Protein: Patronin (Short spindle protein 4) (A1ZAU8)
Organism: Drosophila melanogaster (Fruit fly)
Function: Involved in mitotic spindle assembly. Regulates microtubule (MT) severing. Antagonizes the activity of the kinesin-13 depolymerase Klp10A thereby switching off the depolymerization of the MTs at their pole-associated minus ends, which turns off poleward flux and induces anaphase B spindle elongation. Involved in asymmetric cell division of sensory organ precursor (SOP) cells by playing a role in the asymmetric localization of Sara-expressing endosomes to the pIIa daughter cell but not to the pIIb cell. Klp98A targets Sara-expressing endosomes to the central spindle which is symmetrically arranged in early cell division. During late cytokinesis, central spindle asymmetry is generated by enrichment of Patronin on the pIIb side which protects microtubules from depolymerization by Klp10A while unprotected microtubules on the pIIa side are disassembled by Klp10A, leading to the asymmetric delivery of Sara-expressing endosomes to the pIIa daughter cell.
Subcellular location: Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body {ECO:0000269|PubMed:24100293}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:20946984}. Cytoplasm, cytoskeleton, microtubule organizing center {ECO:0000269|PubMed:32066907}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:32066907}. Note=Localizes to centrosomes in prophase, to the midbody during cytokinesis, diffusely throughout the metaphase spindle and to punctae in interphase that often overlap with MTs (PubMed:20946984). Colocalizes with Sas-4 and SAK at the microtubule organizing center (MTOC) (PubMed:20946984). In the fat body, localizes to a perinuclear non-centrosomal microtubule-organizing centers (ncMTOCs) (PubMed:32066907). {ECO:0000269|PubMed:20946984, ECO:0000269|PubMed:32066907}.
Interaction partners:
- Short stop, isoform H
- Kinesin-like protein Klp10A
- Spastin
- Katanin p80 WD40 repeat-containing subunit B1
Sequence:
MDVETQEIRQARQRASVKWLLSKAFNNRVPDNLKEPFYRDHENQERLKPQIIVELGNATLYCQTLANLYSDPNYQSMNHWSIIQTLARKGVPVAESADMPITETVLIQTNPLRINAHMSVIESLMVLYAKEISSGDRVMAAIRRISGNNYQAPTGQSYEQALLGWISHACAALKKRIIKEVDAGLPDDNGSRLQTPDIPPVRDFQDLCDGICLALLISYYCPKVVPWTSVRINYLPAVEDSIHNILLVCNFSQKHLPYTVMHMTPEDVTYMRGSMKLNLVVLLTDLFNLFEIHPAKCVCYPGMDGQVPHSNSFGGGLNRRSTPPNEYQTVQSNNFDGNHAEAFVVHKSRGITTLASMHSQQQQQLHQQQQHQQQYHQQPLQQHPSQSQLQIQQQEPLVPARLRQAKEKTNVESKADERGRRSRRNSSSEDSQLTIENFGGSQDQLNTLGRYERDRERKLSNTSVGSYPVEPAVAVRSSIADARGTLQLGYDTDSGSEKQDRETEKYSMRRQVSVDNVPTVSSHNLSNAGSPLPVARHKQHSSDKDYSSNSGMTPDAYNDSRSTSGYDPESTPVRKSSTSSMPASPAAWQLDVGDDDMRSLENASKLSTIRMKLEEKRRRIEQDKRKIEMALLRHQEKEDLESCPDVMKWETMSNESKRTPDMDPVDLDKYQQSIAIMNMNLQDIQQDIHRLATQQSQMQAQHLQAQQLMQAQQIANMLNQAYNAPVSAYSSRPPSRDPYQQQLHHQQQQPMPMPQPMQYVNEHGQYMSPPQPAHYMPQQAQQPQSIYSDNGAAYNHSNHSPYGGTPQYRSSVVYDDYGQPTNHFYLHESSPQPQAHQHPQRRTWAHSAAAAAYEQQQQIQPSLVDVNAWQTQQHQKQKQTWMNRPPSSAGAPSPGSFMLHQNGGGGGGGGGGGELQHLFQVQASPQHGQRQVSGSNGVQRQQSLTNLRDNRSPKAPQNMGMPMGMPMQQEDMMAPQSICFIGDEEDVDELERNIIESMQSTHISDFVHQQQQQHQHQQQLQQQQRLQGHSGRGSSSEDYDSGEMISNKLNITSGNLTYRIPSPSRPSIQANSFQDPRAMAAASGGEDQPPEKGFYISFDDEQPKRPKPPLRAKRSPKKESPPGSRDSVDNQATLKRESLSHLHNNNNIGFGNDDVNSKPVTRHSIHGLNNSNSVKSPGNATYNKYTDEPPIQLRQLAVSGAMSPTSNERHHLDDVSNQSPQQTQQPMSPTRLQQSSNNAEAAKNKALVIGADSTNLDPESVDEMERRKEKIMLLSLQRRQQQEEAKARKEIEASQKREKEREKEEERARKKEEQMARRAAILEQHRLKKAIEEAEREGKTLDRPDLHVKLQSHSSTSTTPRLRQQRTTRPRPKTIHVDDASVDISEASSISSRGKKGSSSNLTGYGQLSSNSMKRDYYRGSQDSLTVKESPDDYPSTSSTPIGRRGSYKTSREPAGVERGRTLSRISVAKGSTLNFRGRKSNSLMNLCGPKLYKQPAAKSNRGIILNAVEYCVFPGVVNREAKQKVLEKIARSEAKHFLVLFRDAGCQFRALYSYQPETDQVTKLYGTGPSQVEEVMFDKFFKYNSGGKCFSQVHTKHLTVTIDAFTIHNSLWQGKRVQLPSKKDMALVI