Allysine quantification In one study, allysine is first reacted in acidic conditions (6M HCl, 110 °C, 24 h) with sodium 2-naphthol-6-sulfonate . A fluorescent bis-naphtol allysine is the product. Allysine is then quantified through use of high-performance liquid chromatography (HPLC). The results of this study provide a statistically relevant method in correlating greater concentrations of allysine and fibrotic tissue. The study shows that fibrotic tissue contains 2.5 times more alyssine than non-fibrotic tissue.
Allysine quantification Allysine can be reacted with sodium 2-naphthol-6-sulfonate to produce a fluorescent bis-naphtol allysine product. Allysine can then be quantified through use of high-performance liquid chromatography (HPLC). This method of detection was used show allysine has a 2.5 higher concentration in fibrotic vs. normal tissue.
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In one study, allysine is first reacted in acidic conditions (6M HCl, 110 °C, 24 h)
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The results of this study provide a statistically relevant method in correlating greater concentrations of allysine and fibrotic tissue. The study shows that fibrotic tissue contains
This method of detection was used show allysine has a
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times more alyssine than non-fibrotic
higher concentration in fibrotic vs. normal