Protein: E3 ubiquitin-protein ligase PRT6 (EC 2.3.2.27) (Protein GREENING AFTER EXTENDED DARKNESS 1) (Protein PROTEOLYSIS 6) (RING-type E3 ubiquitin transferase PRT6) (F4KCC2)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Ubiquitin protein ligase which is a component of the N-end rule pathway with arginine specificity, and functions with the arginyltransferases ATE1 and ATE2. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Does not participate in degradation of proteins with N-terminal Phe or Leu. The N-end rule pathway regulates seed after-ripening, seedling sugar sensitivity, seedling lipid breakdown, and abscisic acid (ABA) sensitivity of germination. The N-end rule pathway regulates various aspects of leaf and shoot development. Involved in the ubiquitination and subsequent degradation of RAP2-12, an activator of hypoxic gene expression. The ubiquitination occurs after the N-arginylation of RAP2-12 by ATE1 or ATE2 under aerobic conditions. The end-rule pathway plays a role in regulating the timing and amplitude of the immune response following infection with the bacterial pathogen Pseudomonas syringae pv tomato. Regulates the biosynthesis of plant-defense metabolites such as glucosinolates, and the biosynthesis and response to the phytohormone jasmonate (JA), which plays a key role in plant immunity. Controls the expression of specific defense-response genes, activates the synthesis pathway for the phytoalexin camalexin, and influences basal resistance to the hemibiotroph pathogen Pseudomonas syringae pv tomato. Coordinates the mobilization of seed storage reserves and regulates the abundance and activities of several proteases following seed germination.
Interaction partners:
- E3 ubiquitin-protein ligase UPL3
- E3 ubiquitin-protein ligase UPL4
- Ubiquitin-conjugating enzyme E2 2
- Ubiquitin-conjugating enzyme E2 3
- Ubiquitin-conjugating enzyme E2 1
- Arginyl-tRNA--protein transferase 2
- BTB/POZ domain-containing protein At2g30600
- Arginyl-tRNA--protein transferase 1
- E3 ubiquitin-protein ligase PRT1
- Plant cysteine oxidase 1
Sequence:
METNSSLFGLVSPSSHDLVIERLASVGVPKKYRSKRGLVEFVRANPAKISELVSALLPTDDDVKLGLKEARERPRKSAVSPTMKKRFRESMNMLQWLMFQDEPDVSLRNLAKLNLDQRGVCGSVWGQNDIAYRCRTCENDPTCAICVPCFQNGDHNSHDYSIIYTGGGCCDCGDETAWKPDGFCSNHKGSEQIRPLSENLANSVGPILDALFTCWNNKLLSAESSGQKGARSNDTLVILQKMSNELTFIVVEMLLEFSMSSESLLSFVSRRIISSSGLLSILLKAERFLDQDVMKKLHDLFLKLIGDPVFKCEFAKAFVSYYPVVISEVVKQGTDNAFKKYPLLSTFSVQILTVPTLTPFLVKEMNLLAMLLGCLSDIFVSCSGEDGLLQATKLERLCETSERVIGDLKFVMSHAIVSKYATHEHRELSRSWLTLLTFAQGMNPLKRETGIPIDEENDYMHLFFVLGHSIAVIHSLLVNGTYSAASDEEIENDRNAKEEFDKCDGDGERYAKVGRLSHEDSVCTAIVSSSSFDSSMASEVHKIDPFHALLPSSAIYLIRECLKVLETCLGNDEGISKFLCKLSSSSGRNIPESKMSWPRRDLLNVETGGSVSSNLASSSRDPSTGLSPLCGDIQTNLSLDNVCGPYGVVQTDVTADSKRVSCNSADLTKNASGLRILGLCDWPDIHYDVSSQAISVHLPLHRLLSLLIQKALRICYGESASYNGVSISHEIPHADFFSSVIGDFHPCGFSALVMEHVLQIRVFCAQVIAGMWKKNGDSALVSCEWYRSVRWSEQGLELDLFLLQCCAALAPADSYVDKLLSRFGLSSYLSLNPDITNEYEPVLVQEMLGLLIQILQERRFCGLSTAESLRREIIFKLATGDFTHSQLVKSLPRDLSKSDELQEVLDDVSVYCNPSGMNQGKYSLQSSCWKELDLYHPRWQSRDLQSAEERFSRYCGVSALTTQLPRWRMIYPPLKGLARIGTCKATFQIISSALYYALQSGTSVKSRAPDGVLITALQLLSLSLDICTQQRQSNSQDCCLENSIPILELAGLEIIGIAQGTEKESLLSLLVSLMKTRMGDGRHQFPEPGSCNISSWIGNLLKKFSAIDSVCMNLLQSLAPEVVGQSGFDKVMSGSTSDEKRKAKAKERQAAIMAKMKAEQSKFLSTLSSSMDDDDPRSEFETSDSVMEHDSEIAVREVCSLCHDPDSKDPVSFLIFLQKSKLLSFVDRGPPSWDQCPQSEKKISVDGAPDLLRMNASSDSLRISSPLMLQLSDDTISESANMIESIKARLIGNGQTEKRSSDGRGKDESNMESLEIAMYQTVRNKIENMINQSLTRVDHQPHEAENCSEKNSVGGPSTLQGRFPDIRSRQTSRRPDAGSDGFHPIDCDGVYLSSCGHAVHQSCLERYLKSLKERSGRRTVFEGAHIVDLKKKEFLCPVCRRLANSVLPECPGDLCSVSKLQDSPRTKLRRKDALQPSLWLSEALCLLRSAAEVIEDGDRGKTVTPQGDGPRRKDLKSVSKMLWDFYFPKPEDKTLKRLWLPPQSIVMWDTLKYSLISMEIGTRFAKNSMLPVYCIDSLYEELKTSKGTILSVLLRVVQSSRTKNTIHVRQRFVGMKHLAESICYGVSSSSSSSIFGSEGTTGSLKNIDLLWNRASDPVLAHDPFSSLMWALFCLPFPFLTCEESLLSLVHIFHSVSLVQTVIAYCACRPSELSELNFGENLLNDISNALRESGGWEYFRSNNMDLSCDIKDTIRKYSLPFLRRCALLWKLLKSTPRKLHEESDMFDLPSDPTTDNMDFIYSPQSELNHVQELEKMFNIPPIDIILNDELLRSSTQIWLQHFQREYRVNRVKRSLCITPVVPFQLMKLPNLYQDLLQRCIKKRCVNCTKVIEEPVLCLLCGSLCSPIWSPCCRESGCPNHAITCGAGTGVFLLIRRTTILLQRFARQSPWPSPYLDTFGEEDIDMIRGKRLYLNEERYAALTYLVGSHGLDRSSKVLGQTTIGAVLH