Table 1. Sites sampled during 2008-2011. Darter occurrence (presence or absence) at each site is also shown. Asterisks indicate sites randomly selected for distribution analysis. “Y” and “N” indicate fin clips were or were not taken, respectively. Site ID Site Description Watershed State Northing Easting Visit 1 Visit 2 Visit 3 Visit 4 Visit 5 Visit 6 Variegate darters Fin Clips LF001 Levisa Fork, 2nd riffle upstream of saline diffuser pipe Levisa Fork VA 402104 4126636 6/11/10 6/30/11 present N LF002 Levisa Fork, 1st riffle upstream of saline diffuser pipe Levisa Fork VA 401965 4126665 6/11/10 6/30/11 present N LF003 Levisa Fork, 1st riffle downstream of saline diffuser pipe Levisa Fork VA 401568 4126751 5/26/10 7/15/10 7/16/10 10/21/10 6/30/11 present (except visit 5) N LF004 Levisa Fork, 2nd riffle downstream of saline diffuser pipe, just upstream of Wellmore Hollow Road Levisa Fork VA 401392 4126770 6/11/10 6/30/11 present N LF005 Levisa Fork upstream of Vansant, VA Levisa Fork VA 405928 4121671 7/29/08 8/6/08 present N LF006 Levisa Fork behind Keen Mountain, VA post office Levisa Fork VA 412705 4117709 7/29/08 8/11/08 absent N LF007 Levisa Fork downstream of Wellmore Hollow Road Levisa Fork VA 400012 4128146 8/6/08 8/13/08 present Y LF008 Levisa Fork at County Road 604 near Harman Junction, VA Levisa Fork VA 397558 4129566 8/6/08 8/13/08 present Y LF009 Bull Creek at Bull Creek Road Levisa Fork VA 396690 4130073 7/29/08 8/13/08 present Y
RF001 Russell Fork at Bridge Street in Haysi, VA Russell Fork VA 385185 4118360 8/4/08 8/12/08 absent N RF002 McClure River off Highway 83 near Clinchco, VA Russell Fork VA 381435 4116549 8/4/08 8/12/08 absent N RF003 Frying Pan Creek off County Road 625 Russell Fork VA 390642 4109569 8/4/08 8/12/08 absent N RF006 Russell Fork off Highway 80 in Davenport, VA* Russell Fork VA 399255 4106347 6/4/09 7/6/09 7/29/09 absent N RF007 Indian Creek off State Route 602 upstream of Duty, VA* Russell Fork VA 398631 4104212 6/4/09 7/6/09 absent N RF022 Indian Creek at Highway 602 downstream of Duty, VA* Russell Fork VA 397024 4106257 8/11/10 absent N RF008 McClure River off Highway 83, ~ 1.25 mi downstream of intersection with State Route 840* Russell Fork VA 378157 4110831 6/22/09 7/1/09 7/29/09 absent N RF009 Russell Fork off State Route 605* Russell Fork VA 389138 4114501 6/22/09 7/13/09 7/29/09 absent N RF010 Russell Prater Fork off State Route 613* Russell Fork VA 383972 4120030 6/29/09 7/13/09 absent N RF011 Russell Prater Fork off SR 611* Russell Fork VA 383034 4122343 6/29/09 7/17/09 absent N RF012 Pound River near Norland, VA* Russell Fork VA 364648 4114195 7/1/09 7/22/09 absent N RF013 Cranes Nest River off Highway 72* Russell Fork VA 367698 4104359 6/24/10 absent N
RF014 Lick Creek off Lick Creek Road, just downstream of Wilson Drive near Aily, VA* Russell Fork VA 385070 4107006 6/24/10 absent N RF015 Russell Fork off Russell Fork River Road near Jeremiah Road* Russell Fork VA 381581 4113908 6/23/10 absent N RF016 Russell Fork just upstream of wastewater treatment plant* Russell Fork VA 384872 4118902 5/28/10 absent N RF017 Russell Fork downstream of confluence with Pound River* Russell Fork VA 382252 4123308 5/28/10 absent N RF018 McClure Creek ~ 1/2 mi downstream of Highway 83/63 split to Clintwood/McClure, VA* Russell Fork VA 378075 4109894 7/15/10 absent N RF019 McClure River, off Highway 83* Russell Fork VA 380890 4115322 6/22/11 7/14/11 absent N RF020 Birchfield Creek, off Route 632 and Mountainview Road* Russell Fork VA 365119 4103224 6/22/11 7/6/11 7/13/11 absent N RF021 Cranes Nest River, off Cranes Nest River Campground Road* Russell Fork VA 373850 4111965 6/22/11 7/13/11 absent N TF001 Knox Creek ~50 m upstream of State Route 697 and State Route 642* Tug Fork VA 406573 4145048 6/10/09 7/2/09 absent N TF002 Guesses Fork off Guesses Fork Road* Tug Fork VA 411213 4144109 6/10/09 7/2/09 absent N
Table 7. Year, site location, population assignment, site number (#), UTM coordinates and number of variegate darters used in genetic analyses. Sites without site numbers (-) were not part of habitat or population sampling, only genetic tissue sampling. Year Site Population Site # Northing Easting Number analyzed 2008 Levisa Fork 500 m downstream of Highway 83 Levisa Upstream LF014 402202 4121326 19 2008 Levisa Fork upstream of Vansant, VA Levisa Upstream LF005 405928 4121671 10 2008 Levisa Fork at County Road 604 near Harman Junction, VA Levisa Upstream LF008 397558 4129566 33 2008 Dismal Creek off County Road 638 Levisa Upstream LF012 409258 4122680 2 2009 Slate Creek in Grundy, VA near confluence with Levisa Fork Levisa Upstream LF017 403040 4126205 6 2009 Levisa Fork upstream of Fishtrap Lake Wildlife Management Area Levisa Upstream - 388898 4139640 40 2009 Dismal Creek upstream of Pilgrims Knob, VA Levisa Upstream LF021 422704 4121993 3 2009 Levisa Fork near Pikeville, KY Levisa Downstream - 365071 4147436 10 2009 Levisa Fork just downstream from Fishtrap Lake dam Levisa Downstream - 374798 4143371 3 2009 Levisa Fork just downstream from Fishtrap Lake dam Levisa Downstream - 373669 4141493 36 2009 Levisa Fork downstream of Fishtrap Lake near Shelbiana, KY Levisa Downstream - 367855 4143566 18 2009 Russell Fork in Regina, KY Levisa Downstream - 374992 4136419 43 2009 Beaver Creek Levisa Downstream - 347600 4163604 1 2009 Dry Fork downstream of Crane Creek confluence Tug TF008 430701 4141155 31 2009 Tug Fork at Horse Creek confluence Tug TF006 423650 4147187 17 2009 Tug Fork near Merrytown, WV Tug TF009 440862 4147500 1 2010 Allegheny River downstream of Union Street bridge, Olean, NY Allegheny - 712617 4660802 20