Protein: Sprouty-related, EVH1 domain-containing protein 1 (Spred-1) (Q924S8)
Organism: Mus musculus (Mouse)
Function: Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow. Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2. Attenuates actin stress fiber formation via inhibition of TESK1-mediated phosphorylation of cofilin (By similarity). Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells.
Subcellular location: Cell membrane {ECO:0000269|PubMed:11493923}; Peripheral membrane protein {ECO:0000269|PubMed:11493923}. Membrane, caveola {ECO:0000269|PubMed:11493923}; Peripheral membrane protein {ECO:0000269|PubMed:11493923}. Nucleus {ECO:0000269|PubMed:11493923}. Note=Localized in cholesterol-rich membrane raft/caveola fractions. {ECO:0000250}.
Sequence: MSEETATSDNDNSYARVRAVVMTRDDSSGGWLPLGGSGLSSVTVFRVPHQEENGCADFFIRGERLRDKMVVLECMLKKDLIYNKVTPTFHHWKIDDKKFGLTFQSPADARAFDRGIRRAIEDISLGCPASKTEAEGGDDDLQTTEEDTSRSLVKDHFFQQETVVTSEPYRSSDIRPLPFEDLNARRVYLQSQVSQIPFSQQGLDIQSRSMEYVQRQISKECGSLKSQTRVPLKSIRHVSFQDEDEIVRINPRDILIRRYADYRHPDMWKNDLERDDTDSSVPFSKQDSKKSDYLYHCGDETKLSSLKDSVVFKTQPPSLKFKSKRRKEDGERSRCVYCQERFNHEENARGKCQDAPDPVKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRWLALVALSFIVPCMCCYVPLRMCHRCGEACGCCGGKHKAAG
InterPro domains: - IPR000697: WH1/EVH1 domain (domain) [6-123]
- IPR041937: SPRE, EVH1 domain (domain) [13-125]
- IPR007875: Sprouty (family) [332-442]
- IPR023337: c-Kit-binding domain (domain) [234-286]
- IPR011993: PH-like domain superfamily (homologous_superfamily) [3-131]
Interaction partners: - Neurofibromin
- GTPase HRas
- GTPase KRas
- GTPase NRas
- Sprouty-related, EVH1 domain-containing protein 2
- Phosphatidylinositol 3-kinase regulatory subunit beta
- GTP-binding protein REM 1
- Proteasome subunit beta type-6
- Fibroblast growth factor receptor-like 1
- Ras GTPase-activating protein 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0048519 (negative regulation of biological process), GO:0023056 (positive regulation of signaling), GO:0023057 (negative regulation of signaling), GO:0048584 (positive regulation of response to stimulus), GO:0019222 (regulation of metabolic process), GO:0051241 (negative regulation of multicellular organismal process), GO:0051239 (regulation of multicellular organismal process), GO:0009892 (negative regulation of metabolic process), GO:0050793 (regulation of developmental process), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0023051 (regulation of signaling), GO:0051093 (negative regulation of developmental process), GO:0048523 (negative regulation of cellular process), GO:0048585 (negative regulation of response to stimulus), GO:0048522 (positive regulation of cellular process), GO:0090287 (regulation of cellular response to growth factor stimulus), GO:0009967 (positive regulation of signal transduction), GO:0009968 (negative regulation of signal transduction), GO:0010648 (negative regulation of cell communication), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0080134 (regulation of response to stress), GO:0080135 (regulation of cellular response to stress), GO:0009966 (regulation of signal transduction), GO:0010719 (negative regulation of epithelial to mesenchymal transition), GO:0060255 (regulation of macromolecule metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:2001022 (positive regulation of response to DNA damage stimulus), GO:2000026 (regulation of multicellular organismal development), GO:0010646 (regulation of cell communication), GO:0045596 (negative regulation of cell differentiation), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0045595 (regulation of cell differentiation), GO:0010647 (positive regulation of cell communication), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0090101 (negative regulation of transmembrane receptor protein serine/threonine kinase signaling pathway), GO:0051246 (regulation of protein metabolic process), GO:0051248 (negative regulation of protein metabolic process), GO:0090092 (regulation of transmembrane receptor protein serine/threonine kinase signaling pathway), GO:1902532 (negative regulation of intracellular signal transduction), GO:2001020 (regulation of response to DNA damage stimulus), GO:0030857 (negative regulation of epithelial cell differentiation), GO:0010563 (negative regulation of phosphorus metabolic process), GO:1902533 (positive regulation of intracellular signal transduction), GO:1903844 (regulation of cellular response to transforming growth factor beta stimulus), GO:0010717 (regulation of epithelial to mesenchymal transition), GO:1902531 (regulation of intracellular signal transduction), GO:0051174 (regulation of phosphorus metabolic process), GO:0030856 (regulation of epithelial cell differentiation), GO:0043408 (regulation of MAPK cascade), GO:1901796 (regulation of signal transduction by p53 class mediator), GO:0031399 (regulation of protein modification process), GO:0031400 (negative regulation of protein modification process), GO:0017015 (regulation of transforming growth factor beta receptor signaling pathway), GO:0019220 (regulation of phosphate metabolic process), GO:0030512 (negative regulation of transforming growth factor beta receptor signaling pathway), GO:1901798 (positive regulation of signal transduction by p53 class mediator), GO:0043516 (regulation of DNA damage response, signal transduction by p53 class mediator), GO:0045936 (negative regulation of phosphate metabolic process), GO:0043409 (negative regulation of MAPK cascade), GO:0042325 (regulation of phosphorylation), GO:0001933 (negative regulation of protein phosphorylation), GO:0001932 (regulation of protein phosphorylation), GO:0042326 (negative regulation of phosphorylation), GO:0070373 (negative regulation of ERK1 and ERK2 cascade), GO:0090311 (regulation of protein deacetylation), GO:0070372 (regulation of ERK1 and ERK2 cascade), GO:0010801 (negative regulation of peptidyl-threonine phosphorylation), GO:0010799 (regulation of peptidyl-threonine phosphorylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0048471 (perinuclear region of cytoplasm), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0097708 (intracellular vesicle)