Protein: Titin (D-Titin) (Kettin) (Q9I7U4)
Organism: Drosophila melanogaster (Fruit fly)
Function: Key component in the assembly and functioning of adult and embryonic striated muscles and muscle tendons. By providing connections at the level of individual microfilaments, it contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensibility properties of muscle. In non-muscle cells, seems to play a role in chromosome condensation and chromosome segregation during mitosis. Might link the lamina network to chromatin or nuclear actin, or both during interphase.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:11062264}. Nucleus {ECO:0000269|PubMed:9548712}. Chromosome {ECO:0000269|PubMed:9548712}. Note=Uniformly distributed along condensed mitotic chromosomes (PubMed:9548712).; SUBCELLULAR LOCATION: [Isoform A]: Cytoplasm, myofibril, sarcomere, Z line {ECO:0000269|PubMed:22467859, ECO:0000269|PubMed:26251439, ECO:0000269|PubMed:8335002}. Note=Colocalizes with ball and mask in Z-lines of indirect flight muscle (PubMed:26251439). In the embryo, localizes across the muscles in a striped pattern positioned at the sites at which muscles are attached to the epidermis. In the larval body wall muscles, localizes to the Z line and is still present as a doublet at the muscle-epidermal attachment sites. During pupa development, progressively forms broad striations at the Z line which become more defined after the pupa eclosion. {ECO:0000269|PubMed:26251439}.
Interaction partners:
- Myosin heavy chain, muscle
- PDZ and LIM domain protein Zasp
- Actin, indirect flight muscle
- Bent, isoform F
- Alpha-actinin, sarcomeric
- Muscle-specific protein 300 kDa, isoform G
- Rolling pebbles, isoform B
- Muscle LIM protein Mlp84B
- Lethal
- Troponin T, skeletal muscle
Sequence:
MQRQNPNPYQQQNQQHQQVQQFSSQEYSHSSQEQHQEQRISRTEQHVQRSQVTTQRQVQQHHGGSIGGAYVPPSLTHVYAQGDISPPVFEQIFKNARFAQGGNALFEGRLRGNPKPFVTWTRKGAPLLESQKFRMSYNEATGDVSLLINQIGPGDEGEYTCTARNQYGEAICSVYIQPEGAPMPALQPIQNLEKNIYSNGYSYTSIEEEFRVDTFEYRLLREVSFREAITRRSGYEQDSQLSQELDRNQGPAQAPQISQKPRSSKLIEGSDAVFTARVGSNPKPRLTWFHNGQRLVASQKYEISYSSGVATLRVKNATARDGGHYTLLAENLQGCVVSSAVLAVEPAAETAYEPKPVDVMAEQLEAGKALPPAFVKAFGDREITEGRMTRFDCRVTGNPYPEVFWLINGRQVRDDASHKILVNESGSHSLMITNVTRLDAGAVQCLARNKAGEVAIEAQLNVLEKEQVVAPQFVQRFSTMTVREGEPITMSANAIGTPQPRITWQKDGVQISSTAERFVGIDGGATCLEIPRVTANDAGWYQCTAQNIAGSTANRARLYVEVPREQPNYEQRRLNLPRPTKVIEPEPIPGPEIIYLRHVERAKPHLRPGEEDRVYPPPQFIIPLQNVQQTEGGRVHMEARIEPVGDPTMVVEWYLNGRPLAASARATSVFKFGFIALDLLSIMGHDSGEYMCRVTNASGVAESRAILSVVQRPSIEQSSQNPNSLQYINQLEDYSRYQRTESIDEQLNQAPQFIRPLRDLGEFEEGKNVHFEAQVTPVNDPSMRVEWYKDGLPITASSRITAIFNFGYVSLNILHLRAEDAGTYTVRAVNRIGEAISQSSIRVHSRSQVTADLGIPEQQRYIEKVEELEDYRKSQQRRHVQEAAEAIAPPQFKTPIQNQLDLREHAHAHFEARLEPVGDSTMRVEWLKDGQPLEASSRITTYHNFGYVALTIKQLTIYDAGTYTCRAYNAMGQDTTVAQLTVISKNEIVSESQHPGGLQKIQHLEDSSRYGRREEEETYITQAPRFLGPLKGTTKILEGQRAHFEARVEPQSDLGLVIEWYHNGRSITAANRIQTYYDFGYVALDISQVRAEDAGVYLVVARNKLGEAQQQATMIVETRSSIDTSSMHRGLYEKTQNLENKPFVEPQYDIEEISKSKPVFVTPLSDPKPIHDGKNIHLECRLEPMGDPTMRVEWFHNGRPVTVGSRFRTYYDFGFVALDIIKATAADSGEYTVRATNHLGTAHTSACVRVIDHTDVVTETQNEQSLEQIQLLEDSRRRHHQEEDITIMQAPQFTRGLHNIETIEGTNVHLECRLQPVGDPSMRIEWFVNGKPVKTGHRFRPAYEFDYVALDLLGCYAIDSGVYTCQARNQLGEAVTSCSVRIIAKNDLILETQNESGLQKIQYLEDSTRHRRSEFVDEVVNIRPRFLTHPKSLTNTREGGHAHFECKIEPVTDPNLKVEWFKNGRPITVGHRFRPIHDFGYVALDIVHLIAEDSGVYTCRAVNLIGSDETQVELQCRSGEQIVTVTQNEAGLEQIHYLEDRSRYTRREEIDESTKQAPVFTTSLKNVEIKENQRAHFECRLIPVSDPSMRVEWYHNNLPLKSGSRFTETNNFGFVALDIMSTLPEDAGTYTCRAYNAVGEAITSAVAVVHTKKSIYLESQHETALPRLQHLEDGSKRQRISVQDEFVSQAPVFTMPVRDVRVAENQAVHFEARLIPVGDPKLTVEWLRNGQPIEASNRTTTMHDFGYVALNMKYVNPEDSGTYTCRAVNELGQAVTSASLIVQSKTSIQLETQHEAAMHKIHQLEDHSRYQRREEEEYTVTTAPVFVTKLIGPSNLVEGQSAHYECRIEPYPDPNLKVEWFHNGKPLSTGHRFRTTYDFGFAALDILTVYAEDSGEYTCRVTNNLGEAINSIVLNVTSRSSIIHETQHEEALTKIQHLEDTSRFQRKTDEEQFHAERPQFGRPLRNAKVNEGAPVHLEATLIPVNDPTMKVEWYCNGRPIQTGHRFKTTYDFGFVALDILYAHAEDTGTYMCKAKNAIGEAVTTCAVNVTANKTLDLDTLDAQRLEKIRQLETYAPPPKPVVEEKGQKPIFLTPLSNLEHLKEGEHAHLECRVEPINDPNLKIEWFCNGKQLPTGHRYRTTHDFGYVALDILYVYGEDTGTYICKATNQLGEAVNTCNVRVLNRRSMILDTQHPDALEKIQKLESKVPNARTEVGDAPISPPHFTAELRGSTEIYEGQTAHFEAQVAPVHDPNLRIEFYHNGKPLPSASRFHITFDFGYVSLDITHAVAEDAGEYSVRAVNALGQAVSSTNLRVIPRGTIISDTQHPEGLEKIRKLESTAPHQRQEPETPGTRQRPVFTQPLQNIDRINEHQTAHFEARLIPVGDPNLKVEWYRNEKIIEDSSRITKQHDFGFVSLDISHIRKEDEGVYMCRAVNPLGEAVTTASMRVVSEASIQMDTQHPDSISRIHQLEKPLAPRPTEPERLFEKPIFTQLLTGPSELWEGTHAHFEARVVPVGDPSLKFEWFINGVELQMGSRLRTTHDFGFVTLDITAVVPEDAGVYMCRAYNAAGEAVSSTAMKVKTKSNIDGQPLIPESWEAIRLKEAAMNRVPEMFVDSTPQQAPVFTTHLQSYDKLHEGQHVLLEAQVEPRADPNLRIEWFKNGISLTTGSRIRSTFDFGLVTLSINGLRADDSAIYTCKATNQVGEAVSTSSLKIEDRHWLQAESLHPDSLPRIGELEAPKEGRPEAPEPTYETPVFITHLNNIECKESDNVRFECNVEPARDPTMSIEWFYNGQPLQAAAKFKSIYDFGYCALDLTNSYAENSGVYTCKATNSKGSATTSGTLKCTGGKTMFLDTQHPQGEAGLEAVQETEEELANRYTSKTTKPETQYPPPVWTKPLQAEFHLSEAQPIHLEANVEPKEDPNLFIEWYFNGKMLNHGSRFKMTSEFGFVTMDMIEVYARDQGIYTCKAYNKAGEAFTSTTIFCSSKENIIESTQHPKGAEGLEQIQDLEDSLRKDGSKPEQPDLGIPPRFTTEFVNIADIGEGELAHFEANLIPVGDQSMVIEWFYNGKVLEASHRVRTIYAFGTVALEVLGTKIEDTGTYTCRATNKHGTAEISCNLECVDKPRGQKPRFTSHIQPLEGLKDGQSAHFECTLIPVNDPDLKVEWYHNGKLMRHSNRIKTVSDFGYVVLDISYLQDHDSGEYVCRAWNKYGEDFTRTTLNCGGRGGVFYDSLQPDSLQRIRELECPQGQQADTSAPLVAEPPKFITQIVDVTKLVEGQSAHFEARLTPITDPDLVVEWYFNGKKLPHGHRFRTFHDFGIVILDILYCYEENSGVYEARARNKYGEDVTRASLKCASKSSLILDSQLPRGMEGGLEKIANLEYSMVRTREETTEETKGKAPVFTVPLENIENLREGENAHFEARITPADDPKLKVEWYWNGRPLKAGSRFRTFCDFGFVILEISPVYPEDSGEYSCRAINEYGEAVTTATMKIQGKRSIIMESQLPKGMEGTIDRIAELEGLGSRSTEFVPDDDTGKPPEFITSPFDMVIGENALAHFECRLQPINDPSMRVDWFHNGKALWAGSRIKTINDFGFVILEIAGCYQRDSGLYTCKATNKHGEATVSCKLQVKGRQGIVMEPQLPSNFRTGTESLQKLEETMHKREELVTEDEQPNPPKFTEEIKDNLDVPEGGPIHFDCRVEPVGDPTMRIEWFYNGHVMATGSRVHQLNDFGFIALDVDYIYARDSGEYTCRATNKWGTATTSAKVTCKGKHNIVYESQLPEGMTSEKLKELERGRIPEAPKVVEEVFGPPKFTTQITSVTVDEAEAVRFECQVEPKTDPSLRVEWYRNGKPLPSGHRYRNIFDMGFVSLDILYVYGEDSGEYVCRAINNYGEDRTRATVSCKKLPTILLQNQVPRGMKRSDALTQMEATIKKYTSEVHLTEDDLFDPDRKQPPRFVTQIKEQLTLTEMAVTKFECQLAPVGDPNMKVEWFFNGKPLLHKNRFQPIYDFGYVAMNFGWVYPEDSGEYVCRATNLYGKDETRAIIKVSGKPGIVYDSQLPAHMQSIDRIREMEASWQVVPDEVDPDAKPRTKPVFVSKLEPQTVEEGDPARFCVRVTGHPRPRVMWLINGHTVVHGSRYKLTNDGMFHLDVPKTRQYDTGKVEVIARNSVGESIATTELKVVARSDDYRNVLKNSPRPWYDYELAAYQKERQENELEKVFDERKQVLSEQSSHTLKGVEHLKPKQYKPPTPDWQQNVKAKKSEDYYNKLQTLETEQLLKETNLRRDTHQYAIPGEKVVSSSQAKGMAQSYEENLQEKTSTTEVQAAPPKGIAQPSESSVHGREVHMNKQQQVQKEIQGDLEITRKITATETTEVEHKGTIQERVVQGPVKPAKAPVFTKKIQPCRVFENEQAKFEVEFEGEPNPTVKWYRESFPIQNSPDLQIHTFSGKSILIIRQVFVEDSAVFSCVAENRGGTAKCSANLVVEERRRAGKGGIQPPSFVTTIQSTTVATGQLARFDAKVTGTRPLDVYWLKNGMKIQPSIKFKMLEEDSVHTLLIIEPFAEDSGRYECVAVNAAGEARCDGDCIVQSPSKPEKPTTPGSEKAPHIVEQLKSQTVEEGSKVIFRCRVDGKPTPTARWMRGENFVKPSRYFQMSRQGEYYQLVISEAFPEDEGTYKCVAENKLGSIQTSAQLKVRPIENLDAPPTITALKDVSVTEGMPAQFKTTVTGKVKATSVQWFREGQLIPETPDFQMIFDGNSAVLLIGTTYEEDSGIFTVRVTSSTGQVESSAKLTVKKRRISAFQLRTIDSAEDESSSSGREDSAPESPHAFQPGQQPGQQFGQFLGVNGQGQHQGRSRQKKPKVRSKSLQPATKVIPWRKSSRPTRGRSLDKGVFLPGFKPEPVKSWTEETINLKATPIEKKKPAPKLEAAKVVLKSIKTERDQGIMSLGATLEQIIAGKTEKEAIPWITMREKLKAVESVQQQLNKFDLDEVYLQPLEGQIETEGQLPQQAQVEQVQRTKEIQRLKSMESVEIMEMTDQIDKLITQQQNAKDLIPWKEMRQQLKSVQRVTKQIDKFKIEEVELRHLQAQQAITEEYQTGTAEETVVMIDESSKGSISKVLRRDEQLQYEDQSNIYKQKFITTEDVNIMHVSEREKLEAQRLIREQQAVNWRQQQQRPQLQPLTSVEDTVISQTSERQKLVQQQSFIEEAQRQQFVQVEDSQMMSLEEYEHQKIINQRTQQEAFSWRQPREPQKFIQVEDSTLLHLQERHDTQEQQLLQQQPVMWDRGRKKPDQPQYVQPQEQRVKEEFVEKPKTYEEMHDELVEPTPIEQPQPVPVMWERGKKKPQPQEKTFEEAHDELVEPTPVQQPEPVPVMWERGKKKVAQQETVLSQEVVQTSQVVEQQIVEETKKTAVRRVIPPREPEQKVEQVTLKPTPRPRPKEAVKAEEIQLKPLRSTRPVPQPVEAEQKAYEEATDELTEEPIPQPQPVMWERGKKKPQKPQEEVTEIPKTLEIAVDTLEEEVPKPTEPQPQPVLWARGQKKPQKPDEQKQELPKSLEIAVDTIEEDLIKPVQPEPQPVLWERKKKKPQPQDVIEEKLDVAPTKTYEKAVDVLPDEPKVEEKPEPVLWQRGKKKIPKSEPTEEVHPDEVDAQIETVVKEDEMIVEEKRRIKKTKRPKSTKEVTEELFEEQPEEEISPEEEVPQKEVIEEIEEIVEEKRRLKKTKKPKLTQQVTEEETPHEEIIKESEEVVQEQEEIVEEKKKVKKVKKPKTVAEKQLKEEEIPTEETVEEEETAEDQQLVVEESKKVKKVKKPTGTVEKTDVEELPGEEVPVEEVPVEEVPEDVAPEEELIEEQEEIVDQDEIQEQKRKVKKAKKPKKTIEKTEIEIEEDQPEEEVLQEEIIGEQEEITERQRKVKSIKKPKKVVTEKTVDQTEQPEKPEESQAEEVKETVTEEPKKPKPAPEEAKVEQVEKISLKPAPRKQRLLPEKEQVEEVLLKPVKKIVAVSEAEQPETPETEFEVKEFAITTTEDILDVTKKRVKKKKPKTKVAAEESTEEPAEETEEFEEEATQPEEVQPVEEIPEEPQVKEVADERKTAPKPKPRKEEIIEKVEEVALKRVTRPKKELPQEATIEEVRLKPTQRTSIKPEEVKLEEVDLQHVEKKEDEIVQEEKRKTRKVKKPKHEDLPEIPDAEPTQLEEAEHIELEKQPKPEEDQPQVPWKRGEKKQPVEEVLEEKKWPSGKRRPLPEQQPEEVQLKPIPSKPIEEQQKPEKAIPGPQLVPEEKPESEEEELELEPLKLPEDKKPKEPKAKKEKKKKPKLKKATPSVDEVSEEVAEPFDEPIAEEDEVEEMPVDDVKVVAVSEDVLPEEEVVPTEETPEAKQKAHKKRTKRLKEASVEGQPQLLEAAIAEIEKVDEISQEISQKTITLLKKTEDTRPQFITTEQLIELDVEDVRRDLEMKVTSNIIKKEKRRVVLDDSQPLPELELITQKRIQEGIDKVADEELIEDQQLIQNQQETTTSEVIGQERKLVKKKKKEIKPPRITEKLRPRQCVPEEPTVLECKVEGVPFPEIKWYFNDILLFASEKYEITVMEQVAKLKIAKVTPSDVGVYTCEAKNEAGVATSRTNIILEKEQGVPPQFTKPLKIEFIEEKQPERLKVTVTCQVTGKPNPEVKWYRGIEEVIPSETVQMFYDEKTGDVALEVINPTPNEAVVYSVQAQNQFGRAIGNANILSRVDEVPREILKAPTVTPLSAVVVPTGGTLFFEAKYDGLPRPEVKWMRNGREIIENEETIIETTETTTTIKVVNMTRKRTGKYEVWAKNKVGEAKSSGSVVVSDQKPDEQIKPPRFIQPLEPKYFGEHEVAIIEAIVESEPLSSFQWFVHNEPIKSSNEVRIVSQANKSTLLIENFQSKFVGPFTCRAENVGGSVTSTATVNLIPQEEAEEFESPRFVEELVQPVEVMDGEALLLTCQVTGKPTPKVEWYHNAEKITENKETTISQDLQGVCQLQITEVFPENEGQYECVATNKIGKSVSKTNVKIQAFEYIPDSEITGLTGSEEDLLDRTLSIDEQAPKIIKKLPEKIEPKEGEQAKLEVKVVGKPKPKVKWLRDDEQIFASEEYQIENFEDGTSVLVINHVYPDDLGTISFEAYNPLGVAVTTALFAVEGIVGSKDYRKPEWVSQMEEMQVALKAAKCSPSLLNEMRDCRAALGETAKFSIQFAGNPIPDIQWYFNNVQLRASEKYRMVVQEQEATLEIMKITSEDCGYYNCKLINEIGMTMTRAKFDISSTSTIVEETKAKTTVKKKSGKKTMVKRSGASESQNVQKTEIRIIPTSAVETSMNVIKVKQPVSVLVEKSEISEVLVVKDREVADAEERSSQLIEEIEEEEEIEEKVQHDEEDEVEVQVEQKETYTSSKKIEITKTVELIRTKISEKIITIEDVQVLSHHEEVQWLLESIEAESFGQIGESALRDLATIGLLLRYGCEHYEITYMYEQNIFISLKKPESQSALVQLVEREGHEELISQILSESSNEDETILAATVGFKAFIRMIQTYEITIEIVIRKFVREDFISQDWKICGKERIVETSQIIESHEAITHVKIETATTKVEKLFKKQEQEHVQNLEQQEQVKIQVQTKQIAQMNTKIKKHKKHKQQEQEVSETTIQCEQKETLAHETSAELPQSETLEQIEESLSTYETLPIQNLSKDTLQTVAVSVTTELSTPSPTASRVQEEILPQKVLAINEEVLPLDEFGLRKESPRPKENKLTENIEVRLKHALNVSHAKTAESSKELPSKIPKSVKAQRKMKESRSLVVEAPNAEEAIEDLKPLKAVSQEVQSDILFSHEITEEQHQALETIEKLKPTSAIEDTVQQKLLSQEELIIAEVLPSETVGRDVTDVRPPGETISPRLTPNMSLCITECQPEDSIGEMQQAAKERMETPSMSVTESKAVGGQELEVLENVDHMPLITQPTKGLADYTIKAEEVPVQVQEIITFDSLERETVAKTQTAKSNALELFELSEGLVSSTADSHSPIAEDLPIFEKDVKEATIDMQMQHHVTTSETVSNENAVKDLKAVDTPKMAEGTLGQSSALTIGETQQMNLVETTVELIEPNVESTKPAKGALTEAYGTAESNEETLLESLGLVPDDNRKIEQGKVNISEGEYVAKVQTTTVTDTEGEFVSVAPKLVNPKFDFVEQSALQIKQDTTVEKEEILSSNIELAPQLATSNMFPAELKVTSIYEVQPGLTSSDIITEQTKSVSANQVFETMSIGVTSKPDMLESTSHIDAFQHPEFKTGDTILDENQQPLEVTNVQITESSTDIIDVLPNQKLTKAETVTDGFKYAEGLVVLPMESTIDKTEDTKPTAVNADISMHQQFGTDVREQEPLESTLTRTEDLKPQTQTTESQFGLLQSLETSSCVTLEGESVLSVKERHPEQSAAIGTSSALQVANITRPQHMESLDRLDEQKVPYYQANVNIGEITLPNVEKIDSFDVLSDLNTPDYNKSSKGRVQLIESTTSLKTTTAVVSESTEELKDLNITQPVHIKPKPYESDQKISISEQTNVLEHVSSLNPVLPALETIQSSIKSLHEINVRETDILEKEESLKDVDHISGRLAKIILDCTTGIAQVRQEETLEHEEDLKAPLIPLEKAIPASSELHRLPLTEYVQEQQGTSDMTDFKVSNKCASPNIDHLYETKSSEMIVYDSSINSVDSEFPAGIVPQKSLVPFRHTMVTENVAFNASENFEILSADQQIATNVQDSLSQSIIAEDQIAFETEQNLGLETTPTHKPKLLKDDQNLHAKLVDEATVYEAMGQEQKVDKYNIQQAEITHDLPQVYATDLQQTFEAEKEITTREQSYVAATTDIIPSRLGLAMTTKTHPVEGIDVLLSSPPKPSLAQTNYEETQHEVRVRETQAIEESEELTDGRLLPVSAVESIDSTFKVTSDSQQPPVFDKELSIPTVSPLEARAKPSLNLLQGTTTFDVIPLESSVLLKDTHVAVQKAQQEYVAQVESNKVHVQMDNLVMHKEDIFENAEIENFCKPITEGTQLETVVIEVVPIDNVGGIHLAPQPSTLLATLTSTDIVNQSHVIDTQVPLEMESEAQAPLDNIAQARIKSAEDHVHTNVSEVNVYEQTKAIQDQNKHGLFVKVSKNSDTSKAYLTTIQSTFLKEDILPKPNILQDTAQAAADELQSLVTEEVVSVSSIQETYELKIPLQKTANLTQQTPQNSVNVCQQLAYEETPDIAFEPHALTRATTSSVPTFLKPAENATVNIYENIEGHGDFKPGTVNLTSNSNLNSELVVSVVQEVTSVPSLGSLATVEPQELKAMPVTKSSTNLAYSEEVKGNKQEFTKIETVEEDDKQPETTVTVEELPYEEEKPEEIQELPEEVCVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRFIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETAEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEENDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPKTTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDNQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDMQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEVEKPDEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKPEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTKIETVEEDDKQPETTVTVEEVPYEEEKLEEIQELPEEVRVVETVTEDGKPKKKKIRTRVIKKVKGDKQEVTTIETVEEDDKKAETTVTVEETELSAPSVGKVQLKKRVIVQKPEDAVTVFELPERKSVILSEKEDGTPTKTVIKTRIIKKIQGPNMEVTKVQTVEEYEKAPQTIVSVEKFNTPFPELPEERLSEVVMLPDEVFESEAVDEEGRLKMIKTKKRIIRKPALDNTEEVTEIGIIEQDNVEPIYSVKIQERPLTESKPEDSKLIELPEHVTELNVILPDGKKKRRTVKSRAFKKSLDDDLDEVTTIHIIEEEDKEPLTKVNIEVVPSDEISITPIPIEELPEETVFTEELDENKKPKKKTTKTRTFKKRGPDDDEYFQIQTIDEEGKEPISLIRVVSDENIADIIDISKLDDEKVLKHKQKPHKHKDQYYKEYTITEPEEASADALQKPTKDKTPKQKKTLETPIEEVDETVIIDEGTGEQTDQIAIKRKPRKVQGNVQVEAVDEKPIEKKEKAKKKKVVKTKRDEMDDYIQFLIHQEIPKTVLQPYQRTEMELPQRARRDSSFKQPVKLTPMKIEKVEFKKPKMVEISSVVEFPQMLKLKAPKQRPQEEKKKKNEASFKNKKLKSWIRFVPYAPYCFPYVVTELETNREVGELSRNVDEAEEVLKLRPKKFKHSKPEKAELEEADLGAYESDHSDKSNKELLHPKYKRGKKEKIETPDESRKLKFGKGKVPQNEEASEEVNLKPVKLDIAEIEDAEMPVRTQEEEVVKKKKPKKSSKPEEGLQFEPIEFEEMERTSDIREESDTSVSVDTSTQEKPIYKKKKKVTPSPQKNQYKILPGQPREFEETPEDDLNLRKRQGERPDDDKADTKLKPFYKFEVLDLEPEGVQAETVPLSETIAKEPKKKRKIKVKTEQEDNTIEIVPLSPEDNDEQIFEITVTSSEIPQGDAKAKTIGKKKVKRMNKQELDDFVTELQEEPNQEVYETRMSDFYEVKLTELPSEMDSDKPTKRILRHEKGDEVQVLEIVESVVAPGEEPFYEINVISSANTEGDSEEITTDKIKKKSRKIKKDDLDAYIQQLINAEIPVTELEKYEKIDVDGKAKKPKKLKAKTKKPIIDEGETLQVGVTEHEPTKKLKTKKPEEKKNVIEKELAEHEAVPEYDEFLINKTESERPQEKRVEETEKDIVPIVDKVLADLNDCLPFVVVEEDLKDMPLATDVIALEDEKIIRKRKVRAKKDSKQYEIEIIETEKPGDIPDEARVIVITTEVSGDTIDGPAPSTTEAPKKSVRKVKKEKLKEFIVNIVEEAPLDHVSEIYEDVLRTPFRESSEKEDIPSFTTTVVEDEIVNPVLPEKIKTVDDVRVPKDKKKKIDNQKKIKISEFEPTPTSEDSTIEEYTPKLSEHDEDLQTDEYSVDVKDSLPKSKKKSTKKQKKESLPGPISLYTIRIEETTPEPITEKIYEDGKEVVRVINKRRIKKKAGPKEYLIEVIETYEDNNPEADVVIRTIETTPSIDSKPQEDHKIQVVQEKKPKTESLDNYIQKLIDQEIPQVDHKEFKATVLETSPESKKAKKIKKHHKKTTEVIDGIPITVIEVTIQETETDDEDFKPDEVTLKEIDHENAEEAPKVLKSKVSEEKPKSKKEKSLEFKIAEEDKPKPVLEDISEDVQVVQIIEEDGTPKQVEIKKKKVSRKHGPKEQVFEITETKAIDEPLSEVTVVEITDEQPQEEVLPAQEKKPIKKQKKLKPEDVNTYVVKVLEELTEPTQFETIPEDADDKPQPVIEDISENVQVVQIIEEDGTPKQVEIKKKKVSPKHGPKEQVFEITETRPSDEPLAEVTIVELTEEGLNKDIVIPQEKKTVKKPKKLKPEDIQSYVIRVLEEFNEPQWPASTEKPIIEDIAESIEIVPVTEEDGITKEVEVKKKKVSRKQGTKNQVFEIIETKTSDEPLAEVTILELSGDKSQEVTILPKEKKPIKKTIKLKPEDVESYVVNVLEEFCEPQSFESPEPTEGEAHETKTKTKKPKKPIVKAPENVILIEEMAPETVIENIVNEIGEEVKQVKTTKKLKKKEGPKEYLIEIKETYEENKPEGDIEITTTELVPEGSPDASDDQPVIVVQKIKKKKPVKDDLDKYIQQLIEQEITKTPLEEYEPTEMDSKKKPKKKVKSHNKKTIEVIDGLPVTIHEFNVEDIVSEPEDMETPKTLLDEIKEIPQLPDDSSKYLVNISDEFGEADKPIKQPTQDQPIKKEKPLKKKKDVEYPVSLEAFDHTVKVVSEPTLEGTVKEVTVKKRKVSRRKGSKDHIFEITETTSEDRPTAEVTVVELSSDEVLDSEEKPKHERKIVKKPKQLKKDDVEEYIINIIEEFIQPIPVGLVEDEVEKVQKEETKKPKKSPITYIATEQEDNDNNYDALVKEDLDQPIERALEKPSSPLEYTISVEEDSVGEEQKQPKPKKISKPKSIKQPSVDKSPDYLVNVISEESIIDEPIPEDYVVTEAAEEKPSEEPTFKVEELETEAVEKEVTDDDKGETTKQSVTKRKIKKLVGPKEEIIEIVETKTGDTPEYEVIVTTEEVQEKSKEAPEEKKAKTVRKAKKIPKDDLQDYIQKLIEQDIPKTELEKYEKIDLDEPVKMKRKPIKKVKQSEEQPKEETEEPIEDKPVEKISEYSEVDSDEPKLTVAVKEFIPEKPEEKPFEIVVLEETVESKREPDEEGKVREKVVKTKKIKQNRGSVEVVHDIVEEIDTDTNESVITVTTTVPTETPDQDQPSVKQKRTKKIKKDEVEDFVKRVIEEEAPQPEGSVDLVVIEDFVPKPSSEKRKKKPIKDKHTSVEEETPHEDEVLLIESVPEDSPLSDDLITVVDSVPIEEEPENKVNQIEDTKKPEKKKKPKPSAKILEENVPEDTVEKPLEALHTDSDLEKPDVQEFSISIKEEEQKHTHPEKKKSSKISSEQPKQPSTEQYEISVTEHDLKPEEEKPFTVQVIQSETNVEETKDDTGKVHKQVTTKRMLRRPAGEGEIEIIEVVRDDQPEAEITIVEYEPEPVNQDEKPKEPKKKTRKVKKDDIHDYIQKLIELETPKTELEKYEKIEFEPIVKDKPLDSPIDVLDESPKEVQKKDKKSRSTKVPNEETPVQEQYAKVNVVEEEAPEQPEIPVQILEVKPVEVDVKEVITEDGKPVQEKTTKRVLKKIGPEEQTTFKITMIESEDNDSVTVIVDEEPEIASPQSIEEHPEQSKEKLAPKPKKTVRKVKKDDLSDYVKKLIEEEIPKVDLEKYEKVEMPEKPVKLTVSDSIPEEPKPDKSQPISVLPDTTKPKKTKTPKTPKTEDTDQQVPDEPTETTVDTTDIPELTPTQTAQPEDTATAQITPSAQEEKSTQDDTKDTIQKTVKHKKTKPDTQKSVETSELPEVHKDYQISIIHEELVEEEQPEKILEVRVIDEVAEVEESQPIVEEVEDEEPQPATEETVEDVTKPKSKKKKVVKKKTDDHDELIKKMLEQEIEKTELEKYEKIEFDVPKKLKPEFAALEPIKIERKEQKPTKVTILDATDVPKTVKLKPSKRKEKPAEELTVQLPKFRLKARMVLVEYPPAPLIPKTTDIGAIKDNGELSRNIEEAEEILKFKPHKTKKIKKIKDDLEKVELEKYEKYISSEEEPEEKTPYKKPEKAPKPEEKQEDVKLKLGKGKKKPKEEEAPENVTLKNIPQKPQEVEEEVELKQKPKEVEIVEEQTKKPKDGEFVVEPFEPSEFDRPEYVPDELEQIEHPEIPEKVKKPSKTKYKPKDKSKSEPETIVSEIVAGVPKEEEAIPEQDVKFRKPERDAPEETDSEIKLRPVPQASKDENPDEQALVTPKAEEPIPQEIEDKAIDDEKKPKKSKPKKVQPKEQEIAKEEPEEFEVSVKEEEALVDKPIEIEKPKDVKVKEKKPKEAPVSEVVVIEEEPKPEEVPEEIPVEYKITTTVLEPEDAPKEHQVKVIDFDERQETTEEVIEEKVVTRKKKPKPQQPEEFEVTLKEPKEEQIQPDVVSAEISLPIEEPEQKPEQYEVELKITQTTPEEPNDVQIAVKEKVKTKPVKKVKEDKIVVVEAEEEKQPVEETIVEVEKQEEKKKSEKPKSYEFKISETQSIEEKPIEVAEEAPEETPKVVEKKVAEKFDSYEFTLKETDEEKVITVDDQPEEEAPVEVVFKKKPKEPEAVEAEFVMTEPKIVEETSVETAIKQKKTKKPKKDEEEAQLAIKVVESEAPVAEEVFSEAPESKIVEEEVIAEEKPKEFTIRVSESEPKPEEPSVEQFTVKKRKPSVTFADEPATEIVIKESKPAEVVTEDAHIKTKKPKKKVTDVEAEELKIKITEEVPQEIPILEEVSEEEVITETKKTAPVVEEKTYKIGIKETEPEKPAEAIVEEEEPVVTEPIEEAPKPEVFEEHKVRVIEETPRELVEEVIEEEVKVIRRKKPKPEIKEEPEAEVTVSTPKPVEEVEATSSIAVIPEQPTEEEAADLKITIIEEETPPQELVQEIEEIEIVEEPKAPEEQPTDFTFATKDSEKKPTVEELPEEQVTIQKKKKKAPVPEVVEEPEAEFLVKPKTPVQEVTEEAKITKSKKPVKEEEAAAELKVTITEEIPTEPEVQEIIEEIEEIEEEKPAEYVIEVKESQPEAVEDKEVSLPKKKPKAPIVEEPEAEITLKPKVKSEEVQEEAKIVKKKPKKIDEVAVADELTVKVEEEVVPEPIVEEEVIEEFEIKKKPKEPEPEDIVDAAIVKLKKPEPVDADEVVAEVTLKPKAKTEVTEEEFSVDVKLPKEKKERPVEIEEEEIIEEAVVIRKKPKKPFEPTVEDLEETEFSLSFKKPHTINEGVEEAATVLKKRPVKPTTLDEAAAELSIKRQEEEYEEGEDIEEFVVSQQRKPKPLQITEEDEEAYTVKKLKRRKQVDIPEYADVENVTFRARSTKTKEDVDQEFNIALDSYAEEEISMSGKVKLKKPIKKTFSEAADEAKIKIIQDFDDGEEPIIEEIRDDEDTIDEVEEPEEYFVEELPPDEVDFKLKPKKHPKPAYSVQDEEEEQFLIGIRHPKRDSVTYDEDSLTFKKKRKVVQQLFNEDGASLNITREMNVEESENLNIMYSICNYIADNNEAINLVEGEKVTVVGRHSSEWWYVKKSTTEEEGWVPAQYLMEPEEYAQYVQNKLHEKIDKLPVFERPGPEDKPIAPRFIEKLQPIHTPDGYTVQFECKVEGNPRPQIAWFRETAIIKPSQDFQMFYDDDNVATLIIREVFPEDAGQFTVVAKNAAGFTSSTTELIVESPLSDHGSDATALSRRSMSRESSLADILEGIPPTFSKKPKAQYVDENTNVILECRLVAVPEPDIVWTFNGEDIDEEEIKNVRIVTESDMHMYCSVVHISKVKKSQEGTYEVIATNREGEARLPITLKVRTTDKEAPQILEPLRNMVIREGESVVLSTQIVGNPPPKVTWYKDGKPVKNAKSDKDLHTLTLITPQKSEKGEYTVKAVNPLGSVETTANLTIEEPAGGNAEPPLFVERFEEQNVPQKGEIRLPAKVSGNPVPEVQWLFNNTPLFPSERIQQVYDGENIELIIKDANPETDSGDYKCIASNPIGKTSHGARVIVEVDEVTFTKKLKKTITIEEVQSLTLECETSHVVTTKWFFNGKELSGMDHRVVVEDGKTHKLVIRNTNLRDSGTYTCKVKKQETQSTVEVLQRKPDFIKVLEDYEVTEKDTAILDVELTTEATEVTWYKDGEKITPENKNVEFIKDGKARRLVIRDVTIHDEGQYTCKIEGQECSCELVVIELPPEIVEPLNDVAVTKGENAVFEVELSKGDALVKWFKNGKEIVFNERIQLAIDGKKQSLRIVKAKPEDVGEYSVQVGEQTSKAKLTVEEPLVDFVIRLPDITLATKTTDAEFTVQLSQPDVEVTWCKKGKPIKPNQKHEVFVEGTVRRLVIHDASDEDAGEISCVAENVTSSTKLCVEELKLPPVITSDKDQTIKVKENDDVTFTVKYTGVPTPEACWTTRKVVIPKSKRTIPTIDEQSAKLTIKKVVDDDEGEYTVKLVNPVGEAEASLHLVIMRKPTAPGTPQPLEIMHDSITLYWKAPEDDGKSEIIEYILEYQDVKEEKWTEIRKIKDTTYTISKLKIDTEYVFRSIAVNEVGPSPPSPLSPPIRLVPKVETKAPSVQEPLQDVVSELDKEVTLSCVFGGIPEPKVTWKKNGQVFESRSIRYENRVAKYTIEKTTIETEATYTCVATNEKGSAETSCRLKLQQKPVLEVEDKYLTQKLRTGSILTIPATVRGYPQPTVTWHKETIEQKTTKSVTIETTETTSTYTVKKVTREQSGKYKVTATNESGTTYVECTVQVIDKPSRPQSLEIKDIKKDSIVLEWTPPVDDGGLDIEKYTLEKCDVQNNVWMKVSDFNKDIKSYAVQKLSMNAQYMFRVVAANPIGESEPTESDPVTITKKFEKPSPPRGPTTVSGMNDTSFNLAWEPSETDGGSKIIEYIVEIREETETTYRSVGVTLGTVTNIHVEKVVRNKGYFFRIYARNEVGTSEAFETTEKIVLGRKITPPSPPQNLRAPDVTSRSVTLDWEVPARNGGSEITGYCVEKRSSTSTNWTKVITLDAHQLHYTIDNLKEKCEYWFRVSAENEVGLGAPAVTESISLKTHASEFILVVRNCGSYTEFGFLKAVPSPPTGPLEARVLAANAHIFEWGLPESDGGAPLLGYHIAIRDMKKTMWIEVGRVPAGVLKFQIRDLQENHEYMIRIFAKNEIGLSEPLESEEPYKAMTAGHESLPDEPRTEMSSCNTSSWLRDHHMDADIHSYARGRLLQRDEYFFRLWAELPKSKKKKSSK