Protein: Separase (EC 3.4.22.49) (Protein EXTRA SPINDLE POLES) (AtESP) (Protein RADIALLY SWOLLEN 4) (Q5IBC5)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Cleaves SYN1, releasing sister chromatid cohesion. Required for the release of cohesin at anaphase I and anaphase II, whereas the release of cohesin during diplotene and diakinesis occurs in a separase-independent process. Essential for embryo and endosperm development. May play a role in centromeric heterochromatin structure/formation during early meiosis, non-homologous centromere association and radial microtubule system (RMS) formation. May regulate the mitosis-specific cyclin CYCB1-1.
Interaction partners:
- Structural maintenance of chromosomes protein 3
- Mitotic checkpoint serine/threonine-protein kinase BUB1
- Structural maintenance of chromosomes protein 4
- Anaphase-promoting complex subunit 8
- Anaphase-promoting complex subunit 6
- Protein CHROMOSOME TRANSMISSION FIDELITY 7
- Mitotic spindle checkpoint protein BUBR1
- Sister chromatid cohesion 1 protein 1
- Anaphase-promoting complex subunit 10
- Mitotic spindle checkpoint protein MAD2
Sequence:
MASSGDDLRLLSLIDVGDNVFSSFSDYLKPFSTLSTSRKKQDRATTIRALAKQFLPFLNKSISLLPKRLSVANSDKEARESALDLFRAYELCLDCLELVSAQLACKPHTVQSQRLRMIHCLDVWGLYENVYTEAFKVLEKLRGSDSKSRKSRLLPEVQDGDAEMALVVVDAVAAIFRAVAMSQQLDDKRYRKVLLLLEEVGGWLRVLDAKVYEKLHRAMVTSMGKCAVSLVREAERFNGDLVISFCDLTVKEHYKSALSKDRVYKFAREVLSVLFGFKDRKMSVTIDISMSVLRSLSCQFEDESNENLMEFFDLVDYCAHKFRAAGDMYCAKVSKKLNEMAAIFVEAIPQLNLVLRLYSTGLSITVCNSKLGEIKLEDSTDDWKIQAMFDDDARWQSLVSLLGMVDSYSGDEGNQTGSSSIGGHRNYNNKTHDSCKDRNKITCWPQYVDALKFLCQPLADFIYSVKRKIVLETEMSCASAHLITIHDAFLQFCDGCLFLQRCTSDKGDREIANNKAFLNAAMGAFIVSLRTQLKLEISAHLVEDVIGSPWIQSQELKYLIATLYNIGIVLYRNKELNKACEALKLCSKVSWRCVELHCHMFVNQSSSSDNDLSEDAIMDFVGEACNRCAFYLDILQKCSRRKIRQNIVHILENWLSAEHLIRRLPGPEAIVKQWVKIERECHTDLDAAGSCTTLYSLLSSSQKKSKRGIGKILEQELLAYDRVLPLRSNLGQQTRIKIADILLKDVYVTEDMHIERARILIWKARMTRTSGTEHITECICFLSEAISILGELHHGPNEEGSPSSHMLPIAYCLRAFCTQEADPNSKKVFQDISTSLNLWLRILSLDDSGDSLPTENIIPLLYNMIDLMSVKGCTELHHHIYQLIFRLFKWKNVKLEVCLAMLWECRRLSHALCPSPISDAFIQTLSENCADKSTCIDFWMDCLKDSKAKLIGFQQNFHDLHNKDEGPFQSDITIDDIKDAASELISSASLSGNSSFAAAYLYYDLCERLISFGKLSEALSYAKEAYRIRTLIFQDKFKYTAEKHIEKHNEDGKISEIRTFSIKNFQVYRLLATDFWPCGNFLWDINRCYLSPWSVLQCYLESTLQVGILNELIGNGLEAETILSWGKAFSCSQSLFPFVVAFSSALGNLYHKKQCLDLAEKELQNAKEILIANQRDFSCVKCKLKLEVTLDKQLGDISRKQIDRVSQTDGFLHAESLFSAALGKFCCSAWKSCIRSHGEEIAEEIVIDRNGGEGLGHNSSKTKLSIKEPPGNRGSRRGGRANKTCLSKDQDLISEPTSRLTRSMRHSLREQCQNRSNVPEVVSKKPNLCDRSVGSRGERVLLDTSNALPGFCICYKEKRQQCLSEEVTESGSLNNLVSLKWELCHRKLASSILVSLGKCLGDSGRIHLAHEALLHSISVLFKSTWSSHNQPSVSQLLEFIGKEVTRDVFAVDRAIILYNLCWLNLRNYHCRKSRSICCDLFHIPFTKLVSWLMLAFVLSGEVPILFQKVSRLLASLYLLSSSNSEFTFESDGNELSASHWVSFFHQASLGTHLSYHFISNLSQKHKSQCLSDKECTEATCSSCMVPEDLDLPRLAPDRTQDLVQFAKEFFINLPSSTIICISLLGGALNQLLQELMHIRSPVCAWVLISRLNPESQPVATLLPVDSIVEDMSDDSANLSSTEATQVKSLKGPWLCPWGTTVVDEVAPAFKSILEESHSSSSTTEEDTIESRGLWWKKRKKLNHRLGIFLRNLEASWLGPWRCLLLGEWSNYKLPDSAQKKLVNDLKSKCKMEVNEMLLKVILGGGTDNFKGEACVAQLSLRNGCYVGRGGYLYEEDSCKTPTAASNISESRHELALKLIHDAASKLGQQDGHENREPIILVLDPEVQMLPWENIPILRKQEVYRMPSVGCISAVLKKRSLQGEPAKSHVASFPLIDPLDSFYLLNPGGDLTDTQVTFESWFRDQNFEGKAGSEPSAIELTEALETHDLFLYFGHGSGAQYIPRREIEKLDNCSATFLMGCSSGSLWLKGCYIPQGVPLSYLLGGSPAIVATLWDVTDRDIDRFGKALLEAWLQERSDSSSEGGCSQCESLANDLAAMTLKGTKRSRKPSSRNKPAQSDVDGSGKIECNHKHRRKIGSFIAAARDACNLQYLIGAAPVCYGVPTGITRKKGIDALLPSSSR