Protein: Enhancer of filamentation 1 (hEF1) (CRK-associated substrate-related protein) (CAS-L) (CasL) (Cas scaffolding protein family member 2) (CASS2) (Neural precursor cell expressed developmentally down-regulated protein 9) (NEDD-9) (Renal carcinoma antigen NY-REN-12) (p105) [Cleaved into: Enhancer of filamentation 1 p55] (Q14511)
Organism: Homo sapiens (Human)
Function: Scaffolding protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. As a focal adhesion protein, plays a role in embryonic fibroblast migration (By similarity). May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRKL and SHPTP2 to the tyrosine phosphorylated form. Promotes adhesion and migration of lymphocytes; as a result required for the correct migration of lymphocytes to the spleen and other secondary lymphoid organs. Plays a role in the organization of T-cell F-actin cortical cytoskeleton and the centralization of T-cell receptor microclusters at the immunological synapse (By similarity). Negatively regulates cilia outgrowth in polarized cysts (By similarity). Modulates cilia disassembly via activation of AURKA-mediated phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin. Positively regulates RANKL-induced osteoclastogenesis (By similarity). Required for the maintenance of hippocampal dendritic spines in the dentate gyrus and CA1 regions, thereby involved in spatial learning and memory (By similarity).
Subcellular location: Cytoplasm, cell cortex {ECO:0000269|PubMed:11827972}. Nucleus {ECO:0000269|PubMed:29899023, ECO:0000269|PubMed:8668148, ECO:0000269|PubMed:9584194}. Golgi apparatus {ECO:0000269|PubMed:8668148}. Cell projection, lamellipodium {ECO:0000269|PubMed:24574519, ECO:0000269|PubMed:8668148}. Cytoplasm {ECO:0000269|PubMed:11827972, ECO:0000269|PubMed:9584194}. Cell junction, focal adhesion {ECO:0000269|PubMed:19103205, ECO:0000269|PubMed:19275884, ECO:0000269|PubMed:29191835, ECO:0000269|PubMed:8668148}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:11827972}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:16394104, ECO:0000269|PubMed:9584194}. Cell projection, cilium {ECO:0000269|PubMed:17604723}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000269|PubMed:17604723}. Basolateral cell membrane {ECO:0000250|UniProtKB:A0A8I3PDQ1}.; SUBCELLULAR LOCATION: [Enhancer of filamentation 1 p55]: Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:9584194}.
Interaction partners:
- [F-actin]-monooxygenase MICAL1
- Aurora kinase A
- Breast cancer anti-estrogen resistance protein 3
- Dedicator of cytokinesis protein 3
- Proto-oncogene tyrosine-protein kinase Src
- Crk-like protein
- Mothers against decapentaplegic homolog 3
- Focal adhesion kinase 1
- Adapter molecule crk
- Protein deacetylase HDAC6
Sequence:
MKYKNLMARALYDNVPECAEELAFRKGDILTVIEQNTGGLEGWWLCSLHGRQGIVPGNRVKLLIGPMQETASSHEQPASGLMQQTFGQQKLYQVPNPQAAPRDTIYQVPPSYQNQGIYQVPTGHGTQEQEVYQVPPSVQRSIGGTSGPHVGKKVITPVRTGHGYVYEYPSRYQKDVYDIPPSHTTQGVYDIPPSSAKGPVFSVPVGEIKPQGVYDIPPTKGVYAIPPSACRDEAGLREKDYDFPPPMRQAGRPDLRPEGVYDIPPTCTKPAGKDLHVKYNCDIPGAAEPVARRHQSLSPNHPPPQLGQSVGSQNDAYDVPRGVQFLEPPAETSEKANPQERDGVYDVPLHNPPDAKGSRDLVDGINRLSFSSTGSTRSNMSTSSTSSKESSLSASPAQDKRLFLDPDTAIERLQRLQQALEMGVSSLMALVTTDWRCYGYMERHINEIRTAVDKVELFLKEYLHFVKGAVANAACLPELILHNKMKRELQRVEDSHQILSQTSHDLNECSWSLNILAINKPQNKCDDLDRFVMVAKTVPDDAKQLTTTINTNAEALFRPGPGSLHLKNGPESIMNSTEYPHGGSQGQLLHPGDHKAQAHNKALPPGLSKEQAPDCSSSDGSERSWMDDYDYVHLQGKEEFERQQKELLEKENIMKQNKMQLEHHQLSQFQLLEQEITKPVENDISKWKPSQSLPTTNSGVSAQDRQLLCFYYDQCETHFISLLNAIDALFSCVSSAQPPRIFVAHSKFVILSAHKLVFIGDTLTRQVTAQDIRNKVMNSSNQLCEQLKTIVMATKMAALHYPSTTALQEMVHQVTDLSRNAQLFKRSLLEMATF