['Immunoregulatory effects of choriocarcinoma-derived factors on leukocytes have been documented. The present study was designed to investigate the effect of JEG-3 culture supernatants on interferon-gamma (IFN-gamma), interleukin-17 (IL-17) and IL-1beta production in the mixed lymphocyte reactions (MLRs).', 'A human choriocarcinoma cell line JEG-3 was used to test the effects of its culture supernatants on the proliferation and cytokine production in the MLRs. The cell proliferation was assessed using the BrdU incorporation and the amounts of cytokines were measured using enzyme-linked immunosorbent assays.', 'The JEG-3 culture supernatants caused significantly reduced IFN-gamma and IL-17 production in the MLRs. However, the supernatants did not influence MLR production of IL-1beta.'] Do jEG-3 cell culture supernatants cause reduced interferon-gamma and interleukin-17 production in mixed-lymphocyte reactions?