Dissociation data for the notified chemical, which is a substituted phenol, were not supplied. Substituted phenols are weakly acidic (Morrison & Boyd 1976). Despite the notifier claiming that the chemical will not dissociate, it is possible that some dissociation will occur in the environmental pH range of 4 to 9. 4. PURITY OF THE CHEMICAL Degree of Purity: Not applicable, the chemical is a UVCB Hazardous Impurities: Chemical name: Calcium hydroxide CAS No.: 1305-62-0 Weight percentage: 0.5% Toxic properties: Irritant Chemical name: Phenol, (tetrapropenyl) derivatives manufacture of distillation residues CAS No.: 220794-73-0 Weight percentage: 0.1% Toxic properties: Skin irritant Chemical name: Phenol, (tetrapropenyl) derivatives CAS No.: 74499-35-7 Weight percentage: 1% Toxic properties: Skin irritant Chemical name: Phenol, C20-30 alkyl derivatives CAS No.: - Weight percentage: 1.5% Toxic properties: Skin irritant Non-hazardous Impurities (> 1% by weight): Details claimed as exempt information. Additives/Adjuvants: 20% diluent oil, hydrotreated or solvent refined, heavy paraffinic distillates. Exact details of solvent are claimed as exempt.
Test substance: OLOA 229 Species/strain: Rabbit/New Zealand White Number/sex of animals: 4 males Observation period: 14 days Method of administration: A single, 24-hour occlusive application of 5 000 mg/kg of test substance to abraded (2 rabbits) and intact skin (2 rabbits). Test method: OECD TG 402 Mortality: Nil Clinical observations: No signs of toxicity observed Morphological findings: No gross abnormalities detected LD50: > 5000 mg/kg Result: OLOA 229 was of low dermal toxicity to the rabbit. 9.1.3.1 Skin Irritation (Cavalli MS 1970) Test substance: OLOA 229 Species/strain: Rabbit/New Zealand White Number/sex of animals: 6 males Observation period: 3 days; observations done at 24 and 72 hours. Method of administration: A single, 24-hour occlusive application of 0.5 mL of test substance to abraded and intact skin on each rabbit. Test method: Not stated but similar to OECD TG 404 Dermal scores for intact skin: Time Animal # treatment (days) 1 2 3 4 5 6 Erythema 1 a2 1 2 2 1 1
28 day, oral; (WIL Research Laboratories Inc 1993) 0, 50, 200,1000 NOEL=50 (subchronic) NOAEL 1000 (neurotoxicity) NOEL=50 (reproductive) Clinical signs, reduced bodyweight gains and changes in organ weights. Highest dose tested. Reduced bodyweights, and decrease in live litter size. 28 day, dermal; (SOCAL 1986) 0, 20, 100, 250 NOEL 250 (dermal) Highest dose tested. Developmental, oral; (CEHC 1990) 0, 50, 300, 1000 NOEL=300 (parental) NOEL=50 (developmental) Reduced bodyweight gains and food consumption. Malformation. Developmental, oral; (WIL Research Laboratories 1994) 0, 50, 300, 1000 NOEL=300 (parental). NOEL=300 (developmental) Reduced bodyweights and food consumption. Malformation. Oral, two-generation; (WIL Research Laboratories 1995b) 0, 50, 300, 1000 NOAEL=50 (parental) NOAEL=300 (reproductive) NOAEL=50 (developmental) Mortality, clinical signs, reduced bodyweight gains, and changes of organ weights. Lower fertility indices. Lower pup survival rates, live litter sizes and pup bodyweights. Oral reproductive & developmental; (WIL Research Laboratories 0, 50, 250 (in finished oil) NOEL 250 (reproductive & developmental) Highest dose tested 9.3 Genotoxicity 9.3.1 Salmonella typhimurium Reverse Mutation Assay (Chevron Environmental Health Center Inc 1986b) Test Substance: XA-294C (OLOA 319) Strains: Salmonella typhimurium: TA100, TA1535, TA98, TA1537 Auxillary metabolic activation system: Liver S9 fraction from rats induced with Aroclor 1254