Characteristics Although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates. In general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their characteristics are: Expression of Fc receptor and C3b receptors while lacking surface and cytoplasmic immunoglobulins. Production of IL-1. Positive detection of alpha-naphthyl butyrate esterase and lysozymes Phagocytic physiology (both for latex beads and sensitized erythrocytes). Restoration of the response of purified T lymphocytes to Con A. Increased CO2 production on phagocytosis and differentiation into macrophage-like cells Polarization into the M1 phenotype by incubation with IFN-γ and LPS, or to the M2 phenotype by incubation with interleukin 4 and interleukin 13 Differentiation into immature dendritic cells, using recombinant human interleukin 4 (rhIL-4) and recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF), and mature dendritic cells using rhIL-4, rhGM-CSF, recombinant human tumour necrotic factor α (rhTNF-α) and Ionomycin. The HLA type for THP-1 using molecular-based typing methods is HLA-A*02  ; B*15  ; C*03  ; DRB1*01 , DRB1*15  ; DRB5*01/ 02; DQB1*05, DQB1*06 .
Characteristics Although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates. In general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their characteristics are: Expression of Fc receptor and C3b receptors while lacking surface and cytoplasmic immunoglobulins. Production of IL-1. Positive detection of alpha-naphthyl butyrate esterase and lysozymes Phagocytic physiology (both for latex beads and sensitized erythrocytes). Restoration of the response of purified T lymphocytes to Con A. Increased CO2 production on phagocytosis and differentiation into macrophage-like cells Polarization into the M1 phenotype by incubation with IFN-γ and LPS, or to the M2 phenotype by incubation with interleukin 4 and interleukin 13 Differentiation into immature dendritic cells, using recombinant human interleukin 4 (rhIL-4) and recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF), and mature dendritic cells using rhIL-4, rhGM-CSF, recombinant human tumour necrotic factor α (rhTNF-α) and Ionomycin. The HLA type for THP-1 is HLA-A*02 :01; A*24:02 ; B*15 :11; B*35:01 ; C*03 :03 ; DRB1*01 :01; DRB1*15 :01; DQB1*05:01 ; DQB1*06: 02; DPB1*02:01; DPB1*04:02 (in the German Collection of Microorganisms and Cell Cultures (DSMZ) cell bank). This HLA type can change depending on the reference biorepository, due to loss of heterozygosity in various chromosomal regions, as THP-1 from the American Type Culture Collection (ATCC) do not express the HLA-A*24:02 and B*35:01 alleles .
D
using molecular-based typing methods
A
:01; A*24:02
A
:11; B*35:01
A
:03
R
,
:01;
A
:01; DQB1*05:01
R
DRB5*01/
DQB1*06:
R
DQB1*05, DQB1*06
DPB1*02:01; DPB1*04:02 (in the German Collection of Microorganisms and Cell Cultures (DSMZ) cell bank). This HLA type can change depending on the reference biorepository, due to loss of heterozygosity in various chromosomal regions, as THP-1 from the American Type Culture Collection (ATCC) do not express the HLA-A*24:02 and B*35:01 alleles