Protein: Multifunctional-autoprocessing repeats-in-toxin (MARTX) (EC 3.4.22.-) [Cleaved into: Actin cross-linking toxin F1 (EC 6.3.2.-); Actin cross-linking toxin F4 (EC 6.3.2.-); N-epsilon-fatty acyltransferase F2 (EC 2.3.1.-) (Rho inactivation domain-containing toxin F2) (RIDvc); ABH effector region toxin F5; Cysteine protease domain-containing toxin F3 (EC 3.4.22.-)] (Q9KS12)
Organism: Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Function: Precursor of a multifunctional toxin that causes destruction of the actin cytoskeleton by covalent cross-linking of actin and inactivation of Rho GTPases when translocated into the host cytoplasm. Upon translocation into the host cell, undergoes autoprocessing in cis mediated by the peptidase C80 domain (also named CPD domain): the protease activity is activated upon binding inositol hexakisphosphate (InsP6) present at the host cell membrane and delivers the Cysteine protease domain-containing toxin F3 chain to the host cytosol. The Cysteine protease domain-containing toxin F3 chain will then further cleave and release effector toxin chains that cause disassembly of the actin cytoskeleton and enhance V.cholerae colonization of the small intestine, possibly by facilitating evasion of phagocytic cells.
Subcellular location: [Multifunctional-autoprocessing repeats-in-toxin]: Secreted {ECO:0000269|PubMed:11032799, ECO:0000269|PubMed:15547287}. Host cytoplasm, host cytosol {ECO:0000303|PubMed:26185092}. Note=Secreted via the type I secretion system. {ECO:0000269|PubMed:11032799}.; SUBCELLULAR LOCATION: [N-epsilon-fatty acyltransferase F2]: Host cell membrane {ECO:0000269|PubMed:20212166, ECO:0000269|PubMed:22044757}. Note=Targeted to the host cell membrane via the membrane localization region (MLD). {ECO:0000269|PubMed:20212166}.; SUBCELLULAR LOCATION: [Actin cross-linking toxin F1]: Host cytoplasm, host cytosol {ECO:0000305|PubMed:19620709}.; SUBCELLULAR LOCATION: [Actin cross-linking toxin F4]: Host cytoplasm, host cytosol {ECO:0000305|PubMed:19620709}.
Interaction partners:
- Lysine-acyltransferase RtxC
- DUF6701 domain-containing protein
- TonB system receptor, putative
- 6-phospho-beta-glucosidase
- Flagellar motor switch protein FliN
- Acyl carrier protein, putative
- Acyl carrier protein
- Tol-Pal system protein TolB
- Protease, insulinase family/protease, insulinase family
- TonB receptor-related protein
Sequence:
MVFYLIPKRRVWLMGKPFWRSVEYFFTGNYSADDGNNNIVAIGFGGQIHAYGGDDHVTVGSIGATVYTGSGNDTVVGGSAYLKVEDSTGHLIVKGAAGYADINKSGDGNVSFAGAAGGVSIDHLGNHGDVSYGGAAAYNGITRKGLSGNVTFAGAGGYNALWHETNQGNLSFTGAGAGNKLDRTWSNRYQGSHGDVTFDGAGAANSISSRVETGNITFRGAGADNHLVRKGKVGDITLQGAGASNRIERTHQAEDVYTQTRGNIRFEGVGGYNSLYSDVAHGDIHFSGGGAYNTIIRKGSGNDFAKEGMTNAKADEIVLTKAVMSGSWIGQDHHVTAVKSASEPNTYLFAFADSTYTKINKVQLRNDPQTGELKYYSTAWYKEVNHLSNLANQDISDNGGFTAVNINGAYTLSDLKVEHQQSVTVHAVEKSLTEYEWVTYANGAVIDAKEVSLSDAKMGGHAIYADGTKVDVKAVKSNRQPNTYIYAKVLGPYTKIVVVELANDPETGALKYQARSWYKEGDHTANIANQDISSATGYNPMGKGGYSLSDLHYSVNAVRSTSETVADIEEYTDQTLFKPANDSGESSGDVRFNGAGGGNVIKSNVTRGNVHFNGGGIANVILHSSQFGNTEFNGGGAANVIVKSGEEGDLTFRGAGLANVLVHQSEQGKMDVYAGGAVNVLVRLGDGQYLAHLLAYGNISVQKGSGDSRVVMLGGYNTHTQIGSGNGLWLAAGGFNVMTQVGKGDVAAVLAGGANVLTKMGEGELTSGMLGGANVITHISNDDQLSNTTAVALGGANILTKKGKGNTLAVMGGGANVLTHVGDGTTTGVMVGGANILTKVGNGDTTGILLGVGNVLTHVGDGQTLGVMGAAGNIFTKVGDGTSIAVMIGAGNIFTHVGEGNAWALMGGLGNVFTKVGNGDALALMVAEANVFTHIGDGMSVALMLAKGNVATKVGNGTTLAAMVGNVNIFTHIGHGSTFAAMIGQANIMTKVGNDLTAALMVGKANIMTHVGDGTSLGLFAGEVNVMTKVGNGTTLAAMFGKANIMTHVGDGLTGVLALGEANIVTKLGDDFMGVVAAAKANVVTHVGDATTAAVLAGKGNILTKVGEGTTVGLLISDVGNVMTHVGDGTTIGIAKGKANLITKVGDGLGVNVTWGQANVFTQVGDGDRYNFAKGEANLITKVGDGQEVSVVQGEANIITHVGNGDDYTGAWGKANVITKVGHGQNVVLAKGEANIVTQVGDGDSFNALWSKGNIVTKVGDGMQVTAAKGQANITTTVGNGLNVTAAYGDANINTKVGDGVSVNVAWGKYNINTKVGDGLNVAVMKGKANANIHVGDGLNINASYAQNNVAIKVGNGDFYSLAVASSNTSSNKLSALFDNIKQTVLGVGGSQAINYLVQGDEASSSGTHKGRGAIATPEITKLDGFQMDAIKEVSSDLGDSLTGSVTKVDTPDLNKMQHALNVDDSSVQAPNLIVNGDFELGEHGWQSTHGVEASYAGSVYGVEGEGHGARVTELDTYTNTSLYQDLANLAQGEVIAVSFDFAKRAGLSNNEGIEVLWNGEVVFSSSGDESAWQQKNLKLTAQAGSNRIEFKGTGHNDGLGYILDNVVATSESSQQANAIREHATQNPAAQNALSDKERAEADRQRLEQEKQKQLDAVAGSQSQLESTDQQALENNGQAQRDAVKEESEAVTAELAKLAQGLDVLDGQATHTGESGDQWRNDFAGGLLDGVQSQLDDAKQLANDKIAAAKQTLSDNNSKVKESVAKSEAGVAQGEQNRAGVEQDIADAQADAEKRKADALAKGKDAQQAESDAHHAVNNAQSRGDRDVQLAENKANQAQADAQGAKQNEGDRPDRQGVTGSGLSGNAHSVEGAGETDSHVNTDSQTNADGRFSEGLTEQEQEALEGATNAVNRLQINAGIRAKNSVSSMTSMFSETNSKSIVVPTKVSPEPERQEVTRRDVRISGVNLESLSAVQGSQPTGQLASKSVPGFKSHFASTSIGIENELSGLVVVLPKNSAQTFGYVHDSQGNPLFMLTKDMNQGGYSNPVGINDIQGVNNWQTHTIELVTYPSEISDTAAVESRKEAMLWLAKEFTDHINQSNHQSLPHLVSDDGRFTLVISNSKHLIAAGNGTSIDAQGKTIGMTPSGQQATMAISAKEFGTSSSPEVRLLESAPWYQAGLRDEFLANAKNTTLDDPATAQNVYAYLTSVYSKTADLAKEYGIYINDWDPASEGFSPNAQGLTDPKVKNAWSILPRTKPVRMLELLSAEDSRYVRQQIAEKLKGTYSESLAKNVFEYFQYGGEVAGHGINNATTGSVQQPEPAILFEFRSVPSALSDFVPKTASTVKVDVKALDHFDSASRKAIITEVNALVSGSEDFDAWYQEYRASKGQPPVKNPKSSASANHKAEWLMTQHAEQWAKITAPYTDNHETLTSTKLASNDKEELHALGETSNLENNKQQENVASIINTMLNDMLPFYALRTERNLLVQEGDEGFEVRAWPGTEDKSKTIILEDPEDAAQHKAIERFILANFDNFEQMPDELFLVDNKVISHHEGRTHVLAQKVDGAWQYNATVELMSVTELLDAANVTGKIRGESYQQVIDALTDYHASITEHADYEPESVEKLLNLRKKIEGYVLGHPDSGRVEAMNSLLNQVNTRLDEVSLLSVAEQTIQAQNSFSRLYDQLEAANLKESKHLYLDQNGDFVTKGKGNLANIDLLGSREAVLEKVKLTVSNEYGQTVADTIFAGLSAKDLAKDGKGVDIAGLNKVHQAIEQHLSPVSATLYIWKPSDHSALGHAALQIGQGRTQLEGQAAADFNQQNYVSWWPLGSKSSNISNILNVATKDQPDLKLRWSDFSQPAHQNDTLEHDVASEENDGFGLHDGDIKLKRFIEKLNAAKGIDASFKEASEGYASVLLGNPDMLETTSIPAHVFQPFVEQWNDTSYDMMDVAHRFAQELRLQAQRSDDPELLEKRIGNVIRQFAERALEEIETFKASQADQGRVFRINLEGLDVAAMQAEWHRLSNDPDARYQLLTKNCSSTVAKVLKAGGADKLIGHTWLPKFGVWTPTELFNFGQALQEAQLEIAAKKQSHQVTDVLDALSGNEKPKENVAIENDGTPPRDKESLSPLTRFLNNELYGDKEARRKIGEITQTLLDHAVEKGESQKITLQGEAGRLTGYYHQGTAPSEGETSSPSGKVVLFLHGSGSSAEEQASAIRNHYQKQGIDMLAVNLRGYGESDGGPSEKGLYQDARTMFNYLVNDKGIDPSNIIIHGYSMGGPIAADLARYAAQNGQAVSGLLLDRPMPSMTKAITAHEVANPAGIVGAIAKAVNGQFSVEKNLEGLPKETSILLLTDNEGLGNEGEKLRTKLTASGYNVTGEQTFYGHEASNRLMSQYADQIVSGLSSSASVDEDLDQQGLDTTSTKDQGISNKNDHLQVVDSKEALADGKILHNQNVNSWGPITVTPTTDGGETRFDGQIIVQMENDPVVAKAAANLAGKHAESSVVVQLDSDGNYRVVYGDPSKLDGKLRWQLVGHGRDHSETNNTRLSGYSADELAVKLAKFQQSFNQAENINNKPDHISIVGCSLVSDDKQKGFGHQFINAMDANGLRVDVSVRSSELAVDEAGRKHTKDANGDWVQKAENNKVSLSWDAQGEVVAKDERIRNGIAEGDIDLSRIGVNNVDEPARGAIGDNNDVFDAPEKRKPETEVIANSSSSNQFSYSGNIQVNVGEGEFTAVNWGTSNVGIKVGTGGFKSLAFGDNNVMVHIGDGESKHSVDIGGYQALEGAQMFLGNRNVSFNFGHSNDLILMMDKSIPTPPLVNPFDGAARISGVLQGIATSGEGEDWLAAQEQQWTLSGAKKFVKDMSGLDQSSSVDYTTLVELDSQNERDSRGLKHDAEATLNKQYNQWLSGNGNSGTSQLSRADKLRQANEKLAFNFAVGGQGADIQVTTGNWNFMFGDNIQSILDTNLGSLFGLMTQQFTATGQAKTTFTYTPQDLPRQLKNKLLGQLAGVGAETTLADIFGVDYTASGQIVSRNGQAVDGVAILKEMLEVIGEFSGDQLQAFVDPAKLLDSLKAGIDMGADGIKSFAETHGLKEKAPEEEKDNSSVSVNGANVNSAQGATVADGNTETAETQDRAFGFNSLNLPNLFATIFSQDKQKEMKSLVENLKQNLTADLLNMKEKTFDFLRNSGHLQGDGDINISLGNYNFNWGGDGKDLGAYLGDNNNFWGGRGDDVFYATGKSNIFTGGEGNDMGVLMGRENMMFGGDGNDTAVVAGRINHVFLGAGDDQSFVFGEGGEIDTGSGRDYVVTSGNFNRVDTGDDQDYSVTIGNNNQVELGAGNDFANIFGNYNRINAGAGNDVVKLMGYHAVLNGGDGDDHLIATAISKFSQFNGGEGRDLMVLGGYQNTFKGGTDVDSFVVSGDVIDNLVEDIRSEDNIVFNGIDWQKLWFERSGYDLKLSILRDPSNDSDQSKFEHIGSVTFSDYFNGNRAQVVIGMSEKDLSGEREYTMLSDSAIDALVQAMSGFEPQAGDNGFIDSLESKSQAAISMAWSDVVHKKGLMV