Protein: Cytosolic Fe-S cluster assembly factor NUBP1 (Nucleotide-binding protein 1) (NBP 1) (P53384)
Organism: Homo sapiens (Human)
Function: Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Implicated in the regulation of centrosome duplication (By similarity). Negatively regulates cilium formation and structure (By similarity).
Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03038, ECO:0000269|PubMed:18573874, ECO:0000269|PubMed:29848660}. Nucleus {ECO:0000250|UniProtKB:Q9R060}. Cell projection {ECO:0000250|UniProtKB:Q9R060}. Cytoplasm, cytoskeleton, cilium axoneme {ECO:0000250|UniProtKB:Q9R060}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q9R060}. Cytoplasm, cytoskeleton, microtubule organizing center {ECO:0000250|UniProtKB:Q9R060}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:Q9R060}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Enriched in centrioles of microtubule asters during prophase, prometaphase and telophase stages of mitosis. Localized at centrioles and in the nucleus at interphase. Colocalizes with nubp-2 at prometaphase. Specifically localizes to the axenome of motile cilia as opposed to primary non-motile cilia. Localization is independent of NUBP2 and KIFC1. {ECO:0000250|UniProtKB:Q9R060}.
Sequence: MEEVPHDCPGADSAQAGRGASCQGCPNQRLCASGAGATPDTAIEEIKEKMKTVKHKILVLSGKGGVGKSTFSAHLAHGLAEDENTQIALLDIDICGPSIPKIMGLEGEQVHQSGSGWSPVYVEDNLGVMSVGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGEVDYLIVDTPPGTSDEHLSVVRYLATAHIDGAVIITTPQEVSLQDVRKEINFCRKVKLPIIGVVENMSGFICPKCKKESQIFPPTTGGAELMCQDLEVPLLGRVPLDPLIGKNCDKGQSFFIDAPDSPATLAYRSIIQRIQEFCNLHQSKEENLISS
InterPro domains: - IPR028601: Cytosolic Fe-S cluster assembly factor NUBP1/Nbp35 (family) [1-308]
- IPR003593: AAA+ ATPase domain (domain) [54-210]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [43-317]
- IPR033756: Flagellum site-determining protein YlxH/ Fe-S cluster assembling factor NBP35 (family) [53-303]
- IPR019591: Mrp/NBP35 ATP-binding protein (family) [8-311]
- IPR000808: Iron-sulfur cluster carrier protein-like, conserved site (conserved_site) [162-178]
Interaction partners: - Probable cytosolic iron-sulfur protein assembly protein CIAO1
- Anamorsin
- Cytosolic iron-sulfur assembly component 3
- Iron-sulfur clusters transporter ABCB7, mitochondrial
- MMS19 nucleotide excision repair protein homolog
- Cytosolic Fe-S cluster assembly factor NUBP2
- Cytosolic iron-sulfur assembly component 2B
- NADPH-dependent diflavin oxidoreductase 1
- FAD-dependent oxidoreductase domain-containing protein 1
- Iron-sulfur cluster assembly enzyme ISCU
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0016887 (ATP hydrolysis activity), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0042802 (identical protein binding), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0016462 (pyrophosphatase activity), GO:0017111 (ribonucleoside triphosphate phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0051641 (cellular localization), GO:0022402 (cell cycle process), GO:0050794 (regulation of cellular process), GO:0007049 (cell cycle), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0007017 (microtubule-based process), GO:0048523 (negative regulation of cellular process), GO:0051640 (organelle localization), GO:0051726 (regulation of cell cycle), GO:0007059 (chromosome segregation), GO:0044085 (cellular component biogenesis), GO:1903047 (mitotic cell cycle process), GO:0046907 (intracellular transport), GO:0051656 (establishment of organelle localization), GO:0007051 (spindle organization), GO:0006810 (transport), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0000226 (microtubule cytoskeleton organization), GO:0032886 (regulation of microtubule-based process), GO:0051128 (regulation of cellular component organization), GO:0010564 (regulation of cell cycle process), GO:0000278 (mitotic cell cycle), GO:0044087 (regulation of cellular component biogenesis), GO:0051647 (nucleus localization), GO:1902850 (microtubule cytoskeleton organization involved in mitosis), GO:0051225 (spindle assembly), GO:0070507 (regulation of microtubule cytoskeleton organization), GO:0043254 (regulation of protein-containing complex assembly), GO:0030705 (cytoskeleton-dependent intracellular transport), GO:0140014 (mitotic nuclear division), GO:0007097 (nuclear migration), GO:0033043 (regulation of organelle organization), GO:0046605 (regulation of centrosome cycle), GO:0007052 (mitotic spindle organization), GO:0006996 (organelle organization), GO:0000070 (mitotic sister chromatid segregation), GO:0099111 (microtubule-based transport), GO:0072384 (organelle transport along microtubule), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0007010 (cytoskeleton organization), GO:0051493 (regulation of cytoskeleton organization), GO:0000819 (sister chromatid segregation), GO:0060236 (regulation of mitotic spindle organization), GO:0048285 (organelle fission), GO:0090307 (mitotic spindle assembly), GO:0051276 (chromosome organization), GO:0000280 (nuclear division), GO:0140694 (non-membrane-bounded organelle assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0005737 (cytoplasm)