Protein: Apyrase 1 (AtAPY1) (EC 3.6.1.5) (ATP-diphosphatase) (ATP-diphosphohydrolase) (Adenosine diphosphatase) (ADPase) (NTPDase) (Nucleoside triphosphate diphosphohydrolase 1) (Q9SQG2)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates. Substrate preference is ATP > ADP. Functions with APY2 to reduce extracellular ATP level which is essential for pollen germination and normal plant development. Plays a role in the regulation of stomatal function by modulating extracellular ATP levels in guard cells.
Subcellular location: Golgi apparatus membrane {ECO:0000269|PubMed:22430844}; Single-pass type II membrane protein {ECO:0000269|PubMed:22430844}. Membrane {ECO:0000305|PubMed:22430844}; Single-pass type II membrane protein {ECO:0000305|PubMed:22430844}. Note=As cell membrane protein, the functional domain could be at the extracellular side.
Sequence: MTAKRAIGRHESLADKVHRHRGLLLVISIPIVLIALVLLLMPGTSTSVSVIEYTMKNHEGGSNSRGPKNYAVIFDAGSSGSRVHVYCFDQNLDLVPLENELELFLQLKPGLSAYPNDPRQSANSLVTLLDKAEASVPRELRPKTPVRVGATAGLRALGHQASENILQAVRELLKGRSRLKTEANAVTVLDGTQEGSYQWVTINYLLRTLGKPYSDTVGVVDLGGGSVQMAYAIPEEDAATAPKPVEGEDSYVREMYLKGRKYFLYVHSYLHYGLLAARAEILKVSEDSNNPCIATGYAGTYKYGGKAFKAAASPSGASLDECRRVAINALKVNNSLCTHMKCTFGGVWNGGGGGGQKKMFVASFFFDRAAEAGFVDPNQPVAEVRPLDFEKAANKACNMRMEEGKSKFPRVEEDNLPYLCLDLVYQYTLLVDGFGLKPSQTITLVKKVKYGDYAVEAAWPLGSAIEAVSSP
InterPro domains: - IPR000407: Nucleoside phosphatase GDA1/CD39 (family) [13-470]
Interaction partners: - Probable apyrase 6
- At5g66005
- Probable apyrase 3
- Probable apyrase 5
- Probable apyrase 4
- Adenylosuccinate synthetase, chloroplastic
- At1g72880
- GMP synthase
- Apyrase 2
- Inosine triphosphate pyrophosphatase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0016462 (pyrophosphatase activity), GO:0017110 (nucleoside diphosphate phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044238 (primary metabolic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0070085 (glycosylation), GO:0044249 (cellular biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0043413 (macromolecule glycosylation), GO:1901135 (carbohydrate derivative metabolic process), GO:0019538 (protein metabolic process), GO:0036211 (protein modification process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0006486 (protein glycosylation), GO:0043412 (macromolecule modification), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0009100 (glycoprotein metabolic process), GO:0009101 (glycoprotein biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031984 (organelle subcompartment), GO:0005794 (Golgi apparatus), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043227 (membrane-bounded organelle), GO:0098791 (Golgi apparatus subcompartment), GO:0031985 (Golgi cisterna), GO:0043231 (intracellular membrane-bounded organelle), GO:0005795 (Golgi stack), GO:0031982 (vesicle), GO:0005802 (trans-Golgi network), GO:0005797 (Golgi medial cisterna), GO:0097708 (intracellular vesicle)