Protein: E3 ubiquitin-protein ligase HERC2 (EC 2.3.2.26) (HECT domain and RCC1-like domain-containing protein 2) (HECT-type E3 ubiquitin transferase HERC2) (Q4U2R1)
Organism: Mus musculus (Mouse)
Function: E3 ubiquitin-protein ligase that regulates ubiquitin-dependent retention of repair proteins on damaged chromosomes. Recruited to sites of DNA damage in response to ionizing radiation (IR) and facilitates the assembly of UBE2N and RNF8 promoting DNA damage-induced formation of 'Lys-63'-linked ubiquitin chains. Acts as a mediator of binding specificity between UBE2N and RNF8. Involved in the maintenance of RNF168 levels. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of XPA which influences the circadian oscillation of DNA excision repair activity. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway.
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:O95714}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:O95714}. Nucleus {ECO:0000250|UniProtKB:O95714}. Note=Recruited to sites of DNA damage in response to ionising radiation (IR) via its interaction with RNF8. May loose association with centrosomes during mitosis. {ECO:0000250|UniProtKB:O95714}.
Interaction partners:
- E3 ubiquitin-protein ligase RNF8
- Neuralized-like protein 4
- E3 SUMO-protein ligase PIAS4
- Breast cancer type 1 susceptibility protein homolog
- Myelin transcription factor 1
- ubiquitinyl hydrolase 1
- Dedicator of cytokinesis protein 10
- BRCA1-associated RING domain protein 1
- Ubiquitin-protein ligase E3A
- E3 ubiquitin-protein ligase RNF168
Sequence:
MPSESFCLAAQSRLDSKWLKTDIQLAFTRDGLCGLWNEMVKDGEIVYTGTELAQNRELPLRKDDGVDAQSGTKKEDLNDKEKKEEEETPAPVYRAKSILESWVWGRQPDVNELKECLSVLVKEQQALAVQSATTTLSALRLKQRLVILERYFIALNRTVFQENVKVKWKSSSISVPPTEKKSARPTGRGVEGLARVGSRAALSFAFAFLRRAWRSGEDADLCSELLQESLDALRALPEASLFDESTVSSVWLEVVERATRFLRSVVTGDVHGTPGTKGPGGVPLQDQHLALAILLELAVQRGTLSQMLSAILLLLQLWDSGAQETDNERSAQGTSAPLLPLLQRFQSIICSKDVPHTESDMHLLSGPLSPNESFLRYLTLPQDNELAIDLRQTAVVVMAHLDRLATPCMPPLCSSPTSHKGSLQEVIGWGLIGWKYYANVIGPIQCEGLASLGVMQVACAEKRFLILSRNGRVYTQAYNSDMLAPQLVQGLASRNIVKIAAHSDGHHYLALAATGEVYSWGCGDGGRLGHGDTVPLEEPKVISAFSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDEAIPMLVAGLKGLKVIDVACGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVIKVRCGSQFSIALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRVTKPEPTALPGLDSKHIVGIACGPAQSFAWSSCSEWSIGLRVPFVVDICSMTFEQLDLLLRQVSEGMDGTADWPPPQEKECMAVATLNLLRLQLHAAISHQVDPEFLGLGLGSVLLNSLKQTVVTLASSAGVLSTVQSAAQAVLQSGWSVLLPTAEERARALSALLPCTVSGNEVNISPGRRFMIDLLVGSLMADGGLESALNAAITAEIQDIEAKKEAQKEKEIDEQEASASTFHRSRTPLDKDLINTGIYESSGKQCLPLVQLIQQLLRNIASQTVARLKDVARRISSCLDFEQQSCERSASLDLLLRFQRLLISKLYPGEKIGPISDTSSPELMGVGSLLKKYTALVCTHIGDILPVAASIASSSWQHFAEVACVMEGDFTGVLLPELVVSIVLLLSKNASLMQEAGAIPLLGGLLEHLDRFNHLAPGKERDDHEELAWPGIMESFFTGQNCRNNEEVTLIRKADLENHNKDGGFWTVIDGKVYGIKDFQTQSLTGNSILAQFAGEDPVVALEAALQFEDTQESMHAFCVGQYLEPDQEVVTIPDLGSLSSPLIDTERNLGLLLGLHASYLAMSTPLSPVEVECAKWLQSSIFSGGLQTSQIHYSYNEEKDEDHCSSPGGTPISKSRLCSHRWALGDHSQAFLQAIADNNIQDYNVKDFLCQIERYCRQCHLTTPITFPPEHPVEEVGRLLLCCLLKHEDLGHVALSLVHVGTLGIEQVKHRTLPKSVVDVCRVVYQAKCSLIKTHQEQGRSYKEVCAPVIERLRFLFNELRPAVCSDLSIMSKFKLLGSLPRWRRIAQKIIRERRKKRVPKKPESIDSEEKIGNEESDLEEACVLPHSPINVDKRPISMKSPKDKWQPLLNTVTGVHKYKWLKQNVQGLYPQSALLNTIVEFALKEEPVDVEKMRKCLLKQLERAEVRLEGIDTILKLAAKSFLLPSVQYAMFCGWQRLIPEGIDIGEPLTDCLRDVDLIPPFNRMLLEVTFGKLYAWAVQNIRSVLMDASARFKELGIQPVPLQTITNENPAGPSLGTIPQARFLLVMLSMLTLQHGANNLDLLLNSGTLALTQTALRLIGPTCDSVEDDMNASARGASATVLEETRKETAPVQLPVSGPELAAMMKIGTRVMRGVDWKWGDQDGPPPGLGRVIGELGEDGWIRVQWDTGSTNSYRMGKEGKYDLKLVELPVSSQPSAEDSDTEDDSEAEQGERNIHPTAMMLTSVINLLQTLCLSVGVHADIMQSEATKTLCGLLRMLVESGTTDKPAPPDRLVAREQHRSWCTLGFVRSIALTPQACGALSSPRWITLLMKVVEGHAPFTAASLQRQILAVHLLQAVLPSWDKTERARDMKCLVEKLFGFLGSLLTTCSSDVPLLRESTLRKRRARPQASLTATHSSTLAEEVVGLLRTLHSLTQWNGLINKYINSQLCSVTQSYAGKTSERAQLEDYFPDSENLEVGGLMAVLAVIGGIDGRLRLGGQVMHDEFGEGTVTRITPKGRITVQFCDMRMCRVCPLNQLKPLPAVAFSVNNLPFTEPMLSVWAELVNLAGSKLEKHKTKKSAKPAFAGQVDLDLLRSQQLKLYILKAGRALLSHQDKLRQILSQPAVQGTGTLQTDDGAAASPDLGDMSPEGPQPPMILLQQLLSSATQPSPVKAIFDKQELEAAALALCQCLAVESTHPSSPGCEDCSSSEATTPVSVQHIHLARAKKRRQSPAPALPIVVQLMEMGFPRKNIEFALKSLTGTSGNASGLPGVEALVGWLLDHSDVQVTEFSDAETLSDEYSDEEVVEDVDDTPYPVAAGAVVTESQTYKKRADFLSNDDYAVYVRENVQVGMMVRCCRTYEEVCEGDVGKVIKLDRDGLHDLNVQCDWQQKGGTYWVRYIHVELIGYPPPSSSSHIKIGDKVRVKASVTTPKYKWGSVTHQSVGLVKAFSANGKDIIVDFPQQSHWTGLLSEMELVPSIHPGVTCDGCQTFPINGSRFKCRNCDDFDFCETCFKTKKHNTRHTFGRINEPGQSAVFCGRSGKQLKRCHSSQPGMLLDSWSRMVKSLNVSSSVNQASRLIDGSEPCWQSSGSQGKHWIRLEIFPDVLVHRLKMIVDPADSSYMPSLVVVSGGNSLNNLIELKTININQTDTTVPLLSDCAEYHRYIEIAIKQCRSSGIDCKIHGLILLGRIRAEEEDLAAVPFLASDNEEEEDDKGSTGSLIRKKTPGLESTATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTVEGKVYSCGEATNGRLGLGMSSGTVPIPRQITALSSYVVKKVAVHSGGRHATALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAALTSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGSDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDSGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTTVDVATPSVHEPVLFQTARDPLGASYLGVPSDADSSSSSNKISGANNCKPNRPSLAKILLSLEGNLAKQQALSHILTALQIMYARDAVVGALMPAGMLAPVECPSFSSSAPASDVSAMASPMHMEDSTLAADLEDRLSPNLWQEKREIVSSEDAVTPSAVTPSAPSASSRPFIPVTDDPGAASIIAETMTKTKEDVESQNKTSGPEPQSLDEFTSLLIPDDTRVVVELLKLSVCSRAGDKGREVLSAVLSGMGTAYPQVADMLLELCVTELEDVATDSQSGRLSSQPVVVESSHPYTDDTSTSGTVKIPGAEGLRVEFDRQCSTERRHDPLTVMDGVNRIVSVRSGREWSDWSSELRIPGDELKWKFISDGSVNGWGWRFTVYPIMPAAGPKDLLSDRCVLSCPSMDLVTCLLDFRLNLTSNRSIVPRLAASLAACAQLSALAASHRMWALQRLRRLLTTEFGQSININRLLGENDGESRALSFTGSALAALVKGLPEALQRQFEYEDPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCMASRVAVALDKRTPLPRLFLDEVAKKIRELMADSESMDVLHESHSIFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALATLRPVQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIKKVAVNSGGKHCLALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDVAAGGAHSACVTAAGDLYTWGKGRYGRLGHSDSEDQLKPKLVEALQGHRVIDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKVNRVACGSAHTLAWSTSKPASAGKLPAQVPMEYNHLQEIPIIALRNRLLLLHHISELFCPCIPMFDLEGSLDETGLGPSVGFDTLRGILISQGKEAAFRKVVQATMVRDRQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMSSFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEICEELQNGLTPLLIVTPNGRDESGANRDCYLLNPATRAPVHCSMFRFLGVLLGIAIRTGSPLSLNLAEPVWKQLAGMSLTIADLSEVDKDFIPGLMYIRDNEATSEEFEAMSLPFTVPSASGQDIQLSSKHTHITLDNRAEYVRLAINYRLHEFDEQVAAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGIEPSASLVQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVIQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKQVLEEKLKYAIHFCKSIDTDDYARIALTGEPAADDSSEDSDNEDADSFASDSTQDYLTGH