Protein: Deoxyribonuclease CdiA (DNase CdiA) (EC 3.1.-.-) (CdiA-Dd3937) (Toxin CdiA) (E0SDG8)
Organism: Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937))
Function: Toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. CDI modules allow bacteria to communicate with and inhibit the growth of closely related neighboring bacteria in a contact-dependent fashion. CDI is neutralized by its cognate immunity protein CdiI, but not by non-cognate CdiI from other bacteria. The C-terminal domain (CT) has strong DNase activity; this activity is inhibited by cognate CdiI.
Subcellular location: Target cell, target cell cytoplasm {ECO:0000269|PubMed:23469034, ECO:0000269|PubMed:25174572}. Note=In mixing experiments where target cells lack cdiI the DNase activity can be detected in target cells as a loss of DAPI staining (PubMed:23469034). Secreted to the cell surface by CdiB, its two partner secretion pathway (TPS) partner (Probable). Toxin translocation into the target cell depends on the proton motive force of the target cell, but not on tolA or tonB (PubMed:25174572). {ECO:0000269|PubMed:23469034, ECO:0000269|PubMed:25174572, ECO:0000305}.
Interaction partners:
- Outer membrane transporter CdiB
- Immunity protein CdiI
Sequence:
MAADTLMVTGAWLSNSGTLQGRQSVGLAVGRDFSQTADGVLTSGGTVTVTAGGVATAGALTAQGLALTAGRWRHQGAVTLGGDGRLVLDELDNGGTLRAGGAWDMQAAALSNGGTLQGGRLALTLSGAAVNRGTLAGERVTLTADSLDNGGTLLGMDALTLAIAGTARNQASGQWLSQGESRLTAGTLDNQGQWQGDSLSVTADRIRNAGQLLGLSALTLTADGTLTNTATGTLLTQGAAVLRAATVDNDGEWQAGRLRLTADSLRNGGRIQSDGALDVALSPAGVLTNTGTLAANGDTTLTPGGLDNRGAVSVRGDLTVTGTDLDNAGQLAARGALTLTGSYAGAGSLYSDAALTLRGTTLANDGGRWQGQTVDIGGGPLTNDGNITGLDSLTVTTTGALTNRGRLAGQTLGITADALDNAGTLLGVDALTLAIAGTARNQTSGQWLSNGAGRLTAGTLDNRGQWQGDSLDATADRLDNAGTLLGLSAMTLTVNGALTNTGRLLTQGAAVLSAATADNDGEWQTGSLWLTADSLRNGGQIHSDGEVRITLPTADGDPLRPTLRAARQLAQDVEAIGAGRLSNTGVLTAGGDGRITGRGLDNAGTLAAGGALTLAAGDLTNAGRLESRTLSLTGDSLDNGGTLLAEQGGELTLGGGLHVGADGRLLSNGDWQVQAGTVTSLGQWQGKTLLLSAASLDNGGALLATDAVTLTLTQGYTGGAGSQVLGSGAVTLTADTVTQQGDIGGDRLALTTGTLTNGGRLVGLSQLDVTSRGQLTNRATGSLLGNGTAGVTAATLDNAGSVQADTLTLTADTVTNAGRMQGTSALTLNGVSRYTGTDGSQLLSGGTATLAIDNADNAGLWQAGELRFRGASLTSRGQITGLDSLTVDAASLTSTGQLTTRGLATLRGQRFDNGGTLTALGGFTARFSDSVTNQGGGQLLSGGTGSLTTGTLVNRGRWQSDRLTLTADTLRNPGTLLGLDDGNIQLTGAYVGEAGSQVGGNGALSLSAATIDQAGQWQARDVTLRATRLRNQGSITGSGQLTATLDEQLENLAGATLLGGTVWLGGATVSNGGQIQGRSGLTVQGGTLLDNQGGGQLLSGGQLALGATQLTNAGWVQGQDLTLTTAQLDNSGTLQAQSGLTLHLPQWTNRGTVQAGQLDITTDGALDNRGTLLGLTRLALQAASLNNADGARLYSAGGLQLRTGQLTQDGQLAALGDLRADIGTPFTFTRTLAAGGQLTLAVTGDLVQAGTLQGHGVTVTSTGTLTQQGRIVAGGGNSTLSAAAISQTESGSIQGGGPLSLRATGNIVNRGFVGTAGDLLVQAGGVMENGSLLYGGGNLQLLSAALVNRFGNILAGGSLWIQRDAAGNASDSVLNSSGTIETQRGDITVRTGTLTNQREGLVVTESGSTAADMPDWVGGTTIYIPVERFEVIKDYLVYSFEHTPGAGSDSPTTYNYFYPFPLSHVSKQEFSASSKIVNIESKGGSSLIHSAGDINIFSSVLVNDASIIASEKNILMNGGVLKNSSYQSGVMSESLIYEYERDDKDDFLPYIEWLWEKTKREEGVSDYDYWEYLSGYNIHAYNRNILTNDRFKYVLKDRQIIFTPGQTYAATIQAGGAITANFSQNISNTNLQPGSGGFMPAMATPTLAGVNALGPVGAQADRGLNGGTAGNVSGSTLSGAGNGVALAGQAGRLNAGYSAVTRDNTASSGSALNPVGIPAGPGTAGGAPVAGASLTPVAPGALALSDLQAALAQGLQQLGSPSLTDYPLPTSQSGLFVADTAGDSRYLIRTNPTLSQLGQVDNALFGDLRGLLGQTPGTTAPVERSPTLTDPTQVLGSSYLLGKLNLDAEHDYRFLGDAAFDTRYISNAVLSQTGQRYLNGVGSELAQMQQLMDNAAAEKSRLNLQLGVSLTPEQVAGLSHSLVWWENITVGGQTVLAPKLYLAQADKTNLQGSRIVANSVSLSAGGDIDNRGSTVTAQDALAVASGGNLTNSEGGLLNAGGALNLVALGNLTNSSATIQGNTVTLASVGGDIVNTTTTDQWQTAARDGRGRGSLTRTDIGQAGLISAQGGLTLQAGHDIALNGAQLSAGGPLQLAAGNDIRLTALSTVTDTVRQDGGATTERRGQGLVQSTVASGGDLSLSAGRDLSGTAAQLSAAGTLALSAGRDLSLLSASEEQFSSNAWKRHLDWQQTVTQQGTVLNAGEGLSLRAGQDLTLQGAQAETRGALTAQAGRDLSLLSATESRHDFFEETTVKKGFLSKTTTHTLRETQQTTEKGTLLSAGSVALTAGHDIGVQGSAVAADGEVTLTAGNDITTAASVETYRNYEEQSRKKSGVFSGGGIGFTIGSTSLRQTLESAGTTQSQSVSTLGSTGGSVRLNAGQAVSMAATDVIAARDIQVTGNSVTIDPGYDTRKQSRQMEQKTAGLTVTLSGVVGSALNSAVQTVQAVREQSDSRLQALQGMKAALSGYQAYQGTQIDTNNQGASSFVGISVSLGAQRSSSSQTSEQSQSFASTLNAGHDISVVARQGDITAVGSQLKAANNVELNASRAINLLSARNTESMTGSNSSSGGNIGVSFGLSNSGAGFSVFANVNAAKGRELGNGNSWSETTVDAGQQIALTSGGDTRLTGAQVSGERIVANVGGDLLLKSQQDSNRYDSKQTSVSAGGSFTFGSMTGSGYLSASQDKMHSSFDSVQQQTGLFAGKGGYDISVGNHTQLDGAVIGSTAGADKNRLDTGTLGFSNIDNRAEFSVSHSGIGLSASPSLSMSDMLKSAALTAPSALMSMGRGGNAGSTTYAAVSDGALIIRNQAGQQQDIAGLSREVEHANNALSPIFDKEKEQKRLQTAQMVGELGAQVMDVIRTEGEIRAVRAAEAKGDVKRPPDNASEKDWDKYKKDLTETPAYKAVMQSYGTGSDLQRATQAATAAIQALAGGGNLQQALAGASAPYLAQLVKGVTMPADESKATASDIAANAMGHALMGAVVAQLSGKDAVAGAVGAAGGELTARLLIMKELYSGRDTSDLTEAEKQSVSALASLAAGLASGIASGNTTGAATGAQAGRNAVENNSLGDIAQAQSEGKTLEQNAGEYVEAENERYKKENCAGLSAEACSVKMYEERREELKETLSTGADFVPVIGDIKSFAEAQSALDYLAAAVGLIPGAGDAAGKAIKAAETALKKGELAEASKLINKASDEIQAVKPLDVGSYKELKDRAVVGDGLEHDHIPSFAALRTAKENELGRKLTPAEEKTLYQNATAVEVPKDVHRAGPTYGGKNTAAQVQQDALDLCGAVCRDTDALRTNMIERGYEPALVDDAVKKIIDRNRQIGVIK