During asymmetric cell divisions, cortical dyneins generate forcesessential to position the spindle after polarity cues, prescribing daughter cells fate. In nematode zygote, cortical dynein pulls on microtubules transiently, raising the question of its targeting and dynamics. Tracking and fluorescence correlation spectroscopy revealed that in the cytoplasm, dynein spots displayed directed motions toward the cortex, localized at microtubule plus-ends through EBP-2 / EB but are not actively transported. Surprisingly CLIP-1/CLIP170 is not involved. ebp-2(0) slightly reduced spindle rocking, thus most cortical forcesremain suggesting a redundant mechanism . At the cortex, to relate dynein residency and forces generating, we tracked dynein and found two dynamically distinct populations. One of them would correspond to force generating events. Our experiments also indicated that an asymmetric (polarized) dynein-microbutule on-rate, causes the force imbalance positioning the spindle. GPR-1/2 increases the overall dynein density but also residency time (pulling processivity), although this latter was not polarized .
During asymmetric cell division, dynein generates forces, which position the spindle to reflect polarity and ensure correct daughter cell fates. The transient cortical localization of dynein raises the question of its targeting . We found that it accumulates at the microtubule plus-ends like in budding yeast, indirectly hitch-hiking on \text{EBP-2 / unbinding dynamics as \text{EBP-2 . At the cortex, dynein tracks can be classified as having either directed or diffusive-like motion. Diffusive-like tracks reveal force-generating dyneins. Their densities are higher on the posterior tip of the embryos, where \text{GPR-1/2 the force imbalance reflecting polarity, and supplements the regulation of mitotic progression via the non-polarized detachment rate .
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divisions, cortical dyneins generate forcesessential to
division, dynein generates forces, which
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after polarity cues, prescribing daughter cells fate. In nematode zygote, cortical dynein pulls on microtubules transiently, raising
to reflect polarity and ensure correct daughter cell fates. The transient cortical localization of dynein raises
R
and dynamics. Tracking and fluorescence correlation spectroscopy revealed that in the cytoplasm, dynein spots displayed directed motions toward the cortex, localized at
. We found that it accumulates at the
R
through EBP-2
like in budding yeast, indirectly hitch-hiking on \text{EBP-2
R
EB but are not actively transported. Surprisingly CLIP-1/CLIP170 is not involved. ebp-2(0) slightly reduced spindle rocking, thus most cortical forcesremain suggesting a redundant mechanism
unbinding dynamics as \text{EBP-2
R
to relate dynein residency and forces generating, we tracked dynein and found two dynamically distinct populations. One of them would correspond to force generating events. Our experiments also indicated that an asymmetric (polarized) dynein-microbutule on-rate, causes
dynein tracks can be classified as having either directed or diffusive-like motion. Diffusive-like tracks reveal force-generating dyneins. Their densities are higher on the posterior tip of the embryos, where \text{GPR-1/2
R
positioning the spindle. GPR-1/2 increases the overall dynein density but also residency time (pulling processivity), although this latter was not polarized
reflecting polarity, and supplements the regulation of mitotic progression via the non-polarized detachment rate