Protein: Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) (Long-chain acyl-CoA synthetase) (Acyl-CoA synthetase) (P69451)
Organism: Escherichia coli (strain K12)
Function: Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. Activity is the highest with fatty acid substrates of > 10 carbon atoms. Is involved in the aerobic beta-oxidative degradation of fatty acids, which allows aerobic growth of E.coli on fatty acids as a sole carbon and energy source.
Subcellular location: Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Note=Partially membrane-associated. {ECO:0000250}.
Sequence: MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVNMGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVVNVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAKGTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELGGQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGGMPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLVDDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSLTEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA
InterPro domains: - IPR042099: ANL, N-terminal domain (homologous_superfamily) [11-455]
- IPR000873: AMP-dependent synthetase/ligase domain (domain) [29-419]
- IPR050237: ATP-dependent AMP-binding enzyme (family) [15-549]
- IPR045851: AMP-binding enzyme, C-terminal domain superfamily (homologous_superfamily) [456-557]
- IPR025110: AMP-binding enzyme, C-terminal domain (domain) [469-543]
- IPR020845: AMP-binding, conserved site (conserved_site) [211-222]
Interaction partners: - Acyl-coenzyme A dehydrogenase
- Fatty acid oxidation complex subunit alpha
- Long-chain fatty acid transport protein
- Fatty acid oxidation complex subunit alpha
- 3-ketoacyl-CoA thiolase FadA
- Thioesterase 1/protease 1/lysophospholipase L1
- Fatty acid metabolism regulator protein
- Malonyl CoA-acyl carrier protein transacylase
- Glycerol-3-phosphate acyltransferase
- 2,4-dienoyl-CoA reductase [
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0016877 (ligase activity, forming carbon-sulfur bonds), GO:0016878 (acid-thiol ligase activity), GO:0016405 (CoA-ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0006629 (lipid metabolic process), GO:0044282 (small molecule catabolic process), GO:0006082 (organic acid metabolic process), GO:0044248 (cellular catabolic process), GO:0044255 (cellular lipid metabolic process), GO:1901575 (organic substance catabolic process), GO:0006631 (fatty acid metabolic process), GO:0016054 (organic acid catabolic process), GO:0043436 (oxoacid metabolic process), GO:0030258 (lipid modification), GO:0044242 (cellular lipid catabolic process), GO:0016042 (lipid catabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0019395 (fatty acid oxidation), GO:0046395 (carboxylic acid catabolic process), GO:0034440 (lipid oxidation), GO:0009062 (fatty acid catabolic process), GO:0006635 (fatty acid beta-oxidation), GO:0032787 (monocarboxylic acid metabolic process), GO:0072329 (monocarboxylic acid catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)