Protein: Neutrophil cytosol factor 1 (NCF-1) (47 kDa neutrophil oxidase factor) (NCF-47K) (Neutrophil NADPH oxidase factor 1) (p47-phox) (Q09014)
Organism: Mus musculus (Mouse)
Function: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:26021615}. Membrane {ECO:0000269|PubMed:26021615}; Peripheral membrane protein {ECO:0000250|UniProtKB:P14598}; Cytoplasmic side {ECO:0000250|UniProtKB:P14598}.
Sequence: MGDTFIRHIALLGFEKRFIPSQHYVYMFLVKWQDLSEKVVYRKFTEIYEFHKMLKEMFPIEAGEIHTENRVIPHLPAPRWFDGQRAAESRQGTLTEYFNGLMGLPVKISRCPHLLDFFKVRPDDLKLPTDSQAKKPETYLVPKDGKNNVADITGPIILQTYRAIADYEKSSGTEMTVATGDVVDVVEKSESGWWFCQMKTKRGWVPASYLEPLDSPDEAEDPDPNYAGEPYVTIKAYAAVEEDEMSLSEGEAIEVIHKLLDGWWVVRKGDITGYFPSMYLQKAGEEITQAQRQIRGRGAPPRRSTIRNAQSIHQRSRKRLSQDTYRRNSVRFLQQRRRPGRPGPLSTDGTKDNPSTPRVKPQPAVPPRPSSDLILHRCTESTKRKLTSAV
InterPro domains: - IPR015039: Neutrophil cytosol factor 1, C-terminal (domain) [359-390]
- IPR036028: SH3-like domain superfamily (homologous_superfamily) [155-332]
- IPR001655: Neutrophil cytosol factor 1 (family) [7-385]
- IPR035756: Neutrophil cytosol factor 1, first SH3 domain (domain) [160-212]
- IPR034909: Neutrophil cytosol factor 1, PX domain (domain) [6-123]
- IPR001452: SH3 domain (domain) [156-285]
- IPR035757: Neutrophil cytosol factor 1, second SH3 domain (domain) [230-282]
- IPR032136: Neutrophil cytosol factor 1, PBR/AIR (domain) [291-338]
- IPR001683: Phox homology (domain) [4-125]
- IPR051228: NADPH Oxidase and PX Domain-Containing (family) [3-386]
- IPR036871: PX domain superfamily (homologous_superfamily) [1-141]
Interaction partners: - NADPH oxidase 2
- Ras-related C3 botulinum toxin substrate 2
- NADPH oxidase activator 1
- Neutrophil cytosol factor 2
- NADPH oxidase 1
- Neutrophil cytosol factor 4
- NADPH oxidase 4
- Cytochrome b-245 light chain
- Ras-related C3 botulinum toxin substrate 1
- NADPH oxidase organizer 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0030234 (enzyme regulator activity), GO:0140677 (molecular function activator activity), GO:0008047 (enzyme activator activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0009058 (biosynthetic process), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0033036 (macromolecule localization), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0006979 (response to oxidative stress), GO:0072593 (reactive oxygen species metabolic process), GO:0019538 (protein metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901700 (response to oxygen-containing compound), GO:0051668 (localization within membrane), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0009891 (positive regulation of biosynthetic process), GO:0045184 (establishment of protein localization), GO:0010035 (response to inorganic substance), GO:0031325 (positive regulation of cellular metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0006810 (transport), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0034599 (cellular response to oxidative stress), GO:0010038 (response to metal ion), GO:0071705 (nitrogen compound transport), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0062197 (cellular response to chemical stress), GO:0071702 (organic substance transport), GO:0010468 (regulation of gene expression), GO:0090150 (establishment of protein localization to membrane), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0051592 (response to calcium ion), GO:0006886 (intracellular protein transport), GO:0015031 (protein transport), GO:0031326 (regulation of cellular biosynthetic process), GO:0051247 (positive regulation of protein metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0008104 (protein localization), GO:0051252 (regulation of RNA metabolic process), GO:0042157 (lipoprotein metabolic process), GO:1901701 (cellular response to oxygen-containing compound), GO:0000302 (response to reactive oxygen species), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0072657 (protein localization to membrane), GO:0031399 (regulation of protein modification process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0043542 (endothelial cell migration), GO:0071248 (cellular response to metal ion), GO:0010039 (response to iron ion), GO:1902680 (positive regulation of RNA biosynthetic process), GO:0034614 (cellular response to reactive oxygen species), GO:0070301 (cellular response to hydrogen peroxide), GO:0031401 (positive regulation of protein modification process), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045893 (positive regulation of DNA-templated transcription), GO:0031056 (regulation of histone modification), GO:1903320 (regulation of protein modification by small protein conjugation or removal), GO:1903322 (positive regulation of protein modification by small protein conjugation or removal), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0045944 (positive regulation of transcription by RNA polymerase II), GO:0031396 (regulation of protein ubiquitination), GO:0031398 (positive regulation of protein ubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0044297 (cell body), GO:0005829 (cytosol), GO:1902494 (catalytic complex), GO:0036477 (somatodendritic compartment), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0098796 (membrane protein complex), GO:0012505 (endomembrane system), GO:0098797 (plasma membrane protein complex), GO:0043025 (neuronal cell body), GO:0005794 (Golgi apparatus), GO:0005783 (endoplasmic reticulum), GO:0120025 (plasma membrane bounded cell projection), GO:0097447 (dendritic tree), GO:0043229 (intracellular organelle), GO:1990204 (oxidoreductase complex), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0043005 (neuron projection), GO:0005791 (rough endoplasmic reticulum), GO:0043231 (intracellular membrane-bounded organelle), GO:0030425 (dendrite)