Protein: Hypoxia-inducible factor prolyl hydroxylase (HIF-PH) (EC 1.14.11.29) (Egg-laying defective protein 9) (Hypoxia-inducible factor-proline dioxygenase) (G5EBV0)
Organism: Caenorhabditis elegans
Function: Cellular oxygen sensor which regulates the stability and the activity of hypoxia-inducible transcription factor, hif-1. In normoxic conditions, hydroxylates hif-1 targeting it for vhl-1-mediated proteasomal degradation. In addition, regulates hif-1 transcriptional activity in a vhl-1-independent manner and independently of its hydroxylase activity. By regulating hif-1 activity, controls several cellular responses. Mediates susceptibility to B.thuringiensis and V.cholerae pore-forming toxins and enteropathogenic E.coli. Mediates susceptibility to P.aeruginosa PAO1-mediated killing by regulating resistance to cyanide produced by P.aeruginosa. Mediates resistance to S.aureus-mediated killing. In addition, plays a role in heat acclimation, neuronal development, behavioral responses to reoxygenation and hydrogen sulfide, iron homeostasis and aging. In neurons, involved in mitochondrion fusion during reoxygenation. Involved in egg laying.
Subcellular location: [Isoform a]: Cytoplasm {ECO:0000269|PubMed:22252129}. Nucleus {ECO:0000269|PubMed:22252129}. Cell projection, dendrite {ECO:0000269|PubMed:22252129}. Note=In interneurons, localizes throughout the ventral cord dendrites. {ECO:0000269|PubMed:22252129}.; SUBCELLULAR LOCATION: [Isoform c]: Cytoplasm {ECO:0000269|PubMed:22252129}. Nucleus {ECO:0000269|PubMed:22252129}. Cell projection, dendrite {ECO:0000269|PubMed:22252129}. Note=In interneurons, localizes throughout the ventral cord dendrites. {ECO:0000269|PubMed:22252129}.; SUBCELLULAR LOCATION: [Isoform e]: Cytoplasm {ECO:0000269|PubMed:22252129}. Nucleus {ECO:0000269|PubMed:22252129}. Cell projection, axon {ECO:0000269|PubMed:22252129}. Note=In interneurons, localizes in puncta (which probably correspond to endosomes) along the ventral cord. Localization to puncta is regulated by oxygen levels. Co-localizes with lin-10 in puncta. {ECO:0000269|PubMed:22252129}.
Interaction partners:
- Hypoxia-inducible factor 1
- von Hippel-Lindau tumor suppressor homolog
- Cysteine synthase 1
- Fe2OG dioxygenase domain-containing protein
- Regulator of hypoxia-inducible factor 1
- Aryl hydrocarbon receptor nuclear translocator homolog
- Nuclear hormone receptor family member nhr-57
- Prolyl 4-hydroxylase subunit alpha-2
- Fe2OG dioxygenase domain-containing protein
- Bifunctional L-3-cyanoalanine synthase/cysteine synthase
Sequence:
MSSAPNDDCEIDKGTPSTASLFTTLMLSQPSSSTAVLQCTYCGSSCTSSQLQTCLFCGTVAYCSKEHQQLDWLTHKMICKSLQTSGMVPSNLMPQAAPAVMAPIPPTVSFDDPALTTSLLLSLQNNPILNQTISNFPPTFSITSKTEPEPSIPIQIPQRISSTSTVPFSSEGSAFKPYRNTHVFNSISSESMSSMCTSHEASLEHMSSASLAMFPTSSTAQSDISRLAQVLSLAGDSPASLALVTTSVPSTASTATIPPPATTTSSATSSGKSETITVGKEKIIQTDDPDIQIIETEGGSKPTVSRTRKRPTPSNSADPKINYKDHNKNVVYSTTLQEHQKHLQNRGLALSIHQAMVLRLRYIAEHVIRSLNEFGWAVVDNFLGSDHYKFTAKEIERLYERGLFSPGQLMEAKHKDEFHIKDIRSDHIYWYDGYDGRAKDAATVRLLISMIDSVIQHFKKRIDHDIGGRSRAMLAIYPGNGTRYVKHVDNPVKDGRCITTIYYCNENWDMATDGGTLRLYPETSMTPMDIDPRADRLVFFWSDRRNPHEVMPVFRHRFAITIWYMDKSERDKALAKGKESDAACASKKENDPTSSSLNSLIGSLLRPRKNPSTHDLSKLDLRLFPSTSSDPALVSAADEDRVDISADFQSTSSLAHPESTDSGVSLSTFNVAHNHMERTTSLQSISDHFRSERSHERRSSTSSDQDLDEGLPPPPSTNPEYYI