カエル卵抽出液
Proc Natl Acad Sci USA 108: 17331-17336. PMID 21908712.
↑ Pomerening JR, Kim SY, Ferrell JE Jr (2005). “Systems-level dissection of the cell-cycle oscillator: bypassing positive feedback produces damped oscillations”. Cell 122: 565-578. PMID 16122424.
↑ Shimamoto Y, Maeda YT, Ishiwata S, Libchaber AJ, Kapoor TM (2011). “Insights into the micromechanical properties of the metaphase spindle”. Cell 145: 767-778. PMID 21703450.
↑ Hara Y, Merten CA (2015). “Dynein-based accumulation of membranes regulates nuclear expansion in Xenopus laevis egg extracts”. Dev Cell 33: 562-575. PMID 26004509.
通常の卵抽出液を超遠心分画し(100,000~200,000g)、膜成分とリボソームを除いた(可溶性タンパク質が含まれる)分画を超遠心分画と呼ぶ。HSSでは細胞周期に応じたクロマチン構造変化を部分的に再現できるが、核形成や翻訳は起こらない。タンパク質の精製に適している。
“Cell cycle extracts”. Methods Cell Biol. 36: 581-605. PMID 1839804.
1 2 Murray AW, Kirschner MW (1989). “Cyclin synthesis drives the early embryonic cell cycle”. Nature 339: 275-280. PMID 2566917.
↑ Walter J, Sun L, Newport J (1998). “Regulated chromosomal DNA replication in the absence of a nucleus”. Mol. Cell 1: 519-529. PMID 9660936.
↑ Räschle M, Knipscheer P, Enoiu M, Angelov T, Sun J, Griffith JD, Ellenberger TE, Schärer OD, Walter JC (2008). “Mechanism of replication-coupled DNA interstrand crosslink repair”. Cell 134: 969-980. PMID 18805090.
↑ Lohka MJ, Hayes MK, Maller JL (1988). “Purification of maturation-promoting factor, an intracellular regulator of early mitotic events”.