Protein: Toxin Afp18 (Antifeeding prophage 18) (Antifeeding protein 18) [Includes: Protein N-acetylglucosaminyltransferase (Protein O-GlcNAc transferase) (EC 2.4.1.-) (Tyrosine glycosyltransferase)] (C4ULG3)
Organism: Yersinia ruckeri serotype O1 (strain ATCC 29473 / DSM 18506 / JCM 15110 / CCUG 14190 / NCIMB 2194 / NCTC 12986 / 2396-61)
Function: Toxin component of the prophage tail-derived protein translocation system Afp, which is the causative agent of enteric redmouth disease in salmonid fish species. Mono-O-GlcNAcylates the small GTPase RhoA in eukaryotic host cells at Tyr-34, using UDP-N-acetylglucosamine (UDP-GlcNAc) as the sugar donor. Glycosylation of RhoA results in impaired effector and regulator interaction and inactivation of downstream RhoA signaling which leads to actin filament depolymerization and blocks cytokinesis and gastrulation during zebrafish embryo development. To a lesser extent, is also able to glycosylate other Rho family GTPases (RhoB, RhoC, Rac1, Rac2, Rac3, and Cdc42) in vitro at a switch I tyrosine residue, but not Ras proteins.
Subcellular location: Secreted {ECO:0000305|PubMed:26190758}. Host cell membrane {ECO:0000269|PubMed:26190758}. Note=Is likely secreted by the antifeeding prophage tail (Afp) protein translocation system into eukaryotic host cell. Targets the host cell membrane, and shows co-localization with RhoA. {ECO:0000269|PubMed:26190758}.
Interaction partners:
Sequence:
MPYFNKSKKNEIRPEKSKEEVGGVLFDDSAIHENIDHNMEPQTGDSVATFPDNSDEVVGGDLAALRARLQATIGDYLPEFYEFQQTGRNILYPKPVDIKALQARLNSMPITAWIQPLQQQLEQAGNAFLDRFRLFRKEKDWHNNALEFVKFRLAVKETGWDEQQTRDYNEALKHANRLSGYLSGTLDLIQHLNDNPERQTSWEAVRKTSYDMLLNELLPVDAENNGNQYTAKIRGNLTKNHPDNIKFNTIVNSVTRDERMASEALSAETKGLSARLLSGIALTRNSAEKIDKKSETSGITRDIVALCQVLQGVIKTIKRRGEYIQPLTDWQPSEHKLPGQKEELTKAETVKRELVKSRKVMMQKVQGAKTILGVLSDKTNKNRHRITQTLPYSANPDTNTVSRKTNEAVFRAGIMLLDKIQQTTSGIHKASLASRPLQHAVTQYSALEQMSSGMPSNRILDAKLRSESGRWQEKAEKSKKQLQHILREITALAEEHLKHKFMSALRDELKRAPETLASNNIISNFDTRAKIVVEGLASIEIGMNQSVVRLAGHGEAGRKDLDEQVVAWLQRLERIKGELKTDITQATGQSINNFSRQGMLARRMGEWNEAEKQRYLAALSTEDRAVAEMQYNTLFFEVIQHYLPLLSKETDPQGERLLQRLRLEVSNAAEGTTVYPATMAEILAGMKSTEQAIRDWSGRKLLRVVFLAACLEGVKLMPKLAALPLRVAIKFVITGAKVAWATHKGQQGIRGGEGDVDDEIGEYAKRSFKTASVKVVLSLPPGLATMLGVASIALDVYEGGLKGAGGKIAKNIVGDAPWRALNQGSKIAAEAYTTASMNAALKEGGANPVSHSSTLQQQMDAKPFFDNSDQDADQPRVRRKREMTDEIMLSDGSSRSEAKALPDENELDTDQSKSRPESALAVETLQSEHFDFDRGIRYQDFSDEQKKQTYLHGIKFVLLQIENDGHFAQNIRNNAYLARIGAKLAVPVDIYRYKLNNTFLLPDEIDSKSGVLIRLDSEIPYYYVSEGKDLLENIAWAMPYNAANRGPLKFSLDPGEVTSSHSGVDILNNIRSERFKFETYFNYNAPEAMSIESLSAQLANTIEADYKFKNTSPTNKILISRAIVGAHIPDPGVRATQGEYHIEFDSDELAPAKYLRSFARPFSTLSGEMQLISSSIKGETIQETELHVHQAEYIGSWVDATAGAIISFTPEGWFLNTAQSAAEITADLTEGKDPDPLAVAGLLVGIIPGGKIAAKVGKFTRIGGKTVKYGLILGNKSVDLAIVGKSIKTAVDTGEPLAIYQAFLASGMSVKNSYDIAKNMSSELKISKKIEESARLRKLKALQKNTYKYSMNSKMPVRKFRVGQTDLLGKIHKGEIKISRNNGTTWEKGNQLHLLAYRLQNAGGGRLLPDVFRDKIVIGEYSFKRVKYNQKKLNEMMRIAKMYTPTSNSTERIAKLQQNYKTGKEMSHAPQYDTYNDLSLGEKLDLFINSNTDATTRGVLAGKINESITNINLYETAKGVDAWKTSANKATDVVLAPQNIFLKGRAGECLPESILMGWALQSGQDTKLAKKLMNIHSSSNIAANPLYKSLVELHSDGNASRFGESVISDINMKMLSGAESKLFPTENSSVRVDIPEHTMLISKVNKDGKIKYVFYDPNYGMAYFNKYKEMISFFKKKIKGYDTPKRSTSFRQLDYSHLSDIKIKGKNLNEIIDGEIPQIFRQEDVNLEGITPQDGIYRMLGTHQQENNTYIKSQNNIYQVEWDQTTNTWRVFDSANTNRSRITVPIKRDTDGEWFKYTETGLKGGGLFDEIKNNWLQRKRFKNLQDFNDIVDFEENKWPSEPINKDIHMIWVGTRNISEKNIGLSLETARKNSDYNTTIIYDSGIAGYESAKDFMTEKFKDSKVTLVDFRKKSYFHQLQQEPSFPYYEQAIRDKKYAQASDILRLLVLKYEGGIYKDIDDVQVKAFGSLAFPKGIGVMREYAPEAGKTTAFPNTPIAATKNNPVVNKTLELAVENYHRGETNVLKLAGPDVFTESLYQEIPGMRPQVLGAQLDQFELAKRQALGMRLEKPKGFADEKLTLQEKAKIRQPYEAIRGLSGYVDNGADHSWVTDMPGNSTQSSGLS