Protein: Sperm flagellar protein 2 (Protein KPL2) (Q8C9J3)
Organism: Mus musculus (Mouse)
Function: Required for correct axoneme development in spermatozoa. Important for normal development of the manchette and sperm head morphology. Essential for male fertility. Plays a role in localization of the intraflagellar transport protein IFT20 to the manchette, suggesting function as an adapter for dynein-mediated protein transport during spermatogenesis. Also plays a role in bone growth where it seems to be required for normal osteoblast differentiation.
Subcellular location: Cell projection, cilium, flagellum {ECO:0000250|UniProtKB:Q9C093}. Cytoplasm {ECO:0000269|PubMed:19889948}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:19889948, ECO:0000269|PubMed:28619825}. Golgi apparatus {ECO:0000269|PubMed:19889948}. Note=Shows dynamic localization in developing spermatozoa (PubMed:19889948). Localizes to the manchette in step 10-12 elongating spermatids, where it is mainly found on the basal side below the perinuclear ring (PubMed:19889948, PubMed:28619825). Detected in the basal body and neck area of step 13-14 spermatids (PubMed:19889948). Localizes to the midpiece of the sperm tail in step 15-16 spermatids (PubMed:19889948). During the epididymal transport of spermatozoa, expression in the sperm tail reduces and becomes concentrated at the distal part of the midpiece (PubMed:19889948). Detected in the Golgi apparatus of late spermatocytes and round spermatids (PubMed:19889948). Detected in the cytoplasm of Sertoli cells (PubMed:19889948). {ECO:0000269|PubMed:19889948, ECO:0000269|PubMed:28619825}.
Interaction partners:
- Sperm-associated antigen 6
- Cilia- and flagella-associated protein 54
- Sperm-associated antigen 6
- Cilia- and flagella-associated protein 43
- Tetratricopeptide repeat protein 21A
- Leucine-rich repeat and guanylate kinase domain-containing protein
- Armadillo repeat-containing protein 2
- Sperm-associated antigen 17
- Cilia- and flagella-associated protein 69
- Radial spoke head protein 4 homolog A
Sequence:
MSEILCQWLNQELRVSRTVSPKSFAKAFSNGYLIGEVLFKFELQSDFAEFSESRGSTAKLNNFSRLQPTLHLLGLQFDQNVAQSIITEKPGAATKLLYQLYIALQKKKKTGLTGLEIQTMQPQTNLRLQTLKSEAFREQRLNRRRQNEIMAKIQAAIIQIPKPESNRTLKAIEAQKLMKKKKEAEDVANEIKKFEALIKKDLQIKESVSKTSLETTDQTTAELLNTYSDDDYIKKIQKRLEEDAFAREQREKRRRRLLMDQLMAHEAQEEAYREEQLIHRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQFEERRLKDFQDALDREAALAKQAKIDFAEQTLREKEIHEKISVERAQQRYKKHYGICAEILDQMLDLCTKVADYRLLTNNLIPHKMMHDWKELFFSGIPIYEQTSLTHGQTEPTEDHRAEVKKRNLLDSKDYEDYKNMVGEWALPEDMVNSSPPSNNSILGHVLLRLIEKADPSASNAEATELPSLAVKGCILGKTLSGKTTVLKSLQNDFPVHVLSIDTLVQEAIQAFHERQKSGKTPPTQEDDKRDPVVNQEKVSKTQDKNVLAVSPVPGDRTSQKEGVKINEFEQFRSSDSFLSLSMRAQLGAKSELMLRRGKSIPDILLVSILVNAIKEIPVDQSWVLDGFPITLNQAKLLEEALTGYKRKFLQLKKKKEQMPTLALDPSTSTEVSLLPSALDFVILLDISDNSSLARTNDIIAKEISHEISHENVGRPGTGTSQDNKSEDRNLRDHIQHRIVGFLDNWPLLEEWFTQPKNILTKVNAEIDEALLCQKVKEIFATETVNKKIKVEKTLEEKETEKKAGAPPAEPPAMSPPLSSEAEKDKELHQAKTPGKGKTQSVSPKGKAQGGKVSVKKSPVGSAEVSPTPTAPPPPKAGTEEWVYVNEPIPEELPSFLVPYWELIEKSYINHIKTVLRHLRERQHNVLSYLYETRTSFQEFLRRPDHKQDFVSQWQADFNSVPEDLWEDEETKAELHQRVNDLRDRLWDICEARKEEAEQERLDIINESWLQDSIGITMNHFFSLMQAEVNRFQDTKRLLQDYYRAMESKIPLEDSKKFTRVPLVQLDGKEISESQLRIPLVPRISNSPENSAVKPKVGTFLKGRSDPPLEVLEANFEIDEKILLDTWQQASLAISNMVAAEVHQRLTEEEKEPPQLDSKEKSPQSGANKKAKKEKEAPKKKKTDKKGKGKSSPVAEVSPVTVTPEEMAEMEKRNELRLRIKEEHLAALQTEEQAAQFRLELIKLKALSVLEDLVTKVIDVYRLMEKWLGKRYLNEMASIQKLTELARYHIETATKIQNEIYLSQEDFYINGDIKVFPDPSPPTRPPPVEREENGTLTIEQLDNLRDQFLDMAPKGIIGNKAFSDILLDLITLNLGTNNFPSSWMHLSQLDLQEITSLLTVNTEFVDWRKFLMVTAMPWPMALEDELLDTLQRFKALDEAQTGTITYEQYKQAGLWFSGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADCEKEPPQLDYTQMLLYFACHPDTLEGVYRALSVAVGTHIFRQVETPMLMAEKTSISTVSPIEEFPETEESSAKEDRELKEEKDDQKEEEIPENANTEKISMETLLKVFGGGNEVLDANRFASYLKSENIYAENFIKTFQDLGARNLEPIAVNILLKHPYIQDLIANYVDYKFPDIKMILQRSEHAQGSDGERSPSRLTDEKK