Protein: NEDD8-activating enzyme E1 regulatory subunit AXL (Protein AXR1-LIKE) (Protein AXR1-LIKE 1) (Q9ZV69)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Regulatory subunit of the dimeric ECR1-AXL1 E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1 (Probable). May function redundantly with AXR1 in the RUB conjugating pathway. Seems not to be functionally equivalent to AXR1 in vivo.
Subcellular location: Nucleus {ECO:0000250|UniProtKB:P42744}.
Sequence: MAEPKTKYDRQLRIWGELGQSALETASICLLNCGPTGSEALKNLVIGGIGSITIVDGSKVEIGDLGNNFMVDAKSVGQSRAKTVCGFLQELNDSVKANFVEENPDTLISTDPSFFSQFTLVIATQLVEDSMVKLDRICREANVMLVLARSYGLTGFVRISVKEHTAIETKPDHSLDDLRLNSPWPELKSYVESIDLNVEEPAAHKHIPYVVILVKVAEEWAQHHSGNLPSTREEKNEFKDLVKSKMVSADEENYKEALLAAFKVFAPTGISQEIQDINHDSCAEVGSNSSDFWVMVAALKEFISNEGGGEVPLEGSMPDMISSTEHYINLQKIYHSKAEADFLSMEQRVKSILVKVGQDPSSISKPTIKSFCKNARKLKVCRYRTIEDEFKSPSTTELHKYLADENYSGAIGFYILLRAVDRFAGTYKKFPGQFDGSTDEDASQLKTIALSLLSEMGCDGYELQEELYNEMCRFGAAEIHVVAALIGGITSQEVIKLITKQFVPKRGTFIFNGIDHKSQSLTL
InterPro domains: - IPR045886: ThiF/MoeB/HesA family (family) [1-516]
- IPR000594: THIF-type NAD/FAD binding fold (domain) [8-509]
- IPR035985: Ubiquitin-activating enzyme-like (homologous_superfamily) [4-521]
- IPR030667: NEDD8-activating enzyme E1 regulatory subunit APP-BP1 (family) [1-523]
Interaction partners: - NEDD8-activating enzyme E1 catalytic subunit
- Probable NEDD8-conjugating enzyme Ubc12-like
- NEDD8-conjugating enzyme Ubc12
- NEDD8-like protein RUB3
- F12F1.16 protein
- Ubiquitin-NEDD8-like protein RUB1
- NEDD8-specific protease 1
- Autophagy-related protein 3
- Cullin-1
- Molybdopterin synthase catalytic subunit
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0016874 (ligase activity), GO:0008641 (ubiquitin-like modifier activating enzyme activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0016877 (ligase activity, forming carbon-sulfur bonds)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0070647 (protein modification by small protein conjugation or removal), GO:0032446 (protein modification by small protein conjugation), GO:0045116 (protein neddylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)