Practice using dissecting scope to differentiate contamination in struck hits Record ideas and experimental procedure in Lab notebook Sept 27 – Oct 2 1) Re-streak all hits two more times onto fresh plates; use dissecting scope to be sure purifying Oct 3 Midterm 1) Make frozen stocks of hits from 1st round of co-culturing Prepare for midterm Oct 9 Streak out possible hits from freezer Oct 10 1) Discuss possible additional reporters TA will summarize phenotypes Discuss possible additional treatments? Get new reporters and set up co-culture screen Set up PCR of existing hits Set up secondary screen of hits from 1st round TA will put PCR reactions into 4 degrees either this evening or tomorrow morning Read 16S identification and bacterial phylogeny Work on lab report from Sept 26 experiment Oct 11 Come in to record results of secondary screen Oct 16 1) Plate new co-cultures with new reporters Oct 17 1) Turn in Lab Report Kit purify PCR reactions (only those that passed secondary screen) Run DNA gel of PCR reactions (Quantify?) Send off DNA for sequencing (only those that have sufficient DNA) Screen co-culture plates with new reporters/conditions and pick and streak additional hits Read about BLAST (link on Sakai) Oct 18 – Oct 23 1) Re-streak all hits two more times onto fresh plates; use dissecting scope to be sure purifying different colony types Oct 23 Set up secondary screen of new hits from additional reporter co-cultures Streak out hits from first round Oct 24 1) Examine secondary screen from new hits 2) Do PCR of new hits Need laptops for DNA analysis
Lab Exercises: Assignments related to the readings and your research will be collected in class. In-lab assignments and quizzes will be given. You will receive 5 points for participating each week in discussions and lab work in addition to keeping a lab notebook. Mid-term: One exam for the course will focus on the assigned readings, PowerPoint slides, homework, learning outcomes, quizzes, lab reports, and in-class assignments. Test materials to study: lab notebook, lab exercises, reading, slides, and learning outcomes. Presentation: The presentation will replace a final exam. You will present your findings to the rest of the class which includes a poster presentation. Items to bring to class each week: Lab notebook (composition book), computer, writing utensils, creativity Grading: Biology 102L Assignments Topic Assignment Due Microbes, Microscopy, Identifying Bacteria Gram Stain and Sterile Technique protocols written in Lab Notebook – 2pts Bacteria Identification – 5pts Scientific Analysis, Microscopy continued, Graph Sample Data Historical Microbiologist Presentations – 4pts Scientific Paper Section Discussions – 4pts Graph in Excel – 6pts Serial Dilutions, Practice Plate Streaking, Special Talk Quiz - 3pts Treatment of Soil Lab Notebook Checked – 5pts Lab Report Outline Due – 4pts Midterm Midterm – 30pts Pick and Streak Practice, Fluorescence Microscopy, Begin Coculture Plate Practice Lab Notebook Checked – 5pts Revise Lab Reports, Replate mixed cocultures Take pictures of possible inducing microbes – 2pts Lab Work Lab Report Due – 20pts Lab Notebook Checked – 5pts Lab Work Lab Notebook Checked – 5pts Final Day Poster Presentation – 50pts The final exam will be replaced by the presentation the last week of the lab. Grade Scale: 87-89 B+ 77-79 C+ 67-69 D+ 93-100 A 83-86 B 73-76 C 60-66 D 90-92 A- 80-82 B- 70-72 C- <60 F Final grades will be assigned on the total number of points at the end of the semester.