Ca^{2+} plays  important role in cell signal transduction. Its intracellular propagation is the most basic process of Ca^{2+} signaling, such as calcium wave and double messenger system. In this wrok, the 3-dimensional probability distribution of Ca^{2+}  which is read out by phosphorylation  is studied in two scenarios with simulation and mean field ansatz . The coverage of distribution of Ca^{2+} is found at an order of magnitude of \mum, which is consistent with experimental observed calcium spike and wave. Our results suggest that the double messenger system may occur in the ER-PM junction to acquire great efficiency. The buffer effect of kinase is also discussed by calculating the average position of phosphorylations and free Ca^{2+} . The results in PKC scenario with two boundaries are found consistent with the results in CaM scenario especially at large distance as suggested by mean field ansatz . The results are helpful to understand the mechanism of Ca^{2+} signaling.
Ca^{2+} plays an important role in cell signal transduction. Its intracellular propagation is the most basic process of Ca^{2+} signaling, such as calcium wave and double messenger system. In this work, with both numerical simulation and mean field ansatz, the 3-dimensional probability distribution of Ca^{2+} , which is read out by phosphorylation , is studied in two scenarios with boundaries . The coverage of distribution of Ca^{2+} is found at an order of magnitude of \mum, which is consistent with experimental observed calcium spike and wave. Our results suggest that the double messenger system may occur in the ER-PM junction to acquire great efficiency. The buffer effect of kinase is also discussed by calculating the average position of phosphorylations and free Ca^{2+} . The results are helpful to understand the mechanism of Ca^{2+} signaling.
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wrok,
work, with both numerical simulation and mean field ansatz,
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simulation and mean field ansatz
boundaries
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. The results in PKC scenario with two boundaries are found consistent with the results in CaM scenario especially at large distance as suggested by mean field ansatz