Protein: 1-phosphatidylinositol 3-phosphate 5-kinase fab1 (Phosphatidylinositol 3-phosphate 5-kinase) (EC 2.7.1.150) (Diphosphoinositide kinase) (Type III PIP kinase) (PIPkin-III) (O59722)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. The product of the reaction, PI(3,5)P2 is an important regulator of vacuole homeostasis perhaps by controlling membrane flux to and/or from the vacuole. PI(3,5)P2 regulates the transition between trans-SNARE complex formation and vacuole membrane fusion (By similarity). Required for survival under conditions of nitrogen starvation. May have a role in the secretion of pheromone peptides.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:11895483}. Note=During cell fusion of opposite mating types, migrates to the isthmus. {ECO:0000269|PubMed:11895483}.
Interaction partners:
- Protein VAC14 homolog
- Polyphosphoinositide phosphatase
- Phosphatidylinositol 3-kinase pik3
- Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase ptn1
- Phosphatidylinositol 4-kinase pik1
- Phosphatidylinositol 4-kinase stt4
- Phosphoinositide 3-phosphatase
- Phosphatidylinositol 4-kinase lsb6
- Uncharacterized protein C3C7.01c
- Uncharacterized protein C19F5.03
Sequence:
MSEVETPTAASPTFPVETSHRLDTLHTSSTEQIIKDSENVVHTTLKLPTSTLSREFWMKDERTNNCSLCETEFTLFRRKHHCRICGKIICKYCLKEAPGFIFRLQGSIKVCRPCASILVNNYSRSQLFNHSLNESKNRDLTEQHPFVTLDELNSNDQVLSSFGDLSSTFEMPNNIHPPEVAPMIAIPSSRSNYDSPGWAHHSIFLDWSKRNLDSNVINVEDSESGKYNALTITNSYDAGPSSVSTDYRPVNFGKVPSYSKLRKNKAFSSAKVSDMYLSADERNRLEDFSKGDRGLSFVNLSPNIKATSYDRLSTVINEPFISRSSSLTDERGLADSGNSYHHFSDSDDESLFNDGLGLSFHANSAIIKQRQQNVASIQRYGNESYLSNFLKAFLPKTVCDYLFPSSTIPDGLPALIENFNARVDKVNHPGGTEEPLPYQGKSRASSVVTSSKSTCILPPWILFSDSFNQLVCTFLGKLLFQMLNDEGVDSPMQWVLCLPKILLKMALDLGPDIRSGDDIDVRSYVKIKKIPGGSIQDCFLVNGVLFSKKASSKSMDRSLRRPRIALLTFSLDYACDEQRILSLDLIISQQEEYIINLVNRICMLKPNLVFAQGQIPSIALKYFEEHGVIAFHGLKESVLYDIARCCRADIISSIDKLSLCPRLGTCGRFQLRTYVVDENKGLRKTFAILDRCSERLGCTIVLRGADYNQLSKVKKIVELVVLIAYHIKLECALLRDKFVNMPELFETTYQSLSRKSLPSFASTAADKEKSQNHEKKSLNSDNQSLRPLENENQSVSSTQGSNSPLELINNLPASDDYSSITKALKTRFLTFSPFLSKPLPRLLNQVNYYQFIRNKLLKDVKLHPYSPTGSFVMKQSENDNVEESYEESYKFFCIDERYHFLEKQWTLYYSHSKLMFSPFSSQRIILLYSIINKETSVPCIGPERCLLEFYRETDCTLGQYIEDSCLNTNVSCGGEYCKTNDMLWHYRSYVHGNSRISVFLESFSCPVPGLEEKIIMWSYCKFCKKNTHITVMSEETWKYSFGKYLEFMFYNSQIRDRFEFCDHSVMAQHVHYFGYCNMALRFQRDLIEIFELFVPSVTLRNNPSYIKELKEKEYKRLKGVIEKCLSSVASRINQIKCDWVTDPEKFESCTSEISKFRTLLSSDYTELYSEFDSIYLNSSTSDYLSLNSILRVLQGKMVKWEQRFLDYQRLYLPSYKELSKIAAAQIKKVFLERPLSQTPLDLPETLENTQIDIYPSFKTESTDDQLEKVTQTNVASNKRVAPYADSMANVGSPESDCFSVATSSDIPKANIDFTNDISTQNTFPASPVSNSGFSRQTYPNISQRQGVNMLSHKRKSASTSDRRFVNASSTSGMNMPISSSISAKISSIQNSTKYSPRKPIPAKDVRVSSLVRRFEELSLQLQEKQKRDEELIKARRKRALPVVPSKPVVEVFNDLNEAFDDENSEDENGINDTKENRATESNFSGVDSMSKERENVSSNEDNSPEAFEDIFGILFKNESGLEEQQNLEPSSQMDKEGSKLPTSGPLADKTSVYRILSAFWNEWNSLNPPPFEFPLQPTEHMFSDSNVIIREDEPSSLISFTLSSPDYLSKMVEIEDSMDEALTNQGLQGSTQFKIENLMLKPTGTHLKYQFEEGSARLSCKVFFAEQFSALRRACGCEETFVTSLARCSLWESSGGKSGSAFLKTFDKKYILKVLSRLESDSLLNFAPAYFDYISKVFFHELPTALTKIFGFYRVDIRNPTTGTICKTDIMIMENVFYDECPSRIFDLKGSMRNRHVESTGKVDEVLLDENLVELIYESPIFVSEQLKSLLHSCLWNDTLFLSKLNIMDYSLIVGIDYTKKELYVGIIDFIRTYTWDKKLESWVKEKGLVGRGPEPTIVTPKQYKNRFRKAMDCYILASQDFETGEGFKFCE