Protein: Histidine--tRNA ligase, mitochondrial (EC 6.1.1.21) (Histidine--tRNA ligase-like) (Histidyl-tRNA synthetase) (HisRS) (Q99KK9)
Organism: Mus musculus (Mouse)
Function: Mitochondrial aminoacyl-tRNA synthetase that catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP).
Subcellular location: Mitochondrion {ECO:0000250|UniProtKB:P49590}.
Sequence: MPHLGPLRRRAWAALLGQLLRPPSTVCTRGCHSQVAKAVLTSEQLKSHQEKPNFVIKVPKGTRDLSPQQMVVREKILDKIISCFKRHGAKGLDTPAFELKEMLTEKYEDNFGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKLKKMKRYQVGKVWRRESPAIAQGRYREFCQCDFDIAGEFDPMIPDAECLRIMCEILSGLQLGDFLIKVNDRRVVDGIFAVCGVPESKLRTICSSMDKLDKMSWEGVRHEMVAKKGLAPEVADRIGDFVQYHGGISLVEDLFKDPRLSQSQLALQGLGDLKLLFEYLRLFGIADKISLDLSLARGLDYYTGVIYEAVLLESPAQAGKETLSVGSVAAGGRYDNLVAQFDPKGHHVPCVGLSIGVERIFYLVEQKMKMSGEKVRTTETQVFVATPQKNFLQERLKIIAELWDAGIKAEMLYKNNPKLLTQLHYCEKADIPLMVIIGEQERNEGVIKLRSVASREEVTINRESLVAEIQKRLSES
InterPro domains: - IPR036621: Anticodon-binding domain superfamily (homologous_superfamily) [405-505]
- IPR045864: Class II Aminoacyl-tRNA synthetase/Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL) (homologous_superfamily) [44-404]
- IPR041715: Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain (domain) [61-394]
- IPR004516: Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunit (family) [53-502]
- IPR004154: Anticodon-binding (domain) [410-501]
- IPR033656: Histidyl-anticodon-binding (domain) [408-499]
- IPR006195: Aminoacyl-tRNA synthetase, class II (domain) [61-416]
- IPR015807: Histidine-tRNA ligase (family) [58-489]
Interaction partners: - Histidine--tRNA ligase, cytoplasmic
- Tyrosine--tRNA ligase, cytoplasmic
- Asparaginyl-tRNA synthetase
- Heme oxygenase 2
- Lysine--tRNA ligase
- Bifunctional glutamate/proline--tRNA ligase
- Aspartate--tRNA ligase, mitochondrial
- Methionine--tRNA ligase, cytoplasmic
- Alanine--tRNA ligase, cytoplasmic
- Tryptophan--tRNA ligase, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0140098 (catalytic activity, acting on RNA), GO:0016875 (ligase activity, forming carbon-oxygen bonds), GO:0004812 (aminoacyl-tRNA ligase activity), GO:0140101 (catalytic activity, acting on a tRNA)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0006082 (organic acid metabolic process), GO:0043603 (amide metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0006518 (peptide metabolic process), GO:0043436 (oxoacid metabolic process), GO:0043604 (amide biosynthetic process), GO:0043038 (amino acid activation), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006412 (translation), GO:0090304 (nucleic acid metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0043043 (peptide biosynthetic process), GO:0043039 (tRNA aminoacylation), GO:0019752 (carboxylic acid metabolic process), GO:0006418 (tRNA aminoacylation for protein translation), GO:0034660 (ncRNA metabolic process), GO:0006399 (tRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)