['Dentine hypersensitivity is a common problem attributed by patent dentinal tubules. Ingredients incorporated in toothpastes aim to occlude patent dentinal tubules to minimise the dentine hypersensitivity. However, frequent consumption of acidic soft drinks may reverse the dentinal tubules occlusion. In this in vitro study, the efficacy of dentinal tubules occluded by commercially available toothpastes to withstand different duration of an acidic soft drink challenge was investigated.', 'One hundred and twenty dentine discs were divided into three groups. The discs from each group were brushed with toothpaste containing bioactive glass, arginine and control toothpaste. Each group were then divided into four subgroups and exposed to acidic soft drink over four different time durations.', 'The scoring and the percentage of occluded dentinal tubules by Novamin containing toothpaste was significantly better compared to arginine or the control toothpaste. Acidic soft drink challenge reduced the extent of dentinal tubules occlusion along with time. Dentinal tubules occluded by Novamin containing toothpaste withstand the acidic challenge comparatively for longer period.'] Do dentinal tubules occluded by bioactive-glass containing toothpaste exhibit high resistance toward acidic soft drink challenge?