Protein: Didemethylasterriquinone D synthetase tdiA (A7XRY0)
Organism: Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
Function: Didemethylasterriquinone D synthetase; part of the gene cluster that mediates the biosynthesis of terrequinone A, an antitumor agent. The first step in the biosynthetic pathway for terrequinone A is formation of indole pyruvic acid (IPA) from L- tryptophan by the aminotransferase tdiD. The nonribosomal peptide synthase tdiA then immediately converts unstable IPA to didemethylasterriquinone D (DDAQ D), via condensation of 2 IPA molecules. The symmetric connectivity of the 2 IPA molecules is thought to arise by head-to-tail dual Claisen condensations facilitated by the TE domain. TdiB then catalyzes reverse prenylation by transferring dimethylallyl diphosphate to carbon atom 2' of DDAQ D, to yield asterriquinone C-1. Finally, tdiC and tdiE enzymes robustly convert asterriquinone C-1 to terrequinone A via a transformation involving regular prenylation at carbon atom 5, which requires elimination of the hydroxy group on C-5
Sequence: MAPSKTEIAPLRAAKYPFGNIVDALRHAAAHTDEGIIVYHPNSISTSSPPQTVSYKDLLHQAEANATRLLQQKLCSPKSIVLVHFESALDSIVWYWSVLLAGGIPALTGPGMFSQNPADRERHLRHLSETLNSPVCLTRPALLAPFEEQTADDRIKARTVDEILAAPEIADVADAPLPALTPSSTDMLALMLTSGSSGNAKAVPLTHQQLLAAFRGKSTAASLRFPRSPFLSWVHMDHVANLVHCHIFAIVSGISQIQVPAPDLLINPAQLLNLISRHRVSRTFMPNFLCAKLRRQLESGSPEYILDPGLNLETLYIDTGGEANVTEVCIALQSLLSRYGAPDNVFKPSFGMTETVAGCIFNSHCPSYDHAQRHEFACLGKPMPGVRMRVTRLDTPSEEAAPGERGSLEVTGEVVFKGYYNNPAATAEAFTSDGWFRTGDLAFIDSNGNLHLDGRTKEMININGVKYLPYELDAALEQAQIPGATPSYFCTFSSRDATMDTEVVVVLYLPSYVESDDEARFSTQSSIIRVVAMHTRSRPRVVPLRPQDMPKSTLGKLSRAKLKTALEEGQFATQQQINDEAIRRYQQKTRASPETPDEAVILDIIKEQLEIRSDDDSFGVNDSILSIGATSMDLVAIIHRINKCLQPSQPLRLTDILKDSTARGLAVALATGAAPRSQDQSSTHVYDPVVTLQPHGTKSPLWLVHPGVGEVLVFVNLAHHITDRPVYAFRAKGFNAAAGLPETPFTSLEELFTTYRDAIKERQPHGPYAIAGYSFGGMVAFEVSKLLEQDGDEVRYCGSWNLPPHIKFRMRELVWEECVIHLFYFVGLMTELAAYTHKPTLQEFNRANRRLDAIRYLRQHCDAARWDELGLSEEYYLLWVGLASNMQSLAVDYEPSGSVKCMDVFVADPLSHVAKDRIDWVEGRLSAWKEFVREDVRFHDVQGAHYTMLNREYVEAFAGTLKNVLRERGL
InterPro domains: - IPR001031: Thioesterase (domain) [700-962]
- IPR020845: AMP-binding, conserved site (conserved_site) [190-201]
- IPR045851: AMP-binding enzyme, C-terminal domain superfamily (homologous_superfamily) [457-589]
- IPR042099: ANL, N-terminal domain (homologous_superfamily) [11-455]
- IPR009081: Phosphopantetheine binding ACP domain (domain) [595-673]
- IPR029058: Alpha/Beta hydrolase fold (homologous_superfamily) [679-965]
- IPR036736: ACP-like superfamily (homologous_superfamily) [590-679]
- IPR000873: AMP-dependent synthetase/ligase domain (domain) [39-420]
Interaction partners: - Indole prenyltransferase tdiB
- Aminotransferase tdiD
- Nucleoporin NSP1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0016877 (ligase activity, forming carbon-sulfur bonds), GO:0016878 (acid-thiol ligase activity), GO:0016405 (CoA-ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:0044249 (cellular biosynthetic process), GO:1901360 (organic cyclic compound metabolic process), GO:0018904 (ether metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006790 (sulfur compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006082 (organic acid metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0072339 (lactam biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0044272 (sulfur compound biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0019752 (carboxylic acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0072330 (monocarboxylic acid biosynthetic process), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)