Protein: ATP-dependent RNA helicase A (EC 3.6.4.13) (Nuclear DNA helicase II) (NDH II) (Q22307)
Organism: Caenorhabditis elegans
Function: Multifunctional ATP-dependent nucleic acid helicase that unwinds DNA and RNA in a 3' to 5' direction and that plays important roles in many processes, such as DNA replication, transcriptional activation, post-transcriptional RNA regulation, mRNA translation and RNA-mediated gene silencing. Requires a 3'-single-stranded tail as entry site for acid nuclei unwinding activities as well as the binding and hydrolyzing of any of the four ribo- or deoxyribo-nucleotide triphosphates (NTPs). Binds to DNA, RNA and small interfering siRNA. Plays a role in DNA replication at origins of replication and cell cycle progression (By similarity). Plays a role as a transcriptional coactivator acting as a bridging factor between polymerase II holoenzyme and transcription factors or cofactors. Plays several roles in post-transcriptional regulation of gene expression. Promotes pre-mRNA alternative splicing activities of a subset of genes. As component of a large PER complex, is involved in the negative regulation of 3' transcriptional termination of circadian target genes. Component of the coding region determinant (CRD)-mediated complex that promotes cytoplasmic MYC mRNA stability. Plays a role in mRNA translation. Also plays a role in the RNA-induced silencing complex (RISC) loading complex (RLC) assembly, and hence functions in the RISC-mediated gene silencing process. Mediates the attachment of heterogeneous nuclear ribonucleoproteins (hnRNPs) to actin filaments in the nucleus (By similarity).
Subcellular location: Nucleus {ECO:0000250|UniProtKB:Q08211}. Nucleus, nucleoplasm {ECO:0000250|UniProtKB:Q08211}. Nucleus, nucleolus {ECO:0000250|UniProtKB:Q08211}. Cytoplasm {ECO:0000250|UniProtKB:Q08211}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:Q08211}. Note=Nucleoplasmic shuttling protein. Its nuclear import involves the nucleocytoplasmic transport receptor Importin alpha/Importin beta receptor pathway in a Ran-dependent manner. In interphase, localizes in nuclear stress granules and at perichromatin fibrils and in cytoplasmic ribonucleoprotein granules. Excluded from the mitotic nucleus as early as prophase and re-entered the nucleus at telophase. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. {ECO:0000250|UniProtKB:Q08211}.
Interaction partners:
- DeHydrogenases, Short chain
- Nucleolar protein 6
- Small-subunit processome
- Dehydrogenase/reductase SDR family member 1
- SPK domain-containing protein
- DNA methyltransferase 1-associated protein 1
- Tar DNA-binding protein homolog 1
- Chromatin-remodeling complex ATPase chain isw-1
Sequence:
MSRDVKEFLYAWLGKNKYGNPTYDTKSETRSGRQRFKCELRITGFGYTAFGNSTNKKDAATNAAQDFCQYLVREGKMQQSDIPTLTSSSLEASSTWQDSETATMFCGGEDGNSFQESQQPIPQKRFPWSNNAYQRNEGTHEQYITQKAEEIAASETVDLKSEIHGGWTMENSKKALNEFLQKMRLPQVNYGTKIRESNTVKTMETTAQIFVPQINKNLVGKGTGSNKKVSEAACAMNIVRQMFHLNIMQSYSGPIKKSKVSTLPEIAISIPEDLSTRVTEYVRSCGLELPEINETSATPEAPTSLLTDVKLAQFPVSEICSASNISWAPPLQNWNPWRASNIDEEPLAFMSMEQISQRIMEKEDFKRGEALDKITAQRGELPVAQYRENIVQTVAENRVTLIKGETGCGKSTQVAQFLLESFLENSNGASFNAVVSQPRRISAISLAERVANERGEEVGETCGYNVRFDSATPRPYGSIMFCTVGVLLRMMENGLRGISHVIIDEIHERDVDTDFVLIVLREMISTYRDLRVVLMSATIDTDLFTNFFSSIPDVGPTPVITMHGRTFPVQSFYLEDILHNLQHMPEEPDQKKRKKGGPPPPDDDEGDEEVDDKGRNMNILTDPSINESLKTAMSRISEKDIPFGVIEAILNDIASRGVDGAVLVFLPGWAEIMTLCNRLLEHQEFGQANKYEILPLHSQLTSQEQRKVFNHYPGKRKIIVSTNIAETSITIDDVVYVIDSCKAKERMYTSNNNMVHFATVWASKTNVIQRRGRAGRVRAGYAFHLCSKMRFEALDDHGTAEMLRIPLHQIALTIKLLRLGSVGEFLGKALQPPPYDMVVESEAVLQAMGALDRNLELTSLGKMLARMPIEPVIAKVLILGTALGAGSVMCDVASAMSFPTPFVPREKHHSRLSGTQRKFAGNKFSDHVAIVSVIQGYREAVQMGASAAEREFCERYSLSNPVLKMTDGARRQLIDVLRNQCSFPEDILFDISVNVNGPDRELNLMRSLLVMALYPNVAYYVGKRKVLTIEQSSALINKYSMLVPMNNRQEMDFPSPLLVFTEKVRTRCISCKQMSVISAIQLLVFGSRKVECVGEGLVRIDETITIRMNVSTAAALIGLRPCIEALLVKSCENPESLAGLNPSDAELRQLLRDISSEEFMSEAGPIKDSLLTDNALIQKGPAPNRLEYADWGPSNNNSSFQNQDFPPAAGGKVHPYRGNRRGNHPYAQNRPYAPPNSGMGYQNFNNSGYGASGDWNAGRGNYGNVGSGYRGAGGGGGYRGGRGGRGGGRGGGGRGWNASNW