Protein: Ankyrin repeat and LEM domain-containing protein 1 homolog (EC 3.1.-.-) (LEM-domain containing protein 3) (G5EGA3)
Organism: Caenorhabditis elegans
Function: Endonuclease which, in association with baf-1, plays an essential role during embryogenesis in the DNA repair response following DNA damage probably by ensuring proper chromosome segregation. Also required during postembryonic cell divisions after DNA damage caused by ionizing radiation to ensure normal cell proliferation. Resolves chromatin bridges in late mitosis that result from incomplete DNA replication, defective chromosome condensation or unresolved recombination intermediates. Together with brc-1, contributes to genome integrity by resolving mitotic chromatin bridges that result from incomplete processing of DNA breaks. In parallel to the slx-1/mus-81 pathway, acts in processing early recombination intermediates in meiotic prophase I to prevent illegitimate recombination. Also involved in processing remaining, erroneous recombination intermediates that persist into the second meiotic division.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:22383942, ECO:0000269|PubMed:29463814, ECO:0000269|PubMed:29879106}. Nucleus {ECO:0000269|PubMed:29879106}. Chromosome {ECO:0000269|PubMed:29463814}. Midbody {ECO:0000269|PubMed:29463814}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:29463814}. Note=Localizes to foci-like structures outside the nucleus (PubMed:22383942, PubMed:29463814, PubMed:29879106). In pachytene, localizes to foci both in and outside of the nucleus (PubMed:29879106). Colocalizes with air-2 between dividing nuclei in meiosis II (PubMed:29879106). In mitosis, localizes to the center of chromatin bridges that are formed in response to DNA defects (PubMed:29463814). Forms initially a ring like structure encircling chromatin bridges between two separated daughter cells until in late telophase, localizes to the center of chromatin bridges (PubMed:29463814). Colocalizes with air-2 at the midbody (PubMed:29463814). Midbody localization depends on the formation of the central spindle and the midbody and on air-2 (PubMed:29463814). Colocalizes with the central spindle component zen-4 at the central spindle (PubMed:29463814). {ECO:0000269|PubMed:22383942, ECO:0000269|PubMed:29463814, ECO:0000269|PubMed:29879106}.
Interaction partners:
- Cell cycle checkpoint protein RAD1 homolog mrt-2
- Checkpoint protein
- Structure-specific endonuclease subunit SLX1 homolog
- Ankyrin repeat and LEM domain-containing protein 2 homolog
- DNA repair endonuclease XPF
- Cell cycle checkpoint protein hpr-9
- Emerin homolog 1
- Crossover junction endonuclease MUS81
- LEM protein 2
- GEN1 Holliday junction resolvase homolog
Sequence:
MPPNGAITTTPRSRMPPTTPSSGKSRPKKETLHYLAASSSTTSVDAARTLLERGANVNAIDRDGATPLHYACTHDNVAMAQLLLTFGADPMSADKLGRTAYSIAKGNTLRFLRRYKKSSNRQRLGFFRRFFACHSRNETFFIVRNNQEVAPLRPTALAEAASISFNRGNVLTNSYRCAKKKIRATFHAIRRSRSNSTATLQDVVLTSEGIRTVTTPSRRAPKATVYAKRSMSVSDLLLIPDRRDIKNEDVKTRGSPVKKTRGTGRSRTPEAILNPRKQRTPVNHHKRSKSQETKLVAMPSPNSMAYYNTSRARNAGLRPAPSAPPLSPEPAIAAVKTPKTPTTPKTSKGRSKSPANTTAYFTADESLELLGNNMEKLNIESKSAKSSKLKTPSKPTTSSSTSFSSAEDDKEAEVSTPTTVDDGEIRKIRRLREGELKSELKKFGISPAGPLDARTRRLYEKKLLIERRKITNRGYSPDADVVSCRNSPQLELVLRNGFLPADFASRARKCDENVRSEFSGNGFGYNAFCYLIMDPRILGSNVENLTLETFVRSIFYVGKGSKNRPLAHFIDARNERRDKLDKLKTCEKLKTIDELWTLGFGIPRHEISHGVSDEEAFVREASIIEAVKLKNLRNKKAGEFHGTTKSWDNITKSEYGTFLLDRALSTLKLEGIRLITEDNLPDSLYPYVNNRRGAGGGRTPKTPK