Protein: Neutral amino acid transporter 9 (Q08BA4)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Lysosomal amino acid transporter involved in the activation of mTORC1 in response to amino acid levels. Probably acts as an amino acid sensor of the Rag GTPases and Ragulator complexes, 2 complexes involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Following activation by amino acids, the Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. SLC38A9 mediates transport of amino acids with low capacity and specificity with a slight preference for polar amino acids (By similarity). Acts as an arginine sensor. Following activation by arginine binding, mediates transport of L-glutamine, leucine and tyrosine with high efficiency, and is required for the efficient utilization of these amino acids after lysosomal protein degradation. However, the transport mechanism is not well defined and the role of sodium is not clear. Guanine exchange factor (GEF) that, upon arginine binding, stimulates GDP release from RRAGA and therefore activates the Rag GTPase heterodimer and the mTORC1 pathway in response to nutrient sufficiency (By similarity)
Subcellular location: Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein
Sequence: MDEDSKPLLGSVPTGDYYTDSLDPKQRRPFHVEPRNIVGEDVQERVSAEAAVLSSRVHYYSRLTGSSDRLLAPPDHVIPSHEDIYIYSPLGTAFKVQGGDSPIKNPSIVTIFAIWNTMMGTSILSIPWGIKQAGFTLGIIIIVLMGLLTLYCCYRVLKSTKSIPYVDTSDWEFPDVCKYYFGGFGKWSSLVFSLVSLIGAMVVYWVLMSNFLFNTGKFIFNYVHNVNTSDAFGTNGTERVICPYPDVDPHGNSSTSLYSGSDNSTGLEFDHWWSKTNTIPFYLILLLLPLLNFRSASFFARFTFLGTISVIYLIFLVTYKAIQLGFHLEFHWFDSSMFFVPEFRTLFPQLSGVLTLAFFIHNCIITLMKNNKHQENNVRDLSLAYLLVGLTYLYVGVLIFAAFPSPPLSKECIEPNFLDNFPSSDILVFVARTFLLFQMTTVYPLLGYLVRVQLMGQIFGNHYPGFLHVFVLNVFVVGAGVLMARFYPNIGSIIRYSGALCGLALVFVLPSLIHMVSLKRRGELRWTSTLFHGFLILLGVANLLGQFFM
InterPro domains: - IPR013057: Amino acid transporter, transmembrane domain (domain) [110-539]
Interaction partners: - Ragulator complex protein LAMTOR2
- Ragulator complex protein LAMTOR1
- Ragulator complex protein LAMTOR3
- Ragulator complex protein LAMTOR5
- Ragulator complex protein LAMTOR4
- Folliculin-interacting protein 2
- Folliculin
- Ras-related GTP-binding protein
- Amino acid transporter
- Amino acid transporter
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005215 (transporter activity), GO:0140299 (small molecule sensor activity), GO:0036094 (small molecule binding), GO:0022857 (transmembrane transporter activity), GO:0043167 (ion binding), GO:0005342 (organic acid transmembrane transporter activity), GO:0043177 (organic acid binding), GO:0043168 (anion binding), GO:0043169 (cation binding), GO:0008514 (organic anion transmembrane transporter activity), GO:0046943 (carboxylic acid transmembrane transporter activity), GO:0031406 (carboxylic acid binding), GO:0016597 (amino acid binding), GO:0015171 (amino acid transmembrane transporter activity), GO:0015658 (branched-chain amino acid transmembrane transporter activity), GO:0015179 (L-amino acid transmembrane transporter activity), GO:0015175 (neutral L-amino acid transmembrane transporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0051234 (establishment of localization), GO:0006810 (transport), GO:0071702 (organic substance transport), GO:0071705 (nitrogen compound transport), GO:0006865 (amino acid transport), GO:0015849 (organic acid transport), GO:0015711 (organic anion transport), GO:0015804 (neutral amino acid transport), GO:0046942 (carboxylic acid transport), GO:0015803 (branched-chain amino acid transport), GO:0015820 (leucine transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)