Protein: Probable vinculin (Ddalpha-catenin) (Q54MH2)
Organism: Dictyostelium discoideum (Social amoeba)
Function: Involved in cell adhesion. Thought to play an important role in cytokinesis B, probably by providing substrate adhesion and traction forces. Required to organize and polarize the tip epithelium during cytokinesis. Required for the normal distribution of myosin in the tip epithelium. Involved in the localization of ctxA, ctxB, dcsA, exoc6 and rgaA. Thought to form a complex with ctxA, ctxB, and rgaA which regulates myosin accumulation to the apical plasma membrane.
Subcellular location: Cytoplasm, cell cortex {ECO:0000269|PubMed:19065153}. Cell junction {ECO:0000269|PubMed:21393547}. Note=Localized during interphase over the cell basal surface with levels decreasing at the basal surface when cells enter the mitotic phase. At this time, there is intense localization along the polar edges (PubMed:19065153). Also found at cell-cell contacts in the slug and fruiting body and in columnar cells of the tip epithelium (PubMed:21393547). {ECO:0000269|PubMed:19065153, ECO:0000269|PubMed:21393547}.
Sequence: MDEVLEMIADAVSSLVVAITDSEEKNTLFGDMVPGVELIQQAVNGMAEAAEETVSLIDEEFIGQLESTSKQLKNSAGQLYVHAVRAREDPWNRVPQKDAIKAAKQILQNVVLLVLIEEQSNIKVLVNIAKKAAEGVRRIDEIENIKQLDVMIGDVNQLQNELVKRSQRRSEGSHNPELRSKLEDIATMVNILSEQHQASARDVCRNPREETLRSKRSELSSKLLSAIDDLIYTIKLIFENNTKFVDLAFKWKPVRTMAEDEVTRASAVLIDNLRTLPKSIEAGNGPAAAREIVNAANLQISNAIIVANRCQDPVKKKMLLKQIEELKKLTPMLISAMKPVLENPNDQEAQKHLESVIYSTQKASEALATAVVSSPAEIVAASGVSLARDLDSLEEAIASGDKKRAQVILSHIPSAIDKHIELANALLETITDPGQRHQIKQSIERLQTLKPRIIENANRAIANPNDHEARKNLSSDIKEAKKAIGQISQPYEVVSALNTKIHNDLDSLIKCIDEGGPDMQVKGVQYAKDIANSIKKQIEAAEAYAQTITDPDRKKQVLDSIEQLKKLTPQLLEAIRACLANPDDKEARKRLDDVVRRVKEASSNLSQVIQPTADELKEEKRKRNEEIARIEAEEKAKARALLKAAELARIEAEEEKKRLAIIEEEKKRLAAEEEERKRAPKLVVPEGPVNKAVFGAAADVAQALESKVRDGTPLGILVQLSDEIAQQMALIASFAMNGDVKGMITAARKIADTIKQVQTQAKHIADNCTDPRLKQNVLTYCDCGGNFSTQLKILCAVKSNDFNDPTAEEQLVTCAKGLSGAVINLVKSSEAASIKQRKVPQQ
InterPro domains: - IPR017997: Vinculin (family) [250-838]
- IPR006077: Vinculin/alpha-catenin (family) [3-838]
- IPR054082: Talin, IBS2B domain (domain) [520-609]
- IPR036723: Alpha-catenin/vinculin-like superfamily (homologous_superfamily) [32-840]
Interaction partners: - Talin-B
- LIM domain-containing protein DDB_G0271356
- Paxillin-B
- Protein VASP homolog
- Cofilin-1A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0044877 (protein-containing complex binding), GO:0005515 (protein binding), GO:0051015 (actin filament binding), GO:0008092 (cytoskeletal protein binding), GO:0003779 (actin binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0042221 (response to chemical), GO:1901698 (response to nitrogen compound), GO:0010033 (response to organic substance), GO:0010243 (response to organonitrogen compound), GO:0043279 (response to alkaloid)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0043229 (intracellular organelle), GO:0005938 (cell cortex), GO:0043228 (non-membrane-bounded organelle), GO:0030863 (cortical cytoskeleton), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0030864 (cortical actin cytoskeleton), GO:0015629 (actin cytoskeleton)