['To determine the effect of fish oil supplementation on circulating concentrations of adiponectin, leptin, insulin, glucose, triglyceride and cholesterol in healthy dogs.', 'Twenty healthy adult dogs were administered 220 mg/kg of a fish oil supplement once daily for 30 days. At baseline, on supplement and 10 to 20 weeks off supplement, dogs were examined, body condition scores determined (range: 4 to 6), body measurements recorded for % body fat calculation and fasted blood samples collected.', 'Serum concentrations of the measured individual and total n-3 polyunsaturated fatty acids increased following supplementation (P<0·001). Mean serum adiponectin concentration on supplement was 3·4 µg/mL (95% confidence interval: 0·8 to 6·0; P=0·006) higher than baseline, and 5·3 µg/mL (2·0 to 8·7; P<0·001) higher than off supplement. Concentrations of adiponectin off supplement were not different from baseline. There were no significant differences in weight, body condition scores, % body fat and concentrations of other measured analytes between baseline and on supplement.'] Does fish oil supplementation increase concentration of adiponectin in healthy dogs?