Protein: Mitotic chromosome and X-chromosome-associated protein mix-1 (Lethal protein 29) (Structural maintenance of chromosomes protein 2) (Q09591)
Organism: Caenorhabditis elegans
Function: Essential protein required for both chromosome condensation and segregation and X-chromosome dosage compensation depending on its binding partners. Central component of the condensin I complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases. Converts nicked DNA into positive knotted forms in the presence of type II topoisomerases (By similarity). Central component of the condensin II complex, a complex that seems to play a role in prophase chromosome condensation and organization. Both the condensin complex I and II play a role in meiotic and mitotic chromosome segregation. Plays a role in robust cytokinesis upon the presence of chromatin obstructions. Also a member of the condensin I-like dosage compensation complex that associates specifically with hermaphrodite X chromosomes to reduce their gene transcription during interphase.
Subcellular location: Nucleus {ECO:0000269|PubMed:11914278, ECO:0000269|PubMed:15557118, ECO:0000269|PubMed:18198337, ECO:0000269|PubMed:19119011, ECO:0000269|PubMed:9458050}. Chromosome {ECO:0000269|PubMed:11914278, ECO:0000269|PubMed:15557118, ECO:0000269|PubMed:18198337, ECO:0000269|PubMed:19119011, ECO:0000269|PubMed:9458050}. Note=During mitosis and meiosis, localizes to condensed chromosomes in both sexes (PubMed:11914278, PubMed:15557118, PubMed:19119011). During meiotic prophase, surrounds the chromosomes, localizes between homologs during meiotic metaphase I, and localizes between sister chromatids during metaphase II (PubMed:11914278). During mitotic metaphase, localizes at the poleward face and to the core of the condensed chromosomes (PubMed:15557118, PubMed:18198337, PubMed:19119011). Requires hcp-6 for the localization to meiotic chromosomes (PubMed:15557118). After the 40-cell stage when dosage compensation is initiated, specifically localizes to the hermaphrodite X chromosomes in interphase (PubMed:11914278, PubMed:9458050). {ECO:0000269|PubMed:11914278, ECO:0000269|PubMed:15557118, ECO:0000269|PubMed:18198337, ECO:0000269|PubMed:19119011, ECO:0000269|PubMed:9458050}.
Interaction partners:
- Structural maintenance of chromosomes protein 4
- Chromosome condensation protein dpy-27
- Condensin complex subunit dpy-26
- Condensin complex subunit 1
- Condensin complex subunit capg-1
- Uncharacterized protein C30G12.6
- Condensin-2 complex subunit hcp-6
- Condensin-2 complex subunit kle-2
- CAP-G condensin subunit
- Kinetochore protein ndc-80
Sequence:
MHIKSIHLDGFKSYQKHTDILDFSPTFNAITGYNGSGKSNILDSICFIMGINKLDNIRAKSMHELISHGGTKAIVQVRFDNTDKRCSPFGMEHLDEIVVQRIITAQATGKGCATSYTLNGHAATNGKMQDFFRGVGLNVNNPHFLIMQGRITTVLNMKPEEILGMVEEAAGTKMYDQKKKDAEKTMFLKDAKLKEVDRIFQSSIDPRMVKFREDRKNMVEVTRLKKLKENFSRKYEAFQYFQTCEAVKKSAKEIEDAKKGIEDLGEKFNQLDLDLKNKEDEKKKMEESRDDQHEEAALSAAHLSKQSIMLQKETVKNQLVETINKLKKEGEQINKSLSKDREVLDAKRKEHEDSKAANSKDIQSQSDDEALVTKYRNDLESLTRGTIANDKGEHVSIESEIQSCKSTASQMSSGITAAKKRGERLHNQIKHLEGEKATLSARSKSDIGSADNYQKEVDEINKQLQLLGFNIDADTEKREHAAKLHESITKLKDMDTRLLNSYKDGRYALNYQRPPLHIDKFDEKRDVFGYVAHLIKMKPGCEQFAVAADIALGGVLGNVVVSTQDIARILIDGKAFTSRKTMIPVSENARNASSYNTLPDVKLRRAKEIAEKYNDTVTKMIDLIEYPDFISNTILNAVGQILVVDSLDVAREIAYDEVAKTRMITRRGDDVRTNGIMTGGYNDPGNKPALIALEPMYARRPQIEAQQRELDALNRELQLTEASSQKCRDLNNQLATAMRKLAQVKTNINNSEFGIVVRDLKVHSEEYEKNQAEIEATVKTLKDVEDKIKTLESMKNKDKNSQEKRKKELTALLQKAEQTVAQNKNRGEKARREVMLLQATVEEMEKTIKKDEGIWEQKKKECDELEEKLPNAIAALKDAELEQKAAQAKLNDLKNNQRQISTRLGKIAKECDALIREKAKTKSKREEKEKELTSLQQSEASNRKEARSKLKKFEWLSDEEAHFNKKGGLYDFEGYTVSKGKDEIKELTDKIETLERSCCIQNVSNLDTCEAKVLDIKNKRERITEDFNMLKKTIATLDKKKVDELIRAHESVNKDFGQIFNCLLPDAHASLVPPEGKTVCEGLEVKVSFGGVVKDSLHELSGGQRSLVALSLILAMLKFKPAPLYILDEVDAALDLSHTANIGMMIKTHFHHNQFIIVSLKQGMFSNADVLFQTRFADGHSTCTRLNGGDIAVLCQDKVLQAQALELTDAGKAKKDAAAKKGAQKNDKEPPKKKPIVVDDDDFE