 In binary  DPPC:CHOL  mixtures, both cholesterol and DPPC acyl chains were observed spontaneously entering deep "non-annular" cavities in the nAChR TMD, particularly at the subunit interface and the \beta subunit center, facilitated by the low amino acid density in the cryo-EM structure of nAChR in a native membrane.
<fluency>  In binary  DPPC:CHOL  mixtures, both cholesterol and DPPC acyl chains were observed spontaneously entering deep "non-annular" cavities in the nAChR TMD, particularly at the subunit interface and the \beta subunit center, facilitated by the low amino acid density in the cryo-EM structure of nAChR in a native membrane.
 In binary DPPC:CHOL ) mixtures, both cholesterol and DPPC acyl chains were observed spontaneously entering deep "non-annular" cavities in the nAChR TMD, particularly at the subunit interface and the \beta subunit center, facilitated by the low amino acid density in the cryo-EM structure of nAChR in a native membrane.