Protein: SNF1 protein kinase subunit beta-1 (SNF1-interacting protein 1) (P32578)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Beta subunit of the SNF1 kinase complex, which is required for transcriptional, metabolic, and developmental adaptations in response to glucose limitation. Has a structural role, mediating heterotrimer formation, and a regulatory role, defining carbon source-regulated subcellular location and substrate specificity of the SNF1 kinase complex. Promotes the PKA-regulated relocalization of the SNF1 kinase complex to the vacuolar membrane in response to various types of carbon stress.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:11331606, ECO:0000269|PubMed:14966266}. Vacuole membrane {ECO:0000269|PubMed:11331606, ECO:0000269|PubMed:14966266}; Peripheral membrane protein {ECO:0000269|PubMed:11331606, ECO:0000269|PubMed:14966266}; Cytoplasmic side {ECO:0000269|PubMed:11331606, ECO:0000269|PubMed:14966266}. Note=Resides in the cytosol during growth in glucose and relocalizes to the vacuolar membrane in response to carbon stress. {ECO:0000269|PubMed:14966266}.
Sequence: MGNSPSTQDPSHSTKKEHGHHFHDAFNKDRQGSITSQLFNNRKSTHKRRASHTSEHNGAIPPRMQLLASHDPSTDCDGRMSSDTTIDKGPSHLFKKDYSLSSAADVNDTTLANLTLSDDHDVGAPEEQVKSPSFLSPGPSMATVKRTKSDLDDLSTLNYTMVDETTENERNDKPHHERHRSSIIALKKNLLESSATASPSPTRSSSVHSASLPALTKTDSIDIPVRQPYSKKPSIHAYQYQYLNNDETFSENSQMDKEGNSDSVDAEAGVLQSEDMVLNQSLLQNALKKDMQRLSRVNSSNSMYTAERISHANNNGNIENNTRNKGNAGGSNDDFTAPISATAKMMMKLYGDKTLMERDLNKHHNKTKKAQNKKIRSVSNSRRSSFASLHSLQSRKSILTNGLNLQPLHPLHPIINDNESQYSAPQHREISHHSNSMSSMSSISSTNSTENTLVVLKWKDDGTVAATTEVFIVSTDIASALKEQRELTLDENASLDSEKQLNPRIRMVYDDVHKEWFVPDLFLPAGIYRLQFSINGILTHSNFLPTATDSEGNFVNWFEVLPGYHTIEPFRNEADIDSQVEPTLDEELPKRPELKRFPSSSRKSSYYSAKGVERPSTPFSDYRGLSRSSSINMRDSFVRLKASSLDLMAEVKPERLVYSNEIPNLFNIGDGSTISVKGDSDDVHPQEPPSFTHRVVDCNQDDLFATLQQGGNIDAETAEAVFLSRYPVPDLPIYLNSSYLNRILNQSNQNSESHERDEGAINHIIPHVNLNHLLTSSIRDEIISVACTTRYEGKFITQVVYAPCYYKTQKSQISN
InterPro domains: - IPR037256: ASC domain superfamily (homologous_superfamily) [687-804]
- IPR006828: Association with the SNF1 complex (ASC) domain (domain) [684-804]
- IPR013783: Immunoglobulin-like fold (homologous_superfamily) [498-560]
- IPR050827: CRP1/MDG1 DNA-binding & kinase subunit (family) [313-814]
- IPR032640: AMP-activated protein kinase, glycogen-binding domain (domain) [505-561]
- IPR014756: Immunoglobulin E-set (homologous_superfamily) [502-560]
Interaction partners: - 5'-AMP-activated protein kinase subunit gamma
- Carbon catabolite-derepressing protein kinase
- SNF1 protein kinase subunit beta-2
- SNF1 protein kinase subunit beta-3
- SNF1-activating kinase 1
- Regulatory protein MIG1
- Protein SIP4
- Regulatory protein CAT8
- Serine/threonine-protein kinase TOS3
- Serine/threonine-protein kinase ELM1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0060090 (molecular adaptor activity), GO:0003824 (catalytic activity), GO:0030674 (protein-macromolecule adaptor activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0004672 (protein kinase activity), GO:0140767 (enzyme-substrate adaptor activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity), GO:0004679 (AMP-activated protein kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0009605 (response to external stimulus), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0071840 (cellular component organization or biogenesis), GO:0006950 (response to stress), GO:0061919 (process utilizing autophagic mechanism), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0009991 (response to extracellular stimulus), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0042594 (response to starvation), GO:0035556 (intracellular signal transduction), GO:0033554 (cellular response to stress), GO:0044248 (cellular catabolic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0016043 (cellular component organization), GO:0031668 (cellular response to extracellular stimulus), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0051128 (regulation of cellular component organization), GO:0071496 (cellular response to external stimulus), GO:0043170 (macromolecule metabolic process), GO:0006914 (autophagy), GO:0016236 (macroautophagy), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0006412 (translation), GO:0006518 (peptide metabolic process), GO:0009267 (cellular response to starvation), GO:0031667 (response to nutrient levels), GO:0000165 (MAPK cascade), GO:0043604 (amide biosynthetic process), GO:0033043 (regulation of organelle organization), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0043933 (protein-containing complex organization), GO:0031669 (cellular response to nutrient levels), GO:0022411 (cellular component disassembly), GO:0010467 (gene expression), GO:0019932 (second-messenger-mediated signaling), GO:0043043 (peptide biosynthetic process), GO:0097352 (autophagosome maturation), GO:0032984 (protein-containing complex disassembly), GO:0006415 (translational termination), GO:0042149 (cellular response to glucose starvation), GO:0006415 (translational termination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0005622 (intracellular anatomical structure), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0140535 (intracellular protein-containing complex), GO:0031588 (nucleotide-activated protein kinase complex), GO:1990234 (transferase complex), GO:0005886 (plasma membrane), GO:0061695 (transferase complex, transferring phosphorus-containing groups), GO:1902911 (protein kinase complex)