Protein: Autophagy-related protein 2 (AtAPG2) (Protein PEROXISOME UNUSUAL POSITIONING 1) (F8S296)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Lipid transfer protein involved in autophagosome assembly. Tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion (By similarity). Binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source to the IM for membrane expansion (By similarity). Plays an essential role in plant nutrient recycling. Involved in the negative regulation of plant defense responses to biotrophic pathogens. Involved in a negative feedback loop that modulates NPR1-dependent salicylic acid (SA) signaling and limits senescence and immunity-related programmed cell death (PCD) in plants. Involved in the degradation of damaged peroxisomes.
Subcellular location: Preautophagosomal structure membrane {ECO:0000250|UniProtKB:Q96BY7}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q96BY7}. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P53855}; Peripheral membrane protein {ECO:0000250|UniProtKB:P53855}.
Interaction partners:
- Autophagy-related protein 9
- Serine/threonine-protein kinase ATG1c
- Serine/threonine-protein kinase ATG1a
- Serine/threonine-protein kinase ATG1b
- Autophagy-related protein 18a
- Autophagy-related protein 18b
- Autophagy protein 5
- Serine/threonine-protein kinase ATG1t
- Autophagy-related protein 11
- Ubiquitin-like modifier-activating enzyme atg7
Sequence:
MVFPWNIAKSAEEAFSRWAVKRVVKFLLKKKLGKLILGDIDLDQLDIQLRDGTIQLSDLAINVDYLNDKFDAPLVIKEGSIGSLLVKMPWKTNGCQVEVDELELVLAPRLESNKSSSNEASTSASTREDLHNIRLEIGKHENEMLMNAAKSASIDVHEGVKTVAKIVKWFLTSFHVKIKNLIIAFDPDFGKKQSEAGPRPTLVLRMTEIECGISEEQVSANEVSPDNFLGINRLANCVKFQGAVVELLNMDDDDDGDKTCDKKTSNDVTLIMTGVGGGFSGSLNFSIPWKNGSLDIRKVDADISIDPVEVRFQPSTIRWFLQLWKTFTSFGSDCFPSVSHSDFLTDSPTIPTNVMVTPPATLSLSGGQELEHDTTPNLQFIPDWFPSSFSKKEEDGEVDIGASVDQFFECFDAMRSYQSASGSQGMWNWTSSVFTAINAASSLASGSLLLPSEQQHVETSCKVSFAGVSVVLFFQDEVNWKGVSTRIHYLGAELRDISVSFQVCLHDLRLEGEVNSMEIADYCQGGNVVDTANAESQTCLIKDLQAKVQTSLPPFASSDMHSDSERLSEIVSDGFLFRNKGFAVKTLLVIAAGGSGFQFTVNFQSSKASHRGSNSFSLSLPPTTFWLNLHSVEMLVNLFNDVSESIPITSHERNQVASSSKSESLRGSVSICNARVILWFPFESISERFCNSLGQQFIVVDLSSSPPSDKERAKERSPGEMHFPSATRSICFSVGDASIYLVTSDLKDSETNSYHRQVEFSAYNILHTNNKTRHQLSTIGMFWQDRPTVSPWLVERAKMLATQEESIQTDKSGGRGLEFAAVATPKDQDDIYSRSRKEIILASSFCLYVHLLPLAIHLDSWQYSKLCNLIEEAKNWLSRMAANTAEQTEESVVCQTSLVVDCDSIDILVRPEPRMGIKKQLQTELPGSWIQFNLRVQKLNLMSVPNLGSVSGADFFWLAHGEGTLLGSVTGLPDQELLLLSCNNSAIKRGNGGGSNALSSRFAGLDFLHLQEPGICNDYLAVSARGCTISAVGGRLDWIEVATSFFSFEDEKKTQEINSSSSSGSSFILNFVDVGLSYEPHHENTDHLRQASDPWVACLVAASSFSLSKKSLVDSIRNDYRIRIQDLGLLLSVDFDLSKLGGTYSSEHLHESGYVKVANDSLIEAILRTNSENGLLWELECSKSHLVIETCSDTTSGLIRLATQLQQLLAPDLEESAVHLQTRWDSIQQANARNDLDISDRLSSSDSSGEMKYLRLESENETGVIGLMDEINEDAFQFDVNPTYQSDSVECQNNYMSPHGISHGQAYNWVPATEKLPSNQSICGSSSRINSESSQVFLERESLPEIFENYCLSEFRPSSEVPQEGDSSGRELFPETDLRRGNSGWYDDASLRIVEDHVSEATEEDHEEHILDGECSSFGQTSYSAVAANGRILLKNIDLKWRIYSGSDWHDSRKKGENFKHTKGRDTTSCLELELSGVQFLYETFPIGEICTSKLSLMVQDFYLYDRSDNAPWTLVLGYYNSKDHPRDSSSYAFKLELKAVRPDPETPLEENRLRVALLPILLHLHQSQLDFLISFFGANSLEKPVVSMGDSGGSTMSVSVQGHNIIEEALLPYFQKFDIWPVNVRVDYSPHHVDIAALTGGKYAELVNLVPWKGIELQLKHVHAAGIYGWGNVCETILGEWLEDVSQNQIHQLLKGIPTVRSLSALYAAALKLVSSPVESYRKDRRLVKGVQRGTVAFLRSISLEAVGLGVHLAAGAHDILLRAEYIFASSPSLPQPQGRTKTNVRHNQPRNAKQGMLKACESIGDGIGKTASALVRTPLKKYQRGDGAGSAFATVVQGVPTAAIAPASACARAVHSALVGIRNSLDPEHKKESMEKYLGPDKQRKQDQHR