Protein: Proliferation marker protein Ki-67 (Antigen identified by monoclonal antibody Ki-67 homolog) (Antigen KI-67 homolog) (Antigen Ki67 homolog) (E9PVX6)
Organism: Mus musculus (Mouse)
Function: Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity). Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
Subcellular location: Chromosome {ECO:0000269|PubMed:12355204, ECO:0000269|PubMed:8834799}. Nucleus {ECO:0000269|PubMed:12355204, ECO:0000269|PubMed:8834799}. Nucleus, nucleolus {ECO:0000269|PubMed:12355204, ECO:0000269|PubMed:8834799}. Note=During early mitosis, relocalizes from nucleoli to the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:12355204, PubMed:8834799). Associates with satellite DNA in G1 phase (By similarity). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (By similarity). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (By similarity). {ECO:0000250|UniProtKB:P46013, ECO:0000269|PubMed:12355204, ECO:0000269|PubMed:8834799}.
Interaction partners:
- DNA topoisomerase 2-alpha
- Kinesin-like protein KIF15
- MKI67 FHA domain-interacting nucleolar phosphoprotein
- Cyclin-A2
- Centromere protein F
- Abnormal spindle-like microcephaly-associated protein homolog
- Mitotic checkpoint serine/threonine-protein kinase BUB1
- Centromere-associated protein E
- Forkhead box protein M1
- Cyclin-dependent kinase 1
Sequence:
MASSAHLVTIKRSGDDGAHFPLSLSSCLFGRSIECDIRIQLPVVSKRHCKIEVKEQEAILYNFSSTNPTQVNGVTIDEPVRLRHGDIITIIDRSFRYEDGNHEDGSKPTEFPGKSLGKEPSRRASRDSFCADPDGEGQDTKASKMTASRRSFVYAKGLSADSPASDGSKNSVSQDSSGHVEQHTGRNIVEPTSGDLFKKSRSTGSSYREPKSSPTQSLSNSNEKESPFEKLYQSMKEELDVKSQKSCRKSEPQPDRAAEESRETQLLVSGRARAKSSGSTPVTAASSPKVGKIWTERWRGGMVPVQTSTETAKMKTPVRHSQQLKDEDSRVTGRRHSVNLDEGESAQAVHKTVTPGKLATRNQAAVEAGDVASPADTPEHSSSKKRSIPAKVEAPSAETQKRLSLTQRLVPGEKKTPKGSFSKPEKLATAAEQTCSGLPGLSSVDISNFGDSINKSEGMPMKRRRVSFGGHLRPELFDENLPPNTPLKRGETPTKRKSLGTHSPAVLKTIIKERPQSPGKQESPGITPPRTNDQRRRSGRTSSGSKFLCETDIPKKAGRKSGNLPAKRASISRSQHGILQMICSKRRSGASEANLIVAKSWADVVKLGVKQTQTKVAKHVPQKQTSKRQRRPSTPKKPTSNLHNQFTTGHANSPCTIVVGRAQIEKVSVPARPYKMLNNLMLNRKVDFSEDLSGLTEMFKTPVKEKQQQMSDTGSVLSNSANLSERQLQVTNSGDIPEPITTEILGEKVLSSTRNAAKQQSDRYSASPTLRRRSIKHENTVQTPKNVHNITDLEKKTPVSETEPLKTASSVSKLRRSRELRHTLVETMNEKTEAVLAENTTARHLRGTFREQKVDQQVQDNENAPQRCKESGELSEGSEKTSARRSSARKQKPTKDLLGSQMVTQTADYAEELLSQGQGTIQNLEESMHMQNTSISEDQGITEKKVNIIVYATKEKHSPKTPGKKAQPLEGPAGLKEHFETPNPKDKPITEDRTRVLCKSPQVTTENITTNTKPQTSTSGKKVDMKEESSALTKRIHMPGESRHNPKILKLECEDIKALKQSENEMLTSTVNGSKRTLEKSKKKAQPLEDLTCFQELFISPVPTNIIKKIPSKSPHTQPVRTPASTKRLSKTGLSKVDVRQEPSTLGKRTKSPGRAPGTPAPVQEENDSTAFMETPKQKLDFAGNSSGSKRRSRTSKNRSQPLEDLDGFQELFQTPAGASDSVTVEESAKISLESSQAEPVKTPASTKRRSKMSLMKVDMKELSILEKQTQSRGRDAGTPAPMQEGNGTTAIMETPKQKLDFTGNSTGHKRRPRTPKIRAQPLEDLDGFQELFQTPAGANDSVTVEESAKMSLESSQAEPVKTPASTKRLSKTDLSKVDVREDPSILGKKTKSPGRAPGTPAPVQEENDCTAYMETPKQKLESIENLTGLRKQSRTPKDITGFQDSFQIPDHANGPLVVVKTKKMFFNSPQPESAITRKSRERQSRASISKIDVKEELLESEEHLQLGEGVDTFQVSTNKVIRSSRKPAKRKLDSTAGMPNSKRMRCSSKDNTPCLEDLNGFQELFQMPGYANDSLTTGISTMLARSPQLGPVRTQINKKSLPKIILRKMDVTEEISGLWKQSLGRVHTTQEQEDNAIKAIMEIPKETLQTAADGTRLTRQPQTPKEKVQPLEDHSVFQELFQTSRYCSDPLIGNKQTRMSLRSPQPGFVRTPRTSKRLAKTSVGNIAVREKISPVSLPQCATGEVVHIPIGPEDDTENKGVKESTPQTLDSSASRTVSKRQQGAHEERPQFSGDLFHPQELFQTPASGKDPVTVDETTKIALQSPQPGHIINPASMKRQSNMSLRKDMREFSILEKQTQSRGRDAGTPAPMQEENGTTAIMETPKQKLDFIGNSTGHKRRPRTPKNRAQPLEDLDGFQELFQTPAGASDPVSVEESAKISLASSQAEPVRTPASTKRRSKTGLSKVDVRQEPSTLGKRMKSLGRAPGTPAPVQEENDSTAFMETPKQKLDFTGNSSGHKRRPQTPKIRAQPLEDLDGFQELFQTPAGANDSVTVEESVKMSLESSQAEPVKTPASTKRLSKTGLSKVDVREDPSILEKKTKSPGTPAPVQEENDCTAFMETPKQKLDFTGNSSGHKRRPRTPKIRAQPLEDLDGFQELFQTPAGASDSVTVEESAKMSLESSQAKPVKTPASTKRLSKTGLSKVDVREDPSTLGKKTKSPGRAPGTPAPVQEENDSTAFMETPKQKLDFAENSSGSKRRSRTSKNRSQPLEDLDGFQELFQTPAGASNPVSVEESAKISLESSQAEPVRTRASTKRLSKTGLNKMDVREGHSPLSKSSCASQKVMQTLTLGEDHGRETKDGKVLLAQKLEPAIYVTRGKRQQRSCKKRSQSPEDLSGVQEVFQTSGHNKDSVTVDNLAKLPSSSPPLEPTDTSVTSRRQARTGLRKVHVKNELSGGIMHPQISGEIVDLPREPEGEGKVIKTRKQSVKRKLDTEVNVPRSKRQRITRAEKTLEDLPGFQELCQAPSLVMDSVIVEKTPKMPDKSPEPVDTTSETQARRRLRRLVVTEEPIPQRKTTRVVRQTRNTQKEPISDNQGMEEFKESSVQKQDPSVSLTGRRNQPRTVKEKTQPLEELTSFQEETAKRISSKSPQPEEKETLAGLKRQLRIQLINDGVKEEPTAQRKQPSRETRNTLKEPVGDSINVEEVKKSTKQKIDPVASVPVSKRPRRVPKEKAQALELAGLKGPIQTLGHTDESASDKGPTQMPCNSLQPEQVDSFQSSPRRPRTRRGKVEADEEPSAVRKTVSTSRQTMRSRKVPEIGNNGTQVSKASIKQTLDTVAKVTGSRRQLRTHKDGVQPLEVLGDSKEITQISDHSEKLAHDTSILKSTQQQKPDSVKPLRTCRRVLRASKEDPKEVLVDTRDHATLQSKSNPLLSPKRKSARDGSIVRTRALRSLAPKQEATDEKPVPEKKRAASSKRHVSPEPVKMKHLKIVSNKLESVEEQVSTVMKTEEMEAKRENPVTPDQNSRYRKKTNVKQPRPKFDASAENVGIKKNEKTMKTASQETELQNPDDGAKKSTSRGQVSGKRTCLRSRGTTEMPQPCEAEEKTSKPAAEILIKPQEEKGVSGESDVRCLRSRKTRVALDSEPKPRVTRGTKKDAKTLKEDEDIVCTKKLRTRS