Protein: E3 ubiquitin-protein ligase rnf213-alpha (EC 2.3.2.27) (EC 3.6.4.-) (E3 ubiquitin-lipopolysaccharide ligase rnf213-alpha) (EC 2.3.2.-) (Mysterin-A) (Mysterin-alpha) (RING finger protein 213-A) (RING finger protein 213-alpha) (A0A0R4IBK5)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity (By similarity). Acts as a key immune sensor by catalyzing ubiquitination of the lipid A moiety of bacterial lipopolysaccharide (LPS) via its RZ-type zinc-finger: restricts the proliferation of cytosolic bacteria, such as Salmonella, by generating the bacterial ubiquitin coat through the ubiquitination of LPS (By similarity). Ubiquitination of LPS triggers cell-autonomous immunity, such as antibacterial autophagy, leading to degradation of the microbial invader (By similarity). Involved in lipid metabolism by regulating fat storage and lipid droplet formation; act by inhibiting the lipolytic process. Also regulates lipotoxicity by inhibiting desaturation of fatty acids (By similarity). Also acts as an E3 ubiquitin-protein ligase via its RING-type zinc finger (By similarity). Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of proteins downstream of rspo3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression (By similarity). Also has ATPase activity; ATPase activity is required for ubiquitination of LPS (By similarity). Also involved in neuromuscular regulation.
Subcellular location: Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q63HN8}. Lipid droplet {ECO:0000250|UniProtKB:Q63HN8}.
Interaction partners:
Sequence:
MKCPKCSHEALEKAPKFCSECGHKLQSQSYETTQGTPHDKSQTPSIVPQITNAEMDETGSESKSLEIQNANVSPKRPNENTSPNPKKKKRKKRKKEKKKKSGVSEGPSSLTSDLSDISLTDKEKKMDTDQSSDSDCSSCIVEDTPTPAEPSSHLSPPENETAGPAQLSASALTTGSSKDGEESIGTTQKPVSASASKAPLGVDQQTKEEKVKCKDEGQKSLSAKAQHTPNANVDQNANVQSDANIDKDSQNVEPQKSSSVKTKPSKSTVADPKKTESEKQKSGERDNENSTQPVSSPKLKRNQTEESQKMVFGPNSAPKKNRGSSADSAMKVEKKPAGGKKDSSADQKSKESEDTESQCVTLPKRNTRSTQHISSSDRLTIYFHAVLSKDFKFNPEEDLIFIRAGGPIGNWEENLVELSVSRDLKEHGFLVEGKFICRKIDAEAVSIPYKYVVYKQKKNKYDYEYIYKLDAEVPTNRCLFIKSHLLNDEGEWHQYDDIICAQPAKNMFEWVKKTIWSDEKKNVLQGRKIAGTIMLETIFDLLRSWSKINLNNFFSQLRQFYEIYRNPFVFEKKQTKWYQLDYDEKDVRELLKNFMLMHVTPELQKDSNEKSKFIQEPLKAGLIMLYVWKQYDLKLDYGTLSRLCTAVCLPNLPKDEFLSLWTDITESFSVINSFSDMVEALISKLKAENMPRWIIVIPLLHLLKGTSKPFEQVITKVNSKYEQSWAGLQGLRSNILSPGPQERRAMLNLMKTYGHLVEVDRLLIRSWMYLMPLDDLVECGSIFPVELLDILQLFTMKCPNNISFTSSESTAEALAHIQSQLLQHRYSCPDVDYGIQCIQAACKLLEKICSLVRYFGHSQNFTDIPVACMNLVASVSGFAQTKQEADTFAEKTLVLLNDTKQTVRAWMRQTFKGRLLNSHLFSSHLTGTSFSTETEVWNNIIAIDFVCKDFIKEWRDTFTTDFEGKYQQEDHLDQIEAYCSNIEKLKVSQPYLVNSVEKCALQAVSTICQTKSEWKLFARFNKFRINWRFGNLVSTIILKSWPKDDKGTYFEEEEAVLKHLLGWAAAKNIFQLHGADEKLIDQLSDEAKEKFAMATSLFTNVLNQLVTGKIKMKLLNHILEKKSVFLELLTLDCFSEEEQYKDIDAMKALIQTRQEEVKAIYHERALAGALIAMCHNVEEHVKVDYKYLEDLYSNDMNEMDLDLFMDVHELNQIPTEASLEVPYFELQDDVRSMAEILNIFKDSYIFKLRWGNEAALFVERAEDEELDELDELPITLDVLNEEIFLPCHAAYRNIYTSLKDGSIDFEDIDEIFRAYKGKYEKLAAEVAIMSKQDFNDDQHWVQTRIQQIKQYHELHLAVESAKVVMMVKETLCLQGDFQVLEKLLITTHSDFKSERLDSIDNELIQAKNVLVDITEPRRLCLQELGHRKNFVIWVKEALEDINELKVFVDLASISAGENDLDVDRVACFHDAVLGYSSMLYDLKPDAGFSLFNEMLKKLWKALDNDSNLPKKLCDSARHIEWLKTVKDSHGSVELSSLSLASAINSKGIYVINAQNQKKLALENILKLHIMEEHDGGCETRVYSLEDLRDLQNKLMLMSGKGEQGQCEVDQFAEVFASVQRLVSAFIDLYVAGNPLFRHWEANINCNSKEACIIIDFNLGSVVSVVMVEGDVTEQLPEVCKKMESCLRFWQDFMDKQRSQHYYLNYYTAEQLVYLCHQLAHNNMEEIDDQVLMMLSFIKPSCSTSDLRKAWHILQYDLIRKGPDQNDDLDFQTFVEVSSMTENESTEKSCPTSDDLIQQLGDASGSTKLGVIWNNYMRDMKAFLPDSLDVPSLGYLLEILANSHREDEGDMSQRDKTRTILRELPNGIASGRPNLIICPSEEILISCISIYMNSKNEPLPTYDEVLLCSATTPYEEVELFLRRCLSAGYRGKKIYTMLYVNQLNYEVSYKVEKFFQNQNAHTTNDYRLVLICESNKEHAYLPSAFSQFRLHLIPQQPIPSIQQYLHRHFAVPVGISSAAAVFKDRQNVGVVSSERSGVGKSLYIKRLYEKLKLNSKKPSQLKCIRLTEPKVDENVIIQSLISVLKKNDLSVYHFDVTTMVKKGLHEFLFRLLILGYLMDSKGNMWKSSNKHLYVIEILRPGLSQNDRRAGAKVSFNFLDVFPIVYCRSPKEVLELEMRMEEHPSFGLSDDPLMDDQEFRSEAYQRPYQYLQRFYNGINLDEFLYQGVEGSHVECLQMLFEYCGIIDPSWAELRNFAWFLNLQLQDCEKSVFCDFSFVGDTLLGFKNFVVEFMILMAKDFATPSLSISDQSPGRLHEDFSSANEEDLAPFKIRKRWESEPHPYIFFNDDHDSMTFIGFHLQPNAQKGVDAVDPSNNRVIKQNIMTMELYEGLKLQRVPFNIDFDQLPRWEKIERLSRVLGIQWPLDPDETYELTTDNMLKMLAVHMRFRCGIPVIIMGETGCGKTRLIKFLCEMHRSGVATDNMKLVKVHGGTSSEMIYTKVREAEAMALRNKLDYGFDTVLFFDEANTTEAISSIKEILCDNSAEGQNLTENTGLKIIAACNPYRKHTDVMIKRLESAGLGYRVRAEETDEKLGSIPLRQLVYRVQALPPSMIPLIWDFGQLNDHTEKMYIKQIVERVAETHSIDSGYITVITDVLSASQKYMRTRQDECSFVSLRDVERCMQVFGWFYKKHLMLLSELDKFESIQRTEKTDQHPKDTDERNPILWSLLMAVGVCYHACLEDKEKYRKKICKYFPAAYSPMKVMQEISVIQDIFLEGVPMGENIARNNALKENVFMMVICIELRIPLFLVGKPGSSKSLSKTLVADGMQGQAAHSDLFRKLKQIHLVSFQCSPHSTPEGIINTFKQCARFQEGKNLSEYVSVVVLDEIGLAEDSQKMPLKTLHPLLEEGCIDDQPSPHKKVGFIGISNWALDPAKMNRGIFVSRGDPDENELIESAKGICSSDVMILEKVRECFKPFAHAYLRICKKQEKGFFGLRDYYSLIKMMFAVAKACDQKPSAEQIVKAVLRNFSGKDDVDAVTFFTSRLNIKPELETISAIELVRENVTAIGQDEECRYLLVLTKNYAALRILQQTFFSDQCQPEIIFGSSFPKDQEYTQICRNINRVKICMETGQTIVLLNLQNLYESLYDALNQYYVTLGGQKYVDLGLGTHRVKCRVHKDFRLIVIEEKDIVYKQFPIPLINRLEKHYLDLNTLLKSEQKDIVKNLEQWVQCFTDVKNKHSVAPSARRYSPADAFIGYHTDTCASVVMQVTEQLKGQELSDPRKGILDESKLILLNCATPDAVVRLDCTSLFNVESEHLSRVYFEDQMHNSLAEFILSHIQQEGCSGAFFTEVTTFSRLLTASETQQLQNVVQNIELLSLQQFDTEQSFLKKIKNYLENTTGDKILLIQTDFDEGFQKLNVIASAKYSSINEINKFKKEGSGKIFVYFITKLPRMDGGTSYIGFNGGPWKSIHIDDLRRPKDIVSDIKALQGLTISQLFEEKAEKVDETEAMEVEDMYAGGEDEEDEEKMELEENNGCKDVLDTTALVRSCVQSAVGMLRDQTEGGMRSTKRVEILLMLLAEDQTLQAEFLKTLKTRLHSLLVAHDDNTISAKSWVSREALNVDALHEGGTFRHALWRRVQAVVTPFLAQLVSVVDRDCNLDLLLDRNSGEPLKKLWLEIFRDDKFLSVSPYTRTENNSATKTILVQNYMSVDRNKGCTMPFSWRIKDYLEDLWKHALQQEGHTVKQFEEFFWKTPLGRYISEATNEMQMEFFYRYLQDFISMTMNVTSEVDFEVLRGAFTSSVNEVRIAHEAHESEALSLVWIHVAYHHFKNRIQNLHRMMSLEPQISQMLLENRYASEGKELVLDVLAAVACIEYLEPQNLDGDDQSLAWLRRVKKLQVPVELVCSLESLHNRGDRCRQMVTNIQHGWRRIYSLVLFVEHMLLGVGDLQQKLKPVVLEHTQLLAQVLEQDSNLKKKKPFEAVITVLKTCKDKASQRIIRFGLQLCPVCMGDPRDPLSLPCDHIYCLTCIRQWLVPGQMHCPLCVQEVPDNFELKPSDELRRLISQNASFRMRCNAFFIDLVSTMCFKDNTPPSKDIILHLLSLLMVEASSLPPFKGRDRRFLTKALSPFDDSVDKNPVVRSVVLKLLLNYSFDHVKDYLQQHLTEVEQSKILEETDKAELYCLYMNCLEDSMYDRTQWHTVAEQQNCFLEETRFLLEFLQSDSVSAHTATVEHLQRLARVRLCLDMAADLLVANAGIHDDPSAFIQAFWNNVVNLCRQSRNDWYRVYLIRKLCSLQGVECVKNLLLQETYRWLFPQEILEMNQDDSQIDQYLACGADYKTIRDAVAKFMLDLHINGIQKAIEDCNCTPMKKAVYVLMAFFREVTSLHRTGNPNMHPKPEHCAGLEHFIKNSAIFVNNEMKAFAEKLVRNQLGALRVRPHMPSRDLSLVEVTIHMAAVLLCGNLLLLQPLQKLALSPNNMMASFIPTMPDDMLAVAQQAMGHLQWYFCPNGHPCTVGECGQPMEVSRCPDCDAEIGGSNHRPVDGFRAMQIQADRTQSGHILGDAQRRDLPDMQDTKNMSPAPFALLRLLTHMSMLIGTQNNPQSIMQIIKPAVVHPDAFLMQHLLKDMEQLSKALGKGVDDTVSTIHLAIHSLLEPHQTSQWPDPYDPNLSTKDARNGWENAMNNDVITHHLKVLEHQLKEVNAFIREDERVSSNPVMKLTFGEPGRFLRSLPQNSLIHNSSIWSCRNKVSLMSLTHIVEQNNGRDTLPVLWRFLQREAELRLVRFLPDILVLQRDLVKKFQNITDLTYKTIREFLQDQKAASLTAWYEKRIKIFLTTWNQIRVSLANTGEIKLPADYTEKDLGLDADLQVLLPQRRGLGLCSTALVSYLITIHNDLMYTVEKHTGDDSDYKISPAELTELHVIRYEYDRDLLPLVLANCQYSMECGQETLLEYDLPKIQQQILTRFLQGKPLITINGIPTLVNRQDRNYEIIFKDVKGKVQQELLQPLTQYDLVKELQSYSDVCEALSTVELAVGFLAMTGGEPNMQLGVYLKDVLQMTDHMATHVFKALSRCSLKHCVALWQLLSSLKSETMLRLKRDPFVGISKEYKQPLQEEHKRLLTSFFTKSSADAFLLEMHEFLLLVLKSPKATDTYRPDWRLKHTVVSYMERKDLDVPPEVEEFFPKEILLSEYTSTWNFSVNLRQKRSQS