Protein: Receptor-type tyrosine-protein phosphatase-like N (R-PTP-N) (105 kDa islet cell antigen) (BEM-3) (Brain-enriched membrane-associated protein tyrosine phosphatase) (ICA105) (ICA512) (PTP IA-2) (PTPLP) [Cleaved into: ICA512-N-terminal fragment (ICA512-NTF); ICA512-transmembrane fragment (ICA512-TMF); ICA512-cleaved cytosolic fragment (ICA512-CCF)] (Q63259)
Organism: Rattus norvegicus (Rat)
Function: Plays a role in vesicle-mediated secretory processes. Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets. Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation. Plays a role in insulin secretion in response to glucose stimuli. Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain. In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Required to maintain normal levels of renin expression and renin release. Seems to lack intrinsic enzyme activity.
Subcellular location: Membrane {ECO:0000305|PubMed:7568143, ECO:0000305|PubMed:7657822}; Single-pass type I membrane protein {ECO:0000305}. Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000269|PubMed:11483505, ECO:0000269|PubMed:8641276}; Single-pass type I membrane protein {ECO:0000305}. Perikaryon {ECO:0000269|PubMed:8641276}. Cell projection, axon {ECO:0000269|PubMed:8641276}. Synapse {ECO:0000269|PubMed:8641276}. Cell membrane {ECO:0000269|PubMed:8641276}; Single-pass type I membrane protein {ECO:0000305}. Endosome {ECO:0000269|PubMed:8641276}. Note=Detected on neuronal secretory vesicles, but not on synaptic vesicles. Colocalizes with insulin-containing secretory granules. Primarily detected on secretory vesicle membranes. Transiently found at the cell membrane, when secretory vesicles fuse with the cell membrane to release their cargo. Is then endocytosed and recycled to secretory vesicles via the Golgi apparatus membranes. {ECO:0000269|PubMed:8641276}.; SUBCELLULAR LOCATION: [ICA512-transmembrane fragment]: Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000269|PubMed:11483505, ECO:0000269|PubMed:8641276}.; SUBCELLULAR LOCATION: [ICA512-cleaved cytosolic fragment]: Nucleus {ECO:0000250|UniProtKB:Q16849}.
Interaction partners:
- Receptor-type tyrosine-protein phosphatase N2
- Glutamate decarboxylase 2
- Proton-coupled zinc antiporter SLC30A8
Sequence:
MRRPRRPGGPAGCGGSEGSGGLRLLVCLLLLSGRPGGCSAISAHGCLFDRRLCSHLEVCIQDGLFGQCQAGVGQARPLLQVTSPVLQRLQGVLRQLMSQGLSWHDDLTQYVISQEMERIPRLRPPEPHPRDRSGSVPRRAGPAGELLSQGNPTGSSPAVQGLSRPPGDGNGAGVGSPLSSLQAELLPPLLEHLLMPPQPPHPSLTYEPALLQPYLFQQFGSRDGSRGSESASGVVGHLAKAEDPVLFSRSLSKAILGTHSGHSFGDLTGPSPAQLFQDSGLLYMAQELPVPGRARAPRLPEEGGSSRAEDSSEGHEEEVLGGHGEKSPPQAVQADVSLQRLAAVLAGYGVELRQLTPEQLSTLLTLLQLLPKGTGRHLGGAVNGGADVKKTIEEQMQRGDTADARPPTPLLPGHPTASSTSIKVRQVLSPGFPEPPKTSSPLGISAVLLEKKSPLGQSQPTVVGQPSARPSAEEYGYIVTDQKPLSLVAGVKLLEILAEHVHMTSGSFINISVVGPAVTFRIRHNEQNLSLADVTQQAGLVKSELEAQTGLQILQTGVGQREESAAVLPRQAHGISPMRSLLLTLVALAGVAGLLVALAVALCMRHHSKQRDKERLAALGPEGAHGDTTFEYQDLCRQHMATKSLFNRAEGQPEPSRVSSVSSQFSDAAQASPSSHSSTPSWCEEPAQANMDISTGHMILAYMEDHLRNRDRLAKEWQALCAYQAEPNTCATAQGEGNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPIIEHDPRMPAYIATQGPLSHTIADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGSSLYHVYEVNLVSEHIWCEDFLVRSFYLKNVQTQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKCYRGRSCPIIVHCSDGAGRTGTYILIDMVLNRMAKGVKEIDIAATLEHVRDQRPGLVRSKDQFEFALTAVAEEVNAILKALPQ