Protein: Alpha-dioxygenase 1 (Alpha DOX1) (EC 1.13.11.92) (Fatty acid dioxygenase AlphaDOX1) (Pathogen-induced oxygenase) (Plant alpha dioxygenase 1) (Q9SGH6)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Alpha-dioxygenase that catalyzes the primary oxygenation step of a variety of 14-20 carbon fatty acids, containing up to three unsaturated bonds, into their corresponding 2R-hydroperoxides. Involved in the production of oxylipins that function in cell signaling, wound healing, and protection from infection. Mediates protection against oxidative stress and cell death, probably by generating some lipid-derived molecules. Promotes local and systemic plant defense in a salicylic acid (SA)-dependent manner, including the establishment of systemic acquired resistance (SAR) in response to incompatible interaction. Involved in a negative regulation of abscisic acid (ABA)-mediated signaling pathway.
Subcellular location: Lipid droplet {ECO:0000269|PubMed:24214535}. Note=Localizes on the surface of leaf oil bodies. {ECO:0000269|PubMed:24214535}.
Sequence: MKVITSLISSILLKFIHKDFHEIYARMSLLDRFLLLIVHGVDKMVPWHKLPVFLGLTYLEVRRHLHQQYNLLNVGQTPTGIRFDPANYPYRTADGKFNDPFNEGVGSQNSFFGRNCPPVDQKSKLRRPDPMVVATKLLGRKKFIDTGKQFNMIAASWIQFMIHDWIDHLEDTHQIELVAPKEVASKCPLSSFRFLKTKEVPTGFFEIKTGSQNIRTPWWDSSVIYGSNSKTLDRVRTYKDGKLKISEETGLLLHDEDGLAISGDIRNSWAGVSALQALFIKEHNAVCDALKDEDDDLEDEDLYRYARLVTSAVVAKIHTIDWTVQLLKTDTLLAGMRANWYGLLGKKFKDSFGHAGSSILGGVVGMKKPQNHGVPYSLTEDFTSVYRMHSLLPDQLHILDIDDVPGTNKSLPLIQEISMRDLIGRKGEETMSHIGFTKLMVSMGHQASGALELMNYPMWLRDIVPHDPNGQARPDHVDLAALEIYRDRERSVPRYNEFRRSMFMIPITKWEDLTEDEEAIEVLDDVYDGDVEELDLLVGLMAEKKIKGFAISETAFYIFLIMATRRLEADRFFTSDFNETIYTKKGLEWVNTTESLKDVIDRHYPDMTDKWMNSESAFSVWDSPPLTKNPIPLYLRIPS
InterPro domains: - IPR010255: Haem peroxidase superfamily (homologous_superfamily) [86-636]
- IPR050783: Oxylipin biosynthesis and metabolism (family) [12-636]
- IPR019791: Haem peroxidase, animal-type (family) [81-639]
- IPR034815: Alpha-dioxygenase (family) [91-622]
- IPR037120: Haem peroxidase domain superfamily, animal type (homologous_superfamily) [27-639]
Interaction partners: - Lipoxygenase 2, chloroplastic
- Lipoxygenase 4, chloroplastic
- Lipoxygenase 3, chloroplastic
- Lipoxygenase 6, chloroplastic
- Triacylglycerol lipase SDP1
- Alpha-dioxygenase 2
- Phospholipase A
- Lecithin-cholesterol acyltransferase-like 4
- Phospholipase A2-alpha
- Phospholipase A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0051213 (dioxygenase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0090693 (plant organ senescence), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0099402 (plant organ development), GO:0006629 (lipid metabolic process), GO:0010150 (leaf senescence), GO:0006082 (organic acid metabolic process), GO:0048731 (system development), GO:0044255 (cellular lipid metabolic process), GO:0048367 (shoot system development), GO:0006631 (fatty acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:0048827 (phyllome development), GO:0019752 (carboxylic acid metabolic process), GO:0001676 (long-chain fatty acid metabolic process), GO:0048366 (leaf development), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle)