Time Monday to Friday Saturday and Bank Holidays Sunday 08:00 – 20:00 Normal service* On call service† 08:00 – 12:30 Restricted service‡ 12:30 – 20:00 On call service† 20:00 – 08:00 On call service† On call service† On call service† * Normal routine service – Contact ext 3000. NB: Single handed Biomedical Scientist between 17:30 and 20:00. † Out-of-Hours service. Please bleep Chemistry on 5116, Haematology & Blood Transfusion on 3066 ‡ Restricted service for Priority Requests only, which must be received in the laboratory by 11.00hrs to allow for processing Microbiology Day Normal hours of Service* Restricted Service‡ On Call Service† Weekdays 09.00-17.30 17.30-09.00 Saturdays 09:00-12.30 12.30-09.00 Sundays and Public holidays 09.00-12.30 12.30-09.00 * Normal routine service – Contact ext 5645. † Out-of-Hours service. On Call Biomedical Scientist: will be contactable by bleep (3078) when they are on site but if not answering the bleep contact can be made via switchboard who will know of their whereabouts. On Call Consultant Microbiologist: contact can be made via switchboard. ‡ Restricted service for Priority Requests only, which must be received in the laboratory by 11.00hrs to allow for processing Histopathology Time Monday to Friday Saturday and Bank Holidays Sunday 08:45 – 17:00 Normal service* No service No service 17:15 – 08:45 No service No service No service * Please note that urgent specimens must reach the department before 16:45 unless by prior arrangement with a consultant Histopathologist. Phlebotomy Wards Time Monday to Friday Saturday and Bank Holidays Sunday 08:00 – 12:00 Normal service* Restricted service‡ Restricted service‡
CK ≥ 1000 IU/L All First episode Cortisol ≤ 50 mmol/L All All Unless Dexamethasone test Creatinine ≥ 354 μmol/L All Except renal (dialysis) unit First episode No need to phone if there is evidence that a similar high conc. has been previously phoned. ≥ 200 μmol/L < 16 yr First episode CRP ≥ 300 mg/L GP patients First episode Digoxin ≥ 2.5 µg/L First episode Ethanol ≥ 4000 mg/L All All ≥ 2000 mg/L <18 yrs All Glucose ≤ 2.5 mmol/L & ≥ 20 mmol/L NOT if a known diabetic All ≥ 30 mmol/L if diabetic All ≥ 15 mmol/L <16 yr old All Test Value Group Episode Comment HbA1c ≥120 mmol/mol GP only First episode Phone results to GP in core hours only. OOH leave on phone list - Phoned by BMS to GP on the following working day Iron > 55µmol/L All All Lithium ≥ 1.5 mmol/L All All Magnesium ≤ 0.4 mmol/L All First episode unless drops further Paracetamol Any results All All unless undetectable Phenytoin ≥ 25 mg/L All All Phosphate ≤ 0.3 mmol/L First episode unless drops further Potassium ≥ 6.5 mmol/L ≤ 2.5 mmol/L All First episode Protein ≥100 g/L All First episode Sodium ≤ 120 mmol/L & ≥ 155 mmol/L All First episode <110 mmol/L All All ≤ 130 mmo/L, <16 yrs First episode ≥ 175 mmol/L All First episode Request an Urgent repeat UE and random sample for
sample and repeat 3 hours later Total Protein 1 x gold 24 hr 3 hr See Report g/L 5 days IN HOUSE Triglycerides 1 x gold 24 hr 3 hr See Report mmol/l 5 days IN HOUSE FASTING Urea 1 x gold 24 hr 3 hr See Report Age and Sex Related mmol/l 5 days IN HOUSE DIET RELATED Uric Acid 1 x gold 24 hr 3 hr See Report FEMALE mmol/l 5 days IN HOUSE Uric Acid 1 x gold 24 hr 3 hr See Report MALE mmol/l 5 days IN HOUSE Vitamin A 1 x green 2 weeks N/A See Report ADULT RANGE µmol/L N/A LEEDS MORLEY Protect from light 2 weeks N/A See Report PAED RANGE µmol/L N/A LEEDS MORLEY Vitamin E 1 x green 2 weeks N/A See Report ADULT RANGE µmol/L N/A LEEDS MORLEY 2 weeks N/A See Report PAED RANGE µmol/L N/A LEEDS MORLEY Vitamin B12 1 x gold 48 hr 5 hr See Report ng/L 5 days IN HOUSE Vitamin D 1 x gold 5 days N/A See Report nmol/L N/A IN HOUSE Zinc 1x dark blue 120 hr N/A See Report Sex related µmol/l 5 days IN HOUSE Urine Biochemistry Container Turnaround Times Variations Units Retention Time Test performed Special Conditions Routine Urgent 5HIAA 24hrs + 50ml 30%HCl 2-3 Weeks N/A See Report µmol/24hr N/A HULL Amino Acid(quantitative) Plain, random 1-2 Weeks N/A See Report SEE REPORT N/A LEEDS ST.JAMES’ Amino Acid (screen) Plain, random 1 week 2-3 days See Report SEE REPORT N/A LEEDS ST. JAMES Alcohol Plain, random 24 hr 3 hr See Report mg/l 5 days IN HOUSE Bence Jones protein Plain 120hr N/A See Report SEE REPORT 5 days IN HOUSE Early morning urine Calcium 24hrs + 50ml 48 hr 5 hr See Report FEMALE mmol/24hr 5 days IN HOUSE
HAEMATOLOGY, IMMUNOLOGY & BLOOD TRANSFUSION Laboratory Staff Contact Details Name Job Title Tel.No / Bleep / Pager Email Mr Jeff Walker Blood Sciences Manager 01423 555842 Miss Gayle Sugden Transfusion Manager 01423 553070 Mrs Rebecca Parish Adv BMS - Haematology 01423 553068 Contacts for Advice and Clinical Interpretation Name Job Title Area of responsibility Tel.No / Bleep / Pager Email Dr Claire Hall Head of Department General Haematology 01423 553062 Dr Tara Balasubramanian Consultant Blood Transfusion 01423 552271 Tharani.Balasubramaniam@hdft.nhs.uk Dr Emma Harris Consultant Haemostasis 01423 552218 Dr Marketa Wilson Consultant General Haematology 01423 553067 Marketa.Wilson@hdft.nhs.uk Mrs Rose Gill Transfusion Practitioner Clinical Transfusion 01423 555628 Dr Sinisa Savic Consultant Immunology Network 0113 2065567 Dr Philip Wood Consultant General Immunology 0113 2067256 philipwood1@nhs.net
Haematology, Immunology & Blood Transfusion Investigations The following list of available tests is not exclusive and other tests may be available on request. Investigation Specimen required Turnaround times Routine Urgent Reference Range Comments, Special precautions. Specimen retention time Performed at Adrenal Abs 1 x gold 14 days n/a Positive or Negative n/a LGI Activated Partial Thromboplastin time (APTT) 1 x blue 4 hours 1 hour 24 - 30 Seconds 24 hours HDH APTT Ratio – Heparin monitoring 1 x blue 4 hours 1 hour (Therapeutic range 1.5 – 2.5) Not for monitoring LMWH 24 hours HDH Acetylcholine receptor Antibodies 1 x gold 14 days n/a < 5 x 10-10 molar: Neg 5 - 50 x 10-10 molar : + 50–500 x 10-10 molar ++ > 500 x 10-10 molar : +++ n/a ORH Anticardiolipin Abs 1 x gold 14 days n/a 0 – 19.9 GPL Units / ml n/a LGI Anti DNA – now part of ANA Screen (Leeds) 1 x gold 14 days n/a TBC Request ANA Screen (Leeds) on ICE N/A LGI Anti-neutrophil cytoplasmic Abs (Screen plus MPO, 1 x gold 14 days (2 days) Positive or Negative for screen, numerical report for ANCA MPO and PR3 Request ANCA Screen (Leeds) on ICE N/A LGI
PR3 and pattern if applicable) (Ref range TBC) Antinuclear Abs 1 x gold 2 weeks 4 weeks (Titre) n/a Pattern if appropriate Request ANA Screen (Leeds) on ICE N/A LGI Ascitic Fluid WBC/Differential 1 x purple 4 hour 1 hour Differential not possible on WBC counts under 1.0 x 109/L 24 – 48 hours HDH Blood Film Consultant advice required 1 day 1 hour Descriptive report issued Consultant advice required 7 days HDH Blood Group and Screen / Cross match 1 x pink (6 ml) 1 day 1 hour Descriptive report issued for Group and Screen 14 days HDH Bone marrow aspirate & trephine Consultant advice required 1 week 1 week Descriptive report issued By prior arrangement only n/a HMDS Cyclic Citrullinated Peptide(CCP) Abs. 1 x gold 48 hours n/a 0<16 U/mL 5 Days LGI
Coagulation Screen (PT & APTT) 1 x blue 4 hours 1 hour See PT & APTT 24 hours HDH D-dimers 1 x blue 4 hours 1 hour 0 – 0.55 mg/L FEU < 0.50 has a Negative predictive value for VTE 24 hours HDH Direct Coombs Test 1 x pink (6 ml) or purple (4 ml) 1 day 1 hour Negative / Positive (IgG +/- C3d) 14 days HDH Endomysial Abs. 1 x gold 14 days n/a Negative / Positive IgA class Only tested on Positive TTGs NA LGI EPO 1 X gold 4 weeks 3 weeks By prior arrangement only n/a HMDS Erythrocyte Sedimentation rate (ESR) 1 x Black top sodium citrate 48 hours 90 minutes Females: 0-13mm/hr Males: 0-8mm/hr Samples must reach the blood science laboratory within 4 hours of collection 48 hours HDH Extractable Nuclear Antigens – Request ANA Screen (Leeds) 1 x gold 4 weeks n/a Now included in ANA Screen (Leeds) on ICE N/A LGI Fetal Rh(D) Screening 1 x pink 14 days n/a Positive/Negative/ Inconclusive Fully labelled with at least 3 points of ID Sign and date sample. EDD from NA IBGRL
Thrombocytopenia Screen (HIT-type II) 1 x blue arrangement only HLA B27 2 x purple (2 x 4 ml) 2 Weeks n/a Ag Negative / Ag Positive Ensure fully labelled with at least 3 points of ID n/a NBS HLA for Narcolepsy 2 x purple (2 x 4 ml) 2 Weeks n/a Negative / Positive Ensure fully labelled with at least 3 points of ID n/a NBS INR (Warfarin monitoring) 1 x blue 4 hours 1 hour Therapeutic Range Normal therapy 2.0 – 3.0 High Thrombotic risk 3.0 – 4.5 24 hours HDH Intrinsic Factor Abs. 1 x gold 14 days NA Positive / Negative / Equivocal TBC n/a SJUH JAK 2 Contact laboratory (3068) 6 weeks 5 weeks See report By prior arrangement only n/a HMDS Liver Antibody Screen 1 x gold 2 weeks n/a Negative / Positive For AMA, SMA, LKM and GPC antibodies N/A LGI Malarial Parasites 1 x purple 4 hours 2 hours Negative / Positive (plus species identification) Record country visited & any prophylaxis on HDH
request form Pemphigus Abs. 1 x gold 14 days n/a Negative / Positive (+ titre) n/a LGI Pemphigoid Abs. 1 x gold 14 days n/a Negative / Positive (+titre) n/a LGI Plasma Viscosity 1 x purple 72 hours 5 hours 1.5 – 1.72 mPa.s SJUH Platelet Abs. Contact laboratory (3068) 7 – 10 days 3 days Descriptive report issued By prior arrangement only n/a NBS Pleural Fluid WBC/Differential 1 x purple 4 hours 1 hour Differential not possible on WBC counts under 1.0x109/L HDH Prothrombin time (PT) 1 x blue 4 hours 1 hour 8.0 – 12.0 Seconds (For Warfarin monitoring request INR) 24 hours HDH Paraneoplastic Neurological Syndrome antibodies (PNSS) 1 x gold 14 days (screen) 28 days (profile) n/a Positive, Negative or Equivocal Full Profile will be performed on Positive and equivocal screens n/a LGI Reticulocytes 1 x purple 4 hours 1 hour 20–80 x 109 / l 24 hours HDH
Aspergillus antibodies Gold 10 N/A LGI* Advice provided by reference laboratory on final report where applicable Atypical pneumonia screen (acute) Gold 14 2-3 mths LGI* Advice provided by reference laboratory on final report where applicable Atypical pneumonia screen (convalescent) Gold 14 N/A LGI* Advice provided by reference laboratory on final report where applicable Avian antibodies Gold 10 N/A LGI* Advice provided by reference laboratory on final report where applicable Bordetella pertussis antibodies Gold 14 N/A COL* Advice provided by reference laboratory on final report where applicable Bordetella pertussis PCR Pernasal swab / NPA 4 N/A COL* Advice provided by reference laboratory on final report where applicable Borrelia antibodies Gold 5 4 wks In-house This assay has not been established with heat-inactivated, haemolysed, lipaemic or icteric samples. Borrelia antibodies confirmation Gold 21 N/A PPHE* Advice provided by reference laboratory on final report where applicable Brucella antibodies Gold 14 N/A BRU* Advice provided by reference laboratory on final report where applicable Campylobacter antibodies Gold 10 N/A RPH* Advice provided by reference laboratory on final report where applicable Candida antigen Gold 10 N/A LGI* Advice provided by reference laboratory on final report where applicable Chlamydia antibodies Gold 7 N/A LGI* Advice provided by reference laboratory on final report where applicable
Taenia antibodies Gold 14 N/A HTD* Advice provided by reference laboratory on final report where applicable Tetanus antibodies Gold 14 N/A SJH* Advice provided by reference laboratory on final report where applicable Toxocara antibodies Gold 14 N/A HTD* Advice provided by reference laboratory on final report where applicable Toxoplasma antibodies Gold 5 4 wks In-house This assay has not been established with haemolysed, lipaemic or icteric, neonatal samples or cord blood. Toxoplasma confirmation Gold 10 N/A SHS* Advice provided by reference laboratory on final report where applicable Toxoplasma PCR Purple 7 N/A SHS* Advice provided by reference laboratory on final report where applicable Varicella zoster virus (VZV) PCR Purple 5 N/A LGI* Advice provided by reference laboratory on final report where applicable VZV IgG Gold 3 4 wks In house This assay has not been established with heat-activated, haemolysed, lipaemic or icteric samples. VZV IgM Gold 10 N/A LGI* Advice provided by reference laboratory on final report where applicable Weil’s disease See Leptospira antibodies