Protein: Rho GTPase-activating protein Graf (GTPase regulator associated with focal adhesion kinase) (X2JDY8)
Organism: Drosophila melanogaster (Fruit fly)
Function: GTPase-activating protein for Rho family proteins. Essential component of the CLIC (clathrin-independent carrier)/GEEC (GPI-anchored protein-enriched early endocytic compartment) endocytic pathway. During hematopoiesis, inhibits Egfr-ras-MAPK signaling by promoting Spi-induced Egfr internalization through CLIC/GEEC endocytosis, thereby preventing plasmatocyte overproliferation. Essential for normal mushroom body (MB) development and consequently the formation of olfactory long-term memories. During MD development, required to stop the MB beta-lobe from crossing the brain midline, possibly acting via its role in the CLIC/GEEC endocytic pathway to down-regulate the Egfr-ras-MAPK signaling at the tip of the beta-lobes. Required during embryo cellularization for maintaining and regulating the rate of actomyosin ring constriction. During cellularization, inhibits Rho-GTP levels at the furrow canal tip in a spatiotemporal manner, thus delaying the onset of actomyosin contraction and ensuring appropriate closure of the cells at the base of nuclei after membrane extension.
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:33835025}. Cytoplasm {ECO:0000269|PubMed:28993397}. Cytoplasmic vesicle {ECO:0000269|PubMed:28993397}. Note=During embryonic cellularization, detected at the furrow canal tips with enrichment at the edges (PubMed:33835025). Recruited to the furrow tips at early cellularization, expression levels are highest during mid-cellularization but then decrease at late cellularization when it becomes predominately cytosolic (PubMed:33835025). In embryos and larvae, displays a punctate localization in the cytoplasm of plasmatocytes (PubMed:28993397). In larvae, also displays a punctate localization in hemolymph crystal cells (PubMed:28993397). During Egfr internalization, localizes with Egfr and spi, to punctate or tubular structures connected to the cell surface (PubMed:28993397). {ECO:0000269|PubMed:28993397, ECO:0000269|PubMed:33835025}.
Interaction partners:
Sequence:
MGGGKNVRRGLEPLEFEECIVDSPDFRENLNRHEKELDHTSHQIKRIIKEVKDLMSAAKILSTRMKQLAILLNDFNFECIGTAQTDDENVICESLKRFGAIIGNIEDEREKMLTLADKHIIESLEDFRKKQIGGVKENKKKFDKKTEKFCQSQERFLNMSTKKPENTIQEADASLGMHEREYIQESLSYVLRIQEVQERIKFEFVEILLAFISGWLVFYHTAHEQAEDHRDYLQDLRHKVQKTRENFEEAREKVTELKTKYMEKRTKPEEIFTKRGYLFLMEKSSILKISLLEPFKATWTKYYCTFKKQKREFTMLQFNQMNHNFTRPEARDDEKLTLFSCQRRASEFEKRFCFDLTFKEKPGVVYTFQALSEKDHRYWISAMDGTEPTYLAPGKIKVSEAYHLDEAGFMFIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKESDDVFVDDKYRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQETLNQRVNEVHKLVYKLPQPNFQMLDMVICHLTDVSRKYEKNKMSVFNLGVVFGPTLLRPREESVAAILDIKFNNIVINILIDNYERIFKNKPSADIKLPDATKAPSIMYPSRSSPPTRMPRASQIGKAASTGAMGSSSNTAGNPMFLNQQKIYRVVSKTNCTEPTMSSSLQNIPNGDNYALGSNAMNSSGGAQCISLSPPMHMLNGILSPTIGSINNLHTISKNETSTRRYVPDTEIAPDYGIIGANNGGVTLQSHHAVPVSSTSNFRHPEYLMTTANPVTQSGSSSHIYTNTSSAGANSSNRISLSNVSPPNTAMRKERFLGSASGPQQHPPVQRGLHSYGQTKHYSPLMPTSTSSSNDSVCDSLSSNNGLGSSIVANSGNSGNVAQHLSARNTNDYALISSQNSSLSPHLGPTCDEVVTATTLPSVSLSCGGTASESTEYPPSKMHRNRDVNQIKRDLSTGTARVRTLYACMGESEGELSFEPNQIITNVRYSHEPGWLQGTLNGKTGLIPENYVEHLKPHH