Protein: Enhancer of filamentation 1 (mEF1) (CRK-associated substrate-related protein) (CAS-L) (Neural precursor cell expressed developmentally down-regulated protein 9) (NEDD-9) (p105) (O35177)
Organism: Mus musculus (Mouse)
Function: Scaffolding protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion (By similarity). As a focal adhesion protein, plays a role in embryonic fibroblast migration. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRKl and SHPTP2 to the tyrosine phosphorylated form (By similarity). Promotes adhesion and migration of lymphocytes; as a result required for the correct migration of lymphocytes to the spleen and other secondary lymphoid organs. Plays a role in the organization of T-cell F-actin cortical cytoskeleton and the centralization of T-cell receptor microclusters at the immunological synapse. Negatively regulates cilia outgrowth in polarized cysts (By similarity). Modulates cilia disassembly via activation of AURKA-mediated phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin (By similarity). Positively regulates RANKL-induced osteoclastogenesis. Required for the maintenance of hippocampal dendritic spines in the dentate gyrus and CA1 regions, thereby involved in spatial learning and memory.
Subcellular location: Cytoplasm, cell cortex {ECO:0000250|UniProtKB:Q14511}. Nucleus {ECO:0000250|UniProtKB:Q14511}. Golgi apparatus {ECO:0000250|UniProtKB:Q14511}. Cell projection, lamellipodium {ECO:0000250|UniProtKB:Q14511}. Cytoplasm {ECO:0000250|UniProtKB:Q14511}. Cell junction, focal adhesion {ECO:0000269|PubMed:25059660}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q14511}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000250|UniProtKB:A0A8I3PDQ1}. Cell projection, cilium {ECO:0000250|UniProtKB:Q14511}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q14511}. Basolateral cell membrane {ECO:0000250|UniProtKB:A0A8I3PDQ1}.
Interaction partners:
- [F-actin]-monooxygenase MICAL1
- Aurora kinase A
- Dedicator of cytokinesis protein 3
- Proto-oncogene tyrosine-protein kinase Src
- Mothers against decapentaplegic homolog 3
- Breast cancer anti-estrogen resistance protein 3 homolog
- [F-actin]-monooxygenase MICAL3
- Adapter molecule crk
- Focal adhesion kinase 1
- [F-actin]-monooxygenase MICAL2
Sequence:
MWARNLMARALYDNVPECAEELAFRKGDILTVIEQNTGGLEGWWLCSLHGRQGIVPGNRVKLLIGPVQETPGHEQPTPGPMHQTFGQQKLYQVPNSQAASRDTIYQVPPSYQNQGIYQVPTGHGTPEQDVYQVPPSVQRNIGGTNGPLLSKKVITPVRTGHGYVYEYPSRYQKDVYDVPPSHSTQGVYDIPPSSVKGPVFSVPVGEIKPQGVYDIPPTQGVYAIPPSACRDEAGLREKEYDFPPPMKQDGKPDTRPEGVYDIPPTSTKTAGKDLHIKFPCDAPGGVEPMARRHQSFSLHHAPSQLGQSGDTQSDAYDVPRGVQFLEVPTETSEKANPEERDGVYDVPLHNPADAKGSRDVVDGINRLSFSSTGSTRSNMSTSSTSSKESSLSASPSQDKRLRLDPDTAIEKLYRLQQTLEMGVCSLMSLVTTDWRCYGYMERHINEIRTAVDKVELFLREYLHFAKGALANASCLPELVLHNKMKRELQRVEDSHQILSQTSHDLNECSWSLNILAINKPQNKCDDLDRFVMVAKTVPDDAKQLTTTISTYAETLFRADPANSHLKNGPNSIMNSSEYTHPGSQMQPLHPGDYKAQVHSKPLPPSLSKDQPPDCGSSDGSERSWMDDYDYVHLQGKEEFERQQKELLEKENIMKQSKAQLEHHQLSQFQLLEQEITKPVENDISKWKPSQSLPTTNNSVGAQDRQLLCFYYDQCETHFISLLNAIDALFSCVSSAQPPRIFVAHSKFVILSAHKLVFIGDTLTRQVAAQDIRNKVRNSSNQLCEQLKTIVMATKMAALHYPSTTALQEMVHQVTDLSRNAQLFKRSLLEMATF