Protein: Serine/threonine-protein kinase N1 (EC 2.7.11.13) (Protease-activated kinase 1) (PAK-1) (Protein kinase C-like 1) (Protein kinase C-like PKN) (Protein-kinase C-related kinase 1) (Serine-threonine protein kinase N) (Q63433)
Organism: Rattus norvegicus (Rat)
Function: PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14 (By similarity). Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro (By similarity).
Subcellular location: Cytoplasm {ECO:0000269|PubMed:15375078}. Nucleus {ECO:0000250|UniProtKB:Q16512}. Endosome {ECO:0000250|UniProtKB:Q16512}. Cell membrane {ECO:0000269|PubMed:15375078}; Peripheral membrane protein {ECO:0000269|PubMed:15375078}. Cleavage furrow {ECO:0000250|UniProtKB:Q16512}. Midbody {ECO:0000250|UniProtKB:Q16512}. Note=Associates with chromatin in a ligand-dependent manner (By similarity). Localization to endosomes is mediated via its interaction with RHOB. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis (By similarity). Association to the cell membrane is dependent on Ser-377 phosphorylation. {ECO:0000250|UniProtKB:Q16512, ECO:0000269|PubMed:15375078}.
Sequence: MAGDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLKREIRKELKLKEGAENLRRATTDLGRSLAPVELLLRGSARRLDLLHQQLQELHAHVVLPDPTAGSDAPQSLAEGSPVCSSTNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQALQAGQLESQAAPDEAHGDPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLRESLERRLGELPADHPKGRLLREELTAASSAAFSAILPGPFPATHYSTLSKPAPLTGTLEVRVVGCKNLPETIPWSPPPSVGASGTPDSRTPFLSRPARGLYNRSGSLSGRSSLKGEAENSTEVSTVLKLDNTVVGQTAWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPIIERIPRLQRQKKIFSKQQGQTFQRARQMNIDVATWVRLLRRLIPNAVATGSFSPNASPGSEIRSTGDISMEKLNLGADSDSSSQKSPAGLPSTSCSLSSPTHESTTSPELPSETQETPGPGLCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFHSSGELFAIKALKKGDIVARDEVESLMCEKRILATVTRAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAVFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSTERDAEDVKKQPFFRTLDWDALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFRDFDFVAGGY
InterPro domains: - IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [616-927]
- IPR035892: C2 domain superfamily (homologous_superfamily) [324-481]
- IPR037784: Serine/threonine-protein kinase N, C2 domain (domain) [392-479]
- IPR000961: AGC-kinase, C-terminal (domain) [879-946]
- IPR000008: C2 domain (domain) [310-473]
- IPR008271: Serine/threonine-protein kinase, active site (active_site) [740-752]
- IPR011072: HR1 rho-binding domain (domain) [25-284]
- IPR017892: Protein kinase, C-terminal (domain) [899-941]
- IPR017441: Protein kinase, ATP binding site (binding_site) [625-648]
- IPR000719: Protein kinase domain (domain) [619-878]
- IPR037317: Serine/threonine-protein kinase N1, second HR1 domain (domain) [121-192]
- IPR037313: Serine/threonine-protein kinase N, first HR1 domain (domain) [32-98]
- IPR036274: HR1 repeat superfamily (homologous_superfamily) [19-287]
Interaction partners: - Transforming protein RhoA
- RSA-14-44 protein.
- Ras-related C3 botulinum toxin substrate 1
- 3-phosphoinositide-dependent protein kinase 1
- Protein SET
- Serine/threonine-protein kinase N2
- M-phase inducer phosphatase
- Rho-related GTP-binding protein RhoB
- Ras homolog family member C.
- 14-3-3 protein epsilon
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0040012 (regulation of locomotion), GO:0050794 (regulation of cellular process), GO:2000145 (regulation of cell motility), GO:0030334 (regulation of cell migration), GO:0010762 (regulation of fibroblast migration)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0000785 (chromatin), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0012505 (endomembrane system), GO:0030141 (secretory granule), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0043228 (non-membrane-bounded organelle), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0001669 (acrosomal vesicle), GO:0031982 (vesicle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome), GO:0097708 (intracellular vesicle), GO:0005634 (nucleus)