On 2018-01-17 two electron crystallography structures (with PDB entries 6AXZ, 6BTK) on a prion protofibril of bank vole PrP(168-176) (a segment in the PrP \beta2-\alpha2 loop) were released into the PDB Bank. The paper published by [Nat Struct Mol Biol 25(2):131-134 (2018)] reports some polar clasps for these two crystal structures, and "an intersheet hydrogen bond between Tyr169 and the backbone carbonyl of Asn171 on an opposing strand." However , by revisiting the polar clasps, we cannot confirm this very important intersheet hydrogen bond; instead we found another hydrogen bond ( Asn171 :H-Gln172: OE1  between the strand  of one sheet and the opposing strand  of the mating sheet ) to replace it .
On 2018-01-17 two electron crystallography structures (with PDB entries 6AXZ, 6BTK) on a prion protofibril of bank vole PrP(168-176) (a segment in the PrP \beta2-\alpha2 loop) were released into the PDB Bank. The paper published by [Nat Struct Mol Biol 25(2):131-134 (2018)] reports some polar clasps for these two crystal structures, and "an intersheet hydrogen bond between Tyr169 and the backbone carbonyl of Asn171 on an opposing strand." In addition , by revisiting the polar clasps, we found another two hydrogen bonds (B. Asn171 @H-A.Gln172 OE1 , B.Tyr169@OH-A.Gln172@N) between the strand A of one sheet and the opposing strand B of the mating sheet .
R
However
In addition
R
cannot confirm this very important intersheet hydrogen bond; instead we found another hydrogen bond (
found another two hydrogen bonds (B.
R
:H-Gln172:
@H-A.Gln172
A
, B.Tyr169@OH-A.Gln172@N)
A
A
A
B
D
) to replace it