Protein: Interferon-gamma-inducible GTPase 10 (Q0GUM3)
Organism: Mus musculus (Mouse)
Function: Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens by mediating cytosolic release of pathogenic ligands that activate the inflammasomes. Following infection, recruited to the membrane of pathogens in a GBP-dependent manner and mediates disruption of the pathogen membrane, liberating ligands that are detected by inflammasomes, such as lipopolysaccharide (LPS) that activates the non- canonical CASP4/CASP11 inflammasome or double-stranded DNA (dsDNA) that activates the AIM2 inflammasome. Promotes AIM2 and NLRP3 inflammasome activation following A.fumigatus infection by liberating beta-glucan, which directly triggers inflammasome assembly. Promotes NLRP3 inflammasome activation following influenza A virus infection
Subcellular location: Membrane; Multi- pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note=Recruited to the membrane of pathogens in the cytosol in a GBP (Gbp1, Gbp2, Gbp3, Gbp5 ad/or Gbp7)-dependent manner. Recruited to the membrane of pathogen-containing vacuoles: vacuoles may serve as a cellular transport mechanism to deliver Gm12250/Irgb10 to the pathogen membrane (Probable)
Sequence: MGQSSSKPDAKAHNMASSLTEFFKNFKMESKIISKETIDSIQSCIQEGDIQKVISIINAALTDIEKAPLNIAVTGETGAGKSTFINALRGIGHEESESAESGAVETTKDRKKYTHPKFPNVTIWDLPGVGTTNFKPEEYLKKMKFQEYDFFLIISSARFRDNEAQLAEAIKKMKKKFYFVRTKIDSDLWNEKKAKPSSYNREKILEVIRSDCVKNLQNANAASTRVFLVSSFEVAQFDFPSLESTLLEELPAHKRHIFVQCLPTITEPAIDRRRDVLKQTIWLEALKAGASATIPMMSFFNDDIEEFEKILSHYRACFGLDDESLENMAKEWSMSVEELESTIKSPHLLSSEPNESVADKLVKTMEKIFAVTGGFVATGLYFRKSYYMQNYFLDTVTEDAKVLLKKKVFLQDSVDSE
InterPro domains: - IPR030385: IRG-type guanine nucleotide-binding (G) domain (domain) [67-249]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [34-343]
- IPR051515: Immunity-related GTPase (family) [17-412]
- IPR007743: Immunity-related GTPases-like (family) [34-406]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0097367 (carbohydrate derivative binding), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0042802 (identical protein binding), GO:0000166 (nucleotide binding), GO:0032553 (ribonucleotide binding), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0019003 (GDP binding), GO:0032555 (purine ribonucleotide binding), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0017076 (purine nucleotide binding), GO:0016462 (pyrophosphatase activity), GO:0005525 (GTP binding), GO:0019001 (guanyl nucleotide binding), GO:0032561 (guanyl ribonucleotide binding), GO:0017111 (ribonucleoside triphosphate phosphatase activity), GO:0003924 (GTPase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009605 (response to external stimulus), GO:0009607 (response to biotic stimulus), GO:0051707 (response to other organism), GO:0009617 (response to bacterium), GO:0043207 (response to external biotic stimulus)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005773 (vacuole), GO:0005783 (endoplasmic reticulum), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043227 (membrane-bounded organelle), GO:0000323 (lytic vacuole), GO:0030139 (endocytic vesicle), GO:0005776 (autophagosome), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0005770 (late endosome), GO:0005764 (lysosome), GO:0045335 (phagocytic vesicle), GO:0044754 (autolysosome), GO:0097708 (intracellular vesicle), GO:0005767 (secondary lysosome)