Protein: Disease resistance protein RPS4 (Probable NAD(+) hydrolase RPS4) (EC 3.2.2.6) (Resistance to Pseudomonas syringae 4) (TIR-NBS-LRR class disease resistance protein) (Q9XGM3)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Disease resistance (R) protein that specifically recognizes the AvrRps4 type III effector avirulence protein from P.syringae. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. Probably acts as a NAD(+) hydrolase (NADase): in response to activation, catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR) and nicotinamide; NAD(+) cleavage triggering a defense system that promotes cell death. The combined presence of both regular and alternative RPS4 transcripts with truncated open reading frames (ORFs) is necessary for function. RPS4 function is regulated at multiple levels, including gene expression, alternative splicing, and protein stability. When over-expressed, confers temperature-conditioned EDS1-dependent auto-immunity. Heterodimerization with RRS1 is required to form a functional complex to recognize AvrRps4 and PopP2. Abscisic acid deficiency enhances nuclear accumulation of RPS4 and its cell death-inducing activity.
Subcellular location: Endomembrane system {ECO:0000269|PubMed:17997306}. Cytoplasm {ECO:0000269|PubMed:22158818}. Nucleus {ECO:0000269|PubMed:17997306, ECO:0000269|PubMed:22158818, ECO:0000269|PubMed:22454454}. Note=Nuclear accumulation is necessary for triggering EDS1-dependent defense (PubMed:17997306). Found in microsomes when interacting with SRFR1 (PubMed:21079790). Accumulates in the cytoplasm and the nucleus, when associated with EDS1 (PubMed:22158818). Accumulates in nucleus at 22 degrees Celsius, but not at 28 degrees Celsius (PubMed:22454454). {ECO:0000269|PubMed:17997306, ECO:0000269|PubMed:21079790, ECO:0000269|PubMed:22158818, ECO:0000269|PubMed:22454454}.
Interaction partners:
- Disease resistance protein RRS1
- Protein EDS1
- Negative regulator of systemic acquired resistance SNI1
- LRR protein
- Lipase-like PAD4
Sequence:
METSSISTVEDKPPQHQVFINFRGADLRRRFVSHLVTALKLNNINVFIDDYEDRGQPLDVLLKRIEESKIVLAIFSGNYTESVWCVRELEKIKDCTDEGTLVAIPIFYKLEPSTVRDLKGKFGDRFRSMAKGDERKKKWKEAFNLIPNIMGIIIDKKSVESEKVNEIVKAVKTALTGIPPEGSHNAVVGALGNSNAGTSSGDKKHETFGNEQRLKDLEEKLDRDKYKGTRIIGVVGMPGIGKTTLLKELYKTWQGKFSRHALIDQIRVKSKHLELDRLPQMLLGELSKLNHPHVDNLKDPYSQLHERKVLVVLDDVSKREQIDALREILDWIKEGKEGSRVVIATSDMSLTNGLVDDTYMVQNLNHRDSLQLFHYHAFIDDQANPQKKDFMKLSEGFVHYARGHPLALKVLGGELNKKSMDHWNSKMKKLAQSPSPNIVSVFQVSYDELTTAQKDAFLDIACFRSQDKDYVESLLASSDLGSAEAMSAVKSLTDKFLINTCDGRVEMHDLLYKFSREVDLKASNQDGSRQRRLWLHQHIIKGGIINVLQNKMKAANVRGIFLDLSEVEDETSLDRDHFINMGNLRYLKFYNSHCPQECKTNNKINIPDKLKLPLKEVRCLHWLKFPLETLPNDFNPINLVDLKLPYSEMEQLWEGDKDTPCLRWVDLNHSSKLCSLSGLSKAEKLQRLNLEGCTTLKAFPHDMKKMKMLAFLNLKGCTSLESLPEMNLISLKTLTLSGCSTFKEFPLISDNIETLYLDGTAISQLPMNMEKLQRLVVLNMKDCKMLEEIPGRVGELKALQELILSDCLNLKIFPEIDISFLNILLLDGTAIEVMPQLPSVQYLCLSRNAKISCLPVGISQLSQLKWLDLKYCTSLTSVPEFPPNLQCLDAHGCSSLKTVSKPLARIMPTEQNHSTFIFTNCENLEQAAKEEITSYAQRKCQLLSYARKRYNGGLVSESLFSTCFPGCEVPSWFCHETVGSELEVKLLPHWHDKKLAGIALCAVVSCLDPQDQVSRLSVTCTFKVKDEDKSWVAYTCPVGSWTRHGGGKDKIELDHVFIGYTSCPHTIKCHEEGNSDECNPTEASLKFTVTGGTSENGKYKVLKCGLSLVYAKDKDKNSALETKYDMLIGKSFQETSEGVDGRVKKTKGKYVMPVEKNFQETTEGVDGRVNKKKKTRMDNGRPKKKQRSGRDDNQTRMQVELQEGNINSVIMHTVKNF