Protein: Resuscitation-promoting factor RpfB (EC 3.-.-.-) (P9WG29)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity.
Subcellular location: Cell membrane {ECO:0000255|PROSITE-ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}. Note=Localizes to the septa upon expression in M.smegmatis. {ECO:0000269|PubMed:17919286}.
Sequence: MLRLVVGALLLVLAFAGGYAVAACKTVTLTVDGTAMRVTTMKSRVIDIVEENGFSVDDRDDLYPAAGVQVHDADTIVLRRSRPLQISLDGHDAKQVWTTASTVDEALAQLAMTDTAPAAASRASRVPLSGMALPVVSAKTVQLNDGGLVRTVHLPAPNVAGLLSAAGVPLLQSDHVVPAATAPIVEGMQIQVTRNRIKKVTERLPLPPNARRVEDPEMNMSREVVEDPGVPGTQDVTFAVAEVNGVETGRLPVANVVVTPAHEAVVRVGTKPGTEVPPVIDGSIWDAIAGCEAGGNWAINTGNGYYGGVQFDQGTWEANGGLRYAPRADLATREEQIAVAEVTRLRQGWGAWPVCAARAGAR
InterPro domains: - IPR051933: Bacterial Resuscitation-promoting factor RpfB (family) [1-356]
- IPR007137: Resuscitation-promoting factor, domain of unknown function DUF348 (domain) [27-180]
- IPR023346: Lysozyme-like domain superfamily (homologous_superfamily) [278-361]
- IPR010618: Resuscitation-promoting factor, core lysozyme-like domain (domain) [283-355]
- IPR011098: G5 domain (domain) [192-272]
Interaction partners: - Ribosomal RNA small subunit methyltransferase A
- Peptidoglycan endopeptidase RipA
- Uncharacterized metal-dependent hydrolase TatD
- Diacylglycerol acyltransferase/mycolyltransferase Ag85B
- Antitoxin VapB47
- 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
- Peptidoglycan endopeptidase RipB
- Probable endopeptidase Rv2190c
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0061783 (peptidoglycan muralytic activity), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0016798 (hydrolase activity, acting on glycosyl bonds), GO:0003796 (lysozyme activity), GO:0004553 (hydrolase activity, hydrolyzing O-glycosyl compounds)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0071554 (cell wall organization or biogenesis), GO:0006807 (nitrogen compound metabolic process), GO:0044036 (cell wall macromolecule metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0044248 (cellular catabolic process), GO:0009057 (macromolecule catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0016998 (cell wall macromolecule catabolic process), GO:1901136 (carbohydrate derivative catabolic process), GO:0044265 (cellular macromolecule catabolic process), GO:0006022 (aminoglycan metabolic process), GO:0006026 (aminoglycan catabolic process), GO:0030203 (glycosaminoglycan metabolic process), GO:0006027 (glycosaminoglycan catabolic process), GO:0000270 (peptidoglycan metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005576 (extracellular region)