Protein: Regulator of G-protein signaling loco (RGS) (Locomotion defects protein) (Loco) (Q9VCX1)
Organism: Drosophila melanogaster (Fruit fly)
Function: Acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). Confers GDI and GAP activities on G(i) alpha subunit Galphai. Confers GAP activity on G(o)-alpha subunit Galphao and G(i) alpha subunit Galphai. Involved in the dorsal-ventral axis formation of the egg. Acts as a G-protein signaling for glial cell differentiation during embryogenesis; Galphai, Galphao and the G-protein coupled receptor, moody, are required in the surface glia to achieve effective insulation of the nerve cord. May be essential for nurse cell dumping during oogenesis. Required in neuroblast asymmetrical cell division. Plays a role in stress resistance and life span control.
Subcellular location: Cytoplasm. Cell membrane. Apical cell membrane. Note=Recruited from the cytosol to the plasma membrane by the G(i) alpha subunit. Colocalizes with moody at the plasma membrane. Localizes as a crescent to the apical cortex in interphase and forms an apical crescent and segregates into the apical daughter cell at telophase. Colocalizes with Galphai and raps/pins at the apical cortex throughout mitosis.
Interaction partners:
- G protein alpha i subunit subunit alpha 65A)
- non-specific serine/threonine protein kinase
- G protein beta subunit 5
- G protein alpha o subunit subunit alpha 47A)
- LD33695p
- Chaperone Ric-8
- Guanine nucleotide-binding protein subunit beta-1
Sequence:
MHHHHPPLPITGASGSTAVGTGAAAAEDASPAANSGPAPISTSTTPSGSNSQQHQRRRKKRANYNYNGIRTVEVRRGYNGFGFTISGQQPCRLSCIISSSPAEQAGLRSGDFLISVNGLNVSKLPHETVVQLIGNSFGSIRMQIAENYYSDSSDEENAHATLRGQLLAASLRHKPRFLHHKAKLHRLRNSPQKKLNPPEAVEPHKSKSSPDHPTLKPVLEDPPLTANLSKAADVANVSAMVRAVGSAALECRVIVGYLGTIEMPKQISHSSKLQTVRSCIRKLRQEKRQPTIVLMCITPDSLSLQSSSGGVLATYSSARLNFVSSSSESENRFFGLVTSAVHNTQIEEEYEPSAGSAAAAGHISISHSCHVFVIDTKLIEHAQHLQRAHEFRLQCTRDPISNLCLEFPNNSEYVVNLVRSMYTMRILPPASRSHQADYEAGAGAAAAGGAVAAAHSPQPSNHSEISTTTSNSDSGIGFNNDCTNISDRILVVDFLAAGAGAAAAAHAGPPTYPAPARPLGIVGIPDNRLTVRAMPDHSALLRSPPASSSLRRPNLLASFNLIQSPATNLTSTRSCDDVLNLFVDDSPRTLAAVASMDDISLHSAAPSLDEGHTFAHPACVPRKMRRSLALETPTTPHKLSAQVFGQPGSRHSLGFEAIDSVQSSVSGACMDQTMDTWASLQNLHKSHKDRTSMSASSSSHCLLEATNSEPDLGNRALPANASPFRRAWGQSSFRTPRSDKVAKEQQQLGQSSPVRRTASMNASDNDMYIKTLMLDSDLKSSRSQHQLSLLQVPKVLTTPAPPSAITASVAAEGAAQDHGCPSSWAGSFERMLQDAAGMQTFSEFLKKEFSAENIYFWTACERYRLLESEADRVAQAREIFAKHLANNSSDPVNVDSQARSLTEEKLADAAPDIFAPAQKQIFSLMKFDSYQRFIRSDLYKSCVEAEQKNQPLPYSGLDLDELLKTNFHLGAFSKLKKSASNAEDRRRKSLLPWHRKTRSKSRDRTEIMADMQHALMPAPPVPQNAPLTSASLKLVCGQNSLSDLHSSRSSLSSFDAGTATGGQGASTESVYSLCRVILTDGATTIVQTRPGETVGELVERLLEKRNLVYPYYDIVFQGSTKSIDVQQPSQILAGKEVVIERRVAFKLDLPDPKVISVKSKPKKQLHEVIRPILSKYNYKMEQVQVIMRDTQVPIDLNQPVTMADGQRLRIVMVNSDFQVGGGSSMPPKQSKPMKPLPQGHLDELTNKVFNELLASKADAAASEKSRPVDLCSMKSNEAPSETSSLFERMRRQQRDGGNIPASKLPKLKKKSTSSSQQSEEAATTQAVADPKKPIIAKLKAGVKLQVTERVAEHQDELLEGLKRAQLARLEDQRGTEINFDLPDFLKNKENLSAAVSKLRKVRASLSPVSKVPATPTEIPQPAPRLSITRSQQPVSPMKVDQEPETDLPAATQDQTEFAKAPPPLPPKPKVLPIKPSNWGVAQPTGNYCNKYSPSKQVPTSPKEASKPGTFASKIPLDLGRKSLEEAGSRCAYLDEPSSSFV