Protein: Autophagy-related protein 2 (Meiotically up-regulated gene 36 protein) (O94649)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Lipid transfer protein required for autophagosomes completion and peroxisome degradation. Tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion. Atg2 binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, using basic residues in its N-terminal region (NR) and to the expanding edge of the IM through its C-terminal region. The latter binding is assisted by an atg18-PtdIns3P interaction. Atg2 then extracts phospholipids from the membrane source using its NR and transfers them to atg9 to the IM through its predicted beta-sheet-rich structure for membrane expansion. Atg2 is also involved in the recruitment of lipids to a restricted region close to the vacuole, termed the vacuole-isolation membrane contact site (VICS), which is also essential for autophagosome formation (By similarity). May have a role in sporulation.
Subcellular location: Preautophagosomal structure membrane {ECO:0000269|PubMed:23950735}; Peripheral membrane protein {ECO:0000269|PubMed:23950735}. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P53855}; Peripheral membrane protein {ECO:0000250|UniProtKB:P53855}. Note=Localizes to the IM-ERES contact site. {ECO:0000250|UniProtKB:P53855}.
Interaction partners:
- Serine/threonine-protein kinase atg1
- Autophagy-related protein 9
- Autophagy-related protein 18
- Putative lipase atg15
- Ubiquitin-like protein ATG12
- Autophagy protein 5
- Autophagy-related protein 21
- Probable cysteine protease atg4
- SVP1-like protein 2
- Autophagy-related protein 8
Sequence:
MRLPSWLKNSSSWLWTAALSRTVQRRLLAFALRKLIGSILLENVQPEDIDILFSKGVLMLSNLQLNCSFLNAVVSLPMINFTKGTLRRLILRLNVTDIVNLNVELEVNGLSLEIELVPPDESLSSTTYEDAPSQLDILDNVVEYMNKTASQDFEDEVINEGLESEIDGSSHNLLDSILQKCLASTSVLMQDALVYIGTANMSTRLEAKLDFMSFSSVKSNSTSRLLNINGITVSMVRPISKSNAGSSSPPRSEESFVSMDSSSSIVEGFENDLSPSQVTLNESSIISSNREEESFYSVHDSVTQKKTRTSWLIFQCSGEFRLVFSIESSNLLVIESHVPSCVLNINSQVIAFLLYLYGYFLPAPSTPGFSSNKPPLTMLQLDIHISSVQILTHCKLPESQDFSMHNDVDELLHTIPSNGAVFEMKINQIQIFNDNNDIDELTMTFSDLDIFMDSVTLVSFGKSLQSPCSLIFKKLERIVSLFIHIPGGEISLPLGKALLLQESFVEFTNDISNLQNFLSNSDPFKRSIQETFEAPKPFEVEMNQPSFEIIALFDELQLQILNELSTQSLYKLTLRVSHLQFTRKSTGSLSSVLIFVKKIGCQSELFNCENSDWTKVSSSTAFHLSNSLFETHDTTGTSNFAVSIQQEKHYYPVFQPAPTEFSYPEKHFYFAVDNFNVFISKEVVRLFKTLYETIASSLVTPVTPNKLVTSDYKNVLKIRTRTFSLSLLNDDGSSFALKCIRIKHYMCWAGAQLISLSLRLYNVSAEYLSESLEILPVVSFIRNLRNDEKYLLNADFSYALKRSSGSNDNTIFVKITDLGYEHYPTCPWISDLLKTYFPQDPEVPFLAFPDFPFNIKLDLRESIIGLNPRTLEAKLLLYLKSLDVEIDALVASNPLNIRIMAAETVVYIIDKLNQSVLEGKTSVLKREILNSSLHFPGLSIKDTIEFVVKSLGYVEISQLKNLTLSLVVNAEEGVFSTLITVDNLDAQVQSCADSTELLIKVLSDLGSTEDEEISDCYLALPIEDYAKSLTEVDYNFFENRGIDYKSNPTEQSTVLVSSDNDISSQEIKIVDDYYISSNEHSTHASDLASVSSEEFVIDDGSSSIIDISDELQDESSSRDSLKKGIELIEDYYLSQSTSKLESSVEGKNYLLKVKFKDINVNWDLHDGYDWEATRATISSAIEKLCDSSSQNDKISPEAKTLLFQSIVIKSFSKVGRLNINHVSEPIDSDEFADYLSKSISYHLRLGKSKSKKIGIEIKDLQGSFTVYADSNEPNAVLNDLDIGLKDLIIYDHLSTSTWNKFFGRDSRSPSSKNRNQHMNAQIVTVRPLPELNNRELRLEFSVLPCKMYIDQDTLDFLIRFLTFQTPSSSETLNTEPDLPFFQSICINATHVTIDFKFKSADKVGLRSGKLPDLGSLIVMQGSEVFLRQLQIYGLSGAEEFLNALLNVWLQDIRNNQLSKVLNGVVPIRTMFTVGRGIKDIFVSPVRGLQGNHSVGSFRHGIIKFTEKYVNDFLSLNAQGATGTHSLLRQAESYLERGSNASASASRARSYYAEQPETIEQGLRQGYSGLKQGLLGAKSTLMGLPRETRSHKSLGGVAQTVGRKVPLIVLQPMIGATEAVSKTLLGLSNSLQPQRRQDMREKYKRPG