Protein: Dihydrofolate reductase (EC 1.5.1.3) (P00375)
Organism: Mus musculus (Mouse)
Function: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA.
Subcellular location: Mitochondrion {ECO:0000269|PubMed:25980602}. Cytoplasm {ECO:0000269|PubMed:25980602}.
Sequence: MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQPELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEYPGVLSEVQEEKGIKYKFEVYEKKD
InterPro domains: - IPR017925: Dihydrofolate reductase conserved site (conserved_site) [16-39]
- IPR001796: Dihydrofolate reductase domain (domain) [4-185]
- IPR012259: Dihydrofolate reductase (family) [5-186]
- IPR024072: Dihydrofolate reductase-like domain superfamily (homologous_superfamily) [2-187]
Interaction partners: - Thymidylate synthase
- Serine hydroxymethyltransferase, cytosolic
- Serine hydroxymethyltransferase, mitochondrial
- C-1-tetrahydrofolate synthase, cytoplasmic
- Folylpolyglutamate synthase, mitochondrial
- Bifunctional purine biosynthesis protein ATIC
- Methionine synthase
- Monofunctional C1-tetrahydrofolate synthase, mitochondrial
- Trifunctional purine biosynthetic protein adenosine-3
- Sepiapterin reductase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016645 (oxidoreductase activity, acting on the CH-NH group of donors), GO:0016646 (oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor), GO:0004146 (dihydrofolate reductase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009058 (biosynthetic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009892 (negative regulation of metabolic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0050794 (regulation of cellular process), GO:0048523 (negative regulation of cellular process), GO:1901615 (organic hydroxy compound metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006066 (alcohol metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0006575 (cellular modified amino acid metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0006082 (organic acid metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0042398 (cellular modified amino acid biosynthetic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0043436 (oxoacid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0051248 (negative regulation of protein metabolic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:0006760 (folic acid-containing compound metabolic process), GO:0046165 (alcohol biosynthetic process), GO:0010468 (regulation of gene expression), GO:1901566 (organonitrogen compound biosynthetic process), GO:0034248 (regulation of amide metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0019751 (polyol metabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0042558 (pteridine-containing compound metabolic process), GO:0010629 (negative regulation of gene expression), GO:0034249 (negative regulation of amide metabolic process), GO:0017148 (negative regulation of translation), GO:0009396 (folic acid-containing compound biosynthetic process), GO:0019752 (carboxylic acid metabolic process), GO:0042559 (pteridine-containing compound biosynthetic process), GO:2000112 (regulation of cellular macromolecule biosynthetic process), GO:0034311 (diol metabolic process), GO:0006417 (regulation of translation), GO:0046173 (polyol biosynthetic process), GO:2000113 (negative regulation of cellular macromolecule biosynthetic process), GO:0046653 (tetrahydrofolate metabolic process), GO:0010608 (post-transcriptional regulation of gene expression), GO:0034312 (diol biosynthetic process), GO:0043648 (dicarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)