Procedures with a high potential for creating aerosols are conducted. These may include centrifuging, grinding, blending, vigorous shaking or mixing, sonic disruption, opening containers of materials whose internal pressures may be different from ambient pressures High concentrations or large volumes of organisms containing recombinant DNA molecules are used. Such materials may be centrifuged in the open laboratory if sealed beads or centrifuge safety cups are used and if they are opened only in a biological safety cabinet. A properly maintained biological safety cabinet (Class I or II), will have a current, annual certification that under normal operating circumstances the unit performs to manufacturer’s specification. Face protection (goggles, mask, face shield or splatter guard) is used for anticipated splashes or sprays of infectious or other hazardous materials to the face when the microorganisms must be manipulated outside the biological safety cabinet. BL2 Laboratory Facilities All BL1 Laboratory Facility Requirements, AND Provide lockable doors for facilities that house restricted agents. Install biological safety cabinets in such a manner that fluctuations of the room supply and exhaust air do not cause the biological safety cabinets to operate outside their parameters for containment. Locate biological safety cabinets away from doors, from windows that can be opened, from heavily traveled laboratory areas, and from other potentially disruptive equipment so as to maintain the biological safety cabinets’ air flow parameters for containment. An eyewash station is readily available. Illumination is adequate for all activities, avoiding reflections and glare that could impede vision. An autoclave for decontaminating laboratory wastes is available. Biological Disposal Potentially hazardous biological/physical will be removed (from appropriately labeled containers located in biology department laboratories and appropriately packaged for disposal by JMU biology personnel specially trained in handling of these wastes. Autoclave Validation
Waste for "commercial" disposal should be put into a suitable container (preferably glass, if possible). A waste label should be filled out and attached to the container. The labeled waste container will be stored in Bioscience 1033J. See Hazards in the Chemical Laboratory or Prudent Practices for Handling Hazardous Chemicals in Laboratories for specific disposal procedures. The university environmental health coordinator will periodically contract with a chemical disposal company to dispose of unwanted and waste chemicals. Before a worker's employment ends, chemicals for which that person was responsible should be discarded. XV. Records The University Environmental Health Coordinator shall maintain an accurate record of any measurements taken to monitor employee exposures and any medical consultations/examinations including tests or written opinions required by this plan. He/she will assure that such records are kept, transferred and made available in accordance with 29 CFR 1910.20. XVI. Medical Consultation and Medical Examinations Laboratory employees who work with hazardous chemicals should be an opportunity to receive medical attention, including any follow-up examination that the examining physician determines to be necessary, under the following circumstances: Whenever an employee develops or symptoms associated with a hazardous chemical to which the employee may have been exposed in the laboratory, the employee shall be provided an opportunity to receive an appropriate medical examination. Where exposure monitoring reveals an exposure level routinely above the action level (or PEL) for an OSHA regulated substance for which there are exposure monitoring and medical surveillance requirement, medical surveillance shall be established for the affected employee as prescribed by the particular standard. Whenever an event takes place in the work area such as a spill, leak, explosion or other occurrence resulting in the likelihood of a hazardous exposure, the affected employee shall be provided an opportunity for a medical consultation. Such consultation shall be for the purpose of determining the need for a medical examination. The biology department will work in conjunction with JMU Human Resources to ensure that all medical examinations and consultations shall be performed by or under the direct supervision of a licensed physician and shall be provided without cost to the employee, without loss of pay and at a reasonable time and place. The employer should provide the following information to the physician: Identity of the hazardous chemical(s) to which the employee may have been exposed.