Protein: Heat shock factor protein 4 (Q5CZP2)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Heat-shock transcription factor that specifically binds heat shock promoter elements (HSE). Required for denucleation and organelle rupture and degradation that occur during eye lens terminal differentiation, when fiber cells that compose the lens degrade all membrane-bound organelles in order to provide lens with transparency to allow the passage of light. In this process, may regulate denucleation of lens fiber cells in part by activating dnase1l1l and dnase2b transcription. May be involved in DNA repair through the transcriptional regulation of rad51. May up-regulate TP53 protein in lens fiber cells, possibly through protein stabilization. In the eye lens, controls the expression of alpha-crystallin B chain/CRYAB and consequently may be involved in the regulation of lysosomal acidification.
Subcellular location: Nucleus {ECO:0000250|UniProtKB:Q9ULV5}.
Sequence: MQENPGSIGVDGGYASNVPAFLTKLWTLVEDPETNHLICWSATGTSFHVFDQGRFAKEVLPKYFKHNNMASFVRQLNMYGFRKVVNIEQSGLVKPERDDTEFQHLYFLQGHEHLLEHIKRKVSIVKSEETKVRQEDLSKLLYEVQVLRSQQENMEMQMQDMKQQNDVLWREVVSLRQNHTQQQKVMNKLIQFLFSQMQSNSPSTVGMKRKLPLMLDDGCSTPPASKFSHNHSMESLQESFYIQSPSTESASCSTSSVMTGGPIISDVTEIPQSSSMALQMQAEESREKCLMLIKEEPVSPGVQGRAEGVPLGSCEVCAEPPVLPVAMVQSVLEGRGSNLGERRAKRPMLERPEIPDGVENVDMSLEDLQLLLRSHQQSMENNAAAMDQFTFSLPLNEWNFAEMDPNLKSELANALIPAAVSQYMFQGQEGELYPTAGYEEQ
InterPro domains: - IPR000232: Heat shock factor (HSF)-type, DNA-binding (domain) [17-121]
- IPR036390: Winged helix DNA-binding domain superfamily (homologous_superfamily) [16-121]
- IPR010542: Vertebrate heat shock transcription factor, C-terminal domain (domain) [249-409]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [13-123]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0042802 (identical protein binding), GO:0003677 (DNA binding), GO:0043565 (sequence-specific DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0050896 (response to stimulus), GO:0032501 (multicellular organismal process), GO:0009628 (response to abiotic stimulus), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0006950 (response to stress), GO:0009408 (response to heat), GO:0009266 (response to temperature stimulus), GO:0002088 (lens development in camera-type eye), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0048880 (sensory system development), GO:0007423 (sensory organ development), GO:0001654 (eye development), GO:0150063 (visual system development), GO:0043010 (camera-type eye development)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000785 (chromatin), GO:0005737 (cytoplasm), GO:0000792 (heterochromatin), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome), GO:0005634 (nucleus)