Protein: Endonuclease 8 2 (DNA glycosylase/AP lyase Nei 2) (EC 3.2.2.-) (DNA-(apurinic or apyrimidinic site) lyase Nei 2) (EC 4.2.99.18) (Endonuclease VIII 2) (P9WNC1)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity). Complements an E.coli nei nth double mutant.
Sequence: MPEGDTVWHTAATLRRHLAGRTLTRCDIRVPRFAAVDLTGEVVDEVISRGKHLFIRTGTASIHSHLQMDGSWRVGNRPVRVDHRARIILEANQQEQAIRVVGVDLGLLEVIDRHNDGAVVAHLGPDLLADDWDPQRAAANLIVAPDRPIAEALLDQRVLAGIGNVYCNELCFVSGVLPTAPVSAVADPRRLVTRARDMLWVNRFRWNRCTTGDTRAGRRLWVYGRAGQGCRRCGTLIAYDTTDERVRYWCPACQR
InterPro domains: - IPR000214: Zinc finger, DNA glycosylase/AP lyase-type (domain) [221-255]
- IPR015886: Formamidopyrimidine-DNA glycosylase, H2TH DNA-binding (domain) [123-211]
- IPR015887: DNA glycosylase/AP lyase, zinc finger domain, DNA-binding site (binding_site) [230-254]
- IPR054878: Endonuclease VIII Nei2 (family) [1-255]
- IPR044090: Nei2, N-terminal (domain) [1-114]
- IPR035937: Formamidopyrimidine-DNA glycosylase, N-terminal (homologous_superfamily) [2-124]
- IPR012319: Formamidopyrimidine-DNA glycosylase, catalytic domain (domain) [1-109]
- IPR010979: Small ribosomal subunit protein uS13-like, H2TH (homologous_superfamily) [122-187]
Interaction partners: - Probable ATP-dependent helicase Lhr
- Uncharacterized formamidopyrimidine-DNA glycosylase-like protein
- Ribonuclease 3
- Endonuclease III lyase)
- Endonuclease 8 1 lyase Nei 1)
- Protein RecA
- Adenine DNA glycosylase
- DNA polymerase I
- Putative bifunctional exonuclease/endonuclease protein Rv2191
- Conserved protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0043167 (ion binding), GO:0043169 (cation binding), GO:0003676 (nucleic acid binding), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0016798 (hydrolase activity, acting on glycosyl bonds), GO:0140097 (catalytic activity, acting on DNA), GO:0016799 (hydrolase activity, hydrolyzing N-glycosyl compounds), GO:0004518 (nuclease activity), GO:0003677 (DNA binding), GO:0003906 (DNA-(apurinic or apyrimidinic site) endonuclease activity), GO:0019104 (DNA N-glycosylase activity), GO:0046872 (metal ion binding), GO:0003684 (damaged DNA binding), GO:0000702 (oxidized base lesion DNA N-glycosylase activity), GO:0046914 (transition metal ion binding), GO:0004519 (endonuclease activity), GO:0008270 (zinc ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0006807 (nitrogen compound metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0006979 (response to oxidative stress), GO:0046483 (heterocycle metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0034599 (cellular response to oxidative stress), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0062197 (cellular response to chemical stress), GO:0006259 (DNA metabolic process), GO:0006281 (DNA repair), GO:0006289 (nucleotide-excision repair)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)