Protein: RQC trigger complex helicase SLH1 (EC 3.6.4.13) (Antiviral helicase SLH1) (SKI2-like helicase 1) (P53327)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Involved in activation of the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation. Drives the splitting of stalled ribosomes that are polyubiquitinated in a HEL2-dependent manner, as part of the ribosome quality control trigger (RQT) complex. Also represses the translation of non-poly(A) mRNAs together with SKI2. May block translation by inhibiting translation initiation factor 5B (FUN12) action on mRNAs lacking a 3' poly(A) structure. Involved in antiviral defense, preventing L-A dsRNA virus propagation by specifically blocking translation of viral mRNAs.
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:14562095}.
Interaction partners:
- RQC trigger complex subunit CUE3
- RQC trigger complex subunit RQT4
- E3 ubiquitin-protein ligase HEL2
- Pre-mRNA-splicing factor 8
- Pre-mRNA-splicing factor 6
- Pre-mRNA-splicing factor CEF1
- Pre-mRNA-processing factor 17
- Pre-mRNA-processing protein 45
- Pre-mRNA-processing factor 19
- Pre-mRNA-splicing factor SYF1
Sequence:
MSTEYSADSSKSFMIAMQSMIDTSQTFNLDRSKISLPDFDDELKKVQKDEQNQRTELTVLSQDRNDWDDIFEEFKDISFAQLQSIIDSYKTKNAVAVYKKIGKLINEAETTLSSNVLLETVLQMVYKHQKQELEKELLDFLGTGNIDLVSLLLQHRRMIVATPIETTILLIKNAVNSTPEFLTQQDIRNQVLKSAEDAKNRKLNPATKIIKYPHVFRKYEAGSTTAMAFAGQKFTLPVGTTRMSYNTHEEIIIPAADQASNKNYLYTKLLKISDLDHFCKTVFPYETLNQIQSLVYPVAYKTNENMLICAPTGAGKTDIALLTIINTIKQFSVVNGENEIDIQYDDFKVIYVAPLKALAAEIVDKFSKKLAPFNIQVRELTGDMQLTKAEILATQVIVTTPEKWDVVTRKANGDNDLVSKVKLLIIDEVHLLHEDRGSVIETLVARTLRQVESSQSMIRIIGLSATLPNFMDVADFLGVNRQIGMFYFDQSFRPKPLEQQLLGCRGKAGSRQSKENIDKVAYDKLSEMIQRGYQVMVFVHSRKETVKSARNFIKLAESNHEVDLFAPDPIEKDKYSRSLVKNRDKDMKEIFQFGFGIHHAGMARSDRNLTEKMFKDGAIKVLCCTATLAWGVNLPADCVIIKGTQVYDSKKGGFIDLGISDVIQIFGRGGRPGFGSANGTGILCTSNDRLDHYVSLITQQHPIESRFGSKLVDNLNAEISLGSVTNVDEAIEWLGYTYMFVRMRKNPFTYGIDWEEIANDPQLYERRRKMIVVAARRLHALQMIVFDEVSMHFIAKDLGRVSSDFYLLNESVEIFNQMCDPRATEADVLSMISMSSEFDGIKFREEESKELKRLSDESVECQIGSQLDTPQGKANVLLQAYISQTRIFDSALSSDSNYVAQNSVRICRALFLIGVNRRWGKFSNVMLNICKSIEKRLWAFDHPLCQFDLPENIIRRIRDTKPSMEHLLELEADELGELVHNKKAGSRLYKILSRFPKINIEAEIFPITTNVMRIHIALGPDFVWDSRIHGDAQFFWVFVEESDKSQILHFEKFILNRRQLNNQHEMDFMIPLSDPLPPQVVVKVVSDTWIGCESTHAISFQHLIRPFNETLQTKLLKLRPLPTSALQNPLIESIYPFKYFNPMQTMTFYTLYNTNENAFVGSPTGSGKTIVAELAIWHAFKTFPGKKIVYIAPMKALVRERVDDWRKKITPVTGDKVVELTGDSLPDPKDVHDATIVITTPEKFDGISRNWQTRKFVQDVSLIIMDEIHLLASDRGPILEMIVSRMNYISSQTKQPVRLLGMSTAVSNAYDMAGWLGVKDHGLYNFPSSVRPVPLKMYIDGFPDNLAFCPLMKTMNKPVFMAIKQHSPDKPALIFVASRRQTRLTALDLIHLCGMEDNPRRFLNIDDEEELQYYLSQVTDDTLKLSLQFGIGLHHAGLVQKDRSISHQLFQKNKIQILIATSTLAWGVNLPAHLVIIKGTQFFDAKIEGYRDMDLTDILQMMGRAGRPAYDTTGTAIVYTKESKKMFYKHFLNVGFPVESSLHKVLDDHLGAEITSGSITNKQEALDFLSWTFLFRRAHHNPTYYGIEDDTSTAGVSEHLSSLIDSTLENLRESQCVLLHGDDIVATPFLSISSYYYISHLTIRQLLKQIHDHATFQEVLRWLSLAVEYNELPVRGGEIIMNEEMSQQSRYSVESTFTDEFELPMWDPHVKTFLLLQAHLSRVDLPIADYIQDTVSVLDQSLRILQAYIDVASELGYFHTVLTMIKMMQCIKQGYWYEDDPVSVLPGLQLRRIKDYTFSEQGFIEMTPQQKKKKLLTLEEIGRFGYKKLLNVFDQLTFGMTESEDTKKRFVSVCQRLPVLEGMKFEEQENNEVLTFYSKHLSSKHNNGFEVYCDKFPKIQKELWFLIGHKGDELLMIKRCQPKQMNKEVIIHCDLFIPEEIRGEELQFSLINDALGLRYDMVHKLIS