Protein: Formamidopyrimidine-DNA glycosylase (P05523)
Organism: Escherichia coli (strain K12)
Function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as a DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG) and its derivatives such as guanidinohydantoin:C and spiroiminodihydantoin:C, however it also acts on thymine glycol:G, 5,6-dihydrouracil:G and 5-hydroxyuracil:G. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Cleaves ssDNA containing an AP site
Sequence: MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRLSDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK
InterPro domains: - IPR015887: DNA glycosylase/AP lyase, zinc finger domain, DNA-binding site (binding_site) [244-268]
- IPR015886: Formamidopyrimidine-DNA glycosylase, H2TH DNA-binding (domain) [129-221]
- IPR010979: Small ribosomal subunit protein uS13-like, H2TH (homologous_superfamily) [130-222]
- IPR010663: Zinc finger, FPG/IleRS-type (domain) [241-269]
- IPR000214: Zinc finger, DNA glycosylase/AP lyase-type (domain) [235-269]
- IPR020629: Formamidopyrimidine-DNA glycosylase (family) [1-269]
- IPR035937: Formamidopyrimidine-DNA glycosylase, N-terminal (homologous_superfamily) [2-138]
- IPR012319: Formamidopyrimidine-DNA glycosylase, catalytic domain (domain) [1-115]
Interaction partners: - Endonuclease III
- Endonuclease III
- DNA polymerase I
- Adenine DNA glycosylase
- Dephospho-CoA kinase
- 8-oxo-dGTP diphosphatase
- Endonuclease 4
- Exodeoxyribonuclease III
- UvrABC system protein A
- DNA mismatch repair protein MutL
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0043167 (ion binding), GO:0016829 (lyase activity), GO:0043169 (cation binding), GO:0003676 (nucleic acid binding), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0016835 (carbon-oxygen lyase activity), GO:0016798 (hydrolase activity, acting on glycosyl bonds), GO:0140097 (catalytic activity, acting on DNA), GO:0016799 (hydrolase activity, hydrolyzing N-glycosyl compounds), GO:0004518 (nuclease activity), GO:0046872 (metal ion binding), GO:0003677 (DNA binding), GO:0003906 (DNA-(apurinic or apyrimidinic site) endonuclease activity), GO:0019104 (DNA N-glycosylase activity), GO:0003684 (damaged DNA binding), GO:0000702 (oxidized base lesion DNA N-glycosylase activity), GO:0046914 (transition metal ion binding), GO:0004519 (endonuclease activity), GO:0008270 (zinc ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0006259 (DNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0006281 (DNA repair), GO:0006284 (base-excision repair)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)