Protein: Lhr helicase/uracil glycosylase (P30015)
Organism: Escherichia coli (strain K12)
Function: A 3'-5' helicase probably involved in DNA repair. Translocates in an ATP-dependent manner 3'-to-5' on single-stranded (ss)DNA, unwinding any encountered duplex nucleic acid. An RNA:DNA hybrid with a 3'-ssDNA loading strand is a 4.5-fold better helicase substrate than 3'-tailed double-stranded (ds)DNA; substrates where the helicase loads on a 3'-ssRNA tail (DNA:RNA and RNA:RNA) are not unwound. Unlike its M.smegmatis counterpart, the ATPase is not ssDNA-dependent. Forms a clamp around the ssDNA loading strand (By similarity)
Sequence: MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQPQTWHVAARSEHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQRNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYLMLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVRSASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIEEQRNATGIVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWAVAIAGRIHALWGADASVVASDDGIVARIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEYAAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEALWALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPNLAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSMEDSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNLLPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTTALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG
InterPro domains: - IPR011545: DEAD/DEAH-box helicase domain (domain) [31-218]
- IPR001650: Helicase, C-terminal domain-like (domain) [284-462]
- IPR055369: Large helicase-related protein, winged-helix domain 2 (domain) [912-1016]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [26-249]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [13-429]
- IPR003593: AAA+ ATPase domain (domain) [43-404]
- IPR055368: Large helicase-related protein, winged-helix domain 3 (domain) [1099-1201]
- IPR052511: ATP-dependent Helicase (family) [13-917]
- IPR045628: Helicase Lhr-like, winged helix domain (domain) [446-616]
- IPR013701: Lhr-like, DEAD/H associated domain (domain) [679-867]
- IPR055367: Large helicase-related protein, winged-helix domain 4 (domain) [1334-1416]
Interaction partners: - Ribonuclease T
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0004386 (helicase activity), GO:0008094 (ATP-dependent activity, acting on DNA), GO:0005515 (protein binding), GO:0140097 (catalytic activity, acting on DNA), GO:0003678 (DNA helicase activity), GO:0043138 (3'-5' DNA helicase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009628 (response to abiotic stimulus), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0051716 (cellular response to stimulus), GO:0071840 (cellular component organization or biogenesis), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0009314 (response to radiation), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0006259 (DNA metabolic process), GO:0006996 (organelle organization), GO:0010212 (response to ionizing radiation), GO:0010165 (response to X-ray), GO:0006281 (DNA repair), GO:0051276 (chromosome organization), GO:0006289 (nucleotide-excision repair)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005829 (cytosol), GO:1902494 (catalytic complex), GO:0005622 (intracellular anatomical structure), GO:0005737 (cytoplasm), GO:0140535 (intracellular protein-containing complex), GO:1905348 (endonuclease complex), GO:1905347 (endodeoxyribonuclease complex)