Protein: DASH complex subunit ASK1 (Q5AMH1)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Component of the DASH complex that connects microtubules with kinetochores and couples microtubule depolymerisation to chromosome movement; it is involved in retrieving kinetochores to the spindle poles before their re-orientation on the spindle in early mitosis and allows microtubule depolymerization to pull chromosomes apart and resist detachment during anaphase (By similarity). Kinetochores, consisting of a centromere-associated inner segment and a microtubule- contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and microtubules (By similarity). Kinetochores also serve as an input point for the spindle assembly checkpoint, which delays anaphase until all chromosomes have bioriented on the mitotic spindle
Subcellular location: Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Nucleus
Sequence: MKRYSIAPTSSRRKSAIDATLPESNLPARQILEQLDQDTTFVLQEIDKNISRANTVINDKIKPMIQEYAFQSWKVWENTGFWKHFFEQSANVELDSYVEPINEVNPQNNFLLLNEIEKNKQKGRYEDSRGSNKVNQANNDENNDIDNDEKEEPEIAFKKPNLRRLVADGNTPTWSTEHTKPSMKSIQLSPPKRMQTTNFTGGLARENTKLTDSITLEPPALSAETRAVTSSPHSRHYQNQHQRLQSRDSPIKIHTIRQSLDTYHRVSISPKKLKTPIRSTRTSRIESILNSSPILPEPPVLQSEIGSETPKYMSSSSPMKKQQQQQRESLNFQSDNDQQQGLQRFPNTPKYSRLSGSSVNSLLSNKSNLSDDEDDHINPPPQLDSDNHGVVIDNSDTNNNNGDSSDDLAALPVPELNTIDFKDKNSNNNNKRSIQSTPRSSEKKRRSINTKNTNNNNTSAAATAVVVDDDDDRENVFLDHSYKKTPDNQQSNNSTRFFSTGGSNNDNHTETQDNSKSFSQIYDEAISKIRGKKTTSQSNDQNGDEDGDVVMSLDMTVTKDLTGEPTKDLSKDLTENSTTDLGPFRERWKKLTRK
InterPro domains: - IPR013964: DASH complex subunit Ask1 (family) [1-569]
Interaction partners: - Tubulin gamma chain
- Spindle pole body component
- DASH complex subunit DAD3
- DASH complex subunit DAD2
- DASH complex subunit DAM1
- DASH complex subunit SPC34
- DASH complex subunit SPC19
- DASH complex subunit DAD1
- DASH complex subunit DUO1
- Actin-related protein 5
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003677 (DNA binding), GO:0003690 (double-stranded DNA binding), GO:0043565 (sequence-specific DNA binding), GO:1990837 (sequence-specific double-stranded DNA binding), GO:0019237 (centromeric DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:0007059 (chromosome segregation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098687 (chromosomal region), GO:0099080 (supramolecular complex), GO:0000940 (outer kinetochore), GO:0140513 (nuclear protein-containing complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000775 (chromosome, centromeric region), GO:0000776 (kinetochore), GO:0043228 (non-membrane-bounded organelle), GO:0000779 (condensed chromosome, centromeric region), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome), GO:0005634 (nucleus), GO:0000793 (condensed chromosome)