Protein: Chromodomain-helicase-DNA-binding protein 8 (CHD-8) (EC 3.6.4.12) (ATP-dependent helicase CHD8) (B0R0I6)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/tp53-mediated apoptosis by recruiting histone H1 and preventing p53/tp53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (ctnnb1) activity. Negatively regulates ctnnb1-targeted gene expression by being recruited specifically to the promoter regions of several ctnnb1 responsive genes. May also act as a transcription activator by participating in efficient U6 RNA polymerase III transcription.
Subcellular location: Nucleus {ECO:0000255|HAMAP-Rule:MF_03071}.
Interaction partners:
- Catenin beta-1
- Catenin, beta 2.
- RuvB-like 1
- Activity-dependent neuroprotector homeobox b
- RuvB-like 2
- Dual-specificity tyrosine-
- AT-rich interactive domain 1B
- Family with sequence similarity 124B
- E3 ubiquitin-protein ligase RING2
- Glutamate ionotropic receptor NMDA type subunit 2B.
Sequence:
MADPIMDLFDDTPLFNLDSLPEDAFSQGSSDPVEEALKLALGQVDPPTDPIPDPGVPILSDVVTDPALIPTPVSVPLQNLQTQQLSQIPHEVSVASAPISIQPSLSVASNSSGAATVLLSSSLGVPVSGAQVTPQQQTQQITAVTQQAAGQHAPKIVILKGPQGQTQVLQGVTGATGSPGKVTLARVLTGTPLRPGMAVVSGGTVLNATSPAQGQVKVGTGVQRLVQTANGPMKQVLLTSVPQTQSQVQTQPVQVQIPVQTQLQSPSQPQQLQAQIQAQTQVALQTQAQTQTPTSPAAAGIRPQSVTLSAVPQQVRFVLGSLPGKLVLQGDQLAALAQAKAGQTGAQAKVLTIQLQVQQQPNQQGAKFQLVSGAANAGGSPQVVQISQGQGGQRLAVPLKLLLQPQSNTVSSAGGAVSVVKVINTSAAGSTSGTTTTAASSGVRLAKIQEPVRRVETLCKQEKANRIVAEAIARAKARGERNIPRVLNQDELPAGQTSADLEGAGGATGAKKKGGGGVGGGGGGSKKKSPSAGGAKMVVGGDKKSKAKTPVIPGGGSKSKSKTKLNTITLVGKKRKRNPSSDHSDVDLSPPVSPRTLEEEMSQKRRSNRQVKRKKYTEDLDIKITDDEDELDADVDVTTTPMPAVGHVQPLGAELPPELDGDGLPSMQFFVENPSEEDAAIVDKILSMRVTKKEARQYTNVEEFFVKYKNYSYMHCEWASLEQLERDKRIHQKLKRFKTKQAQMRNLFQEDEEPFNPDYVEVDRILDESHSVDKDNGEPVVYYLVKWCSLPYEDATWELKEDVDEGKVEEFRKIESRQPRLKRTPRPAASAWKKLDESTEYKNGNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIALLSEMFSAGVQSPFMIIAPLSTITNWEREFSNWTDMNAIVYHGSLASRQMIQQYEMYCKDDKGHLIPGAYKFDALITTFEMILSDCPELREISWRCVVIDEAHRLKNRNCKLLDSLKMLEIEHKVLLTGTPLQNTVEELFSLLHFLEPAQFPSEIEFLREFGDLKTEEQVQKLQSILKPMMLRRLKEDVEKNLAPKQETIIEVELTDVQKKYYRAILERNFSFLSMGATQNSNVPNLLNTMMELRKCCNHPYLITGAEEKIVSELREVYDPLAPDFHLQALVRSAGKLVLLDKLLPRLKAGGHKVLIFSQMVRCLDILEDYLIHKRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCVIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMLDKASLKLGLDRAVLQSMSGNKESSIQQFSKKEIEDLLRKGAYAAIMDENDEGSRFCEEDIDQILQRRATTITIESEGKGSTFSKASFVASENRTDIALDDPEFWQKWAKKADIDMDSLNRKNTLVIDTPRVRKQTRQFSSLRGEGGDLSDLDSDDDYPPHNSRQSRASRRSDRHSGGGYGRTDCFRVEKHLLVYGWGRWRDILSHARCKRRLSERDVETICRVILVFCLIHYRGDENIKSFIWELITPPENGREPQALLNHSGLSIPVPRGRKGKRVKAQSSFDVQKVEWIRKYNPDSLLLDDSYRKHLKHQCNKVLLRVRMLYYLKQEVIGEHADSVLSGADARDIDIWLPEMEQQDVPSGWWDAEADRCLLIGVYKHGYEMYTTMRADPCLCFVERCGRPNEQDINAEQQAADPELGEGGDYDKYSEDPEFKPATRHAKEMYEEGDSVNADGEICVEDRSAPMQVEGPSSGSSDLCYWPTSSSLTARLRRLITAYQRSYRREQLKIEAAEKGDRRRRRCEQATKLKEIARQERQQRWTRREECDFYRVVSTFGVERIKKETDAPEGDEHHMDWNRFRSFARLDKKTDESLTRYFKCFMSMCRKVCHIRPGRGDESQDMSQSLAPITEERASRTLYRVTLLCRLRERVLPHPSLEERLSLAPQTSDLPSWWSIPKHDHELLLAAARHGVSRTELSIFSDPLYSFSQSRLDYLQNQQAQAAAQIHAFSQSQDPAGIKEEGLEDESRLLGVEALCPSDSPAMLLSHSDSKVGIQAGWVWKKSKNNGPSERKLGGGGGGASDSDSDSDSGSSSSSRHSGSSDDSGDSDVEREQAALKMCDGDEENSILSLTPSQEGAPPESLTDPLRVDWPKDRILINRIENLCSLVITGHWPSGRRYISDIQLNTVSDEHELGDDLGYSRVARKINSTLSAEALEGQESEFTVKLLKEEGLKLTFSKQALMPNGEGSARKKRKDHELEDAEGVLHAPRRRDLPNWLKENPDYEVEGDMLELLVNRTKRKRRRKRVEKGAALTGSERVKVIDIRTGKKFAGVFGPALQDLREHLEENPDHAVAPEWSETVRHSGFLPEILFHRLLSPHASIPKKSRHYLHTPSLQTDDPLLGGGEGEMLVSDGAYMMDDEDLEDGGHLTSSHHFLTPAYDVKMEPSALDMDGGDSLSQGGYDSSDREAILDDVIMAPKHSDTSSSSED