Protein: Nitric oxide synthase 1 (EC 1.14.13.39) (Constitutive NOS) (NC-NOS) (NOS type I) (Neuronal NOS) (N-NOS) (nNOS) (Nitric oxide synthase, brain) (bNOS) (Peptidyl-cysteine S-nitrosylase NOS1) (Q9Z0J4)
Organism: Mus musculus (Mouse)
Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Isoform NNOS Mu may be an effector enzyme for the dystrophin complex.
Subcellular location: Cell membrane, sarcolemma {ECO:0000269|PubMed:7545544}; Peripheral membrane protein {ECO:0000255}. Cell projection, dendritic spine {ECO:0000250|UniProtKB:P29476}. Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex (PubMed:7545544). In neurons, enriched in dendritic spines (By similarity). {ECO:0000250|UniProtKB:P29476, ECO:0000269|PubMed:7545544}.
Sequence: MEEHTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGTPTKAVDLSRQPSASKDQPLAVDRVPGPSNGPQHAQGRGQGAGSVSQANGVAIDPTMKNTKANLQDSGEQDELLKEIEPVLSILTGGGKAVNRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLGGENDRVFNDLWGKGNVPVVLNNPYSENEQSPASGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVEFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS
InterPro domains: - IPR036034: PDZ superfamily (homologous_superfamily) [4-126]
- IPR001433: Oxidoreductase FAD/NAD(P)-binding (domain) [1246-1358]
- IPR017927: FAD-binding domain, ferredoxin reductase-type (domain) [990-1237]
- IPR044943: Nitric oxide synthase, domain 1 superfamily (homologous_superfamily) [314-679]
- IPR012144: Nitric-oxide synthase, eukaryote (family) [80-1422]
- IPR023173: NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamily (homologous_superfamily) [984-1171]
- IPR001094: Flavodoxin-like (domain) [756-919]
- IPR036119: Nitric oxide synthase, N-terminal domain superfamily (homologous_superfamily) [300-716]
- IPR003097: Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-binding (domain) [986-1214]
- IPR001478: PDZ domain (domain) [17-100]
- IPR050607: Nitric Oxide Synthase (NOS) (family) [274-1424]
- IPR001709: Flavoprotein pyridine nucleotide cytochrome reductase (domain) [1027-1353]
- IPR029039: Flavoprotein-like superfamily (homologous_superfamily) [742-950]
- IPR039261: Ferredoxin-NADP reductase (FNR), nucleotide-binding domain (homologous_superfamily) [1233-1397]
- IPR017938: Riboflavin synthase-like beta-barrel (homologous_superfamily) [964-1231]
- IPR044940: Nitric oxide synthase, domain 2 superfamily (homologous_superfamily) [431-542]
- IPR044944: Nitric oxide synthase, domain 3 superfamily (homologous_superfamily) [543-653]
- IPR004030: Nitric oxide synthase, N-terminal (domain) [351-712]
- IPR008254: Flavodoxin/nitric oxide synthase (domain) [755-935]
Interaction partners: - Calmodulin-like protein 3
- Calmodulin-like protein 4
- Calmodulin-1
- Calmodulin 5
- Calmodulin-4
- Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein
- Disks large homolog 4
- Glutamate receptor ionotropic, NMDA 1
- Glutamate receptor ionotropic, NMDA 2B
- Alpha-1-syntrophin
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0043167 (ion binding), GO:0005515 (protein binding), GO:0016491 (oxidoreductase activity), GO:0004497 (monooxygenase activity), GO:0043177 (organic acid binding), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0043168 (anion binding), GO:0044325 (transmembrane transporter binding), GO:0043169 (cation binding), GO:0031406 (carboxylic acid binding), GO:0016709 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen), GO:0016597 (amino acid binding)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0008152 (metabolic process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0022414 (reproductive process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0051641 (cellular localization), GO:0048856 (anatomical structure development), GO:0023057 (negative regulation of signaling), GO:0009653 (anatomical structure morphogenesis), GO:0042221 (response to chemical), GO:0006807 (nitrogen compound metabolic process), GO:0051241 (negative regulation of multicellular organismal process), GO:0051239 (regulation of multicellular organismal process), GO:0032504 (multicellular organism reproduction), GO:0003006 (developmental process involved in reproduction), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0009892 (negative regulation of metabolic process), GO:0007267 (cell-cell signaling), GO:0065008 (regulation of biological quality), GO:0050793 (regulation of developmental process), GO:0044706 (multi-multicellular organism process), GO:0009607 (response to biotic stimulus), GO:0050794 (regulation of cellular process), GO:0044703 (multi-organism reproductive process), GO:0048583 (regulation of response to stimulus), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0048523 (negative regulation of cellular process), GO:0051707 (response to other organism), GO:0007165 (signal transduction), GO:0007275 (multicellular organism development), GO:0009058 (biosynthetic process), GO:0051051 (negative regulation of transport), GO:0019222 (regulation of metabolic process), GO:0032879 (regulation of localization), GO:0051234 (establishment of localization), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0003008 (system process), GO:0033036 (macromolecule localization), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006950 (response to stress), GO:0140352 (export from cell), GO:0048609 (multicellular organismal reproductive process), GO:0048585 (negative regulation of response to stimulus), GO:0007154 (cell communication), GO:0044238 (primary metabolic process), GO:0007610 (behavior), GO:0090287 (regulation of cellular response to growth factor stimulus), GO:0009968 (negative regulation of signal transduction), GO:0043207 (response to external biotic stimulus), GO:0060548 (negative regulation of cell death), GO:1903828 (negative regulation of protein localization), GO:0044248 (cellular catabolic process), GO:0048731 (system development), GO:0044057 (regulation of system process), GO:0048513 (animal organ development), GO:0009966 (regulation of signal transduction), GO:0060255 (regulation of macromolecule metabolic process), GO:0008217 (regulation of blood pressure), GO:0048608 (reproductive structure development), GO:0045137 (development of primary sexual characteristics), GO:0003013 (circulatory system process), GO:0045184 (establishment of protein localization), GO:0051048 (negative regulation of secretion), GO:0034763 (negative regulation of transmembrane transport), GO:0010876 (lipid localization), GO:0010035 (response to inorganic substance), GO:0009887 (animal organ morphogenesis), GO:1904018 (positive regulation of vasculature development), GO:0002027 (regulation of heart rate), GO:1903523 (negative regulation of blood circulation), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0006082 (organic acid metabolic process), GO:0032940 (secretion by cell), GO:0009790 (embryo development), GO:1901575 (organic substance catabolic process), GO:0071496 (cellular response to external stimulus), GO:0090066 (regulation of anatomical structure size), GO:1903531 (negative regulation of secretion by cell), GO:0035239 (tube morphogenesis), GO:0010648 (negative regulation of cell communication), GO:1903530 (regulation of secretion by cell), GO:0051049 (regulation of transport), GO:1901564 (organonitrogen compound metabolic process), GO:0044282 (small molecule catabolic process), GO:0035556 (intracellular signal transduction), GO:0060341 (regulation of cellular localization), GO:0051224 (negative regulation of protein transport), GO:0019538 (protein metabolic process), GO:0051224 (negative regulation of protein transport), GO:1901700 (response to oxygen-containing compound), GO:0006520 (amino acid metabolic process), GO:0010817 (regulation of hormone levels), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0046883 (regulation of hormone secretion), GO:0001704 (formation of primary germ layer), GO:0048598 (embryonic morphogenesis), GO:0007565 (female pregnancy), GO:0042127 (regulation of cell population proliferation), GO:0010941 (regulation of cell death), GO:0009888 (tissue development), GO:0010646 (regulation of cell communication), GO:0070887 (cellular response to chemical stimulus), GO:0009617 (response to bacterium), GO:0006810 (transport), GO:0009889 (regulation of biosynthetic process), GO:0070727 (cellular macromolecule localization), GO:0003206 (cardiac chamber morphogenesis), GO:0070727 (cellular macromolecule localization), GO:0031323 (regulation of cellular metabolic process), GO:0001541 (ovarian follicle development), GO:0050877 (nervous system process), GO:0080090 (regulation of primary metabolic process), GO:0006790 (sulfur compound metabolic process), GO:0023061 (signal release), GO:0035592 (establishment of protein localization to extracellular region), GO:0016054 (organic acid catabolic process), GO:0061045 (negative regulation of wound healing), GO:0008015 (blood circulation), GO:0051246 (regulation of protein metabolic process), GO:0043069 (negative regulation of programmed cell death), GO:0043436 (oxoacid metabolic process), GO:0043067 (regulation of programmed cell death), GO:0070201 (regulation of establishment of protein localization), GO:0002237 (response to molecule of bacterial origin), GO:0031663 (lipopolysaccharide-mediated signaling pathway), GO:0071702 (organic substance transport), GO:0090257 (regulation of muscle system process), GO:0048662 (negative regulation of smooth muscle cell proliferation), GO:0050678 (regulation of epithelial cell proliferation), GO:0090278 (negative regulation of peptide hormone secretion), GO:1903522 (regulation of blood circulation), GO:0010468 (regulation of gene expression), GO:0051235 (maintenance of location), GO:0016054 (organic acid catabolic process), GO:0051223 (regulation of protein transport), GO:0090276 (regulation of peptide hormone secretion), GO:0043412 (macromolecule modification), GO:0090276 (regulation of peptide hormone secretion), GO:1901565 (organonitrogen compound catabolic process), GO:0050708 (regulation of protein secretion), GO:1904950 (negative regulation of establishment of protein localization), GO:1901701 (cellular response to oxygen-containing compound), GO:0003018 (vascular process in circulatory system), GO:0061458 (reproductive system development), GO:0010565 (regulation of cellular ketone metabolic process), GO:0032496 (response to lipopolysaccharide), GO:1901565 (organonitrogen compound catabolic process), GO:0009306 (protein secretion), GO:0071310 (cellular response to organic substance), GO:0009914 (hormone transport), GO:0046903 (secretion), GO:0032880 (regulation of protein localization), GO:1901605 (alpha-amino acid metabolic process), GO:0035150 (regulation of tube size), GO:0036211 (protein modification process), GO:0016054 (organic acid catabolic process), GO:0090087 (regulation of peptide transport), GO:0009306 (protein secretion), GO:0033993 (response to lipid), GO:0042742 (defense response to bacterium), GO:0070201 (regulation of establishment of protein localization), GO:0009063 (amino acid catabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0051046 (regulation of secretion), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0008104 (protein localization), GO:0060429 (epithelium development), GO:1901605 (alpha-amino acid metabolic process), GO:0009792 (embryo development ending in birth or egg hatching), GO:0046676 (negative regulation of insulin secretion), GO:0042981 (regulation of apoptotic process), GO:0008104 (protein localization), GO:1903844 (regulation of cellular response to transforming growth factor beta stimulus), GO:1901605 (alpha-amino acid metabolic process), GO:0046676 (negative regulation of insulin secretion), GO:0042981 (regulation of apoptotic process), GO:0001837 (epithelial to mesenchymal transition), GO:0006812 (monoatomic cation transport), GO:0061178 (regulation of insulin secretion involved in cellular response to glucose stimulus), GO:0050796 (regulation of insulin secretion), GO:0031960 (response to corticosteroid), GO:0046395 (carboxylic acid catabolic process), GO:0043523 (regulation of neuron apoptotic process), GO:0018193 (peptidyl-amino acid modification), GO:0019752 (carboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0030054 (cell junction), GO:0098794 (postsynapse), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0045202 (synapse), GO:0005886 (plasma membrane), GO:0099572 (postsynaptic specialization), GO:0098984 (neuron to neuron synapse), GO:0014069 (postsynaptic density), GO:0032279 (asymmetric synapse)