Protein: Smu-2 suppressor of mec-8 and unc-52 protein (RED homolog) (Suppressor of Mec and Unc defects 2) (Q9N4U5)
Organism: Caenorhabditis elegans
Function: Auxiliary spliceosomal protein that regulates selection of alternative splice sites in a small set of target pre-mRNA species (Probable). Selectively regulates alternative splicing of unc-52 exon 17. Thus, smu-2 mutants selectively suppress the effects of unc-52 nonsense mutations in exon 17 by promoting the accumulation of unc-52 isoforms that lack exon 17. In contrast, smu-2 mutants do not suppress the effects of an unc-52 mutation that affects the 5' splice site of exon 16. Required for normal accumulation of smu-1.
Subcellular location: Nucleus {ECO:0000269|PubMed:15254247}.
Sequence: MADNPTNLRNADFRKLLTSARSDRPAVSAFAKPADPKTGDDKPASFKHKHLKPAKFKKPQAAAHGKAKKEKTEADEDEAALKNILKNYRDRAAERRKQGDEKEDPSKLTAAYRAVPGDARSAQDQADLRKQAILESKYLGGDLEHTHLVKGLDYSLLNKVRSEIDKSDDDDDDDIDTAFDEKVTSSSSSSKPSEASLLAQELAQSHSENRMVRSLHRVLFKNEVPLHNQLFAKGRMAYVVELEDEETDIPTTLLRSLHDLPRAESAQSIQANNLIILKLSHVLSHLRAEPKKKKKEEFRVQLGSRDAPGAAAAAPGAKGDSIYDDLDDYVPSRKSRDSRDAGRRGSRRDRSRDRSRDRDRDRDRDNRDRYFEKSANSRREEEQNRREQQRERERAEQERRREREKEREQEKAKEREKKRKELEESSGYDECYPGGLVEMGGAWDSDEEADYSKMDAGPKKNQAVNRWDFDTEEEYASYMEGREALPKAAYQYGVKNGEGGRKNKKQSAVSDAKRLDRELNEINKIMDKRKAGGDGAGGGGDYKKPKY
InterPro domains: - IPR012492: Protein RED, C-terminal (domain) [428-537]
- IPR039896: Protein Red-like (family) [9-546]
- IPR012916: RED-like, N-terminal (domain) [66-295]
Interaction partners: - Smu-1 suppressor of mec-8 and unc-52 protein
- Pre-mRNA-processing factor 6
- Microfibrillar-associated protein 1
- Tr-type G domain-containing protein
- Mec-8 protein
- Splicing factor 3B subunit 3
- Pre-mRNA-splicing factor 38
- Peptidase M14 carboxypeptidase A domain-containing protein
- F-box associated domain-containing protein
- Basement membrane proteoglycan
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0007267 (cell-cell signaling), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0007154 (cell communication), GO:0044238 (primary metabolic process), GO:0099536 (synaptic signaling), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0099537 (trans-synaptic signaling), GO:0099172 (presynapse organization), GO:0034330 (cell junction organization), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0098916 (anterograde trans-synaptic signaling), GO:0072553 (terminal button organization), GO:0050808 (synapse organization), GO:0006396 (RNA processing), GO:0008380 (RNA splicing), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0007528 (neuromuscular junction development), GO:0007268 (chemical synaptic transmission), GO:0000398 (mRNA splicing, via spliceosome), GO:0000375 (RNA splicing, via transesterification reactions), GO:0007274 (neuromuscular synaptic transmission), GO:0000377 (RNA splicing, via transesterification reactions with bulged adenosine as nucleophile)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)