Protein: Mediator of RNA polymerase II transcription subunit 12 (Mediator complex subunit 12) (Suppressor of RNA polymerase B 8) (P25648)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5.
Subcellular location: Nucleus {ECO:0000305}.
Sequence: MNNGSGRYLLTPPDDLHPYVPSSKPQEQVYPDFKPWEHTAAEDQILANFVAKGFYHTPMVNFESISARSSVHESLVTQSNILSQQFDKIIKIREDHINKIPSNSTTTLHGPGFQLPNRITLTDHRKETWLHELSSSHTSLVKIGKFIPHGLKRRQVIEQCYLKFIPLKRAIWLIKCCYFIEWKSNHKKKRSNAAGADDAISMHLLKDWTDTFVYILEKLIFDMTNHYNDSQQLRTWKRQISYFLKLLGNCYSLRLINKEIFHHWLVEFINKMENFEFLPLSLHILMIFWNDICQIDTNAPVAATITSSQKEPFFLVTKITDMLLHKYYIVSSSKSMINDENYIINDIKKNNKIKLNILKILSSLILKIFQEQSLEVFIFPTSNWEIYKPLLFEIVSNADTNQNSDMKKKLELISYRNESLKNNSSIRNVIMSASNANDFQLTIVTCKQFPKLSCIQLNCIDTQFTKLLDDNPTEFDWPTYVDQNPLTMHKIIQLILWSIHPSRQFDHYESNQLVAKLLLLRINSTDEDLHEFQIEDAIWSLVFQLAKNFSAQKRVVSYMMPSLYRLLNILITYGIIKVPTYIRKLISSGLLYLQDSNDKFVHVQLLINLKISPLMKSQYNMVLRNVMEYDVKFYEIFNFDQLVEITEQIKMRILSNDITNLQLSKTPLSIKIMVAEWYLSHLCSGILSSVNRTVLLKIFKIFCIDLEVFHHFFKWIEFIVYHQLLSDIESLEALMDILLCYQKLFSQFINDHILFTKTFIFIYKKVLKEKDVPAYNVTSFMPFWKFFMKNFPFVLKVDNDLRIELQSVYNDEKLKTEKLKNDKSEVLKVYSMINNSNQAVGQTWNFPEVFQVNIRFLLHNSEIIDTNTSKQFQKARNNVMLLIATNLKEYNKFMSIFLKRKDFTNKNLIQLISLKLLTFEVTQNVLGLEYIIRLLPINLENNDGSYGLFLKYHKEQFIKSNFEKILLTCYELEKKYHGNECEINYYEILLKILITYGSSPKLLATSTKIIMLLLNDSVENSSNILEDILYYSTCPSETDLNDIPLGSGQPDNDTVVTNDDKSDDDDHTVDEIDHVEYYVMMDFANLWVFQAFTCFCIKKIMENNEPAMAMEDLKNFIFQIIEITNSNDLCSQIFDQLKDMQTIEMITQIVEKDFCTSCLQNNNQKIDDNYIVVVIEIITSLSMRFQRETSGMIVISMENYHLLIKIIRQLSELNEGNLSKREIQIDAVLKIFSFHQDSIFQRIIADLSADKPTSPFIDSICKLFDKISFNLRLKLFLYEILSSLKSFAIYSSTIDAPAFHTSGKVELPKKLLNLPPFQVSSFVKETKLHSGDYGEEEDADQEESFSLNLGIGIVEIAHENEQKWLIYDKKDHKYVCTFSMEPYHFISNYNTKYTDDMATGSNDTTAFNDSCVNLSLFDARFERKNPH
InterPro domains: - IPR019035: Mediator complex, subunit Med12 (domain) [112-175]
Interaction partners: - Meiotic mRNA stability protein kinase SSN3
- RNA polymerase II holoenzyme cyclin-like subunit
- Mediator of RNA polymerase II transcription subunit 13
- Mediator of RNA polymerase II transcription subunit 16
- ADA histone acetyltransferase complex component 2
- Mediator of RNA polymerase II transcription subunit 5
- Mediator of RNA polymerase II transcription subunit 2
- Mediator of RNA polymerase II transcription subunit 21
- Mediator of RNA polymerase II transcription subunit 19
- Mediator of RNA polymerase II transcription subunit 7
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0009892 (negative regulation of metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0044248 (cellular catabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0034655 (nucleobase-containing compound catabolic process), GO:0009057 (macromolecule catabolic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0090304 (nucleic acid metabolic process), GO:0044265 (cellular macromolecule catabolic process), GO:0019439 (aromatic compound catabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0046700 (heterocycle catabolic process), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression), GO:0016070 (RNA metabolic process), GO:0006402 (mRNA catabolic process), GO:0006401 (RNA catabolic process), GO:0016071 (mRNA metabolic process), GO:0000956 (nuclear-transcribed mRNA catabolic process), GO:0000184 (nuclear-transcribed mRNA catabolic process, nonsense-mediated decay)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140513 (nuclear protein-containing complex), GO:0043229 (intracellular organelle), GO:0016592 (mediator complex), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)