Protein: Hyaluronan-binding protein 2 (EC 3.4.21.-) (Factor VII-activating protease) (Factor seven-activating protease) (FSAP) (Hepatocyte growth factor activator-like protein) (Plasma hyaluronan-binding protein) [Cleaved into: Hyaluronan-binding protein 2 50 kDa heavy chain; Hyaluronan-binding protein 2 50 kDa heavy chain alternate form; Hyaluronan-binding protein 2 27 kDa light chain; Hyaluronan-binding protein 2 27 kDa light chain alternate form] (Q14520)
Organism: Homo sapiens (Human)
Function: Cleaves the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro-urokinase) to the active two chain form. Activates coagulation factor VII. May function as a tumor suppressor negatively regulating cell proliferation and cell migration.
Subcellular location: Secreted {ECO:0000269|PubMed:8827452}. Note=Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.
Sequence: MFARMSDLHVLLLMALVGKTACGFSLMSLLESLDPDWTPDQYDYSYEDYNQEENTSSTLTHAENPDWYYTEDQADPCQPNPCEHGGDCLVHGSTFTCSCLAPFSGNKCQKVQNTCKDNPCGRGQCLITQSPPYYRCVCKHPYTGPSCSQVVPVCRPNPCQNGATCSRHKRRSKFTCACPDQFKGKFCEIGSDDCYVGDGYSYRGKMNRTVNQHACLYWNSHLLLQENYNMFMEDAETHGIGEHNFCRNPDADEKPWCFIKVTNDKVKWEYCDVSACSAQDVAYPEESPTEPSTKLPGFDSCGKTEIAERKIKRIYGGFKSTAGKHPWQASLQSSLPLTISMPQGHFCGGALIHPCWVLTAAHCTDIKTRHLKVVLGDQDLKKEEFHEQSFRVEKIFKYSHYNERDEIPHNDIALLKLKPVDGHCALESKYVKTVCLPDGSFPSGSECHISGWGVTETGKGSRQLLDAKVKLIANTLCNSRQLYDHMIDDSMICAGNLQKPGQDTCQGDSGGPLTCEKDGTYYVYGIVSWGLECGKRPGVYTQVTKFLNWIKATIKSESGF
InterPro domains: - IPR001314: Peptidase S1A, chymotrypsin family (family) [348-514]
- IPR043504: Peptidase S1, PA clan, chymotrypsin-like fold (homologous_superfamily) [307-556]
- IPR000001: Kringle (domain) [191-278]
- IPR001881: EGF-like calcium-binding domain (domain) [73-188]
- IPR013806: Kringle-like fold (homologous_superfamily) [174-281]
- IPR018056: Kringle, conserved site (conserved_site) [245-257]
- IPR018114: Serine proteases, trypsin family, histidine active site (active_site) [358-363]
- IPR038178: Kringle superfamily (homologous_superfamily) [194-286]
- IPR033116: Serine proteases, trypsin family, serine active site (active_site) [503-514]
- IPR050127: Serine Proteases (Peptidase S1 Family) (family) [166-556]
- IPR000742: EGF-like domain (domain) [73-188]
- IPR009003: Peptidase S1, PA clan (homologous_superfamily) [297-555]
- IPR001254: Serine proteases, trypsin domain (domain) [313-555]
Interaction partners: - Fibrinogen beta chain
- WD repeat-containing protein 90
- Fibrinogen gamma chain
- Fibrinogen alpha chain
- Heparin cofactor 2
- A-type potassium channel modulatory protein KCNIP1
- Histidine-rich glycoprotein
- Inactive tyrosine-protein kinase 7
- Plasminogen activator inhibitor 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0017171 (serine hydrolase activity), GO:0008233 (peptidase activity), GO:0008236 (serine-type peptidase activity), GO:0004175 (endopeptidase activity), GO:0004252 (serine-type endopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0040011 (locomotion), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0002376 (immune system process), GO:0023052 (signaling), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0048870 (cell motility), GO:0042221 (response to chemical), GO:0042330 (taxis), GO:0006955 (immune response), GO:0051240 (positive regulation of multicellular organismal process), GO:0006807 (nitrogen compound metabolic process), GO:0051239 (regulation of multicellular organismal process), GO:0071704 (organic substance metabolic process), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0009056 (catabolic process), GO:0050900 (leukocyte migration), GO:0009607 (response to biotic stimulus), GO:0009719 (response to endogenous stimulus), GO:0040017 (positive regulation of locomotion), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0050792 (regulation of viral process), GO:0006950 (response to stress), GO:0051707 (response to other organism), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0040012 (regulation of locomotion), GO:0048522 (positive regulation of cellular process), GO:0002250 (adaptive immune response), GO:1901564 (organonitrogen compound metabolic process), GO:0043207 (response to external biotic stimulus), GO:2000145 (regulation of cell motility), GO:0035556 (intracellular signal transduction), GO:0001568 (blood vessel development), GO:0044248 (cellular catabolic process), GO:0048731 (system development), GO:0019538 (protein metabolic process), GO:0070848 (response to growth factor), GO:0006952 (defense response), GO:0071495 (cellular response to endogenous stimulus), GO:0043170 (macromolecule metabolic process), GO:2000147 (positive regulation of cell motility), GO:0045087 (innate immune response), GO:0098542 (defense response to other organism), GO:0016477 (cell migration), GO:0001525 (angiogenesis), GO:0035295 (tube development), GO:0010632 (regulation of epithelial cell migration), GO:0042127 (regulation of cell population proliferation), GO:0009888 (tissue development), GO:0030595 (leukocyte chemotaxis), GO:0070887 (cellular response to chemical stimulus), GO:0009617 (response to bacterium), GO:0006959 (humoral immune response), GO:0007166 (cell surface receptor signaling pathway), GO:1901575 (organic substance catabolic process), GO:0006935 (chemotaxis), GO:0008284 (positive regulation of cell population proliferation), GO:0016477 (cell migration), GO:0035239 (tube morphogenesis), GO:0019730 (antimicrobial humoral response), GO:0097530 (granulocyte migration), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0001944 (vasculature development), GO:0030163 (protein catabolic process), GO:0030334 (regulation of cell migration), GO:2001026 (regulation of endothelial cell chemotaxis), GO:0050678 (regulation of epithelial cell proliferation), GO:0001667 (ameboidal-type cell migration), GO:0050679 (positive regulation of epithelial cell proliferation), GO:0030336 (negative regulation of cell migration), GO:0060326 (cell chemotaxis), GO:0010594 (regulation of endothelial cell migration), GO:0048514 (blood vessel morphogenesis), GO:0072359 (circulatory system development), GO:0010634 (positive regulation of epithelial cell migration), GO:0030335 (positive regulation of cell migration), GO:0060429 (epithelium development), GO:0071363 (cellular response to growth factor stimulus), GO:0001938 (positive regulation of endothelial cell proliferation), GO:0001937 (negative regulation of endothelial cell proliferation), GO:0060627 (regulation of vesicle-mediated transport), GO:0010595 (positive regulation of endothelial cell migration), GO:0043542 (endothelial cell migration), GO:0051603 (proteolysis involved in protein catabolic process), GO:0043535 (regulation of blood vessel endothelial cell migration), GO:0001936 (regulation of endothelial cell proliferation), GO:0003158 (endothelium development), GO:0030203 (glycosaminoglycan metabolic process), GO:0044344 (cellular response to fibroblast growth factor stimulus), GO:0043536 (positive regulation of blood vessel endothelial cell migration), GO:0090049 (regulation of cell migration involved in sprouting angiogenesis), GO:0090050 (positive regulation of cell migration involved in sprouting angiogenesis), GO:1905562 (regulation of vascular endothelial cell proliferation), GO:0031638 (zymogen activation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0071944 (cell periphery), GO:0072562 (blood microparticle), GO:0005615 (extracellular space), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0012505 (endomembrane system), GO:0005576 (extracellular region), GO:0031090 (organelle membrane), GO:0030141 (secretory granule), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043230 (extracellular organelle), GO:0005773 (vacuole), GO:0043233 (organelle lumen), GO:0070062 (extracellular exosome), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0060205 (cytoplasmic vesicle lumen), GO:0000323 (lytic vacuole), GO:0031983 (vesicle lumen), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0034774 (secretory granule lumen), GO:0031091 (platelet alpha granule), GO:0065010 (extracellular membrane-bounded organelle), GO:0042582 (azurophil granule), GO:0005775 (vacuolar lumen), GO:0070013 (intracellular organelle lumen), GO:0031982 (vesicle), GO:0005764 (lysosome), GO:0035578 (azurophil granule lumen), GO:1903561 (extracellular vesicle), GO:0097708 (intracellular vesicle), GO:0031093 (platelet alpha granule lumen), GO:0005766 (primary lysosome)