Protein: Kinesin-like protein Klp61F (Bipolar kinesin KRP-130) (P46863)
Organism: Drosophila melanogaster (Fruit fly)
Function: Important role in mitotic dividing cells. Microtubule motor required for spindle body separation. Slow plus-end directed microtubule motor capable of cross-linking and sliding apart antiparallel microtubules, thereby pushing apart the associated spindle poles during spindle assembly and function. Forms cross-links between microtubules within interpolar microtubule bundles. Contributes to the length of the metaphase spindle, maintains the prometaphase spindle by antagonizing Ncd, drives anaphase B, and also contributes to normal chromosome congression, kinetochore spacing, and anaphase A rates. Displays microtubule-stimulated ATPase activity. Required for normal fusome organization. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface.
Subcellular location: Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:10469596, ECO:0000269|PubMed:19800237, ECO:0000269|PubMed:8589456, ECO:0000269|PubMed:9885249}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:8589456}. Cytoplasm {ECO:0000269|PubMed:10469596, ECO:0000269|PubMed:9885249}. Note=In somatic cells, cytoplasmic during interphase, localized to centrosomal asters at the onset of mitosis in prophase and associated with spindle structures during the remainder of mitosis (PubMed:10469596). In male and female germ cells, associates with fusomes during interphase, then localizes to centrosomal asters during prophase and to spindles in metaphase (PubMed:10469596). Coincident with spindle microtubules from prophase to metaphase and, as mitosis proceeds, found both at the spindle poles and along the spindle fibers (PubMed:8589456). {ECO:0000269|PubMed:10469596, ECO:0000269|PubMed:8589456}.
Interaction partners:
- Kinesin-like protein at 67A, isoform A
- Protein claret segregational
- Kinesin-like protein
- Protein abnormal spindle
- Structural maintenance of chromosomes protein
- Aurora kinase B
- Rac GTPase-activating protein 1
- Guanylate kinase-associated protein mars
- CENP-meta, isoform C
- Anillin
Sequence:
MDISGGNTSRQPQKKSNQNIQVYVRVRPLNSRERCIRSAEVVDVVGPREVVTRHTLDSKLTKKFTFDRSFGPESKQCDVYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGIIPRALSHLFDELRMMEVEYTMRISYLELYNEELCDLLSTDDTTKIRIFDDSTKKGSVIIQGLEEIPVHSKDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSIVVHIRENGIEGEDMLKIGKLNLVDLAGSENVSKAGNEKGIRVRETVNINQSLLTLGRVITALVDRAPHVPYRESKLTRLLQESLGGRTKTSIIATISPGHKDIEETLSTLEYAHRAKNIQNKPEVNQKLTKKTVLKEYTEEIDKLKRDLMAARDKNGIYLAEETYGEITLKLESQNRELNEKMLLLKALKDELQNKEKIFSEVSMSLVEKTQELKKTEENLLNTKGTLLLTKKVLTKTKRRYKEKKELVASHMKTEQVLTTQAQEILAAADLATDDTHQLHGTIERRRELDEKIRRSCDQFKDRMQDNLEMIGGSLNLYQDQQAALKEQLSQEMVNSSYVSQRLALNSSKSIEMLKEMCAQSLQDQTNLHNKLIGEVMKISDQHSQAFVAKLMEQMQQQQLLMSKEIQTNLQVIEENNQRHKAMLDSMQEKFATIIDSSLQSVEEHAKQMHKKLEQLGAMSLPDAEELQNLQEELANERALAQQEDALLESMMMQMEQIKNLRSKNSISMSVHLNKMEESRLTRNHRIDDIKSGIQDYQKLGIEASQSAQAELTSQMEAGMLCLDQGVANCSMLQVHMKNLNQKYEKETNENVGSVRVHHNQVEIICQESKQQLEAVQEKTEVNLEQMVDARQQLITEDRQRFIGHATVATDLVQESNRQFSEHAEHQRQQLQICEQELVRFQQSELKTYAPTGTTPSKRDFVYPRTLVATSPHQEIVRRYRQELDWSDLDTTATIDECSEGEHDVSMHSVQELSETETIMNSTPIEPVDGVTVKRGCGTTRNSNSNALKPPVATGGKRSSSLSRSLTPSKTSPRGSPAFVRHNKENVA