Protein: Cytochrome P450 710A1 (EC 1.14.19.41) (C-22 sterol desaturase) (O64697)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Required to form the C-22 double bond in the sterol side chain. Possesses in vitro C-22 desaturase activity toward beta-sitosterol and produces stigmasterol. No activity with campesterol.
Subcellular location: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
Sequence: MVFSVSIFASLAPYLISAFLLFLLVEQLSYLFKKRNIPGPFFVPPIIGNAVALVRDPTSFWDKQSSTANISGLSANYLIGKFIVYIRDTELSHQIFSNVRPDAFHLIGHPFGKKLFGDHNLIYMFGEDHKSVRRQLAPNFTPKALSTYSALQQLVILRHLRQWEGSTSGGSRPVSLRQLVRELNLETSQTVFVGPYLDKEAKNRFRTDYNLFNLGSMALPIDLPGFAFGEARRAVKRLGETLGICAGKSKARMAAGEEPACLIDFWMQAIVAENPQPPHSGDEEIGGLLFDFLFAAQDASTSSLLWAVTLLDSEPEVLNRVREEVAKIWSPESNALITVDQLAEMKYTRSVAREVIRYRPPATMVPHVAAIDFPLTETYTIPKGTIVFPSVFDSSFQGFTEPDRFDPDRFSETRQEDQVFKRNFLAFGWGPHQCVGQRYALNHLVLFIAMFSSLLDFKRLRSDGCDEIVYCPTISPKDGCTVFLSRRVAKYPNFS
InterPro domains: - IPR001128: Cytochrome P450 (family) [77-451]
- IPR002401: Cytochrome P450, E-class, group I (family) [304-457]
- IPR036396: Cytochrome P450 superfamily (homologous_superfamily) [30-487]
- IPR017972: Cytochrome P450, conserved site (conserved_site) [427-436]
Interaction partners: - Delta
- 24-methylenesterol C-methyltransferase 2
- Delta
- Methylsterol monooxygenase 2-1
- Cycloeucalenol cycloisomerase
- Delta
- Methylsterol monooxygenase 2-2
- Cycloartenol-C-24-methyltransferase
- 7-dehydrocholesterol reductase
- Serine carboxypeptidase-like 8
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0016717 (oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009058 (biosynthetic process), GO:0009653 (anatomical structure morphogenesis), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0044238 (primary metabolic process), GO:0032989 (cellular component morphogenesis), GO:1901576 (organic substance biosynthetic process), GO:0044085 (cellular component biogenesis), GO:0006629 (lipid metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0048229 (gametophyte development), GO:0010927 (cellular component assembly involved in morphogenesis), GO:0006631 (fatty acid metabolic process), GO:0009555 (pollen development), GO:0010208 (pollen wall assembly), GO:0043436 (oxoacid metabolic process), GO:0016053 (organic acid biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0045229 (external encapsulating structure organization), GO:0008610 (lipid biosynthetic process), GO:0022607 (cellular component assembly), GO:0019752 (carboxylic acid metabolic process), GO:0006633 (fatty acid biosynthetic process), GO:0010584 (pollen exine formation), GO:0051791 (medium-chain fatty acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0072330 (monocarboxylic acid biosynthetic process), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)