Protein: NACHT, LRR and PYD domains-containing protein 5 (Maternal antigen that embryos require) (Mater protein) (Ooplasm-specific protein 1) (OP1) (Q9R1M5)
Organism: Mus musculus (Mouse)
Function: As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the formation of F-actin cytoplasmic lattices (CPL) in oocytes, which in turn are responsible for symmetric division of zygotes via the regulation of mitotic spindle formation and positioning. Required for the localization of cortical granules to the cortex of oocytes, via association with the cortical actin scaffold. Required for cortical actin clearance prior to oocyte exocytosis and prevention of polyspermy. Involved in regulating post-fertilization Ca(2+) release and endoplasmic reticulum storage (ER) storage via regulation of cellular localization. May be involved in the localization of mitochondria to the cytoplasm and perinuclear region in oocytes and early stage embryos, independent of its role in CPL formation.
Subcellular location: Cytoplasmic vesicle, secretory vesicle, Cortical granule {ECO:0000250|UniProtKB:P59047}. Mitochondrion {ECO:0000269|PubMed:14670992}. Nucleus, nucleolus {ECO:0000269|PubMed:14670992}. Cytoplasm {ECO:0000269|PubMed:14670992, ECO:0000269|PubMed:28992324, ECO:0000269|PubMed:31575650}. Golgi apparatus {ECO:0000250|UniProtKB:P59047}. Note=Located in mitochondria and nucleoli in primary follicle oocytes (PubMed:14670992). Colocalizes with ZBED3 and NLRP4F in the oocyte subcortex (PubMed:25208553, PubMed:28992324, PubMed:31575650). Not detected in oocyte mitochondria in type 2 follicles (PubMed:14670992). In the subcortical cytoplasm of early embryos from the 1-cell to the blastocyst stages (PubMed:14670992, PubMed:25208553). From the 2-cell stage, still detected in the subcortex, but excluded from cell-cell contact regions (PubMed:14670992). Expression largely disappears in blastocysts (PubMed:25208553). {ECO:0000269|PubMed:14670992, ECO:0000269|PubMed:25208553, ECO:0000269|PubMed:28992324, ECO:0000269|PubMed:31575650}.
Interaction partners:
- Oocyte-expressed protein homolog
- KH domain-containing protein 3
- Transducin-like enhancer protein 6
- Inactive protein-arginine deiminase type-6
- Growth/differentiation factor 9
- F-box and WD-40 domain protein 12
- Zygote arrest protein 1
- Zona pellucida sperm-binding protein 1
- Zinc finger BED domain-containing protein 3
Sequence:
MGPPEKESKAILKARGLEEEQKSERKMTSPENDSKSIQKDQGPEQEQTSESTMGPPEKESKAILKARGLEEEQKSERKMTSPENDSKSIQKDQGPEQEQTSESTMGPPEKDSKAILKARGLEEEQKSESTMSPSENVSRAILKDSGSEEVEQASERKMTSPENDSKSIQKDQGPEQEQTSETLQSKEEDEVTEADKDNGGDLQDYKAHVIAKFDTSVDLHYDSPEMKLLSDAFKPYQKTFQPHTIILHGRPGVGKSALARSIVLGWAQGKLFQKMSFVIFFSVREIKWTEKSSLAQLIAKECPDSWDLVTKIMSQPERLLFVIDGLDDMDSVLQHDDMTLSRDWKDEQPIYILMYSLLRKALLPQSFLIITTRNTGLEKLKSMVVSPLYILVEGLSASRRSQLVLENISNESDRIQVFHSLIENHQLFDQCQAPSVCSLVCEALQLQKKLGKRCTLPCQTLTGLYATLVFHQLTLKRPSQSALSQEEQITLVGLCMMAAEGVWTMRSVFYDDDLKNYSLKESEILALFHMNILLQVGHNSEQCYVFSHLSLQDFFAALYYVLEGLEEWNQHFCFIENQRSIMEVKRTDDTRLLGMKRFLFGLMNKDILKTLEVLFEYPVIPTVEQKLQHWVSLIAQQVNGTSPMDTLDAFYCLFESQDEEFVGGALKRFQEVWLLINQKMDLKVSSYCLKHCQNLKAIRVDIRDLLSVDNTLELCPVVTVQETQCKPLLMEWWGNFCSVLGSLRNLKELDLGDSILSQRAMKILCLELRNQSCRIQKLTFKSAEVVSGLKHLWKLLFSNQNLKYLNLGNTPMKDDDMKLACEALKHPKCSVETLRLDSCELTIIGYEMISTLLISTTRLKCLSLAKNRVGVKSMISLGNALSSSMCLLQKLILDNCGLTPASCHLLVSALFSNQNLTHLCLSNNSLGTEGVQQLCQFLRNPECALQRLILNHCNIVDDAYGFLAMRLANNTKLTHLSLTMNPVGDGAMKLLCEALKEPTCYLQELELVDCQLTQNCCEDLACMITTTKHLKSLDLGNNALGDKGVITLCEGLKQSSSSLRRLGLGACKLTSNCCEALSLAISCNPHLNSLNLVKNDFSTSGMLKLCSAFQCPVSNLGIIGLWKQEYYARVRRQLEEVEFVKPHVVIDGDWYASDEDDRNWWKN