Protein: Inactive histone-lysine N-methyltransferase 2E (Inactive lysine N-methyltransferase 2E) (Myeloid/lymphoid or mixed-lineage leukemia protein 5 homolog) (Q3UG20)
Organism: Mus musculus (Mouse)
Function: Associates with chromatin regions downstream of transcriptional start sites of active genes and thus regulates gene transcription (By similarity). Chromatin interaction is mediated via the binding to tri-methylated histone H3 at 'Lys-4' (H3K4me3) (By similarity). Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages including G1/S transition, S phase progression and mitotic entry. Recruited to E2F1 responsive promoters by HCFC1 where it stimulates tri-methylation of histone H3 at 'Lys-4' and transcriptional activation and thereby facilitates G1 to S phase transition (By similarity). During myoblast differentiation, required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells.
Subcellular location: Chromosome {ECO:0000250|UniProtKB:Q8IZD2}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:Q8IZD2}. Nucleus speckle {ECO:0000250|UniProtKB:Q8IZD2}. Note=Absent from the nucleolus. Localizes to chromosome during interphase and to centrosomes during mitosis. Dissociation from mitotic chromosome is likely due to histone H3 phosphorylation on 'Thr-3' and 'Thr-6'. {ECO:0000250|UniProtKB:Q8IZD2}.
Interaction partners:
- F-box-like/WD repeat-containing protein TBL1X
- F-box-like/WD repeat-containing protein TBL1XR1
- Histone-lysine N-methyltransferase SETD1A
- Nuclear receptor corepressor 1
- Histone H3.2
- Histone H3.1
- Histone-lysine N-methyltransferase SMYD3
- Nuclear receptor corepressor 2
- Histone-lysine N-methyltransferase SETD1B
- Histone-lysine N-methyltransferase 2C
Sequence:
MSIAIPLGVDTTETSYLEMAAGSEPESVEASPVVVEKSNSFPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSGLISKNEVGIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVPKVTDKRRKKSGEKEQNFSKCKKAFREGSRKSSRVKGSAPEIDPSSDSSNFVWETKIKAWMDRYEEANNNQYSEGVQREAQRLAQRLGSGNDSKDMNKSELSTNNSLFRPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEEGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENPECPVLKRSSESTENINSGYETRRKKGKKEKDTSKEKDIQNQNMTLDCEGTNNKIRSPETKQRKLSPLRLSVSNNQEPDFIDDMEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKPECKESQVIADAEVVQEQVKEETAIKPAAAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIPPSSPDIEVLSQQNEIENTVLAIEPETETAVAEIIPEAEVPALNKCPTKYPKTKKHLVNEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKKRRKETTENISGSCKKRWLKQALEEENSTILHRYHSPCQERSRSPTVNGENKSPLLLSDSCSLPDLTTPLKKRRLYQLLDTAYSESSTPTPSPYATPTHTDITPTDPAFATPPRIKSDDETYRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRCTYNQEGYDRPSNMLTLGPFRNSNLTELGLQEIKTIGYTSPRSRTEVNRPCPGEKESVSDLQLGLDAVEPAALQKSMETPAHDRTEPSNQLDSTHSGRGTMYSSWVKSPDRTGVNFSVNSNLRDLTPSHQLETGGGFRVSESKCLIQQDDTRGMFLGAAVFCTSEDGLASGFGRTVNDNLIDGSCTPQNPPQKKKVSLLEYRKRQREARKSGSKPENFALISVSPHPSGSLSSSGDGCVHSSENGEQAENQASLPLPPPAAAAAATAAAAYSASSEEGSSNCPVKDANSSEKKDPEVQWTASTSVEQVRERSYQRALLLSDHRKDKDSGGESPCVSCSPSHVQSPPSSHSNHIPQVHAQSLAPSLSELMADPDAEGTEATSTSECPSPDTSQSPSKTSKPGSPGPINPAQSHGKILTKPDSHWEATATVSEADNSVHQNPEPQHRQLSSNTPALSQNHAPQAHALSANDQLPQKLPSAPTKLHCPPSPHTENPPKSSTPHTPVQHGYLSPKPPSQHLGSPFRPHHSQSPQVGTPQRETQRNFYAAAQNLQANPQQATSGALFTQTPSGQSSATYSQFNQQSLNSTAPPPPPPPPPSSYYQNQQPSANFQNYNQLKGSLSQQTVFTSGPNQALPGSTSQQSVPGHHVTPGHFLPSQNPTIHHQPAAAAVVPPPPPPPPAPGPHLIQQPSSHQQHSVAHGVGPVHAVTPGSHIHSQTAGHHLPPPPPPPGPAPHHHPPPHPTTGLQSLQAQHQHVVNSAPPPPPPPPPPPPASVLVSGHHSASGQALHHPPHQGPPLFPASAHPAVPPYPSQATHHTTLGPGPQHQPSGTGPHCPLPVAGPHLQPQGPNSIPTPTASGFCPHPHPGSVALPHGVQGPQQASPVPAQIPIHRAQVPPTFQNNYHGSGWH