['Trichophyton rubrum is an important cause of onychomycosis. Molecular strain typing methods have recently been developed to address questions of epidemiology and source of relapse following treatment.', 'To determine whether T. rubrum nail infections are caused by one or more strains of this fungus.', 'Nail specimens from 10 patients with onychomycosis due to T. rubrum were cultured and five colonies per culture plate were selected for molecular strain typing. DNA was extracted from these isolates and subjected to a polymerase chain reaction-based typing method that analyses variations in numbers of repetitive elements in the non-transcribed spacer region of the ribosomal RNA gene repeats.', 'In six of 10 specimens, there were two or more T. rubrum strain types present.'] Does molecular strain typing of Trichophyton rubrum indicate multiple strain involvement in onychomycosis?