['To determine in vitro the loss of integrity caused on photocatalytic anatase coated implant surfaces by clinical instrumentation through changes in surface topography and loss of functionality.', 'Anatase-coated titanium discs were treated with diamond burs, polishers, plastic and metal hand instruments, air scaler and air flow devices. The pressure exerted through instrumentation was measured online. Surface topography was evaluated through scanning electron microscopy and contact profilometry, surface function through hydrophilization capacity upon UV-A activation.', 'Treatment with diamond burs and instruments with metal tips resulted in an increase of roughness. Use of silicone polishers led to smoothening, which was more pronounced on the anatase surface. Plastic instruments, the air abrasive system and rubber cups left the surfaces intact. Functionality was partially lost on surfaces subjected to hand instruments and completely lost upon diamond burs and silicone polishers.'] Does peri-implantitis cleaning instrumentation influence the integrity of photoactive nanocoatings?