Protein: Rho-associated protein kinase 1 (EC 2.7.11.1) (Rho-associated, coiled-coil-containing protein kinase 1) (Rho-associated, coiled-coil-containing protein kinase I) (ROCK-I) (p160 ROCK-1) (p160ROCK) (Q8MIT6)
Organism: Bos taurus (Bovine)
Function: Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress (By similarity). Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability (By similarity). Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation (By similarity). Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1 (By similarity). Promotes keratinocyte terminal differentiation (By similarity). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles (By similarity).
Subcellular location: Cytoplasm {ECO:0000269|PubMed:12034773}. Golgi apparatus membrane {ECO:0000250|UniProtKB:P70335}; Peripheral membrane protein {ECO:0000250|UniProtKB:P70335}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000269|PubMed:12034773}. Cell projection, bleb {ECO:0000250|UniProtKB:P70335}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P70335}. Cell membrane {ECO:0000250|UniProtKB:P70335}. Cell projection, lamellipodium {ECO:0000250|UniProtKB:P70335}. Cell projection, ruffle {ECO:0000250|UniProtKB:P70335}. Note=A small proportion is associated with Golgi membranes (By similarity). Associated with the mother centriole and an intercentriolar linker (By similarity). Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers (By similarity). Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). {ECO:0000250|UniProtKB:P70335}.
Interaction partners:
- Transforming protein RhoA
- Rho-associated protein kinase 2
- Rho-related GTP-binding protein RhoE
- Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
- Myosin regulatory light chain 12B
- Catenin beta-1
- Rho-related GTP-binding protein RhoC
- Sodium/hydrogen exchanger 1
- LIM domain kinase 2
- LIM domain kinase 1
Sequence:
NSKSQMDKDYYQLQAVLEAERRDRGHDSEKIGDLQARITSLQEEVKHLKHNLERVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKLKEERDAREKAENRVVQIEKQCSMLDVDLKQSQQKLEHLIENKDRMEDEVKNLTLQLEQESNKRLLLQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLKKIQELQSEKETLATQLDLAETKAESEQLARGLLEEQYFELTQESKKAASRNRQEITDKDHTLSRLEETNSMLTKDIELLRKENEELTDKMRKAEEEYKLKKEEEINILKAAFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKKKKK