Protein: Solute carrier family 22 member 6 (Organic anion transporter 1) (hOAT1) (PAH transporter) (hPAHT) (Renal organic anion transporter 1) (hROAT1) (Q4U2R8)
Organism: Homo sapiens (Human)
Function: Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the transport of prostaglandin E2 (PGE2) and prostaglandin F2-alpha (PGF2-alpha) and may be involved in their renal excretion. May contribute to the transport of organic compounds in testes across the blood-testis-barrier (Probable). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS) (By similarity). Also mediates the sodium-independent uptake of p-aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid (By similarity). PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate.
Subcellular location: Basolateral cell membrane {ECO:0000269|PubMed:9887087}; Multi-pass membrane protein {ECO:0000305}. Basal cell membrane {ECO:0000269|PubMed:35307651}; Multi-pass membrane protein {ECO:0000305}. Note=Localized to the basolateral membrane of renal proximal tubular cells (PubMed:9887087). Localized to the basal membrane of Sertoli cells (PubMed:35307651). {ECO:0000269|PubMed:35307651, ECO:0000269|PubMed:9887087}.
Sequence: MAFNDLLQQVGGVGRFQQIQVTLVVLPLLLMASHNTLQNFTAAIPTHHCRPPADANLSKNGGLEVWLPRDRQGQPESCLRFTSPQWGLPFLNGTEANGTGATEPCTDGWIYDNSTFPSTIVTEWDLVCSHRALRQLAQSLYMVGVLLGAMVFGYLADRLGRRKVLILNYLQTAVSGTCAAFAPNFPIYCAFRLLSGMALAGISLNCMTLNVEWMPIHTRACVGTLIGYVYSLGQFLLAGVAYAVPHWRHLQLLVSAPFFAFFIYSWFFIESARWHSSSGRLDLTLRALQRVARINGKREEGAKLSMEVLRASLQKELTMGKGQASAMELLRCPTLRHLFLCLSMLWFATSFAYYGLVMDLQGFGVSIYLIQVIFGAVDLPAKLVGFLVINSLGRRPAQMAALLLAGICILLNGVIPQDQSIVRTSLAVLGKGCLAASFNCIFLYTGELYPTMIRQTGMGMGSTMARVGSIVSPLVSMTAELYPSMPLFIYGAVPVAASAVTVLLPETLGQPLPDTVQDLESRWAPTQKEAGIYPRKGKQTRQQQEHQKYMVPLQASAQEKNGL
InterPro domains: - IPR036259: MFS transporter superfamily (homologous_superfamily) [110-517]
- IPR004749: Organic cation transport protein/SVOP (family) [12-515]
- IPR020846: Major facilitator superfamily domain (domain) [93-509]
- IPR005828: Major facilitator, sugar transporter-like (family) [136-512]
Interaction partners: - Solute carrier family 2, facilitated glucose transporter member 9
- Organic anion transporter 3
- Threonylcarbamoyl-AMP synthase
- Sodium-dependent phosphate transport protein 4
- Multidrug and toxin extrusion protein 1
- ATP-binding cassette sub-family C member 2
- Multidrug and toxin extrusion protein 2
- ATP-binding cassette sub-family C member 4
- Regulatory solute carrier protein family 1 member 1
- Sodium-dependent noradrenaline transporter
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity), GO:0005515 (protein binding), GO:0005319 (lipid transporter activity), GO:0022804 (active transmembrane transporter activity), GO:0008514 (organic anion transmembrane transporter activity), GO:0015291 (secondary active transmembrane transporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0051179 (localization), GO:0032501 (multicellular organismal process), GO:0051234 (establishment of localization), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0003008 (system process), GO:0006807 (nitrogen compound metabolic process), GO:0050877 (nervous system process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006810 (transport), GO:0071702 (organic substance transport), GO:0071705 (nitrogen compound transport), GO:0033013 (tetrapyrrole metabolic process), GO:0006811 (monoatomic ion transport), GO:0015698 (inorganic anion transport), GO:0050953 (sensory perception of light stimulus), GO:0006812 (monoatomic cation transport), GO:0006820 (monoatomic anion transport), GO:0007601 (visual perception)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0045177 (apical part of cell), GO:0016020 (membrane), GO:0005576 (extracellular region), GO:0005615 (extracellular space), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0098590 (plasma membrane region), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0070062 (extracellular exosome), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0043230 (extracellular organelle), GO:0016324 (apical plasma membrane), GO:0065010 (extracellular membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:1903561 (extracellular vesicle)