Protein: Pikromycin polyketide synthase component PikAI (Pikromycin PKS component PikAI) (EC 2.3.1.239) (EC 2.3.1.240) (Narbonolide/10-deoxymethynolide synthase PikA1, modules 1 and 2) (Narbonolide/10-deoxymethynolide synthase PikAI) (Type I modular polyketide synthase PikAI) (PKS) (Q9ZGI5)
Organism: Streptomyces venezuelae
Function: Involved in the biosynthesis of 12- and 14-membered ring macrolactone antibiotics such as methymycin and neomethymycin, and pikromycin and narbomycin, respectively. Component of the pikromycin PKS which catalyzes the biosynthesis of both precursors 10-deoxymethynolide (12-membered ring macrolactone) and narbonolide (14-membered ring macrolactone). Chain elongation through PikAI, PikAII and PikAIII followed by thioesterase catalyzed termination results in the production of 10-deoxymethynolide, while continued elongation through PikAIV, followed by thioesterase (TE) catalyzed cyclization results in the biosynthesis of the narbonolide.
Interaction partners:
Sequence:
MSSAGITRTGARTPVTGRGAAAWDTGEVRVRRGLPPAGPDHAEHSFSRAPTGDVRAELIRGEMSTVSKSESEEFVSVSNDAGSAHGTAEPVAVVGISCRVPGARDPREFWELLAAGGQAVTDVPADRWNAGDFYDPDRSAPGRSNSRWGGFIEDVDRFDAAFFGISPREAAEMDPQQRLALELGWEALERAGIDPSSLTGTRTGVFAGAIWDDYATLKHRQGGAAITPHTVTGLHRGIIANRLSYTLGLRGPSMVVDSGQSSSLVAVHLACESLRRGESELALAGGVSLNLVPDSIIGASKFGGLSPDGRAYTFDARANGYVRGEGGGFVVLKRLSRAVADGDPVLAVIRGSAVNNGGAAQGMTTPDAQAQEAVLREAHERAGTAPADVRYVELHGTGTPVGDPIEAAALGAALGTGRPAGQPLLVGSVKTNIGHLEGAAGIAGLIKAVLAVRGRALPASLNYETPNPAIPFEELNLRVNTEYLPWEPEHDGQRMVVGVSSFGMGGTNAHVVLEEAPGGCRGASVVESTVGGSAVGGGVVPWVVSAKSAAALDAQIERLAAFASRDRTDGVDAGAVDAGAVDAGAVARVLAGGRAQFEHRAVVVGSGPDDLAAALAAPEGLVRGVASGVGRVAFVFPGQGTQWAGMGAELLDSSAVFAAAMAECEAALSPYVDWSLEAVVRQAPGAPTLERVDVVQPVTFAVMVSLARVWQHHGVTPQAVVGHSQGEIAAAYVAGALSLDDAARVVTLRSKSIAAHLAGKGGMLSLALSEDAVLERLAGFDGLSVAAVNGPTATVVSGDPVQIEELARACEADGVRARVIPVDYASHSRQVEIIESELAEVLAGLSPQAPRVPFFSTLEGAWITEPVLDGGYWYRNLRHRVGFAPAVETLATDEGFTHFVEVSAHPVLTMALPGTVTGLATLRRDNGGQDRLVASLAEAWANGLAVDWSPLLPSATGHHSDLPTYAFQTERHWLGEIEALAPAGEPAVQPAVLRTEAAEPAELDRDEQLRVILDKVRAQTAQVLGYATGGQIEVDRTFREAGCTSLTGVDLRNRINAAFGVRMAPSMIFDFPTPEALAEQLLLVVHGEAAANPAGAEPAPVAAAGAVDEPVAIVGMACRLPGGVASPEDLWRLVAGGGDAISEFPQDRGWDVEGLYHPDPEHPGTSYVRQGGFIENVAGFDAAFFGISPREALAMDPQQRLLLETSWEAVEDAGIDPTSLRGRQVGVFTGAMTHEYGPSLRDGGEGLDGYLLTGNTASVMSGRVSYTLGLEGPALTVDTACSSSLVALHLAVQALRKGEVDMALAGGVAVMPTPGMFVEFSRQRGLAGDGRSKAFAASADGTSWSEGVGVLLVERLSDARRNGHQVLAVVRGSALNQDGASNGLTAPNGPSQQRVIRRALADARLTTSDVDVVEAHGTGTRLGDPIEAQALIATYGQGRDDEQPLRLGSLKSNIGHTQAAAGVSGVIKMVQAMRHGLLPKTLHVDEPSDQIDWSAGAVELLTEAVDWPEKQDGGLRRAAVSSFGISGTNAHVVLEEAPVVVEGASVVEPSVGGSAVGGGVTPWVVSAKSAAALDAQIERLAAFASRDRTDDADAGAVDAGAVAHVLADGRAQFEHRAVALGAGADDLVQALADPDGLIRGTASGVGRVAFVFPGQGTQWAGMGAELLDSSAVFAAAMAECEAALSPYVDWSLEAVVRQAPGAPTLERVDVVQPVTFAVMVSLARVWQHHGVTPQAVVGHSQGEIAAAYVAGALPLDDAARVVTLRSKSIAAHLAGKGGMLSLALNEDAVLERLSDFDGLSVAAVNGPTATVVSGDPVQIEELAQACKADGFRARIIPVDYASHSRQVEIIESELAQVLAGLSPQAPRVPFFSTLEGTWITEPVLDGTYWYRNLRHRVGFAPAIETLAVDEGFTHFVEVSAHPVLTMTLPETVTGLGTLRREQGGQERLVTSLAEAWVNGLPVAWTSLLPATASRPGLPTYAFQAERYWLENTPAALATGDDWRYRIDWKRLPAAEGSERTGLSGRWLAVTPEDHSAQAAAVLTALVDAGAKVEVLTAGADDDREALAARLTALTTGDGFTGVVSLLDGLVPQVAWVQALGDAGIKAPLWSVTQGAVSVGRLDTPADPDRAMLWGLGRVVALEHPERWAGLVDLPAQPDAAALAHLVTALSGATGEDQIAIRTTGLHARRLARAPLHGRRPTRDWQPHGTVLITGGTGALGSHAARWMAHHGAEHLLLVSRSGEQAPGATQLTAELTASGARVTIAACDVADPHAMRTLLDAIPAETPLTAVVHTAGALDDGIVDTLTAEQVRRAHRAKAVGASVLDELTRDLDLDAFVLFSSVSSTLGIPGQGNYAPHNAYLDALAARRRATGRSAVSVAWGPWDGGGMAAGDGVAERLRNHGVPGMDPELALAALESALGRDETAITVADIDWDRFYLAYSSGRPQPLVEELPEVRRIIDARDSATSGQGGSSAQGANPLAERLAAAAPGERTEILLGLVRAQAAAVLRMRSPEDVAADRAFKDIGFDSLAGVELRNRLTRATGLQLPATLVFDHPTPLALVSLLRSEFLGDEETADARRSAALPATVGAGAGAGAGTDADDDPIAIVAMSCRYPGDIRSPEDLWRMLSEGGEGITPFPTDRGWDLDGLYDADPDALGRAYVREGGFLHDAAEFDAEFFGVSPREALAMDPQQRMLLTTSWEAFERAGIEPASLRGSSTGVFIGLSYQDYAARVPNAPRGVEGYLLTGSTPSVASGRIAYTFGLEGPATTVDTACSSSLTALHLAVRALRSGECTMALAGGVAMMATPHMFVEFSRQRALAPDGRSKAFSADADGFGAAEGVGLLLVERLSDARRNGHPVLAVVRGTAVNQDGASNGLTAPNGPSQQRVIRQALADARLAPGDIDAVETHGTGTSLGDPIEAQGLQATYGKERPAERPLAIGSVKSNIGHTQAAAGAAGIIKMVLAMRHGTLPKTLHADEPSPHVDWANSGLALVTEPIDWPAGTGPRRAAVSSFGISGTNAHVVLEQAPDAAGEVLGADEVPEVSETVAMAGTAGTSEVAEGSEASEAPAAPGSREASLPGHLPWVLSAKDEQSLRGQAAALHAWLSEPAADLSDADGPARLRDVGYTLATSRTAFAHRAAVTAADRDGFLDGLATLAQGGTSAHVHLDTARDGTTAFLFTGQGSQRPGAGRELYDRHPVFARALDEICAHLDGHLELPLLDVMFAAEGSAEAALLDETRYTQCALFALEVALFRLVESWGMRPAALLGHSVGEIAAAHVAGVFSLADAARLVAARGRLMQELPAGGAMLAVQAAEDEIRVWLETEERYAGRLDVAAVNGPEAAVLSGDADAAREAEAYWSGLGRRTRALRVSHAFHSAHMDGMLDGFRAVLETVEFRRPSLTVVSNVTGLAAGPDDLCDPEYWVRHVRGTVRFLDGVRVLRDLGVRTCLELGPDGVLTAMAADGLADTPADSAAGSPVGSPAGSPADSAAGALRPRPLLVALLRRKRSETETVADALGRAHAHGTGPDWHAWFAGSGAHRVDLPTYSFRRDRYWLDAPAADTAVDTAGLGLGTADHPLLGAVVSLPDRDGLLLTGRLSLRTHPWLADHAVLGSVLLPGAAMVELAAHAAESAGLRDVRELTLLEPLVLPEHGGVELRVTVGAPAGEPGGESAGDGARPVSLHSRLADAPAGTAWSCHATGLLATDRPELPVAPDRAAMWPPQGAEEVPLDGLYERLDGNGLAFGPLFQGLNAVWRYEGEVFADIALPATTNATAPATANGGGSAAAAPYGIHPALLDASLHAIAVGGLVDEPELVRVPFHWSGVTVHAAGAAAARVRLASAGTDAVSLSLTDGEGRPLVSVERLTLRPVTADQAAASRVGGLMHRVAWRPYALASSGEQDPHATSYGPTAVLGKDELKVAAALESAGVEVGLYPDLAALSQDVAAGAPAPRTVLAPLPAGPADGGAEGVRGTVARTLELLQAWLADEHLAGTRLLLVTRGAVRDPEGSGADDGGEDLSHAAAWGLVRTAQTENPGRFGLLDLADDASSYRTLPSVLSDAGLRDEPQLALHDGTIRLARLASVRPETGTAAPALAPEGTVLLTGGTGGLGGLVARHVVGEWGVRRLLLVSRRGTDAPGADELVHELEALGADVSVAACDVADREALTAVLDAIPAEHPLTAVVHTAGVLSDGTLPSMTTEDVEHVLRPKVDAAFLLDELTSTPAYDLAAFVMFSSAAAVFGGAGQGAYAAANATLDALAWRRRAAGLPALSLGWGLWAETSGMTGELGQADLRRMSRAGIGGISDAEGIALLDAALRDDRHPVLLPLRLDAAGLRDAAGNDPAGIPALFRDVVGARTVRARPSAASASTTAGTAGTPGTADGAAETAAVTLADRAATVDGPARQRLLLEFVVGEVAEVLGHARGHRIDAERGFLDLGFDSLTAVELRNRLNSAGGLALPATLVFDHPSPAALASHLDAELPRGASDQDGAGNRNGNENGTTASRSTAETDALLAQLTRLEGALVLTGLSDAPGSEEVLEHLRSLRSMVTGETGTGTASGAPDGAGSGAEDRPWAAGDGAGGGSEDGAGVPDFMNASAEELFGLLDQDPSTD