Protein: E3 ubiquitin-protein ligase Iruka (EC 2.3.2.27) (Q9VHI7)
Organism: Drosophila melanogaster (Fruit fly)
Function: E3 ubiquitin-protein ligase that mediates E2-dependent, 'Lys-48'- and/or 'Lys-63'-linked polyubiquitination of substrates. Recognizes miRNA-empty Ago1 and triggers its degradation via polyubiquitination independently of the Bag6 complex. By targeting miRNA-empty Ago1, eliminates dysfunctional Ago1 not able to bind miRNA and thereby plays a role in the quality control of miRNA-mediated silencing.
Sequence: MAEAMVVEDRPAEAKRFFCHMCNVEINIPNSDFTCPLCANGFVEELPANAPEMDSSTAGASGSARSGSSGSGSSGSHDTLSRGSSSSGSQVNVESLRNDIVSLLNMRNVPNLEITIEPNRRHSNVLHLGGFGGPSGSDSARGLTAGGRVRPANLDRLDNVLFDFLQSLPLAGATAEIVTGPGGGGVGGGGNSHMFFMGNPGDYAWGREGLDTIVTQMLNQMETSGPPPLSAQRINEIPNVQINAEEVNRKIQCSICWDDFKIDETVRKLPCSHLYHENCIVPWLNLHSTCPICRKSLADDGNDADDEFVMLDAFGPEMAADGSNSERRSASTATGTDNPSPANNPSQAAAEGGRTRPDANPAQAARNNIFTFDDDNMFLD
InterPro domains: - IPR051834: RING finger E3 ubiquitin-protein ligase (family) [56-301]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [215-299]
- IPR039525: E3 ubiquitin-protein ligase RNF126-like, zinc-ribbon (domain) [16-46]
- IPR001841: Zinc finger, RING-type (domain) [252-294]
Interaction partners: - Large proline-rich protein BAG6
- Ubiquitin-ribosomal protein eL40 fusion protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0005102 (signaling receptor binding), GO:0019787 (ubiquitin-like protein transferase activity), GO:0070851 (growth factor receptor binding), GO:0004842 (ubiquitin-protein transferase activity), GO:0061659 (ubiquitin-like protein ligase activity), GO:0061630 (ubiquitin protein ligase activity), GO:0005154 (epidermal growth factor receptor binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009628 (response to abiotic stimulus), GO:0023056 (positive regulation of signaling), GO:0042221 (response to chemical), GO:0048584 (positive regulation of response to stimulus), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0009967 (positive regulation of signal transduction), GO:0001666 (response to hypoxia), GO:1901564 (organonitrogen compound metabolic process), GO:0010646 (regulation of cell communication), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0044248 (cellular catabolic process), GO:0019538 (protein metabolic process), GO:0070482 (response to oxygen levels), GO:0009966 (regulation of signal transduction), GO:0010647 (positive regulation of cell communication), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0071456 (cellular response to hypoxia), GO:0036211 (protein modification process), GO:0009057 (macromolecule catabolic process), GO:0043412 (macromolecule modification), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0071453 (cellular response to oxygen levels), GO:0044265 (cellular macromolecule catabolic process), GO:0036293 (response to decreased oxygen levels), GO:0030163 (protein catabolic process), GO:1902533 (positive regulation of intracellular signal transduction), GO:1902531 (regulation of intracellular signal transduction), GO:0043408 (regulation of MAPK cascade), GO:0036294 (cellular response to decreased oxygen levels), GO:0043410 (positive regulation of MAPK cascade), GO:0070647 (protein modification by small protein conjugation or removal), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0010498 (proteasomal protein catabolic process), GO:0051603 (proteolysis involved in protein catabolic process), GO:0070374 (positive regulation of ERK1 and ERK2 cascade), GO:0032446 (protein modification by small protein conjugation), GO:0019941 (modification-dependent protein catabolic process), GO:0070372 (regulation of ERK1 and ERK2 cascade), GO:0006511 (ubiquitin-dependent protein catabolic process), GO:0016567 (protein ubiquitination), GO:0000209 (protein polyubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)