Protein: Guanyl-specific ribonuclease pgl-1 (EC 4.6.1.24) (P granule abnormality protein 1) (Q9TZQ3)
Organism: Caenorhabditis elegans
Function: Guanyl-specific endoribonuclease which cleaves the phosphodiester bond in single-stranded RNA between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleotide, resulting in the formation of a corresponding 2',3'-cyclic phosphate intermediate. Together with the P-granule component pgl-3, is involved in the formation of P-granules. Together with pgl-3, probably recruits other granule components such as pos-1, mex-3 and glh-1 to P-granules. In addition, may act redundantly with pgl-3 to protect germ cells from excessive germline apoptosis during normal oogenesis and development of the two gonadal arms. This may in part be through regulating the localization of sir-2.1 which is involved in germ cell apoptosis. May protect somatic cells from excessive apoptosis during normal development. Essential role in male and female postembryonic germline development; maternally provided protein maintains a population of proliferating germ cells and zygotic expression is required for correct oogenesis.
Subcellular location: Cytoplasmic granule {ECO:0000269|PubMed:15238518, ECO:0000269|PubMed:19167332, ECO:0000269|PubMed:21402787, ECO:0000269|PubMed:24140420, ECO:0000269|PubMed:26598553, ECO:0000269|PubMed:27650246, ECO:0000269|PubMed:28806108, ECO:0000269|PubMed:32843637, ECO:0000269|PubMed:9741628}. Note=Localizes to P granules in germline precursor cells (PubMed:19167332, PubMed:24140420, PubMed:27650246, PubMed:28806108, PubMed:9741628). Localizes to P granules in germ cells at all stages of development except in spermatogenesis (PubMed:15238518). Co-localizes with pgl-3 in P-granules, but localization in P-granules is not dependent on an association with pgl-3 (PubMed:15238518, PubMed:21402787). At P-granules in the adult germline, co-localizes with deps-1 in the pachytene region and with prg-1 (PubMed:32843637). {ECO:0000269|PubMed:15238518, ECO:0000269|PubMed:19167332, ECO:0000269|PubMed:21402787, ECO:0000269|PubMed:24140420, ECO:0000269|PubMed:27650246, ECO:0000269|PubMed:32843637, ECO:0000269|PubMed:9741628}.
Interaction partners:
- ATP-dependent RNA helicase glh-1
- Guanyl-specific ribonuclease pgl-3
- ATP-dependent RNA helicase glh-4
- Argonaute protein wago-1
- ATP-dependent RNA helicase glh-2
- Piwi-like protein 1
- Uncharacterized protein
- P-granule-associated protein deps-1
- J domain-containing protein
- Argonaute protein hrde-1
Sequence:
MEANKREIVDFGGLRSYFFPNLAHYITKNDEELFNNTSQANKLAAFVLGASKDAPGDEDILEMILPNDANAAVIAAGMDVCLLLGDKFRPKFDAAAEKLSGLGHAHDLVSVIDDDKKLGMLARKAKLKKTEDAKILQALLKVIAIDDAAEKFVELTELVSQLDLDFDVYVLTKILGLISEETSDEVDIIRDNVVNAFDSCKPLLKQLMLDGPKSEPADPFISLLMDPLEESVGKVVNHIAQLFEEASKNEGDESLVLRSQLGYQLFFLIVRSLADGKREVSKKILSGIPTSVRAEVFPGLQRSVYKSAVFLGNHIIQVLLGSKKSFEDWDVVGVAKDLESAWKRRAIAELIKKFQVSILEQCFDKPVPLIPQSPLNNDAVIDNVNKALQFALWLTEFYGSENETEALGELRFLDSTSKNLLVDSFKKFVQGINSKTHVTRIVESLEKCCLSDTPSGRKSNVQPSTSQQQDSAYTKEEMTTVHNTYSVNTKAQVLNGLSDTNSSGLLVDSKDSLSLQEISCDEVDSSTLLSSSRNIGEGVTVKAVDPVPEKVNDAQQQQTVNEIEMASDANQDTSSSASPEVAPSFSTDGWDSPTKSVALPPGMQQIDEEETTVADKDSTPQPQARAETAWGSGDATPMPLPAPTNQYKVSGFGEAKVAKGFGQFAPTSSAYGGGGGRGGYGGGDRGGRGGYGGDRGGRGGYGGGDRGGRGGYGGDRGRGGYGGRGGRGGF