Protein: Alanine--tRNA ligase (EC 6.1.1.7) (Alanyl-tRNA synthetase) (AlaRS) (O13914)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Subcellular location: Mitochondrion {ECO:0000255|HAMAP-Rule:MF_03133}. Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03133}.
Sequence: MTAESEVVNWPANEIRRTFLKYFEDHGHTIVPSSSVIPYDDPTLLFANAGMNQFKPIFLGTVDPSSDFAKLKRACDSQKCIRAGGKHNDLEDVGKDNYHHTMFEMLGNWSFGDYFKKEAIAMAWDLLTNVYGLKKDQLYVTYFGGHEESGLEPDLEARQLWLDIGIDESRVIPGSLKDNFWEMGDQGPCGPCSEIHYDRIGNRTVPELVNMDDPNVLEIWNIVFIQFNREKDGSLRPLPNRHVDTGMGFERLVSVIQNKTSNYDTDVFSPIFAKIQELTNARPYTGKMGDEDVDGIDTAYRVVADHVRTLTFAISDGGVPNNEGRGYVLRRILRRGARYVRKKFGVPIGNFFSRLSLTVVEQMGDFFPELKRKVDDVRELLDEEEESFSRTLDRGEKMFEQYAAAAKKTPSKTLQGNDVWRLYETYGFPVDLTHLMAEEAGIKIDEPGFEAAQARSKEISKQASKGGSSGDDLLVLDVHALGALSKMDDIPETDDVFKHNSVSLKSVIKGIYHKGGFQKSTEGFNSGEQLGLLLDRTNFYAEQGGQEYDTGHIVIDGVADFRVTNVQVYAGYVLHTGFLEYGNLTVNDSVVCEYDEIRRWHLMNNHTVTHILNLALRNTLGDGIDQRGSLVSQEKLRFDFSYKSSIPIDKLLLVENYCNNVIQDNLSVYSKEVALSKAKEINGLRAVFGEVYPDPVRVVCIGVDIDTLLQEPKKPDWTKYSIEFCGGTHCDKSGEIKDFVILEENGIAKGIRRIVAVTSTEANRVSRLANEFDARIAKLEKMPFSPAKEAELKKISVDLSKLVVAAVRKHAMKERIAKITKQVQEHVKAINAAEQKEVVNVVTEYFKENPDMSFVVAKVPISANPKALSFALTYAKKNLKDKSIYLLASDDTKVAHACLVSPEAMKKLTPQEWSQKVCHSIGGRSGGKGDTCQGVGDKPLSIDVAVEEAIEFFQGKLTI
InterPro domains: - IPR012947: Threonyl/alanyl tRNA synthetase, SAD (domain) [696-755]
- IPR050058: Alanine--tRNA ligase (family) [6-953]
- IPR045864: Class II Aminoacyl-tRNA synthetase/Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL) (homologous_superfamily) [11-263]
- IPR002318: Alanine-tRNA ligase, class IIc (family) [14-935]
- IPR003156: DHHA1 domain (domain) [852-954]
- IPR023033: Alanine-tRNA ligase, eukaryota/bacteria (family) [1-957]
- IPR018164: Alanyl-tRNA synthetase, class IIc, N-terminal (domain) [14-596]
- IPR009000: Translation protein, beta-barrel domain superfamily (homologous_superfamily) [526-592]
- IPR018162: Alanine-tRNA ligase, class IIc, anti-codon-binding domain superfamily (homologous_superfamily) [264-475]
- IPR018165: Alanyl-tRNA synthetase, class IIc, core domain (domain) [10-768]
- IPR018163: Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily (homologous_superfamily) [598-761]
Interaction partners: - Origin recognition complex subunit 4
- Putative glycine--tRNA ligase
- Anaphase-promoting complex subunit cut9
- Anaphase-promoting complex subunit 5
- Anaphase-promoting complex subunit 10
- Anaphase-promoting complex subunit 1
- Anaphase-promoting complex subunit 4
- Anaphase-promoting complex subunit 11
- Anaphase-promoting complex subunit 2
- Aspartate--tRNA ligase, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0140098 (catalytic activity, acting on RNA), GO:0016875 (ligase activity, forming carbon-oxygen bonds), GO:0004812 (aminoacyl-tRNA ligase activity), GO:0140101 (catalytic activity, acting on a tRNA)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006082 (organic acid metabolic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006412 (translation), GO:0009059 (macromolecule biosynthetic process), GO:0006518 (peptide metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0043038 (amino acid activation), GO:0010467 (gene expression), GO:0019752 (carboxylic acid metabolic process), GO:0016070 (RNA metabolic process), GO:0043043 (peptide biosynthetic process), GO:0006418 (tRNA aminoacylation for protein translation), GO:0043039 (tRNA aminoacylation), GO:0034660 (ncRNA metabolic process), GO:0006399 (tRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)