Protein: tRNA nuclease CdiA-2 (tRNase CdiA-2) (EC 3.1.-.-) (CdiA-Bp1026b) (Toxin CdiA-2) (Toxin CdiA-II) (I1WVY3)
Organism: Burkholderia pseudomallei (strain 1026b)
Function: Toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. CDI modules allow bacteria to communicate with and inhibit the growth of closely related neighboring bacteria in a contact-dependent fashion. The C-terminal 301 residues (the CT fragment) cleaves near the C-terminus of E.coli tRNA1B(Ala), probably preventing tRNA charging, and inhibits growth in E.coli. A truncated CT fragment (residues 2948-3122) has tRNA endonuclease activity on several B.thailandensis tRNAs as well as tRNA2(Arg) where it cleaves after A-70 and U-71. Inactive CT domain binds tRNA, probably in a 1:1 complex. Toxic activity is neutralized by coexpression of the cognate immunity protein CdiI in E.coli, but not by non-cognate immunity proteins from other strains of B.pseudomallei. May use lipopolysaccharide as its target cell receptor. Probably gains access to the cytoplasm of target cells (B.thailandensis strain E264) by using integral inner membrane protein BTH_II0599. Protein BTH_I0359 is also implicated in an unknown fashion in CDI in B.thailandensis strain E264.
Subcellular location: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. Secreted {ECO:0000305|PubMed:25174572}. Target cell, target cell cytoplasm {ECO:0000305|PubMed:25174572}. Note=Secreted to the cell surface by CdiB, its two partner secretion pathway (TPS) partner (Probable). Probably translocated into the target cell cytoplasm as intracellular CdiI inhibits tRNase activity. Toxin translocation into the target cell depends on the proton motive force (pmf) of the target cell, but not on tolA or tonB. The pmf is probably required for translocation across the target cell inner membrane from its periplasm. {ECO:0000269|PubMed:25174572, ECO:0000305}.
Interaction partners:
Sequence:
MNKNRYRVVFNRARGALMVVQENGRASHGSGSRDARAGVVPAWLSLSPFALRHVALAVLVAAGVVPIWVNAQVVAGGAHAPSVIQTQNGLQQVNINRPGASGVSMNTYNQFDVPKPGIILNNSPINVQTQLGGIIGGNPNFQAGDAARLIVNQVNSNNPSFIRGKVEIGGAAAQLVIANQAGLVVDGGGFLNTSRATLTTGNPNFGPDGSLTGFNVNQGLISVVGAGLDTANVDQVDLLARAVQINAKAYAKTLNVVAGSNQVDYNTLNATPIAANGPAPTIAIDVSQLGGMYANRVFLVSSENGVGVANAGDIAAQAGDLTLQANGRLVLSGHTNAAGNMSLSASGGIQNSGVTYGKQSVTITTGADLTNSGALTAQQNLTANVGSLNSTGTLGAGINVDSTVGTSGDLNVTSSGQLTATGTNSAAGNATFTGSGVNLSNSATAANGNLALTATAGDVNLAGSTVSAKGAVNAQASGTVVNDRGNLSSGAGMTLGGGSLSNQGGRANSQGPLSVQMAGTVSNQNGMLSSQSTADVRGSAIQNNAGLIQSAGKQTIAGASIDNSAGRLISLNADGLSVTATGALTNAAGANVSGDPGGVIGGKGDVTVQGNTVTNSGSMSADATLHVIGQSVDNGNGALHAGQTTTVDAGNHLSNAGGRVEGQSAVLNGATLDNSQGTVNAATVSLNGTTLLNHGGTVTQTGTGPMTVAITDTLDNSNNGLIQTRSTDLSLTSTTLINDNGGTITHVGPGTLTVGNGSGTVSNKAGAIASNGRTVLQGKTIDNSAGSASGQTGLSVNAADSITNLGGKLTSNANVDVTAGGALVNDGGELGSKTAATTIHSASLSNLNGKIVSPTLTATVAGLLDNSQNGDFEANQLALTAANLKNQGGHISQWQSGPTTLAVSGTLDNSNGGVIQTNSTDLTLAPAVLDNSKGTITHGGTGTLTLTPGNGAGALQNTGGTIGTNGQAIVKAGSLDNGSGVIAAKLGLSATIAGAMNNTQGLMRSNAALSIISNGALSNHQGHIEAGTPGDTSTLSIQAASIDNTDGAVHDFGTGKMTVQGGSQIVNSHAGGVDGMGQMTGQGDVTIGAASISNTQGGQLMGANLLIQGATLDNSGGQVGNVANATGDVNVAMSGAVTNTNGSITSTRDLSVAASTLLGGGAYSAARDAAINLQGDFTTTPQTQFNIGRDLTFTLPGTFANSANLQSVHNLTVNAGNIVNTGAMTAGSLLSTHSGDLTNYGAMVGGSVAIQASGTVSNLGPVALIGASDTSGLLEIVAHDIENRDDTTLGDSMPTTTIFGLGKVALAGGKDANGNYTNAALINNSSAAIQSGASMELHADKVTNTRRVMQTSGNTSQVDPALLQQLGISMSGCAAYYIAACSGQDVHWINLFHDPNYPDYDPAPIIAALKLQPGGVFTVPPNGGQWNSGYQYTTYEGKATANTVTKLSPGAQIASGGDLDASTVKTFQNYWSSVTAAGNIKQPASLDMDGWGATGQQAPGVTVVYSGYYHYNNYDNSEHNWTLPFGDKPFVGGPGGYTQAAPADVRQYSLPDYRSTWGANGTISGNGVSVNNTAANATIPSLGLLPGQAVPGLTIGTVSGNASGTQSGAAAIKGGTPTWVDPVIASATAVNVLSNLTIPQGGLYRPNSAPNPTYLIETNPAFTRMNNFLSSDYYLNQIGVNPLTTEKRLGDGFYEQQLVRNQVTQLTGKAVLGPYTDLQGMYQSLMLAGAEWSKSLNLPLGMSLSAQQVAALTTNVIIMQTETVGGQQVLVPVVYLAKADQQNANGPLITAGNIDLKNTQVFTNSGTVKADTTLALQGKQIDNAFGALQSGGLTSLDTTGNVDLTSANVKAGSLDLNAGNKLILDTATQTTHQVSRDGATSDKTTLGPAANLNVAGDASIKTGGDFQQNAGNLNVGGNLNANIGGNWNLGVQQTGEHKVVQRANGVSDTDLNSATGSTVNVGGKSAIGVGGDLTAQGARLDFGQGGTVAAKGNVTFGAASTTSTINANSSGDQGNRSYAETRHGSDQALTGTTVKGGDTLNVVSGKDINVIGSTIDLKKGDANLLAAGDVNVGAVTERHVYNSRETHSRSGVVSGTKIASSQDATSTVANGSLISADGVSIGSGKDINVQGSTVVGTHDVALNAAHDVNITTSQDTSQSSTTYQEQHSGLMSGGGLSFSVGNSKLAQQNQSSSVTNNASTVGSVDGNLTVNAGNTLHVKGSDLVAGKDVTGTAANIVVDSATDTTRQAQQQQTSKSGLTVGLSGSVGDAINNAISETQAARESAKDSNGRASALHSIAAAGDVAFGGLGAKALLDGAKGPQAPSIGVQVSVGSSHSSMQSSEDQTIQRGSSINAGGNAKLIATGNGTPKDGNITIAGSNVNAANVALIANNQVNLVNTTDTDKTQSSNSSSGSSVGVSIGTNGIGVSASMQRAHGDGNSDAAIQNNTHINASQTATIVSGGDTNVIGANVNANKVVADVGGNLNVASVQDTTVSAAHQSSAGGGFTISQTGGGASFSAQNGHADGNYAGVNEQAGIQAGSGGFDVTVKGNTDLKGAYIASTADASKNSLTTGTLTTSDIENHSHYSANSAGFSAGASVGVSTKAVGPSSVSGSGGVTPMVFQNDSGDQSATTKSAVSAGAINITKPGEQTQDVANLNRDATNLNGTVSKTPDVQKMLSQQADTMNAAQAAGQTVSQGIGLYADGKRKDAIDAAKAAYERGDLVAMQSYIDQAKSWDEGGASRAGLQATGGALIGGLGGGSVLTAIGGAAGAGTSSLLAGQAEKISKSVGDMTGSSLVGNIAANVAATVGGALVGGSAGAAMASNVELYNAGNDPQKTDDRATIAGLQGLLNQAVAAGAKGLSTIANARNAIGNAISGALDSAADQFGTLMKRDAEGKMSQSPAELVSQGVANGINTVLGSKGGEPPLAGPSAVAVDSLTGQAANAALGATDRTPPSNAILSNSNSDNNSTQGSQSGTVTKTPNPEATGSLSGKPTQIPPLSDEVTTRSLIRENQSAVTLANKGYDVVQNPEVLGPKNPDYTINGQVFDNYAPATGNVRNIATTISNKVSSGQASNIVVNLADSSASPAAIEAQINSYPIPGLGKVIVIDKLGNITIIKPKGN