Protein: Transcription factor PDR1 (Pleiotropic drug resistance protein 1) (Q6FXU7)
Organism: Candida glabrata (strain ATCC 2001 / BCRC 20586 / JCM 3761 / NBRC 0622 / NRRL Y-65 / CBS 138) (Yeast) (Nakaseomyces glabratus)
Function: Master transcriptional regulator of a pleiotropic drug resistance network that contributes to the azole resistance of clinical isolates and petite mutants. Regulates the efflux of rhodamine 6G. Regulates both constitutive and drug-induced expression of the pleiotropic ABC efflux transporter CDR1. Commonly regulated genes include also those encoding ABC transporters PDH1, SNQ2, and YOR1, a phospholipid biosynthetic enzyme (PDR16), seven-transmembrane (TM) domain-containing proteins (RTA1 and RSB1), an oxidoreductase (YMR102c-like), a sphingolipid biosynthetic enzyme (IPT1), and a transcription factor (RPN4). Second, along with this common core of regulated genes, interesting unique genes including genes encoding an ABC transporter likely localized to the vacuolar membrane (YBT1), proteins involved in DNA repair (REV1 and MEC3), the mitochondrially targeted proteins YIM1 and PUP1, 3 oxidoreductases (OYE2, YIR036c-like, and YNL134c-like proteins), a major facilitator superfamily member (QDR2), a carbonic anhydrase (NCE103), an alcohol acetyltransferase (ATF2), and a transcription factor (HAPI). Activates transcription of target genes in response to direct binding to specific xenobiotics by a discrete transferable ligand-binding domain. Stimulates gene expression of target genes via binding to 5'-TCCGYGGA-3' elements called pleiotropic drug response elements (PDREs). PDR1 is recruited to the target promoters by mediator subunit GAL11A via its interaction with the KIX domain of GAL11A. Modulates the interaction with host cells in ways that may contribute to increased virulence. Regulates specific cell wall adhesins and in particular EPA1, explaining the increase in adherence to epithelial cells in gain-of-function mutants.
Subcellular location: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
Interaction partners:
- Candida glabrata strain CBS138 chromosome G complete sequence
- Pleiotropic ABC efflux transporter of multiple drugs CDR1
- ABC multidrug transporter SNQ2
- Pleiotropic ABC efflux transporter of multiple drugs PDH1
- Mediator of RNA polymerase II transcription subunit 15
- Lanosterol 14-alpha demethylase
- 1,3-beta-glucan synthase
Sequence:
MQTLETTSKSNPGEVKAQKPSTRRTKVGKACDSCRRRKIKCNGLKPCPSCTIYGCECTYTDAKSTKNLKSNDAGKSKPTGRVSKNKETTRVDKDIRKLEQQYVPINANIHVGPRFPSENILNGYPQCGAPQNNVVGNPLAVNTQCHRGLSETPMSSTFKESNLRDDRLLQSSDTDDMRNGDSEERDLKGSDSENVKSKDNKSDPLIIYKDDTHIESTVNKLTQAVNELKSLQNAPSSIKSSIDAIELQLRNILDNWKPEVDFEKAKINESATTKSLETNLLRNKYTNHVHLTRFRIWIDYKNANKNNHFMGECGFSLAESFFASNQPLVDELFGLYSQVEAFSLQGLGYCVHLYEPYMKTEEAIKLMKETLYIILRFIDICVHHINEESISIANPLETYLRKKHLMPMTPTPRSSYGSPQSASTKSLVSKIIERIPQPFIESVTNVSSLQLLDLRDDESKMFGTLLNMCKSIRRKFDSVMSDYDSIVTEKSEGEQNDGKVTVAEFTSLCEAEEMLLALCYNYYNLTLYSFFEFGTNIEYMEHLLLLLEEQLALDEYYGFEKVLNVAVANAKKMGFHRWEFYVGYEESTAEKRRLLWWKLYNYEKASTMKKGFFSVIDDATVNCLLPKIFRNFGYLDRVEFLENIQKPMDLSVFSDVPISVLCKYGELALTIVTSEFHEKFLYADRYTSIRNSAKPPTLKNQLIKEIVDGIAYTETSYEAIRKQTAKLWDIALGKVTKDKINKEDTAAASKFTLSYEYHRFRLINMADNLIARLMVKPKSDWLISVMKGHLNRLYEHWKVMNEIILSMDNDYSIATTFEYYAPSCLCLATQTFLIVRNMEMDDVKMMVAVYKRFLNLGMFLQSAKVCSLADSHTFRDFSRSFSFITIISRLMIIEFMQIKELTKVEFIEKFSEVCPDLADLPPMLLDPNSCLYFSLLQQIKKSGFTLSFKKILEDARMMDFNYDRNLDSEAIKKCNGEFSKSMPSCTNVSDTTTAVSDNSAKKKASMGSARVNSTDTLTASPLSGLRNQTQLDSKDSVPSLEAYTPIDSVSDVPTGEINVPFPPVYNQNGLDQQTTYNLGTLDEFVNKGDLNELYNSLWGDLFSDVYL