Protein: Enoyl-[acyl-carrier-protein] reductase, mitochondrial (EC 1.3.1.104) (2-enoyl thioester reductase) (Q10488)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Required for respiration and the maintenance of the mitochondrial compartment.
Subcellular location: Mitochondrion matrix {ECO:0000250|UniProtKB:P38071}.
Sequence: MSFFKTAVRRFSSTSITRGMAKAIAYSEYGNPKEVLRAVSYNVPKCSKNQVNVRFLASPINPSDINQIQGVYPSKPPFTNDVCSSKPSAVAGNEGLVEVVDVGDQFKGTFSPGQWAILGSVNLGSWRTEMNIDGRSLVPVDKSAFPSIAEAATLSVNPCTAYCLLQHVVQLNKGDWFIQDGANSMVGIATIQLAKHFGYKSINVVRNRPDIEKLKEQLKSLGATIVITDEELMDRKTMKQKVPEWIQGGEVKLGIDCVSGRVAAEMAKYMSKGATMATFGGMSRQPLPVPVSLLIFKNLKFHGFWVTKWKSEHPEEFLKIIHKVEDFYRNGTLKTVNTELVSLKEDADEKTFLDTFLNAIEGHGKKIIKFEH
InterPro domains: - IPR036291: NAD(P)-binding domain superfamily (homologous_superfamily) [148-331]
- IPR020843: Enoylreductase domain (domain) [30-368]
- IPR013149: Alcohol dehydrogenase-like, C-terminal (domain) [186-319]
- IPR011032: GroES-like superfamily (homologous_superfamily) [20-191]
- IPR051034: Mitochondrial Enoyl-ACP Reductase (family) [18-368]
Interaction partners: - Putative 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial
- Hydroxyacyl-thioester dehydratase type 2, mitochondrial
- Malonyl CoA-acyl carrier protein transacylase, mitochondrial
- Palmitoyl-protein thioesterase-dolichyl pyrophosphate phosphatase fusion 1
- Fatty acid synthase subunit alpha
- Fatty acid synthase subunit beta dehydratase; Enoyl-[acyl-carrier-protein] reductase; [Acyl-carrier-protein] acetyltransferase; [Acyl-carrier-protein] malonyltransferase; S-acyl fatty acid synthase thioesterase]
- Probable octanoyltransferase
- Uncharacterized protein C22H10.11c
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016627 (oxidoreductase activity, acting on the CH-CH group of donors), GO:0016628 (oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044281 (small molecule metabolic process), GO:0044237 (cellular metabolic process), GO:0044238 (primary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0006629 (lipid metabolic process), GO:0006082 (organic acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:0006631 (fatty acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)