Relevant Contact Information BROCK PHONE BOARD: 905 688 5550 EXTENSION EMAIL Campus Security Services- Emergency 3200 security@brocku.ca Campus Security Services- Non-Emergency 4300 security@brocku.ca Chair, Biosafety Sub-committee- Alan Castle 3598 acastle@brocku.ca Biosafety Officer- Vistorte 6179 lvistorte@brocku.ca Health, Safety & Wellness 7233 besafe@brocku.ca CL2 Faculty Representative- Litsa Tsiani 3881 etsiani@brocku.ca CL3 Faculty Representative- Fiona Hunter 3394 fhunter@brocku.ca Animal Care 5820 dcarlson@brocku.ca Jacinta Dano- Biosafety Coordinator-Biology 4968 Greig Inglis- Biosafety Coordinator- Kinesiology 4667 ginglis@brocku.ca Jose Gabrie- Biosafety Coordinator- Health Sciences 6635 jgabrie@brocku.ca John Ciolfi- Senior Lab Demonstrator- Biology 3763 jciolfi@brocku.ca Facilities Management 3717 facilitiesmanagement@brocku.ca Machine Shop 3420 scrumb@brocku.ca Electronic Shop 3419 tmacdonald@brocku.ca Membership of the Biosafety Sub-committee Core Members: Chair; 1 CL3 permit holder (faculty member) 1 CL2 permit holder (faculty member) 1 CL2 member (senior demonstrator or research technician) Biosafety Officer (BSO) 1 community representative with experience relevant to biosafety Administrative Assistant
Hydrogen peroxide 3% — low disinfectant. Cleans floors, walls and furnishings. 6.5% — high level disinfectant. Effective for high level disinfection of flexible endoscopes. Higher (35%) concentrations used as sterilant in specially designed machines for decontamination of devices, and for room decontamination in high containment facilities. Strong oxidant. Fast acting. Breaks down into water and oxygen. Can be corrosive to aluminum, copper, or zinc. Iodophors Intermediate level disinfectant for some equipment. Low level disinfectant for hard surfaces and equipment that does not touch mucous membranes. Rapid action. Relatively free of toxicity and irritancy, but may cause allergy. Note: Antiseptic iodophors are NOT suitable for use as hard surface disinfectants. Corrosive to unless combined with inhibitors. Disinfectant may burn tissue. Inactivated by organic materials. May stain fabrics and synthetic materials. Per acetic acid High level disinfectant or sterilant for heat sensitive equipment. Higher concentrations used as sterilants in specially designed machines for decontamination of heat sensitive medical devices. Innocuous decomposition (water, oxygen, acetic acid, hydrogen peroxide). Rapid action at low temperature. Active in presence of organic materials. Corrosive to tissue. Unstable when diluted. Concentrated solution is flammable and can be violently reactive. Phenolic Low/intermediate disinfectants. Clean floors, walls, benches, and furnishings. Clean hard surfaces and equipment that does not touch mucous membranes. Leaves residual film on environmental surfaces. Commercially available with detergents to provide one-step cleaning and disinfecting. Not recommended for use on food contact surfaces. May be absorbed through skin or by rubber. Some synthetic flooring may become sticky with repetitive use. Quaternary ammonium compounds Low disinfectant. Clean floors, walls, benches and furnishings. Generally to hands. Usually have detergent properties. DO NOT use to disinfect medical instruments. Non-corrosive. Limited use as disinfectant because of narrow microbicidal spectrum.
Table - Advantages and disadvantages of currently available sterilization methods Method Parameters Monitoring Frequency Use/Advantages Disadvantages Steam a) Small table top sterilizers b) Gravity displacement sterilizers including sterilizers c) vacuum sterilizers Raised pressure (preset by manufacturer) to increase temperature to 1210 C (1330 C for flash sterilizers) Time varies with temperature, type of material and whether the instrument is wrapped or not. Steam must be saturated (narrow lumen items may require pre- humidification). Air detection for vacuum sterilizers- before first cycle of day Mechanical - each cycle; Chemical - each cycle; Biologic - at weekly, but preferably daily, and with each load of critical items (Bacillus stearothermophilus spores). Heat tolerant instruments, labware, and accessories. Linen. Inexpensive. Rapid. Efficient. Can be used to sterilize liquids. Unsuitable for anhydrous oils, powders, lensed instruments, heat and moisture sensitive materials. Some table top sterilizers lack a drying phase. Flash sterilization Flash sterilization should be used only in an emergency. Flash sterilization should never be used for critical items such as implantable devices. Mechanical - each cycle; Chemical - each cycle; Biologic - at least once a week but preferably daily. Not recommended Difficult to monitor. The efficacy of flash sterilization will be impaired if all the necessary parameters are not met properly (e.g., time, temperature), the device is contaminated with organic matter, air is trapped in or around the device, or the sterilizer or flash pack is not working properly. Sterility cannot be maintained if the device is not wrapped. Boiling Not recommended None Not recommended Unable to be monitored; unreliable Microwave ovens Not recommended None Not recommended Unable to be monitored. Unreliable Glass bead sterilizers Not recommended None Not recommended Unable to be monitored. Cold spots. Inconsistent heating. Trapped air.
Figure - Biohazard Signage Template (sticker available at Science Stores; fillable pdf available at SPECIMEN INVENTORY BUILDING: │ LAB # │ PI: Date Received Initial Pathogenic Material (Tissue, Culture) Quantity Volume (ml) Mass (g) Date Removed Initial