Protein: Chromodomain-helicase-DNA-binding protein 1 (CHD-1) (EC 3.6.4.12) (ATP-dependent helicase CHD1) (P40201)
Organism: Mus musculus (Mouse)
Function: ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3 (By similarity). Required for maintaining open chromatin and pluripotency in embryonic stem cells. Is also associated with histone deacetylase (HDAC) activity.
Subcellular location: Nucleus {ECO:0000269|PubMed:10199952, ECO:0000269|PubMed:7739555}. Cytoplasm {ECO:0000269|PubMed:7739555}. Note=Is released into the cytoplasm when cells enter mitosis and is reincorporated into chromatin during telophase-cytokinesis (PubMed:7739555).
Interaction partners:
- RNA polymerase-associated protein RTF1 homolog
- Histone H3.3
- Histone H3.2
- Transcription elongation factor SPT5
- Bromodomain PHD finger transcription factor
- Histone H3.1
- Histone H2A.Z
- FACT complex subunit SPT16
- DNA helicase
- Lysine-specific histone demethylase 1A
Sequence:
MNGHSDEESVRNGSGESSQSGDDCGSASGSGSGSSSGSSSDGSSSQSGSSDSDSGSDSGSQSESESDTSRENKVQAKPPKVDGAEFWKSSPSILAVQRSAMLRKQPQQAQQQRPASSNSGSEEDSSSSEDSDDSSSGAKRKKHNDEDWQMSGSGSPSQSGSDSESEEERDKSSCDGTESDYEPKNKVRSRKPQNRSKSKNGKKILGQKKRQIDSSEDEDDEDYDNDKRSSRRQATVNVSYKEDEEMKTDSDDLLEVCGEDVPQPEDEEFETIERVMDCRVGRKGATGATTTIYAVEADGDPNAGFERNKEPGDIQYLIKWKGWSHIHNTWETEETLKQQNVRGMKKLDNYKKKDQETKRWLKNASPEDVEYYNCQQELTDDLHKQYQIVERIIAHSNQKSAAGLPDYYCKWQGLPYSECSWEDGALISKKFQTCIDEYFSRNQSKTTPFKDCKVLKQRPRFVALKKQPSYIGGHEGLELRDYQLNGLNWLAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQTWASQMNAVVYLGDINSRNMIRTHEWMHPQTKRLKFNILLTTYEILLKDKAFLGGLNWAFIGVDEAHRLKNDDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFSSWEDFEEEHGKGREYGYASLHKELEPFLLRRVKKDVEKSLPAKVEQILRMEMSALQKQYYKWILTRNYKALSKGSKGSTSGFLNIMMELKKCCNHCYLIKPPDNNEFYNKQEALQHLIRSSGKLILLDKLLIRLRERGNRVLIFSQMVRMLDILAEYLKYRQFPFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEDILERAKKKMVLDHLVIQRMDTTGKTVLHTGSAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETHENEPGPLSVGDELLSQFKVANFSNMDEDDIELEPERNSKNWEEIIPEEQRRRLEEEERQKELEEIYMLPRMRNCAKQISFNGSEGRRSRSRRYSGSDSDSISERKRPKKRGRPRTIPRENIKGFSDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSETDLRRLGELVHNGCVKALKDSSSGTERAGGRLGKVKGPTFRISGVQVNAKLVIAHEDELIPLHKSIPSDPEERKQYTIPCHTKAAHFDIDWGKEDDSNLLIGIYEYGYGSWEMIKMDPDLSLTHKILPDDPDKKPQAKQLQTRADYLIKLLSRDLAKREAQRLCGAGGSKRRKTRAKKSKAMKSIKVKEEIKSDSSPLPSEKSDEDDDKLNDSKPESKDRSKKSVVSDAPVHITASGEPVPIAEESEELDQKTFSICKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYSNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQESQQNSDQNSNVATTHVIRNPDMERLKENTNHDDSSRDSYSSDRHLSQYHDHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHREWDHYRQDSRYYSDREKHRKLDDHRSREHRPSLEGGLKDRCHSDHRSHSDHRMHSDHRSSSEHTHHKSSRDYRYLSDWQLDHRAASSGPRSPLDQRSPYGSRSPFEHSAEHRSTPEHTWSSRKT