Protein: Cofilin (Actin-depolymerizing factor 1) (Q03048)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. Binding to F-actin is regulated by tropomyosin. It is the major component of intranuclear and cytoplasmic actin rods. Required for accumulation of actin at the cell division site via depolymerizing actin at the cell ends. In association with myosin II has a role in the assembly of the contractile ring via severing actin filaments. Involved in the maintenance of the contractile ring once formed. In association with profilin and capping protein, has a role in the mitotic reorganization of the actin cytoskeleton. In effect, yeast cofilin increases the rate of actin polymerization by making new ends available for actin subunit addition. Such a protein complex is important for the polarized growth of yeast cells.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:10231390}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:10231390}. Nucleus matrix {ECO:0000269|PubMed:10231390}. Note=Throughout the cytoplasm (but not on the cytoplasmic cables) and major component of the cortical actin cytoskeleton.
Sequence: MSRSGVAVADESLTAFNDLKLGKKYKFILFGLNDAKTEIVVKETSTDPSYDAFLEKLPENDCLYAIYDFEYEINGNEGKRSKIVFFTWSPDTAPVRSKMVYASSKDALRRALNGVSTDVQGTDFSEVSYDSVLERVSRGAGSH
InterPro domains: - IPR029006: ADF-H/Gelsolin-like domain superfamily (homologous_superfamily) [1-143]
- IPR017904: ADF/Cofilin (family) [2-136]
- IPR002108: Actin-depolymerising factor homology domain (domain) [5-137]
Interaction partners: - Actin
- Profilin
- Actin-interacting protein 1
- Actin-related protein 2
- Actin-related protein 3
- Adenylyl cyclase-associated protein
- Twinfilin-1
- Coronin-like protein
- Fimbrin
- F-actin-capping protein subunit beta
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0044877 (protein-containing complex binding), GO:0005515 (protein binding), GO:0051015 (actin filament binding), GO:0008092 (cytoskeletal protein binding), GO:0003779 (actin binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0030029 (actin filament-based process), GO:0016043 (cellular component organization), GO:0030036 (actin cytoskeleton organization), GO:0043933 (protein-containing complex organization), GO:0006996 (organelle organization), GO:0022411 (cellular component disassembly), GO:0097435 (supramolecular fiber organization), GO:0007015 (actin filament organization), GO:0007010 (cytoskeleton organization), GO:0008154 (actin polymerization or depolymerization), GO:0032984 (protein-containing complex disassembly), GO:0051261 (protein depolymerization), GO:0030042 (actin filament depolymerization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0061645 (endocytic patch), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0043229 (intracellular organelle), GO:0005938 (cell cortex), GO:0030479 (actin cortical patch), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0030863 (cortical cytoskeleton), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0030864 (cortical actin cytoskeleton), GO:0005634 (nucleus), GO:0015629 (actin cytoskeleton)