Protein: Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY (EC 2.4.1.255) (GDP-fucose protein O-fucosyltransferase SPINDLY) (EC 2.4.1.221) (Q96301)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Probable O-linked N-acetylglucosamine transferase (OGT) involved in various processes such as gibberellin (GA) signaling pathway and circadian clock. OGTs catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Probably acts by adding O-linked sugars to yet unknown proteins. Acts as a repressor of GA signaling pathway to inhibit hypocotyl elongation. Functions with GIGANTEA (GI) in pathways controlling flowering, circadian cotyledon movements and hypocotyl elongation. Acts as a light-regulated promoter of elongation via its interaction with GI. Acts as an activator of cytokinin signaling. Required with SEC for gamete and seed development. Its OGT activity has been proved in vitro but not in vivo. Possesses O-fucosyltransferase activity on specific serine and threonine residues. Mediates O-fucosylation of the DELLA protein RGA, a repressor of the GA signaling pathway. O-fucosylation enhances RGA activity by promoting RGA binding to key transcription factors in brassinosteroid and light-signaling pathways. Regulates root hair patterning upstream of the transcription factor WER, independently of DELLA proteins and GA signaling. Involved in abscisic acid (ABA) signaling partly through functional ABAR. Mediates O-fucosylation of CPN20 that may depress ABA responses during seed germination and seedling development. Involved in the modulation of the pace of the circadian clock by mediating O-fucosylation of APRR5, one of the core circadian clock components. O-fucosylation promotes APRR5 proteolysis.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:12068105, ECO:0000269|PubMed:31899321}. Nucleus {ECO:0000269|PubMed:12068105, ECO:0000269|PubMed:31899321}.
Interaction partners:
- Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SEC
- DELLA protein GAI
- Alpha/beta-Hydrolases superfamily protein
- Ubiquitin carboxyl-terminal hydrolase 2
- Protein GIGANTEA
- DELLA protein RGA
- Ubiquitin carboxyl-terminal hydrolase
- DELLA protein RGL2
- DELLA protein RGL1
- Transcription factor TCP14
Sequence:
MVGLEDDTERERSPVVENGFSNGSRSSSSSAGVLSPSRKVTQGNDTLSYANILRARNKFADALALYEAMLEKDSKNVEAHIGKGICLQTQNKGNLAFDCFSEAIRLDPHNACALTHCGILHKEEGRLVEAAESYQKALMADASYKPAAECLAIVLTDLGTSLKLAGNTQEGIQKYYEALKIDPHYAPAYYNLGVVYSEMMQYDNALSCYEKAALERPMYAEAYCNMGVIYKNRGDLEMAITCYERCLAVSPNFEIAKNNMAIALTDLGTKVKLEGDVTQGVAYYKKALYYNWHYADAMYNLGVAYGEMLKFDMAIVFYELAFHFNPHCAEACNNLGVLYKDRDNLDKAVECYQMALSIKPNFAQSLNNLGVVYTVQGKMDAAASMIEKAILANPTYAEAFNNLGVLYRDAGNITMAIDAYEECLKIDPDSRNAGQNRLLAMNYINEGLDDKLFEAHRDWGWRFTRLHPQYTSWDNLKDPERPITIGYISPDFFTHSVSYFIEAPLTHHDYTKYKVVVYSAVVKADAKTYRFRDKVLKKGGVWKDIYGIDEKKIASMVREDKIDILVELTGHTANNKLGTMACRPAPVQVTWIGYPNTTGLPTVDYRITDSLADPPDTKQKQVEELVRLPDCFLCYTPSPEAGPVCPTPALSNGFVTFGSFNNLAKITPKVLQVWARILCAVPNSRLVVKCKPFCCDSIRQRFLTTLEQLGLESKRVDLLPLILFNHDHMQAYSLMDISLDTFPYAGTTTTCESLYMGVPCVTMAGSVHAHNVGVSLLTKVGLGHLVAKNEDEYVQLSVDLASDVTALSKLRMSLRDLMAGSPVCNGPSFAVGLESAYRNMWKKYCKGEVPSLRRMEMLQKEVHDDPLISKDLGPSRVSVTGEATPSLKANGSAPVPSSLPTQSPQLSKRMDSTS