Protein: HC-toxin synthetase (HTS) (EC 6.3.2.-) (Cyclic peptide synthetase HTS1) (Non-ribosomal peptide synthetase HTS1) (TOX2 HC-toxin biosynthesis cluster protein HST1) (Q01886)
Organism: Cochliobolus carbonum (Maize leaf spot fungus) (Bipolaris zeicola)
Function: Non-ribosomal peptide synthetase, able to activate proline and AEO (2-amino-9,10-epoxi-8-oxodecanoic acid), and epimerize L-Pro. Catalyzes the production of HC-toxin: a cyclic tetrapeptide. Activates and thioesterifies L-Pro, and epimerizes it to D-Pro; also uses D-Ala as a substrate but this is epimerized from L-Ala by TOXG.
Interaction partners:
Sequence:
MTMPHHSSGPAKDSPLCRFPPFPGGNPVFTNIRREKVNFQLPPPDHALLAAAWAVLLRLYTGHVKTCFESATSDQEANLVTYEARDSDTLQTIVLRGACVSSTAEEKAGLRDLNTAVVRTTVSIDSWTDEMLQDKIAALLQPGKEIVLFQTPSGCVLVYMQSFMSAMEVKNVSSTLTYIMSSDPDKTAIRNLSISPRDLAQIMRWNDRKLKSERTNLVYDLFSARAHEQDANMAIDAWDGRMSYTELERVSSTWARQLQKQGISQGSWVLFCFEKSRLAVVSMIAILKAGGVCVPIDPRYPVERIRDIIRTTNATIALVGAGKTAALFKSADTAVQTIDITKDIPHGLSDTVVQSNTKIDDPAFGLFTSGSTGVPKCIVVTHSQICTAVQAYKDRFGVTSETRVLQFSSYTFDISIADTFTALFYGGTLCIPSEEDRMSNLQDYMVSVRPNWAVLTPTVSRFLDPGVVKDFISTLIFTGEASREADTVPWIEAGVNLYNVYGPAENTLITTATRIRKGKSSNIGYGVNTRTWVTDVSGACLVPVGSIGELLIESGHLADKYLNRPDRTEAAFLSDLPWIPNYEGDSVRRGRRFYRTGDLVRYCDDGSLICVGRSDTQIKLAGQRVELGDVEAHLQSDPTTSQAAVVFPRSGPLEARLIALLVTGNKDGTPHNQQSLPKPAFAQCPPDLVKYATSSLQQRLPSYMVPSVWLGIDFLPMSVSGKLDRAVLQDQLESLSPSDYAEILGTTGLEVDPGGAASSVASDSDLRDMNDDSLLLTACSRVLNLPAGKISYSQSFIHAGGDSITAMQVSSWMKRFTGKRIGVKDLLVSPSISTAASCIKSAQDGSRNFVAVRPGQRIPVSPIQKLFFQTAEASKSWNHYHQSFLFRIDQPIKPQTIEDAISLVMQRHPMLQARFERTEEGDWYQYIPIDVERRASVEVIGSLSTDDREAAMLRARQSIDLTEGPLIRCQLFNNNVDEASRLFFVVIHHAVVDLVSWRIIMEELEAHLATDSTPDRGEAYQESVPFLAWCQVQAEAVKDIPVDRTVPLIPKIPTADFGYWGLKHDENVYGNTVERKIPLGHSITEDLLYKCHDSLHTKTIDVLLAAVLVSFRRSFLDRPVPAVFNEGHGREPGGEDAVDLSRTVGWFTTISPVYVPEVSPGDILDVVRRVKDYRWATPNNGFDYFSTKYLTQSGIKLFEDHLPAEILFNYEGRYQAMESEQTVLKPESWHAGEASKDQDPGLRRFCLFEISTAVLPDGQLHLTCSWNKNMRHQGRIRLWLDTLLPAAIGEIVSSLALASPQLTLSDVELLRLYDYSSLDILKKSILSIPAVQTLDDLEGVYPGSPMQDALFLSQSKSQDGAYEVDFTWRVATSLQNSQPAVDIGCLVEAWKDTVALHAALRTVILESSLPATGILHQVVLRSHDPDIVILDVRDVTAAITILDSYPPPTEEGIALIKRPPHRLLICTTIEGSVLIKFQVNHLVFDGMSTDKIIQDLSKAYTCRHSNKLPDHSESKLHDGTYGNRPTKPPLAEFIRYIRDPQRKQDSINYWKNALRGATTCSFPPLFDQITSEKAMPRQSWASVPIPLCVDSKELSKTLANLGITMSTMFQTVWAIVLRIYSQNGQSVFGYLTSGRDAPVDGIDSAVGNFIAMLVCFFDFDDDGVHTVADMARKIHNASANSISHQACSLAEIQDALGLSTSTPLFNTAFTYLPKRPTNVKAGEPEHHLCFEELSMSDPTEFDLTLFVEPTQESNEVSAHLDFKLSYISQAYATSIASTVAHILSELVHDPYRALNTLPIVSEHDTAIIRSWNDHLFPPATECIHETFSRKVVEHPQREAICSWDGSLTYAELSDLSQRLSIHLVSLGIKVGTKIPICFEKSMWTIVTILAVVQAGGVFVLLEPGHPESRLSGIIKQVQAELLLCSPATSRMGALQNISTQMGTEFKIVELEPEFIRSLPLPPKPNHQPMVGLNDDLYVVFTSGSTGVPKGAVATHQAYATGIYEHAVACGMTSLGAPPRSLQFASYSFDASIGDIFTTLAVGGCLCIPREEDRNPAGITTFINRYGVTWAGITPSLALHLDPDAVPTLKALCVAGEPLSMSVVTVWSKRLNLINMYGPTEATVACIANQVTCTTTTVSDIGRGYRATTWVVQPDNHNSLVPIGAVGELIIEGSILCRGYLNDPERTAEVFIRSPSWLHDLRPNSTLYKTGDLVRYSADGKIIFIGRKDTQVKMNGQRFELGEVEHALQLQLDPSDGPIIVDLLKRTQSGEPDLLIAFLFVGRANTGTGNSDEIFIATSTSSLSEFSTVIKKLQDAQRAMEVLPLFMVPQAYIPIEGGIPLTAAGKIDRRMLRKLCEPFNRNDLISFTSKALSTSVKDAETTDTVEDRLARIWEKVLGVKGVGRESDFFSSGGNSMAAIALRAEAQRSGFTLFVADIFTNPRLADMAKLFSHGQSVSPSSSTLRTKVPISSLQKRSSGLQTAAPVSNGSPVRRCQKENIIDCPVAFEYEEGPSDTQLKEASRICGISSRSIEDVFPCTPMQEALVALSLIPGAQASYALHAAFELRPGLDRNRFRSAWESTVKAQPILRSRIISGSNGSSVVVTSATDSIPQLDVSGLDTFLEQQLQVGFAPGAPLFRLAFVYSKADDCDYFVISAHHAIYDGWSLNLIWSQVLALYTNGELPPPGPSFKHFARNLNLVQSKLDSEDFWRKLLVKPDQESFRFPDVPVGHKPATRCTTNFHFPFSMQSKIGTTANTCINAAWAITLAQYSSNKTVNFGVTLWGRDFPMIDIEHMTGPTIVTVPRQVNVIPESSVAEFLQDLQKSLAVVLPHQHLGLHRIQALGPIARQACDFSTLLVVNHGSSISWSELEAADIVPVPLRSSDLYAYPMVVEVENASSDTLDIRVHSDPDCIEVQLLERLMEQFGHNLQTLCRAASFDPGKRIAELMDDTATTHLRTLFSWNSRVKDSPDVAAIAVHKLLEETAQSQPAESAIVAHDGQLSYMQMDRCADVLARQIRKTNMISAQSPFVCIHLLRSATAVVSMLAVLKAGGAFMPVDISQPRSRLQNLIEESGAKLVLTLPESANALATLSGLTKVIPVSLSELVQQITDNTTKKDEYCKSGDTDPSSPAYLLYTSGTSGKPKGVVMEHRAWSLGFTCHAEYMGFNSCTRILQFSSLMFDLSILEIWAVLYAGGCLFIPSDKERVNNLQDFTRINDINTVFLTPSIGKLLNPKDLPNISFAGFIGEPMTRSLIDAWTLPGRRLVNSYGPTEACVLVTAREISPTAPHDKPSSNIGHALGANIWVVEPQRTALVPIGAVGELCIEAPSLARCYLANPERTEYSFPSTVLDNWQTKKGTRVYRTGDLVRYASDGTLDFLGRKDGQIKLRGQRIELGEIEHHIRRLMSDDPRFHEASVQLYNPATDPDRDATVDVQMREPYLAGLLVLDLVFTDEVMGIPCTSLTSANTSENLQTLVTELKKSLRGVLPHYMVPLHFVAVSRLPTGSSGKLDHAFVRACLRELTAPLDGNFPKVEQVLTTNESVLRQWWGTVLAMDPHSIQRGDDFFSLGGSSISAMRLVGLARSSGHKLQHEDIFMCPRLADMAGQISFVQEASVSPTTSPTIKFDLLDDCEVDEVIDHILPQLDMNKELIEDVYPCTPLQESLMAATARHGEAYTMIQSITVLASQLAQLKKAMDVVFRDFEVLRTRIALGPSQQALQVVVKHEELSWESFPSIQSFKDHFYRSLGYGKPLARLAVITQALDTKQPISHGTREARTKNSQDTVMVVVGAHHSIYDAHVLSMIWRRLYREFIGSQADGILEAETSRSEGVVPFKSYVEKLLRGKDNDESLLFWKEKLRGVSSSQFPPASWPRVLEHQPSATQTLITKVSLPTSSRKKLGATVATVAYAAWALTIAHYTADPDVVFGATLSGRETMAGSISHPESIAGPTIITVPLRIIIDFQTVVSDFLSTLQKDIVRAAYFGQMMGLNSIAHIDNDCRDACGFKSIIVVQVPDEGENHDGRAANPFQMSLESIGHFPAPLVVEVEQSESTDVLIRMAYDPVLVPEKLAHFISDTFTTTMSNLSAANPKAKVESIPALSEAHLAELDVTCPEWILGKAKDEKIRTESHQCLQDLVCRRAQQSPNSQAIDSWDGSISYHELDGLSSILAEHLSQLGVRPEAPVCLLFEKSKWAVVAMIGIIKAGGCFVPLDPSYPHERLEHIISETGSSVIVTSAAYSKLCLSLSVRGIVCDGSVFSSTKKPLPSTADSPPSFSVRPNQAAYILFTSGSTGKPKGVVMEHHSVCSALIALGKRMGLGPQSRVLQFNSYWFDVMLLDIFGTLVYGGCLCIPKEEQRMSNLSGWVQKFKVNTMLLSTSVSRLMQPADTPSLETLCLTGEAVLQSDVDRWAPKLHLIAGYGPTETCIMSVSGELTPSSPANLIGKPVSCQAWVINPLKETELAPYGATGELYIQGPTVARGYLHDDVLTSKAFIVDPQWLTGYKTNENQWSRRAYKTGDLVFWGPQSNLYYVRRKDSSQVKIRGQRVELAEIEEVIRQHIPPDVTVCVDLLSSDDQNTRIILGAVLGIGDRALGGPEDLEVIGYMDDLKSHIIPALEASLPHHMIPEAYVPFVQLPTLGSGKLDRKTVRRVAGPLAFSLPQASARHPNQPTVTHTQKLLRQLWCKILPQLDESAVNKQDNFLGIGGDSIAAIKLVALLRQHGISLAVAEIFTRPTLEAMSSLIDEHNFVVSHAGILSDVTRNTSGVMRQTTNLIAGRHSMAVEKSRECDNSTLPCTEYQQMFLAGTEAFTGAHSAQFIFRLPEKIDLDRLQAAFDHCADWYPNLRTQIHKDADTGRLLHDISPIGVKVPWSCHYSDDLNTVLSHDKKFPPGLDGPLHRVTIMRHRDPTESMLVWTLNHAAYDAWSLRMMLEHITEAYANPDYEPSYSLGWTAFVLHTENTKEASRSFWSSYLSDVKPARLMFNYNLVSNPRQDRLYEARINIPKRVLSQATAATVLLAGLTLLVARVCDTRDVILAHLLTGRTLPLAGIENCPGPTITKVPLRIPLMDQDLVTLELDSVAKKITAELMRVMPHEHSGLSAIREFIPQAEGTTTSSGKFHAGSVLGRLPLDLVIHPKGGLDLLGKHGLGLQNEGFRLVAPPSGGLSMECALVDDDDDKRSDTISVDVSVLWDQRAATQEDVIELVHSLQGIFTKRNLAASICLMYK