Protein: Helicase SEN1 (EC 3.6.4.-) (tRNA-splicing endonuclease positive effector) (Q00416)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: ATP-dependent 5'->3' DNA/RNA helicase required for the expression and maturation of diverse classes of non-protein-coding RNAs like precursor tRNAs, rRNAs and small nuclear (snRNA) and nucleolar (snoRNA) RNAs. Directs RNA polymerase II transcription termination on snoRNAs as well as on several short protein-coding genes. May also play a role in transcription-coupled nucleotide excision repair.
Subcellular location: Nucleus {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:8544822}.
Interaction partners:
- Protein NRD1
- Nuclear polyadenylated RNA-binding protein 3
- DNA-directed RNA polymerase II subunit RPB1
- 5'-3' exoribonuclease 2
- Exosome complex exonuclease RRP6
- Ribonuclease 3
- Protein PCF11
- Transcription elongation factor SPT5
- Ribonuclease H
- Serine/threonine-protein phosphatase PP1-2
Sequence:
MNSNNPDNNNSNNINNNNKDKDIAPNSDVQLATVYTKAKSYIPQIEQVYQGTNPNIQEAKLLGELLQVLAEVPKGTHLFCDPILEPISIFSLTIFSFNEEATATWLKNHFNPILSVCDKCILNFARGKCKMLQHFAIQRHVPHEHVAKFNDIVCQWRVEAVFPILRNISVNDNTGINITNEIETAMYECLCNPHMLRLNKQLKATFEAIFKFFYDTKHRLLDVTNPLSIKTFISGVIFCWCEGSKEENEWSRAFLKDLYSRNFHINLSNLTPDIIEEVYIHILFLQNPANWTEIVVSQFWSRLLPVFNLFDKDVFIEYFQVPKNVESLKKTFKFPLEPIFKMWYNHLSKSYHDKPLDFLLRGLTMFLNKFGSEFWSKIEPFTFHSILDIIFNRDSFPIKLIKIQDNPIVEHQTEVYFQLTGSVTDLLSWTLPFYHALSPSKRIQMVRKVSMAFLRIIANYPSLKSIPKACLMNSATALLRAVLTIKENERAMLYKNDEFETVLLTKTDSRALLNNPLIQDIIIRSASNPNDFYPGLGAASASVATSTMMVLAECIDFDILLLCHRTFKLYSGKPISEIPISTNVLENVTNKIDLRSFHDGPLLAKQLLVSLKNINGLLIVPSNTAVAEAHNALNQKFLLLSTRLMEKFADILPGQLSKILADEDASQGFWSCIFSSDKHLYQAATNILYNTFDVEGRLEGILAILNSNLTVNLKNINVMLQRLINCEFYEPCPRAVRVLMDVVSAFVDPISGVFANFQTLKSQNTEKEFLKFWESCWLFLDTIYKFTLKWASKYDYSELENFTKDTLDLSRSLVDSFREFSDILHDQTKNLLLNVLETFKNMLYWLRLSDEVLLESCVRLIISTSDLAHEKHVKVDDSLVEMMAKYASKAKRFSNKLTEQQASEILQKAKIFNKALTEEVATEAENYRKEKELSRLGKVIDLTDSVPASPSLSPSLSSTIASSSAESRADYLQRKALSSSITGRPRVAQPKITSFGTFQSSANAKLHRTKPVKPLSKMELARMQLLNNRVVHPPSAPAFHTKSRGLSNKNDDSSSEESDNDIESARELFAIAKAKGKGIQTVDINGKVVKRQTAAELAKQELEHMRKRLNVDMNPLYEIILQWDYTRNSEYPDDEPIGNYSDVKDFFNSPADYQKVMKPLLLLESWQGLCSSRDREDYKPFSIIVGNRTAVSDFYDVYASVAKQVIQDCGISESDLIVMAYLPDFRPDKRLSSDDFKKAQHTCLAKVRTLKNTKGGNVDVTLRIHRNHSFSKFLTLRSEIYCVKVMQMTTIEREYSTLEGLEYYDLVGQILQAKPSPPVNVDAAEIETVKKSYKLNTSQAEAIVNSVSKEGFSLIQGPPGTGKTKTILGIIGYFLSTKNASSSNVIKVPLEKNSSNTEQLLKKQKILICAPSNAAVDEICLRLKSGVYDKQGHQFKPQLVRVGRSDVVNVAIKDLTLEELVDKRIGERNYEIRTDPELERKFNNAVTKRRELRGKLDSESGNPESPMSTEDISKLQLKIRELSKIINELGRDRDEMREKNSVNYRNRDLDRRNAQAHILAVSDIICSTLSGSAHDVLATMGIKFDTVIIDEACQCTELSSIIPLRYGGKRCIMVGDPNQLPPTVLSGAASNFKYNQSLFVRMEKNSSPYLLDVQYRMHPSISKFPSSEFYQGRLKDGPGMDILNKRPWHQLEPLAPYKFFDIISGRQEQNAKTMSYTNMEEIRVAIELVDYLFRKFDNKIDFTGKIGIISPYREQMQKMRKEFARYFGGMINKSIDFNTIDGFQGQEKEIILISCVRADDTKSSVGFLKDFRRMNVALTRAKTSIWVLGHQRSLAKSKLWRDLIEDAKDRSCLAYACSGFLDPRNNRAQSILRKFNVPVPSEQEDDYKLPMEYITQGPDEVKSNKDTKKRRVVDEGEEADKAVKKKKKEKKKEKKKSKADDKKKNNKKAESPSTSSGTKKKSSIFGGMSVPSAVVPKTFPDVDSNKKAAAVVGKKKNNKHVCFSDDVSFIPRNDEPEIKVTRSLSSVLKEKQLGLKETRTISPPEISNNEDDDDEDDYTPSISDSSLMKSEANGRNNRVASHNQNFSASIYDDPQVSQAKQTQVPAAITKHRSSNSVLSGGSSRILTASDYGEPNQNGQNGANRTLSQHVGNANQYSTAPVGTGELHETLPAHPQDSYPAEAEDPYDLNPHPQPQSSAFKGPGSGPTGTRNSSRRNASSSPFIPKKRKPRS