Protein: Protein mini spindles (Q9VEZ3)
Organism: Drosophila melanogaster (Fruit fly)
Function: Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Promotes cytoplasmic microtubule nucleation and elongation. May act as a microtubule antipause factor that rapidly catalyzes the transition from pause to either growth or shrinkage. Involved in mitotic spindle elongation. Involved in the establishment of cell polarity and mitotic spindle orientation in neuroblasts. Required for maintaining the bipolarity of acentrosomal meiotic spindles; the function is dependent on tacc and involves ncd. Involved in oocyte microtubule cytoskeleton organization and bicoid mRNA localization. Seems to be involved in elongation of kinetochore-derived microtubule fibers.
Subcellular location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:10477755, ECO:0000269|PubMed:26953351, ECO:0000269|PubMed:33725482}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:10477755, ECO:0000269|PubMed:11433295, ECO:0000269|PubMed:33725482}. Cytoplasm, cytoskeleton, microtubule organizing center {ECO:0000269|PubMed:32066907}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:32066907}. Note=Localizes to the plus ends of growing microtubules in the cell interior in a Eb1-dependent manner (PubMed:21965297). Localizes to the lattice of growing and shrinking microtubule in a discontinuous pattern in the peripheral regions of interphase cells (PubMed:21965297). Localized at acentrosomal poles of female meiotic spindle (PubMed:11433295). In embryos, associates with centrosomes in prophase and metaphase, then associates with spindle microtubules (PubMed:33725482). In the fat body, localizes to a perinuclear non-centrosomal microtubule-organizing centers (ncMTOCs) (PubMed:32066907). {ECO:0000269|PubMed:11433295, ECO:0000269|PubMed:21965297, ECO:0000269|PubMed:32066907, ECO:0000269|PubMed:33725482}.
Interaction partners:
- Transforming acidic coiled-coil protein, isoform K
- Kinetochore protein NDC80
- Protein claret segregational
- Wee1-like protein kinase
- Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
- Tubulin beta chain
- Centrosomin
- Tubulin alpha-4 chain
- Tubulin beta-2 chain
- Tubulin beta-3 chain
Sequence:
MAEDTEYKKLPVEERCVHKLWKARVDGYEEAAKIFRELDDEKSPEWSKFAGLIKKMVVDSNALAQEKGLEAALIFVENSGLAGRTVGDVMTGIVQKCIAAPKTKTKELSVQVALMYVEIEKQEAVVEELVKGMEAKNPKIVSACVAATTLALREFGHKVIGVKPLIKKLAPLMSDRDKTVRDEGKQLAVEIYRWIGAAMKAQISTLPQVTLKELEDEFDKLKGERVEPSRYLKSQQEKQAKIADAAATEDAYNEDDGEAGVEEIDPMDLLDPVDILSKMPKDFYDKLEEKKWTLRKESLEVLEKLLTDHPKLENGEYGALVSALKKVITKDSNVVLVAMAGKCLALLAKGLAKRFSNYASACVPSLLEKFKEKKPNVVTALREAIDAIYASTSLEAQQESIVESLSNKNPSVKSETALFIARALTRTQPTALNKKLLKLLTTSLVKTLNEPDPTVRDSSAEALGTLIKLMGDKAVTPLLADVDPLKMAKIKECQEKAEIKIKVAGPKKETRPASAPTAKAAAPAKTVAGSVDPKPVTRPATTGARKVLKKPATVSGGGATSAPTAALKAGGKPLATEREITPEELQEKSEEILPAEILNGLVDSNWKNRLAAVEQLLGEISGFDAKQAGISQILIRTISGRKPGLKEMNFQVLKFKLDIIRSVAENYPLTTTTVDLVINEIIEKLADAKNGAAAADVLSAFAEATKLEYVVGKVLSFAFEQKSPKVQSEAFNWVNRSIIEFGFQLQPKTLIEDVRKGVQSTNPTVRASAIQMVGTMSMYMGKALMMFFDSEKPALKSQIQVEFDKNVGEKPPKPVRGVQRSSGGTAGNSPDNEDDDGGAAGEEEPINMADLLPRVDIAPQITEALLKEMSDKDWKTRNEGLTKLQAIISEARLIKPSIGDLAPALAHRLVDSNAKIAQTTLAICEQLATAMGAGCRNHVRNLFPGFLHALGDNKSFVRAAALNCINSFGEKGGYKEFFESEMIADALKGGSPALKTELWAWLADKLPGLPPKSVSKEDIHSMVPHLYAHICDRNADVRKNANEAVLGIMIHLGFDAMNRALDKQKPASKKDILAALEKARPNLPVKPLPKGKHQAPIPEEPKLKTVRGGGAGGAPGIQKSATARVAGGQDKQVPARKKDEDIDTSPLLCANSAKNQRLLDEQKMKVLKWTFVTPREEFTELLRDQMMTANVNKALIANMFHDDFRYHLKVIEQLSEDLAGNSKALVCNLDLILKWLTLRFYDTNPSVLIKGLEYLVQVFQVLIDEEYILAENEGSSFVPHLLLKIGDPKDAVRNGVRRVLRQVILVFPFVKVFGYVMEGLKSKNARQRTECLDELTFLIESYGMNICPQSAVREIARQISDRDNSVRNAALNCIVQVFFLSGEKTYKMIGHLNEKDLSMLDERIKRAKKTKKPTPPPSVDVPAPQRHDSIEIEDAEVGNGCDELPPPDEDGTFDQAPSSQLLLLQQQLQQLQQQAQQQKPSGPFGLDSQVISEIEKDWVRVDQMEQKPLLNVDISSLDEPIKVRPTRAGIHYPQEKFDRLISRQHYMQQTLTTSPSSTAGMTSGVSPYRSPMRLQHQQPQQQLENNIPNLADVLPKHDPQLVKVIKGVSSTDTLKARAAINELAAIIEAPEKQAVLRDYEEIFIQNVLAQFKNLSQIPSAQSVVVYQPLLSILYTFFHANILGKTLSVACIKNLMSALLNLMADPKLAVGDDSQYNKVINGICLKVLDKVDFTNLNCALIRLLRETCPEAKLPKFTDLLMKCIWRNVKMLPERSNELNYDAVILEVHEFMLALPSTWWQNRPSDTPMRTIKTILHNMAKVKGNAILQHLNQIPTHSELHTYLIRILKNFQKDGSASGIGASPQRAKEIASKRISHQTHDTVSQIFKLISDRDTKQQGLQKLYDFKQQNPDIDLSTFLQGSSAPFHKYIEEGLAEIERNQNAGSTQDNRTDVNYQNNGPDPDFWMDRLQYHMTGGAAKLASARSADDGSHMLDNKVVDENLCLNGMNAQKASLIKREKRDMSPNRLQHLQAKLAQIKKENHAQ