['The aim of this experiment was to study acute toxicity and local stimulation of cucurbitacinBE poly-lactic acid nano-particles (CuBE-PLA-NP) for targeting cervical lymph node.', 'Various doses of CuBE-PLA-NP lyophilization injection and cucurbitacinBE (CuBE) were hypodermically or veno-injected into the mice, and then the drug toxicity and side effects were observed; 50% lethal dose (LD50) was counted. CuBE-PLA-NP lyophilization injection and CuBE were also injected into the quadriceps femoralis muscle of rabbits to observe the local stimulate responses of drugs.', 'The fifty percent lethal doses of subcutaneous and venous CuBE-PLA-NP in mice were 16.74 mg/kg +/- 1.83 mg/kg and 5.60 mg/kg +/- 0.83 mg/kg respectively, that were higher than those of CuBE which were 8.63 mg/kg +/- 1.00 mg/kg and 2.50 mg/kg +/- 0.30 mg/kg respectively. Local stimulate response in rabbit quadriceps femoralis muscle after CuBE-PLA-NP and CuBE injected are 0 grade and 0.1 grade respectively.'] Do [ Acute toxicity and local stimulate test of cucurbitacinBE polylactic acid nano-particles of targeting cervical lymph nodes ]?