Protein: Bifunctional protein FolD [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)] (P9WG81)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.
Sequence: MGAIMLDGKATRDEIFGDLKQRVAALDAAGRTPGLGTILVGDDPGSQAYVRGKHADCAKVGITSIRRDLPADISTATLNETIDELNANPDCTGYIVQLPLPKHLDENAALERVDPAKDADGLHPTNLGRLVLGTPAPLPCTPRGIVHLLRRYDISIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDLPALTRQADIVVAAVGVAHLLTADMVRPGAAVIDVGVSRTDDGLVGDVHPDVWELAGHVSPNPGGVGPLTRAFLLTNVVELAERR
InterPro domains: - IPR036291: NAD(P)-binding domain superfamily (homologous_superfamily) [122-279]
- IPR020630: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain (domain) [6-120]
- IPR020631: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (domain) [115-280]
- IPR046346: Aminoacid dehydrogenase-like, N-terminal domain superfamily (homologous_superfamily) [1-121]
- IPR000672: Tetrahydrofolate dehydrogenase/cyclohydrolase (family) [3-281]
Interaction partners: - Phosphoribosylglycinamide formyltransferase
- Bifunctional purine biosynthesis protein PurH
- Serine hydroxymethyltransferase 2
- Serine hydroxymethyltransferase 1
- Formyltetrahydrofolate deformylase
- Thymidylate synthase ThyA
- Methionyl-tRNA formyltransferase
- Aminomethyltransferase
- Flavin-dependent thymidylate synthase
- 5-formyltetrahydrofolate cyclo-ligase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0016491 (oxidoreductase activity), GO:0016645 (oxidoreductase activity, acting on the CH-NH group of donors), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0050660 (flavin adenine dinucleotide binding), GO:0016646 (oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor), GO:0017076 (purine nucleotide binding), GO:0030554 (adenyl nucleotide binding), GO:0050661 (NADP binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006790 (sulfur compound metabolic process), GO:0006082 (organic acid metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0000096 (sulfur amino acid metabolic process), GO:0044272 (sulfur compound biosynthetic process), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0000097 (sulfur amino acid biosynthetic process), GO:0046394 (carboxylic acid biosynthetic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0009066 (aspartate family amino acid metabolic process), GO:0006555 (methionine metabolic process), GO:0009067 (aspartate family amino acid biosynthetic process), GO:0009086 (methionine biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0071944 (cell periphery), GO:0005737 (cytoplasm), GO:0016020 (membrane), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0005886 (plasma membrane), GO:0009274 (peptidoglycan-based cell wall)