Protein: Mitochondrial-processing peptidase subunit alpha (Alpha-MPP) (Inactive zinc metalloprotease alpha) (P-55) (P20069)
Organism: Rattus norvegicus (Rat)
Function: Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins.
Subcellular location: Mitochondrion matrix {ECO:0000269|PubMed:2236012}. Mitochondrion inner membrane {ECO:0000250|UniProtKB:Q10713}.
Sequence: MATAVWAAARLLRGSAALCARPKFGSPAHRRFSSGATYPNIPLSSPLPGVPKPIFATVDGQEKFETKVTTLDNGLRVASQNKFGQFCTLGILINSGSRYEAKYLSGIAHFLEKLAFSSTARFDSKDEILLTLEKHGGICDCQTSRDTTMYAVSADSKGLDTVVGLLADVVLHPRLTDEEIEMTRMAVQFELEDLNMRPDPEPLLTEMIHEAAFRENTVGLHRFCPVENIGKIDREVLHSYLKNYYTPDRMVLAGVGVEHEHLVECARKYLLGVQPAWGAPGAVWMLTAQWHSTRGGSSRWRETCQMSALRPPRFQSSHIYGGARELLLLEEDFIPFAVLNMMMGGGGSFSAGGPGKGMFSRLYLNVLNRHHWMYNATSYHHSYEDTGLLCIHASADPRQVREMVEIITKEFILMGRTVDLVELERAKTQLMSMLMMNLESRPVIFEDVGRQVLATHSRKLPHELCTLIRNVKPEDIKRVASKMLRGKPAVAALGDLTDLPTYEHIQAALSSRDGRLPRTYRLFR
InterPro domains: - IPR007863: Peptidase M16, C-terminal (domain) [231-430]
- IPR050361: Mitochondrial Processing Peptidase/Ubiquinol-cytochrome c Reductase Complex (family) [60-500]
- IPR001431: Peptidase M16, zinc-binding site (binding_site) [96-119]
- IPR011765: Peptidase M16, N-terminal (domain) [76-225]
- IPR011249: Metalloenzyme, LuxS/M16 peptidase-like (homologous_superfamily) [63-505]
Interaction partners: - Mitochondrial-processing peptidase subunit beta
- Cytochrome c1, heme protein, mitochondrial
- Cytochrome b-c1 complex subunit Rieske, mitochondrial
- NADH dehydrogenase flavoprotein 1, mitochondrial
- NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
- Cytochrome b-c1 complex subunit 1, mitochondrial
- Cytochrome c oxidase subunit 5A, mitochondrial
- Cytochrome b-c1 complex subunit 8
- Cytochrome b-c1 complex subunit 7
- NADH dehydrogenase flavoprotein 2, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0008233 (peptidase activity), GO:0004175 (endopeptidase activity), GO:0008237 (metallopeptidase activity), GO:0004222 (metalloendopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0006807 (nitrogen compound metabolic process), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0033036 (macromolecule localization), GO:1901564 (organonitrogen compound metabolic process), GO:0046907 (intracellular transport), GO:0006810 (transport), GO:0019538 (protein metabolic process), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0043170 (macromolecule metabolic process), GO:0045184 (establishment of protein localization), GO:0006839 (mitochondrial transport), GO:0006886 (intracellular protein transport), GO:0071705 (nitrogen compound transport), GO:0006508 (proteolysis), GO:0015031 (protein transport), GO:0072594 (establishment of protein localization to organelle), GO:0051604 (protein maturation), GO:0071702 (organic substance transport), GO:0008104 (protein localization), GO:0006996 (organelle organization), GO:0010467 (gene expression), GO:0072655 (establishment of protein localization to mitochondrion), GO:0007005 (mitochondrion organization), GO:0033365 (protein localization to organelle), GO:0006605 (protein targeting), GO:0006626 (protein targeting to mitochondrion), GO:0016485 (protein processing), GO:0070585 (protein localization to mitochondrion), GO:0034982 (mitochondrial protein processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)