['Many bibliographical references suggest reserves of Vibrio cholerae in the aquatic world, strains of both the serovariety no 01 and 01 having been isolated in different parts of the globe in fresh and extensive salt waters.', 'Samples were taken monthly from January to May and from October to December, and every fortnight from June to September. The method of isolation applied is in accordance with the standard set down by the Institute of Health Carlos III, with some specific modifications.', 'During the period 90/91, routine analyses carried out by the Andalusian Service of Health of waters from the area of the Continental Basin in the province of Seville showed the presence of Vibrio cholerae 01. In the same way, during the complete annual cycle Vibrio cholerae no 01 was also isolated.'] Do [ Environmental isolation of Vibrio cholerae 01 in continental waters of the Province of Seville ]?