caption: the computerworld slide to presenter's manual r, january 2004 rapid drug suceptility tests
exif: {"Image DocumentName": "Rapid Drug Susceptibility Tests", "Image ImageDescription": "ISTC TB Training Modules Line-probe assays. Identifies M.tb and genetic mutations associated with INH and RIF resistance. Can be used directly on sputum specimens, results within 1-2 days. With the rising rates of MDR and XDR, the need for rapid and accurate identification of drug resistance is significant. Tests currently in use or under investigation include: Line probe assays: DNA from M.tb directly from sputum samples can be amplified and identified. Probes can both identify M.tb and test for drug resistance based on the presence of genetic mutations associated with specific drug resistance (isoniazid and rifampicin). Has a high sensitivity (>=97%) and specificity (>=99%) for rifampicin resistance. Overall accuracy for detection of MDR equally high at 99%, and retained when rifampicin resistance alone was used as a marker for MDR. The WHO has endorsed their use for both direct smear-positive sputum and culture specimens. - Image shows examples of GenoType MTDBRplus strips (Hain Lifescience, Nehren, Germany). [Resource: WHO Policy Statement, Molecular line probe assays for rapid screening of patients at risk of multi-drug resistant tuberculosis (MDR-TB), ] Other rapid drug susceptibility tests \u2013 examples listed here are in use or under investigation in a more limited setting, most requiring more technical laboratory capabilities. [Image credit: Rapid Molecular Screening for Multidrug-Resistant Tuberculosis in a High-Volume, Barnard et al. Am. J. Respir. Crit. Care Med 2008; 177: ]  GenoType MTDBRplus strips. (Hain Lifescience) Molecular beacons. Bacteriophage-based assays.  Barnard et al. Am. J. Respir. Crit. Care Med 2008; 177:", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}