Protein: Ephrin-B2 (ELF-2) (EPH-related receptor tyrosine kinase ligand 5) (LERK-5) (HTK ligand) (HTK-L) (P52800)
Organism: Mus musculus (Mouse)
Function: Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
Subcellular location: Cell membrane {ECO:0000250|UniProtKB:P52799}; Single-pass type I membrane protein {ECO:0000255}. Cell junction, adherens junction {ECO:0000269|PubMed:30639848}.
Sequence: MAMARSRRDSVWKYCWGLLMVLCRTAISRSIVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKDQADRCTIKKENTPLLNCARPDQDVKFTIKFQEFSPNLWGLEFQKNKDYYIISTSNGSLEGLDNQEGGVCQTRAMKILMKVGQDASSAGSARNHGPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNLLGSEVALFAGIASGCIIFIVIIITLVVLLLKYRRRHRKHSPQHTTTLSLSTLATPKRGGNNNGSEPSDVIIPLRTADSVFCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV
InterPro domains: - IPR031328: Ephrin (family) [18-249]
- IPR001799: Ephrin receptor-binding domain (domain) [31-167]
- IPR008972: Cupredoxin (homologous_superfamily) [28-177]
- IPR019765: Ephrin, conserved site (conserved_site) [112-139]
- IPR034255: Ephrin-B ectodomain (domain) [32-168]
Interaction partners: - Ephrin type-B receptor 4
- Ephrin type-A receptor 4
- Ephrin type-B receptor 1
- Ephrin type-B receptor 2
- Ephrin type-B receptor 3
- Ephrin type-B receptor 6
- Ephrin type-A receptor 1
- Regulator of G-protein signaling 3
- Cytoplasmic protein NCK2
- Ephrin-B1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0005102 (signaling receptor binding), GO:0046875 (ephrin receptor binding)
Biological Process (BP): GO:0008150 (biological process), GO:0040011 (locomotion), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0002376 (immune system process), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0009605 (response to external stimulus), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0001775 (cell activation), GO:0007275 (multicellular organism development), GO:0048870 (cell motility), GO:0009653 (anatomical structure morphogenesis), GO:0042221 (response to chemical), GO:0045321 (leukocyte activation), GO:0042330 (taxis), GO:0008283 (cell population proliferation), GO:0051240 (positive regulation of multicellular organismal process), GO:0007389 (pattern specification process), GO:0002684 (positive regulation of immune system process), GO:0002682 (regulation of immune system process), GO:0071840 (cellular component organization or biogenesis), GO:0051239 (regulation of multicellular organismal process), GO:0031294 (lymphocyte costimulation), GO:0050794 (regulation of cellular process), GO:0048522 (positive regulation of cellular process), GO:0032989 (cellular component morphogenesis), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0030155 (regulation of cell adhesion), GO:0002694 (regulation of leukocyte activation), GO:0045785 (positive regulation of cell adhesion), GO:0009880 (embryonic pattern specification), GO:0046649 (lymphocyte activation), GO:0050867 (positive regulation of cell activation), GO:0000902 (cell morphogenesis), GO:0002696 (positive regulation of leukocyte activation), GO:0042127 (regulation of cell population proliferation), GO:0070661 (leukocyte proliferation), GO:0009888 (tissue development), GO:0009790 (embryo development), GO:0016043 (cellular component organization), GO:0006935 (chemotaxis), GO:0050865 (regulation of cell activation), GO:0031295 (T cell costimulation), GO:0008284 (positive regulation of cell population proliferation), GO:0016477 (cell migration), GO:0048864 (stem cell development), GO:0046651 (lymphocyte proliferation), GO:0030030 (cell projection organization), GO:0022409 (positive regulation of cell-cell adhesion), GO:0097485 (neuron projection guidance), GO:0022407 (regulation of cell-cell adhesion), GO:0032990 (cell part morphogenesis), GO:0051249 (regulation of lymphocyte activation), GO:0060485 (mesenchyme development), GO:0070663 (regulation of leukocyte proliferation), GO:0032943 (mononuclear cell proliferation), GO:0001667 (ameboidal-type cell migration), GO:0048666 (neuron development), GO:0022008 (neurogenesis), GO:0048863 (stem cell differentiation), GO:0000904 (cell morphogenesis involved in differentiation), GO:0051251 (positive regulation of lymphocyte activation), GO:0007399 (nervous system development), GO:0042110 (T cell activation), GO:0070665 (positive regulation of leukocyte proliferation), GO:0048762 (mesenchymal cell differentiation), GO:0048858 (cell projection morphogenesis), GO:0030182 (neuron differentiation), GO:0014033 (neural crest cell differentiation), GO:0048667 (cell morphogenesis involved in neuron differentiation), GO:0050670 (regulation of lymphocyte proliferation), GO:0050870 (positive regulation of T cell activation), GO:0050863 (regulation of T cell activation), GO:0032944 (regulation of mononuclear cell proliferation), GO:0120039 (plasma membrane bounded cell projection morphogenesis), GO:0120036 (plasma membrane bounded cell projection organization), GO:0090497 (mesenchymal cell migration), GO:0050671 (positive regulation of lymphocyte proliferation), GO:0042098 (T cell proliferation), GO:0032946 (positive regulation of mononuclear cell proliferation), GO:0007411 (axon guidance), GO:0031175 (neuron projection development), GO:1903037 (regulation of leukocyte cell-cell adhesion), GO:0048699 (generation of neurons), GO:1903039 (positive regulation of leukocyte cell-cell adhesion), GO:0014032 (neural crest cell development), GO:0042102 (positive regulation of T cell proliferation), GO:0048812 (neuron projection morphogenesis), GO:0061564 (axon development), GO:0042129 (regulation of T cell proliferation), GO:0007409 (axonogenesis), GO:0001755 (neural crest cell migration)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0030054 (cell junction), GO:0070161 (anchoring junction), GO:0005886 (plasma membrane), GO:0005911 (cell-cell junction), GO:0005912 (adherens junction)