Comment: In the E. coli reversion test, no increase in the number of revertant colonies was observed at any concentration, compared with the negative control. In the DNA repair test, no zone of inhibition was observed for the test substance. The positive controls behaved appropriately in both tests. Result: The notified chemical was non mutagenic under the conditions of the test. 9.3.3 Tradescantia Stamen Hair System (Mičieta, 1990) Strains: Tradescantia 4430 Concentration range: 0.2% (w/v) test substance in DMSO 0.2% (v/v), Tween 80 (v/v) 0.2% in water. Test method: The tester plant species is heterozygous at a flower colour locus. A blue phenotype is a product of a dominant blue allele, and a pink phenotype is controlled by a recessive pink allele unmasked by mutation or deletion of the blue allele. For each treatment, entire inflorescens are immersed in test solution, washed and planted in Hoagland’s nutrient solution. For each treatment, 10 flowers/3000 stamen hairs were scored daily between days 4 and 12 after commencement of treatment. Comment: The test substance was insoluble in water and of low solubility in organic solvents. Result: The notified chemical was non mutagenic under the conditions of the test. 9.3.4 In vivo Cytogenetic Test – Test (Murín, 1990) Strain: sativa L. cv. Fatima Concentration range: 0.2, 0.1 or 0.05% (w/v) test substance in 0.4% (v/v) acetone, Tween 80 (one drop) in water. Test method: Seeds were immersed in test substance solutions for 24 hours and then germinated in petri dishes on filter paper wetted by similar solutions. After 72 hours, root tip samples were evaluated for structural and numerical chromosome mutations. Comment: The test substance was insoluble in water and of low solubility in organic solvents. No chromosome mutations were observed for any concentration of test substance. The positive and negative controls behaved appropriately.
Doses: 0, 200, 400 and 800 mg/kg body weight Method of administration: Gavage Test method: OECD TG 474 Comment: No significant increase in the incidence of micronucleated cells was observed at any dose level. Result: The notified chemical was non clastogenic under the conditions of the test. 9.3.8 Dominant Lethal Test (Rosival and Szokolayová, Species/strain: Rats, Wistar Number and sex of animals: 20 females, 10 males Doses: 16.6mg/kg body weight (to Method of administration: Orally by diet Test method: OECD TG 478 Comment: This is a poorly reported study with no information on control procedures. No mortality in female rats was reported prior to autopsy. Increases in post-implant (0.7%) and total dominant lethality (1.9%) of foetuses were reported compared to controls but these were not deemed statistically significant. Result: The notified chemical appears non mutagenic under the conditions of this test. 9.3.9 Teratogenicity (Rosival and Szokolayová, 1985f) Species/strain: Mice, ICR strain Number and sex of animals: 4 groups, 20 females per group Doses: 0, 100, 200, 300 (or 400) mg/kg/day, days 6-15 of pregnancy. Method of administration: Gavage Test method: OECD TG 414 Comment: This is a poorly reported study with contradictory information on doses. Additive and dislocated ossification