Protein: EH domain-containing protein 3 (PAST homolog 3) (Q9NZN3)
Organism: Homo sapiens (Human)
Function: ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes tubulation of endocytic membranes. Binding to phosphatidic acid induces its membrane tubulation activity (By similarity). Plays a role in endocytic transport. Involved in early endosome to recycling endosome compartment (ERC), retrograde early endosome to Golgi, and endosome to plasma membrane (rapid recycling) protein transport. Involved in the regulation of Golgi maintenance and morphology. Involved in the recycling of internalized D1 dopamine receptor. Plays a role in cardiac protein trafficking probably implicating ANK2. Involved in the ventricular membrane targeting of SLC8A1 and CACNA1C and probably the atrial membrane localization of CACNA1GG and CACNA1H implicated in the regulation of atrial myocyte excitability and cardiac conduction (By similarity). In conjunction with EHD4 may be involved in endocytic trafficking of KDR/VEGFR2 implicated in control of glomerular function (By similarity). Involved in the rapid recycling of integrin beta-3 implicated in cell adhesion maintenance. Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing (By similarity). Plays a role in the formation of the ciliary vesicle, an early step in cilium biogenesis; possibly sharing redundant functions with EHD1.
Subcellular location: Recycling endosome membrane {ECO:0000269|PubMed:17233914}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Cell membrane {ECO:0000269|PubMed:17233914}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Cell projection, cilium membrane {ECO:0000269|PubMed:25686250}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=Localizes to the ciliary pocket from where the cilium protrudes (PubMed:25686250). Colocalizes with RAB8A and MYO5B to a cytoplasmic tubular network devoid of RAB11A (By similarity). Colocalizes with ANK2 in myocyte perinuclear region (PubMed:20489164). Colocalizes with BACE1 in tubulovesicular cytoplasmic membranes. Colocalizes with BACE1 and APP amyloid beta proteins in hippocampal mossy fiber terminals (By similarity). {ECO:0000250|UniProtKB:Q9QXY6, ECO:0000269|PubMed:20489164, ECO:0000269|PubMed:25686250}.
Sequence: MFSWLGTDDRRRKDPEVFQTVSEGLKKLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMQGDMEGIIPGNALVVDPKKPFRKLNAFGNAFLNRFVCAQLPNPVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKMRVVLNKADQIETQQLMRVYGALMWSLGKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPSVFGKDNKKKELVNNLAEIYGRIEREHQISPGDFPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPIQMVKGGAFEGTLHGPFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSVLGKIWKLADIDKDGMLDDDEFALANHLIKVKLEGHELPNELPAHLLPPSKRKVAE
InterPro domains: - IPR040990: Domain of unknown function DUF5600 (domain) [288-394]
- IPR000261: EH domain (domain) [438-533]
- IPR030381: Dynamin-type guanine nucleotide-binding (G) domain (domain) [55-286]
- IPR018247: EF-Hand 1, calcium-binding site (binding_site) [489-501]
- IPR011992: EF-hand domain pair (homologous_superfamily) [443-531]
- IPR002048: EF-hand domain (domain) [476-511]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [53-306]
- IPR045063: Dynamin, N-terminal (domain) [61-221]
- IPR031692: EH domain-containing protein, N-terminal (domain) [24-56]
Interaction partners: - Rabenosyn-5
- EH domain-binding protein 1
- MICAL-like protein 1
- Rab11 family-interacting protein 2
- Protein kinase C and casein kinase substrate in neurons protein 2
- EH domain-containing protein 1
- Dysferlin
- Caveolae-associated protein 1
- Myoferlin
- Ras-related protein Rab-5A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0050839 (cell adhesion molecule binding), GO:0045296 (cadherin binding)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0033036 (macromolecule localization), GO:0044085 (cellular component biogenesis), GO:0046907 (intracellular transport), GO:0006810 (transport), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0051668 (localization within membrane), GO:0045184 (establishment of protein localization), GO:0006886 (intracellular protein transport), GO:0030030 (cell projection organization), GO:0071705 (nitrogen compound transport), GO:0016197 (endosomal transport), GO:0098876 (vesicle-mediated transport to the plasma membrane), GO:0015031 (protein transport), GO:0071702 (organic substance transport), GO:0016192 (vesicle-mediated transport), GO:0043933 (protein-containing complex organization), GO:0008104 (protein localization), GO:0006996 (organelle organization), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0033365 (protein localization to organelle), GO:0006897 (endocytosis), GO:0065003 (protein-containing complex assembly), GO:0030031 (cell projection assembly), GO:0032456 (endocytic recycling), GO:0120036 (plasma membrane bounded cell projection organization), GO:0044782 (cilium organization), GO:0061512 (protein localization to cilium), GO:0051259 (protein complex oligomerization), GO:0120031 (plasma membrane bounded cell projection assembly), GO:0060271 (cilium assembly), GO:0051260 (protein homooligomerization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0020016 (ciliary pocket), GO:0071944 (cell periphery), GO:0005615 (extracellular space), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005576 (extracellular region), GO:0031090 (organelle membrane), GO:0120025 (plasma membrane bounded cell projection), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043230 (extracellular organelle), GO:0098590 (plasma membrane region), GO:0070062 (extracellular exosome), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0055037 (recycling endosome), GO:0031253 (cell projection membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0005929 (cilium), GO:0098588 (bounding membrane of organelle), GO:0012506 (vesicle membrane), GO:0065010 (extracellular membrane-bounded organelle), GO:0005769 (early endosome), GO:0010008 (endosome membrane), GO:0031982 (vesicle), GO:0030659 (cytoplasmic vesicle membrane), GO:0060170 (ciliary membrane), GO:1903561 (extracellular vesicle), GO:0097708 (intracellular vesicle), GO:0031901 (early endosome membrane), GO:0055038 (recycling endosome membrane)