Protein: Centrosome-associated zinc finger protein Cp190 (Centrosomal protein 190kD) (Microtubule associated protein 190kD) (Protein enhancer of mod(mdg4)4-1) (Q24478)
Organism: Drosophila melanogaster (Fruit fly)
Function: Component of the gypsy chromatin insulator complex which is required for the function of the gypsy chromatin insulator and other endogenous chromatin insulators. Chromatin insulators are regulatory elements which establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops. This chromatin looping may involve the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein may directly bind to insulator DNA at sites distinct from those recognized by su(Hw). Required during embryogenesis for axial expansion, an actin/myosin dependent process that distributes the dividing nuclei along the anterior-posterior axis of the syncytial embryo. Does not appear to play a crucial role in organizing centrosomal microtubules during mitosis.
Subcellular location: Nucleus {ECO:0000269|PubMed:14996941, ECO:0000269|PubMed:25342723, ECO:0000269|PubMed:26649574}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:14996941, ECO:0000269|PubMed:26649574, ECO:0000269|PubMed:8491775, ECO:0000269|PubMed:8522588, ECO:0000269|PubMed:8590797}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:14996941, ECO:0000269|PubMed:26649574}. Chromosome {ECO:0000269|PubMed:25342723, ECO:0000269|PubMed:26649574, ECO:0000269|PubMed:38769058}. Nucleus, nucleoplasm {ECO:0000269|PubMed:38769058}. Note=Localizes to nucleus during interphase and to the centrosome during mitosis (PubMed:25342723, PubMed:26649574). Colocalizes with other elements of the gypsy chromatin insulator complex at multiple sites on polytene chromosomes and at nuclear insulator bodies (PubMed:25342723). On polytene chromosomes of third-instar larvae colocalizes with pita and ZIPIC (PubMed:25342723). Predominantly present in the nucleoplasm (PubMed:38769058). Localizes to spindle microtubules during mitosis (PubMed:26649574). {ECO:0000269|PubMed:25342723, ECO:0000269|PubMed:26649574, ECO:0000269|PubMed:38769058}.
Interaction partners:
- Zinc finger protein su
- Modifier of mdg4
- CTCF
- Boundary element associated factor
- LD02709p
- Insulator binding factor 1, isoform A
- Insulator binding factor 2
- Chromator, isoform A
- Zinc finger protein ZIPIC
- Deformed wings
Sequence:
MGEVKSVKVDNWGVFFLQKLQNFFNKTDYCDLTLQFRDNSQLKVHRLVLSACTDYFNVLEQTCEIVDDALIMPNEFQADVVVPIVNFMYTGTLEFELKMYGKLLRTAKEMNMTVLLKLLEAHRRTMENVNRQQRPPSPKGIRRRTVGQPSSGLPQQRVLGPSPQSRNVATPIAQRANTQRGSTGNTMSRTSGGSNRSPYGDSSNVKQEPTSPFEQLRKGYNNNKRPAQTSLLSPPSKKPSLEEVKEFAEQQRMRKQIAAEYGDNDPEYDGGMLYDDVHAGDDDDDDMPPQPSTSKQQSPQGTQTQLEHGSTTIILKQDSPSQTPTIIVKDSSNAKLNHTKIIAEVLRQYPHIVKGHKNIKLKIMPNTPAAPTEKSAPATVKPPANQSSATTSPHKKLHVSFKADKSTPLITAQQKAASSQQKSGTSQTTGNQGTGANPPANTAAAQKRRIDSKTMHALIAQGAENTTGPWLCLRCGVNGRPISIPSYRGFRRHLINTHKETIDPALCEHCGWRSVNNRELHFHMYMEHQTKSLLYTFAECALCNQSYRTKGELEAHINEVHTDDNKQQCIYCNKVFEQELQLYRHMKSYHKEQALEDGIIDETDEEFLGSQDEEEEAEGDEEQEPEQTGKVRILSDISLPATSAITVQQAQQEQLQEEDVEQVQQEVKFVGADGNEVELTDEQRKEILSQLNQQQAGATAGGVVMVLSEPEAEHVKQETDEKSLAGTEEEYDDSQIYSELGAADSVESAKKNIADESKESIDNLEWAENLIAESEEQSNKEPKSDKPRDDISEKLKELTGDWTEDENDDDVDDKPATAELASELANKDPEPTVHEEEDDIDLALQSLHKGPEEATEEKASEESVTSADDAVDAVPNINSQPEKMDVDSEAADEKASKAEVQIKKEAELENDQEEFIKEDSPIPHSDSVAELREAVTASEGEDDVHLEADNIRKELLDELIAEAEKPDQEKDIVQSEENATTEALDRSVTDEDDLVPPTQVSTEQMEIDEPAAEKAAENNEDTRTADEKEAVEDKPNQTQDVTTAEKPTLESAKAGDEATSGEAASVDKVKSLISEWGDDDEDEDENGVSAAAKEEL