Protein: Integrin beta pat-3 (Paralyzed arrest at two-fold protein 3) (Q27874)
Organism: Caenorhabditis elegans
Function: Integrin alpha ina-1/beta pat-3 is a receptor for laminin. Integrin alpha pat-2/beta pat-3 recognizes the sequence R-G-D in its ligands (Probable). Plays a role in cell migration, morphogenesis and probably in cell-cell interactions. During gonad morphogenesis, involved in distal tip cell (DTC)-mediated guidance of gonad elongation, in maintaining their sharp tapering morphology and in their migration. Component of an integrin containing attachment complex, which is required for muscle development and maintenance. Involved in the assembly of dense bodies and M lines during body wall muscle embryonic development by recruiting one of their components, cpna-1, to integrin-mediated attachment sites. May play a similar role in the assembly of dense bodies in gonadal myoepithelial sheath cells. Probably by acting as a receptor for apoptotic cells, plays a role in the clearance of apoptotic cells during mid-embryogenesis. Required for ovulation. Dephosphorylated, probably within the alpha pat-2/beta pat-3 integrin receptor complex, by the phosphatase dep-1, which leads to down-stream effects including the negative regulation of let-23 signaling and vulval induction. When unphosphosphorylated, recruits the cytoplasmic adapter protein tln-1 to the plasma membrane of secondary vulval precursor cells. This promotes the linking of focal adhesion sites to the F-actin cytoskeleton, and it also acts to restrict the mobility of the let-23 receptor on the plasma membrane of vulval cells which thereby attenuates let-23 signaling. Plays a role in axon regeneration after injury.
Subcellular location: Cell membrane {ECO:0000269|PubMed:9247263}; Single-pass type I membrane protein {ECO:0000305}. Lateral cell membrane {ECO:0000269|PubMed:28135265}; Single-pass type I membrane protein {ECO:0000305}. Basolateral cell membrane {ECO:0000269|PubMed:28135265, ECO:0000269|PubMed:32053105}; Single-pass type I membrane protein {ECO:0000305}. Cytoplasm, myofibril, sarcomere, M line {ECO:0000269|PubMed:22479198, ECO:0000269|PubMed:23283987}. Cell junction, focal adhesion {ECO:0000269|PubMed:20385102}. Note=In body wall muscle cells localizes to the base of thick filament M-lines, dense bodies (Z-disks) and in adhesion plaques which form between adjacent cells (PubMed:20385102, PubMed:22479198). In body wall muscles, colocalizes with cpna-1 at integrin adhesion structures (M line and dense bodies) (PubMed:23283987). Decreased localization to dense bodies during the L2/L3 molt (PubMed:20385102). In myoepithelial sheath cells, colocalizes in dense bodies-like structures with actin thin filaments, deb-1/vinculin and unc-52 (PubMed:17326220). {ECO:0000269|PubMed:17326220, ECO:0000269|PubMed:20385102, ECO:0000269|PubMed:22479198, ECO:0000269|PubMed:23283987}.
Interaction partners:
- Integrin alpha ina-1
- Integrin alpha pat-2
- Integrin-linked protein kinase homolog pat-4
- FERM domain-containing protein
- Vinculin
- Protein unc-112
- Paxillin homolog 1
- Tensin homolog
- Laminin-like protein lam-2
- Laminin-like protein epi-1
Sequence:
MPPSTSLLLLAALLPFALPASDWKTGEVTGKVVEKSEFPCYSLSRDNYTCSACIQYHESCAWCGAPMFDEKKPYARCDSRAKLMEHGCPNSYIEDPATKLDITEDSKLSDQGQVESEEEAVQIKPQEMYVEIRPKSRVRFNVTYRQAVDYPVDLYYLMDLSYSMKDDKQKLSELGDLLAERMRTVTKNFRLGFGSFIDKKLMPFIDPRIEKQLSPCPTPCAEPYGFKHQMSLTTNTAKFKAEVDKAEISGNLDAPEGGFDAVVQALACNKTIGWRERARKMIVFSTDAGFHFAGDGRLAGVVEPNDGTCHLDREGYYTETLNQDYPSIALLHQMIKDRKANVIFAVTKNNQDLYTQLSNALPDVSSSVGVLANDSRNIVDLIEKEYLKISEKIIMVDNANASEGLKLTYRSMCLDGTTLKDTNVCEGIRVGDEVQFEVTLENTHCIDKRDFVLRIGPSGLDETLIVNVKVLCDCDCERQDRIVTNSADCNGGDMVCGVCRCKGGNVGKYCECNRPGMSTAALNEKCKRTNESAICEGRGVCNCGRCECNPRANPEEQISGEFCECDNFNCPRHDRKICAEHGECNCGKCICAPGWTGRACECPISTDSCLSANGKICNGKGECICGRCRCFDSPDGNRYSGAKCEICPTCPTKCVEYKNCVMCQQWQTGPLNETACDQCEFKVIPVEELPNLNETTPCQFVDPADDCTFYYLYYYDEATDNATVWVRKHKDCPPPVPVLAIVLGVIAGIVILGILLLLLWKLLTVLHDRSEYATFNNERLMAKWDTNENPIYKQATTTFKNPVYAGKAN