Protein: Tudor domain-containing protein 6 (P61407)
Organism: Mus musculus (Mouse)
Function: Tudor domain-containing protein involved in germ cell development, more specifically the formation of chromatoid body (during spermiogenesis), Balbiani body (during oogenesis), germ plasm (upon fertilization), and for proper miRNA expression and spliceosome maturation (By similarity). Essential for RNA-dependent helicase UPF1 localization to chromatoid body, for UPF1-UPF2 and UPF1-DDX4 interactions which are required for mRNA degradation, using the extended 3' UTR-triggered nonsense-mediated mRNA decay (NMD) pathway. Involved in spliceosome maturation and mRNA splicing in prophase I spermatocytes through interaction with arginine N-methyltransferase PRMT5 and symmetrically arginine dimethylated SNRPB (small nuclear ribonucleoprotein-associated protein).
Subcellular location: Cytoplasm {ECO:0000269|PubMed:17141210, ECO:0000269|PubMed:19345099, ECO:0000269|PubMed:27149095, ECO:0000269|PubMed:28263986}. Note=Present in chromatoid body (CB) of spermatids, also named processing bodies (P-bodies) in somatic cells (PubMed:17141210, PubMed:19345099, PubMed:27149095). Detected in the multilobular cytoplasmic CBs (also called intermitochondrial cementin) in pachytene spermatocytes and as a single perinuclear CB in haploid round spermatids (PubMed:17141210, PubMed:19345099). Colocalizes in CB with DDX4, PIWIL1, PIWIL2, TDRD1 and TDRD7 (PubMed:17141210, PubMed:19345099). {ECO:0000269|PubMed:17141210, ECO:0000269|PubMed:19345099, ECO:0000269|PubMed:27149095}.
Interaction partners:
- Piwi-like protein 1
- Piwi-like protein 2
- Tudor and KH domain-containing protein
- Piwi-like protein 4
- ATP-dependent RNA helicase DDX4
- ATP-dependent RNA helicase TDRD9
- ATP-dependent RNA helicase DDX25
Sequence:
MSSTPGLPTPGASLALRVSFVDVHPEVIPVQLWGLVGQRREEYVRLSREIQEAAATRGPWALGGASASPGELCLVQVGLMWHRCRVVSRQAQDSRVFLLDEGRTITAGAGSLAPGRSEFFHLPSEVLGCVLAGLVPAGGGGTGGGEPQQWSPRAVDFLSNLQGKEVHGRVLDVLLLHRLVLLEVPVVSQQMEELGLARQVPDSLFCSLLKRYLTAAGQGSSGAPVLPRAAPKQEHPGLDYFYPQLQLGVTEPVVVTQVCHPHRIHCQLRSLSQEIHRLSESMAQVYRAPVGTDDEDSGSATWEEREESPDKPGSPCASCGLDGQWYRALLLETFRPQRCAQVLHVDYGRKELVSCSSLRYLLPEYFRMPVVTYPCALYGLWDCGRGWSRSQVGDLKALILGQAVNAKIEFYCSFEHMYYVTLYGEDGINLNSAFGVQSCCLADWFLQSQGIEEEEEEDEDEVEAAFQSQSPAEEMEAEVSLPSLRSIRLKMNTFYDAQVEFVKSPSEFWIRLRKHKNTFSKLTKRMCSFYSSASKLDGVILRPEPDDLCCVKWKENGYYRATVTRLDSKSVDVFLVDRGNSENVDWCDVRMLLPQFRQLPILALKCTLADIWPLGKTWSQEATSFFKKTVLHKELVVHVLDKQDHQYVIEILDESRMGEENISKVIAQAGFAKFQEFETKENIRLSAHSPGHVSGHFMAEPSKITSAKKAEGDQRAKKDNKTLSVSEALADTVSLSNLSTAQDTEKVTSDPSLLMLNFLKTKPDCCGKGELEVGSTVEVKVSHIENPGSFWCQLMRNAQGFRTLMCDIEDYCKSSEPSPYEGDTRVCLAKRTASGRWSRALISGAHSLEHVRVVFVDYGDRDVVSTKDILSVSDVFFQVRAQAFRCSLYNLIQPMGENPFVWDEKAVQAFSGFIDSARQNNLELKCTVFALASRHEEEWFNVVDLLTPFQSACRFLVEKRLARPVKHQKPLEPSVQLHSYYYSTHDLKIGSEELVYVTHADDPWTFYCQLARNINVLEQLSYNIMQLSKALLNLKASTLAPGTLCLARYTDGNWYRGIIIEKEPSKVFFVDFGNTYIAVDHLLPIPRDAHDVLLLPMQALKCSLSDIPHHIPEEVTAWFQETVLDKSLKALVVAKDPDGRLIIELYDDSVQINASINEKLGLLGYKNRTRRKEKENEIILHETKALEDKKESVKPSLADYLGKPGESKAHSIEIMGESCKPKMGPACKELRYLQGSAKANLVPPYQDSVGNKNDGGFPLTREKKEDIFASSPMSGTKLDSALPERRMGEPSGRDLPPKFCEFPQKTIAPGFKTSVYVSHINDLSDFYIQLIEDEAEINNLSERLNDVRTRPQYHTGPQWQSGDVICAVFPEDNLWYRALVMEQQPNGLLSVQFIDYGNMSVVHTNRTGRLGPVDAVLPALCLHCSLWGLSVPVCKEMVSYFSQRTDEAQIRCEFVKFQGTWEVILADEHGVIAEDMISRFPCNGNSQAGLTTQTMKGDCLKIANKPNTDTSVLLNWYNPKAKLIKAYATVIDGPEYFWCQFADSEKLQYLETEVQSAGKQLSDRRSCTQCPQIGDPCIVRYREDGHYYRALITNICDGELASVRLVDFGNAEDCVDAKELWSIPSELLLVPMQAFPCCLAGFSVSGGVCPQEGNDYFYDIVTEDVLDITILEIKRDVCNIPLAIVELRSKGENINEKMKKYAKTGVPKNDLSSEKRGPERKGSLASPDLGLKKPSHKIAQDKTFYGEARASELSERLEKDLNIETKTSKFYERSTRSIFNAFENSCKGKMGSERLEGSMDYHFVDRAKFDNNYLITGFNPILAHASEPKELLELSSLEVPLSADNDDECKEFLELESIELQHSPAGEEEKEELGLGSPMAPLSPGCQAGATLESFMMQLPLDCEAEKQLELKLPTPQLSLEDSISPLSAAVSQDIQGSRCSEDERKAGYMGSSDDDHSRSPLLQHGKGGNSPAHDGRNLSEEEFPQFESRDSAALLAPLFSEEEAREGRKCGSMVPAQLQSTYTLKGFSVGSKCVVWSSLRNTWSKCEILELAEEGTRVLNLSNGVEETVSPENVWNGIPKVDKRPSEAVFQTVGKDLPFMPSDDATTKGFSSVSEEEACGGDADSLSTAKLNI