Protein: Highly reducing polyketide synthase ZEA2 (EC 2.3.1.-) (Polyketide synthase 4) (PKS4) (Zearalenone biosynthesis polyketide synthase 2) (A0A098D8A0)
Organism: Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) (Wheat head blight fungus) (Fusarium graminearum)
Function: Highly reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of zearalenone (ZEA), a nonsteroid estrogen that is a contaminant of cereal grains and causes estrogenic disorders in humans and animals. The ZEA backbone is synthesized from a single acetyl-CoA molecule and eight malonyl-CoA molecules. The reducing polyketide synthase ZEA2 is proposed to synthesize a reduced hexaketide intermediate by using different combinations of its reductive domains during each round of condensation. The hexaketide thioester is then transacylated to the non-reducing polyketide synthase ZEA1 and is further condensed with three malonyl-CoAs without reductive tailoring to yield a mixed reduced/unreduced nonaketide. ZEA1 must be able to interact with ZEA2 to facilitate starter-unit acyltransfer and initiate polyketide biosynthesis. ZEA1 also mediates the required C2-C7 cyclization to form the resorcylate core and catalyzes the formation of the macrolactone. ZEB1 is then responsible for the chemical conversion of beta-zearalenonol (beta-ZOL) to ZEA in the biosynthetic pathway.
Interaction partners:
Sequence:
MSVDNKQVPGPVAIVGLACRFPGDATSPSKFWDLLKSGKDAYSETTDRYNAQAFYHPNSKRQNVLPVTGGHFLKQDPHVFDAAFFNITAAEAISLDPKQRIALEVAYEAFENAGKPLKQVAGTTTACFVGSSMSDYRDAVVRDFAHNPKYHVLGTCEEMIANRISHFFDIHGPSATVHTACSSSLVAIHLACQSLLSGDAEMALAGGVGMILTPDGTMQLNNLGFLNPEGHSRSFDKDAGGYGRGEGCGILVLKKLDKAIQDGDNIRAVIRASGVNSDGWTQGVTMPSSEAQAALIKHVYETRGLDYGATQYVEAHGTGTKAGDPVETGAIHRTIGQGASKNRKLWVGSVKPNIGHLEAAAGVASVIKGVLAMENSLIPPNIHFASPNPEIPLDEWNMAVPTKLTPWPAARTKRMSVSGFGMGGTNGHVVLEAFNSTPQSILYGDAQYQPAHNGKRLFTFSSHDQAGLDRVSKSLVDHLDSLGPAGARPEYLADLGYSLSVGKSGLSWKTAHLAESLTELREKLSSPQSEHAVREPRSQPKIGFVFTGQGAQWARMGVEMLHRPVFKESVQRSTDYLQQLGCDWTPIVELSRAQKESRLTLPEISQPICSVLQIALVDELRSWGVAPVSVVGHSSGEIAAAYCIEALSHKDAIAVAYFRGKVSAGLNHLNGGMMAVGCSRAEAETLIDESDLQGGHVTVACVNSPSNVTLSGDVAPLDQLKGILEKRGIFARRLRVEVAYHSTHMNSVFADYTASIADIEPQSCPSHQPIMVSSVTNNQVDPALLGSYYWGRNLISPVLFSDTIKEMVSPADGNGQKAVDLLVEIGPHGALGGPIEQILSHFDIENVGYQSMLTRGQNAVETSLELATSLFLQGVAIDIQKVNGDSGCRLLTNLPPYPWNHSKKFRAESRLQRELIAQSTPTRSIIGAPVPKMNESQRVWRGFIRLDDEPWIRGHTVGTTVLFPGAGMVSIVLEAAQQMVDPGKVARAFRLRDVSFSAAMALPEDQATEVIIQMKPQLVATSGSTPATWWEFTVSSCAGTDQLRDNCRGLITIDYEGNTSQQMAHEDSQVVSGRISDYHQILEECPATYAKDRFYKHMMKAAWRYGETFQGVENCHPGDGKTVFDVKLIDIGETFSKGQLDRPFLIHGATLDAVFQGWLGSTYKNGTFEFDKPFVPTKIGEMEISFNVPSEAGYMMPGLCRSHRSGFNELSADTIMFDKDLSRVILSVIDFRTSELEMDGAATEETTVEVDPADITSKVLWDYSLSLMEPCDLKQVMGSIVAQNSLTDFVRMLLHDNPAANIVEFISRSDGLPNTYASKLPPGTILPTQIRYAVVDETEDVGDENAASSMLTIDALVDSVSASGATADIVVIPQGFQFQDNYAKILEPLAKVSKPNTTIVVAVDTPDTTVPLKAKGFQLLHSIQGTPSLEVFAGLTGEQEKPTNGIHKEEVVLLLPSMLSTVTKEFAEEVQLDLEGQGFSVSTESLAESIDDSTFDGKTCVSLLEVERPLLDSLSESDFQLIRKVVLTSQRILWVTHGESPSLALVDGFSRCIMSEIEGVKFQVLHLSEPTGLQHGPRLAAKVIASKASDNEFRDKDGLLQVARIFKGLTENENIRHHLHDDVRVTRLSNQEHPLRLTIGKPGLLDTLYFVDDERVLAPLADHEVEIQVKATGLNFRDVMASMALVPVKGLGQEASGIVLRTGRDATHLKPGDRVSTLDMGTHATVMRADHRVTVKIPDAMSFEEAAAVPVVHTTAYYALVRLAKLQRGQSVLIHAAAGGVGQAALQLANHLGLVVYATVGSDDKRKLLTDTYQVSEDHIFNSRDASFAKGIMRVTGGRGVDCVLNSLSGELLRVSWSCLATFGTFVEIGLRDITNNMLLDMRPFSKSTTFSFINMYTLFEEDPSALGDILEEVFKLLGGGILQTPSPMTVYPINQVEDAFRIMQQGKHRGKIVLSFPDDAQAPVLHVAKNSMKLDSQATYLFVGGLGGLGRSLAKEFVSCGAKNIAFISRSGDSTSEAKATIKEITSRGANVKAYAADISNETAFLNAMKECSREFPPIKGVVQMAMVLRDVVFEKMTYEEWKLPLKPKVQGSWNLHKYFDHERPLDFMVICSSSSGIYGYPSQAQYAAGNTYQDALAHYRRAQGLRAVSVNLGIMRDVGVLAEQGTSGNIKLWEEVLGIREPAFHALMKSLIKGQTDNNSEFPAQICTGLGTADIMATHGLAKPTYFQDPRFGPLAVTSLSSDASGDKQSTAMSISSQLSEASSKAKATEIITNALIGKVADILQMPQSEVDPGQPLYRYGVDSLVALEVRNWITREMKVNVALLEILAAVPMESFAGKLASTSKLVTVS