Protein: PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM (P37349)
Organism: Escherichia coli (strain K12)
Function: Component of the dihydroxyacetone kinase complex, which is responsible for the phosphoenolpyruvate (PEP)-dependent phosphorylation of dihydroxyacetone. DhaM serves as the phosphoryl donor. Is phosphorylated by phosphoenolpyruvate in an EI- and HPr-dependent reaction, and a phosphorelay system on histidine residues finally leads to phosphoryl transfer to DhaL and dihydroxyacetone
Sequence: MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAVVIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTLTVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASELLQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG
InterPro domains: - IPR039643: Dihydroxyacetone kinase phosphotransferase subunit DhaM (family) [1-472]
- IPR036618: PtsI, HPr-binding domain superfamily (homologous_superfamily) [265-388]
- IPR036662: Phosphotransferase system, mannose-type IIA component superfamily (homologous_superfamily) [1-132]
- IPR008731: Phosphotransferase system, enzyme I N-terminal (domain) [258-368]
- IPR008279: PEP-utilising enzyme, mobile domain (domain) [394-465]
- IPR035895: HPr-like superfamily (homologous_superfamily) [156-242]
- IPR012844: Dihydroxyacetone kinase phosphotransferase subunit, N-terminal domain (domain) [2-127]
- IPR004701: Phosphotransferase system, mannose-type IIA component (domain) [1-135]
- IPR001020: Phosphotransferase system, HPr histidine phosphorylation site (ptm) [167-174]
- IPR000032: Phosphocarrier protein HPr-like (domain) [155-242]
- IPR036637: Phosphohistidine domain superfamily (homologous_superfamily) [355-463]
Interaction partners: - PEP-dependent dihydroxyacetone kinase, ADP-binding subunit DhaL
- PEP-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK
- Glycerol dehydrogenase
- PTS system glucose-specific EIIA component
- PTS system glucose-specific EIIA component
- PTS system glucose-specific EIIA component
- Multiphosphoryl transfer protein 2
- Multiphosphoryl transfer protein 1
- PTS-dependent dihydroxyacetone kinase operon regulatory protein
- Aerobic glycerol-3-phosphate dehydrogenase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0005975 (carbohydrate metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0033554 (cellular response to stress), GO:0006082 (organic acid metabolic process), GO:0006793 (phosphorus metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0046835 (carbohydrate phosphorylation), GO:0006974 (cellular response to DNA damage stimulus), GO:0043436 (oxoacid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0016310 (phosphorylation), GO:0032787 (monocarboxylic acid metabolic process), GO:0006090 (pyruvate metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005829 (cytosol), GO:1902494 (catalytic complex), GO:0005622 (intracellular anatomical structure), GO:0005737 (cytoplasm), GO:1990234 (transferase complex)