BIOSAFETY QUIZ Name: _________________________________ Date: __________________________________ Personal protective equipment (PPE) for each task is: Used at the discretion of the employee Designated by the supervisor and specified by the exposure control plan (ECP) and / or by any site-specific standard operating procedures (SOPs) Not necessary unless the task is labeled as “high risk” Not required for students Which of the following statements about Personal Protective Equipment (PPE) are correct? PPE must be worn and stored only inside the laboratory PPE must be chosen based upon the work being completed Employees utilizing PPE must be properly trained All of the The main purpose of a laboratory coat is to: Keep your clothes from being ruined Help assess the cleanliness of the laboratory Identify you as a trained professional Keep contamination off your clothing Give you access to several pockets at once Laboratory coats must be taken home to be bleached and cleaned on a regular basis True False It is okay to wear sandals in the lab as long as you also wear socks True False Valid method(s) of compliance is / are: Eating, drinking, smoking or applying cosmetics in the work area Pipetting by mouth Washing hands before leaving the work area Wearing the appropriate personal protective equipment (PPE) C and D above An ideal container for biomedical waste would: Be hard-sided Have a foot operated lid Be easily decontaminated All of the The following material may be disposed of in the regular trash: Animal tissue, blood, dander or droppings Human blood, tissue, or body fluids Broken glass None of the above How should biological materials that need to be transported from the lab to another location be handled? Wear a lab coat and transport materials in your pocket Wear gloves and carry the material in your hands Seal materials in a sturdy, leak-proof secondary container Cells in cell culture flasks and /or dishes are fine for transport
It is appropriate to wear two gloves in the hall/ elevator, if you are carrying samples True False Which type of disposable glove provides the greatest tear and puncture resistance? Nitrile Vinyl If gloves are worn when handling viable, potentially infectious material, should be no need for hand washing. True False Alcohol-based hand sanitizers are just as effective as hand washing for removal of a broad-spectrum of infectious organisms. True False If you notice that a sharps container is getting full, you should: Empty the sharps container into biohazard bag Obtain a new one, close the lid on the full one and arrange to have it safely transported for incineration Empty the sharps container into a properly lined, leak proof, puncture proof container Recap the needles and throw them in the regular trash Remove the needle from the syringe before you put it in the sharps container. True False Safe Sharps practices refers to: Plastic ware substituted for glassware Needle designs engineered to minimize needle stick injuries Discouraging the practice of recapping needles All of the above Which of the following materials require compliance with federal regulations for commercial carrier / air transport of dangerous goods: Dry ice Pathogenic cultures Chemical preservatives All of the above Biohazard labels or stickers are required on: Storage and transport containers/equipment used for blood or OPIM Biomedical waste containers Contaminated linen/laundry bins All of the above A Biosafety Level 2 (BSL-2) lab works with agents that may be described as: Not associated with disease in healthy adult humans Associated with human disease which is rarely serious and for which preventive or therapeutic interventions are often available Associated with serious or lethal human disease for which preventive or therapeutic interventions may not be available but are not readily transmissible to others (high individual risk but low community risk)
Biological Safety Cabinets used for infectious / potentially infectious material must be certified at least: Daily Monthly Yearly Once Which of the following protect both the user and the material that is handled inside the cabinet Fume hood Clean bench Biological safety cabinet (BSC) All of the 30. In a II, Type A2 biological safety cabinet (typical unit found in most labs): Downward laminar flow splits 2-6 inches above work surface between front and rear grills HEPA-filtered air from the cabinet is in the unit and also exhausts to the room It is safe to work with substantial amounts of chemicals and radiolabeled nucleotides A and B only 31. Open flames are not allowed in a biological safety cabinet because They disrupt airflow patterns that protect user and product They can damage HEPA filters They pose a fire hazard All of the above Acceptable alternatives to Bunsen burner use in the biological safety cabinet (BSC) include: Disposable loops, spreaders, or glass beads Bacticinerator Touch-O-Matic burner All of the above It’s okay to pipette tips and microcentrifuge tubes in the biological safety cabinet as long as they are pushed up against the back wall of the cabinet. True False Which of the following practices should be utilized when working in a biological safety cabinet? Disinfect all surfaces of the BSC before and work Disinfect all items which go into and come out of the BSC Allow the BSC to operate before work begins and after work ceases All of the above a spill should occur within a biological safety cabinet (BSC), what steps would immediately be taken? A. Turn off the BSC, grab the biological spill kit, and immediately wipe up the spill with a dry, absorbent towel Turn off the BSC and pour a germicidal agent on the spill Turn off the BSC, evacuate the laboratory, and notify company safety authorities Leave the BSC on, grab the biological spill kit, add absorbent material and germicidal agent, wipe up the spill once contact time has permitted inactivation, and remove corrosive residue with 70% ethanol. Leave the BSC on, grab the biological spill kit and wipe up the spill with a dry, absorbent towel 36. Disinfectants decontaminate soiled and clean surfaces equally well. True False