Table 3. 2 Summary of 18S rRNA sequence analyses of oligotrophic fungi cultured from phosphate spoils, desert-vegetated soil and desert non-vegetated soil. Samples Closest matches Identification Sequence Identity NBCl (Accession number) 1- soil Cochliobolus lunatus 99.0 % JQ724488.1 soil Penicillium daleae 90.0 % Aj850133.1 3-Phosphate spoil Mucor sp 92% Jx160050.1 4-Desert Non vegetated soil Aspergillus oryzae 85 % Fj654482.1
Mucor Sp. Pikovskaya 10.9 3.7 2.3 A number of reports have detailed the ability of bacteria to solubilize insoluble inorganic phosphate compounds, notably tricalcium phosphate, dicalcium phosphate, hydroxyapatite, and rock phosphate (Altomare et al, 1999; Baijpai et al., 1971; Goldstein, 1986; Nahes et al.1996; Nannipieri et al. 2011; Venkateswarlu et al. 1984; Wu et al. 2009; Yandaw et al., 2011). Bacterial are generally involved in this process. The bacteria which are capable of solubilizing phosphates include species of Pseudomonas, Bacillus, Rhizobium, Burkholderia, Achromobacter, Agrobacterium, Microccocus, Aereobacter, Flavobacterium and Erwinia (Hilda and Frega, 1999). Considerable populations of phosphate-solubilizing bacteria occur in soil, most notably in the rhizosphere, including both aerobic and anaerobic species, with the latter, not surprisingly dominating submerged soils (Goldstein, 1986). Phosphate solubilizers can
∞ 4°C 5-2-4. Analysis of amplified products by gel electrophoresis The PCR product (10 μl) was with 2 μl of Blue 6x loading dye and run on a 2% agarose gel. In addition, 6 μl of 1 Kb Hyper ladder loading in the gel to confirm the correct sized of product. The PCR test sample (10µl) positive and negative controls were then placed in 2% agarose gel wells and (2µl) of 6× loading dye was placed in wells. The gel was immersed in TAE buffer 1× with 2.5µl ethidium bromide. 6 µl of Hyper Ladder 200 bp (Bioline, UK) was used in this experiment. Finally, the samples were subjected to electrophoresis for 40 minutes at 80V. The amplified products were finally visualized on the gel and digital images were taken using “UVitec” attached to a digital camera. 5-3. Results and Discussion 5.3. The occurrence or absence of Mycoplasma in different environmental samples. Samples Results Phosphate spoil Negative Desert soil Negative Desert non-vegetated soil Negative
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