['To construct a chimeric SEA-hPLAP-1 cDNA with gene splicing by overlap extension.', 'The SEA gene and a DNA fragment encoding the signal for GPI-anchor attachment of hPLAP  -1 were amplified by PCR. The two amplified gene sequence was annealed to form a chimeric GPI-  anchored SEA molecule with gene splicing by overlap extension. The resulting chimera was cloned in pGEM-T vector and verified by sequencing analysis.', 'A chimeric SEA-hPLAP-1 cDNA was successfully constructed with gene splicing by overlap extension.'] Do [ Construction of a chimeric SEA-hPLAP-1 cDNA with gene splicing by overlap extension ]?