Protein: Guanylate cyclase (TgGC) (EC 4.6.1.2) (Guanylyl cyclase) (TgATPaseP-GC) (S7VVK4)
Organism: Toxoplasma gondii (strain ATCC 50853 / GT1)
Function: Catalyzes the synthesis of the second messenger cGMP from GTP. During the tachyzoite lytic growth cycle in host cells, detects and transduces environmental changes in potassium, phosphatidic acid and pH levels. By producing cGMP in response to these environmental changes, activates PKG and thereby regulates PKG-dependent microneme secretion which is essential for tachyzoite motility, host cell attachment invasion of and egress from host cells. May play a role in the fission of connected tachyzoites at their basal pole during egress. Does not display flippase activity towards phosphatidylserine, phosphatidic acid or phosphatidylcholine.
Subcellular location: Cell membrane {ECO:0000269|PubMed:30449726, ECO:0000269|PubMed:30742070, ECO:0000269|PubMed:30992368, ECO:0000269|PubMed:31235476}; Multi-pass membrane protein {ECO:0000255}. Note=Localizes to the apical cap in extracellular tachyzoites (PubMed:30449726, PubMed:30992368). During tachyzoite intracellular replication in the host cell, localizes to the apical cap, to the cytoplasm in disperse foci and to the residual body (PubMed:30742070, PubMed:30992368, PubMed:31235476). {ECO:0000269|PubMed:30449726, ECO:0000269|PubMed:30742070, ECO:0000269|PubMed:30992368, ECO:0000269|PubMed:31235476}.
Interaction partners:
Sequence:
MKKTRTTAAERSQRARKRPHDEHRGRGREHGGARDPGAGGQAMKGAEKGRNPKHQATQKQMSFLQGKHQQRVGNVVSRLGPAAVGQTTLMDKKRKLYSDTSQQPLWSLRKITINPTDKDDLRRFPSNAIYTHRYSAMTFIFKNLWEQFHRVINWWFLVMAIIQAIPQLHYNPNHAWSTALPFAIVLVFGMLKDAFTDLGRRERDRVLNQRVCCIVDGHTPQLRLLQWQGVRVGNILRLTDGEEVPADIVVLATSNTDGVAYVETSKLDGETNLKFKQGVKETRGESSPLSIAGIRGRVVCEKPCAVMDAFTGSLKLDAHPRATPLDIVNFIQRGSHIRNTEWLYGVVIYTGEDTRIQKNAAPPGFKRPHIEKDINTYLFISFFIVFLTILISVMSKWSVQERDSGDTGVTDAGASSGSGSSSGETSQTYGSSVEFMLGSRDLLQNPWMSILRFLAVYAPVLPLSLPLILDVVYLLQSVLIEGDIHIRGGGRTPATGRGNGSTSSMTVADDGLPDGEAGGLDSAHSSQNASLQQPLSIVSKGFSKTRRFLSEKFSSPSSGQIGGNQDRRETPREDDERETNGENVPSATPLLPGGEAACVATGDEETLRADAEGAQERDREAEGNREQLKSVPTGTHAAWRPGALDSTAEANEDEDPDVLHAEKASGFGARRSDDRDRKSSCSRYPETGGEKTSHAISGRFTGSVMPSTYTKLHGDIANAGFESAWQPYANGHTHTRTLLLATGGGPNSLCATGNFEREDVWAEVHTPALNPNLGQVDFIFTDKTGTITENDMTFSMCSVAGKIYGMASCGGAGYEGAESSEYGSRVWTANETSARSSRDRLVPHLQRGTSAASSRSQSAPASANDEGTPRRIPTLMLRPQTLTNQQTGQQSPVLPASREDEKGKDASPGAADSPASSLSVSRFFGSVSYEGRLEETGSQKEEDSRSDRVSLSPSEGRVSGSRPQLACEGIHGATLRRRTVGIQSQHSSQSLLSSRQQSEDERQYTEGEEGETEDDRSTVSRRGRDRMYSRDYHRESRSSSPRDGELSDRYPLRCIRSIGDGYIIRGGRTGNVVMRHPSGMSFDSRPSSQFTFSSPVVGDDSFDDPRRAMYFGRGSPRSLTPPSERGTASPSVGEEGPPLSPQISPTGSARGRVPRSSSLYMPAVDEGEDRERYFRPRRRMLRPGASLLPPLSPSPSRAGVPFLYDNIDFPTGFRETPMEGQIRRNCDFYDASIFTDLARKDLRSHRINEFIKCMALCNTVVPWVYTATPCSSSCPGLPSTANFSVAAYPTGGGANSLFSCSASAFAGPVTQGFPSVTHPAALASDGAAGADEDEEKNVSQLLLPSGTSASSGAPSGPRGDPQLVSLLGRQGQGHGSLGAPGSGLPTSGCLGGAGGSGARGPMGLHGSRRSLPGSSDCRVPSTARPAPLLGSDAAGRGFGPDYVPSPRPLSPAGPPNASTGSLGEAPKADKPASPEEAASPGKDGEEAPSSTPRCVFAPSTLVPPSVLRSHTGAGGLRPSKSLARGVSFKEKHEEFAFSKDEDTATVDQDDTQSATDEEHDVEGEEEEAGKSAERLTKAKRNRSASASLMSLKSKVTASGRDVADLLFRRGSRGNSGTDPDPASARTVGRSSSVERAQQPPTHGGFSTVTGTGESRSSLVSIIKYQASSPDEECLVSAASHMGYTLVSRTNNYAILNINGQERRWQIIGVNEFTQKRGRMSIVVRPQEWTEGSILYVKGADVAMLDLLSTSFTRGHDFRQSFSCLSRYAESFSGGRGPQVSLSRLQSNSSAKGSGGPPVRRSVSGSDPRLQPSRSVSLPRTVPPGQETPMAGDQLEGPEAAETVNPSGQTYSQGEEEEGLRSRLARADSMLRSLNPALYNYDKQHQRGHGPEGDEGSHELEGHDAHTGDSHGGHHRDQAEPRAGDAVGPESASRLPQKTQNRLPQHLGNAGVCAHGHAWTPSEENYCAAFFDDDEDELIALGDIALVEQHLKRFSLQGLRTMALACRYLTQEETETYKRLYTDACASVYCRAERLEEVAEDMERDLEYLGITGVRDKLQEQVPETLQLMMEAGIRVWMVTGDNVEYALHICHSCRLLTSRTRIFHAALEFSGRKAKREGVMLYELFRKARRLKRSDEHICLVVTGPNLRTFLNHPDLQTYFLNMACCSDVVVAARVTPSQKAEMVRLVKKRLTPQPITMAVGDGGNDVAMLQEAHVGVAIRGKDSAAAVAAAYADYSFTEFRFLQRLLFVHGRLSLMRVSTVILWSFFKSLCIGLPTFLFQPQAFWSAVEVYDPLLLMIVDFFWTTLPGIIHGYSDQDLPTHLLPSVPVLYTPGRRRLYFNGFRFILWTVEGIIYSFLIFYLLQATWMDGNTFHDGQVLGFHSYGILLLFGSLLQSNVRIILETSLWTPTFLFTTIVLCTIMFFPTVLLYSVTGWPRRYMELAGRVVFAWPMLYFLIPLWVSIGILVQLLLQVFTSSLFPNISGSVKQYLAQKQADVNYRRKASKHPFSQPRPRLLPGGHDEYGLFCGMGSCWSLFKRSLWFMGGCCLMDTPNVADDFTASPALCAELRERFNPLRRKSVADRLPPPRRFRINENFLSYTGASEEKGADGVQRDLGGKGSACGPGAAGLAAGTGAPGAGRSKSVFGGGGGVMPMADMAADLETENSLSSNGSEQEKAGDNVTKLVKVSHLINRFTLRFKDMQLEADYQIHNKKSFLKRLVPWYRVIFMLIALYQLLSFLTEYFIDIHWNPGETEMEPWMCVPTLVVEIGFAAVVVCTFYDFIFLDHFSLILNSIVFLMVSSSIVFYTASHVDGTLTSVLFPVFTFVILRISFLQAVVWNILFLIVTVARFMLDKKYLPPLNFVHYIPLFIGIDVFVAFVGYRLEYNQRKSFLLDYSVDASRRKQREILNTMLPSFVVDQMINSELNEEGIPTSLKAEDRGTVSVIFCDVYEFQHVVASIEPTRLVEVLDSLFLCFDRSAEQFGCTKIETVFETYLAAAGLQPGREASPASYQQDACDALDMALAMLEVAAQIRYEVKSNQGVLSGSAVGSSSAVGPAVSASNLEASGNHMEPRLSLHGHNSGVAMGSRMNSSRFHRQKTGSVVRTVLQSRPQRIRVKIGIHSGRVISGVVGAKKPQYALFGDTVNTASRMKTTGQPGYIHISEDSYELVKGDDTLEYESRHTEVKGKGLMNTYLLVRVKGSPYPHFDDQEADEGDVISETGGQNGESRRSTASLPRQLETAGASSGVHTGEAMGCFASVGSRGESQTAADAAAAEAIEEDIQVEIDEEGVVEDTVEKAVEDVRRLRNEGSHAVASDDFPVSQAGAAQPRRCSRFLFQNAEEHGAAEPQRGEGARLILSPGQTEGTRQSEKEGSALGSNIAASARADRRPAGRREDSRGDSRFDYESMTTQQLLRIYRRQQKVAKVLQWIDEELRGQRDTAHRHARTADIVAKLLASSGDEQAAGESSEADHDEVPLDEIKEELRRQAREANEQESAKRSGGDAPPHTPTRKALLKAFRSEIVHGCAAPTEEEKTAKEGRESEVALASPETESRDANGQRVSERDASDAREGSASPQPDHAAPPSGLPQGLKGQETEKQQGWKLFRSASLVRAAGQSFASLFRRRKPAAPSEAASPSSADTPMDSRVSPTSVDDEDLEQNRVTRIGVSSEWLLLKFKDKNLEARYRTHFYNNKSNINTIEQALIIFLVTFCVQTLTRLALPRFYVVCSHHTINLHVCTGLYWAVRATYTLAAFVLWMLFHYRNRKEVATCLELRWMVFLLNLLFISASCVFALSNSWGVCGQQQEDHGASETAVENTLVALSHLAKDGSFPADDAEVNSVAGSPLAQAMHSPFTTAGVSRASRMLRSGGVSVSGGTDCTGVGTSGEEGSDLVTANGRAYTYWLLSDTIELFFYIVILHHNTGLLFQNCILVDVLLMTMSLTFIITTARETASTVSTIATFPCYVFFNLVSAYCKEYIDRLTFYVNEHAKTTESRATQLLNDMLPKQVLEEFQQDKLKLAYLHENVTFLFADICGFTSWAKGVDACEVVTMLQKLFAKFDKDSTKFGLYKLCTIGDAYVAVSEPVTAENAQDTDPREGMWLVYEMAKAMIGNITEVRERLCIPNLNMRIGLHYGSCVGGVIGSGRLRYDLWGMDVLTGNMMESNGVPGKINVSEILKNEMEKGFPGEFVFKFNKTVAVLQSTVDSYLIRPAKDFDEDEELAAAAATMAVAGPSASLQQGGGAIPQVQAVASGLRRDATSIRGNYRRRFTILGSAPRVLGRRQSLRGHQFSALALASHGDSGPSDEPRHLGDEGQAAGSVTSHDGPLREEVEDDEIDGLKQLRKEIERAGGLGLDASPSDIGSTPGSALGS