Protein: RNA-binding protein OPG065 (P21605)
Organism: Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
Function: RNA-binding protein that plays a role in the inhibition of multiple cellular antiviral responses activated by double-stranded RNA (dsRNA), such as inhibition of PKR activation, necroptosis, and IFN- mediated antiviral activities. Recognizes and binds Z-RNA structures via its Z-binding domain and dsRNA via its DRBM domain: RNA-binding activity is required to escape host ZBP1-dependent necroptosis. Mechanistically, the Z- binding domain binds Z-RNAs that are produced during vaccinia virus infection, thereby competing with Z-RNA detection by host ZBP1, suppressing ZBP1-dependent necroptosis. Acts as a key inhibitor of the interferon response by blocking the phosphorylation and subsequent activation of IRF3 and IRF7 kinases that are required for interferon-alpha gene expression. Inhibits NF- kappa-B activation and the ubiquitin-like protein ISG15, which is an early antiviral protein. The binding with host ISG15 subsequently blocks host ISGylation
Sequence: MSKIYIDERSNAEIVCEAIKTIGIEGATAAQLTRQLNMEKREVNKALYDLQRSAMVYSSDDIPPRWFMTTEADKPDADAMADVIIDDVSREKSMREDHKSFDDVIPAKKIIDWKGANPVTVINEYCQITRRDWSFRIESVGPSNSPTFYACVDIDGRVFDKADGKSKRDAKNNAAKLAVDKLLGYVIIRF
InterPro domains: - IPR009179: Protein E3 (family) [3-190]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [1-78]
- IPR014720: Double-stranded RNA-binding domain (domain) [117-184]
- IPR036390: Winged helix DNA-binding domain superfamily (homologous_superfamily) [10-69]
- IPR042371: Z-binding domain (domain) [3-72]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0003725 (double-stranded RNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0023057 (negative regulation of signaling), GO:0009058 (biosynthetic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009892 (negative regulation of metabolic process), GO:0044237 (cellular metabolic process), GO:0009607 (response to biotic stimulus), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0023051 (regulation of signaling), GO:0065009 (regulation of molecular function), GO:0048523 (negative regulation of cellular process), GO:0051707 (response to other organism), GO:0048585 (negative regulation of response to stimulus), GO:0044238 (primary metabolic process), GO:0050790 (regulation of catalytic activity), GO:0009968 (negative regulation of signal transduction), GO:0010648 (negative regulation of cell communication), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0043207 (response to external biotic stimulus), GO:0019538 (protein metabolic process), GO:0009966 (regulation of signal transduction), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0098542 (defense response to other organism), GO:0010646 (regulation of cell communication), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0009615 (response to virus), GO:0009059 (macromolecule biosynthetic process), GO:0043086 (negative regulation of catalytic activity), GO:1901362 (organic cyclic compound biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0051248 (negative regulation of protein metabolic process), GO:0043604 (amide biosynthetic process), GO:1902532 (negative regulation of intracellular signal transduction), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0043412 (macromolecule modification), GO:0010563 (negative regulation of phosphorus metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0051338 (regulation of transferase activity), GO:0010467 (gene expression), GO:1902531 (regulation of intracellular signal transduction), GO:0051174 (regulation of phosphorus metabolic process), GO:0043408 (regulation of MAPK cascade), GO:0031399 (regulation of protein modification process), GO:0031400 (negative regulation of protein modification process), GO:0019220 (regulation of phosphate metabolic process), GO:0043549 (regulation of kinase activity), GO:0051348 (negative regulation of transferase activity), GO:0045936 (negative regulation of phosphate metabolic process), GO:0043409 (negative regulation of MAPK cascade), GO:0042325 (regulation of phosphorylation), GO:0001933 (negative regulation of protein phosphorylation), GO:0001932 (regulation of protein phosphorylation), GO:0033673 (negative regulation of kinase activity), GO:0042326 (negative regulation of phosphorylation), GO:0045859 (regulation of protein kinase activity), GO:0071900 (regulation of protein serine/threonine kinase activity), GO:0006469 (negative regulation of protein kinase activity), GO:0071901 (negative regulation of protein serine/threonine kinase activity), GO:0043405 (regulation of MAP kinase activity)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus)