Protein: DNA-dependent protein kinase catalytic subunit (DNA-PK catalytic subunit) (DNA-PKcs) (EC 2.7.11.1) (Q54UC0)
Organism: Dictyostelium discoideum (Social amoeba)
Function: Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Is recruited to DNA ends by the Ku70/Ku80 heterodimer and is involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination (By similarity). This activity is only apparent when DNA damage is administered in G1 phase of the cell cycle. Required for efficient signaling of DNA double-stranded breaks via phosphorylation of H2AX during G1.
Subcellular location: Nucleus {ECO:0000250|UniProtKB:P78527}. Nucleus, nucleolus {ECO:0000250|UniProtKB:P78527}.
Interaction partners:
- SAP domain-containing protein
- X-ray repair cross-complementing protein 5
- ATP-dependent DNA helicase ku70
- DNA ligase 4
- HIT domain-containing protein DDB_G0272839
- DNA 3'-5' helicase
- Protein raptor homolog
- Protein pianissimo A
- Protein LST8 homolog
- BRCT domain-containing protein
Sequence:
MDNIYQRIENYLIKLFQTISETNNNNNNNNTNNNVGIKFENDLLLVNELGSIILKELVLEEEIGLVASLLFTGDHSLLKYLEKSSTISNKENVKIKVSILNLIAEFIEILQVRTDDYAIAIKNTCVLVFRKDQSHSVQAAAFGPIKKILHLMSRVLKQGSIVAESFGVSEMTQLFLLQFTCGKLTQTVRGEIIVVLGLFTEYFSSNMCDRNQQLSFIFMETLGGQLRSKSPESTLIQSCLKGLNSLLVHFSGDFIASDPKNVQLIYQYVYICLDPASSTQRFEIPRAAMKIVARHAVLLRQYLAEHSQPFYTRIEHWCNHINKLNRDIAFTAVDSFFQQISKELTSGNRSLEADQSTFKFFIRKFYSIFENNNSSRFELSIAIRGCGRFASPVKSFMGEWELKSLLNSLFKFSEKLMVVKIENIDEITLHLSSFINAFACILYELNELEFWYLDHIEQVLETYFIIFPHLFVKSRDRYFKAVNRLISSLFYHGEYLKILLSRFVKKGLLITFSKPNDSIKNLITTANTPFYQVYKDLWYNLLNPTIDTIDKDLNSTIKKENNNNNNNKNKNNNNNQTLTKEEISKSIKKLVYDEIVSSILSIIKKLDLQYQKDENDNNSNSNSNNNNNNDQDNNKISIIEYESEINQLKPVTSKDIELFLGLVEFFKLFISKYNTELFVQWIYIFGKEMISFSKKYPLISGFYKLVQIVMQICKSQKYFNELQQDDNINSVIFNNQIIDEQLQQQQDIMDIDDTDNNSNSNNKINKKLTNSLEEDKNNCFILFKKYIKEVSNFISHYKDELLSSCIELLLSLPKQLISIPLLVPIANLAFQYGISYLPLAHVGLNAIEYWNLVVPDQVHKHLDQLLPSLNDYLKISTNSSDSTSGGDIDIDSGGSMGGGGVVPPPSSSSRHRKMKFKSNSSLIDPKQLQFKTSIEDLQNRIIRLLGQLGGDNVHFLGKVSLVGEEIVNGVAWDTEPKVLIKIPFADQNNIDIYLDVILPNVVNLAEKSPNRQIKVAAGELLHSVLLMMIGNSANQSQANQISYSRLYRKIFPSLICLSTDVEQVTKQLFQPLVFQMIHWFTRNKKQESDDTMNLLNAIVDAVGNPLDGARRDFAGQCLCEFAKWSLKNTSVKQQEKNAFNFKSILKRIYSLAHHPDPYKRLGAAISFNQLYRIFREEDSLVDQFIFEILHNIFFSLRIGDDLTNTSSSKNNSNSNSNNNNNNNNNSEDGTEDSIVEIASKYSNVLAALTKVIIKRADMLNKPSNTRREHKDLSQFVQWVFQNGCSRTESIVRYESMILFTQLVTLLPNIKTPLQWVKERIKSNGIQFIVSIAEQQGGGVGGGSSNGNTSLGRIPKIEIGKHKDIEFWFRNLSTSLNIYLWFFSEGFFEPIQILTSREFKSSLLSSAFHTFTTQFSMLNFNSNLKTNQQQQQQQVSMFSSMTPKEIDQFNRLKCNVITNLFGLYILLLEKYKFEQVIDYGGVAFVQLLVKCLMDPQSVGFVNNYLEEREVELSKSSRQDIRNLMSRTFKFPPLLEIINNIIKLLIQIKPKYNDILHKELMEYILPNNSNNNNNSSSSGNNKSMFSLIDIRQLINTEGTDRLIHLIHSYQILYKYNLLDSILLENYNEQSEQGIQDLPKSSLEFSEFIFDYLFNNCENLSPSKLLISKELIQLALTIGIKPIKLLSLIINPNVQNTTTTTTTTSTTTTTTTTTTSTNNNNDNNNEFIKDIHDIFYKTLFTEINNYISNNFQLFLPLLANRIIETNQIHKVLNDVCIMKQQQQPNRGFKDIIESIVLFLNSISEWTLNSNLKLVEKENIIEFTKNLIKIDPIQFFENKHCYEFVYNIITNYLCRSNPLTFKNKVLVLLPYLLSYPKHNNFDLIKQKLNEIVVYDFPLNSKDLTVKSPIYNEYITCIERLLETFELTKNPLVIDTLLHVLKETDHTHINYINLSIERSILSTNDIEAKEIFSHCFELFLNHYDELKITLIDKFCVPLISHMKENILVQIFSQHLSSLMSIIQPLQPKYLSDSQERKSSIIEKICCFHLIEALYQSLPSAIIKEKINPFFYDKPDGKGTELTAAIMKAAHSAKSEKLTSDDKYVTRSLCTKYHGAAFSALASVIVSTQTKENFFHVFFFKENKDKNEYLWENIIDTDQVFNFQPETNFLVSYSTPQSLFANDLRYLSSQYLVDSSLSQDFIAKNFLEGDNNSTTNNNNGDDGIIMQGQSQSGDNENQISNDIEIDQVNSNPSMIAMLKIIDFYQKKFVVTPPSSILFGQQSQQPKPTVGDMPKWMFEIYQKLQQGVHPNIVIFMIKIIINRPQYFERFHELWIPILMDYVVSEGNGGNGLHYFVRDVCFILLKWPNIYKSQDGGGGTTLEKHLSKFVNHLMKNTYCTDRRILKSNLNIVKSFVERWKSLFKVDKSIILELLSTKGDFKSKSRQIKTTGLVLLSILLSNGYPAYDKEYDSNTISEFKFYQVLLDHLQDFKELYDAASEVCGMILLYLSKQQQSQQQQQQSIFPQLLKDKINSILNNNENQRAFSCLYFIGLHYPLFLQGFYGKIFNLLPQISNETRLIALNIIHWCVEDIQDLYTKLKSNNIENLIRIREGEIQVVILRILYKLVQKRDTTFGTVNQILSLLLSNNWFTSNVTNEYSRSLYYDIIIYIYNNFIEYQDQSNENSKDLVLSLLIGLSDESDSINKKLLQFWDNNSKTLSASSNQRLQQFFDIMYSPETESKWVANSCCLLFQLCNRSSDFTKLLFDKPLSECTFKEVQVDSSWQHRTLNMNPLFSSSQYGIDDATTDESNGDSMQLDEIRATQAPNFTLTQTAFSEFYPSSSQSYGGTNNNTGSSQLSSSSSSSGSQSSSQNNSSSKRKQKITNDPKLINNSELRRRFKKIDDRGADERIVKFARLQVKRNIEREAYFEKSKQARENQITMIRKYRVGELPDIQIKLQDIIKPLQLLCQRDSTIGVNVFSSLFTALYKNTPKESIRTFQCNIKKLIDSIVERCKFNSTLVSSILNISEKNLEFSPEFSKIKDISLNSNNHPMAIILCEKLILSLQNTTKASTTNQAQKLQQQQQLNQGWDSLRELYKSLKEDDIIMGLIEKQMGGDIPYTKKALEFELKGDWVNVLKVYDEATSKLESGELNQYQGNLSESETALWENGRLECFTKLCNWSALKDNFNSYYPNPNQIFKEKNCDLLLSYFFEFNLKVKENWNYLYQFIADLANTKQYQYLENKFPGELAFLEVTRSDHNKASYYVSKFYQAFKHQWSSSHPLAIESRHRILQPLQKIVEVEEFLNLTSTPIINTLKLDTLLTQWKSRFPTKLDDIMVWDDLIFYRSVLLEKIYERFSSFTSDKSSDEKVKSTLIQERAILYHKMSKGARKLGNIVVSEIYFRQAVKSYPKTKDNDLAFPLVTSLIDIYCTKARNSPSPIETLDRFVKALKFIESKKDEESIINSNENLQKYLMMHGDIFWDIYQLDKKLGSTLVIDSFKKNSLPINLTSIPINSLKDELFNSTFKCYSESIKLHQKSTNLLNTTSSSPSLSISSSSSPYSSTSSSSQPKLSNEILIEKTKTKSAHLKFANFCDNILKDKISQQTTPTQFNEEVIDLATSVIVSTLEAIKEEIPGSVDKFPRLLEILTQFEQYSVIAREFKSRVSTIPCWMFIRWISQMFPYLDLPQGPLILPILLEIAKWYPQAIYFPFKISSEQFGPLAKKISAPLEKELINPLLDTLVIEFQRLTHPEHRFKDYMEEMKSLIKATPKDLNAIAKLNNEIYDDSFNPSTVSGDYNLKFAKEHEASYLSNFGKDSTKLARMDQKKFLEIFAEMSGNMNKNMKPNSTASMKLKDFSGWLADFDRSNYQTSHQMEIPGQYDGIGKPQPETHVTISSFDTNVLVMGSLRKPKRVKIHGNDELDYPFLIKGGEDLRLDQRIQQLFGIMNEILKRDTACNKRSLNVTTYQVVPMTSKVGIIEWLNDTKPLREILEEQLAHQLKTPRSNVSISKLESTKYHNDWINSFAKYLKPNSPVGPLYQQMFIHATRDDCAKKLEKQHSKVPENLLQNGIWSLSSSPESYLFIRNSFARSLASFSVCSYVIGIGDRHLENFLISQRDGRLIGIDFGHAFGTATQFLPIPELMPFRLTRQFTSFLRPLDSVGLLNHNMTYTLTALQNQKEILLTTMDVFVKEPLLDWSKLATRLVKEQGKHPKDTKNVWFPKQKIQIAKKKLELVNPAYITLEELSGSVHSGLPYEKALSEIIKGDPKHNIRAKVNKVCSSVKEQIDCLIDQSTDPNILSRAWVGWNGAL