Wastewater-based epidemiology (WBE) (or wastewater-based surveillance or sewage chemical-information mining) is a technique for determining the consumption of, or exposure to chemicals or pathogens in a population. This is achieved by measuring chemical or biological entities (biomarkers) in  wastewater generated by the people contributing to a wastewater treatment plant catchment. Wastewater-based epidemiology is commonly used to estimate illicit drug use in communities or populations, but can be used to measure the consumption of alcohol, caffeine, various pharmaceuticals and other compounds. Wastewater-based epidemiology has also been adapted to measure the load of pathogens such as SARS-CoV-2 in a community. It differs from traditional drug testing, urine or stool testing in that results are a population aggregate. Wastewater-based epidemiology is an interdisciplinary endeavour that draws on input from specialists such as wastewater treatment plant operators, analytical chemists and epidemiologists. History By the turn of the 21st century, numerous studies reported identified anthropogenic chemicals and human pathogens in wastewater and in surface waters receiving wastewater discharge. Eventually, some of these studies these were able to use measurements of chemicals and pathogens as proxies for chemical consumption or pathogen exposure. For example, an early wastewater-based epidemiology study published in 2005 measured cocaine and its metabolite benzoylecgognine in water samples from the River Po in Italy. These measurements were used to estimate cocaine consumption in communities discharging wastewater into the river. Since then, wastewater-based epidemiology has further developed and is used in multiple countries to measure the consumption of various chemicals. Wastewater-based epidemiology has been supported by government bodies such as the European Monitoring Centre for Drugs and Drug Addiction in Europe. Similar counterparts in other countries, such as the Australian Criminal Intelligence Commission in Australia and authorities in China use wastewater-based epidemiology to monitor drug use in their populations. Principle Wastewater-based epidemiology can be likened to urinalysis on a community scale. Small molecule compounds consumed by an individual can be excreted in the urine and/or feces in the form of the unchanged parent compound or a metabolite. In communities with reticulated sewers , this urine combines with other wastes including other individuals' urine as they travel to a  wastewater treatment plant. Wastewater is sampled at the inlet of a wastewater treatment plant, prior to water treatment. This is ideally done with autosampler devices which collect 24-hour flow or time composite samples. These samples contain biochemical information, or biomarkers from all the people contributing to a wastewater treatment plant catchment. Collected samples are sent to a laboratory, where analytical chemistry techniques (namely liquid chromatography-mass spectrometry) are used to quantify compounds of interest. These results can be expressed in per capita loads using information regarding the volume of wastewater which the sample represents. Results can be further expressed as per capita loads when given the population served by a wastewater treatment plant. The following equation summarises how per capita consumption of a chemical of interest (e.g. a drug) is determined: Virus surveillance Wastewater can also be tested for signatures of viruses excreted via feces, such as the enteroviruses poliovirus, aichivirus and coronavirus. Systematic wastewater surveillance programs for monitoring enteroviruses, namely poliovirus, was instituted as early as 1996 in Russia. Wastewater testing is recognised as an important tool for poliovirus surveillance by the WHO, especially in situations where mainstream surveillance methods are lacking, or where viral circulation or introduction is URL Wastewater-based epidemiology of viruses has the potential to inform on the presence of viral outbreaks when or where it is not suspected. A 2013 study of archived wastewater samples from the Netherlands found viral RNA of Aichivirus A in Dutch sewage samples dating back to 1987, two years prior to the first identification of Aichivirus A in Japan. During the COVID-19 pandemic, wastewater-based epidemiology using qPCR and/or RNA-Seq was used in various countries as a complementary method for assessing the load of COVID-19 in populations. Regular surveillance programs for monitoring SARS-Cov-2 in wastewater has been instituted in populations within countries such as Canada, China, the Netherlands, Singapore, Spain and the United States.
Wastewater-based epidemiology (WBE) (or wastewater-based surveillance or sewage chemical-information mining) is a technique for determining the consumption of, or exposure to chemicals or pathogens in a population. This is achieved by measuring chemical or biological entities (biomarkers) in municipal wastewater generated by the people contributing to a sewage treatment plant catchment. Wastewater-based epidemiology is commonly used to estimate illicit drug use in communities or populations, but can be used to measure the consumption of alcohol, caffeine, various pharmaceuticals and other compounds. Wastewater-based epidemiology has also been adapted to measure the load of pathogens such as SARS-CoV-2 in a community. It differs from traditional drug testing, urine or stool testing in that results are a population aggregate. Wastewater-based epidemiology is an interdisciplinary endeavour that draws on input from specialists such as wastewater treatment plant operators, analytical chemists and epidemiologists. History By the turn of the 21st century, numerous studies reported identified anthropogenic chemicals and human pathogens in sewage and in surface waters receiving wastewater discharge. Eventually, some of these studies these were able to use measurements of chemicals and pathogens as proxies for chemical consumption or pathogen exposure. For example, an early wastewater-based epidemiology study published in 2005 measured cocaine and its metabolite benzoylecgognine in water samples from the River Po in Italy. These measurements were used to estimate cocaine consumption in communities discharging wastewater into the river. Since then, wastewater-based epidemiology has further developed and is used in multiple countries to measure the consumption of various chemicals. Wastewater-based epidemiology has been supported by government bodies such as the European Monitoring Centre for Drugs and Drug Addiction in Europe. Similar counterparts in other countries, such as the Australian Criminal Intelligence Commission in Australia and authorities in China use wastewater-based epidemiology to monitor drug use in their populations. Principle Wastewater-based epidemiology can be likened to urinalysis on a community scale. Small molecule compounds consumed by an individual can be excreted in the urine and/or feces in the form of the unchanged parent compound or a metabolite. In communities with sewerage , this urine combines with other wastes including other individuals' urine as they travel to a municipal wastewater treatment plant. Wastewater is sampled at the inlet of a wastewater treatment plant, prior to water treatment. This is ideally done with autosampler devices which collect 24-hour flow or time composite samples. These samples contain biochemical information, or biomarkers from all the people contributing to a wastewater treatment plant catchment. Collected samples are sent to a laboratory, where analytical chemistry techniques (namely liquid chromatography-mass spectrometry) are used to quantify compounds of interest. These results can be expressed in per capita loads using information regarding the volume of wastewater which the sample represents. Results can be further expressed as per capita loads when given the population served by a wastewater treatment plant. The following equation summarises how per capita consumption of a chemical of interest (e.g. a drug) is determined: Virus surveillance Sewage can also be tested for signatures of viruses excreted via feces, such as the enteroviruses poliovirus, aichivirus and coronavirus. Systematic wastewater surveillance programs for monitoring enteroviruses, namely poliovirus, was instituted as early as 1996 in Russia. Wastewater testing is recognised as an important tool for poliovirus surveillance by the WHO, especially in situations where mainstream surveillance methods are lacking, or where viral circulation or introduction is URL Wastewater-based epidemiology of viruses has the potential to inform on the presence of viral outbreaks when or where it is not suspected. A 2013 study of archived wastewater samples from the Netherlands found viral RNA of Aichivirus A in Dutch sewage samples dating back to 1987, two years prior to the first identification of Aichivirus A in Japan. During the COVID-19 pandemic, wastewater-based epidemiology using qPCR and/or RNA-Seq was used in various countries as a complementary method for assessing the load of COVID-19 in populations. Regular surveillance programs for monitoring SARS-Cov-2 in wastewater has been instituted in populations within countries such as Canada, China, the Netherlands, Singapore, Spain and the United States.
A
municipal
R
wastewater
sewage
R
wastewater
sewage
R
reticulated sewers
sewerage
A
municipal
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Wastewater
Sewage