Protein: 1-phosphatidylinositol 3-phosphate 5-kinase FAB1 (Phosphatidylinositol 3-phosphate 5-kinase) (EC 2.7.1.150) (Formation of aploid and binucleate cells protein 1) (Styryl dye vacuolar localization protein 7) (Type III PIP kinase) (PIPkin-III) (P34756)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. The product of the reaction, PI(3,5)P2 is an important regulator of vacuole homeostasis perhaps by controlling membrane flux to and/or from the vacuole. PI(3,5)P2 regulates the transition between trans-SNARE complex formation and vacuole membrane fusion. Hyperosmotic shock-induced increase in the levels of PtdIns(3,5)P2 requires the presence of VAC7, VAC14, and/or FIG4.
Subcellular location: Vacuole membrane {ECO:0000269|PubMed:11889142, ECO:0000269|PubMed:18653468, ECO:0000269|PubMed:19037259, ECO:0000269|PubMed:9763421}; Peripheral membrane protein {ECO:0000269|PubMed:18653468, ECO:0000269|PubMed:19037259}. Endosome membrane {ECO:0000269|PubMed:11889142, ECO:0000269|PubMed:9763421}; Peripheral membrane protein {ECO:0000269|PubMed:11889142, ECO:0000269|PubMed:9763421}. Note=Localization to the vacuole membrane requires VAC14 and FIG4. {ECO:0000269|PubMed:18653468, ECO:0000269|PubMed:19037259}.
Interaction partners:
- Vacuole morphology and inheritance protein 14
- Vacuolar segregation protein 7
- Polyphosphoinositide phosphatase
- Autophagy-related protein 18
- Phosphatidylinositol 3-kinase VPS34
- Phosphatidylinositol 4-kinase PIK1
- Phosphatidylinositol 4-kinase STT4
- Serine/threonine-protein kinase VPS15
- Phosphatidylinositol 4-kinase LSB6
- Vacuolar segregation protein PEP7
Sequence:
MSSEEPHASISFPDGSHVRSSSTGTSSVNTIDATLSRPNYIKKPSLHIMSTSTTSTTTDLVTNPILSNISVPKISPPTSSSIATATSTSHVTGTASHSNIKANANTSTSVNKKNLPPTTSGRIPSSTIKRYPSRYKPSHSLQLPIKNDSNFKRSSIYASKSTVTAIPIRNNRPISMQNSYARTPDSDHDDVGDEVSSIKSASSSLTASLSKSFLFAFYNNRKKDKTSNNGVLSKEYWMKDESSKECFSCGKTFNTFRRKHHCRICGQIFCSSCTLLIDGDRFGCHAKMRVCYNCYEHADTYEDSSDEENDSTMQLNEPRSRSRSRSSNTNPYSHSHSHLHLISQDNHNGTDLHDPVAATDNPQQQNEVYLLNDDDVQSIMTSGEDSKLFISTPPPPPKMAIPATKQGGSLEISFDSENDRALHYQDDNPGRHHHLDSVPTRYTIRDMDNISHYDTNSNSTLRPHYNTNNSTITINNLNNTTSNNSNYNNTNSNSNINNPAHSLRRSIFHYVSSNSVNKDSNNSSATPASSAQSSSILDPANRIIGNYAHRNYKFKFNYNSKGPSQQNDTANGNNDNNNNNNNNNNNNNNNSASGIADNNNIPSNDNGTTFTLDKKKRNPLTKSKSTSAYLEYPLNEEDSSEDEGSMSIYSVLNDDHKTDNPIRSMRNSTKSFQRAQASLQRMRFRRKSKSKHFPNNSKSSIYRDLNFLTNSTPNLLSVVSDDNLYDDSSPLQDKASSSAASRLTDRKFSNSSGSNNNSNSNSNINTDPWKRIASISGFKLKKEKKRELNEVSLLHMHALLKQLLNDQEISNLQEWITLLDGALRKVLRTILNARDLNTLDFRQTYVKIKRISGGSPQNSEYIDGVVFSKALPSKTMPRHLKNPRILLIMFPLEYQKNNNHFLSIESVFRQEREYLDKLVSRLKSLHPDIIYVGANVSGYALELLNDSGIVVQFNMKPQVIERIAKLTEADIAISVDKLATNIKMGECETFEVKSYIYGNISKTYTFLRGCNPELGGTILLRGDSLENLRKIKQVSEFMVYAIFSLKLESSFFNDNFIQLSTDVYLKRAESKKLQVFEGYFADFLIKFNNRILTVSPTVDFPIPFLLEKARGLEKKLIERINQYESESDLDRQTQLNMLQGLESTITKKHLGNLIKFLHEMEIENLELEFQKRSRQWEVSYSSSQNLLGTGSHQSITVLYSMVSTKTATPCVGPQIVTIDYFWDSDISIGQFIENVVGTARYPCQQGCNGLYLDHYRSYVHGSGKVDVLIEKFQTRLPKLKDIILTWSYCKKCGTSTPILQISEKTWNHSFGKYLEVMFWSYKDSVTGIGKCPHDFTKDHVKYFGYNDLVVRLEYSDLEVHELITPPRKIKWKPHIDIKLKVELYYKILEKINNFYGSVLSRLERIKLDSMTKDKVLSGQAKIIELKSNATEEQKLMLQDLDTFYADSPCDQHLPLNLVIKSLYDKAVNWNSTFAIFAKSYLPSETDISRITAKQLKKLFYDSSRKDSEDKKSLHDEKAKTRKPEKNELPLEGLKDVEKPKIDSKNTTENRDRTNEPQNAVTITTFKDDTPIIPTSGTSHLTVTPSASSVSSSLTPQTEERPPISRSGTGISMTHDKSTRPNIRKMSSDSSLCGLASLANEYSKNNKVSKLATFFDQMHFDALSKEFELERERERLQLNKDKYQAIRLQTSTPIVEIYKNVKDAVDEPLHSRSSGNNLSSANVKTLEAPVGEHSRANNCNPPNLDQNLETELENSISQWGENILNPSGKTTASTHLNSKPVVKETSENPKSIVRESDNSKSEPLPPVITTTTVNKVESTPQPEKSLLMKTLSNFWADRSAYLWKPLVYPTCPSEHIFTDSDVIIREDEPSSLIAFCLSTSDYRNKMMNLNVQQQQQQQTAEAAPAKTGGNSGGTTQTGDPSVNISPSVSTTSHNKGRDSEISSLVTTKEGLLNTPPIEGARDRTPQESQTHSQANLDTLQELEKIMTKKTATHLRYQFEEGLTVMSCKIFFTEHFDVFRKICDCQENFIQSLSRCVKWDSNGGKSGSGFLKTLDDRFIIKELSHAELEAFIKFAPSYFEYMAQAMFHDLPTTLAKVFGFYQIQVKSSISSSKSYKMDVIIMENLFYEKKTTRIFDLKGSMRNRHVEQTGKANEVLLDENMVEYIYESPIHVREYDKKLLRASVWNDTLFLAKMNVMDYSLVIGIDNEGYTLTVGIIDFIRTFTWDKKLESWVKEKGLVGGASVIKQPTVVTPRQYKKRFREAMERYILMVPDPWYREGN