Protein: Histone-lysine N-methyltransferase set-2 (EC 2.1.1.354) (SET domain-containing protein 2) (Q18221)
Organism: Caenorhabditis elegans
Function: Histone methyltransferase that specifically di- and trimethylates 'Lys-4' of histone H3 at all developmental stages and in adult germ cells. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Implicated in the epigenetic inheritance of lifespan over several generations. Acts in the germline to limit the longevity of the soma, probably by regulating a lipid metabolism pathway that signals from the germline to the intestine, thereby preventing accumulation of mono-unsaturated fatty acids. Methylation in the germline is required for germline development and fertility, possibly by ensuring genome stability. May act redundantly with mes-3 and mes-4 proteins in the development of a fertile germline. Required for RNAi. Functions as an antagonist of hpl-1 and hpl-2 activity in growth and somatic gonad development. Cooperates with jmjd-3.1 and egl-27 to ensure robust transdifferentiation of the Y rectal cell to the PDA motor neuron during larval development.
Subcellular location: Nucleus {ECO:0000269|PubMed:11729150}. Note=Localized in mitotic and mid-late-stage meiotic nuclei but is undetectable in early pachytene nuclei.
Interaction partners:
- Set1/Ash2 histone methyltransferase complex subunit ash-2
- Retinoblastoma-binding protein homolog 5
- WD repeat-containing protein wdr-5.1
- Dosage compensation protein dpy-30
- CXXC-type zinc finger protein 1
- Anaphase-promoting complex subunit 4-like WD40 domain-containing protein
- JmjC domain-containing protein
- WD_REPEATS_REGION domain-containing protein
- Nucleosome-remodeling factor subunit NURF301-like
- Multifunctional procollagen lysine hydroxylase and glycosyltransferase
Sequence:
MSTHDMNHHPPRKSHSKRDKPSSSNSGPKIENHKCKWAWQKVFETGKSFLRRDGFPQDCKSKEDFERIKRTGVRKTSENMLEDPRKNFESLQQSSVYQTNSFRNPRYLCRAHLRVDSYYCTIPPKREVSLFNMDDNCTEVLLRDFAKDCGKVEKAYVCIHPETKRHMKMAYVKFATVKEAHNFYSMYHAQNLLATKCTPRIDPFLSILNEEYEVATNGQVLPILPDDLASIDPSVLRDLRANFLRDQNEKYELAMRNTYEDEGGMLSGVIMDTSDHYERDYTMDHDVGPSSMKMSPIPPPPIKEESPPPPPPPPVASVSNLAPVPSVQLPYYNNIQPSSSTMHMPEFRPTEPPPSYSREDPYRSTSRSSLSRHRNRSRSPSDGMDRSGRSSSRRTHRRPESRNGSKNANGDVVKYETYKMEKRKIKYEGGNKKYEQVHIKERTAVIRGKNQLENVSSESASGSSSVDTYPDFSDEERKKKKRPKSPNRSKKDSRAFGWDSTDESDEDTRRRRSGRSQNRSSERKFQTTSSSSTRRELSSTHTNSVPNLKSHETPPPPPPKGHPSVHLQTPYQHVQPQMIPATYYNLPPQHMAPPPITTSLPPFCDFSQPPPGFTPTFKPITNAPLPTPYQASNIPQPGLVQIAALSAAPEPFSSIPGPPPGPAPIQEDVGRAESPEKPSLSERFSGIFGPTQREEPAQVEVEYDYPLKHSESHDDRHSLEDMDVEVSSDGETVSNVEKIECMEEKKRQDLERIAIARTPIVKKCKKRMMDELSRKVAEDIRQQIMRQCFAALDEKLHLKAIADEEKRKKEREEKARQEAEKPSNHLIADMMTLYNNQSFASSSRGFYRKQKPIPKSHPKHQEHHHHAKASVSTPVHSSSTSRNSSVAPTPQRTVSTSSSSSSAATSARVSEDESDSDSTPGEVQRRKTSVLSNDKRRRRASFSSTSIQSSPERQRDVSSSSRTSSSSSTSSMKQEETADEKSRKRKLIMSSDESSTTGSTATSVVSSRQSSLEPQQEKTDGEPPKKKSQTDFISERVSKIEGEERPLPEPVETSGPIIGDSSYLPYKIVHWEKAGIIEMNLPANSIRAHEYHPFTTEHCYFGIDDPRQPKIQIFDHSPCKSEPGSEPLKITPAPWGPIDNVAETGPLIYMDVVTAPKTVQKKQKPRKQVFEKDPYEYYEPPPTKRPAPPPRFKKTFKPRSEEEKKKIIGDCEDLPDLEDQWYLRAALNEMQSEVKSADELPWKKMLTFKEMLRSEDPLLRLNPIRSKKGLPDAFYEDEELDGVIPVAAGCSRARPYEKMTMKQKRSLVRRPDNESHPTAIFSERDETAIRHQHLASKDMRLLQRRLLTSLGDANNDFFKINQLKFRKKMIKFARSRIHGWGLYAMESIAPDEMIVEYIGQTIRSLVAEEREKAYERRGIGSSYLFRIDLHHVIDATKRGNFARFINHSCQPNCYAKVLTIEGEKRIVIYSRTIIKKGEEITYDYKFPIEDDKIDCLCGAKTCRGYLN