Protein: Ubiquilin-1 (Protein linking IAP with cytoskeleton 1) (PLIC-1) (Q9JJP9)
Organism: Rattus norvegicus (Rat)
Function: Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome. Plays a role in the ERAD pathway via its interaction with ER-localized proteins UBXN4, VCP and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome. Plays a role in unfolded protein response (UPR) by attenuating the induction of UPR-inducible genes, DDTI3/CHOP, HSPA5 and PDIA2 during ER stress. Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion. Negatively regulates the TICAM1/TRIF-dependent toll-like receptor signaling pathway by decreasing the abundance of TICAM1 via the autophagic pathway. Promotes the ubiquitination and lysosomal degradation of ORAI1, consequently down-regulating the ORAI1-mediated Ca2+ mobilization (By similarity). Suppresses the maturation and proteasomal degradation of amyloid beta A4 protein (A4) by stimulating the lysine 63 (K63)-linked polyubiquitination. Delays the maturation of A4 by sequestering it in the Golgi apparatus and preventing its transport to the cell surface for subsequent processing. Promotes the surface expression of GABA-A receptors. Ubiquitinates BCL2L10 and thereby stabilizes protein abundance (By similarity).
Subcellular location: Nucleus {ECO:0000250|UniProtKB:Q9UMX0}. Cytoplasm {ECO:0000269|PubMed:11528422}. Endoplasmic reticulum {ECO:0000250|UniProtKB:Q9UMX0}. Cytoplasmic vesicle, autophagosome {ECO:0000250|UniProtKB:Q9UMX0}. Cell membrane {ECO:0000250|UniProtKB:Q9UMX0}. Note=Recruited to the ER during ER-associated protein degradation (ERAD). Colocalizes with PSEN1 in the cell membrane and in cytoplasmic juxtanuclear structures called aggresomes. Colocalizes with ORAI1 and TICAM1 in the autophagosome. Colocalizes with EPS15 and HGS in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments and with EPS15 also in aggresomes (By similarity). Detected in neuronal processes and synapses (PubMed:11528422). {ECO:0000250|UniProtKB:Q9UMX0, ECO:0000269|PubMed:11528422}.
Interaction partners:
- Ataxin-3
- Ubiquitin conjugation factor E4
- Leukocyte surface antigen CD47
- Proteasome
- Proteasomal ubiquitin receptor ADRM1
- Next to BRCA1 gene 1 protein
- Transitional endoplasmic reticulum ATPase
- Epidermal growth factor receptor pathway substrate 15
- 26S proteasome non-ATPase regulatory subunit 2
- 26S proteasome regulatory subunit 7
Sequence:
MAESAESGGPPGAQDSAADSGPAEPKIMKVTVKTPKEKEEFAVPENSSVQQFKEEISKRFKSHIDQLVLIFAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQDNSAQQTNTTGNSVTSSPAPDSNPTSGPAANSSFGLGGLGGLAGLSSLGLNTTNFSELQSQMQRQLLSNPEMMVQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHMLNNPNIMRQTLELARNPAMMQEMMRNQERDLSNLESIPGGYNALRRMYTDIQEPMLNAAQEQFGGNPFASLVSSPSSAEGTQPSRTENRDPLPNPWAPQTPQSSPASGSTGSTTNTVSTSAGNATSTPAGQGTSGPNLVPGAGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMMQSLSQNPDLAAQMMLNNPLFAGNPQLQEQMRQQLPTFLQQMQNPDTLSAMSNPRAMQALLQIQQGLQTLATEAPGLIPGFTPGLAAGNSGGPAGTTAPSTAPGEDTNPQGGAAEPGHQQFIQQMLQALAGVNPQLQSPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPS