Protein: Protein adenylyltransferase and cysteine protease IbpA (HMW IgBP) (p120) [Cleaved into: Protein p76 IgBP (76 kDa antigen)] [Includes: Protein adenylyltransferase IbpA (EC 2.7.7.108) (AMPylator IbpA); Cysteine protease IbpA (EC 3.4.22.-)] (Q06277)
Organism: Histophilus somni (strain 2336) (Haemophilus somnus)
Function: Adenylyltransferase involved in virulence by mediating the addition of adenosine 5'-monophosphate (AMP) to specific tyrosine residue of host Rho GTPases RhoA, Rac and Cdc42. The resulting AMPylation inactivates Rho GTPases, thereby inhibiting actin assembly in infected cells. Probably also acts as a cysteine protease, which may play a central role after invasion of host cell and in virulence. Possible member (with IbpB) of a 2 partner secretion. Probably able to bind bovine epithelial cells (host cells). May participate in the formation of fibrils at the surface of the bacteria.
Subcellular location: Secreted {ECO:0000269|PubMed:16169703, ECO:0000269|PubMed:9317034}. Note=High molecular weight-immunoglobulin binding protein IbpA, and probably other shorter forms.; SUBCELLULAR LOCATION: [Protein p76 IgBP]: Cell outer membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}; Extracellular side {ECO:0000305}.
Interaction partners:
- Outer membrane transporter protein IbpB
- PFAM: Hemolysin activator HlyB domain protein
- KEGG: msu:MS1167 FhaB protein.
- KEGG: msu:MS1163 FhaB protein.
Sequence:
MNKNCYKLIFSKTRGCLVPVAECITSAVDSGSSDSVVVSEKTDEEDRQGSIEDYRLSNVCLSVKTFLNPVSSALCLNWKSVSVLLLSMVAAPNFAQSAEEAAKAEKTPKLTEIQNGNDGIQLETKNQNIGVGAGTTENNHPTKLYKTENNVIVIDIAKPNDKGISDNRFQKFNIPNGAVFKNNKDQQRSELVGYLEGNKNLADKEAKVILNQVTGSELSQIKGALEILGTKADLVIANQHGINLNGVQTINAGRFVATTSKLIDPNKMEFDVTQGTVTIDVNGFATDNLPYLDIVAKKIEQKGTIGNKEKEKNKTSETEITFIAGKGKIKYNIENDGKTKLEVQKDSNTSQPSDKEEVAITGASTGAMHGKSIKLIVTEQGAGVKHDGIILSENDIKIESNKGDIDLGDKLQAKNEISLNNAKRITIANEITADKSITITADDVKLKNNKEASATEEAKLKGKGKLASKKVKVEAKKSLVLDDETKVVATDLELKSQTLTNQGRIYGNKVKIDTDKLVNKKEIYAEDNLDITTKGKTVTVSVNKDNKRKADVKEETVADLDVGFENTGTIESKSKAKLTFKDNTSFVSKGNKFIKAKDELTIDAQNVVISENDELQTTARLTINAAGNVVNNGLLASGKTLTINAKQGSIYNEKGILGAREQLTLSAKGNNKETEGNIINGADSLLHSEGKMELDAENTVYNLGNIFAKSDLTVKANELINDVKLSGSITKKSPYSVLNRYRRSDIASHGWHNNDYRLWINPIEFEKAEVKVEKAGLIRAEGNFKFEGKKGDNQQDATLTNHGVINVKNTFEAQNAKVVNNMKAYQANLLTEFFKQKQDITFNYQPRARLFLSALSGQAERKFNSLEELFDGLFSEQPITNSSSYYADNSQAVHLLEEIKSPTFQKAMTLVFGANWKNEDHKKLSQRWKEFKEKQDAHFDYRPTDKAKILAQRINGKIDELKNGSTGGFSESERITVGQHKFDLSKVEFRSEVNRKENLNNSNVDLSALSDLLSIPNLFVDNSVQLDKTVDKNIEIDEEDEFLLKPHTGEEPDLLNENELSENGKFLDKLLGEIGEKTYIREVSDDWERDPDEPDEPDYKTESRLETRDRFDTLPSEVQDKLRQKFNEYKEKAQQKRQAEALQAKTKNEQLQSDLETGYKEEEKRQAKNDLEKQAELQQLDQQEKEKLAKEKELQGKINEEKQQEALAKQKQEQQKQADAKAKIEEEKRLEEYRKELAKDHQIEEALSKNQFLKEVDDTRPKVETDPLYRTKLQYINQDEYFGSKYFLNKVGSSTDAGKKVAVIGDNYLEHQLITKSIEKKVDNHLALKYQVNDAQLVKKLIDNSYFESKELGLKVGEALTKEQQNQLKQDIVWYVKANINNKEVLLPQVYFANKTLRDAEKFKGLGDALIRANEINLKTRDVLNSGTISGKNIDIEAENKIKNRGDILSEESTRLVGHKGIDNTARSFVNGNGDVEVQRASIRTEGHLHLEADEDSDINSKGSDIKGKTGFVKARNFNTTDTHRTEHSVEKGRIFSKKGEILGYRKESTQKAISVGSNTEFDHVHFAIKNDVNQEGSKIKAKVVTGVVQGDYNTKAGRNAQQTERYIRLDQEYSSGHISGAGFTVSHERDSQNGEKTNIGGASSNTGTGFTLGGSFSETREKETSLTHTNSDLQVDHGILHVLKKAEIGGVDINKHKFTGKAVEEDEAKAEQQAKAKAAPDATDNAAQKEEPKFKVLSQSEVDDLMTEKSANDLFNKYKKVKEDEGFELSAKEITSNKQKDEYHLDSERSVLKFGIETEGHSAIADAVSHVAKEIVEAQRGVKQDGTVALQHISDVANIVTGELVGGSSKFGFERNYETNKVKETSDIRTKIAGNITLSAHGGNLQLKNVESDANSKLTLQAKRNVDILDGETTRESTERQSRQKFAFGINSGCSVMSGGCNGGVSGSVDGNESFTTEKSVTHNNSLLRAKNLKIAAGKDLNLISSNIKADHLDLNIKGKTNIVSKQDSFDRLYRGFDFSASAGAALSSSTLVKGNGSFGAGYTHEVENRKLLNQQAGIVANRITGQIKDLDLVAAHFINKDENSGFRVSGNVTSQQLNDSHHKDGGSVGVSVGINERGASSFNVRGGRAEQKHYDAVQKSVISGINLKDNNVTGEIVDDLSKAKTVTRDDVYASTQFNFEVADLVELGEKAKSKLQSKFSKAVNNDAEQPTTTRISSEDVVEMVDNPLYGSNADVRKLRTLDEVGEGYSTLGDQNANKGRKLPNGSDDIYSLLGKVKVSGDEPVYDKVSAEGAYDLLGDSNANKGRTLRNNSDDLYSTVGDANSDISRIRSNVYDEIAAGPYSLLGRTKAAEEHIYEQIGEGPYSLLGNGSAVRNRTLGGESNSTYSTVGDANSDISRIRSNVYDEIVAGPYSLLGKPKAAEEHIYEQIGEGPYSLLGNGSAVRNRTLGGESDSPYSTVGDANSDISRIRSNVYDEIVAGPYSLLGRTKAAEEHIYEQIGEGPYSLLGNGSAVRNRTLGGESDSPYSLLGGEGTRNKVLADTIESIYSTLSRPQASSNLEMVDNPLYDSVRRSASDQLPELPTVRNLLNSDTEAGNGTYSEITSRTRNANDPLPPLPNEFRTRLSQGADLADHVYDTIGSIYSVLSKPKASSNLEMVDNPLYGSVRRAAGDQLPELPTVKTLLNKVEEVGNEIYSEITSKTRSANDPLPALPNFRLTQEVDTADHIYADINDVVNRANKAKRDLPATPEATPKVAVDGGDYATIGEVSPLQPRASRQQGSSDYEEIPLPQETAPQKTSPVKRTSAEGEDGYATIAEVLQPRAAKGQVSDYETIPLDEPSQAAVRTERSAVEGDYAEITSPSIQPRSARGQSGGEEFEPFPSEFSSEPQSPKRALPAENAVVNELGNELKARLKSKEDQANPAKAEVSEPIYATLDKSPEGLARAKAKGDEAAAANPIVKTRVEDDVAPELPARPSNLSDSISNETIAENGQSVALGTPKSAVAESNRNNNGNQKLQSEGAEGVSPKTKSEDKSWFAKVKDFFFAKSNKSQAKEAKSEQETVSKPNYDSLEDDLNLKNLLALEDKRGSSFEENVLKNPEFLAEAREIAKKYIPEATIKQMGNSPEFDEILTEGAKKVEKRINDALTFKPSVDEFNEIQGLVKNIQKGSAVDDLNAQTLAITEALADTSKTIQRNPKLKEEVQGAIEEFLKSSQGKELTVEMIEKLNHGLRPDEGSDRLLYKKENLTKENAVFSSPQASKIQLNETVDFINQAIKQNVEPSVLAGLVYQRLIAYHPFAEGNGRMARVVVNKILLDAGYPPFTKFSSEFETQIIPQTKATAKSATSAEVVKEFLTELGKKSSPQEGGANNQNGQATSPVTLKSKDVSEVENTQSADSLTIKQPEQGKAGGQLPSVPKVETSVNEVAPLSSVPAELKDAAGGNKKAAEKSEGATGVEKEKTTLFQRVKQFFTGSKSGAKPVAGDETANKVNYQDLEDNLNLKGLISLEDDRNANFESNVLKNEKFLDEAREISKKSIPEATVKQMSHLPEFDDILTEGAKKVESRINKAITFRPSVEEFSEIQDLVKTLPKTKVIEDLSTKTNEITEALAATSKTIQRTPELKEQLKTAIEDFLQNSQGKPLTVQMIENLNHGLRPDEGEGRLLYKKENLTKENAVFSSPEAAKIQLAETVDFINRAKNEGIEPSVVGALVYQRLIAYHPFAEGNGRMARVIVNKILLDAGYPAFTKFSDEFEPQIIPQTKASTKSATSSEVVVEFLKELAKKGSKEDNEQNLEKTDRTSTDLTESAVENSAALSSGTVRSATVSETVTETEQAKAKPVSDLVSSKDLVEQQRTVLQRIQDQFQPLKVKSKIDAVRSSVEEFGGEVSFKFAQSKGEVYKEIVKHIETQNGVCESTCAHWIAKNVNPTDENFFNTLYEGGKKGHLKKETIDSIKKLQTEFINSGSATQQFKLTDSWLQEQGVVPKEKKVADFVRRDEVSGTVSKNDVSSLVKAILDTGDDTAGVKKISINLEGGSHTVSAAVDGSKVTFFDPNFGEMTFPTHQQFENWLKNAFWQKSGYAGKQEGRRFFNVVNYKKNN