Protein: Transforming acid coiled-coil-containing protein 1 (G5ECG0)
Organism: Caenorhabditis elegans
Function: Involved in microtubule formation, polymerization and assembly, regulating microtubule nucleation and length. Plays a role in pronuclear migration and mitotic and meiotic spindle elongation during early embryogenesis. In complex with zyg-9, functions during the early stages of embryonic development to regulate microtubule assembly throughout the cell cycle. Specifically, the complex is required for the formation and growth of astral microtubules and spindle microtubules during mitotic spindle assembly. At anaphase, the complex is required for mitotic spindle positioning in one-cell stage embryos. The complex acts in a partially redundant manner with the tac-1/zyg-8 complex to regulate microtubule assembly and processes during interphase, mitosis and meiosis in embryos. Plays a role in injury-induced axonal regrowth, regeneration and microtubule stability in PLM neurons and this may be downstream of efa-6.
Subcellular location: Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:12956950, ECO:0000269|PubMed:12956951}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:12956950, ECO:0000269|PubMed:12956951, ECO:0000269|PubMed:12956952, ECO:0000269|PubMed:17666432}. Cytoplasm {ECO:0000269|PubMed:12956950, ECO:0000269|PubMed:17666432}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:12956952}. Cell projection, axon {ECO:0000269|PubMed:26339988}. Perikaryon {ECO:0000269|PubMed:26339988}. Note=Initially enriched at meiotic spindle poles, but then recruited to the sperm centrosome (PubMed:12956950, PubMed:12956951). Localized to centrosomes early in pronuclear migration (PubMed:12956952). Centrosomal localization is dependent on air-1, tbg-1 and zyg-9 and is also controlled during the cell cycle, with high expression during prophase and metaphase, but with expression reducing from anaphase to telophase (PubMed:12956950, PubMed:12956951, PubMed:12956952). Accumulates at the centrosome during interphase, ready for the next mitotic cycle (PubMed:12956951). Cycles between cytoplasm and centrosome during mitosis in a cell cycle-dependent manner (PubMed:12956950, PubMed:17666432). Diffuse localization along the axon and localizes to perinuclear spots in the perikaryon (PubMed:26339988). Following injury co-localizes with efa-6 and ptrn-1 in puncta of the perikaryon (PubMed:26339988). The zyg-9/tac-1 complex localizes to centrosomes throughout the cell cycle and the kinetochore of metaphase and early anaphase chromosomes (PubMed:12956952). {ECO:0000269|PubMed:12956950, ECO:0000269|PubMed:12956951, ECO:0000269|PubMed:12956952, ECO:0000269|PubMed:17666432, ECO:0000269|PubMed:26339988}.
Interaction partners:
- Zygote defective protein 9
- Aurora kinase
- Probable clathrin heavy chain 1
- TPX2 domain-containing protein
- Spindle-defective protein 2
- Spindle-defective protein 5
- Serine/threonine-protein kinase plk-1
- Serine/threonine-protein kinase zyg-8
- Mitotic spindle organiZing protein
- Tubulin gamma chain
Sequence:
MSLNTTFTKEDGTEVVIPFNGSQNGHPENEEPEVEEAAEPSSSVETLCGATRGDIIVMKHTTKALTELIERLLHSDEFEVRRCSNGQIISQGRCNGTTPGNGIGGGGASSEELEKALKDRDAARAEADKLHANYATLFASFNTVREAANDIRGEYEDARDKLKLAAAEVDEWQAKFLAVKDNANSELERASVEYDDLLRSHDENTKGLRLRVKRQEIELSSKNDEIKVLTNRVSELSQICDQLLNDVDVSDGMSVISTDA