Protein: Rapamycin-insensitive companion of mTOR (AVO3 homolog) (mAVO3) (Protein pianissimo) (Q6QI06)
Organism: Mus musculus (Mouse)
Function: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
Subcellular location: Cell membrane {ECO:0000250|UniProtKB:Q6R327}. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q6R327}. Lysosome membrane {ECO:0000250|UniProtKB:Q6R327}. Note=The mTORC2 complex localizes to membranes: mTORC2 is active at the plasma membrane, endoplasmic reticulum membrane and lysosomes. Iin lysosomal membrane, mTORC2 is sensitive to lysosomal positioning in the cell. {ECO:0000250|UniProtKB:Q6R327}.
Interaction partners:
- Serine/threonine-protein kinase mTOR
- Regulatory-associated protein of mTOR
- Proline-rich protein 5
- Target of rapamycin complex 2 subunit MAPKAP1
- Target of rapamycin complex subunit LST8
- TELO2-interacting protein 1 homolog
- Proline-rich AKT1 substrate 1
- DEP domain-containing mTOR-interacting protein
- Telomere length regulation protein TEL2 homolog
- Proline-rich protein 5-like
Sequence:
MAAIGRGRSLKNLRIRGRNDSGEENVPLDLTREPSDNLREILQNVAKLQGVSNMRKLGHLNNFTKLLCDIGHSEEKLGFNYEDIIICLRLALLNEAKEVRAAGLRALRYLIQDSSILQKVLKLKVDYLIARCIDIQQSNEVERTQALRLVRKMITVNASLFPSSVANSLIAVGNDGLQERDRMVRACIAIICELALQNPEVVALRGGLNTILKNVIDCQLSRINEALITTILHLLNHPKTRQYVRADVELERILAPYTDFHYRHSPDTAEGQLKEDREARFLASKMGIIATFRSWAGIINLCKPGNSGIQSLIGVLCIPNMEIRRGLLEVLYDIFRLPLPVVTDEFIEALLSVDPGRFQDSWRLSDGFVAAEAKTILPHRARSRPDLMDNYLALILSAFIRNGLLEGLVEVITNSDDHISVRATILLGELLHMANTILPHSHSHHLHCLPTLMNMAASFDIPKEKRLRASAALNCLNRFHEMKKRGPKPYSLHLDHIIQKAIATHHKRDQYLRVQKDIFVLKDTEEALLINLRDSQVLQHKENLDWDWNLIGTILKWPNVNLRNYKDEQLHRFVRRLLYFYKPSSKLYASLDLDLAKSKQLTVVGCQFTEFLLESEEDGQGYLEDLVKDIVQWLNASSGVKPERSLQNNGLLTTLSQHYFLFIGTLSCHPHGVKMLEKCSVFQCLLNLCSLKNQDHLIKLTVSSLDYSRDGLARVILSKILTAATDACRLYATKHLRVLLRANVEFFNNWGIELLVTQLHDKNKTISSEALDILDEACEDKANLHALIQMKPALSHLGDKGLLLLLRFLSIPKGFSYLNERGYVAKQLEKWHKEYNSKYVDLIEEQLNEALTTYRKPIDGDNYVRRSNQRLQRPHVYLPVHLYGQLVHHKTGCHLLEVQSIITELCHNVRTPDLDKWEDIKKLKASLWALGNIGSSNWGLNLLQEENVIPDILKLAKQCEVLSIRGTCVYVLGLIAKTKQGCDILKCHSWDSVRHSRKHLWPVVPDDVEQLCNELSSVPSTLSLNSESTSSRHNSESESAPSSMFMLEDDRFGSTSTSTFFLDINEDAEPAFYDRPGPIKDKNSFPFFGSSKLVKNRILNSLTLPTKKHRSSSDPKGGKLSSENKTSNRRIRTLTEPSVDLNHSEDFTSSSAQKSLQLEPSFVGNKHLEDAGSTPSIGENDLKFPKSFGTETHRENTSRERLVVEGSASSHIKIRSQSFNTDTTTSGISSMSSSPSRETVAVDPTAMDTDCGSLSTVVSTKTVKTSHYLTPQSNHLSLSKSNSVSLVPPGSSHTLPRRAQSLKAPSIATIKSLADCNFSYTSSRDAFGYATLKRLQQQRMHPSLSHSEALASPAKDVLFTDTITMKANSFESRLTPSRFMKALSYASLDKEDLLSPINHNTLQRSSSVRSMVSSATYGGSDDYIGLALPVDINDIFQIKDVPYFQSKHVPPPDDRGARMFSHDGAGLSSGAGGLVKNSFHLLRQQMSLTEIMNSVHSDASLFLESTEDTGLQEHTDDNCLYCVCIELLGFQPSNQLSSICSHSDLQDIPYSDWCEQTIHNPLEVVPSKFSGISGCSDGASQEEGSASSTKSTELLLGVKTIPDDTPMCRILLRKEVLRLVVNLSSSVSTKCHETGLLTIKEKYPQTFDDICLYSEVSHLLSHCTFRLQCRRFIQELFQDVQFLQMHEEAEAVLAIPPIQPIVDESAES