['Both RhoA (Rho1) and polo-like kinase 1 (Plk1) are implicated in the regulation of cytokinesis, a cellular process that marks the division of cytoplasm of a parent cell into daughter cells after nuclear division. Cytokinesis failure is often accompanied by the generation of cells with an unstable tetraploid content, which predisposes it to chromosomal instability and oncogenic transformation. Several studies using lower eukaryotic systems demonstrate that RhoA and Plk1 are essential for mitotic progression and cytokinesis.', 'Physical and functional interactions between RhoA and Plk-1 were analyzed using subcellular localization of RhoA and Plk1 in HeLa cells by immunofluorescence and co-precipitation techniques, followed by Western blotting in RhoA transfected cells.', 'Plk1 localizes to kinetochores as well as to spindle poles during prophase and metaphase; it translocates to the midbody during telophase. RhoA is also enriched at the midbody region during telophase and colocalizes with Plk1. Recombinant RhoA, expressed as a GFP fusion protein, is enriched in the nucleus of HeLa and U2OS cells. Ectopically expressed GFP-RhoA does not cause significant cell death, although there exist a group of cells that appear to exhibit a delay in mitotic exit or in impaired cytokinesis.'] Does polo-like kinase 1 regulate RhoA during cytokinesis exit in human cells?