Protein: Ent-kaurene synthase 1, chloroplastic (IeKS1) (EC 4.2.3.19) (A0A3G1QTS7)
Organism: Isodon eriocalyx (Plectranthus eriocalyx)
Function: Involved in the biosynthesis of ent-kaurene diterpenoids natural products such as oridonin, miltiradiene, eriocalyxin B and nezukol, known to exhibit antitumor, anti-inflammatory and antibacterial activities, and in the production of gibberellins phytohormones. Catalyzes the conversion of ent-copalyl diphosphate (ent-CPP) to ent-kaurene.
Subcellular location: Plastid, chloroplast {ECO:0000255}.
Sequence: MSLLLSNSVLVGPKFRSSRISHASASLDIGLQRATSPQNASVPTCFEETKGRIAKLFHKNELSVSTYDTAWVAMVPSPTSSEEPCFPACLNWLLENQCHDGSWARPHHHHMLKKDVLSSTLACILALKKWGVGEEQINRGLHFVELNFASATEKGQITPMGFDIIFPAMLDNARGLSLNLQLEPTMLNDLIYKRDLELKRCNQSNSAEKEVYWAHIAEGMGKLHDWESVMKYQRKNGSLFNCPSTTAAAFTALRNSDCLNYLCLALEKFGSAVPAVYPLDIYSQLCTVGNLERLGISRYFLTEIQSVLDETYRSWLQGDEEIFMDASTCALAFRTLRMNGYNVTSDPITKILQECFSSSFRGNMTDNNTTLEIYRASELILYPEERDLVQHNLRLKTFLEQELSSNGFIQSCQLGRNINAEVNQAIEYPFYAIMDRMAKRKNIENYNIDNTRILKTSYRSPNFGNKDFLSLSVEDFNRCQVIHREELRELERWVIENRLDELKFARSKAAYCYFSAAATIFSPELSDARMSWAKNGVLTTVVDDFFDVGGSVEELKNLIQLVELWDVDVSTQCCSPNVQIIFSALKHTICEIADKGFKLQGRSITDHIISIWLDLLYSMMKETELGIDKSFPTMDEYMSNAYVSFALGPIVLPALYLVGPKLSEEMVNHSEYHTLFKLMSTCGRLLNDIRGYERELKDGKISAVSLYIMNNGGEITTEAAISEMRSWIERDRRELLRLVLEENKSVLPKACKKLFWHMCTVVHVFYSKDDGFTSLNLHGVVNAIINEPIVLNQF
InterPro domains: - IPR008930: Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid (homologous_superfamily) [66-449]
- IPR005630: Terpene synthase, metal-binding domain (domain) [499-733]
- IPR001906: Terpene synthase, N-terminal domain (domain) [225-422]
- IPR008949: Isoprenoid synthase domain superfamily (homologous_superfamily) [465-793]
- IPR050148: Terpene synthase-like (family) [47-788]
- IPR044814: Terpene cyclases, class 1, plant (family) [275-788]
- IPR036965: Terpene synthase, N-terminal domain superfamily (homologous_superfamily) [56-476]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016829 (lyase activity), GO:0016835 (carbon-oxygen lyase activity), GO:0016838 (carbon-oxygen lyase activity, acting on phosphates), GO:0010333 (terpene synthase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0120252 (hydrocarbon metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0006720 (isoprenoid metabolic process), GO:0042214 (terpene metabolic process), GO:0008299 (isoprenoid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0006721 (terpenoid metabolic process), GO:0016114 (terpenoid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle)