Protein: LEM protein 2 (Ce-MAN1) (MAN1 homolog) (Q9XTB5)
Organism: Caenorhabditis elegans
Function: Nuclear lamina-associated inner nuclear membrane protein that is involved in cell division, nuclear structure organization, maintenance of nuclear envelope integrity and nuclear envelope reformation after mitosis. In interphase cells, plays a role in anchoring and spatial arrangement of chromosome arms at the nuclear periphery, forming so-called lem-2 subdomains. Both arms of autosomes but only the left arm of the X chromosome are anchored in lem-2 subdomains; sequences bound by lem-2 are mainly repetitive chromosome sequences and inactive genes. Involved in chromosome segregation and cell division, probably via its interaction with the nuclear intermediate filament protein lmn-1, the main component of nuclear lamina. Required to organize the distribution of lmn-1, nuclear pore complexes (NPCs) and chromatin in mitotically active cells. Involved in the nuclear positioning and efficient anchoring of microtubule-organizing centers (MTOCs) to the nuclear envelope during mitosis as well as on maintaining correct nuclear morphology. Contributes to closure of nuclear envelope (NE) holes and prevents excess nuclear membranes after meiosis and mitosis. Together with emr-1, plays a role in baf-1 enrichment at the nuclear envelope in anaphase. Together with emr-1, involved in muscle cell attachment to hypodermal cells, as well as muscle cell location and sarcomere organization. May play a role in radiation-induced DNA damage repair response.
Subcellular location: Nucleus inner membrane {ECO:0000269|PubMed:10982402, ECO:0000269|PubMed:12684533}; Multi-pass membrane protein {ECO:0000269|PubMed:10982402, ECO:0000269|PubMed:12684533}; Nucleoplasmic side {ECO:0000305}. Nucleus envelope {ECO:0000269|PubMed:10982402, ECO:0000269|PubMed:16950114, ECO:0000269|PubMed:21176223, ECO:0000269|PubMed:25653391, ECO:0000269|PubMed:32271860, ECO:0000269|PubMed:35852146}. Chromosome {ECO:0000269|PubMed:16950114, ECO:0000269|PubMed:25653391, ECO:0000269|PubMed:32271860}. Note=Remains in the nuclear envelope until mid-late anaphase and reassociates with the chromatin periphery at telophase (PubMed:10982402). Recruited to the reforming nuclear envelope initially at the apical surfaces of chromatin, from where it spreads to the lateral surfaces facing the spindle microtubules (PubMed:25653391). Requires mel-28 for chromatin reassociation after mitosis and for nuclear envelope localization (PubMed:16950114). Localizes to the nuclear envelope at non-core regions after mitosis (PubMed:35852146). In meiosis at anaphase II, appears on the chromatin surface farthest from the extruding polar body (PubMed:32271860). After anaphase II, forms a plaque on the oocyte-derived pronucleus adjacent to the meiotic spindle (PubMed:32271860). Later disperses into a uniform rim around the oocyte-derived pronucleus (PubMed:32271860). {ECO:0000269|PubMed:10982402, ECO:0000269|PubMed:16950114, ECO:0000269|PubMed:25653391, ECO:0000269|PubMed:32271860, ECO:0000269|PubMed:35852146}.
Interaction partners:
- Lamin-1
- Barrier-to-autointegration factor 1
- Cell division cycle 5-like protein
- Chromo domain-containing protein cec-4
- Ankyrin repeat and LEM domain-containing protein 1 homolog
- CTD nuclear envelope phosphatase 1 homolog
- Putative aminopeptidase W07G4.4
- Nucleoporin Nup54 alpha-helical domain-containing protein
- Smad protein daf-8
- INTegrator complex Subunit homolog
Sequence:
MVDVEKMSDAELRAELNVRGANVGPVTGTTRSLYEKKLKKLLSGGAKTPARPTVAKPAPKPTPKSAPAPKSPKSPPARRSIPRAAATAANSTINSTFNRSEIEEMSDSDDDMRDDDDDDEEILSPKSKQSSFRSANSTASSVGRGRPVSSTPNKRLSPVYKPSPVPKNTPRTTSSSSKTTINTTTTRIPSTPRRITSVPGLITDFTPSFSTFGSDRPGATPPRKSIYTSKVSKVLHDLGNTTGEEDDDDEFEGQETSRIIYKTEEPSRRGIVKNAWNKVLGYGFDASKNPGDSYDLRAGASRIRVQKNPRTGKVTVKQTNIFNEAIYFALYVILILFVVLGIAYALTTTHRPKTADFSGYWGVLKAAGRDSLNFFYNYAILPVVSLGIFVVLGAGIYFGHRKYKEAKEQEEAKLYELIERITELIRESSIDGDPYVSQPHVRDVLFPPAKRRSAELARWEQAVKFIDTNESRVATDVLVLPSGNECAVWKWIGNQSQKRW