Protein: Glutamyl aminopeptidase (EAP) (EC 3.4.11.7) (Aminopeptidase A) (AP-A) (BP-1/6C3 antigen) (CD antigen CD249) (P16406)
Organism: Mus musculus (Mouse)
Function: Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II.
Subcellular location: Cell membrane {ECO:0000250|UniProtKB:Q07075}; Single-pass type II membrane protein.
Sequence: MNFAEEEPSKKYCIKGKHVAIICGVVVAVGLIVGLSVGLTRSCEQDTTPAPSQPPPEASTALPPQDQNVCPDSEDESGEWKNFRLPDFINPVHYDLEVKALMEEDRYTGIVTISVNLSKPTRDLWLHIRETKITKLPELRRPSGEQVPIRRCFEYKKQEYVVIQAAEDLAATSGDSVYRLTMEFKGWLNGSLVGFYKTTYMEDGQIRSIAATDHEPTDARKSFPCFDEPNKKSTYSISIIHPKEYSALSNMPEEKSEMVDDNWKKTTFVKSVPMSTYLVCFAVHRFTAIERKSRSGKPLKVYVQPNQKETAEYAANITQAVFDYFEDYFAMEYALPKLDKIAIPDFGTGAMENWGLVTYRETNLLYDPLLSASSNQQRVASVVAHELVHQWFGNTVTMDWWDDLWLNEGFASFFEFLGVNHAEKDWQMLSQVLLEDVFPVQEDDSLMSSHPVVVTVSTPAEITSVFDGISYSKGASILRMLQDWITPEKFQKGCQIYLKKFQFANAKTSDFWDSLQEASNLPVKEVMDTWTSQMGYPVVTVSGRQNITQKRFLLDSKADPSQPPSELGYTWNIPVRWADNDNSRITVYNRLDKGGITLNANLSGDAFLKINPDHIGFYRVNYEGGTWDWIAEALSSNHTRFSAADRSSFIDDAFALARAQLLNYKIALNLTMYLKSEEDFLPWERVISSVSYIISMFEDDRELYPMIETYFQGQVKPVADLLGWQDTGSHITKLLRASILGFACKMGDREALGNASQLFDSWLKGSASIPVNLRLLVYRYGMQNSGNEAAWNYTLEQYQKTSLAQEKEKLLYGLASVKDVKLLARYLEMLKDPNIIKTQDVFTVIRYISYNSYGKTMAWNWIQLNWDYLVSRFTINDRYLGRIVTIAEPFNTELQLWQMQSFFAKYPNAGAGAKPREQVLETVKNNIEWLNVNRQSIREWFASLP
InterPro domains: - IPR001930: Peptidase M1, alanine aminopeptidase/leukotriene A4 hydrolase (family) [220-413]
- IPR024571: ERAP1-like C-terminal domain (domain) [607-924]
- IPR045357: Aminopeptidase N-like , N-terminal domain (domain) [91-278]
- IPR034016: Aminopeptidase N-type (family) [92-531]
- IPR050344: Peptidase M1 family aminopeptidases (family) [48-941]
- IPR014782: Peptidase M1, membrane alanine aminopeptidase (domain) [313-530]
- IPR042097: Aminopeptidase N-like , N-terminal domain superfamliy (homologous_superfamily) [68-286]
- IPR027268: Peptidase M4/M1, CTD superfamily (homologous_superfamily) [287-535]
Interaction partners: - Angiotensinogen
- Angiotensin-converting enzyme 2
- T-cell surface glycoprotein CD4
- Proteasome subunit beta type-11
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0008233 (peptidase activity), GO:0008238 (exopeptidase activity), GO:0004177 (aminopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:1901575 (organic substance catabolic process), GO:0043170 (macromolecule metabolic process), GO:0044248 (cellular catabolic process), GO:0051604 (protein maturation), GO:0006518 (peptide metabolic process), GO:0006508 (proteolysis), GO:1901565 (organonitrogen compound catabolic process), GO:0043171 (peptide catabolic process), GO:0010467 (gene expression), GO:0016485 (protein processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0042995 (cell projection), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0098862 (cluster of actin-based cell projections), GO:0005903 (brush border), GO:0098590 (plasma membrane region), GO:0120025 (plasma membrane bounded cell projection), GO:0005886 (plasma membrane), GO:0031526 (brush border membrane), GO:0031253 (cell projection membrane)