Acute oral toxicity (calcium/sodium salt) Rat LD50 >5.0 g/kg SEAC Toxicology Group, 1979b Acute oral toxicity (free acid) Mice LD50 4.1 g/kg SEAC Toxicology Group, 1976 Acute oral toxicity (free acid) Rats LD50 4.1 g/kg SEAC Toxicology Group, 1977a intraperitoneal toxicity (free acid) Mice LD50 0.30 g/kg SEAC Toxicology Group, 1977b percutaneous toxicity (free acid) Rabbits Non-toxic by skin absorption Younger Laboratories, 1968a Skin irritation – intradermal injection test (notified chemical) Rabbit Slight to moderate SEAC Toxicology 1980a Skin irritation – covered test (notified chemical) Rabbit Slight irritant SEAC Toxicology Group, 1980b Skin irritation (intradermal injection test; Komplex ) Rabbit Slight to moderate SEAC Toxicology Group, 1980c Skin irritation (intradermal injection test, Calcium analogue – Draise Product) Rabbit Slight to moderate SEAC Toxicology Group, 1980d Skin irritation – covered patch test (free acid) Rabbit Slight to moderate SEAC Toxicology Group, 1977c Skin irritation - intradermal injection test (free acid) Rabbits Slight to moderate SEAC Toxicology 1977d Eye irritation (notified chemical) Rabbit Slight irritant SEAC Toxicology Group, 1980e Eye irritation (free acid) Rabbit Moderate Younger Laboratories, 1968b Skin sensitisation (notified chemical) Guinea pig Non-sensitiser SEAC Toxicology Group, 1981a Skin sensitisation (notified chemical) Guinea pig Sensitiser SEAC Toxicology Group, 1983 Skin sensitisation (notified chemical) Guinea pig Weak sensitiser SEAC Toxicology Group, 1982 Skin sensitisation (calcium/sodium salt) Guinea pig Weak-sensitiser SEAC Toxicology Group, 1981b Skin sensitisation (calcium sodium salt) Guinea pig Non-sensitiser SEAC Toxicology Group, 1984 Skin sensitisation (free acid) Guinea pig Non-sensitiser SEAC Toxicology Group, 1977e Oral Toxicity Oral toxicity of the calcium/sodium salt (SEAC toxicology Group, 1979a) Species/strain: Mice (strain not specified) Number/sex of animals: 3/sex/group Observation period: 21 days
4 NE 0 NE 5 NE 0 NE 6 NE 0 0 Conjunctiva r c d r c d r C d 1 1 0 NE 0 0 0 0 0 0 2 1 0 NE 1 0 0 0 0 0 3 1 1 NE 1 0 0 0 0 0 4 0 0 NE 0 0 0 NE NE 5 1 0 NE 0 0 0 NE NE 6 1 0 NE 1 0 0 0 0 0 1 see Attachment 1 for Draize scales O = opacity, a = area, r = redness, c = chemosis, d = discharge, NE = Not estimated Result: The notified chemical was slightly irritating to the eyes of rabbits. Eye irritation with free acid (Younger Laboratories, 1968b) Species/strain: New Zealand White rabbit Number/sex of animals: 3 (2 males, 1 female) Observation period: 1 to 7 days after treatment Method of administration: 100 mg of finely ground free acid powder were placed into the conjunctival sac of the right eye of each rabbit. The eyes were examined for signs of irritation after 1, 24, 48, 72, 120 and 168 hours. The treated eyes were rinsed with warm isotonic saline 24 hours after treatment later. After the reading, fluorescein (concentration not stated) was instilled to detect corneal damage. Test method: Not stated Comment: Treated eyes were graded according to the severity of erythema, oedema, discharge, corneal opacity and iridial reactions on an arbitrary scale of 0 to 110. Moderate discharge and erythema, mild oedema and mild corneal cloudiness developed in all treated eyes 1 hour. Iris clarity improved overnight and discharge had nearly ceased within 3 days. A slight degree of redness and oedema remained after 7 days. Result: The free acid was considered to be a moderate eye irritant in rabbits under conditions of the test. Skin Sensitisation Skin Sensitisation test with the notified chemical (SEAC Toxicology Group, 1981b) Species/strain: Guinea pig/ Dunkin-Hartley Number of animals: 10 (6 males, 4 females) Induction procedure: