Protein: Tyrosine-protein phosphatase cdc-14 (EC 3.1.3.48) (Cell division cycle-related protein 14) (P81299)
Organism: Caenorhabditis elegans
Function: Protein phosphatase that negatively regulates the G1-to-S phase transition to inhibit the cell cycle and establish quiescence in cells of multiple lineages including vulval, hypodermal and intestinal. Promotes nuclear accumulation and activity of the cyclin-dependent kinase inhibitor cki-1 which leads to inhibition of G1 progression during vulval tissue development. Has been shown to not be required for cytokinesis. However, in the embryo, in a contrasting study, has been shown to act as a regulator of central spindle formation and cytokinesis, and may be required for localization of the spindle component zen-4, and its interacting partner air-2 at the spindle during late cell divisions.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:12213836, ECO:0000269|PubMed:15247923}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:15247923}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:12213836, ECO:0000269|PubMed:15247923}. Midbody {ECO:0000269|PubMed:12213836}. Nucleus {ECO:0000269|PubMed:15247923}. Note=Localizes to the cytoplasm throughout interphase (PubMed:15247923). As cells enter prophase, accumulates at the centrosomes (PubMed:15247923). During metaphase and anaphase, localizes to the spindle, in particular spindle asters and the spindle mid zone (PubMed:12213836, PubMed:15247923). During late telophase, localizes to cytoplasmic aggregates (PubMed:15247923). In some studies, localizes to the midbody during late telophase (PubMed:12213836). Localizes to the cytoplasm in G1-arrested cells (PubMed:15247923). Localizes to the nucleus or nucleolus in postmitotic cells (PubMed:15247923). Some studies report no localization to centrosomes or nuclei (PubMed:12213836). Co-localizes with zen-4 at the spindle, and localization may be dependent on each other (PubMed:12213836). {ECO:0000269|PubMed:12213836, ECO:0000269|PubMed:15247923}.; SUBCELLULAR LOCATION: [Isoform c]: Cytoplasm {ECO:0000269|PubMed:21723944}. Nucleus {ECO:0000269|PubMed:21723944}. Note=Localizes to the cytoplasm during interphase, but localizes to the nucleus during cell cycle arrest. {ECO:0000269|PubMed:21723944}.
Interaction partners:
- Serine/threonine-protein kinase plk-1
- Serine/threonine-protein kinase sax-1
- Cyclin-dependent kinase 1
- Fizzy-related protein homolog
- Serine/threonine-protein kinase plk-3
- Serine/threonine-protein kinase plk-2
- Separin homolog sep-1
- separase
- Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase wee-1.3
- G2/mitotic-specific cyclin-B
Sequence:
MREDHSPRRNNTIENTLTELLPNRLYFGCFPNPDAIDKSDKSVKKTCFININNKFHYEPFYEDFGPWNLSVLYRLCVQVGKLLEVEEKRSRRVVLFCQDDGTGQYDKIRVNTAYVLGAYLIIYQGFSADDAYLKVSSGETVKFVGFRDASMGSPQYLLHLHDVLRGIEKALKFGWLDFSDFDYEEYEFYERVENGDFNWIIPGKILSFCGPHNESREENGYPYHAPDVYFDYFRENKVSTIVRLNAKNYDASKFTKAGFDHVDLFFIDGSTPSDEIMLKFIKVVDNTKGGVAVHCKAGLGRTGTLIACWMMKEYGLTAGECMGWLRVCRPGSVIGPQQPYLIEKQKFCWSLSQSNGVHLTQNKEEKRNVRRLVNQVDDINLGEERISPKSRENTRPNILRRRVQVQNGRSTAPVTIAPAGTSESRRSTKPSRVVDETALDDQGRSQGDRLLQLKAKHQHESETTSPNSSSSRRFVKSSTPQMTVPSQAYLNRNREPIIVTPSKNGTSSGTSSRQLKTTPNGNVAYRTRNSSGNTTSTLTRTGNESYRFHNSLFYEPASAVFPSMASRRSETTRYLSPTTPIKPMSPSYTDGTSPRYKARLRSENPIGSTTSTPFSLQPQITKCSLTAESKPPKRILSMPGTSKSTSSLKKIQVSRPRPYPSTGVRVELCANGKSYDIRPRKEAHVIPGAGLAANTEALLGVCKLVNTLS