Protein: Cytochrome P450 1A1 (CYP1A1) (EC 1.14.14.1) (CYPIA1) (Cytochrome P450 form 6) (Cytochrome P450-C) (Cytochrome P450-P1) (Cytochrome P450MT2) (Hydroperoxy icosatetraenoate dehydratase) (EC 4.2.1.152) [Cleaved into: Cytochrome P450MT2A; Cytochrome P450MT2B] (P00185)
Organism: Rattus norvegicus (Rat)
Function: A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15alpha and C16alpha positions (By similarity). Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation (By similarity). Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system. Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer (By similarity). May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (By similarity). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (By similarity).
Subcellular location: [Cytochrome P450 1A1]: Cytoplasm {ECO:0000269|PubMed:9348277}.; SUBCELLULAR LOCATION: [Cytochrome P450MT2A]: Endoplasmic reticulum membrane {ECO:0000269|PubMed:9348277}; Peripheral membrane protein {ECO:0000305|PubMed:19401463, ECO:0000305|PubMed:9348277}. Mitochondrion inner membrane {ECO:0000269|PubMed:9348277}; Peripheral membrane protein {ECO:0000305|PubMed:19401463, ECO:0000305|PubMed:9348277}. Microsome membrane {ECO:0000269|PubMed:9348277}; Peripheral membrane protein {ECO:0000305|PubMed:19401463, ECO:0000305|PubMed:9348277}.; SUBCELLULAR LOCATION: [Cytochrome P450MT2B]: Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion inner membrane {ECO:0000305|PubMed:19401463}; Peripheral membrane protein {ECO:0000305|PubMed:19401463, ECO:0000305|PubMed:9348277}. Microsome membrane {ECO:0000269|PubMed:9348277}; Peripheral membrane protein {ECO:0000305|PubMed:19401463, ECO:0000305|PubMed:9348277}.
Interaction partners:
- UDP-glucuronosyltransferase 1A1
- UDP-glucuronosyltransferase
- UDP-glucuronosyltransferase
- Cytochrome P450 3A1
- Catechol O-methyltransferase
- UDP-glucuronosyltransferase 2B37
- UDP-glucuronosyltransferase 2B15
- Epoxide hydrolase 1
- UDP-glucuronosyltransferase 2B1
- Transmembrane O-methyltransferase homolog
Sequence:
MPSVYGFPAFTSATELLLAVTTFCLGFWVVRVTRTWVPKGLKSPPGPWGLPFIGHVLTLGKNPHLSLTKLSQQYGDVLQIRIGSTPVVVLSGLNTIKQALVKQGDDFKGRPDLYSFTLIANGQSMTFNPDSGPLWAARRRLAQNALKSFSIASDPTLASSCYLEEHVSKEAEYLISKFQKLMAEVGHFDPFKYLVVSVANVICAICFGRRYDHDDQELLSIVNLSNEFGEVTGSGYPADFIPILRYLPNSSLDAFKDLNKKFYSFMKKLIKEHYRTFEKGHIRDITDSLIEHCQDRRLDENANVQLSDDKVITIVFDLFGAGFDTITTAISWSLMYLVTNPRIQRKIQEELDTVIGRDRQPRLSDRPQLPYLEAFILETFRHSSFVPFTIPHSTIRDTSLNGFYIPKGHCVFVNQWQVNHDQELWGDPNEFRPERFLTSSGTLDKHLSEKVILFGLGKRKCIGETIGRLEVFLFLAILLQQMEFNVSPGEKVDMTPAYGLTLKHARCEHFQVQMRSSGPQHLQA