Protein: Endoribonuclease Dicer homolog 3 (EC 3.1.26.-) (Dicer-like protein 3) (AtDCL3) (Q9LXW7)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Involved in the processing of repeat-associated small interfering RNAs (ra-siRNAs, derived from heterochromatin and DNA repeats such as transposons) by cleaving small dsRNAs into 24 nucleotide ra-siRNAs. Plays a role in antiviral RNA silencing. Involved in the production of viral siRNAs derived from the cabbage leaf curl virus (CaLCuV) and tobacco rattle virus (TRV). Targeted by the viral silencing suppressor (VSR) protein 2b of the cucumber mosaic virus (CMV) that inactivates DCL3 function in RNA silencing. Acts redundantly with DICER-LIKE 1 (DCL1) to promote flowering via repression of FLOWERING LOCUS C (FLC). Does not seem to be involved in microRNAs (miRNAs) processing.
Subcellular location: Nucleus, nucleolus {ECO:0000269|PubMed:15024409, ECO:0000269|PubMed:16839878}.
Sequence: MHSSLEPEKMEEGGGSNSLKRKFSEIDGDQNLDSVSSPMMTDSNGSYELKVYEVAKNRNIIAVLGTGIDKSEITKRLIKAMGSSDTDKRLIIFLAPTVNLVKQQCCEIRALVNLKVEEYFGAKGVDKWTSQRWDEEFSKHDVLVMTPQILLDVLRSAFLKLEMVCLLIIDECHHTTGNHPYAKLMKEFYHESTSKPKIFGLTASAVIRKGIVSSPSNYAAQVSELERLMDSKIFNPEEREGVEKFATTVKEGPILYNPSPSCSLELKEKLETSHLKFDASLRRLQELGKDSFLNMDNKFETYQKRLSIDYREILHCLDNLGLICAHLAAEVCLEKISDTKEESETYKECSMVCKEFLEDILSTIGVYLPQDDKSLVDLQQNHLSAVISGHVSPKLKELFHLLDSFRGDKQKQCLILVERIITAKVIERFVKKEASLAYLNVLYLTENNPSTNVSAQKMQIEIPDLFQHGKVNLLFITDVVEEGFQVPDCSCMVCFDLPKTMCSYSQSQKHAKQSNSKSIMFLERGNPKQRDHLHDLMRREVLIQDPEAPNLKSCPPPVKNGHGVKEIGSMVIPDSNITVSEEAASTQTMSDPPSRNEQLPPCKKLRLDNNLLQSNGKEKVASSKSKSSSSAAGSKKRKELHGTTCANALSGTWGENIDGATFQAYKFDFCCNISGEVYSSFSLLLESTLAEDVGKVEMDLYLVRKLVKASVSPCGQIRLSQEELVKAKYFQQFFFNGMFGKLFVGSKSQGTKREFLLQTDTSSLWHPAFMFLLLPVETNDLASSATIDWSAINSCASIVEFLKKNSLLDLRDSDGNQCNTSSGQEVLLDDKMEETNLIHFANASSDKNSLEELVVIAIHTGRIYSIVEAVSDSSAMSPFEVDASSGYATYAEYFNKKYGIVLAHPNQPLMKLKQSHHAHNLLVDFNEEMVVKTEPKAGNVRKRKPNIHAHLPPELLARIDVPRAVLKSIYLLPSVMHRLESLMLASQLREEIDCSIDNFSISSTSILEAVTTLTCPESFSMERLELLGDSVLKYVASCHLFLKYPDKDEGQLSRQRQSIISNSNLHRLTTSRKLQGYIRNGAFEPRRWTAPGQFSLFPVPCKCGIDTREVPLDPKFFTENMTIKIGKSCDMGHRWVVSKSVSDCAEALIGAYYVSGGLSASLHMMKWLGIDVDFDPNLVVEAINRVSLRCYIPKEDELIELERKIQHEFSAKFLLKEAITHSSLRESYSYERLEFLGDSVLDFLITRHLFNTYEQTGPGEMTDLRSACVNNENFAQVAVKNNLHTHLQRCATVLETQINDYLMSFQKPDETGRSIPSIQGPKALGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTPDKLQLPPYRELNELCDSLGYFFRVKCSNDGVKAQATIQLQLDDVLLTGDGSEQTNKLALGKAASHLLTQLEKRNISRKTSLGDNQSSMDVNLACNHSDRETLTSETTEIQSIVIPFIGPINMKKGGPRGTLHEFCKKHLWPMPTFDTSEEKSRTPFEFIDGGEKRTSFSSFTSTITLRIPNREAVMYAGEARPDKKSSFDSAVVELLYELERRKIVIIQK
InterPro domains: - IPR001650: Helicase, C-terminal domain-like (domain) [394-562]
- IPR003100: PAZ domain (domain) [834-984]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [41-543]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [45-231]
- IPR000999: Ribonuclease III domain (domain) [985-1363]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [53-205]
- IPR036389: Ribonuclease III, endonuclease domain superfamily (homologous_superfamily) [976-1360]
- IPR036085: PAZ domain superfamily (homologous_superfamily) [802-918]
Interaction partners: - Protein argonaute 4
- RNA-dependent RNA polymerase 2
- Protein argonaute 6
- Double-stranded RNA-binding protein 3
- RNA-dependent RNA polymerase 6
- Protein argonaute 1
- Protein argonaute 2
- Protein argonaute 9
- Protein DCL homolog, chloroplastic
- Protein argonaute 7
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0003725 (double-stranded RNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0032259 (methylation), GO:0009607 (response to biotic stimulus), GO:0006807 (nitrogen compound metabolic process), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0051707 (response to other organism), GO:0044238 (primary metabolic process), GO:0009892 (negative regulation of metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0043207 (response to external biotic stimulus), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0006952 (defense response), GO:0060255 (regulation of macromolecule metabolic process), GO:0043414 (macromolecule methylation), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0098542 (defense response to other organism), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0051607 (defense response to virus), GO:0006259 (DNA metabolic process), GO:0010467 (gene expression), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression), GO:0006306 (DNA methylation), GO:0016070 (RNA metabolic process), GO:0006304 (DNA modification), GO:0031047 (RNA-mediated gene silencing), GO:0006396 (RNA processing), GO:0006305 (DNA alkylation), GO:0034470 (ncRNA processing), GO:0070918 (regulatory ncRNA processing), GO:0034660 (ncRNA metabolic process), GO:0030422 (siRNA processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)