Protein: Bromodomain adjacent to zinc finger domain protein 2A (Transcription termination factor I-interacting protein 5) (TTF-I-interacting protein 5) (Tip5) (Q91YE5)
Organism: Mus musculus (Mouse)
Function: Regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair. Both complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template. Directly stimulates the ATPase activity of SMARCA5 in the NoRC-5 ISWI chromatin remodeling complex (By similarity). The NoRC-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the NoRC-5 ISWI chromatin remodeling complex (By similarity). Within the NoRC-5 ISWI chromatin remodeling complex, mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. In the complex, it plays a central role by being recruited to rDNA and by targeting chromatin modifying enzymes such as HDAC1, leading to repress RNA polymerase I transcription. Recruited to rDNA via its interaction with TTF1 and its ability to recognize and bind histone H4 acetylated on 'Lys-16' (H4K16ac), leading to deacetylation of H4K5ac, H4K8ac, H4K12ac but not H4K16ac. Specifically binds pRNAs, 150-250 nucleotide RNAs that are complementary in sequence to the rDNA promoter; pRNA-binding is required for heterochromatin formation and rDNA silencing.
Subcellular location: Nucleus, nucleolus {ECO:0000269|PubMed:11532953, ECO:0000269|PubMed:12198165, ECO:0000269|PubMed:16085498, ECO:0000269|PubMed:18600236, ECO:0000269|PubMed:19578370}. Note=Co-localizes with the basal RNA polymerase I transcription factor UBF in the nucleolus. {ECO:0000269|PubMed:11532953}.
Interaction partners:
- SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
- Histone-lysine N-methyltransferase SUV39H1
- Histone deacetylase 1
- Histone-lysine N-methyltransferase EZH2
- Transcription termination factor 1
- Polycomb protein Suz12
- Transcriptional repressor protein YY1
- Polycomb protein EED
- DNA
- SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 1
Sequence:
MEMEANDHFNFTGLPPAPAASGLKPSPSSGEGLYTNGSPMNFPQQGKSLNGDVNVNGLSTVSHTTTSGILNSAPHSSSTSHLHHPNVAYDCLWNYSQYPSANPGNNLKDPPLLSQFPGGQYPLNGILGGNRQPSSPSHNTNLRAGSQEFWANGTQSPMGLNFDSQELYDSFPDQNFEVMPNGPPSFFTSPQTSPMLGSSIQTFAPSQDVSSDIHPDEAAEKELTSVVAENGTGLVGSLELEEEQPELKMCGYNGSVSSVESLHQEVSVLVPDPTVSCLDDPSHLPDQLEDTPILSEDSLEPFDSLAAEPVSGSLYGIDDAELMGAEDKLPLEGNPVISALDCPALSNANAFSLLADDSQTSASIFVSPTSPPVLGESVLQDNSFGLNSCSDSEQEEIETQSSNFQRPLTEPAPDQPPSTQLHPAVSPTASPAASLTASAEISPAVSPVASSPVPPEVFVAVSPASSPALPAISLEASMTTPVTSPQGSPEPSPAAAFQTVSPARKNVSSAPKARADREETTGGAVAVSGSGDVLKRRIATPEEVRLPLQHGWRREVRIKKGSHRWQGETWYYGPCGKRMKQFPEVIKYLSRNVVHSVRREHFSFSPRMPVGDFFEERDTPEGLQWVQLSAEEIPSRIQAITGKRGRPRNNEKAKNKEVPKVKRGRGRPPKIKMPELLNKTDNRLPKKLETQEILSEDDKAKMTKNKKKMRQKVQRGESQTPVQGQARNKRKQDTKSLKQKDTKKKLKAEKEKMKTKQEKLKEKVKREKKEKVKAKGKEGPRARPSCRADKTLATQKRLEEQQRQQAILEEMKKPTEGMCLSDHQPLPDFTRIPGLTLSSRAFSDCLTIVEFLHSFGKVLGFDLTKDVPSLGVLQEGLLCQGDSLDKVQDLLVRLLKAALHDPGLPPYCQSLKILGEKMSEIPLTRDNVSEILRCFLMAYRVEPPFCDSLRTQPFQAQPPQQKAAILAFLVHELNSSTIIINEIDKTLESVSSCRKNKWIVEGRLRRLKTALAKRTGRPEVMMEGAEDGLGRRRSSRIMEETSGIEEEEEEENTTAVHGRRGRKEGEIDVAASSIPELERHIEKLSKRQLFFRKKLLHSSQMLRAVSLGQDRYRRHYWVLPYLAGIFVEGSEGSTVTEDEIKQETESLMEVVTSTPSSARASVKRELTGSNASTSPARSRGRPRKPKPGSLQPQHLQSTIRECDSEQAQTQVHPEPQPQLQAPTQPHLQPSSGFLEPEGSPFSLGQSQHDLSQSAFLSWLSQTQSHNSLLSSSVLTPDSSPGKLDSAPSQSLEEPEPDEAQSCPGPQGPWFNFSAQIPCDAAPTPPPAVSEDQPTPSLQLLASSKPMNTPGAANPCSPVQLSSTHLPGGTPKRLSGDSEEMSQSPTGLGQPKRRGRPPSKFFKQVEQHYLTQLTAQPIPPEMCSGWWWIRDPETLDVLLKALHPRGIREKALHKHLSKHKDFLQEVCLQPLTDPIFEPNELPALEEGVMSWSPKEKTYETDLAVLQWVEELEQRVVLSDLQIRGWTCPTPDSTREDLTYCEHLPDSPEDIPWRGRGREGTVPQRQNNNPLDLAVMRLAVLEQNVERRYLREPLWAAHEVVVEKALLSTPNGAPDGTSTEISYEITPRVRVWRQTLERCRSAAQVCLCMGQLERSIAWEKSVNKVTCLVCRKGDNDEFLLLCDGCDRGCHIYCHRPKMEAVPEGDWFCAVCLSQQVEEEYTQRPGFPKRGQKRKSSFPLTFPEGDSRRRMLSRSRDSPAVPRYPEDGLSPPKRRRHSMRSHHSDLTFCEIILMEMESHDAAWPFLEPVNPRLVSGYRRVIKNPMDFSTMRERLLRGGYTSSEEFAADALLVFDNCQTFNEDDSEVGKAGHVMRRFFESRWEEFYQGKQANL