Cacopsylla melanoneura Forster C. crataegi Low-number populations were present. The regime of chemical protection greatly affects the population density of psyllids. AP - Apple entomological net morphological Cacopsylla picta Forster Low-number populations were present. The regime of chemical protection greatly affects the population density of psyllids. AP Apple entomological net morphological Cacopsylla pyri Linnaeus It was present in populations mainly in the intensive production pear orchards PD, BN Number of tested individuals is not enough to determine the infection rate. Few insects tested positive. Molecular (PCR/RFLP) Pear entomological net morphological Cacopsylla pyricola Forster It was present in populations only in the extensive pear orchards. BN Molecular (PCR/RFLP) Pear entomological net morphological
COST Action FA0807 WG 2/Task 2 QUESTIONNAIRE I Title: Integrated Management of Phytoplasma Epidemics in Different Crop Systems Working Group 2: Epidemiology and Vector Ecology Task 2: MONITORING THE PRESENCE OF PHYTOPLASMA DISEASES AND THEIR PUTATIVE VECTORS IN DEFINED REGIONS THROUGH EUROPE Your address Name Institution country/region email / telephone Nicolas SAUVION INRA, FRANCE +33 499 62 48 41
I Title: Integrated Management of Phytoplasma Epidemics in Different Crop Systems Working Group 2: Epidemiology and Vector Ecology Task 2: MONITORING THE PRESENCE OF PHYTOPLASMA DISEASES AND THEIR PUTATIVE VECTORS IN DEFINED REGIONS THROUGH Your address Name Institution country/region email / telephone Barbara Jarausch RLP AgroScience, AlPlanta_Institute for Plant Research, Neustadt a.d.Weinstrasse, Rhineland-Palatinate, Germany ++49-6321-6711308 Presence of phytoplasma diseases detected in your country/region
Region/s Phytoplasma/s Strain/s Phytoplasma disease Incidence Host/s Symptoms Detection method/s Nahe 16SrXII-A; Stolbur tuf A tuf B Bois noir Low, locally high (20-40%) Grapevine, C.arvensis, U. dioica Potato Grapevine Yellow Stunting, Yellowing No clear symptoms Typical symptoms of Potato stolbur PCR-RFLP Pfalz 16SrXII-A; Stolbur 16SrV; PGY; AldY tuf A ((tuf B)) type A,B,C Bois noir Palatinate GY Alder Yellows Low, locally high Low <3% Very high Grapevine, C.arvensis, U. dioica Grapevine Alnus glutinosa Grapevine Yellow Stunting, Yellowing No clear symptoms Grapevine Yellows No clear symptoms PCR-RFLP Rheingau 16SrXII-A; Stolbur tuf A tuf B Bois noir Low Grapevine, C.arvensis, U. dioica Grapevine Yellow Stunting, Yellowing No clear symptoms PCR-RFLP Rheinhessen 16SrXII-A; Stolbur tuf A ((tuf B)) Bois noir Low Grapevine, C.arvensis, U. dioica Grapevine Yellow Stunting, Yellowing No clear symptoms PCR-RFLP Württemberg 16SrXII-A; Stolbur tuf A ((tuf B)) Bois noir Low, locally very high Grapevine, C.arvensis, U. dioica Grapevine Yellow Stunting, Yellowing No clear symptoms PCR-RFLP
COST Action FA0807 WG 2/Task 2 QUESTIONNAIRE I Title: Integrated Management of Phytoplasma Epidemics in Different Crop Systems Working Group 2: Epidemiology and Vector Ecology Task 2: MONITORING THE PRESENCE OF PHYTOPLASMA DISEASES AND THEIR PUTATIVE VECTORS IN DEFINED REGIONS THROUGH EUROPE Your address Name Institution country/region email / telephone Ibolya EMBER Maria KOLBER Sandor SULE Genlogs Biodiagnostics Ltd. Hungary Genlogs Biodiagnostics Ltd. Plant Protection Institute , 0036-706205130 , 0036-706205124 0036-204935112
COST Action FA0807 WG 2/Task 2 QUESTIONNAIRE I Title: Integrated Management of Phytoplasma Epidemics in Different Crop Systems Working Group 2: Epidemiology and Vector Ecology Task 2: MONITORING THE PRESENCE OF PHYTOPLASMA DISEASES AND THEIR PUTATIVE VECTORS IN DEFINED REGIONS THROUGH Your address Name Institution country/region email / telephone Prof. Piero Attilio Bianco Di.Pro.Ve. piero.bianco@unimi.it Rosemarie Tedeschi DIVAPRA rosemarie.tedeschi@unito.it ITALY Presence of phytoplasma diseases detected in your country/region Region/s Phytoplasma/s Strain/s Phytoplasma disease Incidence Host/s Symptoms Detection method/s Lombardy Ca. P. mali AT-1 Apple proliferation 10 to 70 % apples Witches-broom unusually long stipules smaller fruits Nested PCR (F1(X)/R1(X)) Lombardy Veneto Stolbur Tuf-I Tuf-II Bois noir 10 to 50% Grapevine, nettles, bindweeds Leafroll, yellowing and reddering, no production Real time PCR Lombardy Veneto Flavescence doree FD-C FD-D Flavescence dorée 5 to 30 % grapevine Leafroll, yellowing and reddering, no production Real time PCR Piedmont Flavescence dorée FD-C FD-D Flavescence dorée 1-50% of the plants within the same vineyard Grapevine Leaf yellowing or redness, leaf curling, canes remaining green (no lignification) Nested PCR (F1(V)/R1(V)) M1/B6 + RFLP Stolbur Tuf-A Tuf-B Bois noir < 1% oft he plants within the same vineyard Grapevine Same as FD Nested PCR (F1(I)/R1(I)) r tuf/f tuf + RFLP stol1H10 F2/R2 +
RFLP Aosta Valley Flavescence dorée FD-C FD-D Flavescence doree Less than 1 plant per vineyard Grapevine Leaf yellowing or redness, leaf curling, canes remaining green (no lignification) Real Time PCR (FAY/REY) Nested PCR (P1/P7 + F1/V1731) + RFLP (F1(V)/R1(V)) M1/B6 + RFLP Stolbur Tuf-A Tuf-B Bois noir 0 - 50% oft he plants within the same vineyard Grapevine Same as FD Nested PCR (F1(I)/R1(I)) r tuf/f tuf + RFLP stol1H10 F2/R2 + RFLP Pidmont Valle d’Aosta Ca. P. mali AT-1 AP-15 AT-2 Apple proliferation 5 to 70% Apples Hawthorn Witches-broom unusually long stipules smaller fruits Nested PCR (F1(X)/R1(X)) (fO1/rO1) + RFLP Pidmont Valle d’Aosta Ca. P. pyri Pear Decline 10 to 70% pear Leaf reddening, smaller fruits Nested PCR (fO1/rO1) + RFLP Piemonte Ca. P. prunorum European Stone Fruit Yellows 5 to 30% (plum) 2-4 % (apricot) Plum, apricot Chlorotic leaf roll, early foliation Nested PCR (fO1/rO1) + RFLP Piedmont Liguria Ca. P. asteris 16SrXI-B Chrysantemum yellow Chrysantemum spp., Argyranthemum frutescens, Tagetes spp., etc… Leaf yellowing and chlorosys, phyllody Nested PCR F1/R1(I) + RFLP
Phytoplasma vectors (putative/acknowledged) identified in your country/region Vectors (aknowledged) Vectors (putative) Vector host\s Phytoplasma/s No. of insects positive/examined Detection method/s Collection method/s Identification method/s Macrosteles laevis Rib Solanum tuberosum 16SrIII – X-disease group 5/16 Nested PCR mowing with entomological net RFLP Aphrodes bicinctus Schrk. Trifolium hybridum 16SrI – Aster yellow group 3/4 The same The same The same Cicadula quadrinotata F. Lotus corniculatus Mixture 1/4 The same The same The same Empoasca pteridis Dhlb. L. corniculatus 16SrI – Aster yellow group 1/3 The same The same The same Euscelis plebejus Fall. T. hybridum 16SrI – Aster yellow group 8/11 The same The same The same T. pratense 7/18 The same The same The same Medicago sativa 1/1 The same The same The same L. corniculatus 2/2 The same The same The same Philaenus spumarius L. L. corniculatus 16SrI – Aster yellow group 1/6 The same The same The same Sonronius binotatus J.Shlb. T. hybridum 16SrI – Aster yellow group 1/28 The same The same The same Publications: Unpublished COST Action FA0807 WG 2/Task 2 QUESTIONNAIRE I
Clematis vitalba (Krnjajić, 2009) common PCR, RFLP Apple proliferation (16SrX) ’Ca. mali’ ’Ca. prunorum’ (16SrX-B) piry’ (16SrX-C) Apple proliferation apple Šutić (1995) pear (Vojvodić and Grbić, 1969) apple ((Duduk et al., 2005b) plum, apricot (Tanasković and Carraro, 2003), peach (Duduk et al., 2005b) pear (Duduk et al., 2005a) common common common common common common Visual inspection Visual inspection PCR, RFLP PCR, RFLP PCR, RFLP PCR, RFLP
Stolbur (16SrXII) solani’ (16SrXII-A) Corn reddening Bois noir 85% Solanacea (Martinović and Bjegović, 1950) Solanacea, Convolvulus arvensis (Aleksić et al., 1967;1969; Šutić, 1995) Sonchus oleraceus, C. arvensis (Duduk, 2006b) corn, (Duduk and Bertaccini, 2006) Triticum aestivum, Sorghum halepense (Jovic et al., 2009) peach (Duduk et al., 2005b) grapevine (Duduk et al., 2004a) chrysanthemum (Duduk et al., 2005b, 2006a) common common common common common common common common Visual inspection Visual inspection, transmittion PCR, RFLP PCR, RFLP PCR, RFLP PCR, RFLP PCR, RFLP PCR, RFLP
Lee I.M., Martini M., Marcon C., Zhu S.F. (2004): Classification of phytoplasma strains in the elm yellows group (16SrV) and proposal of ‘Candidatus Phytoplasma ulmi’ for the phytoplasma associated with elm yellows. International Journal of Systematics and Evolutionary Microbiology 54, 337–47. Magud B., Toševski I. (2004): Scaphoideus titanus Ball. (Homoptera, Cicadellidae) nova štetočina u Srbiji.- Biljni lekar, Novi Sad, 32(5), 348-352. Radonjić, S., Hrnčić, S., Jović J., Cvrković, T., Krstić, O., Krnjajić, S., Toševski, I. (2009): Occurrence and Distribution of Grapevine Yellows Caused by Stolbur Phytoplasma in Montenegro. Journal of Phytopatholology 157, 682-685. Schneider B, Gibb KS, Seemüller E. (1997) Sequence and RFLP analysis of the elongation factor Tu gene used in differentiation and classification of phytoplasmas. Microbiology 143, 3381-3389.
Publications: COST Action FA0807 WG 2/Task 2 QUESTIONNAIRE I Title: Integrated Management of Phytoplasma Epidemics in Different Crop Systems Working Group 2: Epidemiology and Vector Ecology Task 2: MONITORING THE PRESENCE OF PHYTOPLASMA DISEASES AND THEIR PUTATIVE VECTORS IN DEFINED REGIONS THROUGH EUROPE
Your address Name Institution country/region email / telephone J.Th.J. Verhoeven M. de Kock M. Verbeek PPS The Netherlands WUR-PPO The Netherlands WUR-PRI The Netherlands / / / Presence of phytoplasma diseases detected in your country/region Phytoplasma vectors (putative/acknowledged) identified in your country/region