Protein: Inner centromere protein (Q9WU62)
Organism: Mus musculus (Mouse)
Function: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus associated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules. The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin (By similarity). Activates AURKB and AURKC. Controls the kinetochore localization of BUB1.
Subcellular location: Chromosome, centromere {ECO:0000269|PubMed:12584241}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:12584241}. Nucleus {ECO:0000269|PubMed:12584241}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:12584241}. Midbody {ECO:0000250|UniProtKB:Q9NQS7}. Note=Localizes to inner kinetochore. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes to the equatorial cell cortex at anaphase (By similarity). Localized at synaptonemal complex central element from zygotene up to late pachytene when it begins to relocalize to heterochromatic chromocenters (PubMed:12584241). Colocalizes with AURKB at a connecting strand traversing the centromere region and joining sister kinetochores, in metaphase II centromeres. This strand disappears at the metaphase II/anaphase II transition and relocalizes to the spindle midzone (PubMed:12584241). {ECO:0000250|UniProtKB:Q9NQS7, ECO:0000269|PubMed:12584241}.
Interaction partners:
- Baculoviral IAP repeat-containing protein 5
- Aurora kinase B
- Borealin
- Aurora kinase A
- Aurora kinase C
- Serine/threonine-protein kinase PLK1
- Kinesin-like protein KIF23
- Kinesin-like protein KIF20A
- Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
- Cell division cycle protein 20 homolog
Sequence:
MGTTAPGPICLLDLCDQKLLDFVCNVDNKDFMWLKEIEEEAERMFIREFSNEPELMPKTPSQKNRRKKRRVSNIQDENRDPVRKRLSRRKSRSSQVGTRHLRSKPVTIVEENGFPVLQRITRATAAAAAAAAAASVASASSSSTAGSPTVLTKKAVVEISTSERLSAELQLTKLKGSLPPSPVSQGTLTSEEELTPKKSEAGKLDSVTVNSLKATPQSPKNRGVGEGRSVSKLKIARASWGLQDSPGSTDSPWQERVLSPILLNNILPTTAKSPLGNIRSVRRSLISQDSQVPLASKYNLVAKQENGSRRSSRRIAKKAGKEPEASARIICHSYLERLLNVEVPQNVGLEQEPVEVAEPEEAEEEQEVSKNSGCPSKPRSATKIAISTPTSKPAAAGQTTTVEEQEAELDQTDGHREPPQSVRRKRSYKQAISEPDEEQLEDEELQPCQNKTPSPPCPANKVVRPLRTFLHTVQKNQMLMTPTLASRSSVMKSFIKRNTPLRVDPKCSFVEKERQRLESLRRKEEAEQRRRQKVEEDKRRRLEEVKLKREERLRKVLQARERVEQMKEEKKKQIEQKFAQIDEKTEKAKEERLAEKAKKKATAKKMEEVEARRKQEEEARRLRWLQQEEEERRHQEMLQRKKEEEQERRKAAEARRLAEQREQERRREQERREQERREQERREQERKEQERREQEQERLRAKREMQEREKALRLQKERLQKELEEKKRKEEQQRLAEQQLQEEQAKKAKEVAAARKVLNMTVDVQSPVCTSYQMTPQGPKSIPKISVDDYGMDLNSDDSTDDESHPRKPIPSWAKGTQLSQAIVHQYYHPPNILELFGSILPLDLEDIFKKRKTRYHKRTSSAVWNSPPLKATMVPSSGD