Protein: Diacylglycerol kinase iota (DAG kinase iota) (EC 2.7.1.107) (F1MAB7)
Organism: Rattus norvegicus (Rat)
Function: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). Has probably no preference for any of the diacylglycerols in terms of the acyl chain composition, especially for the acyl chain at the sn-2 position. By controlling the diacylglycerol/DAG-mediated activation of RASGRP3, negatively regulates the Rap1 signaling pathway. May play a role in presynaptic diacylglycerol/DAG signaling and control neurotransmitter release during metabotropic glutamate receptor-dependent long-term depression (By similarity).
Subcellular location: Cell projection, axon {ECO:0000269|PubMed:21119615}. Cell projection, dendrite {ECO:0000269|PubMed:21119615}. Presynapse {ECO:0000269|PubMed:21119615}. Postsynapse {ECO:0000269|PubMed:21119615}. Postsynaptic density {ECO:0000269|PubMed:21119615}. Synaptic cell membrane {ECO:0000269|PubMed:21119615}. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000269|PubMed:21119615}. Cytoplasm, cytosol {ECO:0000269|PubMed:15024004, ECO:0000269|PubMed:21119615}. Nucleus {ECO:0000250|UniProtKB:O75912}. Note=Excluded from inhibitory synapses (PubMed:21119615). Localization between cytoplasm and nucleus is regulated by protein kinase C (By similarity). Both in the detergent soluble and particulate fractions (PubMed:15024004). {ECO:0000250|UniProtKB:O75912, ECO:0000269|PubMed:15024004, ECO:0000269|PubMed:21119615}.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm {ECO:0000269|PubMed:15024004}. Note=Not detected in detergent soluble fraction. {ECO:0000269|PubMed:15024004}.; SUBCELLULAR LOCATION: [Isoform 3]: Cytoplasm {ECO:0000269|PubMed:15024004}. Note=Not detected in detergent soluble fraction. {ECO:0000269|PubMed:15024004}.
Interaction partners:
- Lipoprotein lipase
- Dihydroxyacetone phosphate acyltransferase
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2
- RAS guanyl releasing protein 3
- Glycerol-3-phosphate dehydrogenase, mitochondrial
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4
- Phosphatidate cytidylyltransferase 2
- Ras-related protein Rap-1A
- Kinase non-catalytic C-lobe domain-containing protein 1
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3
Sequence:
MDAAGRGCHLLPLPAARGPARAPAASSALSPAGLCSGTASASSAAAGAVAMNPSSSAGEERGATGGSSSSGSGAGSCCLGAEGGVDPRGAGAAAAAALEEPAAAGQKEKEEALEEKLRDLTFRKQVSYRKAISRTGLQHLAPAHPLGLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGTEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQDLKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGLHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDQETSFPRAISAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISHDEIFILDPDMVVSQQAGTPPGMPDLVVEQASGLSDWWNPALRKRMLSDSGMITPHYEDSDLKDFSHSRVLQSPVSSEDHAILQAVITGDLMKLMESYKNGGSLLIQGPGHCSLLHYAAKTGNGEIVKYILDHGPAELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRQTDSKGKTPQERAQQAGDPDLAAYLESRQNYKIIGHEDLETAV