Inches Pounds Force Gauge 2 Scale that will measure masses and record its data 2.53 in. L 1.32 in. W 6.74in. H 1 lbs per Total = 2(1) = 2 lbs. Tough Box 1 Holds gears and motor in place 5 in. L 1.57 in. W 5 in. H 2 lbs Motor 1 Turns axel and runs experiment 2.5in diameter with 4.34 in. long body 1.5 lbs 20in. bar of 10 series jaluminum (80-20) 4 Frame of experiment 20 in. L 1 in. W 1 in. H .85lbs per bar Total = 4(.85) = 3.4 lbs. 18in. bar of 10 series aluminum (80-20) 12 Frame of experiment 18 in. L by 1in. W by 1in. H .765 lbs per bar Total = 12 (.765) = 9.18 lbs Four hole angled joining plate 28 Connects aluminum bars. Length 2 in. Height 2 in. Width 1 in. Approx. .0625lbs per Total = 28(.0625) = 1.75 lbs. Hex head screws ¼-20 3/8” 112 Holds angled plates to the aluminum ¼-20 3/8” Approx. .004lbs per Total = 112 (.004) =
bars. .448 lbs. Servo tester with Jaguar motor controller w/ four AA battery pack 1 Used to control power output to the motor. N/A .75 lbs 12 V DC Power Supply 1 Used to Power the motor 7 in. L 3 in. W 6 in. H 14 lbs Testing Weights 2 These will be used to compare the weights of a known to an unknown object. Less than 1 in3 .0022lbs n/a Surge Protected Power Strip 1 Used to protect against surges and run experiment 16 in. L 2 in. W 1 in. H .8 lbs Hex Screw Nuts 112 Used to lock bolts in place N/A Approx. .004lbs per Total = 112 (.004) = .448 lbs. Total Approx. 37 lbs Hardware Class
Table 3. Observation of Fly Behavior in Altered Gravity7 Ethanol Exposure 3 Ground 1 Flight 2 Zero Time Crawl on the sides of vials, negative geotaxis4 Crawl on sides of vials, negative geotaxis 50% ST50 5 More time on bottom of vial, some incapacitated, random twitching, difficulty climbing above the base Some flies begin to drop off sides of vial ST50 ½ intoxicated, nearly all crawling on or near bottom, rapid twitching and wing flutters Floaters and spinners in microgravity, free fall in hypergravity, some hooked to bottom6 2 times ST50 All on bottom, little crawling, and twitching All floaters, spinners, or clinging to bottom 1 Flies exposed to Ethanol on the Ground 2 Flies exposed to Ethanol on the Zero-G Flight 3 Exposed to 500μl Ethanol; times are approximations 4 Crawling up to the top of the vial 5 ST50 is the standard time taken for 50% of a vial of flies to pass out; 50% ST50 is an estimate of approximately half that time 6 Floaters are totally passed out, spinners have vibrating wings which cause them to wildly spin in microgravity; floaters free fall to the bottom of the vial in hypergravity; some flies stay hooked to the bottom of the vial with their claws 7Behaviors were similar for wild type and adh flies DISCUSSION Our results indicate that enzyme activity is affected in altered gravity. Both the wild type and adh strain metabolized significantly more ethanol on the flight than on the ground. Comparisons of adh and wild type patterns of metabolism clearly show differences which indicates effects on the alcohol dehydrogenase enzyme. However, the fact that metabolism in the adh strain was greatly impacted by altered gravity indicates that other genes are involved, and suggests that altered gravity may affect many or all enzymes. This experiment had a large sample size with minimal variation; however, we would like to repeat this experiment to see if the results are consistent. We would also like to test other Drosophila mutants that affect alcohol metabolism to see if the affect varies. The purpose of the behavior experiments was to see if flies exposed to ethanol in altered gravity would have altered sensitivity to ethanol. The ST50 test was utilized as it is a simple assay on the
NanoRacks Payloads Ratings per NanoLabModule Maximum Mass per CU.……………….…1000g Maximum Power per CU.……………....2 Watts Maximum Voltage.…………………….….5 VDC Maximum Current per CU.………….….400 mA Maximum Cooling per CU.………….….2 Watts Data.……………...................USB Connectivity Crew Time.……………..Negotiated as needed Delivery Timeframe:……….L-6 to Late Access Return Mass.……………….Soyuz limit to 1 kg Transport Method……….Cargo Transfer Bags APPENDIX G FAST FACTS
Synergism Vibration Vibration Tool High Noise Level Source Metal Fatigue Casation Flow or Jet Vibration Supersonic Miscellaneous Contamination Lubricity Violent Odor Training Hypoxia Structural Failure JSC Safety and Health Handbook References See Sec. 2.4 (for effective date April 16, 2008 pp. 79-87) http://jsc-aircraft-ops.jsc.nasa.gov/Reduced_Gravity/docs/JPR1700.1RevJ.pdf TOOL REQUIREMENTS Additional Tools that will be at Ellington Field ALL tools brought to Ellington Field must be approved by RGO. Special Tools Required on the Aircraft
Consequence Class Likelihood Estimate A B C D E I 1 1 2 3 4 II 1 2 3 4 5 III 2 3 4 5 6 IV 3 4 5 6 7 If the RAC is… Then the risk is… 1 Unacceptable – All operations shall cease immediately until the hazard is corrected, or until temporary controls are in place and permanent controls are in work. A safety or health professional shall stay at the scene at least until temporary controls are in place. RAC 1 hazards have the highest priority for hazard controls. 2 Undesirable – All operations shall cease immediately until the hazard is corrected or until temporary controls are in place and permanent controls are in work. RAC 2 hazards are next in priority after RAC 1 hazards for control. Program Manager (directorate level), Organizational Director, or equivalent management is authorized to accept the risk with adequate justification 3 Acceptable with controls – Division Chief or equivalent management is authorized to accept the risk with adequate justification 4–7 Acceptable with controls – Branch Chief or equivalent management is authorized to accept the risk with adequate justification (I will help you fill in this table. Every hazard you list in your TEDP must be listed below and how you controlled it to be safe. I have listed the Sharp Corners for an example.) HAZARD CAUSE EFFECT Sev/Prob RAC CONTROLS VERIFICATION DISPOSITION Sev Prob RAC Sharp corners and edges Lexan box has sharp corners and edges Hitting edge or corner may cause a slight injury III/D 5 Edges and corners of Lexan box will be padded or taped and is inside glove box Padding and taping is in place Controlled III/E 6