Protein: Lysine-specific demethylase JMJ30 (AtJMJ30) (EC 1.14.11.27) (EC 1.14.11.68) (EC 1.14.11.69) (JmjC domain-containing protein 30) (Jumonji domain-containing protein 5) (AtJMJD5) ([histone H3]-trimethyl-L-lysine(36) monodemethylase JMJ30) (Q8RWR1)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Histone demethylase that demethylates 'Lys-36' (H3K36me) of histone H3 with a specific activity for H3K36me3 and H3K36me2. Also active on 'Lys-27' (H3K27me) of histone H3 with a specific activity for H3K27me3 and H3K27me2. No activity on H3K36me1 and H3K27me1. Involved in the control of flowering time by demethylating H3K36me2 at the FT locus and repressing its expression. Acts within the central clock and contributes, in parallel with LUX, to temperature compensation, probably as a component of the evening complex, to maintain circadian period at increasing temperatures; this mechanism involves binding to and regulation of CCA1 and PRR7 promoters. Works in concert with TOC1 to promote the morning-phased clock genes CCA1 and LHY which function as components of the central oscillator. Together with JMJ32, regulates the flowering-repressor FLOWERING LOCUS C (FLC) locus by removing the repressive histone modification H3 lysine 27 trimethylation (H3K27me3), especially at elevated temperatures (e.g. 29 degrees Celsius), thus preventing extreme precocious flowering. JMJ30 and JMJ32 are regulators involved in the integration of abscisic acid (ABA) and brassinosteroids (BR) signaling pathways. Together with JMJ32, controls ABA-mediated growth arrest during the post-germination stage in unfavorable conditions, and responses to ABA during root development, via the removal of repressive histone mark (H3K27me3) from the SnRK2.8 promoter, thus promoting SnRK2.8 expression and subsequent kinase-dependent ABI3 activation. In addition, removes the repressive histone marks (H3K27me3) from the BZR1 locus in response to stress and ABA, thus activating the BR signaling pathway which, in turn, inhibits the ABA signaling pathway. Able to drive tissue identity changes to promote callus formation form somatic cells via a massive genome-wide chromatin remodeling (e.g. H3K9me3 demethylation) leading to the induction of Lateral organ Boundaries-Domain (LBD) genes (e.g. LBD16 and LBD29) that establish root primordia; when in complex with ARF proteins (e.g. ARF7 and ARF19), recruits ATXR2 which promotes the deposition of H3K36me3 at LBD genes promoters, thus ensuring their stable activation during callus formation.
Subcellular location: Nucleus {ECO:0000269|PubMed:25267112, ECO:0000305|PubMed:21139085}. Cytoplasm {ECO:0000269|PubMed:25267112, ECO:0000305|PubMed:21139085}. Endoplasmic reticulum {ECO:0000269|PubMed:25267112}. Note=Predominantly localized in the nucleus, especially in euchromatin. {ECO:0000269|PubMed:25267112}.
Interaction partners:
- Lysine-specific demethylase ELF6
- Probable transcription factor PosF21
- Lysine-specific demethylase REF6
- Two-component response regulator-like APRR1
- Histone-lysine N-methyltransferase ATXR2
- Protein EARLY FLOWERING 4
- Protein LHY
- Protein CCA1
- Lysine-specific demethylase JMJ18
- Histone H3-like 4
Sequence:
MSGATTASSGDHNNLRLPTPTLDAESQTLLQSISAEGGYAYARMAVLAVAGDQSAAEAARDMAWEQLHSGPWHSVLPVWRDAYSMACLHVAKIHFAAGEFGEALGALDMGLIMGGMLLRKDLHDSVLLVSSEARKMTKSLEEASGDFKGERLVPEVPVDVNEVRHVLANLQLLVLKILPCRSLTCKRVEKRSGLSLEGFLRDYYLPGTPVVITNSMAHWPARTKWNHLDYLNAVAGNRTVPVEVGKNYLCSDWKQELVTFSKFLERMRTNKSSPMEPTYLAQHPLFDQINELRDDICIPDYCFVGGGELQSLNAWFGPAGTVTPLHHDPHHNILAQVVGKKYIRLYPSFLQDELYPYSETMLCNSSQVDLDNIDETEFPKAMELEFMDCILEEGEMLYIPPKWWHYVRSLTMSLSVSFWWSNEAESSSS