Protein: Cytosolic phospholipase A2 epsilon (cPLA2-epsilon) (EC 3.1.1.4) (Calcium-dependent N-acyltransferase) (Phospholipase A2 group IVE) (Q50L42)
Organism: Mus musculus (Mouse)
Function: Calcium-dependent N-acyltransferase involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain. Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide. Has weak phospholipase A2 and lysophospholipase activities. Regulates intracellular membrane trafficking that requires modulation of membrane curvature as it occurs by enrichment in lysophospholipids. Promotes tubule formation involved in clathrin-independent endocytotic trafficking and cargo recycling.
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:15866882}. Early endosome membrane {ECO:0000269|PubMed:24413173, ECO:0000269|PubMed:29447909}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305|PubMed:24413173}. Lysosome membrane {ECO:0000269|PubMed:15866882, ECO:0000269|PubMed:24413173, ECO:0000269|PubMed:29447909}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305|PubMed:24413173}. Cell membrane {ECO:0000269|PubMed:24413173, ECO:0000269|PubMed:29447909}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305|PubMed:24413173}. Note=Targeted to clathrin-independent endocytotic vesicles through binding to phosphoinositides, especially phosphatidylinositol 4,5-bisphosphates. {ECO:0000269|PubMed:24413173}.
Interaction partners:
- Polyunsaturated fatty acid 5-lipoxygenase
- Polyunsaturated fatty acid lipoxygenase ALOX15
- Arachidonate 12-lipoxygenase, 12R-type
- Mitogen-activated protein kinase 3
- Phospholipase A and acyltransferase 3
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
- Polyunsaturated fatty acid lipoxygenase ALOX8
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
- Protein kinase C alpha type
- Mitogen-activated protein kinase 1
Sequence:
MQSIPHSDEADVAGMTHASEGHHGLGTSMLVPKNPQGEEDSKLGRNCSGFEDAQDPQTAVPSSPLLSMASCSSQEGSSPCHLLTVRIIGMKNVRQADILSQTDCFVTLWLPTASQKKLKTRTISNCLHPEWDESFTFQIQTQVKNVLELSVCDEDTLTQNDHLLTVLYDLSKLCLRNKTHVKFPLNPEGMEELEVEFLLEENFSSSETLITNGVLVSRQVSCLEVHAESRRPRKRKKNKDLLVMVTDSFENTQRVPPCQEPCYPNSACFHYPKYSQPQLYAEAPKSHCNFRLCCCGTHRNDPVCQPLNCLSDGQVTTLPVGENYELHMKSSPCSDTLDVRLGFSLCQEEVEFVQKRKMVVAKTLSQMLQLEEGLHEDEVPIIAIMATGGGTRSMVSLYGHLLGLQKLNFLDASTYITGLSGATWTMATLYSDPEWSSKNLETVVFEARRHVVKDKMPALFPDQLYKWREDLQKHSQEGYKTTFTDFWGKLIEYSLGDKKNECKLSDQRAALCRGQNPLPIYLTINVKDDVSNQDFREWFEFSPYEVGMQKYGAFIPSELFGSEFFMGRLMKRIPEPEMCYMLGLWSSIFSLNLLDAWNLSHTSEEFFYRWTRERLHDIEDDPILPEIPRCDDNPLETTVVIPTTWLSNTFREILTRRPFVSEFHNFLYGMQLHTDYLQNRQFSMWKDTVLDTFPNQLTQFAKHLNLLDTAFFVNSSYAPLLRPERKVDLIIHLNYCAGSQTKPLKQTCEYCTEQKIPFPSFSILEDDNSLKECYVMENPQEPDAPIVAYFPLISDTFQKYKAPGVERSPDELELGQLNIYGPKSPYATKELTYTEAAFDKLVKLSEYNILNNRDKLIQALRLAMEKKRMRSQCPS