Protein: Serine hydroxymethyltransferase 1 (SHM1) (SHMT 1) (Serine methylase 1) (EC 2.1.2.1) (P9WGI9)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. Thus, is able to catalyze the cleavage of L-allo-threonine.
Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00051, ECO:0000305|PubMed:12913008}.
Sequence: MTAAPDARTTAVMSAPLAEVDPDIAELLAKELGRQRDTLEMIASENFVPRAVLQAQGSVLTNKYAEGLPGRRYYGGCEHVDVVENLARDRAKALFGAEFANVQPHSGAQANAAVLHALMSPGERLLGLDLANGGHLTHGMRLNFSGKLYENGFYGVDPATHLIDMDAVRATALEFRPKVIIAGWSAYPRVLDFAAFRSIADEVGAKLLVDMAHFAGLVAAGLHPSPVPHADVVSTTVHKTLGGGRSGLIVGKQQYAKAINSAVFPGQQGGPLMHVIAGKAVALKIAATPEFADRQRRTLSGARIIADRLMAPDVAKAGVSVVSGGTDVHLVLVDLRDSPLDGQAAEDLLHEVGITVNRNAVPNDPRPPMVTSGLRIGTPALATRGFGDTEFTEVADIIATALATGSSVDVSALKDRATRLARAFPLYDGLEEWSLVGR
InterPro domains: - IPR019798: Serine hydroxymethyltransferase, pyridoxal phosphate binding site (binding_site) [231-247]
- IPR049943: Serine hydroxymethyltransferase-like (family) [11-404]
- IPR015421: Pyridoxal phosphate-dependent transferase, major domain (homologous_superfamily) [46-287]
- IPR039429: Serine hydroxymethyltransferase-like domain (domain) [17-398]
- IPR015424: Pyridoxal phosphate-dependent transferase (homologous_superfamily) [9-428]
- IPR015422: Pyridoxal phosphate-dependent transferase, small domain (homologous_superfamily) [20-427]
- IPR001085: Serine hydroxymethyltransferase (family) [2-428]
Interaction partners: - Probable glycine dehydrogenase
- Aminomethyltransferase
- Bifunctional purine biosynthesis protein PurH
- Bifunctional protein FolD
- Phosphoribosylglycinamide formyltransferase
- L-serine dehydratase
- Glycine cleavage system H protein
- Phosphoserine phosphatase SerB2
- L-threonine dehydratase biosynthetic IlvA
- Formyltetrahydrofolate deformylase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0016740 (transferase activity), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0019842 (vitamin binding), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0046983 (protein dimerization activity), GO:0043168 (anion binding), GO:0042802 (identical protein binding), GO:0070279 (vitamin B6 binding), GO:0016742 (hydroxymethyl-, formyl- and related transferase activity), GO:0042803 (protein homodimerization activity), GO:0030170 (pyridoxal phosphate binding), GO:0004372 (glycine hydroxymethyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006520 (amino acid metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006082 (organic acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0009069 (serine family amino acid metabolic process), GO:0006544 (glycine metabolic process), GO:0006563 (L-serine metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)