Protein: Guanylate-binding protein 1 (EC 3.6.1.-) (EC 3.6.5.-) (GTP-binding protein 1) (GBP-1) (HuGBP-1) (hGBP1) (Guanine nucleotide-binding protein 1) (Interferon-induced guanylate-binding protein 1) (P32455)
Organism: Homo sapiens (Human)
Function: Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions (By similarity). Confers protection to several pathogens, including the bacterial pathogens Listeria monocytogenes and Mycobacterium bovis BCG as well as the protozoan pathogen Toxoplasma gondii (By similarity). Promotes IFN-gamma-mediated host defense against bacterial infections by regulating bacteriolytic peptide generation via its interaction with ubiquitin-binding protein SQSTM1, which delivers monoubiquitylated proteins to autolysosomes for the generation of bacteriolytic peptides (By similarity). Exhibits antiviral activity against influenza virus.
Subcellular location: Cytoplasmic vesicle membrane {ECO:0000269|PubMed:29144452, ECO:0000269|PubMed:29233899, ECO:0000269|PubMed:31268602}; Lipid-anchor {ECO:0000269|PubMed:21151871}; Cytoplasmic side {ECO:0000269|PubMed:21151871}. Golgi apparatus membrane {ECO:0000269|PubMed:15937107}; Lipid-anchor {ECO:0000269|PubMed:21151871}; Cytoplasmic side {ECO:0000269|PubMed:21151871}. Cell membrane {ECO:0000269|PubMed:21151871}; Lipid-anchor {ECO:0000269|PubMed:21151871}; Cytoplasmic side {ECO:0000269|PubMed:21151871}. Cytoplasm, cytosol {ECO:0000269|PubMed:21151871, ECO:0000269|PubMed:37797010}. Secreted {ECO:0000269|PubMed:16936281}. Note=Localizes to pathogen-containing vacuoles or to the cell surface of bacteria that escaped vacuoles (PubMed:29144452, PubMed:31268602, PubMed:32510692, PubMed:32581219). Secreted from endothelial cells in the cerebrospinal fluid, upon bacterial challenge and independently of IFNG induction (PubMed:16936281). Golgi membrane localization requires isoprenylation and the presence of another IFNG-induced factor (PubMed:15937107). Sequestered in the cytosol following phosphorylation by PIM1 and subsequent interaction with 14-3-3 protein sigma (SFN) (PubMed:37797010). {ECO:0000269|PubMed:15937107, ECO:0000269|PubMed:16936281, ECO:0000269|PubMed:29144452, ECO:0000269|PubMed:31268602, ECO:0000269|PubMed:32510692, ECO:0000269|PubMed:32581219, ECO:0000269|PubMed:37797010}.
Interaction partners:
- Signal transducer and activator of transcription 1-alpha/beta
- Guanylate-binding protein 2
- Guanylate-binding protein 3
- Guanylate-binding protein 5
- Guanylate-binding protein 4
- Interferon-induced protein with tetratricopeptide repeats 3
- C-X-C motif chemokine 10
- Interferon-induced helicase C domain-containing protein 1
- Interferon-induced protein 44
- Interferon-induced GTP-binding protein Mx1
Sequence:
MASEIHMTGPMCLIENTNGRLMANPEALKILSAITQPMVVVAIVGLYRTGKSYLMNKLAGKKKGFSLGSTVQSHTKGIWMWCVPHPKKPGHILVLLDTEGLGDVEKGDNQNDSWIFALAVLLSSTFVYNSIGTINQQAMDQLYYVTELTHRIRSKSSPDENENEVEDSADFVSFFPDFVWTLRDFSLDLEADGQPLTPDEYLTYSLKLKKGTSQKDETFNLPRLCIRKFFPKKKCFVFDRPVHRRKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIQVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVQKAIAHYEQQMGQKVQLPTETLQELLDLHRDSEREAIEVFIRSSFKDVDHLFQKELAAQLEKKRDDFCKQNQEASSDRCSALLQVIFSPLEEEVKAGIYSKPGGYRLFVQKLQDLKKKYYEEPRKGIQAEEILQTYLKSKESMTDAILQTDQTLTEKEKEIEVERVKAESAQASAKMLQEMQRKNEQMMEQKERSYQEHLKQLTEKMENDRVQLLKEQERTLALKLQEQEQLLKEGFQKESRIMKNEIQDLQTKMRRRKACTIS