Protein: Nesprin-2 (KASH domain-containing protein 2) (KASH2) (Nuclear envelope spectrin repeat protein 2) (Nucleus and actin connecting element protein) (Protein NUANCE) (Synaptic nuclear envelope protein 2) (Syne-2) (Q6ZWQ0)
Organism: Mus musculus (Mouse)
Function: Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. May be involved in nucleus-centrosome attachment. During interkinetic nuclear migration (INM) at G2 phase and nuclear migration in neural progenitors its LINC complex association with SUN1/2 and probable association with cytoplasmic dynein-dynactin motor complexes functions to pull the nucleus toward the centrosome; SYNE1 and SYNE2 seem to act redundantly in cerebellum, midbrain, brain stem, and other brain regions except cerebral cortex and hippocampus. During INM at G1 phase mediates respective LINC complex association with kinesin to push the nucleus away from the centrosome. Involved in nuclear migration in retinal photoreceptor progenitors. Required for centrosome migration to the apical cell surface during early ciliogenesis.
Subcellular location: Nucleus outer membrane; Single-pass type IV membrane protein; Cytoplasmic side. Sarcoplasmic reticulum membrane {ECO:0000250}; Single-pass type IV membrane protein {ECO:0000250}. Cell membrane; Single-pass membrane protein. Cytoplasm, cytoskeleton. Mitochondrion {ECO:0000250}. Nucleus, nucleoplasm {ECO:0000250}. Note=Different isoform patterns are found in the different compartments of the cell. The isoforms having the C-terminal transmembrane span can be found in several organellar membranes like the nuclear envelope, the sarcoplasmic reticulum of myoblasts, or the lamellipodia and focal adhesions at the cell membrane. The largest part of the outer nuclear membrane-associated protein is cytoplasmic, while its C-terminal part is associated with the nuclear envelope, most probably the outer nuclear membrane. Remains associated with the nuclear envelope during its breakdown in mitotic cells. Shorter soluble isoforms can be found in the cytoplasm and within the nucleus (By similarity). {ECO:0000250}.
Interaction partners:
- SUN domain-containing protein 2
- SUN domain-containing protein 1
- Prelamin-A/C
- Emerin
- FH1/FH2 domain-containing protein 1
- Lamin-B1
- Nesprin-1
- Meckelin
- Nesprin-4
- Protein KASH5
Sequence:
MAASPVLPTEDGEGFLGIDDLHFSLQAEQEDTQKKTFTCWINSQLAKHTPPSVVSDLFADIKKGHVLLDLLEVLSGQQLPRDKGSNTFQCRINIEHALTFLKNRSIKLINIHVADIVEGNPSIILGLIWTIILHFHIEKLAQTLSCDYNQPSPEVVSVAASSPTSSPPTKKCSKAQAQARWQWSAKKALLQWAQEQCARSESVNVTDFKSSWRNGMAFLAVIHALRPDLIDMDSMRHRSNKDNLKEAFRIAEHELKIPKLLEPEDVDVVNPDEKSIMTYVAQFLKYSKDAPGPGDSTQAKVRDALVWLTLQEKRFQKMLKDSASETYCNKYHSLLSFMESLNEEKESFIDVLSLKGRMGELNEDESRLRQGWTSLMHQVAAWRAQLDDALPSPLKETEAWLKDIEGVVQEGVPTSQSYSEARTLIQGKLSSFKSLMGSFDYHSDVLMAFQSNAEKSLPAVPPVKLEEMTRRINNVLGKNFIPLLEFHDSKCSVLALLDEAKAKLDVWNGTYESKESVEVLLEDWHKFTGEKKFLIQLDASFQKCEEMYKNSARECESIREEYMMLEKNVHSCRQYIHNTKATLQRALMSWATFEEDLALLKASFDLTKKEQIKEVPVETLLQWNTKHTSLNEVGSFLIGVSSREVAASISKELRRLNKRWRKFITKTPLLKLPLVKIQDQPPGNSSGTSLSKESAMAAEPGGSRGEDVKAAEKQEVEDEESAGQLKVNEEVEGLIKQVTIWESQTKSILDLLQHGDHADGSSADTLQHLIAKGSVYEELLARTEDTLQMDVQSPSNLEPFQNVLRAGLQAKIQEAKQGVQITMVELSAVLKNLSDEPLELDLGLKVEEAQKELEVSILRAEQLLGQRERPGGFLLKYKEALEILNTNSLAKYLRAVEELKRTVPGGAKLQLEEQSRVASAKWEPLRHEISLYLQQLKIAIEEEKLRDNIARLEKQINKEKKLIRRGRTRGLRKEHEACLSPESIKCQLEHHVGVLRVLCEELTSPEDQQELKRALRDYEQKIARLLKCASEIHTTLQSSQGGALEERSALITTENGRRDADGEVPLEIPDNQLSTEKAMEPIKNFSQTSELKPQQEESIMEKEGKDCSASLSDLQERYDTQRGLLEQHLQDSKSRVTSDFASEQERSSACLQSKLAELQVLLADTDAHWEKFEITSLNLRRLMSDAEKPVLNQERDLLKGNEQVLHGLLNTRMESLEMALQIVLPLEKECSLLCASDLPLCTVAVQDLHPVEIDGVYQNLRDIRDSIAKQIRVCTSLEEPSNSVPRELHTLDQCAIQDIVLKCRLQLETMNQKVEMREDALDALEGFLASLRAAKLSAELPADRPAPKAPEVLSEDILLMKEKAGPLDERLRTLGINIKDAEGGENTTCERLVGALSVNLVAMDGQSKEEGPPEDKKLLEACSSKNLELFKNIQDLQNQISKIGLKDPTAPAVKHRKKSLLRLDKDLDGLEEEKVRIQKIAGSLPRFKDGSEKNVIQQCEDTAALWESTKASVTESLEQCGSALELLRQYQNIKNNLTALIQKEEGIISQQASYMGKDNLKKKIAEIETVKEEFSDHLEVVDKINQICKNLQYHLNKMKTFEDPPFEKEANAIVDRWLDINEKTEEYGENLGRALALWDKLFIIKNNIDEWTEQILGKAESHELTEEDRGRLKEELKVLEEQSAEFSRRVADIQSLLQSNEKPLELQVMESSVLSKMKDVKTHVAGGSNSYAPSGSTAELREDLDQAKTQMGMTESLLNALSPSDSLEIFTKLEEIQQQIFQQKHSMTVLENQIGCLTPELSELKRQYASVSNLFNTKKNALQDHFATFLNEQCKNFNDWFSNVKTNLQECFEPPETKLSLEQRLQKLSDFLTLGGGNSKIQQVETVLQHVKMLLPKAHVKELDSWLRSQELELENMESICQARAGELNNSFQQLLRLEDDCRSLSKWLTNQEENWGKMEVSGERMDLFSQALTRKREQFETVAQLSDSLKEHGLTEGEETIKESTHLIDRYQALWRQLHEIEEEDKLPAAEDQSFNDLADDVIHWIKEIKESLMALNSSEGKMPLEERIQKIKEIIALKPEGDAKIQMVMRQAEHCEAPLAQETFTDLSNQWDSTLHLANTYLSHQEKLVLEGEKYLQSKEDLRLMLTELKKQQEAGFALQPGLPEKQAQLKIYKKFLQKAQDLTSLLEELKSQGNYLLECTKNPSFSEEPWLEVKHLHESLLQQLQDSVQKLEGHVQEHSSYQVCLTDLSSTLDDISKEYFSLCDGSKDQIMAKERMQKLQELESRLRFQGGALKKASALAKSIKQNTSSVGQKIIKDDIRSLKYKQKDLENRIESAKQETENGLNSILKSKSSTEKHVKFSLPVEEMPATSEVPKPTRESAAVGESGGARETNTNSAVEMILSKQLSLNVQESMQNAQDEREVNELQNQPLELDIMLRNEQLKGMEELSTHLEARRAAIELLEQSQHLNQTEEQALVLPAARPSVCHLGSLQQELHTLKKTKERQYGLLSGFQDQLVMAEASLNTSLAEVESLKIGSLDSATYLGKIKKFLGSVENQQGSLSKLRTEWAHLSSLLAAADQKLVESQMKHLEHGWELVEQLAHRKCFQQATEHSELTCLLEKLQDLKVSLHQQQQRLTLSLNSPGQQAAIVDMVTPAAELQAIKCEFSGLKWQAELHMKRLWGEKDKKTLEDAINNLNKQMEALEPLNREVENRIKKCELQNRIKETLSWVKNTMAELVVPIALLPDNILSQIRKCKLIHDGILGNQQAVELLVEEVRGITPSLAPCEGDGLNALLEDLQSQHQALLLKSTERSQQLELKLEEKSKLFAIIGKVQLTLEESETLMSPTGDRASTEAELERRLAILKASQQQLQDTESALSAHLQELTNAYKDANVFERLFLDDQLKNLKARTNRTQRFLQNNGSELKQKMESYREFHDKAAVLQKEAECILHGGLLPLRQELEQDAKEQLGNLRDKLAAIRGSLSQVLTSEEVFDTIGLSWDGSLLARLQTQVLEREREVEGKIKQLDTFLIARDRHQASISKIRAVDLQIKKGAESLLKVPSMSPESTLLNAQTLIQKIEKSKRLRDEIIRKLSKNEAFDDSFKESEMQRLKLCAEENSRLQEALQNMLLELQPREMGEKEFREKLENALHVLKQIQSRLQQPLCVNLGVQHIQHEKETWEAFGEQVEAEMCGLRAVRITEEQREENDSGTGGMEAKLRDIEGLHMELSKSISLRADVLNDAYDSANRYDELVAGALRIITSLEATLLSYRVDLHNPQKTLELAHLKQEELQSSVADLRSLTETLGAISSPEAKEQLRCTLEVLAAKNSALKAGLEAQEAEEERCLENYKCFRKMKEEICSRLRKMEMDLGQSIFPLPRSYKEALARLEQSKALTSNLLSTKEDLVKLRQLLRHLRCRSTENDATCALGVASALWEKWLSLLEAAREWQQWGGELKREWKFISEEIEREAIILETLQEDLPEISKTNAAPTEELWQLLDSLCQHQESVEKQQLLLALLLQRVRSIQNIPEGTETGETIPALQEIGSMQERCDRLLHTTRKNKDLVQAEIQAQQSFLKEIKDVKRVFEQISTSFPNLAPEGHPERAEQFEELRSILQKGKLSFENIMEKLRIKYSEMYSIVPAEIGSQVEECRSALEDAEEKMSSEVSKSSPSSIMRRKIERINNGLHCVEKMLQQKSRNIEEAHEIQKKIWDELDLWHSKLNELDSEVQDFVEQDPGQAQEWMDNLMAPFQQHQQVSQRAESRTSQLNKATIKMEEYNDLLKSTEVWIEKTSCLLANPACYDSSRTLSHRASTLQMALEDSEQKHSLLHSIFTDLEDLSIIFETDDLIQTIHELSDQVAALQQQIMEALPHVQQVADDVVAIESEVKAMEKKVAKIKAILLSKEIFDFPPEEHLKHGEVILENIHPMKKTIAEIMTYQVELRLPQTGTKPLPVFQRTSQLLQDVKLLENVTQEQNELLKVVIKQTAECDEEIDSLKQMLTNYSAEISPEHVSQNQVADLPSLQGEMERLEKQILNLNQKKEDLLVDLKTAVLNLHEHLKQEQQEVGDKPSAGASECTVAERDASERKLSRTNSMSFLPVVKEEAEESSVKSEDGRRRTEPPSASWSFLGKHSKDLEGDGASSSSSATIVQDADGRISTCDSSMVHIIAPDSGSTEEGPAPSPRLSQTDEGATPPIEAALLDFPREQGAFESTVERSRPRPADILRVCKTQVAKLELWLQQANVAFEPETVDADMQQVVEEELAGCQAMLTEIEYKVASLLETCKDQGLGDCGTTQQEAEALSWKLKTVKCNLEKVQMVLQEKFSEDQHPSTLKKPSEPHDVDQPAGLSELDSVLTERPQFSRQKDAPQPQILELKPSEQKDLIKFTELNAKKTWLQGHQENEDANRQSASSSKVPSPGNAASDSTLPLQAQSGDKWQYLHHELTSRPNPSVPQLVEPQVALTTSTLPSVSVYNFRCPTADELQAYTTQLEELRQEANTIQTQGSMTEETYISLDKRLFELFLSLSRCLGSVEGLLQRPGLLREDACAQQVFFQKLALELKKLYLALGDKKDDFLKAVTWPGKEATLLPECIDALTVSLESVQSRAAWRDASLKAGLEHSRSYQNEVKRLYSQLIKKKTALQQSLNEISGQSISKQLQKADVHTAELQNSEKQVAKLRDEGERLRFPHGLLQDVYKLEDVLDSMWGILRARYLELSSPFLSKSLQTLLQGMAELVSIGKGKLAADPLQHAKSKAALQAQLQDHKAFFQKLVADMLLIQTYSATMFPPSLQKGEGFGAEQVAEVRALEEEACLRGAQLQSMLQKWEEFDDNYASLEKDLEALISSLPSVSLVEETEERLLERISFYQQIKRNIDGKHARLCQTLNEGRQLAASVSCPEPEGQIARLEEQWLSLNKRIDQELHRLQTLLKHLLSYSRDSDELTRWLETSQQTLNYWKEQSLNVSQDLNTIRSNIDRFFKFSKEVDERSSLKSAVMSTGNQ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