['The aim of the present study was to investigate the validity of the Lactate Minimum Test (LMT) for the determination of peak VO(2) on a cycle ergometer and to determine the submaximal oxygen uptake (VO(2)) and pulmonary ventilation (VE) responses in an incremental exercise test when it is preceded by high intensity exercise (i.e., during a LMT).', 'Ten trained male athletes (triathletes and cyclists) performed 2 exercise tests in random order on an electromagnetic cycle ergometer: 1). Control Test (CT): an incremental test with an initial work rate of 100 W, and with 25 W increments at 3-min intervals, until voluntary exhaustion; 2). LMT: an incremental test identical to the CT, except that it was preceded by 2 supramaximal bouts of 30-sec (approximately 120% VO(2)peak) with a 30-sec rest to induce lactic acidosis. This test started 8 min after the induction of acidosis.', 'There was no significant difference in peak VO(2) (65.6+/-7.4 ml x kg(-1) x min(-1); 63.8 +/- 7.5 ml x kg(-1) x min(-1) to CT and LMT, respectively). However, the maximal power output (POmax) reached was significantly higher in CT (300.6+/-15.7 W) than in the LMT (283.2+/-16.0 W). VO(2) and VE were significantly increased at initial power outputs in LMT.'] Does the lactate minimum test protocol provide valid measures of cycle ergometer VO ( 2 ) peak?