Protein: Eukaryotic initiation factor 4A-III (Q7ZVA6)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions (By similarity). Involved in craniofacial development
Subcellular location: Nucleus. Nucleus speckle. Cytoplasm. Note=Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA
Sequence: MATAVVPVRKRILKEEDMTKIEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFCVSVLQCLDIQVRETQALILAPTRELAGQIQKVLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVCLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVSQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI
InterPro domains: - IPR011545: DEAD/DEAH-box helicase domain (domain) [58-220]
- IPR001650: Helicase, C-terminal domain-like (domain) [245-406]
- IPR014014: RNA helicase, DEAD-box type, Q motif (domain) [33-61]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [16-406]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [52-249]
- IPR000629: ATP-dependent RNA helicase DEAD-box, conserved site (conserved_site) [180-188]
Interaction partners: - RNA-binding protein 8A
- Pre-mRNA-splicing factor CWC22 homolog
- Protein mago nashi homolog
- Protein CASC3
- Nucleolar MIF4G domain-containing protein 1
- RNA-binding protein with serine-rich domain 1
- Regulator of nonsense transcripts 1
- Regulator of nonsense transcripts 3B
- Serine/threonine-protein kinase SMG1
- Neuroguidin
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0003729 (mRNA binding), GO:0003730 (mRNA 3'-UTR binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009628 (response to abiotic stimulus), GO:0071840 (cellular component organization or biogenesis), GO:0006950 (response to stress), GO:0009408 (response to heat), GO:0009266 (response to temperature stimulus), GO:0016043 (cellular component organization), GO:0044085 (cellular component biogenesis), GO:0006996 (organelle organization), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0140694 (non-membrane-bounded organelle assembly), GO:0034063 (stress granule assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)