Protein: Phosphatidate phosphatase LPIN1 (EC 3.1.3.4) (Fatty liver dystrophy protein) (Lipin-1) (Q91ZP3)
Organism: Mus musculus (Mouse)
Function: Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels. Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression. Is involved in adipocyte differentiation.
Subcellular location: [Isoform 1]: Mitochondrion outer membrane {ECO:0000269|PubMed:21397848}. Cytoplasm {ECO:0000269|PubMed:16049017, ECO:0000269|PubMed:21397848}. Nucleus membrane {ECO:0000269|PubMed:21397848}. Note=Recruited at the mitochondrion outer membrane following phosphatidic acid formation mediated by PLD6. In neuronals cells, isoform 1 is exclusively cytoplasmic (PubMed:21397848). In 3T3-L1 pre-adipocytes, it primarily located in the cytoplasm (PubMed:16049017). {ECO:0000269|PubMed:16049017, ECO:0000269|PubMed:21397848}.; SUBCELLULAR LOCATION: [Isoform 2]: Nucleus {ECO:0000269|PubMed:11138012, ECO:0000269|PubMed:16049017, ECO:0000269|PubMed:17105729, ECO:0000269|PubMed:19753306, ECO:0000269|PubMed:22134922}. Cytoplasm {ECO:0000269|PubMed:19753306}. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q14693}. Note=Nuclear localization requires both CNEP1R1 and CTDNEP1 (PubMed:22134922). In neuronals cells, localized in both the cytoplasm and the nucleus (PubMed:19753306). In 3T3-L1 pre-adipocytes, it is predominantly nuclear (PubMed:16049017). Translocates from the cytosol to the endoplasmic reticulum following acetylation by KAT5 (By similarity). {ECO:0000250|UniProtKB:Q14693, ECO:0000269|PubMed:16049017, ECO:0000269|PubMed:19753306, ECO:0000269|PubMed:22134922}.
Interaction partners:
- Phospholipid phosphatase 2
- Diacylglycerol O-acyltransferase 1
- Phospholipid phosphatase 3
- Diacylglycerol O-acyltransferase 2
- Peroxisome proliferator-activated receptor alpha
- Phosphatidate phosphatase LPIN3
- 2-acylglycerol O-acyltransferase 1
- Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
- Serine/threonine-protein kinase mTOR
- 1-acyl-sn-glycerol-3-phosphate acyltransferase beta
Sequence:
MNYVGQLAGQVFVTVKELYKGLNPATLSGCIDIIVIRQPNGSLQCSPFHVRFGKMGVLRSREKVVDIEINGESVDLHMKLGDNGEAFFVQETDNDQEIIPMYLATSPILSEGAARMESQLKRNSVDRIRCLDPTTAAQGLPPSDTPSTGSLGKKRRKRRRKAQLDNLKRDDNVNSSEDEDMFPIEMSSDEDTAPMDGSRTLPNDVPPFQDDIPKENFPSISTHPQSASYPSSDREWSPSPSSLVDCQRTPPHLAEGVLSSSCPLQSCHFHASESPSGSRPSTPKSDSELVSKSADRLTPKNNLEMLWLWGELPQAAKSSSPHKMKESSPLGSRKTPDKMNFQAIHSESSDTFSDQSPTMARGLLIHQSKAQTEMQFVNEEDLESLGAAAPPSPVAEELKAPYPNTAQSSSKTDSPSRKKDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGDPGGLPKQASDNGARSANQSPQSVGGSGIDSGVESTSDSLRDLPSIAISLCGGLSDHREITKDAFLEQAVSYQQFADNPAIIDDPNLVVKVGNKYYNWTTAAPLLLAMQAFQKPLPKATVESIMRDKMPKKGGRWWFSWRGRNATIKEESKPEQCLTGKGHNTGEQPAQLGLATRIKHESSSSDEEHAAAKPSGSSHLSLLSNVSYKKTLRLTSEQLKSLKLKNGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKVIISDIDGTITRSDTLGHILPTLGKDWTHQGIAKLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTVLPQGPLLLSPSSLFSALHREVIEKKPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVYSYKQVGVSLNRIFTVNPKGELVQEHAKTNISSYVRLCEVVDHVFPLLKRSHSCDFPCSDTFSNFTFWREPLPPFENQDMHSASA