Protein: Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease (ESCRT-I complex subunit VPS23) (Vacuolar protein sorting-associated protein 23) (P25604)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association to the ESCRT-0 complex. Required for vacuolar targeting of temperature-sensitive plasma membrane proteins STE2 and CAN1.
Subcellular location: Cytoplasm. Endosome. Late endosome membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
Sequence: MSANGKISVPEAVVNWLFKVIQPIYNDGRTTFHDSLALLDNFHSLRPRTRVFTHSDGTPQLLLSIYGTISTGEDGSSPHSIPVIMWVPSMYPVKPPFISINLENFDMNTISSSLPIQEYIDSNGWIALPILHCWDPAAMNLIMVVQELMSLLHEPPQDQAPSLPPKPNTQLQQEQNTPPLPPKPKSPHLKPPLPPPPPPQPASNALDLMDMDNTDISPTNHHEMLQNLQTVVNELYREDVDYVADKILTRQTVMQESIARFHEIIAIDKNHLRAVEQAIEQTMHSLNAQIDVLTANRAKVQQFSSTSHVDDEDVNSIAVAKTDGLNQLYNLVAQDYALTDTIECLSRMLHRGTIPLDTFVKQGRELARQQFLVRWHIQRITSPLS
InterPro domains: - IPR052070: ESCRT-I complex UEV domain-containing protein (family) [10-381]
- IPR017916: Steadiness box (SB) domain (domain) [317-385]
- IPR008883: Ubiquitin E2 variant, N-terminal (domain) [12-161]
- IPR016135: Ubiquitin-conjugating enzyme/RWD-like (homologous_superfamily) [1-161]
- IPR037202: ESCRT assembly domain (homologous_superfamily) [325-382]
Interaction partners: - Vacuolar protein sorting-associated protein 28
- Vacuolar protein-sorting-associated protein 36
- Protein SRN2
- Vacuolar protein sorting-associated protein 27
- Multivesicular body sorting factor 12
- Vacuolar-sorting protein SNF7
- Vacuolar protein sorting-associated protein 20
- Vacuolar protein-sorting-associated protein 25
- Vacuolar-sorting protein SNF8
- Polyubiquitin
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0006807 (nitrogen compound metabolic process), GO:0051234 (establishment of localization), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0033036 (macromolecule localization), GO:0044237 (cellular metabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044248 (cellular catabolic process), GO:0019538 (protein metabolic process), GO:0051649 (establishment of localization in cell), GO:0043170 (macromolecule metabolic process), GO:0045184 (establishment of protein localization), GO:0046907 (intracellular transport), GO:0006810 (transport), GO:0070727 (cellular macromolecule localization), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0071705 (nitrogen compound transport), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0072594 (establishment of protein localization to organelle), GO:0030163 (protein catabolic process), GO:0071702 (organic substance transport), GO:0009057 (macromolecule catabolic process), GO:0006886 (intracellular protein transport), GO:0044265 (cellular macromolecule catabolic process), GO:0015031 (protein transport), GO:0008104 (protein localization), GO:0007034 (vacuolar transport), GO:0033365 (protein localization to organelle), GO:0072666 (establishment of protein localization to vacuole), GO:0043328 (protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway), GO:0071985 (multivesicular body sorting pathway), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0045324 (late endosome to vacuole transport), GO:0051603 (proteolysis involved in protein catabolic process), GO:0015931 (nucleobase-containing compound transport), GO:0006605 (protein targeting), GO:0006623 (protein targeting to vacuole), GO:0019941 (modification-dependent protein catabolic process), GO:0072665 (protein localization to vacuole), GO:0006511 (ubiquitin-dependent protein catabolic process), GO:0043162 (ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0036452 (ESCRT complex), GO:0098796 (membrane protein complex), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0010008 (endosome membrane), GO:0098588 (bounding membrane of organelle), GO:0031982 (vesicle), GO:0012506 (vesicle membrane), GO:0030659 (cytoplasmic vesicle membrane), GO:0097708 (intracellular vesicle)