Protein: Cytoplasmic tRNA 2-thiolation protein 1 (Q94480)
Organism: Dictyostelium discoideum (Social amoeba)
Function: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated urm1 onto the uridine of tRNAs at wobble position
Subcellular location: Cytoplasm
Sequence: MENTATKKKSKLCEVCNVSRPVLKRPKTGEMICKECFYTVFEDEIHHTIISNNLFKRGDRVAIGASGGKDSTVLAEIMTLLNKKYDYGLDLFLLSIDEGITGYRDDSLETVKRNQEQYQIPLKILSYKELYNWTMDDIVKEIGLKGNCTFCGVFRRQALDRGAVMLKANKIVTGHNADDIAETVLMNLLRGDIPRLQRCVNIITGSEGALPRSKPFKYTYQKEIVMYAYFKKLDYFSTECIYAPNAYRGHARDFLKDLEAVRPSIIIDIIHSAENFHFREENKMPVQRNCIQCGYICSNDICMACDLLKNLNSGLAKVKLSINLKRGKDNNNNSNNNDNNNTLKLESTSAATTTTTTVSN
InterPro domains: - IPR056369: Cytoplasmic tRNA 2-thiolation protein 1-like, ATP-binding domain (domain) [41-246]
- IPR011063: tRNA(Ile)-lysidine/2-thiocytidine synthase, N-terminal (domain) [61-239]
- IPR014729: Rossmann-like alpha/beta/alpha sandwich fold (homologous_superfamily) [12-334]
- IPR020554: Uncharacterised protein family UPF0021, conserved site (conserved_site) [33-70]
- IPR000541: Cytoplasmic tRNA 2-thiolation protein 1 (family) [11-323]
- IPR032442: Cytoplasmic tRNA 2-thiolation protein 1, C-terminal (domain) [288-318]
- IPR035107: tRNA thiolation protein, TtcA/Ctu1 type (family) [12-318]
Interaction partners: - Cytoplasmic tRNA 2-thiolation protein 2
- Elongator complex protein 3
- Adenylyltransferase and sulfurtransferase MOCS3
- DNA replication licensing factor mcm5
- Molybdopterin synthase catalytic subunit
- Molybdenum cofactor biosynthesis protein 1
- Molybdopterin synthase sulfur carrier subunit
- Iron-sulfur cluster assembly 2 homolog, mitochondrial
- Iron-sulfur cluster assembly 1 homolog, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0016740 (transferase activity), GO:0140098 (catalytic activity, acting on RNA), GO:0016782 (transferase activity, transferring sulphur-containing groups), GO:0016783 (sulfurtransferase activity), GO:0140101 (catalytic activity, acting on a tRNA)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0009451 (RNA modification), GO:0006400 (tRNA modification), GO:0034470 (ncRNA processing), GO:0034660 (ncRNA metabolic process), GO:0002097 (tRNA wobble base modification), GO:0006399 (tRNA metabolic process), GO:0008033 (tRNA processing), GO:0002098 (tRNA wobble uridine modification)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0031982 (vesicle), GO:0030139 (endocytic vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0097708 (intracellular vesicle), GO:0045335 (phagocytic vesicle)