Protein: Probable diacyglycerol O-acyltransferase tgs3 (TGS3) (EC 2.3.1.20) (Probable triacylglycerol synthase tgs3) (P9WKC5)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis.
Sequence: MVTRLSASDASFYQLENTATPMYVGLLLILRRPRAGLSYEALLETVEQRLPQIPRYRQKVQEVKLGLARPVWIDDRDFDITYHVRRSALPSPGSDEQLHELIARLAARPLDKSRPLWEMYLVEGLEKNRIALYTKSHQALINGVTALAIGHVIADRTRRPPAFPEDIWVPERDPGTTRLLLRAVGDWLVRPGAQLQAVGSAVAGLVTNSGQLVETGRKVLDIARTVARGTAPSSPLNATVSRNRRFTVARASLDDYRTVRARYDCDSTTWC
InterPro domains: - IPR004255: O-acyltransferase, WSD1-like, N-terminal (domain) [5-270]
- IPR045034: O-acyltransferase WSD1-like (family) [37-262]
Interaction partners: - Possible triacylglycerol synthase
- Possible multi-functional enzyme with acyl-CoA-reductase activity AcrA1
- Conserved protein
- Diacylglycerol kinase
- Triacylglycerol lipase
- Fatty acyl-CoA reductase Rv0547c
- GDP-polyphosphate phosphotransferase
- Uncharacterized protein Rv1414
- Uncharacterized protein Rv2308
- NADPH oxidoreductase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016746 (acyltransferase activity), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups), GO:0008374 (O-acyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009987 (cellular process), GO:0009605 (response to external stimulus), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0009607 (response to biotic stimulus), GO:0071704 (organic substance metabolic process), GO:0044403 (biological process involved in symbiotic interaction), GO:0051707 (response to other organism), GO:0044238 (primary metabolic process), GO:0051701 (biological process involved in interaction with host), GO:0010035 (response to inorganic substance), GO:1901576 (organic substance biosynthetic process), GO:0043207 (response to external biotic stimulus), GO:0052173 (response to defenses of other organism), GO:0006629 (lipid metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0075136 (response to host), GO:0044249 (cellular biosynthetic process), GO:1901700 (response to oxygen-containing compound), GO:0010035 (response to inorganic substance), GO:0006629 (lipid metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:1902074 (response to salt), GO:0010038 (response to metal ion), GO:0052200 (response to host defenses), GO:0006643 (membrane lipid metabolic process), GO:1903509 (liposaccharide metabolic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0046467 (membrane lipid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0052572 (response to host immune response), GO:0006664 (glycolipid metabolic process), GO:0052572 (response to host immune response), GO:0009247 (glycolipid biosynthetic process), GO:0052553 (modulation by symbiont of host immune response)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)