respiration, and depressed or placement, righting, and pain reflexes. Animals at the three higher dosage levels also showed bloated abdomens. Death was immediately preceded by coma and a profuse bloody discharge from the eyes. Animals at the lower two dosage levels exhibited normal appearance and behaviour at 24 hours after dosage and thereafter. At 24 hours the remaining survivors appeared depressed and showed a bloody discharge around the eyes and laboured respiration, while those at 4.64 and 6.81 mL/kg levels also showed bloated abdomens, tachycardia, and depressed or absent placement and righting reflexes. These animals gradually recovered within an additional one to three days after which they appeared normal. Effects in Organs Gross autopsies performed upon the animals that died showed hyperaemic and inflated lungs, slight irritation of the small intestine and congested kidneys and adrenals. In addition the blood appeared to have a thin consistency and did not clot readily. No gross pathological findings were observed at autopsy of the surviving animals. Remarks - Results The notified chemical is of low toxicity via the oral route. Hazleton Laboratories (1957) 7.2. Acute toxicity - intravenous Notified chemical. Species/Strain Rat-Sprague Dawley Mouse- Swiss Webster Vehicle 0.9% sodium chloride solution Remarks - Method 14 day period Group Conc. (% v/v) Dose (mg/kg) Number and Sex of Animals LD50 (both sexes) Rat I 20 3160 3980 5010 6310 7940 10M/10F 10M/10F 10M/10F 10M/10F 10M/10F 5836 Rat II 40 3160 3980 5010 6310 7940 10M/10F 10M/10F 10M/10F 10M/10F 10M/10F 5369 Mouse I 20 4470 5620 7080 8910 10M/10F 10M/10F 10M/10F 10M/10F 10M/10F 6895 Mouse II 40 2820 3550 4470 5620 7080 10M/10F 10M/10F 10M/10F 10M/10F 10M/10F 5416
Remarks - Method Remarks - Results Tested on the eye mucosa of albino rabbits as an 80% and 60% w/v aqueous solution, the notified chemical did not cause irritation to the washed or unwashed eyes. All scores were zero. The notified chemical is non-irritating to the eye. Chemical industries Inc. (1964b) 7.5.3 Irritation - eye Notified chemical Primary irritation to the eye mucosa of the rabbit was tested and scored in accordance with the procedure outlined in AFDO, US (1959) Species/Strain Rabbit/New Zealand Number of Animals 4F/5M Observation Period 7 days Remarks - Method 0.1 mL instilled into eye- 6 unwashed, 3 washed for 2 seconds with 20 mL water. Concentration (%) Condition of eye in regard to wash after instillation Classification Kay and Calandra Code of Federal regulations No of eyes Positive/No. Tested 100 Unwashed Mildly irritating Negative 0/6 2 second wash Mildly irritating Negative 0/3 Remarks - Results The notified chemical, tested on the eye mucosa of albino rabbits as a 100% w/v aqueous solution, was classified as mildly irritating according to the interpretation of Kay and Calandra. The notified chemical is slightly irritating to the eye. Atlas Chemical industries Inc. (1968) 7.6.1 13-Week repeat dose oral toxicity Notified chemical OECD TG 408 Repeated Dose 90-Day Oral Toxicity Study in Rodents. Species/Strain Rat – Sprague Dawley. Route of Administration Oral – gavage/diet/drinking Exposure Information Total exposure days: 13 weeks Dose regimen: 7 days per week Post-exposure observation period: None Vehicle Water Remarks - Method