Comments on Chemical Identity The notified chemical is never isolated, but is formed together with the butyl analogue, with the resulting mixture used in the formulation of brake and clutch fluids. The notifier has provided the composition of two representative brake fluids. One of these (named Shell Brake Fluid Dot 4) contains the notified chemical. The butyl ester analogue of the notified chemical is the subject of a separate new chemical notification (NA/657) and is also contained in Shell Brake Fluid Dot 4. PHYSICAL AND CHEMICAL PROPERTIES Appearance at 20°C, 101.3 kPa: clear liquid Boiling Point: > 300C at 102.5 kPa Density: 1 050 – 1 080 kg/m3 at 20 C Vapour Pressure: 0.2 kPa (estimated – see notes below) Water Solubility: completely soluble - see notes below Partition Co-efficient (n-octanol/water): log Pow < 1 (estimated) - see notes below Hydrolysis as Function of pH: no data available - see below Adsorption/Desorption: no data provided - see notes below Dissociation Constant: no data provided - see notes below Flash Point: > 144°C (estimated from similar products) Flammability Limits: not determined Autoignition Temperature: > 320°C Explosive Properties: none Reactivity/Stability: hydrolysis of the ester groups in the presence of water Comments on Physico-Chemical Properties
Mortality: none Morphological findings: none Test method: OECD TG 402 LD50: > 2 000 mg/kg Result: the test substance was of low acute dermal toxicity in rats Inhalation Toxicity Not done. Skin Irritation (Gardner, 1990; Gardner, 1992) Shell Brake Fluid Dot 4 Species/strain: rabbit/New Zealand Number/sex of animals: 3/sex Observation period: 72 hours Method of administration: 0.5 mL of undiluted test substance semi- occlusive dressing for 4 hours Test method: EEC Method B4 Result: the notified chemical was not a skin irritant in rabbits; no erythema or oedema was observed in any animal at any time point Shell Brake Fluid Dot 4 Super Species/strain: rabbit/New Zealand White Number/sex of animals: 3/sex Observation period: 72 hours Method of administration: 0.5 mL of undiluted test substance under semi- occlusive dressing for 4 hours Test method: OECD TG 404 Result: the notified chemical was not a skin irritant in
Dose/Study duration:: 0, 25, 150 or 1 000 mg/kg/day for 28 days Clinical observations: no clinical signs and no bodyweight changes or changes in food consumption in any dose group Clinical chemistry/Haematology no significant observations Macroscopic findings: no macroscopic findings and no organ weight changes Histopathology: the livers of all males and some females at the top dose exhibited slight centrilobular hypertrophy Test method: not specified Result: the NOEL was 150 mg/kg/day on the basis of liver centrilobular hypertrophy at 1 000 mg/kg/day Genotoxicity Salmonella typhimurium Reverse Mutation Assay with Shell Brake Fluid Dot 4 (Brooks & Wiggins, 1992) Shell Brake Fluid Dot 4 Strains: Salmonella typhimurium strains TA 1535, TA 1537, TA 1538, TA 98, TA 100 and Escherichia coli WP2 uvrA(pKM101) Concentration range: 31.1 – 5 000 g/plate Test method: Maron and Ames (Maron & Ames, 1983): metabolic activation was provided by Aroclor 1254-induced rat liver S9 fraction; positive control compounds to check that the strains exhibited the appropriate responses were benzo(a)pyrene, potassium dichromate, neutral red, sodium azide, 2- nitrofluorene, 2-aminoanthracene and 9- aminoacridine