Protein: Plasma membrane ascorbate-dependent reductase CYBRD1 (EC 7.2.1.3) (Cytochrome b reductase 1) (Duodenal cytochrome b) (Q925G2)
Organism: Mus musculus (Mouse)
Function: Plasma membrane reductase that uses cytoplasmic ascorbate as an electron donor to reduce extracellular Fe(3+) into Fe(2+). Probably functions in dietary iron absorption at the brush border of duodenal enterocytes by producing Fe(2+), the divalent form of iron that can be transported into enterocytes. It is also able to reduce extracellular monodehydro-L-ascorbate and may be involved in extracellular ascorbate regeneration by erythrocytes in blood. May also act as a ferrireductase in airway epithelial cells (By similarity). May also function as a cupric transmembrane reductase.
Subcellular location: Cell membrane {ECO:0000250|UniProtKB:Q53TN4}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q53TN4}. Apical cell membrane {ECO:0000305|PubMed:11230685, ECO:0000305|PubMed:12547225}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q53TN4}. Note=Localized at the brush border of duodenal cells. {ECO:0000269|PubMed:11230685, ECO:0000269|PubMed:12547225}.
Sequence: MAMEGYRGFLGLLVSALLVGFLSVIFVLIWVLHFREGLGWNGSGLEFNWHPVLAVTGFVFIQGIAIIVYRLPWTWKCSKLLMKSIHAGLNAVAAILAIISVVAVFEYHNVQKVPHMYSLHSWVGLTALILYIQQLVVGFFVFLLPWAPPSLRAIVMPIHVYSGLLLFGTVIATVLMGVTEKLFFVLKHPSYHSFPPEGVFTNTLGLLILVFGALIFWIVTRPQWKRPREPGSVPLQLNGGNAECRMEGAIAISSAHSMDAADPADAESSSEGAARKRTLGLADSGQRSTM
InterPro domains: - IPR043205: Cytochrome b561/Cytochrome b reductase 1-like (family) [12-227]
- IPR006593: Cytochrome b561/ferric reductase transmembrane (domain) [15-220]
Interaction partners: - Natural resistance-associated macrophage protein 2
- Ferroportin
- Hepcidin
- Hephaestin
- Hepcidin-2
- Metalloreductase STEAP3
- Hereditary hemochromatosis protein homolog
- Transferrin receptor protein 1
- Transferrin receptor protein 2
- Proton-coupled folate transporter
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity), GO:0016491 (oxidoreductase activity), GO:0005515 (protein binding), GO:0042802 (identical protein binding), GO:0016722 (oxidoreductase activity, acting on metal ions), GO:0022804 (active transmembrane transporter activity), GO:0015399 (primary active transmembrane transporter activity), GO:0015453 (oxidoreduction-driven active transmembrane transporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0042592 (homeostatic process), GO:0009987 (cellular process), GO:0048878 (chemical homeostasis), GO:0051641 (cellular localization), GO:0019725 (cellular homeostasis), GO:0051234 (establishment of localization), GO:0050801 (monoatomic ion homeostasis), GO:0055082 (intracellular chemical homeostasis), GO:0006810 (transport), GO:0051649 (establishment of localization in cell), GO:0098771 (inorganic ion homeostasis), GO:0098657 (import into cell), GO:0055080 (monoatomic cation homeostasis), GO:0033212 (iron import into cell), GO:0006873 (intracellular monoatomic ion homeostasis), GO:0006811 (monoatomic ion transport), GO:0055072 (iron ion homeostasis), GO:0006812 (monoatomic cation transport), GO:0006879 (intracellular iron ion homeostasis), GO:0030003 (intracellular monoatomic cation homeostasis), GO:0030001 (metal ion transport), GO:0000041 (transition metal ion transport), GO:0006826 (iron ion transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)