Protein: RNA polymerase II C-terminal domain phosphatase-like 2 (FCP-like 2) (EC 3.1.3.16) (ASI1-immunoprecipitated protein 4) (Carboxyl-terminal phosphatase-like 2) (AtCPL2) (CTD phosphatase-like 2) (Q5YDB5)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Processively dephosphorylates 'Ser-5' but not 'Ser-2' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II. Together with CPL1, required for male gametes fertility. Multifunctional regulator that modulates plant growth, stress, and phytohormones responses. Positive transcription regulator of genes involved in high salinity resistance and auxin mediated signaling pathway.
Subcellular location: Nucleus {ECO:0000269|PubMed:15388846}. Cytoplasm {ECO:0000269|PubMed:15388846}.
Sequence: MNRLGHKSVVYHGDLRLGELDVNHVSSSHEFRFPNDEIRIHHLSPAGERCPPLAILQTIASFAVRCKLESSAPVKSQELMHLHAVCFHELKTAVVMLGDEEIHLVAMPSKEKKFPCFWCFSVPSGLYDSCLRMLNTRCLSIVFDLDETLIVANTMKSFEDRIEALKSWISREMDPVRINGMSAELKRYMDDRMLLKQYIDNDYAFDNGVLLKAQPEEVRPTSDGQEKVCRPVIRLPEKNTVLTRIKPEIRDTSVLVKLRPAWEELRSYLTAKTRKRFEVYVCTMAERDYALEMWRLLDPEAHLISLKELRDRIVCVKPDAKKSLLSVFNGGICHPKMAMVIDDRMKVWEDKDQPRVHVVSAYLPYYAPQAETALVVPHLCVARNVACNVRGYFFKEFDESLMSSISLVYYEDDVENLPPSPDVSNYVVIEDPGFASNGNINAPPINEGMCGGEVERRLNQAAAADHSTLPATSNAEQKPETPKPQIAVIPNNASTATAAALLPSHKPSLLGAPRRDGFTFSDGGRPLMMRPGVDIRNQNFNQPPILAKIPMQPPSSSMHSPGGWLVDDENRPSFPGRPSGLYPSQFPHGTPGSAPVGPFAHPSHLRSEEVAMDDDLKRQNPSRQTTEGGISQNHLVSNGREHHTDGGKSNGGQSHLFVSALQEIGRRCGSKVEFRTVISTNKELQFSVEVLFTGEKIGIGMAKTKKDAHQQAAENALRSLAEKYVAHVAPLARETEKGPENDNGFLWESSEDVSNKGLEEEAPKENISEL
InterPro domains: - IPR039189: CTD phosphatase Fcp1 (family) [35-420]
- IPR014720: Double-stranded RNA-binding domain (domain) [656-722]
- IPR036412: HAD-like superfamily (homologous_superfamily) [127-391]
- IPR023214: HAD superfamily (homologous_superfamily) [128-384]
- IPR004274: FCP1 homology domain (domain) [134-385]
Interaction partners: - RNA-binding KH domain-containing protein RCF3
- Transcription initiation factor IIF subunit alpha
- Protein PARALOG OF AIPP2
- SCP1-like small phosphatase 4b
- Tryptophan/tyrosine permease
- Probable magnesium transporter NIPA4
- Double-stranded RNA-binding protein 1
- acylphosphatase
- DNA-directed RNA polymerase II subunit 4
- RNA polymerase II C-terminal domain phosphatase-like 5
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0042578 (phosphoric ester hydrolase activity), GO:0016791 (phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0040007 (growth), GO:0048589 (developmental growth), GO:0009628 (response to abiotic stimulus), GO:0009719 (response to endogenous stimulus), GO:0006950 (response to stress), GO:0042221 (response to chemical), GO:0006970 (response to osmotic stress), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:0009733 (response to auxin)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)