Protein: Separin homolog sep-1 (EC 3.4.22.49) (Separase) (G5ED39)
Organism: Caenorhabditis elegans
Function: Cysteine protease, which plays a central role in homologous chromosome separation during meiosis I and in sister chromatid separation during embryonic mitosis. Promotes chromosome/sister chromatid segregation by cleaving the scc-1 (mitosis) and rec-8 (meiosis) subunits of the cohesin complex at the onset of anaphase (Probable). May cleave histone H3-like protein cpar-1 during meiosis I metaphase-anaphase transition. Promotes cortical granule exocytosis after oocyte fertilization during the first meiotic anaphase. Essential for embryonic cytokinesis by regulating rab-11-positive vesicle trafficking at the plasma membrane at the cleavage furrow and midbody. Regulates centriole segregation during spermatocyte meiosis. Required for embryonic anterior-posterior axis formation.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:17913784, ECO:0000269|PubMed:23401519}. Chromosome {ECO:0000269|PubMed:17913784, ECO:0000269|PubMed:20116245, ECO:0000269|PubMed:23401519}. Cytoplasmic granule {ECO:0000269|PubMed:17913784, ECO:0000269|PubMed:21878498}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:17913784}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:17913784, ECO:0000269|PubMed:20116245, ECO:0000269|PubMed:23401519}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:17913784, ECO:0000269|PubMed:20116245, ECO:0000269|PubMed:21878498}. Cleavage furrow {ECO:0000269|PubMed:20116245}. Midbody {ECO:0000269|PubMed:20116245}. Note=Before ovulation, accumulates on chromosomes and cortical filaments in oocytes (PubMed:17913784). By metaphase I, dissociates from filaments and transiently localizes to cortical granules until their exocytosis during anaphase I followed by an accumulation at the cortex near the polar body (PubMed:17913784). Localizes to meiotic spindle matrix (PubMed:21878498). During mitotic metaphase and anaphase, localizes to chromosomes, centrosomes and the spindle matrix (PubMed:17913784, PubMed:20116245, PubMed:21878498). During spermatogenesis, localizes to the spermatocyte cytoplasm throughout meiotic prophase I (PubMed:23401519). At the diplotene stage of prophase I accumulates at low levels in puncta around the nuclear envelope (PubMed:23401519). At diakinesis, transiently localizes to centrosomes until nuclear envelope breakdown (PubMed:23401519). At metaphase I and II becomes highly enriched around chromosomes (PubMed:23401519). At anaphase I and II, chromosomal localization is reduced and centrosome localization is highly increased (PubMed:23401519). Localizes to residual body during the budding division leading to spermatid formation (PubMed:23401519). {ECO:0000269|PubMed:17913784, ECO:0000269|PubMed:20116245, ECO:0000269|PubMed:21878498, ECO:0000269|PubMed:23401519}.
Interaction partners:
- Securin-like protein
- Structural maintenance of chromosomes protein 3
- Serine/threonine-protein kinase plk-1
- Cyclin N-terminal domain-containing protein
- G2/mitotic-specific cyclin-B
- Cyclin-dependent kinase 1
- Mitotic checkpoint serine/threonine-protein kinase bub-1
- Serine/threonine-protein kinase plk-2
- Mitotic checkpoint protein bub-3
- Serine/threonine-protein kinase plk-3
Sequence:
MKITNKSVDKQHIEKLDELRKNVSCTVIGFAEQTAELQQEISELFIAEFGVNGPIDMNSLSKLARITSYYASSEYFQGLAKYQRTACKMFITWQTLRKEAMECRSKDREIFASIPAKLCFFYFYNGELCRAVVCLLDYIDLSDDTLAKEAALRWLMFLGETELIEKKLKTWKMDKSSKDMFSATEFAMNYLKKSEYRVEMLEKLMKLRDKVKSDPTRSFSRYELASYVSWLCSTLSNVPVGSALRECEFPDRVSHIQEAALKSDSLVRNRIPGLASSQFDNSVNASIWPFLDGHQEDSNYYVHIGSTIAWHFEMRRECALVNVTTAQTRDSMSAMILNLRVALKSASFFRVLQTTNTLAYYSSIIEEAGSEKNAKLMRVSCVNLLSSNPIIVRCSTPKETGATSRAHTPMAGSSVSEKQNTMRPDLADLLGDLELLDEQSFHPITRSCVCNVCTIYPLHSSFAAEYMMSYAIHSDFSQLSIKHFNDEFARIRERGMSSQVLMHRDSSVRPRPNIIQNEIFGMCVIRWLTKKLDSKESADEDTMEIFNNALKIVRYLQQRTTDMILAVTQLGRQLEFPMECNYSWMRPTIRKPRVKATIDCAVDILRAVSPFGRRPKVEKLEKNLQPFDKERFEKVRLAMRNEMNHYGHILYREWRCRLFAYVGRTSRDPWEAAYAWAESTQIGARNAVQSRLEKCKRGLVTMSGHDRFKTCVQSMPDEMTLVQIAMADDKTIYLVKLHADRDPIIMPLAHYSQAVELMDKFTFLLDEDEMIAKYPGDITPEEFWKRRKIVDGRMMTFVDEVQKHFLGVAASLLMPSGQLGPKAAELAIKIHKLSKGGLLLGEAKEMVYQSKLMDAKSWEALILRFCEMRTTDEKFKSFLPLMHRNSVEVMNQDDSIVTEKKYTYLVICPHLSQFCWERLPIFDEYPYVGRQVSIHSTFSQLEAMKSQEKQIPLQIDVQNAYYILDPDNNLGETQKRMVEYINKFNWEGTVGSAPKSNEISAALSQRDAFFFIGHGSGSSVMPRSVLKQSTCNAISLLMGCGSVRTIPQALGFDGKTAILDYAMAKCPLIVGCLWTVTDGEIDRFLIRMIDDCFEDSKSLTGIDKLRQLSEAMHEARSKARLKYLTGAAVVMYGLPVVAKQTTPFVEKDQRNLPQTPKTSARTSMRMETVPKTPKQEFVTSKSVPMTPIFSNNENKSPSRARMPSRVLKTPRQVKTFQEEDDEAPKRSTTRQLKPLVAPPIPATPTTRTTRSSARTPSRSRNL