Protein: HBS1-like protein (F1Q5Z6)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: GTPase component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. The Pelota-HBS1L complex recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway
Subcellular location: Cytoplasm
Sequence: MSRHRNVRGYNYDEDFEDDDMYGQSVEDDYCISPATAAQFIYSRQDSRQARHVETVEEAEYEEEEEEMPTSPTMTSTLDSLQQGRLYSCLDQMRTVLGDSIPDSTLTQAALKYDCDPHRALDFILSENTNTQAPSARTNPQLEPNTTAAPQKAKVREAPESRVESEVVPKVARMTVSGKKQTMGFDVRSAEENGGVAPSLRRGPSPEATAVPTTETPSKQSTVTEDSAAPTPTRPSGKSKQQLNIRAELEKRQGGKPLLNLVVIGHVDAGKSTLMGHLLYLLGNVNKRTMHKYEQEAKKAGKASFAYAWVLDETGEERDRGVTMDVGMTKFETDSKVVTLMDAPGHKDFIPNMITGAAQADVAVLVVDASRGEFEAGFEAGGQTREHALLVRSLGVTQLAVAVNKMDQVNWQQERFQEIISKLGHFLKQAGFKDSDVFYVPTSGLSGENLTTKSKVADLTAWYTGPCLVEQIDAFKPPQRSVEKPFRLCVSDVFKDQGSGFCVTGKIEAGYIQTGDKVLAMPPNETCTVKGISLHDEALDWAAAGDHVSLTVTGMDIIKINVGCVFCDPKEPIRACTRFRARILLFNIELPITQGFPVLLHYQTVSEPATIRKLVSVLHKSSGEVLKKKPKCLSKGQNAVVEIQTQRPVAMELYKDYKELGRFMLRYVGSTIAAGVVTEIKE
InterPro domains: - IPR000795: Translational (tr)-type GTP-binding domain (domain) [256-480]
- IPR009000: Translation protein, beta-barrel domain superfamily (homologous_superfamily) [477-572]
- IPR037189: HBS1-like protein, N-terminal domain superfamily (homologous_superfamily) [58-133]
- IPR050100: Translation factor GTPase superfamily members (family) [255-681]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [239-500]
- IPR009001: Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal (homologous_superfamily) [576-681]
- IPR004161: Translation elongation factor EFTu-like, domain 2 (domain) [500-567]
- IPR015033: HBS1-like protein, N-terminal (domain) [58-133]
- IPR054696: GTP-eEF1A, C-terminal domain (domain) [578-678]
Interaction partners: - Protein pelota homolog
- FAU ubiquitin-like and ribosomal protein S30 fusion a
- FAU ubiquitin-like and ribosomal protein S30 fusion b
- Exosome complex component RRP45
- Exosome complex component CSL4
- Exosome complex component RRP4
- 40S ribosomal protein S12
- Exosome complex component 10
- ATP-binding cassette sub-family E member 1
- Small ribosomal subunit protein RACK1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0071704 (organic substance metabolic process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006412 (translation), GO:0009059 (macromolecule biosynthetic process), GO:0006518 (peptide metabolic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0010467 (gene expression), GO:0043043 (peptide biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)