Protein: Protein argonaute (H2J4R4)
Organism: Marinitoga piezophila (strain DSM 14283 / JCM 11233 / KA3)
Function: An RNA-guided ssDNA endonuclease that may play a role in defense against invading mobile genetic elements. Uses short 5'-OH- ssRNA sequences as guides (gRNA) to bind complementary target DNA (tDNA) or target RNA resulting in target cleavage. The cleavage site is 10 nucleotides (nt) downstream of the target residue base-paired with the 5'-end of the gRNA. Reaction rates are fastest on 5'-OH-gRNA:tDNA followed by 5'-OH-gRNA:target RNA. gRNA between 17-21 nt supports equivalent rates of cleavage, has no preferred 5'-nt. Has weak activity on tDNA with 5'-phospho-gRNA, yielding products 1-2 nt longer. Unlike other characterized prokaryotic Ago proteins symmetric mismatches centered around the cleavage site reduce cleavage efficiency
Subcellular location: Cytoplasm
Sequence: MYLNLYKIDIPKKIKRLYFYNPDMEPKLFARNLSRVNNFKFQDSNDLVWIEIPDIDFQITPKNVFQYKVEKEEIIKEEEDKKLFVKTLYKYIKKLFLDNDFYFKKGNNFISNSEVFSLDSNENVNAHLTYKIKIHNISNEYYLSILPKFTFLSKEPALESAIKSGYLYNIKSGKSFPYISGLDGILKIDIGNNQIVEVAYPENYLFNFTTRDAEKYGFSKEVHEIYKNKVFEGFKKIPKTLGFLNKITNLNENYQLKDGYKIFINVIYKFKNGESRYAKDVFKYSFYKNEQPLKAIFFFSSKKQFFEVQKSLKELFHNKHSVFYRAAAELGFSKVEFLRDSKTKSSAFLYNPEEFTVKNTEFINQIEDNVMAIVLLDKYIGNIDPLVRNFPDNLILQPILKEKLEDIKPFIIKSYVYKMGNFIPECKPFILKKMEDKEKNLYIGIDLSHDTYARKTNLCIAAVDNTGDILYIGKHKNLELNEKMNLDILEKEYIKAFEKYIEKFNVSPENVFILRDGRFIEDIEIIKNFISYNDTKYTLVEVNKNTNINSYDDLKEWIIKLDENTYIYYPKTFLNQKGVEVKILENNTDYTIEEIIEQIYLLTRVAHSTPYTNYKLPYPLHIANKVALTDYEWKLYIPY
InterPro domains: - IPR054434: Argonaute, middle domain, bacteria (domain) [263-432]
- IPR054387: Argonaute, N-terminal domain, bacteria (domain) [12-76]
- IPR003165: Piwi domain (domain) [394-634]
- IPR012337: Ribonuclease H-like superfamily (homologous_superfamily) [415-627]
- IPR036397: Ribonuclease H superfamily (homologous_superfamily) [436-634]
Interaction partners: - CRISPR-associated endonuclease Cas1
- CRISPR-associated endoribonuclease Cas2
- TOTE conflict system primase domain-containing protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0036260 (RNA capping), GO:0006370 (7-methylguanosine mRNA capping), GO:0009452 (7-methylguanosine RNA capping)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0018995 (host cellular component), GO:0043657 (host cell), GO:0033643 (host cell part), GO:0033646 (host intracellular part), GO:0043656 (host intracellular region), GO:0030430 (host cell cytoplasm), GO:0033655 (host cell cytoplasm part), GO:0033647 (host intracellular organelle), GO:0033648 (host intracellular membrane-bounded organelle)