Protein: E3 ubiquitin-protein ligase MYCBP2 (EC 2.3.2.33) (Myc-binding protein 2) (Protein Esrom) (F1RD40)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Atypical E3 ubiquitin-protein ligase which specifically mediates ubiquitination of threonine and serine residues on target proteins, instead of ubiquitinating lysine residues (By similarity). Shows esterification activity towards both threonine and serine, with a preference for threonine, and acts via two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates (By similarity). Interacts with the E2 enzymes UBE2D1, UBE2D3, UBE2E1 and UBE2L3 (By similarity). Plays a key role in neural development, probably by mediating ubiquitination of threonine residues on target proteins (By similarity). Involved in different processes such as regulation of neurite outgrowth, synaptic growth, synaptogenesis and axon degeneration (By similarity). Required in the visual system for correct fasciculation, targeting and mapping of retinal axons. Acts as a regulator of pteridine synthesis. May play a role in the regulation of the circadian clock gene expression (By similarity).
Subcellular location: Nucleus {ECO:0000250|UniProtKB:O75592}. Cell projection, axon {ECO:0000250|UniProtKB:Q7TPH6}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q7TPH6}. Note=Localizes to axon shafts and associates with microtubule cytoskeleton. {ECO:0000250|UniProtKB:Q7TPH6}.
Interaction partners:
- F-box/SPRY domain-containing protein 1
- Uncharacterized protein.
- Ubiquitin-ribosomal protein eS31 fusion protein
- Ubiquitin-ribosomal protein eL40 fusion protein
- mRNA export factor
- WDFY family member 4.
- Uncharacterized protein LOC794380
- Tuberin
Sequence:
MMCAAAAAGAGGSGILSSSSHSMGLGVRVIPGAGNDFAPIGSGMGSCPVVGARSDCRSRYQLLLSGRALAERYRRIYTTAINDKEQGLNLGRGKKALSKKKLKRRQKVKSKVKTRTKTDTLDGAFPVPDIKLHSNPSAFNVYCNVRHCVLDWQQKEAALALASRNSVQSGDSDSEEEEEYREPFVKLPKIIGIGLCGVFELIKETRFSHPSLCLRSLQALLDMLQGQQPESFQTEPPDVLESLFHLLLETTVRSTGMNDPTGQTLTALSCACLFSLVVAWGDTGKTLQAVSAILTNNGSHACQTIQVPTILNALQRSVQAVLVGKIQIQEWFGNGIKRAALMNKWVLKEVNIDDDEHCLLQTDGSFLYLLCKDGLYKVGSGYSGTVRGHVYNSTSRIRNRKEKRSWLGFAQGCLLYRDMNSHNMAAIKINPETLEQEGTITVPGLQADGQNIIFTDGEYINQIAACKDDGFVVRIYATSSDPALQQELQLKLARKCLHACGISLFDLEKDLHIISTGFDEEAALIGAGREFALMKTASGKIYYTGKYQSLGIKQGGPSSGKWVELPVTKSPKIVQFSVGHDGSHALLVAEDGSVFFTGSASKGEDGESTKSRRQPKPYKPKKMIKLETKMAVYTACNNGSSSIVTKDGELYMFGKDAIYSDSTCQVSDLKGHFVTQVAMGKAHTCVLTKSGEVWTFGVNNKGQCGRDTGAMSQAGKAFGVENMATAMDEDLEDELDEKEEKSMMCQPGMHKWKLDQCMVCTVCGDCTGYGASCVSSGRPDRVPGGICGCGSGESGCSSCGCCKACARELDGQEARQRGIFDAVKEMIPLDLLLGVNIEEHIQIRQEEKRQRINRRHRLEEGRGPLVFPGPLFMNQREQVLARLRPLQAVKLMREKLKDGSSERGDKDASKITTYPPGAVRFDCELRAVQVSCGFHHSVVLMENGDVYTFGYGQHGQLGHGDVNSRGSPTLVQALPGPSTQVTAGSNHTAVLLMDGQVFTFGSFSKGQLGRPILDMPYWNAKPSPMPNIGAKYGRKATWIGASGDQTFLRIDEALINSHVLATSEIFANKHIIGLVPTSISEPPPFKCLLINKLDGSCRTFNDSEQEDLQGFGLCLDPVYDVIWRFLPVTREMCCYNAVIADARVPTASDIQALCSILSPELALPSGSHASTTRSHGALHILGCLDTLAAMQELKMGVASAEEETQAVMKVYSKEDYSVVNRFESHGGGWGYSAHSVEAIRFCADADILLGGLGLFGGRGEYTAKIKLFELGPDGGDHETDGDLLAETDVLAYDCAAREKYAMMFDEPVLLQLGWWYVAWARVSGPSSDCGSHGQATITTDDGVVFQFKSSKKSNNGTDVNAGQIPQLLYRLPSNDGNASKGKQQTSEPVHILKRTFARTVSVDCFESLLSILHWSWTTLVLGVEELRGLKGFQFTATLLDLERLRFVGTCCLRLLRVYICDIFPISASTKAIVEESSKLAECVGKTRSLLKKILSEGMDNCLTKLDNDPQGYLSQPLTLLEAVLQECHNTFTACFHSFYPTPALQWACLCDLLNCLDQDIQEANFRTSSSRLLAAVMSALCNTSVKLTSILPIAYDGEVLLRSLVKQVSTENDSALAHRFPLLVAHMEKLSHTEENLMGMTTFREVLEKMLVIVVLPVRKSLRKEVELFSPHLVSNTCGLLASIVSELTASALGSEVDGLNSLHSVKATPNRFTKTSQGRSWNTGNGSPDAICFTVDKPGVVLVGFCVYGGGGIHEYELEVLADDAQTEHPGDSAHSHRWTSLELVKGTYCTDDSPSDIAEIRLDKAVPLKENVKYAVRLRNYGSRTANGDGGITTVQCSDGVAFTFSTCSLSSNGTNQTRGQIPQILYYRSEYDGDLQSQLLSKANEEDKNCSRALSVVSVVVRAAKDLLHRAFAVDVEDIPELLSSSSLFSMLLPLILAYIGPVAASVPKAAVEVFGLVQELLPAVSALNQKYAPPTFNPNQSTDSTTGNQPEQGLSACTTSNHYAVLESDHPYKQAGVTQYKVSFPDCVRWMTVEFDPQCGTAQPEDVLRLLIPSRSLHFSGLSSKALAHETINSWTELKKFSGSSGWPTAVLVLPGNEALFSLETASDYVKDEKASFYGFKCVAVGYEFNPGLDEGIIQLEKELAYLGSVCAAALMKKDLALPIGNELEEDLEILEEASLQVCKSHSGLLGKGLALSHSPTILEALEGNLPLHLQTNEHSFLEDFITCVQSSSGGRLARWLQPDSYADPQKTSLILNKDDIRCGWPTTVVVQTKDQYGDVVHVPNMKVEVKAVPVSQKKSIQQENMKKLQRLPGTSSNSATGTDLTFGGHPAPKLEATYEPMIIKEARYIAITMMKAYENYSFEELRFASPTPKRPSENMLIRANTDGTYSANWTPGAVGLYTIHVTIDGIEIDAGLEVEVKDPPKGMIPPGTQMVKPKAEPQPSKVRKFVAKDSAGLRVRSHPSLQSEQIGIVQVNGTITFIDEIHNDDGVWLRLNDETVKKYVPNMNGYTEAWCLSFNQHLGRSLLVPVDVINSEGTWVQLDKNSVVEFCESDEGEAWSLARDRGGNQYLRHVEEQAVLEHGAQTPPPSPFSVQAFNRGMASSGAQGFDYGISNNKGDRDNMASWSVSPGSKHRQESRSSKTDSHSNRSVDQVKSKNNESLSASEALILKSDTGKLRSDSHSRSHSPNHNTLQALKADGRTSGLRAESPNPGSRSSSPKQKTFTSGRSSPSSTSSPRSSSPHDKNLPAKVSPSKVHLDPPRERSKSDSYTLDPDTLRKKKVPLMEPLRGRSTSPKPKLPPKESKGGSSNAENRAPSPHVVQENLHSEVVEVCRSSALLSNDEGNDENSELHNAEEGSSKVHFSIGKAPVKEELESRSSPKVSRKTSSRHVRPKKDKSGPLFKGENVRPTEPAKQAMSPSVAECARAVFAAFLWHEGIVHDAMACSSFLKFNPELTKEHAPIRNSLSCQQGFDEKESKLKNRHSLEISSALNMFNISPHGPDISKMGSINKNKVLSMLKEPPLPEKCEDGKESVSYEMTSHSSMRSKSILPLTLQHLVAFWEDISMATIKAATQNMIFPSPGSSAILKKKENEKDSKKTKKEKKKKEKAEVRPRGNLFGEMAQLAMGGPEKDTICELCGESHPYPVTYHMRQAHPGCGRYAGGQGYNSIGHFCGGWAGNCGDGGIGGSTWYLVCDRCREKYLREKQTAAREKVKQSRKKPLQVKTPRALPTMEAHQVIRANALFLLSLSSAAEPSLLCHHPPKPFHSHLPSLKEGVSEELPNKMGCLYLQTLARQHTENFGVYQDDNLFQDEMRYLRSTSVPAPYISVTPDACPNVFEEPGSNMKSMPPSLETSPITDSDTAKRTVFQRSYSVVASEYDKQHSASPARVKAVPRRRVHSGDAEVGSSLLRHPSPELSRLISAHGSLSKGERNFQWPVLAFVIQHHDLEGLEVAMKHALRKSACRVFAMEAFNWLLCNVTQTTSLHDILWHFVASLTPSPFETEEEEDEENKGNKENLEQEKDLGVCEHPLSDIIIAGEAAHPLPHTFHCLLQTISDLMMSLPSGSSLQQMALRCWSLKFKQSDHQFLHQSNVFHHINNILSKSDDGDSEESFNISVQSGYEAISQELCVVTCLKDLTSVVDIKTSSRPAMIGSLTDGSTETFWESGDEDKNKTKSITISCVKGINASYVSVHVDNSRDIGNKVTSMIFLCGKAVEDLCRIKQIDLDSRHMGWVTSELPGGDHHVIKIELKGPENTLRVRQVKVLGWKEGESIKIAGQISASVAQQKNCEAETLRVFRLITSQVFGKLICGDAEPTPEQEEKNLLSSPEGEDKAPSDADLKEHMVGIIFSRSKLTNLQKQVCAHIVQAIRMEATRVREEWEHAISSKENANSQPSDDDASSDAYCFELLSMVLALSGSNVGRQYLAQQLTLLQDLFSLLHTASPRVQRQVTSLLRRVLPEVTPMRLASVIGVKALPPADISDIIHSTEKGDWTKLGILDMFLGCIAKALTVQLKAKGTTIVGTAGMAAGKGVTTVTLPMIFNSSYIRRGESHWWMKGSTPPQIAEIIIKLVKDMAAGHLSDAWSRVTKNAIAETIIALTKMEEEHRSPVRCIATTRLWLALASLCVLDQDHVDRLSSGRWMGKDGQQKQMPMCDNHDDGETAAIILCNACGNLCTDCDRFLHLHRRTRTHQRQVFKEEEEAIKVDLHEGCGRTKLFWLMALADSKTMKAMVEFREHTGKPASSSSDACRFCGTRHGTELSAVGSVCSDQDCQEYAKLACSKTHPCGHPCGGVKNEDLCLPCLHGCDKTATCLKQDADDMCMICFTEALSAAPAIQLDCSHVFHLQCTRRVLENRWLGPRITFGFMSCPICKNKINHLVLKDLLDPIKELYEDVRRKALMRLEYEGLHKSEAITMSGARFFNNPAGFAMNRYAYYVCFKCKKAYFGGEARCDAEAGQGDDYDPRELICGACSDVSRAQMCSKHGTDFLEYKCRYCCSVAVFFCFGTTHFCNACHDDFQRMTSVPKEELPHCPAGPKGKQLEGSECPLHVVHPPTGEEFALGCGVCRNAHTF