Protein: 60 kDa heat shock protein, mitochondrial (EC 5.6.1.7) (60 kDa chaperonin) (Chaperonin 60) (CPN60) (HSP-65) (Heat shock protein 60) (HSP-60) (Hsp60) (Mitochondrial matrix protein P1) (P63039)
Organism: Rattus norvegicus (Rat)
Function: Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.
Subcellular location: Mitochondrion matrix {ECO:0000250|UniProtKB:P10809}.
Sequence: MLRLPTVLRQMRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKDIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLNLNLEDVQAHDLGKVGEVIVTKDDAMLLKGKGDKAHIEKRIQEITEQLDITTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLKPANEDQKIGIEIIKRALKIPAMTIAKNAGVEGSLIVEKILQSSSEVGYDAMLGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEAVVTEIPKEEKDPGMGAMGGMGGGMGGGMF
InterPro domains: - IPR027410: TCP-1-like chaperonin intermediate domain superfamily (homologous_superfamily) [161-435]
- IPR018370: Chaperonin Cpn60, conserved site (conserved_site) [430-441]
- IPR027409: GroEL-like apical domain superfamily (homologous_superfamily) [208-401]
- IPR002423: Chaperonin Cpn60/GroEL/TCP-1 family (family) [47-548]
- IPR027413: GroEL-like equatorial domain superfamily (homologous_superfamily) [30-547]
- IPR001844: Chaperonin Cpn60/GroEL (family) [23-572]
Interaction partners: - Toll-like receptor 4
- Stress-70 protein, mitochondrial
- 10 kDa heat shock protein, mitochondrial
- Toll-like receptor 2
- 10 kDa heat shock protein, mitochondrial
- Heat shock 70 kDa protein 4
- T-complex protein 1 subunit alpha
- Endoplasmic reticulum chaperone BiP
- Monocyte differentiation antigen CD14
- ATP-dependent Clp protease proteolytic subunit
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0044877 (protein-containing complex binding), GO:0005515 (protein binding), GO:0050839 (cell adhesion molecule binding), GO:0019899 (enzyme binding), GO:0005178 (integrin binding), GO:0005102 (signaling receptor binding), GO:0019900 (kinase binding), GO:0019901 (protein kinase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0002376 (immune system process), GO:0009987 (cellular process), GO:0009628 (response to abiotic stimulus), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0051240 (positive regulation of multicellular organismal process), GO:0002684 (positive regulation of immune system process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0051716 (cellular response to stimulus), GO:0002682 (regulation of immune system process), GO:0051239 (regulation of multicellular organismal process), GO:0048522 (positive regulation of cellular process), GO:0002696 (positive regulation of leukocyte activation), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0009410 (response to xenobiotic stimulus), GO:0031325 (positive regulation of cellular metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0002694 (regulation of leukocyte activation), GO:0080090 (regulation of primary metabolic process), GO:0050865 (regulation of cell activation), GO:0010033 (response to organic substance), GO:0009891 (positive regulation of biosynthetic process), GO:0050867 (positive regulation of cell activation), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0034097 (response to cytokine), GO:0051254 (positive regulation of RNA metabolic process), GO:0043030 (regulation of macrophage activation), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0010468 (regulation of gene expression), GO:1902680 (positive regulation of RNA biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0071345 (cellular response to cytokine stimulus), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0045944 (positive regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0044297 (cell body), GO:0098793 (presynapse), GO:0036477 (somatodendritic compartment), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0012505 (endomembrane system), GO:0043025 (neuronal cell body), GO:0005739 (mitochondrion), GO:0098590 (plasma membrane region), GO:0098857 (membrane microdomain), GO:0120025 (plasma membrane bounded cell projection), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0043005 (neuron projection), GO:0045121 (membrane raft), GO:0043231 (intracellular membrane-bounded organelle)