Protein: Penicillin-insensitive murein endopeptidase (EC 3.4.24.-) (D-alanyl-D-alanine-endopeptidase) (DD-endopeptidase) (P0C0T5)
Organism: Escherichia coli (strain K12)
Function: Murein endopeptidase that cleaves the D-alanyl-meso-2,6-diamino-pimelyl amide bond that connects peptidoglycan strands. Likely plays a role in the removal of murein from the sacculus and could also play a role in the integration of nascent murein strands into the sacculus.
Subcellular location: Periplasm {ECO:0000305|PubMed:15292190}.
Sequence: MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGCIVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPAGGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEIFSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPADSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI
InterPro domains: - IPR005073: Peptidase M74, penicillin-insensitive murein endopeptidase (family) [1-274]
- IPR009045: Peptidase M74/Hedgehog-like, zinc-binding domain superfamily (homologous_superfamily) [20-274]
Interaction partners: - Probable membrane transporter protein YfcA
- Chorismate synthase
- Ribosomal protein uL3 glutamine methyltransferase
- Multidrug resistance protein MdtK
- DNA gyrase subunit A
- Elongation factor P lysine hydroxylase
- Peptidoglycan L,D-endopeptidase MepK
- Uncharacterized protein YfcL
- L-glyceraldehyde 3-phosphate reductase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0008233 (peptidase activity), GO:0004175 (endopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009605 (response to external stimulus), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0048584 (positive regulation of response to stimulus), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0009607 (response to biotic stimulus), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0009893 (positive regulation of metabolic process), GO:0006950 (response to stress), GO:0051707 (response to other organism), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:1901564 (organonitrogen compound metabolic process), GO:0043207 (response to external biotic stimulus), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0019538 (protein metabolic process), GO:0006952 (defense response), GO:0060255 (regulation of macromolecule metabolic process), GO:1901700 (response to oxygen-containing compound), GO:0001817 (regulation of cytokine production), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0009891 (positive regulation of biosynthetic process), GO:0098542 (defense response to other organism), GO:0006793 (phosphorus metabolic process), GO:0002831 (regulation of response to biotic stimulus), GO:0031325 (positive regulation of cellular metabolic process), GO:0009617 (response to bacterium), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0001819 (positive regulation of cytokine production), GO:0080090 (regulation of primary metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0031347 (regulation of defense response), GO:0071219 (cellular response to molecule of bacterial origin), GO:0032496 (response to lipopolysaccharide), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051607 (defense response to virus), GO:0051246 (regulation of protein metabolic process), GO:0009751 (response to salicylic acid), GO:0002237 (response to molecule of bacterial origin), GO:0010628 (positive regulation of gene expression), GO:0006796 (phosphate-containing compound metabolic process), GO:0010468 (regulation of gene expression), GO:0036211 (protein modification process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0043412 (macromolecule modification), GO:0006954 (inflammatory response), GO:0033993 (response to lipid), GO:0042742 (defense response to bacterium), GO:0031326 (regulation of cellular biosynthetic process), GO:0051247 (positive regulation of protein metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:0031328 (positive regulation of cellular biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006468 (protein phosphorylation), GO:0006355 (regulation of DNA-templated transcription), GO:0018193 (peptidyl-amino acid modification), GO:0016310 (phosphorylation), GO:1902680 (positive regulation of RNA biosynthetic process), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)