Protein: RecQ-like DNA helicase blm-1 (EC 5.6.2.4) (Bloom syndrome protein homolog) (DNA 3'-5' helicase BLM) (High incidence of males protein 6) (RecQ helicase homolog) (O18017)
Organism: Caenorhabditis elegans
Function: Component of the BTR double Holliday Junction dissolution complex, which is involved in homologous recombination during meiotic double strand break in the germline (Probable). Stabilizes and positively regulates the localization of the BTR double Holliday Junction dissolution complex component rmh-1 at nuclear foci during meiotic recombination. Participates in DNA replication and repair (By similarity). Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction (By similarity). Negatively regulates sister chromatid exchange (SCE).
Subcellular location: Nucleus {ECO:0000269|PubMed:27011106, ECO:0000269|PubMed:34252074}. Chromosome {ECO:0000269|PubMed:27011106}. Note=Localizes on chromosomes during pachytene in meiotic prophase I in oocytes. Co-localizes with rmh-1 at mid-pachytene and late-pachytene in meiotic prophase I (PubMed:27011106). Localization at nuclear foci is dependent on rmh-1 during meiotic recombination (PubMed:27011106). {ECO:0000269|PubMed:27011106, ECO:0000269|PubMed:34252074}.
Sequence: MIKNREIEVAPPRRTIQFGGYTFVEPDLNFKAPIFSCCGSIRDPSCEEREEEYIDNGHDEEPPVEVNRIQESTSFDEPVSSPPRYRPSENPGPSSSSYEPGHYSFNEYQQFPSRPQKRLVDPPIVDLDEEPPIVDLDDSFDNFHVGSTSEEVVSGDIAPEEEEEEGHDSFDDFESVPAQPPSKNTLASLQKSDSEIALNQQRHDMHGRFRGFLQDDSEEFSDEVGLLGADMNKELYDTLKSKFGFNQFRHRQKQCILSTLMGHDTFVLMPTGAGKSLCYQLPAVILPGVTVVVSPLRSLIEDQKMKMKELGIGCEALTADLGAPAQEKIYAELGSGNPSIKLLYVTPEKISASGRLNSVFFDLHRRGLLARFVIDEAHCVSQWGHDFRPDYTKLSSLREKYANPPVPIIALTATATPKIVTDARDHLKMQNSKLFISSFVRDNLKYDLIPKAARSLINVVEKMKQLYPGKSGIVYCLSRKECETVQMMLTKAGLSAEVYHAGLNDNLRVSVQRSWIANKFDVICATIAFGMGIDKPDVRFVIHYSLPKSIEGYYQETGRAGRDGMPSYCLMLYSYHDSIRLRRMIEEGNTTTGVRSMHLNNVLQVVAYCENVSVCRRKMLVEHFGEVYDEQSCRNSKTPCDICERQRKNAEAIRLFDVSTDALSILKCLPRMQKATLKYISELYRGALIKKSQEQAMRLGHTKLPFYSKGQGMSEQDALRFVRKLVIEGYIHERLYSVPNQAAAVFAYAELTEAGRDLANGKKTAKVYLHIVTCERKRKNAGLIELSNMNIVSEAQALKERHMVKHGDVFTRCLQDLTHLITAVAESSGLSGPYSIVSREGIEQIAALLPRTNSDLLRIDSMTQIKVTKYGRLIMELLATYWKQVDEREEEEMRNQLDKLKSGEIVMGGFATLQSDPGFPSVPYMKPLGGGGGCRGRGKKRAFSGFSSGRATKKPRATAPSARGKTSGRGGAKPATSLKRNMYPATSM
InterPro domains: - IPR036390: Winged helix DNA-binding domain superfamily (homologous_superfamily) [654-770]
- IPR032284: ATP-dependent DNA helicase RecQ, zinc-binding domain (domain) [575-644]
- IPR002464: DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site (conserved_site) [370-379]
- IPR002121: HRDC domain (domain) [807-888]
- IPR044876: HRDC domain superfamily (homologous_superfamily) [803-892]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [251-418]
- IPR004589: DNA helicase, ATP-dependent, RecQ type (family) [238-696]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [225-648]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [244-449]
- IPR010997: HRDC-like superfamily (homologous_superfamily) [811-883]
- IPR018982: RQC domain (domain) [654-772]
- IPR001650: Helicase, C-terminal domain-like (domain) [458-603]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [649-779]
Interaction partners: - DNA topoisomerase 3
- RecQ-mediated genome instability protein 1
- Exonuclease 1
- Crossover junction endonuclease MUS81
- DNA repair protein RAD51 homolog
- DNA repair endonuclease XPF
- DNA mismatch repair protein S5 domain-containing protein
- Probable replication factor A 73 kDa subunit
- Structure-specific endonuclease subunit SLX1 homolog
- Replication protein A C-terminal domain-containing protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0004386 (helicase activity), GO:0008094 (ATP-dependent activity, acting on DNA), GO:0005515 (protein binding), GO:0140097 (catalytic activity, acting on DNA), GO:0003678 (DNA helicase activity), GO:0043138 (3'-5' DNA helicase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0032501 (multicellular organismal process), GO:0009628 (response to abiotic stimulus), GO:0008340 (determination of adult lifespan), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009314 (response to radiation), GO:0046483 (heterocycle metabolic process), GO:0006259 (DNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010212 (response to ionizing radiation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)