['To determine if the insulin gene variable number of tandem repeats (VNTR) regulatory polymorphism is associated with hyperandrogenism in a population of Spanish women.', 'Controlled clinical study.', 'Tertiary institutional hospital.', 'Ninety-six hyperandrogenic patients and 38 healthy control women.', 'Whole blood and serum samples were collected during the follicular phase of the menstrual cycle.', 'Insulin gene VNTR regulatory polymorphism genotypes (classes I/I, I/III, and III/III alleles) and serum androgen levels. Insulin resistance was estimated from fasting glucose and insulin levels by using the homeostatic model assessment.', 'The frequencies of VNTR genotypes were 45.5%, 43.3%, and 11.2% for I/I, I/III, and III/III alleles considering patients and controls as a whole. These frequencies were not statistically different in controls (47.4%, 34.2%, and 18.4%) and in patients (44.8%, 46.9%, and 8.3%).'] Is insulin gene variable number of tandem repeats regulatory polymorphism associated with hyperandrogenism in Spanish women?