Protein: Probable N-succinyldiaminopimelate aminotransferase DapC (DAP-AT) (EC 2.6.1.17) (P9WPZ5)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Involved in the lysine biosynthetic pathways. It catalyzes the transfer of an amino group from L-glutamate to N-succinyl-2-l-amino-6-oxoheptanedioate (N-succinyl-2-l-amino-6-ketopimelate) in a PLP-dependent reaction, yielding as products N-succinyl-l-2,6-diaminoheptanedioate (N-succinyl-diaminopimelate) and 2-oxoglutarate (Probable).
Subcellular location: Cytoplasm {ECO:0000305}.
Sequence: MTVSRLRPYATTVFAEMSALATRIGAVNLGQGFPDEDGPPKMLQAAQDAIAGGVNQYPPGPGSAPLRRAIAAQRRRHFGVDYDPETEVLVTVGATEAIAAAVLGLVEPGSEVLLIEPFYDSYSPVVAMAGAHRVTVPLVPDGRGFALDADALRRAVTPRTRALIINSPHNPTGAVLSATELAAIAEIAVAANLVVITDEVYEHLVFDHARHLPLAGFDGMAERTITISSAAKMFNCTGWKIGWACGPAELIAGVRAAKQYLSYVGGAPFQPAVALALDTEDAWVAALRNSLRARRDRLAAGLTEIGFAVHDSYGTYFLCADPRPLGYDDSTEFCAALPEKVGVAAIPMSAFCDPAAGQASQQADVWNHLVRFTFCKRDDTLDEAIRRLSVLAERPAT
InterPro domains: - IPR015421: Pyridoxal phosphate-dependent transferase, major domain (homologous_superfamily) [39-280]
- IPR004839: Aminotransferase, class I/classII, large domain (domain) [27-388]
- IPR015424: Pyridoxal phosphate-dependent transferase (homologous_superfamily) [4-389]
- IPR015422: Pyridoxal phosphate-dependent transferase, small domain (homologous_superfamily) [13-388]
- IPR051326: Kynurenine--oxoglutarate transaminase (family) [4-392]
Interaction partners: - Putative succinyl-diaminopimelate desuccinylase DapE
- 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
- Acetylornithine aminotransferase
- Diaminopimelate epimerase
- Aspartokinase
- Aspartate-semialdehyde dehydrogenase
- Diaminopimelate decarboxylase
- 4-hydroxy-tetrahydrodipicolinate synthase
- Putative acyltransferase Rv0859
- Probable fatty oxidation protein FadB
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0016740 (transferase activity), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0019842 (vitamin binding), GO:0043168 (anion binding), GO:0042802 (identical protein binding), GO:0016769 (transferase activity, transferring nitrogenous groups), GO:0070279 (vitamin B6 binding), GO:0008483 (transaminase activity), GO:0030170 (pyridoxal phosphate binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0043094 (cellular metabolic compound salvage), GO:0006790 (sulfur compound metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0000096 (sulfur amino acid metabolic process), GO:0044272 (sulfur compound biosynthetic process), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0043102 (amino acid salvage), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0071267 (L-methionine salvage), GO:1901607 (alpha-amino acid biosynthetic process), GO:0009066 (aspartate family amino acid metabolic process), GO:0000097 (sulfur amino acid biosynthetic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0006555 (methionine metabolic process), GO:0009086 (methionine biosynthetic process), GO:0009067 (aspartate family amino acid biosynthetic process), GO:0071265 (L-methionine biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)