Protein: tRNA nuclease CdiA (tRNase CdiA) (EC 3.1.-.-) (Anticodon nuclease CdiA) (CdiA-EC536) (Toxin CdiA) (Q0T963)
Organism: Escherichia coli O6:K15:H31 (strain 536 / UPEC)
Function: Toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. CDI modules allow bacteria to communicate with and inhibit the growth of closely related neighboring bacteria in a contact-dependent fashion (target cell counts decrease 100- to 1000-fold). CdiA toxicity is neutralized by its cognate immunity protein CdiI, but not by CdiI from other bacteria. Uses heterotrimeric OmpC and OmpF as target cell outer membrane receptors; receptor function depends on polymorphisms in extracellular loops L4 and L5 of OmpC; interacts with itself and closely related bacteria but also with OmpC from E.cloacae ATCC 13047. Its ability to preferentially bind to 'self' receptors suggests it may also play a role in self-recognition and kin selection. A bamA mutation that decreases its expression about 5-fold is partially resistant to this strain of CdiA, probably due to decreased outer membrane receptor protein assembly. Isolated CdiA-CT is imported in an F-pilus-mediated fashion; CdiA-CT inhibits F-mediated conjugation, probably via its N-terminus (residues 3016-3097), although it is not clear if this is physiologically significant. Gains access to the cytoplasm of target cells by using integral inner membrane protein FtsH. The C-terminal domain (CT) cleaves within tRNA anticodon loops; this activity is inhibited by cognate CdiI. tRNase activity of CdiA-CT is stimulated by CysK, although the extreme C-terminus (residues 3098-3242) has tRNase activity in the absence of CysK. In vivo CDI toxicity requires CysK. CysK stabilizes CdiA-CT, allowing it to bind tRNA substrate; neither CdiA-CT nor CysK bind tRNA alone in vitro. Purified CdiA-CT (residues 3016-3242) inhibits E.coli cell growth when added to cultures alone or in complex with cognate CdiI, growth is inhibited when cognate CdiI is present within the cell but not when a CdiA-CT/CdiI complex is added extracellularly, suggesting CdiA-CT alone but not the CdiA-CT/CdiI complex is imported into the target cell.
Subcellular location: Secreted {ECO:0000269|PubMed:23469034}. Target cell membrane {ECO:0000269|PubMed:23469034}. Target cell, target cell cytoplasm {ECO:0000269|PubMed:23469034, ECO:0000269|PubMed:24889811, ECO:0000269|PubMed:25174572}. Note=Secreted to the cell surface by CdiB, its two partner secretion pathway (TPS) partner (Probable). Expressed on the cell surface of both the encoding cell, and in mixing experiments on the target cell surface, in a proteinase K-sensitive manner. Both the N- and C-termini can be detected on target cells, whereas only the CT domain is detected in the target cell cytoplasm (PubMed:23469034). Toxin translocation into the target cell depends on the proton motive force of the target cell, but not on tolA or tonB (PubMed:25174572). {ECO:0000269|PubMed:23469034, ECO:0000269|PubMed:25174572, ECO:0000305}.
Interaction partners:
Sequence:
MHQPPVRFTYRLLSYLISTIIAGQPLLPAVGAVITPQNGAGMDKAANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLKAGGEAKGIINEVTGGNRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTTGRPVMNADGSLQALEVTEGSITINGAGLDGTRSDAVSIIARATEVNAALHAKDLTVTAGANRITADGRVSALKGEGDVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARDGDIILSSAGKLVLKNSLAGGNTTVTGTDVSLSGDNKAGGNLSVTGTTGLTLNQPRLVTDKNLVLSSSGQIVQNGGELTAGQNAMLSAQHLNQTSGTVNAAENVTLTTTNDTTLKGRSIAGKTLTVSSGSLNNGGTLVAGRDATVKTGTFSNTGTVQGNGLKVTATDLTSTGSIKSGSTLDISARNATLSGDAGAKDSARVTVSGTLENRGRLVSDDVLTLSATQINNSGTLSGAKELVASADTLTTTEKSVTNSDGNLMLDSASSTLAGETSAGGTVSVKGNSLKTTTTAQTQGNSVSVDVQNAQLDGTQAARDILTLNASEKLTHSGKSSAPSLSLSAPELTSSGVLVGSALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSGLITGDNGLMLNAVSLSNPGKIIADTLSVRATTLDGDGLLQGAGALALAGDTLSQGSHGRWLTADDLSLRGKTLNTAGTTQGQNITVQADRWANSGSVLATGNLTASATGQLTSTGDIMSQGDTTLKAATTDNRGSLLSAGTLSLDGNSLDNSGTVQGDHVTIRQNSVTNSGTLTGIAALTLAARMVSPQPALMNNGGSLLTSGDLTITAGSLVNSGAIQAADSLTARLTGELVSTAGSKVTSNGEMALSALNLSNSGQWIAKNLTLKANSLTSAGDITGVDTLTLTVNQTLNNQANGKLLSAGVLTLKADSVTNDGQLQGNATTITAGQLTNGGHLQGETLTLAASGGVNNRFGGVLMSRNALNVSTATLSNQGTIQGGGGVSLNVTDRLQNDSKILSGSNLTLTAQVLANTGSGLVQAATLLLDVVNTVNGGRVLATGSADVKGTTLNNTGTLQGADLLVNYHTFSNSGTLLGTSGLGVKGSSLLQHGTGRLYSAGNLLLDAQDFSGQGQVVATGDVTLKLIAALTNHGTLAAGKTLSVTSQNAITNGGVMQGDAMVLGAGEAFTNNGMLTAGKGNSVFSAQRLFLNAPGSLQAGGDVSLNSRSDITISGFTGTAGSLTMNVAGTLLNSALIYAGNNLKLFTDRLHNQHGDILAGNSLWVQKDASGGANTEIINTSGNIETHQGDIVVRTGHLLNQREGFSATTTTRTNPSSIQGMGNALVDIPLSLLPDGSYGYFTREVENQHGTPCNGHGACNITMDTLYYYAPFADSATQRFLSSQNITTVTGADNPAGRIASGRNLSAEAERLENRASFILANGDIALSGRELSNQSWQTGTENEYLVYRYDPKTFYGSYATGSLDKLPLLSPEFENNTIRFSLDGREKDYTPGKTYYSVIQAGGDVKTRFTSSINNGTTTAHAGSVSPVVSAPVLNTLSQQTGGDSLTQTALQQYEPVVVGSPQWHDELAGALKNIAGGSPLTGQTGISDDWPLPSGNNGYLVPSTDPDSPYLITVNPKLDGLGQVDSHLFAGLYELLGAKPGQAPRETAPSYTDEKQFLGSSYFLDRLGLKPEKDYRFLGDAVFDTRYVSNAVLSRTGSRYLNGLGSDTEQMRYLMDNAARQQKGLGLEFGVALTAEQIAQLDGSILWWESATINGQTVMVPKLYLSPEDITLHNGSVISGNNVQLAGGNITNSGSSINAQNGLSLDSTGYIDNLNAGLISAGGSLDLSAIGDISNISSVISGKTVQLESVSGNISNITRRQQWNAGSDSRYGGVHLSGTDTGPVATIKGTDSLSLDAGKNIDITGATVSSGGTLGMSAGNDINIAANLISGSKSQSGFWHTDDNSASSTTSQGSSISAGGNLAMAAGHNLDVTASSVSAGHSALLSAGNDLSLNAVRESKNSRNGRSESHESHAAVSTVTAGDNLLLVAGRDVASQAAGVAAENNVVIRGGRDVNLVAESAGAGDSYTSKKKKEINETVRQQGTEIASGGDTTVNAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLETKKKSGGFLSKKTTHTISEDSASREAGSLLSGNRVTVNAGDNLTVEGSDVVADQDVSLAAGNHVDVLAATSTDTSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSASTIGSTAGNVSITAGKQAHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSGLTVALSGTVGSAINNAVTSAQETKESSDSRLKALQATKTALSGVQAGQAATMASATGDPNATGVSLSLTTQKSKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGSQLKVGGNTSLDAANDILLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFAGVNAAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGNRIIADVGHDLLISSQQDTSKYDSKQTSVAAGGSFTFGSMTGSGYIAASRDKMKSRFDSVAEQTGMFAGDGGFDITVGRHTQLDGAVIASTATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDKFQGNMPGGMISAGGHSGHAEGTTQAAVAEGTITIRDRDNQKQNLANLSRDPAHTNDSISPIFDKEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAAQDKYGPVPADATEEQRQAYLAKLRDTPEYKKEQEKYGTGSDMQRGIQAATAALQGLVGGNMAGALAGASAPELANIIGHHAGIDDNTAAKAIAHAILGGVTAALQGNSAAAGAIGAGTGEVIASAIAKSLYPGVDPSKLTEDQKQTVSTLATLSAGMAGGIASGDVAGAAAGAGAGKNVVENNALSLVARGCAVAAPCRTKVAEQLLEIGAKAGMAGLAGAAVKDMADRMTSDELEHLITLQMMGNDEITTKYLSSLHDKYGSGAASNPNIGKDLTDAEKVELGGSGSGTGTPPPSENDPKQQNEKTVDKLNQKQESAIKKIDNTIKNALKDHDIIGTLKDMDGKPVPKENGGYWDHMQEMQNTLRGLRNHADTLKNVNNPEAQAAYGRATDAINKIESALKGYGI