Protein: Protein argonaute-3 (Argonaute3) (hAgo3) (EC 3.1.26.n2) (Argonaute RISC catalytic component 3) (Eukaryotic translation initiation factor 2C 3) (eIF-2C 3) (eIF2C 3) (Q9H9G7)
Organism: Homo sapiens (Human)
Function: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity.
Subcellular location: Cytoplasm, P-body {ECO:0000255|HAMAP-Rule:MF_03032, ECO:0000269|PubMed:16081698}.
Sequence: MEIGSAGPAGAQPLLMVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSIKFVSRVSWHLLHEVLTGRTLPEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA
InterPro domains: - IPR032472: Argonaute linker 2 domain (domain) [375-421]
- IPR012337: Ribonuclease H-like superfamily (homologous_superfamily) [504-820]
- IPR032473: Protein argonaute, Mid domain (domain) [430-511]
- IPR036397: Ribonuclease H superfamily (homologous_superfamily) [583-860]
- IPR003100: PAZ domain (domain) [230-371]
- IPR003165: Piwi domain (domain) [518-819]
- IPR014811: Argonaute, linker 1 domain (domain) [176-228]
- IPR032474: Protein argonaute, N-terminal (domain) [28-167]
- IPR028603: Protein argonaute-3 (family) [1-860]
- IPR036085: PAZ domain superfamily (homologous_superfamily) [223-389]
- IPR045246: Argonaute-like, PIWI domain (domain) [393-818]
Interaction partners: - Protein argonaute-2
- Endoribonuclease Dicer
- Protein argonaute-1
- Trinucleotide repeat-containing gene 6A protein
- Trinucleotide repeat-containing gene 6C protein
- Trinucleotide repeat-containing gene 6B protein
- RISC-loading complex subunit TARBP2
- Protein argonaute-4
- Ribonuclease 3
- Probable ATP-dependent RNA helicase DDX6
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0003725 (double-stranded RNA binding), GO:0003727 (single-stranded RNA binding), GO:0061980 (regulatory RNA binding), GO:0035198 (miRNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0048583 (regulation of response to stimulus), GO:0009893 (positive regulation of metabolic process), GO:0023051 (regulation of signaling), GO:0023056 (positive regulation of signaling), GO:0048584 (positive regulation of response to stimulus), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0048522 (positive regulation of cellular process), GO:0009967 (positive regulation of signal transduction), GO:0060255 (regulation of macromolecule metabolic process), GO:0010647 (positive regulation of cell communication), GO:0009966 (regulation of signal transduction), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0010646 (regulation of cell communication), GO:0010628 (positive regulation of gene expression), GO:1902533 (positive regulation of intracellular signal transduction), GO:1902531 (regulation of intracellular signal transduction), GO:0010468 (regulation of gene expression), GO:1901222 (regulation of NIK/NF-kappaB signaling), GO:1901224 (positive regulation of NIK/NF-kappaB signaling)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:1990904 (ribonucleoprotein complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0140535 (intracellular protein-containing complex), GO:0099080 (supramolecular complex), GO:0035770 (ribonucleoprotein granule), GO:0036464 (cytoplasmic ribonucleoprotein granule), GO:0043229 (intracellular organelle), GO:1905348 (endonuclease complex), GO:0031332 (RNAi effector complex), GO:0043228 (non-membrane-bounded organelle), GO:0016442 (RISC complex), GO:1902555 (endoribonuclease complex), GO:0043232 (intracellular non-membrane-bounded organelle)