Protein: Reticulocyte-binding protein homolog 1 (PfRH1) (Normocyte-binding protein 1) (PfNBP1) [Cleaved into: Reticulocyte-binding protein homolog 1 240 kDa form; Reticulocyte-binding protein homolog 1 120 kDa form] (P86148)
Organism: Plasmodium falciparum (isolate 3D7)
Function: During the asexual blood stage, binds to a sialic acid containing receptor on the surface of the host erythrocyte and thus is involved in merozoite invasion. Binds erythrocytes via a neuraminidase sensitive and trypsin-, chymotrypsin-resistant receptor. After merozoite attachment and reorientation, RH1 binding to its erythrocyte receptor triggers an increase in intracellular Ca(2+) within the parasite resulting in the release of microneme proteins such as EBA175 which in turn leads to the formation of the tight junction between parasite and host cell.
Subcellular location: [Reticulocyte-binding protein homolog 1]: Cell membrane {ECO:0000305|PubMed:12228308}; Single-pass type I membrane protein {ECO:0000305}. Note=Localizes to apical organelles in the maturing schizont and then translocates to the apical surface of the merozoite. {ECO:0000269|PubMed:12228308}.; SUBCELLULAR LOCATION: [Reticulocyte-binding protein homolog 1 240 kDa form]: Secreted {ECO:0000269|PubMed:19614665}. Cell junction, tight junction {ECO:0000269|PubMed:19614665}. Cytoplasmic vesicle, secretory vesicle, rhoptry {ECO:0000269|PubMed:19614665, ECO:0000269|PubMed:32452132}. Note=Localized to the rhoptry neck at the apical end of the merozoite (PubMed:19614665, PubMed:32452132). During merozoite invasion, mainly localizes to the tight junction formed between the parasite and the host erythrocytes membranes and then moves with the tight junction to the posterior end as the parasite enters the erythrocyte (PubMed:19614665). The remaining protein that does not participate in the tight junction, remains at the apical end of the merozoite and is incorporated into the newly formed ring stage (PubMed:19614665). {ECO:0000269|PubMed:19614665, ECO:0000269|PubMed:32452132}.; SUBCELLULAR LOCATION: [Reticulocyte-binding protein homolog 1 120 kDa form]: Cell membrane {ECO:0000269|PubMed:19614665}; Single-pass type I membrane protein {ECO:0000305}. Cell junction, tight junction {ECO:0000269|PubMed:19614665, ECO:0000269|PubMed:32452132}. Cytoplasmic vesicle, secretory vesicle, rhoptry {ECO:0000269|PubMed:19614665, ECO:0000269|PubMed:32452132}. Note=Localized to the rhoptry neck at the apical end of the merozoite (PubMed:19614665, PubMed:32452132). During merozoite invasion, mainly localizes to the tight junction formed between the parasite and the host erythrocytes membranes and then moves with the tight junction to the posterior end as the parasite enters the erythrocyte (PubMed:19614665). The remaining protein that does not participate in the tight junction, remains at the apical end of the merozoite and is incorporated into the newly formed ring stage (PubMed:19614665). {ECO:0000269|PubMed:19614665, ECO:0000269|PubMed:32452132}.
Interaction partners:
- Reticulocyte-binding protein homolog 5
- Reticulocyte-binding protein homolog 2b
Sequence:
MQRWIFCNIVLHILIYLAEFSHEQESYSSNEKIRKDYSDDNNYEPTPSYEKRKKEYGKDESYIKNYRGNNFSYDLSKNSSIFLHMGNGSNSKTLKRCNKKKNIKTNFLRPIEEEKTVLNNYVYKGVNFLDTIKRNDSSYKFDVYKDTSFLKNREYKELITMQYDYAYLEATKEVLYLIPKDKDYHKFYKNELEKILFNLKDSLKLLREGYIQSKLEMIRIHSDIDILNEFHQGNIINDNYFNNEIKKKKEDMEKYIREYNLYIYKYENQLKIKIQKLTNEVSINLNKSTCEKNCYNYILKLEKYKNIIKDKINKWKDLPEIYIDDKSFSYTFLKDVINNKIDIYKTISSFISTQKQLYYFEYIYIMNKNTLNLLSYNIQKTDINSSSKYTYTKSHFLKDNHILLSKYYTAKFIDILNKTYYYNLYKNKILLFNKYIIKLRNDLKEYAFKSIQFIQDKIKKHKDELSIENILQEVNNIYIKYDTSINEISKYNNLIINTDLQIVQQKLLEIKQKKNDITHKVQLINHIYKNIHDEILNKKNNEITKIIINNIKDHKKDLQDLLLFIQQIKQYNILTDHKITQCNNYYKEIIKMKEDINHIHIYIQPILNNLHTLKQVQNNKIKYEEHIKQILQKIYDKKESLKKIILLKDEAQLDITLLDDLIQKQTKKQTQTQTQTQKQTLIQNNETIQLISGQEDKHESNPFNHIQTYIQQKDTQNKNIQNLLKSLYNGNINTFIDTISKYILKQKDIELTQHVYTDEKINDYLEEIKNEQNKIDKTIDDIKIQETLKQITHIVNNIKTIKKDLLKEFIQHLIKYMNERYQNMQQGYNNLTNYINQYEEENNNMKQYITTIRNIQKIYYDNIYAKEKEIRSGQYYKDFITSRKNIYNIRENISKNVDMIKNEEKKKIQNCVDKYNSIKQYVKMLKNGDTQDENNNNNNDIYDKLIVPLDSIKQNIDKYNTEHNFITFTNKINTHNKKNQEMMEEFIYAYKRLKILKILNISLKACEKNNKSINTLNDKTQELKKIVTHEIDLLQKDILTSQISNKNVLLLNDLLKEIEQYIIDVHKLKKKSNDLFTYYEQSKNYFYFKNKKDNFDIQKTINKMNEWLAIKNYINEINKNYQTLYEKKINVLLHNSKSYVQYFYDHIINLILQKKNYLENTLKTKIQDNEHSLYALQQNEEYQKVKNEKDQNEIKKIKQLIEKNKNDILTYENNIEQIEQKNIELKTNAQNKDDQIVNTLNEVKKKIIYTYEKVDNQISNVLKNYEEGKVEYDKNVVQNVNDADDTNDIDEINDIDEINDIDEINDIDEINDIDEIKDIDHIKHFDDTKHFDDIYHADDTRDEYHIALSNYIKTELRNINLQEIKNNIIKIFKEFKSAHKEIKKESEQINKEFTKMDVVINQLRDIDRQMLDLYKELDEKYSEFNKTKIEEINNIRENINNVEIWYEKNIIEYFLRHMNDQKDKAAKYMENIDTYKNNIEIISKQINPENYVETLNKSNMYSYVEKANDLFYKQINNIIINSNQLKNEAFTIDELQNIQKNRKNLLTKKQQIIQYTNEIENIFNEIKNINNILVLTNYKSILQDISQNINHVSIYTEQLHNLYIKLEEEKEQMKTLYHKSNVLHNQINFNEDAFINNLLINIEKIKNDITHIKEKTNIYMIDVNKSKNNAQLYFHNTLRGNEKIEYLKNLKNSTNQQITLQELKQVQENVEKVKDIYNQTIKYEEEIKKNYHIITDYENKINDILHNSFIKQINMESSNNKKQTKQIIDIINDKTFEEHIKTSKTKINMLKEQSQMKHIDKTLLNEQALKLFVDINSTNNNLDNMLSEINSIQNNIHTYIQEANKSFDKFKIICDQNVNDLLNKLSLGDLNYMNHLKNLQNEIRNMNLEKNFMLDKSKKIDEEEKKLDILKVNISNINNSLDKLKKYYEEALFQKVKEKAEIQKENIEKIKQEINTLSDVFKKPFFFIQLNTDSSQHEKDINNNVETYKNNIDEIYNVFIQSYNLIQKYSSEIFSSTLNYIQTKEIKEKSIKEQNQLNQNEKEASVLLKNIKINETIKLFKQIKNERQNDVHNIKEDYNLLQQYLNYMKNEMEQLKKYKNDVHMDKNYVENNNGEKEKLLKETISSYYDKINNINNKLYIYKNKEDTYFNNMIKVSEILNIIIKKKQQNEQRIVINAEYDSSLINKDEEIKKEINNQIIELNKHNENISNIFKDIQNIKKQSQDIITNMNDMYKSTILLVDIIQKKEEALNKQKNILRNIDNILNKKENIIDKVIKCNCDDYKDILIQNETEYQKLQNINHTYEEKKKSIDILKIKNIKQKNIQEYKNKLEQMNTIINQSIEQHVFINADILQNEKIKLEEIIKNLDILDEQIMTYHNSIDELYKLGIQCDNHLITTISVVVNKNTTKIMIHIKKQKEDIQKINNYIQTNYNIINEEALQFHRLYGHNLISEDDKNNLVHIIKEQKNIYTQKEIDISKIIKHVKKGLYSLNEHDMNHDTHMNIINEHINNNILQPYTQLINMIKDIDNVFIKIQNNKFEQIQKYIEIIKSLEQLNKNINTDNLNKLKDTQNKLINIETEMKHKQKQLINKMNDIEKDNITDQYMHDVQQNIFEPITLKMNEYNTLLNDNHNNNINNEHQFNHLNSLHTKIFSHNYNKEQQQEYITNIMQRIDVFINDLDTYQYEYYFYEWNQEYKQIDKNKINQHINNIKNNLIHVKKQFEHTLENIKNNENIFDNIQLKKKDIDDIIININNTKETYLKELNKKKMLQNKKKVDEKSEINNHHTLQHDNQNVEQKNKIKDHNLITKPNNNSSEESHQNEQMKEQNKNILEKQTRNIKPHHVHNHNHNHNQNQKDSTKLQEQDISTHKLHNTIHEQQSKDNHQGNREKKQKNGNHERMYFASGIVVSILFLSSLGFVINSKNNKQEYDKEQEKQQQNDFVCDNNKMDDKSTQKYGRNQEEVMEISFDNDYI