['The influence of high glucose solution on the chemical communication between cardiac cells was investigated in cell pairs isolated from the left ventricle of adult Wistar Kyoto rats. For this, Lucifer Yellow CH was dialyzed into one cell of the pair using the whole cell clamp technique, and the diffusion of dye in the dialyzed as well as in non-dialyzed cell, was followed by measuring the intensity of fluorescence in both cells as a function of time. The results indicated that: 1) high glucose solution (25 mM) disrupted chemical communication between cardiac cells; 2) the effect of high glucose solution was reduced by Bis-1 (10(-9)M) which is a PKC inhibitor, and by enalapril (10(-9)M); 3) intracellular dialysis of Ang II (100 nM) also caused dye uncoupling; 4) calculation of gap junction permeability (Pj) (cm/s) indicated a value of 3 ± 0.07 × 10(-5)cm/s; n=32; (6 animals) for the controls and 0.4 ± 0.86 × 10(-6)cm/s; n=35 (6 animals) (P<0.05) for cells incubated with high glucose solution for 24h; 5) measurements of Pj for cell pairs treated with hypertonic solution plus Bis-1 (10(-9)M) or enalapril maleate (10(-9)M) showed no significant change of Pj (P>0.05).'] Is chemical communication between cardiac cells disrupted by high glucose : implications for the diabetic heart?