Protein: Platelet adherence protein A (Adhesin PadA) (A8AZP4)
Organism: Streptococcus gordonii (strain Challis / ATCC 35105 / BCRC 15272 / CH1 / DL1 / V288)
Function: A cell wall protein involved with Hsa in host cell interactions required for colonization and pathogenesis. Involved in recognition of platelets. Interacts with human platelet integrin receptor GPIIbIIIa (a complex of ITGA2B and ITGB3). Involved in platelet spreading, presumably by activation of outside-in signaling leading to platelet activation and then spreading. Spreading also involves GPIIbIIIa. Binding to platelets under static conditions causes platelet dense granules to secrete ADP (similar to release induced by fibrinogen binding), has no effect on platelet alpha granule release. The N-terminal 656 aa residue fragment (called F2) also binds platelets, causes dense granule secretion and allows platelet spreading. Acts in concert with Hsa to promote binding to human fibronectin (FN1) and vitronectin (VTN), and biofilm formation. F2 bind activated platelets more strongly than unactivated platelets. Binding to both FN1 and VTN is mediated at least in part by their glycosylation.
Subcellular location: Secreted, cell wall {ECO:0000255|PROSITE-ProRule:PRU00477, ECO:0000269|PubMed:19884334, ECO:0000269|PubMed:27616700}; Peptidoglycan-anchor {ECO:0000255|PROSITE-ProRule:PRU00477}.
Interaction partners:
- Transport protein ComB
- LPXTG cell wall surface protein
- Efflux transporter, RND family, MFP subunit subfamily
- ATP-binding cassette transporter-like protein
- Surface-associated protein CshB
- Surface-associated protein CshA
- LPXTG cell wall surface protein, collagen binding domain
- CAAX amino terminal protease family
- RADC-like protein
- Novel STAND NTPase 3 domain-containing protein
Sequence:
MKDFLKKVLILFTVLLMSMPSSVLNLGTSVVRADDPLNIETRRIDEHTTITQNGCYRKIEKTDATDWTVPRKPIDLVILQDASGSFRTTIPSVKNALKRLTTYVSPEQYDENDPHLVKTDDPRTTDRVFVASYQGLDQVRYFENNDFSGNPANVYTDANSTGKNYTYGNSGLTSDQNKVHNFIDNIAVDGGTPTVPAIDDTIAQYNRVKGNMENGRKTVFLLVTDGVANGYRLPGTNTVVMDKSWTRTDAIQKAWRVDSYPEAAQDIIGRANELKAAGNQLKAAVGSEGSVVVGFWERVDNFTEKYYQYGPAYLNGFGNTINIGDNRSVQAIFHDALQSMASPDKVVNGKNVSFYVNEQNNIDVFSQKILESVAAALVKDDITGEFDITEGYKVDAIRINGKKIVPKVTDPSKEIRGTITQTGNKVKISVPDSVFNPGKNSFDYDLSKEARAPETDEDSEVDPPENYVPEKEEITVPELTGKFKAGDFETRQIGGRNQTVEVQKLEYCYPSATKTVKDADASNDIGVIPDPLELTKKPSYSAQLSKKDEEFTYTVDYNFNNVPYEFEKNVMLTDPIDYRLEVVSHSAQGPDGQSWPTRVVTQQDAGGNSQSVVVADVPPQGKDYNYLIMKKAKLKMTVRLKEEYRKNQASKAYLAILQNNNGYGLVNQGNIMWNGEDDSPNQDAHAKTKDKASTIRRSNPIYVKPPLDTEVDKKVNEKEHEGLQADGEEFEYKVTAPWPGIADKFTLTDTVVDELEIVPNSAKVTVAGKSYNALTKAISINGQTIDITLDKAQLTSLNRLISRRGGSEVQEIELIFKAKIRPGADLSKYKKNGAVNIPNTADVILNDKKKTSKEVTVTPPKPKEPTVSKKINNTLDSLVTFDGQPYTYNITTAVPSDVAGYKKFVISDKLDADLEFDGQASISGPLADVFEIQTNGQTVTATVKEGKFKELAKYSFVELTIPAKVKAGVTGKTIENKAKISFTNENNVAKEVESNPVTVTPPPVTKKINENLDHLDIATGQPYKYNVKTTLPSDITSYKEFVITDTLEDELSVINEGTDKPVISGPAAEFFDVTVSGQKVTATMKNFAGASALAGQEIELVIPAKINDGVTRSNIPNKATFSFKDKNDHKGEKETIPVTVTPPTEPNVSKKINGDQDNATIAAETDFTYNIKTTLPNDIDTYKSFAITDTLDENLGVVNPEPSISEEAKKFFDITVSGNTVTATMKDFAKASALANKEIELVIHAKVKKESVLPEIPNTAKITYTNKNNESKEKETEPVKVTPPPITKKVNGKDQEDLASLTSTFKYTVDSKVPIVADKFVLSDTLEEVLTFDGDATVTIDGQTVTDVTVAKKDQKLTVTFDKDQVKKYAGKAVQVAFDAKIKSGYTVDQLVAKYPNGDKAAIPNKASFVVNDNPETEKFSNPVTVTPPPPNTPEIEKKVNGADSYNLQTRLEEFTYSLNTAMPTNATEFTVTDELKSVLEFAGKKGDVQVKIDGKAANDQATISTDKNTLTVAFAEKAVKANAGKSIEVTFKAKIREGANLLDYLVPGQGIRIPNKASYDIDHNPKFHKDSNEVPVTPPSPEQPPIEKDVNDKAEATLEARDEEFTYHVKTKIPYEATAFNITDTLKEVLDFSGEKGQAEATVDGKKLSDDHIAINGQTITVTLNQEELKANADKEIKLTFKAKIRPNANLAAYVVGDKVVINNQASYNVDLPDNPGVHKDSNIVPVTPPSPEKPEIEKTVNDAKEATLANRDEIFTYKVKTKVPFDATAFSIDDTIKDVLEFADAGSATLNGEALEADRISIADQKITLTLTEDQVKNNGGKEVVLTFKAKIRQGANLSGYIEKGKTVINNQASYNAAFPNDPNFHKDSNIVPVTPPNPENPPIEKKVNEAESANLGARDEEFTYTIDTTVPLDVTGFAVYDTIEKVLEFSGENGQASATVDGQPLDASHITIKGQKITVKLTEDEAKALGGKAVHVSFKAKIKAGANLSDYIEKDGTTRIYNTAKYNFNNDPGTEQSSKPVPVIPPTPTEPELKKEVNGKEAETLANRDDVFTYTVKTTVPQDATAFSISDSLVPVLEFAGEDAEASLTLNGEKLDAKQIKLKDQTISAELTEAQVKANGGKEVVLNFKAKIREGANLADYIEADGVTRVPNKASYVANFPHRPKVEKDSNIVPVTPPSPENPPVEKKVNNKPSATLDSRDEEFTYTIDTKVPVDATGFKITDELKDVLEFSGKKGQAEVTVDGDKDVIEDSQITVDKQVLTVTLTKDQVKKYGNKAVHVSFKAKIRKNVSLAGYIEADGVTRIPNIAKYIINDDPKTEKSTEPVPVIPPSPEEPGIKKEVNGQPEATLKERYEEFTYKVTTSVPQDATAFSVSDTLVPVLEFSGEKGQATATLDGQEIDANRINVADQTISMALTEDEVKANGGKEVTLTFKAKIREGANLSAYIEKGKTSIPNTASYTAGFPNRPEIHKDSNRVPVTPPTPEEPEIKKDVNGKEEETLANRNDEFTYHINTKVPFDATAFSINDELKDVLEFADGTGRATASLNGQALDADRISINGQTITVNLTEEQVKNNGGKDVNLTFTAKIRQGVNLSGYIKDGKTSIPNKASYRVDFPNNPGVTKDSNEVPVTPPSPENPPIEKKVNEAESANLGARDEEFTYTIDTTVPLDVTGFAVYDTIEKVLEFSGENGQASATVDGQPLDASHITIKGQKITVKLTEDEAKALGGKAVHVSFKAKIKAGANLSDYIEKDGTTRIYNTAKYNFNNDPGTEQSSKPVPVIPPTPTEPELKKEVNGKEAETLANRDDVFTYTVKTTVPQDATAFSISDKLEDVLEFAGESSATLAGEDLKADQITTDGQIIKLTLTEDQVKANGGKEVVLNFKAKIREGANLSAYMKADKAEVPNKASYTVGFPNKPAVTKDSNEVPVTPPSPEQPPIEKDVNSKPSETIADRTEEFTYNIHTTMPQDATGFTVTDELKDVLEFAGDVQVTLGGKKADAAVAKNGQTLEVTFPEETVKANGGKKVQVTFKAKIKADADLTPYETANSYSVPNTASYLINNNPTSKKETKPVTVEVPKQPGPEVTKKINRTLDHLDVDRDVPYMYNVNTQIPKDIRLYKEFTVTDTLEPVLEITGTPVAYVDGYATDAVETKVEGNTVTVTVKDFARISGYKEIQLYIPAKLKADSDLSAYENQTVPNKATIAFKDSNGKNGTKESNPVTVRPRDPEKPEEPKPNEPAKTVGPADGSNPSTAYRLKELKEGFRFDVTAKVPTDPVDESGNPIKDAQGRDVKTELNSFTVTDELEKVLKVDRVAVKVEENKVAEAIAKITAKIEKAESDLKELEGKETNGTFAKKLAEAEKKVEELTAQLAAAKEKAAAAPATPAPASDSDAGNATATPAPADNNAEVAALEESLKAAQAELEQLKADGAKAGNLATPEEQKVEQDKLNKNLEQLKESKEKLEKALEAFTTVNDKGEITDEALAKIAKVTVEGQKVTVEVTDKAVLEALKGSTFRVIIYSSIKDGADLSSYLNKENNETKIPNKATVTFNDKPKVTNTVNVYPPEPTTPPQTPPHTPPTTPGTPPPTTPDTPPAPKGDLPPAPTPEPEKPKNILPKTGTSATMVNEVIIGMILVLMGLLLRRKPKH