Protein: Chondroitin sulfate N-acetylgalactosaminyltransferase 1 (CsGalNAcT-1) (EC 2.4.1.174) (Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 1) (Beta4GalNAcT-1) (Q8TDX6)
Organism: Homo sapiens (Human)
Function: Transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA). Required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains. Important role in chondroitin chain biosynthesis in cartilage formation and subsequent endochondral ossification. Moreover, is involved in the metabolism of aggrecan (By similarity).
Subcellular location: Golgi apparatus, Golgi stack membrane {ECO:0000305|PubMed:11788602}; Single-pass type II membrane protein {ECO:0000305|PubMed:11788602}.
Sequence: MMMVRRGLLAWISRVVVLLVLLCCAISVLYMLACTPKGDEEQLALPRANSPTGKEGYQAVLQEWEEQHRNYVSSLKRQIAQLKEELQERSEQLRNGQYQASDAAGLGLDRSPPEKTQADLLAFLHSQVDKAEVNAGVKLATEYAAVPFDSFTLQKVYQLETGLTRHPEEKPVRKDKRDELVEAIESALETLNSPAENSPNHRPYTASDFIEGIYRTERDKGTLYELTFKGDHKHEFKRLILFRPFGPIMKVKNEKLNMANTLINVIVPLAKRVDKFRQFMQNFREMCIEQDGRVHLTVVYFGKEEINEVKGILENTSKAANFRNFTFIQLNGEFSRGKGLDVGARFWKGSNVLLFFCDVDIYFTSEFLNTCRLNTQPGKKVFYPVLFSQYNPGIIYGHHDAVPPLEQQLVIKKETGFWRDFGFGMTCQYRSDFINIGGFDLDIKGWGGEDVHLYRKYLHSNLIVVRTPVRGLFHLWHEKRCMDELTPEQYKMCMQSKAMNEASHGQLGMLVFRHEIEAHLRKQKQKTSSKKT
InterPro domains: - IPR029044: Nucleotide-diphospho-sugar transferases (homologous_superfamily) [250-531]
- IPR051227: Chondroitin sulfate glycosyltransferase (family) [170-508]
- IPR008428: Chondroitin N-acetylgalactosaminyltransferase (family) [73-507]
Interaction partners: - Chondroitin sulfate synthase 2
- Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
- Chondroitin sulfate N-acetylgalactosaminyltransferase 2
- Chondroitin sulfate glucuronyltransferase
- Chondroitin sulfate synthase 1
- Chondroitin sulfate synthase 3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016757 (glycosyltransferase activity), GO:0016758 (hexosyltransferase activity), GO:0008194 (UDP-glycosyltransferase activity), GO:0008376 (acetylgalactosaminyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0005975 (carbohydrate metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0030204 (chondroitin sulfate metabolic process), GO:0009100 (glycoprotein metabolic process), GO:0044272 (sulfur compound biosynthetic process), GO:0016051 (carbohydrate biosynthetic process), GO:0005976 (polysaccharide metabolic process), GO:0034637 (cellular carbohydrate biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0044264 (cellular polysaccharide metabolic process), GO:0006022 (aminoglycan metabolic process), GO:0006029 (proteoglycan metabolic process), GO:0033692 (cellular polysaccharide biosynthetic process), GO:0000271 (polysaccharide biosynthetic process), GO:0030203 (glycosaminoglycan metabolic process), GO:0009101 (glycoprotein biosynthetic process), GO:0030206 (chondroitin sulfate biosynthetic process), GO:0006023 (aminoglycan biosynthetic process), GO:0050654 (chondroitin sulfate proteoglycan metabolic process), GO:0006024 (glycosaminoglycan biosynthetic process), GO:1903510 (mucopolysaccharide metabolic process), GO:0030166 (proteoglycan biosynthetic process), GO:0050650 (chondroitin sulfate proteoglycan biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0031090 (organelle membrane), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0000139 (Golgi membrane)