KURUKSHETRA UNIVERSITY, KURUKSHETRA Curriculum for M.Sc. Biotechnology (CBCS) Scheme of Examination (Effective from the Academic Session 2016-2017) Semester-1 Paper No. Nomenclature type Credits Contact hours per week Internal Marks External Marks Total Marks Duration of BT-101 Biomolecules core 4 4 20 80 100 Three BT-102 Microbiology core 4 4 20 80 100 BT-103 Molecular Cell Biology core 4 4 20 80 100 BT-104 Biotechniques core 4 4 20 80 100 Three BT-105 Lab Course based on Biomolecules and Biotechniques core 4 8 20 80 100 Six (Two sessions of hours each) BT-106 Lab Course based on Molecular cell Biology & Microbiology core 4 8 20 80 100 Six (Two sessions of three hours each) Total Credits-24 Total Marks -600 KURUKSHETRA UNIVERSITY, KURUKSHETRA Curriculum for M.Sc. Biotechnology (CBCS)
Time: 3 hrs Objectives: The purpose of the course is to teach the students about basics and advanced concepts of Molecular Genetics and ensuring that students acquire an extensive and sound knowledge for future studies. Outcomes: After the completion of the course, the students would acquire the knowledge of genome structure and organization in eukaryotes, DNA mutability, transcription regulation in prokaryotes and eukaryotes, site specific recombination and its biological role, transposition and its mechanisms. They would learn advanced techniques of genome mapping and sequencing, comparative genomics and transcriptome analysis. They would also be acquainted with methodological concepts and tools needed to acquire top-level skills in the field of molecular genetics NOTE: Nine questions will be set in all Question No. 1, which will be short answer type covering the entire syllabus, will be compulsory. The remaining eight questions will be set unit-wise with two questions from each unit. The candidates will be required to attempt Question No. 1 and four others selecting one question from each unit. All questions will carry equal marks. Unit – I Eukaryotic Genome Structure and Organization Genome sequence and chromosome diversity, variation in chromosome number, Special features of metaphase chromosomes, Chromosome banding, Genome size and complexity, organization and content of human genome, Repetitive DNA, Microsatellites, genome wide repeats, Split genes, overlapping genes, cryptic genes, Retrogenes, Multigene families, Pseudo genes Nucleosome-Basic Structure, spatial arrangements of histones, chromatosome, Solenoid model, Chromatin domains, Chromatin modifications The Mutability of DNA An overview of mutation and polymorphism, VNTR polymorphism, DNA damage- spontaneous, Induced (Alkylation, oxidation, radiation), Genotoxicity/ mutagenicity test systems - Ames test, Sister chromatid exchanges, Micronucleus, Comet assay Unit – II Transcription Regulation in Prokaryotes
1. Nine questions will be set in all 2. Question No. 1, will be short answer type covering the entire syllabus, will be compulsory. The remaining eight questions will be set unit-wise with two questions from each unit. The candidates will be required to attempt Question No. 1 and four others selecting one question from each unit. 3. All questions will equal marks. Unit-I Biosafety: Introduction; Historical background; Biosafety in the laboratory; Laboratory associated infections and other hazards;Biosafety management for environmentally safe use of biotechnology; Biosafety guidelines; Recommended Biosafety Levels for Infectious Agents and Infected Animals; Definition of GMOs & LMOs; Good manufacturing practices (GMP) and Good lab practices( GLP); Overview of National Regulations and relevant International Agreements including Cartagena Protocol; Roles of Institutional Biosafety Committee ( IBSC), RCGM, GEAC, MEC, SBCC, DLC and RDAC; Guidelines for research in transgenic sciences and release of GMOs to environment; Bioterrorism and convention on biological weapons Unit-II Bioethics: Ethical issues related to biotechnology research; Ethical issues associated with consumptions of genetically modified foods and other products, Ethical implications of human genome project, Social and ethical implications of biological weapons, Bioremediations and environmental impacts of using GMOs; Ethics of patenting- and its impact on biodiversity rich developing countries; Use of animals for research and testing and Alternatives for Animals in Research. Unit-III Social, economical and issues related to biotechnology: Public education of the processes of biotechnology involved in generating new forms of life for informed decision making; Testing of drugs on human volunteers; Human cloning and Gene therapy - ethical and social issues; Organ transplantation- ethical and legal implications; Research focus to address the need of the poor and of environment. Entrepreneurship: Potential entrepreneurship activities in biotechnology, product development, marketing, research and training units. Industrial licensing, venture capital, Biotechnological industries in India and potential job opportunities. Unit- IV