Protein: Tyrosine-protein phosphatase CDC14 (Q59NH8)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Protein phosphatase which antagonizes mitotic cyclin- dependent kinase CDC28, the inactivation of which is essential for exit from mitosis. To access its substrates, is released from nucleolar sequestration during mitosis. Plays an essential in coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases (By similarity). Plays a role in the expression of hydrolase genes such as CHT3 and ENG1 involved in septum degradation after cytokinesis. Also required for ACE2 localization to the daughter nucleus. Required for invasive and hyphal growth
Subcellular location: Nucleus, nucleolus. Cytoplasm. Bud neck. Cytoplasm, cytoskeleton, spindle pole. Cell septum. Note=Does not accumulate in the nucleolus in interphase cells. At the G1-S transition, starts to accumulate both in the nucleolus and the and the DAPI-staining region of the nucleus. At the S-G2 transition, starts to concentrate as faint foci on the nuclear periphery. In early mitosis, the CDC14 nuclear signal diminishes concentrates to the spindle pole body (SPB). During cytokinesis, the localization at the SPBs gets fainter and the protein is preferentially found at the septum region. In yeast forms, in which cell separation takes place after cytokinesis, CDC14 is present at the bud neck; in hyphal forms, in which cell separation is inhibited, no CDC14 signal is detected
Sequence: MHSSSVHVPLIEFLKNRIYLGAYDHHKRDTEDLAYFTVEDALPYNAFYMDFGPLNIGHLYRFAVLLHKKLNEDSTQGKGLVIYSSTSPKERANLACLLCCYMILLQNWAPHQVLQPIAQITPPLQAFRDAGYSSADYEITIQDVVYAMWRAKERGMIDLAKFDLDEYEQYERVDQGDFNVISKDFIAFASPQQSKRGGLNEPFQKVLEYFVENNVQLVVRLNSHLYDAKEFTKRNIKHIDMIFDDGTCPTLEYVQKFIGAAECIINKGGKIAVHCKAGLGRTGCLIGAHLIYTHGFTANECIAYMRMIRPGMVVGPQQHWLYLHHDDFRSWRHTMIVDNRPDPLIGNLFPLCSYEDYKQRLKEAKRKERLQLQQQLTSPLADSSVINTPIRRRKVSGALASKIQTVVPIESPGQPRKYFEDSEDIDEVEMVNNSDDENTMQDIIQSSPARYDSVTPKTKDNSDWRVLRSISTNNVSSQQSIHIIKTTTTKTVNETLSSPPGTSPTNVLRVSKARSKNRIASGNSQTSRAHSGGVRKLSGKKH
InterPro domains: - IPR044506: Dual-specificity phosphatase CDC14, C-terminal (domain) [161-331]
- IPR003595: Protein-tyrosine phosphatase, catalytic (domain) [234-328]
- IPR016130: Protein-tyrosine phosphatase, active site (active_site) [273-283]
- IPR000387: Tyrosine-specific protein phosphatases domain (domain) [255-320]
- IPR029021: Protein-tyrosine phosphatase-like (homologous_superfamily) [3-336]
- IPR050561: Protein Tyrosine Phosphatase (family) [36-502]
- IPR000340: Dual specificity phosphatase, catalytic domain (domain) [201-325]
- IPR020422: Dual specificity protein phosphatase domain (domain) [177-334]
- IPR029260: Dual specificity/tyrosine protein phosphatase, N-terminal (domain) [10-152]
Interaction partners: - Cell cycle protein kinase DBF2
- Serine/threonine protein kinase
- Serine/threonine-protein kinase
- Ras family GTPase
- Oxidant-induced cell-cycle arrest protein 5
- separase
- Mob1p
- Mitosis inhibitor protein kinase SWE1
- Cdh1p
- Serine/threonine-protein kinase CBK1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0004721 (phosphoprotein phosphatase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0042578 (phosphoric ester hydrolase activity), GO:0004725 (protein tyrosine phosphatase activity), GO:0016791 (phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0040007 (growth), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0009605 (response to external stimulus), GO:0051716 (cellular response to stimulus), GO:0030447 (filamentous growth), GO:0006950 (response to stress), GO:0009607 (response to biotic stimulus), GO:0007154 (cell communication), GO:0009991 (response to extracellular stimulus), GO:0031668 (cellular response to extracellular stimulus), GO:0044182 (filamentous growth of a population of unicellular organisms), GO:0036180 (filamentous growth of a population of unicellular organisms in response to biotic stimulus), GO:0042594 (response to starvation), GO:0071496 (cellular response to external stimulus), GO:0033554 (cellular response to stress), GO:0036170 (filamentous growth of a population of unicellular organisms in response to starvation), GO:0009267 (cellular response to starvation), GO:0031669 (cellular response to nutrient levels), GO:0031667 (response to nutrient levels)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005815 (microtubule organizing center), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0099080 (supramolecular complex), GO:0099081 (supramolecular polymer), GO:0005816 (spindle pole body), GO:0043229 (intracellular organelle), GO:0005938 (cell cortex), GO:0043228 (non-membrane-bounded organelle), GO:0030863 (cortical cytoskeleton), GO:0099512 (supramolecular fiber), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0099513 (polymeric cytoskeletal fiber), GO:0030864 (cortical actin cytoskeleton), GO:0005874 (microtubule), GO:0015630 (microtubule cytoskeleton)