Protein: E3 ubiquitin-protein ligase lubel (EC 2.3.2.31) (Linear Ubiquitin E3 ligase) (Q8IPJ3)
Organism: Drosophila melanogaster (Fruit fly)
Function: E3 ubiquitin-protein ligase which conjugates linear 'Met-1'- and 'Lys-63'-linked polyubiquitin chains to substrates and plays a crucial role in the NF-kappa-B intestinal inflammatory response to oral infection and in the heat stress response. Preferentially interacts with 'Lys-63'-linked, and to a lesser extent 'Lys-48'-linked, polyubiquitin chains. Upon oral infection with a Gram-negative bacterium E.carotovora subsp. carotovora 15, functions with the E2 ubiquitin-conjugating enzyme Ubc10 to mediate the conjugation of 'Lys-63'- and linear 'Met-1'-linked polyubiquitin chains to the substrate key which is essential for activation of the NF-kappa-B signaling cascade in the adult intestinal epithelium. It is not required for systemic immune response to septic infection with either E.carotovora subsp. carotovora 15 or Gram-positive M.luteus bacteria. Function in controlling linear ubiquitination is also essential for regulating the heat stress response in adults. This function may require the E2 ubiquitin-conjugating enzymes Ubc10 or eff.
Interaction partners:
- ubiquitinyl hydrolase 1
- Ubiquitin-ribosomal protein eS31 fusion protein
- FI05319p
- Ubiquitin-conjugating enzyme E2-17 kDa
- Polyubiquitin
- Uncharacterized protein, isoform C
- Ubiquitin-ribosomal protein eL40 fusion protein
- NHP2-like protein 1 homolog
- GH17761p
- Ubiquitin-like domain-containing protein
Sequence:
MTTHQLLNKNVRTMPSWVMEANDRIGPKPPPTPPNGVAGGLPKAPALPPKAKSTPEPDYEIIEFSSQQYSNEPMKTTVIRTKTPDNKLKCTLCGSQNPWVTCAECAGQIFCASCDDMFHKHPKRKQHMRKAVEQGTPPIPPKAQAGGGAPPPVAPPRRSKRGLLTPFLGRKDQMLPPPSPTPSHKSLGGWRGSLGGGATPPVPPIATSSANQMNNRPLPDPPRSEGGGSSRSGTPKSVFDTIQRPPSVQLEKIKSKASATLDRMAILQQRYRQQKARQDLSANSEQHLSNEAGFEHWSNISPSPSHFRSGSMSSGLNSSHFDLSDDSQFHNSLLLQQRQAAGAQRRQMSTSVFNLNSNPRRPLSEAQNGGAWLANQRIQQAQSLAQLNCAGCQQSQQHPGWAQHPHQALPQHQHPDQWSQFGSQQQFNNSNLSLNVGPGYMSQQHHPHYPPPVFMTQRGMMPNVYPGAPGYPMMHPGVMGMPPSAASRAASRSRYAASPTPSRKSMSLRRKRNSYVDDELTDDEDSDQDDRRSLVSNRSGMTSASRSQHHQNHIQPRQRRLSSASQLIASDELDGDQVHHKMRNRRGSIAKSVQSEWLPERRENEGTLTRNKTATDSARTSRIYSDLESEGSGARALVQAKIQQKLQEADQHKSSKKAEPKRKPEMKDENTQAAAVVQKVVVPPAHEESASEYEEVVEEVTASESEAEAQTAPDPQEVPDEIGADDLGPPPSTPDHEWECEFCTFVNEPNIKICSICCKTPSKPPVQPNKAKKVEEKPQPPAEKSNNIKASSKTETKPVQKPTTKSQQPSQKSVAALSKTTHTNSTSSSKASPAVNSKTTSSIPIKTPSKSTLKTSSENESDNSLAKSLLHKESVENIWNTLDESIQAQAEQVLKKAQKVSTACGGTPPREIAAVEMGTSPPPQSISTQTYDALPFNTKQEEIIPVVPDRFTTPEPNKMERRPHYRSNSQLQQESERYRSANDLRYHDGFGLDPYSAGLVTKRPNFINELRMLQLQVSSPFDMPHETFGVKHEPARDPETEMHIILKELELYKFTVEELEAALKYCSPETHPIQWLRENWHKLVQTVQSLSTKYGQERGENTIGTVSQNEAREALRNSGGNVWQAVADCIQQRQQKYRKLAAKGNFLRDDIVNALTAHQGNVEQALVELNRTQLKPFLMRIWGSPNGVENESGVAIDTKSDIHDFLNTHALDCLQPPLAGQSPSPAQANPFDQPRTDESPVKSTYATPSPYQMEDSTLKNLEILIGNMEQNQAKQNQEVLRSIETMLDTFKGKPELEYETDPEIMRILTKSPISTMKPSGPAEDKSTDDVKNFVWQHIQEIVPNLVQQVEQELMEKPEEVAKIEAEQPKEPEPLLEPQPEPTPSVDPAVYIMEEVIKPNLREASIREEVQPSFIYATEIANFKLEFDRGTERWHEAEWESSDLTDAERIVYKCYMAPNEQPKEDVVDVAVESSVNSLPTAKAQEESAPEVPIEAQKIENTEVTQPETVNEELRQQEKLETPLVITSETISETVSQTANESDKQKSIENNLQIKQNVAEVQVQSDDQPSTSRDANRRAKRSQQSRKGRSREQSQKPTNRTKLPNNIDQKVNESKTAAKETEAVKDKDLSAAASNIQSDVTASDPKTSTPLKILSEGTNSNTLETMENVTSTDINDNVTIEVISNRSEEVPAIQDLGKTKDISEPTANPIEEITSIQNSTTISEQSEGPQEPEIPIEVSETTEALQVPREEASIEIVSPPNEEQTKSPTSQEVNIQDTSHIISLPITDVTPTPEIINIAPSTSSISKEQKQSPKRLSKIPVRTLSSSSLRSESRSSNRTPTANDEIEREETTSQGVPIGETVSSPKSEQLSDNQEVNLVSQETQSKKDTNIVEEPATQPLGLELEEHSPNATAVAVSPTDSDEVFEDAPEFSGSDGTRPHDETASDAELYSLDSDGQRAETKSPEDEVVILLDEESQMESSIAQSESNASLDSHSSESETSKVVLKEFVPSGDPAKQNLSELVEDTQRLIKQMRDEISMDEFESTDEDEYSDEYSDEYDEGEEEEWYDSEGEEEGDFDGEEGNTYNEHASYIEEASTGDEGTEIEDIMEEDEDLADDDEPLQSQIPLDIEPVISPALSVTPTNQETDTIAHTEVVSSTGTRLETELPNPAMESILPSQSVQEDIKVEAIPIQSAPPIADSETRPAEQPVELVLEIPSEVEPTPVEEPTALPITPAPPIVDSESRPVEPPVETVLEEPKKVTPSMKGKTANSGTASKGPSTSSSTKTNKSTVSKIPKPTNEPTNKSNSTPLNKKVPLRSKSFSAPMGISSVKRIQEVYLQKQSSSIATSRVPLKSSPVTKKSINDAISRFNSNQADGPSTSGAAAAAAAALLKPRSQPRIPKKKYHETCFSDDDYETSATEEEQEEPNLAEPQKAEQLKRKMSMPVFRAYPSVQEPVIEDPAILARKYVDQELVTNIAEAQIAATLVSMKFSEDVALWAARECSDLDQAIAMLQQECELCMNSYPMNQMVSMLKCLHKCCKQCAKSYFTVQITDRSINDCSCPFCKLPELSNEAQHEDEHLEYFSNLDIFLKSILDNDVHELFQRKLRDRSLLQDPNFKWCIQCSSGFFARPKQKRLICPDCGSVTCAQCRKPWERQHEGSSCEAYLEWKRENDPELQAQGVQEHLAQNGIDCPKCKFRYSLARGGCMHFTCTQCKFEFCYGCARPFMMGAKCTVSTYCAKLGLHAHHPRNCLFYLRDKIPLQLQFLLKEQNVKFDTEPMQIKDESSSSSKARAQARCPIPLQKETPQGLVDTVCNTEVPDKHAGMCRTHYVEYLAGKVAKAGIDPLPIFDLTDCVQELRRRGIALPERGPWDTDEIYKNMCSEVIKKHIPLKSA