Protein: 16S rRNA endonuclease CdiA (rRNase CdiA) (EC 3.1.-.-) (CdiA-ECL) (Toxin CdiA) (D5CBA0)
Organism: Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCTC 10005 / WDCM 00083 / NCDC 279-56)
Function: Toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. CDI modules allow bacteria to communicate with and inhibit the growth of closely related neighboring bacteria in a contact-dependent fashion; upon forced induction decreases E.cloacae target cell counts about 20-fold, about 100-fold in E.coli. Intracellular expression of CdiA-CT (residues 3087-3321) inhibits E.coli cell growth when induced, but coexpression with its cognate immunity protein CdiI allows cell growth. Cleaves 16S rRNA in vivo and in vitro between adenine 1493 and guanosine 1494 of E.coli 16S rRNA. Inhibition of 16S rRNA cleavage is specific to the cognate immunity protein, non-cognate CdiI from E.chrysanthemi strain EC16 does not inhibit this protein. Purified CdiA-CT inhibits E.coli cell growth when added to cultures but not when added as a complex with cognate CdiI, suggesting cognate CdiI prevents import into the target cell. CdiA-CT (without CdiI) is probably imported in an F-pilus-mediated fashion, although it is not clear if this is physiologically significant. Gains access to the cytoplasm of target cells by using integral inner membrane protein FtsH.
Subcellular location: Target cell, target cell cytoplasm {ECO:0000269|PubMed:24657090, ECO:0000269|PubMed:24889811, ECO:0000269|PubMed:25174572}. Note=Secreted to the cell surface by CdiB, its two partner secretion pathway (TPS) partner (Probable). Toxin translocation into the target cell depends on the proton motive force of the target cell, but not on tolA or tonB. {ECO:0000269|PubMed:25174572, ECO:0000305}.
Interaction partners:
- TPS family two-partner secretion family member CdiB
- GMP synthase
- Tail spike TSP1/Gp66 N-terminal domain-containing protein
- Thioesterase 1/protease 1/lysophospholipase L1
- Tail spike TSP1/Gp66 N-terminal domain-containing protein
- Rhamnogalacturonase A/B/Epimerase-like pectate lyase domain-containing protein
- Inosine-5'-monophosphate dehydrogenase
- chitinase
Sequence:
MMKQDQVRFSQRALSALLSVLLATQPLLPAVAASITPSGNTQMDKAANGVPVVNIATPNQSGISHNKYNDYNVGKEGLILNNATGQLNQTQLGGLIQNNPNLKAGQEAKGIINEVTGANRSNLQGYTEVAGKAANVIVANPYGITCNGCGFINTPNVTLTTGKPVLDASGKLQSLDVTQGAVTIEGAGLNGSQSDAVSIISRATEINVQLHAKDLRVVAGANRVAADGSVSALKGEGTAPKVAVDTGALGGMYANRIRLVSSETGVGVNLGNLNARQGDIALSSAGKVVLKNTLASGSTTVSAADVTLRGDHKAGGNVTVSGQTALTLDQAHVAADNNLQLTTRGTLTQNGGAFTAANDATLAATTLIQSVDAQASAGRHLAVNAEKNAALNGSVVAGQQLSVKGGELVQQGNLSASEIALNAQTLTQESRSTTNASGNITLTTSGHSQLKGSTTAGQSLAVSAGSLANHGALAAVADTRINTGIFSNTGTVQGNSLTVSGTDITSSGALKSASTLDIRADNATLSGETGAKGKTTVTASGNLNNSGTLISDDTLTLNAAQIVNSGTLSGVRGLTTSGKTFTASATSVTQSDGDVALNNTDTTLAGETSAGGAVTVQGRSLNTTATAQTQGNSVGVAVQNAKLEGTQAAKGNMTLKADSSLNHTGKSSASGLKVETGHLSNSGTLTASALVIDSPEVINGGLIHAGQTLSLVTRLLDNRSSGVLYSPSALSLSLSELNNAGIITSDAALSLSGSNLTNSGELSGTSLAIDYETLKNSAEGMLLAQGANRITAQSVSSAGSMVGNTLTLNADRLESAGLLQGDSALSLTAGILNLLTGSRTLTGGALGLSGTTLTTAGQLQGQDVSIRSHDWTNRGSSLATGSLDVTTAGTLSNTGELMSQGNGTLNAVTTVNSGNMLSAGDLSLNGKTLRNSGTLQGNRVTAHQDTITNSGTLTGIAALMLAARLEMAAPLLTLVNDASGSLLTAGELSVTGGDLRNAGQWQGKRVLIHAQALTNGGAIQAENLLDAQIDSTLTGTAGSKITSNGELALSALTLANSGQWIAKHLTLGASTLNNSGEITGVVALSVALTQLNNQAGGKLLSAGALTLDVENATNAGQIQGKATTVTAGQLINSGRLQGEALTLNASGALNNTASGVLLSENALTVSTATLNNQGTLQGGGESSVKATTRVQNDGKMLSGGKLTLTAPELANSSSGLVQAVRLLLDVVKAVNGGNVLATTRAELRGSSLDNSGTLQGADLQANYQSVTNSGTVLGTTSLTINGDALDNTESGKLYSGDKLLLDVRNYSGRGDVVSLGDTTLKLVNALVNTGTLAASKTLSVSSQNAMTNSGVMQGNAIALSAGGAFTNNGTLTTGNGSSTFNAQSLLLNASGSLQAGGDVQLTSRENITVNGFTGTAGSLTMTAAGTLLNTALIYAGNNISLFAARIHNIYGDILADNSLWMQKNAVGEANAEVVNRSGTIETTRGDITVNTGHLLNEADGLTVSQSEREYPDAIPAADEHYFSYDLNGRRSDFVLLLEDWKNDGSKVVYDWYEQCLGSGANGSGQCRDRVDYRLTGEDIRQFLLSESVVSVSATGSSARIAAGRDITINAGTLDNRASHILAGRNAVLAGGTLNNLSAEGGRRVTYVQAEYRCEWFYRDCSDSKWEPLTQYPDGSWGWFDEDYGWYGWVPYILGERTTEFVADGGVYRSVISAGGNVSANFTSDISNTNVTANSGEFSNTIDAPTLNTLSPEAIGKGLNSESLAQGGSADIRFPEQLGNITDALKDISGGSSLSDQNGSSGNYPLPSGNNGYFVPSTDPDSPYLITVNPKLDELGNMDDSLFNGLYDLLGITPGATPRETNSAYTDRNQFLGSSYFLDRLGLNPDRDYRFLGDAAFDTRYVSNAILNQTGSRYINGIGSDLDQMRYLMDSAAEQQKTLGLKFGVALTAEQVAALDKSMLWWESATINGQTVMIPKVYLSPKDVTVHSGSVISGNNVQLAGGNVINSGSTIAAQNGLSIDSSNSLSNLNAGLLSAGGGLNLSALGDINNIGSTISGKTVGLESVAGSINNITRAQQWNVDAGNVHFSGTDVGKTASITATDGLTMRAGQDINVTGANVSAGGSLGMAAGNDINITANEIVTSEGRAGRNRATTETASVTHQGSTLSAGDDLTLQAGNDVNARAAAIAAEGDVGIQAGRDVDLLAEASMERSSSQAKKKTAIDESVRQQGTEIASGGNTVILAGRDVTAQAADVTAQGDIGVAAGRDVNLTTATESDYRYREQTKTSSGFLSKKTTHTIEEESATREKGSLLSGDNVTVSAGNNLRVLGSAVAGDGDVALSAGNNVDIVAATNTDTAWRFKETKKSGLMGTGGIGFTIGSSKSTHDLREQGTTQSESFSTVGSTGGNVSIAAGKQAHIGGADIIAQKDISLTGDSVVIEPGHDKRTRDEKFEQKSSGLTVALSGAAGSAVNNAVTTAQSAKQSSDSRLAALQGTQAALSGVQAGQAVALDQVKGDSDKRNNNTIGVSASIGSQSSKSSSHMESETTTGSTLSAGNNVTIKATGSDITVAGSQIKAGKDVTLDAARDVNLIASQDTQQTTGKNSSSGGSLGVGVGVGSGGAGISISANANSSKGHEKGNGVWQNETTVDAGNRVTINTGRDATIAGAQVSGETVVADIGRDLTIASTQDSDHYNSKQNSVSGGAGYTFGAGGFSGSINVSRDKMTSDYDSVQEQSGLFAGNGGFDVTVGNHTQLDSGVIASTATADKNRLDTGTLGFSDIHNQADFKTEHQGAGISSGGSIGKQFAGNMANALLAGGGNSGHAEGTTQAAVSEGTLIIRDKENQKQDVADLSRDAEHANGSISPIFDKEKEQQRLQEVQLIGEIGSQVVDIANTQGEINGLNAGRKELADKGITEPGADASDEVKAAYQNALRETDAYKTTTAKYGTGSDLQRGIQAATAALQGLAGSDLTAALAGASAPELAYRIGHGMGIDNNTAAKTIAHAILGGAVAALQGNSAAAGAAGAATGELAAKAIAGMLYPDVKDLSTLSEEQKQTVSALATISAGMAGGLAGDSTGSAVAGGQAGKNAAENNSLALVARGCAVAAPCRTKVAEQLLEIGAKAGIAGLAGAAVKDMADKMTSDELEHLVTLEMMGNDEIIAKYVSLLHDKYAPSHTGGNLLPETLPGHTGNNTGSVDTGPNHTGNTNRQNDSGSNNTGNTEGAPNTGGNTTITPIPNGPSKDDIAYLALKGKEAQEAASNLGFDRRIPPQKAPFNSHGQPVFYDGKNYITPDIDSHNVTNGWKMFNSKGKRIGTYDSGLNRIKD