Protein: E3 ubiquitin-protein ligase highwire (EC 2.3.2.33) (Protein pam/highwire/rpm-1) (Q9NB71)
Organism: Drosophila melanogaster (Fruit fly)
Function: Atypical E3 ubiquitin-protein ligase which specifically mediates ubiquitination of threonine and serine residues on target proteins, instead of ubiquitinating lysine residues (By similarity). Shows esterification activity towards both threonine and serine, with a preference for threonine, and acts via two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates (By similarity). Required in the presynaptic motoneuron to down-regulate the levels of wnd and restrain synaptic terminal growth at the neuromuscular junction (NMJ) together with Rae1 and Fsn.
Subcellular location: Synapse {ECO:0000269|PubMed:10839352}. Cell projection, axon {ECO:0000269|PubMed:10839352}.
Interaction partners:
- F-box/SPRY domain-containing protein 1
- Mitogen-activated protein kinase kinase kinase
- Ubiquitin-ribosomal protein eS31 fusion protein
- Ubiquitin-ribosomal protein eL40 fusion protein
- Nicotinamide-nucleotide adenylyltransferase
- Protein Rae1
Sequence:
MVGLLNPLKYGDHFYELYTNSTRRKMQHDRKVLTKRKSRKDKSAAAMAAAAAAAVLEGNAGAGGLVNPLDPPLVKEQLMILPSFPVAHIELDPNASKFAVFSAIRSTVLEAETRYSLFQEGGAPSGQSGSNQKKWSNNVNSACCMMCAHSCMISNLLDCSCHAHMAMHGAVGPGNAGPGGPVGVLVDGRIPAPVPVVVGGPGLPAEKRPRRDSANSDADSDTEEPTEREPVYATVPLIVGAGLRSLFELIADARHVHPLLCTKALKALLDVIQGQQPESFKLEPEELINPLYDLLLDLATMPAALNSATGAEANWSAMACAALLGLCIARGDTGKMLKAIAAMLMTPRQLSAQIVQLPVVLATLQHTVISAALNKPTRPDFHSHGVPHNSLIDEFPVKLPPLSTGAPITSPAMACDGVFVYLLYGGTLLKIGTGFGGSYKGHIYAQNEDFSHERSAWLGYSGGQLYFRRTCRRSAGDQLQMVGLDTLAIKAMSPLSMLHMREGLNYVLFTDDDSLHAICSNRDDTLVVKKLNLYHNSYNIDPPPFELPLQLARKKFRTLGYAAFEDELLNQYQIQRIQSAHNSFEPKLPAPCRDADVDVMGMACGKEFGLVRASNGRVYYYGKSAALGLKCVGRTPTLKLTELVISKAANIVHVAVGHDGIHALLVNDDGTVFFAGTARRGEDGDSSKNRRQPKAVKPKKMTKIDGHVVVHAACNNGTSAFVTKTGKLIMYGKDTAHCDAMGFVSELLEQHVTKVALGKAHCVALNAKGQLFSFGLNNKGQCGRIFNKLQPVKDVPPFASSSTAAACFASLLPLDKRLKLDFSTLCDYDDHNLVQGQCRVCVICRECTGYNVSCVSALNVPLDQRLAGSICPCGHGDAGCAKCGLCAACIALQDSDEAKTELKPPPSDVQQRQQRSKTLIMRRKERKGELETGAAGGGAATPTDLDKDPPRVAPLAPQLLQLTSSSPVVQVACGLHHTVVLTLAGEVYTFGSNQYGQLGSGDLQPVSGPVRVQVPGAISQVAAGSNHTVLLTSKGMVYTFGNYQKGQLGRLPSDYGLKPPPQDDDSPVGAGSDGGAGGSPTVAPVGMPGPERSQSPANVQPSGSKEMPPLLPVLTQRQKFLWNCSPGAVFGLGPCYGKKVTWIGANGDQTFIKIDESLITAQMLPKMHVVANKKTILLIPSIPLSFHTLSINRRDGSCTAHYRGQTNFVKLMQAQPEQQQPEINSNLDVAVTPVADSPAHASTSSLLAALTGTATAGVPINEQMSRSMHEARNQIFEEQLPEVGSSAAAAAVAAPGTPVSAGSVPRSRRGGKQGTSSPEPIPSPPQLAFTMDPTYNVLWVFDGAARKLRCHNVVASDINDSDANAATYRSLLSPELSLPDRVDSRVARSQASLNLLACLDILTSAQDNIPGCFEQPLLKQTQQTAETQAGEFQVVNRFDNFGGGWGYSGHSVEAIRFSADTDIVICGFGMFGGRGEYSCKLKLFDLGGDGGGYEKEGILISETKEVPYECGARSKHHILLPKPVSAVAGRWYLVWARIAGPSSDCGSCGQASVTTEDQVVFSFKSSKKANNGTDVNSGQIPAILYRLVTQDCKQTPAQMDADPVQRISRAFANSVSRECFESLVVLLSWSWDCFKLQLREERDRSRPLQLQQSLQYLGYVIKSCLRLLRKYTIEIYPQRNSSTSVATGGGSNAAHGSGVVTTAKSVQSKPNKDKNTPRVVGNAGVMAKYFGDPSTSVAPAMISSASSGGAPSTSASAAVAPGSGTPVTRKTNMENIQLAECIGNVRALLIGIFCDDIFKDIATDEGYELSLEILDECHLSFVACFDAFYPTSSLKWNCLCDLLAQMDRGALHSRLLSAILAGLCSPSVKLRATFSLLSAAGNERQSIISPSDNSGLPMLSSTDAHPYPVLVEQMIYRTQQEKSDFLSNSWTFKDVLVRLLDIIASPIRSRIEAIYSRSLGSLGYPGGKDCVNQGLIDNCCHLLARVLAEIVYQTAMGEYDKLFMPPRTLHSTGARFARCDVSRTWNTGNFGPDAIAFAVDRPGVAIAGAMVYSGSGSYDYQLELLYDNTADLQPQHKWETLESVSGSYDQDAVHNDLAEIKFDHPVHIKENARYALRLCSQGARTCSGDAGMPAVRGPCGAQFHFYACDLSFNGTTPARGQLPCILYYSTPMKQDGHSASGRTGDGSNVATHLEDRIMLLGPHEVSTRDTALQIAADITKKCTELLILARNAMAASCSPSDNSSNHTQTIDSEHNITPIEEHMDINWANNSRTAALPTAIDPQLSTARDLGKRIESFSKGLMETLKFDKRSTNPFEMEIEIGATEVEESADLRNGQSQSVSQSQSQSQSVPINGNERTADFEFAEQSAQQSMPQHLHSDSEEAPLEVAGMAAGGGVSVADGSGGVAGVGSQAAAVQLLEVFNLAASNMFHTLLPLVYAHIANLACSDPKSSVQILGLIKEILPHIAALNQLHVSKDQRQPEPAIFATQTSGSGNSNSSSTTSNHYCVVESDHPYKSASISSYRVEFPPCVQWLTIEFDPQCGTAQLEDYLLLSIPMRPASQAPPVPHVDDYLEQADNNVNGAGDRRRNTGGGIAGSGAAPNTHQRSASVQLTMASCCRSPGCGNAPGSAAAPSSMPLRSQDPNDREWIVVKKFNTASTWLHNVLILPGNCVEFSLETASLYAQDPHNNRYGFKCLVVGYDNPTSINASNSCLIRLEQELAYLGGMCSANLMKKELNLPDDKDVEDMSGIEETINTHHTLLSKGFALSEPQLTVHQALESYLPIGSQSNERQFLKDFISGAPGSSGARLAAWLQPESRLDPNKCELNTITEPLRYGWPSQVTVTIRDQYGDAVLVPELKVEIKAIPTGSGPNGSATGTGTSCTSVAEVSAPGPNLWMRRASRDTWGWGGMAPPPRINYEPTVKDKMVFKAITFMKPYANYSFEELRYASPVQTRVTELLNAKDMEDGTFSVQWTPSSVGAYCLAVTIDGIPLEEVYRVDVKEGILPPPTQRNSAQRRPQAPSKLRRFQARHSSGLRIRSHPTLQSEQVGVVRVGGVISFIDEIENDDGVWLRLSTESIRQHCTMGWYPTEAWCLQFNQHLARMLLQPVTDKEVNPVRKGVGAEEDVEEQPPVTPSASGEASPEPEPDPSPVLSPAKTKPGRFLSGHQSTNPFLYPAKHADLAEREAQVQEEREKEEEQVDDEDADDREPEQEALPAVELLPAHIGSAIAGVVGGGAIKLQALQKWFKGDAVDGPQPLTPSHSPPLAGVSVRELVRAMGGQDSPRGNGNRSQQEQDPEFSLASMRRPNYSASQTAALLSTPKHTPKRSAVVASETSGLEDELSLLQITTTTTGQGEQQSELQLATTSTASSASKRNPMGPIKRAMPPSFAESIRAVFAALLWHEGVVHDAMACASFLKFHPGLPKEGATVVTRRGESGDPRLQLSREQKAQQRHSVEVANAGNYLNIRPSTLETLTKSGNCSLHNRSKYRKNLLSGGGGAINSGDDTAQKLQALPEMVSVLPPALRCLVYLWEQICSGCVQIVQSNALEQREPRLLSPGSRDLNGDADTEGKEGKNSDQASAGEKDLGRKCKRKKKDDGSWCEICELFLPMPVTYHMRIAHPGCGKSAKGKGYNSVGIFCEGWAGNCGEGGKGASSWFLMCDPCRDRYLASCRSANNINSAARQLESSAAEGNELNLFGVKSTTLIANAEVYTTMRENATFLLELCSSSSSASGAAGSLAATSSSSKRSPQQMSVVAMPVVIEHQLGNSDLKPSTSRCSRMARLSGSKFCPGVGSGAFRKSFVGGPPTAPENVWLAPESFACLECLGTAGHEDLPYEMFGLGPNSNDNGYDRPLSEISYESCEPNNYDMLSGSLAPGTTAAASVGGGNLSKFHRSYSMGQGWASLAQHNHPPPHHPQQQHHQQQQMNLQLQQHQAPPVDGQPKVVYRRRNNSTSEGDGSLLICYPSEHLRRLVPQKLLASVSVMQTASGEGTGKDHATGTLGLDQSAGQNGGGNLLLTRPAMAFITQKHELDRLRAAMRRSLRIAACRIYALQALNWLLRSVTQGVCLHDLMWWFVSSLNPTGGHQPVERGEEASEPALEHPVAYTQISGRFAHLITQSLHVFLQSVADLTLHLPLGSPLQRVAIQCFGIRFRQADHQFLHSSHVFGNISKILSKSDEQNDAMAVSTILKPDCDVEHNQVHSVATGGSSGAGARLLCYTDLAGMFEVTVSSRPAMAESLTDNSTETFWESDEEDRNKCKIIELSLTKLNYACRYLLVHIDNSRDIQNKVLNVVFYAGQSLGDTNIIKSADVDPKACSWISAKICDDSCTHFRLELHGPENTLRVRQIKLLGLPIGGAVGSDDSSDHKHQPHLRLSHASRIQQQICEAETLRVFRLITGQVFGKLISNVSSDLVPPDSAGIGPPSGGAASTSLLADSLDLREHMVGILFSRSKLSHLQKQVIVHIVHAIRKEAQRAKEDWELANLAHVLKQSPQQQTAPALAASASCESTPERSRAPDTYCFEMLSMVLALSGSVVGRSYLSQQHGLLRDLLGLLHTGSDRVQRQVTALLRRILPEITPESFAELLGVQRLPPADYSIAHQSASDFDMSRLGLLDIFLAVIAKSLQLQVKVKTTVASTGPSGSGGVSGSSSGNGGAVLKAGQQEKTPAFVRLWSSLDLSVQQLRSRPPTGEPGTTDPFQFDALPPRKESKRNLNQRWFLNGVISTKQAESIISLIRDLASGKLSEKWSQITKAAIAESVLNLTRLEEIYRSPEHCTKTSTLWLALASLCVLERDHVEKLSSGQWSKLCDTRPLCSNHDDGETAAIIQCETCGSLCGDCDRFLHLNRKTRSHKRTVCKEEEEAIRVELHESCGRTKLFWLLALADSKTLKAMVEFRDGSHTIISGPQEAVGRCRFCGLTGNSGLLEIGNVCADAQCQEYAANSCLKTKPCGHACGGVTGERKCLPCLQHVCHTRENELAEELRDPKLTQDADDMCMICFVEALSCAPSIHLECGHVFHYHCCKAVLEKRWSGPRITFGFSLCPICKADIQHPLLSDILEPINGLKQDVKRKALMRIKYEGVVKDTDSKNVNMTQLAMDRYAYYVCFKCQKAYYGGEARCDAEIGEKFDPEELVCGGCSDVARAQMCPKHGTDFLEYKCRYCCSVAVFFCFGTTHFCDTCHDDFQRLTNIPKVKLPQCPAGPKAKQLLGDECPLHVMHPPTGEEFALGCGVCRNAQTF