Protein: SLIT-ROBO Rho GTPase-activating protein 2 (srGAP2) (D4A208)
Organism: Rattus norvegicus (Rat)
Function: Postsynaptic RAC1 GTPase activating protein (GAP) that plays a key role in neuronal morphogenesis and migration mainly during development of the cerebral cortex. Regulates excitatory and inhibitory synapse maturation and density in cortical pyramidal neurons. SRGAP2/SRGAP2A limits excitatory and inhibitory synapse density through its RAC1-specific GTPase activating activity, while it promotes maturation of both excitatory and inhibitory synapses through its ability to bind to the postsynaptic scaffolding protein HOMER1 at excitatory synapses, and the postsynaptic protein GPHN at inhibitory synapses. Mechanistically, acts by binding and deforming membranes, thereby regulating actin dynamics to regulate cell migration and differentiation. Promotes cell repulsion and contact inhibition of locomotion: localizes to protrusions with curved edges and controls the duration of RAC1 activity in contact protrusions. In non-neuronal cells, may also play a role in cell migration by regulating the formation of lamellipodia and filopodia.
Subcellular location: Cell membrane {ECO:0000250|UniProtKB:O75044}. Cell projection, dendritic spine {ECO:0000250|UniProtKB:O75044}. Postsynaptic density {ECO:0000250|UniProtKB:Q91Z67}. Postsynaptic cell membrane {ECO:0000250|UniProtKB:Q91Z67}. Cell projection, lamellipodium {ECO:0000250|UniProtKB:O75044}. Cytoplasmic vesicle, phagosome {ECO:0000250|UniProtKB:Q91Z67}. Nucleus {ECO:0000269|PubMed:17710530, ECO:0000269|PubMed:27748913}. Cytoplasm, cytosol {ECO:0000269|PubMed:17710530, ECO:0000269|PubMed:27748913}. Note=Recruited to actin-rich phagosomes during phagocytosis (By similarity). Translocates from nucleus to cytoplasm during development (PubMed:17710530). {ECO:0000250|UniProtKB:Q91Z67, ECO:0000269|PubMed:17710530}.
Interaction partners:
- Roundabout guidance receptor 2
- Cell division control protein 42 homolog
- Roundabout homolog 1
- SLIT-ROBO Rho GTPase-activating protein 1
- SLIT-ROBO Rho GTPase-activating protein 3
- Actin nucleation-promoting factor WASL
- Formin-like 1
- Intersectin 2
- Intersectin-1
Sequence:
MTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPRFVQVSEDSGRLFKKSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDSTANVRIEEKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDIIDQCCDLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMGDMASQLCAQQPVQSELVQRRQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSMESVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGESQRTDCSLARRSSTVRKQDSSQAIPLVVESCIRFISRHGLRHEGVFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLEHPLFPKDIFHDLIACVTMDNLQERAVHIRKVLLVLPKPTLIIMRYLFAFLNHLSQFSEENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPNPRELEGPIYSRGGSMEDYCDSTHGETISAEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRHNGIDGLIPHQYIVVQDTEDGVVERSSPKSEIEVMSEPPEEKVTARTGASCPSGGHVADIYLANINKQRKRPESGSIRKAFRSDSHGLGSSLTDSSSPGVGASCRPSSQPIMSQNLPKEGPDKCSISGHGSLNSISRHSSLKNRMDSPQIRKTATAGRSKSFNNHRPMDPEVIAQDIEATMNSALNELQELERQSSAKHTPDVVLDTLEPLKTSPVVAPTSEPSSPLHTQLLKDPEPAFQRSASTAGDIACAFRPVKSVKMAAPVKPPATRPKPTVFPKTNATSPGVNSSASPQSTDKSCTV