DNA based nanostructures built on a long single stranded DNA scaffold, known as DNA origamis, are nowadays the basis of many applications. These applications range from the control of single-molecule chemical reaction networks to URLanization at the nanometer scale of various molecules including proteins and carbon nanotubes. However, many basic questions concerning the mechanisms of formation of the origamis have not been addressed so far. For instance, the robustness of different designs against factors, such as the internal topology, or the influence of the staple pattern, are handled empirically. We have built a model for the folding and melting processes of DNA origamis that is able to reproduce accurately several thermodynamic quantities measurable from UV absorption experiments. The model can also be used to design a new distribution of crossovers that increases the robustness of the DNA template. The model provides predictions among which a few of them have been already successfully verified. Therefore, in spite of its complexity we propose an algorithm that gives the capability to design and fabricate templates with dedicated properties, a necessary step for technological development .
DNA based nanostructures built on a long single stranded DNA scaffold, known as DNA origamis, offer the possibility URLanize various molecules at the nanometer scale in one pot experiments. The folding of the scaffold is guaranteed by the presence of short, single stranded DNA sequences (staples), that hold together separate regions of the scaffold. In this paper, we modelize the annealing-melting properties of these DNA constructions. The model captures important features such as the hysteresis between melting and annealing, as well as the dependence upon the topology of the scaffold. We show that cooperativity between staples is critical to quantitatively explain the folding process of DNA origamis .
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are nowadays the basis of many applications. These applications range from the control of single-molecule chemical reaction networks to URLanization
offer the possibility URLanize various molecules
R
of various molecules including proteins and carbon nanotubes. However, many basic questions concerning the mechanisms of formation of the origamis have not been addressed so far. For instance, the robustness of different designs against factors,
in one pot experiments. The folding of the scaffold is guaranteed by the presence of short, single stranded DNA sequences (staples), that hold together separate regions of the scaffold. In this paper, we modelize the annealing-melting properties of these DNA constructions. The model captures important features
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internal topology, or the influence of the staple pattern, are handled empirically. We have built a model for the folding and melting processes of DNA origamis that is able to reproduce accurately several thermodynamic quantities measurable from UV absorption experiments. The model can also be used to design a new distribution of crossovers that increases the robustness of the DNA template. The model provides predictions among which a few of them have been already successfully verified. Therefore, in spite of its complexity we propose an algorithm that gives the capability to design and fabricate templates with dedicated properties, a necessary step for technological development
hysteresis between melting and annealing, as well as the dependence upon the topology of the scaffold. We show that cooperativity between staples is critical to quantitatively explain the folding process of DNA origamis