WA PHL Biosafety Risk Assessment Procedure Risk Assessment Summary Table July 07, 2017 Protocol: Microbiology Geenius HIV 1/2 Supplemental Assay for Samples Mitigation Measures Specific Task/Activity Job Classifications Hazards Risks from Hazards Engineering Controls Administrative Controls Practices and Procedures Personal Protective Equipment Using a micropipettor, dispense 5 uL of serum/plasma to center of Sample + Buffer Well 1 of device. Microbiologist (1, 2, 3) HIV in Serum/Plasma Hepatitis B Virus in Serum/Plasma Hepatitis C Virus in Serum/Plasma Micropipettor Tips Micropipettor Percutaneous Exposure Mucosal Exposure Gastrointestinal Exposure Inhalational Exposure Percutaneous Exposure Mucosal Exposure Gastrointestinal Exposure Inhalational Exposure Percutaneous Exposure Mucosal Exposure Gastrointestinal Exposure Percutaneous Injury Ergonomic Injuries Plastic Biohazard Shield, Micropipettor Tips, Biological Waste Container on Benchtop, Negative Pressure HVAC Door Signage, Training Plan Assessment, Protocol Technical Competency Assessment, Hepatitis B Vaccination, Micropipettor Calibration Program Geenius HIV 1/2 Protocol, WA PHL Safety and Health Manual, WA PHL Biosafety Manual, WA PHL Exposure Control Plan, GMTs for micropipettors, GMTs for sharps and Closed-Front Laboratory Coat Safety Glasses Nitrile Gloves Closed Toe Shoes
Use diluent dropper bottle to add 2 drops (60 uL) of Buffer into Sample + Buffer Well 1. Microbiologist (1, 2, 3) HIV in Serum/Plasma Hepatitis B Virus in Serum/Plasma Hepatitis C Virus in Serum/Plasma Geenius HIV 1/2 Buffer Inhalational Exposure Percutaneous Exposure Mucosal Exposure Gastrointestinal Exposure Inhalational Exposure Percutaneous Exposure Mucosal Exposure Gastrointestinal Exposure Inhalational Exposure Percutaneous Exposure Mucosal Exposure Gastrointestinal Exposure Inhalational Exposure Skin Contact and Absorption Exposure Percutaneous Exposure Eye Contact and Absorption Exposure Gastrointestinal Exposure Allergic Plastic Biohazard Shield, Biological Waste Container on Benchtop, Negative Pressure HVAC Door Signage, Training Plan Assessment, Protocol Technical Competency Assessment, Hepatitis B Vaccination, Safety Data Sheets for Geenius HIV 1/2 Confirmatory Assay, Occupational Health Screening for Gentamicin Sulfate Allergy WA PHL Safety and Health Manual, WA PHL Biosafety Manual, WA PHL Exposure Control Plan, WA PHL Chemical Hygiene Plan, GMTs for liquid handling with biological hazards Closed-Front Laboratory Coat Safety Glasses Nitrile Gloves Closed Toe Shoes
Gastrointestinal Exposure Allergic Reaction to Gentamicin Sulfate Clean and decontaminate laboratory work surfaces. Microbiologist (1, 2, 3) Opti-Cide3 70% Ethanol Inhalational Exposure Skin Contact and Absorption Exposure Percutaneous Exposure Eye Contact and Absorption Exposure Gastrointestinal Exposure Fire and Explosion if Contacting Incompatible Chemicals Fire and Explosion if Contacting Heat Sources Fire and Explosion if Contacting Electrical Sources Inhalational Exposure Skin Contact and Absorption Exposure Percutaneous Exposure Biological Waste Container on Benchtop, Negative Pressure HVAC Door Signage, Training Plan Assessment, Protocol Technical Competency Assessment, Hepatitis B Vaccination, Safety Data Sheets for Disinfectants WA PHL Safety and Health Manual, WA PHL Biosafety Manual, WA PHL Exposure Control Plan, WA PHL Chemical Hygiene Plan Closed-Front Laboratory Coat Safety Glasses Nitrile Gloves Closed Toe Shoes
Emblems for Personal Protective Equipment Head Protection Hair Covering Face Shield Safety Glasses Goggles Hearing Protection Respirator Body Protection Laboratory Coat Laboratory Gown Scrubs Tyvek Suit Hand and Arm Protection Gloves Sleeve Cover Foot and Leg Protection Closed Toe Shoes Sturdy Shoes Shoe Cover Knee High Boot Cover Waist High Boot Cover Emblems for Hazards Biological Hazard Biosafety Level 2 (BSL-2) Biosafety Level 3 (BSL-3) Animal Biosafety Level 2 (ABSL-2) Animal Biosafety Level 3 (ABSL-3) Radiation Hazard Reproductive Hazard