 To validate our  technique, we first use the statistics derived from the real time-lapse series of MT gliding assays to produce a large set of simulated data.
<clarity>  To validate our  technique, we first use the statistics derived from the real time-lapse series of MT gliding assays to produce a large set of simulated data.
 To validate our technique, we first use the statistics derived from the real time-lapse series of MT gliding assays to simulate realistic MT time-lapse image series in our simulated data.