Protein: Serine palmitoyltransferase 2 (SPT 2) (EC 2.3.1.50) (Long chain base biosynthesis protein 2) (Q09925)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Catalytic subunit of serine palmitoyltransferase (SPT), which catalyzes the committed step in the synthesis of sphingolipids, the condensation of serine with palmitoyl CoA to form the long chain base 3-ketosphinganine.
Subcellular location: Cytoplasm {ECO:0000250}. Endoplasmic reticulum {ECO:0000269|PubMed:16823372}. Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
Sequence: MAQADFVSPTSIDVSEKKEVEFHKKVDHVENPPLSTESAKLEAEEVAAEKLNSEHLLENEFAPITDPTHRRVSKNPDGAELFQFEDEPSYYYVVATYLTYLVLIIIGHVRDFFGKRFHKDDYKYLKDNDGYAPLYNHFDNFYVRRLQHRINDCFSRPTMGVPGRVIRLMNRYSTDSNSTFKLTGDTSLALNVSSYNYLGFAQSHGPCATKVEEAMQKYGLSTCSSNAICGTYGLHKEVEELTANFVGKPAALVFSQGFSTNATVFSTLMCPGSLIISDELNHTSIRFGARLSGANIRVYKHNDMTDLERVLREVISQGQPRTHRPYSKILVVIEGLYSMEGNFCDLPKVVELKNRYKFYLFIDEAHSIGAIGPRGGGICDYFGISTDHVDILMGTFTKSFGAAGGYISATPNIINKLRVTNPGYVYAESMSPAVLAQIKSSFLEIMDNSPTSAGLERIERLAFNSRYIRLGLKRLGFIIFGNDDSPVVPLLLYNPGKINAFSHEMLKRGIAVVVVGYPACPLLTSRVRFCFSASHNKADMDYFLRACDEVGEKLQLKFSTGAAGEDVGKTNVEKMKKNQGWFKPPRWKIEDVLKHGVHDALTQ
InterPro domains: - IPR001917: Aminotransferase, class-II, pyridoxal-phosphate binding site (binding_site) [395-404]
- IPR015421: Pyridoxal phosphate-dependent transferase, major domain (homologous_superfamily) [204-454]
- IPR050087: 8-amino-7-oxononanoate synthase class-II (family) [96-550]
- IPR004839: Aminotransferase, class I/classII, large domain (domain) [190-546]
- IPR015424: Pyridoxal phosphate-dependent transferase (homologous_superfamily) [136-554]
- IPR015422: Pyridoxal phosphate-dependent transferase, small domain (homologous_superfamily) [189-547]
Interaction partners: - Serine palmitoyltransferase 1
- PIGF/3-ketodihydrosphingosine reductase fusion protein
- Uncharacterized protein C119.09c
- Sphingolipid delta
- Serine/threonine-protein kinase tor1
- Serine/threonine-protein kinase tor2
- Tryptophan synthase
- Target of rapamycin complex 1 subunit mip1
- Target of rapamycin complex subunit wat1
- Serine racemase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016746 (acyltransferase activity), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups), GO:0016408 (C-acyltransferase activity), GO:0016409 (palmitoyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006629 (lipid metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006665 (sphingolipid metabolic process), GO:0046467 (membrane lipid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0006643 (membrane lipid metabolic process), GO:0030148 (sphingolipid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)