Original: No team as a # 16 seed has ever defeated a # 1 seed since the field was expanded to 64 teams, though some have come close. As noted above, despite numerous instances of early-round tournament upsets, no # 1 seed has ever lost in the first round to a # 16 seed.
Simplified: Then the 1 seed plays the 16 seed, the 2 seed plays the 15 seed, and so on.