Protein: Germinal-center associated nuclear protein (GANP) (EC 2.3.1.48) (GC-associated DNA primase) (Q9WUU9)
Organism: Mus musculus (Mouse)
Function: As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores (By similarity). Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:10733502, ECO:0000269|PubMed:20507984}. Nucleus {ECO:0000269|PubMed:10733502, ECO:0000269|PubMed:20507984}. Nucleus envelope {ECO:0000250|UniProtKB:O60318}. Nucleus, nuclear pore complex {ECO:0000250|UniProtKB:O60318}. Nucleus, nucleoplasm {ECO:0000250|UniProtKB:O60318}. Chromosome {ECO:0000250|UniProtKB:O60318}. Note=In stimulated B-cells, selectively targeted to immunoglobulin gene variable regions (PubMed:20507984). Predominantly located at the nuclear envelope, facing the nucleus interior (By similarity). Localization at the nuclear pore complex requires NUP153, TPR and ALYREF/ALY (By similarity). Also found associated with chromatin (PubMed:23652018). In B-cells, targeted to the immunoglobulin variable region genes (PubMed:20507984, PubMed:23652018). {ECO:0000250|UniProtKB:O60318, ECO:0000269|PubMed:20507984, ECO:0000269|PubMed:23652018}.
Interaction partners:
- Centrin-3
- Transcription and mRNA export factor ENY2
- PCI domain-containing protein 2
- Centrin-2
- 26S proteasome complex subunit SEM1
- Nuclear pore complex protein Nup153
- DNA replication licensing factor MCM3
- Nucleoprotein TPR
- Nuclear pore complex protein Nup98-Nup96
- Nuclear RNA export factor 1
Sequence:
MHPVNPFGGQQPSAFAVSSSTTGTYQTKSPFRFGQPSLFGQNSTPSKSLAFSQVPSFATPSGGSHSSSLPAFGLTQTSSVGLFSSLESTPSFAATSSSSVPGNTAFSFKSTSSVGVFPSGATFGPETGEVAGSGFRKTEFKFKPLENAVFKPIPGPESEPEKTQSQISSGFFTFSHPVGSGSGGLTPFSFPQVTNSSVTSSSFIFSKPVTSNTPAFASPLSNQNVEEEKRVSTSAFGSSNSSFSTFPTASPGSLGEPFPANKPSLRQGCEEAISQVEPLPTLMKGLKRKEDQDRSPRRHCHEAAEDPDPLSRGDHPPDKRPVRLNRPRGGTLFGRTIQEVFKSNKEAGRLGSKESKESGFAEPGESDHAAVPGGSQSTMVPSRLPAVTKEEEESRDEKEDSLRGKSVRQSKRREEWIYSLGGVSSLELTAIQCKNIPDYLNDRAILEKHFSKIAKVQRVFTRRSKKLAVIHFFDHASAALARKKGKGLHKDVVIFWHKKKISPSKKLFPLKEKLGESEASQGIEDSPFQHSPLSKPIVRPAAGSLLSKSSPVKKPSLLKMHQFEADPFDSGSEGSEGLGSCVSSLSTLIGTVADTSEEKYRLLDQRDRIMRQARVKRTDLDKARAFVGTCPDMCPEKERYLRETRSQLSVFEVVPGTDQVDHAAAVKEYSRSSADQEEPLPHELRPSAVLSRTMDYLVTQIMDQKEGSLRDWYDFVWNRTRGIRKDITQQHLCDPLTVSLIEKCTRFHIHCAHFMCEEPMSSFDAKINNENMTKCLQSLKEMYQDLRNKGVFCASEAEFQGYNVLLNLNKGDILREVQQFHPDVRNSPEVNFAVQAFAALNSNNFVRFFKLVQSASYLNACLLHCYFNQIRKDALRALNVAYTVSTQRSTVFPLDGVVRMLLFRDSEEATNFLNYHGLTVADGCVELNRSAFLEPEGLCKARKSVFIGRKLTVSVGEVVNGGPLPPVPRHTPVCSFNSQNKYVGESLATELPISTQRAGGDPAGGGRGEDCEAEVDVPTLAVLPQPPPASSATPALHVQPLAPAAAPSLLQASTQPEVLLPKPAPVYSDSDLVQVVDELIQEALQVDCEEVSSAGAAYVAAALGVSNAAVEDLITAATTGILRHVAAEEVSMERQRLEEEKQRAEEERLKQERELMLTQLSEGLAAELTELTVTECVWETCSQELQSAVEIDQKVRVARCCEAVCAHLVDLFLAEEIFQTAKETLQELQCFCKYLQRWREAVAARKKFRRQMRAFPAAPCCVDVNDRLQALVPSAECPITEENLAKGLLDLGHAGKVGVSCTRLRRLRNKTAHQIKVQHFHQQLLRNAAWAPLDLPSIVSEHLPMKQKRRFWKLVLVLPDVEEQTPESPGRILENWLKVKFTGDDSMVGDIGDNAGDIQTLSVFNTLSSKGDQTVSVNVCIKVAHGTLSDSALDAVETQKDLLGTSGLMLLLPPKVKSEEVAEEELSWLSALLQLKQLLQAKPFQPALPLVVLVPSSRGDSAGRAVEDGLMLQDLVSAKLISDYIVVEIPDSVNDLQGTVKVSGAVQWLISRCPQALDLCCQTLVQYVEDGISREFSRRFFHDRRERRLASLPSQEPSTIIELFNSVLQFLASVVSSEQLCDISWPVMEFAEVGGSQLLPHLHWNSPEHLAWLKQAVLGFQLPQMDLPPPGAPWLPVCSMVIQYTSQIPSSSQTQPVLQSQVENLLCRTYQKWKNKSLSPGQELGPSVAEIPWDDIITLCINHKLRDWTPPRLPVTLEALSEDGQICVYFFKNLLRKYHVPLSWEQARMQTQRELQLSHGRSGMRSIHPPTSTFPTPLLHVHQKGKKKEESGREGSLSTEDLLRGASAEELLAQSLSSSLLEEKEENKRFEDQLQQWLSQDSQAFTESTRLPLYLPQTLVSFPDSIKTQTMVKTSTSPQNSGTGKQLRFSEASGSSLTEKLKLLERLIQSSRAEEAASELHLSALLEMVDM