Protein: YTH domain-containing protein 1 (RA301-binding protein) (Splicing factor YT521) (Q9QY02)
Organism: Rattus norvegicus (Rat)
Function: Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (By similarity). Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (By similarity). In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (By similarity). May also regulate alternative splice site selection. Also involved in nuclear export of m6A-containing mRNAs via interaction with SRSF3: interaction with SRSF3 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (By similarity). Involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts, probably by binding m6A-containing MAT2A mRNAs (By similarity). Also recognizes and binds m6A on other RNA molecules (By similarity). Involved in random X inactivation mediated by Xist RNA: recognizes and binds m6A-containing Xist and promotes transcription repression activity of Xist (By similarity). Also recognizes and binds m6A-containing single-stranded DNA (By similarity). Involved in germline development: required for spermatogonial development in males and oocyte growth and maturation in females, probably via its role in alternative splicing (By similarity).
Subcellular location: Nucleus {ECO:0000269|PubMed:10973987}. Nucleus speckle {ECO:0000250|UniProtKB:Q96MU7}. Note=Localizes to a novel subnuclear structure, the YT bodies. {ECO:0000269|PubMed:10973987}.
Interaction partners:
- Serine/arginine-rich splicing factor 3
- Similar to KIAA1627 protein
- Serine/arginine-rich splicing factor 10
- Pre-mRNA-splicing regulator WTAP
- Zinc finger CCCH type containing 13
- RNA demethylase ALKBH5
- Heterogeneous nuclear ribonucleoproteins C1/C2-like
- Cleavage and polyadenylation specificity factor subunit 5
- Heterogeneous nuclear ribonucleoproteins A2/B1
- Cleavage and polyadenylation specificity factor subunit 2
Sequence:
MAADSREEKDGELNVLDDILTEVPEQDDELYNPESEQDKNEKKGSKRKSERMESIDTKRQKPSIHSRQLISKPLSSSVSNNKRIVSTKGKSVTEYKNEEYQRSERNKRLDADRKIRLSSSSSREPYKSQPEKPCLRKRDSERRAKSPTPDGSERIGLEVDRRASRSSQSSKEEGNSEEYGSDHETGSSASSEQGNNTENEEEGGEEDVEEDEEVDEDGDDDEEVDEDAEEEEDEEEDEEEEDEEEEEEEEEEYEQDERDQKEEGNDYDTRSEASDSGSESVSFTDGSVRSGSGTDGSDEKKKERKRARGISPIVFDRSGSSASESYAGSEKKHEKLSSSVRAVRKDQTSKLKYVLQDARFFLIKSNNHENVSLAKAKGVWSTLPVNEKKLNLAFRSARSVILIFSVRESGKFQGFARLSSESHHGGSPIHWVLPAGMSAKMLGGVFKIDWICRRELPFTKSAHLTNPWNEHKPVKIGRDGQEIELECGTQLCLLFPPDESIDLYQLIHKMRHKRRMHSQPRSRGRPSRREPVRDVGRRRPEDYDIHNSRKKPRIDYPPEFHQRPGYLKDPRYQEVDSFTNLIPNRRFSGVRRDVFLNGSYNDYVREFHNMGPPPPWQGMPPYPGIEQPPHHPYYQHHAPPPQAHPPYSGHHPVPHEARYRDKRVHDYDMRVDDFLRRTQAVVSGRRSRPRERDRERERDRPRDNRRDRERDRGRDRERERERICDRDRDRGERGRYRR