Protein: E3 ubiquitin-protein ligase RNF149 (EC 2.3.2.27) (Goliath-related E3 ubiquitin-protein ligase 4) (RING finger protein 149) (RING-type E3 ubiquitin transferase RNF149) (Q3U2C5)
Organism: Mus musculus (Mouse)
Function: E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation.
Subcellular location: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
Sequence: MAARRRPAAGVGARDALAVLALALCTPGVGGGALEWYSAMVSIEYVDPQSNLTVWSVSESGRFGESSLREERQGLVGVPRAPAPAEGCAPDTRFVAPGALGNAPWVALVARGGCTFKDKVLAAARRNASAVVVYNLESNGNATEPMSHAGTGNIVVIMISYPKGREIFDLVQKGIPVKMRIEIGTRHMQEFISGQSVVFVAIAFITMMIISLAWLIFYYIQRFLYTGSQFGSQNHRKETKKVIGQLPLHTVKHGEKGIDVDAENCAVCIENFKVKDVIRILPCKHIFHRICIDPWLLDHRTCPMCKLDVIKALGYWGDPEDTQELPTPEAAPGRVSVGNLSVTSQDEERSESNLPSSSSSESGPHRPCLKEDAGEDTALLGAGRSEPQHGGSIC
InterPro domains: - IPR046450: PA domain superfamily (homologous_superfamily) [87-184]
- IPR042712: E3 ubiquitin-protein ligase RNF149, RING finger, H2 subclass (domain) [264-311]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [226-311]
- IPR003137: PA domain (domain) [84-167]
- IPR001841: Zinc finger, RING-type (domain) [264-306]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0019787 (ubiquitin-like protein transferase activity), GO:0004842 (ubiquitin-protein transferase activity), GO:0061659 (ubiquitin-like protein ligase activity), GO:0061630 (ubiquitin protein ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0065007 (biological regulation), GO:0019222 (regulation of metabolic process), GO:0051241 (negative regulation of multicellular organismal process), GO:0051239 (regulation of multicellular organismal process), GO:0009892 (negative regulation of metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0001818 (negative regulation of cytokine production), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0001817 (regulation of cytokine production), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0005773 (vacuole), GO:0043227 (membrane-bounded organelle), GO:0000323 (lytic vacuole), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0012506 (vesicle membrane), GO:0005770 (late endosome), GO:0005774 (vacuolar membrane), GO:0010008 (endosome membrane), GO:0031982 (vesicle), GO:0030659 (cytoplasmic vesicle membrane), GO:0005764 (lysosome), GO:0097708 (intracellular vesicle), GO:0031902 (late endosome membrane), GO:0098852 (lytic vacuole membrane), GO:0005765 (lysosomal membrane)