Protein: Transcription factor HES-1 (Hairy and enhancer of split 1) (Hairy-like protein) (RHL) (Q04666)
Organism: Rattus norvegicus (Rat)
Function: Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage (By similarity).
Subcellular location: Nucleus.
Sequence: MPADIMEKNSSSPVAATPASVNTTPDKPKTASEHRKSSKPIMEKRRRARINESLSQLKTLILDALKKDSSRHSKLEKADILEMTVKHLRNLQRAQMTAALSTDPSVLGKYRAGFSECMNEVTRFLSTCEGVNTEVRTRLLGHLANCMTQINAMTYPGQAHPALQAPPPPPPSGPGGPQHAPFAPPPPLVPIPGGAAPPPGSAPCKLGSQAGEAAKVFGGFQVVPAPDGQFAFLIPNGAFAHSGPVIPVYTSNSGTSVGPNAVSPSSGSSLTADSMWRPWRN
InterPro domains: - IPR011598: Myc-type, basic helix-loop-helix (bHLH) domain (domain) [34-97]
- IPR003650: Orange domain (domain) [108-152]
- IPR036638: Helix-loop-helix DNA-binding domain superfamily (homologous_superfamily) [25-95]
- IPR050370: Hairy and Enhancer of Split/HEY-related (family) [33-279]
Interaction partners: - FA complementation group G
- E3 ubiquitin-protein ligase FANCL
- Fanconi anemia group F protein
- FA complementation group A
- FA complementation group E
- Fanconi anemia group C protein homolog
- Neurogenin 3
- Transcription factor HES-5
- Fanconi anemia group D2 protein homolog
- FA complementation group I
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:0003700 (DNA-binding transcription factor activity), GO:0005515 (protein binding), GO:0008134 (transcription factor binding), GO:0000981 (DNA-binding transcription factor activity, RNA polymerase II-specific), GO:0001221 (transcription coregulator binding), GO:0140297 (DNA-binding transcription factor binding), GO:0001222 (transcription corepressor binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0032501 (multicellular organismal process), GO:0050793 (regulation of developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0051239 (regulation of multicellular organismal process), GO:0007272 (ensheathment of neurons), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:2000026 (regulation of multicellular organismal development), GO:0008366 (axon ensheathment), GO:0035556 (intracellular signal transduction), GO:0048731 (system development), GO:0044057 (regulation of system process), GO:0045595 (regulation of cell differentiation), GO:0007166 (cell surface receptor signaling pathway), GO:0007399 (nervous system development), GO:0042552 (myelination), GO:0031644 (regulation of nervous system process), GO:0032292 (peripheral nervous system axon ensheathment), GO:0021782 (glial cell development), GO:0010001 (glial cell differentiation), GO:0051960 (regulation of nervous system development), GO:0007219 (Notch signaling pathway), GO:0042063 (gliogenesis), GO:0014037 (Schwann cell differentiation), GO:0022011 (myelination in peripheral nervous system), GO:0014044 (Schwann cell development)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)