Protein: Midasin (Dynein-related AAA-ATPase mdn1) (MIDAS-containing protein) (O94248)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Nuclear chaperone required for maturation and nuclear export of pre-60S ribosome subunits. Functions at successive maturation steps to remove ribosomal factors at critical transition points, first driving the exit of early pre-60S particles from the nucleolus and then driving late pre-60S particles from the nucleus.
Subcellular location: Nucleus {ECO:0000269|PubMed:16823372}. Nucleus, nucleolus {ECO:0000250|UniProtKB:Q12019}. Nucleus, nucleoplasm {ECO:0000250|UniProtKB:Q12019}.
Interaction partners:
- Ribosome assembly protein 4
- Ribosome biogenesis protein ytm1
- Pre-rRNA-processing protein crb3/ipi3
- Nucleolar GTP-binding protein 2
- Ribosome biogenesis protein nsa1
- Protein sda1
- Ribosome biogenesis protein rlp24
- GTPase grn1
- Ribosome biogenesis protein nsa2
- Ribosome assembly factor mrt4
Sequence:
MDVLIEWVAIYPQIYDILEHINYVPSNTLQRLRLHQPWSKIDYDVWFLYASDEIRETCKVKYYGETKTYGEVFVLENERISQLHRLFVSWTVSERAEHLKNLLFDAGLSNLPLVELGGNVFFNSHVPLPCSLVLTKSTQENLNRITPYLVQKRPILLAGPEGIGKKFLITQIAAKLGQQIIRIHLSDSTDPKMLIGTYTSPKPGEFEWQPGVLTQAVITGKWILFTNIEHAPSEVLSVLLPLLEKRQLVIPSRGETIYAKGSFQMFATSSMKTKILGQRLWQILDLTYQPDECVEVVSTLYPVLSIICPTLYSVYKDIFDLFSQRSFLATSKIYRRLCLRDFYKFIKRVAFLYHKFMIPSDHVVISQELQDAVFKEAIDMFGAFIPSRDGFDLVVRNVAIELNIPPEKALQLRYSIPVFQNLEHNINIGRCSLKKLSTIRSCSTNSYAFTSSSLGLLEQLAAGVQTNEPLLLVGETGTGKTTTIQLLAGLLGQKVTVINMSQQTESSDMLGGYKPINASTLGLPLHERFIDIFEQTFSSKKNAKFISMASTSARRFRWKTCLKIWKEACKLSKTVLDGQQPLPNPQKRQKRLSNQVELRNQWAKFEKEVEDFEKVLTGGSNGFMFSFVEGALVKAVRSGHWVLLDEINLASLETLEPIGQLLSSYESGILLSERGDITPITPHKNFRLFGCMNPSTDVGKRELEPSFRSRFTEIYVHSPDQNLDDLLSIIQKYIGSLCIGNEHVIREVAELYQVAKSLSLDGSLVDGAGQRPHYTVRTLSRTLSYVTEIAPIYGLRRSLYEGFCMSFLTLLDHTSESLLYNHVVRFTLGELNRDQQNAILKQIPKVPDHSSYIAFCHYWLRRGSFPVEEQEHYIITPFVQKNLLNIARACSTRMFPILIQGPTSSGKTSMIEYVAKKTGHKFVRINNHEHTDLQEYIGTYVTDDNGSLSFREGVLVEALRNGYWIVLDELNLAPTDVLEALNRLLDDNRELFIPETQVLVKPHPEFMLFATQNPPGVYAGRKHLSRAFRNRFLEIHFDDIPENELETILHKRCKIAPSYAAKIVQVFRELSLRRQTTRIFEQKNSFATLRDLFRWAFREAVGYQQLAENGYMLLAERARDQKDKLAVQEVIEKVMKVKIDTDGIYNLDSMEIFQDMSLKEGPLSKVVWTRPMIRLFCLVWRCLLAKEPVLLVGDTGCGKTTVCQILAECLHKELHIINAHQDTENGDIIGAQRPVRNRSAVNYSLHSQLCEKFNVQESLDSIDDLIEKFEKLSSSEKNDNLSNLIERQIIKYRSLFEWHDGALVTAMKQGDFFLLDEISLADDSVLERLNSVLELSRTLTLVEHSNAAVSLTAKDGFAFFATMNPGGDYGKKELSPALRNRFTEIWVPPMVDTEDILKIVEGKLHNNKIELARPLVEYAKWHANEYLYTDVISIRDVLSAVEFINACEILDLNLVLFNAVSMVFIDALGSFTTFSLSNNLASLHAERQRCFAKLNELAGSNIMASKSADISIKFSDSSFFIGDFGIPLGDSVESDSTYSLHTDTTLMNASKVLRALQVLKPILLEGSPGVGKTSLITALARETGHQLVRINLSDQTDLMDLFGSDVPVEGGEGGQFAWRDAPFLAAMRNGHWVLLDELNLASQSVLEGLNACLDHRNEAYIPELDKVFKAHPNFRVFAAQNPQHQGGGRKGLPRSFINRFSVVYVEALKEKDMIEIAACNYHQVNEDWRLKIIKFMFRLQDNIEKDISFGSFGSPWEFNLRDTLRWLQLLNDAPKYTCVSPADYLEVMVLHRMRTVEDRVRTCELFKEVFDIDYEPRTIGFSLSSQCFKVGHSLLVRDVERQKTLLDSQNILQSQLPVLESVITCINKKWPCILVGDTATGKTCILRLLAAIAGAKIKEMAVNSDTDTMDLIGEYEQIDISRKASELFTDLSQQLLNIVIKYRNFDNIFRETSLYTLTTTSFKTHSQAFTLLQKVVDQLDQLKIHETLVHSLGDIHEKARKLLAEFSASPAGRFEWFDGYLLKAVEEGHWFVLDNANLCSPAVLDRLNSLLEHKGVLIVNEKTTEDGHPKTIKPHPNFRLFLTVNPVYGELSRAMRNRGVEIFLLKEALTEIDKKQMSLLEPAPISSAVDTLASNISYIKYVFETMGKIEIDGNYMYIAHAIILALFSPRQLKLLRKVLLTNPQFSLSIKADAELLLTLKNLVQKIYCADYFNHMDLKASRFMDIYEYPVQLREVVGLIQTINDFQSVILTSHLELPETYASGLLFVSAHEILDLTEEVNRLAVSTSNSTYLLKSASAVYHNVSSFKGSTPSLWNLLNQFSKFLIEIASANSNIVYKLSYDVIRHFLKLVVLWKNIYVWTNVPDCDISRFYCYTKMLGEWMFTLTEKTKLLESFLPKDSLEKFSELQNLSTGLHMQAIWDKWHAFVPRTYDQWSLWNTVDKLLTQYVNANIPSISMETTACEVVGTSLSLLNKVLVENEVGDIYSYLKILGKGVNELKSSKQVILPENLVNLFNCLASLDLLHIFIKYTTSSFFLTDDFVRFIRVCFHSRISGNLLTLLHGISFDSTKAVAPVLTYFDFCSLTTGNILGRIALAFTSIDENANLESANIFEHARLALLQHFMDHSSLLAEDSSTKMNLILLQRYAVIISIFLDQGKCEKANDLITKLSLPYEELAENFVSILEACKAFLVANSEFISYTYTERFIHSLRFLKDSWLSSNQQKMLKNQGMAYIYFASGMLLVYVPDKPFDPALLPLLTVESLRHYLESLYKESQILEIAESLNSGKVNSVMRRLVSTEISNTPNIDSSFSTVYRSLNESIVPLYSELEFFMKSVVLNQYIFELAMRLSKESNIAVVEEAKSFVTKWKAYIERIREAYPQFVDVYELILSFISFMIYGIELLMFEAKRRLDERSQILSTLILTLVDPSSFARSLSFDDVSNLIEQIKVLDLNDSIRFEIYLFLASRLCSEKQHSSDTHSLANSFVLLANEFYIHNAKIKQKELEEIEEKNRLYRQREFNFDKNDYLKVFINYDDEVEPEVEPEVVIERKRFLQLQFAFWSLYNEIYSEKMNVIPLEQLMNTGSYLAKKIKVKNPDMIASSGFDIVSVVLMMGVKSTNERQYWTPPVYNFYSDPNPSKAIEVRDLIKIVESRAISLIKNWPENFVLRGLKDAIDAILNLSPFSPIAEYLSKLERVFHLLSEWEKLASREYSLANEMDLIKKKIIDWRKFELSNWNNLLKLEEYKLSERVYPRLYSILQFIILKPFFENSKFTKQNLCESASIIVQFITDLTVGEFQLCLKCLLSFSQHAASLRICHGIDAMLLNIYHYFEQFLSKVSEAIHTQKQSLENSIKERILLMSWKDTNVYALKESAKKSHAELFKVLHRYREVLRQPVSSYLSQKHDWDSLLDTENNSAMWVAKKVNLSPSYIEKMDTEIMKLVPVRFSNTPTTLRLMWTLFANVEKPGSTFTNMVSNLITDARELMKLTPETINDDNLSEIKHLKSRKHLLLTETFKTLKAFGLQYRVKAGIEENLSNLRNLLAVIPTFPVTSLSIEKVDRSLMKSLDFIPKFQTLAGHQHNDLSVPEVQKGVGLFNSMLSLQLGERAQLVEFTNELLALKNVYSEVGVNGSPLESFNNSSFNEVSSLGYDHDFENRAQAVSMLCQIYAIVIQKHSSISPTASFQSIGHELSRFADLLSNKLFPSSIPLYASADKVSSIRDQQKGINDLIEYCRKKRTELPELSYCFKHLVSLQSLKSISRTQVDLTNDEFLNLMNFVLNLFDSLLSSIETATKNMRTFKELAETSSFIEMSSCFSKVLRAFNLKFQSMKLSSLKEKLRSSSVDKMSCQLLMLFLPVCEQFINLAESVLDYFINVHNSNLDSLSKISTLFFMVANNGFCSPDLPQEGKSNSGELESGTGLGSGVGAEDITNTLNEDDDLEELANEEDTANQSDLDESEARELESDMNGVTKDSVVSENENSDSEEENQDLDEEVNDIPEDLSNSLNEKLWDEPNEEDLLETEQKSNEQSAANNESDLVSKEDDNKALEDKDRQEKEDEEEMSDDVGIDDEIQPDIQENNSQPPPENEDHLDLPEDLKLDEKEGDVSKDSDLEDMDMEAADENKEEADAEKDEPMQDFEDPLEENNTLDEDIQQDDFSDLAEDDEKMNEDGFEENVQENEESTEDGVKSDEELEQGEVPEDQAIDNHPKMDAKSTFASAEADEENTDKGIVGENEELGEEDGAAESGVRGNGTADGEFSSAEQVQKGEDTSTPKEAMSEADRQYQSLGDHLREWQQANRIHEWEDLTESQSQAFDDSEFMHVKEDEEEDLQALGNAEKDQIKSIDRDESANQNPDSMNSTNIAEDEADEVGDKQLQDGQDISDIKQTGEDTLPTEFGSINQSEKVFELSEDEDIEDELPDYNVKITNLPAAMPIDEARDLWNKHEDSTKQLSIELCEQLRLILEPTLATKMQGDFRTGKRLNMKRIIPYIASQFKKDKIWMRRVKPSKRTYQVMISIDDSKSMSESGSTVLALETLALVTKALSLLEVGQIAVMKFGEQPELLHPFDKQFSSESGVQMFSHFTFEQSNTNVLALADASMKCFNYANTASHHRSNSDIRQLEIIISDGICEDHDSIRKLLRRAQEEKVMIVFVILDNVNTQKKSSILDIKKVYYDTKEDGTMDLKIQPYIDEFAFDYYLVVRNIEELPQLLSSALRQWFQQMSNT