Protein: Zinc finger SWIM domain-containing protein 8 (Q3UHH1)
Organism: Mus musculus (Mouse)
Function: Substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex that promotes target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). The SCF-like E3 ubiquitin-protein ligase complex acts by catalyzing ubiquitination and subsequent degradation of AGO proteins (AGO1, AGO2, AGO3 and/or AGO4), thereby exposing miRNAs for degradation (By similarity). Specifically recognizes and binds AGO proteins when they are engaged with a TDMD target (By similarity). May also acts as a regulator of axon guidance: specifically recognizes misfolded ROBO3 and promotes its ubiquitination and subsequent degradation (By similarity).
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:24012004, ECO:0000269|PubMed:35989311}. Note=Translocates together with its substrate into stress granules (SGs) under proteostatic stress. {ECO:0000269|PubMed:35989311}.
Interaction partners:
- Cullin-3
- E3 ubiquitin-protein ligase RBX1
- E3 ubiquitin-protein ligase ARIH1
- Leucine-zipper-like transcriptional regulator 1
- Cullin-2
- GATOR1 complex protein DEPDC5
- Kelch-like protein 9
- Kelch-like protein 13
- Potassium channel tetramerisation domain containing 17
- BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3
Sequence:
MELMFAEWEDGERFSFEDSDRFEEDSLCSFISEAESLCQNWRGWRKQSAGPNSPTGGGGGGGSGGTRTRDGLVIPLVELSAKQVAFHIPFEVVEKVYPPVPEQLQLRIAFWSFPENEEDIRLYSCLANGSADEFQRGDQLFRMRAVKDPLQIGFHLSATVVPPQMVPPKGAYNVAVMFDRCRVTSCSCTCGAGAKWCTHVVALCLFRIHNASAVCLRAPVSESLSRLQRDQLQKFAQYLISELPQQILPTAQRLLDELLSSQSTAINTVCGAPDPTAGPSASDQSTWYLDESTLTDNIKKTLHKFCGPSPVVFSDVNSMYLSSTEPPAAAEWACLLRPLRGREPEGVWNLLSIVREMFKRRDSNAAPLLEILTDQCLTYEQITGWWYSVRTSASHSSASGHTGRSNGQSEVAAHACASMCDEMVTLWRLAVLDPALSPQRRRELCAQLRQWQLKVIENVKRGQHKKTLERLFPGFRPAVEACYFNWEEAYPLPGVTYSGTDRKLALCWARALPARPGASRSGGLEESRPRPLPTEPAVRPKEPGAKRKGLGEGISSQRGPRRLSAEGGDKALHKMGPSGGKAKVLGGTGSGGKSSAGSGSKRRLSSEDSSLEPDLAEMSLDDSSLALGAEASTFGGFPESPPPCPSSVGSRGPSTFLPEPPDTYEEDAGVYFSEGPEPPTASADHPGLLPGEVCTRDDLPSTDDSGSGLHKTKEAAPAVGEEDDDYQAYYLNAQDGAGGEEEKAEGGTGEEHDLFAGLKPLEQESRMEVLFACAEALHAHGYSNEASRLTVELAQDLLANPPDLKVEPPPAKGKKNKVSTSRQTWVATNTLTKAAFLLTVLSERPEHHSLAFRVGMFALELQRPPASTKALEVKLAYQESEVAALLKKIPRGPSEMSTIRCRAEELREGTLCDYRPVLPLMLASFIFDVLCAPVVSLTGSRPPSRNWTNEMPGDEELGFEAAVAALGMKTTVSEAEHPLLCEGTRREKGDLALALMITYKDDQAKLKKILDKLLDRESQTHKPQTLSSFYSSSRPATANQRSPSKHGAPSAPGALQPLTSSSAGPAQPGNVAGAGPGPTEGFTEKNVPESSPHSPCEGLPPEAALTPRPEGKVPSRLALGSRGGYNGRGWGSPGRPKKKHTGMASIDSSAPETTSDSSPTLSRRPLRGGWAPTSWGRGQDSDSISSSSSDSLGSSSSSGSRRASASGGARAKTVDVGRCYKGRRPESHAPHVPNQPSEAAAHFYFELAKTVLIKAGGNSSTSIFTHPSSSGGHQGPHRNLHLCAFEIGLYALGLHNFVSPNWLSRTYSSHVSWITGQAMEIGSAALTILVECWDGHLTPPEVASLADRASRARDSNMVRAAAELALSCLPHAHALNPNEIQRALVQCKEQDNLMLEKACMAVEEAAKGGGVYPEVLFEVAHQWFWLYEETAGGSSTAREGATSCSGSGMRAAGEAGRGLPEGRGAPGTEPVTVAAAAVTAAATVVPVISVGSSLYPGPGLGHGHSPGLHPYTALQPHLPCSPQYLTHPAHPAHPMPHMPRPAVFPVPSSAYPQGVHPAFLGAQYPYSVTPPSLAATAVSFPVPSMAPITVHPYHTEPGLPLPTSVALSSVHPASTFPAIQGASLPALTTQPSPLVSGGFPPPEEETHSQPVNPHSLHHLHAAYRVGMLALEMLGRRAHNDHPNNFSRSPPYTDDVKWLLGLAAKLGVNYVHQFCVGAAKGVLSPFVLQEIVMETLQRLNPIHAHNHLRAPAFHQLVQRCQQAYMQYIHHRLIHLTPADYDDFVNAIRSARSAFCLTPMGMMQFNDILQNLKRSKQTKELWQRVSLEITTFSP