.4 14 500 Schuytema and Nebeker (1998) 10 837 GEOMETRIC MEAN Cyanobacteria Cyanophyceae Cyanobacteria (Anabaena variabilis) - 12 d Chronic EC50 (Growth fluorescence) BG11 medium 25 ± 1 - 80 et al. (2011) 80 GEOMETRIC MEAN 80@ USED IN SSD Cyanobacteria Cyanophyceae Cyanobacteria (Chroococcus minor) <10 days 7 d Chronic EC50 (Cell density) MN medium without inoculants, 0.45 um filtered 25 ± 1 8.1–8.4 4.7 Bao et al. (2011) 4.7 GEOMETRIC MEAN 4.7 VALUE USED IN SSD Mollusca Gastropoda Freshwater (Physa gyrina) 15 day old young 10 d Acute NOAEL (Mortality) 0.45 um filtered well water 23 6.8 ± 0.1 29 100 Nebeker and Schuytema (1998) 29 100 GEOMETRIC MEAN Mollusca Gastropoda Freshwater Snail (Physa gyrina) 15 day old 10 d Acute (Blotted wet weight) 0.45 um filtered well water 23 6.8 ± 0.1 13 400 Nebeker and Schuytema (1998) 13 400 GEOMETRIC MEAN Mollusca Gastropoda Freshwater (Physa gyrina) 15 day old young 10 d Acute LOAEL (Blotted wet weight) 0.45 um filtered well water 23 6.8 ± 0.1 22 800 Nebeker and Schuytema (1998)
22 800 GEOMETRIC MEAN Tracheophyta Liliopsida Macrophyte (Lemna aequinoctialis) - 4 d Acute EC10 (Frond count) 0.45 mm filtered distilled water, autoclaved and Hoagland No. 2 Basal Salt Mixture 30 ± 1 6 ± 0.2 2.79 Seery et al. (in prep.) 2.79 GEOMETRIC MEAN Tracheophyta Liliopsida Macrophyte (Lemna aequinoctialis) - 4 d Acute EC50 (Frond count) 0.45 mm filtered distilled water, autoclaved and Hoagland No. 2 Basal Salt Mixture 30 ± 1 6 ± 0.2 5.55 Seery et al. (in prep.) 5.55 GEOMETRIC MEAN Tracheophyta Liliopsida Macrophyte (Lemna gibba) - 7 d Chronic NOEL (Total frond number/Growth rate/Mortality) M-Hoaglands or 20X-AAP nutrient media. ASTM type 1 water 25 ± 2 4.8–5.2 medium) and 7.5 ± 0.1 media). 2.49 US EPA 2.49 GEOMETRIC MEAN 2.49 USED IN SSD Tracheophyta Liliopsida Macrophyte gibba) - 7 d Chronic EC50 (Total frond number/Growth rate/Mortality) M-Hoaglands or 20X-AAP nutrient media. ASTM type 1 water 25 ± 2 4.8–5.2 medium) and 7.5 ± 0.1 media). 13 US EPA (2015a) 13 GEOMETRIC MEAN Tracheophyta Liliopsida Macrophyte (Lemna minor) - 7 d Chronic LOEC (Total chlorophyll) Mineral medium 25 ± 1 - 5 Teisseire et al. (1999) 5 GEOMETRIC MEAN
3.16& VALUE USED IN SSD Tracheophyta Liliopsida Macrophyte (Lemna minor) - 7 d Chronic EC50 (Total chlorophyll) Mineral medium 25 ± 1 - 25 Teisseire et al. (1999) 25 GEOMETRIC MEAN Tracheophyta Liliopsida Macrophyte minor) - 7 d Chronic EC50 (Frond count) - 24 ± 1 6.5 ± 0.2 28.3 Gatidou et al. (2015) 28.3 GEOMETRIC MEAN Tracheophyta Liliopsida Macrophyte (Lemna paucicostata) - 8 d Chronic EC50 (Frond cover area) Inorganic medium containing sucrose 25 - 10.96 Grossmann et al. (1992) 10.96 GEOMETRIC MEAN 10.96@ VALUE USED IN SSD * Data were obtained from the U.S. EPA (2015) Office of Pesticide Programs Database, with methods originating from various published studies which were unattainable, therefore detail of media, temperature and pH for those entries were unavailable. It is important to note that the U.S. EPA (2015) follows strict quality assurance and quality check procedures within their organisation to ensure only high quality ecotoxicology data are reported. It was therefore assumed the data were the equivalent of either high or acceptable quality and were therefore usable in the derivation of guideline values for diuron. 1 This species has also been called Chlorella vulgaris and is currently called Chlorella pyrenoidosa. 2 This species has also been called Raphidocelis subcapitata and is currently called Pseudokirchneriella subcapitata. @ Values were chronic EC/LC50 values that were converted to chronic NOEC/EC10 values by dividing by 5 (Warne, 2001). & Value was the geometric mean of chronic LOEC and EC50 values that were converted to chronic NOEC/EC10 values by 2.5 and 5, respectively (Warne, 2001).