Protein: General transcription factor IIE subunit 1 (General transcription factor IIE 56 kDa subunit) (Transcription initiation factor IIE subunit alpha) (TFIIE-alpha) (P29083)
Organism: Homo sapiens (Human)
Function: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Subcellular location: Nucleus {ECO:0000269|PubMed:1956398, ECO:0000269|PubMed:7651391}.
Sequence: MADPDVLTEVPAALKRLAKYVIRGFYGIEHALALDILIRNSCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVETAADGKTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERDSTNRASFKCPVCSSTFTDLEANQLFDPMTGTFRCTFCHTEVEEDESAMPKKDARTLLARFNEQIEPIYALLRETEDVNLAYEILEPEPTEIPALKQSKDHAATTAGAASLAGGHHREAWATKGPSYEDLYTQNVVINMDDQEDLHRASLEGKSAKERPIWLRESTVQGAYGSEDMKEGGIDMDAFQEREEGHAGPDDNEEVMRALLIHEKKTSSAMAGSVGAAAPVTAANGSDSESETSESDDDSPPRPAAVAVHKREEDEEEDDEFEEVADDPIVMVAGRPFSYSEVSQRPELVAQMTPEEKEAYIAMGQRMFEDLFE
InterPro domains: - IPR002853: Transcription initiation factor IIE subunit alpha, N-terminal (domain) [28-175]
- IPR024550: TFIIEalpha/SarR/Rpc3 HTH domain (domain) [16-123]
- IPR021600: Transcription factor TFIIE alpha subunit, C-terminal (domain) [354-439]
- IPR017919: Transcription factor TFE/TFIIEalpha HTH domain (domain) [14-104]
- IPR039997: Transcription factor E (family) [8-436]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [113-174]
- IPR013137: Zinc finger, TFIIB-type (domain) [127-175]
Interaction partners: - Transcription initiation factor IIB
- General transcription factor IIH subunit 1
- General transcription factor IIF subunit 1
- General transcription and DNA repair factor IIH helicase subunit XPD
- TATA-box-binding protein
- Transcription initiation factor IIE subunit beta
- General transcription factor IIF subunit 2
- General transcription factor IIH subunit 4
- General transcription and DNA repair factor IIH helicase/translocase subunit XPB
- DNA-directed RNA polymerase II subunit RPB1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0044249 (cellular biosynthetic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0043933 (protein-containing complex organization), GO:0010467 (gene expression), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0016070 (RNA metabolic process), GO:0034367 (protein-containing complex remodeling), GO:0071824 (protein-DNA complex subunit organization), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006352 (DNA-templated transcription initiation), GO:0006351 (DNA-templated transcription), GO:0006367 (transcription initiation at RNA polymerase II promoter), GO:0006366 (transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0032993 (protein-DNA complex), GO:0140535 (intracellular protein-containing complex), GO:1902494 (catalytic complex), GO:0140513 (nuclear protein-containing complex), GO:0005667 (transcription regulator complex), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0030880 (RNA polymerase complex), GO:1990234 (transferase complex), GO:0043229 (intracellular organelle), GO:0090575 (RNA polymerase II transcription regulator complex), GO:0005739 (mitochondrion), GO:0043233 (organelle lumen), GO:0055029 (nuclear DNA-directed RNA polymerase complex), GO:0016591 (RNA polymerase II, holoenzyme), GO:0043227 (membrane-bounded organelle), GO:0000428 (DNA-directed RNA polymerase complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0061695 (transferase complex, transferring phosphorus-containing groups), GO:0070013 (intracellular organelle lumen), GO:0005634 (nucleus), GO:0031981 (nuclear lumen)