Protein: Proprotein convertase subtilisin/kexin type 4 (EC 3.4.21.-) (KEX2-like endoprotease 3) (Neuroendocrine convertase 3) (NEC 3) (Prohormone convertase 3) (P29121)
Organism: Mus musculus (Mouse)
Function: Proprotein convertase involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. In males, important for ADAM2 processing as well as other acrosomal proteins with roles in fertilization and critical for normal fertilization events such as sperm capacitation, acrosome reaction and binding of sperm to zona pellucida. Also plays a role in female fertility, involved in the regulation of trophoblast migration and placental development, may be through the proteolytical processing and activation of proteins such as IGF2 (By similarity). May also participate in folliculogenesis in the ovaries.
Subcellular location: Cytoplasmic vesicle, secretory vesicle, acrosome membrane {ECO:0000269|PubMed:16371590, ECO:0000269|PubMed:21080038, ECO:0000269|PubMed:21302280}.
Sequence: MRPSQTELWLGLTLTLALLAVRWASAQAPIYVSSWAVRVTKGYQEAERLARKFGFVNLGQIFPDDQYFHLRHRGVAQQSLTPHWGHRLRLKKDPKVRWFEQQTLRRRVKRSLVVPTDPWFSKQWYMNKEIQQDLNILKAWNQGLTGRGVVISILDDGIEKDHPDLWANYDPLASYDFNDYDPDPQPRYTPNDENRHGTRCAGEVSATANNGFCGAGVAFNARIGGVRMLDGAITDIVEAQSLSLQPQHIHIYSASWGPEDDGRTVDGPGLLTQEAFRRGVTKGRQGLGTLFIWASGNGGLHYDNCNCDGYTNSIHTLSVGSTTRQGRVPWYSEACASTFTTTFSSGVVTDPQIVTTDLHHQCTDKHTGTSASAPLAAGMIALALEANPLLTWRDLQHLVVRASRPAQLQAEDWRINGVGRQVSHHYGYGLLDAGLLVDLARVWLPTKPQKKCAIRVVHTPTPILPRMLVPKNVTACSDGSRRRLIRSLEHVQVQLSLSYSRRGDLEIFLTSPMGTRSTLVAIRPLDISGQGYNNWIFMSTHYWDEDPQGLWTLGLENKGYYFNTGTLYYYTLLLYGTAEDMTARPQAPQVTSRARACVQRDTEGLCQESHSPLSILAGLCLISSQQWWWLYSHPQQPVTEGQASCHPPVTPAAAA
InterPro domains: - IPR038466: Peptidase S8, pro-domain superfamily (homologous_superfamily) [23-110]
- IPR002884: P domain (domain) [446-580]
- IPR015500: Peptidase S8, subtilisin-related (family) [146-383]
- IPR023827: Peptidase S8, subtilisin, Asp-active site (active_site) [151-162]
- IPR022398: Peptidase S8, subtilisin, His-active site (active_site) [196-206]
- IPR023828: Peptidase S8, subtilisin, Ser-active site (active_site) [368-378]
- IPR000209: Peptidase S8/S53 domain (domain) [146-429]
- IPR036852: Peptidase S8/S53 domain superfamily (homologous_superfamily) [113-443]
- IPR032815: Peptidase S8, pro-domain (domain) [34-110]
- IPR034182: Kexin/furin catalytic domain (domain) [115-404]
- IPR008979: Galactose-binding-like domain superfamily (homologous_superfamily) [437-580]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0017171 (serine hydrolase activity), GO:0008233 (peptidase activity), GO:0008236 (serine-type peptidase activity), GO:0004175 (endopeptidase activity), GO:0004252 (serine-type endopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0065008 (regulation of biological quality), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0042445 (hormone metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0016486 (peptide hormone processing), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0010817 (regulation of hormone levels), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901142 (insulin metabolic process), GO:0006518 (peptide metabolic process), GO:0006508 (proteolysis), GO:0051604 (protein maturation), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0010467 (gene expression), GO:0043043 (peptide biosynthetic process), GO:0016485 (protein processing), GO:0140448 (signaling receptor ligand precursor processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0012505 (endomembrane system), GO:0005576 (extracellular region), GO:0005615 (extracellular space), GO:0043229 (intracellular organelle), GO:0043233 (organelle lumen), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0005788 (endoplasmic reticulum lumen)