['Little is known about the association between self-weighing frequency and weight gain prevention, particularly in worksite populations.', 'The degree to which self-weighing frequency predicted 2-year body weight change in working adults was examined.', 'The association between self-weighing frequency (monthly or less, weekly, daily, or more) and 24-month weight change was analyzed in a prospective cohort analysis (n\u2009=\u20091,222) as part of the larger HealthWorks trial.', 'There was a significant interaction between follow-up self-weighing frequency and baseline body mass index. The difference in weight change ranged from -4.4\u2009±\u20090.8\xa0kg weight loss among obese daily self-weighers to 2.1\u2009±\u20090.4\xa0kg weight gain for participants at a healthy weight who reported monthly self-weighing.'] Is self-weighing frequency associated with weight gain prevention over 2 years among working adults?