Protein: Homocitrate synthase, mitochondrial (HCS) (EC 2.3.3.14) (Q12122)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Catalyzes the aldol-type condensation of 2-oxoglutarate with acetyl-CoA to yield homocitrate. Carries out the first step of the alpha-aminoadipate (AAA) lysine biosynthesis pathway.
Subcellular location: Mitochondrion {ECO:0000305}.
Sequence: MSENNEFQSVTESTTAPTTSNPYGPNPADYLSNVKNFQLIDSTLREGEQFANAFFDTEKKIEIARALDDFGVDYIELTSPVASEQSRKDCEAICKLGLKAKILTHIRCHMDDARVAVETGVDGVDVVIGTSKFLRQYSHGKDMNYIAKSAVEVIEFVKSKGIEIRFSSEDSFRSDLVDLLNIYKTVDKIGVNRVGIADTVGCANPRQVYELIRTLKSVVSCDIECHFHNDTGCAIANAYTALEGGARLIDVSVLGIGERNGITPLGGLMARMIVAAPDYVRSKYKLHKIRDIENLVADAVEVNIPFNNPITGFCAFTHKAGIHAKAILANPSTYEILDPHDFGMKRYIHFANRLTGWNAIKSRVDQLNLNLTDDQIKEVTAKIKKLGDVRPLNIDDVDSIIKDFHAELSTPLLKPVNKGTDDDNIDISNGHVSKKAKVTK
InterPro domains: - IPR048253: Homocitrate synthase, catalytic TIM barrel domain, fungi/bacteria (domain) [37-298]
- IPR002034: Alpha-isopropylmalate/homocitrate synthase, conserved site (conserved_site) [44-236]
- IPR054691: 2-isopropylmalate synthase/homocitrate synthase, post-catalytic domain (domain) [310-390]
- IPR013785: Aldolase-type TIM barrel (homologous_superfamily) [26-302]
- IPR011872: Homocitrate synthase (family) [38-394]
- IPR000891: Pyruvate carboxyltransferase (domain) [37-296]
- IPR050073: 2-IPM synthase/homocitrate synthase-like (family) [32-424]
Interaction partners: - Homocitrate synthase, cytosolic isozyme
- Homocitrate dehydratase, mitochondrial
- Homoisocitrate dehydrogenase, mitochondrial
- 3-isopropylmalate dehydratase
- Acetyl-coenzyme A synthetase 2
- 2-isopropylmalate synthase
- Acetyl-coenzyme A synthetase 1
- Acetyl-CoA acetyltransferase
- 2-isopropylmalate synthase 2, mitochondrial
- Acetyl-CoA hydrolase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016746 (acyltransferase activity), GO:0046912 (acyltransferase activity, acyl groups converted into alkyl on transfer)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0006082 (organic acid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0006259 (DNA metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0009066 (aspartate family amino acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0006281 (DNA repair), GO:0006553 (lysine metabolic process), GO:0009067 (aspartate family amino acid biosynthetic process), GO:0009085 (lysine biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)