Protein: Phospholipase D alpha 1 (AtPLDalpha1) (PLD alpha 1) (EC 3.1.4.4) (Choline phosphatase 1) (PLDalpha) (Phosphatidylcholine-hydrolyzing phospholipase D 1) (Q38882)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond to generate phosphatidic acids (PA). Plays an important role in various cellular processes, including phytohormone action and response to stress, characterized by acidification of the cell. Involved in wound induction of jasmonic acid. May be involved in membrane lipid remodeling. Probably involved in freezing tolerance by modulating the cold-responsive genes and accumulation of osmolytes. Can use phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) as substrates, both in presence or in absence of PIP2. Its main substrate is phosphatidylcholine. Stimulates the intrinsic GTPase activity of GPA1 upon binding. Mediates the abscisic acid effects on stomata through interaction with GPA1 and the production of phosphatidic acid that bind to ABI1. Involved in seed aging and deterioration. Involved in microtubule stabilization and salt tolerance. Involved in abscisic acid-induced stomatal closure.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:10198096, ECO:0000269|PubMed:11706190}. Cell membrane {ECO:0000269|PubMed:10198096, ECO:0000269|PubMed:11706190}; Peripheral membrane protein {ECO:0000269|PubMed:10198096}. Mitochondrion membrane {ECO:0000269|PubMed:11706190}. Microsome membrane {ECO:0000269|PubMed:11706190}. Vacuole {ECO:0000269|PubMed:10198096}. Cytoplasmic vesicle, clathrin-coated vesicle {ECO:0000269|PubMed:10198096}. Note=Not found in chloroplast or nuclei. The distribution of this conventional PLD between membrane-associated and soluble fractions varied from organ to organ and is calcium-regulated. Activation or wounding increases association of preexisting enzyme with membranes. {ECO:0000269|PubMed:11090221}.
Interaction partners:
- Guanine nucleotide-binding protein alpha-1 subunit
- Non-specific phospholipase C4
- Non-specific phospholipase C5
- Non-specific phospholipase C1
- CDP-diacylglycerol--serine O-phosphatidyltransferase 1
- Non-specific phospholipase C6
- Non-specific phospholipase C2
- Acyl-CoA-binding domain-containing protein 1
- Phosphatidylserine decarboxylase proenzyme 2
- Phosphatidyl-N-methylethanolamine N-methyltransferase
Sequence:
MAQHLLHGTLHATIYEVDALHGGGVRQGFLGKILANVEETIGVGKGETQLYATIDLQKARVGRTRKIKNEPKNPKWYESFHIYCAHLASDIIFTVKDDNPIGATLIGRAYIPVDQVINGEEVDQWVEILDNDRNPIQGGSKIHVKLQYFHVEEDRNWNMGIKSAKFPGVPYTFFSQRQGCKVSLYQDAHIPDNFVPRIPLAGGKNYEPQRCWEDIFDAISNAKHLIYITGWSVYAEIALVRDSRRPKPGGDVTIGELLKKKASEGVRVLLLVWDDRTSVDVLKKDGLMATHDEETENFFRGSDVHCILCPRNPDDGGSIVQSLQISTMFTHHQKIVVVDSEMPSRGGSEMRRIVSFVGGIDLCDGRYDTPFHSLFRTLDTVHHDDFHQPNFTGAAITKGGPREPWHDIHSRLEGPIAWDVMYNFEQRWSKQGGKDILVKLRDLSDIIITPSPVMFQEDHDVWNVQLFRSIDGGAAAGFPESPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYVENQYFLGSSFAWAADGITPEDINALHLIPKELSLKIVSKIEKGEKFRVYVVVPMWPEGLPESGSVQAILDWQRRTMEMMYKDVIQALRAQGLEEDPRNYLTFFCLGNREVKKDGEYEPAEKPDPDTDYMRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPHHLSHRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSLECIEKVNRISDKYWDFYSSESLEHDLPGHLLRYPIGVASEGDITELPGFEFFPDTKARILGTKSDYLPPILTT