In the method, an oligonucleotide primer hybridizes to a complementary region along the nucleic acid, to form a duplex, with the primer’s terminal 3’ end directly adjacent to the nucleotide base to be identified. The oligonucleotide primer is enzymatically extended  a single base by a nucleotide terminator complementary to the nucleotide being identified. The terminator prevents additional nucleotides from being incorporated . Many approaches can be taken for determining the identity of a terminator, including fluorescence labeling, mass labeling for mass spectrometry, measuring enzyme activity using a protein moiety, and isotope labeling. The method was invented by Phillip Goelet, Michael Knapp, Richard Douglas and Stephen Anderson while working at the company Molecular Tool  . The Illumina Methylation Assay utilizes this method in their Infinium technology to measure DNA methylation levels in the human genome.
In the method, an oligonucleotide primer hybridizes to a complementary region along the nucleic acid, to form a duplex, with the primer’s terminal 3’ end directly adjacent to the nucleotide base to be identified. The oligonucleotide primer is enzymatically extended by a single base in the presence of all four nucleotide terminators; the nucleotide terminator complementary to the base in the template being interrogated is incorporated and identified. The presence of all four terminators ensures that no further extension occurs beyond the single incorporated base . Many approaches can be taken for determining the identity of a terminator, including fluorescence labeling, mass labeling for mass spectrometry, measuring enzyme activity using a protein moiety, and isotope labeling. The method was invented by Philip Goelet, Michael Knapp, Richard Douglas and Stephen Anderson while working at the company Molecular Tool . This approach was designed for high-throughput single nucleotide polymorphism (SNP) genotyping and was originally called "Genetic Bit Analysis" (GBA) . The Illumina Methylation Assay utilizes this method in their Infinium technology to measure DNA methylation levels in the human genome.
A
by
R
by a nucleotide
in the presence of all four nucleotide terminators; the nucleotide
R
nucleotide being
base in the template being interrogated is incorporated and
R
terminator prevents additional nucleotides from being incorporated
presence of all four terminators ensures that no further extension occurs beyond the single incorporated base
R
Phillip
Philip
A
. This approach was designed for high-throughput single nucleotide polymorphism (SNP) genotyping and was originally called "Genetic Bit Analysis" (GBA)