Optimum pH is 7.0.
Optimum temperature is 65 degrees Celsius. Loses half of its activity in 15 min at 90 degrees Celsius.
Optimum pH is 8.0. Displays half maximal activity between pH 6.0 and 9.8.
Optimum temperature is 37 degrees Celsius. It has strong activity at 37 degrees Celsius but is reversibly inactivated in temperatures between 37 and 65 degrees Celsius. Minimal loss of activity is observed in enzyme stored at -10 degrees Celsius in the presence of 15% glycerol.
Optimum pH is 8.0 with glutamate as substrate.
Optimum temperature is higher than 97 degrees Celsius with glutamate as substrate.
Optimum pH is 5.8 for dehydration of anhydrofructose and 6.8 for isomerization of ascopyrone M (PubMed:15716041). Optimum pH is 6.0 for dehydration of 2-dehydro-D-glucopyranose (D-glucosone) (PubMed:8352649).
Optimum pH is 9.3 for the dehydrogenase reaction at 25 degrees Celsius, and 7.0 for the reductase reaction at 25 degrees Celsius.
Optimum pH is 8.0. The pH has no influence on the tropine to pseudotropine product ratio.
Optimum pH is 8.1.
Optimum temperature is 90 degrees Celsius.
Optimum pH is 6.5-7.0.
Optimum pH is 9.7.
Optimum temperature is 75 degrees Celsius.
Optimum pH is between 8 and 9.
Shows a working pH range between 6.0 and 8.0.
Optimum temperature is between 37 and 45 degrees Celsius (PubMed:33713143). Loses most of its activity above 55 degrees Celsius (PubMed:33713143).
Optimum pH is 5.5.
Optimum pH is 9.
Optimum pH is 3-9.
Thermostable.
Optimum temperature is 25 degrees Celsius.
Optimum pH is between 5.5 and 7.
Optimum pH is 6.5 to 7.5.
Optimum pH is 8.8.
Optimum temperature is 45 degrees Celsius.
Optimum pH is about 8.
Optimum pH is 8.0.
Optimum pH is around 6. Highly active between pH range 4.5-6.5.
Optimum pH is 5.0.
Optimum pH is 7.5.
Optimum pH is 6.0-7.0 for degradative reaction (PubMed:22184213, PubMed:17257168). Optimum pH is 7.0 for synthetic reaction (PubMed:22184213). Optimum pH is 9.0-9.5 for synthetic reaction (PubMed:17257168).
Optimum pH is 8.5.
Thermostable. Activity is stable between 20-40 degrees Celsius. Retains about 70% activity after 60 min at 100 degrees Celsius.
Optimum pH is 9.5. At pH 8.0, the activity is reduced by 50%.
Optimum pH is 8.0-9.0.
Optimum temperature is 33-37 degrees Celsius. Active from temperatures ranging from 0 degrees Celsius to 65 degrees Celsius.
Optimum pH active over a broad range of pH values.
Optimum pH is 7.4.
Optimum temperature is 75 degrees Celsius. Thermostable. Retains 20% activity after incubation at 100 degrees Celsius for 1 hour, 80% after 16 hours at 70 degrees Celsius, while no loss of activity is observed after 16 hours at 50 degrees Celsius.
Optimum pH is 7.6.
Optimum pH is 8.4-9.6.
Optimum pH is 7.0 for propionaldehyde reduction and 9.0 for 1-propanol dehydrogenase.
Optimum pH is 5.8.
Optimum temperature is 55-60 degrees Celsius.
Optimum pH is 7.5. The activity is dramatically affected under alkaline conditions (pH 9.5).
Optimum temperature is between 37 to 50 degrees Celsius. An increase in temperature does increase enzyme activity, and complete reversible denaturation of the enzyme occurs at a temperature close to 60 degrees Celsius.
Optimum pH is 8.5-9.0.
Optimum pH is 7-10.
Optimum pH is 6 and 10 for the reduction and oxidation reactions, respectively.
Optimum temperature is 37 degrees Celsius for the reduction reaction. The enzyme appears mostly inactivated at 60 degrees Celsius.
Optimum pH is between 5 and 6.
Optimum temperature is between 37 and 45 degrees Celsius.
Optimum temperature is 48 degrees Celsius.
Optimum pH is 7.0-7.5.
Optimum temperature is 50 degrees Celsius.
Displays a high degree of stability when incubated at temperatures of up to 121 degrees Celsius for 60 minutes.
Highly thermostable.
Optimum pH is 5-5.5.
Optimum temperature is 70 degrees Celsius.
High activity at pH 4.5-6.8.
Optimum temperature is 30 degrees Celsius. Loses 20% of its maximum activity at 37 degrees Celsius and is nearly inactive above 55 degrees Celsius.
Optimum temperature is 55 degrees Celsius.
Optimum pH is 5.5. Stable above pH 5.5.
Optimum temperature is about 90 degrees Celsius. Highly thermostable. Retains 70% of its maximal activity after heating 1 hour at 90 degrees Celsius, but no decrease in activity was observed within the same time at 80 degrees Celsius.
Optimum temperature is 50 degrees Celsius cuts between 37 and 60 degrees Celsius.
Optimum pH is 8.
Optimum temperature is 50 degrees Celsius (PubMed:26763234). Optimum temperature is 35 degrees Celsius (Ref.3).
Optimum pH is 6.0.
Optimum pH is 8-8.5.
Optimum pH is 9. Activities at pH 8.0 and 9.5 were approx 67% and 85%, respectively, of that at pH 9.0.
Active from pH 7.0 to 9.5.
Optimum pH is 8.0-8.5. Active from pH 4.5 to 10. Stable from pH 4 to 11.
Optimum temperature is 55 degrees Celsius. Thermostable for 2 hours up to 70 degrees Celsius.
Optimum pH is 7.5-8.5.
Stable at acidic pHs. Inhibitory activity is unaffected after 1 hour at pH 3.0 and 96 degrees Celsius. Inactivated after 1 hour at pH 10.7 and 96 degrees Celsius.
Optimum temperature is 25-40 degrees Celsius.
Optimum pH is 8.2 for UPRTase activity.
Optimum pH for hydrolysis of PDC is 8.5 and optimum pH for synthesis of PDC is between 6.0 to 7.5.
Optimum pH is 3.5.
Optimum pH is 2.3.
Optimum pH is 7.7 and 7.3 for L-glutamate deamination and 2-oxoglutarate amination, respectively. Half-maximal activity for deamination is observed at pH 6.9 and 7.8 and that for amination is at pH 6.9 and 7.8.
Low thermostability at 41 degrees Celsius due to the dissociation of the hexamer.
Optimum pH is between 8.5 and 9.5.
Optimum temperature is between 15 and 40 degrees Celsius. At temperatures above 50 degrees Celsius, activity is lost rapidly, and at 55 degrees Celsius, the enzyme is totally inactivated.
Optimum pH is 6.4.
Optimum pH is 4-4.5.
Optimum temperature is higher than 95 degrees Celsius. Retains 60% of its activity after 60 min incubation at 80 degrees Celsius.
Optimum pH is 5 for chromate reductase activity.
Optimum temperature is 70 degrees Celsius for chromate reductase activity.
Optimum temperature is above 70 degrees Celsius. Active up to 95 degrees Celsius.
Optimum temperature is 37 degrees Celsius.
Optimum pH is between 7 and 7.5.
Optimum temperature is 90 degrees Celsius. In the absence of magnesium or manganese ions about 50% of the activity is lost within 100 minutes at 78 degrees Celsius, whereas more than 95% of the activity is retained in presence of 4 mM manganese ions. Magnesium ions are a less effective.
Optimum pH is around 6.2.
Extremely thermostable, maintaining full activity after heating for 1.5 hour at 95 degrees Celsius (PubMed:10777501). Exhibits a broad temperature optimum for activity around 80 degrees Celsius (PubMed:24936520).
Optimum temperature is 80 degrees Celsius.
Optimum temperature is 70 degrees Celsius, ATP, ATP-gamma-S or ADP are required for thermostability.
Optimum pH for NAADP(+) production is 5.0 from either substrate, activity is much higher at pH 5.0 than 7.4.
Optimum pH is 6.5 with estrone 3-sulfate, taurocholate, prostaglandin E2 and L-thyroxine T4 as substrates.
Highly stable from pH 0 to pH 12.
Hyperthermostable.
Optimum pH is 7.25-7.5.
Optimum pH is 6.5.
Active at alkaline pHs (up to pH 12 approximately).
Active over a broad temperature range.
Optimum pH is 9.3 for the dehydrogenase reaction, and 6.4 for the reductase reaction.
Optimum pH is 7.6-8.0.
Optimum pH is 5.75 with dihydroquercetin as substrate.
Optimum temperature is 37-40 degrees Celsius.
Optimum pH is 7.6-8.2.
Optimum temperature is 70-80 degrees Celsius.
Optimum pH is 9.0.
Optimum temperature is 50 degrees Celsius. Active from 10 to 80 degrees Celsius.
Optimum pH is 7.5 at 32 degrees Celsius.
Optimum pH is 3.8.
Optimum temperature is 70 degrees Celsius. Thermostable.
Optimum pH is about 5.5.
Loses 50% of its activity after heating at 80 degrees Celsius for 10 min.
Optimum pH is 9. Highly stable from pH 7.5 to 9.0. At lower pH values, the residual activity decreases rapidly to 50% at pH 6.5, and it is completely abolished after 1 hour of incubation at pH levels of 6.0 and below.
Optimum temperature is 55 degrees Celsius. The activity increases 3-fold in a linear fashion from 25 to 50 degrees Celsius before decreasing slowly at higher temperatures.
Thermolabile.
Optimum pH is 7.5-8.0 for DNA restriction.
Optimum pH is 7.5. The enzyme is stable when incubated for 15 min at 30 degrees Celsius at pH 7.5-9.
Optimum temperature is 45 degrees Celsius. Loss of activity is 0%, 16%, 30%, 82% and 100% when incubated at 25, 30, 40, 50 and 60 degrees Celsius for 30 min, respectively.
Optimum pH is 8.0-8.5. The activity is more than 90% in the pH range from 7 to 9.
Optimum temperature is between 20 and 30 degrees Celsius.
Optimum pH is 8.9. Active from pH 5 to 10.
Thermostable up to 60 degrees Celsius.
Optimum pH is 6.5 for recombinant LGOX expressed in E.coli or in P.pastoris.
Optimum temperature is 30 degrees Celsius for recombinant LGOX expressed in E.coli (PubMed:24657922). Optimum temperature is 40 degrees Celsius for recombinant LGOX expressed in P.pastoris (PubMed:27693490).
Optimum pH is 8.8-9.0.
Optimum pH is 7.2.
Optimum temperature is degrees Celsius.
Optimum pH is 6.9-7.3.
Optimum pH is 8 for amination, and 9 for deamination.
Optimum pH is 6.0. More than 80% of its maximal activity is maintained at pH 5.5-6.5.
Optimum temperature is 50 degrees Celsius. Retains 20% of the maximal activity after incubation at 60 degrees Celsius for 30 min. Retains approximately 15% of the maximal activity at the temperature below 15 degrees Celsius.
Optimum pH is 12.3.
Optimum temperature is 55 degrees Celsius. CaC1(2) markedly shifts the optimum temperature to 70 degrees Celsius.
Stable at pH 3 and 25 degrees Celsius.
Melting temperature is 57.7 and 80.3 degrees Celsius at pH 7 and pH 3, respectively. Not heat stable, unfolds at 95 degrees Celsius at pH 3. Refolds partially at physiological pH 7, but not at pH 3, after being heated to 95 degrees Celsius and cooled down back to 25 degrees Celsius.
Optimum pH is 6.0-8.0.
Optimum pH is 7-7.5.
Optimum pH is above 9.
Optimum pH is about 2.
Optimum pH is 7.0-8.0.
Optimum temperature is 85 degrees Celsius.
Optimum pH is 7.4 for the THF-independent L-allothreonine aldolase activity. More than 95% of full activity remains at pH 8.4, although the activities are reduced to about 90% at pH 6.9 and about 75% at pH 5.9.
Highly thermostable. Retains almost complete THF-independent L-allo-threonine aldolase activity after being incubated at 75 degrees Celsius for 10 minutes, and retains about 77% residual activity after being treated at 87 degrees Celsius for the same time.
Optimum temperature is 25 degrees Celsius (irrespective of the state of osmotic stimulation).
Optimum pH is 6.8 to 7.8.
Optimum pH is 6.5 for ATPase activity.
Optimum temperature is >80 degrees Celsius.
Optimum temperature is 40 degrees Celsius.
Optimum temperature is over 100 degrees Celsius.
Optimum pH is 8.5-8.8.
Optimum temperature is 65 degrees Celsius.
Optimum pH is 8.7 (for protoporphyrinogen oxidation).
Thermostable. Still fully active after heating to 70 degrees Celsius for 10 minutes.
Optimum pH is 7-9.
Retains 38% activity when incubated at 100 degrees Celsius for 10 minutes.
Optimum pH is 6.2-8.0.
Optimum temperature is 60-70 degrees Celsius.
Optimum pH is 8.2. Activity decreases sharply as the pH is raised above pH 9.2.
Active from pH 3.0 and pH 9.0.
Thermostable, retains activity after heating at 50 degrees Celsius for 24 hours. Activity decreases over 70 degrees Celsius.
Optimum pH is 6.0 (with reduced chitobiose as substrate).
Optimum temperature is 80 degrees Celsius (with reduced chitobiose as substrate).
Optimum temperature is 30 degrees Celsius.
Optimum pH is 7.0-7.5 with ATP as substrate, and 7.5-8.0 with ADP as substrate.
The heterodimer of A and B subunits is structurally stable between pH 5-8 in the presence of Ca(2+) and Zn(2+) ions. Stability decreases significantly in the presence of imidazole and glycine. Addition at nanomolar concentrations of glycosaminoglycans (GAGs), such as chondroitin sulfate, dermatan sulfate or hyaluronic acid to the buffer, increases its stability.
Optimum pH is 6-6.5.
Optimum pH is 11 for substrate oxidation and 6.0-7.2 for sugar reduction.
Optimum pH is 9.5 for RpsF modification and 9 for polyglutamate synthase activity.
Shows thermal stability. Exhibits 86% activity after incubation at 55 degrees Celsius for 15 minutes, but its activity decreases sharply at 60 degrees Celsius.
Optimum pH is 6.0-6.6.
Optimum temperature is 35-40 degrees Celsius. Inactive above 60 degrees Celsius. Thermostable up to 55 degrees Celsius.
Optimum pH is 7.5-8.0.
Highly active from pH 3 to 5 with 4-nitrophenyl phosphate as substrate, and from 2.5 to 7.5 with phytic acid.
Able to withstand temperatures up to 100 degrees Celsius over a period of 20 min, with a loss of only 10% of the initial enzymatic activity.
Optimum pH is 7.0. Active from pH 7.0 to 8.5.
Thermostable. Has a 44-minute half-life at 100 degrees Celsius.
Optimum temperature is about 35 degrees Celsius.
Optimum pH is 5.5-6.0 for dihydroxyacetone reduction and 9.5-10.0 for glycerol oxidation (PubMed:40950). Optimum pH is 10.0-10.2 for 1-amino-2-propanol oxidation (for homooctameric form) (PubMed:6365902).
The activity increases with temperature.
Optimum pH is 7.8-8.1.
Optimum pH is 7.5-8.
Highly thermostable. Does not lose activity after 16 hours at 70 or 80 degrees Celsius, but incubation of the enzyme at 90 and 99 degrees Celsius for 1 hour results in gradual loss of activity.
Optimum pH is between 8 and 10.
Moderately thermotolerant.
Optimum pH is 7.1.
Optimum pH is 4.5.
Optimum pH is 7-9 for the lysophosphatidic acid acyltransferase (LPAAT) reaction.
Still active at 100 degrees Celsius. Thermostable.
Optimum pH is 6-8.
Optimum temperature is 40-50 degrees Celsius.
Optimum pH is 5. Activity decreases sharply toward pH 7.
Optimum pH is 6.5-8.0.
Optimum pH is 9.0. Active from pH 6 to 9.
Optimum pH is 8.4-8.8.
Optimum temperature is 46-48 degrees Celsius.
Optimum pH is 6.0 for nitrite oxide generation. Activity decreases below pH 5.0 and above pH 8.0 (PubMed:19801639).
Optimum pH is 7-8 with pelargonidin and cyanidin as substrates.
Optimum temperature is 30 degrees Celsius with pelargonidin and cyanidin as substrates.
Optimum pH is between 8.5 and 9.0.
Optimum temperature is 42 degrees Celsius.
Optimum pH is about 9. Active from pH 7 to 10.
Optimum temperature is 70 degrees Celsius. Inactive at 37 degrees Celsius.
Optimum pH is 7-9 for both activities.
Optimum temperature for both activities is 40 degrees Celsius.
Optimum pH is 7.
Optimum pH is 7-8.5 at 37 degrees Celsius.
Optimum pH is 7.0. The enzyme is slowly inactivated above pH 9 and below pH 5.
Optimum temperature is 50 degrees Celsius. The enzyme is inactivated at temperatures above 40 degrees Celsius.
Stable up to 30 degrees Celsius.
Stable for 7 days at room temperature and for 30 days at 4 degrees Celsius. Remains active after incubation at 100 degrees Celsius for 3 hours.
Activity decreases rapidly below pH 7.5 in phosphate buffer, and below pH 8.0 in Tris buffer.
Highly thermostable. Is active at high temperatures (60 and 80 degrees Celsius).
Optimum pH is 8.5 with DFP as substrate.
Optimum pH is about 6.
Optimum pH is 6-7.
Stable at temperatures below 40 degrees Celsius.
Optimum pH is 8.2 (at 30 degrees Celsius).
Optimum pH is 5.5. Retains more than 50% of activity between pH 4 and 7.
Optimum pH is about 5.0. Stable between pH 4-8.
Enzyme activity is retained almost fully under 40 degrees Celsius and completely destroyed at 70 degrees Celsius. At 60 degrees Celsius the activity was over 80% compared to the untreated enzyme.
Optimum pH is 8.0-8.5 with all-trans-beta-carotene as substrate.
Optimum pH is 6.0 with dGMP or dCMP as substrate.
Optimum pH is 5.0. At least 20% of maximum activity is retained between pH 4.0 and 7.0.
Optimum temperature is 50 degrees Celsius. After 30 min incubation at 50 degrees Celsius in the absence of substrate 50% of activity is lost.
Optimum pH is 7 with 4-nitrophenyl phosphate as substrate.
Optimum temperature is 65 degrees Celsius with 4-nitrophenyl phosphate as substrate.
Optimum pH is 8.0. Totally inactive at pH 9.0.
Optimum pH is 2.5 or below with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) or 2,6-dimethoxy-phenol as substrate, and 3.5 with guaiacol as substrate.
Optimum pH is 8 for OATase activity and 8-8.5 for AGSase activity.
Optimum temperature is 75 degrees Celsius (OATase activity).
Stable after heating at 69 degrees Celsius for 5 hours.
Optimum pH is 8.5 for the reductase activities and 9.0 for the dehydrogenase activities.
Optimum pH is 8-9.
Optimum pH is 7.5-8.0 (for taurine transport).
Optimum temperature is about 39 degrees Celsius.
Optimum pH is 8.5-9.5.
Optimum pH is 6.0 for histamine uptake (PubMed:22396231). Optimum pH is 6.6 for adenosine, serotonin and inosine uptake (PubMed:20592246). Optimum pH is 6.0 for adenosine uptake (PubMed:16873718, PubMed:31537831). Does not transport adenosine at pH 7.4 (PubMed:16873718).
Optimum pH is below 2.5.
Optimum pH is 7.75.
Optimum pH is between 6 and 7.5.
Optimum pH is 7.5-10.5. Active from pH 5.5-11.5.
Optimum pH is 9.5.
Optimum pH is 8.0-8.5.
Optimum pH is 7.5 in the forward direction and 6.0 in the reverse direction.
Optimum pH is 8.5. Active from pH 7-10.
Optimum temperature is 65-70 degrees Celsius.
Optimum pH is 8.8. At the physiological pH of 7, activity is only about 30%.
Optimum pH is 7.2 with only 50% of this activity retains at pH 6.2 or 8.0.
Optimum temperature is 20 degrees Celsius.
Optimum pH is 6.8 for reduction (forward reaction) and 9.0 for oxidation (reverse reaction).
Optimum temperature is 28 degrees Celsius for reduction (forward reaction) and 30 degrees Celsius for reduction (reverse reaction).
Optimum pH is 6.5. No activity is detected at pH below 4.5.
Optimum temperature is 70 degrees Celsius. It retains more than 75% of activity after incubation at 85 degrees Celsius and the half-life at 90 degrees Celsius is 1 hour. Thermostable.
Optimum pH is 7.0 in phosphate buffer.
Optimum pH is 7-8.5.
Optimum pH is 7.0-9.0.
Optimum pH is around 8.0.
Optimum temperature is 35 degrees Celsius.
Optimum pH is 7.8. Active from pH 4.0 to pH 9.0.
Optimum temperature is 45 degrees Celsius. Active from 30 to 55 degrees Celsius.
Hemagglutinating activity stable between pH 6 and 8.
Hemagglutinating activity stable up to 60 degrees Celsius but diminishes with higher temperatures and is absent at 100 degrees Celsius.
Optimum pH is 7.5-8.5 with t-butoxycarbonyl-GRR-aminomethylcoumarin as substrate.
Optimum pH is 5.5-8.5.
Thermo-tolerant.
Optimum temperature is 15-37 degrees Celsius. Active over a broad range of temperatures, from 4 to 80 degrees Celsius.
Optimum pH is 7.0-8.5.
Optimum pH is 6.5 with estrone 3-sulfate and L-thyroxine (T4) as substrates.
Optimum temperature is 60 degrees Celsius. Stable at 55 degrees Celsius. Retains 80% of activity at 70 degrees Celsius.
Optimum pH is about 7.5. The activity at pH 6.5 and 8.6 is only about 20% of the maximal achievable activity at optimum pH.
Optimum pH is 6.0-9.0.
Optimum temperature is 37-41 degrees Celsius.
Optimum pH is 7.0 for the NAD(+)-dependent oxidation of vanillin.
Optimum temperature is 30 degrees Celsius for the NAD(+)-dependent oxidation of vanillin. No significant loss of activity at 4 degrees Celsius after two weeks.
Optimum pH is 6 at 25 degrees Celsius (PubMed:34093489). Retains over 60 percent activity in the pH range from 4 to 10 (PubMed:34093489). Retains activity above 50% after incubation at pH 5-10 for 72 hours (PubMed:34093489).
Optimum temperature is 50 degrees Celsius (PubMed:34093489). Retains over 57 percent activity when incubated for 2 hours at temperatures between 30-65 degrees Celsius (PubMed:34093489).
Optimum pH is 3.0-4.5.
Optimum pH is 5.3. Active from pH 4.2 to 6.5.
Optimum pH is 7. About 20% of maximum TPADO activity is observed at pH 9, whereas no activity is observed at pH 5.
Optimum temperature is 30 degrees Celsius. Approximately 60% of maximum TPADO activity is observed at 15 degrees Celsius, and activity is completely lost at 50 degrees Celsius.
Optimum temperature is 50-55 degrees Celsius.
Optimum pH is 6.0-6.50.
Highly thermostable. No decrease in activity was observed after heating 15 minutes at 80 degrees Celsius.
Optimum pH is 5.
Optimum pH is 5.5 for the peroxidase reaction and 6.0 for the catalase reaction. Active from pH 5.5 to 8.5.
Optimum temperature is 40-45 degrees Celsius.
Optimum pH is 8. At pH below 7.0 or above 9.0, there is substantial loss of activity.
Optimum temperature is 25 degrees Celsius. The enzyme activity measured at 5 degrees Celsius is maintained up to 25 degrees Celsius but is lost quickly at higher temperatures.
Optimum pH is between 8 and 8.4 (PubMed:12379101). In the thioesterase domain, the size of the substrate channel increases with increasing pH (PubMed:12379102).
Optimum pH is 6.8-7.8.
Optimum pH is 5 for the reduction of methylglyoxal.
Optimum temperature is 60 degrees Celsius for the reduction of methylglyoxal.
Optimum pH is between 6.75-7.25.
Optimum pH is 4.5 with N-palmitoylethanolamine as substrate.
Optimum pH is 7.25-7.75.
Optimum pH is 8.5 for glucose-1-phosphate thymidylyltransferase activity.
Optimum temperature is 95 degrees Celsius for glucose-1-phosphate thymidylyltransferase activity.
Optimum pH is 9.4 for AMP incorporation, 10.0 for CMP incorporation, and 7.0 for the phosphatase and phosphodiesterase activities.
Optimum pH is 4.5. Stable from pH 4.0-7.5, activity decreases drastically above pH 7.5 and below pH 3.5.
Optimum temperature is 70 degrees Celsius. Active from 30 to 75 degrees Celsius, inactivated following 150 minutes incubation at 85 degrees Celsius.
Optimum extracellular pH is 4.5 for sorbitol as substrate.
Optimum pH is 9.0 in the forward reaction.
Optimum pH is 7.5. No activity at pH above 9.5 or below 6.5.
Optimum pH is 6.5 with estrone 3-sulfate, taurocholate, prostaglandin E2 and L-thyroxine (T4) as substrates.
Optimum pH is 7.0-7.5 for the reduction of lipoate by NADH.
Optimum temperature is 50 degrees Celsius for the reduction of lipoate by NADH.
Optimum pH is from 5.5 to 9.0 for xylosyltransferase activity. Optimum pH is 5.5 for Beta-1,3-glucuronyltransferase activity.
Optimum pH is 9-10 for hemagglutinating activity. The activity is decreased by 25% at pH 6.0 and by 50% at pH 4.0.
Thermally labile. Complete loss of hemagglutinating activity after incubation at 60 degrees Celsius for 30 min.
Stable from pH 2 to 12.
Displays a high degree of stability when incubated at temperatures between -80 and 75 degrees Celsius for 60 minutes. Autoclaving the peptide at 121 degrees Celsius for 15 minutes had no effect but incubation at 100 degrees Celsius for 60 minutes caused a 32-fold reduction in activity.
Optimum pH is 8.0 with tributylglycerol as substrate, and 8.2 with retinyl palmitate as substrate. Active from pH 4.5-9.5 with retinyl palmitate as substrate.
Optimum temperature is 55 degrees Celsius at pH 8.0.
Optimum pH is 7-8.
Optimum pH is 6.
Optimum pH is 6.8.
Optimum pH is 8 for hydrogenase activity at >95 degrees Celsius.
Optimum temperature is greater than 95 degrees Celsius for hydrogenase activity. Stable up to 100 degrees Celsius. Loses 50% of activity at 80 degrees Celsius after 21 hour incubation.
Optimum pH is 9-11.
Optimum pH is 70.
30-min incubation at 70 degrees Celsius drastically reduces the enzyme activity to 11% of the initial value, and 77% of the activity remains after 30 min of incubation at 42 degrees Celsius.
Optimum pH is 8.0-8.8.
Optimum temperature is 49 degrees Celsius.
Optimum pH is 6.5-8.
Optimum pH is 6.4. Activity decreases sharply at pH below 5.0.
Optimum temperature is 37 degrees Celsius. Less active at room temperature and shows very little activity at 4 degrees Celsius. Loses activity at 57 degrees Celsius within 5 minutes.
Optimum temperature is 88 degrees Celsius.
Optimum pH is 9.0 for ThTP synthesis.
Optimum pH is 9.0 for the forward reaction and 8.0 for the reverse reaction.
Optimum temperature is 30-50 degrees Celsius.
Optimum temperature is 50-70 degrees Celsius.
Optimum pH is 7.0 (with pNP-butyrate as substrate).
Optimum temperature is 40 degrees Celsius (with pNP-butyrate as substrate).
Optimum pH is 7.5. Active between pH 6 and 9.
Optimum pH is 4.5. the enzyme has a second pH optimum at pH 9.
Optimum pH is 6.7-6.9.
Optimum pH is 7.5-8.0 for the forward reaction and 6.0-6.5 for the reverse reaction.
Optimum pH is 8.2. At 40 degrees Celsius (temperature of natural environment) the optimum pH is 7.9.
Optimum pH is 4.0. Stable between pH 6.0 and 9.0.
Optimum temperature is 50 degrees Celsius. Stable below 50 degrees Celsius.
Optimum pH is 9.0. At pH 9.5, retains more than 60% of its maximum activity. At pH 7.0 the relative activity is less than 10%.
Optimum pH is 4.4-4.8 at 35 degrees Celsius. PG1 is resistant to acidic but not to alkaline conditions, at which PG2 is released from the beta subunit.
Optimum temperature is 55-60 degrees Celsius at pH 4.4. PG1 is more thermostable than PG2.
Optimum pH is 6-9.
Optimum temperature is 90 degrees Celsius. Active from 70 to 90 degrees Celsius.
Optimum pH is 6.3.
Optimum temperature is 22.5 degrees Celsius.
Optimum pH is 7.5-9.5.
Optimum pH is around 6.5.
The level of spermidine acetyltransferase activity increases at low temperature to prevent spermidine toxicity.
Optimum pH is 8.4.
Optimum temperature is around 40 degrees Celsius. Activity decreases significantly at temperatures above 45 degrees Celsius.
Optimum pH is 8. The enzyme is stable between 7 and 10.
Optimum temperature is around 60 degrees Celsius. The enzyme is stable below 60 degrees Celsius.
Optimum pH is 8.5-9.0 for sodium transport and 8.5 for lithium transport. Na(+)/H(+) antiporter activity is detected between pH 6.5 and 9.5, whereas Li(+)/H(+) antiporter activity is detected only above pH 7.5.
Optimum pH is 6.5-9.
Optimum pH is near neutral pH.
Optimum pH is 8.2.
Optimum pH is 10.0.
Optimum temperature is about 30 degrees Celsius.
Optimum pH is between 6.5 and 6.9.
Optimum pH is between 4.5-6.5. Loses activity rapidly when pH is lowered from 5 to 4.
Optimum temperature is 30X degrees Celsius.
Stable from pH 3.0-9.0, inactivated at pH 10.0.
Thermostable, activity is retained after incubation at 100 degrees Celsius for 15 minutes.
Optimum pH is 8.5. Is stable in the pH 6-9.5 range. A great decrease in stability is only produced at pH values below pH 4.0.
Thermostable. Displays a Tm value of 2.5 hours at 60 degrees Celsius, and 0.6 hour at 80 degrees Celsius.
Optimum pH is 7.2 for cholesterol ester hydrolysis.
Optimum pH is 6.5-7 at 23 degrees Celsius.
Optimum pH is 7.5 to 9.0. Retains half maximal activity at about pH 6.5 and 10.5 and is inactive at pH 5 and below.
Optimum pH is 7. Stable from pH 4 to pH 7.
Optimum pH is 7.0 to 8.0 for 4Fe-4S stability.
Optimum pH is 7-8 for hemagglutinating activity. Activity drops rapidly at lower or higher pH.
Thermostable. Retains hemagglutinating activity after incubation at 50 degrees Celsius for 1 hour. At higher temperatures activity drops drastically and is lost at 80 degrees Celsius.
Optimum pH is 7.0 for MAG lipase activity. Active over a broad pH range from pH 6 to pH 9 (PubMed:26991558). Optimum pH is 8.5 for p-nitrophenyl palmitate and pH 7.5 for both p-nitrophenyl acetate and p-nitrophenyl butyrate (PubMed:29225428).
Optimum temperature is 30 degrees Celsius for MAG lipase activity (PubMed:26991558). Optimum temperature is 45 degrees Celsius for p-nitrophenyl palmitate and 30 degrees Celsius for both p-nitrophenyl acetate and p-nitrophenyl butyrate (PubMed:29225428).
Optimum pH is 7-9.5. Is most active at slightly alkaline pH values and exhibits a sharp decrease in its catalytic activity at low pH. The drop in LpxH activity at acidic pH is not due to enzyme instability as preincubation of the enzyme at low pH does not alter the apparent enzyme activity at the standard condition of pH 8.0.
Optimum pH is about 8.0.
Optimum pH is 4.0-6.8.
Optimum pH is 6.0-7.0.
Optimum pH is 6.5 to 7.0. Above pH 7.1 molybdenum is released.
Optimum temperature is 37 degrees Celsius for acetylserotonin O-methyltransferase activity.
Optimum pH is 6.0-6.5.
Optimum temperature is 30-38 degrees Celsius.
Optimum pH is 6.6.
Optimum pH is 6.0 for the transport of the drug quinidine. No transport activity is observed at pH 7.5, possibly due to the protonation of quininide at pH6.0 (PubMed:11408531). TEA transport is independent of any pH (PubMed:8955087).
Optimum pH is 6.8 with NADPH as substrate and 6.8-7.8 with NADH as substrate.
Optimum pH is 5.5 for the free enzyme, and pH 6 in the presence of bound integrins.
Optimum pH is 9 for the forward reaction which is the transfer reaction of the amino group from GABA to beta-ketoglutarate, and pH 8 for the reverse reaction.
Optimum temperature is 80-90 degrees Celsius.
Optimum pH is 4.7 with ABTS as substrate. Optimum pH is 7.2 with benzyl alcohol as substrate, and there is another optimum at pH 6.2. Optimum pH is 7.2 with dimethoxyphenol as substrate. Optimum pH is 6.0 with naphthalene as substrate. Optimum pH is 7.0 with pyridine as substrate. Optimum pH is 7.0 with veratryl alcohol as substrate, and there is another optimum at pH 5.5.
Exhibits unusual thermal stability. At pH 7.4, the enzyme retains full activity after incubation at 25 degrees Celsius for 30 days. Is stable at alkaline condition and is not inactivated by freezing.
Optimum temperature is 30 degrees Celsius. Stable up to 60 degrees Celsius.
Optimum temperature is 30-37 degrees Celsius.
Optimum pH is 6.2.
Optimum temperature is 67 degrees Celsius. 50 perscent maximal activity is exhibited at 75 degrees Celsius. Reaction time was 10 min.
Retains 97% of original activity when incubated for 200 hours at 60 degrees Celsius.
Optimum pH is around 7.0. Retains about 90% of maximum activity at pH 8.0.
Optimum temperature is 120 degrees Celsius. Activities at 100 and 80 degrees Celsius are about 50% and 10% of that at 120 degrees Celsius, respectively. Is unstable so that the protein loses about 45% of its activity after heating for 15 minutes at 100 degrees Celsius. However, the enzyme is not so intensively inactivated in the presence of ATP.
Optimum pH is 6.5. Active from 5.5 to 9.0 with 4-oxo-L-proline.
Optimum pH is 7.8.
Heat-stable to about 95 degrees Celsius.
Optimum pH is 5.0-10.5.
Optimum temperature is 45-50 degrees Celsius.
Optimum pH i 9.3.
Optimum pH is 6.3-6.8.
Optimum pH is 8.4-8.9.
Optimum pH is 9-9.5.
Optimum pH is 6.25.
Optimum pH is 5.0-5.5 and 6.5 for the phosphatase activity with 3'-UMP and 5'-UMP as substrate, respectively.
Optimum temperature is 45 degrees Celsius. The activity is undetectable below 20 and above 55 degrees Celsius.
Optimum pH is 5.0-5.5 when using synthetic substrate p-nitrophenyl-alpha-D-galactopyranoside.
Optimum temperature is 90-95 degrees Celsius when using synthetic substrate p-nitrophenyl-alpha-D-galactopyranoside. The half-life of thermoinactivation is 6.5 h at 85 degrees Celsius.
Optimum pH is 10.5.
Optimum temperature is 26 degrees Celsius.
Optimum pH is 7.5 in Tris/HCl buffer.
Optimum temperature is 30-35 degrees Celsius. Has a half-life of about 80 minutes at 25 degrees Celsius.
Optimum pH is 8.4. 85% of activity remains at pH 9.0, 54% of activity remains at pH 7.0.
Optimum pH is 4.5 to 9.5 using 24-mer 5'-OH DNA as substrate and 4.5 to 6.5 using 24-mer 5'-OH RNA as substrate.
Optimum temperature is 55 to 75 degrees Celsius using 24-mer 5'-OH DNA as substrate and from 55 to 85 degrees Celsius using 24-mer 5'-OH RNA as substrate.
Optimum pH is 3.5 (PubMed:24848382, PubMed:28156112). More than 90% of the maximal activity is maintained between pH range 3-5 (PubMed:24848382). More than 60% of the maximal activity is maintained between pH range 2-8 (PubMed:24848382, PubMed:28156112). About 80% of the maximal activity is maintained even at pH 2.5 (PubMed:28156112).
Optimum temperature is around 40-50 degrees Celsius (PubMed:24848382, PubMed:28156112). Has more than 50% of the maximal activity between 10-70 degrees Celsius, and more than 30% of activity at 0 degrees Celsius. Stable at 50 degrees Celsius for 30 min (PubMed:24848382). 60-80% of the maximal activity is retained between 0-10 degrees Celsius. Displays 40% of its maximal activity at as high as 80 degrees Celsius. Remains active at 60 degrees Celsius for over 8 hours. Approximately 50% of the activity is still maintained after 4-hour incubation at 70 degrees Celsius (PubMed:28156112).
Optimum pH is 7.4-7.6.
Optimum pH is 5.3.
Optimum pH is 5-7. At higher pH levels, ferric reductase activity rapidly declines while cupric reductase activity remains high.
Optimum temperature for dsDNA cleavage is 46 degrees Celsius and occurs between 37-52 degrees Celsius, no cleavage at 55 degrees Celsius (PubMed:32246713). Optimum temperature for ssDNA cleavage is 45-55 degrees Celsius (PubMed:30337455).
Optimum pH is between 7.5 and 9.5.
Optimum pH is 8.6.
Optimum temperature is 40 degrees Celsius. It loses most of its activity at 55 degrees Celsius.
Optimum pH is 9-10.
Optimum temperature is 60 degrees Celsius.
Stable at 80 degrees Celsius for 15 minutes.
Optimally active at acidic pHs.
Optimum pH is 6.0. Active from pH 4 to 8.
Optimum pH is 6-8.5.
Active up to pH 11.
Active up to 100 degrees Celsius.
Optimum pH is 6.5. Is extremely stable over a wide pH range: upon heating at 50 degrees Celsius for 60 minutes, the enzyme does not lose activity at pH 4.5-11.0.
Extremely thermostable. Retains full activity upon heating at 100 degrees Celsius for 10 minutes and at 80 degrees Celsius for 60 minutes.
Optimum pH is 7.9.
Optimum pH is 8.5 in glycine-NaOH. Optimum pH is between 7.5 and 9.5 in potassium phosphate, Tris-HCl and Tris-acetate.
Optimum temperature is 43 degrees Celsius. Quite stable as loses only 5% of activity in 1 h at 30 degrees Celsius, 12% at 40 degrees Celsius and 25% at 50 degrees Celsius.
Optimum pH is 5.0-7.5.
Resistant to thermal inactivation at 50 degrees Celsius.
Optimum pH is 5-8.
Optimum temperature is 60 degrees Celsius. Retains more than 90% activity after 24 hours at 50 degrees Celsius.
Optimum temperature is 95 degrees Celsius.
Optimum hemagglutination activity at pH 7.4. Retains its hemagglutination activity throughout pH range 3-10. This protein is most stable at pH 7.4.
Optimum hemagglutination activity between 37 and 40 degrees Celsius. Retains its hemagglutination activity up to 60 degrees Celsius, but loses the activity at higher temperatures. This protein is stable at high temperatures over 80 degrees Celsius. The thermal stability is increased by the presence of ligand D-galactose.
Optimum temperature is 30 degrees Celsius. Activity is lost above 40 degrees Celsius.
Optimum pH is 5.5 with larch arabinogalactan as substrate.
Optimum temperature is 50 degrees Celsius with larch arabinogalactan as substrate.
Optimum pH is 4.0.
Most stable at pH 8.0. Unstable at acidic pH values.
Optimum pH is about 6.0.
Optimum pH is 6.7-6.8.
Optimum pH is 8.5 at room temperature (with LTA4 as substrate) (PubMed:9744798). Optimum pH is 7.5 at room temperature (with alanine-4-nitroanilide as substrate) (PubMed:9744798).
Optimum temperature is 30 degrees Celsius with tosyl-Arg-methyl ester (TAME) as substrate and 37 degrees Celsius with natural substrates.
Optimum pH is 8.5 for uptake.
Optimum pH is 6.8-7.
Optimum pH is 6.75-7.25.
Optimum temperature is 40 degrees Celsius (at pH 8). Active from 10 to 50 degrees Celsius.
Optimum pH is 8.5 with CGGAIISDFIPPPR peptide as substrate.
Optimum pH is 7.5-9.0.
Optimum pH is 8.5-9.
Optimum pH is 6.8 for reduction (forward reaction) of codeinone to codeine and 9.0-10.0 for the oxidation (reverse reaction) of codeine to codeinone.
Optimum temperature is 30-42 degrees Celsius.
Optimum pH is 7.0 for PNP, and 5.5 for PPi.
Optimum pH is 11 for malate oxidation.
Optimum pH is 8.0 for isoforms 1, 2 and 3.
Isoforms 1 and 3 have maximum activity from 45 to 55 degrees Celsius.
Optimum pH is about 10.5. No activity is lost after 30 minutes incubation at pHs between 5.0 and 11.0.
Optimum temperature is 65 degrees Celsius. Thermostable. No activity is lost after 30 minutes incubation at temperatures below 60 degrees Celsius, while half of the activity is lost after 30 minutes incubation at 65 degrees Celsius.
Optimum temperature is 42 degrees Celsius. ATPase activity is 1.5-fold decreased at 25 degrees Celsius and 37 degrees Celsius, 4.7-fold at 20 degrees Celsius. The protein is not active at 50 degrees Celsius.
Thermolabile at 90 degrees Celsius.
Optimum temperature is 55 degrees Celsius. The enzyme activity decreases rapidly at temperatures above 60 degrees Celsius.
Optimum pH is 9.0-9.5.
Optimum pH is between 7.0 and 7.5.
Optimum pH is 8.1 at room temperature. Retains half maximum activity at pH 6.5 and pH 9.5 at room temperature.
Thermolabile. Inactive at 46 degrees Celsius.
Stable at high temperatures.
Optimum pH is 7.7.
Optimum temperature is 95-100 degrees Celsius.
Optimum pH is 5.7-6.
Optimum temperature is 70-85 degrees Celsius.
Optimum pH is 4.5-6.0.
Optimum temperature is 50-60 degrees Celsius.
Has a half-life of 15 minutes at 30 degrees Celsius.
Optimum pH is from 5.5 to 8.5.
Activity increases with temperature elevation from 65 degrees Celsius to 95 degrees Celsius.
Optimum temperature is 50 degrees Celsius. Thermostable. Active from 20 to 80 degrees Celsius.
Optimum pH is 8.0. Stable from pH 6.0 to 9.0.
Optimum temperature is 45 degrees Celsius, activity decreases rapidly above 45 degrees Celsius possibly due to instability at higher temperatures. Inactivated following 10 minutes incubation at 55 degrees Celsius, and only retains 2.6% of activity after 10 minutes incubation at 50 degrees Celsius.
Optimum pH is 7.8-8.5.
Most active from pH 5.5 to 8.0. Inactive below pH 4.3.
Optimum temperature is between 50 and 60 degrees Celsius.
Optimum pH is 7.5-8. Active from pH 6 to 9.
Optimum pHs for antimicrobial activity are 3.0 and 6.0. Activity is reduced by 50% at alkaline pHs 9.0 and 11.0.
No change in antimicrobial activity between 80 or 100 degrees Celsius after 15 minutes, but reduction in 50% antimicrobial activity at 121 degrees Celsius after 15 minutes.
Optimum pH is 8.5 for agmatine and MPP(+) transport.
Optimum temperature is 37.3 degrees Celsius.
Stable between pH 3.0 and pH 7.0.
Thermostable, retains activity after heating at 100 and 121 degrees Celsius.
Thermostable. Upon heating, unfolds only partially even at 95 degrees Celsius. Upon cooling to room temperature, almost completely restores its structure, indicating high heat stability and refolding capacity.
Optimum temperature is above 95 degrees Celsius.
Optimum temperature is 70 degrees Celsius for the cystathionine beta-lyase activity.
Optimum pH is acidic.
Optimum temperature is 23-38 degrees Celsius.
Optimum temperature is above 90 degrees Celsius.
Optimum pH is between 5.6 and 6.
Optimum pH is 6.0, activity is seen from pH 5.5 to 8.0.
Optimum pH is about 7.2.
Optimum pH is 9. Active from pH 8 to 10.
Optimum pH is 6.8-7.2.
Optimum pH is 5.0-5.5.
Optimum pH is 8.0 with 3-oxo-C8-HSL as substrate. Activity increases with increase in pH from 6.0 to 8.0, and declines slightly at pH 9.0. Little or no activity at pH 5.0 or below.
Stable below 37 degrees Celsius, activity decreases sharply after incubation for 2 hours at 45 degrees Celsius.
Activity increases with increasing temperatures between 4 and 42 degrees Celsius. Activity remains near 80% of the maximum after exposure at 37 degrees Celsius for 30 minutes.
Optimum pH is 7.3 with D-mannose as substrate.
Optimum temperature is 30 degrees Celsius with D-mannose as substrate.
Optimum temperature is 39 degrees Celsius.
Denaturation temperature (Td) is 101.2 degrees Celsius.
Thermostable for 60 minutes up to 50 degrees Celsius.
Thermostable up to 55 degrees Celsius.
Optimum pH is between 7-8 for the active carboxypeptidase-like domain 1, and 5-6.5 for domain 2.
Optimum pH is 8.0-8.2.
Optimum pH is 5.5-9.5.
Optimum pH is 6.5-10.0.
Optimum pH is approximately 8.0.
Optimum pH is 7-8.3.
Optimum temperature is 37-45 degrees Celsius.
Optimum pH is 7-8. At low pH values the enzyme is rapidly inactivated, whereas in basic medium inactivation is rather slow.
Optimum temperature is 55 degrees Celsius. Shows a drastic decrease in activity above the optimal temperature. Is stable for 15 hours at 22 degrees Celsius and for 0.5 hour at 45 degrees Celsius.
Optimum pH is 5.5-6. It retains about 40% of its activity at pH 4.5 and 7.5.
Optimum temperature is 65 degrees Celsius. The enzyme is stable up to 70 degrees Celsius and lost 70% of its activity at 75 degrees Celsius.
Optimum pH is 8.0. Retains about 40% activity at pH 5.5.
Optimum temperature is 37 degrees Celsius. Shows maximal activity between 30 and 40 degrees Celsius.
Optimum pH is 9.0 (PubMed:4889950). Optimum pH is 9.5 (PubMed:14087358).
Stable at acidic pHs.
Optimum pH is 6.7 when incubated for 5 minutes at 35 degrees Celsius. Active from pH 4.5 to 12.5 when incubated for 5 minutes at 35 degrees Celsius. Optimum pH is 7.0 when incubated for 24 hours at 4 degrees Celsius. Active from pH 5.0 to 10.5 when incubated for 24 hours at 4 degrees Celsius.
Stable up to 40 degrees Celsius, but activity is lost after 15 minutes incubation at 60 degrees Celsius.
Optimum pH is 6.5 with ONPG as substrate. Measured at 37 degrees Celsius and in 4 M KCl.
Optimum temperature is around 50-55 degrees Celsius in 4 M KCl and at pH 6.5. Partial activity (10-13% of maximum) at temperatures from 4-10 degrees Celsius.
Optimum temperature is 36-38 degrees Celsius in the absence of L7Ae, 48-50 degrees Celsius in presence of L7Ae.
Optimum pH is 5.5. Shows more than 75% of maximum activity from pH 5 to 10. Is still active at pH 12.
Optimum temperature is 50 degrees Celsius. Loses 100% activity after incubation for 15 minutes at 50 degrees Celsius.
Optimum temperature is 25 degrees Celsius. The ability to inhibit trypsin slowly decreases as the temperature increases to 70% inhibition at 100 degrees Celsius.
Optimum pH is between 5-5.5. Loses activity rapidly when pH is lowered from 5 to 4.
Optimum pH is 6.0. Stable in the pH range from 4.0 to 8.0.
Optimum temperature is 60 degrees Celsius. Around 53 percent activity is retained at 70 degrees Celsius. Stable from 30 to 60 degrees Celsius with maximum stability at 30 degrees Celsius.
High activity at 37 degrees Celsius. Little or no activity at 0 degrees Celsius.
Optimum pH is 4.5-5 (for chondroitin sulfate hydrolase activity).
Optimum pH is 6.3 to 6.5 for unwinding of a 56 bp substrate and DNA hairpin binding.
Optimum pH is 7.5 with myricetin as substrate (in the presence of S-adenosylmethionine).
Optimum pH is 5.6 for the phosphatase activity and 8.8 for the peroxidase activity.
Optimum temperature is 50 degrees Celsius. Stable at 57.5 degrees Celsius, pH 5.0 for 10 minutes, used to purify protein.
Optimum pH is 6.5. Active over a broad pH range.
Optimum pH is 6.5-7.2.
Optimum pH is near 7.5.
Optimum temperature is about 40 degrees Celsius.
Optimum pH is 7.5 for L-malate.
Optimum pH is 4.5 with CMC as substrate.
Optimum temperature is 50 degrees Celsius with CMC as substrate. Retains 50% of its activity at temperatures ranging from 30 to 60 degrees Celsius.
Optimum pH is between 6.0 and 7.0.
Optimum temperature is 7.4 degrees Celsius.
Optimum pH is between 6-8.5.
Optimum pH is >7.5.
Optimum pH is 6.5-7.5.
Optimum pH is 3.5 with 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) as substrate, 5.0-7.5 with guiacol as substrate, and 6.0-7.0 with syringaldazine as substrate.
Optimum pH is between 7.5 and 7.75.
Optimum pH is 4.0-5.0.
Optimum temperature is 30 degrees Celsius. Stable below 75 degrees Celsius, and retains 69% of the original activity at 80 degrees Celsius.
Optimum pH is 7.3 for the reductive deacylation of HMG-CoA (in the presence of 3 M KCl).
Optimum pH is 7.8-7.9.
Optimum pH is 3 and 7-8. Expressed as a fusion protein to increase stability.
Optimum temperature is 50 degrees Celsius. Expressed as a fusion protein to increase stability.
Optimum temperature is 52 degrees Celsius.
Optimum temperature is 51 degrees Celsius. However, enzyme stability is dramatically reduced at this temperature, incubation for 30 minutes at 31 and 49 degrees Celsius results in 61% and 17% residual activity, respectively. Incubation at 60 degrees Celsius leads to total inactivation of the enzyme.
Optimum pH is 7.5 with S-benzyl-N-acetyl-L-cysteine as substrate, 7.6 with N-acetyl-L-histidine as substrate, 7.6 with N-acetyl-L-tyrosine as substrate, and 7.7 with N-acetyl-L-phenylalanine as substrate.
Optimum pH is 9.4. Active from pH 7 to pH 11.
Optimum temperature is 55-65 degrees Celsius.
Optimum pH is 8 and 9 for the reductive amination and for the oxidative deamination, respectively.
The enzyme remains active when heat treated or when left at room temperature for several months but is inactivated by freezing.
Optimum pH is 8.4-9 (PubMed:14114846). Optimum pH is 8.4-9.2 (PubMed:20659527).
Optimum temperature is 80 degrees Celsius. Highly thermostable. Retains 80% activity after incubation at 90 degrees Celsius for 10 minutes.
Optimum pH is 8.0 (in the presence of 1 mM tetrahydrofolate).
Optimum pH is 6.7.
Optimally active at alkaline pHs.
Optimum pH is 5.4. Displays 50% of the optimal activity between pH values 4.0 and 6.2. A pH above 7 leads to a complete inhibition of the enzyme.
Optimum temperature is 35-45 degrees Celsius.
Optimum pH is between 10 and 11 for the oxidative deamination and between 7 and 7.5 for the reductive amination.
Thermostable up to 63 degrees Celsius.
Optimum temperature is 70 degrees Celsius with D-allulose as substrate.
Active between 21 and 40 degrees Celsius.
Optimum pH is 5.3-5.6 with 1,3-1,4-beta-glucan as substrate (at 35 degrees Celsius).
Optimum pH is 7-10. Displays little to no activity at low pH (pH 6.5 and below).
Optimum temperature is 40-50 degrees Celsius. Is active over a wide range of temperature (20-60 degrees Celsius). However, the enzyme is not active at 70 degrees Celsius.
Optimum pH is 8.0. Less active at pH 5.0 and pH 10.0.
Optimum temperature is 37 degrees Celsius. Less active at 25 degrees Celsius.
Optimum pH is 7.8. Active from pH 5.5 to 8.5.
Optimum pH is 4.0. Highly stable at acid pH. Retains 100% of its activity after 90 minutes incubation at pH 4.5 and 45 degrees Celsius, and 95% of the initial activity is found after 15 days incubation at room temperature and pH 4.5.
Optimum temperature is 40 degrees Celsius. Shows 98% and 51% of its maximum activity at 45 and 55 degrees Celsius, respectively.
Optimum pH is 6.8-7.5.
Optimum pH is 8.0-8.5. Active from pH 6.0 to 10.0.
Optimum pH is 3.5 for F420 reduction with NADPH, and 8.0 for NADP(+) reduction with F420H(2).
Optimum pH is 7.1-9 for the 3-deoxyglucosone dehydrogenase activity.
Optimum pH is 4.5-5.
Optimum pH is 5.5. Retain 85 percent of its activity in the pH range from 6.0 to 7.0 after a 12 h incubation at 25 degrees Celsius.
Optimum temperature is 58 degrees Celsius. The purified enzyme showed complete stability under 60 degrees Celsius.
Optimum pH is between 7 and 7.5. Approximately 30% of the maximal activities are still detected at pH 5 and pH 9.
Optimum pH is 5.0 with ONPG as substrate.
Optimum temperature is 70 degrees Celsius with ONPG as substrate. Stable for several months at 4 or -20 degrees Celsius in the presence of 10% glycerol. Activity increases continuously from 30 to 90 degrees Celsius and drops dramatically at higher temperatures. Retains 50% of its initial activity after 1 hour incubation at 70 degrees Celsius. Retains 90% and 50% of activity after 30 minutes of incubation at 70 and 80 degrees Celsius, respectively. The residual activity of the enzyme decreases to an undetectable level after heating for 30 minutes at 90 degrees Celsius. The enzyme still maintains significant activity at 30 degrees Celsius.
Optimim pH is 4.3. Active from pH 3 to 6.5.
Stable from 4 to 37 degrees Celsius. Activity is reduced to one third of the original level after incubation at 50 degrees Celsius for 30 minutes.
Optimum pH is close to 8 (PubMed:21085589). Activities at pH 7 and pH 9.5 are 57% and 70% of the maximal activity, respectively (PubMed:21085589, PubMed:21045009).
Optimum pH is 8.3.
Optimum temperature is 55 degrees Celsius. It retains only half of its original activity after a 30 minutes incubation period at 50 degrees Celsius.
Optimum pH is 9.4.
Optimum temperature is 45-55 degrees Celsius. Thermostable.
Loss of activity below 10 degrees Celsius.
Active from pH 4 to 10.
Optimum temperature is 28 degrees Celsius. Active from 4 to 40 degrees Celsius.
Optimum pH is 6-7 for hemagglutinating activity. Activity drops rapidly at lower or higher pH.
Thermostable. Retains hemagglutinating activity after incubation at 60 degrees Celsius for 1 hour. At higher temperatures activity drops drastically.
Optimum pH is 7.0 for PMI activity, and 7.6 for GMP activity.
Optimum pH is 9.5 for oxidative deamination, and 9.0 for reductive amination. Retains more than 80% of its activity after incubation for 30 minutes at pH 4.5-9.5.
Optimum temperature is 90 degrees Celsius for oxidative deamination. Retains full activity after incubation for 10 minutes at temperatures up to 90 degrees Celsius.
Optimum temperature is 20-30 degrees Celsius.
Optimum pH is 9.8.
Optimum pH is 6.0-10.5.
Optimum temperature is 4-60 degrees Celsius.
Optimum pH is between 7 and 9 at 30 degrees Celsius. The enzyme is stable from 7 to 11 at 30 degrees Celsius.
Optimum temperature is around 60 degrees Celsius. The enzyme is stable up to 60 degrees Celsius for 10 minutes.
Optimum pH is 7.8-9.5.
Optimum pH is 6.6-6.8.
Optimum temperature is about 37 degrees Celsius.
Optimum pH is 4.0 or less.
Optimum temperature is 30-42 degrees Celsius. Partially active up to 65 degrees Celsius (PubMed:9593741).
Optimum temperature is between 30 and 40 degrees Celsius.
Optimum pH is 6.8 in the absence of calcium ions. Optimum pH is 5.6-8.5 in the presence of calcium ions.
The activity is markedly reduced above 40 degrees Celsius. Calcium ions increases the thermal stability by 10 degrees Celsius.
Optimum temperature is 43 degrees Celsius.
Optimum pH is 4-5 at 40 degrees Celsius for chitinase activity.
Optimum temperature is 76 degrees Celsius.
Optimum pH for hydrolysis of beta-cyclodextrin is approximately 7.5 (PubMed:9606956, PubMed:16536613). Optimum pH for hydrolysis of soluble starch is 6.0 and 6.0-7.0 for hydrolysis of pullulan (PubMed:9606956).
Optimum temperature is 50 degrees Celsius for hydrolysis of beta-cyclodextrin (PubMed:9606956, PubMed:16536613). It is elevated from 40 to 50 degrees Celsius by addition of Ca(2+), and the thermal activity is retained more than 80% at 60 degrees Celsius in the presence of Ca(2+). The optimum temperature is between 40-50 degrees Celsius for hydrolysis of soluble starch, and it is 50 degrees Celsius for hydrolysis of pullulan (PubMed:9606956).
Optimum pH is 7.2-7.5.
Optimum pH is 6.4-7.0.
Unstable below pH 8, unless zinc is present.
Optimum pH is 8.3 for the oxidation of 2-hydroxymuconic semialdehyde, and 9.6 for that of benzaldehyde.
Optimum pH is 7.0-11.0.
Optimum pH is 8.0 with pyridoxal as substrate (9.5 with L-fucose as substrate).
Optimum pH is 5.5 for the reduction of GGPP to the hexahydro-product H(6)GGPP.
Optimum temperature is 55 degrees Celsius for the reduction of GGPP to the hexahydro-product H(6)GGPP.
Optimum pH is 5.7-6.3 for the forward reaction and 7.0-7.5 for the reverse reaction.
Optimum pH is 8.0 (Ref.1). Stable between pH 7.5 and 8.5 (Ref.2).
Rapidly inactivated at temperatures above 40 degrees Celsius.
Optimum pH is 8.0 (with pNP-butyrate as substrate).
Optimum temperature is 96 degrees Celsius. High thermostability.
Optimum pH is 6.0-8.0 (PubMed:34277586). Activities drop at pH values below 5.5 and above 9.0 (PubMed:34277586).
Optimum temperature is 40-50 degrees Celsius (PubMed:34277586). Displays high thermostability with 90% of remaining activity after 2 days at 30 degrees Celsius and a half-life of 18 hours at 60 degrees Celsius (PubMed:34277586).
Optimum pH is 8.5 (with palmitate as substrate).
Optimum pH is near 5.5.
Optimum pH is 4.5-6.0. Active from pH 4 to 7.5. Stable from pH 4 to 11.
Optimum temperature is 44 degrees Celsius. Active from 30 to 55 degrees Celsius. Thermostable for 30 minutes up to 50 degrees Celsius.
Optimum temperature is 55 degrees Celsius, activity was detected from 4 to 95 degrees Celsius.
Optimum temperature is around 95 degrees Celsius. Shows >70% of its maximal activity in the pH range of 5-9. Displays a relatively high thermostability with a half-life of 1 hour at 100 degrees Celsius.
The direction of reaction is pH dependent in vitro. At pH 7.3, clearly the reduction reaction is preferred with an order of magnitude higher velocity than the oxidation reaction. At pH 8.7, the velocities of the oxidation and reduction reaction are comparable. At pH 9.9 the velocity of the reduction reaction is about 60% of oxidation reaction.
Optimum pH is 6.1.
Optimum temperature is 100 degrees Celsius. Highly thermostable.
Optimum temperature is 30 degrees Celsius for highest reaction speed, and 25 degrees Celsius to obtain highest molecular weight of product chondroitin.
Heating at 60 degrees Celsius inactivates the enzyme. Heating at 60 degrees Celsius in the presence of substrate prevents inactivation.
Optimum pH is 6 for the reverse reaction (reduction) with activity decreasing sharply towards pH 4.5 and pH 7.5. Optimum pH is 9.5 for the forward reaction (oxidation) with greatly reduced activity at pH 6.
Optimum pH is 6.1-6.5.
Maximum activity at 30 degrees Celsius. Labile above 40 degrees Celsius.
Optimum pH is 7.0 for indolepyruvate reduction. Optimum pH is 8.5 for indolelactate oxidation.
Optimum temperature is 120 degrees Celsius. Thermostable up to 133 degrees Celsius.
Optimum pH is 7.1-7.3.
The half-life at 58 degrees Celsius is less than 3 minutes.
Unthreads upon heat treatment.
Hemolytic activity is observed from 6 to 37 degrees Celsius for mammalian erythrocytes.
Optimum pH is 4.5 with 2,6-dimethoxyphenol as substrate. Retains more than 50% of its activity after 4 hours at pH 2.5.
Thermostable. Retains 50%-90% of its activity after heating for 2 hours at 60 degrees Celsius, while no decrease in activity is observed within the same time at 30 or 40 degrees Celsius.
Optimum pH is 8.0. Active from pH 6.5 to 9.5.
Stable up to about 51 degrees Celsius.
Optimum temperature is 47 degrees Celsius.
Optimum pH is 4.5. More than 70% of the optimum activity remains at pH between 3 and 7.
Optimum temperature is 90 degrees Celsius. It exhibits more than half of the maximum activity at temperatures ranging from 70 to 100 degrees Celsius.
Stable between pH 5.0 and pH 8.7. Activity is lost at pH 3.0 and above pH 9.5.
Thermostable, retains activity after heating at 100 degrees Celsius for 5 minutes.
Optimum pH is between 8 and 8.5, with a dramatic drop of activity (55%) at pH 9. Less than 10% activity is observed a pH 6, and half the maximal activity is measured at pH 7.
Optimum temperature is 37 degrees Celsius. The enzyme is extremely labile, and the activity is totally lost within 48 h at 4 degrees Celsius.
Optimum pH is 5.5 or less for Arg-Agm exchange, which slows dramatically as pH rises and is almost undetectable at pH 7.0 and higher.
Optimum pH is 8.2 for guanosine kinase activity. Optimum pH is 6.9 for inosine kinase activity.
Optimum temperature is 38 degrees Celsius for guanosine kinase activity and between 26 and 39 degrees Celsius for inosine kinase activity.
Thermostable (PubMed:33431675). Retains antibacterial activity against M.luteus after heat treatment at 60 degrees Celsius for 10 minutes (PubMed:33431675).
Optimum pH is 7.0-7.2 for nucleotidase activity.
Optimum temperature is 105 degrees Celsius for ssDNA nicking, 75 degrees Celsius for nucleotidyltransferase activity on a ssDNA substrate. Optimum temperature for ssDNA closing is substrate dependent, being 55 and 75 degrees Celsius for the 2 substrates tested. Topoisomerase activity is only seen between 55 and 75 degrees Celsius.
Optimum pH is 6. Highly active in the pH range 5-7. Is stable at pH values from 4 to 8. At pH values above 8 and below 4 the enzyme is rapidly inactivated.
Optimum temperature is 40-45 degrees Celsius. Is stable at temperatures from 4 to 37 degrees Celsius. During 30 minutes of incubation at 40 degrees Celsius, about 20 to 30% of the activity is lost, and at 50 degrees Celsius only 20% of the activity is retained.
Optimum pH is 9.0, active between pH 7.5 and 10.5.
Optimum pH to bind cytokinin is about 8.5 at 0 degrees Celsius.
Cytokinin-binding is stable at 0 degrees Celsius but transient at 37 degrees Celsius.
Optimum pH is 9.5. The activity declines strongly above pH 10.
Optimum temperature is 85 degrees Celsius. 70% of maximal activity at 72 and 95 degrees Celsius.
Optimum pH is 5-6.
Optimum temperature is 25-37 degrees Celsius.
Optimum pH is 6.5-9.5 for phosphonoacetaldehyde formation, and 7.5-8.5 for 2-aminoethyl phosphonate formation.
Optimum temperature is 40 degrees Celsius. Above this temperature, the enzyme activity decreases significantly, exhibiting 66% of the maximum activity at 50 degrees Celsius. Below 40 degrees Celsius, the enzyme activity decreases with decreasing temperature, exhibiting 15% of the maximum activity at 25 degrees Celsius. The half-lives of the enzyme at 35, 40, 45, 50, and 55 degrees Celsius are 17.6, 15.0, 12.5, 6.1, and 1.5 hours, respectively.
Optimum pH is 8.5 for MPP(+) uptake (PubMed:9830022). Optimum pH is 8.5 for TEA uptake (PubMed:9632645).
Optimum pH is 7.4 and 6.0 for the oxidation of ethanol and reduction of acetone, respectively.
Is a very thermostable, cold-adapted enzyme. Is active from 10 to at least 53 degrees Celsius and unfolds at 89 degrees Celsius.
Optimum pH is between 8.0 and 9.0 with activity decreasing sharply below 8.0.
Optimum pH is 7.0 with ONPG as substrate. Retains 60% of its activity between pH 6.0 and 8.0.
Optimum temperature is 37 degrees Celsius with ONPG as substrate. Retains 60% of its activity between 20 and 45 degrees Celsius.
Optimum pH is 7.4 for the adenylylsulfate reductase.
Optimum pH is acidic (5.5-6.0) for FMN phosphatase activity and basic for riboflavin kinase activity.
Optimum pH is 6.0. It retains more than 75% activity over a pH range from 4 to 7.
Optimum temperature is 73 degrees Celsius. It is fully heat-stable (up to 10 hours) and retains activity at temperatures up to 90 degrees Celsius. The enzyme loses its activity only after 90 minutes when incubated at 95 degrees Celsius.
Optimum pH is 7.0-7.5 and pH 9.6 for the reaction in the direction of SIH synthesis. Shows no activity at either pH 6.5 or pH 11.5 in the synthetic direction. The activity of SIHH in the hydrolysis direction shows essentially the same activity over the pH range 6.5-11.5.
Optimum temperature is about 70 degrees Celsius.
Stable in a pH range between 5.0 and 8.5 at 40 degrees Celsius for 30 min.
Optimum temperature is 28 degrees Celsius.
Optimum pH is 7.4-8.0.
Optimum pH is 2.5.
Optimum temperature is 75 degrees Celsius. The enzyme is heat stable.
Optimum pH is 7.2-7.7 (PubMed:7400101). Optimum pH is 7.6 (PubMed:23204427).
10 minutes at 100 degrees Celsius abolishes completely the activity.
Optimum pH is 7.0-7.6.
Optimum pH is 7.7-8.5.
Heat stable and fully active up to 70 degrees Celsius.
Optimum pH is 6.5 with estrone 3-sulfate, taurocholate and L-thyroxine (T4) as substrates.
Optimum temperature is from 20 to 40 degrees Celsius. Retains full activity up to 50 degrees Celsius (PubMed:16212940). Retains full activity up to 45 degrees Celsius (for six-histidine-tag protein) (PubMed:17126561).
Optimum temperature is 35 degrees Celsius. Half of the activity is lost after treatment at 40 degrees Celsius for 15 minutes.
Optimum pH is 10.0 for ThTP synthesis.
Optimum temperature is 37 degrees Celsius for ThTP synthesis.
Optimum pH is 8.7.
Exhibits stability over a wide range of pH (5.0-9.0).
Stable up to 40 degrees Celsius.
Activity is lost under pH 5 but not affected up to pH 10.
Optimum pH is around 8. The enzyme is stable in a pH range of 9-10.
Optimum temperature is around 44 degrees Celsius. The enzyme is stable up to 50 degrees Celsius.
Optimum pH is 12.
The empty encapsulin nanocompartment is stable until 86.6 degrees Celsius, when loaded with cargo protein is stable until 88.9 degrees Celsius and when grown in high-iron conditions is stable until 91.8 degrees Celsius.
Optimum pH is about 7.0.
Optimum temperature is about 42 degrees Celsius.
Optimum pH is 6.1-8.5.
Optimum pH is above 9.0 for esterase activity.
Optimum temperature is 70 degrees Celsius for esterase activity.
Optimum pH is 5.5-8.0.
Optimum pH is 7.9 in phosphate buffer.
Optimum temperature is 22 degrees Celsius.
Optimum temperature is 90 degrees Celsius. Half-life in the presence of 0.6 mM CaCl(2) is 110 minutes at 95 degrees Celsius and 15 minutes at 100 degrees Celsius. Half-life in the absence of CaCl(2) is 5 minutes at 90 degrees Celsius.
Optimum pH is 3-4.
Preincubation of the enzyme for 10 min at temperatures above 35 degrees Celsius decreases acyltransferase activity subsequently measured at 30 degrees Celsius. Acyltransferase activity is reduced by approximately 60% following 10 min preincubation at47 degrees Celsius.
Optimum pH is 4.7.
Optimum pH is 8.0 for NADP reduction with F420H(2). Optimum pH is 5.5 for F420 reduction with NADPH.
Optimum temperature is 80 degrees Celsius. Is highly thermostable.
Optimum temperature is 60 degrees Celsius. Remains stable after 15 minutes at 60 degrees Celsius.
Optimum pH is 5.0. Active between pH 4.0 and 10.0.
Optimum temperature is 40 degrees Celsius. Stable below 50 degrees Celsius.
Optimum pH is 6.5-8.5 (PubMed:1655714). Optimum pH is 8.0-9.0 (PubMed:15716459).
Optimum pH is around pH 9 (with saturating concentrations of dGuo and UTP). At pH 7.5 and 11.5 more than 80% of maximal activity is still observed. At pH 6.0, 60% activity remains, whereas the enzyme is completely inactive below pH 5.6.
Optimum temperature is 21 degrees Celsius.
Optimum pH is 6.5 in 2.0 M NaCl. Stable over a range of pH 5.7-9.2.
Optimum temperature is 45 degrees Celsius in 2.0 M NaCl and at pH 6.5. Relatively stable up to 55 degrees Celsius.
Optimum pH is 5.4.
Optimum pH is 7.5-8.0 at 20 degrees Celsius, and 8.0-8.5 at 25 degrees Celsius in the presence of NAD(+).
Optimum pH is 4.5-5.5 for the polymerization reaction.
Optimum temperature is 50-56 degrees Celsius for the polymerization reaction.
Optimum pH is 8 for the hydrolysis of Gly-Phe-pNA.
Optimum temperature is approximately 30 degrees Celsius for the hydrolysis of Gly-Phe-pNA. Stable at a temperature below 20 degrees Celsius for 30 minutes.
The enzyme is active over the temperature range 20-60 degrees Celsius, showing an increase in specific activity with temperature.
Optimum pH is 8.0. Active from pH 4.0 to 10.0. In unbuffered solutions, the dodecameric complex is active at pH3.0.
Optimum pH is 5.5 for fucose transport.
Is more active at pH 8.0 rather than at pH 7.0 or pH 6.0.
Optimum pH is 8.0. At pH 9.0 or above, only 30% activity is observed.
Optimum temperature is 37 degrees Celsius. The activity decreases slowly when temperatures are reduced from 37 degrees Celsius while the drop in activity is relatively more rapid when temperatures are raised above 37 degrees Celsius. In this case, the activity reduces to almost 30% at 52 degrees Celsius.
Optimum pH is 6-8 (with homotetramer).
Optimum pH is 7.3.
Optimum pH is 6-7 for the electron donation reaction to monodehydroascorbate reaction (PubMed:23641721). Optimum pH is around 6.5 for re-reduction reaction process of the oxidized heme with ascorbate (PubMed:23641721).
Optimum pH is 5.5-6.0.
Optimum pH is 4.5-5.5.
Optimum pH is 7 with (9Z,12Z)-octadecadienoate as substrate.
Optimum pH is 6.5. Activity remains high up to pH 9.0.
Transformation of DCA is more efficient at pH 10 than pH 7.
Optimum temperature is 45 degrees Celsius. Thermostable. Is half-inactivated at 52 degrees Celsius.
Optimum pH is 10 (PubMed:11029590). The activity decreases strongly above pH 10.5 or below pH 6.5. The enzyme is remarkably stable at alkaline pH, showing maximum stability at pH 12 and retaining more than 65% of its activity after 24 hours at pH 13 (PubMed:8396026).
Optimum temperature is 35 degrees Celsius. Stable for at least 30 minutes at 40 degrees Celsius. Virtually no activity remains after 30 minutes at 55 degrees Celsius.
Optimum pH is 3.0-4.0.
Optimum pH is 8.3. High activity between pH 7.5 and 8.6, rapidly loses activity below pH 7.0. Loss of activity below pH 6.3 is irreversible.
Optimum pH is 7.0-9.5.
Optimum temperature is 38 degrees Celsius.
Optimum pH is 4.7-5.7.
Optimum temperature is 81 degrees Celsius. Thermostable up to 100.8 degrees Celsius. Poorly active at 25 degrees Celsius. Enzymatic activity is increased 4-fold by temperature increase from 25 to 45 degrees Celsius and more than 9-fold at 98 degrees Celsius.
Optimum pH is 6.4 for urate transport (PubMed:35462902). Optimum pH is 5.0 for p-aminohippurate transport (PubMed:18411268).
Optimum pH is 8 with RNA and ssDNA as substrates.
Optimum pH is 6. The enzyme retains full activity at pH values from 5.5 to 7.5. After exposure to pH 4.5, the activity is reduced by 40%, and no residual activity is detected at pH values below 4.5 or above 7.5.
Optimum temperature is 45 degrees Celsius. The enzymatic activity decreases significantly at temperatures below 35 degrees Celsius and above 60 degrees Celsius, although considerable levels of activity are still detected at 20 and 70 degrees Celsius.
Optimum pH is 8. It retains a high specific activity over a broad pH range.
Optimum pH is 8-9. Active from pH 5.5 to 9.3.
Optimum pH is 6.9.
Optimum pH is 3.7. Active within a narrow range of acidic pH values (pH 3.3 to 4.0).
Optimum temperature is 65 degrees Celsius. At 30 degrees Celsius activity is only 7% of the optimal value and at 80 degrees Celsius, the enzyme is totally inactive.
Optimum pH is 7.5 (PubMed:19574214). Active at pH 5.5-7.5 (PubMed:19574214).
Optimum pH is 6.5 for Pyr2C reduction and 10.5 for L-proline oxidation.
Optimum pH is 3.5-5.0 with the oligoxyloglucan heptasaccharide XXXG as a substrate. Over 90% activity is retained between pH 4.8 and 8.0.
Optimum temperature is 50-60 degrees Celsius with the oligoxyloglucan heptasaccharide XXXG as a substrate.
Optimum pH is 8.5 to 9.0 for the hydrolysis of Gly-Phe-pNA.
Optimum temperature is between 35 and 40 degrees Celsius for the hydrolysis of Gly-Phe-pNA. Stable at a temperature below 20 degrees Celsius for 30 minutes.
Optimum pH is between 7-8 (at 40 degrees Celsius).
Optimum temperature is between 40 and 55 degrees Celsius (at pH 7). Activity decreases at 60 degrees Celsius.
Active at acidic pHs. Inactive above pH 7.
Optimum pH is 11.0. Stable from pH 7.0 to 11.0.
Optimum temperature is 69 degrees Celsius. Thermostable, retains 100% of activity after 2 hours incubation at 65 degrees Celsius.
Optimum pH is 7.0-8.5. Activity decreases rapidly at basic or acidic pH and is virtually absent at pH 12 and pH 3.5, respectively.
Optimum pH is 8.2-8.7.
Optimum pH is between 8.5 and 9.
Optimum pH is 5.5. The degradation is efficient at pH values between 4.5 and 6.
Optimum pH is around 8.3.
Optimum pH is 6.3 for the reverse reaction.
Optimum pH is 9.0. Exhibits antiport activity at a wide pH range of 6.5 to 9.5.
Optimum pH is 8-10.
Optimum pH is 7.6-8.
Optimum temperature is 50-55 degrees Celsius at pH 8 and 63 degrees Celsius at pH 7.6.
Optimum pH is 4.2.
Optimum pH is between 6.5-7.5.
Optimum pH is 5.5-7.5.
Is one of the thermostable restriction enzymes that remain active after 20 to 30 cycles of thermal PCR cycling.
Optimum temperature is between 45 and 50 degrees Celsius.
Active from pH 6.5 to 10.
Optimum temperature is greater than 90 degrees Celsius. Activity increases with increasing temperature from 30 degrees Celsius to 90 degrees Celsius. Has a half-life of 6 hours at 95 degrees Celsius.
Optimum pH is 7.8-8.2.
Optimum pH is 7.0. Active from pH 4.0-8.0.
Optimum pH is 10.
Is active against Ala-Phe-pNA over a broad pH range, from neutral to basic pH (6.5-9.0).
Optimum temperature is 43 degrees Celsius, using Ala-Phe-pNA as substrate.
Optimum temperature is 65 degrees Celsius. Very thermounstable, complete loss of activity after 15 minutes at 60 degrees Celsius.
Optimum pH is 8.0 for G6PD activity.
Optimum pH is 8-9.5.
Optimum pH is 6.6-7.2.
Optimum pH is 4.5-7.5.
Enzyme activity is retained almost fully under 40 degrees Celsius and completely destroyed at 70 degrees Celsius. At 60 degrees Celsius the activity was 15-30% compared to the untreated enzyme.
Optimum pH for enzymatic activity is substrate-dependent, with optimal hyaluronate degradation at pH 5, poly-beta-D-glucuronate degradation at pH 7, and alginate degradation at pH 9.
Optimum pH is 3.0 with ABTS as substrate, activity decreases sharply as pH increases, and no activity is seen at pH 7.0. Optimum pH is 4.0 with syringic acid as substrate, 5.0 with caffeic acid, syringaldazine, vanillic acid or catechol as substrate, and 6.0 with levodihydroxyphenylalanine as substrate. Inactive after 3 hours incubation at pH 2.0, retains 20% of its activity after 24 hours at pH 3.0 and 90% of its activity after 24 hours at pH 9.0.
Optimum temperature is 60 degrees Celsius. Shows 50% of its maximal activity at 20 degrees Celsius. Has a half-life of 53 minutes at 50 degrees Celsius, 23 minutes at 60 degrees Celsius, and less than 2 minutes at 70 degrees Celsius.
Optimum pH is 8.0 (PubMed:10759857). Optimum pH is 8.5 for glycerol phosphorylation and 7.0 for ATP formation (PubMed:11154065).
Optimum temperature is 60 degrees Celsius. Activity is rapidly lost above 70 degrees Celsius.
Optimum pH is 7-8 (PubMed:1416986, PubMed:15703908). Active from pH 6 to pH 8.5 (PubMed:15703908, PubMed:18587571).
Optimum temperature is 42-55 degrees Celsius.
Optimum temperature is 65 degrees Celsius. Highly thermostable.
Optimum pH is 6.5 in sodium-acetate at 50 degrees Celsius for poly(P)-dependent kinase activity and optimum pH is 8 in Tris-HCl at 50 degrees Celsius for ATP-dependent kinase activity.
Optimum pH is 3.0.
Optimum pH is 6.7, using Lys-Ala-MCA as substrate.
Optimum pH is 8.0. Active from pH 7.0 to 9.0.
Optimum temperature is 25 degrees Celsius. Stable at temperatures lower than 40 degrees Celsius.
Optimum pH is 7-7.5. Active from pH 5.25 to pH 9.
Optimum temperature is 37 degrees Celsius (PubMed:27023914). However, has substantial activity at 25 degrees Celsius (PubMed:27023914).
Stable between pH 4 and 10.
Thermostable. Retains antifungal activity between 4 degrees Celsius and 60 degrees Celsius. Activity reduced after 30 min at 80 degrees Celsius.
Optimum pH is about 9.0 with L-kynurenine as substrate, and about 8.5 with DL-3-hydroxykynurenine as substrate.
Still active at temperatures close to 0 degrees Celsius. Thermolabile.
Optimum pH is 7.0-7.5 for phosphorolysis, and 6.0-7.0 for synthetic reaction.
Optimum pH is 9. No activity between pH 3-6.
Optimum temperature is 45 degrees Celsius. Activity is about 80% of the maximum activity at 35 and 50 degrees Celsius.
Optimum pH is 7.6 for isoform A with Trx-2 and NADPH as substrates.
Optimum pH is 3.7. Active within a narrow range of acidic pH values (pH 3.2 to 3.9).
Optimum temperature is 50-55 degrees Celsius. Stable up to 50 degrees Celsius, inactivated after incubation at 60 degrees Celsius for 30 minutes. Activity decreases below 50 degrees Celsius, with 20% of activity retained at 25 degrees Celsius.
Stable below pH 6.
Stable below 60 degrees Celsius. Activity decreases sharply at temperatures above 60 degrees Celsius.
Optimum pH is 7.3 with 4'-nitrophenyl beta-D-glucopyranoside as substrate. Precipitates below pH 6 and stable from pH 6.8 to 8.4.
Optimum pH is 5.0 for the peroxidase reaction and 6-6.5 for the catalase reaction.
Optimum pH is 6.5-7.5. Significant phosphatase activity is observed between pH 5.5 and 8.0.
Optimum pH is 8 (PubMed:23604968). Optimum pH is 6.0-6.5 (PubMed:15638529). Optimum pH is 7.5 (PubMed:20816933).
Optimum temperature is 25-50 degrees Celsius (PubMed:24728714). Optimum temperature is 55 degrees Celsius (PubMed:23604968). Optimum temperature is 60 degrees Celsius (PubMed:20816933). Optimum temperature is 65 degrees Celsius (PubMed:15638529, PubMed:31690819, PubMed:32269349).
Optimum pH is 7.2 for the forward reaction and 8.6 for the revesre reaction.
Optimum pH is 6.8 for reduction (forward reaction) of codeinone to codeine and 9.0 for the oxidation (reverse reaction) of codeine to codeinone.
Optimum pH is 6.5 - 7.5.
Resistant to heat. Retains its alpha-helical fold after heating at 90 degrees Celsius and subsequent cooling to 20 degrees Celsius.
Optimum pH is 7.4-8.5.
Optimum temperature is 55 degrees Celsius. Unstable at temperatures above 45 degrees Celsius.
Optimum pH is 9-9.5 for the forward reaction (oxidation) and 7.0 for the reverse reaction (reduction).
Optimum temperature is 35 degrees Celsius for the reaction in both directions.
Extremely thermostable. Has a melting temperature of 119.9 degrees Celsius.
Optimum pH is 7.5 for recombinant LGox (PubMed:27999974). Optimum pH is 7-8.5 for proteinase K-treated recombinant LGox (PubMed:27999974).
Optimum temperature is 40 degrees Celsius for both recombinant LGox and proteinase K-treated recombinant LGox.
Optimum pH is 7 (at 55 degrees Celsius).
Optimum pH is between 7 and 8.
Extremely thermostable, being unaffected after incubation for 24 hours at 95 degrees Celsius.
Optimum temperature is 93 degrees Celsius.
Optimum pH is 6.4-7.2.
Thermostable from 20 to 43 degrees Celsius.
Optimum temperature is 50 degrees Celsius. Half-life of the enzyme activity is 55 minutes at 70 degrees Celsius.
Optimum pH is 4.85.
Optimum pH is 8.1 with spermidine as substrate. Optimum pH is 8.5 with glutathionylspermidine as substrate.
Thermal denaturation midpoint (Tm) is 54.4 degrees Celsius.
Optimum pH is 6.75.
Optimum pH is 6.5 for the native enzyme and 7.0-7.5 for the PPD-GST recombinant enzyme.
Optimum pH is 2.5 with hemoglobin as substrate.
Optimum pH is between 6.
Optimum pH is 7.41 for the native enzyme.
Optimum pH is 8.8-9.
Optimum pH is 9.0 for Na(+)/H(+) and Li(+)/H(+) antiport activities. Optimum pH is 8.5 for K(+)/H(+) antiport activity.
Optimum pH is 8.0-8.5. Exhibits at least 60% of its optimal activity over a rather broad pH range, from 5 to 7.
Optimum temperature is 70-75 degrees Celsius. Highly thermostable. Retains 100% of its activity after 24 hours of incubation at 70 degrees Celsius. At 90 and 100 degrees Celsius, the enzyme shows a half-life of 15 and 5 min, respectively.
Optimum pH is dependent on the substrate: optimum pH is 5-8 with phosphorylcholine, 6 with phosphorylethanolamine and 5 with pNPP.
Optimum temperature is between 85 and 90 degrees Celsius. It is stable at high temperatures, however at 90 degrees Celsius the stability of the enzyme is only moderate and 50% of the activity is lost after 30 minutes incubation. At 65 degrees Celsius, the specific enzymatic activity is 5-fold lower than the maximum value.
Optimum pH is 6 with cyclic PET trimers as substrate (at 60 degrees Celsius).
Optimum temperature is 60 degrees Celsius with cyclic PET trimers as substrate.
Optimum pH is 9.5 for the oxidation reaction, and 6.5 for the reduction reaction.
Optimum temperature is 28-33 degrees Celsius. Active from 22 to 37 degrees Celsius.
Optimum pH is 7.4-7.8 for ATPase activity.
Optimum temperature is 37 degrees Celsius for ATPase activity.
MptA loses 27% activity when it incubates for 10 min at 80 degrees Celsius, 60% activity at 90 degrees Celsius, and 89% activity at 100 degrees Celsius.
Optimum temperature is between 45 and 65 degrees Celsius. The activity of the enzyme at 80 degrees Celsius is half of the maximum.
DapF is almost 50% more active at 30 degrees Celsius than at 25 degrees Celsius. At 37 degrees Celsius the activity is slightly less than at 30 degrees Celsius.
Optimum temperature is 25-30 degrees Celsius.
Optimum pH is 7.4-7.8.
Optimum pH is between 7 and 9. The enzyme is active between pH 6 and 11.
Optimum temperature is 60 degrees Celsius. The enzyme is active between 20 and 65 degrees Celsius.
Optimum pH is 4.8.
Activity is relatively constant from pH 7.5 to 9.0. Below pH 7.5, activity decreases with pH.
Optimum pH is 7.2-7.4.
Optimum pH is 6.8. Exhibits above 80% of its maximal activity in the pH range 6.0-8.0 using ONPG as substrate at 20 degrees Celsius.
Optimum temperature is 20 degrees Celsius. Lowering or raising the temperature from 20 degrees Celsius results in reduction of activity when ONPG is used as substrate. Rather stable at or below 25 degrees Celsius, but loses 58% of activity after 30 minutes at 40 degrees Celsius. Loses all activity in only 10 minutes at 45 degrees Celsius. Exhibits 27% of maximal activity even at 0 degrees Celsius, but enzyme activity decreases with a further increase in temperature until it is undetectable above 50 degrees Celsius.
Optimum pH is 8.0-9.5.
Optimum pH is 7.1 for deoxyinosine as substrate (PubMed:235429). Optimum pH is 7.5 for inosine as substrate (PubMed:235429). Optimum pH is 8.2 for hypoxanthine as substrate (PubMed:235429).
Optimum pH is 10.3. At pH 9.0 and 11.0, the activity is 35% and only 6%, respectively, of the optimal level.
Optimum temperature is 75 degrees Celsius. Activity declines at temperatures from 75 to 80 degrees Celsius.
Optimum pH is 4-6.
Optimum pH is about 8.5.
Optimum temperature is 4 degrees Celsius. It does not grow at temperatures higher than 25 degrees Celsius.
Optimum pH is 7.5-8. Active from pH 7 to 9.
The catalytic activity of the enzyme increases up to 100 degrees Celsius. Thermostable, has a half-life of 11 minutes at 100 degrees Celsius, 36 minutes at 90 degrees Celsius and 100 minutes at 80 degrees Celsius.
Optimum pH is 10-11. Inactive at pH 4.5.
Optimum temperature is 75 degrees Celsius. Thermostable.
Optimum temperature is 37 degrees Celsius. Is not stable even stored at -80 degrees Celsius. About 50% of the activity is lost when stored at 4 degrees Celsius for 24 hours.
Optimum pH is 7.5-8.5. Inactive at or below pH 5.0.
Optimum pH is 9 in assays using artificial electron acceptors (PubMed:3144289). However, in a system with homogenous QEDH, cytochrome c550 and a membrane fraction of P.aeruginosa, electron transport from ethanol to O(2) is observed at the more physiological conditions of pH 7, and the system is inactive at pH 9 (PubMed:8380982).
Optimum pH is 8.2-8.8.
Optimum pH is 5.9. The enzyme is most stable in the pH range of 4.5 to 12.0, with 90% of the initial activity being retained even at pH 12.0.
Optimum temperature is 35 degrees Celsius. The enzyme is most stable from 10 to 50 degrees Celsius.
Optimum pH is 9 for agmatine and putrescine uptake.
Optimum pH is 6.0. At pH 4.0 the arabinosidase retains about 8% of activity.
Optimum temperature is 60 degrees Celsius. At 80 degrees Celsius retains about 2% of activity.
Optimum pH is 10.0 for gluconate oxidation. Optimum pH is 5.0 for 5-ketogluconate reduction.
Optimum pH is 8.0 (in the presence 30 mM Mg(2+)) and 7.5 (in the presence of 3 mM Mn(2+)).
Optimum pH is 5.5, and 50% of activity is found at pH 4.5 and 7.5.
Optimum temperature is 65 degrees Celsius. It shows high thermostability. At 70 degrees Celsius, the enzyme does not lose activity upon incubation for 2 hours. The half-lives of the enzyme at 80 degrees Celsius and 90 degrees Celsius are 20 minutes and 15 minutes, respectively.
Optimum pH is 5.5 with pNPP as substrate.
Optimum pH is 8. No activity is detected at pH 4.0-5.0. At higher pH values, it shows rapid decrease in activity, although 50% of the relative activity is still detected at pH 9.0. It retains much residual activity after 30 minutes incubation at pH 4.0-8.0, indicating that CrtC is stable in both slightly alkaline and acid environments.
Optimum temperature is 30 degrees Celsius. Enzyme activity is significantly lower at 20 degrees Celsius and 40 degrees Celsius.
Hyperthermostable. Remains fully active after 2 hours of incubation at 80 degrees Celsius.
Optimum pH is 7 (at 37 degrees Celsius). Is active between pH 4.0 and 10.0.
Optimum temperature is 45 degrees Celsius. Is active between 20 and 50 degrees Celsius.
Activity increases exponentially up to 100 degrees Celsius, the highest temperature tested.
Optimum pH is mildly basic for conversion of the glutaminyl substrates and shifted to acidic for cyclization of glutamic acid.
Optimum temperature is 94 degrees Celsius. Very thermostable, but rapidly inactivated above 94 degrees Celsius. Most of the activity is retained for 5 days at 50 degrees Celsius, and approximately 70% of the activity is retained after 5 days at 70 degrees Celsius. 52% of the activity is still retained after 50 hours at 90 degrees Celsius, but completely inactivated after 5 days at 90 degrees Celsius.
Stable in the pH range 4.0-12.0. Incubation at a pH below 4.0 rapidly decreases inhibitory activity.
Stable at temperatures up to 70 degrees Celsius. Incubation at temperatures above 70 degrees Celsius rapidly decreases inhibitory activity.
Optimum pH is7.0.
Optimum temperature is 85 degrees Celsius. Requires a high salt concentration for optimal thermostability at 90 degrees Celsius.
Optimum temperature is 20-37 degrees Celsius.
Optimum pH is 6.8-7.2 with NADPH as substrate and 6.8 with NADH as substrate.
Optimum temperature is 80 degrees Celsius for chromate reductase activity.
Optimum pH is 6-8 with glucose as substrate (PubMed:7742312). Optimum pH is 9-10 with glucosamine as substrate (PubMed:7742312).
Optimum pH is 6.5 and 7.5.
Optimum pH is 8.5 (PubMed:19931315). Optimum pH is 7-8 for the cleavage of the Cys-Gly dipeptide (PubMed:33303633).
Optimum pH is 6.5-8.5.
Optimum temperature is 30-35 degrees Celsius.
Optimum pH is 10 for the oxidation reaction, and 4-9 for the reduction reaction.
Optimum temperature is 25-28 degrees Celsius.
Optimum pH is 3.0 at 70 degrees Celsius with ABTS as substrate, and 6.0 with guaiacol and syringaldazine as substrate.
Optimum temperature is 70 degrees Celsius at pH 3.0 with ABTS as substrate. Retains 100% of its activity after 1 hour at 30 degrees Celsius at pH 9.0. Retains more than 60% of its activity after 180 minutes at 60 degrees Celsius at pH 9.0. Retains approximately 50% of its activity after 90 minutes at 70 degrees Celsius at pH 9.0.
Optimum pH is 6.5. Half-maximal activity between pH 5.0-7.5.
Optimum temperature is 90 degrees Celsius. Activity drops sharply above 90 degrees Celsius. Stable up to 100-104 degrees Celsius.
Optimum pH is 5.2.
Optimum temperature is about 50 degrees Celsius.
Optimum pH is 7 (at 30 degrees Celsius).
Broad optimum pH around 8.0.
Optimum pH is 4.0-5.5. Activity decreases above pH 5.5 and reaches negligible levels at neutral pH and above.
Optimum pH is 9. The activity remained constant to pH 10.0. Below pH 9, the activity gradually declined, and the enzyme was inactive at pH 6 or below.
Optimum pH is 8 and the values at pH 7.5 and 7.0 result in 60% and 80% decreases in activity, respectively. No pH values above pH 8.0.
Optimum temperature is 37 degrees Celsius with about 10% decrease in activity at 30 and 45 degrees Celsius.
Melting point at 81 degrees Celsius.
Thermostable. Retains almost full activity after 10 min at 65 degrees Celsius.
Optimum pH is 7.0. is active between pH 4.0 and 9.0.
Optimum temperature is 70 degrees Celsius. Ndx1 is stable up to 95 degrees Celsius at pH 7.5.
Optimum pH is 4.0-5.0. Activity is very high over a broad pH range from 4.0 to 9.0. Exhibits more than 70% activity at pH 3.5 and 9.5.
Optimum temperature is about 60 degrees Celsius. Is fully stable at this temperature.
Optimum pH is 7.5-8.5. Inactive below pH 4.6.
Highly thermostable. Is half-inactivated at 65 degrees Celsius.
Optimum pH is 4.6.
Optimum pH is 6.3 (Ref.1, PubMed:25043129). High activity and stability in the pH range from 5.0 to 8.0 (PubMed:24348183).
Optimum temperature is 45-50 degrees Celsius (Ref.1, PubMed:24348183, PubMed:25043129). Stable up to 60 degrees Celsius (PubMed:24348183, PubMed:25043129).
Optimum temperature is 85 degrees Celsius. Highly thermostable, with no loss of activity after 60 min at 100 degrees Celsius. Enzyme activity is observed at temperatures as high as 93 degrees Celsius.
Optimum pH is 8.8. The activities remain high at more alkaline pH but decrease sharply at the acidic side of the optimal pH.
Optimum temperature is 45-55 degrees Celsius.
Optimum pH is 11.0.
Optimum temperature is 100 degrees Celsius.
Optimum pH for hemagglutinating activity is 5-7 with activity decreasing quickly at higher or lower pH.
Hemagglutinating activity stable up to 50 degrees Celsius but diminishes with higher temperatures and is absent at 60 degrees Celsius.
Optimum pH is around 7.3.
Optimum temperature is 40 degrees Celsius. Retains the bulk of its activity after incubation for 1 hour at 90 degrees Celsius.
Optimum pH is 6.5 to 6.7.
Optimum pH is 4.5 to 5.0 in the presence of micromolar levels of Ca(2+) and PIP2. Optimum pH is 5.5 to 6.5 in the presence of millimolar levels of Ca(2+).
Optimum pH is 10.5 for oxidative deamination, and 9.5 for reductive amination. Retains more than 80% of its activity after incubation for 30 minutes at pH 4.0-10.5.
Optimum temperature is 80 degrees Celsius for oxidative deamination. Retains full activity after incubation for 10 minutes at temperatures up to 105 degrees Celsius.
Optimum temperature is 30 degrees Celsius for activity with ONPG as substrate. Loses half of its activity at 30 degrees Celsius within 1 hour. Inactivated at 40 degrees Celsius in 10 minutes. In the absence of glycerol, the enzyme is extremely unstable even at low-temperature incubation, with an 85% loss of activity at 20 degrees Celsius after 1 hour.
Optimum pH is 4. Unstable above pH 8.
Optimum pH is 5-7.
Optimum pH is 8.5 with (R)-reticuline as substrate. Optimum pH is 7.0-9.0 with (S)-reticuline as substrate.
Optimum pH is 8.5. About 40% and 50% of the maximum activity are observed at pH 7.0 and 10.0, respectively.
Optimum temperature is 70 degrees Celsius. No activity is detected at temperatures below 45 degrees Celsius. At 85 degrees Celsius, the activity is 85% of the maximum activity.
Optimum pH is 6.0. Active in a broad pH range of 4.5-10.0. Low activity is found at pH 10.0 and no significant activity is found at pH 4.0.
Thermostable. Retains full activity after 20 minutes at 90 degrees Celsius and 75 % of its initial activity after 20 minutes at 100 degrees Celsius.
Optimally active at pH 6.5-8.
Optimum temperature is 65 degrees Celsius. However, half-life of the protein at those temperatures is low (approximately 2 minutes).
Optimum temperature is under 41 degrees Celsius.
Optimum pH is 6.8 with carboxymethyl cellulose (CmC) as substrate.
Optimum temperature is 60 degrees Celsius with carboxymethyl cellulose (CmC) as substrate.
Optimum pH is 3.5-6.5.
Optimum pH is 6-9.5.
Optimum pH is 7. Retains less than 20% of activity at pH 9 (PubMed:23410922).
Optimum temperature is 37 degrees Celsius. Displays a half life of 28.2 hours and 12.8 hours at 37 and 40 degrees Celsius, respectively, but at 50 degrees Celsius the half life time drops to 6 minutes (PubMed:23410922).
Optimum pH is between 8 and 9.5.
Optimum pH is 4.0-6.0.
Thermostable. Highly active at 95 degrees Celsius.
Optimum pH is 4.5-8.0.
Optimum pH is 7.5-9.5 with 4-guanidinobutyrate as substrate, and 9.5-10 with D-arginine or L-arginine as substrate.
Optimum temperature is 50 degrees Celsius. Stable at 55 degrees Celsius for 30 min.
Optimum pH is 7 (PubMed:25004978). Active between pH 6-8.5 (PubMed:25004978).
Optimum temperature is 50 degrees Celsius (PubMed:25004978). Active between 10-60 degrees Celsius (PubMed:25004978).
Optimum pH is 3.5-4.5.
Optimum temperature is 55 degrees Celsius. Activity drastically decreases at 60 degrees Celsius.
Optimum temperature is 40 degrees Celsius. Inactive at 55 degrees Celsius.
Optimum pH is 7.0 for the reduction of 11-DOC, and 9.5 for the oxidation of D-gluconate.
Optimum temperature is 37 degrees Celsius for the reduction of 11-DOC.
Optimum pH is 8.5 for TEA transport (PubMed:10216142). Optimum pH is 8.4 for MPP(+) transport (PubMed:12176030).
Optimum pH is 5.0-7.0.
Has the lowest denaturation temperature of 56.5 degrees Celsius at pH 7. Binding to DPC micelles does not affect the thermal stability in alpkaline pH. Has the lowest denaturation temperature of 71.7 degrees Celsius at pH 2. Binding to DPC micelles strongly increases the thermal stability in acidic pH.
Active at temperatures close to 0 degree Celsius.
Stable transporter activity from pH 6 to pH 8.
Optimum pH is 6-7. Active fom pH 6 to pH 8.5.
Optimum temperature is 30-40 degrees Celsius.
Optimum pH is 6.0 at 65 degrees Celsius. Retains half maximum activity at pH 7.5 at 65 degrees Celsius.
Optimum pH is 7.5. Retains 36% of maximum activity at pH 5.0 and 10% of maximum activity at pH 9.5.
Optimum temperature is 60-70 degrees Celsius. Stable for 10 minutes at 50 degrees Celsius or lower, 64% of activity remains following 10 minutes incubation at 55 degrees Celsius and 14% of activity remains following 10 minutes incubation at 60 degrees Celsius.
Optimum pH is 6.6-8.6 for chlorophyll a hydrolysis, and 6.6-7.6 for chlorophyll b hydrolysis.
Thermostable between 20-43 degrees Celsius.
Thermostable up to 77 degrees Celsius. Complete thermal denaturation at 90 degrees Celsius. Thermal stability decreases in the presence of a reducing agent, dithiothreitol (DTT). The acidic and basic chains have lower thermal stabilities than the native multimeric protein.
Activity decreases below pH 5.5 and above pH 8.0.
Optimum pH is 9 for the forward reaction, and 6 for the reverse reaction.
Optimum pH is 5.5 for inulin hydrolysis.
Optimum temperature is 55 degrees Celsius for inulin hydrolysis. Is rapidly inactivated at 60 degrees Celsius. High stable at 50 and 55 degrees Celsius.
Optimum pH is 9-10.5.
Optimum temperature is 37-40 degrees Celsius. Activity decreases at 50 degrees Celsius.
Relatively stable, it loses only 25% of its total activity after 4 hours at 60 degrees Celsius. The enzyme is much more active at temperatures above 37 degrees Celsius, particularly in the reductive amination. The velocity almost doubles at temperatures between 60-65 degrees Celsius compared with 37 degrees Celsius. Above 65 degrees Celsius there is a sharp decrease in activity, due to thermal inactivation of the enzyme.
Optimum pH is 8.5 for the retroaldol activity on F6P.
Is not thermally stable at temperatures higher than 60 degrees Celsius. Is less thermostable than the isozyme FsaA.
Optimum pH is 7.8. Activity decreases sharply with increasing acidity and is null at pH 7. There is only a slight decrease toward higher pH.
Optimum pH is 7.5. Almost no activity is detected below pH 7.0 or above pH 8.5.
Long-term stability up to 80 degrees Celsius. Fructose 1,6-bisphosphate (FBP) increases the thermal stability at 90 degrees Celsius (pH 6.0).
Optimum pH is 7.2-7.8 for isozyme alpha, and 7.2-7.5 for isozymes beta and gamma.
Thermostability of the isozymes, calculated as the temperature causing half maximal stability, is 42-43 degrees Celsius for isozymes alpha and beta and 45 degrees Celsius for isozyme gamma.
Optimum pH is 7.5. Active over a wide pH range.
Optimum temperature is about 90 degrees Celsius.
Optimum temperature is 32 degrees Celsius.
Optimum pH is 5.6. Active from pH 4.0 to 8.0.
Optimum pH is 7.0 with DMS as substrate.
Optimum temperature is 25 degrees Celsius with DMS as substrate.
Optimum pH is 6.0. Stable over a wide pH range of 3.0 to 7.0.
Optimum temperature is 50 degrees Celsius. Stable up to 55 degrees Celsius but inactivated rapidly above 55 degrees Celsius.
Optimum pH is 9.0-10.0.
Optimum pH is 4.0 (PubMed:29574614, PubMed:29642287). More than half of the maximal activity is observed over a range between pH 3.3 and 4.0 (PubMed:29574614).
Optimum pH is about 5.0 with n-propyl gallate as substrate, and another smaller pH optimum is observed spanning pH 9.5-10.5. Optimum pH is 10.5 with dopamine as substrate.
Active from 20 to 43 degrees Celsius.
Optimum pH is about 7.4.
Optimum pH is 7.9 at 25 degrees Celsius.
No decrease in activity observed after incubating at pH 2.5, pH 7.4 and at pH 11.0 for 1 hour to overnight (PubMed:16839309, PubMed:23075397).
Thermostable (PubMed:16839309, PubMed:23075397). No decrease in activity was observed after heating for 1 hour at up to 95 degrees Celsius (PubMed:16839309, PubMed:23075397).
Optimum pH is 9 with acetylputrescine, N(1)-acetylspermidine, and N(8)-acetylspermidine as substrates, and is 8 with acetylcadaverine as substrate.
Optimum pH is 7 with linoleate as substrate, and 8.5-9.0 with oleate as substrate.
Optimum temperature is 25-30 degrees Celsius. Active up to 45 degrees Celsius, less active after 50 degrees Celsius and completely inactive at 70 degrees Celsius.
Optimum temperature is 10 degrees Celsius.
Optimum temperature is 30 degrees Celsius. Active over a temperature range from 20 to 70 degrees Celsius. Retains 100% of its initial activity following incubation at 40 degrees Celsius, and 82% of its activity following incubation at 50 degrees Celsius. The activity decreases significantly to 30% of the initial activity following incubation at 60 degrees Celsius.
Optimum pH is 9.7-9.8.
Stable up to 80 degrees Celsius.
Optimum pH is 7.6 with 6-hydroxynicotinic acid as substrate. Stable between pH 6.0 and 9.0.
Optimum temperature is 35 degrees Celsius with 6-hydroxynicotinic acid as substrate.
Hemagglutinating activity is stable between 30 and 60 degrees Celsius.
Optimum pH is 7.5 (PubMed:25056927). Active at pH between 6.0 and 9.0 (PubMed:25056927).
Optimum pH is about 7.5.
Optimum pH for hemagglutinating activity is 8. Activity drops with more acidic and basic pH and is lost at pH 5 and pH 8, respectively.
Hemagglutinating activity is stable after incubation at 70 degrees Celsius for 1 hour but lost at higher temperatures.
Optimum pH is 5.6. Stable from pH 4.4 to 7.6.
Optimum temperature is 45 degrees Celsius. Thermostable for 40 min from 4 to 45 degrees Celsius.
Optimum pH is under 6.0.
Optimum pH is between 5.5 and 6.
Thermostable. Displays an activity half-life of 1 day at 52 degrees Celsius.
Optimum pH is 7.4. No activity between pH 4.0 and pH 6.0, 80% activity at pH 8.0 and 20% activity at pH 9.0.
Activity stable between 4 and 40 degrees Celsius, declines at higher temperatures and is lost at 90 degrees Celsius.
Optimum temperature is 50 degrees Celsius. The activity of D-psicose 3-epimerase is very stable below 45 degrees Celsius but decreases above 50 degrees Celsius with increasing reaction time.
Optimum temperature is 60 to 65 degrees Celsius.
Optimum pH is 4.1.
Optimum pH is about 7.8. Is completely inactive at pH below 4.0 and above 10.0.
Optimum pH is 7.8-8.0.
Optimum pH is 5.0-6.0.
Optimum temperature is 55 degrees Celsius (PubMed:29691540). Active up to 70 degrees Celsius (PubMed:29691540).
Optimum pH is 7. More than 85% of the activity is retained in the pH range 4.5-6.0. Activity decreases sharply at pH 7.5.
Optimum pH is 9.0. Is active over a pH range from 7 to 9.
Retains its full activity until 30 degrees Celsius. A significant loss in the activity (65% loss) is noted at 40 degrees Celsius. Complete inactivation of the enzyme occurs at temperatures greater than 50 degrees Celsius.
Optimum pH is 9.0. At pH 9.5, retains more 60% of its maximum activity. Inactive below pH 6.
Thermal denaturation midpoint (Tm) is 71 degrees Celsius at pH 6 and is lowered around 58 degrees Celsius at pH 8.5 and 10.
Optimum pH is 8.0 with pNP-beta-D-Gal as substrate.
Optimum temperature is 40 degrees Celsius with pNP-beta-D-Gal as substrate.
Optimum temperature is 32-37 degrees Celsius.
Optimum pH is 9.0 for the linalool dehydratase activity.
Optimum temperature is 35 degrees Celsius for the linalool dehydratase activity.
Optimum pH is around 8.2 and 8.0 for glycine and sarcosine, respectively.
Optimum pH is 7.5. Stable from pH 5.0 to 8.0.
Fully active at 0 or 30 degrees Celsius, inactive at 55 degrees Celsius.
Optimum pH is 7.5 (PubMed:3308850). Retains over 65% of its maximum activity between pH 6.6 and 8.5 (PubMed:3308850).
Optimum pH is 9.0 with CGGAIISDFIPPPR peptide as substrate.
Optimum pH is 7.5. Active from pH 5 to 9.
Optimum pH is 4.9.
Optimum pH is 6.0-7.5.
Optimum pH is 7 for SGZ and bilirubin oxidation (PubMed:11514528, PubMed:11884407, PubMed:16391148). Optimum pH is about 4 for ditaurobilirubin oxidation (PubMed:16391148). Optimum pH is below 3.0 for ABTS oxidation (PubMed:11884407). Optimum pH is 8.0 for UB oxidation, whereas optimum pH for DB oxidation is 5.5 (PubMed:33618226).
Highly thermostable (PubMed:11884407, PubMed:16391148). Optimum temperature is 45 degrees Celsius for SGZ oxidation (PubMed:11514528). Optimum temperature is 75 degrees Celsius with ABTS as substrate (PubMed:11884407).
Optimum temperature is 75 degrees Celsius. Activity declines drastically at temperatures from 75 to 80 degrees Celsius.
Optimum temperature is 70 degrees Celsius. Stable at high temperature. 80% of the activity is retained after treatment at 90 degrees Celsius for 60 min.
Optimum pH is 10.5 with 1,2-hexanediol as substrate. Optimum pH is 4.0 with acetoin as substrate.
Optimum pH is 5.4-8.9.
Optimum pH is 7.3. Is stable from pH 5.5 to 8.5.
Optimum temperature is 33 degrees Celsius. Loses 50% and 100% of activity after 10 minutes of heating at 45 degrees Celsius and 50 degrees Celsius, respectively.
Optimum pH is 7.5 in the presence of magnesium and cobalt ions (PubMed:7937767, PubMed:19695264). Optimum pH is 7 in the presence of manganese ions (PubMed:7937767).
Optimum temperature is 15-37 degrees Celsius.
Optimum pH is 7.2-8.8 for the propionyl-CoA carboxylase activity measured for the holoenzyme.
Optimum temperature is 32 degrees Celsius. Active between 17 and 32 degrees Celsius.
Optimum temperature is 57 degrees Celsius.
Optimum pH is about 10.5 for the oxidative deamination of meso-diaminopimelate and 7.5 for the reductive amination of L-2-amino-6-oxopimelate. The rate of the amination reaction declines markedly above pH 9.0.
Optimum pH is 8.5 for leucine aminopeptidase activity.
Optimum pH is 8.5-9.0. Inactive at pH 7.0 or 7.5.
Activity increases gradually over the pH range 6.5-8.5.
Optimum pH is 5.5 and 7.5.
Optimum pH is 3.7. More than half of the maximal activity is observed over a range between pH 3.5 and 4.5.
Optimum pH is around 6. Stable from pH 2 to 10.
Thermostable. Active from 25 to 100 degrees Celsius. Retains 80% of its maximal activity after heating for 2 hours at 100 degrees and retains around 30% of its maximal activity after heating for 4 hours at 100 degrees.
Optimum temperature is 45-52 degrees Celsius.
Stable at pH 5.0.
Thermostable, retains activity after heating at 60, 80 and 100 degrees Celsius for 1 hour. Long-term storage at 4 degrees Celsius or -20 degrees Celsius does not affect activity.
Optimum pH is 9.3.
Optimum temperature is 37 degrees Celsius. Full activity is retained after incubating at up to 45 degrees Celsius for 10 minutes, while only 46% is left after incubating at 50 degrees Celsius. The protein is completely denatured after incubating at higher temperatures.
Active between 20.0-37.0 degrees Celsius. Activity increases above 25 degrees Celsius.
Optimum pH is about 5.
Optimum pH is 6.5-10.
Optimum temperature is 80 degrees Celsius. Activity decreases at 90-100 degrees Celsius.
Optimum pH is 7-9. The activity is relatively constant between pH 6.5 and 9 but declines sharply below pH 6.
Optimum pH is 7.0 to 8.0.
Optimum temperature is 81 degrees Celsius. Thermostable.
Optimum pH is 8.5 for racemization and 9.5 for dehydration.
Optimum pH is 7.0 with maltohexaose as acceptor substrate.
Optimum temperature is 30 degrees Celsius with maltohexaose as acceptor substrate.
Optimum pH is 5.0. Slowly inactivated above pH 8.0 and below pH 3.
Optimum temperature is 45 degrees Celsius with p-nitrophenyl-alpha-D-galactopyranoside as substrate.
Optimum pH is 8.5 for TEA uptake.
Optimum temperature is 66 degrees Celsius.
Optimum pH is 7.5-8.8.
Optimum pH is 7.8 to 7.9.
Optimum pH is 6.0 for the catalase reaction.
Optimum pH is 5.8-6.8.
Optimum pH is 7.0 to 7.4.
Optimum pH is 4.5 (at 37 degrees Celsius).
Optimum pH is around 7.4 and 7.9 for glycine and sarcosine, respectively.
Optimum pH is 4.4 with 2,6-dimethoxyphenol as substrate, and less than 2.7 with ABTS as substrate.
Optimum temperature is 55 degrees Celsius. Half-life at 70 degrees Celsius is 30 minutes.
Optimum pH is 7.0-7.7.
Optimum pH is 6.6-6.9.
Thermostable. Loses 5% of activity at 100 degrees Celsius after 1 hour incubation.
At pH below 7.5, MtbI produces isochorismate and at pH 8 it produces salicylate.
Optimum pH is 9.0. Activity is stable from pH 7 to 11.
Optimum temperature is 40 degrees Celsius. Activity is stable from 30 to 60 degrees Celsius and.
Optimum temperature is not reached at 100 degrees Celsius.
Optimum pH is around 6.8 for Gsp synthetase activity.
Optimum pH is about 9.8.
Optimum pH is about 8.5. Active from pH 6 to 12.
Displays a broad temperature optimum and is active in the range from 20 to 75 degrees Celsius. Although no significant loss of activity is detected after 600 hours of incubation at 45 degrees Celsius (at pH 8.0), the respective half-lives of the enzyme are 200 hours at 55 degrees Celsius, 30 hours at 65 degrees Celsius, and 16 hours at 75 degrees Celsius.
Optimum pH is below pH 6.0 with (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoic acid as substrate.
Optimum pH is 6.4. Active over a broad pH range.
Optimum pH is 6.7 for O-acetylserine sulfhydrylase A activity and 8.1-8.8 for cystathionine beta-synthase activity.
Optimum temperature is above 90 degrees Celsius for S-sulfo-L-cysteine synthase activity, 70-80 degrees Celsius for O-acetylserine sulfhydrylase A activity, and 80 degrees Celsius for both cystathionine beta-synthase and L-serine sulfhdrylase activities.
Optimum temperature is 25-35 degrees Celsius.
Optimum pH is between 7.5 and 9.
Optimum pH is 8.0-8.5 for CO(2) hydration, and pH 6.0 for hydrogencarbonate dehydration.
Optimum pH is 5.5. Stable between pH 5.0-6.0.
Stable up to 37 degrees Celsius.
Stable up to 57 degrees Celsius.
Optimum pH is 8 when measured with benzyl viologen. Half-maximal activities are obtained at pH 9 and pH 6.8.
Optimum temperature is 95 degrees Celsius. Is highly thermostable, retaining 85% activity after incubation for 9 hours at 90 degrees Celsius and 88% activity after 1 hour at 95 degrees Celsius.
Optimum pH is 8.0 (for L-aspartate oxidation) (PubMed:18226609). Optimum pH is about 10.0. Stable between pH 7.0 and 10.0 (PubMed:23371294).
Optimum temperature is 65 degrees Celsius. Thermostable up to 80 degrees Celsius.
Optimum pH is 8.0-11.0.
Optimim pH is 4.3. Active from pH 3 to 7.5.
Optimum pH is 9 with alanine and glyoxylate as substrates.
Optimum temperature is between 30 and 80 degrees Celsius with alanine and glyoxylate as substrates.
Optimum temperature is 70-75 degrees Celsius, enzyme is fully active after 16 hours at 90 degrees Celsius.
Optimum pH is 7.5. No activity below pH 5.5 or above pH 10.5.
Optimum pH is 10.3 for the oxidative deamination of L-leucine.
Thermostable. Retains full activity on heating at 65 degrees Celsius for 1 hour, but loses the activity completely on heating at 70 degrees Celsius for 1 hour.
After incubation at 45 degrees Celsius for 30 minutes RpiB retains 65% of its original activities. At 60 degrees Celsius RpiB is rapidly inactivated.
The optimal activity is at pH 8.8 for manganese-containing form and pH 8.5 for cobalt-containing form. The oligomer is thermostable and retains full initial activity at 40-60 degrees Celsius.
Optimum pH is 7.5 for AHBA synthase activity. Retains its activity over a broad range of pH. Over 84% of the maximum activity of AHBA synthase is maintained over a pH range from 7.0 to 9.0.
Optimum temperature is 33 degrees Celsius for AHBA synthase activity. Retains its activity over a broad range of temperature. Over 84% of the maximum activity of AHBA synthase is maintained over a temperature range from 28 to 50 degrees Celsius.
Optimum pH is 6.5. Stable from pH 7.0 to 9.0.
Optimum temperature is 37 degrees Celsius. It lost all its activities at 55 degrees Celsius.
Optimum temperature is 23 degrees Celsius. Vey low activity at 37 degrees Celsius.
Most efficient at 45-50 degrees Celsius.
Optimum pH is 7.5. This optimal pH, slightly higher than normal physiological conditions, may be necessary to ensure that the proper ionic nature of the zwitterionic anthracycline substrates is maintained for optimal recognition.
Optimum pH is between 8.5 and 10.5.
Optimum pH is 7.9 in Tris buffer and 7.6 in HEPES buffer.
Optimum temperature is 36 degrees Celsius.
Optimum pH for T3 binding is 6.0-6.5. Increase in pH causes T3 binding to drop, does not bind T3 above pH 9.0 or below pH 5.0.
Optimum temperature is 39 degrees Celsius. Is relatively thermostable since preincubation at 60 degrees Celsius for 5 minutes results in only partial inactivation of the enzyme (53% of control).
Optimum pH is 9.5-12.
Optimum pH is between pH 6.0 and 7.0 or the hydrolysis of choloyl-CoA. No activity is detected below pH 4.0 or above pH 9.0.
Optimum temperature is around 99 degrees Celsius. Extremely thermostable.
Optimum pH is 6.4-6.8.
Optimum temperature is 45 degrees Celsius. Activity sharply decreases above this temperature.
Optimum temperature is 30 degrees Celsius. Inactivated above 40 degrees Celsius.
Stable at pH 4.0-8.0.
Stable at 30-44 degrees Celsius. Forms aggregates above 44 degrees Celsius.
Optimum temperature is 90 degrees Celsius. Thermostable.
Optimum temperature is 72-76 degrees Celsius for RNase P reconstituted with this protein as well as protein subunits Rnp1-4.
Optimum pH is between 5-6. Activity decreases sharply when the pH is lowered from 5 to 4.
Stable from pH 4.0 to 10.
Stable from 4 to 40 degrees Celsius.
Active from pH 4 to 9.
Active from 60 to 95 degrees Celsius.
Optimum pH is 8.1-8.6.
Optimum pH is between 6.5 and 7.
In the range between 25 and 55 degrees Celsius, the activity increases with rising temperature. Above 55 degrees Celsius, it decreases and becomes almost zero at 65 degrees Celsius.
Optimum pH is 9.0. Active between pH 7.0 and 10.5.
Optimum pH is 5.5-7.0.
Optimum pH is 8.0-8.2 for the propionyl-CoA carboxylase activity as measured with the holoenzyme.
Optimum pH is 6.0. Stable from pH 5.0 to pH 6.5.
Optimum pH is 7.5-8. Activity is less than 10% at pH 6.0.
Optimum temperature is 30-33 degrees Celsius.
Optimum pH is 5.3 at 25 degrees Celsius.
Optimum temperature is 98 degrees Celsius. Poorly active at 25 degrees Celsius. Thermostable up to 100 degrees Celsius.
Optimum temperature is 20 degrees Celsius for complex formation activity of the N-terminus and 33.5 degrees Celsius for nuclear localization of the protein in vitro.
Optimum pH is 7.5. Active from pH 6.5 to 9.
Optimum temperature is 90 degrees Celsius. Active from 60 to 105 degrees Celsius. Highly thermostable.
Highly thermostable: is fully active after 60 minutes at 100 degrees Celsius and loses less than 10 per cent of its activity after 100 minutes at the same temperature.
Inactive at pH 6.5 or above.
Extremely thermostable.
Optimum temperature is 70 degrees Celsius. Loses activity at 85 degrees Celsius.
Optimum pH is 8 for OATase activity and 7.5-8 for AGSase activity.
Optimum temperature is 90 degrees Celsius (OATase activity).
Optimum pH is 8.0. Active from pH 6.8 to 9.5.
Optimum temperature is higher than 98 degrees Celsius. Thermostable for 2 hours up to 100 degrees Celsius.
Optimum pH is 5.0-9.0.
Optimum pH is 4.0. Stable between pH 3.0 and 7.0.
Optimum temperature is 40 degrees Celsius. Thermostable up to 45 degrees Celsius.
Displays high thermal stability. The half-denaturation temperature (Tm) is about 74 degrees Celsius.
Stable under extremes of pH.
Stable under extremes of temperature.
Optimum pH is 8.5 with cinnamic acid as a substrate.
Optimum pH is 8.25.
Optimum temperature is 25 degrees Celsius. Retains 20 to 40% of the maximum activity at 0 to 5 degrees Celsius.
Optimum pH is 7.0 for PGM activity.
Optimum temperature is 90 degrees Celsius for PGM activity.
Optimum pH is 5.6.
Thermostable. Fully active at 80 degrees Celsius.
Optimum temperature is 35-40 degrees Celsius.
Optimum pH is 6.7. At pH 6.7, glyceraldehyde is the predominant substrate, however at pH 7.5 the dehydrogenase exhibits activity preferentially towards the aliphatic aldehydes such as formaldehyde, acetaldehyde and propionaldehyde.
Optimum pH is 5.0. Stable from pH 2.5 to 7.0.
Optimum temperature is approximately 40 degrees Celsius.
Optimum pH is approximately 5.5. Loses activity rapidly at pH 4-5.
Optimum pH is 5.5-6.5 for Fe(2+) uptake.
Optimum pH is around 5.0-6.0. Retains more than 80% of activity at pH range between 4.5 and 6.5. Retains 10% of activity at pH 4.0.
Optimum temperature is 90 degrees Celsius. Retains 90% of activity even at 95 degrees Celsius. Retains 80% of activity during a 12-hour period of incubation at 70 degrees Celsius. Half-lives at 80 and 90 degrees Celsius are 23 and 4.7 hours, respectively.
Optimum pH is 7-7.5 with salicylic acid as substrate (PubMed:14630969). Optimum pH is 6.5-7.5 with benzoic acid as substrate (PubMed:14630969).
Optimum pH is 7.5-8.4 (PubMed:4945109, PubMed:4554913, PubMed:12846577). Stable between pH 6.1 and 12, however, below pH 6.0, thioesterase rapidly loses activity (PubMed:4554913).
Protease is stable up to 50 degrees Celsius.
Optimum pH is about 8.6.
Optimum temperature is 50 degrees Celsius at pH 8.6. Relatively thermostable. Activity begins to decrease significantly when E4PDH is incubated at 50 degrees Celsius for 5 min.
Optimum pH is 6.8. Active from pH 5.5 to pH 7.5. Stable from pH 3.5 to pH 10.5.
Optimum pH is 7.5 with alpha-naphthyl acetate as substrate.
Optimum temperature is between 35 and 45 degrees Celsius, with maximal activity near 40 degrees Celsius with alpha-naphthyl acetate as substrate.
Optimum pH is 7.5 for the truncated enzyme.
Optimum pH is 8.9.
Optimum temperature is 90 degrees Celsius. Extremely thermostable.
Optimum pH is 5.5-6.5.
Optimum pH is 6.0-8.5 (PubMed:8940025). Optimum pH for geranylgeranyl-P-P phosphatase reaction is 5.0-5.5 (PubMed:10329682).
Optimum temperature is 26 degrees Celsius for hemagglutination activity.
Optimum pH is between 5.5 and 6.5 with GPG and MPG as substrates.
Optimum temperature is 50 degrees Celsius with GPG and 60 degrees Celsius with MPG. With GPG as substrate, the activity becomes undetectable below 20 degrees Celsius and above 70 degrees Celsius, and with MPG as substrate, the activity is almost undetectable at 30 degrees Celsius. At 50 degrees Celsius, divalent cations are absolutely required for GpgP activity with GPG and MPG as the substrates.
Optimum pH is 5.4 for phosphatase activity using pNPP as the substrate.
Stable at wide pH range from 4 to 9.
Temperature-sensitive. Stable at 15 degrees Celsius. Starts to aggregate at 37 degrees Celsius.
Optimum temperature is 57 degrees Celsius. Incubation that exceeds 20 minutes above 50 degrees Celsius leads to enzyme inactivation.
Optimum pH is 6.0. Stable at pH 4.5-5.0, and a loss of less than 10% of the initial activity is observed after 1 hour of incubation at these pH.
Optimum temperature is 70 degrees Celsius. Stable at temperatures up to 70 degrees Celsius, with a loss of less than 10% of the initial activity observed after 1 hour of incubation at these temperatures. The enzyme shows a half-life of 1 hour at 80 degrees Celsius.
Optimum pH is 7.5-8.5. Little activity below pH 6.5.
Optimum pH is between 7.5 and 9.5 with COMP for substrate.
Optimum pH is 7.0 for Pyr2C reduction and 10.0 for L-proline oxidation.
Optimum pH is 7.2-8.4.
Thermal stability decreased at temperatures above 40 degrees Celsius.
Optimum pH is 7.5. At pH values above 7.5, the activity sharply decreases.
Optimum activity at 40 degrees Celsius.
Optimum pH is 7.8-8.4.
Optimum pH is 4-5.
Optimum pH is 5.5 - 7.5.
Optimum pH is 5.5 with chitotriitol as substrate, and 5.3 with (GlcN)2 as substrate. Activity is lost below pH 3.5 and above pH 8.0.
Optimum temperature is 60 degrees Celsius. Stable below 50 degrees Celsius, inactive above 60 degrees Celsius.
Optimum temperature is 90 degrees Celsius. GpgS is inactive at 40 degrees Celsius, but the activity increases dramatically above 50 degrees Celsius. At 100 degrees Celsius, GpgS retains 33% of the total activity.
Optimum pH is 5. Active from pH 2.5 to 6.0.
Optimum pH is 3.
Optimum pH is 6.5 at 30 degrees Celsius.
Optimum pH is 7.2-7.8.
Optimum pH is 4.0. Retains greater than 90 percent of its original activity between pH 2.0 and 7.0 at room temperature for 3 h.
Optimum temperature 70 degrees Celsius. Remains stable up to 60 degrees Celsius for 30 min, but loses activity at 80 degrees Celsius.
Optimum pH is 7.5 for both the forward and the reverse reactions.
Optimum temperature is 35-46 degrees Celsius.
Optimum pH is 9.0 with [5-3H]dUMP as substrate, and 7.0 with Br-dUMP as substrate.
Optimum temperature is 70 degrees Celsius with [5-3H]dUMP as substrate, and 50-70 degrees Celsius with Br-dUMP as substrate. Heating the protein for 10 min at 100 degrees Celsius abolished the activity.
Fully active at acidic pHs, the antiporter is virtually turned off at neutral pH.
Optimum pH is 6.0 for both lactose and ONPG hydrolysis, but lactose hydrolysis activity is more sensitive to changes in pH than ONPG hydrolysis activity.
Optimum temperature is 37 degrees Celsius for both lactose and ONPG hydrolysis. Retains 16.5% of activity for ONPG hydrolysis and 11.2% of activity for lactose hydrolysis at 4 degrees Celsius. Stable at or below 25 degrees Celsius, but loses 50% of its activity after 1 hour at 37 degrees Celsius and is inactivated after only 40 minutes at 45 degrees Celsius. Can hydrolyze 73% of lactose in milk in 30 hours at 10 degrees Celsius.
Optimum pH is 6.0 with glucose-pentaacetate as substrate. Active from pH 5.0 to 8.0.
Optimum temperature is 40 degrees Celsius with glucose-pentaacetate as substrate. Active from 30 to 50 degrees Celsius. At temperatures of 30 degrees Celsius and lower, less than 10% of the maximum activity is left. At 50 degrees Celsius, retains 60% of its maximum activity.
Optimum pH is 4.5. Active from pH 4 to pH 5.5.
Optimum pH is 5.5 with RNA and ssDNA as substrates.
Active between pH 6-3.
Active between 50-70 degrees Celsius.
Optimum temperature is 70 degrees Celsius. Thermostable up to 90 degrees Celsius, however, only in the presence of salts.
Optimum pH is 6.5 with oleate as substrate.
Optimum temperature is 25 degrees Celsius with oleate as substrate. After 120 minutes, the enzyme activity is nearly unchanged at 25 degrees Celsius, but is reduced to only 25% at 35 degrees Celsius.
Optimum pH is 5.7.
Optimum pH is between 7.5 and 8.
Optimum pH is 7.5 at 60 degrees Celsius. At 40 degrees Celsius (temperature of the natural environment for H.mediterranei) the optimum pH is 6.5.
Optimum pH is 5.75-6.5 and 8.75-9.0.
Optimum temperature is 16-23 degrees Celsius.
Optimum pH is 9 with 3-hydroxykynurenine and pyruvate, or L-alanine and glyoxylate as substrates (at 50 degrees Celsius).
Optimum temperature is 55 degrees Celsius with 3-hydroxykynurenine and pyruvate at pH 7 (PubMed:11880382, PubMed:12220660). Optimum temperature is 60 degrees Celsius with L-alanine and glyoxylate at pH 7 (PubMed:11880382, PubMed:12220660).
Optimum pH is 5.5 with azocasein as substrate, 7 with Z-Phe-Cit-NHMec, and 9.5 with Z-Phe-Arg-NHMec and Z-Arg-Arg-NHMec as substrate.
Optimum temperature is 4 to 42 degrees Celsius.
Optimum pH is about 4.0. Activity strongly decreases above this value.
Optimum temperature is about 35 degrees Celsius. Activity strongly decreases above this value.
Optimum pH is 8.0-8.2 for the propionyl-CoA carboxylase activity.
About 60% of the activity is lost in the absence of FII after 10 minutes at 40 degrees Celsius, but only 35% in the presence of FII.
Optimum temperature is 55 degrees Celsius. Retains 65 and 46 percent residual activity after a 20 min incubation at 70 and 75 degrees Celsius, respectively.
Optimum pH is 7.2 at 30 degrees Celsius. Optimum pH is 6.8 at 60 degrees Celsius.
Optimum pH is 8-8.5. Transport activity occurs from pH 7.5 to 9.
Optimum pH is <7. Is active against E.coli ATCC25922 at pH 5 (MIC=12.5 uM), whereas is almost not active at pH 7 (MIC=70 uM).
Active up to 80 degrees Celsius. Thermostable.
Optimum temperature is 90 degrees Celsius for ATPase and DNA-binding activities.
Optimum pH is 9-10 for the oxidation of ursodeoxycholate. Optimum pH is 4-6 for the reduction of 7-oxo-LCA and dehydrocholate.
Is not very thermostable. The enzyme is completely inactivated at 50 degrees Celsius within 5 minutes and at 40 degrees Celsius within 400 minutes.
Optimum pH is between 9.1 and 9.5.
Loss of protein synthesis inhibiting activity of the rabbit reticulocyte lysate by heating at 70 degrees Celsius for 20 minutes. The inhibitory activity of the solution is almost completely lost after storage for 2 weeks at 4 degrees Celsius in the presence of 1% 2-mercaptoethanol.
The activity significantly decreases to less than 20% of the original activity after 24 hours of incubation at 4 degrees Celsius.
Optimum pH is 6.5 for trehalose phosphorolysis activity, 6.5-6.8 for trehalose synthesis activity.
Optimum temperature is 32.5 degrees Celsius for trehalose phosphorolysis activity, 37.5 degrees Celsius for trehalose synthesis activity. Stable up to 35 degrees Celsius for trehalose phosphorolysis activity, 32.5 degrees Celsius for trehalose synthesis activity.
Optimum temperature is 120 degrees Celsius. Highly thermostable.
Optimum pH is 5 and 6.7 with citrate and fructose 1,6-bisphosphate (FBP) as activator, respectively.
Optimum temperature is 40-60 degrees Celsius.
Optimum pH is 6.0 for phosphorolytic activity. Optimum pH is 5.5 for the synthetic reaction.
Optimum pH is 6-9 for the oxygenation reaction and 10.5 for the oxidation reaction.
Optimum temperature is 45 degrees Celsius for the oxygenation reaction and 65 degrees Celsius for the oxygenation reaction. Stable up to 70 degrees Celsius.
Active at pH 4 and 7.4 with ecdysone 22-phosphate (E22P), 3-epi-ecdysone 22-phosphate (E22P) or 3-epi-ecdysone 2-phosphate (E2P) as substrate.
Optimum pH is 7.0 (at 60 degrees Celsius).
Optimum pH is 8.4-9.0.
Optimal transport of histidine at pH 4.5-5.
Optimum pH is 7.5-7.8.
Active at neutral to pH 8 with casein as substrate.
Active over a very broad pH range.
Optimum pH is 7.5-8.0. Is stable from pH 6.0-11.0.
Optimum temperature is 70 degrees Celsius. Is highly stable at 55 degrees Celsius.
Optimum temperature is 42-44 degrees Celsius.
Optimum temperature is 65 degrees Celsius. Thermostable at 50 degrees Celsius in buffers at pH 7.
Optimum temperature is close to 90 degrees Celsius.
Optimum pH is around 7. Relatively stable in solution within the pH range of 5-9.5.
Highly thermostable. Inactive towards methylviologen below 55 degrees Celsius.
Optimum temperature is 65 degrees Celsius. At this temperature, the enzyme is rapidly inactivated.
Optimum pH is 7-7.5. Active from pH 6 to 9.
Optimum pH is 6.4 with tamarind seed xyloglucan as substrate. Retains 50% of its activity between pH 5.7 and 7.8.
Optimum temperature is 75 degrees Celsius. Retains 25% of its activity after incubation at 90 degrees Celsius for 30 minutes.
Is more active at more active at 25 degrees Celsius than at 37 degrees Celsius.
Optimum pH is 6.0. Unstable at pH values less than 5.0 and remains fully active over a wide alkaline pH range.
Optimum temperature is 40 degrees Celsius but displays considerable activity as low as 20 degrees Celsius. Thermostable. Retains 60% of initial activity after incubation at 50 degrees Celsius for 2 hours.
Active only at acidic pHs.
Optimum pH is 7.2-7.6.
Most active from pH 5.0 to 8.0.
Optimum temperature is 70 degrees Celsius. Active from 50 to 80 degrees Celsius, at 55 degrees Celsius it has 50% of its full activity. Thermostable, retains full activity after heating at 60 degrees Celsius for 24 hours. In the absence of substrate, its half life at 70 degrees Celsius is 50 minutes. In the presence of substrate full activity is retained after incubation at 70 degrees Celsius for 2.5 hours.
Optimum temperature is 23 degrees Celsius. No detectable activity at 37 degrees Celsius.
Optimum temperature is between 20-50 degrees Celsius.
Optimum temperature is 37 degrees Celsius. The enzyme is stable up to 50 degrees Celsius. At 55 degrees Celsius, the enzyme retains approximately half of its activity but it is completely inactivated at 60 degrees Celsius.
Optimum pH is 6.8-9.7.
Optimum temperature is 70 degrees Celsius, but the enzyme is not stable above 50 degrees Celsius.
Optimum pH is 8.5. Stable at pH 5.0 to 9.5 at 30 degrees Celsius.
Hydrolyzes lactose at pH 6.5, 6.8 and 7.2.
Hydrolyzes lactose between 30-45 degrees Celsius.
Stable to heat treatment. Retains 33% of activity when heated to 100 degrees Celsius for 10 minutes at pH 7.5.
Optimum temperature is 87-99.9 degrees Celsius, has very low activity at 37 degrees Celsius after 16 hours.
Optimum pH is 7-10. Activity on 4-nitrophenyl phosphate is observed between pH 6 and pH 11.
Heat-labile. Activity increases with temperature between 15 and 35 degrees Celsius and decreases with further heating. No activity at 65 degrees Celsius. Preincubation for 10 minutes at 50 or 60 degrees Celsius causes 50% or 100% inactivation, respectively. Heat-inactivated enzyme cannot be renaturated.
Optimum pH is between 6.9 and 8.5 for both adenosine and 2-deoxyadenosine.
Optimum pH is 4.25 for the peroxidase reaction and 6.5 for the catalase reaction.
Optimum pH is 6-6.5 with 5'-AMP as substrate, and pH 5.5-6 with 3'-AMP or pNPP as substrate.
Optimum temperature 40 degrees Celsius.
Structurally relatively unstable in pure water, but more stable in various buffers between pH 5-9. Most stable in 20 mM HEPES buffer at pH 7 with the addition of 2 mM Ca(2+).
Unfolds at 47 degrees Celsius in pure water, but structurally more stable in various buffers up to 60 degrees Celsius.
Optimum pH is 6.4-7.4.
Optimum pH is 6.0 in potassium phosphate and 7.5 in HEPES buffer.
Active at pH 7.0 and 9.0, but not at pH 5.5.
Optimum pH is between 7 and 7.4.
Optimum pH is 6.5 with AZCL galactan as substrate.
Optimum temperature is 50 degrees Celsius with AZCL galactan as substrate.
Optimum temperature is 35 degrees Celsius. Activity is rapidly lost above 40 degrees Celsius.
This enzyme is thermostable.
Optimum pH is 7.8 in 50 mM Tris-HC1 and 8.5 in 50 mM NH(4)HCO(3).
Optimum pH is 8.5 for p-nitrophenyl palmitate and 7.5 for p-nitrophenyl acetate and p-nitrophenyl butyrate.
Optimum temperature is 45 degrees Celsius with p-nitrophenyl palmitate and 30 degrees Celsius with p-nitrophenyl acetate and p-nitrophenyl butyrate.
Optimum pH is 7.0 with spermidine as substrate (PubMed:24634478). Optimum pH is 6.5 with spermine as substrate (PubMed:24634478).
Optimum temperature is 37 degrees Celsius with spermidine as substrate (PubMed:24634478). Optimum temperature is 30 degrees Celsius with spermine as substrate (PubMed:24634478).
Optimum temperature is 55 degrees Celsius. It retained half of its activity after 10 min of incubation at 55 degrees Celsius.
Optimum pH is 7.2 to 7.5 for L-malate.
Optimum temperature is 50 degrees Celsius. It is stable below 50 degrees Celsius.
Optimum pH is 6.5-7.7.
Optimum pH is 7.5-8.0. Activity decreases rapidly below pH 7.0 and above pH 9.0.
Activity increases as temperature increases from 0 to 37 degrees Celsius.
Optimum pH is 6.0. Is stable from pH 5.5 to 9.0.
Optimum temperature is 40 degrees Celsius. Is stable below 40 degrees Celsius.
Optimum temperature is 80 degrees Celsius for ATPase activity.
Optimum temperature is 37 degrees Celsius with maltohexaose as acceptor substrate.
Optimum pH is around 8.0. Stable between pH 4 and 11.
Active from 25 to 40 degrees Celsius.
Optimum temperature is 24 degrees Celsius.
The MqsR-MqsA complex is exceptionally thermostable with a Tm of 83.4 degress Celsius versus 48.1 degress Celsius for MqsR and 61.1 degress Celsius for MqsA.
Optimum pH is 8.0 for meso-2,3-butanediol oxidation (PubMed:23666479). Optimum pH is 5.0 for (3S/3R)-acetoin reduction and 8.0 for diacetyl reduction (PubMed:23666479).
Optimum temperature is 40 degrees Celsius for oxidation and reduction reactions.
Optimum pH is 8.0. Active over the pH range 6.0-11.0. Stable at pH 7.5 for 20 hours at 20 degrees Celsius.
Stable below 30 degrees Celsius for 20 minutes at pH 7.0.
Optimum temperature is 70 degrees Celsius. It stays fully active upon incubation at 50 degrees Celsius. Even after 4 hours of incubation, the enzyme retains more than 95% of its initial activity.
Rapidly loses activity at 37 degrees Celsius in the absence of DNA.
Optimum pH is 8.5. Displays over 60% of maximum activity at pH 8.0-10.
Optimum temperature is 40 degrees Celsius. Retains 60% of the maximum activity at 0 degree Celsius, suggesting it is a cold-adapted enzyme.
Optimum temperature is 50-60 degrees Celsius. Activity is 22% lower at 60 degrees Celsius and 60% lower at 100 degrees. Thermostable.
Active from pH 3.0 to 10.5.
Active up to 62 degrees Celsius.
Optimum pH is 6.0. Is stable from pH 5.5 to 8.5.
Optimum temperature is 35 degrees Celsius. Is stable below 30 degrees Celsius.
Optimum pH is 7.5-8 (at 20 degrees Celsius).
Optimum temperature is 20-30 degrees Celsius (at pH 7.6).
Optimum pH is 7.0 for bradykinin. Active between pH 6.0-11.0.
Thermostable to 55 degrees Celsius. Complete loss of activity above 70-75 degrees Celsius.
Optimum temperature is 33 degrees Celsius.
Full hemagglutinating activity after 3 hours of incubation at pH 3-9 at room temperature.
The hemagglutinating activity is stable at 50 degrees Celsius up to 40 min, but decreases to 50% after 80 min.
Optimum pH for oxidative deamination is 9.2. Optimum pH for reductive amination is 8.5.
Optimum temperature is 70 degrees Celsius. The enzyme is entirely stable from 50 to 70 degrees Celsius and 55 % activity is retained after 30 min at 90 degrees Celsius.
Optimum pH is 5.5 at 30 degrees Celsius.
Activity is stable between pH 4 and 7 but decreases at basic pH.
Activity is stable up to 60 degrees Celsius, then decreases and is lost at 100 degrees Celsius.
Optimum temperature is 42 degrees Celsius. Thermostable at temperatures at or below the optimal temperature for activity, but it is rapidly denatured at temperatures above 42 degrees Celsius. Irreversibly inactivated within 10 minutes at 55 degrees Celsius. Stable during storage at 5 degrees Celsius and loses no activity during storage for 4 months. Retains 10% of activity at 0 degrees Celsius.
Optimum pH is 5.1.
Optimum pH is 8.0 at 37 degrees Celsius. Maintains over 90% of its inhibitory activity from pH 3 to 10.
Active between 10 and 60 degrees Celsius. 84% activity retained when heated for 30 minutes at 80 degrees Celsius. Incubation at temperatures above 80 degrees Celsius rapidly decreases inhibitory activity.
Optimum pH is 2.0. Stable from pH 4 to pH 7.
Optimum pH is 5.5. Activity is above 50 percent between pH 4.5 and 6.5.
Optimum temperature is 45 degrees Celsius. Activity remains at about 60 percent and 14 percent at 60 degrees Celsius and 65 degrees Celsius respectively.
Optimum pH is 7-8 for ATPase activity. Is more active at pH 8 to 10 than at pH 5.5.
Optimum pH is 9.5-11.5 for the oxidation of either (R,R)-butane-2,3-diol or meso-butane-2,3-diol and around 6.5 for the reduction of (3R/3S)-acetoin.
Optimum temperature is at least 50 degrees Celsius for the oxidation reaction and around 45 degrees Celsius for the reduction of (3R/3S)-acetoin.
Optimum temperature is 38-40 degrees Celsius.
Optimum pH is around pH 8.6. At pH 8.0-9.6 more than 90% of maximal activity is still observed.
Optimum temperature is 40 degrees Celsius. Thermolabile above 40 degrees Celsius and essentially inactive at 60 degrees Celsius.
Optimum pH is 8.0 for agmatine uptake (PubMed:21128598). Optimum pH is 8.5. Active from pH 6 to 8.5.
Optimum pH is 7.5-8.5. Loss of methyltransferase activity at pH 5.5 and below.
Optimum pH is 7.0. Stable at pH 6.0 and about 80% of the enzyme activity remained between pH 7.0 and pH 8.0.
Not extremely thermostable, has lost significant activity after 90 minues at 65 degreees Celsius, has no activity after 4 hours.
Optimum pH is 4.75 for the peroxidase reaction and 6.25 for the catalase reaction.
Optimum pH is 6.5 at 40 degrees Celsius. Retains at least 80% of activity after incubation for 24 hours at 4 degrees Celsius between pH 4.5 and pH 9.5.
Optimum temperature is 50 degrees Celsius at pH 5.0. Stable when incubated for 30 minutes at up to 50 degrees Celsius, little activity at 75 degrees Celsius or above.
Optimum pH is 7.5. Active between pH 6.5-8.5. Unfolds at pH 5.5.
Optimum pH is 7-8. Retains 24% of maximal activity at pH 5 and 14% of activity at pH 9.
Retains 50% of the activity in elution buffer/20% glycerol for four days at 4 degrees Celsius. Retains 60% of the activity in several days storage at -20 degrees Celsius with 20% glycerol.
Optimum pH is 8.0 for methyltransferase activity.
Thermal resistance is enhanced at low protein and high salt concentrations, is pH-dependent and denaturation is irreversible.
Optimum temperature is 77 degrees Celsius.
Optimum pH is 9 for PET film hydrolysis (PubMed:26965627). Optimum pH is 9 for PET (commercial drinking bottle) hydrolysis. Optimum pH is 6.5-8.0 for BHET hydrolysis (PubMed:29603535). Optimum pH is 8.0 for the hydrolysis of pNP-esters. The enzyme is active at pH 6-10, has an optimal pH range of 7-9 and it is rapidly inactivated below pH 7.0 or above pH 9.0 (PubMed:30502092).
Optimum temperature is 40 degrees Celsius for PET film hydrolysis (PubMed:26965627). Optimum temperature is 30 degrees Celsius for PET (commercial drinking bottle) hydrolysis and BHET hydrolysis (PubMed:29603535). Optimum temperature is 35-45 degrees Celsius for the hydrolysis of pNP-esters. Remains active even at 65 degrees Celsius (about 60% of maximum activity) (PubMed:30502092).
Optimum pH is 7.4-8.5. Little activity below pH 6.5.
Optimum pH is around 8.5 for dimethylglycine.
Optimum pH is between 6.5 and 7.0.
Optimum pH is 7.0. Activity is reduced to about 70% and 60% after incubation at pH 5.0 and pH 9.0, respectively, for 1 hour.
Optimum pH is around 6. Activity decreases sharply when the pH is lowered from 5 to 4.
Optimum temperature is between 30-40 degrees Celsius.
Optimum pH is about 10.
Optimum temperature is 30 degrees Celsius. Active at 30 degrees Celsius, and rapidly inactivated with an increase of 5 degrees Celsius.
Optimum temperature is below 20 degrees Celsius.
Optimum pH is 6.5-9.0.
Optimum pH is 8. More than 50% activity is retained between pH 3 and 12. Inactive at pH 2.
Activity remains stable after 30 min at 55 degrees Celsius.
Optimum pH is 5-7.5.
Optimum pH is 6-8.4 for gelatinase activity. At pH lower than 5 inhibited gelatinase activity.
Optimum temperature is 37 degrees Celsius for gelatinase activity. Temperatures above 50 degrees Celsius inhibit gelatinase activity.
Optimum pH is 9.0 (at 80 degrees Celsius) (PubMed:8830684). Optimum pH is 7.0 (at 55 degrees Celsius) (PubMed:9119024).
Optimum pH is 5.8. Approximately 80% of activity retained after incubating the enzyme for 40 minutes in buffers ranging from pH 5.0 to pH 10.5.
Optimum temperature is 70 degrees Celsius. Retains 90% of activity when heated at 70 degrees Celsius for 30 minutes. Approximately 48% of lactose in milk is hydrolyzed following treatment with enzyme at 65 degrees Celsius over 60 minutes.
Activity unaffected from pH 1-10. Loses 34% of its activity after 2 hours at pH >12 and 65% of its activity after 4 hours at pH >12.
Retains over 90% of its activity after incubation at between 25 and 55 degrees Celsius for 1 hour.
Thermostable. Highly active at 80 degrees Celsius.
Optimum pH is 4.5 with catechol as substrate.
Optimum temperature is 45 degrees Celsius with catechol as substrate. Completely inactivated after heating at 70 degrees Celsius for 10 min.
Optimum pH is 8.0. Active from pH 7.0 to 11.0.
Optimum pH is 7.0 for Pyr2C reduction, 8.0 for Pip2C reduction, and 10.0 for L-proline and L-pipecolate oxidation.
Optimum temperature is 35 degrees Celsius. Is inactivated at a rate of about a 30% decrease in 30 minutes at 35 degrees Celsius. The enzyme keeps its full activity, however, at 30 degrees Celsius for at least 30 minutes.
Optimum temperature is 51 degrees Celsius.
Optimum pH is 9.0 or between 8.5-8.7 (with Tris-HCl buffer). Half-maximal activity is observed at pH 7.4 and 9.7.
Optimum temperature is 37 degrees Celsius for native enzyme. Displays half-maximal activity at 23 degrees Celsius and 49 degrees Celsius. Rapidly inactivated at temperatures above 45 degrees Celsius. Loses more than 35% and 30% of its activity when stored at -20 degrees Celsius for 1 month and at 4 degrees Celsius for 48 hours, respectively.
Optimum pH is 4.
Optimum pH is 9.5 for racemization and 9.0 for hydration.
Optimum temperature is 35 degrees Celsius (racemase or dehydratase activity).
Optimum temperature is 27-30 degrees Celsius.
Optimum temperature is 40-70 degrees Celsius.
Optimum temperature is 35 degrees Celsius (PubMed:24714029). Active from 10 to 50 degrees Celsius (PubMed:24714029).
Optimum pH is about 7.6-8.4. Stable from pH 4.0 to 10.0.
Optimum pH is between 7-8.
Optimum pH is 4.75 and 10.5. These two pH optima may correspond to two distinct conformational states of the enzyme.
Optimum pH is about 7.8.
Optimum pH is 7.2. Active between pH 6.0 and 9.0.
Inactive above 60 degrees Celsius.
Optimum pH is 2.
Optimum pH is 5.5 for glycyl-sarcosine transporter activity.
Optimum pH is 5.0. The activity drops sharply below pH 4.5 and above pH 5.5.
Optimum temperature is 37 degrees Celsius. Retains more than 90% of activity after 96 hours of incubation at 37 degrees Celsius and after 12 hours of incubation at 42 degrees Celsius.
Optimum temperature is above 55 degrees Celsius. Active from 5 to 75 degrees Celsius. Thermostable.
Optimum pH is 9. The enzyme is stable from 8 to 10.
Optimum temperature is around 40 degrees Celsius. The enzyme is stable below 40 degrees Celsius, but decreases with increasing temperature.
Optimum pH is around 7-8.
Optimum pH is 7-8.4 for D-ribulose 5-phosphate reductase activity.
Optimum temperature is 65 degrees Celsius for the ATPase activity.
Optimum temperature is 37 degrees Celsius for the hydrolysis of TAMe (tosyl-arginine methyl ester) substrate. Thermolabile. Activity is reduced about 50% after incubation at 50-55 degrees Celsius for 30 min. Loss of activity after incubation at >60 degrees Celsius for 30 min.
Optimum pH is above 7.0 for lipase activity.
Stable at pH greater than 6.5.
Optimum pH is 6.0-8.5.
Optimum pH is 5.9-6.0.
Optimum temperature is 80 degrees Celsius for the recombinant enzyme. Thermostable up to 85 degrees Celsius.
Optimum pH is 4-7. Optimum pH is substrate-dependent.
Optimum pH is 7.5 (PubMed:18270315). Optimum temperature is 37 degrees Celsius (PubMed:18270315).
Optimum pH is 7.4 to 8.0.
Optimum pH is 7.0. Retains more than 80% of the activity at a pH range of 6.0-7.5.
Optimum temperature for the activity is 70 degrees Celsius using ONPG as substrate. Stable up to 70 degrees Celsius (PubMed:3086288). Retains 80% of the activity at 75 degrees Celsius (PubMed:18420605). Kinetics of thermal inactivation and half-life times at 60, 65 and 70 degrees Celsius are 120, 50 and 9 hours, respectively.
Optimum pH is between 4.4 and 6.0.
Inactive below pH 6.0.
Optimum pH is dependent on ornithine concentration with an optimum at pH 8 for ornithine concentrations around KM values. Higher concentrations shift the optimum towards lower pH. At pH 7.0, the maximum velocity remains constant even when the ornithine concentration is very high (> 10 mM). At more alkaline pH, however, substrate inhibition is observed above 0.25 mM ornithine.
Optimum temperature is 35-37 degrees Celsius.
Optimum pH is 7.0 for citrate.
Optimum temperature is 23 degrees Celsius. Very low activity at 37 degrees Celsius.
Optimum pH is 8.2-8.5.
Shows a maximum of activity between pH 7.5 and 8.0 both in the oxidation reaction of cholate and in the reduction reaction of 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate.
Optimum temperature is 40 degrees Celsius. No activity is detected above 60 degrees Celsius.
Has a half-life of only 1.6 hours at 50 degrees Celsius. When TTHB247 is in complex with TTHB246, its half-life is increased by approximately 4 hours.
Optimum pH is 5.5 with pNP-beta-D-glucoside as a substrate. Retains more than 90% of its activity between pH 5.0 and 7.0. Retains 70% of its activity at pH 4.5 and 8.5.
Optimum temperature is 45 degrees Celsius with pNP-beta-D-glucoside as a substrate. Retains 60% of its activity after 10 minutes incubation at 50 degrees Celsius.
Optimum pH is 6.8-8.8.
Optimum pH is 7.75. Inactive below pH 6.5.
Optimum pH is 9 using spermidine and coumaroyl-CoA as substrates.
Optimum pH is 7.5 with bis-pNPP as substrate.
Optimum pH is 5.0 with para-nitrophenyl beta-D-glucopyranoside (pNPGlc) as substrate.
Optimum temperature is 55 degrees Celsius with para-nitrophenyl beta-D-glucopyranoside (pNPGlc) as substrate.
Optimum pH is 7.3 and 50% of activity at pH 5.6 and at pH 8.6.
Optimum temperature is 60 degrees Celsius. It does not lose activity upon incubation at 60 degrees Celsius for 2 h. The half time at 70 degrees Celsius is about 5 min.
Optimum pH is 9.0 for DNA restriction and 7.5 for methylation.
Optimum pH is 5. Retains at least 50% of its maximum activity between pH 4.5 and 8.0. Is not active at pH values below 4.5, while at least 35% of maximum activity is found in the pH range 8.5-11.0.
Optimum temperature is 45 degrees Celsius. Is only stable at 55 degrees Celsius or lower temperatures. Retains more than 77% activity after 2 hours incubation at 55 degrees Celsius and pH 7.
Optimum pH is 6.5 for elastin hydrolysis.
Highly active at low temperatures, even at 0 degree Celsius. Thermolabile.
Optimum pH is 8.5-9.2 (for a construct expressing residues 84-695).
Optimum temperature is 65 degrees Celsius (PubMed:23804759, PubMed:11850422). Active between 25 and 77 degrees Celsius (PubMed:11850422).
Optimum temperature is 85 degrees Celsius. Retains 80% activity after incubation at 85 degrees Celsius for 3 hours.
Optimum pH is 4.0. Stable in the wide pH range of 3.0 to 7.0.
Stable at temperatures of up to 45 degrees Celsius and is inactivated gradually above 45 degrees Celsius.
Optimum pH is 7.2 with GGPP as substrate and 8.7 with CPP as substrate.
Optimum temperature for DNA polymerase is 60 degrees Celsius, functional between 60 and 80 degrees Celsius. Exonuclease activity is higher at 70 than 50 degrees Celsius.
Optimum pH is 5.9 in the absence of effectors. Optimum pH is 7.1 in the presence of tryptophan. Optimum pH is 5.4 in the presence of tyrosine. Tryptophan broadens the pH range of detectable catalytic activity.
Optimum pH is 7.5 for LKR activity of isoform Long, and 9 for SDH activity of both isoforms Long and Short.
Optimum pH is between 6.7 and 7.1. The enzyme is stable from pH 5.0 to 9.0. It completely losing activity at pH values lower than 4.5 and retaining some activity in the pH range 9-12.
Optimum pH is 7.0 with DV-Chlidea or DV-Pchlidea as substrate, optimum pH is 6.5 with DV-MPE as substrate.
Optimum temperature is 25 degrees Celsius with DV-Chlidea as substrate, Optimum temperature is 20 degrees Celsius with DV-Pchlidea or DV-MPE as substrate.
Optimum pH is 6-7.5.
Optimum pH is 7.5 (PubMed:16255717). Optimum pH is 9 (PubMed:15708357).
Optimum temperature is 30-60 degrees Celsius.
Optimum pH is 8. Activity decreases rapidly at higher or lower pH.
Optimum temperature for caseinolytic activity is 40 degrees Celsius. Activity decreases rapidly at higher or lower temperatures. No activity below 10 degrees Celsius or above 70 degrees Celsius.
Optimum pH is 8.5 for ergothioneine uptake and transport efficiency decreased at more acidic pHs.
Activity is stable from pH 3.5 and 10.5.
Thermostable. Activity is stable after incubation at 100 degrees Celsius for 30 min.
Optimum temperature is 37 degrees Celsius. Retains 84% of activity when incubated at 4 degrees Celsius for 3 days.
Optimum pH is 7.5 in the presence of 5 mM ornithine. The curve shifts toward a more alkaline region with a decrease in ornithine concentration.
Optimum pH is 7-9 with activity decreasing quickly at higher or lower pH.
Hemagglutinating activity stable up to incubation at 80 degrees Celsius for 1 hour but diminishes with higher temperatures and is absent at 100 degrees Celsius.
Optimum pH is about 6.5.
Optimum temperature is between 35 and 40 degrees Celsius.
Optimum pH is 7.0-7.5 with AAFP as substrate.
Optimum pH is about 5.1 (PubMed:16488399). Activity decreases with increasing pH values (PubMed:25837439).
Optimum temperature is between 45-55 degrees Celsius (PubMed:16488399, PubMed:25837439). 50% activity at 35 and 60 degrees Celsius. No activity below 20 or above 70 degrees Celsius (PubMed:25837439).
Optimum pH is 7.5-8.0. Active from pH 6 to 10. Stable from pH 5 to 11.
Optimum temperature is 50 degrees Celsius. Active from 30 to 65 degrees Celsius. Thermostable for 30 minutes up to 55 degrees Celsius.
Retains about 50% of its initial activity when heated at 85 degrees Celsius for 5 min at pH 7.2.
Optimum pH is 7.7 in phosphate buffer.
Relatively stable at high temperatures. Retains 50% of its activity after incubation at 70 degrees Celsius for 15 minutes.
Inactivated at temperatures greater than 40 degrees Celsius.
Optimum pH is 7.0 in the presence of Zn(2+). The maximal velocities are independent of pH in the alkaline region but decrease below pH 7.0.
Optimum pH is 7.0 with (Glc-NAc)n (n=3-6) as substrate and 5.8 with (GlcNAc)2 as substrate.
Optimum pH is 4.0 to 5.0.
Optimum temperature is 65 degrees Celsius. Stable up to 55 degrees Celsius.
Optimum pH is 8 with RNA, ssDNA and dsDNA as substrates.
Optimum pH is acidic with no activity detected at a pH higher that 7.0.
Optimum pH is 8.5 (PubMed:2007545). The enzyme is stable between pH 8 and 9 (PubMed:2007545).
Optimum pH is 5.4 with 1-nitrocyclohexene as substrate.
Optimum pH is 8.5 for peroxidase activity.
Optimum temperature is about 75 degrees Celsius. Activity is 10-fold greater at 75 degrees Celsius than that measured at 25 degrees Celsius. Thermostable up to 85 degrees Celsius. Thermal denaturation midpoint (Tm) is 91 degrees Celsius.
Optimum pH is 10 for the forward reaction, and 6.5-7 for the reverse reaction.
Optimum pH is about 5.5 at 50 degrees Celsius and shows an apparent shift towards higher pH values at higher temperatures.
There is nearly no change in activity from pH 5 to 10.
Optimum pH is 7.5-7.9.
Optimum pH is 7.4. Active from pH 5 to 10.
Optimum temperature is 125 degrees Celsius. Thermostable up to 137 degrees Celsius.
Optimum pH is 9.0. Active from pH 5 to 11.
Optimum temperature is 60 degrees Celsius. Active from 25 to 70 degrees Celsius.
Optimum pH is 8.4 for sulfur reductase activity at 80 degrees Celsius.
Optimum temperature is 80 degrees Celsius for maximal sulfur reductase activity. Activity increases linearly from above 30 degrees Celsius to reach maximal levels at 80 degrees Celsius and then decreases to 15% of activity at 90 degrees Celsius.
Optimum pH is 6.0. Stable between pH 4.0 and 10.0 for 120 minutes.
Optimum temperature is 40 degrees Celsius. Stable up to 40 degrees Celsius.
Optimum pH is 6.5 for sulfide production.
Optimum pH is 4.5-5.0.
Optimum pH is 8.5 for oxidation and 7.0 for reduction.
Optimum temperature is 75 degrees Celsius for both ethanol oxidation and acetaldehyde reduction.
Optimum pH is 6.3 at 60 degrees Celsius.
Optimum pH is 5.0. Active over a broad range of pH values.
Has maximum activity at 45 to 60 degrees Celsius. Inactive at temperatures of 70 degrees Celsius and above.
Optimum pH is 9.0 (with pNP-decanoate as substrate).
Optimum temperature is 40 degrees Celsius (with pNP-decanoate as substrate).
Optimum pH is 7.5 for quinuclidinone reduction. Optimum pH is 6.0 for 4-methylcyclohexanone reduction.
Highly thermostable, remains fully active after 2 hours at 80 degrees Celsius.
Optimum temperature is over 110 degrees Celsius.
Has two pH optima at 8 and 10.
The reductase activity toward cytochrome c increases at alkaline pH 8-9 when compared to pH 6-7.
Optimum temperature is above 70 degrees Celsius.
Optimum pH is 6.5 with Ins(1,4,5)P3 as substrate, 7.5 with PtdIns(3,4,5)P3 as substrate, and 6.5-8.5 with PtdIns(4,5)P2 as substrate.
Optimum temperature is 22 degrees Celsius with Ins(1,4,5)P3 as substrate, and 22-37 degrees Celsius with PtdIns(4,5)P2 and PtdIns(3,4,5)P3 as substrates.
Optimum pH is 9 for the ATP-independent peptide cleavage activity and for ATPase activity.
Optimum temperature is 70 degrees Celsius for the ATP-independent peptide cleavage activity, and 95 degrees Celsius for ATPase activity.
Optimum temperature is 83 degrees Celsius.
Optimum pH is 8.5 with pNP-butyrate as substrate. Shows 70% of the maximal activity at pH 7.0 and pH 9.5.
Optimum temperature is 50 degrees Celsius with pNP-butanoate as substrate (PubMed:22194294, PubMed:24593046). Optimum temperature using PET as substrate is superior to 70 degrees Celsius (PubMed:22194294). Shows 70% of the maximal activity at 30 and 70 degrees Celsius with pNP-butyrate as substrate. Has half-lives of 5 hours at 50 degrees Celsius, 80 minutes at 60 degrees Celsius, 40 minutes at 70 degrees Celsius and 7 minutes at 80 degrees Celsius (PubMed:22194294). Is thermostable, with a determined melting temperature (Tm) of 84.7 degrees Celsius (PubMed:32269349).
Optimum pH is 6-9. At pH 4 and pH 10, its activity is only 40 and 60%, respectively, of the maximum.
Optimum temperature is 45 degrees Celsius. However, at that temperature, MupP is completely inactivated within 40 minutes. Loses about 10% of its activity at 22 degrees Celsius and 70% at 37 degrees Celsius within 40 minutes.
Optimum temperature is about 25 degrees Celsius.
Optimum temperature is 70 degrees Celsius. The enzyme is stable for 30 minutes at temperatures up to 70 degrees Celsius.
Optimum pH is between 9 and 10.
Optimum temperature is between 23 and 25 degrees Celsius. 37% of this maximal activity could still be observed at 5 degrees Celsius.
Optimum pH is 7.8. Shows high pH stability over a wide range of pH (pH 4-12).
Optimum temperature is 70 degrees Celsius. Very stable at high temperature. 90% of the activity is retained after treatment at 90 degrees Celsius for 60 min.
Optimum pH is 5.5 for decarboxylase activity.
Optimum temperature is 40 degrees Celsius for the decarboxylase activity and 30 degrees Celsius for carboxylase activity.
Optimum temperature is 60 degrees Celsius. Thermostable.
Optimum pH is 5.5. Stable between pH 4.0 and 6.5.
Optimum pH is 2.0 and 7.5.
Optimum pH is 7.25.
Optimum pH is 5.0 with pNP-GlcNAc and 4.0 with pNP-GalNAc. Half maximal activity observed at pH 3.0 and 6.5 with pNP-GlcNAc and at pH 2.5 and 5.5 with pNP-GalNAc. Retains over 90% of its activity between pH 6.5-8.0 with pNP-GlcNAc, and between pH 6.0-7.5 with pNP-GalNAc.
Optimum temperature is 50 degrees Celsius toward pNP-GlcNAc and 60 degrees Celsius toward pNP-GalNAc. Displays activity higher than 50% between 35-70 degrees Celsius. Stable under 30 degrees Celsius.
Optimum pH is between 6 and 9.
Optimally active at alkaline pHs. Activity increases with increasing pHs.
Is thermostable. After incubation at 85 degrees Celsius for 30 minutes, more than 95% of I-1-Pase activity remains.
Optimum pH is 3.5-4.0.
Optimum pH for protease activity is 7.1 (PubMed:11210524, PubMed:9872409). Nanocompartments are stable from pH 4-12, they reversibly disassemble at pH 1 and pH 13 (PubMed:27224728).
Optimum temperature for protease activity is 90-93 degrees Celsius.
Optimum pH is 5.2. Active from pH 4.5 to 6.5.
Optimum pH is 6 for the forward reaction and 7.5 for the reverse reaction.
Active from pH 5.5 to 8.5.
Optimum pH is 8.0-9.5 in the presence of L-arginine, and 8.5-9.0 in the presence of 1 mM L-arginine.
Optimum temperature is 25-35 degrees Celsius in the presence of L-arginine, and 38-42 degrees Celsius in the presence of 1 mM L-arginine.
Optimum pH is 5.0 for mono- and diesters hydrolysis.
Optimum pH is 5-10.
Optimum pH is 4.2-5.2 for birchwood xylan and 4.0 for CM cellulose.
Optimum temperature is 10-30 degrees Celsius.
Optimum pH is 7.0. Stable between pH 5.0 and 8.0.
Optimum temperature is 37 degrees Celsius. Stable below 30 degrees Celsius.
Optimum pH is 8.5 for pNPP hydrolysis (PubMed:19016841). Optimum pH is 8.0 for pNPP hydrolysis (PubMed:19700407).
Optimum temperature is 37 degrees Celsius for phosphatase activity.
Optimum temperature is around 55 degrees Celsius. In the range from 37 to 55 degrees Celsius, the proteolytic activity rapidly increases with temperature.
Active at alkaline pH.
Optimum temperature is 16 degrees Celsius. Not active at temperatures above 55 degrees Celsius. Complete loss of activity by incubation at 55 degrees Celsius for 1 min.
Optimum pH is 7.0-8.0 for mature LGOX (Ref.2). Optimum pH is 7.0 for recombinant LGOX expressed in E.coli (PubMed:14769868).
Optimum temperature is 35 degrees Celsius at pH 7.4 and 50 degrees Celsius at pH 6.0 for recombinant LGOX expressed in E.coli.
Optimum pH is 7. Higher activity at pH 4 than at pH 7 in sperm.
Optimum pH is 7.5-9 (PubMed:11032404). Optimum pH is 6.5-7 (at 30 degrees Celsius) (PubMed:10626375).
Is stable for up to 48 hours at 80 degrees Celsius. Has a thermal denaturation midpoint (Tm) of 95.7 degrees Celsius.
Optimum pH is 8.0 for the NAD(+)-dependent oxidation of vanillin. Active between pH 5.0-9.0.
Optimum temperature is 44 degrees Celsius for the NAD(+)-dependent oxidation of vanillin. Active between 15-65 degrees Celsius. Retains 86% of the activity after storage at 4 degrees Celsius for 24 hours.
Optimum pH is 7.5-9.3 with dimethyl casein as substrate.
Optimum temperature is 30 degrees Celsius with dimethyl casein as substrate.
Optimum pH is between 8.5-9.5. No catalytic activity is detected below pH 7.5.
Optimum pH is 6-7 for N-hexadecanoylsphing-4-enine hydrolysis (PubMed:11328816). Optimum pH is 7-10 for N-hexadecanoylsphing-4-enine hydrolysis (PubMed:10488143). Optimum pH is 6-8 for N-(octanoyl)-sphing-4-enine hydrolysis (PubMed:15217782). Optimum pH is 6.5-7 for hexadecanoate in the reverse reaction (PubMed:11278489).
Optimum pH is 7.4-7.5.
Optimum pH is 4.5-7.5. Is almost completely inactivated at pH 11.5.
Optimum temperature is 25-60 degrees Celsius. All temperatures tested are optimal.
Thermostable. Still active at 100 degrees Celsius.
Optimum pH is 8.4 with L-xylulose as substrate.
Optimum pH is 7.5. Active from pH 6.8 to 10.
Optimum pH is 5.5-9.0.
The activity is gradually lost at temperatures of more than 30 degrees Celsius.
Optimum pH is 6.95 and 7.05 with lysoplasmenyl-ethanolamine and lysoplasmenyl-choline as substrates, respectively.
Optimum temperature is 23 degrees Celsius. 90% of maximal activity from 4 to 37 degrees Celsius.
Optimum pH is 7.0-10.0.
Thermostable. Optimum temperature is 70 degrees Celsius.
Optimum temperature is 37 degrees Celsius (with palmitoyl-CoA as substrate).
Optimum pH is 6-7. Active fom pH 5 to pH 9.
Optimum pH is 1-8.
Optimum temperature is 4-90 degrees Celsius.
Optimum pH is 7.8 for the synthetase reaction and pH 7.2 for the transferase reaction.
Optimum temperature is 60 degrees Celsius for both transferase and synthetase activities.
Optimum pH is 4.5 for oxidation of 2,6-dimethoxyphenol (PubMed:23111597). Retains 100% activity after incubation at pH 2.5 for 4 hours (PubMed:23111597, PubMed:19756587, PubMed:25153532). Retains >60% activity after incubation at pH 2-11 at 4 degrees Celsius (PubMed:25153532). Retains >60% activity after incubation at pH 3-9 at room temperature (PubMed:25153532).
Retains >80% activity after incubation at temperatures up to 60 degrees Celsius for 10 minutes. Retains 50% activity after incubation at 65.5 degrees Celsius for 10 minutes. Activity is lost after incubation at 70 degrees Celsius for 10 minutes.
Optimum pH is 8.0 for the cleavage of a 3'-flap structure.
Optimum pH is 6.6-7-4. Activity decreases gradually at pH over 7.4 or below 6.6.
Optimum pH is 7.0 for the reduction reaction and 8.5 for the oxidation reaction.
Optimum pH is 7.0 for the catalase activity and 4.5-5.5 for the peroxidase activity (PubMed:9006925). Optimum pH is 4.75 for the peroxidase activity (PubMed:18178143).
Optimum temperature is 32 degrees Celsius. Enzyme is stable up to 40 degrees Celsius.
Displays hemagglutinating activity from pH 5 to 8, but is inactive beyond these pHs.
Half the hemagglutinating activity is maintained by heating at 50 degrees Celsius for 3 hours. Is inactive by heating at 60 degrees Celsius for 10 minutes.
Optimum temperature is 60 degrees Celsius. The activity increases quite linearly with temperature and is nearly twice as high at 60 degrees Celsius as compared to 30 degrees Celsius. Above 60 degrees Celsius, the enzyme activity dramatically decreases, probably due to thermal inactivation.
Optimum pH is 5.5-6.2.
Optimum temperature is 80 degrees Celsius. Active up to 95 degrees Celsius.
Optimum temperature is 25 degrees Celsius when incubation is carried out at pH 7.0-9.0.
Optimum pH is 7.6-8 for carboxyesterase activity.
Fully active up to 55 degrees Celsius.
Optimum pH is 3.8 with 2,6-dimethoxyphenol as substrate, and less than 2.7 with ABTS as substrate.
Optimum temperature is 50 degrees Celsius. Half-life at 60 degrees Celsius is 20 minutes. Half-life at 70 degrees Celsius is 3 minutes.
Active at 80 degrees Celsius.
Optimum temperature is 35 degrees Celsius. Thermal denaturation midpoint (Tm) is 43.3 degrees Celsius and is raised to 60.4 degrees Celsius when AK is complexed with the inhibitor Ap5A.
Optimum pH is 9.0 (at 60 degrees Celsius).
Fairly stable at neutral or alkaline pH.
Inactivated at temperatures above 45 degrees Celsius.
Optimum pH is 7.5 for NADH -> NAD transhydrogenation, 6.5 for lipoamide reduction, 4.5 for NADPH -> NAD transhydrogenation, and 6.5 for NADH-ferricyanide reduction.
Optimum pH is 7.5, with half-maximal activity at pH 6.0 and 8.3.
Optimum temperature is 30 degrees Celsius with o-nitrophenyl-beta-D-galactopyranoside (ONPG) as substrate. Has high specific activity at low temperatures.
Optimum temperature is 50 degrees Celsius (PubMed:20304972). Unstable upon 65 degrees Celsius (PubMed:20304972).
Optimum pH is 10.7.
Thermostable. Highly active at 75-80 degrees Celsius.
Optimum pH is 7.4. It retains a high specific activity over a broad pH range.
Optimum pH is 5.5-6.
Optimum temperature is 102 degrees Celsius. Thermostable.
Thermostable up to 70 degrees Celsius.
Optimum pH is between 7 and 7.2.
Optimum temperature is between 85 and 95 degrees Celsius.
Optimum pH is 7.0. Stable from pH 7.5 to 8.5.
Optimum temperature is 35 degrees Celsius. Inactive following 30 minutes incubation at 50 degrees Celsius or above, no loss of activity following 30 minutes incubation at 25 degrees Celsius or below. Little loss of activity following 30 minutes incubation at 35 or 30 degrees Celsius.
Optimum pH is about 7.
Optimum pH is 8.5 with oxaloacetate as substrate.
Optimum temperature is 60 degrees Celsius with oxaloacetate as substrate.
Optimum temperature is 60 degrees Celsius. Does not lose activity after 120 minutes at 60 degrees Celsius at pH7 with 50 uM of manganese chloride and 5 mM of magnesium chloride.
Optimum pH is 7. Active from pH 5 to pH 11.
Optimum temperature is 60 degrees Celsius. Enzyme activity decreases sharply above 63 degrees Celsius.
Threonine dehydratase 2 biosynthetic, chloroplastic: Active at alkaline pH (PubMed:21436043, PubMed:17416643). Processed threonine dehydratase 2: Optimum pH is 9. Has little or no activity at pH values below 6.0 (PubMed:17416643).
Threonine dehydratase 2 biosynthetic, chloroplastic: Optimum temperature is 60 degrees Celsius. Abnormally stable at elevated temperatures (PubMed:21436043). Processed threonine dehydratase 2: Active over a wide range of temperatures. Optimal enzyme activity against L-threonine is at 58 degrees Celsius (PubMed:17416643).
Optimum pH is 6.5. Retains more than 50% of maximal activity from pH 5.0 to 8.0.
Optimum temperature is 65 degrees Celsius. Highly thermostable at 80 degrees Celsius.
Optimum pH is 4-7.
Optimum pH is 9.2. Retains 66% of maximum activity at pH 8.6 and 57% of maximum activity at pH 10.0.
Optimum temperature is 50 degrees Celsius. 37% and 33% of maximum activity is seen at 30 and 55 degrees Celsius respectively. Stable for 10 minutes at 45 degrees Celsius or lower, 76% of activity remains following 10 minutes incubation at 50 degrees Celsius and 1.3% of activity remains following 10 minutes incubation at 60 degrees Celsius.
Optimum pH is 7.0. 50% of activity is found at pH 6.1 and 8.2.
Optimum temperature is 85 degrees Celsius. Thermostable.
Activity is independent of pH in the range of pH 5-9.
Thermostable. The midpoint for its thermal unfolding transition (Tm) is 88 degrees Celsius.
Optimum temperature is 30 degrees Celsius (at pH 5.3).
Optimum pH is 6.0, enzymatic activity becomes unstable below this value.
Activity is slightly enhanced at pH 6.6 and pH 6.0. Retains only 10% of its maximal activity at pH 7.6 and is inactive at pH 8.0.
Optimum pH is 6.5-10.5.
Optimum pH is 7.0. Stable in the range of pH 5.5-8.0.
Stable up to 55 degrees Celsius.
Optimum pH is 6.5 with thermospermine as substrate (PubMed:24906355). Optimum pH is 7.5 with spermine as substrate (PubMed:24906355).
Optimum temperature is 40-45 degrees Celsius with thermospermine as substrate (PubMed:24906355). Optimum temperature is 35-45 degrees Celsius with spermine as substrate (PubMed:24906355).
Optimum temperature is 63 degrees Celsius.
Optimum temperature is above 92 degrees Celsius.
Optimum temperature is over 75 degrees Celsius for DNA cleavage, DNA-binding occurs below that.
Retains hemagglutinating activity after heating at 50 degrees Celsius for 120 minutes. Activity is abolished by heating at 80 degrees Celsius for 90 minutes.
Active between pH 5 and 10.
Active between 10 and 60 degrees Celsius.
Optimum pH is 5.0 for both endo-beta-mannanase and mannan transglycosylase activities.
Optimum pH is 6.0 for activities against both pNPAp and pNPGp. Stable in the range of pH 6.0-8.0.
Optimum temperature is 30-40 degrees Celsius for activities against both pNPAp and pNPGp when incubated 10 minutes at pH 6.0. Both activities completely lost after heating at 50 degrees Celsius for 20 minutes.
Optimum pH is 5.5. Cytolytic activity is undetectable at pH 7.0.
Optimum pH is 8.0 in MOPS buffer at 30 degrees Celsius, 7.5 in 0.1 M potassium phosphate buffer at 30 degrees Celsius, and 7.4 in 20 mM potassium phosphate/2 mM DTT buffer at 30 degrees Celsius. Retains full activity in MOPS buffer between pH 6.5 and 10.0. Is irreversibly damaged below pH 6.5 in MOPS buffer, enzyme treated at pH 6.0 in MOPS buffer only regains 40% of its original activity after re-equilibration at pH 7.2. When treated at pH 5.2 or 10.1 in 20 mM potassium phosphate/2 mM DTT buffer activity is lost completely, but is recovered within 17 minutes following readjustment to pH 7.4.
Optimum temperature is 45 degrees Celsius. Activity is lost after 5 minutes incubation at 60 degrees Celsius, but one-fifth of this activity is restored after cooling to 45 degrees Celsius.
Thermostable up to 53 degrees Celsius.
Optimum pH is 6.35.
Optimum pH is 7-8. Active from pH 5.0 to 8.5.
Optimum pH is 7.6. Decreased activity at pH values below 7.0 and above 8.0. The activity against chondroitin 6-sulfate remains higher than with other substrates at low pH. At pH 6.5 the enzyme exhibits almost 60% of its maximal activity against chondroitin 6-sulfate, only 20% activity against chondroitin 4-sulfate and no measurable activity against dermatan sulfate. In contrast, at pH of 8.5 about 30% of enzyme's maximal activity against all substrates is displayed.
Optimum temperature is 37 degrees Celsius. No significant reduction in activity at temperatures in the range of 25-40 degrees Celsius. At 50 degrees Celsius, activity of 45% for dermatan sulfate, 60% for chondroitin 4-sulfate and 75% for chondroitin 6-sulfate is detected. Thermal denaturation curve is bimodal with two consecutive thermal denaturation midpoints (Tm) corresponding to 44 and 50 degrees Celsius, respectively.
Optimum pH 3.0. Highly stable at acidic pH.
Optimum pH is 7.5-9.0 with (R)-3-hydroxybutanoate and 6.5-7.0 with 4-oxo-L-proline.
Optimum pH is 8.0 for the coupled WbpB/WbpE reaction.
Optimum temperature is 30 degrees Celsius for the coupled WbpB/WbpE reaction.
Optimum pH is 5.0-5.5. Inactive at neutral pH.
Shows at least 80% of maximal activity from pH 5.9 to greater than 7.8 for acetate conversion.
Optimum pH is 7.3-7.8.
Optimum pH is 5 for ubiquinol oxidation activity.
Optimum pH is around 5, the pH range of activity varies in tested buffers.
Increasing activity in the range between 20 and 92 degrees Celsius.
Optimum pH is about 6 using pNPP as substrate. Retains more than 50% of maximal activity in the pH range 4-7.
Optimum pH is 7.4 with glutamine as substrate (PubMed:21413787). Optimum pH is 9.2 with NH4(+) as exogenous source of ammonia (PubMed:21413787).
Optimum pH is 8-9. Active from pH 5.5 to pH 10.
Optimum pH is 8. Activity is highly sensitive to pH variation, and an increase or decrease of one pH unit from the optimal attenuates more than 70% of activity. At pH 6.0, only 6% of the activity remains. At pH.5.0, the activity is completely abolished.
Optimum temperature is 37 degrees Celsius. It appears to be stable between 20 and 50 degrees Celsius but becomes very unstable at or above 50 degrees Celsius.
Stable at pH values ranging from 3.0-11.0 at 4 degrees Celsius.
Thermostable when incubated at pH 8.0 at temperatures between 40 and 100 degrees Celsius.
Optimum temperature is 37 degrees Celsius. Stable at 30 degrees Celsius. More than half of its activity is lost after 30 min of treatment at 35 degrees Celsius. Activity is lost by 30 min of treatment above 40 degrees Celsius.
Optimum pH is 7.8 (for O-acetylhomoserine sulfhydrylase activity in Tris-HCl buffer) and 8.4 (for both O-acetylhomoserine sulfhydrylase and O-acetylserine sulfhydrylase activities in barbital-HCl buffer).
Highly thermostable. Exhibits over 85% or 60% of activity after a 1 hour or 3 hours incubation at 90 degrees Celsius, respectively. The half-life is estimated to be 257 minutes.
Optimum pH is 7.0-7.2.
Optimum pH is 7.5-8.8 for L-Leu.
Optimum pH is 8-8.5 at 37 degrees Celsius.
Optimum pH is 9.0 for lipolytic activity of the full-length protein.
Optimum temperature is 40 degrees Celsius for lipolytic activity with pNP-decanoate as substrate (PubMed:33960821). Optimum temperature is 45 degrees Celsius for adenylate cyclase activity (PubMed:15678099).
Thermostable. Retains 75% of its activity after heating 30 minutes at 96 degrees Celsius.
Optimum pH is 6.5 for the ferric reductase activity.
Optimum temperature is 35 degrees Celsius for the ferric reductase activity.
Optimum pH is 7.1. The trehalase activity drops sharply at pH values of 6.5 and below, as well as at pH values of 8.0 and above.
Activity is stable up to 60 degrees Celsius, then decreases and is lost at 80 degrees Celsius.
Optimum pH is 4.3. Active from pH 3 to pH 7.0.
Optimum pH is 6.0. Stable from pH 4.5 to 6.5.
Optimum pH is 7.0 with acetophenone as substrate.
Optimum temperature is 50 degrees Celsius with acetophenone as substrate.
Optimum pH is 6.0 for reductase activity.
Optimum pH is 8.0-8.5 for transfer of p-nitroanilide from gamma-glutamyl-p-nitroanilide (gamma-GpNA) to glycylglycine (gly-gly).
Optimum pH is 4.0-4.5.
Loses rapidly its activity at temperatures above 30 degrees Celsius.
Stable for 15 minutes at up to 80 degrees Celsius.
Antibacterial activity retained between pH 7 and 10.
Antibacterial activity retained between 10 and 70 degrees Celsius.
Optimum pH is 7.2-8.8 for the propionyl-CoA carboxylase activity as measured for the holoenzyme.
Optimum pH is 8.6-8.7.
Optimum pH is 8 (PubMed:15555935). Optimum pH is 8.5 (PubMed:7592838). Optimum pH is 11-12 (PubMed:8987657).
Optimum temperature is 60 degrees Celsius (PubMed:15555935, PubMed:8987657). Optimum temperature is 50 degrees Celsius (PubMed:7592838).
Optimum pH is 8.0 for PDH complex activity. Half-maximal activity is observed at pH 7.0 and pH 9.0. Activity is abolished at pH < 5.
Optimum pH is 7.0. Stable at pH 6.5-8.2.
Optimum temperature is 60 degrees Celsius. Very thermostable as still has 98% of its activity after incubation for 30 minutes at 50 degrees Celsius.
Optimum pH is 5.3-6.3.
Optimum pH is between 6-8. Outside of this pH range, the enzyme activity decreases rapidly. At pH 4.6, the enzyme is only 7% as active as it is at pH 7.5.
Optimum pH is 8.0 for the hydrolysis of p-nitrophenyl butyrate.
Optimum temperature is 38 degrees Celsius for the hydrolysis of p-nitrophenyl butyrate.
Optimum temperature is 40 degrees Celsius. Thermostable from 20 to 50 degrees Celsius.
Active at pH 7 (up to 40 degrees Celsius for 30 min) and at pH 11 (up to 25 degrees Celsius).
Protease activity is retained up to 40 degrees Celsius for 30 min, but completely inactivated at 50 degrees Celsius (at pH 7). Activity is retained up to 25 degrees Celsius, and completely inactivated at 40 degrees Celsius (at pH 11).
Optimum pH is 8.5. Inactive at a pH below 6.5.
Optimum temperature is 27 degrees Celsius.
Optimum pH is 9.0 (PubMed:8106500) or pH 7.0-9.0 (PubMed:8195150).
Optimum pH is 8.0 for the hydrolysis of Gly-Pro-pNA. No hydrolysis is detected at a pH below 5.5 or above 11.0.
Optimum temperature is between 40 and 50 degrees Celsius for the hydrolysis of Gly-Pro-pNA. Stable for at least 30 min below 30 degrees Celsius.
Optimum pH is pH 5.5-5.
Optimum pH is 3.0-4.0. Highly stable at acidic pH.
Optimum pH is 7.5 (PubMed:25037224, PubMed:25521849). Activity declines progressively before pH 7.5 and is almost abolished at 6.0 while at higher pH the enzyme remains active till pH 9.0 (PubMed:25521849).
Optimum temperature is 37 degrees Celsius. Activity declines at higher temperatures and is completely abolished by 50 degrees Celsius.
Optimum pH for cob(III)alamin reductase activity is pH 9.5, no major effect of pH was seen on cob(II)alamin reductase activity between pH 7-10.
Optimum temperature is 37 degrees Celsius for cob(III)alamin reductase and 42 degrees Celsius for cob(II)alamin reductase.
Optimum pH is 6-6.5. Retains more than 70% of the maximum agglutinating activity over the pH range 5-9.
Unchanged hemagglutinating activity after 30 minutes incubation at temperatures up to 50 degrees Celsius, then at higher temperatures activity decreases. 14% of the initial activity is retained even after heating at 100 degrees Celsius.
Active from pH 6.0 to 10.0.
Activity decreases at temperatures above 55 degrees Celsius.
Optimum temperature is 40 degrees Celsius (PubMed:24714029). Active from 5 to 50 degrees Celsius (PubMed:24714029).
Optimum pH is 9.0-10.
Very sensitive to pH. The enzyme activity rises 25-fold between pH 6 and 8.
Addition of manganese increases the thermal stability.
Optimum pH is 7 for the hydrolysis of xylotriose.
Optimum temperature is 40 degrees Celsius for the hydrolysis of xylotriose.
Stable over a wide pH range, activity is unaffected after incubation at pH 2.5-10.5.
Activity is unaffected by heating at 60 degrees Celsius for 1 hour. Activity gradually decreases after incubation at higher temperatures.
Thermostable. Has a melting point (Tm) of 85.5 degrees Celsius.
Optimum pH is 4.0 for the peroxidase reaction.
Optimum pH is 8.5 at 60 degrees Celsius.
Optimum temperature is above 80 degrees Celsius.
Optimum temperature is 32 degrees Celsius (PubMed:17636255, PubMed:24714029). Active from 5 to 50 degrees Celsius (PubMed:24714029).
Optimum pH is 8.0 for decarboxylation. Optimum pH is 7.0 for carboxylation.
Stable from 20 degrees Celsius to 70 degrees Celsius. Activity decreases sharply above 70 degrees Celsius.
Active in pH ranges of 3.5-10.5.
Retains over 70% of its activity after heating at 90 degrees Celsius for 10 min.
Optimum pH is 4.5-5.0. The enzyme from cv. GT.1 displays a second optimum pH at 6.7.
Optimum temperature is superior to 90 degrees Celsius. Thermostable. Retains full activity after 3 hours at 70 degrees Celsius.
Optimum pH is 6.6. Maintains activity over a broad range of pH values from 6 to 9.
Optimum temperature is 45-50 degrees Celsius. Activity declines rapidly above 50 degrees Celsius. Stable for at least 70 hours at temperatures 35 degrees Celsius and below. At 50 degrees Celsius loses all activity in less than 15 minutes.
Active from 70 to 90 degrees Celsius.
Optimum pH is 5.2-6.5.
Thermostable. Retains 85-90 % of its activity after 3 hours of incubation at 90 degrees Celsius.
Optimum pH is 6.3. 50% remaining activity is observed at pH 5.3 and pH 7.
Optimum temperature is 89 degrees Celsius. It does not lose activity upon incubation at 80 degrees Celsius for about 120 min and still has a half-life at 95 degrees Celsius of 40 min. At 100 degrees Celsius, an almost complete loss of activity is observed after 30 min.
Stable at pH 3.
Stable up to 90 degrees Celsius.
Optimum pH is 8.5. No activity at pH below 6.5 or above 9.5.
Optimum pH is 5 for xylosyltransferase activity. Optimum pH is from 5.5 to 8.0 for Beta-1,3-glucuronyltransferase activity.
Optimum pH is 7.0 and half of the maximum activity remains at pH 6-10.
Optimum temperature is 45 degrees Celsius. It has strong activity at moderate temperature 30-50 degrees Celsius and about half of the maximal activity is retained at 100 degrees Celsius.
Loses its activity after heat treatment.
Optimum pH is 8.0 with L-cystathionine.
Optimum pH is pH 8.5 (NAD reduction, bile acid oxidation) and 6.5 (NADH oxidation, bile acid reduction).
Is relatively thermostable, retaining 95% of initial activity after 1 hour at 65 degrees Celsius.
Optimum pH is 5.5. High catalytic activity is observed between pH 5.5 and 7.0.
Optimum pH is 5.0-6.0. Half maximum activity is seen at pH 3.5 and 6.5.
Optimum pH is 8.2 with L,L-cystathionine.
Optimum pH is 7.4. Active from pH 6.7 to 8.5.
Optimum temperature is 55 degrees Celsius (PubMed:33737179). Exhibits a half-life of 2 hours at 80 degrees Celsius.
Active over a wide pH range between 5 and 9.
Optimum pH is 8.2-8.4.
Optimum pH is 9.75 with 4-aminobutanal as substrate. Optimum pH is 9.5 with 3-aminopropanal as substrate.
Optimum temperature is 15 degrees Celsius.
Optimum pH is 9 (PubMed:16681462). Active between pH 7-10 (for alanine and glyoxylate as substrates) (PubMed:16681462).
Optimum temperature is 60 degrees Celsius (PubMed:16681462). Maximum activity between 50 and 80 degrees Celsius (for alanine and glyoxylate as substrates) (PubMed:16681462).
Optimum pH is 6.5-7.7 for the conversion of 2-phosphoglycerate to phosphoenolpyruvate (PubMed:15794763). Optimum pH is 6.0 for the conversion of phosphoenolpyruvate to 2-phosphoglycerate (PubMed:15794763).
Optimum pH is >9.5.
L-dopa decarboxylation occurs more rapidly at lower pH, suggesting that this metabolism is likely accelerated at the lower pH of the upper small intestine.
Optimum pH is 8.0. Active from pH 6.5 to 10.
Optimum temperature is 70-75 degrees Celsius.
Optimum pH is above 10 (at 80 degrees Celsius).
Optimum temperature is above 90 degrees Celsius (at pH 8.4).
Optimum pH is 4.35.
Optimum temperature at pH 7.5 is 40 degrees Celsius with no activity at 50 degrees Celsius.
Optimum temperature at pH 7.5 is 47.5 degrees Celsius with 57% activity retained at 50 degrees Celsius.
Optimum pH is between 7.2 and 7.8.
Highly thermostable. Not degraded by heating at 140 degrees Celsius for 20 min.
Optimum pH is 7.5-8.5. Strongly inhibited at acidic PH.
Optimum pH is 6.5-7.
Optimum pH is 7.0 for cytosolic pH.
The protein is very stable, after 18 months at -20 degrees Celsius it retains 90% of its activity.
Optimum temperature is 35 degrees Celsius. Retains significant activity in the low temperature range (less than 10 degrees Celsius).
Optimum pH is 9.0 for oxidation of Fe(2+).
Optimum pH is around 8.5.
Optimum temperature is 80 degrees Celsius. Extremely thermostable.
Optimum temperature is 32 degrees Celsius (PubMed:24714029). Active from 5 to 47 degrees Celsius (PubMed:24714029).
Remains fully active after heating 15 minutes at 85 degrees Celsius. Completely inactive after 15 minutes of pre-incubation at 95 degrees Celsius.
Optimum pH is 7.0-7.6. Stable from pH 5.0 to 10.0. Rapidly inactivated below pH 4.5.
Optimum pH is 9.0 (at 80 degrees Celsius).
Optimum temperature is above 90 degrees Celsius (at pH 8.0).
Optimum temperature is 40 degrees Celsius (PubMed:9083041). Optimum temperature is 37 degrees Celsius (PubMed:18849565).
Optimum pH is 5-9.5.
Optimum pH is 6.5 with estrone 3-sulfate, taurocholate and prostaglandin E2 as substrates.
Optimum pH 8.0.
Maximum activity at 42 degrees Celsius. Stable at temperatures up to 44 degrees Celsius, showing 50% of activity after incubation at this temperature for 15 minutes (at pH 8.0).
Optimum pH is around 10. Below and above pH 10, the marked decrease of activity is observed: the relative activities are 50, 22 and 55% at pH 9.5, 9.2 and 12, respectively. Is stable over a wide pH range: upon heating at 50 degrees Celsius for 20 minutes, the enzyme does not lose activity at pH 4.5-10.0.
Optimum tempreature is 70 degrees Celsius. Extremely thermostable, the activity is not lost after incubation at 100 degrees Celsius for 20 minutes.
Optimum temperature is 60 degrees Celsius. Is extremely thermostable. No detectable activity is lost when the enzyme is incubated at 65 degrees Celsius for 4 hours.
Enolase activity is lost above pH 9.0. Immunoglobulin production stimulating activity is retained at pH 13.0.
Optimum pH is 5.0 for NADPH-methemoglobin reductase activity, and 6.5 for NADPH-diaphorase activity.
Stable from pH 1 to 10.
Thermostable. Retains 100% of its maximal activity after heating at 100 degrees Celsius for 15 min, but only 50% of activity after heating 1 hour at this same temperature.
Optimum pH is 7.0. Extremely stable over a wide range of pH levels.
Optimum temperature is 60-80 degrees Celsius.
Thermophile. Is much more active at 60 degrees Celsius than at 30 degrees Celsius.
Optimum pH is 6.6-8.0.
Optimum pH is 8.0-8.5 (PubMed:17071761). Optimum pH is 7.0-8.0 (PubMed:30392152).
Optimum temperature is 100 degrees Celsius. Inactive below 40 degrees Celsius. Extremely thermostable.
Optimum pH is 9.4 for the L-lysine adding activity.
Optimum temperature is 68 degrees Celsius for the L-lysine adding activity.
Optimum pH is 9.5 and 9.6-9.5 with poly(P)(32) and ATP as the phosphoryl donors, respectively.
Optimum temperature is 50 degrees Celsius for both activities.
Optimum pH is 3.8-5.0 for the hydrolysis of N-dodecanoylsphing-4-enine (PubMed:7744740, PubMed:12815059). Optimum pH is 5.5-6.5 for the synthesis of N-dodecanoylsphing-4-enine (PubMed:12815059).
Optimum pH is 6.5. Only 13% of activity is retained at pH 7.5.
Optimum temperature is 90 degrees Celsius. 65% of activity remains at 100 degrees Celsius.
Optimum pH is 7.5-7.6.
Optimum pH is 6.0-6.5 when making H(2) and 7.5 in the other direction.
Optimum pH is 10. Is active in a broad pH range, namely, between pH 6.0 and 10.5.
Optimum temperature is 80 degrees Celsius. Is active in a broad temperature range, namely, between 50 and 100 degrees Celsius. Thermostable.
Optimum pH is 7.0 for phosphorolytic activity. Optimum pH is 6.0 for the synthetic reaction.
Optimum temperature is 50 degrees Celsius. Activity decreases when incubated at temperatures above 24 degrees Celsius for 10 minutes. No activity remains after 10 minutes at 65 degrees Celsius.
Optimum pH is 4-6. Active from pH 3 to 7.
Unstable above 30 degrees Celsius.
Optimum pH is 7.4-8.0 for the clotting activity.
Active at pH 4 and 7.4.
Stable enzymatic activity between pH 7.0 and pH 9.0. Significant decrease in activity with pH lower than 7 and pH greater than 9.0.
Highest enzymatic activity at temperatures between 30 and 40 degrees Celsius. Activity is decreased greatly above 45 or below 25 degrees Celsius.
Optimum temperature is 45 degrees Celsius. Stable up to 50 degrees Celsius.
Optimum pH is 9.1.
Retains HJ-binding ability after an hour at 90 degrees Celsius.
Optimum temperature is 58 degrees Celsius.
Has high activity over a broad pH range (4 to 11.2).
Optimum temperature is 55 degrees Celsius. Displays a 2-fold higher activity at 55 degrees Celsius than at 30 degrees Celsius.
Optimum pH is 8.0 (for polysulfide as substrate) and 10.3 (for benzyl viologen as substrate).
Optimum temperature is 80 degrees Celsius. Has a half-life of 12 h at 95 degrees Celsius. Activity increases by 50% after incubation for several hours at 82 degrees Celsius.
Optimum pH is 10.5 for the dehydrogenase activity. Optimum pH is 6 for the reductase activity.
Stable at 100 degrees Celsius for 10 min.
Optimum pH is <7. Is active against E.coli ATCC25922 at pH 5 (MIC=50 uM), whereas is not active at pH 7 (MIC>200 uM).
Highly active between pH 6.5 and 9.0. Retains 50% and 10% of maximum activity at pH 5.0 and 4.5, respectively.
Optimum pH is 7.5-9 with ferricenium ion (Fc(+)) as electron acceptor. Optimum pH values vary between 2 and 10 depending on the electron acceptor (with D-glucose as substrate).
Optimum temperature is 75 degrees Celsius with ferricenium ion (Fc(+)) as electron acceptor.
Optimum pH is between 4.5 and 5.
The MNO activity increases with temperature up to 55 degrees Celsius.
Optimum temperature is 40 degrees Celsius. Activity rapidly decreases within 30 minutes of incubation at 90 degrees Celsius.
Optimum pH is 6.0-8.0. No activity below pH 3.0 or above pH 11.0.
Thermostable. Activity unaffected after incubation for 30 minutes at 30-70 degrees Celsius. Retains 50% of its maximal activity after incubation at 80 degrees Celsius for 30 minutes.
Optimum pH is between 7.5.
Optimum pH is 7.5-8.0 (for arachidonate 12-lipoxygenase activity).
Highly active from 35 to 95 degrees Celsius. Thermostable.
Optimum pH is 7.0 with both ethanolamine and choline as substrates. Fifty percent of maximal velocity occurs at pH 5.5 and pH 8.
Optimum pH is 7.5-8.0. Stable between 7.5-10.0.
Optimum temperature is 37-40 degrees Celsius. Stable below 37 degrees Celsius.
Optimum pH is 7.5- 8.5.
Optimum temperature is 40-42 degrees Celsius.
Optimum pH is 7-8 with ssDNA as substrate (PubMed:22506810, PubMed:23620482). Optimum pH is 6 with RNA as substrate (PubMed:22506810). Optimum pH is 5.5 with dsDNA as substrate (PubMed:23620482).
Optimum temperature is 70 degrees Celsius. Low activity at temperatures below 30 degrees Celsius. Above 70 degrees Celsius, the catalytic activity declines.
Optimum pH is 7.0 for dimethylcasein with a complete loss of activity above pH 11.0.
Optimum temperature is 30-40 degrees Celsius with a complete loss of activity above 60 degrees Celsius.
Optimum pH is 6.0 with acetylated xylan as substrate. Is active at acidic pH ranging from 5.0 to 6.0.
Optimum temperature is 50 degrees Celsius with acetylated xylan as substrate. Retains about 80% of the activity at 30-55 degrees Celsius.
Optimum pH is 6.2-7.3. Stable between pH 5.0 and 9.8 for 30 min at 30 degrees Celsius.
Optimum temperature is 50 degrees Celsius. Stable up to 40 degrees Celsius.
Optimum pH is 6.0. The enzyme is stable between pH 6.0 and 7.0.
Optimum temperature is 45 degrees Celsius. Unstable at 40 degrees Celsius with a half life of less than 60 min at 40 degrees Celsius and 10 min at 50 degrees Celsius.
Optimum pH is 7-9 in a mixed-buffer system. In a Tris buffer high levels of activity were detected at very alkaline pHs.
Optimum pH is 7-10 for nitrite reduction, and the optimum pH is 7 for sulfite reduction with only 20% residual activity at pH 7.8.
Optimum pH is 6.75-7.75.
Optimum temperature is 70 degrees Celsius, active between 37 and 90 degrees Celsius.
Optimum pH is about 9.4 with propionaldehyde as substrate, and 7.5 with 4-aminobutyraldehyde as substrate.
Optimum pH is 5.4 for the reaction with tetrahydroxychalcone and 5.0 - 7.0 for the reaction with pentahydroxychalcone.
Optimum temperature is 37-50 degrees Celsius using butyraldehyde as substrate.
Optimum temperature is 40-65 degrees Celsius.
Optimum temperature is 98 degrees Celsius. Thermostable.
Optimum pH is 6.0 for xylanase activity.
Optimum pH is 6.0. Displays less than 20% of Vmax at both pH 5.0 and pH 7.0.
Optimum pH for casein is 7.6 and philibertain g 1 retains more than 80% of maximum activity between pH 6.7 and pH 8.7, and 50% of maximum activity between pH 6.1 and pH 9.8. Optimum pH for PFLNA is 6.2-7.2 and philibertain g I retains more than 80% of maximum activity between pH 5.8 and pH 7.8, and 50% of maximum activity between pH 5.2 and pH 8.6.
Optimum pH is 4.5 to 6.0.
Optimum pH is 6.7 to 7.0.
Optimum pH is 7.5. Half of activity at pH 6.5 and 8.5.
Optimum pH is 6.5, more than 80% active between pH 5.0 and 7.0.
Optimum temperature is 60 degrees Celsius, more than 80% of activity remains after 1 hour at 60 degrees Celsius.
Optimum pH is 7.0 with 1,2-dioctanoyl-sn-glycerol as a substrate, and 7.4 with 1,2-dihexanoyl-sn-glycerol as a substrate.
Almost completely inactivated after 10 minutes at 50 degrees Celsius.
Optimum pH is 5.5. Shows 25% of the optimal activity at pH 4.6 and 7.0.
Optimum pH is 8.0 for cleavage of a 3'-flap structure.
Optimum temperature is 40 degrees Celsius. Above 45 degrees Celsius, the enzyme is denaturated.
Optimum pH is 8.5. Active from 5.5-10.5.
Optimum temperature is 30 degrees Celsius. Stable at 4 degrees Celsius with minimal loss of activity.
Optimum pH is 9 using spermidine and sinapoyl-CoA as substrates.
Optimum temperature is 80 degrees Celsius. Thermostable.
Optimum temperature is 30 degrees Celsius. The activity completely disappears after treatment at 65 degrees Celsius for 10 min.
Displays in vitro half-lives of 73, 70, and 48 hours at 26, 30, and 37 degrees Celsius, respectively, indicating it is a relatively stable enzyme.
Optimal transport of arginine at pH 5.
Optimum pH is 8-11. Activity decreases rapidly at values above pH 11 and below pH 8. Is inactive at pH 4.3.
Optimum pH is 9.2 for UPRTase activity.
Optimum temperature depends on the study: 5-38 degrees Celsius (PubMed:17320169) and 60 degrees Celsius (PubMed:30534149).
Moderately thermostable. Has a 1.5 hour half-life at 55 degrees Celsius.
Optimum temperature is 37 degrees Celsius. Inactive above 60 degrees Celsius.
Optimum pH for NAADP(+) production is 5.0 from either 2'-phospho-cyclic ADP-ribose or NADP(+), activity is much higher at pH 5.0 than 7.4.
The catalytic activity is essentially constant between pH 6.8 and 8.0.
Thermostable. Heating at 70 degrees Celsius for 180 minutes gives no observable loss of enzymatic activity.
Optimum pH is 6.0-7.3.
Optimum pH is 5.0. Stable between pH 4.0 and 6.0.
Highly thermostable. Retains over 60% of the activity after 7 hours incubation at 90 degrees Celsius.
Optimum pH is 6.0 or lower.
Optimum pH is 5-6 and pH 6-8 for papain and bovine cathepsin B, respectively.
Stable between 20-40 degrees Celsius.
High activity at a wide pH range between 7.0-11.5 at 20 degrees Celsius and pH 7.5. It is not fully stable at pH 6 or under and at pH 12 or over. It loses over 85% of its activity at pH 3 or under and at pH 13.
Optimum temperature is about 100 degrees Celsius. Highly thermostable. Stable at 80 degrees Celsius for at least 3 hours. Half-life at 100 degrees Celsius is 100 minutes and at 90 degrees Celsius more than 3 hours.
Optimum pH is about 9.0.
Optimum pH is 8.3 (in the presence of Tris-HCl). Active from 7.3-7.8 in the presence of other buffers.
Not stable at 0 degrees Celsius. Decrease in activity up to 40% immediately after freeze-drying procedure, with a further decrease up to 10% when stored at -10 degrees Celsius.
Optimum pH is 5.8 for acetylxylan esterase activity, and 5.8 for endo-1,4-beta-xylanase activity.
Optimum temperature is 58 degrees Celsius for acetylxylan esterase activity, and 49 degrees Celsius for endo-1,4-beta-xylanase activity.
Optimum pH is 5.6. Active from pH 5 to 7.5.
Activity is stable between pH 2 and 12 at 25 degrees Celsius.
Thermostable in a pH-dependent manner. Retains 100% activity after 15 minutes at 121 degrees Celsius at pH 4. Retained activity is lower when incubated at basic pH or pH 2.
Optimum temperature is around 90 degrees Celsius. Extremely thermostable.
Optimum pH is between 6 and 6.5.
Optimum pH is 7.0 with DV-Chlidea or DV-Pchlidea as substrate, optimum pH is 6.5 with DV-Chla, DV-MPE or DV-Mg-Proto as substrate.
Optimum temperature is 25 degrees Celsius with DV-Chlidea or DV-Chla as substrate, Optimum temperature is 20 degrees Celsius with DV-Pchlidea, DV-MPE or DV-Mg-Proto as substrate.
Optimum pH is 8.0. Retains over 50% of its activity after treatment for 20 min between pH 7.0 and 11.0. Inactivated by treatment at pH 13.0 and at pH values less than 5.0.
Optimum temperature is 55 degrees Celsius. Stable from 25 to 40 degrees Celsius. Activity is reduced considerably by incubation above 40 degrees Celsius for 20 min.
Optimum pH is about 7.0 for both the deamination and amination reactions.
Optimum temperature is 82 degrees Celsius for the reductive amination of pyruvate. Retains 30% of its maximum activity at 25 degrees Celsius. Completely loses its activity when incubated at 90 degrees Celsius for 2 hours. The thermostability of the enzyme is increased by more than 10-fold by 1.5 M KCl to a half-life of 55 hours at 90 degrees Celsius.
Optimum temperature is 27-32 degrees Celsius.
Optimum pH is 6.1 for the reduction of succinic semialdehyde and pH 9.4 for the oxidation of 4-hydroxybutanoate.
Optimum pH for both PC and PE is between 7.0 and 7.5.
Optimum temperature is about 27 degrees Celsius. Loses activity at 41 degrees Celsius.
Optimum pH is 8.0-9.0 for nuclease activity.
Optimum temperature is 37 degrees Celsius for nuclease activity (PubMed:27206388). Activity reduced significantly beyond 45 degrees Celsius (PubMed:27206388).
Optimum pH is 7.0 (at 40 degrees Celsius).
Optimum pH is 8.0. Stable between pH 6.0 to 9.0.
Optimum pH is between 6.5 and 7.5 for nonanal as substrate.
Optimum pH is 4 (for phospholipase activity).
Optimum pH is 7.5. Active from pH 5.0 to 9.0.
Optimum temperature is 45 degrees Celsius. Active from 30 to 60 degrees Celsius.
Optimum temperature is 60 degrees Celsius. Thermostable. Retains 70% of its activity after heating at 80 degrees Celsius for 15 minutes.
Optimum pH is between 2.5 and 5.0. Removal of Ca(2+) from the protein by EDTA treatment narrows the pH range to 2.5-3.5.
Optimum temperature is approximately 45 degrees Celsius. Removal of Ca(2+) from the protein by EDTA treatment decreases the optimum temperature to 35 degrees Celsius.
Optimum at neutral pH.
Optimum pH is 7.4 for the thioesterase activity.
Optimum pH is 7.0 (PubMed:10821871). Optimum pH is 8.5 (using glutaredoxin as electron donor) (PubMed:20059400).
Optimum temperature is about 37 degrees Celsius. Thermostable. Still fully active after heating at 80 degrees Celsius for 24 hours. Activity begins to decrease after heating at 98 degrees Celsius for 30 minutes. Inactive after heating at 110 degrees Celsius for 30 minutes.
Optimum pH is 8.0. Retains 70% of activity after 16 hours incubation in the pH range 4-11, at 37 degrees Celsius.
Optimum temperature is 60 degrees Celsius. Retains more than 90% and 70% of activity after 30 min incubation at pH 6 at 50 and 60 degrees Celsius, respectively.
Thermostable. Complete trypsin inhibition at all temperature ranges (25-100 degrees Celsius).
Optimum pH is 7.5. Highly active from pH 6 to 8.
Optimum temperature is 25-37 degrees Celsius. Thermolabile. Approximately 45% of the activity remains after incubation for 15 min at 45 degrees Celsius.
Optimum pH is 8.0-10.0.
Optimum pH is 7.5-8.5 for the forward reaction (PEP formation) and 6.0-7.5 for the reverse reaction (2-PGA formation).
Optimum pH is 6. Larger substrate spectrum at pH 7.5, with a lower activity, thought.
Optimum pH is about 8.5 for the oxidation of L-serine. Stable from pH 7.5 to 10.5.
Optimum pH is between 6.4 and 7.2.
Optimum pH is 12-13.
Optimum temperature is 85 degrees Celsius. Stable at 90 degrees Celsius.
Optimum pH is 7.5-7.8 for isoglutaminyl synthase activity.
Optimum pH is 6.5-7.0 for ergothioneine uptake and transport efficiency decreased at more alkaline pHs.
Optimum pH is 6.5 for the oxidation of D-glucose by NADP(+). At the physiological pH of 4.6 found in the cytoplasm of Picrophilus cells, the enzyme shows merely 10% of its maximal activity.
Optimum temperature is 55 degrees Celsius for the oxidation of D-glucose by NADP(+).
Optimum pH is 8.0. Active from pH 4.0 to 10.0. In unbuffered solutions, the dodecameric complex is active at pH 3.0.
Optimum pH is 7.0 for elastin hydrolysis.
Optimum temperature is between 70 and 80 degrees Celsius.
Optimum pH is 9.5 (in the presence of Mg(2+)). No activity at pH 7.5.
Optimum temperature is 45.50 degrees Celsius.
Optimum pH is 5.5. Active from 4 to 6.5.
Retains all activity when heated up to 90 degrees Celsius for 10 minutes and retained 38% activity after 10 minutes at 100 degrees Celsius.
Optimum pH is 7. Active between pH 6 and pH 8.
Optimum pH is 5.5. Inactive at neutral pH.
Optimum temperature is 85 degrees Celsius. At 95 degrees Celsius, the enzyme activity is still 66% of maximum, whereas at 40 degrees Celsius the enzyme is almost inactive. Thermostable.
Optimum pH is 7.5-8.4. Active from pH 6 to 8.5.
Optimum pH is 2.0.
Optimum pH is 6.8-7.3 in the reductive reaction and pH 8.3-8.6 in the oxidative reaction.
Optimum pH is 6.5 to 7.4.
Highly thermostable. Retains about 70% of its activity after heating for 60 minutes at 100 degrees Celsius.
Optimum pH is 9. Active from pH 7 to pH 11.
Optimum pH is around 8 and 7.6 for sarcosine and dimethylglycine, respectively. The pH optimum appears to depend on the buffer used.
Optimum pH is between 7.5 and 8.0.
Optimum pH is about 8.5 for the oxidation of L-serine. Stable from pH 6.5 to 10.0.
Still active after heating at 55 degrees Celsius for 80 minutes.
Optimum pH is 8.0. Retains nearly 60% enzyme activity at pH 6.0. The relative stability of purified enzyme is high at acidic pH and neutral pH (4.0-7.0) as compared to its relative stability at higher pH (9.0-10.0).
Optimum pH is 7.5. Exhibits K(+)/H(+) antiporter activity between pH 7.5 and 9.5.
Optimum pH is above 10.
Optimum pH is 7.9-8.5 for CTIL substrate.
Optimum temperature is 30-37 degrees Celsius for CTIL substrate.
Optimum temperature is about 80 degrees Celsius. MazG is very stable at high temperature, and no change is detected up to 85 degrees Celsius.
Optimum pH is 8.5 for the forward reaction, and 6.6-7.3 for the reverse reaction. The half-maximal rate is observed at 6.8 for the forward reaction, and at 5.5-7.9 for the reverse reaction.
Optimum pH is 8 with myricetin as substrate (in the presence of S-adenosylmethionine).
Optimum pH is 7.0-7.5 (PubMed:14519127). Optimum pH is 6.0-6.5 (PubMed:15598885). Active between pH 4.3 and 8.3. Stable after 12 hours incubation from pH 4.0 to 9.0.
Optimum temperature is 90 degrees Celsius. Is extremely thermostable.
Stable for 20 min at temperatures of up to 80 degrees Celsius.
Optimum pH is 8.0. No activity at pH 6.0.
Optimum pH is around 8.0 for n-butylamine oxidation.
Optimum pH is 1.7 with hemoglogin as substrate.
Optimum pH is 4.3. Stable between pH 3.0 and 11.0 in the presence of BSA, and pH 4.0 and 9.5 in the absence of BSA.
Loses 30% of activity after 10 minutes incubation at 50 degrees Celsius, activity is abolished after 10 minutes incubation at 60 degrees Celsius.
Optimum pH is 7.0. Active between pH 6.0-8.5.
Optimum temperature is 90 degrees Celsius for membrane-bound enzyme but 80 degrees Celsius for the purified enzyme. Membrane-bound enzyme has a half-life of 2h at 100 degrees Celsius whereas the half-life of the purified enzyme is 30 minutes at 100 degrees Celsius.
Optimum temperature is between 70-90 degrees Celsius. Highly thermostable. 34% of the activity of the unheated sample after incubation at 90 degrees Celsius for 10 hours. Lowest activity detected at 10 degrees Celsius.
Activity increases as temperature increases from 0 to 45 degrees Celsius and decreases markedly at temperatures greater than 55 degrees Celsius.
Optimum pH is 5.0-5.6 at 60 degrees Celsius.
Optimum pH is 8.5-9 for azetidine-2-carboxylate.
Retains more than 90% of its maximum activity between pH 5.4 and 6.4 in the ionic strength range of I=0.005-0.01. In buffers of I=0.005 more than 75% of maximum activity is retained between pH 5.3 and 7.5. In buffers of I=0.02 more than 75% of maximum activity is retained between pH 5.3 and 6.5.
Optimum temperature is 55 degrees Celsius. No decrease in activity was detected after incubation at 30 degrees Celsius for 30 minutes. No activity could be detected after incubation at 90 degrees Celsius for 30 minutes or 100 degrees Celsius for 10 minutes.
Optimum pH is 6.3 with more than 50% activity occurring between pH 5.2 and 7.9.
Enzymatic activity increases 12-fold in response to a change in temperature from 25 to 65 degrees Celsius. Displays a half life of 43 hours at 55 degrees Celsius.
Optimum pH for phosphorolysis of trehalose is 6.7. Stable between pH 6.0 and 7.0.
Optimum temperature for phosphorolysis of trehalose is 36 degrees Celsius. Stable below 30 degrees Celsius, activity decreases to 58% of maximum after 3 minutes incubation at 40 degrees Celsius.
Resistant to heat.
Optimum pH is at least 9.5, the highest pH value tested. No activity is observed below pH 8.5.
Optimum pH is 5.5 in both directions.
Optimum temperature is 60 degrees Celsius in both directions.
Optimum pH is 5.7-6.5.
Stable from pH 3.0 to 12.0.
Highly thermostable, when continuously exposed at the temperatures ranging from 20 to 100 degrees Celsius for 30 minutes.
Optimum temperature is 37 degrees Celsius. Retains 70% and 20% of activity when incubated at 42 degrees Celsius for 45 and 120 minutes, respectively. Activity is lost after 200 minutes incubation at 42 degrees Celsius.
Optimum temperature is below 40 degrees Celsius.
Optimum temperature is 50 degrees Celsius. Displays half maximal activity in 25 minutes and 7 minutes when pre-incubated at 50 degrees Celsius and 55 degrees Celsius, respectively.
Optimum temperature is 20 degrees Celsius (at low osmolality).
Optimum pH is 8.0 for UDP and 7.5 for GDP.
Optimum pH is 9 with ferricenium ion (Fc(+)) as electron acceptor. The enzyme is stable from pH 4 to pH 10.
Optimum temperature is 63 degrees Celsius with ferricenium ion (Fc(+)) as electron acceptor.
Active over the wide range of pH from 3 to 9.
Optimum pH is 7.5. Is stable in the pH range from 5.5 to 10.5.
Optimum temperature is 55 degrees Celsius. Is stable at temperatures up to 65 degrees Celsius.
Optimum pH is 10.0-10.5 for the oxidative deamination of L-leucine, L-isoleucine and L-valine.
Thermostable. Retains full activity on incubation for 10 min at 65 degrees Celsius, but loses about 75% of the activity at 75 degrees Celsius.
Optimum pH is 7.9. Active from pH 6 to 8.9.
Optimum pH is 7. Very little activity is detected at pH 6.1 or 8.0.
Optimum temperature is 55 degrees Celsius. Very little activity is found above 65 degrees Celsius, and the activity at 40 degrees Celsius is 50% of the maximum.
Optimum pH is approximately 7.8.
Optimum temperature is 37 degrees Celsius. Activity is rapidly lost after incubation for 30 minutes above 40 degrees Celsius.
Optimum pH is 8. Is essentially inactive at pH 9.0.
Optimum pH is 7.0. Activity is significantly retained from pH 6.0 to 9.0.
Activity is decreased at temperatures higher than 40 degrees Celsius.
Optimum temperature is 30 degrees Celsius. It loses above 50% decarboxylase activity at 40 degrees Celsius for 5 minutes, and above 25% enzyme activity remains after incubation at 50 degrees Celsius for 5 minutes.
Optimum pH is 7.5. Has activity up to pH 9.5.
Optimum pH is 7.2-9 with 2-arachidonoyglycerol as substrate.
Optimum pH is 8.5. It shows 50% activity at pH values of 6 and 10.
Optimum pH is 8.5-9 for azetidine-2-carboxylate and 6.5-7 for (S)-1-pyrroline-5-carboxylate.
Optimum pH is 6.5-7.5. Inactive at or below pH 5.0.
Optimum temperature is between 30-35 degrees Celsius.
Optimum temperature is 23 degrees Celsius.
Optimum pH is 6.5-7.5 for the reverse reaction.
Optimum pH is 6.5. Active in slightly acidic to neutral pH range.
Optimum pH is 9.0 for sorbitol oxidation and 7.5 for D-fructose reduction.
Optimum pH is 7.5. Stable in the range of pH 5.0-8.0.
Stable up to 35 degrees Celsius.
Optimum pH is between 6 and 7.
Activity remains fairly constant between 20-55 degrees Celsius. Active even at 0 degrees Celsius.
Optimum pH is 6 (PubMed:9108257, PubMed:11427234). A second pH optimum was found at pH 10 (PubMed:9108257, PubMed:11427234).
Optimum pH is 7.5 for N-dodecanoylsphing-4-enine hydrolysis.
Optimum pH is 7 (PubMed:21659511). Active between 5-8.5 (PubMed:21659511).
Optimum pH is 7-7.5 (PubMed:20591164, PubMed:33536500, PubMed:19196988). Active from pH 5 to 9 (PubMed:33536500, PubMed:19196988). Has less than 20% of normal activity at pH 6.0 (PubMed:19196988).
No loss of activity after three months at minus 35 degrees Celsius.
Optimum pH is 5.6 to 6.4.
Optimum pH is 5.25 for the catalase reaction. Stable from pH 5.5 to pH 11.
Optimum pH is 7.5-9.
Optimum pH is 8. Active in a broad range of pH varying from 6 to 9.
Optimum pH is 8 for both directions of the reaction.
No activity can be observed at or below 50 degrees Celsius.
Optimum pH is7.5-7.6.
Highly thermostable. Enzyme activity is maintained up to 45 degrees Celsius. Active at 50 degrees Celsius but with reduced catalytic activity.
Optimum pH is 6.5 for acetyl-CoA reductase and 7.5 for acetaldehyde dehydrogenase activities.
Optimum pH is 7.5. Active from pH 5.5 to 9.5. At pH 5.5, exhibits less than 15% of its optimal activity.
Optimum pH is 6. Active between pH 4-7.
Optimum pH is 9.2.
Optimum pH is between 6.5 and 7.5.
Optimum temperature is between 80 and 100 degrees Celsius. At 80 degrees Celsius, the dehydratase is stable for over 2 hours, but at 90 degrees Celsius its activity is decreased to less than 50% in 2 hours and its half-life diminishes to 120 min. At 100 degrees Celsius, the enzyme has a half-life of less than 40 min. The activity is almost undetectable below 60 degrees Celsius.
Optimum temperature is 37-50 degrees Celsius.
ATPase activity is not temperature compensated, unlike the circadian oscillator, its activity increases with increasing temperature.
Optimum pH is 7.2-8.0.
Optimum pH is 8.5-10.5.
Optimum pH is 3.5-6. Stable from pH 3.5 to pH 8.
Optimum temperature is 20-50 degrees Celsius.
Optimum pH is 5.5. Active from 2.5 to 7.5 with phytic acid as substrate. The optimum pH is shifted to more acidic values with 4-nitrophenyl phosphate as substrate.
Optimum pH is 8-8.5. Activities at pH 7 and pH 9.5 are 35% and 85% of the maximal activity, respectively.
Optimum pH is about 7.0. Active from pH 6 to 9.
Optimum pH is 7.7-8.2.
Optimum pH is 7.5. Active from pH 6.8 to 8.2.
Active between 20 and 55 degrees Celsius.
Optimum pH is 6.5 for catalase activity. Active from pH 4.5 to 8.5.
Is thermostable. Retains >95% of its activity after incubation at 60 degrees Celsius for 30 minutes.
Optimum temperature is 55-70 degrees Celsius for cleavage of gDNA:target RNA hybrids, increased cleavage and background hydrolysis are seen at 70 degrees Celsius (PubMed:19092929). gDNA:tDNA cleavage is enhanced at 75 degrees Celsius (PubMed:19812667). Activity on 98 nt tDNA occurs at 20 degrees Celsius, but on plasmid dsDNA only occurs at 65 degrees Celsius or higher (PubMed:24531762).
Optimum pH is 7-9 with 3-hydroxyisobutyryl-CoA as substrate and 6 with 3-hydroxypropionyl-CoA as substrate.
Activity is stable between pH 2 and 9 but decreases at a more basic pH.
Thermostable. Activity remains unaffected even after incubation at 100 degrees Celsius for 30 minutes.
Irreversibly unfolds upon heat treatment (PubMed:23601645). Between 50 and 65 degrees Celsius, the protein shows an intermediate fold (PubMed:23601645). At more than 65 degrees Celsius, the protein is completely unfolded (PubMed:23601645).
Active at low temperatures, even below 0 degree Celsius. Thermal denaturation midpoint (Tm) is 56.4 degrees Celsius.
Optimum pH is 6.5 with ONPG as substrate. Over 90% of activity in the pH range 6.5-8.5. Highly efficient at pH range 4.5-9.5.
Optimum temperature is 50 degrees Celsius with ONPG as substrate. Active at 4-8 degrees Celsius. 60% of the maximum activity is detected at 25 degrees Celsius and 15% at 0 degrees Celsius. Over 50% activity at 30 degrees Celsius.
Optimum pH is 6.0 for hydrolysis of alpha-cyclodextrin.
Optimum temperature is 55 degrees Celsius for hydrolysis of alpha-cyclodextrin. Thermostable. No significant loss of catalytic activity even after overnight incubation at 65 degrees. EDTA enhances the thermostability, the half-life being 90 min at 70 degrees Celsius in the absence of EDTA, increasing to 360 min in the presence of 10 mM EDTA. However, overnight incubation at 4 degrees Celsius is first required for enhancement of thermostability. Activity decreases drastically at 75 degrees Celsius.
Thermostable. Retains virtually all of its activity after incubation at 100 degress Celsius for 3 hours.
Activity is stable between pH 2 and 11.
Activity is stable up to 60 degrees Celsius and declines rapidly at higher temperatures.
Optimum pH is 6.5-7.5 for both the catalase and the peroxidase reaction.
Optimum pH is 7. The enzyme is active between pH 5.0 and 9.0.
Optimum pH is 8.2 (PubMed:16849334, PubMed:17610898). Is more than 50% active in a relatively narrow pH range from 7.3 to 9.3 (PubMed:17610898).
Optimum temperature is 91 degrees Celsius.
Highly thermostable, with a Tm value of 70 degrees Celsius. Incubation at 60 degrees Celsius for two hours has no apparent effect on KLK7 inhibition activity. Polymerization is observed at 70 degrees Celsius and above.
Optimum pH is around 8. The enzyme is stable under neutral and alkaline conditions, while its activity decreases rapidly below pH 6.0.
Optimum temperature is 37 degrees Celsius. It lost its activity rapidly below 25 degrees Celsius or above 45 degrees Celsius.
Optimum pH is 8.0. Active from pH 6.0 to 9.0.
Optimum pH to bind cytokinin is about 7-8.5 at 0 degrees Celsius.
Cytokinin-binding is more stable at 0 degrees Celsius than at 20 and 37 degrees Celsius.
Optimum pH is 7.0-10.5.
Optimum temperature may be above 100 degrees Celsius. Activity observed at 100 degrees Celsius is about 8 times that at 37 degrees Celsius. Thermostable up to 95 degrees Celsius. Retains full activity after heating at 90 degrees Celsius for 10 min and more than 95% of the full activity at 100 degrees Celsius for 10 min.
Optimum pH is 8.1 at 22 degrees Celsius with Suc-AAA-pNA as the substrate.
Optimum pH is 10. Is stable for 1 hour at 40 degrees Celsius in the pH range 4.0-8.0.
Optimum temperature is 50-55 degrees Celsius. More than 50% of maximum activity is found in the temperature range 40-60 degrees Celsius. Retains more than 50% of the initial activity after 4 hours incubation at 40 degrees Celsius at pH 10, while at 50 degrees Celsius only 1% of the initial activity is found after the same treatment.
Optimum pH is 8.0. It is highly thermosatble.
Hemagglutinating activity is stable over the pH range 4.0-12.0 but decreases below pH 3.
Hemagglutinating activity is stable when incubated over the range of 20-70 degrees Celsius for 60 minutes but is almost lost after incubation for 60 minutes at 80 degrees Celsius.
Optimum pH is 3.5 with alpha-naphthyl phosphate as substrate.
Optimum pH is 5 (food vacuole) (PubMed:12876284). Optimum pH is 7 (PubMed:12876284).
Optimum pH is 5.0 for the decarboxylation of L-tyrosine. Is not active at pH below 3.5 or above 9.0. More than 90% of activity remains over pH range from 5.0 to 6.0, and activity decreases rapidly at pH below 5.0 or higher than 6.0.
Optimum temperature is 50 degrees Celsius. Only 8.5% of activity remains at 70 degrees Celsius. However, the enzyme shows poor stability at 50 degrees Celsius, the relative activity dropped to 14% after 1 h of incubation.
Optimum pH is 6.25-6.75.
Optimum pH is 9.0 for the hydrolysis of Gly-Arg-pNA. No hydrolysis of Gly-Arg-pNA is detected below pH 5.5 or above pH 11.5. Stable over a broad pH range of between 7.5 and 10.0.
Optimum temperature is between 35 and 40 degrees Celsius for the hydrolysis of Gly-Arg-pNA. Stable for at least 30 minutes below 20 degrees Celsius.
Optimum temperature is 67 degrees Celsius.
Optimum pH is 5.5. Another high activity level is observed at pH 9.4.
Stable up to about 62 degrees Celsius.
Activity is lost at pH 6.
Optimum pH is 7.5 to 8.5.
Optimum temperature is above 30 degrees Celsius. Heating to 60 degrees Celsius for 1 minute abolishes 72% of the original mutase activity, and no activity remains after 8 minutes.
Optimum temperature is 45 degrees Celsius. Thermal denaturation midpoint (Tm) is 47.6 degrees Celsius and is raised to 66.0 degrees Celsius when AK is complexed with the inhibitor Ap5A.
Optimum pH is around 11.0 for oxidative deamination (PubMed:24835098). Optimum pH is 7.5 for reductive amination (PubMed:24835098). Retains more than 80% of its activity after incubation for 30 min at pH 5.0-11.5 (PubMed:24835098).
Optimum temperature is 45 degrees Celsius for oxidative deamination at pH 11.0 (PubMed:24835098). No enzyme activity is lost after incubation for 10 min at temperatures up to degrees 40 Celsius, but shows complete loss of activity at temperatures above 50 degrees Celsius (PubMed:24835098).
Optimum pH is 4.5 with LNB-beta-pNP as substrate and pH 6.0 with PA-lacto-N-tetraose.
Optimum pH is 8. Less active under acidic conditions.
Thermostable. Retains about 80% of its activity after treatment of 65 degrees Celsius for 30 minutes.
Optimum pH is 7.5 for choline dehydrogenase and is between 7.5 and 9.5 for glycine betaine-aldehyde dehydrogenase.
Optimum temperature 37 degrees Celsius. Thermostable. Retains 93, 78 and 15 % of its protein acetyltransferase activity after heating to 100 degrees Celsius for 5, 30 and 60 minutes, respectively.
Optimum pH is 8. Highly stable from pH 6.5 to 9.0, and still shows a residual activity of 50% after 1 hour of incubation at pH 4.0.
Stable for at least 12 hours at 25 or 37 degrees Celsius (at pH 7.6).
Optimum pH is 6.7 with 10 mM putrescine and 10 mM carbamoyl phosphate, 7.8 with 0.2 mM putrescine and 0.2 mM carbamoyl phosphate, and 9.0 with 0.5 mM putrescine and 10 mM carbamoyl phosphate.
Optimum temperature is around 60 degrees Celsius.
Inactivated by mild heat treatment (42 degrees Celsius during 10 minutes).
Wide extracellular pH range, between 4.0 and 9.0.
Optimum pH is 6-10.
Optimum pH is 9.0 for reductive amination of pyruvate and pH 9.0-11.5 for oxidative deamination of alanine.
Optimum temperature is 55-60 degrees Celsius for reductive amination and 50-55 degrees Celsius for oxidative deamination. But at 60 degrees Celsius an inactivation of the enzyme is observed after about 1 minute, while at 65 degrees Celsius the enzyme is inactive after about 10 seconds.
Optimum pH is 7.0 for the reduction of rubredoxin.
Optimum temperature is 37 degrees Celsius. It has about 60% activity at 30 degrees Celsius, the optimal growth temperature for this bacteria.
Optimum pH is 9.4-9.8.
Optimum pH is 5.3. Retains over 90% of its activity in the pH range of 5 to 6.
Optimum pH is 6.5 with prostaglandin E2 and L-thyroxine (T4) as substrates.
Optimum temperature for ATPase activity is 80 degrees Celsius.
Not very thermostable.
Optimum pH is 7-7.4 with 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate as substrate.
Optimum pH is 7.6-7.8.
Optimum pH is 8.0-9.0 for sodium transport and 8.0 for lithium transport.
Optimum pH is 4.5-9.0.
Optimum pH is 5.8-6.2.
Optimum pH is 8.0-8.5. Active from pH 6 to 8.5.
Optimum pH is 5 with N-palmitoylethanolamine as substrate.
Trypsin inhibitory activity is stable after incubation at pH 2 to pH 10 at 60 degrees Celsius for 1 hour.
Trypsin inhibitory activity is stable after incubation at 90 degrees Celsius for up to 2 hours.
Optimum pH is 7.8-8.4 for both catalytic activities.
Optimum pH is 6.8. At 70 degrees Celsius for 60 minutes, the enzyme does not lose activity in a pH range of 4.0 to 10.5.
Extremely high thermostability. Upon heating at 95 degrees Celsius for 10 minutes the kinase activity is not lost, but about 80% of the activity is lost upon incubation at 100 degrees Celsius for 10 minutes.
Optimum pH is between 6.5 and 7.5. At pH 5.5, EntB retains 50% of isochorismatase activity.
Optimum pH is 6.5 for DGPP phosphatase activity and 6.5-7.5 for undecaprenyl pyrophosphate phosphatase activity.
Optimum temperature is 31 degrees Celsius. Cold-active. Is rapidly inactivated at 45 degrees Celsius, and shows significant activity at 10 degrees Celsius and below.
Optimum pH is 9.35.
Optimum pH is 4.6-9.6.
Optimum pH is 7.0-8-0.
Optimum pH is 3.5 with Reactive Blue 19 as substrate.
Optimum temperature is about 25 degrees Celsius. Thermostable. Retains 50% of its activity after heating 2 hours at 60 degrees Celsius, while no decrease in activity is observed within the same time at 30 or 40 degrees Celsius.
Thermostable at 100 degrees Celsius for 15 minutes, but loses activity at the same temperature within 30 minutes.
Optimum temperature is 49-54 degrees Celsius.
Optimum pH is between 5 and 5.5.
Optimum pH is 8.0 for the isomerization of androst-5-ene-3,17-dione.
Thermostable up to 80 degrees Celsius.
Is hyperthermostable. Displays a Tm value of 84.9 degrees Celsius, which increases to 96.2 degrees Celsius upon biotin binding.
Optimum pH is 9.5 in the direction of L to D, and 10 for the reverse direction.
Optimum temperature is 37 degrees Celsius for both directions.
Optimum pH is 6.0, in the intact toxin complex optimum pH is pH 4.0 to pH 8.0.
Optimum pH is 7.0-8.0. for arylsulfatase activity.
Optimum pH is 7.5. Active in a broad range pH.
Optimum pH is 7.5 with one-half of the maximal activity at pH 6 and 9.5.
Optimum temperature is 90 degrees Celsius. No loss of activity is observable even after exposure of the purified enzyme to 100 degrees Celsius for 1 hour. At 60 and 110 degrees Celsius the reaction rate is one-half of the maximal value, and a residual 20% activity is still observable at 120 degrees Celsius.
Optimum temperature is about 55 degrees Celsius.
Optimum temperature is above 95 degrees Celsius. Extremely thermostable.
Optimum pH is 7.0 for decarboxylation.
Optimum temperature is 60 degrees Celsius for decarboxylation. Optimum temperature is 45 degrees Celsius for carboxylation.
Active from 25 to 80 degrees Celsius.
Optimum pH is 9.5. Active from pH 8 to pH 10.5.
Optimum pH for hemagglutinating activity is between pH 5 and pH 7. Activity quickly decreases outside this range.
Hemagglutinating activity is stable up to 50 degrees Celsius and is lost after incubation for 1 hour at 80 degrees Celsius.
Optimum pH is 8.0. Shows more than 60% of its maximum activity in the pH range of 7.0-9.5. 20% of the activity is retained at low pH range and at pH 10.5, while no catalytic activity is found at pH 5.0 or 10.5.
Optimum temperature is 35 degrees Celsius with p-nitrophenyl acetate as substrate. Above 35 degrees Celsius, activity significantly decreases, while 30% of the maximum activity is recorded at 10 degrees Celsius.
Optimum temperature is 65 degrees Celsius. Protected from thermal inactivation by ATP.
Highly stable at high temperatures.
Thermostable. The formation of the dimer of dimers is important to increase the thermostability.
Optimum pH is 8.0 in cell-free extracts. Activity decreases as pH is raised or lowered.
Optimum temperature is 55 degrees Celsius (PubMed:23604968). Optimum temperature is 65 degrees Celsius (PubMed:32269349).
Optimum temperature is 106 degrees Celsius at pH 7. Highly thermostable.
Optimum pH is 5.0 for the forward reaction, and 6.0 for the reverse reaction.
Optimum temperature is 50 degrees Celsius for the forward reaction, and 45 degrees Celsius for the reverse reaction.
Complexation with ADP increases the thermal unfolding temperature from 40 to 65 degrees Celsius.
Optimum pH is 8.5 and 10 (with pyruvate as cosubstrate).
Optimum temperature is 60 degrees Celsius (at pH 8.5 and with pyruvate as cosubstrate).
Denaturates after heat treatment at 100 degrees Celsius for 10 min. The N-terminal region (18-198) is thermostable and retains its ability to bind to curdlan after heat treatment at 100 degrees Celsius for 10 min. The C-terminal region (199-488) is heat-labile after the same treatment.
Optimum pH is 9.0 (with pNP-acetate as substrate).
Optimum temperature is 45 degrees Celsius (with pNP-acetate as substrate).
Methyltransferase activity is much more higher at 65 degrees Celsius than at 55 and 37 degrees Celsius.
Optimum temperature is 16 to 42 degrees Celsius.
Optimum pH is 7.6 with 3-oxooctanoyl-CoA and 3-oxo-2-methylpalmitoyl-CoA as substrates (PubMed:9325339). Optimum pH is 9.5 with 3alpha,7alpha,12alpha-trihydroxy-24-oxo-5beta-cholestan-26-oyl-CoA as substrate (PubMed:10706581).
Stable at low pH. Refolds at pH 2.5 after thermal denaturation. Thermal denaturation is irreversible at pH 7.5.
Resistant to heat (PubMed:26417906, PubMed:11898007). At room temperature no effect in protein secondary structure by varying pH (PubMed:26417906).
Thermostable between 4 and 25 degrees Celsius. At -20 degrees Celsius, the remaining activity is 20%. Heating at 70 degrees Celsius inactivates the enzyme in 15 minutes.
Optimum pH is 7.5. Active from pH 6 to 10.
Optimum pH is 6.7-7.5 (at 30 degrees Celsius).
Optimum pH is 7.0-7.5 (isoform 1). Optimum pH is 8.5 (isoform 2).
Optimum pH is 6 and 6.5 for the synthetic and phosphorolytic reaction, respectively. Thermostable. Has a half-life of 60 hours at 60 degrees Celsius.
Optimum pH is 9 at high salt concentrations.
Optimum pH is 7 for L-rhamnose transport.
Optimum pH is 6.5-7.0 for both catalytic activities.
Optimum temperature is 40 degrees Celsius for both catalytic activities.
Optimum temperature is 90-96 degrees Celsius. Highly thermostable.
Optimum pH is 6.5 (at 55 degrees Celsius).
Extreme thermal stability. It maintains about 50% of its activity after heat treatment for 60 min at 100 degrees Celsius.
Thermostable. No loss of IMPase activity after incubation at 85 degrees Celsius for 30 minutes. Heating at 100 degrees Celsius for 10 minutes results in a loss of about 20 to 25% of the activity, and 30 minutes at this temperature inactivate about 70% of the activity.
Is most active at neutral pH.
Optimum pH is 7.5 (PubMed:10700397, PubMed:20652169). Optimum pH is 7.8 for acetylserotonin O-methyltransferase activity (PubMed:25039887).
Optimum pH is 2.9-3.3.
Optimum pH is between 7.5 and 8. The mutase activity decreases sharply at pH 6.5 and 9 to about 20% of that observed at the optimum pH.
Optimum temperature is 37 degrees Celsius. At 60 degrees Celsius the mutase activity is completely lost.
Optimum temperature is 40 degrees Celsius. At higher temperatures, the activity decreases rapidly. Shows high enzyme activity with casein as a substrate under the storage conditions of UHT milk. Is highly thermostable. Withstands general ultra-high temperature (UHT) processing (138 degrees Celsius for 18 seconds) in skim milk, with 88% of the initial enzyme activity remaining after heating. The milk matrix has a protective effect on the enzyme activity during the thermal process.
Optimum pH is about 7.5. The reaction rates at the pH values of 6.8 and 8.0 are about 60% of the pH 7.5 rate.
Optimum temperature is 55 degrees Celsius. The half-maximal activity is reached at 26 degrees Celsius and 78 degrees Celsius.
Optimum pH is 5.0-6.5.
Optimum pH is 8.5 with ferricenium ion (Fc(+)) and 7.0 with 1,4-benzoquinone as electron acceptor, respectively.
Optimum temperature is 80 degrees Celsius, 90% of activity remains after heating at 70 degrees Celsius for 5 minutes.
Optimum temperature is 80 degrees Celsius for caseinolytic activity.
Optimum temperature is 29.5 degrees Celsius.
Optimum temperature is 46-50 degrees Celsius.
Optimum pH is around 9.0.
Inactive at 80 degrees Celsius.
Optimum temperature is 50 degrees Celsius. Loses activity when is heated at 55 degrees Celsius.
Optimum pH is 6.5-7.0. The half-maximal rate is observed at 5.2 and 8.1.
Optimum pH is between 7.0 and 8.5.
Optimum pH is 3.0. Stable under acidic conditions. Retains over 70% of its original activity at pH range of 3-6.
Optimum pH is 6.5 with glucose-pentaacetate as substrate. Active from pH 5.0 to 8.0.
Optimum temperature is 35 degrees Celsius with glucose-pentaacetate as substrate. Active from 20 to 50 degrees Celsius. Still exhibits 40 to 70% of the maximum activity after 20 hours of incubation at 50 degrees Celsius.
Optimum pH is 3.8 with 2.2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, 5.5 with ferulic acid as substrate, and 6.5 with N-acetyl-L-tyrosine as substrate.
Loss of activity at temperature above 55 degrees Celsius. In the presence of excess calcium, full activity is kept at 65 degrees Celsius for 90 minutes.
Optimum pH is about 9.8 for the oxidative deamination of meso-diaminopimelate and 7.9 for the reductive amination of L-2-amino-6-oxopimelate.
Optimum temperature is above 98 degrees Celsius.
Optimum pH is 8.0-8.4.
Optimum temperature is 90-95 degrees Celsius. Highly thermostable.
Optimum pH is 10.5 for the L-fucokinase activity and 6.5-8 for the GDP-fucose pyrophosphorylase activity.
Optimum temperature is 40 degrees Celsius for the L-fucokinase activity and 30-45 degrees Celsius for the GDP-fucose pyrophosphorylase activity.
Optimum pH is 8.73 for transfer of p-nitroanilide from gamma-GpNA to gly-gly and 9.25 for hydrolysis of gamma-GpNA.
Optimum temperature is 50 degrees Celsius for both transferase and hydrolase activities.
Thermostable at 90 degrees Celsius.
Optimum pH is 8.5. Shows high stability over a broad range of pH 4-9.5 (80-100% of enzyme activity). Becomes unstable at pH over 10, where the activity drops to 34% at pH 10.5 and to 8% at pH 11.
Optimum temperature is 20-30 degrees Celsius (DOI=10.1016/j.enzmictec.2008.01.009). The His-tagged protein shows a higher optimum temperature of 55 degrees Celsius, and a thermal denaturation midpoint (Tm) of 66.63 degrees Celsius (PubMed:19041687). Is stable when incubated for 30 minutes at temperatures in the range of 30-70 degrees Celsius at pH 7 (DOI=10.1016/j.enzmictec.2008.01.009).
Retains almost half of its activity after treatment at pH 2.0 for 3 hours at 20 degrees Celsius.
Inactivated after 3 minutes at 60 degrees Celsius or 1 minute at 80 degrees Celsius.
Optimum pH is between 7 and 8.2 for monooxygenase activity and between 5.5 and 6.5 for farnesene synthase activity.
Optimum pH is 6 and 6.5 in phosphorolytic and synthetic directions, respectively.
Optimum temperature is 42 degrees Celsius. Retains full activity after incubating at up to 55 degrees Celsius for 10 minutes, but only 35% is left after incubating at 60 degrees Celsius.
Thermostable. Still active after heating at 65 degrees Celsius for 15 minures.
Optimum pH is 8.0 for 2'-5' oligoadenylate exonuclease activity.
Optimum temperature is 75 degrees Celsius. Activity levels at 37 and 100 degrees Celsius are 20% of that observed at the optimal temperature.
Optimum pH is 7.5 (with 3'(2')-phosphoadenosine 5'-phosphate (PAP) as substrate).
Optimum temperature is 60 degrees Celsius (with 3'(2')-phosphoadenosine 5'-phosphate (PAP) as substrate) (PubMed:25372691). Active between 30 and 80 degrees Celsius (PubMed:25372691).
Optimum pH is 6.5-7.0. Active from pH 6.1 to 9.0.
Optimum temperature is 37-42 degrees Celsius. Active from 4 to 55 degrees Celsius. Inactive above 60 degrees Celsius.
Optimum pH is 6.8-9.8.
Optimum temperature is 62 degrees Celsius.
Optimum pH is 8.8 for triacylglycerol lipase activity, 8.5 for phospholipase activity and 8 for galactolipase activity. The enzyme activities decrease in the pH 5-7 range corresponding to the physiological conditions occurring in the small intestine.
Optimum pH is 5.6-7.6.
After incubation at 45 degrees Celsius for 30 minutes RpiA retains 90% of its original activities.
Optimum pH is 5.5 to 8.5.
Inactive below pH 7.0.
Optimum pH is 7.2, with half-maximal activities at pH 6 and 8.
Optimum pH around 9.
Optimum pH is 9 with testosterone and estradiol as substrates and 5.5 with androstenedione and estrone as substrates.
Activity increases with increasing temperatures between 4 and 42 degrees Celsius. Activity is below 20% of the maximum after exposure at 37 degrees Celsius for 30 minutes.
Optimum pH is about 4.
Optimum temperature is 60 degrees Celsius. Active between 20 and 80 degrees Celsius. Half-life at 60 degrees Celsius is 309 hours. Half-life at 70 degrees Celsius is 4.1 hours.
Optimum temperature is 75-80 degrees Celsius.
Optimum pH is 8 to 9.
Optimum pH is 10.0. Half maximum activity remains at pH 9.0 and 11.5. Stable from pH 6.5 to 11.0, activity is lost following 10 minutes incubation at pH 4.5 or 12.0.
Optimum temperature is 60 degrees Celsius. Retains 60% of maximum activity at 30 degrees Celsius and 65 degrees Celsius. After 15 minutes preincubation at 60 degrees Celsius 70% of the initial activity remains, 15 minutes preincubation at 65 degrees Celsius results in a total loss of activity.
Relatively stable at broad pH range (pH 1-11). Dimers are formed in strongly acidic (pH 2-5) and alkaline (pH 10-11) conditions.
Stable at wide temperature range (4-100 degrees Celsius). Heating does not cause degradation, but leads to polymerization. The polymer content is highest at around 70 degrees Celsius.
Optimum pH is 12. Is active over a wide pH range from 6.5 to 12.5.
Optimum temperature is 90 degrees Celsius. Extremely thermostable. The half-life of the enzyme is more than 2 hours at 85 degrees Celsius and 24 minutes in boiling water.
Optimum pH is 7-9.4.
Optimum pH is between 7.5 and 7.8.
Optimum pH is 8-11.
Optimal pH is about 5.7.
Optimum temperature is between 30 and 37 degrees Celsius.
Optimum temperatures are ranging from 40 to 50 degrees Celsius.
Optimum pH is 7.5 with (1,2-dilinoleoyl)-phosphatidylcholine as substrate.
Optimum pH is 5.5, also active at pH 7.0 with CUX1 as substrate.
Optimum pH is 8.5-7.5.
Optimum pH is 8 with vanillate as substrate.
Optimum temperature is 30 degrees Celsius with vanillate as substrate.
Active from 30 to 40 degrees Celsius.
Optimum pH is 6.8-7.3.
Optimum pH is 7.0-9.3 for the Suc-LLVY-Amc hydrolyzing activity (with the alpha1-beta proteasome subtype).
Optimum temperature is 75 degrees Celsius for the Suc-LLVY-Amc hydrolyzing activity (with the alpha1-beta proteasome subtype).
Optimum pH is between 6.0-6.2 for the amylase activity and 7.0 for the trehalose synthase activity.
Optimum pH is 5.0. Active from pH 2.0-7.0.
Stable at temperatures of up to 60 degrees Celsius.
Optimum pH is between 7.5 and 8.5.
Optimum pH is around 7.0 and 7.5 for the removal of selenium and sulfur atoms from L-selenocysteine and L-cysteine, respectively.
Optimum pH is 8.0. Half maximum activity is seen at pH 6.5 and 9.8.
Optimum temperature is 53 degrees Celsius.
Optimum pH is 6.8 at 50 degrees Celsius and 8.5 at 30 degrees Celsius.
Inactive at pH 2.0, activity is restored after 10 minutes incubation at pH 8.0.
Optimum pH is 8.3-9.6.
Optimum pH is 6.0-6.5 for the phosphorolysis of sucrose.
Optimum temperature is 48 degrees Celsius for the phosphorolysis of sucrose (PubMed:14740189). The His-tagged enzyme shows an optimum temperature of 58 degrees Celsius (PubMed:20691225). The immobilization of the enzyme on Sepabeads EC-HFA increases thermostability, and optimum temperature is shifted to 65 degrees Celsius (PubMed:20691225).
Optimum pH is around 5 (PubMed:15494009, PubMed:17302431, PubMed:12723619). More stable at alkaline than acidic pH (PubMed:15494009, PubMed:12723619, PubMed:17509134).
Stable below 45 degrees Celsius.
Optimum pH is 8.0 for FBP phosphatase activity and 7-8.5 for the FBP aldolase activity.
Highly thermostable. Survives boiling for 1 hour.
Highly active above 45 degrees Celsius. Poorly active at 25 degrees Celsius.
Optimum pH is 8.0 for 9,10-phenanthrenequinone-reduction. Optimum pH is 6.5-7.0 for azodicarboxylic acid bis[dimethylamide]-reduction (PubMed:10848984). Optimum pH is 6.0 with (2E)-hexenal or 4-hydroxy-(2E)-nonenal as substrate (PubMed:12514241).
Retains 65% activity after treatment at 65 degrees Celsius for 20 minutes.
Optimum pH is between 7.3 and 7.6 (PubMed:4893578, PubMed:4920894). The enzyme is stable between pH 4 and 9 (PubMed:4920894).
Stable in the pH range 2.2-10.0.
Stable at temperatures up to 70 degrees Celsius. Incubation at temperatures above 70 degrees Celsius steadily decreases inhibitory activity.
Thermostable. Optimum temperature is 25 degrees Celsius. The ability to inhibit trypsin slowly decreases as the temperature increases to 7% inhibition at 100 degrees Celsius.
Optimum pH is 5.8. Activity decreases at higher pH values.
Optimum pH for hemagglutinating activity is 7.4.
Retains hemagglutinating activity up to 42 degrees Celsius. The activity then drops and is completely lost at 65 degrees Celsius.
Optimum pH is 8.5. Activity is null at pH 7.0.
Shows more potent antibacterial activity at pH 6.2 (approximate coral mucus pH) than at pH 8.0 (approximate pH of seawater).
Stable from pH 5.5-8.5.
Thermostable, activity is retained after incubation at 90 degrees Celsius for 15 minutes.
Optimum pH is 7.4-8.8.
Thermostable for 60 minutes up to 55 degrees Celsius.
The melting temperature (Tm) is 65 degrees Celsius.
Optimum temperature is 54-55 degrees Celsius.
Unstable only at extreme acidic (pH 1.0) or alkaline conditions (pH 11.0). IgE-binding activity is relatively stable under acidic and alkaline conditions, however the activity is increased between pH 2.0-3.0.
Stable up to 100 degrees Celsius. IgE-binding activity is reduced with increasing temperature higher than 60 degrees Celsius.
Optimum pH is 6-7.5 and pH 6.5-8 for papain and bovine cathepsin B, respectively.
Stable between 20 and 40 degrees Celsius. Activity for papain drops rapidly over 60 degrees Celsius. Retains inhibitory activity against papain for 10 days at 37 and 30 degrees Celsius.
Optimum pH is 5-9.
Cytolytic activity upon C2C12 cells is completely abolished at 15 degrees Celsius or below.
Optimum pH is 6. Active and stable from pH 3 to 9.
Optimum temperature is 30-50 degrees Celsius. Active and thermostable for 30 minutes up to 55 degrees Celsius.
Broad temperature optima between 45 and 55 degrees Celsius. Reaction rate increases steeply up to 55 degrees Celsius. 50% of activity lost after incubation for 20 minutes at 57 degrees Celsius. Thermal stability increases in the presence of glycerol.
Optimum pH is 7.8 (Cc-LAAOI) and 7 (Cc-LAAOII).
Optimum temperature is 50 (Cc-LAAOI) and 60 (Cc-LAAOII) degrees Celsius.
Optimum pH is 8.0. Active over a broad pH range of 6.5-9.0.
Stable to heating at 50 degrees Celsius for 10 minutes. Rapidly inactivated by heating at 60 degrees Celsius.
Optimum pH is 5.5 with p-nitrophenyl maltoheptaoside or amylopectin as substrate.
Optimum pH is 7-7.5 at 35 degrees Celsius.
Optimum pH is 6.2-6.8.
Optimum temperature is 41 degrees Celsius.
Optimum pH is 5 with RE-(EDANS)-SQNYPIVQK-(DABCYL)-R as substrate.
Optimum pH is 7.3-7.9.
Does not demonstrate significant pH dependence in the pH range of 6.5-9.0.
Optimum pH is about 8.9 with D-alanine as substrate and about 9 with 3,4-dehydro-D-proline as substrate.
Active at 37 degrees Celsius. Retains activity after heating at 100 degrees Celsius.
Optimum pH is 3.4. Stability declines sharply below pH 2.8 and above pH 5.0.
Optimum temperature is below 30 degrees Celsius. Is very stable below 35 degrees Celsius, but at 50 degrees Celsius, it loses 80 percent of its activity within 2 h.
Optimum temperature is 0-10 degrees Celsius in the presence of 0.05 M NaCl, 15 degrees Celsius in the presence of 0.77 M NaCl, and 30 degrees Celsius in the presence of 3.0 M NaCl.
Highly thermostable. Has a melting temperature of 78 degrees Celsius at pH 7.4.
Optimum pH is 5.5 (in the presence of sodium acetate). Active between pH 6.5 and 9.
Optimum temperature is 50 degrees Celsius. Stable at 4 degrees Celsius at pH 6.5 - 9. 60% loss of activity when incubated at 45 degrees Celsius and pH 7.0 for 10 minutes.
Optimum pH is 8-9. Shows almost no activity at pH 5.
Optimum temperature for all combinations of guide:target is 60-65 degrees Celsius, the RNA product is more stable at 60 than 65 degrees Celsius.
Stable at acidic (pH 1.0-5.0) and alkaline (pH 9.0 and 11.0) conditions.
Thermostable even up to 100 degrees Celsius.
Optimum pH is 7.5 to 9.0.
Optimum pH is 7.5-9.5 for N-hexadecanoylsphing-4-enine (PubMed:10781606). Optimum pH is 7.5 for D-erythro-C12-NBD-ceramide (PubMed:16229686). Optimum pH is 7.5 for N-octanoylsphing-4-enine (PubMed:17475390).
Optimum pH is 5-6 with 1,3-DAG as substrate and at 30 degrees Celsius.
Optimum pH is 8-9, inactive at pH 6 and below.
Optimum temperature is 37-39 degrees Celsius.
Optimum temperature is 37-42 degrees Celsius.
Optimum pH is 7.5-8.5 for tyrosine decarboxylase activity.
Thermostable. Retains full tyrosine decarboxylase activity after heating at 100 degrees Celsius for 10 minutes and 42% of its activity after 10 minutes at 110 degrees Celsius. Inactive after 10 minutes at 121 degrees Celsius.
Pgm-A is more thermostable than Pgm-B.
Optimum pH is 6.5 with beta-cyclodextrin as substrate.
Optimum temperature is 85 degrees Celsius with beta-cyclodextrin as substrate. Retains 80% of its activity at 90 degrees Celsius. The half-lives of the enzyme examined at 80, 85, and 90 degrees Celsius are 147, 108, and 23 minutes, respectively.
Optimum temperature is 105 degrees Celsius.
Optimum pH is 8.5. Active between pH 4.5 and 9.5, stable between pH 6.5 and 10.5.
Optimum temperature is 45 degrees Celsius. Stable up to 40 degrees Celsius.
Stable over a wide pH range.
Optimum pH is about 3.8.
Optimum pH is 6.5-9.5.
Optimum temperature is 20-25 degrees Celsius.
Optimum pH is 9.5-10.0 for malate dehydrogenation, and 7.5-8.0 for oxaloacetate reduction.
Optimum pH is 3.6.
Optimum pH is 8.5 with 4-nitrophenyl phosphate as substrate. Has 50% residual activity at pH 7.5 and 30% at pH 9.0, being virtually inactive at pH 10 and at pH 5 or lower.
Heat-labile. Incubation for 10 minutes at 50 or 60 degrees Celsius causes 40% and 60% inactivation, respectively. Heating at 100 degrees Celsius for 2 minutes causes total loss of activity. Heat-inactivated enzyme cannot be renaturated.
Optimum temperature is below 30 degrees Celsius (PubMed:19810726). Optimum temperature is 30-40 degrees Celsius (PubMed:18658138).
Optimum pH is 6.7. Stable between pH 6.5 and 11.2.
Optimum temperature is under 35 degrees Celsius.
Optimum temperature is 25 degrees Celsius. Loses 70% of activity upon activation at 45 degrees Celsius for 15 minutes.
Optimum pH is 3.87-7.
Has a half life of 15 minutes at 60 degrees Celsius.
Optimum temperature is 65-70 degrees Celsius. Thermostable.
Optimum pH is 6, and the enzyme tends to precipitate gradually below pH 6.5.
Optimum pH is 8.0. Little or no activity below pH 4.2.
Retains over 80% of maximum activity at 15 to 60 degrees Celsius. Activity is greatly reduced over 70 degrees Celsius.
Optimum pH is 3.5. Unstable above pH 6.0.
Optimum pH is around 7.5.
Optimum temperature is 20-25 degrees Celsius. Loses activity at temperatures above 35 degrees Celsius.
Optimum pH is 5.95.
Optimum pH is 6.0. Retains over 95 percent activity in the pH range from 5.0 to 7.0, and 70 percent activity in the pH range from 4.0 to 8.0.
Optimum temperature is 58 degrees Celsius. Has complete stability at 60 degrees Celsius.
Optimum pH is 8.9. Active from pH 8.5 to 9.4.
Optimum pH is 6.4-6.8 with 4-hydroxyphenylacetamide as substrate.
Optimum temperature is 25 degrees Celsius with 4-hydroxyphenylacetamide as substrate.
Optimum pH is 4.25 for the peroxidase reaction and 7.5 for the catalase reaction.
Optimum temperature is around 60-70 degrees Celsius. Thermostable. Retains 100% activity for one week at 50 degrees Celsius and for 3 days at 65 degrees Celsius. Activity decreases rapidly at 75 degrees Celsius (half-life is about 15 minutes), and at 85 degrees Celsius loses its activity immediately.
Thermostable. Retains full catalytic activity after 9 hours at 55 degrees Celsius, and at 75 degrees Celsius the half-life is approximately 3 hours.
Optimum pH is around 6.
Stable up to 84 degrees Celsius.
Optimum pH is around 8.8. Activity drops sharply above and below the optimum and is absent at pH 3 and virtually so at pH 10.6.
Optimum temperature is 50 degrees Celsius. Stable at 25 and 50 degrees Celsius for 2 h but loses activity at 70 degrees Celsius in 20 min.
Optimum pH is 6.6. Activity is completely abolished above pH 8.6.
Optimum temperature is 70 degrees Celsius. Is very stable at temperatures from 0 to 60 degrees Celsius.
Melting temperature is 65.3 degrees Celsius. Unfolds at 95 degrees Celsius. Partial refolding after cooling back to 25 degrees Celsius.
Optimum temperature is 76 degrees Celsius. Thermostable.
Optimum pH is 7.0. Stable from pH 5.0 to 10.0.
Optimum pH is 9 (PubMed:22709678). Remains active over a wide pH range from pH 7 to 10 (PubMed:22709678).
Optimum pH is 8.2-9.5 using 500 uM folic acid (PteGlu) and 25 uM dl-5-formyltetrahydrofolate (dl-5-CHO-H(4)PteGlu) as substrates.
Optimum temperature is 37 degrees Celsius. Activity completely lost within a few hours at 4 degrees Celsius in the absence of ATP and glycerol.
Optimum pH is 6.0-7.0 for the glucosylceramidase activity (PubMed:11784319, PubMed:17595169). Optimum pH is 6.0 for the hydrolysis of 4-methylumbelliferyl-beta-D-glucopyranoside (PubMed:20728381). Activity decreases sharply with increasing acidity and is less than 4% at pH 4 (PubMed:11784319).
Optimum temperature is 50 degrees Celsius (PubMed:11784319). Stable more than 24 hours at 37 degrees Celsius (PubMed:11784319). Loses activity at 58 degrees Celsius (PubMed:11784319).
Optimum pH is 8-0-9.0.
Optimum pH is from 7 to 8.5.
Optimum pH is 8.0 with Mn(2+) as cofactor and 8.5 with Mg(2+) as cofactor.
Optimum pH is around 9 due to the greater stability of the enamine/imine intermediate at high pH.
Optimum temperature is 42 degrees Celsius. Is moderately thermostable, with a half-life of thermal inactivation of 2 hours at 65 degrees Celsius, and retains 82% activity after incubation for 2 hours at 42 degrees Celsius.
Optimum pH is 6.0 for phosphorolytic activity. Optimum pH is 5.0 for the synthetic reaction.
Optimum pH is 4. Unstable above pH 7.
Optimum pH is 7.5. Activity remains high from pH 6.5 to 9.5. Loses 60% of its activity following incubation at pH 3.5 for 5 minutes.
Only retains 40% of activity after incubation at 60 degrees Celsius for 10 minutes.
Optimum pH is 8.5 (PubMed:17107951, PubMed:33536500). Active between pH 7 and 11 (PubMed:17107951, PubMed:33536500). Optimum pH is 8 for the cleavage of the Cys-Gly dipeptide (PubMed:33303633).
Optimum pH is 5.5 for adenosine and adenine uptake (PubMed:16873718). Absence of adenosine transport at pH 7.4 (PubMed:16873718).
Optimum pH is 8.0-9.5 for the forward reaction and 6.5-7.5 for the reverse reaction.
Optimum pH is near 7.4.
Shows a 3-fold greater lysophosphatidylcholine acyltransferase activity at pH 4.0 than at pH 7.0.
Optimum pH is 7.5-8.0. The optimal activity is at pH 8.8 for zinc-containing form and pH 9.0 for cobalt-containing form.
Optimum pH is 6.5 with sweet gum methylglucuronoxylan as substrate.
Optimum temperature is 45 degrees Celsius with sweet gum methylglucuronoxylan as substrate.
Stable and active at pH 3-8, but loses its activity above pH 9 (PubMed:30763082). Shows reduced activity in phosphate buffer at pH 4-6 (PubMed:30763082).
Optimum pH is 7-9 for sucrose cleavage with ADP as substrate.
Optimum temperature is 60 degrees Celsius for sucrose degradation with UDP and 70 degrees Celsius with ADP. The enzyme remains stable for 10 minutes at 50 degrees Celsius, while addition of ADP, UDP or sucrose enhances thermal stability by 5 degrees Celsius.
Optimum pH is 7.3 for chorismate mutase activity.
Optimum pH is 9.5 for inositol 2-dehydrogenase activity.
Optimum temperature is 95 degrees Celsius. Highly thermostable.
Optimum temperature is 0-25 degrees Celsius.
Optimum pH is 5.5 with sinigrin as substrate.
Optimum pH is 5.5 for the C-terminal fragment DNase.
Optimum temperature is 37 degrees Celsius for the C-terminal fragment DNase.
Optimum pH is 9.0. Active from pH 5.0 to 11.0.
Optimum temperature is 35 degrees Celsius. Active from 20 to 70 degrees Celsius.
Optimum pH is 3.0. Retains 80% and 60% of the original activity after incubation for 30 days at pH 3.0 and pH 4.0 respectively. Unstable at pH higher than 5.0.
Optimum temperature is 45 degrees Celsius. Thermostable up to 50 degrees Celsius. Retains 44% of the original activity after incubation for 30 days at 50 degrees Celsius.
Optimum pH is 6.5 with pNP-alpha-D-maltoside as substrate.
Optimum temperature is 85 degrees Celsius with pNP-alpha-D-maltoside as substrate. Remains stable after preincubation for various periods up to 48 hours at temperatures between 50 and 90 degrees Celsius, pH 6.5. The half-life of the enzyme at 95 degrees Celsius is 2 hours.
Optimum pH is 7.4 (for proline transport).
Optimum temperature is 40 degrees Celsius. Active up to 55 degrees Celsius.
Optimum pH is 7.0-9.0 with Ala-Gln as substrate at 37 degrees Celsius. Activity is very low below pH 5.5 and at pH 10.0 is about 50% of the maximum.
Optimum temperature is 50 degrees Celsius with Ala-Gln as substrate at pH 8.0. Activity is 30% of maximum at 20 degrees Celsius and 50% of maximum at 70 degrees Celsius. Stable when incubated for 10 minutes at temperatures of up to 70 degrees Celsius.
Optimum temperature is 50 degrees Celsius. It remains fully active after 30 minutes of preincubation at 50 degrees Celsius, however, after preincubation at 60 degrees Celsius, the residual activity is only 15%.
Optimum temperature is 90 degrees Celsius (PubMed:9988755). Highly thermostable, has a half-life of 220 minutes at 90 degrees Celsius (PubMed:9988755).
Transport is pH-dependent, with a steep decline at pH values about 5.0.
Optimum pH is 8.5. Activity is null at pH 7.0 and increases as the pH increases from 7.0 to 8.5.
Optimum pH is 7.5-8.0 for 9-cis retinol dehydrogenase activity.
Optimum temperature is 77 degrees Celsius. At 37 degrees Celsius, shows about 20% activity as compared with the maximal value.
Stable at low pH.
With magnesium ions the highest turnover of riboflavin is observed between pH 6 and 7.5, while that of FAD is between 7.0 and 9.0. With zinc ions a steady increase in riboflavin turnover is observed between pH 4.5 and 10, while FAD is the major product at pH 7.0 and decreases rapidly above pH 8.0.
Optimum pH is 8.5, active between pH 7 and 9.5.
Optimum pH is 4.8 (PubMed:33892036). Active from pH 4 to 5 (PubMed:33892036). Activity is significantly reduced at pH 6 and is not observed at pH 7 (PubMed:33892036).
Optimum temperature is 70 degrees Celsius (PubMed:33892036). Thermostable (PubMed:33892036). Highly stable at 50, 60 and 70 degrees Celsius (PubMed:33892036).
Optimum pH is 7.2 (PubMed:3510672). Optimum pH is 9.0. Active at alkaline pH.
Optimum temperature is 60 degrees Celsius. Highly active from 20 to 80 degrees Celsius.
Optimum temperature is 28-34 degrees Celsius.
Optimum pH is 9.5 with spermine as substrate.
Optimum pH is between 10 and 11.
Optimum pH for the reduction of 6-hydroxynicotinate is 6.5. Activity declines rapidly below pH 5.6.
Optimum temperature is 46 degrees Celsius.
Optimum temperature is about 70 degrees Celsius. Active from 4 to 100 degrees Celsius. Thermostable.
Optimum pH is 6.5 in the presence of 1 M NaCl. Active from pH 6 to 9.
Optimum temperature is 50 degrees Celsius. More than 78% of maximal activity is observed at 40 degrees Celsius and 60 degrees Celsius.
Optimum pH is 6.5-7.0 with L-ribulose as substrate.
Optimum temperature is about 80 degrees Celsius with L-ribulose as substrate. Is hyperthermostable, with an apparent melting temperature (Tm) of 102.4 degrees Celsius.
Retains 50% of its antimicrobial activity after heating at 100 degrees Celsius for 100 minutes, and retains 25% antimicrobial activity after heating at 121 Celsius for 15 minutes.
Optimum pH is 7.5 with Leu-pNA as substrate. Strong activity is still detectable at pH 6 and 9.
Optimum temperature is 100 degrees Celsius over a broad pH array. At temperatures lower than 70 degrees Celsius, less than 10% of the maximum activity is detected. Highly thermostable. Shows half-lives of 24.8 minutes and 10.03 hours when incubated at 100 and 80 degrees Celsius, respectively.
Stable between 25 and 95 degrees Celsius.
Optimum pH is 4.7. Active at acidic pHs but inactive in the neutral pH range.
Active up to 70 degrees Celsius at pH 4.1.
Optimum temperature is 34 degrees Celsius.
Optimum temperature is 30 to 35 degrees Celsius with glucose-pentaacetate as substrate. Active from 20 to 50 degrees Celsius. Still exhibits 40 to 70% of the maximum activity after 20 hours of incubation at 50 degrees Celsius.
Optimum pH is about 7.5. Retains > 70% maximum activity at pH 9.0, but displays only 6% activity at pH 4.5.
Optimum temperature is about 45 degrees Celsius.
Optimum pH is 2.5-5.
Optimum pH is 7-8 with salicylic acid as substrate (PubMed:14630969). Optimum pH is 7.5 with benzoic acid as substrate (PubMed:14630969).
Optimum pH is 5 for the XET activity. Optimum pH is 4.5 - 4.75 for the XEH activity.
Optimum pH is between 5.5 and 6.0.
Optimum temperature is between 30 and 35 degrees Celsius.
Optimum pH is 6.4 for homodimer and 6.5 for NAD-MEH heterodimer. In the presence of coenzyme A (CoA), optimum pH is 6.8 for NAD-MEH heterodimer.
Stable to pH variation.
Optimum pH is 8.0 for L-Leu.
Optimum temperature is 4 degrees Celsius.
Optimum temperature is 70 degrees Celsius. Retains more than 90% activity after incubation at 65 degrees Celsius for 1 hour.
Optimum pH is 3.2.
Optimum temperature is 60 degrees Celsius. Has a high thermal denaturation point. The Tmax of the protein was estimated to be 78.2 degrees Celsius.
Optimum pH is 8.3-8.6.
Optimum pH is 5.5-8.0, but decreases linearly above pH 8.3, and shows an inflection at pH 5.5.
High methylation activity of the 50S ribosomal subunit at 37 degrees Celsius. Loss of activity at 42 degrees Celsius.
Stable below 40 degrees Celsius and pH 8.0 for 30 min.
Optimum pH is 6.3-8.3.
Apoprotein in organic solution has an unusual stability towards heating to 100 degrees Celsius.
Optimum pH is 6.5. Active from pH 4.5 to pH 8.5.
Optimum temperature is about 60 degrees Celsius. Is active over a wide temperature range, from 25 to 90 degrees Celsius. Has half-lives of about 30 and 10 minutes at 80 and 90 degrees Celsius, respectively.
Optimum pH is 10.5 for malate oxidation and 8.0 for oxaloacetate reduction.
Optimum temperature is 40 degrees Celsius. Thermostable up to 44 degrees Celsius, in the presence of Leu thermostable to 59 degrees Celsius, in the presence of Leu and 3-methyl-2-oxobutanoate or Leu and acetyl-CoA thermostable until 61-62 degrees Celsius.
Optimum pH is 9.5 for the oxidation reaction and 6.5 for the reduction reaction.
Optimum pH is 8.5-9. Active from pH 8 to 10. Rapidly loses its activity when incubated at pH in the range between 6.5 and 11 for 24 hours.
Optimum temperature is 45-55 degrees Celsius. The activity drops quickly at temperatures below 30 degrees Celsius.
Optimum pH is 8.0. Stable at pH 5.5-7.5. Most active at 7.3 with Pro-beta-naphthylamide (Pro-2-NNap) as the substrate. More than 85% of the activity remains between pH 6.5 and 7.5 after incubation for 15 minutes at 30 degrees Celsius and it retains 50% of the original activity after incubation at pH 7.0 and at 30 degrees Celsius for 30 minutes. 50% of the activity remains after 15 minutes preincubations at pH 8.0.
Maximum activity at 40 degrees Celsius. Stable at temperatures up to 38 degrees Celsius.
Optimum pH is 4.5 for the peroxidase reaction, 6.0 for the catalase reaction, and 8.75 for the NADH oxidase reaction.
Optimum temperature is over 95 degrees Celsius. The enzyme shows a nearly linear increase in activity between 37 and 95 degrees Celsius, with a 6-fold increase between these temperatures. Is highly thermostable, displaying a half-life of 150 minutes in boiling water.
Optimum temperature is 18 degrees Celsius.
Optimum temperature is 37 degrees Celsius. Retains 90% of its activity after 72 hours of incubation at 20 degrees Celsius or -4 degrees Celsius, but loses 50% of its activity after 12 hours of incubation at 37 degrees Celsius.
Optimum pH is 7.2. Active from pH 6.0-8.0, activity decreases rapidly at more acidic pH values. Stable only above pH 5.6.
Optimum pH is between 8 and 8.5 for ATPase activity and between 9 and 9.5 for PPPase activity. Hydrolysis of ATP declines sharply as the pH is increased to 9.5 or decreased to below 7.0.
Optimum pH is 6.5 at 37 degrees Celsius with hexadecanoyl-CoA as substrate.
Optimum pH is 8.4-8.8 for the hydrolysis of 2-pyrone-4,6-dicarboxylate.
Optimum temperature is 65 degrees Celsius. Remains fully active following incubation for 1 hour at 80 degrees Celsius.
Optimum pH is 4-6.5 for p-nitrophenyl-beta-d-cellotrioside (pNP-G3), and 4-8 for p-nitrophenyl-beta-d-cellopentaoside (pNP-G5).
Optimum temperature is approximately 25-45 degrees Celsius. High activity is maintained over a wide temperature range.
Optimum pH is 9.75 with 4-aminobutanal as substrate.
Active at pH 8.4 and inactive at pH 7.5.
Optimum pH is between 5.6-6.2.
Optimum temperature is 60 degrees Celsius (PubMed:16262702). At physiological temperatures (30 degrees Celsius), retains 60-65% of its activity (PubMed:16262702).
Optimum pH is about 2.5.
Optimum temperature is 65 degrees Celsius. Thermal denaturation midpoint (Tm) is 74.5 degrees Celsius.
Optimum pH is 2-4. Stable from pH 2 to pH 6.
Optimum temperature for ATPase activity is 70 degrees Celsius.
Optimum pH is 6 to 8.
Optimum pH is 7.2. Active from pH 6.0 to 9.0.
Stable over a wide pH range, activity is unaffected after incubation at pH 4.0-11.0.
Thermostable. Activity is unaffected by heating at 80 degrees Celsius for 30 minutes, half of the original activity is retained after heating at 100 degrees Celsius for 30 minutes.
Optimum pH is near 7.5. 30% of maximal activity at pH 6.5 and 8.5.
Optimum pH is 6.2-6.6.
Optimum temperature is 90-100 degrees Celsius.
Optimum pH is 7.5 for 2-deoxy-D-ribose 5-phosphate cleavage.
Activity increases from pH 6.5 to 9.
Optimum temperature is 40 degrees Celsius (in vitro, with the purified enzyme).
Optimum pH is 6.0 for the oxidation of 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid and 8.4 for the oxidation of syringaldazine.
Optimum temperature is 45-50 degrees Celsius. Highly thermostable.
Optimum pH is between 7.0 and 9.5.
Optimum pH is 6. Inactive above pH 6.7.
Optimum pH is 6.0 with thermospermine as substrate (PubMed:24105034). Optimum pH is 8.5 with spermine as substrate (PubMed:24105034).
Optimum temperature is 30 degrees Celsius with thermospermine as substrate (PubMed:24105034). Optimum temperature is 35 degrees Celsius with spermine as substrate (PubMed:24105034).
Thermostable, retains about 70% of its activity at 60 degrees Celsius.
Optimum pH is 6.6-7.5.
Optimum temperature is 70 degrees Celsius. Is thermostable up to 90 degrees Celsius.
Thermostable. Fully active at 60 degrees Celsius.
Thermostable at neutral and acidic pHs.
Optimum pH is 11-12.
Optimum temperature is 37 degrees Celsius. Stable up to 45 degrees Celsius.
Optimum temperature is 50-55 degrees Celsius. Highly thermostable. Is half-inactivated at about 67 degrees Celsius.
Optimum pH is 8.5. Active over the pH range 5.5-11.0. Stable from pH 6.5 to 7.5 for 20 hours at 20 degrees Celsius.
Stable below 45 degrees Celsius for 20 minutes at pH 7.0.
Thermostable. Retains about 45% of its activity after treatment of 90 degrees Celsius for 30 minutes.
Optimum pH is 4-10.
Optimum pH is 7.5 for (+)-caryolan-1-ol synthesis.
Optimum temperature is 30 degrees Celsius for (+)-caryolan-1-ol synthesis.
Specific activity decreases significantly at 5 degrees Celsius compared to specific activity at 37 degrees Celsius.
Optimum temperature is 25-40 degrees Celsius (PubMed:19810726). Optimum temperature is 35-55 degrees Celsius (PubMed:15968570).
Optimum pH is 7.2 for the forward reaction and 7.0 for the reverse reaction.
Optimum pH is 10.5 (at 25 degrees Celsius).
Optimum temperature is 37 degrees Celsius. Retains 90% of its activity after 30 minutes at 55 degrees Celsius.
Optimum pH is 3.4.
Thermostable. Retains 48% activity at 50 degrees Celsius, and 13% activity at 100 degrees Celsius.
Thermostable. Fully active after heating 10 min at 65 degrees Celsius and retains half its catalytic activity after 10 min at 72 degrees Celsius. Inactive after heating 10 min at 78 degrees Celsius.
Optimum pH is 7.5 for iron transport.
Optimum pH is 7.6 for oxaloacetate reduction and 9.6 for malate oxidation (PubMed:21439955, PubMed:19277715). Stable between pH 6.8-8.5 for the forward reaction (PubMed:19277715).
Has highest activity between 5-40 degrees Celsius. No activity at 60 degrees Celsius.
Stable below pH 9.0. Degrades by incubation at pH 9.5 and 10.0 for 1 hour.
Relatively stable below 30 degrees Celsius, but aggregates above 40 degrees Celsius. Flocculent precipitate appears by incubation at 70 degrees Celsius for 30 min.
Optimum pH is around 8.
Optimum temperature is around 40 degrees Celsius.
Optimum pH is between 7-9.
Activity is stable between 20-40 degrees Celsius, decreases at higher temperatures and is lost at 70 degrees Celsius.
Optimum pH is 8.7-8.9.
Optimum temperature is 31 degrees Celsius.
Optimum pH is from 5 to 7.
Optimum pH is 7 for 2,5-hexanedione reduction, and 10 for the reverse reaction.
Optimum temperature is 40 degrees Celsius for 3-chloro-1-phenyl-1-propanol reduction, and 54 degrees Celsius for 2,5-hexanedione reduction, and 30 degrees Celsius for the reverse reaction.
Optimum pH is 7.1-9.2.
Optimum temperature is 50-60 degrees Celsius. Is stable at 60 degrees Celsius and loses most of its activity at 70 degrees Celsius.
Optimum pH is 10.5 for the oxidative reaction with L-BD and 6.0 for the reductive reaction with (S)-acetoin. Stable from pH 7.0 to 9.0.
Stable up to 37 degrees Celsius but is rapidly inactivated over 40 degrees Celsius.
Optimum pH is around 7.0.
Optimum temperature is 30 degrees Celsius. Stable up to 40 degrees Celsius.
Optimum temperature is 70 degrees Celsius. 50 perscent maximal activity is exhibited at 75 degrees Celsius. Reaction time was 10 min.
Optimum pH is 4.75.
Thermolabile, displays irreversible loss of activity and aggregation at room temperature.
Optimum pH is 7.5 in phosphate buffer and 8.0 in TRIS-HCl.
Stable at 30 degrees Celsius.
Optimum pH is 7.5 for the forward reaction and 7.0-7.4 for the reverse reaction.
Optimum temperature is 5-37 degrees Celsius.
Optimum pH is 4.5 for maltose hydrolysis, and 5.5 for glucogen hydrolysis.
Optimum temperature is above 40 degrees Celsius.
Optimum pH is approximately 7.5.
Optimum pH is 10-11.
Optimum pH is 6.0 (tag-less variant) (PubMed:31316802). Optimum pH is 5.8 (C-terminally tagged variant) (PubMed:25341489).
Optimum temperature is 50-55 degrees Celsius (tag-less variant) (PubMed:31316802). Optimum temperature is 42 degrees Celsius (C-terminally tagged variant) (PubMed:25341489).
Optimum pH is 7.8 for the condensation reaction and 8.1 for the reverse cleavage reaction.
Remains active between pH 2 and 10.
Remains active after incubation at 121 degrees Celsius for 1 hour.
Optimum temperature is between 38 and 42 degrees Celsius.
Stable from pH 2.0 to 4.0. Activity decreases gradually with increasing pH.
Optimum temperature is 37 degrees Celsius. Stable from 4 to 37 degrees Celsius.
Optimum pH is 5.4-5.9.
Optimum pH is 10. Active from pH 7 to 11.
Optimum temperature is 60 degrees Celsius. Active from 30 to 70 degrees Celsius.
Retains over 90% of its activity between pH 5.6 and 7.0. Retains 85% of its activity at pH 4.0 and 60% of its activity at pH 12.0.
Full activity is retained between 25 and 45 degrees Celsius.
Stable when incubated at 50 degrees Celsius for 10 minutes at pH 5.5. Activity decreases by 50% when incubated at 60 degrees Celsius for 10 minutes at pH 5.5.
Optimum pH is 6.8-8.2.
Optimum temperature is 50 degrees Celsius. Thermolabile above 50 degrees Celsius.
Optimum temperature is 80 degrees Celsius. Thermostable. Half-life at the optimal temperature is 53 minutes.
Thermostable. Active at 46 degrees Celsius.
Thermostable. Most of the activity for GGPP synthase remains even after heating at 60 to 70 degrees Celsius for 100 minutes, although 90% of the enzyme activity is lost on incubation at 90 degrees Celsius for 10 minutes.
Thermostable up to 50 degrees Celsius.
Optimum temperature is 30 degrees Celsius. Activity declines with increasing temperature, with loss of stability at 90 degrees Celsius.
Optimum pH is above 8. No activity is observed under pH 6.
Optimum pH is 9.9.
Thermostable at 50 degrees Celsius for at least 5 minutes.
Optimum pH is 8.5-9.5 for PAP phosphatase activity, 9.3 for IMPase activity, and about 10 for FBPase activity. IMPase activity shows a dramatic decrease at low pH. In contrast, IMPase activity is highly stable at alkaline pH, with more than 60% activity remaining at pH 12.
Optimum temperature is 62 degrees Celsius for IMPase activity. After preincubation at 70 degrees Celsius for 2 minutes and then being assayed at 37 degrees Celsius, more than 95% activities of IMPase and FBPase are lost.
Stable up to about 65 degrees Celsius.
Optimum pH is 7.0. Significant loss of activity at pH 5.0 or under and at pH 11.0 or higher.
Optimum temperature is 30 degrees Celsius. Denaturates at 45 degrees Celsius. Retains about 60% of the maximal activity at 16 degrees Celsius.
Optimum pH is 7.4. Active from pH 5.0 to 10.0.
Optimum temperature is 37 degrees Celsius. Active from 25 to 50 degrees Celsius.
Optimum pH is 8.0 for NAD(P)H oxidase activity.
Optimum pH is 7.0 at 55 degrees Celsius.
Optimum temperature is 55 degrees Celsius at pH 7.0.
Optimum pH is 7.0 for farnesyl diphosphatase activity.
Optimum temperature is 90 degrees Celsius. Is extremely thermostable. No loss of activity is observed after incubation for 2 hours at 80 degrees Celsius. The times required for 50% loss of activity are about 60 minutes at 90 degrees Celsius, 10 minutes at 95 degrees Celsius, and 1 minute at 100 degrees Celsius.
Optimum pH is 10-10.5 (PubMed:12297000). Optimum pH is 9.0 (PubMed:17976651). Reaction rates under neutral conditions are <40% of the maximum (PubMed:12297000).
Optimum pH is 5 for manganese oxidation reaction, and around 3 for all the manganese-independent reactions.
Optimum pH is between 7.2 and 7.5.
Optimum temperature is 60 degrees Celsius and half of the activity is lost on treatment at 65 degrees Celsius for 10 minutes.
Optimum pH is 9.0. Stable from pH 6.0 to 9.5.
Optimum temperature is 45 degrees Celsius, activity decreases rapidly above 45 degrees Celsius possibly due to instability at higher temperatures. Inactivated following 5 minutes incubation at 55 degrees Celsius, and only retains 25% of activity after 5 minutes incubation at 50 degrees Celsius.
Optimum pH is 7.3-8.7 with 4'-nitrophenyl beta-N-acetyl-D-glucosaminide as substrate. Rapidly inactivated above pH 9.5 and stable from pH 6.0 to 9.5.
Optimum temperature is 30-45 degrees Celsius.
Optimum pH is 10.75 with 4-aminobutanal as substrate. Optimum pH is 10.5 with 3-aminopropanal as substrate.
Optimum pH is 8.0 for ATP hydrolysis activity. Loses 50% of the activity at pH 7.0 or 8.5, has less than 20% activity at pH 6.5 and virtually no activity at pH 9.0.
TM is 66 degrees Celsius for phosphorylated protein and 62 degrees Celsius for unphosphorylated protein.
Optimum pH is 4.5-6.0. Maintains 80% activity at pH 4.0-7.5.
Optimum temperature is 52 degrees Celsius. Maintains more than 80% activity at 55 degrees Celsius.
Optimum temperature is 25 to 37 degrees Celsius.
Optimum pH is 8.5. Exhibits a dramatic drop in activity at low pH, with less than 50% activity remaining at pH 7. In contrast, the activity is extremely stable at alkaline pH, with more than 50% activity remaining at pH 12.
Optimum temperature is 80 degrees Celsius. Loses 80% activity after a 2 minutes incubation at 70 degrees Celsius.
Optimum pH is 6.0-6.5. Stable between pH 5.0 and 7.5.
Optimum temperature is 60-70 degrees Celsius. Stable up to 55 degrees Celsius.
Optimum pH is 7.3-7.5.
Optimum pH is 6.3-6.4.
Optimum temperature is about 65 degrees Celsius.
Active up to 60 degrees Celsius.
Optimum pH 3.0.
Optimum pH is 6.5-7.5 with guanosine as substrate. At pH higher than 7.5, there is a marked reduction in reaction rate and a steep drop at pH higher than 9. Below pH 6.5, there is a dramatic decrease in activity reaching virtually zero at pH 6.0. With xanthosine as substrate, the pH optimum is 5.8-7.2. In the reverse reaction with guanine or xanthine as substrates, the pH optimum is 6.5-8.0. The pH dependence of inosine cleavage does not vary between pH 6 and 8. Maximal activity with inosine as substrate is observed at pH 6.6.
The half-life at 45 degrees Celsius between pH 5 and 8 is 5 to 9 minutes.
Optimum pH is 6. Active from pH 5 to 8.
Optimum temperature is 37 degrees Celsius (at pH 6).
Optimum pH is 6.0-7.8.
Optimum pH is 9. Is stable for pH values from 6.0 to 10.0.
Is stable at temperatures below 37 degrees Celsius. The stability of the enzyme decreases sharply at temperatures over 40 degrees Celsius.
Optimum pH is around 5.0 with estrone 3-sulfate, dehydroepiandrosterone sulfate and taurocholate as substrates (PubMed:12724351, PubMed:14610227, PubMed:20507927, PubMed:22201122). The high-affinity site for estrone 3-sulfate binding is pH-dependent with increased transport at lower pH, while the low-affinity site is pH-independent (PubMed:22201122). Taurocholate is only transported at acidic pH (PubMed:14610227).
Optimum temperature is 37 degrees Celsius with estrone 3-sulfate as substrate.
Stable up to a pH of 4.
Optimum pH is 8.25. Stable at pH 7.5 and 4 degrees Celsius in the presence of DTT, EDTA and NaN(3) for at least one month. 57% and 72% of the maximum activity observed at pH 7.5 and 9.0, respectively.
Optimum temperature is 37 degrees Celsius. 75% and 51% of the maximum activity remains at 30 and 45 degrees Celsius, respectively, but the activity decreases rapidly at temperatures above 55 or below 20 degrees Celsius.
Optimum pH is 6-7.5 for the reverse reaction.
Optimum pH is >8.5.
Optimum pH is 5.5-5.
Optimum pH is 7.9-8.5 for CTIL substrate and geranylgeranyl transferase activity.
Optimum temperature is 30-37 degrees Celsius for CTIL substrate and geranylgeranyl transferase activity.
Optimum pH is around 7.
Optimum pH is around 9.7.
Optimum temperature is between 50 and 55 degrees Celsius.
Optimum pH is about 9 for D-sorbitol oxidation, and 7.4 for D-fructose reduction.
Optimum pH is 7.8 for the gamma-replacement reaction.
Optimum pH is 8.0-8.5. Reversibly inactivated below pH 4.5.
Thermostable. No loss of activity occurs after incubation for 2 hours at 60 degrees Celsius. Inactivated after incubation for 2 hours at 75 degrees Celsius, however 45% of activity remains after incubation for 20 minutes at 75 degrees Celsius.
Optimum pH is 8.0 with guaiacol as the reducing agent.
Optimum pH is 5.0 for the hydrolysis of bile acid glucosides.
Optimum pH is 7-7.8.
Optimum pH is 6 (at 50 degrees Celsius).
Optimum temperature is over 95 degrees Celsius.
Optimum temperature is 9 degrees Celsius.
Optimum pH is 4-12. Important decrease in activity at pH 2.
Optimum temperature is 4-90 degrees Celsius. Weak decrease in activity at 100 degrees Celsius.
Has a half-life of 42 hours at 50 degrees Celsius. When TTHB246 is in complex with TTHB247, its half-life is reduced by approximately 30 hours.
Stable as a monodisperse organelle up to 50 degrees Celsius, at higher temperatures they aggregate.
Optimum pH is around 11.0 for the autophosphorylation reaction with the Fe(III)-OH(-) bound form of the enzyme. At pH 8.0, displays 75% of the optimal rate. Is inactive below pH 5.5 and above pH 12.7.
Optimum pH is 8.3-8.9.
Optimum pH is 4.5-5.5. No activity above pH 8.8.
Optimum temperature is 33-49 degrees Celsius. Retains 25% of its maximal activity after heating 10 minutes at 80 degrees Celsius.
Optimum pH is 5.5. Stable from pH 5 to pH 8.
Not thermostable. Approximately 25% of maximum activity remains after 1 hour incubation at 55 degrees Celsius.
Optimum temperature is 80 degrees Celsius at 2.2 and 3.1 M NaCl, and 60 degrees Celsius at 0.9 and 1.3 M NaCl.
Optimum pH is 5.0-6.0 for the forward reaction and 7.0-7.5 for the reverse reaction.
Stable at 35 degrees Celsius. Rapid inactivation occurs at 50 degrees Celsius.
Thermostable. Retains hemagglutinating activity after incubation at 70 degrees Celsius for 1 hour. At higher temperatures activity drops drastically and is lost at 100 degrees Celsius.
Optimum temperature is 105 degrees Celsius. Activity observed at 100 degrees Celsius is about 8 times that at 37 degrees Celsius. Thermostable up to 95 degrees Celsius. Retains full activity after heating at 90 degrees Celsius for 10 minutes and more than 95% of the full activity at 100 degrees Celsius for 10 minutes. Has a half-life of 220 minutes at 100 degrees Celsius. Inactive after heating at 110 degrees Celsius for 30 minutes.
Optimum pH is 3.67-8.
Optimum temperature is 65 degrees Celsius. Half the activity is retained for 25 hours at 40 degrees Celsius and for 5 hours at 50 degrees Celsius.
Optimum pH is 4.5-9.0. High activity is also seen over the range pH 2.0-4.5. Activity decreases with increasing pH above pH 9.0.
Optimum temperature is 4-65 degrees Celsius. Activity decreases rapidly at higher temperatures, nearly 40% of activity is retained at 100 degrees Celsius.
Optimum temperature is 30 degrees Celsius. The enzyme is heat stable.
Optimum pH is 7.5 for cytosolic pH.
Optimum pH is 10.5 with valine as substrate.
Stable in 3 M NaCl for several days at room temperature, but approximately 18% and 50% of specific activity is lost when samples are stored overnight at 4 and -20 degrees Celsius, respectively. Activity is irreversibly lost within minutes in low salt buffers. Activity low, but detectable in 30% sorbitol in the complete absence of salt and it is stable in 30% sorbitol for at least four months at -20 degrees Celsius, but at room temperature approximately 20% of specific activity is lost after 48 hours.
Optimum pH is 6.0. Active between pH 4.0-6.5.
Optimum temperature is 60 degrees Celsius. Could be higher with a more thermally stable substrate.
Optimum pH is 8 with cinnamic acid as a substrate.
Optimum pH is 7.25 to 7.5.
Optimum temperature is 24-26 degrees Celsius.
Active from 60 to 80 degrees Celsius.
Optimum pH is 8.3-8.8 for the forward reaction, and 7 for the reverse reaction.
Optimum temperature is approximately 100 degrees Celsius.
Optimum pH is 6.5 - 7.
Optimum pH is 7.0 for the hydrolysis of nicotinamide. The enzyme activity decreases rapidly below pH 6.0 or above pH 8.0.
Optimum temperature is 40 degrees Celsius for the hydrolysis of nicotinamide. Below 25 or above 70 degrees Celsius, the enzyme loses its activity rapidly.
Optimum pH is 8.0 with L-ornithine as substrate (PubMed:11736657). Optimum pH is 6.8 with L-lysine as substrate (PubMed:11736657).
Optimum pH is 8.0 to 9.0.
Optimum temperature is 37-55 degrees Celsius.
Optimum pH is 6.3 for proteolytic activity with AAF-MCA as substrate.
Optimum temperature is 84-97 degrees Celsius for proteolytic activity (PubMed:8626329, PubMed:16535492). Thermostable. Displays a half-life of 19 minutes at 95 degrees Celsius (PubMed:8626329).
Optimum temperature from 12 to 18 degrees Celsius. Cold tolerant.
Optimum pH is 8 for casein degradation. Active in the broad pH range from 6.5 to 8.5.
In the presence of CaCl(2), the enzyme is fully stable for up to 40 minutes at 70 degrees Celsius, whereas karilysin incubated without calcium loses 50% of its activity within 30 minutes.
Optimum pH is 5-6.5. Active fom pH 5 to pH 9.
Optimum pH is 10. Stable from pH 5 to pH 11.
Optimum pH is 5.5-6.0. Activity decreases slowly above pH 6.0, to approximately 35% of the maximum at pH 8.5. Inactive below pH 4.5.
Optimum temperature is 50 degrees Celsius. Stable below 40 degrees Celsius.
Activity is stable at 90 degrees Celsius for at least 30 minutes.
Optimum temperature is 60 degrees Celsius (PubMed:8421318). Thermostable (PubMed:10383424).
Optimum pH is between 7.5-8.
Optimum temperature is 55-70 degrees Celsius.
Optimum pH is 5.5. The enzyme is slowly inactivated above pH 8.5 and below pH 5.5.
Optimum temperature is 55 degrees Celsius. The enzyme is inactivated at temperatures above 45 degrees Celsius.
Optimum pH is 8.06.
Activity increases linearly up to 40 degrees Celsius. Stable up to 55 degrees Celsius after incubation for 30 min at pH 7.5. During incubation at 25 degrees Celsius for 16h, the enzyme is stable between pH 5.5 and 9.0, where 80% activity is observed.
Optimum temperature is 75 degrees Celsius. Stable at 80 degrees Celsius.
Optimum pH is 5.5. Stable between pH 5.5 and 6.5.
Optimum pH is around 11.6.
Optimum pH is 7.6. Active from pH 6 to 8.9.
Optimum pH is 6.5 for uptake activity. Optimum pH is 9.2 for antiporter activity.
Optimum pH is 6.0 for 2-deoxy-D-ribose 5-phosphate cleavage.
Optimum pH is 5. Highly active at acidic pH range 4-7. Retains over 80% of maximum activity at 4.0. At higher pH values the activity decreases markedly, with more than 50% of activity lost at pH 8.0.
Optimum temperature is 37 degrees Celsius (PubMed:34033194). Optimum temperature is 35 degrees Celsius (Ref.3).
Optimum pH is below 6.5 at low ATP concentrations and is 7.4-7.8 at high ATP concentrations.
Optimum pH is 6.5 with para-nitrophenyl beta-D-glucopyranoside (pNPGlc) as substrate.
Optimum temperature is 45 degrees Celsius with para-nitrophenyl beta-D-glucopyranoside (pNPGlc) as substrate.
Optimum temperature is 47.5 degrees Celsius.
Optimum pH is 6.5-12.
Optimum temperature is 37-75 degrees Celsius.
Active from pH 4.5 to 8.
High levels of activity within a broad pH range of 5.0-9.0.
Optimum pH is 8.5. Active from pH 7 to pH 10.
Optimum pH is 8.5. Active from pH 6 to 8.5.
Optimum temperature is 65 degrees Celsius. Activity decreases sharply above 70 degrees Celsius.
Optimum pH is 7-8.5 with 4,2',4'-trihydroxychalcone as substrate, at 25 degrees Celsius.
Optimum pH is about 7.3.
Thermostable up to at least 50 degrees Celsius.
Optimum temperature is 50 degrees Celsius. Activity increases from 30 to 50 degrees Celsius, the enzyme is inactive at 70 degrees Celsius.
Optimum temperature is 94-96 degrees Celsius. Over 96 degrees Celsius, the activity decreases dramatically. Hyperthermostable. The half-life of activity is 30 minutes at 95 degrees Celsius and increases to 2 hours at 90 degrees Celsius.
Optimum pH is between 6 and 8 for glyoxalase activity (PubMed:21696459, PubMed:7848303). Significant inhibition of glyoxalase activity below pH 5 (PubMed:21696459). Optimum pH is 8 for aminopeptidase activity (PubMed:15550391).
Optimum temperature for glyoxalase activity is around 37 degrees Celsius (PubMed:21696459). Optimum temperature for aminopeptidase activity is around 43 degrees Celsius (PubMed:15550391).
Optimum pH is 4.4.
Optimum pH is 8.0. Active from pH 5.0 to at least 9.0.
Optimum temperature is 30-45 degrees Celsius. Is stable between 0 and 35 degrees Celsius after incubation of 30 minutes.
Optimum pH is 9.6.
Optimum temperature is 37 degrees Celsius. The activity is abolished at 70 degrees Celsius.
Optimum temperature is slightly lower than 40 degrees Celsius.
Optimum pHs are 5.5 and 8.0.
Very sensitive to pH. A pH change from 8 to 6 results in greater than 30- to 200-fold decreases in its activity. However, deactivation at pH 6 is reversed by shifting the enzyme to pH 7 or 8.
Optimum pH is 7.0. for ATPase activity.
Optimum pH is 4.5-6.5.
Optimum pH is between 8-10 for the hemagglutinating activity of the human erythrocytes.
Hemagglutinating activity of the human erythrocytes is fully maintained after heating at 50 degrees Celsius for 3 hours. The activity is partially lost after heating at 55 degrees Celsius and completely lost after heating at 65 degrees Celsius for 30 minutes.
Shows extremely high thermostability. Retains full activity after incubation at 80 degrees Celsius for at least 2 hours.
Optimum pH is 8-9 for degradation (PubMed:1512253).
Optimum temperature is 40 degrees Celsius activity drops off quickly from 45 degrees Celsius (PubMed:1512253).
Optimum pH is 10.0-10.5 for the oxidative deamination of L-alanine and about 9.0 for reductive amination of pyruvate.
Loses about 50% of its initial activity when heated at 65 degrees Celsius for 5 min.
Optimum temperature is 85 degrees Celsius. Active from 60 to 100 degrees Celsius.
Optimum pH is 9.0 for the forward reaction and 7.5 for the reverse reaction.
Optimum pH is 3.5. Maintains more than 50% of the maximum activity at pH 2.5-6.0. Not active at a pH above 7.0.
Optimum temperature is 30 degrees Celsius. Retains approximately 20-40% of its maximum activity even at 0-5 degrees Celsius. Drastic loss of activity at temperatures above 45 degrees Celsius, at which the half-life of the enzyme activity is less than 10 min.
50% activity is retained after 1 hour at 100 degrees Celsius or 3 hours at 95 degrees Celsius.
Optimum pH is 7.5. Enzyme activity is not detected at pH values above 9.0.
Optimum pH is 7. It maintains more than 70% of its optimal activity even at pH 5.0. PcpD loses its activity quickly in basic solutions, retaining less than 20% of its optimal activity at pH 8.0.
Denaturation temperature (Td) is 98.3 degrees Celsius.
Optimum temperature is 100 degrees Celsius for phosphatase and kinase activities. Both are inactive below 60 degrees Celsius.
Optimum pH is 6.5 for both lactose and ONPG hydrolysis. Stable at pH 5-8 and most stable at 6.5, retaining more than 90% and 80% of its activity when incubated at pH 6.5 and 37 degrees Celsius for 5 days and 1 month, respectively.
Optimum temperature of the activity is 50 degrees Celsius when using both lactose and ONPG as substrates under 10 minutes assay conditions. Stable over a wide range of temperatures (4-42 degrees Celsius), and when kept at these temperatures up to 1 month. Most stable at 37 degrees Celsius, retaining 90% of its activity after 1 month at this temperature. Half-life time of activity of approximately 7 days, 5 hours, and 30 minutes at 55, 60 and 65 degrees Celsius, respectively. Approximately 45% of lactose is hydrolyzed within the first 3 hours of the reaction at 60 degrees Celsius, while about 20% is cleaved at 37 degrees Celsius when employing initial lactose concentration of 50 g/l at pH 6.5. For initial lactose concentrations of 200 and 50 g/l and at temperature of 60 degrees Celsius, the maximum GOS yields are approximately 12% and 7%, respectively. At the initial lactose concentration of 200 g/l, the GOS yields obtained at 60 degrees Celsius are significantly higher than at 37 degrees Celsius, with approximately 12% and 5% of total sugars, respectively.
Optimum pH is 3.7-4.7 for birchwood xylan.
Optimum pH is 9.0 (isoform 1), 8.0 (isoform 3). Isoform 1 is less sensitive to pH. Isoform 1, isoform 2 and isoform 3 all retain some activity at pH 9.5.
Isoform 1 and isoform 3 are active from 45 to 60 degrees Celsius.
Optimum temperature is 37 degrees Celsius. Loss of catalytic activity at temperatures above 50 degrees Celsius.
Optimum pH is 7.0-8.5 with stevioside as substrate (at 40 degrees Celsius).
Optimum temperature is 40 degrees Celsius with stevioside as substrate (at pH 7.0).
Optimum pH is 7.0. Active at pH 4.0 to pH 9.0. Stable from pH 5.5 to pH 7.0.
Optimum temperature for activity is 30 degrees Celsius. Stable below 40 degrees Celsius. Above 45 degrees Celsius the activity is sharply reduced.
Transport activity is maximal at acidic pH (<6.0) and almost negligible at pH >7.5.
Optimum pH is 8.0 with p-nitrophenyl alpha-galactoside as substrate.
Optimum pH is 6. Maintains 70% or more of the maximal activity from pH 6.0 to 9.0.
Optimum temperature is 50 degrees Celsius. Maintains 70% or more of the maximal activity from 35 to 65 degrees Celsius.
Optimum pH is 8.2. Stable when incubated for 10 minutes between pH 2 and 11. Retains 70% of its activity when incubated for 10 minutes at pH 1.5.
Stable after incubation at 70 degrees Celsius for 10 minutes. Retains 80% of its activity after incubation at 80 degrees Celsius for 20 minutes.
Optimum pH is 2.6. Retains 95% and 79% of the original activity after incubation for 30 days at pH 3.0 and pH 10.0 respectively.
Optimum temperature is 55 degrees Celsius. Thermostable up to 50 degrees Celsius. Retains 60% of the original activity after incubation for 30 days at 50 degrees Celsius.
Optimum pH is 7.0. Stable between pH 6.0 and 9.8.
Optimum temperature is 40 degrees Celsius. Stable at temperatures lower than 50 degrees Celsius.
Optimum temperature is 37-38 degrees Celsius.
Optimum pH is 5.7-7.
Optimum pH is 9.0. Activity is stable from pH 6 to 11 but decreases sharply below pH 6.
Optimum temperature is 60 degrees Celsius. Activity is stable from 40 to 80 degrees Celsius.
Optimum pH is 6 (PubMed:34467865). Retains over 72 percent activity in the pH range 5 to 8 (PubMed:34467865). Retains activity above 89% after incubation at pH 6 for 72 hours (PubMed:34467865). Retains activity above 61% after incubation at pH 5-8 for 72 hours (PubMed:34467865).
Optimum temperature is 55 degrees Celsius (PubMed:34467865). Thermostable (PubMed:34467865). Retains over 84 percent activity when incubated for 3 hours at temperatures between 10-60 degrees Celsius (PubMed:34467865).
Optimum pH is 6-7 without NaCl at 37 degrees Celsius.
Optimum temperature is 48 degrees Celsius in 50 mM MOPS-NaOH buffer (pH 7.0) without NaCl.
Optimum pH is 5.0: Stable between pH 3.0 and 7.0.
Optimum pH is 5.5. Stable from pH 3 to pH 8.
Shows a maximum of activity at pH 8.5 in the oxidation of ursocholate, and between pH 7.0 and 8.0 when tested in the reduction reaction of 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate, with a gradual drop on the alkaline side and a sharp drop on the acidic side.
Optimum pH is 10.1.
Optimum temperature is 70.0 degrees Celsius.
Optimum pH is 4.0. Inactive below pH 3.0 and above pH 6.5. The half-life (t1/2) values for activity loss at 30 degrees Celsius are 150 min at pH 6.6, 5.5 min at pH 6.8 and 14 min at pH 2.4.
Remains fully active after heating at 50 degrees Celsius and pH 4.0 for 10 min. Retains 65% of its activity after heating at 55 degrees Celsius for 10 min. The half-life value for loss of activity at 60 degrees Celsius and pH 4.0 is 3.5 min.
No loss of activity below 10 degrees Celsius.
Optimum pH is 4.5 for the peroxidase reaction.
Optimum pH is 5.68 for both catalytic activities. Stable between pH 5.0-6.3.
Stable up to 40 degrees Celsius for both catalytic activities.
Optimum pH is 4.5-5.2.
Optimum is pH 6.0 using PNPG or TOS as substrate. Not active below pH 5.
Optimum temperature is 50 degrees Celsius using PNPG as substrate (PubMed:15480628). Optimum temperature is 35 degrees Celsius using TOS as substrate (PubMed:10742215). Stable at 40 degrees Celsius. Half-life time at 50 degrees Celsius is 10 minutes.
ATPase activity is stable from 25 to 35 degrees Celsius.
Optimum pH is 1.5.
Stable up to about 47 degrees Celsius.
The half-life at 58 degrees Celsius is 50 minutes.
Optimum pH is 10. Stable from pH 5.0 to 11.0.
Optimum temperature is 30 degrees Celsius. Stable up to 37 degrees Celsius and loses activity almost completely when incubated at 60 degrees Celsius for 10 minutes.
Optimum pH is 5.0 (with polyphosphate as substrate).
Optimum pH is 6.5 at 37 degrees Celsius for bacteriolytic activity. Optimum pH is 3 at 40 degrees Celsius for chitinase activity.
Optimum pH is 9.0. No activity at or below pH 7.5.
Optimum pH is 7-8. Has approximately 80% maximal activity at pH 6.5 and 40% activity at pH 8.5.
Optimum temperature is 10 to 18 degrees Celsius.
Optimum pH is 7.8, with half-maximal activities at pH 6.5 and 8.5.
Optimum pH is 6.5 for 2-deoxy-D-ribose 5-phosphate cleavage.
Optimum pH is 5.5-8.4. Maintains 80% activity at pH 5.0-9.0.
Optimum temperature is 44 degrees Celsius. Maintains more than 80% activity at 50 degrees Celsius.
Optimum pH is 7. It is active in a pH range of 5.5-9.0.
Optimum temperature is 45 degrees Celsius. No activity is detected above 50 degrees Celsius.
Optimum pH is 8.0 - 9.5.
Optimum temperature is 25-35 degrees Celsius for N-terminal 209 residue fragment.
Optimum pH is 4.0 when inulin is a substrate and 5.0 when sucrose is a substrate.
Optimum temperature is 60 degrees Celsius when inulin is a substrate and 55 degrees Celsius when sucrose is a substrate.
Optimum pH is between 8 and 8.5.
Is very stable at 20-30 degrees Celsius.
Stable from pH 2.0 to 12.0.
Retains almost 100% of its activity after heating for 10 minutes at 70 degrees Celsius, activity is lost rapidly above 70 degrees Celsius.
Optimum pH is 7-7.3 in both synthetic and cleavage directions. Is active over a broad pH range, from pH 5.0 to 9.0, in both directions. Maintains 5 to 10% activity in the synthetic direction above pH 11.0. The enzyme is also stable at basic pHs, where it maintains around 80% residual synthetic activity after 15 days of incubation.
Optimum temperature is 60 degrees Celsius for synthetic activity and up to 70 degrees Celsius for the cleavage reaction. Is very thermostable, maintaining 80% of cleavage activity after 48 hours at 60 degrees Celsius. However, at higher temperatures (70 degrees Celsius), activity decreases to less than 10% in 8 hours. Temperature stability is further improved by the presence of stabilizing additives.
Optimum pH is 8.0 for CHA aldolase activity, and 7.0 for oxaloacetate decarboxylase activity.
Optimum pH is approximately 7. Stable activity from pH 4.5 to 8.5. Retains more than 30% of activity at pH 9.0.
Highest activity at 45 degrees Celsius. Has about 40% of activity at 55 degrees Celsius.
Exhibits activity across a broad pH range. Enzyme activity is moderately improved under acidic conditions.
Optimum temperature is 37-45 degrees Celsius. Rapidly inactivated after exposure for 10 minutes at 55 degrees Celsius.
Optimum pH is between 7.9 and 8.4.
Optimum pH is 6.6. Significant activity is observed over the pH range of 6.4 to 7.8. Enzymatic activity decreases markedly at pH 8.0 and at pH 6.2 and below.
Stable up to about 53 degrees Celsius.
Optimum pH is 7.5 with monobutyl phthalate as substrate. Retains 50% of maximum activity between pH 6.5 and pH 7.8.
Optimum temperature is 40 degrees Celsius with monobutyl phthalate as substrate.
Optimum pH is 4.5 for phytase activity (PubMed:8387749, PubMed:10696472). Optimum pH is 2.5 for acid phosphatase activity (PubMed:6282821). Optimum pH is 2.5-3.0 for acid phosphatase activity (PubMed:10696472).
Optimum temperature is 55 degrees Celsius for phytase activity (PubMed:8387749). Optimum temperature is 60 degrees Celsius for phytase activity (PubMed:10696472).
Activity decreases as pH is lowered from neutral to acidic.
Optimum pH is 9.5. Active from pH 8.5 to 10.0.
Optimum pH is 5.0-6.0 with insulin B chain as substrate and at 37 degrees Celsius.
Optimum pH is 9.6 (isoform 2).
Optimum pH is 8.5 for the transport of MPP (PubMed:11388889). While optimum pH is 6.0 for the transport of the drug quinidine, no transport activity is observed at pH 7.5, possibly due to the protonation of quininide at pH6.0 (PubMed:11408531).
Optimum temperature is 22 degrees Celsius for the transport of TEA. Doesn't show any transport activity of TEA at 4 degrees Celsius.
Optimum temperature is 85-100 degrees Celsius.
Optimum temperature is 37 degrees Celsius. Active across a broad temperature range.
Gradually increased from pH 6.5 to 8.5 in its IDP hydrolyzing activity.
Exhibited a temperature-dependent increase in its IDP hydrolyzing activity up to 60 degrees Celsius.
Heat-stable protein that continues to cause erythematous skin eruption in the patient and to recognize IgE after heating.
Optimum pH is 5.0 for leucine racemase activity.
Optimum temperature is 70 degrees Celsius for D-leucine to L-leucine reaction. Optimum temperature is 75 degrees Celsius for L-leucine to D-leucine reaction.
Optimum temperature is 50 degrees Celsius (at pH 6.5).
Optimum pH is 7.0. Active from pH 6.0 to 9.0.
Optimum pH is 4.5-5 (PubMed:8112342). Optimum pH is 4.4 with either cholesterol oleate or trioleoylglycerol as substrate (PubMed:7204383).
Denaturation starts at 45 degrees Celsius. At temperatures above 75 degrees Celsius, begins to precipitate, and eventually becomes irreversibly denatured. Cannot be resolubilized in aqueous solution after being heated up to 95 degrees Celsius and then cooled down to 25 degrees Celsius.
Optimum pH is 9.3. Above pH 10, stability decreases rapidly.
Optimum temperature is 10 degrees Celsius. Above 65 degrees Celsius no activity is detected.
Optimum pH is 7.4. Active within the pH range of 4.5-8.5.
Optimum temperature is 80 degrees Celsius. Active at a broad temperature range (60-90 degrees Celsius). Highly thermostable with a half-life of 25 hours at 70 degrees Celsius, 9 hours at 80 degrees Celsius, and 6 hours at 90 degrees Celsius. Shows a Tm of 112.7 degrees Celsius.
Optimum pH is 7.1-10.
Optimum pH is 5.5. Has low activity at pH 7 and is inactive at pH 8.
Optimum pH is 6 for hemagglutinating activity.
Heat stable. Retains hemagglutinating activity after incubation at 100 degrees Celsius for 30 minutes.
Optimum pH is 5 with carboxymethyl cellulose as substrate. Retains 80% activity at pH 6, and about 50% at pH 7. Inactive at pH values below 4 and above 9.
Optimum temperature is 70 degrees Celsius. Retains about 85% activity after 24 hours at 65 degrees Celsius. Retains at least 50% activity after 4 hours at 70 degrees Celsius. Rapidly looses activity at 75 degrees Celsius.
Stable at 40 degrees Celsius and below, but it loses activity rapidly at temperatures above 45 degrees Celsius.
Optimum temperature is 30 degrees Celsius. Active from 30 to 55 degrees Celsius.
Optimum pH is 6. Stable between pH 5-9.
Optimum temperature is 75 degrees Celsius. Retains about 55% activity after 15 minutes at 65 degrees Celsius.
Optimum pH varies depending on the substrate used and phosphate concentration. In the absence of inorganic phosphate pH optima are 6.6 for L-4-carboxy-4-hydroxy-2-oxoadipate, 8.0 for D-4-carboxy-4-hydroxy-2-oxoadipate, 6.7 and 8.0 for DL-4-carboxy-4-hydroxy-2-oxoadipate, 8.3 for DL-4-hydroxy-4-methyl-2-oxoglutarate, 9.3 for DL-4-hydroxy-2-oxoglutarate and 8.8 for oxaloacetate. In the presence of 3 mM inorganic phosphate pH optima are more alkaline: 8.2 for L-4-carboxy-4-hydroxy-2-oxoadipate, 8.6 for D-4-carboxy-4-hydroxy-2-oxoadipate, 8.2 for DL-4-carboxy-4-hydroxy-2-oxoadipate, 8.9 for DL-4-hydroxy-4-methyl-2-oxoglutarate, 9.4 for DL-4-hydroxy-2-oxoglutarate and 8.9 for oxaloacetate. Stable at pH 6.0 to pH 9.5.
Retains 50% of maximum activity after incubation at 54 degrees Celsius for 10 minutes.
Optimum pH is 3.0-4.2.
Optimum pH is 8.4-9.2.
Loses half of its hemagglutinating activity after heating at 50 degrees Celsius for 120 minutes. Activity is abolished by heating at 80 degrees Celsius for 60 minutes.
Optimum pH is 8.5 with L-lysine as substrate.
Optimum pH is 6.0. Active from pH 4.5 to 10.5.
Optimum pH is 8.1-8.4.
Optimum pH is 6-8 for hemagglutinating activity. Activity is reduced to 50% at pH 8.5.
Optimum pH is 7.0-7.5. Stable from pH 5.0-11.0. Retains 65% and 70% of activity respectively when incubated at pH 4.0 and 12.0 for 24 hours.
Optimum temperature is 40 degrees Celsius. Stable below 40 degrees Celsius. Loses 70% and 99% of activity respectively when incubated for 10 minutes at 50 and 60 degrees Celsius.
Active at pH 7.0 and below.
Thermostable up to 50 degrees Celsius. Thermostabilized in the presence of both fructose 1,6-bisphosphate (FBP) and Mn(2+) ions.
Antibacterial activity against P.aeruginosa is retained between 25 and 90 degrees Celsius.
Optimum pH is 7.0 for both kinase and phosphorylase reactions. Activity decreases to less than 10% of the maximal activity at pH 5.5.
Optimum pH is 6.5 with 2,6-DMP as substrate (PubMed:11867755). The pH dependence of the reaction as monitored by oxygen consumption shows a broad activity peak between pH 5 and 6 and a second activity peak at pH 8 (PubMed:12794077).
Optimum temperature is 55 degrees Celsius with 2,6-DMP as substrate.
Optimum pH is 7.2 with Zn(2+) and protoporphyrin IX as substrates.
Optimum pH is 6.7-7.2.
Optimum temperature is 40 degrees Celsius. Thermostable for 10 min up to 45 degrees Celsius.
Optimum pH is 6.0-9.0. Below pH 6.0 the enzyme is reversibly inactivated, above pH 10.0 the enzyme is irreversibly inactivated.
Optimum pH is 2.5 for Arg-Agm exchange.
Optimum temperature is between 60 and 70 degrees Celsius.
Optimum temperature is 50 degrees Celsius. Retains about 80% of the activity after incubation for 2 hours at 50 degrees Celsius, but is rapidly inactivated at higher temperatures.
Optimum pH is 7-9. Active from pH 5.5 to pH 9.5.
Optimum pH is 7.0. Significant activity is observed from pH 5.5 to pH 7.5.
Optimum pH is 5.2 (PubMed:16624575). Activity decreases sharply at pH above 6 (PubMed:16624575). Optimum pH is 5.5-7.5 (PubMed:18312598).
Optimum pH is about 9.
Optimum pH is 8.0 for both the forward and reverse reactions.
Activity is pH-independent.
Optimum pH is 8.0-9.0. Stable between pH 7.0 and 8.5.
Stable below 55 degrees Celsius.
Optimum pH is 6.6 for both activities.
Sensitive to extremes of pH.
Optimum pH is 6.6 for homodimer and 6.5 for NAD-MEH heterodimer. In the presence of coenzyme A (CoA), optimum pH is 6.8 for both homodimer and NAD-MEH heterodimer.
DNA polymerase optimum pH is 6.5, very thermostable, has a half-life of 12 hours at 95 and 3 hours at 100 degrees Celsius, for protein cloned without inteins in E.coli.
DNA polymerase optimum temperature is 75 degrees Celsius, for protein cloned without inteins in E.coli (PubMed:9361436). Both intein endonucleases are thermostable (PubMed:9742242).
Optimum pH is 6 for the XET activity.
Optimum temperature is between 90 and 95 degrees Celsius. The activity is undetectable at temperatures below 60 degrees Celsius.
Optimum pH is 4.5-5.5 (PubMed:1369024). Stable from pH 3.0 to 8.5 at room temperature and from pH 4.0 to 7.5 at 40 degrees Celsius (Ref.5).
Optimum pH for FAEE synthesis is 7.0. Optimum pH for PNPB-hydrolyzing activity is 6-8.
Optimum pH is 6 for short chain and medium-chain triacylglycerol and 4 for long-chain triacylglycerol (PubMed:1935982). Inactivated when pH is below 1.5 (PubMed:1568562).
Optimum pH is 7.5. Exhibits more than 50% of its optimal activity between pH 6.5 and 9.0. Displays highest stability in a pH range of 7.0-8.0.
Optimum temperature is 35 degrees Celsius. At this temperature, its half-life is about 20 hours. Retains about 50% and 20% of its initial activity after 20 h and 48 h of incubation at 35 degrees Celsius, respectively.
Optimum pH is 7.5. Activity is extremely low below pH 6.5. Activity decreases slightly at more basic pHs, activity at pH 9.0 was only 10% less than that found at pH 7.5.
Optimum pH is between 4.0 and 4.5.
Optimum temperature is 50 degrees Celsius (PubMed:33820885). Maintains above 50% of the maximum activity between 40 degrees Celsius and 70 degrees Celsius (PubMed:33820885).
Shows a constant increase in activity until 60 degrees Celsius using butyraldehyde as substrate.
Optimum pH is 7.6-10.6 with L-fucose or D-arabinose as substrate.
Optimum temperature is 50 degrees Celsius with L-xylulose as substrate.
Optimum temperature is higher than 95 degrees Celsius. Still active after 120 minutes at 100 degrees Celsius.
Optimum pH is 8 with acetoacetyl-CoA and 3-oxooctanoyl-CoA as substrates.
Optimum pH is 8.0 for sodium and lithium transport. Exhibits Na(+)/H(+) and Li(+)/H(+) antiporter activity between pH 6.5 and 9.5.
Highly active between pH 6.5 and 9.0. Retains 50% and 10% of maximum activity at pH 5.0 and 4.5 respectively.
Optimum temperature is 37 degrees Celsius. The activity at 30 and 45 degrees Celsius is 58% and 87% of the optimal activity, respectively. Exceptionally high temperature stability.
Optimum pH is 8.5 with L-homoserine as substrate.
Optimum pH is about 6.5. Active from pH 5 to 9. Stable from pH 3 to 11.
Optimum temperature is about 50 degrees Celsius. Active from 30 to 65 degrees Celsius. Thermostable for 30 minutes up to 55 degrees Celsius.
Optimum pH is 7.0 (PubMed:12620340). Optimum pH is 7.5 (PubMed:27135507).
Optimum temperature is 35-37 degrees Celsius (PubMed:12620340, PubMed:27135507). Optimum temperature is 37 degrees Celsius (PubMed:27135507).
Optimum pH is 7-8 (lipase activity in the presence of bile salts) and 7.0-7.25 (lipase activity in the absence of bile salts).
Optimum temperature is 90 degrees Celsius. The enzyme is highly thermophilic. 50% activity is still observable at 103 degrees Celsius.
Optimum pH is 5.5-6.0. Active from 3.5 to 8.5 with phytic acid as substrate. The optimum pH is shifted to more acidic values with 4-nitrophenyl phosphate as substrate.
Optimum pH is 7.5-8.0. 60% of the activity is retained at pH 11.0 (PubMed:19332543). No autolysis at pH 4.0-9.0 in the presence of 5 mM EDTA or 5 mM CaCl(2) (PubMed:19805099).
Thermostable (PubMed:19332543, PubMed:27451395, PubMed:19805099). Activity is unaffected by heating to 60 degrees Celsius and only partially reduced after incubation at 70 degrees Celsius (PubMed:19332543).
Active from pH 4 to 12.
Optimum pH is 7.5. Half maximum activity is seen at pH 6.9 and 8.1.
Active from pH 6.5 to 7.8.
Optimum pH is 6.5. Active from pH 5 to 8.
Optimum pH is 4.5-5.5. Inactive above pH 7.5.
Optimum pH is 7.5. Stable in the range of pH 6.0-8.0.
After heating at 40 degrees Celsius, the remaining activity is 40% of the original activity.
Optimum pH is 9.5-10.5. Is inactive at pH values of 7 or less.
Loses less than 10% of the initial activity during a 10 minutes incubation at 65 degrees Celsius. At 80 degrees Celsius, 90% of the activity is lost over the same time period.
Optimum pH is 8.0 for the hydrolysis of p-nitrophenyl hexanoate.
Optimum temperature is 39 degrees Celsius for the hydrolysis of p-nitrophenyl hexanoate.
Highly thermostable. Loss of stability only after incubation for 1 hour at 100 degrees Celsius.
Fully active at 80 degrees Celsius. Gradually loses some activity from 90 to 100 degrees Celsius.
Optimum temperature is around 90 degrees Celsius (PubMed:20370616). Thermostable (PubMed:20370616, PubMed:25862541). The extreme thermal stability of this enzyme is due to the presence of high intersubunit associative forces supported by extensive H-bonding and ionic interactions network (PubMed:25862541).
Optimum pH is 3.5-4.0 (PubMed:1369024). Stable from pH 2.5 to 8.5 at room temperature and from pH 2.5 to 4.5 at 40 degrees Celsius (Ref.3).
Optimum pH is 6.0 (PubMed:1765081). Optimum pH is 4.0 (PubMed:7852356).
Optimum temperature is 55-65 degrees Celsius (PubMed:1765081, PubMed:7852356). Thermostable up to 40 degrees Celsius (PubMed:7852356).
Optimum pH is 7.5-8.5. Activity decreases sharply below pH 7.0 and is null at pH 6.5.
Fairly stable between pH 6 and 9.
Optimum pH is 5.0. Retains about 76 percent of its activity after being incubated at pH 2.0 for 30 min at 37 degrees Celsius.
Optimum temperature is 50 degrees Celsius. Displays about 95 percent of peak activity in the temperature range from 37 to 41 degrees Celsius.
Optimum pH is 5.5 for adenosine transport (PubMed:15701636, PubMed:19164483, PubMed:28729424). No adenosine transport at pH 7.4 (PubMed:28729424).
High levels of activity within a broad pH range of 5.0-8.0.
Heat-stabile at 70 degrees Celsius. Displays a half-life of 20 minutes at 82 degrees Celsius.
Optimum temperature is 29 degrees Celsius (PubMed:12084074). Stable at 10 degrees Celsius, but above this temperature the activity decreases. The enzyme is completely inactivated at 40 degrees Celsius (PubMed:6052627, PubMed:12084074).
Stable from pH 6 to 9.5.
Thermostable up to 40 degrees Celsius.
Optimum pH is 9.5. Active from pH 6 to pH 10.5. Stable from pH 5 to pH 10.
Thermostable for 3 hours up to 80 degrees Celsius.
Optimum pH is 9.0-11.0.
Optimum temperature is 80 degrees Celsius. Retains about 40% activity after 15 minutes at 65 degrees Celsius.
Optimum pH is about 9.5.
Optimum pH is 4 for phosphatase activity using p-nitrophenol phosphate as a substrate.
Optimum pH is 7.5. Activity is greater than 50% of maximal from pH 6.2 to 9.2. Is stable throughout a broad range of pH (6-10).
No decrease in activity observed after incubating at pH 2.5 and pH 7.4 for 1 hour. Retains 20% activity after incubation at pH 12 for 1 hour.
No decrease in activity observed after heating for 1 hour at up to 80 degrees Celsius. Retains 20% activity after incubation at 95 degrees Celsius for 1 hour.
Optimum pH for hemolytic activity is 10. Hemolytic activity increases with increasing pH from pH 7 to pH 10, but almost no hemolytic activity is observed below pH 6.5 (PubMed:7876091, PubMed:8663224). Binds to lactose between pH 4-10 (PubMed:9058193, PubMed:9805377). Pore formation in artificial liposomes containing human erythrocyte membrane lipids lactosyl ceramide (LacCer) or globotetraosylceramide (Gb4Cer) increases with increasing pH (PubMed:10478454). Cytotoxic effect on Madin-Darby canine kidney (MDCK) cell line increases with increasing pH with maximal cytotoxicity at pH 10 at 4 degrees Celsius, but no significant pH effect is observed at 37 degrees Celsius (PubMed:9133626, PubMed:10101284).
Optimum temperature for hemolytic activity is 10 degrees Celsius. Hemolytic activity decreases markedly with increasing temperature (PubMed:7876091). Binds to lactose between 5-40 degrees Celsius, with maximal binding around 10 degrees Celsius (PubMed:9058193). Pore formation in artificial liposomes containing human erythrocyte membrane lipids lactosyl ceramide (LacCer) or globotetraosylceramide (Gb4Cer) increases with increasing temperature (PubMed:9990124). Cytotoxic effect on African green monkey kidney (Vero) and Madin-Darby canine kidney (MDCK) cell lines increases with decreasing temperature (PubMed:9133626).
Optimum temperature is 30-45 degrees Celsius. Is heat-resistant, since the enzymatic activity is not significantly impaired by incubation at 95 degrees Celsius for 1 or 5 minutes.
Optimum pH is 4 with 2,6-dimethoxyphenol as substrate. Retains 90% of its activity after 4 hours at pH 2.5.
Optimum temperature is between 65 and 70 degrees Celsius.
Stable from pH 6 to 10.
Stable at temperatures below 80 degrees Celsius.
Optimum temperature is 98 degrees Celsius.
Optimum pH is 6.5 in the synthesis direction, and more than 50% of the maximum activity is retained within the pH range of 5.5 to 8. In the phosphorolytic direction, the optimum is reached at pH 6.
Optimum temperature is 60 degrees Celsius against azocasein. Retains 50% of the activity by incubation at 60 degrees Celsius for 1 hour.
Optimum pH is 7.5 to 8.0.
Optimum pH is 8.25 with DL-3-hydroxykynurenine as substrate.
Optimum pH is 7.0 for CoA hydrolysis (PubMed:10922370). Optimum pH is 8.0-8.5 for 8-oxo-dGTP hydrolysis (PubMed:15475388).
Optimum pH is 4.3.
Active from pH 6 to 10.
Optimum pH is 7-9 for the forward and reverse reactions.
Retains 75% of the activity after incubation at 75 degrees Celsius for 30 minutes.
Optimum temperature is 22 degrees Celsius. Active between 17 and 32 degrees Celsius.
Optimum pH isin the absence of effectors. Optimum pH is 7 in the presence of tryptophan. Optimum pH is 5 in the presence of tyrosine.
Optimum temperature is <38 degrees Celsius.
Optimum pH is 7-9, varying somewhat depending on the substrate.