Protein: Vacuolar protein sorting-associated protein 41 homolog (S53) (P49754)
Organism: Homo sapiens (Human)
Function: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act in part as a core component of the putative HOPS endosomal tethering complex is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. Involved in homotypic vesicle fusions between late endosomes and in heterotypic fusions between late endosomes and lysosomes implicated in degradation of endocytosed cargo. Required for fusion of autophagosomes with lysosomes. Links the HOPS complex to endosomal Rab7 via its association with RILP and to lysosomal membranes via its association with ARL8B, suggesting that these interactions may bring the compartments to close proximity for fusion. Involved in the direct trans-Golgi network to late endosomes transport of lysosomal membrane proteins independently of HOPS. Involved in sorting to the regulated secretory pathway presumably implicating the AP-3 adapter complex (By similarity). May play a role in HOPS-independent function in the regulated secretory pathway.
Subcellular location: Endosome membrane {ECO:0000269|PubMed:21411634, ECO:0000269|PubMed:23322049, ECO:0000269|PubMed:25445562}; Peripheral membrane protein {ECO:0000269|PubMed:23167963, ECO:0000269|PubMed:25445562}. Late endosome membrane {ECO:0000269|PubMed:23322049, ECO:0000269|PubMed:33422265}; Peripheral membrane protein {ECO:0000269|PubMed:23167963, ECO:0000269|PubMed:25445562}. Early endosome membrane {ECO:0000269|PubMed:21411634}; Peripheral membrane protein {ECO:0000269|PubMed:23167963, ECO:0000269|PubMed:25445562}. Lysosome membrane {ECO:0000269|PubMed:21802320, ECO:0000269|PubMed:23322049, ECO:0000269|PubMed:33422265, ECO:0000269|PubMed:33851776}; Peripheral membrane protein {ECO:0000269|PubMed:23167963}. Golgi apparatus, trans-Golgi network {ECO:0000269|PubMed:23322049}. Cytoplasmic vesicle, clathrin-coated vesicle {ECO:0000269|PubMed:21411634}. Cytoplasm, cytosol {ECO:0000269|PubMed:23167963}.; SUBCELLULAR LOCATION: Note=(Microbial infection) Sequestrated at the late endosome by SARS coronavirus-2/SARS-CoV-2 ORF3A protein. {ECO:0000269|PubMed:33422265}.
Interaction partners:
- Vacuolar protein sorting-associated protein 33A
- Vacuolar protein sorting-associated protein 18 homolog
- Vam6/Vps39-like protein
- Vacuolar protein sorting-associated protein 11 homolog
- Vacuolar protein sorting-associated protein 16 homolog
- Syntaxin-17
- Transforming growth factor-beta receptor-associated protein 1
- Vacuolar protein sorting-associated protein 33B
- Synaptobrevin homolog YKT6
- Vesicle transport through interaction with t-SNAREs homolog 1A
Sequence:
MAEAEEQETGSLEESTDESEEEESEEEPKLKYERLSNGVTEILQKDAASCMTVHDKFLALGTHYGKVYLLDVQGNITQKFDVSPVKINQISLDESGEHMGVCSEDGKVQVFGLYSGEEFHETFDCPIKIIAVHPHFVRSSCKQFVTGGKKLLLFERSWMNRWKSAVLHEGEGNIRSVKWRGHLIAWANNMGVKIFDIISKQRITNVPRDDISLRPDMYPCSLCWKDNVTLIIGWGTSVKVCSVKERHASEMRDLPSRYVEIVSQFETEFYISGLAPLCDQLVVLSYVKEISEKTEREYCARPRLDIIQPLSETCEEISSDALTVRGFQENECRDYHLEYSEGESLFYIVSPRDVVVAKERDQDDHIDWLLEKKKYEEALMAAEISQKNIKRHKILDIGLAYINHLVERGDYDIAARKCQKILGKNAALWEYEVYKFKEIGQLKAISPYLPRGDPVLKPLIYEMILHEFLESDYEGFATLIREWPGDLYNNSVIVQAVRDHLKKDSQNKTLLKTLAELYTYDKNYGNALEIYLTLRHKDVFQLIHKHNLFSSIKDKIVLLMDFDSEKAVDMLLDNEDKISIKKVVEELEDRPELQHVYLHKLFKRDHHKGQRYHEKQISLYAEYDRPNLLPFLRDSTHCPLEKALEICQQRNFVEETVYLLSRMGNSRSALKMIMEELHDVDKAIEFAKEQDDGELWEDLILYSIDKPPFITGLLNNIGTHVDPILLIHRIKEGMEIPNLRDSLVKILQDYNLQILLREGCKKILVADSLSLLKKMHRTQMKGVLVDEENICESCLSPILPSDAAKPFSVVVFHCRHMFHKECLPMPSMNSAAQFCNICSAKNRGPGSAILEMKK