Protein: Shugoshin 2A (Shugoshin-2) (Shugoshin-like 2) (Q7TSY8)
Organism: Mus musculus (Mouse)
Function: Cooperates with PPP2CA to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Has a crucial role in protecting REC8 at centromeres from cleavage by separase. During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis. May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation. Essential for recruiting KIF2C to the inner centromere and for correcting defective kinetochore attachments. Involved in centromeric enrichment of AUKRB in prometaphase.
Subcellular location: Nucleus {ECO:0000250|UniProtKB:Q562F6}. Chromosome, centromere {ECO:0000269|PubMed:17205076, ECO:0000269|PubMed:18084284}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:17205076}. Note=Centromeric localization requires the presence of BUB1 and AUKRB (By similarity). During meiosis I, accumulates at centromeres during diplotene, and colocalizes differentially with the cohesin subunits RAD21 and REC8 at metaphase I centromeres (PubMed:17205076, PubMed:18084284). SGO2A and RAD21 change their relative distributions during telophase I when sister-kinetochore association is lost (PubMed:17205076). During meiosis II, it shows a striking tension-dependent redistribution within centromeres throughout chromosome congression during prometaphase II, as it does during mitosis (PubMed:17205076). {ECO:0000250|UniProtKB:Q562F6, ECO:0000269|PubMed:17205076, ECO:0000269|PubMed:18084284}.
Interaction partners:
- Meiotic recombination protein REC8 homolog
- Shugoshin 1
- Serine/threonine-protein kinase PLK1
- Borealin
- Cell division cycle protein 20 homolog
- Cohesin subunit SA-3
- Separin
- Structural maintenance of chromosomes protein 3
- Structural maintenance of chromosomes protein 1B
- Kinesin-like protein KIF2C
Sequence:
MEYPGIKVDTVTSGIQRRVKGRIAKTNLNVSLASKIKAKILNNSSIFKISLKHNNRALARALSKEKENSRRITTEKMQLQKEVEKLNFENTFLRLKLNTLNKKLVEIESHVSNDLLTAIEISSLSEFHQGSFLLSATKKQRNSKQCKPAHLPYARVLLTSENDDDDGADDKWQTKCNNRTISKTSPDSTSSVSRQPSSLHQCNLKAFPPKEDNQKTCGSGHLEHTSSVDILPNESHSDQSPKSSLSEMKTAPSPSLRREKLSHGNVTMRKKCVSSTPDILYVTDLDHQPTSSPGSNWNNEIHGHTNETSNNTQRNAECFLDLPSESSSEPDAKRMELVQKNTDSFHFQKTVYDAADMELTATDIGKIVAVSKSKKNQNKKKADCRKETFRKVKGASSDKKRESSKRECKDGSEVGAEEEADAARAERGAGVLDGRGDSEEPNCISSTEQPSQVNTQKKRTLQNSSDQENIQNTKRRQTYTTDEQEETNPFSRHSVKFLQDGKFDLCQKTLHHNLSKPSRQTFVIRKSEKDNLFPNQEDKDTISENLEVTNEFHIDDLSIEANENVCDHETQTMLDLKKSVSAQQNQTKINKTKQKINRRTKIISVMSQVYEDNDKDIHVLEKDNFPFHTQANKETTSGNLESSKEFESPLLFTRDNGSLRDCKTQNVLDLHKQIPDLYPDRNESQISKIPRQKVNRKTEVISGVKCFSNDQGVHCSEKDKSLLLQKDKDFPGTLKDLSEFDTPAFCNKDSAKSCDYKSEMLLGLKKHDPNMQPACQDDSKAGKKLRQKVNRKTEIISKITQIHENDRGSTHDSLNKKLCQKVNISKIISQMNQIYETINEDGNGFKSSIKDCEDIKSCDFGEINSNKKENYDPIQDPCTLVKKTKRKGSCKAGSSLAGAKNRCGLQLTDSSQVQSVPLDSGLRHHPNEADSGPGEQTNLPKMQKQSAGRSLGDAFSVSLGKEGSRPAKAVSKMTPKSKKRKLPLGCSPETHGTVEITPNTDLAKAVDSQQTEKENYLEKEKIAKRKPDFCTKVLKPLSETCSSNIKNSSLDSMCKSSLPLSISSRKTLMLEESSSLESTCIFQVGDAAHEKITTGTRNPHHRTQKSTPGSRTSLVLVDTSSVSDTNPANPENESEGQSSHPMRRKRQCVPLNLTEPSLRSKMRR