Protein: Protein TONSOKU (Protein BRUSHY 1) (Protein MGOUN 3) (Q6Q4D0)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Histone reader involved in homologous recombination-mediated repair of double-strand breaks (DSBs) at stalled or collapsed replication forks. Specifically recognizes and binds histone H3.1 lacking methylation at 'Lys-27' (H3K27me1). Involved in structural and functional stabilization of chromatin; represents a link between response to DNA damage and epigenetic gene silencing. Required for cell arrangement in root and shoot apical meristems. May be involved, when interacting with TSA1, in the organization of spindle microtubules.
Subcellular location: Nucleus, nucleoplasm {ECO:0000269|PubMed:15082530, ECO:0000269|PubMed:15125773, ECO:0000269|PubMed:15746155, ECO:0000269|PubMed:15964904, ECO:0000269|PubMed:21705754}. Chromosome {ECO:0000269|PubMed:35298257}. Note=During anaphase, relocalizes in spots close to the ends of spindle microtubules ahead of separating chromatids.
Sequence: MGRLDVAAAKRAYRKAEEVGDRREQARWANNVGDILKNHGEYVDALKWFRIDYDISVKYLPGKDLLPTCQSLGEIYLRLENFEEALIYQKKHLQLAEEANDTVEKQRACTQLGRTYHEMFLKSEDDCEAIQSAKKYFKKAMELAQILKEKPPPGESSGFLEEYINAHNNIGMLDLDLDNPEAARTILKKGLQICDEEEVREYDAARSRLHHNLGNVFMALRSWDEAKKHIEMDINICHKINHVQGEAKGYINLAELHNKTQKYIDALLCYGKASSLAKSMQDESALVEQIEHNTKIVKKSMKVMEELREEELMLKKLSAEMTDAKGTSEERKSMLQVNACLGSLIDKSSMVFAWLKHLQYSKRKKKISDELCDKEKLSDAFMIVGESYQNLRNFRKSLKWFIRSYEGHEAIGNLEGQALAKINIGNGLDCIGEWTGALQAYEEGYRIALKANLPSIQLSALEDIHYIHMMRFGNAQKASELKETIQNLKESEHAEKAECSTQDECSETDSEGHANVSNDRPNACSSPQTPNSLRSERLADLDEANDDVPLISFLQPGKRLFKRKQVSGKQDADTDQTKKDFSVVADSQQTVAGRKRIRVILSDDESETEYELGCPKDSSHKVLRQNEEVSEESMYFDGAINYTDNRAIQDNVEEGSCSYTPLHPIKVAPNVSNCRSLSNNIAVETTGRRKKGSQCDVGDSNGTSCKTGAALVNFHAYSKTEDRKIKIEIENEHIALDSCSHDDESVKVELTCLYYLQLPDDEKSKGLLPIIHHLEYGGRVLKPLELYAILRDSSENVVIEASVDGWVHKRLMKLYMDCCQSLSEKPSMKLLKKLYISEVEDDINVSECELQDISAAPLLCALHVHNIAMLDLSHNMLGNGTMEKLKQLFASSSQMYGALTLDLHCNRFGPTALFQICECPVLFTRLEVLNVSRNRLTDACGSYLSTIVKNCRALYSLNVEHCSLTSRTIQKVANALDSKSGLSQLCIGYNNPVSGSSIQNLLAKLATLSSFAELSMNGIKLSSQVVDSLYALVKTPSLSKLLVGSSGIGTDGAIKVTESLCYQKEETVKLDLSCCGLASSFFIKLNQDVTLTSSILEFNVGGNPITEEGISALGELLRNPCSNIKVLILSKCHLKLAGLLCIIQALSDNKNLEELNLSDNAKIEDETVFGQPVKERSVMVEQEHGTCKSVTSMDKEQELCETNMECDDLEVADSEDEQIEEGTATSSSLSLPRKNHIVKELSTALSMANQLKILDLSNNGFSVEALETLYMSWSSSSSRTGIAQRHVKEETVHFYVEGKMCCGVKSCCRKD
InterPro domains: - IPR011990: Tetratricopeptide-like helical domain superfamily (homologous_superfamily) [5-519]
- IPR032675: Leucine-rich repeat domain superfamily (homologous_superfamily) [810-1282]
- IPR044227: Protein TONSOKU (family) [5-1311]
- IPR001611: Leucine-rich repeat (repeat) [1149-1166]
- IPR019734: Tetratricopeptide repeat (repeat) [26-451]
Interaction partners: - Histone H3-like centromeric protein CENH3
- Histone H3-like 4
- Histone H3-like 1
- Histone H3-like 3
- Histone H3.3
- Histone H3-like 2
- Histone H3.1
- Histone H3-like 5
- Uncharacterized protein At4g12070/F16J13_140
- Myosin heavy chain-related protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0042393 (histone binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0034470 (ncRNA processing), GO:0034660 (ncRNA metabolic process), GO:0006399 (tRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)