['To study methylation aberrations in spermatozoa at developmentally important imprinted regions to ascertain their role in early embryo loss in idiopathic recurrent spontaneous miscarriages (RSM).', 'Case-control study.', 'Academic research setting at National Institute for Research in Reproductive Health, Parel, Mumbai.', 'Male partners of couples with a history of RSM and male partners of couples with proven fertility (control group).', 'None.', 'DNA methylation levels at imprinting control regions of DLK1-GTL2, MEST (PEG1), and ZAC (PLAGL1) by Epityper Massarray and global methylation levels as measured by LINE-1 methylation and anti-5-methyl cytosine antibody in spermatozoa of 23 men in control group and 23 men in RSM group.', 'We did not observe any aberration in the total methylation levels in any of the imprinted genes or global methylation analyzed.'] Is methylation status of imprinted genes DLK1-GTL2 , MEST ( PEG1 ) , ZAC ( PLAGL1 ) , and LINE-1 elements in spermatozoa of normozoospermic men , unlike H19 imprinting control regions , associated with idiopathic recurrent spontaneous miscarriages?