Protein: Ras-related protein rab-6.1 (P34213)
Organism: Caenorhabditis elegans
Function: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. In its active GTP-bound form, acts redundantly with rab-6.2 (in its active GTP-bound form) to positively regulate the retrograde trafficking of cargo molecules from endosomes to Golgi structures. Required for the retrograde trafficking of glr-1, a subunit of AMPA-type glutamate receptors (AMPRs), out of early endosomes and into the Golgi compartment in neurons. Together with rab-6.2, promotes the retrograde trafficking of mig-14 from endosomes to Golgi structures in the intestine. In oocytes, in its active GTP-bound form, involved in the membrane fusion and exocytosis of secretory vesicles (cortical granules) to play a role in the remodeling of the embryo surface following fertilization. Recruits sep-1 to cortical granules (derived from the Golgi complex) for exocytosis during the oocyte-to-embryo transition. Required for seam cell division and alae formation. Promotes spontaneous reversals in locomotion.
Subcellular location: Cell membrane {ECO:0000269|PubMed:22992455}; Lipid-anchor {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Cell projection, dendrite {ECO:0000269|PubMed:26891225}. Perikaryon {ECO:0000269|PubMed:26891225}. Golgi apparatus {ECO:0000269|PubMed:21613545, ECO:0000269|PubMed:26891225}. Cytoplasmic vesicle, secretory vesicle {ECO:0000269|PubMed:22992455}. Note=Following fertilization, localizes to cav-1-positive vesicles tethered to the plasma membrane (PubMed:22992455). Localization becomes dispersed throughout the cytoplasm once the vesicles fuse with the plasma membrane during meiotic anaphase I (PubMed:22992455). Temporarily co-localizes with sep-1 in the cortical granules prior to membrane fusion (PubMed:22992455). Co-localizes with rab-11.1-positive vesicles near the plasma membrane until vesicle exocytosis in embryos (PubMed:22992455). Co-localizes with rab-6.2 at vesicular structures throughout the oocyte cytoplasm (PubMed:22992455). Co-localizes with rab-6.2 in neuronal cell bodies and dendrites and in Golgi structures in neurons and the intestine (PubMed:26891225). Co-localizes with vps-52 at Golgi structures (PubMed:21613545). {ECO:0000269|PubMed:21613545, ECO:0000269|PubMed:22992455, ECO:0000269|PubMed:26891225}.
Interaction partners:
- Rab GDP dissociation inhibitor
- Ras-related protein rab-6.2
- Protein bicaudal D homolog
- Vacuolar protein sorting-associated protein 39 homolog
- Rab GTPase-activating protein tbc-11
- Guanine nucleotide exchange factor subunit R06F6.8
- Vacuolar protein sorting-associated protein 33B
- Vacuolar protein sorting-associated protein 33A
- Vacuolar protein sorting-associated protein 52 homolog
- Conserved oligomeric Golgi complex subunit 6
Sequence:
MADFTNNALKKFKLVFLGEQSVGKTSIITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTIRLQLWDTAGQERFRSLIPSYIRDSSVAVVVYDITNANSFHQTTKWVDDVRNERGCDVIIVLVGNKTDLADKRQVSTEDGEKKARDLNVMFIETSAKAGYNVKQLFRKIATALPGIVQEETPEQPNIVIMNPPKDAEESQGRQCPC