Protein: Nuclear migration protein NUM1 (Q00402)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Controls nuclear migration. NUM1 specifically controls the interaction of the bud neck cytoskeleton with the pre-divisional G2 nucleus. Functions in dynein-anchoring. During late anaphase forms dynein-interacting cortical microtubule capture sites at both cellular poles. This leads to dynein-dependent sliding of the microtubules in the bud.
Subcellular location: Bud tip {ECO:0000269|PubMed:11121446, ECO:0000269|PubMed:11266443, ECO:0000269|PubMed:7490278}.
Interaction partners:
- Mitochondrial distribution and morphology protein 36
- Karyogamy protein KAR9
- Nuclear distribution protein PAC1
- Dynein heavy chain, cytoplasmic
- Nuclear migration protein JNM1
- Protein NIP100
- WD repeat-containing protein PAC11
- Nuclear distribution protein nudE homolog 1
- Kinesin-like protein KIP3
- Dynamin-related protein DNM1
Sequence:
MSHNNRHKKNNDKDSSAGQYANSIDNSLSQESVSTNGVTRMANLKADECGSGDEGDKTKRFSISSILSKRETKDVLPEFAGSSSHNGVLTANSSKDMNFTLELSENLLVECRKLQSSNEAKNEQIKSLKQIKESLSDKIEELTNQKKSFMKELDSTKDLNWDLESKLTNLSMECRQLKELKKKTEKSWNDEKESLKLLKTDLEILTLTKNGMENDLSSQKLHYDKEISELKERILDLNNENDRLLISVSDLTSEINSLQSNRTERIKIQKQLDDAKASISSLKRKVQKKYYQKQHTSDTTVTSDPDSEGTTSEEDIFDIVIEIDHMIETGPSVEDISEDLVKKYSEKNNMILLSNDSYKNLLQKSESASKPKDDELMTKEVAENLNMIALPNDDNYSKKEFSLESHIKYLEASGYKVLPLEEFENLNESLSNPSYNYLKEKLQALKKIPIDQSTFNLLKEPTIDFLLPLTSKIDCLIIPTKDYNDLFESVKNPSIEQMKKCLEAKNDLQSNICKWLEERNGCKWLSNDLYFSMVNKIETPSKQYLSDKAKEYDQVLIDTKALEGLKNPTIDFLREKASASDYLLLKKEDYVSPSLEYLVEHAKATNHHLLSDSAYEDLVKCKENPDMEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLEYLVEHAKATNHHLLSDSAYEDLVKCKENPDMEFLKEKSAKLGHTVVSNEAYSELQRKYSELEKEVEQPSLAYLVEHAKATDHHLLSDSAYEDLVKCKENPDVEFLKEKSAKLGHTVVSSEEYSELQRKYSELEKEVEQPSLAYLVEHAKATDHHLLSDSAYEELVKCKENPDMEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLAYLVEHAKATDHHLLSDSAYEDLVKCKENSDVEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLAYLVEHAKATDHHLLSDSAYEDLVKCKENPDMEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLEYLVEHAKATNHHLLSDSAYEDLVKCKENPDMEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLEYLVEHAKATNHHLLSDSAYEELVKCKENPDVEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLEYLVEHAKATNHHLLSDSAYEELVKCKENPDVEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLAYLVEHAKATDHHLLSDSAYEDLVKCKENPDVEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLAYLVEHAKATDHHLLSDSAYEDLVKCKENPDMEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLEYLVEHAKATNHHLLSDSAYEDLVKCKENPDMEFLKEKSAKLGHTVVSNKEYSELEKKLEQPSLEYLVKHAEQIQSKIISISDFNTLANPSMEDMASKLQKLEYQIVSNDEYIALKNTMEKPDVELLRSKLKGYHIIDTTTYNELVSNFNSPTLKFIEEKAKSKGYRLIEPNEYLDLNRIATTPSKEEIDNFCKQIGCYALDSKEYERLKNSLENPSKKFIEENAALLDLVLVDKTEYQAMKDNASNKKSLIPSTKALDFVTMPAPQLASAEKSSLQKRTLSDIENELKALGYVAIRKENLPNLEKPIVDNASKNDVLNLCSKFSLVPLSTEEYDNMRKEHTKILNILGDPSIDFLKEKCEKYQMLIISKHDYEEKQEAIENPGYEFILEKASALGYELVSEVELDRMKQMIDSPDIDYMQEKAARNEMVLLRNEEKEALQKKIEYPSLTFLIEKAAGMNKILVDQIEYDETIRKCNHPTRMELEESCHHLNLVLLDQNEYSTLREPLENRNVEDLINTLSKLNYIAIPNTIYQDLIGKYENPNFDYLKDSLNKMDYVAISRQDYELMVAKYEKPQLDYLKISSEKIDHIVVPLSEYNLMVTNYRNPSLSYLKEKAVLNNHILIKEDDYKNILAVSEHPTVIHLSEKASLLNKVLVDKDDFATMSRSIEKPTIDFLSTKALSMGKILVNESTHKRNEKLLSEPDSEFLTMKAKEQGLIIISEKEYSELRDQIDRPSLDVLKEKAAIFDSIIVENIEYQQLVNTTSPCPPITYEDLKVYAHQFGMELCLQKPNKLSGAERAERIDEQSINTTSSNSTTTSSMFTDALDDNIEELNRVELQNNEDYTDIISKSSTVKDATIFIPAYENIKNSAEKLGYKLVPFEKSNINLKNIEAPLFSKDNDDTSVASSIDLDHLSRKAEKYGMTLISDQEFEEYHILKDNAVNLNGGMEEMNNPLSENQNLAAKTTNTAQEGAFQNTVPHNDMDNEEVEYGPDDPTFTVRQLKKPAGDRNLILTSREKTLLSRDDNIMSQNEAVYGDDISDSFVDESQEIKNDVDIIKTQAMKYGMLCIPESNFVGASYASAQDMSDIVVLSASYYHNLMSPEDMKWNCVSNEELQAEVKKRGLQIALTTKEDKKGQATASKHEYVSHKLNNKTSTVSTKSGAKKGLAEAAATTAYEDSESHPQIEEQSHRTNHHKHHKRQQSLNSNSTSKTTHSSRNTPASRRDIVASFMSRAGSASRTASLQTLASLNEPSIIPALTQTVIGEYLFKYYPRLGPFGFESRHERFFWVHPYTLTLYWSASNPILENPANTKTKGVAILGVESVTDPNPYPTGLYHKSIVVTTETRTIKFTCPTRQRHNIWYNSLRYLLQRNMQGISLEDIADDPTDNMYSGKIFPLPGENTKSSSKRLSASRRSVSTRSLRHRVPQSRSFGNLR