Protein: Dihydrolipoyl dehydrogenase, mitochondrial (EC 1.8.1.4) (Dihydrolipoamide dehydrogenase) (O08749)
Organism: Mus musculus (Mouse)
Function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex) (By similarity). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion (By similarity). A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (By similarity). In monomeric form may have additional moonlighting function as serine protease. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction (By similarity).
Subcellular location: Mitochondrion matrix {ECO:0000305|PubMed:36854377, ECO:0000305|PubMed:9169128}. Nucleus {ECO:0000250|UniProtKB:P09622}. Cell projection, cilium, flagellum {ECO:0000250|UniProtKB:Q811C4}. Cytoplasmic vesicle, secretory vesicle, acrosome {ECO:0000269|PubMed:15888450}. Note=Mainly localizes in the mitochondrion. A small fraction localizes to the nucleus, where the 2-oxoglutarate dehydrogenase complex is required for histone succinylation. {ECO:0000250|UniProtKB:P09622}.
Sequence: MQSWSRVYRSLAKKGHFNRISHGLQGVSSVPLRTYADQPIEADVTVIGSGPGGYVAAIKSAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEIPEVRLNLEKMMEQKHSAVKALTGGIAHLFKQNKVVHVNGFGKITGKNQVTATKADGSTQVIDTKNILVATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKLVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGIGIDMEISKNFQRILQRQGFKFKLNTKVTGATKKSDGKIDVSVEAASGGKAEVITCDVLLVCIGRRPFTQNLGLEELGIELDPKGRIPVNNRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEFKIGKFPFAANSRAKTNADTDGMVKILGHKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAAAFGKPINF
InterPro domains: - IPR004099: Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain (domain) [389-497]
- IPR012999: Pyridine nucleotide-disulphide oxidoreductase, class I, active site (active_site) [77-87]
- IPR050151: Class-I pyridine nucleotide-disulfide oxidoreductase (family) [41-504]
- IPR023753: FAD/NAD(P)-binding domain (domain) [43-370]
- IPR006258: Dihydrolipoamide dehydrogenase (family) [42-508]
- IPR001100: Pyridine nucleotide-disulphide oxidoreductase, class I (family) [22-508]
- IPR036188: FAD/NAD(P)-binding domain superfamily (homologous_superfamily) [39-379]
- IPR016156: FAD/NAD-linked reductase, dimerisation domain superfamily (homologous_superfamily) [385-509]
Interaction partners: - Pyruvate dehydrogenase protein X component, mitochondrial
- 2-oxoglutarate dehydrogenase complex component E1
- Pyruvate dehydrogenase E1 component subunit beta, mitochondrial
- Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
- Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
- Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial
- Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
- 2-oxoglutarate dehydrogenase-like, mitochondrial
- Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
- 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016667 (oxidoreductase activity, acting on a sulfur group of donors), GO:0016668 (oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor)
Biological Process (BP): GO:0008150 (biological process), GO:0032501 (multicellular organismal process), GO:0003008 (system process), GO:0007610 (behavior), GO:0007611 (learning or memory), GO:0050877 (nervous system process), GO:0007613 (memory), GO:0050890 (cognition), GO:0007616 (long-term memory)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098798 (mitochondrial protein-containing complex), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005739 (mitochondrion), GO:0043233 (organelle lumen), GO:1990234 (transferase complex), GO:0043229 (intracellular organelle), GO:1990204 (oxidoreductase complex), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005759 (mitochondrial matrix), GO:0070013 (intracellular organelle lumen)