NB: If you use image analysis for other work than variety testing (seed testing, checks for purity in maintenance, etc.) and you are willing to give information, please do so. 11, 2012 VAN DER HEIJDEN, with copies to: Biometris, Wageningen-UR Droevendaalsesteeg 1 6708PB, NL-Wageningen NETHERLANDS Tel: +31 317 480 750 Fax: +31 317 483 554 E-mail: gerie.vanderheijden@wur.nl MARKKANEN, Sami Control Department Seed Certification Unit Finnish Food Safety Authority EVIRA FIN-32201 Loimaa, Finland Tel : +358 7829 45 43 Fax : +358 77 25 317 E-mail: sami.markkanen@evira.fi UPOV 34, chemin des Colombettes P.O.Box 16 CH-1211 Geneva 20 SWITZERLAND Tel: (41) 22 338 9111 Fax : (41) 22 733 03 36 E-mail : upov.mail@upov.int [Appendix II follows]
Sceye 3rd generation, proprietary light system common PC common PC 2. Software recording 2. Software processing 2. Software display/print 3. Commercially available 4. Available to members Sceye Matlab computational system, core + Image analysis toolbox outputs of Matlab are saved as common image files, no special software is required Capture: Process: partially, added own No 5. Recording conditions 6. Volume of data 7. Other remarks obtained in dark chamber, resolution 300 pt, calibration using coin. Per year: Pea 5 GB, Oilseed rape 20 GB. Image analysis in the is still under development, primary goal: resting suitable characteristics for pea and oilseed rape Application: Rape seed: Cotyledon, Petals and Siliqua Barley: Ear length, Ear length of awns Wheat. Ear length Cost/saving: Total cost for hard and software of applying image analysis are approximately 20.000 euros. Cost savings are mainly obtained in the image analysis of Rape seed characteristics of the siliqua. An advantage using image analysis is that it is possible to retrieve the image of the actual recorded data. Technical: 1. Hardware camera 1. Hardware processing 1. Hardware display PC and flatbed scanner (plustec A3) PC PC 2. Software recording 2. Software processing 2. Software display/print 3. Commercially available 4. Available to other members
Videometer image analysis progra Videometer image analysis progra Microsoft picture viewer No, specifically developed Possible to buy if you contact: http://videometer.com/ with a reference to The Danish AgriFish Agency, Department of Variety Testing 5. Recording conditions 6. Volume of data 7. Other remarks The material samples (cotyledon and petals) shall be fixed on paper with adhesive plastic foil. The paper has preprinted barcode, plot number etc.. The paper with the fixed the material are put in the flatbed scanner. Before image analysis of each characteristic can begin the software should calibrated using a calibration sheet from the developer Approximately 14 Gb per year Applications: Pea, pelargonium, impatiens, willow (leaves) Rape, mustard, fodder radish (leaves, flowers) Red clover (Cotyledon leaves, first leaves) Costs/savings: Depending on crop and object savings are higher than costs. remark: There is a need for a programmer with special knowledge how (at least part-time). It is possible to scan images at first and to make measurements later when there is more time therefore. Technical: 1. Hardware camera 1. Hardware processing 1. Hardware display flatbed scanner, digital camera HP workstation xw4400 19” monitor 2. Software recording 2. Software processing 2. Software display/print 3. Commercially available 4. Available to other members standard software (scanner, digital camera) SCIL-Image with self-programmed routines JAVA program to retrieve images from database Recording: Yes, from HP and Canon. Process: no. Display: partially No
5. Recording conditions 6. Volume of data 7. Other remarks Scanner: scan calibration circle (Ø12cm) to get the resolution we don’t use the color information digital camera: flash (ring flash) and background light 2011: 80 GB image data (14.300 files) Application : grain length Cost/savings: After arranging the seeds for the picture and taking of photographs, the analysis takes only a portion of time compared to manual measurements. Accurate costs and savings of IA has not been done, but roughly 10-20% of time is needed by using IA in these measurements. 1. Hardware camera 1. Hardware processing 1. Hardware display EOS 500D PC Dell screen 2. Software recording 2. Software processing 2. Software 3. Commercially available 4. Available to other members ¨ UTHSCSA ImageTool for Windows Version 3.0 Windows picture manager Capture: in camera shops, Process: downloadable from http://compdent.uthscsa.edu/dig/itdesc.html, Display: Windows Office 2012 5. Recording conditions 6. Volume of data 7. Other remarks Calibration: are kept close to each others in the picture to avoid distortion by the objective. The ruler is used for calibration of the scale in the picture before taking the analysis picture. Scale of 1 cm is included in each varietys pictures for calibration in the IT program 130 MB/each DUS year Application Crops: Carrot, Rape, Ornamentals, Seed/Grains various crops, Barley, Maize,
Oat, Pea. Characters: Width, Length, Area, perimeter, Curve length, Curve Width, Color, Mean distance, fineness of foliage, attack disease on leaves, how plant cover the ground, … 1. Hardware camera 1. Hardware processing 1. Hardware display different cameras (Nikon, Canon, Sony, HP, ) and scanners (Epson, HP) HP workstation HP workstation 2. Software recording 2. Software processing 2. Software display/print 3. Commercially available 4. Available to other members standard software (scanner, digital camera) Aim Software (Own development) + ImageJ (with own macros, plugins) Aim software Yes, from GEVES Yes 5. Recording conditions 6. Volume of data 7. Other remarks Application Rice: size Costs/savings: Cost of system (software+scanner) € 8000. Technical: 1. Hardware camera 1. Hardware processing 1. Hardware display Scanner Perfection V7000 PC Normal monitor 2. Software recording 2. Software processing 2. Software display/print 3. Commercially available 4. Available to other members Winseedle 2010a Winseedle 2010a Winseedle 2010a Yes, Regent instruments Commercially available 5. Recording conditions 6. Volume of data 7. Other remarks Winseedle with an optical scanner and a special lighting system that minimizes shadows The Applications:
Automated image measurements perform at least as well as manual measurements. Overall costs between manual (more recording) and imaged (more collection) measurements are about the same – but IA enables measured characters to be recorded which could not be done manually (e.g. leaflet area). Image library becomes available 1) For reference; 2) For data checking 3) for subsequently developed characters Other remark: We have downloaded a copy of the freely available software and have used it to investigate the possibility of assessing seed shape in large seeded crops but as yet we have not used this method in relation to variety testing. Technical: 1. Hardware camera 1. Hardware processing 1. Hardware display Canon EOS 450 DSLR (lab) Nikon D700 DSLR (studio) SASA network SASA network PCs (Dell) and printers (various 2. Software recording 2. Software processing 2. Software display/print 3. Commercially available 4. Available to other members Canon Imag-in’ Automatic Measurement Program (Biomathematics and Statistics Scotland) and ‘Portfolio’ v8.5 image database (Extensis). Portfolio camera and display yes, processing software no No 5. Recording conditions 6. Volume of data The Digital System is based on a digital camera using 2 11w lights @ 6000k which equate to natural light. The camera is suspended above a level surface. A reference object is incorporated into every picture. The IA program links with the studio Controller. The controller has special routines to handle camera aperture white noise etc. The linkage program has been specifically adapted by a consultant to allow menu driven options for each crop and to allow the entry of plot numbers. Once the controller has taken a picture the file is downloaded and re- opened by the IA program running in the background. The IA program will automatically analyze the picture and store the results in a data file. This allows the user to move any touching or irregular objects, thus equating to a live system. However the Controller can be used in isolation, storing the images to be batch processed at a later time. Once all plots have been photographed and analyzed via the batch process, thumbnail images can be examined and any outliers can be removed. 300 GB [Annex II follows]