Protein: E3 ubiquitin-protein ligase rpm-1 (EC 2.3.2.33) (Pam/highwire/rpm-1 protein) (Regulator of presynaptic morphology protein 1) (Synapse defective protein 3) (Q17551)
Organism: Caenorhabditis elegans
Function: Atypical E3 ubiquitin-protein ligase which specifically mediates ubiquitination of threonine and serine residues on target proteins, instead of ubiquitinating lysine residues (By similarity). Shows esterification activity towards both threonine and serine, with a preference for threonine, and acts via two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates (By similarity). Mediates ubiquitination and subsequent proteasomal degradation of target proteins, including dlk-1. Negatively regulates a p38 MAP kinase pathway composed of dlk-1, mkk-4, and pmk-3 that functions presynaptically to regulate synaptic architecture. Has a role during synaptogenesis; regulates neuronal morphology, specifically presynaptic differentiation. May regulate the spatial arrangement of presynaptic terminals or restrict their development. By targeting both dlk-1 and mlk-1 for degradation, may negatively regulate mlk-1/mek-1/kgb-1 and dlk-1/mkk-4/pmk-3 pathways which are involved in axon regeneration after injury. Regulates axon termination in PLM and ALM neurons. May play a role in the formation of muscle connections between the body wall and the motor axons in the dorsal and ventral cord.
Subcellular location: Cell projection, axon {ECO:0000269|PubMed:10839353, ECO:0000269|PubMed:10839354, ECO:0000269|PubMed:15208641}. Note=Localizes in periactive zone along dorsal and lateral nerve cords. {ECO:0000269|PubMed:15208641}.
Interaction partners:
- F-box/SPRY domain-containing protein 1
- Mitogen-activated protein kinase kinase kinase dlk-1
- mRNA export factor rae-1
- PHR domain-containing protein
- Probable protein phosphatase 2C T23F11.1
- Mitogen-activated protein kinase pmk-3
- CCAAT/enhancer-binding protein homolog 1
- Nuclear anchorage protein 1
- Liprin-alpha
- Ras-related protein Rab
Sequence:
MSFFLREIDGERVCSTSTETSIPEDRKSARRLRKLLYKCAENTCKPDILRVPAHCGKLDKEEEKSKTPLDQQQQINFHDILNSKFVVGPPSPFALVAIAKSILARFGNPEEVSEKEDGSEEEAGTSGADEEGKQYKNTDQLVGASLTCIFEVLEQLSRRDAELCVQALESLLSLIQSMPIDCLQSENRLSMSAMMHVLKTLREDACPSVSSKATSCLVALSVACGEPEHLGSTIRSLICMKKNIRMSADSTYDMIQMPENLRKLLLKVRRKALGGDNTANSPPNWAMVDVHEHSVASSFSLPSLPDSSPSSDTPDDDNRIHSTMACDGTFLYILNYVGLYKLGTGLNETISGKLYAANQSLQSSKNVQMYLCNGSLYLRRNYSSCISVIDTDSLLDIGEVILPPSCVQHALFTDGTYFYSATLIANSTLSTIQLNDSFSPSNEPSSRRSHRLTDVKFTIQGDLQVPHQLPEFLPANLHPQTVDLHFTREMAFIQARSGKVYYAGNGTRFGLFETGNNWMELCLPEPIVQISVGIDTIMFRSGAGHGWIASVDDKKRNGRLRRLVPSNRRKIVHVCASGHVYGYVSENGKIFMGGLHTMRVNVSSQMLNGLDNVMISSLALGKSHGVAVTRNGHLFTWGLNNMNQCGRVESTSTTSSPRHSGRQEYQICPIGEHTWLTDTPSVCAQCGLCSARGVACGRVPRPKGTMCHCGVGESTCLRCGLCRPCGEVTEPAQPGRAQHVQFSSTAAPQRSTLHPSRVILSQGPHDVKVSSVSCGNFHTVLLASDRRVFTFGSNCHGQLGVGDTLSKNTPQQVILPSDTVIVQVAAGSNHTILRANDGSVFTFGAFGKGQLARPAGEKAGWNAIPEKVSGFGPGFNAFAGWIGADGDSSIIHSHTALLSSDNILKAQIVANKTNIFIFPREVGKDYIVIRRKLNVFEHHASDYKCWYTSWATDPKYDMLWYYNSAEMEIKGYDIFKKSEKSVGDAFDSLTFLAGAEFAVQVYDSPAYATSMSLGMQLLSATFSANVINLSEFWKEKHGEREQDHEKTIMDGYSVANRFDGTGGGWGYSANSVEAIQFKVSKEIRLVGVGLYGGRGEYISKLKLYRQIGTEADELYVEQITETDETVYDCGAHETATLLFSQPIVIQPNHWHVVSAKISGPSSDCGANGKRHVECDGVTFQFRKSAVSNNGTDVDVGQIPELYYQIVGGSESRDESDSNKQLSISRDMSNLFSPAALKNVTAEGIGNLLILLEWALQRVQIDEDTNNQVEGSAENQWSQERAGFVAILSMKLISRFVRTVYKEKGCHDEPGIDFANKLVNLHSMLLEFFFSTDMTGYENRPLIKKEEKVVEEGYTLMKCVSEAVKLFISLSHCFMGSRSLMNAHLIAVMNKGNHEALILTSAIIGSLAKIERFAHQLLCSTTTTERFPMLSSLLLKHFNCEKETLASLTSFPNILRFLYDQTFMRNAYENTSSLAEAILVKVSRDLAIPTDDTLMGPVVHQTSSRFRRRSAQPTWDMSDGCADAIAFRVDSEGIKLHGFGIYLPTEPDRRNFVGEIMMLSPDSSEKWTCLLRVTAEMSSEEKEVGIVRFPEYVLLSPGVTYAVKVNMMKNTKTFCGEGGVTQVHLLNGARLFFSGCSMSQNGTTVQRGQLPYLIYSILDQSNSLQIKQETIYDTFTLLLRLMANKIGAAITEGGALPACCQHLMSHINPHVMVYMERFPDKALEMMSTMEQLIPMVSNLNGVERVFHSYDSDDSGCDTPYSGIVTTVVESQHPYKPNTSSSMVLLFEEADYICVRFSPDCQTAQFDDQLTIYLKIDEHSYMPIERCYGSEWPSYPMILPGNCLMFVLDASSAVEGATSEQMFGYHVTVTGYLVGYNDSTMRLEQDLVWLSANACRIMTQLPINPSNIEHLSTAEDDTRHLFEKHGSLLKKGLSLSHSPTLSELCTKGQPPPAQSADLQFLREFLSGHTSTSAGFLAKWLPTGSVVDASKCQLSLSHDDLIVGKAVTLKLLCKDQYDREVDCPKLQVEVFASLGHRNPSSTIHQNLHIGNLPSSLLIHQNPFQPIIVNHTRYMNIAAMPAYANYSVEEIRLGFMIEELVKDRVPLKSSDSSLFSGTWTPTTAGKYRIECKVDGSDISHTYTVEVTERPHRAGKGTITKPSGSRRGAQMTVARTVSIPFSSDFSGIRMRLGTTLASTSVGVIPRGALVEFIEEMDNDDGKWIRLTDETALLYGCNQGVGQVWCLAYHRPLQRELIPLKADTDREKAVKLRRKEIEKESNGSKHHSVSIDAKETYILSPNDVLQVYSTPAPHSMIDGEKIIGPCDLMSSGWLANRHGVWIKLTGVEKYVLQKNDPSSETSLSFSTNGNDEEDLERPIERKKTRLPNALTPSVADCIRAVFAAFVWHEHLVKDLMAAAAYLRFHQNLHNIWQSCEIPSCTNAPAALQPIVKIWREICEVVETSVEQHLIMPPVSNKAMRAETVKPPSRSGGCELCDANITVPLTVHLRMAHPGCGGDCLGYGYNSNGKFTTGWSGECGAGGRGQSPWYLLCNTCRSQYLKKTPAGHHQERTRRWREFRFSTSASDARPEVIIRQNAMFLLDLNSRLQTESNSSSTATSGWTINLFPTHLSTPSTMPRSQQKRLDVPPNNSVHQNSYMKLGYSSDPGPKVNVIMSPPNQGADQSASLNRPGPSAINEPAEVLQSPSAALRTLFSNTNPSTSALLKRPVLAFCVEHHDLKRIKSACVQSVRRAVAFSHAFRVWNWLLRMVSSEYSVSDIILQYLTTLTSYNRLAEYMFSAKKNSNILPHPWRLCFLAGPIAADMVTQLHAFLHTVSIILQSAGVDGRLKSLCFKSWTLQLTAQEQDLLILTCNILGTVGGILSDTSILDSDNRFVKEMKDITKFADITASSRQAMVICLTDESGETFWESGEEDKNRSRSLSVQLDESAHGEILSLFIDNARDEGYRISSIAFKAILEDGRRKDLTSLTLESAYCGWLKCCIKDISHIQIQFKGPNPASRIRQLMILGYPAKTTGTPRLAPSTSHHLFFSDTQRDAFALFQAISSQAFCGELSEDDTLRERVIDLLFSRVQLYPLQNYVYTQVVQAMEKEVELLCDKSKRNYSYCCGLMSLLVRICDSRGNMDSFGQRNSVLTSITQLLIFSPVVVQRQCLNSLECIFASFTPSNVEVPKIIRNLLVVVGKVIQLQVRDKAAHTVVTVHLCSSVLNAPQNWRVDKSIDMDIGRQTAVLVENLCNGTYTPEWSNATKCELANCLLSLIQMPESVSYTETLSTGGKSKAVAVVSSKRFWTAISSLSLIKDKSWLELSERWKTVQDEEDQEPVLLCENHDDGHTVAQVFCVDCDVALCKECFTVMHLHKKNRNHGVKNLVQSSTQHDINIHQGCARMKFLNFLILFHGEALNGMVEVAADTLFPTSTSSIQPAMQSSSAFLGIHPMTCRFCGNNVPVEDQSLDGTCTHEDCVNYAKTACQVMHTCNHFCGGIRNEEECLPCMTCKREDAAQDGDDVCVICFTERLGAAPCIRLGCGHMFHFHCVRMILERRWNGPRIVFRFMQCPLCIQPIEHSGLQDLIEPLKTIRQEVVDKAKMRLEYDGLLTTPALTDPRSEFYNQPEEYALDRYMYVLCHKCKKAYFGGESRCQAALDSSQFNPEELLCGGCSDTSGVQVCPRHGVEYLEYKCRFCCSIAVYFCFGTTHFCAPCHDDFQRLMSLPKHLLPTCPVGPRSTPMEEQTCPLKMKHPPTGDEFAMGCGICRNISTF