Protein: Elongation factor 3 (P25997)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Ribosome-dependent ATPase that functions in cytoplasmic translation elongation (By similarity). Required for the ATP-dependent release of deacylated tRNA from the ribosomal E-site during protein biosynthesis (By similarity). Stimulates the eEF1A-dependent binding of aminoacyl-tRNA to the ribosomal A-site, which has reduced affinity for tRNA as long as the E-site is occupied (By similarity). Assists translation termination by stimulating the release of nascent protein from the ribosome by release factors (By similarity)
Subcellular location: Cytoplasm
Sequence: MSAASESKYSTEVLSELLSKLQVADNKDEAASNISTFLNSSIVEHDVPVEFFEDLKKQIQSKDAKVSLAALDAYKHIASTNGLSPSVEPYVVDLVSEVAVKAGDKNKDVQTAASDALLAIASAITPTAVKAILPKLIDNLTNTNKWTEKVAILRAVSQLVDTAKAQIALRMPELIPVLSESMWDTKKEVKEAATATMTKSTETIDNKDIEKFIPQLISCIAKPTEVPETVHLLGATTFVSEVTMATLSIMAPLLSRGLAERDTAIKRKAAVIVDNMCKLVEDPQIVAPFMDKLLPGLKNNFANMADPEAREVTQRALNTLRRVGAVGENDSIPEVSTAGDIDVTLNEFNKLVADKKIAKRFDVALNYIAAIAGDLVDEREIQPEAWLQNVLPFATIFLHEKEAKEIIEEFRKRAIDNIPQPPSFEDEEDEGEDLCNCEFSLAYGAKILLNKTQFRLKRNRRYGLCGPNGAGKSTLMRAIANGQVEGFPTQDECKTVYVEHDIDGTHADTTVVEFVIEDGEVGLTKDVVVDKLREFNFSDEMINMPIQSLSGGWKMKLALARAVLKNADILLLDEPTNHLDTVNVAWLVNYLNTCGITSIIVSHDSGFLDNVTQYIIHYEGFKLRKYKGNLSEFVKKCPSAQSYYELGASDLEFRFPEPGFLEGVKTKQKAIVKVSNMSFQYPGTSKPQIQDINFQCSLSSRIAVIGPNGAGKSTLINVLTGELLPTTGEVYVHENCRIAYIKQHAFAHIDNHLDKTPSEYIQWRFQTGEDRETMDRASRQINEEDEQNMNKIFKIEGTPRRIAGIHARRKFKNSYEYEISWMLGENIGMKNERWVPMMSVDNTWLPRGELMETHAKLVAEVDMKEALASGQFRPLTRKEIEEHCAMLGLDAELVSHSRIRGLSGGQKVKLVLAACTWQRPHLIVLDEPTNYLDRDSLGALSKALKAFEGGIVIITHSAEFTKDLTEEVWAVLDGRMTPSGHNWVQGQGSGPRIEKKDDEEEDKFDAMGNKIAAAKKKKKLSSAELRKKKKERMKKKKELGDAYVSSDEEF
InterPro domains: - IPR047036: Elongation factor 3, four helical bundle domain superfamily (homologous_superfamily) [332-421]
- IPR016024: Armadillo-type fold (homologous_superfamily) [29-1041]
- IPR011989: Armadillo-like helical (homologous_superfamily) [2-331]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [423-980]
- IPR003593: AAA+ ATPase domain (domain) [458-975]
- IPR021133: HEAT, type 2 (repeat) [174-212]
- IPR015688: Elongation Factor 3, ABC2 domain, chromodomain-like insertion (domain) [799-866]
- IPR040533: Elongation factor 3, four helical bundle domain (domain) [339-414]
- IPR003439: ABC transporter-like, ATP-binding domain (domain) [429-998]
- IPR017871: ABC transporter-like, conserved site (conserved_site) [549-916]
- IPR047038: Elongation Factor 3, chromodomain-like insertion superfamily (homologous_superfamily) [767-876]
- IPR050611: ABCF protein (family) [649-980]
Interaction partners: - Elf1p
- Large ribosomal subunit protein uL18
- Cam1-1p
- Translation elongation factor EF1B gamma
- Tef4p
- Small ribosomal subunit protein uS4
- Large ribosomal subunit protein uL3
- Small ribosomal subunit protein uS5
- Small ribosomal subunit protein uS4m
- Small ribosomal subunit protein uS3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0045182 (translation regulator activity), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0016887 (ATP hydrolysis activity), GO:0097159 (organic cyclic compound binding), GO:0090079 (translation regulator activity, nucleic acid binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0003676 (nucleic acid binding), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:0008135 (translation factor activity, RNA binding), GO:0003746 (translation elongation factor activity), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0016462 (pyrophosphatase activity), GO:0017111 (ribonucleoside triphosphate phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044238 (primary metabolic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0019538 (protein metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006412 (translation), GO:0009059 (macromolecule biosynthetic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0006518 (peptide metabolic process), GO:0010467 (gene expression), GO:0043043 (peptide biosynthetic process), GO:0002181 (cytoplasmic translation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0022626 (cytosolic ribosome), GO:0043228 (non-membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005840 (ribosome)