Protein: Small ribosomal subunit protein uS3 (40S ribosomal protein S3) (RP13) (YS3) (P05750)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:22096102}.
Sequence: MVALISKKRKLVADGVFYAELNEFFTRELAEEGYSGVEVRVTPTKTEVIIRATRTQDVLGENGRRINELTLLVQKRFKYAPGTIVLYAERVQDRGLSAVAQAESMKFKLLNGLAIRRAAYGVVRYVMESGAKGCEVVVSGKLRAARAKAMKFADGFLIHSGQPVNDFIDTATRHVLMRQGVLGIKVKIMRDPAKSRTGPKALPDAVTIIEPKEEEPILAPSVKDYRPAEETEAQAEPVEA
InterPro domains: - IPR057258: Small ribosomal subunit protein uS3 (family) [7-204]
- IPR018280: Small ribosomal subunit protein uS3, conserved site (conserved_site) [147-183]
- IPR015946: K homology domain-like, alpha/beta (homologous_superfamily) [1-91]
- IPR036419: Ribosomal protein S3, C-terminal domain superfamily (homologous_superfamily) [93-240]
- IPR001351: Small ribosomal subunit protein uS3, C-terminal (domain) [107-188]
- IPR004044: K Homology domain, type 2 (domain) [20-93]
- IPR009019: K homology domain superfamily, prokaryotic type (homologous_superfamily) [13-99]
- IPR005703: Small ribosomal subunit protein uS3, eukaryotic/archaeal (family) [8-210]
Interaction partners: - Small ribosomal subunit protein eS10A
- Large ribosomal subunit protein eL24B
- Small ribosomal subunit protein eS27A
- Small ribosomal subunit protein uS14B
- Large ribosomal subunit protein eL19A
- Small ribosomal subunit protein eS7B
- Small ribosomal subunit protein uS11B
- Small ribosomal subunit protein eS4A
- Small ribosomal subunit protein eS1A
- Large ribosomal subunit protein uL6A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005198 (structural molecule activity), GO:0005488 (binding), GO:0003735 (structural constituent of ribosome), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0019843 (rRNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044085 (cellular component biogenesis), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006412 (translation), GO:0009059 (macromolecule biosynthetic process), GO:0006518 (peptide metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0043933 (protein-containing complex organization), GO:0006996 (organelle organization), GO:0010467 (gene expression), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0042254 (ribosome biogenesis), GO:0042274 (ribosomal small subunit biogenesis), GO:0016070 (RNA metabolic process), GO:0043043 (peptide biosynthetic process), GO:0022618 (ribonucleoprotein complex assembly), GO:0065003 (protein-containing complex assembly), GO:0090305 (nucleic acid phosphodiester bond hydrolysis), GO:0002181 (cytoplasmic translation), GO:0006396 (RNA processing), GO:0071826 (ribonucleoprotein complex subunit organization), GO:0000028 (ribosomal small subunit assembly), GO:0090501 (RNA phosphodiester bond hydrolysis), GO:0034470 (ncRNA processing), GO:0042255 (ribosome assembly), GO:0140694 (non-membrane-bounded organelle assembly), GO:0030490 (maturation of SSU-rRNA), GO:0006364 (rRNA processing), GO:0034660 (ncRNA metabolic process), GO:0000469 (cleavage involved in rRNA processing), GO:0090502 (RNA phosphodiester bond hydrolysis, endonucleolytic), GO:0016072 (rRNA metabolic process), GO:0000462 (maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)), GO:0000478 (endonucleolytic cleavage involved in rRNA processing), GO:0000447 (endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)), GO:0000466 (maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)), GO:0000479 (endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA))
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:1990904 (ribonucleoprotein complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0044391 (ribosomal subunit), GO:0043229 (intracellular organelle), GO:0022626 (cytosolic ribosome), GO:0043228 (non-membrane-bounded organelle), GO:0022627 (cytosolic small ribosomal subunit), GO:0015935 (small ribosomal subunit), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005840 (ribosome)