Protein: Rho family-interacting cell polarization regulator 2 (Q80U16)
Organism: Mus musculus (Mouse)
Function: Acts as an inhibitor of the small GTPase RHOA and plays several roles in the regulation of myoblast and hair cell differentiation, lymphocyte T proliferation and neutrophil polarization. Plays a role in fetal mononuclear myoblast differentiation by promoting filopodia and myotube formation. Maintains naive T lymphocytes in a quiescent state and prevents chemokine-induced T lymphocyte responses, such as cell adhesion, polarization and migration (By similarity). Involved also in the regulation of neutrophil polarization, chemotaxis and adhesion. Required for normal development of inner and outer hair cell stereocilia within the cochlea of the inner ear. Plays a role for maintaining the structural organization of the basal domain of stereocilia. Involved in mechanosensory hair cell function. Required for normal hearing.
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:Q9Y4F9}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q9Y4F9}. Cell projection, filopodium {ECO:0000250|UniProtKB:Q9Y4F9}. Cell projection, stereocilium {ECO:0000269|PubMed:27269051, ECO:0000269|PubMed:32631815}. Cell projection, stereocilium membrane {ECO:0000250|UniProtKB:Q7TP54}. Apical cell membrane {ECO:0000250|UniProtKB:Q7TP54}. Note=Localized in the cytoplasm in cells undergoing mitosis (By similarity). Colocalized with F-actin (By similarity). Accumulates at the leading edge of polarized neutrophils in a chemokine-dependent manner (By similarity). Localized with RHOC within the basal domain of hair cell stereocilia, near the taper region (PubMed:27269051). Detected in punctate pattern forming a circumferential ring at the stereocilia base (PubMed:27269051). Localized to the apical stereocilia of inner and outer hair cells (By similarity). Not detected as a membrane-associated protein in stereocilia (PubMed:27269051). {ECO:0000250|UniProtKB:Q9Y4F9, ECO:0000269|PubMed:27269051}.
Interaction partners:
- Taperin
- Glutaredoxin domain-containing cysteine-rich protein 2
- Chloride intracellular channel protein 5
- Glutaredoxin domain-containing cysteine-rich protein 1
- Transforming protein RhoA
- Radixin
- Fascin-2
- Xin actin-binding repeat-containing protein 2
- Unconventional myosin-VIIa
- Plastin-1
Sequence:
MPPGTKRLRALGAFSAGLPTRLPEIMLVGSQSFSPGGPNGIIRSQSFAGFSGLQERRSRCNSFIENASALKKPQAKLKKMHNLGHKNNNTPKEPQPKRVEEVYRALKNGLDEYLEFHQTELDKLTAQLKDMKRNSRLGVLYDLDKQIKTIERYMRRLEFHISKVDELYEAYCIQRRLQDGASKMKQAFATSPASKAARESLSEINRSYKEYTENMCAIEAELESLLGEFSIKMKGLAGFARLCPGDQYEIFMKYGRQRWKLKGKIEVNGKQSWDGAETVFLPLIVGFISIKVTELKGLATHILVGSVTCETKELFAARPQVVAVDINDLGTIKLNLEITWYPFDVEDTTPSSGPGNKTAALQRRMSMYSQGTPETPTFKDQSFFRWLRLSVLSALRDTFFATLHHNHSVGDLPSLSLNPKALLEFYSNLPDDIFESGKAAEEKRPLSLSFSDLQDGDCVFTSSSATSPSSSHSAHPEITITPAELTHSSLSSQNEGTEDSSSASSRNSLGEDHEPKSHPKSDTVEPGKPGVATRSGTESLFLESSVAEALLQESDEASELKPVELDTFEGNITKQLVKRLTSAEGPVTTDKLFFEGSVGSESEAGRSFLDGSLEDAFNGLFLALDPHKKQYKEFQDLNQEVTHLDDVLKCKPAGSRSRSSSLSLTVESALESFDFLNTSDFDEEEEDGDDVCHVGGGADSVFSDTETEKSGYRSVHPEARGHLSEALTEDTGVGTSVAGSPLPLTTGNESLDITIVKHLQYCTQLIQQIVFSSKTPFVARSLLEKLSRQVLVMQKLAAVSDENLGNITSVVEAIPEFHKKLSLLAFWTKCCSPSGVYHSSAARLIKQLEASFARSINKDYPGLAEPVFRTLVSQILDRAEPLLSSSLSSEVITVFQYYSFFTSHGVSDLETYLGQLTRQVAMVQTLQSLRDEKLLQTMSDLAPSNLPAQQEVLRTLALLLTRDDNEVSEAVTLYLAAASKNEHFREKALLYYCEALTKANLQLQKAACLALKSLEATESIKMLVTLCQSDTEEIRTVASETLLSLGEDGRLAYEQLDKFPRDCVKVGGRHGTEVATAF