Protein: Pre-mRNA-splicing factor brr2 (EC 3.6.4.13) (Pre-mRNA-splicing factor spp41) (Pre-mRNA-splicing helicase BRR2) (Q9UT24)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Involved in pre-mRNA splicing. May be involved in endoplasmic reticulum-associated protein degradation (ERAD) and required for growth at low and high temperatures (By similarity). Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U5. Has a role in branch site-3' splice site selection. Associates with the branch site-3' splice 3'-exon region.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus {ECO:0000269|PubMed:16823372}.
Interaction partners:
- Small nuclear ribonucleoprotein F
- Pre-mRNA-splicing factor cwf14
- Pre-mRNA-splicing factor spp42
- Pre-mRNA-processing protein 45
- U2 small nuclear ribonucleoprotein A'
- Pre-mRNA-processing factor 17
- Pre-mRNA-processing factor 31
- Pre-mRNA-splicing factor syf2
- Pre-mRNA-splicing ATP-dependent RNA helicase prp28
- U4/U6.U5 tri-snRNP-associated protein snu66
Sequence:
MSSAHPKGDSKEPPKHGNSKEKPNYGQSQYSYSAMSNLVTQADRRFVSRRDAEPTGEPESLVNRVSIADMGSRARIEKPSTLPLELTQEVQEVRLPRKDAESLEIGIRQPEREKRSSAILKYFDSFEILKYNPLTDETREVYDYILSFIQQYLGDQSPEILRSAADLIIELLKDSSLDEQGRKKQIEEVLSTELPQDRFSQLVNLGNRLTDYTVEQEEELNEEGVNESGVPVLFNEADEEEEAVEAMEEDEVAEDEDVVLETSISQEEEKKNIENPDTEVTFISADTKKVTEIPTVHPREIDAFWLQREIAKYFADAVVCQEKTNQAFEALSADYDLGELENELMSIFDYEHFYLVQLLTKNRWTIVSCTMLKRAATDEERLGVEEQIRAAGRSWILEALRPGAITIPDDGLNELNNNVVEKAEPAPVSEIPLSKTLTSHKIVPKHQVDLENYVFTEGSRLMSNKAVKLPEGSFRRTGKGYEEIHVPAPNKAVLGADERLVKIKELPEWSHQAFLNTQSLNRIQSHLYPIAFGTDENILLCAPTGAGKTNVAMLCILNELQKHLREDLSFNLQNFKIVYIAPLKALVQEMVNNFSKRLTPYNIRVAELTGDSQLTKQQISETQIIVTTPEKWDIITRKANDLSYVNLVRLVIIDEVHLLHDERGPVLESIVARIFRHQEETLEQVRLVGLSATLPNYTDVASFLHVDPKKGLFYFDSTYRPCPLKQEFIGITEKTPFKRMQTTNEACYEKVMQHAGKNQVLIFVHSRKETAKTARFIRDKALEEETIGHLLRSDAASREILRAEADSTSDENLKDLLPYGFAIHHAGMRREDRQTSEDLFADGTIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGIWTELSPQDVLQMLGRAGRPQFDTYGEGIIITAHSELQYYLSLMNQQLPIESQFMRRLADCLNAEVSLGTVRSIEDGVDWLGYTYLYVRMLRSPALYSVGPEYDDDKYLVQKRADLLHSAAILLEKCKLLVYNRQSGTLTATELGKVAASYYVTHNSMAIYNRLLMQTTSFIELFRVFSFSDEFKHIPVREEEKVELAKLLERVPIPIRERLDEPAAKINALLQSYISRQRLDGFALVADMVYVTQSAGRIMRAIFEISLRRGWSSVATLSLDTCKMIEKRLWPTMSPLRQFPNCPSEVIRRVEKKEFPWQRYFDLDPAELGELVGVPKEGRRVYNMVQSFPRLSVEAHVQPITRSLVRVELVINSQFNWDDHLSGTSEAFWILVEDVDGDRLLHYEQFFLLKKYKDDEHIVNFTVPLLEPLPPCYFIKIVSDRWLHSITKVPLSFQRLIMPEKFPAPTPLLDLQNAPVSSLNNPSFISLYPNFKFFNKIQTQVFNSVYKTNDSVFIGAPNGSGKTVCAELALLHHWSQEDYGTAVYIAPIQEIVDRRYEEWYGKFSDLGDGKVLVKLTGERSQDLKLIQVADLIFCTPSQWDSLSKRWRSMRSIQKVDFYICDELQLLGGFYGPLYEIVISRIRYMAVQLEKNIRVVGLSVSVANARDLGEWLGTSPQCIFNFSPKDRPNPLTIHLQSFSITHFPSLMLAMSKPIYRSLKNFISQRKSTIVFTPDRKVAKQLAFDLVTFSMADEDEYLFSLMENEAFNKVEDAALQQSLKHGIAYISEITSSNDQNIVQYLYRHGLIKVLIASRDVIYSLKAKSNAVIVMGTQYYDGKEHRYIDYPISELLQMLGFTASIGSSELSQVILMTVTTKKEYYKKFLNEPLPMESHLQVWLHDAFVSEISTQTIESKQDAVDWLTWSYMYRRLVANPAYYGLQDITHESVSEFLSDLVETTMNDLSEARLITVDDEDDSCVALNLAMIASHYGITYITMQTFALSLSERTKMKGLLEIVTSAAEYEQLPIRKYEDIVLERIHSRLPVRLSNPNYEDPHTKSFILLAAHFSRFELPPGLVIDQKFILTRVHNLLGACVDTLSSEGHLIACIRPMEMSQMVTQALWDRDSPLKQIPYFDDALIERCNKEGVHDVFDIIDLDDEKRTELLHMDNAHLAKCAEFINKYPDIDIDFEIEDSEDVHANSPSVLIVQLTRELEEDEEVDTTVIAPYFPAQKTEHWWLVISDDKTLLAIKKITLGRSLTTKMEFVPPAMGTLKYKLSCFSDSYMGVDYEKEFECNVLEPLDTEMEDGE