Protein: E3 ubiquitin-protein ligase Ubr3 (EC 2.3.2.27) (E3 ubiquitin-protein transferase Ubr3) (Q9W3M3)
Organism: Drosophila melanogaster (Fruit fly)
Function: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues, leading to their ubiquitination and subsequent degradation. Binds to the E3 ubiquitin-protein ligase Diap1 and enhances its ubiquitination and anti-apoptotic functions. Essential during trichome development for the ubiquitination of the N-terminus of ovo isoform B (svb), converting it from a transcriptional inhibitor to an activator. Positively regulates a hh-signaling pathway which functions in photoreceptor differentiation. Activation of hh up-regulates transcription of Ubr3, which in turn promotes hh signaling by mediating the ubiquitination and degradation of cos. Necessary for auditory transduction: plays a role in Johnston's organ organization by acting in the regulation of zip and ck function in scolopidial apical attachment. Likely to function by acting in a pathway that negatively regulates the ubiquitination of zip, consequently affecting its interaction with ck. May also negatively regulate a component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex Cul1, which also appears to function in the negative regulation of the zip-ck interaction and scolopidial apical attachment.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:26383956, ECO:0000269|PubMed:27195754}. Nucleus {ECO:0000269|PubMed:26383956}. Note=In Johnston's organ, high expression at the apical tips of neurons and scolopale cells (PubMed:27331610). In the cytoplasm expressed in puncta (PubMed:27195754). {ECO:0000269|PubMed:27195754, ECO:0000269|PubMed:27331610}.
Interaction partners:
- Transcriptional regulator ovo
- Peptide tarsal-less AA
- E3 ubiquitin-protein ligase Ufd4
- LD24839p
- Ubiquitin-conjugating enzyme E2-17 kDa
- Death-associated inhibitor of apoptosis 1
Sequence:
MDEDDNLSNADISIDDEEVVREQTHHPPMQEDQELDNEDGSSDVDLSSVYVVPPIVSAPSPASVRIAGGSTIGGGGVAAGAVPTTTDNSHSPFHDWDSSVAAAAAPPPPPPRTGPTTTTSSGTAAESGAASALSDDAGAKPSFFFGASSFSLRSRKEVAALINTECCRGSPTPDLDSIMDTLFNPGTPIDNLDNIEWIRWLIAGGRTPQEFVKIVRSYDNHAKCGLVWVPHVVAYRCRTCGISPCMSICRDCFKKGNHTNHDFNMFLSQAGGACDCGDTSVMKAEGFCSDHGINNRVNRDPVPNNLLAVAEAIMPKLLFRLLQHFREHSDTPLEVQAITSYSCEEFANMLIDLNNMGEIMRKVMTRTLINPEVYAFFMEAPCQDTRNGRFLKANREKYEDAVNRFPNPEPPDEYRDLPALGDKLVHTTLLEEFIFWTFKFEFPQTLVCFLLNMLPDQDYKEHLTRTFVMHYSRIPSVLEMSRDPDTLSNRVVHMSVQLFSNESLALKMVNELSLLHVMIISLKLMMSKILIQNTLHDPNKNFHFVIDCTRQVMKDHCYWPLVSDFNNVLSHESVALVFLRDDNLIDMWFQFLQMLQGMNVNVRETASHVEFEPNSYYAAFSCELEASAYPMWSIISHLQDGTHAHLAKKIINYCVTTLHEWLDSIYFMEARLSMEEMMQASFHFPLHRYLAAFVCQAVTKMGISLNDVLPSRPYLLPLLMIHPLRVQSFFYEILAGKWVRNGLQIKGQAMTYIQANFCNSMADMDLFFLQICATNLPQYFFLQNTIELFDVGQWLETAPLKQPQKAEQSSMLEGFLTFLATLVTSRTNLGNDEATQCIIEISALLATENKTHSQLLELMPERSGNVHTKNFETFLKKLSVYKAPSSGSENLEQGLFTPIDEVWEKHYDPLHVLLRAVHRRDFQSSLDRFTNYVKSKDKMPASGNLWPPFRLPHALPATSSFSDPCKILNSRIFHSTILSIFFRAVHTRDVSEHLLALAVFLLEIAVETSDDVGSGTGDRAPPALAAVVESSSGPGYHGYGTGRHEPPKLFHCYPTDNLSCNLRHVVKKVSLKSRDPQVITSSYRSNPFYSDLDFEVEADPEQSMRMIGQGDPEGDEATGGAGLGMGAHSRRNVSQALVPMRVPGMEVALPPDLSVVAETGVVIRQDSNEDDLLREGNHAMDMSPPAALDFHFPLQQITLPESGMEVAIRRDLLLAETNNMGAIAGGAGGGANASATPPAGASNEMFSPTTPTGSGMLLPFQRVQPVAVPSSGNMDIVPSNAMGAGGSFTSGVSGSGTGRRMNYETGGARKRSVDIAIGGSNKDELHLDESILSLLLKLHSQLSGTLDSFSLSDGEDQSSDDDSTMDVDCNEASTSMAAAESTALAERGRSKRNYKNIHVSSSRIGDGPFFIGNLLRKIAKQDEQCAQSIDDIRARLWPNQREKQAEAKAREAKEKEERRKKARERQQKMMQDFANKQKLFMQSAAASSSGMGYGPEDEDDEELYEEQPREKEYDCIICNCTTPSTESNPIGLVVLVESSGIVGHRRRIAERLPLPINAEDESRLAHTTRLAAEFNRRTELLSLKFGDESWYLSNNMAYDNGVHVQSCGHHVHLSCLEAYLKTLYTTQRQPVQDRGEFYCPVCRQLSNSVLPLSPQLDRPTHLVRSGNQPFERLVADLTDLIKENETIPQPTKLTEAMGHAMEVMTNIAQRKVKCSSITFRKLFIFVTSIARTNLEAEIIQRGGSLCTANATRYKPKRECIVPLLHVLSVHVRVLVEWPLWSSWASLAGLPVTATEPLPAHCLELIPSILADPIALLLKFILLAPLQLDQDYFTCMVKVMYNLLYYQIVVQLCVTLTDLECDHIVKVYGSTSVGSDNSAAESQQQESAAGTTNNRRRAGQQQQSSSQLGKAMALVLSQTNDLVHLRRDCIPSTSSSAAASAAGSSSTTSTNHGASAATASSATTIEVNLKSMELQLQALCLPFLRVAALLRQHLYRHEMPEISAPGLEFVRLVYYLELVTDSMDWDCFNASKGLCFIPGTETTLPQFWCQQLMEVRPPADTVRELVLINQHSLWQQPRLLELPREYERLFTYYHERPCLNCYKVPKESSICLLCGTIVCLKQNCCAENDCCEAVRHTLSCGGGIGIFLVVTSTYIIVIRGRRACLWGSLYLDDFDEEDRDLKRGKPLYLSKDRFNLLESQWLSHKFAHTKHTWVFHRDLL