Protein: Sterol regulatory element-binding protein 2 (SREBP-2) (Sterol regulatory element-binding transcription factor 2) [Cleaved into: Processed sterol regulatory element-binding protein 2 (Transcription factor SREBF2)] (Q3U1N2)
Organism: Mus musculus (Mouse)
Function: Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 2), which is embedded in the endoplasmic reticulum membrane (By similarity). Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q12772}; Multi-pass membrane protein {ECO:0000255}. Golgi apparatus membrane {ECO:0000303|PubMed:28849786}; Multi-pass membrane protein {ECO:0000255}. Cytoplasmic vesicle, COPII-coated vesicle membrane {ECO:0000303|PubMed:28849786}; Multi-pass membrane protein {ECO:0000255}. Note=At high sterol concentrations, the SCAP-SREBP is retained in the endoplasmic reticulum (By similarity). Low sterol concentrations promote recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi, where it is processed (By similarity). {ECO:0000250|UniProtKB:Q12772}.; SUBCELLULAR LOCATION: [Processed sterol regulatory element-binding protein 2]: Nucleus {ECO:0000269|PubMed:10397761, ECO:0000269|PubMed:11283257, ECO:0000269|PubMed:29068315}. Note=Transported into the nucleus with the help of importin-beta (PubMed:10397761, PubMed:11283257). Dimerization of the bHLH domain is a prerequisite for importin beta-dependent nuclear import (PubMed:10397761, PubMed:11283257). {ECO:0000269|PubMed:10397761, ECO:0000269|PubMed:11283257}.
Interaction partners:
- Sterol regulatory element-binding protein cleavage-activating protein
- Insulin-induced gene 1 protein
- Importin subunit beta-1
- Cellular tumor antigen p53
- Insulin-induced gene 2 protein
- 3-hydroxy-3-methylglutaryl-coenzyme A reductase
- Sterol regulatory element-binding protein 1
- Cyclic AMP-dependent transcription factor ATF-6 alpha
- NPC1-like intracellular cholesterol transporter 1
- Membrane-bound transcription factor site-2 protease
Sequence:
MDESSELGVLETMETLTELGDELTLGDIDEMLQFVSNQVGEFPDLFSEQLCSSFPGGGSNGGSGNNSSGRGNNGGATDPAVQRSFSQVPLSTFSPSAASPQAPALQVKVSPTPPRATPVLQPRPQPQPQPPAQLQQQTVMITPTFSTAPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSPQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTAPIQTAALQVPTLVGSNGTILTTMPVMMGQEKVPIKQVPGGVKQLDPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDSDVDLKIDDFNQNVLLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFLGLSFNPLTSLLQWGGAHNTDQHPYSGSGRSVLSLESGAGGWFDWMVPTLLLWLVNGVIVLSVFVKLLVHGEPVIRPHSRPSVTFWRHRKQADLDLAKGDFAAAAANLQTCLSVLGRALPTSRLDLACSLSWNVIRYSLQKLRLVRWLLKKVFQRWRATPATAAGFEDEAKSSARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNLAECAEEKILPSTLIEIHLTAAMGLKTRCGGKLGFLASYFLNRAQSLCGPEHSTVPDSLRWLCHPLGQKFFMERSWSIKSAAKESLYCAQRSPADPIAQVHQAFCKNLLERAVESLVKPQAKKKAGDQEEESCEFSSALEYLKLLHSFVDSVGFVTSPFSSSSVLRSALGPDVICRWWTSAVTMAISWLQGDDAAVRSRFTEVERVPKALEVTESPLVKAVFYTCRAMHASLSGKADGQQNSFCHCERASGHLWSSLNVSGTTSDPSLNHVIQLFTCDLLLSLRTALWQKQASASQLLGETYHASGTELAGFQRDLGSLRRLAHSFRPAYRKVFLHEATVRLMAGASPTRTHQLLEHSLRRRPTQNTKHGEVDTWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKVGDRRSCSDCQQMIVKLGGGTAIAAS