Table:
0
A
B
C
D
E
F
G
1
Sequencer
Ion Torrent PGM
454 GS FLX
HiSeq 2000
SOLiDv4
PacBio
Sanger 3730xl
2
Manufacturer
Ion Torrent (Life Technologies)
454 Life Sciences (Roche)
Illumina
Applied Biosystems (Life Technologies)
Pacific Biosciences
Applied Biosystems (Life Technologies)
3
Sequencing Chemistry
Ion semiconductor sequencing
Pyrosequencing
Polymerase-based sequence-by-synthesis
Ligation-based sequencing
Phospholinked fluorescent nucleotides
Dideoxy chain termination
4
Amplification approach
Emulsion PCR
Emulsion PCR
Bridge amplification
Emulsion PCR
Single-molecule; no amplification
PCR
5
Data output per run
100-200 Mb
0.7 Gb
600 Gb
120 Gb
100-500 Mb
1.9∼84 Kb
6
Accuracy
99%
99.9%
99.9%
99.94%
90%
99.999%
7
Time per run
2 hours
24 hours
3–10 days
7–14 days
2 hours
20 minutes - 3 hours
8
Read length
200-400 bp
700 bp
100x100 bp paired end
50x50 bp paired end
1,500-5,000 bp (avg)
400-900 bp
9
Cost per run
$350 USD
$7,000 USD
$6,000 USD (30x human genome)
$4,000 USD
$300 USD
$4 USD (single read/reaction)
10
Cost per Mb
$1.00 USD
$10 USD
$0.07 USD
$0.13 USD
$2.00 USD
$2400 USD

Questions and formulas:
Question: What is the sequencer when ion torrent pgm is 200-400 bp?
Formula: UNIQUE(CHOOSECOLS(FILTER(A1:G1,B1="200-400 bp"),1))
Formula2: UNIQUE(CHOOSECOLS(FILTER(A2:G10,B2:B10="200-400 bp"),1))
Level: medium
Question: How many times was Sanger 3730xl $2400 usd?
Formula: ROWS(UNIQUE(FILTER(B1, G1="$2400 USD")))
Formula2: ROWS(UNIQUE(FILTER(B2:B10, G2:G10="$2400 USD")))
Level: medium
Question: How much was solidv4 when pacbio is $300 usd?
Formula: UNIQUE(CHOOSECOLS(FILTER(A1:G1,F1="$300 USD"),5))
Formula2: UNIQUE(CHOOSECOLS(FILTER(A2:G10,F2:F10="$300 USD"),5))
Level: medium
Question: What is solidv4 when sequencer is data output per run?
Formula: UNIQUE(CHOOSECOLS(FILTER(A1:G1,A1="Data output per run"),5))
Formula2: UNIQUE(CHOOSECOLS(FILTER(A2:G10,A2:A10="Data output per run"),5))
Level: medium
Question: WHat is hiseq 2000 when 454 gs flx is 700 bp?
Formula: UNIQUE(CHOOSECOLS(FILTER(A1:G1,C1="700 bp"),4))
Formula2: UNIQUE(CHOOSECOLS(FILTER(A2:G10,C2:C10="700 bp"),4))
Level: medium
Question: What is 454 gs flx when pacbio is 100-500 mb?
Formula: UNIQUE(CHOOSECOLS(FILTER(A1:G1,F1="100-500 Mb"),3))
Formula2: UNIQUE(CHOOSECOLS(FILTER(A2:G10,F2:F10="100-500 Mb"),3))
Level: medium