Protein: Three-prime repair exonuclease 1 (EC 3.1.11.2) (3'-5' exonuclease TREX1) (Q91XB0)
Organism: Mus musculus (Mouse)
Function: Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Plays a key role in degradation of DNA fragments at cytosolic micronuclei arising from genome instability: its association with the endoplasmic reticulum membrane directs TREX1 to ruptured micronuclei, leading to micronuclear DNA degradation (By similarity). Micronuclear DNA degradation is required to limit CGAS activation and subsequent inflammation (By similarity). Unless degraded, these DNA fragments accumulate in the cytosol and activate the cGAS-STING innate immune signaling, leading to the production of type I interferon. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1 (By similarity). NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair (By similarity).
Subcellular location: Nucleus {ECO:0000269|PubMed:15254239, ECO:0000269|PubMed:18045533}. Cytoplasm, cytosol {ECO:0000269|PubMed:17355961, ECO:0000269|PubMed:18045533}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:18724932}; Peripheral membrane protein {ECO:0000269|PubMed:18724932}. Note=Retained in the cytoplasm through the C-terminal region (PubMed:18045533). Localization to the endoplasmic reticulum membrane is required to direct TREX1 to ruptured micronuclei (By similarity). In response to DNA damage, translocates to the nucleus where it is specifically recruited to replication foci (PubMed:18045533). Translocation to the nucleus also occurs during GZMA-mediated cell death (PubMed:18045533). {ECO:0000250|UniProtKB:Q9NSU2, ECO:0000269|PubMed:18045533}.
Interaction partners:
- Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
- Malectin
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A
- Cyclic GMP-AMP synthase
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TMEM258
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit KCP2
- Oligosaccharyltransferase complex subunit OSTC
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
Sequence:
MGSQTLPHGHMQTLIFLDLEATGLPSSRPEVTELCLLAVHRRALENTSISQGHPPPVPRPPRVVDKLSLCIAPGKACSPGASEITGLSKAELEVQGRQRFDDNLAILLRAFLQRQPQPCCLVAHNGDRYDFPLLQTELARLSTPSPLDGTFCVDSIAALKALEQASSPSGNGSRKSYSLGSIYTRLYWQAPTDSHTAEGDVLTLLSICQWKPQALLQWVDEHARPFSTVKPMYGTPATTGTTNLRPHAATATTPLATANGSPSNGRSRRPKSPPPEKVPEAPSQEGLLAPLSLLTLLTLAIATLYGLFLASPGQ