Protein: Autophagy-related protein 2 (Q21480)
Organism: Caenorhabditis elegans
Function: Lipid transfer protein involved in autophagosome assembly and in the distribution of atg-9 and atg-13 during the autophagy-mediated degradation of protein aggregates. Tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion (By similarity). Binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source to the IM for membrane expansion (By similarity). Involved in autophagy-mediated degradation of ribosomal RNA and ribosomal proteins in lysosomes, which is essential for maintaining nucleotide homeostasis.
Subcellular location: Preautophagosomal structure membrane {ECO:0000250|UniProtKB:Q96BY7}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q96BY7}. Lipid droplet {ECO:0000250|UniProtKB:Q96BY7}. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P53855}; Peripheral membrane protein {ECO:0000250|UniProtKB:P53855}. Cytoplasm {ECO:0000305|PubMed:21802374}.
Interaction partners:
- Autophagy-related protein 18
- Serine/threonine-protein kinase unc-51
- Autophagy-related protein 9
- Ectopic P granules protein 6
- Autophagy-related protein 5
- Ubiquitin-like protein atg-12
- Ubiquitin-like modifier-activating enzyme ATG7
- Ubiquitin-like-conjugating enzyme ATG3
- non-specific serine/threonine protein kinase
- Phosphatidylinositol 3-kinase catalytic subunit type 3
Sequence:
MTLSTFNRVWCKVMLQRYMGAWLDNNLSVDQLSLELANGCLELDNLDINTKAVSNGFLQCNIPLKLIDGYLGKIKIEIPWLSLMTDPTRMCIEDLQLTFRGAEVMKINDIETLTSMIESVLMGLSTDDMARSVYEEVSKENNVASELLGPDDTADSFSGFIDAVYSRFCLKIKHLTLRFETDPKNRSTMATALEIRVETITFMDEQMRSCEMDHTNATDLVTTQPHGVVSTTNLRKNLTFSGVTFHTDVFSQINNDGMGDEENVLITSMHIRREKAKQMSPTKSVQNSLHPEMFQSAMSDMDAFHSCYDKLTQDYCSPDQLETLRTAPAEPELFSAPIQCAEVVGDISCVFRIKNGANDVNYADVDESKVETDVFIKGINVFATPSQIEIVNRFFSSIVTPKELVVHEQGKPMSKEDYENMTKNMEPNQSNEPPMAGASFGGNWNVGEVFREFDDLKSIKEKEKSEKEKFKSLKASGNIKEEFTVNTHIGTVLMYIPHCDYMSSDYAQQHGGYANVLNYLKKESESFFKSIQGYSFMSKHGLANIRQQSDSFYPKDHLRIVGGSLGITSSCKRVGNVDSFLCRVVATHFDMLEYLTPESAPENSTPIRIGLLDYSEQENLESDPNFKMVLSTSSEQKGLTKVDILLGAVKTELDFSIIDRISNLIACRPFFDEALTNYGNRNTVPQLKDDLYSDVLIAEDDVKSKTLVNLKCSNWQVDLRIPKADLRDPTGSRLPFSQRHVHNEFLRLGIKEIDVSIPIEKSVTLIEILCTEMIGDFCGEGLNIPKQQQRILHASKNGFDKINLKLTLCSDAKKLPGCGRKTSTSGIPDSMMKSVSADIMMAHPKKEGPFSKVPRSYCSHDGEETEEIIQAGTRKEILDFQEECQNFASTFMLFTVPVLKLHIPEKNFLEILYNRLVNDLALFQPAAPAFRNNQSTNSNVQPLESFQECVSPKNYAESEHSDLDDDVATLHESIESLNFDRDIPHTFVMTLNANKCTVLCNTAIKEAEKQPESSQVSLDLEKVHIGTTAGYHGDINHTYFHFTSSKAAAGSIDSPRAPRIPNLISAKDFGKWTKDCNQLEHVPITDELSSGSTEDAFAVALHMHFRPDVNMKDVLLGIALRNSQLQAKPFRHWGAFWITQLADLFTLQDYAIPGYELPSVSTDLHICFENAIIGYDHAWVNPNSKLKLRATLGQCNLASSIVSDMNISKTLCIFESCRLYMSNDLTKDAVRFEGYGTQKVSPKKFIPFLDLGSVQLDILFAVGDETGLRTNPTFEIRCQNDIINAWACADSLATFMKTVMEYTSHEQIPIKTPEEESQELMKSTKTDDINKSVAGESVWSDASTGSKHIQKMTVGANLPDDVEKRIQAMIQEAVNENDERDGIAIGEDAVREFATIQPKHDANETNESFEHVASRNFSVTDDEFCMVDDNIFGSGITIQPGESRTRPMPAKQLPEEPLINGSEFFQTIEDSGSDALYQTMSGQINPVLRYFLKDVTVRLSLYAGNDLSTSPSPIKTYCTEEYRNGFGPEQKIEQNSTGGPNRDHSAFVVFELSKITYLKQIFDKTAPMLSTTLFQVNDIVIKDCVRASDIKEMLYQYSVTNQPRRATAPIVTVRMSETHSKEGKMRVSMLPIKINVDQDTLEFLTDFFEETSKLLDLPKNQMSMPLIQRPVIEVPADIGSKKTTPKTSVSSSEGDIARMYPSIPSPSLTLEPLRPSPVQPPTPLGDLTYLEKISSNHQSPVKRPIIDAPLTASAVSIGKIFERDAHSDEDEEEIIDPIQMAAALEIRELNEELGRIEQLEKKKTDDLFKQSYSSSSSETESESSAPQSSQVRRLHESLDASNPTGLSDLTGDWADDHDLTYTHHDVVRQESPSFNCNISPIKGYSPPAELRPLNLMDSETERDSSASPVTSSPIKPARNIKKELPPLKMPRQPLSNDDILMRSTMMGSVHPSQSVHNLVDTSDDLEEHGNFLDSIDNEDDNEKQKIEEEMEEDEKEEEEERNKEIQEAVERGETFFKQFVFSPSVNIYVDYQGKRKITMEKAGALVGLLMAFGQLNQMPINLRKIDTRTGLLGTGRCMQHAIGEWSGDLLTNMPSVIASYGPISPLVQIGRGVVDLFLMPVAEFRKDDGNVMKGVQRGVGSFSVSSAAGIVGMAQTVTGFVQSLAEMTMKEIKPDDPSTRRVARRYNRNHGMNPTDVRHSLQLAYGILYDGYHQTRDDLELAAQEDRASGNSVVRSAFRYAVPTFLGPIVMATQVTYQLLGGLRNQLRPDTYQDERRKWGEKDVPGGVNK