['Proteomic biomarkers may be useful for monitoring therapeutic response and to triage cancer patients to best therapy.', 'In this review, we highlight the importance of specimen acquisition, preparation and analysis in radiotherapy proteomic studies. We also discuss practical approaches for the design and execution of clinical proteomic studies using our recent experience based on specimens accrued during prostate cancer radiation therapy.'] Does practical approach to proteomic biomarkers within prostate cancer radiotherapy trials?