Protein: Phytochrome-associated serine/threonine-protein phosphatase 1 (AtFyPP1) (EC 3.1.3.16) (Q9SX52)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Catalytic subunit of protein phosphatase 6 (PP6) (Probable). Dephosphorylates phosphorylated phytochromes, with a preference toward Pfr forms. Plays a major role in the photoperiodic control of flowering time in long days by modulating phytochrome signals in flowering time control (By similarity). Involved in the regulation of polar auxin transport in roots. Dephosphorylates directly the auxin efflux carriers PIN1 and PIN2, thus promoting their proper polar localization in root cell plasma membrane. Acts antagonistically with the protein kinase PID to regulate the reversible phosphorylation of PIN and polar targeting, subsequently impacting polar auxin transport and plant development. Involved in the regulation of abscisic acid (ABA) signaling during seed germination and postgermination seedling growth. Functions as negative regulator of ABA signaling through direct dephosphorylation and destabilization of ABI5. Acts antagonistically with the protein kinase SRK2E/SNRK2.6 to regulate ABI5 phosphorylation and ABA responses. Involved in the regulation of phosphorylation status in hypocotyl phototropism. Involved in the negative regulation of photomorphogenesis by controlling the stability and transcriptional activity of PIF3 and PIF4 proteins in the dark, via the regulation of their phosphorylation status.
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:Q9LHE7}.
Sequence: MDLDQWISKVKDGQHLSEDELQLLCEYVKEILIEESNVQPVNSPVTVCGDIHGQFHDLMKLFQTGGHVPETNYIFMGDFVDRGYNSLEVFTILLLLKARHPANITLLRGNHESRQLTQVYGFYDECQRKYGNANAWRYCTDVFDYLTLSAIIDGTVLCVHGGLSPDVRTIDQIRLIERNCEIPHEGPFCDLMWSDPEDIETWAVSPRGAGWLFGSRVTTEFNHINNLDLVCRAHQLVQEGLKYMFQDKGLVTVWSAPNYCYRCGNVASILSFNDNMEREVKFFTETEENNQMRGPRTGVPYFL
InterPro domains: - IPR004843: Calcineurin-like, phosphoesterase domain (domain) [44-237]
- IPR047129: Serine/Threonine protein phosphatase PP2A-like (family) [2-303]
- IPR006186: Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase (domain) [16-287]
- IPR029052: Metallo-dependent phosphatase-like (homologous_superfamily) [1-303]
Interaction partners: - PP2A regulatory subunit TAP46
- Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
- Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform
- Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A gamma isoform
- SIT4 phosphatase-associated family protein
- SIT4 phosphatase-associated family protein
- At1g07990/T6D22_5
- SIT4 phosphatase-associated family protein
- Serine/threonine-protein phosphatase 6 regulatory subunit
- Serine/threonine-protein phosphatase 4 regulatory subunit-like protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0043167 (ion binding), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0004721 (phosphoprotein phosphatase activity), GO:0043169 (cation binding), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0046872 (metal ion binding), GO:0042578 (phosphoric ester hydrolase activity), GO:0004722 (protein serine/threonine phosphatase activity), GO:0046914 (transition metal ion binding), GO:0016791 (phosphatase activity), GO:0008270 (zinc ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0050793 (regulation of developmental process), GO:0051241 (negative regulation of multicellular organismal process), GO:0051239 (regulation of multicellular organismal process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:2000241 (regulation of reproductive process), GO:0051093 (negative regulation of developmental process), GO:2000242 (negative regulation of reproductive process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0048831 (regulation of shoot system development), GO:0048580 (regulation of post-embryonic development), GO:2000026 (regulation of multicellular organismal development), GO:1901564 (organonitrogen compound metabolic process), GO:0009910 (negative regulation of flower development), GO:0019538 (protein metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009909 (regulation of flower development), GO:0006793 (phosphorus metabolic process), GO:0048581 (negative regulation of post-embryonic development), GO:0036211 (protein modification process), GO:0006796 (phosphate-containing compound metabolic process), GO:0043412 (macromolecule modification), GO:0016311 (dephosphorylation), GO:0006470 (protein dephosphorylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:1903293 (phosphatase complex), GO:0043227 (membrane-bounded organelle), GO:0008287 (protein serine/threonine phosphatase complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0000159 (protein phosphatase type 2A complex), GO:0005634 (nucleus)