Protein: eIF-2-alpha kinase activator GCN1 (General control non-derepressible protein 1) (Translational activator GCN1) (P33892)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Acts as a positive activator of the GCN2 protein kinase activity in response to low amino acid, carbon, or purine availability. Component of the GCN1-GCN20 complex that forms a complex with GCN2 on translating ribosomes; during this process, GCN1 seems to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of GCN2, and hence stimulating GCN2 kinase activity. Participates in the repression of global protein synthesis and in gene-specific mRNA translation activation, such as the transcriptional activator GCN4, by promoting the GCN2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/SUI2) on 'Ser-52', and hence allowing GCN4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:9234705}.
Interaction partners:
- Protein GCN20
- eIF-2-alpha kinase GCN2
- Phospholipid-transporting ATPase accessory subunit CRF1
- Ribosome-interacting GTPase 2
- Large ribosomal subunit protein eL19A
- Protein GIR2
- Small ribosomal subunit protein uS3
- Small ribosomal subunit protein uS10
- Small ribosomal subunit protein uS15
- Eukaryotic translation initiation factor 2 subunit alpha
Sequence:
MTAILNWEDISPVLEKGTRESHVSKRVPFLQDISQLVRQETLEKPQLSEIAFVLLNTFTIYEDNRSKSLVTSILLDILNLEPCLLENFIRFISDVVISNPATKAVADYLNLLDWINSFLIFVSHNSNLFEEYIPKLLVAHSYATFGVETILDNQEEGKKSQDKQNQHRKRIRYCIFQTTVKAFLKCLKDNDDSISFMKISIKTVLESYSKLKITSVGVVMIMGALTQAALQLLSRQPALHSVLKENSAEKYCEYLGKEVFLGKNPPSSFCLEIGLKPFLKEFVSQELFIKFFIPNIEKAVLRSPEVGFSILSELYAGVSPEKVNLLNAFASSKLINQYFSSFKSSKEVVRSVSLQSMIILLRKISNTDTTLEDLTKLIDEIFKNIKSNLNADYKSLISKILIEIPLTHYEVSEKICKGLSPYIGKEGNEAALTLMLNAFFVHYFSLGKPIEDLDKIISAGFADKKPALKKCWFAAFLNNSNAASEEVILNFIDGCLEFVKDSIIHYQTHGHACILASIEFTNKILALDNTELNDRVMQLIETLPENSSIGDAILTAALSTELSIENRIHAVNLLQELFYKKPEFIGFSVIDAIERRMRVQELIPQQNTSFKYVTSVLLAITSELPDKEASIKVLINALVIAQWNIFNIKNGWAGLVLRARLDPAEVVKEHASVIMEKILEITGSCEWIDTIYGACGLQAAAYAAFIQPNEFTPILCKTIEADLTADDFSRLSEEDFEIFAGEEGVLVVDVLEESMNKKLSNKNSKEYETLMWEQKIRKEQAKKNVKKLSKEEQELVNEQLAKESAVRSHVSEISTRLKRGIRLVSELSKAACLVQNGIATWFPLAVTKLLYLCSEPNISKLTEDVNNVFLQLSQNVSERLGNIRLFLGLATLRVHNANGISQDYLQEPLVELLTRVLFRIKFVSNQAAIDSISLTYILPLLINVLEKGKAIALKNADKPVVKAEFVEEDEEEEHLLLAMEIISVHAEAFEDPSIPRISIVEVLLSLLSLPSKAKIAKDCFNALCQSISVAPNQEDLDMILSNLLSPNQFVRSTILETLDNEFELEPFMKYSPEVFICRFDSDPSNREIADFIWEFNKFVVNDELLKSLFPLFNQDDSGLRLFAANAYAFGAVSLFTSEENSSKDYLNDLLNFYKEKAKPLEPILDQFGLVLVSASEQKDPWQGRSTVAITLKIMAKAFSAEDDTVVNIIKFLVDDGGLVDREPIVRQEMKEAGVELITLHGSQNSKDLIPIFEEALSSSTDSALKENVIILYGTLARHLQQSDARIHTIIERLLSTLDTPSADIQQAVSACIAPLVFQFKQKVGDYLGILMEKLLNPTVASSMRKGAAWGIAGLVKGYGISALSEFDIIRNLIEAAEDKKEPKRRESVGFCFQYLSESLGKFFEPYVIEILPNILKNLGDAVPEVRDATARATKAIMAHTTGYGVKKLIPVAVSNLDEIAWRTKRGSVQLLGNMAYLDPTQLSASLSTIVPEIVGVLNDSHKEVRKAADESLKRFGEVIRNPEIQKLVPVLLQAIGDPTKYTEEALDSLIQTQFVHYIDGPSLALIIHIIHRGMHDRSANIKRKACKIVGNMAILVDTKDLIPYLQQLIDEVEIAMVDPVPNTRATAARALGALVERLGEEQFPDLIPRLLDTLSDESKSGDRLGSAQALAEVISGLGLTKLDEMLPTILAGVTNFRAYIREGFMPLLLFLPVCFGSQFAPYINQIIQPILSGLADNDENIRDTALKAGKLIVKNYATKAVDLLLPELERGMFDENDRIRLSSVQLTGELLFQVTGISSRNEFSEEDGDHNGEFSGKLVDVLGQDRRDRILAALFVCRNDTSGIVRATTVDIWKALVPNTPRAVKEILPTLTGMIVTHLASSSNVLRNIAAQTLGDLVRRVGGNALSQLLPSLEESLIETSNSDSRQGVCIALYELIESASTETISQFQSTIVNIIRTALIDESATVREAAALSFDVFQDVVGKTAVDEVLPYLLHMLESSDNSDFALLGLQEIMSKKSDVIFPILIPTLLAPPIDAFRASALGSLAEVAGSALYKRLSIIINALVDAIIGTSEDESTKGALELALDRVFLSVNDDEGLHPLLQQIMSLLKSDNIEKRIAVLERLPNFFDKTVLDFDVYIPNFVSHAILSLDDEDQRVVNGNFNALSTLLKKVDKPTLEKLVKPAKQSLALTGRQGQDVAAFKLPRGPNCVLPIFLHGLMYGSNDEREESALAIADVVSKTPAANLKPFVSVITGPLIRVVGERFSSDIKAAILFALNVLFIKIPMFLRPFIPQLQRTFVKSLSDATNETLRLRAAKALGALIEHQPRVDPLVIELVTGAKQATDEGVKTAMLKALLEVIMKAGSKLNENSKTNIVNLVEEEMLGSNDKLAVAYAKLIGSLSEILSNDEAHKILQDKVLNADLDGETGKFAILTLNSFLKDAPTHIFNTGLIDEFVSYILNAIRSPDVYFGENGTIAAGKLLLLEGEKRSPFVKKDAAEPFKIGDENINLLINELSKAVLQPASNSTDVRRLALVVIRTLARFKFDECIKQYFDVVGPSVFSCLRDPVIPIKLAAEKAYLALFKLVEEDDMHTFNEWFAKISDRGNSIETVTGTTIQLRSVGDYTKRVGKRLANVERERIAAGGDAETMFSDRFEDEREIWAVGGVELTTDI