Protein: Pyruvate dehydrogenase [NADP(+)], mitochondrial (EC 1.2.1.51) (Aquacobalamin reductase [NADPH]) (EgPNOmt) (Pyruvate:NADP(+) oxidoreductase) (Q94IN5)
Organism: Euglena gracilis
Function: Pyruvate dehydrogenase [NADP(+)] is one of three enzymes participating in respiratory metabolism. The enzyme is also active with 2-oxobutyrate and oxaloacetate. The enzyme is oxygen sensitive.
Subcellular location: Mitochondrion {ECO:0000269|PubMed:11319255, ECO:0000269|PubMed:12623066}.
Sequence: MKQSVRPIISNVLRKEVALYSTIIGQDKGKEPTGRTYTSGPKPASHIEVPHHVTVPATDRTPNPDAQFFQSVDGSQATSHVAYALSDTAFIYPITPSSVMGELADVWMAQGRKNAFGQVVDVREMQSEAGAAGALHGALAAGAIATTFTASQGLLLMIPNMYKIAGELMPSVIHVAARELAGHALSIFGGHADVMAVRQTGWAMLCSHTVQQSHDMALISHVATLKSSIPFVHFFDGFRTSHEVNKIKMLPYAELKKLVPPGTMEQHWARSLNPMHPTIRGTNQSADIYFQNMESANQYYTDLAEVVQETMDEVAPYIGRHYKIFEYVGAPDAEEVTVLMGSGATTVNEAVDLLVKRGKKVGAVLVHLYRPWSTKAFEKVLPKTVKRIAALDRCKEVTALGEPLYLDVSATLNLFPERQNVKVIGGRYGLGSKDFIPEHALAIYANLASENPIQRFTVGITDDVTGTSVPFVNERVDTLPEGTRQCVFWGIGSDGTVGANRSAVRIIGDNSDLMVQAYFQFDAFKSGGVTSSHLRFGPKPITAQYLVTNADYIACHFQEYVKRFDMLDAIREGGTFVLNSRWTTEDMEKEIPADFRRNVAQKKVRFYNVDARKICDSFGLGKRINMLMQACFFKLSGVLPLAEAQRLLNESIVHEYGKKGGKVVEMNQAVVNAVFAGDLPQEVQVPAAWANAVDTSTRTPTGIEFVDKIMRPLMDFKGDQLPVSVMTPGGTFPVGTTQYAKRAIAAFIPQWIPANCTQCNYCSYVCPHATIRPFVLTDQEVQLAPESFVTRKAKGDYQGMNFRIQVAPEDCTGCQVCVETCPDDALEMTDAFTATPVQRTNWEFAIKVPNRGTMTDRYSLKGSQFQQPLLEFSGACEGCGETPYVKLLTQLFGERTVIANATGCSSIWGGTAGLAPYTTNAKGQGPAWGNSLFEDNAEFGFGIAVANAQKRSRVRDCILQAVEKKVADEGLTTLLAQWLQDWNTGDKTLKYQDQIIAGLAQQRSKDPLLEQIYGMKDMLPNISQWIIGGDGWANDIGFGGLDHVLASGQNLNVLVLDTEMYSNTGGQASKSTHMASVAKFALGGKRTNKKNLTEMAMSYGNVYVATVSHGNMAQCVKAFVEAESYDGPSLIVGYAPCIEHGLRAGMARMVQESEAAIATGYWPLYRFDPRLATEGKNPFQLDSKRIKGNLQEYLDRQNRYVNLKKNNPKGADLLKSQMADNITARFNRYRRMLEGPNTKAAAPSGNHVTILYGSETGNSEGLAKELATDFERREYSVAVQALDDIDVADLENMGFVVIAVSTCGQGQFPRNSQLFWRELQRDKPEGWLKNLKYTVFGLGDSTYYFYCHTAKQIDARLAALGAQRVVPIGFGDDGDEDMFHTGFNNWIPSVWNELKTKTPEEALFTPSIAVQLTPNATPQDFHFAKSTPVLSITGAERITPADHTRNFVTIRWKTDLSYQVGDSLGVFPENTRSVVEEFLQYYGLNPKDVITIENKGSRELPHCMAVGDLFTKVLDILGKPNNRFYKTLSYFAVDKAEKERLLKIAEMGPEYSNILSEMYHYADIFHMFPSARPTLQYLIEMIPNIKPRYYSISSAPIHTPGEVHSLVLIDTWITLSGKHRTGLTCTMLEHLQAGQVVDGCIHPTAMEFPDHEKPVVMCAMGSGLAPFVAFLRERSTLRKQGKKTGNMALYFGNRYEKTEFLMKEELKGHINDGLLTLRCAFSRDDPKKKVYVQDLIKMDEKMMYDYLVVQKGSMYCCGSRSFIKPVQESLKHCFMKAGGLTAEQAENEVIDMFTTGRYNIEAW
InterPro domains: - IPR001433: Oxidoreductase FAD/NAD(P)-binding (domain) [1657-1767]
- IPR017927: FAD-binding domain, ferredoxin reductase-type (domain) [1425-1650]
- IPR009014: Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain II (homologous_superfamily) [323-481]
- IPR002869: Pyruvate-flavodoxin oxidoreductase, central domain (homologous_superfamily) [482-735]
- IPR023173: NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamily (homologous_superfamily) [1468-1586]
- IPR001094: Flavodoxin-like (domain) [1249-1373]
- IPR037112: Pyruvate-flavodoxin oxidoreductase, EKR domain superfamily (homologous_superfamily) [697-735]
- IPR017900: 4Fe-4S ferredoxin, iron-sulphur binding, conserved site (conserved_site) [811-822]
- IPR003097: Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-binding (domain) [1430-1625]
- IPR019456: Pyruvate-flavodoxin oxidoreductase, EKR domain (domain) [690-746]
- IPR002880: Pyruvate flavodoxin/ferredoxin oxidoreductase, pyrimidine binding domain (domain) [74-311]
- IPR011895: Pyruvate-flavodoxin oxidoreductase (family) [70-1232]
- IPR017896: 4Fe-4S ferredoxin-type, iron-sulphur binding domain (domain) [747-831]
- IPR001709: Flavoprotein pyridine nucleotide cytochrome reductase (domain) [1458-1745]
- IPR029039: Flavoprotein-like superfamily (homologous_superfamily) [1242-1401]
- IPR019752: Pyruvate/ketoisovalerate oxidoreductase, catalytic domain (domain) [492-674]
- IPR029061: Thiamin diphosphate-binding fold (homologous_superfamily) [72-1273]
- IPR017938: Riboflavin synthase-like beta-barrel (homologous_superfamily) [1417-1642]
- IPR033412: Pyruvate:ferredoxin oxidoreductase, core domain II (domain) [333-427]
- IPR008254: Flavodoxin/nitric oxide synthase (domain) [1248-1391]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016903 (oxidoreductase activity, acting on the aldehyde or oxo group of donors)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006091 (generation of precursor metabolites and energy), GO:0015980 (energy derivation by oxidation of organic compounds), GO:0045333 (cellular respiration), GO:0009060 (aerobic respiration)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)