Protein: Transcription-associated protein 1 (Q9HFE8)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Essential component of histone acetyltransferase (HAT) complexes, which serves as a target for activators during recruitment of HAT complexes. Essential for vegetative growth. Functions as a component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction and promoter selectivity, interaction with transcription activators, and chromatin modification through histone acetylation and deubiquitination. SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation (By similarity).
Subcellular location: Nucleus {ECO:0000305}.
Interaction partners:
- SAGA complex subunit Sgf29
- SAGA complex subunit Ngg1
- Histone acetyltransferase gcn5
- SAGA complex subunit Hfi1
- SAGA complex subunit Sgf73
- Probable ubiquitin carboxyl-terminal hydrolase 8
- SAGA complex subunit Spt7
- SAGA complex/transcription factor TFIID complex subunit Taf9
- SAGA complex subunit Spt3
- SAGA complex subunit Sus1
Sequence:
MQLALSHNIPIVIVKYLGKRIRVQLEFKKELAQRSKHLMDVSQCEHWHNRLCDVGLDSKQKANTAIEIRDALDDVLVTEKTNFETFIPLLEDTLSLLEKERPVFSSLAATHRLRIALLELLKKSGSYKGFEAFVNRTFAVLLRIVVNDNEEMAVLALKLVVLLFKDHSSLAKGHVQEFLSIVVENYKSMTTVVSEAFPPRSAPNTPSSHPMSAASSASPAEIGMEHAGPKMIPKASSSFKVTAEFPIIVFLLFQTYKDLIPKMLPLLAPLVLQFISLRPPPQAEARRLAESQKEVFIGVVPSLRRNHLYNDLISAQIKSFSFLAYLLRSFGAALKQFESSIPICTLQLFMDCPSELYQTRRELLVATRHVLSTDYLRGFLPYVDQLLDTKILVGSGITSQHSLRPMAFSMLADMLHYVRMELSPQQIYKVILLYFSILMDDFYTSAIQAMATKLILNLVERIVALEDFSTSRSLLFAILLCLLRKLTSLNFEFMKLRDSLQENADLKQIKIEENKHDLPMFENPTGAAQPSGLDKLKDCIFLFKNTLLGIKPVLFGLKQRNIPLANGSIFTAQEWSEKLHLSSTNEVLLFRRLLVESLKGFSYYQTDEKTGVFKSSKNLAYSQLDSSLTTNPSKLLEEKELLEMLATLFLHLDPSVFVEILESEFPNIFECLVDNLALLHIFQFWMSNEVTSVNCTGIVLSFCCDNLAKIGSGQSTRVSVLLRLFKLAFMTVNVFPEKNAEVLRPHISYIISTSLELTTDAVEPLNYVYLMKALFRNISGGKFDSLYKEILPLLQVMLECFNRLIFTVTSTSQKELYAELCLTLPIRLSVLLPHMNFLMKPLIVALKGPPEIASQGLRIFELCLDNLTQEFLDPLLDSIMPDLLICLWNHSRLNQSNNQLHQSAVRILGKMGGRNRQIYLGTFGFDFLQDENIFPSIQFSFQGSSQNFSLEHSKFLMSSCAVLNNQNSDLEEKKQAFQMVKNSYLLLFASAKPDEDFWESIDTMCRAVVDRMDKNLQQVSNGRLCPDKDESYYLQRSIVSNIFKSLVGSMSCVEFVAEARETINRSLEWLIVLDLVNYADSLQIKDQNIFDNLQSIKMLDLTTCINGIFESLCSENENTRSNALSCIDHYLNAHKMLLNTTLDISKLPSFQNLVTVFCQSCHKELWYQKNAGFLGLKAILSYDSHHKLWIQDRLHDILKALFFILKDTPTDYGVLKLTEVRSFIVDITTQFCILQDVLAPKERANNIINAFSPFFLELLHPNDHVRNTVQQAIENISNNSKLSVVDLLLPIKDRLLSPIFGKPLRALPFTIQIGHIDAITYCLHRSPSFLDLTDELYRLFRETIALADAEDEALVTMLKTSQSKDSSSLRKLRATCLHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQKLPKELLQSGLRPILMNLSDHNKLSVNGLEGLSRLLRLLTNYFKVEIGRKLLQHLNVLSDSKVLETASLSLLKTNPRIEIIVSLVNVFRDLPPLSAQFLGDLLSSVVNIEAVLRKYSNSPLRKPLYSFMDLHANDTWMYILNNARNGDLITRFVGALNDPMSEKLRETAGNYWGKLLELISQPVSVENLAPMYAVDIIATVFPYISANVDAGVISAKFIVLSKSLYGMLSSYNEYLFLPIRRCISSITKMLLSSLKKIEKKLEFSLEVFRFKADDDNDFLPEYIDSLCGCLITSTSAAEKKSIFLVCCSIVGDKSVRPFFKAFLLDKVINPLVFKSCGENNFIDKDVVHSVYTHVWRVSIRDFAEVSGATDSFYMGIMCLTTALCKYHSALLNDYRKSVIMSAWNYIKLEDPMVKQAAYATIACFISAYDTPAKIVTPVYVSILKTYQPEVRAFIEFSLASLLSVLTARLSSPSDSTFPLWAKLPRLVISEDVQGISQPLTVYQFICKAPDLFFSCCSHFIVPMVNALPKLVSFSSASTEPRKLALDIVQTFINWQRKQNESENSETTLFSNSHIEAILTFLIKFLSLFPEPVEENPLSKKGLSLFNDLFSFPRWKDCQLNSNFFEKILVDMDFNDNNYRTVANTLFVFGVILKNRGMEYIQREYSHIIALIDKSLRCGKLPVTHSLEQIILLLLQSHPTQAEEEEDTNEADDFKQLLLSVIHDNLAAATNIESAICYLQIVKSSNPEALDGLLLPLNKCFQKVARDHIVACMQSAIQASGKVTLPSASDTVSKLLISFIEIIRVRMASLGDQRRWFLSVVVQLLEKSSSFELCEHILNVTKEWVIVKRDSFLTVKEKTALLLKMRTFEGRFDNKLYIEACDLLSTIYRDPIFAHTELTARLKQAFLLATASKDTKIRMDFMDIFDSSMSRNVYSRLTFILDATSWDTIPSIYWIKQANYILLGAINAKQPVRLTDNSLRFAPVPMTKPILSSLPEVFSKHNGSAIPLGRFTFFKQLDLFLKRNKELTVQKIIFPLAHIQMLSDADANKLWQYIFPLAWKILSSDNRSDLSKSLIYLLTRDYHIKQVNNRPNVISTLVSSFVKCAAKLELPPHLVKYLGKLYGVYHESVSLLEIQLSDKYDMYQNAKVQESRADAVAELYASLNEDDMFYGHWRRNCKYLQTQVALSYEQLGMWGRAQQLYEQAQTKARSEAIPFSESEYNLWEDQWVMCAQKLQQWDVLTELAKHEGSSELLLECAWRISDWSNNRESLEVAIKSLSDVPTPRKLTFQCFMTLQKSVSQPLAIKEFQQVLSEAIQLALIKWHQLPEKVNQSHYSLLHLFQQFVELQEAATIYSHLNAINFQNLPTNVQLIKSALQVWQERLPNVWDDINLWRDLISWRQIVFSMINRVYLPLVPTIQANSSADSSNPPNTSFLFRGYHETAWLINRFAHVARKHKLPSVCLNQLTKIYTLPNIEIQEAFYKLREQVLCYLQNPRDLKTGLEVVTNTNLMYFNSRQKSEFVTLKGKFLEKLNRGEEANQMYAAAVQIDLGLPKAWAEWGRYNDLLFNKSPDNLSAACNAISCYLQAAGTYQSSKARKMLARVLWLLSLNDSAGTIVKAFESYKGDIPVWHWLTFIPQLLNSLSKGDTKCAPVVLKKIAKSYPQALFFTLRTAREDIAQVKRTEMAAWKSNTTDENKRNEDILSIQSNFLSTNQSTNAPATNKEDGLSKKVWEYIDEIMSILKTAYPLLALTMETLVDQIQAKFKCKNDGDAFRLVVALLNDAVQHSIRLGIVTDEMKLPSSTESNLSLFADNILPDYCKQLFKEDFIVNSNGLKSYIFKLRKWRSYFERLLSKVPKKQYLEQYSSFLCEFHHQKFDEIEVPGQYLLHKDNNNSFSCIERFLPEVELIVGHGVCYRRLSIRSNGGTIHPFVIQYPSARNSRREERFMQLTRYLNDALALNCETRRRCLKFYIPAVIPLSSHIRLLEDQPSSITLQKIYEIYSERNNFSRDDPKELFTNELSKHMMELNSQISQESTDAEKLANRKQLFSRRIGMFENIQKLYSPSTILKDYFSSIFTNYSDLWLFRKNFSYQYACFSFITYILSINNRIPAKLVFSRDSGGVWTTEALPSMVSSTPVYHNGEIVPFRFTPNIKEFIGKTCTEGLLGPSIMAIARALSKPDFDLDMYLGIFIRDDLFWWLAQQTKGVPADFSMLNKVNSNTDLIMRRVASLSQVAYGNLPVNQTAIDYLAQASSSKVLAQMDVLWAPWL