Protein: Callose synthase 12 (EC 2.4.1.34) (1,3-beta-glucan synthase) (Protein GLUCAN SYNTHASE-LIKE 5) (Protein POWDERY MILDEW RESISTANT 4) (Q9ZT82)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Involved in sporophytic and gametophytic development. Required for normal leaf development. During pollen formation, required for the formation of the callose wall separating the tetraspores of the tetrad (interstitial wall), but not for the callose wall surrounding the pollen mother cells (peripheral wall). Functionally redudant to CALS11 (GSL1). May play a role later in pollen grain maturation. Required for callose formation induced by wounding and pathogen attack. May interfere with salicylic acid-induced signaling pathway during defense response. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals.
Subcellular location: Cell membrane {ECO:0000269|PubMed:12081364}; Multi-pass membrane protein {ECO:0000269|PubMed:12081364}.
Interaction partners:
- Ras-related protein RABA4c
- Probable pectate lyase 13
- Protein PMR5
- Glucan endo-1,3-beta-glucosidase 10
- Arabidiol synthase
- Plasmodesmata-located protein 5
- Protein DOWNY MILDEW RESISTANCE 6
- Isochorismate synthase 1, chloroplastic
- MLO-like protein 2
- Pathogenesis-related protein 1
Sequence:
MSLRHRTVPPQTGRPLAAEAVGIEEEPYNIIPVNNLLADHPSLRFPEVRAAAAALKTVGDLRRPPYVQWRSHYDLLDWLALFFGFQKDNVRNQREHMVLHLANAQMRLSPPPDNIDSLDSAVVRRFRRKLLANYSSWCSYLGKKSNIWISDRNPDSRRELLYVGLYLLIWGEAANLRFMPECICYIFHNMASELNKILEDCLDENTGQPYLPSLSGENAFLTGVVKPIYDTIQAEIDESKNGTVAHCKWRNYDDINEYFWTDRCFSKLKWPLDLGSNFFKSRGKSVGKTGFVERRTFFYLYRSFDRLWVMLALFLQAAIIVAWEEKPDTSSVTRQLWNALKARDVQVRLLTVFLTWSGMRLLQAVLDAASQYPLVSRETKRHFFRMLMKVIAAAVWIVAFTVLYTNIWKQKRQDRQWSNAATTKIYQFLYAVGAFLVPEILALALFIIPWMRNFLEETNWKIFFALTWWFQGKSFVGRGLREGLVDNIKYSTFWIFVLATKFTFSYFLQVKPMIKPSKLLWNLKDVDYEWHQFYGDSNRFSVALLWLPVVLIYLMDIQIWYAIYSSIVGAVVGLFDHLGEIRDMGQLRLRFQFFASAIQFNLMPEEQLLNARGFGNKFKDGIHRLKLRYGFGRPFKKLESNQVEANKFALIWNEIILAFREEDIVSDREVELLELPKNSWDVTVIRWPCFLLCNELLLALSQARELIDAPDKWLWHKICKNEYRRCAVVEAYDSIKHLLLSIIKVDTEEHSIITVFFQIINQSIQSEQFTKTFRVDLLPKIYETLQKLVGLVNDEETDSGRVVNVLQSLYEIATRQFFIEKKTTEQLSNEGLTPRDPASKLLFQNAIRLPDASNEDFYRQVRRLHTILTSRDSMHSVPVNLEARRRIAFFSNSLFMNMPHAPQVEKMMAFSVLTPYYSEEVVYSKEQLRNETEDGISTLYYLQTIYADEWKNFKERMHREGIKTDSELWTTKLRDLRLWASYRGQTLARTVRGMMYYYRALKMLAFLDSASEMDIREGAQELGSVRNLQGELGGQSDGFVSENDRSSLSRASSSVSTLYKGHEYGTALMKFTYVVACQIYGSQKAKKEPQAEEILYLMKQNEALRIAYVDEVPAGRGETDYYSVLVKYDHQLEKEVEIFRVKLPGPVKLGEGKPENQNHAMIFTRGDAVQTIDMNQDSYFEEALKMRNLLQEYNHYHGIRKPTILGVREHIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKVRMHYGHPDVFDRFWFLSRGGISKASRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQISMFEAKVASGNGEQVLSRDVYRLGHRLDFFRMLSFFYTTVGFFFNTMMVILTVYAFLWGRVYLALSGVEKSALADSTDTNAALGVILNQQFIIQLGLFTALPMIVEWSLEEGFLLAIWNFIRMQIQLSAVFYTFSMGTRAHYFGRTILHGGAKYRATGRGFVVEHKGFTENYRLYARSHFVKAIELGLILIVYASHSPIAKDSLIYIAMTITSWFLVISWIMAPFVFNPSGFDWLKTVYDFEDFMNWIWYQGRISTKSEQSWEKWWYEEQDHLRNTGKAGLFVEIILVLRFFFFQYGIVYQLKIANGSTSLFVYLFSWIYIFAIFVLFLVIQYARDKYSAKAHIRYRLVQFLLIVLAILVIVALLEFTHFSFIDIFTSLLAFIPTGWGILLIAQTQRKWLKNYTIFWNAVVSVARMYDILFGILIMVPVAFLSWMPGFQSMQTRILFNEAFSRGLRIMQIVTGKKSKGDV