Protein: Actin-related protein 2-B (Actin-like protein 2-B) (Q56A35)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (By similarity). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (By similarity). Seems to contact the pointed end of the daughter actin filament (By similarity). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (By similarity). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (By similarity).
Subcellular location: Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q7ZTP2}. Cell projection {ECO:0000250|UniProtKB:Q7ZTP2}. Nucleus {ECO:0000250|UniProtKB:Q7ZTP2}.
Sequence: MDSQGRKVVVCDNGTGFVKCGYAGSNFPEHIFPALVGRPIIRSTAKVGNIEIKDLMVGDEASELRSMLEVNYPMENGIVRNWDDMKHLWDYTFGPEKLNINSRDCKILLTEPPMNPTKNREKIIEVMFETYQFTGVYIAIQAVLTLYAQGLLTGVVVDSGDGVTHICPVYEGFSLPHLTRRLDIAGRDITRYLIKLLLLRGYAFNHSADFETVRMMKENLCYVGYNIEQEQKLALETTVLVESYTLPDGRVIKVGGERFEAPEALFQPHLINVEGVGVAELLFNTIQAADIDTRAEFYKHIVLSGGSTMYPGLPSRLERELKQLYLERVLKGDVDKLSKFKIRIEDPPRRKHMVFLGGAVLADIMKDKDNFWLTREEYQEKGVRVLEKLGVTVR
InterPro domains: - IPR043129: ATPase, nucleotide binding domain (homologous_superfamily) [6-387]
- IPR004000: Actin family (family) [6-390]
- IPR020902: Actin/actin-like conserved site (conserved_site) [108-120]
Interaction partners: - WASP actin nucleation-promoting factor b.
- Actin nucleation-promoting factor WAS
- Arp2/3 complex 34 kDa subunit
- Actin-related protein 2/3 complex subunit 3
- Actin-related protein 3B
- Actin-related protein 3
- Hematopoietic cell-specific Lyn substrate 1
- Actin-related protein 2/3 complex subunit
- Actin, cytoplasmic 2
- Actin, cytoplasmic 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0048870 (cell motility), GO:0090130 (tissue migration), GO:0071840 (cellular component organization or biogenesis), GO:0030029 (actin filament-based process), GO:0048729 (tissue morphogenesis), GO:0048598 (embryonic morphogenesis), GO:0009888 (tissue development), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0090132 (epithelium migration), GO:0009790 (embryo development), GO:0016043 (cellular component organization), GO:0030036 (actin cytoskeleton organization), GO:0016477 (cell migration), GO:0010631 (epithelial cell migration), GO:0007369 (gastrulation), GO:0002009 (morphogenesis of an epithelium), GO:0060429 (epithelium development), GO:0006996 (organelle organization), GO:0097435 (supramolecular fiber organization), GO:0001667 (ameboidal-type cell migration), GO:0007015 (actin filament organization), GO:0007010 (cytoskeleton organization), GO:0045010 (actin nucleation), GO:0034314 (Arp2/3 complex-mediated actin nucleation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)