CAP-e (cell-based antioxidant protection in erythrocytes), is a novel in vitro bioassay for antioxidant activity developed by Alexander Schauss, Gitte Jensen, and associates at the American Institute for Biosocial and Medical Research (AIBMR), a private contract research organization (CRO) located in Puyallup, Washington, and Holger NIS, a private CRO located in Klamath Falls, Oregon. The CAP-e assay is performed by first incubating red blood cells (erythrocytes) with a test sample at a range of concentrations. The cells are then combined with dichloro fluorescein diacetate (DCFDA), which is oxidized in the presence of free radicals to form a green fluorescent byproduct (DCF). In the next step of the assay, exogenous hydrogen peroxide is added at a concentration of 167 mM to artificially induce severe oxidative stress. The antioxidant activity of varying concentrations of the test compound is measured based on the degree of inhibition of DCF-fluorescence, which is an indirect and nonspecific measure of reactive oxygen species production. To date, the assay has been used in 2 published studies, both conducted by AIBMR and Holger NIS.
CAP-e (cell-based antioxidant protection in erythrocytes), is a non-validated in vitro bioassay for antioxidant activity . The assay is performed by incubating red blood cells with a test sample at a range of concentrations. The cells are then combined with dichloro fluorescein diacetate (DCFDA), which is oxidized in the presence of free radicals to form a green fluorescent byproduct (DCF). Hydrogen peroxide is then added at to artificially induce severe oxidative stress. The antioxidant activity of varying concentrations of the test compound is measured based on the degree of inhibition of DCF-fluorescence, which is an indirect and nonspecific measure of reactive oxygen species production. To date, the assay has been used in 2 published studies, both conducted by AIBMR and Holger NIS.
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novel
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developed by Alexander Schauss, Gitte Jensen, and associates at the American Institute for Biosocial and Medical Research (AIBMR), a private contract research organization (CRO) located in Puyallup, Washington, and Holger NIS, a private CRO located in Klamath Falls, Oregon.
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The CAP-e
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(erythrocytes)
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In the next step of the assay, exogenous hydrogen peroxide is added at a concentration of 167 mM
Hydrogen peroxide is then added at