['To obtain recombinant 38,000 protein in large quantities and to study its immunologic characteristics by stable expression of the gene encoding for 38,000 antigen of Mycobacterium tuberculosis in E. coli.', 'Expression plasmid was constructed with DNA recombinant technique. Positive clones were screened using double digestion and polymerase chain reaction. Recombinant plasmid was transformed into E. coli. Then E. coli carrying recombinant plasmid were induced. The expression of 38,000 antigen was identified by SDS-polyacrylamid gel electrophoresis (PAGE) and immunoblotting. Stained gel was scanned to detect expression level of recombinant antigen.', 'Gel stained with coomassie blue G-250 showed that the induced E. coli carrying recombinant plasmid can produce 38,000 protein at high level. Gel scan showed that 38,000 antigen expression in E. coli was about 36% - 40% of total cellular protein. The recombinant 38,000 antigen existed mostly in inclusion bodies. The recombinant antigen can react with antibodies: serum of pulmonary tuberculosis patients and the goats immuned with Mycobacterium tuberculosis.'] Is [ The 38,000 protein of Mycobacterium tuberculosis overexpressed in Escherichia coli ]?