['Our objective was to determine the effects of ischemia reperfusion (IR) treatment with continuous-mode diagnostic frequency ultrasonography exposure by assessing leukocyte-endothelial cell interactions, in terms of frequency and relative proportions of rolling and firmly attached leukocytes.', 'Studies were carried out in the awake hamster chamber window preparation. Tourniquet ischemia was implemented by compressing a circular ring on the chamber window tissue for 4 hours, followed by reperfusion. Animals were randomly assigned into one of four groups as follows: IR; IR + ultrasonography; IR + N(omega)-nitro-L-arginine methyl ester (L-NAME); and IR + L-NAME + ultrasonography. Venules were exposed to epi-illumination, videorecorded, and leukocytes were categorized as "rolling"; flowing with endothelial contact; or "immobilized" cells, and counted during digital video-playback in 100-microm length segments.', 'Leukocyte interaction with venular endothelium substantially decreased, during longterm reperfusion (p < 0.05) with ultrasonography exposure. Nitric oxide production inhibition, after L-NAME treatment, and ultrasonography exposure resulted in additional earlier substantially decreased leukocyte-endothelial cell interactions (p < 0.05).'] Does diagnostic frequency continuous ultrasonography directly mitigate venular ischemia reperfusion damage?