['Many studies have demonstrated that chemokines and their receptors play important roles in breast cancer. However, few of them focus on the concentration change of chemokines along breast cancer evolvement, especially for primary breast cancer.', 'To investigate the effects of chemokines and their receptors on different stage of primary breast cancer, and to find correlationships between chemokines, between different clinico-pathological characters of patients or between chemokines and different clinico-pathological characters of patients.', 'We evaluated and compared the concentration of 10 chemokines and receptors in serum of patients diagnosed as breast benign change, epithelial proliferation (present only or with atypia), in situ carcinoma and invasive carcinoma.', 'Our oneway ANOVA analysis results showed that in all cases from benign diseases to invasive carcinoma, the concentration of CXCL8, CXCR4 and CXCL12 was significantly different; in benign subgroups (benign change, benign change with proliferation, atypia), the concentration of CCL2 and CCR5 was significantly different; in invasive carcinoma cases, DARC concentration was significantly correlated with the relapse risk of patients.'] Do chemokines fluctuate in the progression of primary breast cancer?