Protein: Dehydrodolichyl diphosphate synthase complex subunit NUS1 (EC 2.5.1.87) (Cis-prenyltransferase subunit NgBR) (Nogo-B receptor) (NgBR) (Nuclear undecaprenyl pyrophosphate synthase 1 homolog) (Q96E22)
Organism: Homo sapiens (Human)
Function: With DHDDS, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Both subunits contribute to enzymatic activity, i.e. condensation of multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol phosphate which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER). Synthesizes long-chain polyprenols, mostly of C95 and C100 chain length. Regulates the glycosylation and stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol. Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:19723497, ECO:0000269|PubMed:21572394}; Multi-pass membrane protein {ECO:0000303|PubMed:21572394}. Note=Colocalizes with Nogo-B during VEGF and wound healing angiogenesis. {ECO:0000269|PubMed:19723497}.
Sequence: MTGLYELVWRVLHALLCLHRTLTSWLRVRFGTWNWIWRRCCRAASAAVLAPLGFTLRKPPAVGRNRRHHRHPRGGSCLAAAHHRMRWRADGRSLEKLPVHMGLVITEVEQEPSFSDIASLVVWCMAVGISYISVYDHQGIFKRNNSRLMDEILKQQQELLGLDCSKYSPEFANSNDKDDQVLNCHLAVKVLSPEDGKADIVRAAQDFCQLVAQKQKRPTDLDVDTLASLLSSNGCPDPDLVLKFGPVDSTLGFLPWHIRLTEIVSLPSHLNISYEDFFSALRQYAACEQRLGK
InterPro domains: - IPR038887: Dehydrodolichyl diphosphate synthase complex subunit Nus1 (family) [3-293]
- IPR001441: Decaprenyl diphosphate synthase-like (family) [186-292]
- IPR036424: Decaprenyl diphosphate synthase-like superfamily (homologous_superfamily) [93-293]
Interaction partners: - Dehydrodolichyl diphosphate synthase complex subunit DHDDS
- Geranylgeranyl pyrophosphate synthase
- Farnesyl pyrophosphate synthase
- All trans-polyprenyl-diphosphate synthase PDSS1
- All trans-polyprenyl-diphosphate synthase PDSS2
- Reticulon-4
- Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha
- Protein farnesyltransferase subunit beta
- 4-hydroxybenzoate polyprenyltransferase, mitochondrial
- Squalene synthase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016765 (transferase activity, transferring alkyl or aryl (other than methyl) groups), GO:0004659 (prenyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0070085 (glycosylation), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0065009 (regulation of molecular function), GO:0044238 (primary metabolic process), GO:0050790 (regulation of catalytic activity), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0019538 (protein metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0043170 (macromolecule metabolic process), GO:0043413 (macromolecule glycosylation), GO:1901135 (carbohydrate derivative metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0051336 (regulation of hydrolase activity), GO:1903018 (regulation of glycoprotein metabolic process), GO:0036211 (protein modification process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006486 (protein glycosylation), GO:0043412 (macromolecule modification), GO:0009100 (glycoprotein metabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0051346 (negative regulation of hydrolase activity), GO:0031399 (regulation of protein modification process), GO:2000112 (regulation of cellular macromolecule biosynthetic process), GO:0060049 (regulation of protein glycosylation), GO:0031400 (negative regulation of protein modification process), GO:0009101 (glycoprotein biosynthetic process), GO:0010559 (regulation of glycoprotein biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0016020 (membrane)