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license: mit
---

Out of **20,577** human proteins (from [UniProt human proteome](https://www.uniprot.org/proteomes/UP000005640)), sequences shorter than 20 amino acids or longer than 512 amino acids were removed, resulting in a set of **12,703** proteins. The uShuffle algorithm ([python pacakge](https://github.com/guma44/ushuffle)) was then used to shuffle these protein sequences while maintaining their triplet distribution. The sequences for which uShuffle failed to create a shuffled version were eliminated.
Afterwards, h-CD-HIT algorithm ([web server](http://weizhong-lab.ucsd.edu/cdhit-web-server/cgi-bin/index.cgi)) was used with three subsequent filter stages at pairwise identity cutoffs of 0.9, 0.5 and 0.1, resulting in a total of **3,688** sequences.