['To construct and identify recombinant adenovirus Ad-IRF8 carrying mouse interferon regulatory factor-8 (IRF8) and observe the influence of the over-expressed IRF8 on the differentiation of osteoclast precursor cells (RAW264.7) induced by soluble receptor activator of nuclear factor κB ligand (sRANKL).', 'Mouse IRF8 gene amplified by PCR was subcloned into the shuttle vector pAdTrack-CMV. The vector pAdTrack-IRF8-CMV was linearized by Pme I , followed by homologous recombination with bone vector pAdEasy-1 in E.coli BJ5183. Recombinant adenovirus vector pAd-IRF8 was obtained and linearized by Pac I , and then transfected into AD293 cells. The recombinant adenovirus Ad-IRF8 was harvested after packaged in AD293 cells. RAW264.7 cells were infected by Ad-IRF8. The overexpression of IRF8 was verified with semi-quantitative reverse transcription-PCR (RT-PCR) and Western blotting. The influence of the overexpression of IRF8 on sRANKL-induced differentiation of RAW264.7 cells was detected by tartrate-resistant acid phosphatase (TRAP) staining.', 'The shuttle vector pAdTrack-IRF8-CMV and recombinant vector pAd-IRF8 were successfully constructed. The recombinant adenovirus Ad-IRF8 was also successfully packaged in AD293 cells. The overexpression of IRF8 in RAW264.7 cells was confirmed by RT-PCR and Western blotting. TRAP staining showed that the overexpression of IRF8 effectively inhibited the differentiation of RAW264.7 cells into osteoclast-like cells.'] Does [ Overexpression of mouse IRF8 inhibit the differentiation of RAW264.7 cells into osteoclast-like cells ]?