Protein: Intermembrane lipid transfer protein VPS13 (Suppression of the onset of impotence protein 1) (Vacuolar protein sorting-associated protein 13) (Vacuolar protein-targeting protein 2) (Q07878)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Mediates the transfer of lipids between membranes at organelle contact sites. Binds phospholipids, including phosphatidic acid (PA), phosphorylated phosphatidylinositol (PI), phosphatidylcholine (PC), phosphatidylethanolamine (PE), ceramide, and phosphatidylserine (PS). Involved in mitochondrial lipid homeostasis, in a MCP1-dependent manner. Involved in cortical reticulophagy (also known as cortical endoplasmic reticulum (ER) macroautophagy), acting at the late endosome to package ER into autophagosomes. Involved in the formation of the prospore membrane during sporulation, in a SPO71-dependent manner. Involved in Golgi vesicle transport, in a CDC31-dependent and ARF1-dependent manner. Plays a role in actin cytoskeleton organization.
Subcellular location: Endosome membrane {ECO:0000269|PubMed:27280386, ECO:0000269|PubMed:28334785, ECO:0000269|PubMed:28864540, ECO:0000269|PubMed:30018089, ECO:0000269|PubMed:32690699, ECO:0000305|PubMed:22442115, ECO:0000305|PubMed:24036347}; Peripheral membrane protein {ECO:0000269|PubMed:28122955}. Mitochondrion outer membrane {ECO:0000269|PubMed:26370498, ECO:0000269|PubMed:27280386, ECO:0000269|PubMed:30018089, ECO:0000269|PubMed:34830155}; Peripheral membrane protein {ECO:0000269|PubMed:28122955}. Prospore membrane {ECO:0000269|PubMed:22442115, ECO:0000269|PubMed:24036347, ECO:0000269|PubMed:27280386}; Peripheral membrane protein {ECO:0000269|PubMed:28122955}. Vacuole membrane {ECO:0000269|PubMed:26370498, ECO:0000269|PubMed:27280386, ECO:0000269|PubMed:30018089, ECO:0000269|PubMed:32690699}; Peripheral membrane protein {ECO:0000269|PubMed:28122955}. Nucleus outer membrane {ECO:0000269|PubMed:26370498, ECO:0000269|PubMed:27280386, ECO:0000269|PubMed:30018089}; Peripheral membrane protein {ECO:0000269|PubMed:28122955}. Peroxisome {ECO:0000269|PubMed:28864540}. Golgi apparatus, trans-Golgi network membrane {ECO:0000269|PubMed:34830155}; Peripheral membrane protein {ECO:0000269|PubMed:28122955}. Note=Found at different interorganellar contact sites depending on the growth state of the cell (PubMed:26370498, PubMed:27280386, PubMed:30018089, PubMed:32690699). Localizes from endosomes to the vacuolar membrane during starvation (PubMed:32690699). Localizes to mitochondrial-endosome contacts (PubMed:27280386). Localizes to nuclear envelope-vacuole contact sites (nuclear-vacuole junction; NVJ) during respiration (PubMed:26370498, PubMed:27280386, PubMed:30018089). {ECO:0000269|PubMed:26370498, ECO:0000269|PubMed:27280386, ECO:0000269|PubMed:30018089, ECO:0000269|PubMed:32690699}.
Interaction partners:
- Prospore membrane adapter protein SPO71
- Mitochondrial adapter protein MCP1
- Endosomal/vacuolar adapter protein YPT35
- Autophagy-related protein 2
- Mitochondrial distribution and morphology protein 10
- Probable ATP-dependent permease
- Vacuolar protein sorting-associated protein 35
- Sporulation-specific protein 73
- Mitochondrial distribution and morphology protein 12
- Membrane-anchored lipid-binding protein LAM6
Sequence:
MLESLAANLLNRLLGSYVENFDPNQLNVGIWSGDVKLKNLKLRKDCLDSLNLPIDVKSGILGDLVLTVPWSSLKNKPVKIIIEDCYLLCSPRSEDHENDEEMIKRAFRLKMRKVSEWELTNQARILSTQSENKTSSSSSEKNNAGFMQSLTTKIIDNLQVTIKNIHLRYEDMDGIFTTGPSSVGLTLNELSAVSTDSNWAPSFIDITQNITHKLLTLNSLCLYWNTDSPPLISDDDQDRSLENFVRGFKDMIASKNSTAPKHQYILKPVSGLGKLSINKLGSTEEQPHIDLQMFYDEFGLELDDTEYNDILHVLSSIQLRQITKKFKKARPSFAVSENPTEWFKYIAACVINEIHEKNKMWTWESMKEKCEQRRLYTKLWVEKLKLKNLEAPLRDPIQEAQLSELHKDLTYDEIILFRSVAKRQYAQYKLGMTEDSPTPTASSNIEPQTSNKSATKNNGSWLSSWWNGKPTEEVDEDLIMTEEQRQELYDAIEFDENEDKGPVLQVPRERVELRVTSLLKKGSFTIRKKKQNLNLGSIIFENCKVDFAQRPDSFLSSFQLNKFSLEDGSPNALYKHIISVRNSSKDQSSIDNHATGEEEEEDEPLLRASFELNPLDGLADSNLNIKLLGMTVFYHVHFITEVHKFFKASNQHMETIGNIVNAAEATVEGWTTQTRMGIESLLEDHKTVNVSLDLQAPLIILPLDPHDWDTPCAIIDAGHMSILSDLVPKEKIKEIKELSPEEYDKIDGNEINRLMFDRFQILSQDTQIFVGPDIQSTIGKINTASSTNDFRILDKMKLELTVDLSILPKAYKLPTIRVFGHLPRLSLSINDIQYKTIMNLIANSIPSMIDDEENNGDYVNYSSGSEKEMKKQIQLQLKNTLKALENMQPLQIEQKFLELHFDIDQAKIAFFQCIKNDSRNSEKLVDILCQRLNFNFDKRAKEMNLDLRVHSLDVEDYIELTDNKEFKNLISSGVEKVTRSQKDLFTLKYKRVQRIVPHNDTLIELFDQDIVMHMSELQLVLTPRSVLTLMNYAMLTFTDPNAPEMPADVLRHNKEDRDDAPQKINMKIKMEAVNVIFNDDSIKLATLVLSAGEFTMVLLPERYNINLKLGGLELTDETNESFSRDSVFRKIIQMKGQELVELSYESFDPATNTKDYDSFLKYSTGSMHVNFIESAVNRMVNFFAKFQKSKVSFDRARLAAYNQAPSIDAVNNMKMDIVIKAPIIQFPKLVGTQENNYDTMRFYLGEFFIENKFSVIDESHKINHIKLGVREGQLSSNLNFDGSSQQLYLVENIGLLFNIDRDPLPQDDTPELKVTSNFESFALDLTENQLTYLLEISNKVSSAFNITDENSGESGGKGEIKSPSPDPASLSSESERTATPQSLQGSNKSNIKNPEQKYLDFSFKAPKIALTLYNKTKGVTSLNDCGLTRIMFQDIGCSLGLKNDGTVDGQAHVAAFRIEDVRNIKDNKHTELIPKSKNKEYQFVANISRKNLEVGRLLNISMTMDSPKMILAMDYLVSLKEFFDAIMSKSHENNLYYPENTNQKPENKAIVESVQEGGDVTKIQYSVNIIETALILLADPCDMNSEAISFKIGQFLVTDQNIMTVAANNVGIFLFKMNSSEEKLRLLDDFSSSLTIDKRNSTPQTLMTNIQLSVQPLLMRISLRDIRLAMLIFKRVTTLLNKMTEKEDNGEEEESTDKIQFSHEFERKLAVLDPSILGERSRASQSSDSESIEVPTAILKNETFNADLGGLRFILIGDVHEMPILDMNVNEITASAKDWSTDFEALASLETYVNIFNYSRSSWEPLLEMIPITFHLSKGHSEMDPAFSFDILTQRIAEITLSARSIAMLSHIPASLTEELPLASRVSQKPYQLVNDTELDFDVWIQDKTTEDNKNEVVLLKANTSLPWEFEDWRSIREKLDIDKSKNILGVCVSGQNYKTIMNIDATTEGENLHVLSPPRNNVHNRIVCEARCDENNVKIITFRSTLVIENTTSTEIELLVDSKDPNKPSLKYAIKPHQSKSVPVEYAYDSDIRIRPASEDIYDWSQQTLSWKSLLSNQMSIFCSSKEDSNQRFHFEIGAKYDEREPLAKIFPHMKIVVSASMTIENLLPADINFSIFDKREEKRTDFLKTGESMEVHHISLDSFLLMSVQPLQDEASASKPSIVNTPHKSPLNPEDSLSLTLSGGQNLLLKLDYKNIDGTRSKVIRIYSPYIIMNSTDRELYIQSSLLNIAQSKILLENEKRYTIPKMFSFDKEDDKSNRARIRFKESEWSSKLSFDAIGQSFDASVRIKNKEQESNLGINISEGKGKYLLSKVIEIAPRYIISNTLDIPIEVCETGSMDVQQIESNITKPLYRMRNIVDKQLVLKFLGGDSNWSQPFFIKNVGVTYLKVLKNSRHKLLKIEILLDKATIFIRIKDGGDRWPFSIRNFSDHDFIFYQRDPRKVSDPYKDDQSNESSSRSFKPIFYRIPSKSIMPYAWDFPTAKEKYLVLESGTRTREVRLAEIGELPPLRLDKRSKDKPAPIVGLHVVADDDMQALVIVNYKANVGLYKLKTASATTTSSVSVNSSVTDGFVQKDEDEKVNTQIVVSFKGVGISLINGRLQELLYINMRGIELRYNESKAYQTFSWKMKWMQIDNQLFSGNYSNILYPTEIPYTEKEIENHPVISGSISKVNDSLQAVPYFKHVTLLIQEFSIQLDEDMLYAMMDFIKFPGSPWIMDSRDYKYDEEIQLPDVSELKTAGDIYFEIFHIQPTVLHLSFIRSDEISPGLAEETEESFSSSLYYVHMFAMTLGNINEAPVKVNSLFMDNVRVPLPILMDHIERHYTTQFVYQIHKILGSADCFGNPVGLFNTISSGVWDLFYEPYQGYMMNDRPQEIGIHLAKGGLSFAKKTVFGLSDSMSKFTGSMAKGLSVTQDLEFQRVRRLQQRINKNNRNALANSAQSFASTLGSGLSGIALDPYKAMQKEGAAGFLKGLGKGIVGLPTKTAIGFLDLTSNLSQGVKSTTTVLDMQKGCRVRLPRYVDHDQIIKPYDLREAQGQYWLKTVNGGVFMNDEYLSHVILPGKELAVIVSMQHIAEVQMATQELMWSTGYPSIQGITLERSGLQIKLKSQSEYFIPISDPEERRSLYRNIAIAVREYNKYCEAIL