We attempted to develop a device for measuring electrical corneal resistance (CR) using corneal contact lens electrodes to quantitatively evaluate corneal injury in vivo. In the present study, full-thickness detachment of the corneal epithelium was induced by n-heptanol, and the feasibility of the quantitative evaluation of this injury by corneal contact lens electrodes was evaluated in vivo. Methods: The central area of an albino rabbit's cornea was exposed to a filter paper preimmersed in n-heptanol for 1 minute to induce injury of the corneal epithelium. After induction of injury, the electrical CR was measured and the percentage of CR (%CR) was calculated. Fluorescein solution (3 µL) was applied to the wound/affected area of the corneal epithelium for photography with a slit-lamp biomicroscope. The wound/affected area was measured using an image analysis system. The correlation between the %CR and the wound/affected area was analyzed. Results: As the size of the wound/affected area of the corneal epithelium increased, the %CR decreased after corneal epithelium detachment. Thus, a close correlation was found between the area of corneal epithelium detachment and the %CR. The corneal resistance device that we developed was capable of quantitatively evaluating n-heptanol-induced full-thickness injuries of the corneal epithelium.
Fukuda and Sasaki REF attempted to quantify corneal epithelium injury by measuring electrical corneal resistance with some success.
Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo
Clinical Ophthalmology (Auckland, N.Z.)
Clinical Ophthalmology (Auckland, N.Z.)
Medicine