Protein: Myosin-VIIa (DmVIIa) (Protein crinkled) (Q9V3Z6)
Organism: Drosophila melanogaster (Fruit fly)
Function: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements: can function in cells as a single-molecule cargo transporter. A very slow and high-duty-ratio motor, may be suitable for tension maintenance of actin filaments. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. Plays a key role in the formation of cellular projections and other actin-based functions required for embryonic and larval viability. Necessary for auditory transduction: plays a role in Johnston's organ organization by functioning in scolopidial apical attachment and therefore to acoustic stimulus propagation from the antenna a2/a3 joint to transducing elements. Interaction with the myosin zip may be important for its function in scolopidial apical attachment. During oogenesis it has Cad99c-dependent and Cad99c-independent roles in regulating the shape and spacing of the follicle cell microvilli which secrete eggshell material such as the vitelline membrane. May be required for the normal expression of Cad99c in the follicle cell microvilli.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:15886106, ECO:0000269|PubMed:25236597}. Cytoplasm, cell cortex {ECO:0000269|PubMed:25236597}. Cell projection, microvillus {ECO:0000269|PubMed:25236597}. Note=In scolopale cells (radially organized units in the Johnston's organ), protein is concentrated along actin-rich scolopale rods and more apically near scolopale cell-cap cell junctions (PubMed:15886106). In neurons, protein is concentrated near the basal body where neurons are tethered to the scolopale cell (PubMed:15886106). In germline and associated somatic cells of the ovary, it has a punctate distribution and associates with specific F-actin cell structures (PubMed:25236597). In germline cells, expressed in the F-actin-rich cytocortex of the oocyte (from stage 3, peaking at stages 9 to 10a) and in the cortex of nurse cells (from stage 6) (PubMed:25236597). Expressed in the microvilli and terminal web of follicle cells (PubMed:25236597). High expression at the base of the follicle cell microvilli in the brush border (PubMed:25236597). {ECO:0000269|PubMed:15886106, ECO:0000269|PubMed:25236597}.
Interaction partners:
- Uncharacterized protein, isoform D
- Cadherin-99C
- Calmodulin
- Dyschronic, isoform G
- Calcium and integrin-binding family member 2
- Calmodulin-related protein 97A
- LD20463p
- Myosin-2 essential light chain
- Dilute class unconventional myosin, isoform C
- Myosin heavy chain 95F
Sequence:
MVIVTRGDYIWIEPASGREFDVAIGARVVSAEGRRIQVRDDDGDEVWLAPERRIKAMHASSVQGVEDMISLGDLHEAGILRNLLIRYKENLIYTYTGSILVAVNPYQILPIYTGDQIKLYKERKIGELPPHIFAIGDNAYAHMKRYRQDQCIVISGESGAGKTESTKLILQYLAAISGKHSWIEQQILEANPILEAFGNAKTIRNDNSSRFGKYIDIHFSANGVIEGAKIEQYLLEKSRIVSQNHSERNYHVFYCILAGLSADEKSRLDLGMAADYKYLTGGNSITCEGRDDAAEFSDIRSAMKVLLFSDQEIWEIIKLLAALLHCGNIKYKATVVDNLDATEIPEHINVERVAGLLGLPIQPLIDALTRRTLFAHGETVVSTLSRDQSVDVRDAFVKGIYGRMFVHIVRKINTAIFKPRGTSRNAIGVLDIFGFENFDQNSFEQFCINYANENLQQFFVQHIFKLEQEEYNHEAINWQHIEFVDNQDALDLIAIKQLNIMALIDEEARFPKGTDQTMLAKLHKTHGSHKNYLKPKSDINTSFGLNHFAGVVFYDTRGFLDKNRDTFSPDLLHLVSQSTNKFLRQIFAQDIEMGAETRKRTPTLSTQFRKSLDALMKTLSSCQPFFIRCIKPNELKKPMMFDRGLCCRQLRYSGMMETIRIRRAGYPIRHGFREFVERYRFLIPGVPPAHRTDCQAATSRICAVVLGKSDYQLGHTKVFLKDAHDLFLEQERDRVLTRKILILQRSIRGWVYRRRFLRLRAAAITVQRFWKGYAQRKRYRNMRVGYMRLQALIRSRVLSHRFRHLRGHIVGLQAHARGYLVRREYGHKMWAVIKIQSHVRRMIAMRRYRKLRLEHKQFAEVLQLRKLEEQELLHRGNKHAREIAEQHYRDRLHELERREIQEQLENRRRVEVNMNIINDAARKQEEPVDDGKLVEAMFDFLPDSSSDAPTPHGGRETSVFNDLPHAQNVNQDDIIAPIHISEDEEDLSEFKFQKFAATYFQGNVNHQYAKKALKHPLLPLHTQGDQLAAQALWITILRFTGDMPEPKYHTMDRMDTTSVMSKVTATLGRNFIRSKEFQEAQLMGLDPDAFLKQKPRSIRHKLVSLTLKRKNKLGEDVRRRLQDDEYTADSYQSWLQSRPTSNLEKLHFIIGHGILRAELRDEIYCQICKQLTNNPLKSSHARGWILLSLCVGCFAPSEKFVNYLRAFIREGPPGYAPYCEERLKRTFNNGTRNQPPSWLELQATKSKKPIMLPITFMDGNTKTLLADSATTARELCNQLSDKISLKDQFGFSLYIALFDKVSSLGSGGDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEIFAPWHEPTHDQVATNLIYQQVVRGVKFGEYRCDKEEDLAMIAAQQYFIEYSTDMSMERLFTLLPNFIPDFCLSGVDKAIERWAALVLQAYKKSYYVKDKIAPLKIKEDIVSYAKYKWPLLFSRFYEAYRNSGPNLPKNDVIIAVNWTGVYVVDDQEQVLLELSFPEITAVSSQKTNKVFTQTFSLSTVRGEEFTFQSPNAEDIRDLVVYFLDGLKKRSKYVIALQDYRAPSDGTSFLSFFKGDLIILEDESCGESVLNNGWCIGRCDRSQERGDFPAETVYVLPTLSKPPQDILALFNIEEAHHGRRLSMASNGGAVEPRDRPHTLMEYALDHFRLPPKRTMSKTLTLSSKRSEELWRYSRDPIKAPLLRKLQSKEEFAEEACFAFAAILKYMGDLPSKRPRMGNEITDHIFDGPLKHEILRDEIYCQLMKQLTDNRNRMSEERGWELMWLATGLFACSQGLLKELLLFLRTRRHPISQDSMHRLQKTIRHGQRKYPPHQVEVEAIQHKTTQIFHKVYFPDDTDEAFEVDSSTRAKDFCNNISQRLSLRTSEGFSLFVKIADKVISVPEGDFFFDFVRHLTDWIKKARPIRDGANPQFTYQVFFMKKLWTNTVPGKDRNADLIFHYHQELPKLLRGYHKCSREEAAKLAALVFRVRFGENKQELQAIPQMLRELIPSDIMKIQSTSEWKRSIVASYNQDGGMTSEDAKVAFLKIVYRWPTFGSAFFEVKQTTEPNYPEMLLIAINKHGVSLIHPVTKDILVTHPFTRISNWSSGNTYFHMTIGNLVRGSKLLCETSLGYKMDDLLTSYISLMLTNMNKNRTIRAN