Protein: 2,7-dihydroxy-5-methyl-1-naphthoate 7-O-methyltransferase (EC 2.1.1.303) (Neocarzinostatin O-methyltransferase) (Neocarzinostatin biosynthesis protein B1) (Q84HC8)
Organism: Streptomyces carzinostaticus
Function: S-adenosyl-L-methionine-dependent O-methyltransferase that catalyzes regiospecific methylation at the 7-hydroxy group of 2,7-dihydroxy-5-methyl-1-naphthoate in the biosynthesis of the naphthoate moiety of the neocarzinostatin chromophore. Also recognizes other dihydroxynaphthoate as substrates and catalyzes their regiospecific O-methylation. The carboxylate and its ortho-hydroxy groups of the substrate appear to be crucial for NcsB1 substrate recognition and binding, and O-methylation takes place only at the free hydroxy group of these dihydroxynaphthoic acids.
Sequence: MGKRAAHIGLRALADLATPMAVRVAATLRVADHIAAGHRTAAEIASAAGAHADSLDRLLRHLVAVGLFTRDGQGVYGLTEFGEQLRDDHAAGKRKWLDMNSAVGRGDLGFVELAHSIRTGQPAYPVRYGTSFWEDLGSDPVLSASFDTLMSHHLELDYTGIAAKYDWAALGHVVDVGGGSGGLLSALLTAHEDLSGTVLDLQGPASAAHRRFLDTGLSGRAQVVVGSFFDPLPAGAGGYVLSAVLHDWDDLSAVAILRRCAEAAGSGGVVLVIEAVAGDEHAGTGMDLRMLTYFGGKERSLAELGELAAQAGLAVRAAHPISYVSIVEMTAL
InterPro domains: - IPR036390: Winged helix DNA-binding domain superfamily (homologous_superfamily) [12-89]
- IPR012967: O-methyltransferase, dimerisation domain (domain) [15-83]
- IPR029063: S-adenosyl-L-methionine-dependent methyltransferase superfamily (homologous_superfamily) [111-322]
- IPR001077: O-methyltransferase, C-terminal domain (domain) [110-313]
- IPR016461: O-methyltransferase-like (family) [3-332]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [1-99]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0008168 (methyltransferase activity), GO:0008171 (O-methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0016999 (antibiotic metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:0017000 (antibiotic biosynthetic process), GO:0042180 (cellular ketone metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:0044249 (cellular biosynthetic process), GO:0044597 (daunorubicin metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0030638 (polyketide metabolic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0042181 (ketone biosynthetic process), GO:1901661 (quinone metabolic process), GO:1901657 (glycosyl compound metabolic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0030648 (aminoglycoside antibiotic biosynthetic process), GO:0030639 (polyketide biosynthetic process), GO:1901659 (glycosyl compound biosynthetic process), GO:0016137 (glycoside metabolic process), GO:1901663 (quinone biosynthetic process), GO:0030647 (aminoglycoside antibiotic metabolic process), GO:0016138 (glycoside biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)