Protein: E3 ubiquitin-protein ligase UBR2 (EC 2.3.2.27) (N-recognin-2) (Ubiquitin-protein ligase E3-alpha-2) (Ubiquitin-protein ligase E3-alpha-II) (Q6WKZ8)
Organism: Mus musculus (Mouse)
Function: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Plays a critical role in chromatin inactivation and chromosome-wide transcriptional silencing during meiosis via ubiquitination of histone H2A. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth (By similarity). Required for spermatogenesis, promotes, with Tex19.1, SPO11-dependent recombination foci to accumulate and drive robust homologous chromosome synapsis. Polyubiquitinates LINE-1 retrotransposon encoded, LIRE1, which induces degradation, inhibiting LINE-1 retrotransposon mobilization. Catalyzes ubiquitination and degradation of the N-terminal part of Nlrp1b following Nlrp1b activation by pathogens and other damage-associated signals: ubiquitination promotes degradation of the N-terminal part and subsequent release of the cleaved C-terminal part of Nlrp1b, which polymerizes and forms the Nlrp1b inflammasome followed by host cell pyroptosis.
Subcellular location: Nucleus {ECO:0000269|PubMed:14585983, ECO:0000269|PubMed:20080676}. Chromosome {ECO:0000269|PubMed:20080676}. Note=Associated with chromatin during meiosis. {ECO:0000269|PubMed:20080676}.
Interaction partners:
- E3 ubiquitin-protein ligase TRIP12
- Ubiquitin-conjugating enzyme E2 B
- Ubiquitin-conjugating enzyme E2 A
- E3 ubiquitin-protein ligase UBR4
- E3 ubiquitin-protein ligase UBR5
- Arginyl-tRNA--protein transferase 1
- ATP-dependent DNA helicase Q4
- Protein N-terminal glutamine amidohydrolase
- Testis-expressed protein 19.1
- Putative E3 ubiquitin-protein ligase UBR7
Sequence:
MASEMEPEVQAIDRSLLECSAEEIAGRWLQATDLNREVYQHLAHCVPKIYCRGPNPFPQKEDTLAQHILLGPMEWYICAEDPALGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHKLSSSEVVEEEDPLVHLSEDVIARTYNIFAIMFRYAVDILTWEKESELPEDLEVAEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCDQAKTVIVRNTSRQTKPLKVQVMHSSVAAHQNFGLKALSWLGSVIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFALRFAKNYRQLQRDFMEDDHERAVSVTALSVQFFTAPTLARMLLTEENLMTVIIKAFMDHLKHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDELRQKFLQGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMVQDWCALDEKVLIEAYKKCLAVLTQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPISRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQLFSTPDYGKRFSSEVTHKDVVQQNNTLIEEMLYLIIMLVGERFNPGVGQVAATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIESVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRENKEDTALPPPALPPFCPLFASLVNILQCDVMLYIMGTILQWAVEHHGSAWSESMLQRVLHLIGMALQEEKHHLENAVEGHVQTFTFTQKISKPGDAPHNSPSILAMLETLQNAPSLEAHKDMIRWLLKMFNAIKKIRECSSSSPVAEAEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDTSASATLDSSPPVSDAALTALGPAQTQVPEPRQFVTCILCQEEQEVTVGSRAMVLAAFVQRSTVLSKDRTKTIADPEKYDPLFMHPDLSCGTHTGSCGHVMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRSILSRRLNFSDQPDLAQWTRAVTQQIKVVQMLRRKHNAADTSSSEDTEAMNIIPIPEGFRPDFYPRNPYSDSIKEMLTTFGTAAYKVGLKVHPNEGDPRVPILCWGTCAYTIQSIERILSDEEKPVFGPLPCRLDDCLRSLTRFAAAHWTVALLPVVQGHFCKLFASLVPSDSYEDLPCILDIDMFHLLVGLVLAFPALQCQDFSGSSLATGDLHIFHLVTMAHIVQILLTSCTEENGMDQENPTGEEELAILSLHKTLHQYTGSALKEAPSGWHLWRSVRAAIMPFLKCSALFFHYLNGVPAPPDLQVSGTSHFEHLCNYLSLPTNLIHLFQENSDIMNSLIESWCQNSEVKRYLNGERGAISYPRGANKLIDLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGSLLCSQSYCCQAELEGEDVGACTAHTYSCGSGAGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCQERFRKIQKLWQQHSITEEIGHAQEANQTLVGIDWQHL