Protein: NADP(+)-dependent glutamate dehydrogenase (NADP-GDH) (EC 1.4.1.4) (A3MWK6)
Organism: Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1)
Function: Catalyzes the reversible oxidative deamination of L-glutamate to 2-oxoglutarate and ammonia, thereby playing a key role at the intersection of the carbon and nitrogen metabolic pathways. Shows a high preference for NADP(+)/NADPH as the acceptor/donor over NAD(+)/NADH. May function in vivo in the synthetic direction. Also catalyzes at very low rates the oxidative deamination of L-2-aminobutyrate, and the reductive amination of 2-oxovalerate and 2-oxobutyrate.
Sequence: MSQNGQFLEYTLQVIRRGVEMGGFPEDFYKLLSRPKRIIQVSIPVKMDNGSYEVFEGYRVQHNDALGPFKGGIRFHPEVTLADDIALAMLMTLKNSLAGLPYGGAKGAVRVDPRRLSRRELEELARGYARAVAPLIGEQLDIPAPDVGTDSQVMAWMVDEYSKLAGRNAPAVFTSKPPELWGNPVREYSTGFGVAVAAREVAKRLWGGIEGKTVAVHGAGNTGAWAAYWLEKMGAKVVAISDTRGTVVNKAGIPGEQILKVYMEKKRDKSATVLALEGEKIADSNASLYQDVDILVPAAIENVVREDNVGLVRARLVVEGANGPTTPGAERRLYERGVVVVPDILANAGGVIMSYLEWVENLQWLFWDEEETRRRLEAIMSNNVARVYARWEKEKSWTMRDAAVVTALERIYNAMKTRGWI
InterPro domains: - IPR036291: NAD(P)-binding domain superfamily (homologous_superfamily) [183-420]
- IPR006097: Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase, dimerisation domain (domain) [35-163]
- IPR033922: NAD(P) binding domain of glutamate dehydrogenase (domain) [181-413]
- IPR006096: Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase, C-terminal (domain) [182-419]
- IPR046346: Aminoacid dehydrogenase-like, N-terminal domain superfamily (homologous_superfamily) [6-182]
- IPR014362: Glutamate dehydrogenase (family) [3-421]
- IPR006095: Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase (family) [92-357]
Interaction partners: - Sulfide dehydrogenase
- isocitrate dehydrogenase
- L-glutamine synthetase
- Archaeal glutamate synthase
- Aminotransferase
- Aminotransferase
- Carbamate kinase
- Citrate synthase
- Citrate synthase
- Phosphoribosylformylglycinamidine synthase subunit PurQ
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0016491 (oxidoreductase activity), GO:0016638 (oxidoreductase activity, acting on the CH-NH2 group of donors), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0016639 (oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor), GO:0070404 (NADH binding), GO:0070403 (NAD+ binding), GO:0017076 (purine nucleotide binding), GO:0004353 (glutamate dehydrogenase [NAD(P)+] activity), GO:0030554 (adenyl nucleotide binding), GO:0051287 (NAD binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006520 (amino acid metabolic process), GO:0043603 (amide metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006082 (organic acid metabolic process), GO:0006536 (glutamate metabolic process), GO:0043436 (oxoacid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0009064 (glutamine family amino acid metabolic process), GO:0043648 (dicarboxylic acid metabolic process), GO:0006103 (2-oxoglutarate metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)