Protein: 3-ketoacyl-CoA thiolase, mitochondrial (EC 2.3.1.16) (Acetyl-CoA acetyltransferase) (EC 2.3.1.9) (Acetyl-CoA acyltransferase) (Acyl-CoA hydrolase, mitochondrial) (EC 3.1.2.-, EC 3.1.2.1, EC 3.1.2.2) (Beta-ketothiolase) (Mitochondrial 3-oxoacyl-CoA thiolase) (P13437)
Organism: Rattus norvegicus (Rat)
Function: In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (Probable). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (Probable). Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies (Probable). Also displays hydrolase activity on various fatty acyl-CoAs. Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (Probable). Abolishes BNIP3-mediated apoptosis and mitochondrial damage (By similarity).
Subcellular location: Mitochondrion {ECO:0000269|PubMed:16476568}.
Sequence: MALLRGVFIVAAKRTPFGAYGGLLKDFTATDLTEFAARAALSAGKVPPETIDSVIVGNVMQSSSDAAYLARHVGLRVGVPTETGALTLNRLCGSGFQSIVSGCQEICSKDAEVVLCGGTESMSQSPYSVRNVRFGTKFGLDLKLEDTLWAGLTDQHVKLPMGMTAENLAAKYNISREDCDRYALQSQQRWKAANEAGYFNEEMAPIEVKTKKGKQTMQVDEHARPQTTLEQLQNLPPVFKKEGTVTAGNASGMSDGAGVVIIASEDAVKKHNFTPLARVVGYFVSGCDPAIMGIGPVPAITGALKKAGLSLKDMDLIDVNEAFAPQFLAVQKSLDLDPSKTNVSGGAIALGHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGISLIIQNTA
InterPro domains: - IPR020610: Thiolase, active site (active_site) [377-390]
- IPR020616: Thiolase, N-terminal (domain) [7-266]
- IPR002155: Thiolase (family) [1-397]
- IPR020613: Thiolase, conserved site (conserved_site) [342-358]
- IPR020617: Thiolase, C-terminal (domain) [274-394]
- IPR016039: Thiolase-like (homologous_superfamily) [7-394]
- IPR020615: Thiolase, acyl-enzyme intermediate active site (active_site) [88-106]
Interaction partners: - Trifunctional enzyme subunit alpha, mitochondrial
- Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial
- Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
- Hydroxymethylglutaryl-CoA synthase, mitochondrial
- Hydroxymethylglutaryl-CoA synthase, cytoplasmic
- Peroxisomal bifunctional enzyme
- Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
- Long-chain specific acyl-CoA dehydrogenase, mitochondrial
- Short-chain specific acyl-CoA dehydrogenase, mitochondrial
- Peroxisomal acyl-coenzyme A oxidase 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016746 (acyltransferase activity), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0009605 (response to external stimulus), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0042221 (response to chemical), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0044282 (small molecule catabolic process), GO:0044248 (cellular catabolic process), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0044255 (cellular lipid metabolic process), GO:1901700 (response to oxygen-containing compound), GO:0010033 (response to organic substance), GO:0009991 (response to extracellular stimulus), GO:0006629 (lipid metabolic process), GO:0042594 (response to starvation), GO:0060322 (head development), GO:0070887 (cellular response to chemical stimulus), GO:0031668 (cellular response to extracellular stimulus), GO:1901575 (organic substance catabolic process), GO:0071496 (cellular response to external stimulus), GO:0006091 (generation of precursor metabolites and energy), GO:0097305 (response to alcohol), GO:0006720 (isoprenoid metabolic process), GO:0071310 (cellular response to organic substance), GO:0048732 (gland development), GO:0061008 (hepaticobiliary system development), GO:0070542 (response to fatty acid), GO:0007420 (brain development), GO:0016054 (organic acid catabolic process), GO:0007399 (nervous system development), GO:0033993 (response to lipid), GO:0031667 (response to nutrient levels), GO:0031670 (cellular response to nutrient), GO:0007417 (central nervous system development), GO:0014070 (response to organic cyclic compound), GO:1901568 (fatty acid derivative metabolic process), GO:1901701 (cellular response to oxygen-containing compound), GO:0031669 (cellular response to nutrient levels), GO:0030182 (neuron differentiation), GO:0045471 (response to ethanol), GO:0071375 (cellular response to peptide hormone stimulus), GO:0048709 (oligodendrocyte differentiation), GO:0071398 (cellular response to fatty acid), GO:0071407 (cellular response to organic cyclic compound), GO:0071396 (cellular response to lipid), GO:0019752 (carboxylic acid metabolic process), GO:0001889 (liver development), GO:0070723 (response to cholesterol), GO:0048699 (generation of neurons), GO:0097306 (cellular response to alcohol), GO:1901569 (fatty acid derivative catabolic process), GO:0006739 (NADP metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)