Protein: ATP-dependent helicase FUN30 (EC 3.6.4.12) (P31380)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs, facilitating single-stranded DNA (ssDNA) production by the EXO1 and SGS1 machinery. Promotes gene silencing at heterochromatin by regulating the chromatin structure within or around silent loci. Also required for heterochromatin organization at centromeres.
Subcellular location: Nucleus. Chromosome. Note=Recruited to double-strand breaks (DSBs) sites of DNA damage.
Sequence: MSGSHSNDEDDVVQVPETSSPTKVASSSPLKPTSPTVPDASVASLRSRFTFKPSDPSEGAHTSKPLPSGSPEVALVNLAREFPDFSQTLVQAVFKSNSFNLQSARERLTRLRQQRQNWTWNKNASPKKSETPPPVKKSLPLANTGRLSSIHGNINNKSSKITVAKQKTSIFDRYSNVINQKQYTFELPTNLNIDSEALSKLPVNYNKKRRLVRADQHPIGKSYESSATQLGSAREKLLANRKYGRHANDNDEEEEESMMTDDDDASGDDYTESTPQINLDEQVLQFINDSDIVDLSDLSDTTMHKAQLIASHRPYSSLNAFVNTNFNDKDTEENASNKRKRRAAASANESERLLDKITQSIRGYNAIESVIKKCSSYGDLVTSQMKKWGVQVEGDNSELDLMNLGEDDDDDNDDGNNDNNNSNNNNTAGADATSKEKEDTKAVVEGFDETSAEPTPAPAPAPVERETKRIRNTTKPKVVEDEDDDVDLEAIDDELPQSEHEDDDYEEEDEDYNDEEEDVEYDDGDDDDDDDDEFVATRKNTHVISTTSRNGRKPIVKFFKGKPRLLSPEISLKDYQQTGINWLNLLYQNKMSCILADDMGLGKTCQVISFFAYLKQINEPGPHLVVVPSSTLENWLREFQKFAPALKIEPYYGSLQEREELRDILERNAGKYDVIVTTYNLAAGNKYDVSFLKNRNFNVVVYDEGHMLKNSTSERFAKLMKIRANFRLLLTGTPLQNNLKELMSLLEFIMPNLFISKKESFDAIFKQRAKTTDDNKNHNPLLAQEAITRAKTMMKPFILRRRKDQVLKHLPPKHTHIQYCELNAIQKKIYDKEIQIVLEHKRMIKDGELPKDAKEKSKLQSSSSKNLIMALRKASLHPLLFRNIYNDKIITKMSDAILDEPAYAENGNKEYIKEDMSYMTDFELHKLCCNFPNTLSKYQLHNDEWMQSGKIDALKKLLKTIIVDKQEKVLIFSLFTQVLDILEMVLSTLDYKFLRLDGSTQVNDRQLLIDKFYEDKDIPIFILSTKAGGFGINLVCANNVIIFDQSFNPHDDRQAADRAHRVGQTKEVNITTLITKDSIEEKIHQLAKNKLALDSYISEDKKSQDVLESKVSDMLEDIIYDENSKPKGTKE
InterPro domains: - IPR000330: SNF2, N-terminal (domain) [575-878]
- IPR049730: SNF2/RAD5-like, C-terminal helicase domain (domain) [944-1074]
- IPR038718: SNF2-like, N-terminal domain superfamily (homologous_superfamily) [540-827]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [530-1131]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [568-764]
- IPR001650: Helicase, C-terminal domain-like (domain) [950-1108]
Interaction partners: - Exodeoxyribonuclease 1
- DNA replication ATP-dependent helicase/nuclease DNA2
- ATP-dependent helicase SGS1
- DNA repair protein RAD9
- DNA mismatch repair protein MSH2
- Histone H3
- DNA replication regulator DPB11
- C-1-tetrahydrofolate synthase, mitochondrial
- ISWI chromatin-remodeling complex ATPase ISW1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0140104 (molecular carrier activity), GO:0008094 (ATP-dependent activity, acting on DNA), GO:0140597 (protein carrier chaperone), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0042393 (histone binding), GO:0140030 (modification-dependent protein binding), GO:0140658 (ATP-dependent chromatin remodeler activity), GO:0140097 (catalytic activity, acting on DNA), GO:0140713 (histone chaperone activity), GO:0070577 (lysine-acetylated histone binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0051716 (cellular response to stimulus), GO:0071840 (cellular component organization or biogenesis), GO:0006950 (response to stress), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0006325 (chromatin organization), GO:0006259 (DNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0006338 (chromatin remodeling), GO:0006281 (DNA repair), GO:0006289 (nucleotide-excision repair)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000785 (chromatin), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus), GO:0005694 (chromosome)