Dose (mg/kg): 200 a) terminal kill no gross abnormalities 500 no abnormalities Dose (mg/kg): 500 b) decedents within 4 hours of treatment yellowish in the intestinal tract 710 1 000 haemorrhagic stomach and reddish mucoid material in the intestinal orange mucoid material in the intestinal tract 2 000 reddish mucoid material in the intestinal tract and one animal also had haemorrhagic stomach Dose (mg/kg): 1 000 c) decedents within 24 hours of treatment haemorrhagic stomach and orange mucoid material in the intestinal tract Test method: according to the Environmental Protection Agency Health Effects Testing Guidelines (40 CFR, Section 163-81-1 Federal Register), which is similar to OECD test guideline (Organisation for Economic Cooperation and Development, 1987c) LD50: males: 590 mg/kg females: 540 mg/kg Result: the notified chemical was of low oral toxicity in rats Dermal Toxicity (Costello, 1984a) Species/strain: rabbit/New Zealand White Number/sex of animals: 5/sex/dose Observation period: 14 days Method of administration: 200 or 2 000 mg/kg of test substance held under occlusive dressing; after 24 hours, excess material was wiped away :
metabolic activation; no significant increases in revertant colonies for any of the bacterial strains at any dose with or without metabolic activation; Test method: according to OECD guidelines (Organisation for Economic Cooperation and Development, 1983b), (Organisation for Economic Cooperation and Development, 1983a) Result: the notified chemical was considered not to be mutagenic in the bacterial strains tested with and without metabolic activation Micronucleus Assay in the Bone Marrow Cells of the Mouse not conducted. Overall Assessment of Toxicological Data The studies were conducted on a reaction mixture known as M-1999, containing the notified chemical. The notifier made claims for variation of the Schedule data requirements (acute inhalation studies, skin sensitisation, repeat dose toxicity and in vivo germ cell induction and in vitro chromosome damage) on the basis that the notified chemical is never isolated from the reaction mixture and therefore the pure substance is not available for testing. This claim for variation was accepted. M-1999 exhibited low acute oral toxicity in rats: the LD50 for males was 590 mg/kg and for females 540 mg/kg. In an acute dermal toxicity test, M-1999 was moderately toxic to rabbits with an LD50 in the range of 200 to 2 000 mg/kg. M-1999 is an eye irritant, with corneal and conjunctival effects present in rabbits at 21 days. Necrotic skin was visible after a 4-hour treatment in the skin corrosivity test and severe oedema and/or well-defined erythema-pallor persisted for seven days in the skin irritation study. M-1999 was not mutagenic in Salmonella typhimurium and Escherichia coli strains with or without metabolic activation. M-1999 is toxic via the oral and dermal route, irritating to the eye, and corrosive to skin. M- 1999 is not mutagenic to bacteria. Therefore, on the basis of the toxicological data provided, M-1999 would be classified as a hazardous substance (Toxic) in accordance with the NOHSC Approved Criteria (ref). The appropriate risk phrases are R24 – Toxic in contact with skin, R22 – Harmful if swallowed, R34 – Causes burns, and R36 – Irritating to eyes.