Protein: Thiosulfate sulfurtransferase GlpE (EC 2.8.1.1) (P0A6V5)
Organism: Escherichia coli (strain K12)
Function: Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide. The relatively low affinity of GlpE for both thiosulfate and cyanide suggests that these compounds are not the physiological substrates. Thioredoxin 1 or related dithiol proteins could instead be the physiological sulfur-acceptor substrate. Possible association with the metabolism of glycerol-phosphate remains to be elucidated.
Subcellular location: Cytoplasm.
Sequence: MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLTNDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPAEVAYGA
InterPro domains: - IPR050229: Thiosulfate sulfurtransferase GlpE (family) [3-99]
- IPR023695: Thiosulfate sulfurtransferase, bacterial (family) [2-105]
- IPR036873: Rhodanese-like domain superfamily (homologous_superfamily) [1-108]
- IPR001763: Rhodanese-like domain (domain) [7-105]
Interaction partners: - Rhomboid protease GlpG
- 3-mercaptopyruvate sulfurtransferase
- Sulfite reductase flavoprotein alpha-component
- Sulfite reductase hemoprotein beta-component
- Alkanesulfonate monooxygenase
- Putative cytochrome YdhU
- Phosphoadenosine 5'-phosphosulfate reductase
- Alpha-ketoglutarate-dependent taurine dioxygenase
- Glycogen phosphorylase
- Maltodextrin phosphorylase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016782 (transferase activity, transferring sulphur-containing groups), GO:0016783 (sulfurtransferase activity), GO:0004792 (thiosulfate sulfurtransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044282 (small molecule catabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0044248 (cellular catabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0034655 (nucleobase-containing compound catabolic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:1901136 (carbohydrate derivative catabolic process), GO:0019439 (aromatic compound catabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0009116 (nucleoside metabolic process), GO:0046700 (heterocycle catabolic process), GO:1901657 (glycosyl compound metabolic process), GO:0034656 (nucleobase-containing small molecule catabolic process), GO:0072527 (pyrimidine-containing compound metabolic process), GO:0009164 (nucleoside catabolic process), GO:1901658 (glycosyl compound catabolic process), GO:0006213 (pyrimidine nucleoside metabolic process), GO:0009119 (ribonucleoside metabolic process), GO:0072529 (pyrimidine-containing compound catabolic process), GO:0046131 (pyrimidine ribonucleoside metabolic process), GO:0046135 (pyrimidine nucleoside catabolic process), GO:0042454 (ribonucleoside catabolic process), GO:0046133 (pyrimidine ribonucleoside catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)