Protein: DNA polymerase iota (EC 2.7.7.7) (Eta2) (RAD30 homolog B) (Q9UNA4)
Organism: Homo sapiens (Human)
Function: Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
Subcellular location: Nucleus {ECO:0000269|PubMed:12606586}. Note=Binding to ubiquitin mediates localization to replication forks after UV-induced DNA damage. {ECO:0000250|UniProtKB:Q6R3M4}.
Sequence: MEKLGVEPEEEGGGDDDEEDAEAWAMELADVGAAASSQGVHDQVLPTPNASSRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGFDENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLVGSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGISVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELLASLLNRVCQDGRKPHTVRLIIRRYSSEKHYGRESRQCPIPSHVIQKLGTGNYDVMTPMVDILMKLFRNMVNVKMPFHLTLLSVCFCNLKALNTAKKGLIDYYLMPSLSTTSRSGKHSFKMKDTHMEDFPKDKETNRDFLPSGRIESTRTRESPLDTTNFSKEKDINEFPLCSLPEGVDQEVFKQLPVDIQEEILSGKSREKFQGKGSVSCPLHASRGVLSFFSKKQMQDIPINPRDHLSSSKQVSSVSPCEPGTSGFNSSSSSYMSSQKDYSYYLDNRLKDERISQGPKEPQGFHFTNSNPAVSAFHSFPNLQSEQLFSRNHTTDSHKQTVATDSHEGLTENREPDSVDEKITFPSDIDPQVFYELPEAVQKELLAEWKRAGSDFHIGHK
InterPro domains: - IPR017961: DNA polymerase, Y-family, little finger domain (domain) [324-439]
- IPR043502: DNA/RNA polymerase superfamily (homologous_superfamily) [52-323]
- IPR001126: UmuC domain (domain) [55-268]
- IPR043128: Reverse transcriptase/Diguanylate cyclase domain (homologous_superfamily) [120-246]
- IPR036775: DNA polymerase, Y-family, little finger domain superfamily (homologous_superfamily) [324-445]
- IPR053848: DNA polymerase IV/DNA polymerase iota-like, thumb domain (domain) [262-314]
- IPR025527: HUWE1/REV1, ubiquitin-binding motif (conserved_site) [521-731]
Interaction partners: - Proliferating cell nuclear antigen
- RNA polymerase I-specific transcription initiation factor RRN3
- Polyubiquitin-C
- DNA repair protein REV1
- Ubiquitin-ribosomal protein eS31 fusion protein
- Polyubiquitin-B
- Nucleolar transcription factor 1
- Cellular tumor antigen p53
- NAD-dependent protein deacetylase sirtuin-7
- DNA polymerase zeta catalytic subunit
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:0016740 (transferase activity), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0140097 (catalytic activity, acting on DNA), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0003677 (DNA binding), GO:0034061 (DNA polymerase activity), GO:0016779 (nucleotidyltransferase activity), GO:0003684 (damaged DNA binding), GO:0003887 (DNA-directed DNA polymerase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009628 (response to abiotic stimulus), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0104004 (cellular response to environmental stimulus), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0009314 (response to radiation), GO:0033554 (cellular response to stress), GO:0071214 (cellular response to abiotic stimulus), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0071478 (cellular response to radiation), GO:0009059 (macromolecule biosynthetic process), GO:0009416 (response to light stimulus), GO:1901362 (organic cyclic compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0006259 (DNA metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0071897 (DNA biosynthetic process), GO:0071482 (cellular response to light stimulus), GO:0009411 (response to UV), GO:0006281 (DNA repair), GO:0000731 (DNA synthesis involved in DNA repair), GO:0010225 (response to UV-C), GO:0034644 (cellular response to UV), GO:0006301 (postreplication repair), GO:0019985 (translesion synthesis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0016604 (nuclear body), GO:0005654 (nucleoplasm), GO:0099080 (supramolecular complex), GO:0035770 (ribonucleoprotein granule), GO:0036464 (cytoplasmic ribonucleoprotein granule), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0016607 (nuclear speck), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)