Protein: Diacylglycerol kinase alpha (DAG kinase alpha) (EC 2.7.1.107) (EC 2.7.1.93) (80 kDa diacylglycerol kinase) (Diglyceride kinase alpha) (DGK-alpha) (P20192)
Organism: Sus scrofa (Pig)
Function: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group. Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol (By similarity).
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:8809050}.
Sequence: MSKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDSVPSHLSLALFQSFQTSYCSEETVKRDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQTHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKVSKTSQKTTDDLNLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFREVPDYRILVCGGDGTVGWILETIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLEASKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGAMAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC
InterPro domains: - IPR046349: C1-like domain superfamily (homologous_superfamily) [185-323]
- IPR038199: DGK type I, N-terminal domain superfamily (homologous_superfamily) [1-114]
- IPR001206: Diacylglycerol kinase, catalytic domain (domain) [371-505]
- IPR002219: Protein kinase C-like, phorbol ester/diacylglycerol-binding domain (domain) [204-320]
- IPR011992: EF-hand domain pair (homologous_superfamily) [1-182]
- IPR000756: Diacylglycerol kinase, accessory domain (domain) [519-700]
- IPR002048: EF-hand domain (domain) [109-189]
- IPR017438: Inorganic polyphosphate/ATP-NAD kinase, N-terminal (homologous_superfamily) [372-502]
- IPR029477: Diacylglycerol kinase type I, N-terminal (domain) [6-87]
- IPR047469: Diacylglycerol kinase alpha, second protein kinase C conserved region 1 (domain) [269-330]
- IPR018247: EF-Hand 1, calcium-binding site (binding_site) [122-179]
- IPR016064: NAD kinase/diacylglycerol kinase-like domain superfamily (homologous_superfamily) [375-713]
- IPR037607: Diacylglycerol kinase (family) [161-720]
Interaction partners: - Acyltransferase
- Lipoprotein lipase
- Dihydroxyacetone phosphate acyltransferase
- Glycerol-3-phosphate dehydrogenase
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma
- Phosphoinositide phospholipase C
- Phosphoinositide phospholipase C.
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
- Phospholipase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0008289 (lipid binding), GO:0005543 (phospholipid binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0016301 (kinase activity), GO:0004143 (diacylglycerol kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006793 (phosphorus metabolic process), GO:0046486 (glycerolipid metabolic process), GO:0006644 (phospholipid metabolic process), GO:0090407 (organophosphate biosynthetic process), GO:0030258 (lipid modification), GO:0006638 (neutral lipid metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0008610 (lipid biosynthetic process), GO:0045017 (glycerolipid biosynthetic process), GO:0006650 (glycerophospholipid metabolic process), GO:0046834 (lipid phosphorylation), GO:0008654 (phospholipid biosynthetic process), GO:0046474 (glycerophospholipid biosynthetic process), GO:0006639 (acylglycerol metabolic process), GO:0016310 (phosphorylation), GO:0046339 (diacylglycerol metabolic process), GO:0006654 (phosphatidic acid biosynthetic process), GO:0046473 (phosphatidic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)