Protein: Growth factor receptor-bound protein 14 (GRB14 adapter protein) (Q14449)
Organism: Homo sapiens (Human)
Function: Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:15210700, ECO:0000269|PubMed:19648926}. Endosome membrane {ECO:0000269|PubMed:15210700, ECO:0000269|PubMed:19648926}; Peripheral membrane protein {ECO:0000269|PubMed:15210700, ECO:0000269|PubMed:19648926}. Note=Upon insulin stimulation, translocates to the plasma membrane. {ECO:0000269|PubMed:15210700, ECO:0000269|PubMed:19648926}.
Sequence: MTTSLQDGQSAASRAAARDSPLAAQVCGAAQGRGDAHDLAPAPWLHARALLPLPDGTRGCAADRRKKKDLDVPEMPSIPNPFPELCCSPFTSVLSADLFPKANSRKKQVIKVYSEDETSRALDVPSDITARDVCQLLILKNHYIDDHSWTLFEHLPHIGVERTIEDHELVIEVLSNWGIEEENKLYFRKNYAKYEFFKNPMYFFPEHMVSFATETNGEISPTQILQMFLSSSTYPEIHGFLHAKEQGKKSWKKIYFFLRRSGLYFSTKGTSKEPRHLQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKPNKAGGPRDLKMLCAEEEQSRTCWVTAIRLLKYGMQLYQNYMHPYQGRSGCSSQSISPMRSISENSLVAMDFSGQKSRVIENPTEALSVAVEEGLAWRKKGCLRLGTHGSPTASSQSSATNMAIHRSQPWFHHKISRDEAQRLIIQQGLVDGVFLVRDSQSNPKTFVLSMSHGQKIKHFQIIPVEDDGEMFHTLDDGHTRFTDLIQLVEFYQLNKGVLPCKLKHYCARIAL
InterPro domains: - IPR001849: Pleckstrin homology domain (domain) [234-344]
- IPR015042: BPS (Between PH and SH2) domain (domain) [369-414]
- IPR039664: GRB/APBB1IP (family) [101-540]
- IPR029071: Ubiquitin-like domain superfamily (homologous_superfamily) [102-198]
- IPR000159: Ras-associating domain (domain) [106-192]
- IPR039665: APBB1IP, PH domain (domain) [230-351]
- IPR036860: SH2 domain superfamily (homologous_superfamily) [421-540]
- IPR035034: Grb14, SH2 domain (domain) [433-540]
- IPR000980: SH2 domain (domain) [437-535]
- IPR011993: PH-like domain superfamily (homologous_superfamily) [195-356]
Interaction partners: - Insulin receptor
- Fibroblast growth factor receptor 1
- GTPase HRas
- Poly [ADP-ribose] polymerase tankyrase-2
- Epidermal growth factor receptor
- Poly [ADP-ribose] polymerase tankyrase-1
- Cordon-bleu protein-like 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0008289 (lipid binding), GO:0005515 (protein binding), GO:0042802 (identical protein binding), GO:0019899 (enzyme binding), GO:0005543 (phospholipid binding), GO:0019900 (kinase binding), GO:0035091 (phosphatidylinositol binding), GO:0019901 (protein kinase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0065007 (biological regulation), GO:0051716 (cellular response to stimulus), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0050794 (regulation of cellular process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)