Protein: E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX5 (EC 2.3.2.27) (Hexose metabolism-related protein HEX3) (RING-type E3 ubiquitin transferase SLX5) (Synthetic lethal of unknown function protein 5) (P32828)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Component of the SUMO-targeted ubiquitin ligase (STUbL) complex SLX5/SLX8 that mediates ubiquitination and subsequent desumoylation of sumoylated proteins and proteins containing SUMO-like domains for their degradation. The STUbL complex SLX5/SLX8 stimulates ubiquitin conjugating enzymes, including UBC1, UBC4, UBC5 and UBC13-MMS2, and mediates the proteolytic down-regulation of sumoylated proteins. The STUbL complex SLX5/SLX8 is involved in ubiquitin-mediated degradation of histone variant CSE4, preventing mislocalization to euchromatin. The complex plays an essential role in maintenance of chromosome stability and links SUMO-dependent ubiquitination to a centromere-specific function during mitosis. The complex is involved in proteolysis of spindle positioning protein KAR9 and ensures correct spindle function by regulating levels of microtubule-associated proteins. During replication, the complex helps prevent DNA lesions via recombination and has a role in localizing the DNA damage protein DCD2. The complex especially ubiquitinates the nuclease YEN1 and prevents persistent accumulation of a fraction of YEN1 associated with sites of activity in late G2/M and helps maintain the balance between pro- and anti-crossover pathways during homologous recombination. It is also involved in ubiquitin-mediated degradation of DNA repair proteins RAD52 and RAD57. Along with SIR2, promotes silencing of genes at telomeric or ribosomal DNA (rDNA) loci. Finally, the complex is recruited to distinct genomic hotspots of non-H2B protein ubiquitination (ub-hotspots) by the sumoylated transcription factor-like protein EUC1 where it ubiquitinates EUC1 and presumably other targets.
Subcellular location: Nucleus {ECO:0000269|PubMed:18086879, ECO:0000269|PubMed:18948542, ECO:0000269|PubMed:23785440}. Chromosome {ECO:0000269|PubMed:31015336}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:26906737}. Chromosome, centromere {ECO:0000269|PubMed:23785440}. Note=Localizes to few distinct genomic hotspots of non-H2B protein ubiquitination (ub-hotspots). {ECO:0000269|PubMed:31015336}.
Interaction partners:
- E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX8
- ATP-dependent helicase SGS1
- Ubiquitin-like protein SMT3
- E3 SUMO-protein ligase SIZ2
- E3 SUMO-protein ligase SIZ1
- Ubiquitin-like-specific protease 2
- Double-strand break repair protein MRE11
- Ubiquitin-like-specific protease 1
- F-box protein MET30 subunit MET30)
- Cell division control protein 4
Sequence:
MHSDTNGRTKSNNSPSDNNPNETVILIDSDKEEDASIREANLPVRLYPDRRVGRRRDALNRFVRSDSRSRNSQRTHITASSERPDFQANNDDITIIREVGRFFGDDGPIDPSAHYVDLDQEPGSETLETPRTIQVDNTNGYLNDNGNNNESDDGLTIVEERTTRPRVTLNLPGGERLEVTATTTDIPIRRSFEFQEDLGASRRQLLRRSATRARNLFVDRSDENDEDWTDDTHNLPEAIQRARRESRMRMSRRIAERQRRVQQQRVSSDENISTSIRLQSIRERIQSYTPDIRSAFHRAESLHEFRSILQNVAPITLQECEEELMALFTEFRNQLLQNWAIDRVRNTQEEALRLHREALERQERTAGRVFHRGTLRESITNYLNFNGEDGFLSRLWSGPALSDADEERHTQNIIDMIQEREERERDVVMKNLMNKTRAQQEEFEARAASLPEGYSASFDTTPKMKLDITKNGKEETIIVTDDDLAKTLEDIPVCCLCGAELGVGIPDDFTGISQKDRGVSFEGLVSKYKFHCPYQTLARPSMLDRDLSKRTFIASCGHAFCGRCFARIDNAKKKSKMPKKKLAQLKGSAHPDNYGPKLCPADSCKKLIRSRGRLKEVYF