Protein: SH3 domain-containing kinase-binding protein 1 (CD2-binding protein 3) (CD2BP3) (Cbl-interacting protein of 85 kDa) (Human Src family kinase-binding protein 1) (HSB-1) (Q96B97)
Organism: Homo sapiens (Human)
Function: Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through an association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may be inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit (By similarity). May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. Has an essential role in the stimulation of B cell activation.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:20221403}. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein. Synapse, synaptosome. Cell junction, focal adhesion {ECO:0000250}. Note=Localized in endocytic vesicles containing clustered receptors. Colocalizes with ASAP1 in vesicular structures. Colocalized with actin microfilaments and focal adhesions (By similarity). Colocalized with MAGI2 in synaptosomes. Translocation to EGFR containing vesicles upon EGF stimulation is inhibited in the presence of SH3KBP1 (By similarity). Colocalizes with ZFP36 in the cytoplasm (PubMed:20221403). {ECO:0000250|UniProtKB:Q8R550, ECO:0000269|PubMed:20221403}.
Sequence: MVEAIVEFDYQAQHDDELTISVGEIITNIRKEDGGWWEGQINGRRGLFPDNFVREIKKEMKKDPLTNKAPEKPLHEVPSGNSLLSSETILRTNKRGERRRRRCQVAFSYLPQNDDELELKVGDIIEVVGEVEEGWWEGVLNGKTGMFPSNFIKELSGESDELGISQDEQLSKSSLRETTGSESDGGDSSSTKSEGANGTVATAAIQPKKVKGVGFGDIFKDKPIKLRPRSIEVENDFLPVEKTIGKKLPATTATPDSSKTEMDSRTKSKDYCKVIFPYEAQNDDELTIKEGDIVTLINKDCIDVGWWEGELNGRRGVFPDNFVKLLPPDFEKEGNRPKKPPPPSAPVIKQGAGTTERKHEIKKIPPERPEMLPNRTEEKERPEREPKLDLQKPSVPAIPPKKPRPPKTNSLSRPGALPPRRPERPVGPLTHTRGDSPKIDLAGSSLSGILDKDLSDRSNDIDLEGFDSVVSSTEKLSHPTTSRPKATGRRPPSQSLTSSSLSSPDIFDSPSPEEDKEEHISLAHRGVDASKKTSKTVTISQVSDNKASLPPKPGTMAAGGGGPAPLSSAAPSPLSSSLGTAGHRANSPSLFGTEGKPKMEPAASSQAAVEELRTQVRELRSIIETMKDQQKREIKQLLSELDEEKKIRLRLQMEVNDIKKALQSK
InterPro domains: - IPR036028: SH3-like domain superfamily (homologous_superfamily) [4-350]
- IPR035771: SH3 domain-containing kinase-binding protein 1, second SH3 domain (domain) [102-154]
- IPR050384: Endophilin/SH3RF (family) [91-326]
- IPR001452: SH3 domain (domain) [1-328]
- IPR035770: SH3 domain-containing kinase-binding protein 1, first SH3 domain (domain) [3-55]
- IPR035772: SH3 domain-containing kinase-binding protein 1, third SH3 domain (domain) [271-326]
Interaction partners: - E3 ubiquitin-protein ligase CBL
- B-cell linker protein
- Polyubiquitin-C
- E3 ubiquitin-protein ligase CBL-B
- Epidermal growth factor receptor
- Ubiquitin-ribosomal protein eS31 fusion protein
- Growth factor receptor-bound protein 2
- Ubiquitin-ribosomal protein eL40 fusion protein
- Polyubiquitin-B
- Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0050793 (regulation of developmental process), GO:0065008 (regulation of biological quality), GO:0048870 (cell motility), GO:0007155 (cell adhesion), GO:0032879 (regulation of localization), GO:0050794 (regulation of cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0030029 (actin filament-based process), GO:0051050 (positive regulation of transport), GO:0048522 (positive regulation of cellular process), GO:1903530 (regulation of secretion by cell), GO:1903829 (positive regulation of protein localization), GO:0051049 (regulation of transport), GO:0044085 (cellular component biogenesis), GO:0051047 (positive regulation of secretion), GO:0060341 (regulation of cellular localization), GO:0022603 (regulation of anatomical structure morphogenesis), GO:0016043 (cellular component organization), GO:0051222 (positive regulation of protein transport), GO:0030036 (actin cytoskeleton organization), GO:0031589 (cell-substrate adhesion), GO:0016477 (cell migration), GO:1903532 (positive regulation of secretion by cell), GO:0007160 (cell-matrix adhesion), GO:0006929 (substrate-dependent cell migration), GO:0030030 (cell projection organization), GO:0051223 (regulation of protein transport), GO:1904951 (positive regulation of establishment of protein localization), GO:0070201 (regulation of establishment of protein localization), GO:0051046 (regulation of secretion), GO:0032880 (regulation of protein localization), GO:0006996 (organelle organization), GO:0050708 (regulation of protein secretion), GO:0050714 (positive regulation of protein secretion), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0007010 (cytoskeleton organization), GO:0030031 (cell projection assembly), GO:0120036 (plasma membrane bounded cell projection organization), GO:0120031 (plasma membrane bounded cell projection assembly), GO:0046847 (filopodium assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0030054 (cell junction), GO:0005615 (extracellular space), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0005576 (extracellular region), GO:0043229 (intracellular organelle), GO:0070161 (anchoring junction), GO:0070062 (extracellular exosome), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0043230 (extracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0030055 (cell-substrate junction), GO:0065010 (extracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0005925 (focal adhesion), GO:1903561 (extracellular vesicle), GO:0015629 (actin cytoskeleton)