Protein: Ethylene-insensitive protein 2 (AtEIN2) (EIN-2) (Cytokinin-resistant protein AtCKR1) (Protein ORESARA 3) [Cleaved into: EIN2-CEND (EIN2C)] (Q9S814)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Central factor in signaling pathways regulated by ethylene (ET) and involved in various processes including development, plant defense, senescence, nucleotide sugar flux, and tropisms. Necessary for ethylene-mediated gene regulation, and for the induction of some genes by ozone. Acts downstream of ET receptors, and upstream of ethylene regulated transcription factors. Required for cytokinin-mediated processes. Seems to be implicated in cross-talk between ET, jasmonate and other pathways. Probably not involved in iron uptake. Has a short half-life and undergoes rapid proteasome-mediated turnover in the absence of ethylene. Required for ethylene-induced EIN3 stabilization via proteasomal degradation of EBF1/EBF2 proteins. Regulates the leaf senescence induced by methyl jasmonate, ethylene and abscisic acid. Required during salt stress to confer resistance.
Subcellular location: [Ethylene-insensitive protein 2]: Endoplasmic reticulum membrane {ECO:0000269|PubMed:19769567, ECO:0000269|PubMed:22936567, ECO:0000269|PubMed:23070300, ECO:0000269|PubMed:23132950}; Multi-pass membrane protein {ECO:0000255}.; SUBCELLULAR LOCATION: [EIN2-CEND]: Nucleus {ECO:0000269|PubMed:22936567, ECO:0000269|PubMed:23070300, ECO:0000269|PubMed:23132950, ECO:0000269|PubMed:25822663, ECO:0000269|PubMed:26496607, ECO:0000269|PubMed:26496608, ECO:0000269|PubMed:26507893}. Cytoplasm {ECO:0000269|PubMed:23070300, ECO:0000269|PubMed:25843012, ECO:0000269|PubMed:26496607, ECO:0000269|PubMed:26496608}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:25822663, ECO:0000269|PubMed:25843012}. Note=Perception of ethylene or methyl jasmonate leads to proteolytic cleavage allowing the C-terminal domain to localize to the nucleus while the N-terminus remains at the endoplasmic reticulum membrane. Apart from nuclear localization, some association with endoplasmic membranes and some speckles are also observed in the cytoplasm. During hypoxia (e.g. submergences), translocates from endoplasmic reticulum (ER) to the nucleus via a ceramides-triggered and CTR1-dependent manner (PubMed:25822663). {ECO:0000269|PubMed:22936567, ECO:0000269|PubMed:23070300, ECO:0000269|PubMed:23132950, ECO:0000269|PubMed:25822663, ECO:0000269|PubMed:25843012, ECO:0000269|PubMed:26496607, ECO:0000269|PubMed:26496608, ECO:0000269|PubMed:26507893}.
Interaction partners:
- Protein ETHYLENE INSENSITIVE 3
- ETHYLENE INSENSITIVE 3-like 1 protein
- Ethylene receptor 1
- Serine/threonine-protein kinase CTR1
- ETHYLENE INSENSITIVE 3-like 2 protein
- ETHYLENE INSENSITIVE 3-like 3 protein
- EIN3-binding F-box protein 1
- Putative ETHYLENE INSENSITIVE 3-like 4 protein
- Ethylene response sensor 1
- Protein EIN4
Sequence:
MEAEIVNVRPQLGFIQRMVPALLPVLLVSVGYIDPGKWVANIEGGARFGYDLVAITLLFNFAAILCQYVAARISVVTGKHLAQICNEEYDKWTCMFLGIQAEFSAILLDLTMVVGVAHALNLLFGVELSTGVFLAAMDAFLFPVFASFLENGMANTVSIYSAGLVLLLYVSGVLLSQSEIPLSMNGVLTRLNGESAFALMGLLGASIVPHNFYIHSYFAGESTSSSDVDKSSLCQDHLFAIFGVFSGLSLVNYVLMNAAANVFHSTGLVVLTFHDALSLMEQVFMSPLIPVVFLMLLFFSSQITALAWAFGGEVVLHDFLKIEIPAWLHRATIRILAVAPALYCVWTSGADGIYQLLIFTQVLVAMMLPCSVIPLFRIASSRQIMGVHKIPQVGEFLALTTFLGFLGLNVVFVVEMVFGSSDWAGGLRWNTVMGTSIQYTTLLVSSCASLCLILWLAATPLKSASNRAEAQIWNMDAQNALSYPSVQEEEIERTETRRNEDESIVRLESRVKDQLDTTSVTSSVYDLPENILMTDQEIRSSPPEERELDVKYSTSQVSSLKEDSDVKEQSVLQSTVVNEVSDKDLIVETKMAKIEPMSPVEKIVSMENNSKFIEKDVEGVSWETEEATKAAPTSNFTVGSDGPPSFRSLSGEGGSGTGSLSRLQGLGRAARRHLSAILDEFWGHLYDFHGQLVAEARAKKLDQLFGTDQKSASSMKADSFGKDISSGYCMSPTAKGMDSQMTSSLYDSLKQQRTPGSIDSLYGLQRGSSPSPLVNRMQMLGAYGNTTNNNNAYELSERRYSSLRAPSSSEGWEHQQPATVHGYQMKSYVDNLAKERLEALQSRGEIPTSRSMALGTLSYTQQLALALKQKSQNGLTPGPAPGFENFAGSRSISRQSERSYYGVPSSGNTDTVGAAVANEKKYSSMPDISGLSMSARNMHLPNNKSGYWDPSSGGGGYGASYGRLSNESSLYSNLGSRVGVPSTYDDISQSRGGYRDAYSLPQSATTGTGSLWSRQPFEQFGVAERNGAVGEELRNRSNPINIDNNASSNVDAEAKLLQSFRHCILKLIKLEGSEWLFGQSDGVDEELIDRVAAREKFIYEAEAREINQVGHMGEPLISSVPNCGDGCVWRADLIVSFGVWCIHRVLDLSLMESRPELWGKYTYVLNRLQGVIDPAFSKLRTPMTPCFCLQIPASHQRASPTSANGMLPPAAKPAKGKCTTAVTLLDLIKDVEMAISCRKGRTGTAAGDVAFPKGKENLASVLKRYKRRLSNKPVGMNQDGPGSRKNVTAYGSLG