Protein: ATPase histone chaperone YTA7 (EC 3.6.1.-) (ATPase family AAA domain-containing protein YTA7) (AAA-ATPase) (Tat-binding homolog 7) (P40340)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Functions as an ATP-dependent nucleosome disassembly factor that helps evict canonical histone H3 from the 5'-end of genes upon their induction. Also contributes to kinetochore assembly by cooperating with SCM3 to load the histone H3 variant CSE4/CENP-A at centromeres. Provides a chromatin boundary function at the 5'-end of genes that restricts access by RTT106 and thus prevents ectopic spreading of repressive chromatin into coding regions. Also prevents heterochromatin spreading downstream of the silent mating-type locus HMR, this function is independent of the tRNA boundary element. Contributes to appropriate cell cycle regulation of histone gene expression by recruiting RNA polymerase II to histone genes, and subsequent CDK1- and casein kinase II-dependent eviction from chromatin is required to promote transcriptional elongation.
Subcellular location: Chromosome, centromere {ECO:0000269|PubMed:32079723}. Nucleus {ECO:0000269|PubMed:32079723}. Chromosome {ECO:0000269|PubMed:19683497, ECO:0000269|PubMed:22156209, ECO:0000269|PubMed:25406467}. Note=Binds near the 5'-end within protein-coding genes (PubMed:19683497, PubMed:25406467). Localizes to the negative regulatory element NEG and promoter of the HTA1 gene during G2 and M-phase, and its open reading frame during G1/S (PubMed:19683497, PubMed:22156209). {ECO:0000269|PubMed:19683497, ECO:0000269|PubMed:22156209, ECO:0000269|PubMed:25406467}.
Interaction partners:
- FACT complex subunit SPT16
- Histone chaperone RTT106
- Histone H3
- Histone acetyltransferase SAS3
- Histone H4
- FACT complex subunit POB3
- Histone H2A.1
- Glycerol-3-phosphate dehydrogenase, mitochondrial
- Histone chaperone ASF1
- ISWI chromatin-remodeling complex ATPase ISW1
Sequence:
MARNLRNRRGSDVEDASNAKVGYETQIKDENGIIHTTTRSLRKINYAEIEKVFDFLEDDQVMDKDETPVDVTSDEHHNNNQKGDDEDDDVDLVSPHENARTNEELTNERNLRKRKAHDPEEDDESFHEEDVDDDEEEEEADEFEDEYLDEDSKDNNRRRRAADRKFVVPDPDDDEEYDEDDEEGDRISHSASSKRLKRANSRRTRSSRHPETPPPVRRALRSRTRHSRTSNEENDDENDNSRNEALTLADEIRELQEDSPIREKRFLRERTKPVNYKLPPPLTASNAEEFIDKNNNALSFHNPSPARRGRGGWNASQNSGPTRRLFPTGGPFGGNDVTTIFGKNTNFYNQVPSAFSDNNNNKLILDSDSSDDEILPLGVTPKTKKENTQKKKKKKPEIADLDPLGVDMNVNFDDIGGLDNYIDQLKEMVALPLLYPELYQNFNITPPRGVLFHGPPGTGKTLMARALAASCSSDERKITFFMRKGADILSKWVGEAERQLRLLFEEAKKHQPSIIFFDEIDGLAPVRSSKQEQIHASIVSTLLALMDGMDNRGQVIVIGATNRPDAVDPALRRPGRFDREFYFPLPDVKARFKILQIQTRKWSSPLSTNFIDKLAFLTKGYGGADLRSLCTEAALISIQRSFPQIYRSNDKLLVDPSKIKVKVSDFMLALKKIVPSSARSTGSSPQPLPELIKPLLADQLNNLKNKLDYMLNIKDTTFQRNTSLLQNFIDYEEYSGEEEEHDKYGGNEDTSSFRSYEFFESMAESQICKPRLLINGPKGNGQQYVGAAILNYLEEFNVQNLDLASLVSESSRTIEAAVVQSFMEAKKRQPSVVFIPNLDIWINTIPENVILVLSGLFRSLQSNEKILLLCLAENLDISEVKNGILSDFAFDKNIFQLHKPSKENITRYFSNLIELLKTKPSDIPMKKRRVKPLPELQKVTSNAAPTNFDENGEPLSEKVVLRRKLKSFQHQDMRLKNVLKIKLSGLMDLFKNRYKRFRKPPIDDAFLVHLFEPETSNDPNWQPAYIKDENMILEVSTGRKFFNMDLDIVEERLWNGYYSEPKQFLKDIELIYRDANTIGDRERVIKASEMFANAQMGIEEISTPDFIQECKATRQRDLERQELFLEDEEKRAAMELEAKEQSQENILQEPDLKDNKANEFGVAAGNQLQAQLQTTINTASIVNNSEVPQPIDTNLYKKEIPAAIPSAVDKEKAVIPEDSGANEEYTTELIQATCTSEITTDDDERARKEPKENEDSLQTQVTEENFSKIDANTNNINHVKEIQSVNKPNSLHETVEKRERSPIPKEVVEPEQGKKSDKELILTPEQIKKVSACLIEHCQNFTVSQLEDVHSSVAKIIWKSKSAWDKTGTVDEIIKFLSE