Protein: Pre-mRNA-processing-splicing factor 8A (PRP8 homolog A) (AtPRP8A) (Protein ABNORMAL SUSPENSOR 2) (Protein EMBRYO DEFECTIVE 14) (Protein EMBRYO DEFECTIVE 177) (Protein EMBRYO DEFECTIVE 33) (Protein SUPPRESSOR OF OVEREXPRESSED FCA 81) (Q9SSD2)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex (By similarity). Required for embryo development (Ref.3). Required for splicing efficiency of COOLAIR introns and usage of the proximal poly(A) site. COOLAIR is a set of long non-coding antisense transcripts produced at the FLOWERING LOCUS C (FLC). COOLAIR initiates just downstream of the major sense transcript poly(A) site and terminates either early or extends into the FLC promoter region. Splicing of COOLAIR by PRP8A is functionally important for FLC regulation.
Subcellular location: Nucleus {ECO:0000305}.
Interaction partners:
- SNW/SKI-interacting protein
- Pre-mRNA-processing factor 17
- Pre-mRNA-processing factor 19 homolog 1
- Zinc finger CCCH domain-containing protein 4
- Pre-mRNA-splicing factor
- AT3G18790 protein
- Tetratricopeptide repeat
- Pre-mRNA-splicing factor SPF27 homolog
- P-loop containing nucleoside triphosphate hydrolases superfamily protein
- Cell division cycle 5-like protein
Sequence:
MWNNNDGMPLAPPGTGGSMMPPPPAAHPSYTALPPPSNPTPPVEPTPEEAEAKLEEKARKWMQLNSKRYGDKRKFGFVETQKEDMPPEHVRKIIRDHGDMSSKKFRHDKRVYLGALKFVPHAVFKLLENMPMPWEQVRDVKVLYHITGAITFVNEIPWVVEPIYMAQWGTMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNLLDVDPLEPIQLELDEEEDSAVHTWFYDHKPLVKTKLINGPSYRRWNLSLPIMATLHRLAGQLLSDLIDRNYFYLFDMPSFFTAKALNMCIPGGPKFEPLYRDMEKGDEDWNEFNDINKLIIRSPLRTEYRIAFPHLYNNRPRKVKLCVYHSPMIMYIKTEDPDLPAFYYDPLIHPISNTNKEKRERKVYDDEDDFALPEGVEPLLRDTQLYTDTTAAGISLLFAPRPFNMRSGRTRRAEDIPLVSEWFKEHCPPAYPVKVRVSYQKLLKCYVLNELHHRPPKAQKKKHLFRSLAATKFFQSTELDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREILRLTKLVVDANVQFRLGNVDAFQLADGLQYIFSHVGQLTGMYRYKYRLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAPMWRVWLFFLRGIVPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDVLDAMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPVPIENMILRYVKSKADWWTNVAHYNRERIRRGATVDKTVCRKNLGRLTRLWLKAEQERQHNYLKDGPYVTPEEALAIYTTTVHWLESRKFSPIPFPPLSYKHDTKLLILALERLKESYSVAVRLNQQQREELGLIEQAYDNPHEALSRIKRHLLTQRGFKEVGIEFMDLYSYLIPVYEIEPLEKITDAYLDQYLWYEGDKRHLFPNWIKPADSEPPPLLVYKWCQGINNLQGIWDTGDGQCVVMLQTKFEKFFEKIDLTMLNRLLRLVLDHNIADYVSAKNNVVLSYKDMSHTNSYGLIRGLQFASFVVQFYGLLLDLLLLGLTRASEIAGPPQMPNEFMTFWDTKVETRHPIRLYSRYIDKVHIMFKFTHEEARDLIQRYLTEHPDPNNENMVGYNNKKCWPRDARMRLMKHDVNLGRSVFWDMKNRLPRSITTLEWENGFVSVYSKDNPNLLFSMCGFEVRILPKIRMTQEAFSNTKDGVWNLQNEQTKERTAVAFLRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALIGLMTYFREATVHTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVIFYTPKEIGGLGMLSMGHILIPQSDLRYSKQTDVGVTHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAQAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDVIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKYKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESVVMDLCQVLDQELDALEIETVQKETIHPRKSYKMNSSCADVLLFAAHKWPMSKPSLVAESKDMFDQKASNKYWIDVQLRWGDYDSHDIERYTRAKFMDYTTDNMSIYPSPTGVMIGLDLAYNLHSAFGNWFPGSKPLLAQAMNKIMKSNPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGEIFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKVIHTSVWAGQKRLGQLAKWKTAEEVAALVRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKIEKFGDLILKATEPQMVLFNIYDDWLKSISSYTAFSRLILILRALHVNNEKAKMLLKPDKSVVTEPHHIWPSLTDDQWMKVEVALRDLILSDYAKKNNVNTSALTQSEIRDIILGAEITPPSQQRQQIAEIEKQAKEASQLTAVTTRTTNVHGDELIVTTTSPYEQSAFGSKTDWRVRAISATNLYLRVNHIYVNSDDIKETGYTYIMPKNILKKFICVADLRTQIAGYLYGISPPDNPQVKEIRCVVMVPQWGNHQLVHLPSSLPEHDFLNDLEPLGWLHTQPNELPQLSPQDVTSHSRILENNKQWDGEKCIILTCSFTPGSCSLTSYKLTQTGYEWGRLNKDNGSNPHGYLPTHYEKVQMLLSDRFLGFYMVPESGPWNYSFTGVKHTLSMKYSVKLGSPKEFYHEEHRPTHFLEFSNMEEADITEGDREDTFT