Protein: Inorganic pyrophosphatase TTM1 (EC 3.6.1.1) (Triphosphate tunnel metalloenzyme 1) (AtTTM1) (Q9C9B9)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Exhibits pyrophosphatase activity with stronger affinity for pyrophosphate (PPi), moderate affinity for ATP and ADP, and weak affinity for tripolyphosphate (PPPi). No activity observed toward uridine substrate. Regulates positively natural and dark-induced leaf senescence.
Subcellular location: Mitochondrion outer membrane {ECO:0000269|PubMed:28733390}; Single-pass membrane protein {ECO:0000255}.
Sequence: MALDSSVALSPRRRHGLLRDQVQLIKRKDSGRYEIVPIEDPLSFEKGFYAVIRACQLLAQKNDGLILVGLAGPSGAGKTIFTEKILNFMPSIAIINMDNYNDGTRVIDGNFDDPRLTDYDTLLDNIHGLRDGKPVQVPIYDFKSSSRIGYRTLEVPSSRIVILEGIYALSEKLRPLLDLRVSVTGGVHFDLVKRVLRDIQRAGQEPEEIIHQISETVYPMYKAFIEPDLKTAQIKILNKFNPFSGFQNPTYILKSSKAVTPEQMKAALSEDFKERTEETYDIYLLPPGEDPEACQSYLRMRNRDGKYNLMFEEWVTDRPFIISPRITFEVSVRLLGGLMALGYTIATILKRKSHIFDDDKVIVKTDWLEQLNRTYVQVQGKDRTFVKNVADQLGLEGSYVPHTYIEQIQLERLVNDVLALPDDLKTKLSLDDDTVSSPKEALSRASVDSRMKYLHGGVSKSYTNPRHKVLPNLTRLAVNNRMLDARAPASPATLPNQGFITQLSDQISTLNERMDEFTSRIEELNSKIPNRIAPSGSQHNLALPIENGNGSVLSFSASASQLVRESPLMEEVVLVARGQRQIMHQMDTLSNLLREYVGEKTRIERLDSSRTNSTTQNLESSTVPILLGLAIGCVGIFAYSRLK
InterPro domains: - IPR006083: Phosphoribulokinase/uridine kinase (domain) [68-236]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [63-244]
- IPR033469: CYTH-like domain superfamily (homologous_superfamily) [270-407]
- IPR023577: CYTH domain (domain) [248-410]
Interaction partners: - Uridine nucleosidase 1
- Uridine 5'-monophosphate synthase
- Uracil phosphoribosyltransferase, chloroplastic
- UMP kinase
- dCMP deaminase
- Alkaline-phosphatase-like family protein
- Dihydroorotate dehydrogenase
- Cytidine deaminase 5
- Cytidine deaminase 6
- Cytidine deaminase 7
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0016462 (pyrophosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0042221 (response to chemical), GO:0009607 (response to biotic stimulus), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0023051 (regulation of signaling), GO:0006950 (response to stress), GO:0051707 (response to other organism), GO:0048585 (negative regulation of response to stimulus), GO:0002831 (regulation of response to biotic stimulus), GO:0043207 (response to external biotic stimulus), GO:0010646 (regulation of cell communication), GO:0080134 (regulation of response to stress), GO:0009617 (response to bacterium), GO:0009966 (regulation of signal transduction), GO:0006952 (defense response), GO:0032101 (regulation of response to external stimulus), GO:1901700 (response to oxygen-containing compound), GO:0002832 (negative regulation of response to biotic stimulus), GO:0010033 (response to organic substance), GO:0002239 (response to oomycetes), GO:0098542 (defense response to other organism), GO:0031348 (negative regulation of defense response), GO:0032102 (negative regulation of response to external stimulus), GO:0031347 (regulation of defense response), GO:2000031 (regulation of salicylic acid mediated signaling pathway), GO:0009751 (response to salicylic acid), GO:0010112 (regulation of systemic acquired resistance), GO:1900425 (negative regulation of defense response to bacterium), GO:0002237 (response to molecule of bacterial origin), GO:0014070 (response to organic cyclic compound), GO:1900424 (regulation of defense response to bacterium)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0031090 (organelle membrane), GO:0031967 (organelle envelope), GO:0005739 (mitochondrion), GO:0043229 (intracellular organelle), GO:0019867 (outer membrane), GO:0043227 (membrane-bounded organelle), GO:0005740 (mitochondrial envelope), GO:0031968 (organelle outer membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0031966 (mitochondrial membrane), GO:0005741 (mitochondrial outer membrane)