Protein: Arabinose 5-phosphate isomerase GutQ (API) (G-API) (EC 5.3.1.13) (Phosphosugar aldol-ketol isomerase) (P17115)
Organism: Escherichia coli (strain K12)
Function: Catalyzes the reversible aldol-ketol isomerization between D-ribulose 5-phosphate (Ru5P) and D-arabinose 5-phosphate (A5P). It appears that the physiological function of G-API may be to synthesize the regulatory molecule A5P, which in turn participates in the induction of the gut operon through an unknown mechanism. It is also able of sustaining the biosynthetic pathway of 3-deoxy-D-manno-octulosonate (KDO), a unique 8-carbon sugar component of lipopolysaccharides (LPSs).
Sequence: MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGKVVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGAKELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNTLMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASVMDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTLQSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII
InterPro domains: - IPR001347: SIS domain (domain) [34-177]
- IPR004800: Phosphosugar isomerase, KdsD/KpsF-type (family) [1-321]
- IPR046348: SIS domain superfamily (homologous_superfamily) [10-255]
- IPR046342: CBS domain superfamily (homologous_superfamily) [194-318]
- IPR050986: SIS family GutQ/KpsF subfamily isomerases (family) [3-320]
- IPR000644: CBS domain (domain) [202-321]
- IPR035474: KpsF-like, SIS domain (domain) [43-170]
Interaction partners: - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC
- Glucitol operon activator protein
- Glucitol operon repressor
- 2-dehydro-3-deoxyphosphooctonate aldolase
- 3-deoxy-manno-octulosonate cytidylyltransferase
- Tetraacyldisaccharide 4'-kinase
- 3-deoxy-D-manno-octulosonic acid transferase
- UDP-3-O-acyl-N-acetylglucosamine deacetylase
- Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
- UDP-3-O-
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016853 (isomerase activity), GO:0005515 (protein binding), GO:0042802 (identical protein binding), GO:0016860 (intramolecular oxidoreductase activity), GO:0016861 (intramolecular oxidoreductase activity, interconverting aldoses and ketoses)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044085 (cellular component biogenesis), GO:0006629 (lipid metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0016043 (cellular component organization), GO:0005996 (monosaccharide metabolic process), GO:0005975 (carbohydrate metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0046364 (monosaccharide biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0016053 (organic acid biosynthetic process), GO:0016051 (carbohydrate biosynthetic process), GO:0005976 (polysaccharide metabolic process), GO:0034637 (cellular carbohydrate biosynthetic process), GO:1903509 (liposaccharide metabolic process), GO:0022607 (cellular component assembly), GO:0034645 (cellular macromolecule biosynthetic process), GO:0043933 (protein-containing complex organization), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0008653 (lipopolysaccharide metabolic process), GO:0044264 (cellular polysaccharide metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0009103 (lipopolysaccharide biosynthetic process), GO:0033692 (cellular polysaccharide biosynthetic process), GO:0065003 (protein-containing complex assembly), GO:0000271 (polysaccharide biosynthetic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0051259 (protein complex oligomerization), GO:0051260 (protein homooligomerization), GO:0051262 (protein tetramerization), GO:0051289 (protein homotetramerization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)