Protein: Elongator complex protein 2 (Q7K4B3)
Organism: Drosophila melanogaster (Fruit fly)
Function: Component of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. Binding by the elongator complex stabilizes microtubules and promotes their growth. This induces central spindle asymmetry, promoting polarized signaling endosome trafficking during asymmetric cell division and cell fate assignation of sensory organ precursor cells. Involved in the regulation of the STAT pathway (Ref.4)
Subcellular location: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=During asymmetric cell division of sensory organ precursor cells the elongator complex preferentially binds and stabilizes microtubules on the anterior side (pIIb daughter cell) of the spindle
Sequence: MQVENLHTSVACNRCTECADWGPNGWIAYGACNAIAIMDPKFQGNSAKVLFTLVEHTKRVNTVRWLDCDKLLSGGDDAIAILWELDETGTTKSFTLKGHTSGVNTVDGIRQQDGSWLLATAAADTTIKLWTFQDNNYVCFQTISLSDGFCFCLRLQLLPKSNQVLLAFSGDDETVSLWSEQVETAGEGDSLGRQFQRKHKLTGHEDWVRGLDFVVDGEDLLLASGSQDNFIRLWRIAPRSKEQMQENRVDLHQLSHNDDEIKVEEKILQLGKEAWYAVSLESVLYGHEGWIYGVHWHKTPDQELRLLSASIDKTVIIWAPTEEGIWLEEVRVGEVGGNSVGFYGGKFSGDGHSIMAHSYQGGFHIWSQDPDRPQLWTPGVIVGGHYGEVRDLAWEHSGAYLMTASADQTTRLHAPWLQDGANPTWHELARPQIHGYDMQALALLSRYKFASGAEEKIVRTFQAPANFIENFRHISGIENDDAGDVLLDSLPKGASVPSLGLSNKAVYKVDSEVESTSKTSKDEYPDNYFVPIALETPPQEETLMQNTLWPELQKLYGHGYEIFALAATADGSLLASTCKASNAEHAQIILWNPSNWKQIQKLSGHQLTVTQLSFSPDSRYLLSVSRDRRWCLYERQDSSVSYQLVASTDKSNGVHTRIIWSCDWSHDGQFFVTSSRDGKVVVWKKEEDCKESSLNGWQANGVLELKNESITAVAFSNSYLSGTDDTYILALGTETGLIKIYQFVRGAWKLLSDLNKSQAHHLTVRRLQFRPGKQLQLASCGEDHLVRIYDIKLT
InterPro domains: - IPR037289: Elongator complex protein 2 (family) [1-793]
- IPR036322: WD40-repeat-containing domain superfamily (homologous_superfamily) [6-792]
- IPR015943: WD40/YVTN repeat-like-containing domain superfamily (homologous_superfamily) [4-794]
- IPR001680: WD40 repeat (repeat) [46-790]
- IPR019775: WD40 repeat, conserved site (conserved_site) [71-85]
Interaction partners: - Elongator complex protein 1
- Elongator complex protein 3
- Elongator complex protein 5
- Elongator complex protein 4
- Tetraspanin 68C, isoform A
- Protein KTI12 homolog
- Ribosome biogenesis regulatory protein
- DNA-directed RNA polymerase I subunit RPA1
- DNA-directed RNA polymerase III subunit RPC2
- LD47749p
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0019899 (enzyme binding), GO:0019900 (kinase binding), GO:0019901 (protein kinase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0031323 (regulation of cellular metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0010467 (gene expression), GO:0010468 (regulation of gene expression), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0016070 (RNA metabolic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006355 (regulation of DNA-templated transcription), GO:0006357 (regulation of transcription by RNA polymerase II), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0006351 (DNA-templated transcription), GO:0006354 (DNA-templated transcription elongation), GO:0006368 (transcription elongation by RNA polymerase II), GO:0006366 (transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0140535 (intracellular protein-containing complex)