Protein: DASH complex subunit DAD2 (DUO1 and DAM1-interacting protein 2) (Helper of ASK1 protein 1) (Outer kinetochore protein DAD2) (P36162)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids.
Subcellular location: Nucleus {ECO:0000269|PubMed:14562095}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:11782438, ECO:0000269|PubMed:14562095}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:11782438, ECO:0000269|PubMed:14562095}. Chromosome {ECO:0000269|PubMed:36701236}. Note=Associates with the mitotic spindle and the kinetochore (PubMed:14562095). Associates with origin of replication (ORI) sites during interphase (PubMed:36701236). {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:36701236}.
Sequence: MDSIDEQIAIKRKELQSLQKITSLTDGLKIQLTELNEQIKEMGMNADSVAQLMNNWDSIINNISQASLGLLQYAEGDYEIGPWKDSKKKESEQSNETGLEAQENDKNDEDNDEDEDLVPLPETMVRIRVDGNE
InterPro domains: - IPR013963: DASH complex subunit Dad2 (family) [2-133]
Interaction partners: - DASH complex subunit ASK1
- DASH complex subunit SPC34
- DASH complex subunit DUO1
- DASH complex subunit DAD4
- DASH complex subunit DAM1
- DASH complex subunit DAD3
- DASH complex subunit DAD1
- DASH complex subunit SPC19
- DASH complex subunit HSK3
- Inner kinetochore subunit CHL4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0008092 (cytoskeletal protein binding), GO:0015631 (tubulin binding), GO:0008017 (microtubule binding), GO:0051010 (microtubule plus-end binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0022402 (cell cycle process), GO:0050794 (regulation of cellular process), GO:0007049 (cell cycle), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0051640 (organelle localization), GO:0048522 (positive regulation of cellular process), GO:0051726 (regulation of cell cycle), GO:0090068 (positive regulation of cell cycle process), GO:0007059 (chromosome segregation), GO:1903047 (mitotic cell cycle process), GO:0051656 (establishment of organelle localization), GO:0050000 (chromosome localization), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0051130 (positive regulation of cellular component organization), GO:0008608 (attachment of spindle microtubules to kinetochore), GO:0032886 (regulation of microtubule-based process), GO:0051128 (regulation of cellular component organization), GO:0045787 (positive regulation of cell cycle), GO:0044089 (positive regulation of cellular component biogenesis), GO:0010564 (regulation of cell cycle process), GO:0000278 (mitotic cell cycle), GO:0044087 (regulation of cellular component biogenesis), GO:0051315 (attachment of mitotic spindle microtubules to kinetochore), GO:1902903 (regulation of supramolecular fiber organization), GO:0051984 (positive regulation of chromosome segregation), GO:0051983 (regulation of chromosome segregation), GO:0070507 (regulation of microtubule cytoskeleton organization), GO:0043254 (regulation of protein-containing complex assembly), GO:0098813 (nuclear chromosome segregation), GO:1902905 (positive regulation of supramolecular fiber organization), GO:0051988 (regulation of attachment of spindle microtubules to kinetochore), GO:0140014 (mitotic nuclear division), GO:0051987 (positive regulation of attachment of spindle microtubules to kinetochore), GO:0007080 (mitotic metaphase plate congression), GO:0031134 (sister chromatid biorientation), GO:0033043 (regulation of organelle organization), GO:0010638 (positive regulation of organelle organization), GO:0006996 (organelle organization), GO:0000070 (mitotic sister chromatid segregation), GO:0051303 (establishment of chromosome localization), GO:0031334 (positive regulation of protein-containing complex assembly), GO:0051493 (regulation of cytoskeleton organization), GO:0031116 (positive regulation of microtubule polymerization), GO:0051310 (metaphase plate congression), GO:0000819 (sister chromatid segregation), GO:0031110 (regulation of microtubule polymerization or depolymerization), GO:0032271 (regulation of protein polymerization), GO:1990758 (mitotic sister chromatid biorientation), GO:0031112 (positive regulation of microtubule polymerization or depolymerization), GO:0031113 (regulation of microtubule polymerization), GO:0048285 (organelle fission), GO:0032273 (positive regulation of protein polymerization), GO:0051276 (chromosome organization), GO:0051495 (positive regulation of cytoskeleton organization), GO:0000280 (nuclear division)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098687 (chromosomal region), GO:0000940 (outer kinetochore), GO:0140513 (nuclear protein-containing complex), GO:0099080 (supramolecular complex), GO:0042729 (DASH complex), GO:0000776 (kinetochore), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000775 (chromosome, centromeric region), GO:0043228 (non-membrane-bounded organelle), GO:0000779 (condensed chromosome, centromeric region), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome), GO:0005634 (nucleus), GO:0000793 (condensed chromosome)