Protein: X-ray repair cross-complementing protein 6 (EC 3.6.4.-) (EC 4.2.99.-) (5'-deoxyribose-5-phosphate lyase Ku70) (5'-dRP/AP lyase Ku70) (ATP-dependent DNA helicase 2 subunit 1) (ATP-dependent DNA helicase II 70 kDa subunit) (CTC box-binding factor 75 kDa subunit) (CTC75) (CTCBF) (DNA repair protein XRCC6) (Ku autoantigen protein p70 homolog) (Ku70) (P23475)
Organism: Mus musculus (Mouse)
Function: Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA (By similarity). Required for double-strand break repair and V(D)J recombination (By similarity). Also has a role in chromosome translocation (By similarity). Has a role in chromosome translocation (By similarity). The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner (By similarity). It works in the 3'-5' direction (By similarity). During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection (By similarity). Binding to DNA may be mediated by XRCC6 (By similarity). The XRCC5-XRRC6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold (By similarity). The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (By similarity). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step (By similarity). Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks (By similarity). 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined (By similarity). The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription (By similarity). In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (By similarity).
Subcellular location: Nucleus {ECO:0000250|UniProtKB:P12956}. Chromosome {ECO:0000250|UniProtKB:P12956}. Cytoplasm {ECO:0000250|UniProtKB:P12956}. Note=When trimethylated, localizes in the cytoplasm. {ECO:0000250|UniProtKB:P12956}.
Interaction partners:
- DNA-dependent protein kinase catalytic subunit
- X-ray repair cross-complementing protein 5
- Non-homologous end-joining factor 1
- DNA repair protein XRCC4
- Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN
- DNA ligase 4
- Serine-protein kinase ATM
- TP53-binding protein 1
- Poly [ADP-ribose] polymerase 1
- Double-strand break repair protein MRE11
Sequence:
MSEWESYYKTEGEEEEEEEESPDTGGEYKYSGRDSLIFLVDASRAMFESQGEDELTPFDMSIQCIQSVYTSKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQDLDNPGAKRVLELDQFKGQQGKKHFRDTVGHGSDYSLSEVLWVCANLFSDVQLKMSHKRIMLFTNEDDPHGRDSAKASRARTKASDLRDTGIFLDLMHLKKPGGFDVSVFYRDIITTAEDEDLGVHFEESSKLEDLLRKVRAKETKKRVLSRLKFKLGEDVVLMVGIYNLVQKANKPFPVRLYRETNEPVKTKTRTFNVNTGSLLLPSDTKRSLTYGTRQIVLEKEETEELKRFDEPGLILMGFKPTVMLKKQHYLRPSLFVYPEESLVSGSSTLFSALLTKCVEKKVIAVCRYTPRKNVSPYFVALVPQEEELDDQNIQVTPGGFQLVFLPYADDKRKVPFTEKVTANQEQIDKMKAIVQKLRFTYRSDSFENPVLQQHFRNLEALALDMMESEQVVDLTLPKVEAIKKRLGSLADEFKELVYPPGYNPEGKVAKRKQDDEGSTSKKPKVELSEEELKAHFRKGTLGKLTVPTLKDICKAHGLKSGPKKQELLDALIRHLEKN