The latest research found that a gene called SIRT1 in the brain guides the synthesis of corresponding proteins in the human thalamus. The SIRT1 gene weakens with age and it gradually reduces the amount of protein that is synthesized. Therefore, the researchers speculated that the gene Will leads to middle-aged happiness. For the above conclusion to be true, which of the following premises must be based on? ['The thalamus is related to controlling weight. The more the corresponding protein in the thalamus is synthesized, the more effectively it can curb weight gain.', 'The older the person, the higher the probability of SIRT1 gene mutation, the mutation will gradually lose the function of guiding protein synthesis.', 'Existing animal experiments show that artificially enhancing the function of the SIRT1 gene can reduce the appetite of the subject and increase the calorie expenditure.', "SIRT1 gene exists in many tissues and organs, and only the gene that exists in the thalamus can affect the body's protein synthesis."]