Protein: DNA polymerase theta (EC 2.7.7.7) (Mutagen-sensitive protein 308) (O18475)
Organism: Drosophila melanogaster (Fruit fly)
Function: Multifunctional protein with both DNA polymerase and ATPase activities. Might have 3' to 5' exonuclease activity. Plays a role in different DNA repair pathways such as DNA strand cross-link repair and microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks. MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions. Utilizes short microhomologies present in partially and fully single-stranded DNA (ssDNA) as primers for DNA synthesis. Prefers poly(dA)/oligo(dT) as a template-primer. The ATPase activity is necessary during interstrand cross-link (ICL) repair and has a critical role in generating templated insertions during MMEJ. Necessary for processing DNA damage induced by oxygen and N-ethylation. In follicle cells, contributes to double-strand break repair at physiological rereplication forks necessary for survival of fertilized eggs.
Subcellular location: Nucleus {ECO:0000269|PubMed:15961355}.
Interaction partners:
- DNA ligase 4
- DNA polymerase zeta catalytic subunit
- DNA repair protein Rad51 homolog
- FI23547p1
- Exonuclease 1
- Flap endonuclease 1
- Uncharacterized protein, isoform C
- LD36501p
- Double-strand break repair protein
- ATP-dependent DNA helicase II subunit 2
Sequence:
MAFSQSFNFGNSTLMALEKGMQADDKENAQPGNGNIQVQSAGNEVNSEIQEINSEFFRDEFSYEVNQAHKPAEQSVVNVSQVQQHMAVVSNQDSEDQSRSSALNDQICTQSSFEGEDAGADAVLDQPNLDENSFLCPAQDEEASEQLKEDILHSHSVLAKQEFYQEISQVTQNLSSMSPNQLRVSPNSSRIREAMPERPAMPLDLNTLRSISAWNLPMSIQAEYKKKGVVDMFDWQVECLSKPRLLFEHCNLVYSAPTSAGKTLVSEILMLKTVLERGKKVLLILPFISVVREKMFYMQDLLTPAGYRVEGFYGGYTPPGGFESLHVAICTIEKANSIVNKLMEQGKLETIGMVVVDEVHLISDKGRGYILELLLAKILYMSRRNGLQIQVITMSATLENVQLLQSWLDAELYITNYRPVALKEMIKVGTVIYDHRLKLVRDVAKQKVLLKGLENDSDDVALLCIETLLEGCSVIVFCPSKDWCENLAVQLATAIHVQIKSETVLGQRLRTNLNPRAIAEVKQQLRDIPTGLDGVMSKAITYACAFHHAGLTTEERDIIEASFKAGALKVLVATSTLSSGVNLPARRVLIRSPLFGGKQMSSLTYRQMIGRAGRMGKDTLGESILICNEINARMGRDLVVSELQPITSCLDMDGSTHLKRALLEVISSGVANTKEDIDFFVNCTLLSAQKAFHAKEKPPDEESDANYINDALDFLVEYEFVRLQRNEERETAVYVATRLGAACLASSMPPTDGLILFAELQKSRRSFVLESELHAVYLVTPYSVCYQLQDIDWLLYVHMWEKLSSPMKKVGELVGVRDAFLYKALRGQTKLDYKQMQIHKRFYIALALEELVNETPINVVVHKYKCHRGMLQSLQQMASTFAGIVTAFCNSLQWSTLALIVSQFKDRLFFGIHRDLIDLMRIPDLSQKRARALFDAGITSLVELAGADPVELEKVLYNSISFDSAKQHDHENADEAAKRNVVRNFYITGKAGMTVSEAAKLLIGEARQFVQHEIGLGTIKWTQTQAGVEIASRAIHDGGEVDLHMSLEEEQPPVKRKLSIEENGTANSQKNPRLETVVDTQRGYKVDKNIANQSKMNPNLKEIDAQNKARRNSTAHMDNLNPISNDPCQNNVNVKTAQPIISNLNDIQKQGSQIEKMKINPATVVCSPQLANEEKPSTSQSARRKLVNEGMAERRRVALMKIQQRTQKENQSKDQPIQASRSNQLSSPVNRTPANRWTQSENPNNEMNNSQLPRRNPRNQSPVPNANRTASRKVSNAEEDLFMADDSFMLNTGLAAALTAAESKIASCTEADVIPSSQPKEPEVIGALTPHASRLKRSDQLRSQRIQSPSPTPQREIEIDLESKNESNGVSSMEISDMSMENPLMKNPLHLNASHIMSCSKVDETASSFSSIDIIDVCGHRNAFQAAIIEINNATRLGFSVGLQAQAGKQKPLIGSNLLINQVAAAENREAAARERVLFQVDDTNFISGVSFCLADNVAYYWNMQIDERAAYQGVPTPLKVQELCNLMARKDLTLVMHDGKEQLKMLRKAIPQLKRISAKLEDAKVANWLLQPDKTVNFLNMCQTFAPECTGLANLCGSGRGYSSYGLDTSSAILPRIRTAIESCVTLHILQGQTENLSRIGNGDLLKFFHDIEMPIQLTLCQMELVGFPAQKQRLQQLYQRMVAVMKKVETKIYEQHGSRFNLGSSQAVAKVLGLHRKAKGRVTTSRQVLEKLNSPISHLILGYRKLSGLLAKSIQPLMECCQADRIHGQSITYTATGRISMTEPNLQNVAKEFSIQVGSDVVHISCRSPFMPTDESRCLLSADFCQLEMRILAHMSQDKALLEVMKSSQDLFIAIAAHWNKIEESEVTQDLRNSTKQVCYGIVYGMGMRSLAESLNCSEQEARMISDQFHQAYKGIRDYTTRVVNFARSKGFVETITGRRRYLENINSDVEHLKNQAERQAVNSTIQGSAADIAKNAILKMEKNIERYREKLALGDNSVDLVMHLHDELIFEVPTGKAKKIAKVLSLTMENCVKLSVPLKVKLRIGRSWGEFKEVSV