Protein: eIF-2-alpha kinase activator gcn1 (Translational activator gcn1) (Q10105)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Acts as a positive activator of the gcn2 protein kinase activity in response to amino acid starvation. Component of the gcn1-gcn20 complex that forms a complex with gcn2 on translating ribosomes; during this process, gcn1 seems to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of gcn2, and hence stimulating gcn2 kinase activity. Participates in the repression of global protein synthesis and in gene-specific mRNA translation activation, such as the transcriptional activator fil1, by promoting the gcn2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/tif211) on 'Ser-52', and hence allowing fil1-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion.
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:P33892}.
Interaction partners:
- Uncharacterized ABC transporter ATP-binding protein C29A3.09c
- eIF-2-alpha kinase GCN2
- Large ribosomal subunit protein eL19A
- Large ribosomal subunit protein eL19B
- RWD domain-containing protein C1393.09c
- GTP-binding protein 1
- Eukaryotic translation initiation factor 2 subunit alpha
- Histidine--tRNA ligase, mitochondrial
- Small ribosomal subunit protein RACK1
- Small ribosomal subunit protein uS15
Sequence:
MSVEEPGIEAHGHKDRMLYAMLLSKDTSLAFLGSKKIMIDILQHICRTQDIDEESAIAALEDIFETLPRNLSRDARKQIEITINHLVSRLPSIVLPFLVRRLTTIAGRLDRFRSTVSVSFDCLNWVNSMIPNLPEKELQYWILELLPLQSSFLSYALRDGKPSVADSAIKSTRRCYRSLFCKKMDSLKKLVSFLLTETENAILPPKSLPLYGVIISTCYYFHQSPNPRNEISQQAELFSKIMAQNVLMAKPALEKYLYHEFCYSLGLLLSVDQLKLYLLPSIEKALLRSPEIIFSGILSSLAHGFADSKVDASSLILSSVLTSFVNGLKSSNAEVRRNCFQTFKDLSANASDNESLSRVASELITSLRTGKVTASDQRVLFVDALSSLSLKHIDASMLLNELLPLFTKAKESDFNSLASLIVKTLKFLLMNGRNPGDKIYDFLSKSLQRPVAHESMFWLTSLATMAWDLPASDDVQIEFINFFLNNLSILTEKALMSVSGATQNGTYLAPIIYLSFGVNKLSVWNSERISHTLELQDILVKLSTPKNNDVFIFSSKITNKLNDDQSKLWYFQGLCDFAKVSDNLLFSNFVERWFQSVIGVFSFASRENSNRALKILKSAILYRPHLRMSICSQLWNYHADFEKSKSVGKFDSAKYDEISSLFQSLILSSMSADTSNFSNQELVDFDKYLVELLFLSFAFKDKFDWIRFCQVSKRDPATLVSERIHSIIEEIELLLSSAIKDSKETAAIASISMIVFVAPEESIPLFVNVFRNQLLHLNISSVSSTDLEIWKTPEGVLWDNVLEKKSSKKLDKNTKDYETKRWEAEVRAKQSAKKPAKLSKDQQALVDAQLDAEAKIRSRVNLIALSLERGLGIIRSLGEAVQLAPALWVEDAIDVLLFHNVLKYSEPFLKNLAYDTFLLTLKASGFSERLGDRSYSSSLASILAHTFSVNSSENIKELTKSILYKLRFAIEQNYFEPQMFACIFPLLYDLTFNITNSDEEDEAELQLLVTEILEFQALYSASLRRMRSKLIKSLLHLLEIAPTQYQENKNSLLSLCEGLHSTYTDEELNLLLSNLFHPESSIRSAVLQALQAFDLSRFEFIKEIFLELYDDNETNASIAHQISTQNGLDATETSFFELQIFFTQDSDYLQQIIGKSLIDLLDEFEELGQFIPKELMRTYRENALPSAPEYDEYGIIKKETIGRDLGRIARESVAVSFFHISKYLSSNLLLPFLEFLLTASEAEAQIPVTDASQKVSSKMLEAGKLAIFQSGAHQVEALMELFEQKLNVDSLPTDANDRLREATVVLFGTVAQHLPSNDPRLAVVMDSLLSVLSTPSESVQLAVAVCLPPLVKKSLGKSKEYYELLSNKLMNSTSLADQKGAAYGLAGLVKGYGIKAFQDFNILDSLSELISNRQNATHRQVALFAVEAFSRILGIYFEPYLPDLLPLLLTSFGDNANEVREATMDAVKQIMSQLSAFGVKLLLPTLLDGLNEYNWRSKKASVEILGLMSYMAPKQLSVFLPTIIPKLSEVLTDSHSQVRNTANKSLLRFGDVISNPEIQTLVPTLLKALSDCTRYTDDALEALLKTSFVHYLDPPSLALVIPILKYGLRERNAGTKRQSAKIFGLMASLTEPENLAVYLESLMPRLREVLIDPVPDTRATAAKALGSLIEKLGEKKFPTLIPELFNVLRSECSEVDRQGAAQGLSEILAGLGLARLEDVLPEILKNTSSPVPHIRESFISLLIYLPATFGSRFQPYLARAIPPILSGLADDSELVQTASLRAAKMIVNNYATKSVDLLLPELEKGLFDNAWRIRLSSVQLVGDLVFKLAGINRKALQEDEEEEGTHSDVSRKALLDIIGQERHDRILSTLYIVRQDIAAVVRTPAIQIWKAIVVNTPRTVREILPTLTSIIVSNLNSSSNDRRTMCVKSLGDLLKKAGFDVLPQLLPVLKQGLESANSGDRIGVCIALEELINSATPEQLEIYSDDFVYAVRRALMDGDLEVRETAAEAFDSLQSILGDRAVDDVLPQLLKLLESENQSEQALSALREIISRRSSTIFPVLIPTLIKKPVSAFNARALSSLAQVAGVTLNKRLPSILNALMESSLASTGDDLVALNGAIDKVNLSVKDQEGLQILMAHFYSFSESEDFRKRLFAAEHMLVFFQNCKLDYYRYVGDWVRHFITLFEDKSQDVVVAAVAAQNTLVSALRKDQLDSLVSIAYHSLRDVGSQGVNLPAFEVAQGVNSILPIFLYGLMHGTMDQREQSALGIADIVLKTEPSKLRPFVTQITGPLIRIIGERFPVEVKCAILYTLNIILSKISTFLRPFLPQLQRTFAKCLGDPSSEVIRSRAATALGTLITLQTRLAPIITELVSGARTPDAGVRKAMLNALFAVVSKSGQNMNEASAEAIEQLLDEISAESSEHMVICAKLYGALFSHLPDAQAKQLLESKVLSLEIQSEFSVLILNAAVKFGSQKIIELKLSDIVCSIISTASLQKEVTIAENGILALGKALLADIPQSFGNAKNLVEALKVNIEAPPSTSQDSRRLALLIIRVVSKENYSLIKPHISILAPAIFGCVRAIVIPVKLAAEAAFLALFQLVEDDSVLNKYIETLEGPRARSFVDYSRRVAVKLAAAERDRINSGSERVKLEEVEDLAEINAVGRDNEVSTNDP