Protein: Transmembrane channel-like protein (A0A0U1QT59)
Organism: Drosophila melanogaster (Fruit fly)
Function: Probable ion channel. Component of mechanosensitive neurons that participates in proprioception, sensing food texture, and directing egg-laying site selection (oviposition). Component of multi-dendritic neurons of the labellum (md-L) where it is required for sensing the hardness and viscosity of their food, enabling them to behaviorally discriminate their preferred softness and smoothness from harder and stickier food options. Required as part of oviposition site selection process to relay mechanosensory and chemosensory information on the hardness and sweetness of potential egg-laying substrates, thus ensuring females select the most optimal site for their eggs survival. Females determine the softest substrate for their eggs first by making a coarse evaluation of substrate hardness using mechanosensitive channels nan and Piezo in the leg tarsal bristles, followed by a much finer assessment using nan, iav and Tmc mechanosensitive channels on the labellum. This protein is required to sense subtle differences in substrate stiffness (between 0.25% and 0.3% agarose), likely acting in the md-L neurons. Also required in neurons on the labellum, including the md-Ls, and possibly in the brain, to inhibit discrimination of egg-laying substrates of different hardness if the substrate contains sucrose. During oviposition evaluation, activation of sweet neurons by sucrose enhances the activity of the Tmc neurons resulting in females losing their softness preference in favor of egg-laying sites that contain sucrose. Acts in the larvae peripheral sensory neurons, to contribute to proprioception and sensory feedback for normal forward crawling behavior. Required for the normal activity of the proprioceptive sensory dendrites, ddaE which show preferential responses to forward locomotion, and ddaD which show preferential responses to backward locomotion.
Subcellular location: Cell membrane {ECO:0000255}; Multi-pass membrane protein {ECO:0000255}. Cell projection, dendrite {ECO:0000269|PubMed:27478019}.
Interaction partners:
- GH28601p
- Uncharacterized protein, isoform C
- VWFC domain-containing protein
Sequence:
MQNDEEPAAAAGTSGLSNGESLRSPPAPAPRRPKPGILRLDIGKPRRSSGGSVDFRCVGSSSSNGNTSNVATGANSENNSGVTSPHQLSVTWAPPCDLDRGGWQMQSSADAKREFYKGQRGRRAASQEDHRSYELNDFPLQNQSSDAESCHQEPHFAHQRSPGIGFDEDGGGGDIDDEESYTISVSAIMQRRASVRGYRGKRGSRSSRRASSPMDHVLDSVERRRSSVYTTSSEEGTNQESTQEQIFENIRLHKEVIQSVKLQPWPIRKKLKLVRQAKTYVARHEGALQERFAMSRSTRDLWARFKILMAARWRHWKRETASFLTVLIPWELRIKEIESHFGSGVASYFTFLRWLMWVNIMIAIPLVAFVIGPEYFATKHGETDPRKRMSDPEARVAGNLFTFWEFEGYLKYSPMFYGYYSSTSGISTSGYKLPLAYFLTAVLVYIYSFVATLRKMAENSRNSKLSSKDDECVFSWKLFTGWDFMIGHAETAHNRIASVVVGFKEALLEEAEKKKDNRNWRVILQRILVNILVMGLLGLSGATVVLLVNHSEDLAKHDNWLSRNAVNVTMTLLSFFLPMIFEALGLFENWHPRQQLRLQLARIMILNMLNLYSLMFSFIYKINSKEKPLQMLKLENETNTMELKNLLSSIEALRAMTPTTSLYGESTSDGLFDDSTSTATWGEDGGGLFSTTAAAALISTTVQRLKCYNMTVKCSKLRRNIISGKHLATTLMVLNLTTPAMVPPTLPTTLPTTFPTTLPTTLPTTLPTALPTTLPTTLPTTLPSTLATTTATTSSIWSTTEETSPTTTTTSPWTTLPPSTTTTEATTTTERATTTTEATSTTTLKITTAEINSTLSDTTKPLGKSIDTEIPNSTTNSATLSTIPATLNTTNLPLNSTTKLTTTTSTEKPQGEDNFIYTTGEDEGSYDYGSDSTSDAPDNNSYSDITDYSSEPSEIEDFDEQESTDQADDPLAKVLEQLDENETKGRRKRALAESPFFTSKYSRRHRNESAVSAGQPRETTESVNATPSRWPFNWASFRQTTPRTTTTRRVPSGILTKEEWERLRRLRGRITTTTSTSTTSTTTRRPRWRYRTTTTELTSTTEEESSTTESSTDSSSPGSTTNAFDSSSSTTEEDEYTTTEGSENRPYYVGYVDISEMGSTIYYDGDSEFLEECVITICPKGDDFFGSTTESPDSTTQSSDSKQLTTVKLTPLERKQKRLKEVQLAIKQIQTNLTTMCWETSLGQELSKVIVFDGLMSIVAPLCIDFLRALFVRYVNQNWCWDMEKTFPQYGDFKIAENILTLINNQGQVWMGIFFSPGLVLINLVKLMIMMYFRSWIVLTCNVPHEVVFKASKSNNFYLSLLLTMLFLCVLPVGYAIVWLRPSWHCGPFSEYNRIAEFITNTTRNALPKQLHEPLDYLTSSSTVIPLLLLLILIIYYLVSLTGALREANQDLRTQLQKEREEERKKIFKVPEVKQAEPTATTLTNRWRKVLEASSPVTPTQPPDFDTEEYKNQARKELISRIMKKALRKGSATSDEDSFVRRDDDDTDTEHQDSLPHDEEAKDKRFGLSRLQQIRRTRKPSLVDIVQIAKQERARAGSIVAGTSSSGTGNFPIKETHPKSRFKVEKHERKDRGSMKDKKDTRHRQSPQQQQQPPPYESPKDNEHDPDTNSRIVSASLLRRHKEQAEGEEPPTTPDAPQTPNSPVEPVEQALEESTPETPTLAKSKFHIVDEKKPPPHEVEDKPLPTPKESGSGGGSLGKFKFRKHKFKSNNVAAVKPEPEVFKFDERSVERSSDVPATHAAEYLNNEPSGTEEQDRSLPSPTPSQGQGHHQRQLSVLSRQGRKKIGNLLALVREAVNLKKDDVEQAGSDESPGPTTPTYLAYTPPPPPSVLSSVSSSTALEMPPTPEPESPTPSAPLHFGSSTSSRAPSKPPKPPMVPASATAPTATMDDLEELDTAGPITFPRRSDSHRRRTMRQDSQSSIWSDNIPTITISTTGSDECIVDAAAPQNGLPDPRSASPEPTVNIIRIDIENEHEK