Protein: Histone acetyltransferase GCN5 (AtGCN5) (EC 2.3.1.48) (BIG TOP protein) (Q9AR19)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Acetylates histone H3 and ADA2 proteins in vitro. Acetylates 'Lys-14' of histone H3. Acetylation of histones gives a specific tag for epigenetic transcription activation. Operates in concert with certain DNA-binding transcriptional activators. Acts via the formation of large multiprotein complexes that modify the chromatin (By similarity).
Subcellular location: Nucleus {ECO:0000305}.
Sequence: MDSHSSHLNAANRSRSSQTPSPSHSASASVTSSLHKRKLAATTAANAAASEDHAPPSSSFPPSSFSADTRDGALTSNDELESISARGADTDSDPDESEDIVVDDDEDEFAPEQDQDSSIRTFTAARLDSSSGVNGSSRNTKLKTESSTVKLESSDGGKDGGSSVVGTGVSGTVGGSSISGLVPKDESVKVLAENFQTSGAYIAREEALKREEQAGRLKFVCYSNDSIDEHMMCLIGLKNIFARQLPNMPKEYIVRLLMDRKHKSVMVLRGNLVVGGITYRPYHSQKFGEIAFCAITADEQVKGYGTRLMNHLKQHARDVDGLTHFLTYADNNAVGYFVKQGFTKEIYLEKDVWHGFIKDYDGGLLMECKIDPKLPYTDLSSMIRQQRKAIDERIRELSNCQNVYPKIEFLKNEAGIPRKIIKVEEIRGLREAGWTPDQWGHTRFKLFNGSADMVTNQKQLNALMRALLKTMQDHADAWPFKEPVDSRDVPDYYDIIKDPIDLKVIAKRVESEQYYVTLDMFVADARRMFNNCRTYNSPDTIYYKCATRLETHFHSKVQAGLQSGAKSQ
InterPro domains: - IPR000182: GNAT domain (domain) [222-371]
- IPR018359: Bromodomain, conserved site (conserved_site) [477-535]
- IPR037800: Histone acetyltransferase GCN5 (family) [10-564]
- IPR016181: Acyl-CoA N-acyltransferase (homologous_superfamily) [227-376]
- IPR036427: Bromodomain-like superfamily (homologous_superfamily) [449-565]
- IPR001487: Bromodomain (domain) [453-562]
Interaction partners: - Transcriptional adapter ADA2b
- Transcriptional adapter ADA2a
- Transcription initiation factor TFIID subunit 9
- Histone H4
- Transcription initiation factor TFIID subunit 10
- Ubiquitin C-terminal hydrolase 22
- Transcription initiation factor TFIID subunit 5
- Histone H3.3
- Transcription initiation factor TFIID subunit 6b
- Transcription and mRNA export factor ENY2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0061733 (peptide-lysine-N-acetyltransferase activity), GO:0016746 (acyltransferase activity), GO:0004402 (histone acetyltransferase activity), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups), GO:0016407 (acetyltransferase activity), GO:0016410 (N-acyltransferase activity), GO:0010484 (histone H3 acetyltransferase activity), GO:0010485 (histone H4 acetyltransferase activity), GO:0008080 (N-acetyltransferase activity), GO:0034212 (peptide N-acetyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009892 (negative regulation of metabolic process), GO:0009719 (response to endogenous stimulus), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0051716 (cellular response to stimulus), GO:0048523 (negative regulation of cellular process), GO:0044238 (primary metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0019538 (protein metabolic process), GO:0071495 (cellular response to endogenous stimulus), GO:0060255 (regulation of macromolecule metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:0009891 (positive regulation of biosynthetic process), GO:0009755 (hormone-mediated signaling pathway), GO:0031325 (positive regulation of cellular metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0071310 (cellular response to organic substance), GO:0010468 (regulation of gene expression), GO:0036211 (protein modification process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0032870 (cellular response to hormone stimulus), GO:0043412 (macromolecule modification), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0051253 (negative regulation of RNA metabolic process), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0031328 (positive regulation of cellular biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0043543 (protein acylation), GO:0018193 (peptidyl-amino acid modification), GO:1902680 (positive regulation of RNA biosynthetic process), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0016570 (histone modification), GO:0045893 (positive regulation of DNA-templated transcription), GO:0045892 (negative regulation of DNA-templated transcription), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:0016573 (histone acetylation), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0006473 (protein acetylation), GO:0018205 (peptidyl-lysine modification), GO:0043967 (histone H4 acetylation), GO:0018394 (peptidyl-lysine acetylation), GO:0006475 (internal protein amino acid acetylation), GO:0018393 (internal peptidyl-lysine acetylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005829 (cytosol), GO:1902494 (catalytic complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0140535 (intracellular protein-containing complex), GO:1905368 (peptidase complex), GO:0043229 (intracellular organelle), GO:0031248 (protein acetyltransferase complex), GO:1990234 (transferase complex), GO:0043227 (membrane-bounded organelle), GO:0070461 (SAGA-type complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)