['Correlations have been found between oxidative stress and hypertension.', 'To determine whether antioxidants can normalize sympathetic dysfunction at pre- or postsynaptic levels in spontaneously hypertensive rats (SHRs).', 'Untreated SHRs and Wistar-Kyoto (WKY) rats were compared with rats treated with melatonin (30 mg/kg per day) or N-acetylcysteine (NAC) (4 g/kg per day) given in drinking water for 4 weeks. At the presynaptic level, SHRs had greater plasma noradrenaline concentrations (P <0.01) and an enhanced release of [3H]noradrenaline from isolated atria (P <0.001). At the postsynaptic level, they exhibited an increased proportion of beta2-adrenoceptors in the heart (P <0.001)and a decrease in the chronotropic and mean arterial pressure (MAP) responses to isoproterenol (P <0.001). Melatonin and NAC decreased MAP (P <0.001) and heart rate (P <0.05), and restored the plasma noradrenaline concentrations (P<0.01 and P <0.001, respectively), the chronotropic response to isoproterenol (P <0.05) and the proportions of beta(1)/beta(2)-adrenoceptors in the heart (P <0.05) in SHRs to the levels found in WKY rats. The same treatments decreased the release of [3H]noradrenaline from isolated atria (P <0.05), and melatonin slightly improved the relaxation in the aorta in SHRs only (P <0.05). Plasma concentrations of adrenaline, the isoproterenol-induced relaxation in mesenteric arteries, the total density and affinity of beta-adrenoceptors in the heart, and the adenylate cyclase reactivity of cardiac membranes to isoproterenol, forskolin, sodium fluoride and guanylylimidophosphate were not altered by the treatments.'] Does chronic antioxidant treatment improve sympathetic functions and beta-adrenergic pathway in the spontaneously hypertensive rats?