['The objective was to analyse the impact of growth conditions, in particular of the specific growth rate, on the resistance of Escherichia coli towards UV-C irradiation.', 'Escherichia coli K12 wild-type bacteria (and in some experiments also a mutant not expressing RpoS, the global regulator of the general stress response; rpoS(-) mutant) were cultivated either in batch culture until stationary phase was reached or in continuous culture at different specific growth rates (μ) and then irradiated with UV-C light. Inactivation was determined by plating. The specific growth rate had a profound effect on UV-C resistance. Stationary phase or very slowly growing cells (0≤μ<0·1 h(-1)) as well as fast-growing cells exhibited a high resistance compared to bacteria growing at an intermediate rate (between 0·2 and 0·4 h(-1) ). The rpoS(-) mutant was more susceptible to UV irradiation than the wild-type when obtained from stationary phase, while mutant cells from continuous culture (μ=0·2 h(-1)) revealed a UV-C resistance similar to the wild-type grown under the same conditions.'] Is uV-C inactivation in Escherichia coli affected by growth conditions preceding irradiation , in particular by the specific growth rate?