Protein: Vacuolar protein sorting-associated protein 18 homolog (Q23194)
Organism: Caenorhabditis elegans
Function: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the rab-5-to-rab-7 endosome conversion probably implicating sand-1, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion (By similarity). The HOPS complex is proposed to be recruited to rab-7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes (By similarity). Within the HOPS complex, contributes to the normal development of gut granules in intestinal cells of the embryo, and also promotes the trafficking of embryonic intestinal gut granules away from lysosomes. The CORVET complex is proposed to function as a rab-5 effector to mediate early endosome fusion probably in specific endosome subpopulations (By similarity). Required for fusion of endosomes and autophagosomes with lysosomes. Plays a role in the degradation of apoptotic cells during programmed cell death.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:18923146}. Late endosome membrane {ECO:0000250|UniProtKB:Q9P253}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9P253}; Cytoplasmic side {ECO:0000250|UniProtKB:Q9P253}. Lysosome membrane {ECO:0000250|UniProtKB:Q9P253}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9P253}; Cytoplasmic side {ECO:0000250|UniProtKB:Q9P253}. Early endosome {ECO:0000250|UniProtKB:Q9P253}. Cytoplasmic vesicle, autophagosome {ECO:0000250|UniProtKB:Q9P253}. Cytoplasmic vesicle, clathrin-coated vesicle {ECO:0000250|UniProtKB:Q9P253}.
Interaction partners:
- Vacuolar protein sorting-associated protein 39 homolog
- Vps8 domain-containing protein
- Vacuolar protein sorting-associated protein 41 homolog
- Vacuolar protein sorting-associated protein 11 homolog
- Vacuolar protein sorting-associated protein 16 homolog
- Vacuolar protein sorting-associated protein 33A
- Vacuolar fusion protein CCZ1 homolog
- Ras-related protein Rab-7a
- Vacuolar protein sorting-associated protein 33B
- t-SNARE coiled-coil homology domain-containing protein
Sequence:
MIKNTAKNKNGIITRATIDLKKDLKFVKLTAVSNLAVQNGEMLAAVTEKLLVHYSEGTGERHQEMSLPLNGPDHVAYIHLSRTGFHAIVSSKLGHNFYIHLKSNAFHHLKKLRCVVTAVGWNPDYSKETDTTGPILLGTAQGSIIELNVGSTGMMTTLKELTSQVAQIAEQRITSAPSPAAAITDIQLFQLADDDPKNKKWMVIIAQMARLIVLITDNEPAPVVKLGGFTSSASLQAGLMNLATEQAPSTTFHSFFTSPNTLQHTISSSKFSEKFKNHGFLTMHPTIAEPKRYAWLSPDGISIGNVNIYAERIQDVLVEEFNIEHRLIEGRLEPPTGIALTDYHVLLAYSSRVLALSLLPPHDVIFEDPWNPELGGALGFVSDNVAEFVWLYTQTFAMKYGTNDEARYIWKTYLDRGEYQKALQIARTRVAIEPDALEMVLRKQADFYIQEKNFTAAAEILAQSSEPFESVVLKFLTNSSERKMGLKTLLDKKLERLTRHEDKIRRDALVMWLLNVQLEELAEMRRLKNSNPDPAFVEKLRDTTDHVQRYFMRKNVIESIQTNRDAVYRMCVAHADFEMQLFFANAVKDLRTVIDILMLREQYFEVLEVLKNQRISELTYEMCPLLIEHIPKQVIVYLIQNQDQISPQKLTPCLSLCVKNMEMAIPAIKYLEAQFKGTQTISQNPQNLANLHNIYIHLMAKFRREKLLGYLESHGTIRSDLPYELDFAMRTCEQFKIEPCVVYLFCVAGMFGDAVEKALGFDVDLAKKCALMMEEAEANFAWLEGMEDPAATSYIRQKLDEKAKKAIWLKIGQYYVTQENNVDKCIELINESNHLLTIQDLLPIIPKFTRVGALKPIIVDFLKRNKQRLEKLERSMKEATEIASEIRDKQEKLKNRTTVVKPSDVCSHCARPISGRAFNVHSCRHFFHRECLEIAMISFLSQEEVEKMKTLIIDEERVLSQMKAEQLAGNQKGFIEKQEKYLKIAAFISNIVGAECPLCGNIAISQIDKQFLSDEEFAADLNTWLL