Protein: Riboflavin kinase (EC 2.7.1.26) (ATP:riboflavin 5'-phosphotransferase) (Flavokinase) (Q969G6)
Organism: Homo sapiens (Human)
Function: Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.
Subcellular location: Cytoplasm {ECO:0000250}.
Sequence: MRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVVSIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELPEHLKIKEDNFFQVSKSKIMNGH
InterPro domains: - IPR023468: Riboflavin kinase (family) [1-148]
- IPR023465: Riboflavin kinase domain superfamily (homologous_superfamily) [2-148]
- IPR015865: Riboflavin kinase domain, bacterial/eukaryotic (domain) [1-131]
Interaction partners: - FAD synthase
- Tumor necrosis factor receptor superfamily member 1A
- Tartrate-resistant acid phosphatase type 5
- Flavin reductase
- Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
- Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
- Tumor necrosis factor receptor type 1-associated DEATH domain protein
- Cytochrome b-245 light chain
- Iodotyrosine deiodinase 1
- NADPH oxidase 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0042802 (identical protein binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0006793 (phosphorus metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0019693 (ribose phosphate metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0090407 (organophosphate biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0072527 (pyrimidine-containing compound metabolic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0009259 (ribonucleotide metabolic process), GO:0009123 (nucleoside monophosphate metabolic process), GO:0072528 (pyrimidine-containing compound biosynthetic process), GO:0006220 (pyrimidine nucleotide metabolic process), GO:0009117 (nucleotide metabolic process), GO:0046390 (ribose phosphate biosynthetic process), GO:1901293 (nucleoside phosphate biosynthetic process), GO:0009129 (pyrimidine nucleoside monophosphate metabolic process), GO:0009124 (nucleoside monophosphate biosynthetic process), GO:0009165 (nucleotide biosynthetic process), GO:0009260 (ribonucleotide biosynthetic process), GO:0006221 (pyrimidine nucleotide biosynthetic process), GO:0009161 (ribonucleoside monophosphate metabolic process), GO:0009218 (pyrimidine ribonucleotide metabolic process), GO:0009156 (ribonucleoside monophosphate biosynthetic process), GO:0009220 (pyrimidine ribonucleotide biosynthetic process), GO:0009130 (pyrimidine nucleoside monophosphate biosynthetic process), GO:0009173 (pyrimidine ribonucleoside monophosphate metabolic process), GO:0046049 (UMP metabolic process), GO:0009174 (pyrimidine ribonucleoside monophosphate biosynthetic process), GO:0006222 (UMP biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0012505 (endomembrane system), GO:0005576 (extracellular region), GO:0031090 (organelle membrane), GO:0030141 (secretory granule), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0060205 (cytoplasmic vesicle lumen), GO:0031983 (vesicle lumen), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0034774 (secretory granule lumen), GO:0042581 (specific granule), GO:0070820 (tertiary granule), GO:0070013 (intracellular organelle lumen), GO:0031982 (vesicle), GO:0035580 (specific granule lumen), GO:0097708 (intracellular vesicle), GO:1904724 (tertiary granule lumen)