Protein: Target of rapamycin complex 2 subunit MAPKAP1 (TORC2 subunit MAPKAP1) (Mitogen-activated protein kinase 2-associated protein 1) (Stress-activated map kinase-interacting protein 1) (SAPK-interacting protein 1) (Q8BKH7)
Organism: Mus musculus (Mouse)
Function: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Isoform 1 is involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.
Subcellular location: Cell membrane {ECO:0000250|UniProtKB:Q9BPZ7}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9BPZ7}. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9BPZ7}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9BPZ7}. Early endosome membrane {ECO:0000250|UniProtKB:Q9BPZ7}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9BPZ7}. Late endosome membrane {ECO:0000250|UniProtKB:Q9BPZ7}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9BPZ7}. Lysosome membrane {ECO:0000250|UniProtKB:Q9BPZ7}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9BPZ7}. Golgi apparatus membrane {ECO:0000250|UniProtKB:Q9BPZ7}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9BPZ7}. Mitochondrion outer membrane {ECO:0000250|UniProtKB:Q9BPZ7}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9BPZ7}. Cytoplasm, perinuclear region {ECO:0000305|PubMed:30837283}. Nucleus {ECO:0000250|UniProtKB:Q9BPZ7}. Note=The mTORC2 complex localizes to membranes: mTORC2 is active at the plasma membrane, endoplasmic reticulum membrane, lysosomes and perinuclear region (By similarity). Iin lysosomal membrane, mTORC2 is sensitive to lysosomal positioning in the cell (By similarity). Following phosphorylation by PKC, localizes to the perinuclear region, where the mTORC2 complexe specifically phosphorylates SGK1, but not AKT (PubMed:30837283). {ECO:0000250|UniProtKB:Q9BPZ7, ECO:0000269|PubMed:30837283}.
Interaction partners:
- Serine/threonine-protein kinase mTOR
- Rapamycin-insensitive companion of mTOR
- DEP domain-containing mTOR-interacting protein
- Proline-rich protein 5
- Proline-rich AKT1 substrate 1
- Target of rapamycin complex subunit LST8
- TELO2-interacting protein 1 homolog
- Regulatory-associated protein of mTOR
- Proline-rich protein 5-like
- Telomere length regulation protein TEL2 homolog
Sequence:
MAFLDNPTIILAHIRQSHVTSDDTGMCEMVLIDHDVDLEKTHPPSVPGDSGSEVQGSSGETQGYIYAQSVDITSSWDFGIRRRSNTAQRLERLRKERQNQIKCKNIQWKERNSKQSAQELKSLFEKKSLKEKPPSSGKQSILSVRLEQCPLQLNNPFNEYSKFDGKGHVGTTATKKIDVYLPLHSSQDRLLPMTVVTMASARVQDLIGLICWQYTSEGREPKLNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHKFGFSTLALVEKYSSPGLTSKESLFVRINAAHGFSLIQVDNTKVTMKEILLKAVKRRKGSQKISGPQYRLEKQSEPNIAVDLESTLESQNAWEFCLVRENSSRADGVFEEDSQIDIATVQDMLSSHHYKSFKVSMIHRLRFTTDVQLGISGDKVEIDPVTNQKASTKFWIKQKPISIDCDLLCACDLAEEKSPSHAVFKLTYLSSHDYKHLYFESDAATVSEIVLKVNYILESRASTARADYLAQKQRKLNRRTSFSFQKEKKSGQQ