Protein: Meiotic recombination protein REC8 homolog (Cohesin Rec8p) (Q6AYJ4)
Organism: Rattus norvegicus (Rat)
Function: Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity).
Subcellular location: Nucleus {ECO:0000269|PubMed:12615909}. Chromosome {ECO:0000269|PubMed:12615909}. Chromosome, centromere {ECO:0000269|PubMed:12615909}. Note=In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II. {ECO:0000269|PubMed:12615909}.
Sequence: MFYYPNVLQRHTGCFATIWLAATRGSRLVKREYLKVNVVKTCEEILNYVLVRVQPPMPGLPRPRFSLYLSAQLQIGVIRVYFQQCQYLVEDIQHILEHLHRAQLRIRIDMEEADLPSLLLPNCLAMMETLEDAPEPFFGTMSVDPTLPSPFDIPQIRHLLEAATPEKIREETLPEATPEPRKPDRTLAMVQSPEVITLQEAEPIRMLRIEGEQDLPEISRGDLDLLIAEEDDAILLEERQRGRLLRQRRASPPLDESKEEPRALEGDGLVSSLSPPAPAQVEGIREPLPDQAFPPEVQKMPGWEPGVLLTEVTPPQELRLPAPPSIEKRPPSPQRPPRRRRRGRQLLFWDKETQISREKFEEQLQTGAHCCEYPVVQPPKRKLMSPAELFRTPTLSGWLSPELLALWTHCAQVPPRMLRQRPQLETEETVEEERVADKEERRKTEALSEIEVLREAQEPSGPLMLSSELSLEVAEEEKSRTSFIPPEERWAWIEERQPEPPALPMLPELPEVPMEMPPGPELLSSEAVLRAVALELQANREPDFSSLVPPLSSRKLASRVFYLLLVLSAQKILLVDQQKPYGRLLIRLGPKFP
InterPro domains: - IPR006910: Rad21/Rec8-like protein, N-terminal (domain) [1-112]
- IPR036390: Winged helix DNA-binding domain superfamily (homologous_superfamily) [526-592]
- IPR039781: Rad21/Rec8-like protein (family) [1-592]
- IPR006909: Rad21/Rec8-like protein, C-terminal, eukaryotic (domain) [539-592]
Interaction partners: - Structural maintenance of chromosomes protein 3
- Cohesin subunit SA-3
- Structural maintenance of chromosomes protein
- separase
- Structural maintenance of chromosomes protein 1A
- Cohesin subunit SA
- RAD21 cohesin complex component like 1
- Cohesin subunit SA
- Shugoshin 2
- Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0022414 (reproductive process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0019953 (sexual reproduction), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0051321 (meiotic cell cycle), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:0071840 (cellular component organization or biogenesis), GO:0009566 (fertilization), GO:0071704 (organic substance metabolic process), GO:0032504 (multicellular organism reproduction), GO:0003006 (developmental process involved in reproduction), GO:0044237 (cellular metabolic process), GO:0022412 (cellular process involved in reproduction in multicellular organism), GO:0051716 (cellular response to stimulus), GO:0021700 (developmental maturation), GO:1903046 (meiotic cell cycle process), GO:0006950 (response to stress), GO:0048609 (multicellular organismal reproductive process), GO:0044238 (primary metabolic process), GO:0061982 (meiosis I cell cycle process), GO:0007276 (gamete generation), GO:0007281 (germ cell development), GO:0044085 (cellular component biogenesis), GO:0048468 (cell development), GO:0046483 (heterocycle metabolic process), GO:0030154 (cell differentiation), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0048515 (spermatid differentiation), GO:0070192 (chromosome organization involved in meiotic cell cycle), GO:0045132 (meiotic chromosome segregation), GO:0048608 (reproductive structure development), GO:0045137 (development of primary sexual characteristics), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0007283 (spermatogenesis), GO:0071695 (anatomical structure maturation), GO:0035295 (tube development), GO:0007059 (chromosome segregation), GO:1901360 (organic cyclic compound metabolic process), GO:0140013 (meiotic nuclear division), GO:0006139 (nucleobase-containing compound metabolic process), GO:0048469 (cell maturation), GO:0033554 (cellular response to stress), GO:0016043 (cellular component organization), GO:0009994 (oocyte differentiation), GO:0007548 (sex differentiation), GO:0001556 (oocyte maturation), GO:0046661 (male sex differentiation), GO:0044260 (cellular macromolecule metabolic process), GO:0007140 (male meiotic nuclear division), GO:0061458 (reproductive system development), GO:0098813 (nuclear chromosome segregation), GO:0007141 (male meiosis I), GO:0006974 (cellular response to DNA damage stimulus), GO:0048477 (oogenesis), GO:0008406 (gonad development), GO:0046546 (development of primary male sexual characteristics), GO:0007286 (spermatid development), GO:0007127 (meiosis I), GO:0072520 (seminiferous tubule development), GO:0022607 (cellular component assembly), GO:0045143 (homologous chromosome segregation), GO:0007130 (synaptonemal complex assembly), GO:0007292 (female gamete generation), GO:0090304 (nucleic acid metabolic process), GO:0048599 (oocyte development), GO:0006259 (DNA metabolic process), GO:0007129 (homologous chromosome pairing at meiosis), GO:0006996 (organelle organization), GO:0048232 (male gamete generation), GO:0048285 (organelle fission), GO:0006310 (DNA recombination), GO:0006281 (DNA repair), GO:0051276 (chromosome organization), GO:0008584 (male gonad development), GO:0000725 (recombinational repair), GO:0000280 (nuclear division), GO:0070193 (synaptonemal complex organization), GO:0006302 (double-strand break repair), GO:0000724 (double-strand break repair via homologous recombination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0099086 (synaptonemal structure), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0000800 (lateral element), GO:0000795 (synaptonemal complex), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0005634 (nucleus), GO:0031981 (nuclear lumen), GO:0005694 (chromosome), GO:0000228 (nuclear chromosome), GO:0000793 (condensed chromosome), GO:0000794 (condensed nuclear chromosome)