Keywords and/or search strategy used: alternative, refinement, replacement, reduction and Were alternatives found? Yes No The potential alternatives and why they are or are not applicable: Search 3 Database Searched: Period covered by search: Date of search: Keywords and/or search strategy used: alternative, refinement, replacement, reduction and Were alternatives found? Yes No The potential alternatives and why they are or are not applicable: Description of Experimental Design and Animal Procedures Please do not respond to any questions with the statement “industry practice” as the community and nonscientist members of the committee will not be familiar with industry practice and will not have the information necessary to vote. Please use layperson’s terms to the extent possible. A diagram or chart may be helpful to explain complex designs. Yes No Will major survival surgery be performed on any animal prior to being placed on this study? If Yes, please explain below what was done to the animal(s): Briefly explain the experimental design and specify the treatments (including nutritional manipulations) and the number of animals per treatment group. This description should allow the IACUC to understand the experimental course of an animal from its entry into the experiment to the endpoint of the study. Details on surgery should be included in Section II, I of this form. If appropriate, you may reference IACUC approved Standard Operating Procedures. Will the diet fed to the animals meet National Research Council or commonly accepted nutritional requirements for the species? Yes No If No is selected, provide justification below: Will the food or fluid be restricted? (excluding pre-surgical fast) Yes* No *If Yes, provide the below information: Please provide justification below:
Indicate calorie or nutrient restrictions or excess: Indicate the length of time the animals will be on the experimental diet: Will this project involve injections, immunizations, inoculations or drugs? Please include any paralytic agents that may be used. Please do not include any other injections, inoculations or drugs that will be used for surgery as this will be captured in the surgery section. Yes No No- Go to Question 4. Yes- Fill out the reset of Question 3. Substance Dose (mg/ kg) Frequency and Route of Anatomic Site Volume (administered per site and total administered at one time) Is this pharmaceutical grade? * Yes No Yes No Yes No Yes No Yes No *Attach an Appendix A “Administration of non-pharmaceutical grade compounds” for each substance that is not a pharmaceutical grade compound. I will log all controlled substances per regulations and DEA Law. Yes No I am not using controlled substances Will the animals be anesthetized or sedated during the procedure? Yes* No *If Yes, list the anesthetic agent or sedative, including dosage and route of administration.
Will this study involve the administration of anything with the potential to be lethal or cause significant symptomatology? Yes No If Yes, please describe: Will paralytic agents be used? Yes No If Yes, please describe how administration will be maintained and how pain will be assessed: Paralytic agents cannot be used without anesthetic agents. Will paralytic agents be used during surgery? Yes No If Yes, please describe frequency of observation, and how pain will be assessed. Identify the responsible individual(s). Note: paralytic agent cannot be used without an anesthetic agent. Paralytic neuromuscular blocking agents include succinyl choline, tubocuraine chloride, atracuium beslyate, gallamine triethiodide, vencuronium bromide, and pancuronium bromide, pipecuronium, and doxacurium. Will this project involve administration of substances to animals that have not been approved for marketing by the FDA/USDA-APHIS? Yes No If Yes, attach Appendix A. Will this project involve blood or other fluid collection? Yes No If Yes, please describe below including fluid type, volume, frequency, withdrawal sites, and methodology. For wild caught animals indicate whether the method used is standard procedures or complete details if it is not standard procedure and include an estimate of the expected mortality for each sampling method. Will animals be restrained in any way? Yes No No- Go on to Question 6. Yes- Complete all parts of Question 5.
Please describe postoperative care; include when the sutures will be removed: Describe any adverse consequences the animals may be expected to experience as a result of the surgical procedure(s) described above: List what care will be provided for animals suffering these consequences and who will be responsible for providing this care: Section III: Environmental Health and Safety If this project will involve the use of anesthetic gas, please complete 1-3. List the anesthetic gases to be used during this protocol: Describe how the gas will be administered: Check the protective equipment for gas scavenging that will be used: Filter system—if checked, is the filter an active or passive capture? Fume hood None Other—please explain: Cages needs to be changed in fume hood, changing table, laminar flow hood or portable laminar flow table. If this project involves your research staff or you changing bedding or cages, please complete below: Indicate the type of respiratory protection used by personnel while changing cages or bedding. N95 respirator, or other type of respirator list type: Surgical mask, etc. – list type: Cage Changing Station Biosafety cabinet