Protein: Alpha-mannosidase 2 (EC 3.2.1.114) (Golgi alpha-mannosidase II) (AMan II) (Man II) (Mannosidase alpha class 2A member 1) (Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase) (P27046)
Organism: Mus musculus (Mouse)
Function: Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein.
Sequence: MKLSRQFTVFGSAIFCVVIFSLYLMLDRGHLDYPRGPRQEGSFPQGQLSILQEKIDHLERLLAENNEIISNIRDSVINLSESVEDGPRGSPGNASQGSIHLHSPQLALQADPRDCLFASQSGSQPRDVQMLDVYDLIPFDNPDGGVWKQGFDIKYEADEWDHEPLQVFVVPHSHNDPGWLKTFNDYFRDKTQYIFNNMVLKLKEDSSRKFMWSEISYLAKWWDIIDIPKKEAVKSLLQNGQLEIVTGGWVMPDEATPHYFALIDQLIEGHQWLEKNLGVKPRSGWAIDPFGHSPTMAYLLKRAGFSHMLIQRVHYAIKKHFSLHKTLEFFWRQNWDLGSATDILCHMMPFYSYDIPHTCGPDPKICCQFDFKRLPGGRYGCPWGVPPEAISPGNVQSRAQMLLDQYRKKSKLFRTKVLLAPLGDDFRFSEYTEWDLQCRNYEQLFSYMNSQPHLKVKIQFGTLSDYFDALEKAVAAEKKSSQSVFPALSGDFFTYADRDDHYWSGYFTSRPFYKRMDRIMESRIRAAEILYQLALKQAQKYKINKFLSSPHYTTLTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFQSLNSLEKIIGDSAFLLILKDKKLYQSDPSKAFLEMDTKQSSQDSLPQKIIIQLSAQEPRYLVVYNPFEQERHSVVSIRVNSATVKVLSDSGKPVEVQVSAVWNDMRTISQAAYEVSFLAHIPPLGLKVFKILESQSSSSHLADYVLYNNDGLAENGIFHVKNMVDAGDAITIENPFLAIWFDRSGLMEKVRRKEDSRQHELKVQFLWYGTTNKRDKSGAYLFLPDGQGQPYVSLRPPFVRVTRGRIYSDVTCFLEHVTHKVRLYNIQGIEGQSMEVSNIVNIRNVHNREIVMRISSKINNQNRYYTDLNGYQIQPRRTMSKLPLQANVYPMCTMAYIQDAEHRLTLLSAQSLGASSMASGQIEVFMDRRLMQDDNRGLGQGVHDNKITANLFRILLEKRSAVNMEEEKKSPVSYPSLLSHMTSSFLNHPFLPMVLSGQLPSPAFELLSEFPLLQSSLPCDIHLVNLRTIQSKMGKGYSDEAALILHRKGFDCQFSSRGIGLPCSTTQGKMSVLKLFNKFAVESLVPSSLSLMHSPPDAQNMSEVSLSPMEISTFRIRLRWT
InterPro domains: - IPR011013: Galactose mutarotase-like domain superfamily (homologous_superfamily) [621-1147]
- IPR050843: Glycosyl Hydrolase Family 38 (family) [14-1147]
- IPR011330: Glycoside hydrolase/deacetylase, beta/alpha-barrel (homologous_superfamily) [122-498]
- IPR000602: Glycoside hydrolase family 38, N-terminal domain (domain) [166-495]
- IPR015341: Glycoside hydrolase family 38, central domain (domain) [501-602]
- IPR013780: Glycosyl hydrolase, all-beta (homologous_superfamily) [622-1023]
- IPR028995: Glycoside hydrolase families 57/38, central domain superfamily (homologous_superfamily) [501-613]
- IPR011682: Glycosyl hydrolase family 38, C-terminal (domain) [761-965]
- IPR037094: Glycoside hydrolase family 38, central domain superfamily (homologous_superfamily) [500-616]
- IPR027291: Glycoside hydrolase 38, N-terminal domain superfamily (homologous_superfamily) [117-499]
Interaction partners: - Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
- Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
- Alpha-
- Alpha-mannosidase 2x
- alpha-1,2-Mannosidase
- Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
- Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016798 (hydrolase activity, acting on glycosyl bonds), GO:0004553 (hydrolase activity, hydrolyzing O-glycosyl compounds), GO:0016799 (hydrolase activity, hydrolyzing N-glycosyl compounds), GO:0015923 (mannosidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0002376 (immune system process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0070085 (glycosylation), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0043413 (macromolecule glycosylation), GO:1901135 (carbohydrate derivative metabolic process), GO:0036211 (protein modification process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0006486 (protein glycosylation), GO:0043412 (macromolecule modification), GO:0009100 (glycoprotein metabolic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0006491 (N-glycan processing), GO:0006487 (protein N-linked glycosylation), GO:0009101 (glycoprotein biosynthetic process), GO:0018279 (protein N-linked glycosylation via asparagine)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0016020 (membrane), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0005794 (Golgi apparatus), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0000139 (Golgi membrane), GO:0043231 (intracellular membrane-bounded organelle)