Protein: Diacylglycerol kinase epsilon (DAG kinase epsilon) (EC 2.7.1.107) (Diglyceride kinase epsilon) (DGK-epsilon) (P52429)
Organism: Homo sapiens (Human)
Function: Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle. Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently.
Subcellular location: Membrane {ECO:0000269|PubMed:23542698}; Single-pass membrane protein {ECO:0000255}. Cytoplasm {ECO:0000269|PubMed:23542698}.
Sequence: MEAERRPAPGSPSEGLFADGHLILWTLCSVLLPVFITFWCSLQRSRRQLHRRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLRKADKRFQCKEIMLKNDTKVLDAMPHHWIRGNVPLCSYCMVCKQQCGCQPKLCDYRCIWCQKTVHDECMKNSLKNEKCDFGEFKNLIIPPSYLTSINQMRKDKKTDYEVLASKLGKQWTPLIILANSRSGTNMGEGLLGEFRILLNPVQVFDVTKTPPIKALQLCTLLPYYSARVLVCGGDGTVGWVLDAVDDMKIKGQEKYIPQVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVALPSLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQIQVKLANPFRIGQAHTVRLILKCSMMPMQVDGEPWAQGPCTVTITHKTHAMMLYFSGEQTDDDISSTSDQEDIKATE
InterPro domains: - IPR046349: C1-like domain superfamily (homologous_superfamily) [60-180]
- IPR001206: Diacylglycerol kinase, catalytic domain (domain) [215-356]
- IPR002219: Protein kinase C-like, phorbol ester/diacylglycerol-binding domain (domain) [58-177]
- IPR000756: Diacylglycerol kinase, accessory domain (domain) [369-524]
- IPR017438: Inorganic polyphosphate/ATP-NAD kinase, N-terminal (homologous_superfamily) [218-353]
- IPR016064: NAD kinase/diacylglycerol kinase-like domain superfamily (homologous_superfamily) [219-542]
- IPR037607: Diacylglycerol kinase (family) [53-544]
Interaction partners: - Lipoprotein lipase
- Dihydroxyacetone phosphate acyltransferase
- Glycerol-3-phosphate dehydrogenase, mitochondrial
- Thrombomodulin
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
- Complement factor B
- Complement factor H-related protein 5
- Complement factor H-related protein 1
- Cyanocobalamin reductase / alkylcobalamin dealkylase
- Complement factor H
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0016301 (kinase activity), GO:0004143 (diacylglycerol kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032501 (multicellular organismal process), GO:0050817 (coagulation), GO:0065008 (regulation of biological quality), GO:0001775 (cell activation), GO:0006950 (response to stress), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0030168 (platelet activation), GO:0050878 (regulation of body fluid levels), GO:0007596 (blood coagulation), GO:0006629 (lipid metabolic process), GO:0009611 (response to wounding), GO:0006793 (phosphorus metabolic process), GO:0030258 (lipid modification), GO:0042060 (wound healing), GO:0006796 (phosphate-containing compound metabolic process), GO:0046486 (glycerolipid metabolic process), GO:0007599 (hemostasis), GO:0046834 (lipid phosphorylation), GO:0016310 (phosphorylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)