Protein: cGMP-dependent protein kinase egl-4 (EC 2.7.11.12) (Egg-laying defective protein 4) (O76360)
Organism: Caenorhabditis elegans
Function: Promotes chemoreceptor gene expression in response to increased cGMP levels by antagonizing the gene repression functions of the class II HDAC hda-4 and the mef-2 transcription factor. Regulates gene expression via recruitment of a histone deacetylase complex containing hda-2, saeg-1 and saeg-2. Represses body size and lifespan through the dbl-1 and insulin pathways, respectively. May also signal through daf-3 and/or daf-5. Role in egg-laying, dauer formation and motility. Regulates behavioral responses to various chemosensory stimuli in sensory neurons. Required for the initiation of long term adaptation to prolonged odor exposure which results in a decrease in odor seeking behavior. May regulate this process by phosphorylating tax-2, a subunit of cyclic nucleotide-gated channel tax-2/tax-4. In ASH sensory neurons, negatively regulates avoidance behavior to some bitter tastants, such as quinine, probably by phosphorylating rgs-2 and rgs-3 which are 2 regulator of G-protein signaling proteins. In AWB sensory neurons, involved in avoidance behavior to some repellent odors. In ASE left (ASEL) sensory neuron, involved in the sensing of environmental alkalinity downstream of receptor-type guanylate cyclase gcy-14. In sensory neurons, involved in the signaling pathway downstream of insulin, TGF-beta and receptor-type guanylate cyclase responsible for inducing quiescence after food intake. Might play a role in aversive olfactory learning in AWC neurons when an odor is associated with food deprivation, depending on the ins-1/age-1 signal from the AIA to the AWC neurons. Probably by regulating neuronal transmission downstream of lin-3 and receptor lin-23 and phospholipase plc-3 in ALA neurons, involved in the decrease in locomotion during the quiescent state that precedes each larval molt.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:20220099, ECO:0000269|PubMed:22319638, ECO:0000269|PubMed:23874221, ECO:0000269|PubMed:23954825, ECO:0000269|PubMed:27383131}. Nucleus {ECO:0000269|PubMed:20220099, ECO:0000269|PubMed:21573134, ECO:0000269|PubMed:22319638, ECO:0000269|PubMed:23874221, ECO:0000269|PubMed:23954825, ECO:0000269|PubMed:27383131}. Note=In resting AWC sensory neurons, localizes in cytoplasm (PubMed:20220099, PubMed:23954825, PubMed:27383131). Prolonged exposure to attractive odorants sensed by AWC neurons results in nuclear translocation (PubMed:20220099, PubMed:23954825, PubMed:27383131). Nuclear translocation is required for the adaptation to prolonged odor exposure and is controlled by G(o)-alpha subunit protein goa-1 (PubMed:20220099, PubMed:23954825, PubMed:27383131). Localization is regulated by cGMP levels: high cGMP levels result in cytoplasmic localization whereas low cGMP levels result in nuclear localization (PubMed:20220099, PubMed:22319638). Nuclear localization in AWC neurons is dependent on age-1 (PubMed:27383131). In addition, an intact sensory cilia structure is required for cytoplasmic localization in resting AWC neurons (PubMed:22319638). In resting AWB sensory neurons, constitutive nuclear localization is dependent on goa-1 (PubMed:23954825). In resting ASH sensory neurons, localizes in both cytoplasm and nucleus (PubMed:23874221). Cytoplasmic localization is important for negative regulation of quinine sensitivity in ASH neurons (PubMed:23874221). {ECO:0000269|PubMed:20220099, ECO:0000269|PubMed:22319638, ECO:0000269|PubMed:23874221, ECO:0000269|PubMed:23954825, ECO:0000269|PubMed:27383131}.
Interaction partners:
- Receptor-type guanylate cyclase gcy-10
- Cyclic nucleotide-gated channel
- cGMP-dependent protein kinase
- Receptor-type guanylate cyclase daf-11
- Guanine nucleotide-binding protein alpha-17 subunit
- Rap-GAP domain-containing protein
- Putative 6-pyruvoyl tetrahydrobiopterin synthase
- Cyclic nucleotide-binding domain-containing protein
- glutamate synthase
- Serine/threonine-protein phosphatase 2B catalytic subunit
Sequence:
MSSGSRPSSGGGGGGGGASGGAGGGAPGGGGGGIRGFFSKLRKPSDQPNGNQVQVGTRTFEAHELQKLIPQLEEAISRKDAQLRQQQTIVEGHIKRISELEGEVTTLQRECDKLRSVLEQKAQSAASPGGQPPSPSPRTDQLGNDLQQKAVLPADGVQRAKKIAVSAEPTNFENKPATLQHYNKTVGAKQMIRDAVQKNDFLKQLAKEQIIELVNCMYEMRARAGQWVIQEGEPGDRLFVVAEGELQVSREGALLGKMRAGTVMGELAILYNCTRTASVQALTDVQLWVLDRSVFQMITQRLGMERHSQLMNFLTKVSIFQNLSEDRISKMADVMDQDYYDGGHYIIRQGEKGDAFFVINSGQVKVTQQIEGETEPREIRVLNQGDFFGERALLGEEVRTANIIAQAPGVEVLTLDRESFGKLIGDLESLKKDYGDKERLAQVVREPPSPVKIVDDFREEFAQVTLKNVKRLATLGVGGFGRVELVCVNGDKAKTFALKALKKKHIVDTRQQEHIFAERNIMMETSTDWIVKLYKTFRDQKFVYMLLEVCLGGELWTTLRDRGHFDDYTARFYVACVLEGLEYLHRKNIVYRDLKPENCLLANTGYLKLVDFGFAKKLASGRKTWTFCGTPEYVSPEIILNKGHDQAADYWALGIYICELMLGRPPFQASDPMKTYTLILKGVDALEIPNRRIGKTATALVKKLCRDNPGERLGSGSGGVNDIRKHRWFMGFDWEGLRSRTLKPPILPKVSNPADVTNFDNYPPDNDVPPDEFSGWDEGF