Protein: ATP-dependent DNA helicase Q-like 4A (EC 5.6.2.4) (DNA 3'-5' helicase RecQ4A) (RecQ-like protein 4A) (AtRecQ4A) (AtRecQl4A) (SGS1-like protein) (AtSGS1) (Q8L840)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: 3'-5' DNA helicase involved in DNA repair. Required for the maintenance of genome stability by modulation of the DNA damage response and repression of crossovers. Confers resistance to genotoxic stress. Suppresses spontaneous homologous recombination (HR) events in somatic cells together with its partners RMI1 and TOP3A. Contributes to the maintenance of chromosome integrity during meiosis. Involved in the removal of telomeric bridges that appear to arise during meiotic recombination. Required to resolve or dissolve MSH4-dependent telomeric associations. Does not seem required for chiasma formation.
Subcellular location: Nucleus {ECO:0000269|PubMed:21265901}. Chromosome {ECO:0000269|PubMed:21265901}. Note=Associates with recombination intermediates and telomeres of meiotic chromosomes. {ECO:0000269|PubMed:21265901}.
Sequence: MINSNQMSRSHLPEVQKPRGPQTNWSEHAKALESSSSVTKFLSSNVLYALESQKPRDMAARSIAFPSVNVHTLAHPQISKAWRALSSLSVNNTYLRPGVTPPIDVGTNDSYSARERSTAKVISSTGGSVYSSTRPNLSAMNVSGTGRSFHSFPSSVPGDDKIVAEKFPRGNNEIRESEPSCTHLNGVEKSFGNSAFPAEQFESRKACLDDMDDDDILENIDVDQIVMEHYHSTSTPQPSVSNFSLRTPPVDRSASRLEEECNLPPELCSNCSHGIKLGLCPEASTHVEQMKDVLLAISNELLDDATDLSPDRVGQLRQERLRLKKQIQQLENHIRDKESQKSQFLSSTATRIFQYETPKSTNYKMDQPQTDFRAHVSDQGRYACDSWNTPRDSSFSVDRYGLSSAPVEREQYVPKIIDVTYTEGSNDKKWSSREFPWTRKLEVNNKKVFGNHSFRPNQREIINATMSGSDVFVLMPTGGGKSLTYQLPALICGGITLVISPLVSLIQDQIMNLLQANIPAASLSAGMEWAEQLKIFQELNSEHSKYKLLYVTPEKVAKSDSLLRHLENLNSRGLLARFVIDEAHCVSQWGHDFRPDYQSLGILKQKFPNIPVLALTATATASVKEDVVQALGLVNCVVFRQSFNRPNLWYSVVPKTKKCLEDIDKFIKENHFDECGIIYCLSRMDCEKVSERLQEFGHKAAFYHGSMEPEQRAFIQTQWSKDEINIICATVAFGMGINKPDVRFVIHHSLPKSIEGYHQECGRAGRDGQRSSCVLYYGYGDYIRVKHMISQGGVDQSPMATGYNRVASSGRLLETNTENLLRMVRYCENEVECRRFLQLVHLGEKFDSTNCKKTCDNCCSSQSLIDKDVTLITRQLVELVKQTGERFSSAHILEVYRGSLNQMVKKHRHETLQFHGAGKHLSKIEVSRILHYLVTEDILVEDVRKSDMYGSVSSLLQVNNAKATILFSGSQTIVMKFPSSVKVLKPSKQGATAAKGPLTSEKQSTLPLTTEDAPPKDVNLSANMYTALRKLRTALVKEAPDGVMAYHIFINSTLQQISRRIPRTKEELLEINGLGKAKVSKYGDQLLETIETTVNEYYGTNKKDSIISNDSPDSGKRRRDENISPNVAEDDDFEVSPSQSCKKTVRNKSNEVLHGECIDGDRRGMELDFDFKDEDGSEIRPEGRVLPW
InterPro domains: - IPR032284: ATP-dependent DNA helicase RecQ, zinc-binding domain (domain) [779-859]
- IPR002464: DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site (conserved_site) [576-585]
- IPR002121: HRDC domain (domain) [1018-1100]
- IPR044876: HRDC domain superfamily (homologous_superfamily) [1015-1100]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [455-624]
- IPR004589: DNA helicase, ATP-dependent, RecQ type (family) [446-919]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [417-909]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [450-653]
- IPR010997: HRDC-like superfamily (homologous_superfamily) [1021-1094]
- IPR018982: RQC domain (domain) [867-980]
- IPR001650: Helicase, C-terminal domain-like (domain) [661-807]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [866-977]
Interaction partners: - DNA topoisomerase 3-alpha
- Crossover junction endonuclease MUS81
- DEAD-box ATP-dependent RNA helicase FANCM
- DNA mismatch repair protein MLH1
- Exonuclease 1
- Exonuclease 1
- DNA repair protein XRCC3 homolog
- DNA mismatch repair protein MSH5
- AT5G19950 protein
- Serine/threonine-protein kinase ATR
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0004386 (helicase activity), GO:0008094 (ATP-dependent activity, acting on DNA), GO:0005515 (protein binding), GO:0140097 (catalytic activity, acting on DNA), GO:0003678 (DNA helicase activity), GO:0043138 (3'-5' DNA helicase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0051716 (cellular response to stimulus), GO:0009628 (response to abiotic stimulus), GO:0006950 (response to stress), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0009266 (response to temperature stimulus), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009409 (response to cold), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0070417 (cellular response to cold), GO:0006259 (DNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0006310 (DNA recombination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)