Protein: RNA editing associated helicase 2 (EC 3.6.4.13) (ATP-dependent RNA helicase REH2) (Q581T1)
Organism: Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Function: ATP-dependent RNA helicase that unwinds RNA in a 3' to 5' direction and that plays an important role in mitochondrial mRNA editing, a process involving the addition and deletion of uridine (U) nucleotides in the pre-mRNA. As part of the RET2-containing gRNA-binding (RET2-GRBC) complex, acts as a scaffold for the assembly of mRNA-gRNA hybrids and the recruitment of the RNA editing core (RECC) complex. Regulates several steps of mRNA editing by the MRBC3010/GRBC6 containing gRNA-binding (MRBC3010-GRBC) complex including loading of unedited mRNA, editing in the first sequence block and subsequent editing progression across multiple sequence blocks. Also, regulates the RNA substrate content of the MRBC3010-GRBC complex as well as the association of this complex with mitoribosomes.
Subcellular location: Mitochondrion {ECO:0000269|PubMed:19850921, ECO:0000269|PubMed:25928631, ECO:0000269|PubMed:26769962, ECO:0000269|PubMed:31034523}.
Interaction partners:
- REH2-associated factor 1
- REH2-associated factor 2
- EF-hand domain-containing protein
- RNA editing complex protein MP63
- Guide RNA associated protein, GAP2
- RNA-editing ligase 2, mitochondrial
- Mitochondrial oligo U binding protein TBRGG1
- RNA editing 3' exouridylylase MP99
- RNA-editing ligase 1, mitochondrial
- RNA-editing-associated protein 1
Sequence:
MRAIRLTVACRYLGPFRSVTLSPVVLPVRLFQTQEITPGEVSLVEATDESDRAFGETRKEDFSFDGSSEFPECFKDTKHVDTFSRARVVSFIKRFSQGAVTSSSEVFEGREIVNDNGTPLYHSRVRLPFRSHTGELWAHGVACNSKDAELLAAMHAEHIIDEFGYHIYTLPSMQRKHAEAARKAGRWAPLPDELERTQSPVRVPLPLRRIVDRDETEGGKWLLIDMRPNHYISPSHTLLSPCLFDTTAVHRIKSFLDEHKLSFAQLCTSVEEPGEGGGQSWYVATVSLPPELSTFSEIKAQGKALNREAAVTLACMHAELVLDAHSICLYPSDSTKQKQHALAAWSYGRPAPLPGEDQKNPSHVVCPLPLKQLAVRREDRISCISYEEDIIRRHRALTDQTCEFIETPTLDSSAVEQLKQFLQRENVPRTDPFLVEEVNGYYKATVVLPLPDLYGIRGGVGIAMNATDARVLAAMHAIDVLNILGFHLMDGSTARAEWIAARRARGESVPADTRDPNVLSPSGRRRVATGNSSTQTPSSSTNARSVAAAPDVGTSDPTAPQNTKRRVAKRARVADAQPTEEKDAANSDSDEATSYLKMRETVSKELWNLEPDSPDGYIMVSPTDPETRTQFEQALYSPRQVDLGSKSRIKNYLASVGRRIEEVFFVQRIEAEDNGGQAICRCAVNLPVPRRFGDRIALGEAVDPKDAENLAAMHAELILDTLGIPIYTDSALQRLHVRLCAKCGRNAPVEYSESVAAATASPPPLRREVVGSIHWENKSKRRRAAISVQKGGGPANSQETTSALREDEEPLIAPKERREYTFVPEKDLDLVSRARVHYYLRRNGIAKLEPEYRMELRGLGNVLHIAELTLPLPDVYGKRVAHGSALTKRDAEILCWMHAEQILDAVGLCLFDNLPMLQRRHVECVKRLGRWAPLVSENATKPPHTPTPLPLTLGTTQEKPQYPTPPTNVRQDWEQYAQECQRYIEINVMREHNIFYEMGKTPRTGDETYDAALAEVESMPIDPDAKTVLQRYCNVANVNYPTFWKSRTVGPISCRVCLTTIEVPGHEYLRASGVAWNKEASQRQAAMHALALLRRVEPDFAEFEKQIKAEVVDKVNLVDPAAVLDEEAPVLRRTARVSKKSLGNWDPVSKDFSHEGKVRIIELFTVCFGLQPPLVRHLNRRSGSFVQHFTVVEVTDEDGKTWVGTGRDAGPRFNEPAAFDDLFSKLSRGVQGFQALMDLIRAHPHLDPEHIANVSLTDSQKERILKAVDGLPMVEEEDVAHPEQWADADSDRGIGIMALIAMDASQRAQESQELEAKLQAKLTNEEYQTRYASQRQRLRIYEKRDEILRAISSNQIVIICGTTGCGKTTQVPQYILDDMTEKGMGGDCSIVITQPRRLSAVSIARRVAAERLESIGETCGYSIRLDAKPGRNINFCTSGVLLRLLHSAPLLNGINYLIIDEIHERDINSDFLLILLRQLLHRRKDLHVILMSATLQADQFGKYFGNAPIINVEGYVHAVEEMYLEDLVPIATERNVMTPLLKEAAAALERNGAADGFCPTVVPPTAKYGFLEATADIDYMTIQIAIDHAVRSLDLTDSSILVFLPGWDEINRAKEILERNAKFHIICLHSSVGAEEQMRCFLPAPEGKIKLILSTNIAESGVTIDDVAAVIDVGRGKEKSYVMRKGTTSVGRNEMGSMSQLVTVYASRANCVQRRGRVGRTRPGMCIRLYSKKHFQSLHDFQTPEMLRTHLDSLCLQILALDLGDPADFLQQALEPPSSDHIEAAMKRLHELGATTSTRQLTPLGLRLSRLPVAPKVGKMVIMGAILRCLDSALTIAGVSDTDVFISTREHREAVRLHKEDLSYGTQSDVIASVNAFNFWVTSHYAKTPAEVVYDLQERMLSVPQLLTVSKYKQQFFEIVAGSGFIHMKQNYKDAKNKDRADIFVDQSEYSADSLNVGLVKCVVASGLFPNVVMNRGKRLMRNKLANRLDPSSASVVHRTSQENIGQPYFVYDELVKSSESERLLVRDLTNVSLWTILLMGTSSMPVTYRDDLNLAVVDEWIMFRATFGTLELIRKFKRALNVCLGRKFMNPNDEENNAKLEELRCIIKELVCTPFKPNDLAEKPWEEKGVIIEPCTEPKGGSSEAEKTHVNSSHTPTTSAEAGGDS