Protein: Serine/threonine-protein kinase ATM (EC 2.7.11.1) (Ataxia telangiectasia mutated homolog) (AtATM) (Q9M3G7)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR) or DNA replication stalling. Plays a central role in the perception and response to both stress-induced damage in somatic cells and developmentally programmed DNA damage during meiosis. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates histone variant H2AX to form H2AXS139ph at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Involved in transcriptional regulation of RAD51, PARP1, GR1, and LIG4 in response to DNA double strand breaks. Plays a dual role by activating the DNA damage response at dysfunctional telomeres and yet preventing this activation at functional telomeres. Not required for telomere length homeostasis. Regulates DNA damage response (DDR) synergistically with RUG3. Together with RUG3, involved in the splicing of the ND2/NAD2 mRNA.
Subcellular location: Nucleus {ECO:0000305}.
Interaction partners:
- DNA repair protein RAD50
- Nibrin homolog
- Serine/threonine-protein kinase ATR
- Double-strand break repair protein MRE11
- Protein BREAST CANCER SUSCEPTIBILITY 1 homolog
- Cell cycle checkpoint protein RAD17
- Probable inactive protein kinase At3g63330
- Chlorophyll a-b binding protein 3, chloroplastic
- ATP-dependent DNA helicase 2 subunit KU70
- E3 ubiquitin-protein ligase PRT1
Sequence:
MKLQNPDKKTLREGFSQESSVVALDSGVLAMSGLKCDGKFPVKDVLMEEGGDKVRKIQVSGGNISLVVDFSGARTSSNNFFESNASCVNENLVKGNGYREDETQEFLVGNLVWVMTKYKKWWPGEVVDFKADAKESFMVRSIGQSHLVSWFASSKLKPFKESFEQVLNQRNDNGFFDALQKAMSLLSNSLKLDMTCSCIADGNGIVSAQNITTRKNKPLILREFSVDRLEPKEFVTQLKNIAKCVLNAGVLESTVMQSQLSAFYTLFGHKQIPMAQLHENEGRKSFTAKMSDSKFIGSPSICAGNSRKRFRKEWFRKFVSEVDNVSARDDLVNVPPSDLISKLKLLAVGYNCSEETENIGLFEWFFSKFRISVYHDENAYKMQLANMAGFKDLMLATNANRGTVQKTLKSKKIGKSKMEPLNGVSVADTEQKTFELQISKKSNIESLNGVSVADTEQKTFELQILEKSNIESLNGVSTPNIDHEASKSNNSGKTKINHIIGHSNFPSSVAKVQLAKDFQDKLLVQAPDRKAMTADTLSRPAAILVPDLNSGGNALGTAEFDHMQRPETLIQHNVCPQEEKTPRSTILNFQVTAHQGVSGTQFVSSQPTSYKHFTSADLFTYSGKKKRGRKRKNAEELPIVAHASATTGIPDLNGTNTEPTLVLPQVEPTQRRRRRKKEESPNGLTRGITILFLKFSSQVSMPSRDDLTSTFSAFGPLDSSETHVSEEFSGAQVAFVSSADAIEAVKSLEKANPFGETLVNFRLQQKLITVQRNIAPRMPVISHVSPVPKPNNIPTSMDAMRQNLLMMTAMLEKSGDSLSRETKAKLKSEITGLLEKDGVKLLNTWLEGERSITFCRFLSQNTAKLKLDEIPNAETWPFLVKLLLQCVSMEVSGSKRRMPKPTFAKTLRVVVQRTEETKFPGVQFPLLSMAKTLFTHVHDILSNTPSFQSEYGTILRHLLEIKEYRFQMRKRTYSSLVLLYMERAETGFCEKNSGQHSQKEEAFRYILTLQSLLENSPGDFPDDLREEIVNGLIHIFSSVRDEGKLSRKLIECVNTFLLKDGPNLGSLSLEIHNAVEQFVFRCWLTTHDKNLKEILVSYGRLQLNLTRDSSESSSLVEQLLDVVTRELDLGSSSSSASWGDTTKDEKLGALSSYQNSLVELAAHVFYRACVNTSRPSLSEKRARRQHIAMRMVDALTEGKWLWCAAFGCLVRNYCARINMDLLIYWFEAICTNFQRLLEDASMRRSYDGLLWTLRSLQGLSSGLSLPDITMDISKSSASSSELDRGWQSIWSSLIHGLATFSSMSVIVDAVLVLLGSIISSNHITVKILPQEVWDHQLFRHIPSEPALYFIACYFSRMGCQGNLQDDLHLRRNLLRAVCAPLSWKVRLTLDERMVQLLPAAAFSLCAGFKVSLPLPKEHLPTPSQWDVCEQIDDVDRERNFGLFECSVEALTRICSNSSKISGCQVPDVVQLPLVLRDPLLHDMDIYFLSIIPEVKEKGPLSDIFMGCALLCHFMHGSYITRKGKGSSSFFLKACQYLLEGLDHAVESVSKSLNDLQRRGSLGFGSDFNEKGSIIVSLRSFTQSPVFSNRRDQNLLGASYDFVIHSLENLLRSFAKVYEEYTEHAWNTHSDTVPSKSLAPDSPEVGRIVDMDLDLAEDTKERDIIAAGGKAVPGLPVSMGNWKLGMVSLISCFSPVLQFPTWDVLYNLLEKESDPKVLENILYHLCKLSCLTSIPKVDDLVIFLDGMLSTQVKMKRNCLNIVTALHVLLHTLSSSRRDSSGVEKNCGLSLKEAESFQVFVQLGAMVNKVSEFGLLGWFGRVKLINCICDLVLLNPQTGQTMIERLLLMLSDSDYRVRFVLARQIGILFQTWDGHEALFQDICSSFGIKLVTSSKEKLVTAKDVLAVGPQPRQKMETVIITLMHLAYHSENIELQAVFMMCAVSAKDPCQRELIIAALDNLSAQLHYPSRFKYLEELLGPILFHWIASGVSLAGLIETSQLFIPNAEPKYFIHFCSHWLLPALLLHEDHTNLDWVAKMAGQPVVVLVKENFVPIFSICMGLHCSKTSECDKGAMVLQNSILYVGETSENERDKLIKQNMVSIVSFILSCASSSPEPPVPTFSRDTISLAVQTVVDGFLENTDYPKNAAITDRINIFRPDRVFMFITEMHYRMSAACHHRHTRHHLAALEELTILLGHRALVPSSLNYIFNLVGQFIGYPSLQDQCCSIASCLLDLFKSNPAKEIVSVLGDQLQFLVSKLVTCCIDAEADTKISGAKSSQLVNLLHKLVVSSDSSLNEDIRDLEPLPDLKYFQVIRESHIRICEAYSPRNHLLKVEHSTFLIYIFLEILSLSNFLFLSCSTIQQCSRRSNYLPPRFLSRSLQALHNKLIASEVSQEDTNGETAETFWQSDDEIVNAVWTLVRVSASDEADSMRLLVSDFLSRIGIRDPHTVVFHLPGNLVSMHGLQGFGHNTGSKVRSLTENGISDETLITLLNFLKKYLLDDSVKIIDVTSQTLRGILSTERGQQALSSFDSCERALIEVHGRGVNLDIVEKILLDSQKQFKAEKFSLETPEVWSTDNKNFDRWICQLVYCMIALCEDVPIRLCQNIALLKAEISELLFPSVVVSLAGRIGMDINLHDLITSQVKEHIFTDSNKLTKSKQVMLNTLNELRMCYVLERSIFSGQTKREKNSRSCSTAAKIRDVESGSNGMAASITTNWEKVYWLSIDYLVVAGSAVVCGAYLTASMYVEYWCEEKFGNLSLGDPDFSYHDKLPDHVEILVSAITRINEPDSLYGVIHSNKLSAQIITFEHEGNWTRALEYYDLQARSQKMVVPSSLSENLEVEQFQPTTSARHSVFGEGEVQRQPFKGLIRSLQQTGCMHVLDLYCRGLTSREGCFQYDPEFIELQYEAAWRAGKWDFSLLYPQTHCQPLQHAKNNNYHESLHCCLRALQEGDYDGFYGKLKDTKKELVLSISRASEESTEFIYSTVVKLQILHHLGLVWDLRWTTSSHQSVHGYLVKQMACVDPVIPTMDQLSWLNKDWNSIITQTQLHMTLLEPFIAFRRVLLQILGCEKCTMQHLLQSASLLRKGTRFSHAAASLHEFKFLCARSNGQQPVPDWLGKLEEAKLLHAQGRHEVSISLANYILHNYQLKEEASDIYRVIGKWLAETRSSNSRTILEKYLRPAVSLAEEQSSKICKRLVDRQSQTWFHLAHYADALFKSYEERLSSSEWQAALRLRKHKTKELEVFIKRFKSSKKAEQSDYSLKIQDLQKQLTMDKEEAEKLQVDRDNFLKLALEGYKRCLEIGDKYDVRVVFRQVSMWFSLASQKNVIDNMLSTIKEVQSYKFIPLVYQIASRLGSSKDESGSNSFQSALVSLIRKMAIDHPYHTILQLLALANGDRIKDNQRSRNSFVVDMDKKLAAEHLLQDVSHYHGPMIRQMKQLVDIYIKLAELETRREDTNRKVALPREIRSVKQLELVPVVTATIPVDRSCQYNEGSFPFFRGLSDSVTVMNGINAPKVVECFGSDGQKYKQLAKSGNDDLRQDAVMEQFFGLVNTFLHNNRDTWKRRLAVRTYKVIPFTPSAGVLEWVDGTIPLGDYLIGSSRSEGAHGRYGIGNWKYPKCREHMSSAKDKRKAFVDVCTNFRPVMHYFFLEKFLQPADWFVKRLAYTRSVAASSMVGYIVGLGDRHAMNILIDQATAEVVHIDLGVAFEQGLMLKTPERVPFRLTRDIIDGMGITGVEGVFRRCCEETLSVMRTNKEALLTIVEVFIHDPLYKWALSPLKALQRQKETEDYDGMNLEGLQEEFEGNKDATRALMRVKQKLDGYEGGEMRSIHGQAQQLIQDAIDTDRLSHMFPGWGAWM