Protein: Homeobox protein notochord (Q5TIS6)
Organism: Mus musculus (Mouse)
Function: Transcription factor that controls node morphogenesis. Acts downstream of both FOXA2 and Brachyury (T) during notochord development. Is essential for cilia formation in the posterior notochord (PNC) and for left-right patterning; acts upstream of FOXJ1 and RFX3 in this process and is required for the expression of various components important for axonemal assembly and function. Plays a role in regulating axial versus paraxial cell fate. Activates the transcription of ciliary proteins C11orf97 homolog, FAM183B and SPACA9 in the embryonic ventral node
Subcellular location: Nucleus
Sequence: MSSPAPSGTQVQPGSLRPCPGAVSPVVPRRLARGRLESSFSVEAILARPKTRELAATSLPLSTCTSLNLLGAVSQYGVLPWVCSTGSWLPAYLSVGVYPLCSMSCVPGLNVTHHQQGLRLTGSELPYCLGPLKWAPTVDLRDHGTERHTKRVRTTFNLQQLQELEKVFAKQHNLVGKERAQLAARLHLTENQVRIWFQNRRVKYQKQQKLKLPSSSVMEEPSSSSDGNIQSEDAELGIGS
InterPro domains: - IPR001356: Homeodomain (domain) [147-211]
- IPR009057: Homedomain-like superfamily (homologous_superfamily) [139-209]
- IPR050877: EMX/VAX/Noto Homeobox Transcription Factors (family) [12-227]
Interaction partners: - Hepatocyte nuclear factor 3-beta
- T-box transcription factor T
- Hepatocyte nuclear factor 3-alpha
- Transcriptional enhancer factor TEF-4
- Transcriptional coactivator YAP1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:1901363 (heterocyclic compound binding), GO:0003700 (DNA-binding transcription factor activity), GO:0097159 (organic cyclic compound binding), GO:0003676 (nucleic acid binding), GO:0001217 (DNA-binding transcription repressor activity), GO:0000981 (DNA-binding transcription factor activity, RNA polymerase II-specific), GO:0001227 (DNA-binding transcription repressor activity, RNA polymerase II-specific), GO:0001067 (transcription regulatory region nucleic acid binding), GO:0003677 (DNA binding), GO:0003690 (double-stranded DNA binding), GO:0043565 (sequence-specific DNA binding), GO:0000976 (transcription cis-regulatory region binding), GO:1990837 (sequence-specific double-stranded DNA binding), GO:0000987 (cis-regulatory region sequence-specific DNA binding), GO:0000977 (RNA polymerase II transcription regulatory region sequence-specific DNA binding), GO:0000978 (RNA polymerase II cis-regulatory region sequence-specific DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0007389 (pattern specification process), GO:0050794 (regulation of cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0035295 (tube development), GO:0009887 (animal organ morphogenesis), GO:0048598 (embryonic morphogenesis), GO:0048729 (tissue morphogenesis), GO:0044085 (cellular component biogenesis), GO:0009888 (tissue development), GO:0003002 (regionalization), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0044087 (regulation of cellular component biogenesis), GO:0009799 (specification of symmetry), GO:0009790 (embryo development), GO:0016043 (cellular component organization), GO:0051128 (regulation of cellular component organization), GO:0009880 (embryonic pattern specification), GO:0035239 (tube morphogenesis), GO:0009855 (determination of bilateral symmetry), GO:0060562 (epithelial tube morphogenesis), GO:0048568 (embryonic organ development), GO:0030030 (cell projection organization), GO:0003007 (heart morphogenesis), GO:0031344 (regulation of cell projection organization), GO:0060972 (left/right pattern formation), GO:0035050 (embryonic heart tube development), GO:0048562 (embryonic organ morphogenesis), GO:0072359 (circulatory system development), GO:0033043 (regulation of organelle organization), GO:0022607 (cellular component assembly), GO:0003143 (embryonic heart tube morphogenesis), GO:0009953 (dorsal/ventral pattern formation), GO:0002009 (morphogenesis of an epithelium), GO:0060429 (epithelium development), GO:0006996 (organelle organization), GO:1902115 (regulation of organelle assembly), GO:0060491 (regulation of cell projection assembly), GO:0007507 (heart development), GO:0070925 (organelle assembly), GO:0061371 (determination of heart left/right asymmetry), GO:0007368 (determination of left/right symmetry), GO:0120032 (regulation of plasma membrane bounded cell projection assembly), GO:0030903 (notochord development), GO:0120035 (regulation of plasma membrane bounded cell projection organization), GO:0030031 (cell projection assembly), GO:1902017 (regulation of cilium assembly), GO:0120036 (plasma membrane bounded cell projection organization), GO:0044782 (cilium organization), GO:0001947 (heart looping), GO:0120031 (plasma membrane bounded cell projection assembly), GO:0060271 (cilium assembly), GO:0044458 (motile cilium assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)