['Clinical observations suggest that repeated injury within a week after a traumatic event impairs the regeneration of tissues. Our aim was to investigate the effect of repeated trauma on the proliferation of satellite cells in skeletal muscle tissue.', "Cold lesion injury was performed in the soleus muscle and in the motor cortex of anesthetized male Wistar rats 0, 1, or 2 times with 7\xa0day intervals between the interventions. Following the last operation, 5-bromo-2'-deoxyuridine was injected i.p. for 6 or 12\xa0days to label dividing cells. Gut epithelium was used as positive control. Immunohistochemistry was performed 1 and 5\xa0weeks after the last injury and the sections were analyzed with confocal microscopy.", 'In the case of repeated trauma, the percentage of proliferating cells remained the same compared to single hit animals after 1\xa0week (28.0\xa0±\xa02.5% and 29.6\xa0±\xa03.0%) as well as after 5\xa0weeks (13.9\xa0±\xa01.8% and 14.5\xa0±\xa02.2%).'] Is muscle regeneration undisturbed by repeated polytraumatic injury?