['Adipose tissue plays an important role in the pathogenesis of insulin resistance, obesity, and Type-2 diabetes. Human adipocytes express abundant cholesteryl ester transfer protein (CETP). However, the function and role of CETP in regulating lipoprotein metabolism are mostly unknown. In this study, we examined whether CETP affected the insulin-mediated responses in adipocytes.', 'Because mouse 3T3-L1 preadipocytes do not express CETP, we established a stable cell line expressing human CETP by transfecting the cells with pcDNA3.1/human CETP. We used a standard approach to differentiate the cells into mature adipocytes, and we examined the cholesterol balance and insulin responses.', 'The human CETP stable cell line expressed stable levels of CETP without affecting the expression of either peroxisome proliferator-activated receptor-gamma (PPARγ) or glucose transporter-4 (GLUT4) throughout cell differentiation. CETP expression significantly increased the level of both total and free cholesterol in the mature adipocytes. Upon insulin stimulation, CETP expressing cells had significantly higher protein kinase B (Akt) phosphorylation and 2-(3)H-deoxyglucose uptake, as compared with 3T3-L1 cells and cells transfected with control vector.'] Does human cholesteryl ester transfer protein enhance insulin-mediated glucose uptake in adipocytes?