. Supplementary Table 1: Peptide Sequence1 m/z Mass Error (ppm)2 RT Protein PTM Bioactivity Homologous Bioactive Peptide3 Bioactive peptide database VEELKPTPEGDLE 728.3671 3.9 15.58 β-Lg Hypocholesterolemic VYVEELKPTPEGDLEILLQK MBPDB TIASGEPTSTPTTE 696.3335 4.4 13.84 Κ-cas stimulates exocrine pancreatic secretion, immunomodulatory, Antimicrobial and ACE-inhibitory MBPDB TVMENFVAFVDK 700.3527 4.0 22.05 SA - - MBPDB/BIOPEP VYPFPGPIPN 550.7943 3.5 21.09 β-cas Antioxidant and VYPFPGPIPN MBPDB VYPFPGPIPN ACE-inhibitory VYPFPGPIPNSLPQNIPP MBPDB VYPFPGPIPN ACE-inhibitory LVYPFPGPIPNSLPQNIPP MBPDB VYPFPGPIPN ACE-inhibitory LVYPFPGPIPNSLPQN MBPDB VYPFPGPIPN ACE-inhibitory LVYPFPGPIPNSLPQ MBPDB VYPFPGPIPN ACE-inhibitory DELQDKIHPFAQTQSLVYPFPGPIPNS MBPDB 652.6587 1.0 21.53 SA - - DIQKVAGTWYSL 690.8622 1.0 20.79 β-Lg - - MBPDB/BIOPEP 527.2919 3.8 14.81 - - MBPDB/BIOPEP VIESPPEIN 499.2657 4.1 16.13 Κ-cas Stimulates exocrine pancreatic secretion, immunomodulatory, MBPDB inhibitory 1
antimicrobial and LVRTPEVDDEALE 743.3775 3.1 16.45 β-Lg - - MBPDB/BIOPEP LVRTPEVDDE 586.7957 4.1 13.82 β-Lg - - MBPDB/BIOPEP SGEPTSTPTTE 553.7476 2.8 11.68 Κ-cas Stimulates exocrine pancreatic secretion, immunomodulatory, antimicrobial and MBPDB AFDEKLFTF 559.2846 5.0 21.97 SA - - 941.0401 5.2 24.43 β-cas Immunomodulatory, antithrombin, antimicrobial and YQEPVLGPVRGPFPIIV MBPDB ACE-inhibitory LLYQEPVLGPVRGPFPIIV MBPDB 841.4514 3.7 20.42 β-Lg Hypocholesterolemic VYVEELKPTPEGDLEILLQK MBPDB ELKPTPEGDLE 614.3116 4.7 14.61 β-Lg Hypocholesterolemic VYVEELKPTPEGDLEILLQK MBPDB 538.6191 2.9 15.15 β-Lg - - MBPDB/BIOPEP VRTPEVDDEALE 686.8345 2 15.45 β-Lg - - MBPDB/BIOPEP 623.8234 3 24.53 SA CAM - - MBPDB/BIOPEP VRTPEVDDE 530.2527 2.6 12.49 β-Lg - - DAFLGSFLYEYSR 784.3763 1.8 23.55 SA - - IIAEKTKIPAVF 443.9443 3.2 17.98 β-Lg Antimicrobial IIAEKTKIPAVF MBPDB 518.89 1.7 15.5 SA CAM - - MBPDB/BIOPEP LKPTPEGDLE 549.7899 4.3 13.93 β-Lg Hypocholesterolemic VYVEELKPTPEGDLEILLQK MBPDB DPP-IV Inhibitor LKPTPEGDLEIL MBPDB
KFDKALKALPM 421.2507 3.2 16.33 β-Lg - - MBPDB/BIOPEP VIESPPEIN(+.98) 998.4407 -63.5 16.72 Κ-cas DeAm (NQ) BIOPEP STPTTE 635.3052 26.6 4.33 Κ-cas stimulates exocrine pancreatic secretion, immunomodulatory, antimicrobial and MBPDB 784.9221 -1.5 23.98 β-cas Ox (M) ACE-inhibitory LPQNIPPLTQTPVVVPPFLQPEVMGVSK KIPAVF 337.7172 5.2 17.34 β-Lg Antimicrobial IIAEKTKIPAVF MBPDB 658.0518 12.9 25.87 β-cas ACE-inhibitory LPQNIPPLTQTPVVVPPFLQPEVMGVSK MBPDB AMAASDISLL 496.3247 130.5 21.21 β-Lg Antimicrobial SLAMAASDISLL MBPDB DAQSAPL 701.3478 2.1 13.62 β-Lg Antimicrobial DAQSAPLR Antimicrobial AASDISLLDAQSAPLR MBPDB ACE-inhibitory LDAQSAPLR MBPDB ACE-inhibitory DAQSAPLRVY EMPFPKYPVEPF 740.8639 1.6 21.92 β-cas protective effects in indomethacin-induced enteritis through preservation of goblet cells and improvement in wound healing, opioid, increases mMuc4 and MUC2 expression and small intestinal goblet cell density MBPDB NSAEPE 646.2825 22.8 3.47 β-Lg - -
DPP-IV Inhibitor LKPTPEGDLE MBPDB DPP-IV Inhibitor LKPTPEGDL MBPDB Antimicrobial VEELKPTPEGNLE MBPDB 510.2563 3.2 16.22 Κ-cas Na ad. Stimulates exocrine pancreatic secretion, immunomodulatory, antimicrobial and MBPDB 559.3714 47.3 12.87 β-Lg - - MBPDB/BIOPEP LSFNPT 678.35 6.3 16.69 β-Lg ACE-inhibitory LSFNPTQ MBPDB SQSKVLPVPQ 541.8646 93.9 15.03 β-cas ACE-inhibitory SQSKVLPVPQ MBPDB ACE-inhibitory PPQSVLSLSQSKVLPVPQ MAASDISLL 920.4788 3.4 20.97 β-Lg Antimicrobial SLAMAASDISLL MBPDB DDSPDLPK 443.7133 4.7 12.95 SA - - MBPDB/BIOPEP 571.293 -11.8 17.14 Κ-cas Biot Stimulates exocrine pancreatic secretion, immunomodulatory, antimicrobial and MBPDB Antimicrobial MAIPPKKNQDKTEIPTINT MBPDB AVEST 506.2996 106.7 2.82 Κ-cas Stimulates exocrine pancreatic secretion, immunomodulatory, antimicrobial and MBPDB
PEVIESPPEIN 612.3427 51.3 20.61 Κ-cas Stimulates exocrine pancreatic secretion, immunomodulatory, antimicrobial and NNDSTE 679.2606 11.3 1.62 α-la DPP-IV Inhibitor IVQNNDSTEYGLF MBPDB DTDYKKYLLF 653.3583 28.2 20.7 β-Lg stimulates proliferation VLVLDTDYKKYLLF MBPDB AFEVV 564.3454 75.7 17.63 Antimicrobial NLENTVKETIKYLKSLFSHAFEVVKT 540.7841 3.5 15.11 β-Lg Dehy Hypocholesterolemic VYVEELKPTPEGDLEILLQK MBPDB DPP-IV Inhibitor LKPTPEGDLEIL MBPDB DPP-IV Inhibitor LKPTPEGDLE 575.6592 74.5 23.55 SA CAM (DHKE - - MBPDB/BIOPEP 1
SPPEIN 656.3348 15.0 12.46 Κ-cas Stimulates exocrine pancreatic secretion, immunomodulatory, antimicrobial and MBPDB KIIAE 573.3972 63.9 10.79 β-Lg - - MBPDB/BIOPEP CVLLLA 631.3931 13.2 20.15 - - MBPDB/BIOPEP 718.3918 5.9 22.41 β-Lg Dehy - - MBPDB/BIOPEP 473.2954 7.1 18.18 β-Lg Acet Antimicrobial IIAEKTKIPAVF ENSAEPE 775.2869 -30.4 5.13 β-Lg - - MBPDB/BIOPEP 1
GTWYSL 726.336 -13.4 20.22 β-Lg - - MBPDB/BIOPEP 627.6472 3.2 17.6 SA CAM - - AIVQN 544.354 83.0 10.02 α-la - - MBPDB/BIOPEP K(sub E)VIESPPEIN 563.3137 4.6 14.86 Κ-cas Mut - - MBPDB/BIOPEP 501.7842 -4.1 13.29 β-Lg Ox (M) - - MBPDB/BIOPEP TLPDTEKQIKKQ 476.9414 2.8 11.39 SA - - MBPDB/BIOPEP NAVPITPT 812.4165 -42.8 15.25 α-s2-cas - - MBPDB/BIOPEP KDLKGY 362.2067 3.5 7.51 α-la - - MBPDB/BIOPEP LVYPFPGPIH(sub P)N 627.3391 2.6 19.98 β-cas Mut - - MBPDB/BIOPEP 632.3351 33.8 16.12 β-cas RELEELNVPGEIVESLSSSEESITR 719.3517 -10.2 16.77 β-Lg Dehy Hypocholesterolemic VYVEELKPTPEGDLEILLQK
SFNPT 565.3006 69.0 12.8 β-Lg ACE-inhibitory LSFNPTQ MBPDB 636.3602 3.3 13.69 Κ-cas Stimulates exocrine pancreatic secretion, immunomodulatory, antimicrobial and 554.7827 5.5 20.03 Κ-cas E→pE Stimulates exocrine pancreatic secretion, immunomodulatory, antimicrobial and MBPDB 534.7853 5.8 17.15 Κ-cas Prop (K Stimulates exocrine pancreatic secretion, immunomodulatory, antimicrobial and MBPDB
ALNEN 560.3153 85.5 4.05 β-Lg Stimulates proliferation and antimicrobial IDALNENK GDLEIL 659.3644 5.2 19.94 β-Lg Hypocholesterolemic VYVEELKPTPEGDLEILLQK MBPDB DPP-IV Inhibitor LKPTPEGDLEIL 597.3045 2.3 19.88 SA - - MBPDB/BIOPEP EIPTIN 686.3723 0.6 15.82 Κ-cas Stimulates exocrine pancreatic secretion, immunomodulatory, antimicrobial and MBPDB SITRIN 352.2097 3.5 11.28 β-cas - - MBPDB/BIOPEP 742.3617 0.0 17.89 β-Lg For Hypocholesterolemic VYVEELKPTPEGDLEILLQK MBPDB 507.8652 123.2 18.03 β-Lg E→pE Antimicrobial IIAEKTKIPAVF
DTDYKKYL 349.1799 5.0 15.54 β-Lg stimulates proliferation VLVLDTDYKKYLLF MBPDB LNENKVL 415.2368 -13.8 13.44 β-Lg - - MBPDB/BIOPEP 1002.385 -77.1 16.55 β-Lg Me DPP-IV Inhibitor TPEVDDEALEK MBPDB NLPPL 553.3823 86.6 17.78 SA - - NIPPL 553.3823 86.6 17.78 β-cas DPP-IV Inhibitor PQNIPPL MBPDB DPP-IV Inhibitor LPQNIPPLT MBPDB ACE-inhibitory NIPPLTQTPVVVPPFLQ ACE-inhibitory NIPPLTQTPV MBPDB ACE-inhibitory LPQNIPPLTQTPVVVPPFLQPEVMGVSK MBPDB 500.9878 129.9 14.35 β-Lg - - VLPVPQ 652.422 29.5 16.07 β-cas Antioxidant, antimicrobial and VLPVPQK MBPDB Antioxidant KVLPVPQK MBPDB
Antimicrobial VLPVPQKAVPYPQR ACE-inhibitory SQSKVLPVPQ MBPDB ACE-inhibitory SKVLPVPQ MBPDB ACE-inhibitory PPQSVLSLSQSKVLPVPQ MBPDB ACE-inhibitory KVLPVPQ MBPDB 575.3663 63.3 8.89 β-cas Immunomodulatory, antithrombin, antimicrobial and YQEPVLGPVRGPFPIIV MBPDB Antimicrobial YQEPVLGPVRGPFPI MBPDB ACE-inhibitory VRGPFPIIV MBPDB ACE-inhibitory VRGPFP MBPDB ACE-inhibitory VLGPVRGPFP MBPDB ACE-inhibitory QEPVLGPVRGPFPIIV MBPDB ACE-inhibitory LLYQEPVLGPVRGPFPIIV MBPDB ACE-inhibitory EPVLGPVRGPFP
SLPQNIPPL 489.8525 139.0 20.32 β-cas - - MBPDB/BIOPEP KALPM 559.3657 69.0 12.81 β-Lg stimulates proliferation ALKALPMHIR MBPDB DPP-IV Inhibitor, LKALPMH MBPDB ACE-inhibitory KALPMHIRLAF MBPDB EDSPEVIESPPEIN 777.8578 -5.5 18.25 Κ-cas Stimulates exocrine pancreatic secretion, immunomodulatory, antimicrobial and TSTPT 506.3092 125.8 2.99 Κ-cas Stimulates exocrine pancreatic secretion, immunomodulatory, antimicrobial and MBPDB SDISLL 647.3751 21.7 19.57 β-Lg Antimicrobial SLAMAASDISLL MBPDB