Protein: Autophagy-related protein 16-1 (APG16-like 1) (Q8C0J2)
Organism: Mus musculus (Mouse)
Function: Plays an essential role in both canonical and non-canonical autophagy: interacts with ATG12-ATG5 to mediate the lipidation to ATG8 family proteins (MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP). Acts as a molecular hub, coordinating autophagy pathways via distinct domains that support either canonical or non-canonical signaling. During canonical autophagy, interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to ATG8 proteins, to produce a membrane-bound activated form of ATG8 (By similarity). Thereby, controls the elongation of the nascent autophagosomal membrane (By similarity). Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, probably by catalyzing conjugation of phosphatidylserine (PS) to ATG8 (By similarity). Non-canonical autophagy plays a key role in epithelial cells to limit lethal infection by influenza A (IAV) virus. Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (By similarity). Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response.Instead, promotes with NOD2 an autophagy-dependent antibacterial pathway. Plays a role in regulating morphology and function of Paneth cell (By similarity).
Subcellular location: Cytoplasm {ECO:0000269|PubMed:33087875}. Preautophagosomal structure membrane {ECO:0000269|PubMed:24954904}; Peripheral membrane protein {ECO:0000305}. Endosome membrane {ECO:0000250|UniProtKB:Q676U5}; Peripheral membrane protein {ECO:0000305}. Lysosome membrane {ECO:0000250|UniProtKB:Q676U5}; Peripheral membrane protein {ECO:0000305}. Note=Recruited to omegasomes membranes by WIPI2 (PubMed:24954904). Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5 (PubMed:24954904). Localizes also to discrete punctae along the ciliary axoneme (PubMed:24954904). Upon activation of non-canonical autophagy, recruited to single-membrane endolysosomal compartments (By similarity). {ECO:0000250|UniProtKB:Q676U5, ECO:0000269|PubMed:24954904}.
Interaction partners:
- Ras-related protein Rab-33B
- Nucleotide-binding oligomerization domain-containing protein 2
- WD repeat domain phosphoinositide-interacting protein 2
- Nucleotide-binding oligomerization domain-containing protein 1
- Ubiquitin-like-conjugating enzyme ATG3
- Ubiquitin-like protein ATG12
- RB1-inducible coiled-coil protein 1
- Autophagy protein 5
- WD repeat domain phosphoinositide-interacting protein 1
- Ubiquitin-like modifier-activating enzyme ATG7
Sequence:
MSSGLRAADFPRWKRHIAEELRRRDRLQRQAFEEIILQYTKLLEKSDLHSVLTQKLQAEKHDMPNRHEISPGHDGAWNDSQLQEMAQLRIKHQEELTELHKKRGELAQLVIDLNNQMQQKDKEIQMNEAKISEYLQTISDLETNCLDLRTKLQDLEVANQTLKDEYDALQITFTALEEKLRKTTEENQELVTRWMAEKAQEANRLNAENEKDSRRRQARLQKELAEAAKEPLPVEQDDDIEVIVDETSDHTEETSPVRAVSRAATKRLSQPAGGLLDSITNIFGRRSVSSIPVPQDIMDTHPASGKDVRVPTTASYVFDAHDGEVNAVQFSPGSRLLATGGMDRRVKLWEAFGDKCEFKGSLSGSNAGITSIEFDSAGAYLLAASNDFASRIWTVDDYRLRHTLTGHSGKVLSAKFLLDNARIVSGSHDRTLKLWDLRSKVCIKTVFAGSSCNDIVCTEQCVMSGHFDKKIRFWDIRSESVVREMELLGKITALDLNPERTELLSCSRDDLLKVIDLRTNAVKQTFSAPGFKCGSDWTRVVFSPDGSYVAAGSAEGSLYVWSVLTGKVEKVLSKQHSSSINAVAWAPSGLHVVSVDKGSRAVLWAQP