['We tested the hypothesis that maternal low-protein (LP) diet during gestation and lactation can program myostatin (MSTN) signaling and protein synthesis in skeletal muscle of offspring at weaning stage (35\xa0days).', 'Fourteen Meishan sows were fed either LP or standard-protein diets throughout gestation and lactation, male offspring piglets were killed at weaning stage and longissimus dorsi (LD) muscles were taken. The cross-sectional areas (CSA) of LD muscles were measured by hematoxylin and eosin staining. The levels of free amino acids in plasma were measured by amino acid auto-analyzer. Proteins and mRNA were determined by Western blot and RT-qPCR, respectively.', 'Body weight, LD muscle weight and the myofiber CSA were significantly decreased (P\xa0<\xa00.05) in LP piglets; meanwhile, the concentration of branched-chain amino acids was also significantly decreased (P\xa0<\xa00.001). MSTN protein content tended to be higher (P\xa0=\xa00.098) in LP piglets, while the expression of MSTN receptors, activin type II receptor-beta and transforming growth factor type-beta type I receptor kinase, was significantly up-regulated (P\xa0<\xa00.05). Furthermore, p38 mitogen-activated protein kinase, the downstream signaling factor of MSTN, was also enhanced significantly (P\xa0<\xa00.05). In addition, key factors of translation initiation, phosphorylated eukaryotic initiation factor 4E and the 70\xa0kDa ribosomal protein S6 kinase, were significantly decreased (P\xa0<\xa00.05) in LP piglets.'] Does maternal low-protein diet affect myostatin signaling and protein synthesis in skeletal muscle of offspring piglets at weaning stage?