Protein: DNA topoisomerase 2 top-2 (EC 5.6.2.2) (DNA topoisomerase II) (Q23670)
Organism: Caenorhabditis elegans
Function: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks (By similarity). Essential during mitosis in the adult germline and during embryogenesis for proper segregation of daughter chromosomes. Required for centromere resolution during mitosis. Required for chromosome segregation in anaphase of meiosis I during spermatogenesis. Promotes cleavage furrow stability during cytokinesis upon the presence of chromatin obstructions. Promotes DNA break formation upon zygotic genome activation in the Z2 and Z3 primordial germ cells in L1 larvae, thereby activating a checkpoint response. Essential for embryogenesis.
Subcellular location: Nucleus, nucleoplasm {ECO:0000269|PubMed:27707787}. Chromosome {ECO:0000269|PubMed:27707787}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:27707787}. Note=In the hermaphrodite and male germline, localizes to the nucleoplasm in the proliferative mitotic zone except in metaphase where it localizes to the spindle. In the transition zone, associates with chromosomes and localizes along the length of chromosomes in pachytene nuclei. In diplotene, starts to relocalize to the nucleoplasm. In the oocytes, it is completely disassociated from chromosomes in diakinetic nuclei. In the male germ line, it is mostly nucleoplasmic in karyosome nuclei. In male meiotic metaphase, surrounds chromosomes and a small pool is detected in the cytoplasm. In meiotic anaphase, encircles the chromosomes and localizes between the segregating chromosomes. May co-localize with nmad-1 on DNA (Probable). {ECO:0000269|PubMed:27707787, ECO:0000305|PubMed:31283754}.
Interaction partners:
- DNA topoisomerase 1
- Small ubiquitin-related modifier
- 5'-tyrosyl-DNA phosphodiesterase
- SUMO-conjugating enzyme UBC9
- DNA topoisomerase 3
- Germ cell nuclear acidic-1 protein
- Mitotic chromosome and X-chromosome-associated protein mix-1
- DNA replication licensing factor MCM7
- Meiotic recombination protein spo-11
- Structural maintenance of chromosomes protein 5
Sequence:
MSDSDSEFSIEDSPKKKTAPKKEKASPKKKKDDANESMVMTEEDRNVFTSIDKKGGGSKQMAIEDIYQKKSQLEHILLRPDTYIGSVEHTEKTPMWVYNMEESKLEQRDISYVPGLYKIYDEILVNAADNKQRDPKMNTIKITINKEKNEISVYNNGKGIPVTQHKVEKVYVPELIFGTLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTKFTLETSSRDYKSAFKQTWIKNMTRDEEPKIVKSTDEDFTKITFSPDLAKFKMKELDDDICHLMARRAYDVAGSSKGVAVFLNGKRIPIKGFEDYVQMYTSQFNNEGEPLKIAYEQVGDRWQVALALSEKGFQQVSFVNSIATTKGGRHVDYVADQMVAKFIDSIKRKLTKTSMNIKPFQIKNHMWVFVNCLIENPTFDSQTKETMTLQQKQFGSTCVLSEKFSKAASSVGITDAVMSWVRFKQMDDLNKKCSKTKTSKLKGIPKLEDANDAGTKNSQQCTLILTEGDSAKTLAVSGLSVVGRDKYGVFPLRGKLLNVREGNMKQIADNAEVNAMIKILGLQYKKKYETEDDFKTLRYGKLMVMADQDQDGSHIKGLVINFIHHFWPSLIQRNFVEEFITPIVKATKGKEEVSFYSLPEYSEWRMNTDNWKSYKIKYYKGLGTSTSKEAKEYFLDMVRHRIRFKYNGADDDNAVDMAFSKKKIEERKDWLSKWMREKKDRKQQGLAEEYLYNKDTRFVTFKDFVNRELVLFSNLDNERSIPCLVDGFKPGQRKVLFACFKRADKREVKVAQLAGAVAEISAYHHGEQSLMGTIVNLAQDYVGSNNINLLLPIGQFGTRLQGGKDSASARYIFTQLSPVTRTLFPAHDDNVLRFLYEENQRIEPEWYCPIIPMVLVNGAQGIGTGWSTNIPNYNPRELVKNIKRLIAGEPQKALAPWYKNFRGKIIQIDPSRFACYGEVAVLDDNTIEITELPIKQWTQDYKEKVLEGLMESSDKKSPVIVDYKEYHTDTTVKFVVKLSPGKLRELERGQDLHQVFKLQAVINTTCMVLFDAAGCLRTYTSPEAITQEFYDSRQEKYVQRKEYLLGVLQAQSKRLTNQARFILAKINNEIVLENKKKAAIVDVLIKMKFDADPVKKWKEEQKLKELRESGEIELDEDDLAAVAVEEDEAISSAAKAVETKLSDYDYLVGMALIKLSEEEKNKLIKESEEKMAEVRVIEKKTWQDLWHEDLDNFVSELDKQEAREKADQDASIKNAAKKLAADAKTGRGPKKNVCTEVLPSKDGQRIEPMLDAATKAKYEKMSQPKKERVKKEPKEPKEPKKVKKEGQDIKKFMSPAAPKTAKKEKSDGFNSDMSEESDVEFDEGIDFDSDDDGVEREDVVSKPKPRTGKGAAKAEVIDLSDDDEVPAKKPAPAKKAAPKKKKSEFSDLSGGDSDEEAEKKPSTSKKPSPKKAAPKTAEPKSKAVTDFFGASKKNGKKAAGSDDEDDESFVVAPREKSGRARKAPPTYDVDSGSDSDQPKKKRGRVVDSDSD