Protein: Deubiquitinating protein VCPIP1 (A0A8M2BJ69)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Deubiquitinating enzyme involved in DNA repair and reassembly of the Golgi apparatus and the endoplasmic reticulum following mitosis. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. Plays a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP. Also involved in DNA repair following phosphorylation by ATM or ATR: acts by catalyzing deubiquitination of SPRTN, thereby promoting SPRTN recruitment to chromatin and subsequent proteolytic cleavage of covalent DNA-protein cross-links (DPCs). Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains
Subcellular location: Cytoplasm. Endoplasmic reticulum. Golgi apparatus, Golgi stack. Nucleus
Sequence: MSLIPGSKQKDRRILCGMCPDPQCQAKLIFPAHTSMSIECTECGQRHEQKNLANVEEVTNPDVVLHNLLRNALLGVPGPPKKGSELVKVMGLSNYHCKLLSPILTRYGMDKQTGTAKLLKEMNQGDIFDCSLLGDRAFLIEQEHVSTVGYGRDRSGSLIYLHDTLEEIKKANGNRECLIPVHVDGDGHCLVHAVSRALVGRELFWHALRENLKLHFKQNLDRYKALFQDFIDAAEWEDIINECDPLFVPPEGVPLGLRNIHIFGLANVLHRPVILLDSLSGMRSSGDYSATFLPGLIPEEQCRGKDGTLNKPICIAWSSSGRNHYLPLVGIKGLALPRLPAALLPKAWGVPQELIRKYVSLDPSGSCVIGGDRSLQDKYLLRLVSAMEDVFMDKHSIHPALVADVHQYIYRRTGVIGVQPEEVTEAAKKSIQEGRLHRCLVCNALSELHVPAEWLIPGGKLYNLAKSTHGTLRADKNYSFPLNSLVCSYNPEKDVLVPDYKLSSLTTCTWCHGTSVRRVRGDGSVVYLDGDRTNTPSQGGKCGCGFKHFWEGKEYDNLPEAFPITLEWAGRVVRETVYWFQYEIDPTLNSNVYDVTMRLVTKHFPGEFGSEILVQKVVNTILHHTAKKSQDEYNPVAIEGAHVQNKVDGGESSAHPPTKIILTGQKGKTLHKEELVMSKTERVLQHSISEQAALSQRRSTDRVRHQDPRPSSPSSSSSSAPPTPTKVPPTSGEKKIRVTTSDGRQAMLTLQPHTTYSDLQNSIIQVFNLSPGQLCIRHGFPPRELPPPPPEDQNRPVALQHGDRISVEVLKESSADPQVTQTHTHSPQTHAHSDPRLSHSSSRELQDNIDLEMSSLCLLAALMGEDVWSYAKKLPHLFQQGGVFYNIVKKDMGLLDGKHCTLPHLSGKTFVFNATEERLELCVDTAGHFPVGPDVEELVQEALSQLRSDAASRSREGSPAHSLRLGSGGAVRKKEQNITAFQGKGHSLGSAPPEHPPIRRQHSSGVDLSGSARGEGLTLSGEDLVRVAPGMLTLREGRGMGLEPAVIEAQRQRLQEMVSNIQASMDKHLRQHTAARNDGKEEETPDKPEEMESHGPEPPNTFEPMDQS
InterPro domains: - IPR003323: OTU domain (domain) [178-331]
- IPR048857: OTU1, Ubl domain (domain) [735-785]
- IPR039087: Deubiquitinating protein VCPIP1 (family) [5-1078]
- IPR045827: Deubiquitinating protein VCPIP1, N-terminal (domain) [8-172]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0008233 (peptidase activity), GO:0008234 (cysteine-type peptidase activity), GO:0019783 (ubiquitin-like protein peptidase activity), GO:0101005 (deubiquitinase activity), GO:0004843 (cysteine-type deubiquitinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044085 (cellular component biogenesis), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0016043 (cellular component organization), GO:0043170 (macromolecule metabolic process), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0010256 (endomembrane system organization), GO:0006996 (organelle organization), GO:0061024 (membrane organization), GO:0022607 (cellular component assembly), GO:0007029 (endoplasmic reticulum organization), GO:0007030 (Golgi organization), GO:0048308 (organelle inheritance), GO:0090158 (endoplasmic reticulum membrane organization), GO:0070647 (protein modification by small protein conjugation or removal), GO:0048284 (organelle fusion), GO:0061025 (membrane fusion), GO:0032446 (protein modification by small protein conjugation), GO:0090174 (organelle membrane fusion), GO:0016567 (protein ubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)