Protein: Tektin-3 (A6H782)
Organism: Bos taurus (Bovine)
Function: Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia and flagellar axoneme. Forms filamentous polymers in the walls of ciliary and flagellar microtubules. Required for normal sperm mobility (By similarity).
Subcellular location: Cytoplasm, cytoskeleton, cilium axoneme {ECO:0000269|PubMed:34715025}. Cytoplasm, cytoskeleton, flagellum axoneme {ECO:0000269|PubMed:27883267, ECO:0000269|PubMed:37327785}. Cytoplasmic vesicle, secretory vesicle, acrosome outer membrane {ECO:0000269|PubMed:19478333, ECO:0000269|PubMed:26268136, ECO:0000269|PubMed:27883267}; Peripheral membrane protein {ECO:0000305}. Note=In spermatozoa, preferentially localizes to the flagella, but also found in the head (By similarity). In the sperm flagellum, localizes to the periaxonemal region where it associates with the mitochondrial sheath and outer dense fibers (By similarity). Not detected in the central axonemal region of the flagellum (By similarity). Associates with the acrosome membrane in the equatorial segment of the sperm head (PubMed:27883267). Also detected just below the plasma membrane in the post-acrosomal region where it might localize to the postacrosomal dense lamina (PubMed:27883267). However, other studies report little or no expression in the postacrosomal region (By similarity). Translocates from the postacrosomal region to the equatorial segment after sperm activation (PubMed:27883267). Retained in the postacromal region, but not the equatorial segment, following the acrosome reaction (PubMed:27883267). Some studies report strong expression in the anterior acrosomal cap region (PubMed:19478333, PubMed:26268136). However, other studies report little or no expression in the acrosomal cap (PubMed:27883267). {ECO:0000250|UniProtKB:Q4V8G8, ECO:0000250|UniProtKB:Q9BXF9, ECO:0000269|PubMed:19478333, ECO:0000269|PubMed:26268136, ECO:0000269|PubMed:27883267}.
Sequence: MELLGSTLTATYAHPRPTPTNFLPAISTMASTYRDRFPHYNLTHSLSLPWRPSTYYKAASNWPTLDPYCTRSQRVSESTMLPFVSNRTTLFTRYTPDDWYRSNLTNFQESNTSRHNSERLRVDTSRLIQDKYQQTRKTQADSTQNLGERVNDIGFWKSEIIHELDAMIGETNELTDIKKRLERALMETEAPLQVARECLFHREKRMGIDLVHDEVEKELLTEVDTILCCQERMKLYLDKAIAQLAANRAAQHELEKDLSDKQSAYRIDDKCHHLRNTSDGVSYFHGVERVDATVSVPESWAKFTDDNILRSQSERAASAKLRDDIQNVLVVTANEMWNQFNKVNLAFTNRIAETADAKNKIQTHLAKTLQEIFQTEMTIESIKKAIVEKSAFLKVAQTRLDERTRRPNIELCRDMAQLRLVNEVYEVDDTIQTLQQRLRDAEDTLQSLAHTKATLEHDLAVKANSLYIDQDKCMSMRRSFPSTLRLVGFC
InterPro domains: - IPR048256: Tektin-like (family) [99-481]
- IPR000435: Tektins (family) [85-488]
Interaction partners: - EF-hand domain-containing protein 1
- RIB43A-like with coiled-coils protein 2
- Tektin-2
- Outer dynein arm-docking complex subunit 4
- Tektin bundle-interacting protein 1
- EF-hand domain-containing family member C2
- Calaxin
- Outer dynein arm-docking complex subunit 1
- Tubulin beta-4B chain
- Tektin-4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0016043 (cellular component organization), GO:0044085 (cellular component biogenesis), GO:0006996 (organelle organization), GO:0030030 (cell projection organization), GO:0022607 (cellular component assembly), GO:0044782 (cilium organization), GO:0070925 (organelle assembly), GO:0030031 (cell projection assembly), GO:0120036 (plasma membrane bounded cell projection organization), GO:0060271 (cilium assembly), GO:0120031 (plasma membrane bounded cell projection assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0005930 (axoneme), GO:0005737 (cytoplasm), GO:0099080 (supramolecular complex), GO:0099081 (supramolecular polymer), GO:0005879 (axonemal microtubule), GO:0005881 (cytoplasmic microtubule), GO:0120025 (plasma membrane bounded cell projection), GO:0043229 (intracellular organelle), GO:0099568 (cytoplasmic region), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0099512 (supramolecular fiber), GO:0005929 (cilium), GO:0032838 (plasma membrane bounded cell projection cytoplasm), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0099513 (polymeric cytoskeletal fiber), GO:0097014 (ciliary plasm), GO:0005874 (microtubule), GO:0015630 (microtubule cytoskeleton)