['The analysis of glucosinolates (GS) is traditionally performed by reverse-phase liquid chromatography coupled to ultraviolet detection after a time-consuming desulphation step, which is required for increased retention. Simpler and more efficient alternative methods that can shorten both sample preparation and analysis are much needed.', 'To evaluate the feasibility of using ultrahigh-pressure liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC-QTOFMS) for the rapid profiling of intact GS.', 'A simple and short extraction of GS from Arabidopsis thaliana leaves was developed. Four sub-2\u2009µm reverse-phase columns were tested for the rapid separation of these polar compounds using formic acid as the chromatographic additive. High-resolution QTOFMS was used to detect and identify GS.', 'A novel charged surface hybrid (CSH) column was found to provide excellent retention and separation of GS within a total running time of 11\u2009min. Twenty-one GS could be identified based on their accurate mass as well as isotopic and fragmentation patterns. The method was applied to determine the changes in GS content that occur after herbivory in Arabidopsis. In addition, we evaluated its applicability to the profiling of other Brassicaceae species.'] Does rapid profiling of intact glucosinolates in Arabidopsis leave by UHPLC-QTOFMS using a charged surface hybrid column?