Protein: Exosome complex component 10 (EC 3.1.13.-) (Autoantigen PM/Scl 2 homolog) (Polymyositis/scleroderma autoantigen 2 homolog) (P56960)
Organism: Mus musculus (Mouse)
Function: Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of MTREX, C1D and MPHOSPH6 with the RNA exosome involved in the maturation of 5.8S rRNA (By similarity).
Subcellular location: Cytoplasm {ECO:0000269|PubMed:29118343, ECO:0000269|PubMed:34965385}. Nucleus {ECO:0000269|PubMed:29118343, ECO:0000269|PubMed:32313933, ECO:0000269|PubMed:34965385, ECO:0000269|PubMed:36923944}. Nucleus, nucleolus {ECO:0000269|PubMed:29118343, ECO:0000269|PubMed:34965385}. Nucleus, nucleoplasm {ECO:0000269|PubMed:34965385}. Note=Strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form (By similarity). In oocytes, the protein is diffusely distributed in the cytoplasm, in zygotes it is found in both the cytoplasm and pronuclei and from the two-cell stage onward the protein accumulates in the nucleus, especially at the nucleolus precursor body periphery (PubMed:34965385). In metaphase blastomeres that lack structured nuclei, the protein localizes diffusely in the cytoplasm (PubMed:34965385). In spermatocytes, the protein accumulates in the nucleolus during zygotene, late pachytene and diplotene sub-stages and in the cytoplasm during metaphase I (PubMed:29118343). {ECO:0000250|UniProtKB:Q01780, ECO:0000269|PubMed:29118343, ECO:0000269|PubMed:34965385}.
Interaction partners:
- Exosome complex component RRP43
- Exosome complex component RRP46
- Exosome complex component RRP45
- Exosome complex component RRP41
- Exosome complex exonuclease RRP42
- Exosome complex component RRP4
- Exosome complex component CSL4
- Exosome complex component RRP40
- Exosome complex exonuclease RRP44
- Nuclear nucleic acid-binding protein C1D
Sequence:
MAPPSPREHQSAPATSATKPDAEMVLPGFPDADSFVKFALGSVVAVTKASGGLPQFGDEYDFYRSFPAFQAFCETQGDRLLQCMSRVMQYHGCRSNIKDRSKVTELEDKFDLLVDTNDVILERVGMLLDEASGVNKHQQPVLPAGLQVPKTIVSSWNRKAGEYGKKAKSETFRLLHAKNIVRPQLRFREKIDNSNTPFLPKIFVKPNARKPLPLALSKERRERPQDRPEDLDVPPALADFIHQQRTQQVEQDMFAHPYQYELDHFTPPQSVLQRPKPQLYRAVGETPCHLVSSLDELVELNEKLLGCQEFAVDLEHHSYRSFLGLTCLMQISTRTEDFIVDTLELRSDMYILNESLTDPAIVKVFHGADSDIEWLQKDFGLYVVNMFDTHQAARLLNLARHSLDHLLRLYCGVESNKQYQLADWRIRPLPEEMLSYARDDTHYLLYIYDRMRLELWERGNHQPVQLQVVWQRSRDICLKKFVKPIFTDESYLELYRKQKKHLNSQQLTAFQLLFAWRDKTARREDESYGYVLPNHMMLKIAEELPKEPQGIIACCNPVPPLVRQQINEMHLLIQQAREMPLLKSENAAGVRKSGPLPSAERLENDLFGPHDCSHAPPDNYQNTSTDGTLPLQKQPSLFTEGKEETSVDAGCLLATAVITLFSEPNTEEGGKTPLTVAQKKAQNIMQSFENPFRMFLPSLEHKAHISQAAKFDPSSKIYEISNRWKLASQVQVQKEPKEATKKKVAEQTAAREEAKEEAAAGVLEQAIPVRQQAALENATKKRERATSDLRTIEQKQEKKRLKSSKKAKDPDPPGKDFSPYDYSQSDFRAFAGDSKSKPSSQFDPNKLAPSGKKGVGAKKCKQSVGNKSMSFAVGKSDRGFRHNWPKR