['National Football League (NFL) players are exposed to multiple head collisions during their careers. Increasing awareness of the adverse long-term effects of repetitive head trauma has raised substantial concern among players, medical professionals, and the general public.', 'To determine whether low perfusion in specific brain regions on neuroimaging can accurately separate professional football players from healthy controls.', 'A cohort of retired and current NFL players (n\u200a=\u200a161) were recruited in a longitudinal study starting in 2009 with ongoing interval follow up. A healthy control group (n\u200a=\u200a124) was separately recruited for comparison. Assessments included medical examinations, neuropsychological tests, and perfusion neuroimaging with single photon emission computed tomography (SPECT). Perfusion estimates of each scan were quantified using a standard atlas. We hypothesized that hypoperfusion particularly in the orbital frontal, anterior cingulate, anterior temporal, hippocampal, amygdala, insular, caudate, superior/mid occipital, and cerebellar sub-regions alone would reliably separate controls from NFL players. Cerebral perfusion differences were calculated using a one-way ANOVA and diagnostic separation was determined with discriminant and automatic linear regression predictive models.', 'NFL players showed lower cerebral perfusion on average (p\u200a<\u200a0.01) in 36 brain regions. The discriminant analysis subsequently distinguished NFL players from controls with 90% sensitivity, 86% specificity, and 94% accuracy (95% CI 95-99). Automatic linear modeling achieved similar results. Inclusion of age and clinical co-morbidities did not improve diagnostic classification.'] Do perfusion Neuroimaging Abnormalities Alone Distinguish National Football League Players from a Healthy Population?