Protein: Ubiquitin-like-specific protease ESD4 (EC 3.4.22.68) (Protein EARLY IN SHORT DAYS 4) (AtESD4) (Q94F30)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMOs to their mature forms and deconjugation of SUMO from targeted proteins. Cleaves precursors of SUM1 and SUM2, but not of SUM3 or SUM5. Able to release SUM1 and SUM2 from conjugates, but unable to cleave SUM3. Acts predominantly as an isopeptidase, cleaving SUMO-conjugated proteins better than SUMO peptides. Plays an important role in the control of flowering time.
Subcellular location: Nucleus membrane {ECO:0000269|PubMed:14507998, ECO:0000269|PubMed:17513499}; Peripheral membrane protein {ECO:0000269|PubMed:14507998, ECO:0000269|PubMed:17513499}. Note=The nuclear envelope localization is independent of the presence of the nuclear pore anchor NUA.
Sequence: MGAVAINRKRSDESFNFINQQSTNPLRNSPYFQASKKRRFSFAMSEDSGKPASSNPTISRISRYPDAKAPLRREIHAPSRGILRYGKAKSNDYCEKDANFFVRKYDDAKRSALEALRFVNKGKDFVDLGDEVEKEEVVSDDSSVQAIEVIDCDDDEEKKNLQPSFSSGVTDVKKGENFRVEDTSMMLDSLSLDRDVDNDASSLEAYRKLMQSAEKRNSKLEALGFEIVLNEKKLSLLRQSRPKTVEKRVEVPREPFIPLTEDEEAEVYRAFSGRNRRKVLATHENSNIDITGEVLQCLTPSAWLNDEVINVYLELLKERETREPKKYLKCHYFNTFFYKKLVSDSGYNFKAVRRWTTQRKLGYALIDCDMIFVPIHRGVHWTLAVINNRESKLLYLDSLNGVDPMILNALAKYMGDEANEKSGKKIDANSWDMEFVEDLPQQKNGYDCGMFMLKYIDFFSRGLGLCFSQEHMPYFRLRTAKEILRLRAD
InterPro domains: - IPR038765: Papain-like cysteine peptidase superfamily (homologous_superfamily) [255-487]
- IPR003653: Ulp1 protease family, C-terminal catalytic domain (domain) [288-483]
Interaction partners: - Small ubiquitin-related modifier 1
- Small ubiquitin-related modifier 2
- Small ubiquitin-related modifier 3
- Small ubiquitin-related modifier 5
- Putative small ubiquitin-related modifier 7
- Putative small ubiquitin-related modifier 4
- SUMO-conjugating enzyme SCE1
- Putative small ubiquitin-related modifier 8
- Putative small ubiquitin-related modifier 6
- E3 SUMO-protein ligase SIZ1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0008233 (peptidase activity), GO:0008234 (cysteine-type peptidase activity), GO:0019783 (ubiquitin-like protein peptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0043170 (macromolecule metabolic process), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0006508 (proteolysis), GO:0070646 (protein modification by small protein removal), GO:0070647 (protein modification by small protein conjugation or removal), GO:0018193 (peptidyl-amino acid modification), GO:0018205 (peptidyl-lysine modification), GO:0016926 (protein desumoylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0031967 (organelle envelope), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0043228 (non-membrane-bounded organelle), GO:0009526 (plastid envelope), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0009941 (chloroplast envelope), GO:0005634 (nucleus)