Protein: Genome polyprotein [Cleaved into: Leader protein (L); Capsid protein VP0 (VP4-VP2); Capsid protein VP4 (P1A) (Virion protein 4); Capsid protein VP2 (P1B) (Virion protein 2); Capsid protein VP3 (P1C) (Virion protein 3); Capsid protein VP1 (P1D) (Virion protein 1); Protein 2A (P2A); Protein 2B (P2B); Protein 2C (P2C) (EC 3.6.4.13); Protein 3A (P3A); VPg (P3B) (Protein 3B); Protease 3C (P3C) (EC 3.4.22.28) (Picornain 3C); RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48) (3D polymerase) (3Dpol) (Protein 3D) (3D)] (P13899)
Organism: Theiler's murine encephalomyelitis virus (strain DA) (TMEV)
Function: Forms a complex with host RAN and probably binds to exportins carrying activated MAPK in order to mediate the hyperphosphorylation of host Phe/Gly containing nuclear pore proteins (Nups) resulting in cessation of active nucleocytoplasmic transport (Probable). Proteins with NLS signals fail to import, cellular mRNAs fail to export, and some proteins small enough for diffusion are not retained anymore (efflux) (By similarity). The resulting inhibition of cellular protein synthesis serves to ensure maximal viral gene expression and to evade host immune response (By similarity). The leader protein also inhibits host interferon regulatory factor 3 (IRF3) dimerization, thereby blocking the transcriptional activation of IFN genes. Binds to host RNase L thereby preventing its activation by 2'-5' oligoadenylates in order to counteract the antiviral interferon-inducible OAS/RNase L pathway.
Subcellular location: [Capsid protein VP2]: Virion {ECO:0000250|UniProtKB:C0MHL9}. Host cytoplasm {ECO:0000305}.; SUBCELLULAR LOCATION: [Capsid protein VP3]: Virion {ECO:0000250|UniProtKB:C0MHL9}. Host cytoplasm {ECO:0000305}.; SUBCELLULAR LOCATION: [Capsid protein VP1]: Virion {ECO:0000250|UniProtKB:C0MHL9}. Host cytoplasm {ECO:0000305}.; SUBCELLULAR LOCATION: [Protein 2A]: Host nucleus, host nucleolus {ECO:0000250|UniProtKB:Q66765}.; SUBCELLULAR LOCATION: [Protein 2B]: Host cytoplasmic vesicle membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are probably autophagosome-like vesicles. {ECO:0000305}.; SUBCELLULAR LOCATION: [Protein 2C]: Host cytoplasmic vesicle membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are probably autophagosome-like vesicles. {ECO:0000305}.; SUBCELLULAR LOCATION: [Protein 3A]: Host cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:P03304}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are probably autophagosome-like vesicles. {ECO:0000250|UniProtKB:P03304}.; SUBCELLULAR LOCATION: [VPg]: Virion {ECO:0000305}.; SUBCELLULAR LOCATION: [Protease 3C]: Host cytoplasm {ECO:0000305}.; SUBCELLULAR LOCATION: [RNA-directed RNA polymerase]: Host cytoplasmic vesicle membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are probably autophagosome-like vesicles. {ECO:0000305}.
Interaction partners:
Sequence:
MACKHGYPDVCPICTAVDVTPGFEYLLLADGEWFPTDLLCVDLDDDVFWPSNSSNQSETMEWTDLPLVRDIVMEPQGNASSSDKSNSQSSGNEGVIINNFYSNQYQNSIDLSASGGNAGDAPQNNGQLSNILGGAANAFATMAPLLLDQNTEEMENLSDRVASDKAGNSATNTQSTVGRLCGYGEAHHGEHPASCADTATDKVLAAERYYTIDLASWTTTQEAFSHIRIPLPHVLAGEDGGVFGATLRRHYLCKTGWRVQVQCNASQFHAGSLLVFMAPEFYTGKGTKTGDMEPTDPFTMDTTWRAPQGAPTGYRYDSRTGFFAMNHQNQWQWTVYPHQILNLRTNTTVDLEVPYVNIAPTSSWTQHANWTLVVAVFSPLQYASGSSSDVQITASIQPVNPVFNGLRHETVIAQSPIAVTVREHKGCFYSTNPDTTVPIYGKTISTPNDYMCGEFSDLLELCKLPTFLGNPNSNNKRYPYFSATNSVPTTSLVDYQVALSCSCMCNSMLAAVARNFNQYRGSLNFLFVFTGAAMVKGKFLIAYTPPGAGKPTTRDQAMQATYAIWDLGLNSSFVFTAPFISPTHYRQTSYTSATIASVDGWVTVWQLTPLTYPSGAPVNSDILTLVSAGDDFTLRMPISPTKWAPQGSDNAEKGKVSNDDASVDFVAEPVKLPENQTRVAFFYDRAVPIGMLRPGQNIESTFVYQENDLRLNCLLLTPLPSFCPDSTSGPVKTKAPVQWRWVRSGGTTNFPLMTKQDYAFLCFSPFTYYKCDLEVTVSALGTDTVASVLRWAPTGAPADVTDQLIGYTPSLGETRNPHMWLVGAGNTQISFVVPYNSPLSVLPAAWFNGWSDFGNTKDFGVAPNADFGRLWIQGNTSASVRIRYKKMKVFCPRPTLFFPWPVSTRSKINADNPVPILELENPAAFYRIDLFITFIDEFITFDYKVHGRPVLTFRIPGFGLTPAGRMLVCMGEKPAHGPFTSSRSLYHVIFTATCSSFSFSIYKGRYRSWKKPIHDELVDRGYTTFGEFFRAVRAYHADYYKQRLIHDVEMNPGPVQSVFQPQGAVLTKSLAPQAGIQNLLLRLLGIDGDCSEVSKAITVVTDLFAAWERAKTTLVSPEFWSKLILKTTKFIAASVLYLHNPDFTTTVCLSLMTGVDLLTNDSVFDWLKNKLSSFFRTPPPVCPNVLQPQGPLREANEGFTFAKNIEWAMKTIQSIVNWLTSWFKQEEDHPQSKLDKFLMEFPDHCRNIMDMRNGRKAYCECTASFKYFDELYNLAVTCKRIPLASLCEKFKNRHDHSVTRPEPVVVVLRGAAGQGKSVTSQIIAQSVSKMAFGRQSVYSMPPDSEYFDGYENQFSVIMDDLGQNPDGEDFTVFCQMVSSTNFLPNMAHLERKGTPFTSSFIVATTNLPKFRPVTVAHYPAVDRRITFDFTVTAGPHCTTSNGMLDIEKAFDEIPGSKPQLACFSADCPLLHKRGVMFTCNRTKAVYNLQQVVKMVNDTITRKTENVKKMNSLVAQSPPDWEHFENILTCLRQNNAALQDQLDELQEAFAQARERSDFLSDWLKVSAIIFAGIASLSAVIKLASKFKESIWPSPVRVELSEGEQAAYAGRARAQKQALQVLDIQGGGKVLAQAGNPVMDFELFCAKNMVAPITFYYPDKAEVTQSCLLLRAHLFVVNRHVAETEWTAFKLKDVRHERDTVVTRSVNRSGAETDLTFIKVTKGPLFKDNVNKFCSNKDDFPARNDAVTGIMNTGLAFVYSGNFLIGNQPVNTTTGACFNHCLHYRAQTRRGWCGSAVICNVNGKKAVYGMHSAGGGGLAAATIITRELIEAAEKSMLALEPQGAIVDISTGSVVHVPRKTKLRRTVAHDVFQPKFEPAVLSRYDPRTDKDVDVVAFSKHTTNMESLPPVFDIVCDEYANRVFTILGKDNGLLTVEQAVLGLPGMDPMEKDTSPGLPYTQQGLRRTDLLNFNTAKMTPQLDYAHSKLVLGVYDDVVYQSFLKDEIRPLEKIHEAKTRIVDVPPFAHCIWGRQLLGRFASKFQTKPGLELGSAIGTDPDVDWTPYAAELSGFNYVYDVDYSNFDASHSTAMFECLIKNFFTEQNGFDRRIAEYLRSLAVSRHAYEDRRVLIRGGLLSGCAATSMLNTIMNNVIIRAALYLTYSNFEFDDIKVLSYGDDLLIGTNYQIDFNLVKERLAPFGYKITPANKTTTFPLTSHLQDVTFLKRRFVRFNSYLFRPQMDAVNLKAMVSYCKPGTLKEKLMSIALLAVHSGPDIYDEIFLPFRNVGIVVPTYSSMLYRWLSLFR