Protein: Diacylglycerol kinase zeta (DAG kinase zeta) (EC 2.7.1.107) (EC 2.7.1.93) (Diglyceride kinase zeta) (DGK-zeta) (Q13574)
Organism: Homo sapiens (Human)
Function: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group. The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol (By similarity). By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization. Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane (By similarity).
Subcellular location: Nucleus {ECO:0000269|PubMed:11257115, ECO:0000269|PubMed:15157668, ECO:0000269|PubMed:9159104, ECO:0000269|PubMed:9716136}. Cytoplasm, cytosol {ECO:0000269|PubMed:11257115, ECO:0000269|PubMed:22627129}. Cell membrane {ECO:0000269|PubMed:11257115}. Cell projection, lamellipodium {ECO:0000269|PubMed:15157668}.
Sequence: MEPRDGSPEARSSDSESASASSSGSERDAGPEPDKAPRRLNKRRFPGLRLFGHRKAITKSGLQHLAPPPPTPGAPCSESERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVARMLKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPKEALEMYRKVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEDRDEGATDRLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGMDLTPKIQDLKPQCVVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVVLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRIQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLQQEPDGAGAKSPTCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTPRSLQGDAAPPQGEELIEAAKRNDFCKLQELHRAGGDLMHRDEQSRTLLHHAVSTGSKDVVRYLLDHAPPEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV
InterPro domains: - IPR047484: Diacylglycerol kinase zeta, second protein kinase C conserved region 1 (domain) [170-244]
- IPR001206: Diacylglycerol kinase, catalytic domain (domain) [291-425]
- IPR047485: Diacylglycerol kinase zeta, first protein kinase C conserved region 1 (domain) [88-160]
- IPR036770: Ankyrin repeat-containing domain superfamily (homologous_superfamily) [785-928]
- IPR002219: Protein kinase C-like, phorbol ester/diacylglycerol-binding domain (domain) [96-230]
- IPR000756: Diacylglycerol kinase, accessory domain (domain) [446-603]
- IPR056383: Diacylglycerol kinase iota-like domain (domain) [632-755]
- IPR017438: Inorganic polyphosphate/ATP-NAD kinase, N-terminal (homologous_superfamily) [292-423]
- IPR002110: Ankyrin repeat (repeat) [794-889]
- IPR016064: NAD kinase/diacylglycerol kinase-like domain superfamily (homologous_superfamily) [292-622]
- IPR037607: Diacylglycerol kinase (family) [82-633]
Interaction partners: - Sorting nexin-27
- Lipoprotein lipase
- Myristoylated alanine-rich C-kinase substrate
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
- Dihydroxyacetone phosphate acyltransferase
- Disks large homolog 4
- Glycerol-3-phosphate dehydrogenase, mitochondrial
- Phospholipase D2
- Beta-arrestin-1
- Serine/threonine-protein phosphatase 6 regulatory subunit 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0016301 (kinase activity), GO:0004143 (diacylglycerol kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032501 (multicellular organismal process), GO:0050817 (coagulation), GO:0065008 (regulation of biological quality), GO:0001775 (cell activation), GO:0006950 (response to stress), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0030168 (platelet activation), GO:0050878 (regulation of body fluid levels), GO:0007596 (blood coagulation), GO:0006629 (lipid metabolic process), GO:0009611 (response to wounding), GO:0006793 (phosphorus metabolic process), GO:0030258 (lipid modification), GO:0042060 (wound healing), GO:0006796 (phosphate-containing compound metabolic process), GO:0007599 (hemostasis), GO:0046834 (lipid phosphorylation), GO:0016310 (phosphorylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0030054 (cell junction), GO:0045202 (synapse), GO:0098978 (glutamatergic synapse)