Protein: Murinoglobulin-1 (MuG1) (P28665)
Organism: Mus musculus (Mouse)
Function: A proteinase activates the inhibitor by specific proteolysis in the bait region, which, by an unknown mechanism leads to reaction at the cysteinyl-glutamyl internal thiol ester site and to a conformational change, whereby the proteinase is trapped and/or covalently bound to the inhibitor. While in the tetrameric proteinase inhibitors steric inhibition is sufficiently strong, monomeric forms need a covalent linkage between the activated glutamyl residue of the original thiol ester and a terminal amino group of a lysine or another nucleophilic group on the proteinase, for inhibition to be effective.
Subcellular location: Secreted.
Sequence: MWKSRRAQLCLFSVLLAFLHSASLLNGDSKYMVLVPSQLYTETPEKICLHLYQLNETVTVTASLVSQSGRKNLFDELVLDKDLFQCVSFIIPRLSSSDEEDFLYVDIKGPTHEFSKRKAVLVKNKESVVFVQTDKPVYKPGQSVKFRVVSMDKMLRPLNELLPLAYIEDPKKNRIMQWRDIKTENGLKQMSFSLAAEPIQGPYKIVVHKESGEKEEHSFTVMEFVLPRFNVDLKVPNAMSVNDEVLSVTACGKYTYGKPVPGHVKINVCRETETGCREVNSQLDNNGCSTQEVNITELQSKKRNYEVQLFHVNATVTEEGTGLEFSRSGTTKIERITNKLIFLKADSHFRHGIPFFVKVRLVDIKGDPIPNEKVFIKAQELSYTSATTTDQHGLAEFSIDTTCISGSSLHIKVNHKEEDSCSYFYCMEERHASAKHVAYAVYSLSKSYIYLDTETSSILPCNQIHTVQAHFILKGDLGVLKELIFYYLVMAQGSIIQTGNHTHQVEPGEAPVKGKFALEIPVEFSMVPMAKMLIYTILPDGEVIADSVNFEIEKCLRNKVDLRFSTSQSLPASQTRLQVTASPQSLCGLRAVDQSVLLLKPESELSPSWIYNLPGMQQNKFVPSSRLSEDQEDCILYSSWLAEKHTNLVPHGTEKDVYRYVEDMGLTAFTNLMIKLPIICFDYGMVPISAPRVEFDLAFTPEISWSLRTTLSKRPEEPPRKDPSSNDPLTETIRKYFPETWVWDIVTVNSTGLAEVEMTVPDTITEWKAGALCLSNDTGLGLSSVVPLQAFKPFFVEVSLPYSVVRGEAFMLKATVMNYLPTSMQMSVQLEASPDFTAVPVGDDQDSYCLSANGRHTSSWLVTPKSLGNVNFSVSAEAQQSSEPCGSEVATVPETGRKDTVVKVLIVEPEGIKQEHTFSSLFCASDAEISEKMSLVLPPTVVKDSARAHFSVMGDILSSAIRNTQNLLHMPYGCGEQNMVLFAPNIYVLKYLNETQQLTQKIKTKALGFLRAGYQRELNYKHKDGSYSAFGDQNGEREGNTWLTAFVLKSFAQARAFIFIDESHITHAFTWLSQKQKDNGCFRSSGSLFNNAMKGGVDDEMTLSAYITMALLESSLPATHPVVSKALSCLESSWKTIEQERNASFVYTKALMAYAFALAGNQNKRDEILKSLDEEAIKENNSIHWKRPQKSRKSEHHLYKPQASSAEVEMNAYVVLARLTAQPAPSPEDLTLSMSTIMWLTKQQNSNGGFSSTQDTVVALDALSKYGAVTFSRSQKTTLVTIQSTGSFSQKFQVENSNRLLLQQVALPDIPGDYTISVSGEGCVYAQTMLRYNMHLEKQLSAFAIWVQTVPLTCNNPKGHNSFQISLEISYTGSRPASNMVIADVKMLSGFIPLKPTVKKLERLEHVSRTEVSNNNVLIYLDQVTNQTLAFSFIIQQDIPVRNLQPAIVKVYDYYETDEMAFAEYSSPCSTDKQNV
InterPro domains: - IPR008930: Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid (homologous_superfamily) [960-1269]
- IPR050473: Alpha-2-macroglobulin/Complement system (family) [10-1473]
- IPR009048: Alpha-macroglobulin, receptor-binding (domain) [1377-1466]
- IPR041813: Alpha-2-macroglobulin, TED domain (domain) [960-1266]
- IPR036595: Alpha-macroglobulin, receptor-binding domain superfamily (homologous_superfamily) [1341-1476]
- IPR041555: Macroglobulin domain MG3 (domain) [223-301]
- IPR013783: Immunoglobulin-like fold (homologous_superfamily) [339-908]
- IPR001599: Alpha-2-macroglobulin (domain) [740-830]
- IPR011626: Alpha-macroglobulin-like, TED domain (domain) [951-1266]
- IPR010916: TonB box, conserved site (conserved_site) [1-63]
- IPR019742: Alpha-2-macroglobulin, conserved site (conserved_site) [971-979]
- IPR011625: Alpha-2-macroglobulin, bait region domain (domain) [449-599]
- IPR014756: Immunoglobulin E-set (homologous_superfamily) [788-908]
- IPR002890: Macroglobulin domain (domain) [129-221]
- IPR047565: Alpha-macroglobulin-like, thiol-ester bond-forming region (conserved_site) [963-992]
- IPR040839: Macroglobulin domain MG4 (domain) [341-434]
Interaction partners: - UDP-glucuronosyltransferase
- Carboxylesterase 3B
- Cytochrome P450 2C50
- Serine protease inhibitor A3K
- Major urinary protein 3
- Cytochrome P450 2A12
- Carboxylesterase 3A
- Estradiol 17 beta-dehydrogenase 5
- Murinoglobulin-2
- Apolipoprotein A-V
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0030234 (enzyme regulator activity), GO:0140678 (molecular function inhibitor activity), GO:0061134 (peptidase regulator activity), GO:0004857 (enzyme inhibitor activity), GO:0061135 (endopeptidase regulator activity), GO:0030414 (peptidase inhibitor activity), GO:0004866 (endopeptidase inhibitor activity)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0022414 (reproductive process), GO:0007566 (embryo implantation), GO:0048856 (anatomical structure development), GO:0044706 (multi-multicellular organism process), GO:0007275 (multicellular organism development), GO:0044703 (multi-organism reproductive process), GO:0007565 (female pregnancy)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0030312 (external encapsulating structure), GO:0005576 (extracellular region), GO:0031012 (extracellular matrix), GO:0062023 (collagen-containing extracellular matrix)