Protein: Chitin synthase 3 (EC 2.4.1.16) (Chitin-UDP acetyl-glucosaminyl transferase 3) (Class-IV chitin synthase 3) (P29465)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer (Probable). Appears to be responsible for synthesis of the majority of the chitin found in the cell wall periphery. It is involved in the synthesis of the chitin ring that forms in the cell wall just before bud emergence. This ring remains at the base of the bud as the bud grows and ultimately forms part of the bud scar marking the division site on the mother cell. Also catalyzes the synthesis of chitin laid down during mating and spore cell-wall synthesis.
Subcellular location: Cell membrane {ECO:0000269|PubMed:12928491, ECO:0000269|PubMed:17519287, ECO:0000269|PubMed:28346351}; Multi-pass membrane protein {ECO:0000255}. Bud neck {ECO:0000269|PubMed:16818716, ECO:0000269|PubMed:28346351, ECO:0000269|PubMed:8909536}. Cytoplasmic vesicle membrane {ECO:0000269|PubMed:12928491, ECO:0000269|PubMed:28346351, ECO:0000269|PubMed:8970154}; Multi-pass membrane protein {ECO:0000255}. Note=Localizes to a ring on the surface of cells about to undergo bud emergence and in the mother-bud neck of small-budded cells (PubMed:8909536). Delocalized throughout the plasma membrane during heat shock (PubMed:12928491). Also localizes to intracellular membrane-bound particles called chitosomes, proposed to act as a reservoir for regulated transport of chitin synthase enzymes to the division septum (PubMed:12928491, PubMed:8970154). {ECO:0000269|PubMed:12928491, ECO:0000269|PubMed:8909536, ECO:0000269|PubMed:8970154}.
Interaction partners:
- Chitin synthase regulator SKT5
- Chitin biosynthesis protein CHS6
- Chitin biosynthesis protein CHS5
- Protein BNI4
- Chitin synthase export chaperone
- Chitin synthase 2
- 1,3-beta-glucan synthase component FKS1
- Chitin synthase 1
- UDP-N-acetylglucosamine pyrophosphorylase
- Protein BCH2
Sequence:
MTGLNGDDPDDYYLNLNQDEESLLRSRHSVGSGAPHRQGSLVRPERSRLNNPDNPHFYYAQKTQEQMNHLDVLPSSTGVNPNATRRSGSLRSKGSVRSKFSGRETDSYLLQDMNTTDKKASVKISDEGVAEDEFDKDGDVDNFEESSTQPINKSIKPLRKETNDTLSFWQMYCYFITFWAPAPILAFCGMPKKERQMAWREKVALISVILYIGAIVAFLTFGFTKTVCSSSKLRLKNNEVSTEFVVINGKAYELDTSSRSGIQDVEVDSDTLYGPWSDAGKDASFLFQNVNGNCHNLITPKSNSSIPHDDDNNLAWYFPCKLKNQDGSSKPNFTVENYAGWNCHTSKEDRDAFYGLKSKADVYFTWDGIKNSSRNLIVYNGDVLDLDLLDWLEKDDVDYPVVFDDLKTSNLQGYDLSLVLSNGHERKIARCLSEIIKVGEVDSKTVGCIASDVVLYVSLVFILSVVIIKFIIACYFRWTVARKQGAYIVDNKTMDKHTNDIEDWSNNIQTKAPLKEVDPHLRPKKYSKKSLGHKRASTFDLLKKHSSKMFQFNESVIDLDTSMSSSLQSSGSYRGMTTMTTQNAWKLSNENKAVHSRNPSTLLPTSSMFWNKATSSPVPGSSLIQSLDSTIIHPDIVQQPPLDFMPYGFPLIHTICFVTCYSEDEEGLRTTLDSLSTTDYPNSHKLLMVVCDGLIKGSGNDKTTPEIALGMMDDFVTPPDEVKPYSYVAVASGSKRHNMAKIYAGFYKYDDSTIPPENQQRVPIITIVKCGTPAEQGAAKPGNRGKRDSQIILMSFLEKITFDERMTQLEFQLLKNIWQITGLMADFYETVLMVDADTKVFPDALTHMVAEMVKDPLIMGLCGETKIANKAQSWVTAIQVFEYYISHHQAKAFESVFGSVTCLPGCFSMYRIKSPKGSDGYWVPVLANPDIVERYSDNVTNTLHKKNLLLLGEDRFLSSLMLKTFPKRKQVFVPKAACKTIAPDKFKVLLSQRRRWINSTVHNLFELVLIRDLCGTFCFSMQFVIGIELIGTMVLPLAICFTIYVIIFAIVSKPTPVITLVLLAIILGLPGLIVVITATRWSYLWWMCVYICALPIWNFVLPSYAYWKFDDFSWGDTRTIAGGNKKAQDENEGEFDHSKIKMRTWREFEREDILNRKEESDSFVA