Protein: Guanyl-specific ribonuclease pgl-3 (EC 4.6.1.24) (P granule abnormality protein 3) (G5EBV6)
Organism: Caenorhabditis elegans
Function: Guanyl-specific endoribonuclease which cleaves the phosphodiester bond in single-stranded RNA between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleotide, resulting in the formation of a corresponding 2',3'-cyclic phosphate intermediate. P-granule component involved in germline development. Together with the P-granule component pgl-1, is involved in the formation of P-granules. Together with pgl-1, probably recruits other granule components such as pos-1, mex-3 and glh-1, and RNA to P-granules. In vitro, binds mRNA; this interaction is required for the formation of liquid-like droplets that resemble P-granules. Most likely recruits pgl-1 into P-granules during autophagy. Associates with adapters such as sepa-1 and is required for the accumulation and degradation of P-granules by autophagy in somatic cells. This ensures exclusive localization of the P-granules in germ cells. In addition, may act redundantly with pgl-1 to protect germ cells from excessive germline apoptosis during normal oogenesis and development of the two gonadal arms. This may in part be through regulating the localization of sir-2.1 which is involved in germ cell apoptosis. May protect somatic cells from excessive apoptosis during normal development.
Subcellular location: Cytoplasmic granule {ECO:0000269|PubMed:15238518, ECO:0000269|PubMed:19372764, ECO:0000269|PubMed:21402787, ECO:0000269|PubMed:24140420, ECO:0000269|PubMed:26598553, ECO:0000269|PubMed:27594427, ECO:0000269|PubMed:27650246, ECO:0000269|PubMed:28806108, ECO:0000305|PubMed:19167332}. Note=Localizes to P granules in germ cells at all stages of development except in spermatogenesis (PubMed:15238518). Localizes to the germ precursor cells Z2 and Z3 in embryos (PubMed:19372764, PubMed:24140420, PubMed:27650246). Co-localizes with pgl-1 in P-granules, but localization in P-granules is not dependent on an association with pgl-1 (PubMed:15238518, PubMed:21402787). {ECO:0000269|PubMed:15238518, ECO:0000269|PubMed:19372764, ECO:0000269|PubMed:21402787, ECO:0000269|PubMed:24140420, ECO:0000269|PubMed:27650246}.
Interaction partners:
- Protein sepa-1
- ATP-dependent RNA helicase glh-1
- ATP-dependent RNA helicase glh-4
- Guanyl-specific ribonuclease pgl-1
- ATP-dependent RNA helicase glh-2
- Uncharacterized protein
- Argonaute protein wago-1
- P-granule-associated protein deps-1
- Ectopic P granules protein 2
- ATP-dependent RNA helicase glh-3
Sequence:
MEANKRQIVEVDGIKSYFFPHLAHYLASNDELLVNNIAQANKLAAFVLGATDKRPSNEEIAEMILPNDSSAYVLAAGMDVCLILGDDFRPKFDSGAEKLSQLGQAHDLAPIIDDEKKISMLARKTKLKKSNDAKILQVLLKVLGAEEAEEKFVELSELSSALDLDFDVYVLAKLLGFASEELQEEIEIIRDNVTDAFEACKPLLKKLMIEGPKIDSVDPFTQLLLTPQEESIEKAVSHIVARFEEASAVEDDESLVLKSQLGYQLIFLVVRSLADGKRDASRTIQSLMPSSVRAEVFPGLQRSVFKSAVFLASHIIQVFLGSMKSFEDWAFVGLAEDLESTWRRRAIAELLKKFRISVLEQCFSQPIPLLPQSELNNETVIENVNNALQFALWITEFYGSESEKKSLNQLQFLSPKSKNLLVDSFKKFAQGLDSKDHVNRIIESLEKSSSSEPSATAKQTTTSNGPTTVSTAAQVVTVEKMPFSRQTIPCEGTDLANVLNSAKIIGESVTVAAHDVIPEKLNAEKNDNTPSTASPVQFSSDGWDSPTKSVALPPKISTLEEEQEEDTTITKVSPQPQERTGTAWGSGDATPVPLATPVNEYKVSGFGAAPVASGFGQFASSNGTSGRGSYGGGRGGDRGGRGAYGGDRGRGGSGDGSRGYRGGDRGGRGSYGEGSRGYQGGRAGFFGGSRGGS