Protein: Partitioning defective 3 homolog (PAR-3) (PARD-3) (Atypical PKC isotype-specific-interacting protein) (ASIP) (Atypical PKC-specific-binding protein) (ASBP) (Q9Z340)
Organism: Rattus norvegicus (Rat)
Function: Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly (By similarity). The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (By similarity). Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Involved in Schwann cell peripheral myelination. Targets the phosphatase PTEN to cell junctions.
Subcellular location: Cytoplasm {ECO:0000250}. Endomembrane system. Cell junction. Cell junction, tight junction {ECO:0000250|UniProtKB:Q99NH2}. Cell junction, adherens junction {ECO:0000250|UniProtKB:Q99NH2}. Cytoplasm, cell cortex {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Cell membrane {ECO:0000250}. Note=Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. Colocalized with SIRT2 in internode region of myelin sheath (By similarity). {ECO:0000250}.
Interaction partners:
- Partitioning defective 6 homolog alpha
- Protein kinase C zeta type
- Par-6 family cell polarity regulator beta
- Par-6 family cell polarity regulator gamma
- Protein kinase C iota type
- Cell division control protein 42 homolog
- Cadherin-5
- Junctional adhesion molecule A
- Nectin-1
- TIAM Rac1 associated GEF 1
Sequence:
MKVTVCFGRTRVVVPCGDGRMKVFSLIQQAVTRYRKAVAKDPNYWIQVHRLEHGDGGILDLDDILCDVADDKDRLVAVFDEQDPHHGGDGTSASSTGTQSPEIFGSELGTNNVSAFRPYQTTSEIEVTPSVLRANMPLHVRRSSDPALTGLSTSVSDNNFSSEEPSRKNPTRWSTTAGFLKQNTTGSPKTCDRKKDENYRSLPRDPSSWSNQFQRDNARSSLSASHPMVDRWLEKQEQDEEGTEEDSSRVEPVGHADTGLENMPNFSLDDMVKLVQVPNDGGPLGIHVVPFSARGGRTLGLLVKRLEKGGKAEQENLFHENDCIVRINDGDLRNRRFEQAQHMFRQAMRARVIWFHVVPAANKEQYEQLSQREMNNYSPGRFSPDSHCVANRSVANNAPQALPRAPRLSQPPEQLDAHPRLPHSAHASTKPPTAPALAPPNVLSTSVGSVYNTKRVGKRLNIQLKKGTEGLGFSITSRDVTIGGSAPIYVKNILPRGAAIQDGRLKAGDRLIEVNGVDLAGKSQEEVVSLLRSTKMEGTVSLLVFRQEEAFHPREMNAEPSQMQSPKETKAEDEDIVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKENHADLGIFVKSIINGGAASKDGRLRVNDQLIAVNGESLLGKANQEAMETLRRSMSTEGNKRGMIQLIVARRISRCNELRSPGSPAAPELPIETELDDRERRISHSLYSGIEGLDESPTRNAALSRIMGESGKCQLSPTVNMPHDDTVMIEDDRLPVLPPHLSDQSSSSSHDDVGFIMTEAGTWAKATISDSADCSLSPDVDPVLAFQREGFGRQSMSEKRTKQFSNASQLDFVKTRKSKSMDLGIADETKLNTVDDQRAGSPNRDVGPSLGLKKSSSLESLQTAVAEVTLNGNIPFHRPRPRIIRGRGCNESFRAAIDKSYDKPMVDDDDEGMETLEEDTEESSRSGRESVSTSSDQPSYSLERQMNGDPEKRDKAEKKKDKAGKDKKKDREKEKDKLKAKKGMLKGLGDMFRFGKHRKDDKMEKMGRIKIQDSFTSEEDRVRMKEEQERIQAKTREFRERQARERDYAEIQDFHRTFGCDDELLYGGMSSYDGCLALNARPQSPREGHLMDTLYAQVKKPRSSKPGDSNRSTPSNHDRIQRLRQEFQQAKQDEDVEDRRRTYSFEQSWSSSRPASQSGRHSVSVEVQVQRQRQEERESFQQAQRQYSSLPRQSRKNASSVSQDSWEQNYAPGEGFQSAKENPRYSSYQGSRNGYLGGHGFNARVMLETQELLRQEQRRKEQQLKKQPPADGVRGPFRQDVPPSPSQVARLNRLQTPEKGRPFYS