['The purpose of this study was to determine the effect of a mixture of amino acids on pituitary responsiveness to a stimulation test (GnRH + CRH) in athletes.', 'In a double blinded counterbalanced experimental protocol, 10 moderately trained male athletes performed the pituitary stimulation test 60 min after a single oral administration of a placebo (P1-AS) or an amino acid mixture solution (AS) (L-arginine hydrochloride 100 mg x kg(-1) + L-ornithine hydrochloride 80 mg x kg(-1) + L-branched chain amino acids 140 mg x kg(-1): 50% L-leucine, 25% L-isoleucine, 25% L-valine) on two different occasions. Plasma ACTH, LH, FSH, GH, and cortisol were evaluated before (-60, -30, 0 min) and after (+15, +30, +45, +60, +90 min) the stimulation test.', 'The ACTH, LH and FSH response to CRH + GnRH was significantly higher in AS group both as absolute values and area under curve (AUC) values than in P1-AS group. Pre-test and post-test cortisol AUC levels were significantly higher in P1-AS group although a higher percent increase in post-test cortisol was found in AS group. The total GH-AUC was higher in AS group and, as expected, the absolute GH concentrations at different time points were not influenced by CRH + GnRH administration.'] Does acute amino acids supplementation enhance pituitary responsiveness in athletes?