Protein: YTH domain-containing family protein 2 (E7F1H9)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing (By similarity). Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs (By similarity). The YTHDF paralogs (ythdf1, ythdf2 and ythdf3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation (By similarity). Plays a key role in maternal-to-zygotic transition during early embryonic development, the process during which maternally inherited mRNAs are degraded: acts by binding m6A-containing maternal mRNAs and promoting their degradation. More than one-third of maternal mRNAs can be modified by m6A. Binding to m6A-containing mRNAs results in mRNA degradation (By similarity). Also involved in hematopoietic stem cells specification by binding to m6A-containing mRNAs, such as notch1a, and promote their degradation. The decreased Notch signaling following notch1a degradation promotes endothelial to hematopoietic transition. Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues: polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation (By similarity). The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments, such as P-bodies, stress granules or neuronal RNA granules (By similarity).
Subcellular location: Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9Y5A9}. Cytoplasm, P-body {ECO:0000250|UniProtKB:Q9Y5A9}. Cytoplasm, Stress granule {ECO:0000250|UniProtKB:Q9Y5A9}. Nucleus {ECO:0000250|UniProtKB:Q9Y5A9}. Note=Localizes to the cytosol and relocates to the nucleus following heat shock stress. Can partition into different structures: into P-bodies in unstressed cells, and into stress granules during stress. {ECO:0000250|UniProtKB:Q9Y5A9}.
Interaction partners:
- RNA demethylase ALKBH5
- N
- Pre-mRNA-splicing regulator WTAP
- FTO alpha-ketoglutarate-dependent dioxygenase.
- 2-iminobutanoate/2-iminopropanoate deaminase
- N
- Ras homolog family member Ca
- RNA-binding protein 15
- CCR4-NOT transcription complex subunit 1
- Heterogeneous nuclear ribonucleoprotein C
Sequence:
MSASSLLEQRPKGQANKVQNGAVTQKDTLNDDEFEPYLNAQPRQSNAYTAMSDSYMPSYYSPSIGFTYSLNEAAWSTGGDPPMPYLASYGQLSNGEHHFLPDAMFGQSGALGNNPFLGQHGFNFFPSGIDFPAWGNSSSQGQSTQSSGYSSSYAYAPSTLGGAMIDGQSPFAANEPLNKAVGMNSLDQGMAGLKIGAGDMAPKVVGSGLPGGPLSQVSTAPTMPPASMAPAKTASWADIASKPAKPQPKLKTKGGLGGTNLPPPPIKHNMDIGTWDNKGNMPKPAAPQQTSLPTNGQPPNQSSPQPGATAGGVPQLPLSNGQLVPPTGQLVQHPLPPGGQPGAVPPQLSQGPPASQPSQPTRWVPPRNRANGFGDAAGGPGQSPPNSGMGGITVPAEPHPVLEKLRMVNNYNPKDFDWNPKHGRVFIIKSYSEDDIHRSIKYNIWCSTEHGNKRLDAAYRSLANKGPLYLLFSVNGSGHFCGVAEMRSPVDYNTCAGVWSQDKWKGRFDVRWIFVKDVPNSQLRHIRLENNENKPVTNSRDTQEVPLDKARQVLKIIASYKHTTSIFDDFSHYEKRQEEEESVKKVEVQGSDPYSNNSSRSHYRMQDRQGRVK