Protein: DNA repair protein crb2 (Checkpoint mediator protein crb2) (Cut5-repeat binding protein 2) (RAD9 protein homolog) (P87074)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Essential for cell cycle arrest at the G1 and G2 stages following DNA damage by X-, and UV-irradiation, or inactivation of DNA ligase. Plays a role in the response to DNA damage. Interaction with rad4 via its phosphorylation sites in the N-terminus couples the DNA checkpoint apparatus to chromatin via interaction of its C-terminal BRCT domains with epigenetic modifications on histones H4 and H2A, respectively, in the G1/S phase of the cell cycle, and facilitates recruitment of the checkpoint kinase chk1.
Subcellular location: Nucleus {ECO:0000269|PubMed:14655046, ECO:0000269|PubMed:15550243, ECO:0000269|PubMed:9407031}. Note=Recruited to sites of DNA damage, such as double stand breaks. {ECO:0000269|PubMed:18826944, ECO:0000269|PubMed:20679485, ECO:0000269|PubMed:24074952}.
Sequence: MEVNDTSLHKGFGLDINSQRVFGAQAAISRNNYSKVNASINPSPPRSNDNSNKEFSYSKDVNNNGAVEELSLTQLFEVPSQAAFAKQSSQDISDDELIQHDSRKVISSPYSPKQTHTVLKRLYDRQSVISDHEKLLTPQNVSNSSQILSPFTSLLPSTLSTLKDTPLSVSQNEKNLETVGEVLVPETVAQHRTKFYDYTLDEMENETESGQVETTPTRLATSLGSPVLYGRVESTPPAFLPETSEKQYKRKFSFTEPSSEKVDNTETKFSKKTKNINDENFPNPFNVISSYETSASPSTVIDQSSQVSSIFVNKRLRKSVNNQAISRSDSLSLDTPKIDSLFTRASIKPLKPSQSPNSRRSFKNRVLAFFKGYPSFYYPATLVAPVHSAVTSSIMYKVQFDDATMSTVNSNQIKRFFLKKGDVVQSTRLGKIKHTVVKTFRSTNEQLSLIAVDALNNDMVILAHGEIEVTVPISTIYVAPVNIRRFQGRDLSFSTLKDMKFEETSFLPSHDSQRNRSSLKERDSSFVKKNLDSESNQLIFDDCVFAFSGPVHEDAYDRSALETVVQDHGGLVLDTGLRPLFNDPFKSKQKKLRHLKPQKRSKSWNQAFVVSDTFSRKVKYLEALAFNIPCVHPQFIKQCLKMNRVVDFSPYLLASGYSHRLDCTLSQRIEPFDTTDSLYDRLLARKGPLFGKKILFIIPEAKSWQKKIENTEQGQKALAHVYHALALGADVEIRPNVAHLECDLILTMDGNIVDETNCPVVDPEWIVECLISQSDIST
InterPro domains: - IPR055249: Crb2, Tudor domain, schizosaccharomyces (domain) [419-491]
- IPR047249: TP53-binding protein 1-like, first BRCT domain (domain) [539-644]
- IPR047252: TP53-binding protein 1-like (family) [517-773]
- IPR041297: DNA repair protein Crb2, Tudor domain (domain) [363-416]
- IPR001357: BRCT domain (domain) [535-778]
- IPR036420: BRCT domain superfamily (homologous_superfamily) [534-778]
Interaction partners: - S-M checkpoint control protein rad4
- Serine/threonine-protein kinase chk1
- Serine/threonine-protein kinase cds1
- Protein kinase rad3
- BRCT-containing protein 1
- DNA repair protein rad9
- DNA damage checkpoint control protein rad1
- Mediator of replication checkpoint protein 1
- Checkpoint protein rad17
- Checkpoint protein hus1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0060090 (molecular adaptor activity), GO:0030674 (protein-macromolecule adaptor activity), GO:0005515 (protein binding), GO:0035591 (signaling adaptor activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0044237 (cellular metabolic process), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:0051716 (cellular response to stimulus), GO:0071840 (cellular component organization or biogenesis), GO:0044238 (primary metabolic process), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0033036 (macromolecule localization), GO:1901360 (organic cyclic compound metabolic process), GO:1903047 (mitotic cell cycle process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0044786 (cell cycle DNA replication), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0000278 (mitotic cell cycle), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0033260 (nuclear DNA replication), GO:1902969 (mitotic DNA replication), GO:0006259 (DNA metabolic process), GO:0008104 (protein localization), GO:0006996 (organelle organization), GO:0045005 (DNA-templated DNA replication maintenance of fidelity), GO:0006260 (DNA replication), GO:0000723 (telomere maintenance), GO:0006281 (DNA repair), GO:0051276 (chromosome organization), GO:0032200 (telomere organization), GO:0006261 (DNA-templated DNA replication), GO:0031297 (replication fork processing), GO:0006302 (double-strand break repair)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0032993 (protein-DNA complex), GO:0000785 (chromatin), GO:0005829 (cytosol), GO:0090734 (site of DNA damage), GO:0140513 (nuclear protein-containing complex), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0035861 (site of double-strand break), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005694 (chromosome), GO:0005634 (nucleus)