['To assess the accuracy of surface models derived from 3D cone beam computed tomography (CBCT) with two different segmentation protocols.', 'Seven fresh-frozen cadaver heads were used. There was no conflict of interests in this study. CBCT scans were made of the heads and 3D surface models were created of the mandible using two different segmentation protocols. The one series of 3D models was segmented by a commercial software company, while the other series was done by an experienced 3D clinician. The heads were then macerated following a standard process. A high resolution laser surface scanner was used to make a 3D model of the macerated mandibles, which acted as the reference 3D model or "gold standard". The 3D models generated from the two rendering protocols were compared with the "gold standard" using a point-based rigid registration algorithm to superimpose the three 3D models. The linear difference at 25 anatomic and cephalometric landmarks between the laser surface scan and the 3D models generate from the two rendering protocols was measured repeatedly in two sessions with one week interval.', "The agreement between the repeated measurement was excellent (ICC=0.923-1.000). The mean deviation from the gold standard by the 3D models generated from the CS group was 0.330mm±0.427, while the mean deviation from the Clinician's rendering was 0.763mm±0.392. The surface models segmented by both CS and DS protocols tend to be larger than those of the reference models. In the DS group, the biggest mean differences with the LSS models were found at the points ConLatR (CI: 0.83-1.23), ConMedR (CI: -3.16 to 2.25), CoLatL (CI: -0.68 to 2.23), Spine (CI: 1.19-2.28), ConAntL (CI: 0.84-1.69), ConSupR (CI: -1.12 to 1.47) and RetMolR (CI: 0.84-1.80)."] Does segmentation process significantly influence the accuracy of 3D surface models derived from cone beam computed tomography?