Protein: Activated Cdc42 kinase Ack (EC 2.7.10.2) (EC 2.7.11.1) (Q9VZI2)
Organism: Drosophila melanogaster (Fruit fly)
Function: Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in diverse biological functions such as cell survival, cell differentiation, cell growth and proliferation. Phosphorylates SH3PX1 and ex. Phosphorylates SH3PX1 predominantly on 'Tyr-56', which likely promotes the recruitment of SH3PX1 to an axonal guidance receptor complex that includes dock and Dscam; because phosphorylation of SH3PX1 increases its interaction with the complex member dock while decreasing its interaction with the actin cytoskeleton modulator WASp. In the wing and eye, promotes tissue growth, and during embryogenesis coordinates cell shape changes required for correct dorsal closure. Functions in the negative regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway by enhancing yki activity thereby promoting cell proliferation and inhibiting apoptosis. This is accomplished, at least in part, by phosphorylating ex thereby reducing its ability to efficiently activate the Hippo signaling cascade. In the eye disk, wing disk and possibly spermatids, inhibits programmed cell death induced by hid and rpr through a mechanism that is independent of the MAP kinase signal transduction pathway. Essential for male and female fertility. During oogenesis required for the correct temporal assembly, and consequently the catalytic activity of long CTPsyn filaments (cytoophidium) in the germline nurse cells, likely by phosphorylating an unidentified substrate that is essential for linking individual CTPsyn filaments into large, catalytically active assemblies.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:22615583, ECO:0000269|PubMed:25223282}. Cytoplasmic vesicle, clathrin-coated vesicle {ECO:0000269|PubMed:23562806}. Note=In nurse cells localizes to cytoophidium, a subcellular filamentary structure where CTP synthase (Ctps) is compartmentalized (PubMed:25223282). In third instar photoreceptor R-cells, expressed in the cytoplasm and cytoplasmic puncta (PubMed:22615583). In spermatocytes displays localization to peripheral clathrin-positive structures and localization to some secretory clathrin (PubMed:23562806). {ECO:0000269|PubMed:22615583, ECO:0000269|PubMed:23562806, ECO:0000269|PubMed:25223282}.
Interaction partners:
- Sorting nexin
- Dreadlocks, isoform B
- Growth factor receptor-bound protein 2
- Cdc42 homolog
Sequence:
MTSTSAVDGGLGSETAWLEDLLREVQLEQFLDRIRDDLQVTRLAHFDYVLPDDLERCGLGKPAIRRLMEAVRKKKAHQWRKNILSKLIGGGKQPSSKKQSSAARESSQGNGTQLTCLIHEKDITMGLKLGDGSFGVVRRGEWSASPAGKVIPVAVKVLKSDNLTQPGIIDDFFREVQAMHALDHANLVRLYGVVLSQPMMMITELAERGSLLDTLRKQCRHTSLTIIWNWSVQIVTGMAYLEQKRFLHRDLACRNVLLAAGNKIKIGDFGLMRALPQEDDCYVMSEHKKVPFPWCAPESLRFRQFSHASDTWMFGVTLWEMFSFGEDPWVGLNGSQILRKIDREGERLHQPDACPPDVYAMMLQCWDKTPAERPTFAALKEYLASMSPPVMRASRSHHESKGLQIEPGDTIAIIDGRHELKLIKGQNQRTFDIGIFPRNLLEQRKVGAAGDVVMRSSVGNGSSSSPFGFCWGGAAAMANGDDRQRKCASMTNQPHAKERKSTSSKQFAYNKLVNDSATGLQRRNAVKHKGVVVGPQRPPPPQFQQEGILIDISPDMRPIAEAGTGGAKGAGDSSSLQADSSFCILDAPIDVPTYAGSSGSGDLNVSPTYYNEQPQFDFDPAKMTASPGRLQPPPYQMPPTYSNTMEFVQKRDLHQQQLATPVRERDPFDTTNVETTVALYSNFNQSLEAASPPAPIYNSPSVRKSLFGGSKSNKENIPALESAAMQLNLSNLTLERHDATCIQPVEPVPAPPGDGVLLDKSFIAELEKDMYSNGQNRAQEEYQRNSTQMYASKDMVYKQNLTPLKNGAAPGSVHSNHSSPSSTASPKQNNVEAAAAAAATTQSVVNRIWYEQVASTQSEYYAQPPTEQAEEQIYQNHRHQQQQQQELNHSFVAISNRVVAPKNNAYSSTASLYDAVAASTAGSTYYGQVPNGSGAVLYDEVTQDDYLRPTRPAPLAPPPLSAQQIQRRMEKMRLQQQQQLDGAHQLYAPVPSDYGREQEKLQQLMQELGSSAVEQDVRNALRAASGDVGLATRHYKIDQLARLGVAGRPQCEQALQQTNWSLEVAAELLLNAG