['One day after male sexual behavior [one ejaculation or copulation to satiety (ad libitum copulation during 4h with the same female)] androgen receptor immunoreactivity (AR-ir) is decreased and estrogen receptor alpha immunoreactivity (ERα-ir) increased in various brain areas related with its control. Seven days after sexual satiety there was a limited recovery of sexual behavior accompanied by a partial recuperation in the AR-ir. In this study we evaluated if these changes in AR-ir and ERα-ir were paralleled by variations in their respective mRNA.', 'Sexually experienced male rats were sacrificed at different intervals: immediately, 24h or seven days after sexual satiety or 24h after one ejaculation. The changes in AR and ERα mRNA were analyzed by in situ hybridization using digoxigenine-labeled oligonucleotide probes in the MPOA, LSV and the bed nucleus of the stria terminalis, medial division, anterior (BSTMA).', 'AR mRNA density was decreased in the MPOA and the LSV immediately and 24h after one ejaculation or sexual satiety. Seven days after copulating to satiety, there was a recovery of AR mRNA. In the BSTMA the different behavioral conditions did not modify the AR mRNA expression. In the MPOA, LSV and BSTMA the ERα mRNA increased after a single ejaculation and at all intervals after sexual satiety.'] Does copulation modify AR and ERα mRNA expression in the male rat brain?