Protein: Sorting nexin-18 (Sorting nexin-associated Golgi protein 1) (Q91ZR2)
Organism: Mus musculus (Mouse)
Function: Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Stimulates the GTPase activity of DNM2. Promotes DNM2 location at the plasma membrane. Together with DNM2, involved in autophagosome assembly by regulating trafficking from recycling endosomes of phospholipid scramblase ATG9A.
Subcellular location: Endomembrane system {ECO:0000250|UniProtKB:Q96RF0}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q96RF0}; Cytoplasmic side {ECO:0000250|UniProtKB:Q96RF0}. Endosome membrane {ECO:0000250|UniProtKB:Q96RF0}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q96RF0}; Cytoplasmic side {ECO:0000250|UniProtKB:Q96RF0}. Recycling endosome membrane {ECO:0000250|UniProtKB:Q96RF0}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q96RF0}; Cytoplasmic side {ECO:0000250|UniProtKB:Q96RF0}. Cell membrane {ECO:0000250|UniProtKB:Q96RF0}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q96RF0}; Cytoplasmic side {ECO:0000250|UniProtKB:Q96RF0}. Cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:Q96RF0}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q96RF0}; Cytoplasmic side {ECO:0000250|UniProtKB:Q96RF0}. Note=Localized at sites of endocytosis at the cell membrane. Detected on newly formed macropinosomes. Partially colocalized with clathrin and dynamin at the cell membrane. Transiently recruited to clathrin-coated pits at a late stage of clathrin-coated vesicle formation. {ECO:0000250|UniProtKB:Q96RF0}.
Sequence: MALRARALYDFKSENPGEISLREHEVLSLCSEQDIEGWLEGINSRGDRGLFPASYVQVIRAPEPGPPADGGPGAPARYANVPPGGFEPLPAAPPAAFPPLLQPQASPGSFQPPGAGFPYGGGALQPSPQQLYGGYQASLGSDDDWDDEWDDSSTVADEPGALGSGAYPDLDGSSSAGGGAAGRYRLSTRSDLSLGSRGVSAPPAPSVWSQELGHGEPQPQSLLHLRQVGRGGLRAGRGVRLREGWGQAVRGAGSYGPEWQENPYPFQCTIDDPTKQTKFKGMKSYISYKLVPTHTQVPVHRRYKHFDWLYARLAEKFPVISVPHLPEKQATGRFEEDFISKRRKGLIWWMNHMASHPVLAQCDVFQHFLTCPSSTDEKAWKQGKRKAEKDEMVGANFFLTLSTPPAAALDLQEVESKIDGFKCFTKKMDDSALQLNHTANEFARKQVTGFKKEYQKVGQSFRGLSQAFELDQQAFSVGLNQAIAFTGDAYDAIGELFAEQPRQDLDPVMDLLALYQGHLANFPDIIHVQKGALTKVKESRRHVEEGKMEVQKADGIQDRCNTISFATLAEIHHFHQIRVRDFKSQMQHFLQQQIIFFQKVTQKLEEALHKYDSV
InterPro domains: - IPR027267: AH/BAR domain superfamily (homologous_superfamily) [424-613]
- IPR036028: SH3-like domain superfamily (homologous_superfamily) [4-75]
- IPR014536: Sorting nexin 9 family (family) [1-612]
- IPR001452: SH3 domain (domain) [1-61]
- IPR019497: Sorting nexin protein, WASP-binding domain (domain) [376-612]
- IPR001683: Phox homology (domain) [263-376]
- IPR035703: SNX18, PX domain (domain) [266-393]
- IPR035557: Sorting nexin-18, SH3 domain (domain) [4-58]
- IPR036871: PX domain superfamily (homologous_superfamily) [249-381]
Interaction partners: - Sorting nexin-9
- Dynamin-2
- Sorting nexin-33
- Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0008289 (lipid binding), GO:0005515 (protein binding), GO:0005543 (phospholipid binding), GO:0035091 (phosphatidylinositol binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0065008 (regulation of biological quality), GO:0051641 (cellular localization), GO:0050794 (regulation of cellular process), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0033036 (macromolecule localization), GO:0048522 (positive regulation of cellular process), GO:0046907 (intracellular transport), GO:0006810 (transport), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0051130 (positive regulation of cellular component organization), GO:0051128 (regulation of cellular component organization), GO:0032970 (regulation of actin filament-based process), GO:0044089 (positive regulation of cellular component biogenesis), GO:0090066 (regulation of anatomical structure size), GO:0045184 (establishment of protein localization), GO:0044087 (regulation of cellular component biogenesis), GO:0032535 (regulation of cellular component size), GO:1902903 (regulation of supramolecular fiber organization), GO:0006886 (intracellular protein transport), GO:0043254 (regulation of protein-containing complex assembly), GO:0071705 (nitrogen compound transport), GO:0015031 (protein transport), GO:1902905 (positive regulation of supramolecular fiber organization), GO:0033043 (regulation of organelle organization), GO:0032956 (regulation of actin cytoskeleton organization), GO:0071702 (organic substance transport), GO:0008104 (protein localization), GO:0010638 (positive regulation of organelle organization), GO:0031334 (positive regulation of protein-containing complex assembly), GO:0051493 (regulation of cytoskeleton organization), GO:0032271 (regulation of protein polymerization), GO:0110053 (regulation of actin filament organization), GO:0030838 (positive regulation of actin filament polymerization), GO:0030832 (regulation of actin filament length), GO:0032273 (positive regulation of protein polymerization), GO:0051495 (positive regulation of cytoskeleton organization), GO:0008064 (regulation of actin polymerization or depolymerization), GO:0030833 (regulation of actin filament polymerization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0043229 (intracellular organelle), GO:0005938 (cell cortex), GO:0005886 (plasma membrane), GO:0043228 (non-membrane-bounded organelle), GO:0030863 (cortical cytoskeleton), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0030864 (cortical actin cytoskeleton), GO:0015629 (actin cytoskeleton)