Protein: Mitotic checkpoint serine/threonine-protein kinase bub-1 (EC 2.7.11.1) (Budding uninhibited by benzimidazole 1) (Q21776)
Organism: Caenorhabditis elegans
Function: Serine/threonine-protein kinase essential for spindle-assembly checkpoint signaling. Plays a key role in the recruitment of the checkpoint proteins bub-3, mdf-1 and mdf-2 to unattached kinetochores. mdf-1 recruitment is independent of bub-1 kinase activity. Has a role in the correct kinetochore localization of the spindly-like protein spdl-1. In addition, during meiotic anaphase I, controls the recruitment of hcp-1/2 and klp-19 to the ring-shaped domain formed between chromosomes. Involved in chromosome alignment, chromosome homolog segregation and spindle assembly. In association with bub-3 at the kinetochore region of chromosomes, promotes the onset on anaphase independently from spindle checkpoint signaling and promotes the formation of stable end-on bipolar attachments of chromosomes. Plays a role in nuclear envelope breakdown. Required maternally during embryogenesis and in the zygote for the postembryonic development of several tissues including ventral cord neurons, gonad, intestine and seam cells.
Subcellular location: Cytoplasm, cell cortex {ECO:0000269|PubMed:20729837}. Nucleus {ECO:0000269|PubMed:19526056}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:17339379, ECO:0000269|PubMed:18765790, ECO:0000269|PubMed:18936247, ECO:0000269|PubMed:20729837, ECO:0000269|PubMed:24567362, ECO:0000269|PubMed:25987605}. Note=Localizes to kinetochores during prophase and metaphase. In metaphase, also localizes to a matrix-like structure around the spindle (PubMed:11402065). In anaphase, localizes only to unattached kinetochores which is kln-1-dependent (PubMed:11402065, PubMed:24567362). Also localizes in a ring-shaped domain between kinetochores, during anaphase of meiosis I which is air-2 and kln-1-dependent and between sister chromatids during meiosis II, which is kln-1-independent (PubMed:20729837). Localization to the kinetochore is dependent on bub-3 and on knl-2 (PubMed:17339379, PubMed:25987605). {ECO:0000269|PubMed:11402065, ECO:0000269|PubMed:17339379, ECO:0000269|PubMed:20729837, ECO:0000269|PubMed:24567362, ECO:0000269|PubMed:25987605}.
Interaction partners:
- Mitotic checkpoint protein bub-3
- Mitotic spindle assembly checkpoint protein MAD2A
- Kinesin-like protein klp-19
- Separin homolog sep-1
- Kinetochore protein ndc-80
- WD repeat-containing protein fzy-1
- Aurora/IPL1-related protein kinase 2
- MAD related
- Outer kinetochore KNL1 complex subunit knl-1
- separase
Sequence:
MSHIRVAFAPLDTNPSTCGLETFATQIETLSQMMTAEEMKAELYDCVRRILREQKFDQIRENEAMLRLYKVMGRSSTNLKGRGIYEQLFKKDHFTGSLKFYLQWAEECGKDQMLEEFKDVLKLARDRLSERIEMTAIESGFRDLVDEYFNGESGDMFTRPDETMDLFRFNAGSKKKRRSSVCFLQHNVPINNSGKAAFGPKTKTDLRQACIDRPNYHGISIEEFRFAKWKDTFGEDVDDDYRKRKDSGVVFVKHQVIDTDRQAREEVENRFNANLNPRRRHLSPVSEKTVDDEEEKRSRIYSPLVATKDAHRPALRSKIENPPATVTLSSDTKSASEKDVSDSDDADDDERLKIMTAGRKDGNPPDRSTSISSNYSTASARTSKSGAGLDLMAENKCLEAHAMFSDTVHLASEKTMVLGDDSVFVPERSLATTQIVTDFSVLCDPDPTMTITQERPKKVSNGLNVVYDEAAEPEESQKVEESEVQPEIVLVSPVTQTSPATMFNDIYDDEIEFGFFKPSRGNFVTSTPAQGVHLVNIDEYFGNKEEESTHEQEAPVFVAPTSSTFSKLTRRKSLAANQAVQPSVTESSKPERSDPKDSSIDCLTANLGRRLSIGADEIPNLTENNESEITGCKIRRRSEIIKQGDINPWDETLRKKLMCLVRPPQNMHEFQERAPKIQALRDCEVSGEKLHIQTLIGQGGYAKVYRAVTDDQRTVAVKYEVPSCSWEVYICDQMRNRLKDRGLELMAKCCIMEVMDAYVYSTASLLVNQYHEYGTLLEYANNMKDPNWHITCFLITQMARVVKEVHASKIIHGDIKPDNFMITRKIDDKWGKDALMSNDSFVIKIIDWGRAIDMMPLKNQRFKGRAGTEAFDCPEMVDGRSWTYQADYFGFAATMAVVVAGKYAQLTGASVGDYSLNVDIKRRNILRDACYDVINRFLNIPSCDSLPDWNILIKSFSEIWNEKFEASGWRQAVSKFNEACDLAANQK