Protein: RNA-directed RNA polymerase L (Protein L) (EC 2.7.7.48) (Large structural protein) (Replicase) (Transcriptase) [Includes: cap-snatching endonuclease (EC 3.1.-.-)] (Q6IUF8)
Organism: Machupo virus (MACV)
Function: RNA-dependent RNA polymerase, which is responsible for the replication and transcription of the viral RNA genome using antigenomic RNA as an intermediate. During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, which involves the endonuclease activity cleaving the host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner.
Subcellular location: Virion {ECO:0000255|HAMAP-Rule:MF_04086}. Host cytoplasm {ECO:0000255|HAMAP-Rule:MF_04086}.
Interaction partners:
Sequence:
MDEYVQELKGLIRKHIPDRCEFAHQKVTFLSQVHPSPLLTEGFKLLSSLVELESCEAHACQANTDQRFVDVILSDNGILCPTLPKVIPDGFKLTGKTLILLETFVRVNPDEFEKKWKADMSKLLNLKHDLQKSGVTLVPIVDGRSNYNNRFVADWVIERMRWLLIEILKASKSMLEIDIEDQEYQRLIHSLSNVKNQSLGLENLEHLKRNSLDYDERLNESLFIGLKGDIRESTVREELIKLKMWFKDEVFSKGLGKFKLTDRRELLESLSSLGAHLDSDVSSCPFCNNKLMEIVYNVTFSSVERTDGAATVDQQFSTTHTNIEKHYLSVLSLCNKIKGLKVFNTRRNTLLFLDLIMVNLMVDISESCQDAIESLRKSGLIVGQMVMLVNDRVLDILEAIKLIRKKIGTNPNWVKNCSKILERSHPEIWLQLNTLIRQPDFNSLISIAQYLVSDRPIMRYSVERGSDKICRHKLFQEMSSFEQMRLFKTLSSISLSLINSMKTSFSSRLLVNEREFSKYFGNVRLRECYAQRFYLAESLVGFLFYQKTGERSRCYSVYLSDNGVMSEQGSFYCDPKRFFLPVFSDEVLAGMCEEMTSWLDFDTGLMNDTGPILRLLVLAILCSPSKRNQTFLQGLRYFLMAFANQIHHIDLISKLVVECKSSSEVVVQRLAVGLFIRLLGGESDASSFFSRRFKYLLNVSYLCHLITKETPDRLTDQIKCFEKFIEPKVKFGCAVVNPSLNGKLTVDQEDIMINGLKKFFSKSLRDTEDVQTPGVCKELLNYCVSLFNRGKLKVSGELKNNPFRPNITSTALDLSSNKSVVIPKLDELGNILSTYDKEKLVSACVSSMAERFKTKGRYNLDPESTDYLILKNLTGLVSAGPKAKSSQEELSLMYETLTEEQVESFNEIKYDVQVALAKMADNSVNTRIKNLGRADNSVKNGNNPLDNLWSPFGVMKEIRAEVSLHEVKDFDPDVLPSDVYKELCDAVYKSSEKCNFFLEEVLDVCPLGLLLKNLTTSSYMEEEYFMCFKYLLIQGHFDQKLGSYEHKSRSRLGFTDETLRLKDEVRLSIRESNSEAIADKLDKSYFTNAALRNLCFYSEDSPTEFTSISSNSGNLKFGLSYKEQVGSNRELYVGDLNTKLMTRLVEDFSEAVGNSMKYTCLNSEKEFERAICDMKMAVNNGDLSCSYDHSKWGPTMSPALFLALLQMLELRTPVDRSKIDLDSVKSILKWHLHKVVEVPINVAEAYCIGKLKRSLGLMGCGSTSLSEEFFHQTMQLSGQIPSHIMSVLDMGQGILHNTSDLYGLITEQFLCYALDLLYDVIPVSYTSSDDQITLVKTPSLDIEGGSDAAEWLEMICFHEFLSSKLNKFVSPKSVIGTFVAEFKSRFFVMGEETPLLTKFVSAALHNVKCKTPTQLSETIDTICDQCIANGVSTKIVARISKRVNQLIRYSGYGDTPFGAIEDQDVKDWVDGSRGYRLQRKIEAIFYDDKETSFIRNCARKVFNDIKRGRIFEENLINLIGRGGDEALTGFLQYAGCSEQEVNRVLNYRWVNLSSFGDLRLVLRTKLMTSRRVLEREEVPTLIKTLQSKLSRNFTKGVKKILAESINKSAFQSSVASGFIGFCKSMGSKCVRDGKGGFLYIKEVYSGINVCICEICALKPKIIYCNDSLNKVSQFSKPILWDYFSLVLTNACELGEWVFSTVKEPQKPLVLNNQNFFWAVKPKVVRQIEDQLGMNHVLQSIRRNYPVLFDEHLAPFMNDLQVSRTMDSGRLKFLDVCIALDMMNENLGIISHLLKTRDNSVYIVKQSDCALAHIRQSSYTDWELGLSPQQICTNFKTQLVLSSMVNPLVLSTSCLKSFFWFNEVLELEDDSQIELAELTDFALMVKNQNVSRAMFVEDIAMGYVVSNFEGVRISLSNVMVDGVQLPPKEKAPDVGVLFGLKAENVIVGLVVQIDHVRMSTKFKLRRKMVYSFSLECTMDVGDIQNKEVILKVVAVDQSVSGSGGNHMLLDGVPVIASLPLFTGQASFDLAAMLIESNLAGSNDNFLMSNVTLDLGGFSPELSDKYSYRLSGPENQEDPLVLKDGAFYVGGERLSTYKVELTGDLVVKALGALEDDEGVVSMLHQLWPYLKATSQVILFQQEDFTIVHDLYKIQLTKSIESFGEWIEFTNFKVAYSKSLKELVISDTQGSFRLKGVMCRPLANTLQVEDIE