Protein: SPI-2 type 3 secretion system ATPase (T3SS-2 ATPase) (EC 7.4.2.8) (P74857)
Organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Function: ATPase component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells. Acts as a molecular motor to provide the energy that is required for the export of proteins (By similarity). Required for type III secretion apparatus (T3SA) formation, secretion of a subset of SPI-2 effectors and virulence. May play a critical role in T3SS substrate recognition, disassembly of the effector/chaperone complex and unfolding of the effector in an ATP-dependent manner prior to secretion (Probable). Releases the effector protein SseB from the T3SS-2 specific chaperone SsaE in an ATP-dependent manner.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:24722491}. Note=Can associate with the membrane regardless of the presence of the other ATPase-associated components. {ECO:0000269|PubMed:24722491}.
Sequence: MKNELMQRLRLKYPPPDGYCRWGRIQDVSATLLNAWLPGVFMGELCCIKPGEELAEVVGINGSKALLSPFTSTIGLHCGQQVMALRRRHQVPVGEALLGRVIDGFGRPLDGRELPDVCWKDYDAMPPPAMVRQPITQPLMTGIRAIDSVATCGEGQRVGIFSAPGVGKSTLLAMLCNAPDADSNVLVLIGERGREVREFIDFTLSEETRKRCVIVVATSDRPALERVRALFVATTIAEFFRDNGKRVVLLADSLTRYARAAREIALAAGETAVSGEYPPGVFSALPRLLERTGMGEKGSITAFYTVLVEGDDMNEPLADEVRSLLDGHIVLSRRLAERGHYPAIDVLATLSRVFPVVTSHEHRQLAAILRRCLALYQEVELLIRIGEYQRGVDTDTDKAIDTYPDICTFLRQSKDEVCGPELLIEKLHQILTE
InterPro domains: - IPR050053: ATPase alpha/beta chains (family) [24-413]
- IPR013380: Type 3 secretion system ATPase SctN (family) [19-431]
- IPR003593: AAA+ ATPase domain (domain) [154-335]
- IPR005714: ATPase, type III secretion system, FliI/YscN (family) [13-431]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [89-357]
- IPR000194: ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain (domain) [142-351]
- IPR040627: T3SS EscN ATPase, C-terminal (domain) [358-429]
Interaction partners: - Secretion system apparatus protein SsaV
- Secretion system apparatus protein SsaQ
- Secretion system apparatus lipoprotein SsaJ
- Secretion system apparatus protein SsaT
- Virulence protein YscR
- SPI-2 type 3 secretion system stator protein
- Secretion system apparatus protein SsaL
- Secretion system apparatus protein SsaO
- Secretion system apparatus protein SsaU
- Flagellar biosynthesis protein FlhA
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0051179 (localization), GO:0042592 (homeostatic process), GO:0051234 (establishment of localization), GO:0048878 (chemical homeostasis), GO:0019725 (cellular homeostasis), GO:0055085 (transmembrane transport), GO:0050801 (monoatomic ion homeostasis), GO:0055082 (intracellular chemical homeostasis), GO:0098771 (inorganic ion homeostasis), GO:0034220 (monoatomic ion transmembrane transport), GO:0098660 (inorganic ion transmembrane transport), GO:0006810 (transport), GO:0098655 (monoatomic cation transmembrane transport), GO:0098662 (inorganic cation transmembrane transport), GO:0055080 (monoatomic cation homeostasis), GO:0006873 (intracellular monoatomic ion homeostasis), GO:0006811 (monoatomic ion transport), GO:0055074 (calcium ion homeostasis), GO:0006874 (intracellular calcium ion homeostasis), GO:0006812 (monoatomic cation transport), GO:0030003 (intracellular monoatomic cation homeostasis), GO:0030001 (metal ion transport), GO:0000041 (transition metal ion transport), GO:0006826 (iron ion transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)