Protein: Ras guanine nucleotide exchange factor glfB (GEF-Like protein B) (Q54L90)
Organism: Dictyostelium discoideum (Social amoeba)
Function: GpaB-activated, rapA-specific guanine nucleotide exchange factor, involved in the regulation of the balance between Ras and Rap signaling at the leading edge of chemotaxing cells. Spatially localized activation of Rap and Ras induces F-actin polymerization at the leading edge of chemotaxing cells through the Rac, PI3K, and TORC2 pathways. Acts also as a key regulator of actin-driven membrane protrusions during processes such as phagocytosis and cytokinesis, possibly by modulating rapA signaling pathways.
Subcellular location: Cytoplasm, cell cortex {ECO:0000269|PubMed:27237792, ECO:0000269|PubMed:28778987}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:28778987}. Cell projection, filopodium {ECO:0000269|PubMed:28778987}. Cell projection, lamellipodium {ECO:0000269|PubMed:28778987}. Note=During chemotaxis, localizes at the leading edge of cells, whereas in response to cAMP stimulation, rapidly and transiently translocates to the cortex (PubMed:27237792). Localizes to F-actin-rich regions (PubMed:28778987). During macropinocytosis, strongly localizes to crowns and remains there for a short time after the cups were enclosed (PubMed:28778987). During phagocytosis of heat-killed yeast, strongly localizes to phagocytic cups with F-actin (PubMed:28778987). During cytokinesis, mainly colocalizes with F-actin at filopodia or lamellipodia of daughter cells (PubMed:28778987). {ECO:0000269|PubMed:27237792, ECO:0000269|PubMed:28778987}.
Interaction partners:
Sequence:
MTDLNSESFSALNFWKSVEKKNISTNFQGTKVVAPSKKINSFPLLAPPAPPPPPTEQEINIGSGNSTFISSNNNNSNNNNNNNSNNNNNNNLNNSNNNNNNLNSNNNNNNNNNNNNNNGNNNNNSNFLTRQDSSTQKEWDEQNVTEAFGFWKQKAVQLQKETERYNARRNARQTIDLTNILRKSTSSDLLIKPPVESPPLTPVGQDDEGEQQQQQQQQKQSSPSTPSNDTDTETTAAAVTTTTTTTTTTTTSTTTTTTETVLQANQLEIKYGGETIAVVDDSGTTPRDYRRSRSISCEIIPKINGVITTSPQRVTTTTTTTTPSTGGVVVADEESDSSEEESDSSEEESDEYTDEESETELQVVSNATPRRSDDFTPTIVESPPLTSVNSNDNTSSGTVVAPIDLNSSTGGNTGSQQPPQPSSQQQKPDQASENTAVAASSISATTNVTSAASTTTVAPDSIINTKDVTVVSSTLTTTTSATSSTTSATTQSIPAPPSPSQQRAAQSISTSSVTPAAITKPTKDAKDKKDPAKKSIGATLTRTVTKTFIRDSKENNKVPTGTSPPVSSSTSISSSTGIKKDKVKLSKEEKDRIKKEKSAKKKKEKDEKKQQKTNKKALTKNNSSTDVKKGFVSPQQQQILDSPYRIYGVRLTQLVLSNDGDLPAILTQTITVLSNSNKLDVNSVFVGAENEPAVREIRKRSDLERIDFSIIGDPRVVAGLLILFFAELPQPLFNSKFFGDLVEINDITNPQVKLNDLKQLINSLSQLRRSLLQILVTFFTSKYINGNSVTTRAIAIQSIAQSFGPQFFRGTSSSDSDIQVGIETLKLIIDNYVFLFEKTNEPDVKYKNIDGKMIISEGSIDKLIDKATDQYYPYNEKYFSLTFFITHLFFIQPHELADKLITLYRENLDTLETKKKWKKHRRSKKASFINEAVKLWVDYCYKEMREDKELSKKILKGFPHLEAQLASRLSHRTTINDFLKLPKRVHSRTRSASFSDTLLSTGGIGSTSGGIGGGVNNCLLSAMEIAEQCTLVDYDLFTNVRLSDWVRLVQGSVDPQTAPSLSLALKRSTIWAQWAMGEILSTEDKSQRVAIINLLVDVAINCKDLANFNTAISIHTALTNHHIKRLQQTWDSVPKETLNKITQLEQSLQVWLKPDATNPFGVICQSINSACVPNFSILRTILSQIDQKIPTFSNDGSMVNVEKLRTIFGIVVEIQRLQQQRNYTMKPTKLFIQLQDINTVSMDELADLSLKCEPPVSKAKKYNAPADIVDEDWRLKITKTFNKPLATTSVGIDLPRLASSFTFNTTGHKTTPEEKSAYGNKIQDIFHVLVSLAQIESSELETDVREKFTTYIPMSTDPDSDFKRELLKFLDEVCHADNSKLVRVLKCCNQAIIAPVIIEITLNIAKGVPFMDAGGWRILISNINNSNNSVILDKIDEINEDSSNVEKEKLSSSQEQQEQQEQKQQEQQQQQQEPQPLFIRHYKKQRSRSAQSKDFFEFEWFIQLNLDADCKNILSFDLKISNLVFSTDTSPNIRDQLLESFKSYLVSQECVQYINFNENKQPVAAPVTPATTIVTTKEESTTVTSSTTTVVQESVPSTNAE