score, we applied it to 465 patients with early chronic phase chronic myeloid leukemia treated with standard-dose imatinib (n ‫؍‬ 71), high-dose imatinib (n ‫؍‬ 208), or secondgeneration tyrosine kinase inhibitors (n ‫؍‬ 186), and assessed its ability to predict event-free survival (EFS), transformationfree survival (TFS), and overall survival (OS). The median follow-up was 69 months. The overall complete cytogenetic response and major molecular response rates were 92% and 85%, respectively. The 3-year EFS, TFS, and OS rates were 86%, 95%, and 97%, respectively. Of the 465 patients, 427 (92%) were in low EUTOS score category. There was no difference in the major molecular response, TFS, EFS, and OS rates between patients with low and high EUTOS score, overall and within specific therapies. In conclusion, 8% of patients with chronic phase chronic myeloid leukemia treated at our institution are in the high EUTOS score; in this population, the EUTOS score was not predictive for outcome. (Blood. 2012;119(19):4524-4526) The introduction of the tyrosine kinase inhibitors (TKIs), which suppress the molecular processes driving chronic myeloid leukemia (CML), has revolutionized the management and prognosis in CML. 1 Imatinib therapy induced high rates of complete cytogenetic response (CCyR) and major molecular response (MMR), and improved survival in CML. [2] [3] [4] [5] [6] Second-generation TKIs (dasatinib, nilotinib) are more potent BCR-ABL inhibitors with demonstrated efficacy in patients resistant to or intolerant of imatinib. 7, 8 Dasatinib and nilotinib were first approved for patients resistant to or intolerant of prior imatinib therapy, are active against most BCR-ABL mutations with the exception of T315I, and have well-established safety profiles. 9,10 Single-arm phase 2 studies [11] [12] [13] suggested, and phase 3 randomized trials later confirmed, that dasatinib and nilotinib were superior to imatinib, inducing faster and higher rates of CCyR and molecular responses. Therefore, both drugs were granted FDA approval as initial therapy for patients with newly diagnosed CML in chronic phase (CML-CP). 14, 15 Until recently, the prognosis of patients with CML treated with TKI was based on scores developed in the chemotherapy and interferon era. 16, 17 The European LeukemiaNet has developed a new scoring system (European Treatment and Outcome Study [EUTOS] score) using data from 2060 patients with newly diagnosed CML-CP treated with imatinib-based regimens. The EUTOS score was reported to have superior prognostic power compared with the Sokal score. 18 The EUTOS score using the percentage of basophils and spleen size divided patients in 2 groups of low-and high-risk patients with significant correlations with the achievement of an 18-month CCyR and progression-free survival. The aims of this study were to validate the EUTOS score in an independent cohort of patients with early CML-CP referred to our institution and treated with TKIs, and to assess its ability to predict event-free survival (EFS), transformation-free survival (TFS), and overall survival (OS). A total of 465 consecutive patients with newly diagnosed CML-CP were treated with imatinib 400 mg daily (n ϭ 71), imatinib 800 mg daily (n ϭ 208), and second-generation TKIs (n ϭ 186: dasatinib n ϭ 88, nilotinib n ϭ 98) in sequential phase 2 trials. Entry criteria were similar for all trials. CML-CP was as previously defined. 19 Patients were treated on University of Texas MD Anderson Cancer Center Institutional Review Board-approved protocols. Informed consent was obtained in accordance with the Declaration of Helsinki. Response criteria were as previously described. 2 Conventional cytogenetic analysis was done in bone marrow cells using a G-banding technique. At least 20 metaphases were analyzed, and marrow specimens were examined on direct or short-term (24-hour) cultures. MMR was defined as a BCR-ABL/ABL transcript ratio of less than or equal to 0.1% (international scale). A complete molecular response was defined as undetectable transcripts with an assay with sensitivity of at least 4.5-log. 5 The EUTOS score was defined by (7 ϫ basophils) plus (4 ϫ spleen size) where the spleen size was measured in centimeters below the costal margin and basophils as a percentage at baseline. A EUTOS score of more than 87 indicates high risk, and less than or equal to 87 low risk. 18 EFS was measured from the start of treatment to the date of any of the following events: death from any cause at any time, loss of complete hematologic response, loss of major cytogenetic response, or progression to accelerated or blast phases. TFS was measured from the start of treatment to For personal use only. on June 9, 2017. by guest www.bloodjournal.org From the date progression to accelerated or blast phases at any time, last follow-up, or death from any cause. Survival probabilities were estimated by the Kaplan-Meier method and compared by the log-rank test. Differences among variables were evaluated by the 2 test and Mann-Whitney U test for categorical and continuous variables, respectively. A total of 465 patients were treated. The median (range) age was 47 years (15-85 years). The median (range) time from diagnosis to TKI therapy was 1 month (0-6 months), with 119 (25%) receiving previous cytoreduction therapy. The median (range) follow-up was 117 months (16-130 months) for the patients receiving standarddose imatinib (n ϭ 71), 88 months (4-118 months) for those receiving high-dose imatinib (n ϭ 208), and 30 months (3-69 months) for those receiving second-generation TKI (n ϭ 186). The median (range) basophil percentage at baseline was 3% (0%-19%), and the median (range) splenomegaly size was 0 cm (0-30 cm). A total of 319 patients (69%), 112 (24%), and 34 (7%) were in low, intermediate, and high Sokal score category, respectively. The overall CCyR and MMR rates for the whole study group were 92% and 85%, respectively. The overall CCyR rates were 87%, 91%, and 95%, for patients treated with standard-dose imatinib, high-dose imatinib, and second-generation TKIs, respectively. The overall MMR rates were 78%, 86%, and 86%, respectively. The 3-year EFS, TFS, and OS rates for the whole group were 86%, 95%, and 97%, respectively. The 3-year EFS rates were 80%, 85%, and 91% for patients treated with standarddose imatinib, high-dose imatinib, and second-generation TKIs, respectively. The 3-year TFS rates were 89%, 95%, and 99%, respectively. The 3-year OS rates were 93%, 97%, and 99%, respectively. Overall, of the 465 patients, 427 (92%; 67 [94%] receiving standard-dose imatinib, 189 (91%) high-dose imatinib, 171 [92%] second-generation TKI) were in the low EUTOS score category (Table 1) . Patients with a low EUTOS score had higher rates of CCyR at any time compared with patients with high EUTOS score
The EUTOS score was developed to predict cytogenetic response to IM therapy and failed to predict MMR REF .
EUTOS score is not predictive for survival and outcome in patients with early chronic phase chronic myeloid leukemia treated with tyrosine kinase inhibitors: a single institution experience
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