Orphan metabolic activities <p>A method that combines local structure of a metabolic network with phylogenetic profiles is described and used to assign genes to orphan metabolic activities in yeast and <it>Escherichia coli</it>.</p> Homology-based methods fail to assign genes to many metabolic activities present in sequenced organisms. To suggest genes for these orphan activities we developed a novel method that efficiently combines local structure of a metabolic network with phylogenetic profiles. We validated our method using known metabolic genes in Saccharomyces cerevisiae and Escherichia coli. We show that our method should be easily transferable to other organisms, and that it is robust to errors in incomplete metabolic networks.
Chen and Vitkup REF presented a method that uses local structure of a metabolic network combining with phylogenetic profiles to suggest candidate genes for enzymes without corresponding genes.
Predicting genes for orphan metabolic activities using phylogenetic profiles
Genome Biology
Genome Biology
Biology Medicine