Protein: tRNA3(Ser)-specific nuclease WapA (EC 3.1.-.-) (Cell wall-associated protein) (RNase WapA) (Toxin WapA) (G4NYJ6)
Organism: Bacillus spizizenii (strain DSM 15029 / JCM 12233 / NBRC 101239 / NRRL B-23049 / TU-B-10) (Bacillus subtilis subsp. spizizenii)
Function: Toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. A site-specific tRNA3(Ser) nuclease, the C-terminus (residues 2201-2335) probably removes 2 or 4 nucleotides from the 3' end of tRNA3(Ser) but not tRNA2(Arg) or tRNA(Glu) (upon expression in E.coli), possibly endonucleolytically. The nuclease activity is neutralized by expression of the cognate immunity protein WapI from the same strain, but not its homolog from 2 other B.subtilis strains. The C-terminus cannot be expressed on its own in E.coli, however it can be cloned in the presence of its cognate immunity protein gene wapI. Cell contact is probably necessary for growth inhibition.
Subcellular location: Secreted, cell wall {ECO:0000250}. Note=Released into the medium. {ECO:0000250}.
Interaction partners:
- Two-component system YycF/yycG regulatory protein yycH
- LysM domain-containing protein
- YqgU
- Cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB
- TMP repeat family
- M protein repeat family protein
- Immunity protein WapI
- Putative flagellar hook-length control protein
- YclG
- YwqB
Sequence:
MKKRKRRTFKRFIAAFLVLSLMISLLPADVLAKTTEEEAGNRIVSDDPEETPRNEQTEEAVPFPSKDINKEGEITSERTENTKLYYEGDGVYKQEVYLDPIHTKETPNADWEDISPELKESTSKQVETENAILNSDFQKQMKNGLYATFEHNDHKVTYSLVEAKGPNKTSLTPKDTSADYKTDSNEIVYPDVFPNIDLQTFTFNENIKEDLVLHQYDGYNTFTFQVKTDLQAKEQEDGSIDFSDEKGKVVFSVPKPFMTDSKLDELSGEVERSDKVSYKLEKNEEGYLLHLTADENWLKDPERVYPVSIDPSTSLSVSSDTFVMSAYPTTNYSASSQKWDANLKSYVLKTGYYDKTTGTNYAFMKFNNLKPIQNMAVTKATLKTYVAHSYYGTKATGLWLDTVNSNYDNGKVTWNTKPASKNIGKAEVHKGQWASYDVTAAVKSWNSGGANYGFKLHTNGNGKEYWKKLISSANSANKPYIEVTYTIPKGNTPSIKAYHNGDSTGYFDISWKKVEGAKGYKVWIYNGKEYQAISAGNVTSWSTKGKKIWPTSAEIASKRYKLHVDGKDGAELALDPSPVYKNSGGSYATSKNYWIGVSAIFDQGEGAMSAPAKPVIPNVGKAQAPSTKGYNNGNATGYFDLSWKAVSGATGYKVQVFNGKGFETLDLGNQTSWTTKGKKIWPTSAEIKAGKYALHLKDGNGAELPINPGPTYKNAGGDGAKKNYSFKIIAYNKDGEAIASPAANPTLPDIAKPKNLTGYLYTNTKSSQTGYVNLIWEKVQNAKGYKVNIYNGKEYQSYDVGDVDHWTTQNKNIWPTPEEIKAGSYKLHTDGKGRELALDPSPVYNNANGNYKGKKNYSFTLSAYNANGETIPTAPFNPTFHEGAEFLGTEEYWSIIDIPSGQLNGATGNVIVNEEDLSIDGRGPGLGLSRTYNSLDTSDHLFGQGWYADAETSVISTDGGAMYIDEDATTHRFTKKADGTYQPPTGVYLELTETADQFILKTKDQTNAYFNKKGGKLQKVVDGHNNATVYTYNDKNQLTAITDASGRKLTFTYDENGHVTSITGPKNKKVTYSYESDLLKKVTDTDGTVTSFDYDAEGRLVKQYSANSTEAKPVFTEYQYSGHRLEKAINAKKETYVYSYDADKKTLLMTQPNGRKVQYGYNEAGNPIQVIDDAEGLKITTNTKYEGNNVVEDVDPNDVGTGKATESYQYDKDGNVTSVKDAYGTETYEYNKNNDVTKMKDTEGNVTDIAYDGLDAVSETDQSGKSSSAAVYDKYGNQIQSSKDLSASTNILKDGSFEAQKSGWNLTASKDSGKISVIADKSGVLSGSKALEVLSQSTSAGTDHGYSSATQTVELEPNTTYTLSGKIKTDLAKSRAYFNIDLRDKDQKRIQWIHNEYSALAGKNDWTKRQITFTTPANAGKAVVYMEVDHRDKDGKGKAWFGEVQLEKGEVSSSYNPVQNSSFTAATENWSVSGASVDSEEGFNDDVSLKAARTSASQAGSVTKQTVVLGQNANDKPVYLTLTGMSKASSVKFTDEKDYSLQANVTYADGSTGVYNAKFPSGTQEWNRAAVVIPKTKPINKVDISILFQKSATGTVWFDDIRLIEGSLLTKSTYDSNGNYVTKEEDELGFSTSTDYDETGKKTAETDAKGEKTTYTYDQADQLTNMTLSNGTSILHSYDKEGNEVSKTIRAGADQTYKYEYDVMGKLVKTTDPLGNVLASEYDANSNLTKTISPNGNEVSLSYDGTDRVKSKSYNGTEKYNFTYDKNGNETSVVNKEQNTTKKRTFDNKNRLTELTDRGGSQTWTYPSDSDKLKTFSWSHGDQKGTNQFTYNKLDQMIEMKDSTSSYSFDYDENGNVQTFITGNGGGTSFSYDERNLVSSLHIGDKNGGSILTESYEYDANGNRTTINSSASGKVQYEYGKLNQLVKETHEDGTVIEYTYDGFGNRKTVTTVKDGSSKTVNASFNIMNQLTKVNDESISYDKNGNRTSDGKFTYTWDAEDNLTAVTKKGEDKPFATYKYDEKGNRIQKTVNGNVTNYFYDGDSLNVLYETDADNKVTKSYTYGDSGQLLSYTENGKKYFYHYNAHGDVIAISDSSGKTLAKYQYDAWGNPTKTEASDEVKDNRYRYAGYQYDEETGLYYLMARYYEPRNGVFLSLDPDPGSDGDSLDQNGYAYGNNNPVMNVDPDGHWVWLVVNAGFAAYDGYKAYKSGKGWKGAAWAAASNFGPGKIFKGAKRVYRFAKSGKNFNWKHIKKDHGPKSKARMPNGQPKSKFRSAKTLRRTTKATARTRPAYVQKDGRTVHFKKFKKPIGRKTNGRHTYTVKVVKSGRYVVTSYPY