Protein: Phosphatidylinositol 3-kinase piki-1 (EC 2.7.1.137) (Phosphoinositide-3-kinase class 2) (PI3-kinase class 2) (PI3K class 2) (G5EDY0)
Organism: Caenorhabditis elegans
Function: Phosphatidylinositol 3-kinase involved in clearance of apoptotic cell corpses by phagosomes. Phagosome maturation requires two sequential and non-overlapping pulses of phosphatidylinositol-3-phosphate (PI3P) on the vesicle surface which mediates recruitment of sortins snx-1 and lst-4 and small GTPases rab-5, rab-2 and rab-7. The first pulse is initiated by piki-1, then maintained by vps-34 which also produces the second pulse. Unlike vps-34, not involved in the formation of PI3P in early endosomes.
Subcellular location: Cell projection, phagocytic cup {ECO:0000269|PubMed:22272187}. Cytoplasmic vesicle, phagosome membrane {ECO:0000269|PubMed:22272187}; Peripheral membrane protein {ECO:0000269|PubMed:22272187}. Cytoplasm {ECO:0000269|PubMed:22272187}. Note=Localizes transiently to pseudopods and nascent phagosomes during cell corpse engulfment. {ECO:0000269|PubMed:22272187}.
Interaction partners:
- Phosphatidylinositol-3,5-bisphosphate 3-phosphatase mtm-1
- Rho-GAP domain-containing protein
- Phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR6
- 1-phosphatidylinositol-3-phosphate 5-kinase
- Phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR3
- PIPK domain-containing protein
- Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase daf-18
- Phosphoinositide 3-kinase adapter subunit
- 1-phosphatidylinositol 4-kinase
- Phosphatidylinositol 4-kinase type 2
Sequence:
MSDDEELQLAIEISKKTFKDEQKLRSNDLDLIRFESPDEPARQRKINQIKQLYEANSPGPSSYSGSLATSPIDFRPVYNEPRAGPIPHSQSYPRNYFQDWSAIASTSQPPAFPPPPRPPKPEQYKFPPAPSVPLLHDRYFVPPPPPVPPRHSRVQQSPPVPIHPTPPVSSTPLRHSAPSFASDSQQFLSPIKPFEISFNSTVDTSSNQTGSHDHSIQYQPLTHLYVPYVMHSLNSSYGALLNGDLIDLSAFEDSSNASQDEIRKEFDPLFISTYSTDTPSPDNSMPAVNAYFSKPIDEPECVGGAKLIQENIEFPSSSFCLIDCPNGIEEQVKKLCKRNLIRKDMTPDFFIAPTVDYMVTTASTVKVVVYKDHSWKANKSNGKAMICAIDEKMDIITTQALSLFDSELPTDKEYGLKIYGLNQFLSSDSLLGSNLYTGHCLLNGDDVKLDLGVFAPNSRIYEQTLESWNLMKSQVKYSTVVDKEDVENTLGHLASEMSQYEIAFNDGSTLKLSSSSQRVKQVIMLLCKCLHGIVPEKLYNEMQKYLASTTEDQLVHHRNDFLREIHSFLELYCRCTVSRYNIPPLQIITKPKVEVLSKMDFLQIMLNSVHSIPEHWQSQYSEFYMSLDLYHGTQVLDGFSNKVPKTIKNDHFFPRIPLDLYAKFKRLNLCQYPRETRIVVSISGTVRNSAQAANEYNPDIVMLGYCSVPLYDENLFMRQGPLFLPLTLLKKQPMLKPFGPYPYIKDARDPILIMSFKIWDTEIYFPNVVIDMQCIPQDFATLDIETQEYLLELIENQDTSTLETDDQDLIWQKRLHLTNQPEALPLVLSSLQDWSFGFVMRVYQILEEWAPLRPEIAMEFLLPKYPDERIRAHAVQSLARGSTDFLYHTIPQFIEALRFELYEKSALADFILELSFVSLDFTFEIYWQLQQRVDHCAVDDLPYAIRCQNLQQKMIDEHENPNLKTDIKLQHELLNELDSIQDDLRSKSGDSEIERLHRLRTRLGILDSKLLQNKVRLPICPAFDCTGVRIEECSVFNSNAKPLKIVFRGLNMNYSIIHKRDDDMRQDAFVMKMLNEMDRIWKSNGLDLRMITFRIMPVGYRRGMGELVLNCATLMEIQKEEGLRGVLNDEILRKWLMKHNSDEFAYKEAQENFIRSCAGWCIVTYVLGIGDRHNDNILFTKNGHVFHIDFGKYMGDWQMAAGFRRDRVPFVFTTEMFHVINNGRAPTQYNQKFIDYCCKAFNHLRRNKNTLTNLLRIMACSDIPGINMDSLAFVENNLMLDLSDTDATVQFTAMIQNSLGSAFVRLNFVAHTVAQFISSRPSFSKQDPNKLSFVPELYTENSDGRISRVTVLKFEKHCIPNKIYMYKVEVHRKNVAVSSFIYRSFAEFEELHTKLRARFPMMAVSLNTISNLRSNVRAVAQKRIIHVQKFLIYLFNQVDEICHCDLVYTFFHSILRDNKCDTYIDESLDMPSQCQIYLKIEYNSVKETLSVFIGHAKYLALLQNNQQPDPYVKTYVRPDLRNQSKQKTQVVRGTRHPTFNQDLNYTEFPIEILSTRVLEVSIWNNGGYLVKHKMYMLCIPLLKVKKLAESRKNCRTLEGWFNCEKCV