Protein: Acetyl-CoA carboxylase (EC 6.4.1.2) (ACC-alpha) [Includes: Biotin carboxylase (EC 6.3.4.14)] (Q54J08)
Organism: Dictyostelium discoideum (Social amoeba)
Function: Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
Subcellular location: Cytoplasm {ECO:0000250}.
Sequence: MIEINEYIKKLGGDKNIEKILIANNGIAAVKAIRSVRKWAYTNFGNERAIKFVVMATPEDMKANAEYIRMADQILQVPGGSNNNNYANVDIIVDFAERAGVQAVWAGWGHASENPRLPDLLSKTETGIVFIGPPAKAMADLGDKIASTIVAQSARVACVPWSGSGLKVDYSECNGVPSEIYGRACINSVEEARECAQRVGFPAMIKASEGGGGKGIRKVTSMEDLESSFRQVQNEVPGSPIFFMKLVSNARHLEVQIVADRHGEAISLNGRDCSVQRRHQKIIEEGPAIAPTPQVWEEMERAAVRLVKEVGYVGAGTVEYLFAEGEYYFLELNPRLQVEHPVTEQITGVNLPATQLQIAMGIPLHRIPDIRKLYGRTGDDLYGDSMIDLHDFTKRNPPAGHCIAVRITGENPDEGFKPTSGQIHELTFRSTPNIWGYFSVGAKGGLHEYADSQFGHIFANGATREEARKTIILGLKEISIRGDIRTPVEYIIHLLESKDFKENHIHTGWLDQLISEKIQTKKPETMIVVLCGAIYKASTIFSTKIQEFSNQLSNGQLPSLELLNNSLPIELIYNNVKYQFEVSRTGINNYSVCLKNDKSAVSIDSSVIMLSDSGLLILLNGSTHVCYGREDVIGLSLMIDSKTCVFSQEYDPSILRTSSPGKLVRYLVDDGSLVVKGTPFAEIEVMKMYMPLLVPEKGIIKFVLTEGSVMAPGAIIANLELSDQSSIQKSTVFTGSLTKMSPPTLIGNKPHQLLNYTLGKISNVLCGYESNDLNQLLNDTIKQLSNQKLPLFEFKEQLSIVQSRIPQSLFKLINDELNKFEFNNDDDDEDDSELFNSKNLQLSISLYLNKLLLENEQLSIAIQLLIKPIILLAEKYNDGVSFAAINIFKNYLEEFIQIETNLQNKNIQTVLKSIRPTYKDNISKVVDIAQSLHPQSKKYKFILLLLNKIQEQGLVCDFVEQFKKLSSLGGNCMEISLKAKHIMVHSQLPSNKQRSNDLINSLKSILNVNNEQQQQVDEKVQDNKDEKISKLSKQTNEISDILIPMFFNESNNDDDIRKLAMEVYVRHSYRSYYVEDTKVTLSNDEGSSGFSFIEWHFYINLPQQSNLGGSNSGSPTYGSPLIRSISSSGGSSGGSGFQISPRPSMSIFNGLSMLRTDSTDSLTAMEDQTKLRYGMMVFFENEKKFEEKLPLILTRYNEENNKKSQLSLSPNESTDILSVIISIYPESISQENQAISSFQSILKGYIKELSIARIRRITFICCGGDEGKPLKYFTFRERHMYMEDPIFRHIEPAMAYHLEVRKLSNFDITHVPTTSQRIHLYYAQEKGKKETDPDADRSFFVRSVIRYSDLYGHSNEIKVDILLSQIETLLSESIESLEIAMSNKKYEKAQNHSIFLNVMPEVMFDEKMIGYVVQEIGDRLGKRLWKLRVGRVEVRGRIRKGDGLIPVRFFVQNPTGYAFQVQCYYEQQNSIGQMVFAVVPGSSKGSWEGLPVDTPYPIMDAVQRNRFKAQRLDTTYCYDYPDLFREAMQNIWMEFMESNKTNPVKVYPSSRGVLESVELILPSTINTDFPPSIPLDQLPEESKPKLEETYRPIGYNDIGMVAWRMTFYTPEYPLGRQAIVIANDITHQIGSFGPQEDMLFKLASELARKEKIPRIYLSSNSGARIGLADEIKSKFKVTWNVPSDPTKGIKNLYLTNNDYQALKDSVIAYQDTTDKDKWIIHDIIGQKNGIGVENLSWSGLIAGETSQAYNEIFTITLVSGRSVGIGAYLVRLGQRTIQNDAPIILTGASALNKVLGKEVYESNQQLGGSQIMYPNGVSHIIVNDELRGITNVLQWLSFVPKSGGEMVPIISPIDSPHRDIEFDPSNSINGKCDTRHLIAGLQSELDPNYWISGMFDKDSFMETLAGWANTVITGRARLGGIPVGIIAVETKSVEKIIPADPANPLSYEQVNTQAGQVWYPDSSFKTAQAIADFNNGEELPLMILANWRGFSGGMRDMFDEILKFGSMIVDNLRNYKQPVMVYIPPFAELRGGAWVVLDSTINLDMMEMYCSEEGRGGVLEPNGIAEIKYRDPELIKTMHRLDPKLIEWDKSIPIGVSVNGLDQSQKTIKSQIQQREKELLGIYQQIAIKFADLHDTPGRMKAKGVIKQMVPWKSARSFFYDRIKRRLFEEDKLKLIDKSHPGLNRQSKLNLLETWIKQILGNNQSVDYHQNDKLISSTIESNSHIINDKIIDLSKQYAINQILNFVQSDSESIVDGFQNLLPFISTQQKEFLFESLKKDLNK
InterPro domains: - IPR005481: Biotin carboxylase-like, N-terminal domain (domain) [17-138]
- IPR016185: Pre-ATP-grasp domain superfamily (homologous_superfamily) [5-140]
- IPR000089: Biotin/lipoyl attachment (domain) [646-720]
- IPR005482: Biotin carboxylase, C-terminal (domain) [404-512]
- IPR011762: Acetyl-coenzyme A carboxyltransferase, N-terminal (domain) [1495-1851]
- IPR011761: ATP-grasp fold (domain) [170-360]
- IPR029045: ClpP/crotonase-like domain superfamily (homologous_superfamily) [1500-2246]
- IPR005479: Carbamoyl phosphate synthase, ATP-binding domain (domain) [183-365]
- IPR034733: Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (domain) [1610-2166]
- IPR011764: Biotin carboxylation domain (domain) [16-515]
- IPR001882: Biotin-binding site (binding_site) [677-694]
- IPR011054: Rudiment single hybrid motif (homologous_superfamily) [399-515]
- IPR013815: ATP-grasp fold, subdomain 1 (homologous_superfamily) [185-247]
- IPR049074: Acetyl-CoA carboxylase, BT domain (domain) [525-646]
- IPR013537: Acetyl-CoA carboxylase, central domain (domain) [720-1485]
- IPR049076: Acetyl-CoA carboxylase (family) [3-2278]
- IPR011763: Acetyl-coenzyme A carboxyltransferase, C-terminal (domain) [1852-2178]
- IPR011053: Single hybrid motif (homologous_superfamily) [649-721]
Interaction partners: - [acyl-carrier-protein] S-malonyltransferase
- Probable acetyl-CoA acetyltransferase
- malate synthase
- Malonyl-CoA decarboxylase C-terminal domain-containing protein
- Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
- Acetyl-coenzyme A synthetase
- medium-chain acyl-CoA ligase
- medium-chain acyl-CoA ligase
- Acyl-CoA synthetase family member 3
- Probable ATP-citrate synthase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:1901681 (sulfur compound binding), GO:0097159 (organic cyclic compound binding), GO:0033218 (amide binding), GO:0043167 (ion binding), GO:0019842 (vitamin binding), GO:0043177 (organic acid binding), GO:0016885 (ligase activity, forming carbon-carbon bonds), GO:0043168 (anion binding), GO:0031406 (carboxylic acid binding), GO:0016421 (CoA carboxylase activity), GO:0033293 (monocarboxylic acid binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006629 (lipid metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0035383 (thioester metabolic process), GO:0006793 (phosphorus metabolic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0006637 (acyl-CoA metabolic process), GO:0006644 (phospholipid metabolic process), GO:0019693 (ribose phosphate metabolic process), GO:0090407 (organophosphate biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:0008610 (lipid biosynthetic process), GO:0009259 (ribonucleotide metabolic process), GO:0008654 (phospholipid biosynthetic process), GO:0009117 (nucleotide metabolic process), GO:0006163 (purine nucleotide metabolic process), GO:0034032 (purine nucleoside bisphosphate metabolic process), GO:0006084 (acetyl-CoA metabolic process), GO:0033865 (nucleoside bisphosphate metabolic process), GO:0009150 (purine ribonucleotide metabolic process), GO:0033875 (ribonucleoside bisphosphate metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005811 (lipid droplet)