Protein: Brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2) (ARF guanine-nucleotide exchange factor BIG2) (Q9LZX8)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Activates the ARF proteins by exchanging bound GDP for free GTP. Plays a role in vesicular protein sorting (By similarity).
Subcellular location: Cytoplasm, cytosol {ECO:0000250}. Membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Note=Soluble and partially membrane-bound. {ECO:0000250}.
Sequence: MASSEADSRLSRVVTPALEKIVKNASWRKHSKLANECKAVIERLNSLQKSPPPSSSAATDSESESSVPGPLNDGGSIEYSLADSELIFSPLINACGTGLAKIIEPAIDCIQKLIAHGYIRGESDPSGGAESLLLFKLIDSVCKCHDLGDESIELPVLKTLLSAINSISLRIHGKCLLLVVRTCYDIYLGSKNVVNQTTAKASLIQILVIVFRRMEADSSTVPIQPIVVAELMEPLEKSDADGTMTQFVQGFITKIMQDIDGVLNPTMSGSGSGSGSGGQDGAYGTTTVETTNPTDLLDSTDKDMLDAKYWEISMYKSALEGRKGELTDGDAERDDDLEVQIENKLRRDACLVFRALCKLSMKAPPKESSADPQSMRGKILALELLKILLENAGAVFRTSEKFSADIKQFLCLSLLKNSASTLMIIFQLSCSIFISLVARFRAGLKAEIGVFFPMIVLRVVENVAQPNFQQKMIVLRFLDKLCLDSQILVDIFLNYDCDVNSSNIFERMVNGLLKTAQGVPPGTATTLMPPQEAAMKLEAMKCLVAILKSMGDWLNKQLRLPVSNSLNKSDVIEIDLGPGSPQLANGNADESADGSDTYSESSGGTSDALAIEQRRAYKLELQEGISLFNRKPTKGIEFLINAGKVGESPEEIAGFLKDASGLNKTLIGDYLGEREDLALKVMHAYVDSFDFRGMEFDEAIRTFLEGFRLPGEAQKIDRIMEKFAERYCKCNPKVFTSADSAYVLAYSVIMLNTDAHNPMVKNKMSADDFIRNNRGIDDGKDLPADYMRSLYERITKHEIKMKEDDLRLQQKQYANSNRMLGLDGILNIVIRKQWGDSYAETSDDLMKHMQEQFKEKARKSESTYYAATDVVILRFMIEACWAPMLAAFSVPLDQSDDLIVINICLEGFHHAIHATSLMSMKTHRDAFVTSLAKFTSLHSPADIKQRNIEAIKAILRLADEEGNYLQDAWEHILTCVSRFEQLHLLGEGAPPDATFFASKQNESEKSKQPKQYILPVLKRKGPGKSQYAATGVLRGSYDSMSLGGKGSKNVRQEQMSSIVSNLNLLEQVGEMNQVFSQSQKLNSEAIIDFVKALCKVSMDELRSPSNPRVFSLTKIVEIAHYNMNRIRLVWSSIWQVLSGFFVTIGCSENLSIAIFAMDSLRQLSMKFLEREELANYNFQNEFMTPFVIVMRRSNDVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAAYDDHKNIVFLSFEIIEKIIREYFPYITETETTTFTDCVNCLVAFTNNRFSKDISLSSIAFLRYCATKLAEGDLNSPSTNKYKGTSGKIPQSSLHSGKSGKQENGEIVNNNHLYFWFPLLSGLSELSFDPRPEIRKSALQIMFDTLRNHGHLFSLPLWEKVFESVLFPIFDYVRHSIDPSGEDESADQGSSGGEVDELDHDAWLYETCTLALQLVVDLFVKFYTTVNPLLEKVLMLLVSFIKRPHQSLAGIGIAAFVRLMSDADGLFSEEKWLEVVSALKEAAKTTCPDFSYFLSEEYVARSQRSALNIQNSNAESAAPTATDGNEESQRTATHLYAAISDAKCRAAVQLLLIQAVMEIYNMYRPQLSAKNTLVLVDALHGVALHAHGINSNTILRSRLQELGPMTQMQDPPLLRLENESYQICLTFLQNLVADKTKKEEEEEEEEIESLLVNICQEVLNFYIETSSSAKKLQSESSRASEYRWRIPLGSGKRRELSARAPLIVATLQAMCTLDEASFEKNLKCLFPLLANLISCEHGSNEVQTALADMLGLSVGPVLLQWC
InterPro domains: - IPR016024: Armadillo-type fold (homologous_superfamily) [378-1782]
- IPR000904: Sec7 domain (domain) [610-799]
- IPR015403: Mon2/Sec7/BIG1-like, HDS (domain) [1166-1248]
- IPR035999: Sec7 domain superfamily (homologous_superfamily) [614-805]
- IPR023394: Sec7, C-terminal domain superfamily (homologous_superfamily) [691-807]
- IPR032691: Mon2/Sec7/BIG1-like, HUS domain (domain) [346-504]
- IPR032817: Mon2, C-terminal (domain) [1345-1421]
- IPR032629: Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain (domain) [6-217]
- IPR046455: Sec7/BIG1-like, C-terminal domain (domain) [1578-1781]
Interaction partners: - Coatomer subunit gamma
- V-type proton ATPase subunit a1
- ADP-ribosylation factor 2-B
- Pentatricopeptide repeat-containing protein At3g49170, chloroplastic
- Phosphatidylinositol 4-kinase gamma 6
- MACPF domain-containing protein CAD1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0002376 (immune system process), GO:0009987 (cellular process), GO:0009605 (response to external stimulus), GO:0065008 (regulation of biological quality), GO:0050793 (regulation of developmental process), GO:0051641 (cellular localization), GO:0009607 (response to biotic stimulus), GO:0006955 (immune response), GO:0032879 (regulation of localization), GO:0050794 (regulation of cellular process), GO:0051234 (establishment of localization), GO:0006950 (response to stress), GO:0051707 (response to other organism), GO:0033036 (macromolecule localization), GO:0051049 (regulation of transport), GO:0043207 (response to external biotic stimulus), GO:0046907 (intracellular transport), GO:0009617 (response to bacterium), GO:0006810 (transport), GO:0022603 (regulation of anatomical structure morphogenesis), GO:0006952 (defense response), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0060627 (regulation of vesicle-mediated transport), GO:0010817 (regulation of hormone levels), GO:0051128 (regulation of cellular component organization), GO:0045087 (innate immune response), GO:0045184 (establishment of protein localization), GO:0098542 (defense response to other organism), GO:0031338 (regulation of vesicle fusion), GO:1905421 (regulation of plant organ morphogenesis), GO:0006886 (intracellular protein transport), GO:0071705 (nitrogen compound transport), GO:0042742 (defense response to bacterium), GO:0015031 (protein transport), GO:0033043 (regulation of organelle organization), GO:0071702 (organic substance transport), GO:0016192 (vesicle-mediated transport), GO:0008104 (protein localization), GO:2000012 (regulation of auxin polar transport), GO:2000067 (regulation of root morphogenesis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0043226 (organelle), GO:0005622 (intracellular anatomical structure), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031984 (organelle subcompartment), GO:0005794 (Golgi apparatus), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0098791 (Golgi apparatus subcompartment), GO:0043231 (intracellular membrane-bounded organelle), GO:0005802 (trans-Golgi network)