Protein: Kinetochore-associated protein rod-1 (Rough deal homolog) (Q20849)
Organism: Caenorhabditis elegans
Function: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for chromosome segregation, the assembly of the dynein-dynactin and mdf-1-mdf-2 complexes onto kinetochores and spindle pole separation. Plays a role in nuclear envelope breakdown. Its function related to the spindle assembly machinery and kinetochore-microtubule attachments likely depends on its association in the mitotic RZZ complex. The RZZ complex recruits the spindly-like protein spdl-1 to kinetochores. To prevent irregular chromosome segregation, the complex also inhibits the attachment of the kinetochore-associated NDC80 complex to microtubules. The recruitment of spdl-1 to kinetochores relieves this inhibition. Required for embryonic development.
Subcellular location: Chromosome, centromere, kinetochore {ECO:0000305|PubMed:18765790}. Cytoplasm, cytoskeleton, spindle {ECO:0000250|UniProtKB:P50748}. Note=Localizes to the kinetochore during nuclear envelope breakdown and remains there until the metaphase-anaphase transition. Localization of the RZZ complex to kinetochores is dependent upon knl-1. {ECO:0000305|PubMed:18765790}.
Interaction partners:
- Protein zwilch homolog
- Centromere/kinetochore protein zw10 homolog
- Calponin-homology
- Kinesin-like protein
- Mitotic chromosome and X-chromosome-associated protein mix-1
- Mitotic spindle assembly checkpoint protein MAD2A
- HoloCentric chromosome binding Protein
- FANCD2
- BUB1 N-terminal domain-containing protein
- Nuclear Pore complex Protein
Sequence:
MGRLGEAKAVDLEAETSTVGLYDVCTVGRILPLLNEMSIDKKSPAWKLKPKSNERFLVLASCSDLAVFSLGEKSDYSFNTGLGGAITDFEILGSSSFFVGVIEHRRIVILSLDKQEIYLNEPAPHEIDFVVCHTTSSVCQLIFGSRSDNWHTITLPGDVAASKETNRFENWHRDQIQEFFHQAMGKTVSKSVDMSKVAGDITLISNGPLQTFASIDQQRGIHWCGLVESEFEVIGMEKQFLQVRDGGRFSLHLDTDGWIHVFDSLVSSFCHEFDMKMSPDDKILDFVIIDKNEVEVPKMFAILVLPRDGDSTKMMIYDRLNKDNCFALDSSTSTTLFAYGGSDRVLIAVEELENSLADEQDGSQIVVRQVSQSRPEMRFESLLKNNRFEEAEKFAMAFMLDVQKVYKGHVHYLMDSCDESDESFETLMTKMGQIQDHNMVAETYFTLVGMSRRCDRIRTYLTHAKKRRITDLDILKMIEALCYTWGTYRIIAGPEENEPSRPQVNETIWDLFVEALHDANPWIEIYNEFISDAQFTQARVIFSRHGKSITDYMCDDEENTISRLETLFRMFIDAISSDISKWSNVIEHVTTDILPACVMITEELIPCLESLITSLISLLEYRDSTNWPENAIKAASSYDTMTKILSNNGNTPATQCILVMYGSKLGSSAGNKPSSMSRIKKIYYDLIELKRLKDVYECSISFSVFQNMSSEQICHKILQNALANPNMTHAKIEKFVKPFMAERHLDQEQTIVNYIQMMSGAAVTNANLFGWEKQCVQLCASLMDETRRCCSIISIASTAKIPWPAELNEAVEKILASRTLLRSEIEQMHLVCKRTELYKMLSSYGFSRQDIELLTTPDSNMDIILTIRCMLAHREKASRFVDVIKLVDLLKAMQGSSQPRTLRIEYVQSFAIIHMMSHQDVTISIINYIDSLGDRERVKTISLVFSFIECVANAPATGDNVLEREKILGVGEELMSHYTCRDNNFNDPERRLKDELVLLREVQKTETKAVLLSELKDEDWQRRFLERLIESNSSMSLNLGRCSYMGISPEHLIEMILTKATVENDMDTIVDSITNYVEFINSLTDSSREMLEPIVQILSWITFRLPKLLPNETEMARADYIAFVVKRIGRVVRETLRRFSFDVISDDLDYLLQLEAFYHLGEHIIKQSLRGQENENEKQEMFRSDDDTFLPTDSSNPFSNQSSLRIFEFKRPLGTFDFSNDPALFEGVQGVLALAMVAPSVARPYDSEISPDDANEFRSSWEQLNMFLAMHSQDLLDISARVFAGSLKCWAGEYLQGIVEMEQPILSVVERMLQQKKFDFWHAVTLLGGIPLERLDRAIIDLQKRQGVRSSTKATIQYLQLAFVMSLLARNMEKVPTIVSAYEQKYLVKKLAEEGIRVSITQDFVDKVLQQAIDLRQPLSPLRLHDYVKKYVEKLIRNSKISVGEYMVRYATLLIRKASVAGRHTDREIRKEEIEKYIEAARIALRIAEEEDASCICNYLHCLLYVVCPYNYEVIQFIVTSYGKYATETVEIEFNKNLKSIMAFLWAYQRTNHISNEESIWFTKRESVLMKDEKEFEKTGRMLDPFGHSLRVVYEDDGSNMSDSYNSDLALSSDAMVYERNSVIISDLPSLAEQYLPFHAFLLRKKEEIDEIVMGIVKAELSIFNVPIWQTFLREVSWLSSRFSRSQLLSSAIFAHANKYAKFGKSLPDGERNVIYELLNSASQRHVVVSTIALLFKRIILSDVKIELLQMGVNISERWTSDLTGEEQQEMEEQSARLKDGIAKFSTELELKKNGLYNEKTADNIENVSELCSLIYNEMVQWDDSRDVLKKCQVVDKIAKANGLDLTALHEQLVFSWVEDTQTIISINHVDMNESIGGTSFLDHKDETDDQNDLRIPLFDGILDKVVVLCQRIDKKRLLTRLGSILMRGGRKATGGYTAVVRATCIILRSFTDTEVSELLSGADMFALCSTLENQLYERLFEKAEVKGDCKTDKMQLIKSLLQCPSRTHPMTALIACLIIDHEFKDPKRDISLGWDVGCVPVSANSSNS