アデノ随伴ウイルス
. This discovery allowed for new production methods of recombinant AAV, which do not require adenoviral co-infection of the AAV-producing cells. In the absence of helper virus or genotoxic factors, AAV DNA can either integrate into the host genome or persist in episomal form. In the former case integration is mediated by Rep78 and Rep68 proteins and requires the presence of ITRs flanking the region being integrated. In mice, the AAV genome has been observed persisting for long periods of time in quiescent tissues, such as skeletal muscles, in episomal form (a circular head-to-tail conformation).
ゲノムの左末端にはp5及びp19プロモーターと、イントロンを含むmRNA領域が存在する。したがってスプライシングの結果分子量の異なるRep78,Rep68,Rep52,Rep40の4つの転写産物が合成される。 Rep78とRep68はITRのヘアピン構造に結合する。またRepタンパクはすべてATPと結びついてヘリカーゼ活性を発揮する。またp40プロモーターによって転写がアップレギュレーションされ、p5,p19プロモーターによって転写がダウンレギュレーションされる
“Latent Adeno-Associated Virus Infection Elicits Humoral but Not Cell-Mediated Immune Responses in a Nonhuman Primate Model”. Journal of Virology 73 (10): 8549–58. PMC 112875. PMID 10482608. http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=10482608.
↑ Ponnazhagan S, Mukherjee P, Yoder MC, et al. (April 1997). “Adeno-associated virus 2-mediated gene transfer in vivo: organ-tropism and expression of transduced sequences in mice”. Gene 190 (1): 203–10. doi:10.1016/S0378-1119(96)00576-8. PMID 9185868.
↑ McCarty, D M; Monahan, P E; Samulski, R J (2001). “Self-complementary recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently of DNA synthesis”. Gene Therapy 8 (16): 1248–54. doi:10.1038/sj.gt.3301514. PMID 11509958.
↑ Rogers GL (Jan 2014).
gov/pmc/articles/PMC114572/.
↑ Nony P, Chadeuf G, Tessier J, Moullier P, Salvetti A (January 2003). “Evidence for packaging of rep-cap sequences into adeno-associated virus (AAV) type 2 capsids in the absence of inverted terminal repeats: a model for generation of rep-positive AAV particles”. Journal of Virology 77 (1): 776–81. doi:10.1128/JVI.77.1.776-781.2003. PMC 140600. PMID 12477885. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC140600/.
↑ Philpott NJ, Giraud-Wali C, Dupuis C, et al. (June 2002). “Efficient integration of recombinant adeno-associated virus DNA vectors requires a p5-rep sequence in cis”. Journal of Virology 76 (11): 5411–21. doi:10.1128/JVI.76.11.5411-5421.2002. PMC 137060. PMID 11991970. https://www.ncbi.