Direct cDNA preamplification protocols developed for single-cell RNA-seq (scRNA-seq) have enabled transcriptome profiling of  rare cells without having to pool multiple samples or to perform RNA extraction.
<clarity> Direct cDNA preamplification protocols developed for single-cell RNA-seq (scRNA-seq) have enabled transcriptome profiling of  rare cells without having to pool multiple samples or to perform RNA extraction.
Direct cDNA preamplification protocols developed for single-cell RNA-seq (scRNA-seq) have enabled transcriptome profiling of rare cells without sample pooling or RNA extraction.