Supplemental Table S4. Analytical validation specimens. Sample ID Type Cell Line/Source 5' Gene 5' Transcript 5' Exon 3' Gene 3' Transcript 3'Exon Status SpARKFuse1 Cell Line HEK293FT Transfection BCR NM_004327 4 NM_001174065 10 True Positive SpARKFuse2 Cell Line HEK293FT Transfection FGFR2 NM_001144913 16 KIAA1967 NM_021174 4 True Positive SpARKFuse3 Cell Line HEK293FT Transfection FGFR2 NM_001144913 16 MGEA5 NM_001142434 11 True Positive SpARKFuse4 Cell Line HEK293FT Transfection FGFR2 NM_001144913 16 TACC3 NM_006342 16 True Positive SpARKFuse5 Cell Line HEK293FT Transfection FGFR2 NM_001144913 16 AFF3 NM_001025108 7 True Positive SpARKFuse6 Cell Line HEK293FT Transfection NM_001144913 16 CASP7 NM_001267056 2 True Positive SpARKFuse7 Cell Line HEK293FT Transfection BAG4 NM_004874 2 FGFR1 NM_001174066 5 True Positive SpARKFuse8 Cell Line HEK293FT Transfection CCDC NM_005436 1 RET NM_020630 12 True Positive SpARKFuse9 Cell Line HEK293FT Transfection ERLIN2 NM_007175 8 FGFR1 NM_001174065 2 True Positive SpARKFuse10 Cell Line HEK293FT Transfection FGFR2 NM_001144913 16 NM_005436 2 True Positive SpARKFuse11 Cell Line HEK293FT Transfection FGFR2 NM_001144913 16 OFD1 NM_003611 3 True Positive SpARKFuse12 Cell Line HEK293FT Transfection KIF5B NM_004521 15 RET NM_020630 12 True Positive SpARKFuse13 Cell Line HEK293FT Transfection NM_005437 6 RET NM_020630 12 True Positive
SpARKFuse67 Fresh Frozen OSU13053 FGFR2 NM_001144913 16 CCDC6 NM_005436 2 True Positive SpARKFuse68 Frozen OSU13053 FGFR2 NM_001144913 16 KIAA1598 NM_001258300 8 True Positive SpARKFuse69 Fresh Frozen OSU13053 C9orf3 NM_032823 11 SYK NM_003177 4 True Positive SpARKFuse70 Fresh Frozen OSU13053 NM_001163213 17 RBBP4 NM_001135255 12 True Positive SpARKFuse71 Fresh Frozen OSU13053 HNRNPA2B1 NM_002137 7 ETV1 NM_001163150 2 True Positive SpARKFuse72 Fresh Frozen OSU13053 FGFR2 NM_001144913 16 INA NM_032727 2 True Positive SpARKFuse73 Frozen OSU13053 NM_004521 15 RET NM_020630 11 True Positive SpARKFuse74 Fresh Frozen OSU13053 KLK2 NM_005551 1 NM_001144913 4 True Positive SpARKFuse75 Cell Line GM18965 NA True Negative SpARKFuse76 Cell Line GM18515 NA True Negative SpARKFuse77 Cell Line GM12815 NA True Negative SpARKFuse78 Cell Line GM18960 NA True Negative SpARKFuse79 Cell Line GM12750 NA True Negative SpARKFuse80 Cell Line GM12872 NA True Negative SpARKFuse81 Cell Line GM12978 NA True Negative SpARKFuse82 Cell Line GM18488 NA True Negative SpARKFuse83 Cell Line GM18506 NA True Negative SpARKFuse84 Cell Line GM18511 NA True Negative
SpARKFuse85 Cell Line GM18572 NA True Negative SpARKFuse86 Cell Line GM18573 NA True Negative SpARKFuse87 Cell Line GM18595 NA True Negative SpARKFuse88 Cell Line GM18854 NA True Negative SpARKFuse89 Cell Line GM18957 NA True Negative SpARKFuse90 Cell Line GM18961 NA True Negative SpARKFuse91 Cell Line GM19240 NA True Negative SpARKFuse92 Cell Line GM10840 NA True Negative SpARKFuse93 Cell Line HL60 NA True Negative SpARKFuse94 Cell Line GM12878 NA True Negative SpARKFuse95 FFPE OSU-TAS NA True Negative SpARKFuse96 FFPE OSU-TAS NA True Negative SpARKFuse97 FFPE OSU-TAS NA True Negative SpARKFuse98 FFPE OSU-TAS NA True Negative SpARKFuse99 FFPE OSU-TAS NA True Negative SpARKFuse100 FFPE OSU-TAS NA True Negatives SpARKFuse101 FFPE OSU-TAS NA True Negatives SpARKFuse102 FFPE OSU-TAS NA True Negatives
SpARKFuse103 FFPE OSU-TAS NA True Negatives SpARKFuse104 FFPE OSU-TAS NA True Negatives SpARKFuse105 FFPE OSU-TAS NA True Negatives SpARKFuse106 FFPE OSU-TAS NA True Negatives SpARKFuse107 FFPE OSU-TAS NA True Negatives SpARKFuse108 FFPE OSU-TAS NA True Negatives SpARKFuse109 FFPE OSU-TAS NA True Negatives SpARKFuse110 FFPE OSU-TAS NA True Negatives Summary of 110 samples used for the analytic validation of OSU-SpARKFuse. Information regarding specimen type, source, and fusion involved genes for all samples are included. The status of each sample as determined during the validation is also indicated. OSU-TAS, Ohio State University Tissue Archive Service; OSU-13053, patient tissue derived under clinical research protocol; CMCL, Colorado Molecular Correlates Laboratory; NA, not applicable.