Protein: Translational regulator orb2 (Q9VSR3)
Organism: Drosophila melanogaster (Fruit fly)
Function: RNA-binding protein involved in translational regulation and required for long-term memory. Required in mushroom body gamma neurons for long-term memory in male courtship. Binds to mRNA 3'-UTRs. In its monomeric form, acts as a translational repressor of genes involved in neuronal growth, synapse formation and protein turnover. In its amyloid-like oligomeric form, acts as a translational activator. The monomeric form reduces poly(A) tail length and destabilizes mRNA while the oligomeric form protects and elongates the poly(A) tail and stabilizes mRNA. Involved in asymmetric cell division in the central nervous system. Plays a role in synapse formation and morphology at neuromuscular junctions by modulating the translation of the tumor suppressor brat. Required for the progression of spermatogenesis through meiosis and for sperm differentiation. During sperm differentiation, required to asymmetrically localize and activate the translation of protein kinase aPKC mRNAs which is necessary for spermatid cyst polarization. Also required during spermatid cyst polarization for localization and translation of its own mRNA.
Subcellular location: Perikaryon {ECO:0000269|PubMed:21900268}. Cell projection, axon {ECO:0000269|PubMed:21900268}. Cell projection, dendrite {ECO:0000269|PubMed:21900268}. Synapse {ECO:0000269|PubMed:22284910, ECO:0000269|PubMed:23083740, ECO:0000269|PubMed:24523662}. Cytoplasm {ECO:0000269|PubMed:21900268, ECO:0000269|PubMed:23209437}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:23209437}. Note=In embryonic and larval nervous system, concentrated in the perikaryon (PubMed:21900268). In the adult central nervous system, localizes to synaptic terminals (PubMed:21900268). In the ovary, localizes to the cytoplasm of nurse cells (PubMed:21900268). Localizes to neuromuscular junctions (PubMed:29105522). In spermatocytes, localizes throughout the cytoplasm with higher levels concentrated in a ring around the nucleus (PubMed:23209437). The oligomeric form is enriched in the synaptic region (PubMed:22284910). {ECO:0000269|PubMed:21900268, ECO:0000269|PubMed:22284910, ECO:0000269|PubMed:29105522}.; SUBCELLULAR LOCATION: [Isoform A]: Synapse {ECO:0000269|PubMed:26095367}.; SUBCELLULAR LOCATION: [Isoform B]: Perikaryon {ECO:0000269|PubMed:26095367}. Cell projection, axon {ECO:0000269|PubMed:26095367}. Synapse {ECO:0000269|PubMed:26095367}.
Interaction partners:
- FI20148p1
- Fragile X messenger ribonucleoprotein 1 homolog
- LD36772p
- GH11843p
- Purine-rich binding protein-alpha, isoform B
- Eukaryotic translation initiation factor 4E1
- Symplekin
- polynucleotide adenylyltransferase
- ATP-dependent RNA helicase me31b
- Poly
Sequence:
MDSLKLPKANSATSSASGSNSNLSGSTSASASAATSPTSSGTAVGGILSGAPKSPPGLGSSTPISVRFNANEESLDDILQSFHHSKHSPSGGASGGGDASPTSNLLGMKNNGLGLVVGNCDSLSSSPSQPQMHAGSASLFGNDEVSLRNNFMQAGGFFNRKSCGGLPNLNLNKPPQLHQQQHQQQHQQHQQHQQQQQLHQHQQQLSPNLSALHHHHQQQQQLRESGGSHSPSSPGGGGGGSPYNGSQAGCSSGGISPIPPQMGVSPKYRRSISFPIKGNSPTAIYGNMHMDGMGSGHMNIPTLSIGNGGGGGSTGMVSAGATGGGDAPYLGNSYGNMMTSNGQMHHGGGLDNSLCDYMRNMSLGGNGGGDGSNSMSLMQDRMRVMGGPKHLSEADAMAIAASGNDPSVYLNALKMGSPSRLSPHSPHSPIQGGNGGNVGDGTARFSRKVFVGGLPPDIDEDEITTSFRRFGPLVVDWPHKAESKSYFPPKGYAFLLFQDESSVQQLIDSCITDEDKLYLCVSSPTIKDKAVQIRPWRLADADYVLDATMSLDPRKTVFVGGVPRPLKAFELAMIMDRLYGGVCYAGIDTDPELKYPKGAGRVAFSNQQSYIAAISARFVQLQHGDIDKRVEVKPYVLDDQMCDECEGQRCGGKFAPFFCANVTCLQYYCEHCWAVIHSRPGREYHKPLVKEGADRPRAVPFRWC