['There have been many assumptions made about neuronal loss in mammals due to aging. However, when we examined the retinal ganglion cell layer of a marsupial, the quokka, from 0.5 to 13.5 years of age, we found that the total neuron number did not decrease significantly even into extreme old age. The retinal area increased slowly throughout life, leading to a decrease in cell density. Neuronal death in the rat retina has been assumed, since the cell density has been seen to fall with age. However, a similar study to ours in the quokka has never been performed in the laboratory rat, the model for so many experimental investigations.', 'We decided to test the hypothesis that rats do not lose neurons due to aging and that an increase in retinal area, and not cell death, as previously suggested, might underlie the decreasing cell density seen in aging animals such as rats.', 'We kept laboratory rats under standard, unvarying conditions throughout the trial, sacrificing 3 animals every 3 months up to 30 months, and examined the retinal ganglion cell layer through adult life.', 'We found that the numbers of neurons did not decrease in this species, even in the oldest rats. We also saw that the retinal area increased slowly with a concomitant slow decrease in mean neuronal density. Soma diameters of neurons gradually increased throughout life.'] Do numbers of neurons in the retinal ganglion cell layer of the rat change throughout life?