Protein: Zip homologous protein 3 (Zip3-homologous protein) (C6KRL6)
Organism: Caenorhabditis elegans
Function: Recruited co-dependently with zhp-4 to the synaptonemal complex between homologous chromosome pairs to regulate the formation and number of crossover events between homologs during meiotic recombination. In the early stages of pachytene, in complex with zhp-4, recruited by the zhp-1-zhp-2 heterodimer to designated crossover sites along the homolog pair to stabilize other pro-crossover factors such as rmh-1, msh-5 and cosa-1. This in turn facilitates crossover and promotes the formation of chiasma in each meiotic nucleus at the late pachytene stage of meiosis. Plays a role in the segregation of homologous chromosomes following the completion of crossovers. Together with him-14 and msh-5 plays a role in the activation of DNA damage-dependent apoptosis at the DNA damage checkpoint in pachytene cells.
Subcellular location: Chromosome {ECO:0000269|PubMed:15340062, ECO:0000269|PubMed:18949042, ECO:0000269|PubMed:29521627, ECO:0000269|PubMed:30379819}. Note=Co-localizes with zhp-4 to chromosomes from mitosis to early diakinesis in the germline (PubMed:29521627, PubMed:30379819). Co-localizes with syp-1, a component of the synaptonemal complex, throughout the gonad from early prophase to mid-pachytene (PubMed:15340062, PubMed:18949042, PubMed:29521627, PubMed:30379819). In early pachytene, co-localizes with syp-1 as puncta along chromosomes (PubMed:18949042). In pachytene nuclei, localizes in linear arrays in the space in between synapsed chromosomes (PubMed:15340062). Does not localize to unsynapsed chromosomes (PubMed:18949042). From mid-pachytene, co-localizes with cosa-1 at crossover sites of recombination intermediates, and gradually disassociates from syp-1 along both chromosome arms (PubMed:15340062, PubMed:18949042, PubMed:29521627, PubMed:30379819). Co-localizes with brc-1 at crossover sites in mid-late pachytene nuclei (PubMed:30383754). At late pachytene, localizes asymmetrically on synapsed chromosomes (PubMed:18949042). At late pachytene and early diplotene localizes to a single focus at the boundary between the long and short arm of each pair of homologous chromosomes (PubMed:18949042). At late pachytene, localization at chromosomes is not dependent on syp-1 (PubMed:18949042, PubMed:29521627). In diakinesis, does not localize to chromosomes, but is dispersed between chromosomes (PubMed:15340062). {ECO:0000269|PubMed:15340062, ECO:0000269|PubMed:18949042, ECO:0000269|PubMed:29521627, ECO:0000269|PubMed:30379819, ECO:0000269|PubMed:30383754}.
Interaction partners:
- Meiotic recombination protein spo-11
- MutS protein homolog 5
- Cyclin N-terminal domain-containing protein
- MutS protein homolog him-14
- Transcription factor zip-3
- HORMA domain-containing protein
- Biogenesis of lysosome-related organelles complex 1 subunit 1
- HORMA domain-containing protein
- Zip homologous protein 4
- Long arms of the bivalent protein 1
Sequence:
MDFVHCNKCFNRKPPDGFFISSCFHIFCTKCAKADLAVCLICKKNVRLVRLDGNISSGIKIYFADPIKMVADSLAKIQKKIDFQQSTRDHLVKYLTKEKEKKRQMEVYFRTKGQEFDSQRKKLAEATAWIQMAEKKLQASEEERVKAEREIEECQAKLKSMTNLMSADTLGMNSQTPFPFSLAESQETAPSLVESSANSTFNMVSPLVSSPASSPNSINYNSFFENGSRTRPESLNEEAMFNTMLQSSGQSANANTSESSAFSVAFNNIFTPSRNNMGDSSMINKTTANQTIMDKTSMSLENWRQNRANSFGVHDISKRDSSLPTGGGSAIRVHHFKQNSRITPIAQNRRSAAGFDRQQIQEMRRISSQPGYLAQRKPINGRSFIGPAD