Automatic analyses and comparisons of different stages of embryonic development largely depend on a highly accurate spatio-temporal alignment of the investigated data sets. In this contribution, we compare multiple approaches to perform automatic staging of developing embryos that were imaged with time-resolved 3D light-sheet microscopy. The methods comprise image-based convolutional neural networks as well as an approach based on the PointNet architecture that directly operates on 3D point clouds of detected cell nuclei centroids. The proof-of-concept experiments with four wild-type zebrafish embryos render both approaches suitable for automatic staging with average deviations of 0.45 - 0.57 hours .
Automatic analyses and comparisons of different stages of embryonic development largely depend on a highly accurate spatiotemporal alignment of the investigated data sets. In this contribution, we assess multiple approaches to perform automatic staging of developing embryos that were imaged with time-resolved 3D light-sheet microscopy. The methods comprise image-based convolutional neural networks as well as an approach based on the PointNet architecture that directly operates on 3D point clouds of detected cell nuclei centroids. The experiments with four wild-type zebrafish embryos render both approaches suitable for automatic staging with average deviations of 21 - 34 minutes. Moreover, a proof-of-concept evaluation based on simulated 3D+t point cloud data sets shows that average deviations of less than 7 minutes are possible .
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spatio-temporal
spatiotemporal
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compare
assess
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proof-of-concept
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0.45
21
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0.57 hours
34 minutes. Moreover, a proof-of-concept evaluation based on simulated 3D+t point cloud data sets shows that average deviations of less than 7 minutes are possible