Protein: CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4 (Q5A761)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Acts as a catalytic component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover (By similarity). Ccr4 has 3'-5' RNase activity with a strong preference for polyadenylated substrates and also low exonuclease activity towards single-stranded DNA (By similarity)
Subcellular location: Cytoplasm. Nucleus
Sequence: MNITNKFQHTPIQGQPQSNLQAQQILLSQLHQGQAQQQQQQQQQQQQQQQSSAGVLGSGFQPSDNFGESLNQNIYQNNYQRAQPSLQQQFFPQFQQSQQQPSQQANNASQLQQPYQPAQQMVRQTSHFQQQQQQQPSSQQFYSQQQASVLQQQQQQQIQLQSAHQQQSSLLNMNSINVDNPNSVYWQHQQQLCQISRGSNVPHYYARQYASNSRKAKNPYSEVKSVGLVEATRSMVASLEDEEEKKKKPINYQGTPTTSAALLHNKKSTQDIFEDDSMEEQRMRLKTQGKQLWCQLDLSGQGLVNISSKLFHYDFLESLYLSNNKLNSIPSSISKLRNLRTLDLSHNRINELPEELGLCFNLRYLFLFDNNIKTLPYSFGNMIELLFIGIEGNPLEPSIANLIAEKGTKELIATLRDQTTVKRTPKPRCWLTLEDDGEVVDSDEVYKVEPESSDNFTVLSYNTLCQHYATPKMYKFTPSWALQWDYRKNLLEKEVLNYNTDIVCMQEVETKTFQEFWLPVMTANGYKGYFFSKTRSKTMSETDSKKVDGCATFFKNDKFSLIHKQNFEYNSVCMGSDKYKKTKDLFNRFMNKDNIALISYLQHKESGEKIAVVNTHLHWDPAFNDVKALQVGILLEELQGIIKKYRHTNSNEDIKNSSIVVCGDFNSVKDSAVYQLFSTGASKGHEDMNGRDYGKFTEDGFHHPFKLKSAYEAVGELPFTNLTPAFTDNIDYIWYSTPTLQVKGLLGGVDEEYTSHSIGFPDANFPSDHVPILAKFQLKKGKKNHSV
InterPro domains: - IPR036691: Endonuclease/exonuclease/phosphatase superfamily (homologous_superfamily) [418-786]
- IPR005135: Endonuclease/exonuclease/phosphatase (domain) [459-769]
- IPR001611: Leucine-rich repeat (repeat) [296-382]
- IPR050410: CCR4/nocturin mRNA turnover and transcription (family) [315-778]
- IPR003591: Leucine-rich repeat, typical subtype (repeat) [316-381]
- IPR032675: Leucine-rich repeat domain superfamily (homologous_superfamily) [230-417]
Interaction partners: - General negative regulator of transcription subunit 1
- CCR4-NOT core DEDD family RNase subunit
- CCR4-NOT core subunit
- CCR4-NOT core ubiquitin-protein ligase subunit
- General negative regulator of transcription subunit
- General negative regulator of transcription subunit 3
- ATP-dependent RNA helicase DHH1
- CCR4-NOT core subunit
- Cdc73p
- RNA polymerase II-associated protein 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0016787 (hydrolase activity), GO:0140098 (catalytic activity, acting on RNA), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0004518 (nuclease activity), GO:0004540 (ribonuclease activity), GO:0004532 (exoribonuclease activity), GO:0004527 (exonuclease activity), GO:0008408 (3'-5' exonuclease activity), GO:0016896 (RNA exonuclease activity, producing 5'-phosphomonoesters), GO:0016796 (exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters), GO:0000175 (3'-5'-RNA exonuclease activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0009892 (negative regulation of metabolic process), GO:0065008 (regulation of biological quality), GO:0044237 (cellular metabolic process), GO:0048522 (positive regulation of cellular process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0048523 (negative regulation of cellular process), GO:0044238 (primary metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0043487 (regulation of RNA stability), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0044248 (cellular catabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009894 (regulation of catabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0009896 (positive regulation of catabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0034655 (nucleobase-containing compound catabolic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0043488 (regulation of mRNA stability), GO:0019439 (aromatic compound catabolic process), GO:0051248 (negative regulation of protein metabolic process), GO:0046700 (heterocycle catabolic process), GO:0050779 (RNA destabilization), GO:0010468 (regulation of gene expression), GO:0009057 (macromolecule catabolic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0034248 (regulation of amide metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0044265 (cellular macromolecule catabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0031331 (positive regulation of cellular catabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0031329 (regulation of cellular catabolic process), GO:0010629 (negative regulation of gene expression), GO:0034249 (negative regulation of amide metabolic process), GO:0017148 (negative regulation of translation), GO:0016070 (RNA metabolic process), GO:0061013 (regulation of mRNA catabolic process), GO:0061014 (positive regulation of mRNA catabolic process), GO:0006402 (mRNA catabolic process), GO:0006401 (RNA catabolic process), GO:2000112 (regulation of cellular macromolecule biosynthetic process), GO:0006417 (regulation of translation), GO:1903311 (regulation of mRNA metabolic process), GO:0061157 (mRNA destabilization), GO:2000113 (negative regulation of cellular macromolecule biosynthetic process), GO:1903313 (positive regulation of mRNA metabolic process), GO:0010608 (post-transcriptional regulation of gene expression), GO:0000956 (nuclear-transcribed mRNA catabolic process), GO:0016071 (mRNA metabolic process), GO:0000288 (nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay), GO:0000289 (nuclear-transcribed mRNA poly(A) tail shortening)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140535 (intracellular protein-containing complex), GO:1902494 (catalytic complex), GO:0140513 (nuclear protein-containing complex), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0099080 (supramolecular complex), GO:0030880 (RNA polymerase complex), GO:0036464 (cytoplasmic ribonucleoprotein granule), GO:1990234 (transferase complex), GO:0043229 (intracellular organelle), GO:0008023 (transcription elongation factor complex), GO:0043228 (non-membrane-bounded organelle), GO:0035770 (ribonucleoprotein granule), GO:0043233 (organelle lumen), GO:0055029 (nuclear DNA-directed RNA polymerase complex), GO:0016591 (RNA polymerase II, holoenzyme), GO:0043227 (membrane-bounded organelle), GO:0000428 (DNA-directed RNA polymerase complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0000932 (P-body), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0061695 (transferase complex, transferring phosphorus-containing groups), GO:0016593 (Cdc73/Paf1 complex), GO:0070013 (intracellular organelle lumen), GO:0005634 (nucleus), GO:0031981 (nuclear lumen)