Protein: Ras-related protein Ral-A (EC 3.6.5.2) (P63321)
Organism: Mus musculus (Mouse)
Function: Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells (By similarity). The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. During mitosis, supports the stabilization and elongation of the intracellular bridge between dividing cells. Cooperates with EXOC2 to recruit other components of the exocyst to the early midbody (By similarity). During mitosis, also controls mitochondrial fission by recruiting to the mitochondrion RALBP1, which mediates the phosphorylation and activation of DNM1L by the mitotic kinase cyclin B-CDK1 (By similarity).
Subcellular location: Cell membrane {ECO:0000250|UniProtKB:P11233}; Lipid-anchor {ECO:0000250|UniProtKB:P11233}; Cytoplasmic side {ECO:0000250|UniProtKB:P11233}. Cleavage furrow {ECO:0000250|UniProtKB:P11233}. Midbody, Midbody ring {ECO:0000250|UniProtKB:P11233}. Mitochondrion {ECO:0000250|UniProtKB:P11233}. Note=Predominantly at the cell surface in the absence of LPA. In the presence of LPA, colocalizes with LPAR1 and LPAR2 in endocytic vesicles. May colocalize with CNTRL/centriolin at the midbody ring. However, localization at the midbody at late cytokinesis was not confirmed. Relocalizes to the mitochondrion during mitosis where it regulates mitochondrial fission. {ECO:0000250|UniProtKB:P11233}.
Sequence: MAANKPKGQNSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITEMESFAATADFREQILRVKEDENVPFLLVGNKSDLEDKRQVSVEEAKNRADQWNVNYVETSAKTRANVDKVFFDLMREIRARKMEDSKEKNGKKKRKSLAKRIRERCCIL
InterPro domains: - IPR001806: Small GTPase (family) [10-206]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [8-190]
- IPR005225: Small GTP-binding domain (domain) [16-168]
- IPR020849: Small GTPase, Ras-type (family) [15-193]
Interaction partners: - RalA-binding protein 1
- Exocyst complex component 2
- Exocyst complex component 8
- Ral guanine nucleotide dissociation stimulator
- Exocyst complex component 4
- Phospholipase D1
- Ral guanine nucleotide dissociation stimulator-like 2
- Ral guanine nucleotide dissociation stimulator-like 1
- Phospholipase D2
- Exocyst complex component 5
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:0005515 (protein binding), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0019899 (enzyme binding), GO:0032553 (ribonucleotide binding), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0019900 (kinase binding), GO:0032555 (purine ribonucleotide binding), GO:0017076 (purine nucleotide binding), GO:0005525 (GTP binding), GO:0019001 (guanyl nucleotide binding), GO:0032561 (guanyl ribonucleotide binding), GO:0019901 (protein kinase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0065008 (regulation of biological quality), GO:0032879 (regulation of localization), GO:0050794 (regulation of cellular process), GO:0051128 (regulation of cellular component organization), GO:0060627 (regulation of vesicle-mediated transport), GO:0099149 (regulation of postsynaptic neurotransmitter receptor internalization), GO:0051049 (regulation of transport), GO:0030100 (regulation of endocytosis), GO:0048259 (regulation of receptor-mediated endocytosis), GO:0002090 (regulation of receptor internalization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0030054 (cell junction), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0005739 (mitochondrion), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0045202 (synapse), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0030139 (endocytic vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0097708 (intracellular vesicle)