Protein: Eukaryotic translation initiation factor 5B (eIF-5B) (EC 3.6.5.3) (Translation initiation factor IF-2) (Q05D44)
Organism: Mus musculus (Mouse)
Function: Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:12426392}.
Sequence: MGKKQKNKSEDSTKDDTDLGALAAEIEGAGAAKEQEPQKSKGKKKKEKKKQDFDENDILRELEELSLEAQGIRADRDAAAVKPTENNEEESASKQDKKKKGQKGKKTSFDENDSEELEDKDSKSKKTARPNSEAPLSGSEDADDSNKLSKKGKKAQKSTKKRDGSEEDEDNSKRSKERSRVNSSGESGGESDEFLQSRKGQKKNQKNKSVPTVDSGNEDDDSSFKIKTVAQKKAEKKEREKKKRDEEKAKLRKMKEKEELEKGKKEQSKQREPQKRPEEEVLTLRGTPDTGAASEEKGDTAAALEDDNEGDKKKKDKKKKKTEKDEKEKEKKKGPSKSTVKAIQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEVTLRHLQAQGVEVPSKDSLPKKRPVYEDKKKKKTPQQLESKEVSETLEISAPVEAVDQGGPEKEETPPSVEPEEEEDTEDAGLDDWEAMASDEEREKEGNMIHIEVEENPEEEEEEEEEEEEEESEDEEEEGDSEGSDGDEEDCKLSDEKDSGKAGDTKPSKDASSDSEYDSDDDRTKEERAYDKAKRRIEKRRLEHGKNVNTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINILKSKKCPFIVALNKIDRLYDWKKSPDSDVAVTLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKDDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKMKILPQYIFNSRDPIVIGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII
InterPro domains: - IPR036925: Translation initiation factor IF-2, domain 3 superfamily (homologous_superfamily) [960-1080]
- IPR000795: Translational (tr)-type GTP-binding domain (domain) [625-842]
- IPR015760: Translation initiation factor IF- 2 (family) [303-1209]
- IPR005225: Small GTP-binding domain (domain) [630-768]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [619-916]
- IPR009000: Translation protein, beta-barrel domain superfamily (homologous_superfamily) [1084-1214]
- IPR023115: Translation initiation factor IF- 2, domain 3 (domain) [961-1065]
- IPR029459: Elongation factor Tu-type domain (domain) [1087-1178]
Interaction partners: - Small ribosomal subunit protein uS3
- Small ribosomal subunit protein uS19
- Eukaryotic translation initiation factor 5
- Ubiquitin-like protein FUBI
- Small ribosomal subunit protein uS5
- Small ribosomal subunit protein uS4
- Large ribosomal subunit protein uL18
- Large ribosomal subunit protein uL23
- Small ribosomal subunit protein uS17
- ATPase inhibitor, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044238 (primary metabolic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0006413 (translational initiation), GO:0019538 (protein metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006412 (translation), GO:0009059 (macromolecule biosynthetic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0006518 (peptide metabolic process), GO:0010467 (gene expression), GO:0043043 (peptide biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0005737 (cytoplasm), GO:0030054 (cell junction), GO:0045202 (synapse)