Protein: Deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) (EC 3.6.1.23) (PPAR-interacting protein 4) (PIP4) (dUTP pyrophosphatase) (P70583)
Organism: Rattus norvegicus (Rat)
Function: Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis (By similarity). Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor. Essential for embryonic development (By similarity).
Subcellular location: Cytoplasm {ECO:0000269|PubMed:8910358}. Nucleus {ECO:0000269|PubMed:8910358}. Note=Binding to PPAR induces translocation to the nucleus. {ECO:0000269|PubMed:8910358}.
Sequence: MPLLCALLRSRLQPSLLRSLTLTSAQSLSCGGSRGVRTWSARTGPGACADPAVSVSKRARAEDDASLRFVRLSEHATAPTRGSARAAGYDLYSAYDYTIPSMEKALVKTDIQIAVPSGCYGRVAPRSGLAVKHFIDVGAGVIDEDYRGNVGVVLFNFGKEKFEVKKGDRIAQLICERILYPDLEEVQTLDNTERGSGGFGSTGKN
InterPro domains: - IPR036157: dUTPase-like superfamily (homologous_superfamily) [54-204]
- IPR033704: dUTPase, trimeric (domain) [87-174]
- IPR029054: dUTPase-like (domain) [75-203]
- IPR008181: Deoxyuridine triphosphate nucleotidohydrolase (family) [55-204]
Interaction partners: - Thymidylate synthase
- Thymidine kinase, cytosolic
- Inosine triphosphate pyrophosphatase
- Deoxycytidylate deaminase
- dTMP kinase
- Nucleoside diphosphate kinase homolog 7
- Adenylate kinase 9
- Nucleoside diphosphate kinase A
- Nucleoside diphosphate kinase 6
- Nucleoside diphosphate kinase B
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0043169 (cation binding), GO:0032553 (ribonucleotide binding), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0032555 (purine ribonucleotide binding), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0046872 (metal ion binding), GO:0017076 (purine nucleotide binding), GO:0016462 (pyrophosphatase activity), GO:0000287 (magnesium ion binding), GO:0047429 (nucleoside triphosphate diphosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0006793 (phosphorus metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0019693 (ribose phosphate metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0090407 (organophosphate biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0072527 (pyrimidine-containing compound metabolic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0009259 (ribonucleotide metabolic process), GO:0009141 (nucleoside triphosphate metabolic process), GO:0072528 (pyrimidine-containing compound biosynthetic process), GO:0006220 (pyrimidine nucleotide metabolic process), GO:0009117 (nucleotide metabolic process), GO:0046390 (ribose phosphate biosynthetic process), GO:1901293 (nucleoside phosphate biosynthetic process), GO:0009129 (pyrimidine nucleoside monophosphate metabolic process), GO:0009124 (nucleoside monophosphate biosynthetic process), GO:0009165 (nucleotide biosynthetic process), GO:0009260 (ribonucleotide biosynthetic process), GO:0006221 (pyrimidine nucleotide biosynthetic process), GO:0009199 (ribonucleoside triphosphate metabolic process), GO:0009147 (pyrimidine nucleoside triphosphate metabolic process), GO:0006221 (pyrimidine nucleotide biosynthetic process), GO:0009142 (nucleoside triphosphate biosynthetic process), GO:0009167 (purine ribonucleoside monophosphate metabolic process), GO:0009145 (purine nucleoside triphosphate biosynthetic process), GO:0009220 (pyrimidine ribonucleotide biosynthetic process), GO:0009201 (ribonucleoside triphosphate biosynthetic process), GO:0046034 (ATP metabolic process), GO:0009205 (purine ribonucleoside triphosphate metabolic process), GO:0009152 (purine ribonucleotide biosynthetic process), GO:0006754 (ATP biosynthetic process), GO:0009206 (purine ribonucleoside triphosphate biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)