Protein: Phosphate regulon transcriptional regulatory protein PhoB (P23620)
Organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.
Subcellular location: Cytoplasm.
Sequence: MVGKTILIVDDEAPIREMIAVALEMAGYECLEAENTQQAHAVIVDRKPDLILLDWMLPGTSGIELARRLKRDELTVDIPIIMLTAKGEEDNKIQGLEVGADDYITKPFSPRELVARLKAVLRRTGPGDSEAPIEVGGLLLDPISHRVTIDGKPAEMGPTEYRLLQFFMTHQERAYTRGQLLDQVWGGNVYVEERTVDVHIRRLRKALGEVYENLVQTVRGTGYRFSTKS
InterPro domains: - IPR011879: Signal transduction response regulator, phosphate regulon transcriptional regulatory protein PhoB (family) [3-226]
- IPR001789: Signal transduction response regulator, receiver domain (domain) [4-121]
- IPR039420: Transcriptional regulatory protein WalR-like (family) [5-228]
- IPR011006: CheY-like superfamily (homologous_superfamily) [3-125]
- IPR001867: OmpR/PhoB-type DNA-binding domain (domain) [130-227]
- IPR016032: Signal transduction response regulator, C-terminal effector (homologous_superfamily) [128-226]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [129-229]
Interaction partners: - Phosphate regulon sensor protein PhoR
- Phosphate-specific transport system accessory protein PhoU homolog
- Phosphate-binding protein PstS
- Phosphate import ATP-binding protein PstB
- Alkaline phosphatase L
- histidine kinase
- Two-component sensor PhoQ
- Membrane protein component of ABC phosphate transporter
- Phosphate transport system permease protein PstA
- histidine kinase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003700 (DNA-binding transcription factor activity), GO:0003676 (nucleic acid binding), GO:0001216 (DNA-binding transcription activator activity), GO:0003677 (DNA binding), GO:0001067 (transcription regulatory region nucleic acid binding), GO:0003690 (double-stranded DNA binding), GO:0043565 (sequence-specific DNA binding), GO:0000976 (transcription cis-regulatory region binding), GO:1990837 (sequence-specific double-stranded DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0048522 (positive regulation of cellular process), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0010468 (regulation of gene expression), GO:1902680 (positive regulation of RNA biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0032993 (protein-DNA complex)