Protein: Type 1 phosphatidylinositol 4,5-bisphosphate 4-phosphatase (Type 1 PtdIns-4,5-P2 4-Ptase) (EC 3.1.3.78) (PtdIns-4,5-P2 4-Ptase I) (Transmembrane protein 55B) (Q3TWL2)
Organism: Mus musculus (Mouse)
Function: Catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2) to phosphatidylinositol-4-phosphate (PtdIns-4-P) (By similarity). Does not hydrolyze phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-bisphosphate, inositol 3,5-bisphosphate, inositol 3,4-bisphosphate, phosphatidylinositol 5-monophosphate, phosphatidylinositol 4-monophosphate and phosphatidylinositol 3-monophosphate (By similarity). Regulates lysosomal positioning by recruiting JIP4 to lysosomal membranes, thus inducing retrograde transport of lysosomes along microtubules (By similarity). Contributes to assembly of the V-ATPase complex in lipid rafts of the lysosomal membrane and to subsequent amino acid-dependent activation of mTORC1. May play a role in the regulation of cellular cholesterol metabolism (By similarity).
Subcellular location: Late endosome membrane {ECO:0000269|PubMed:29378918}; Multi-pass membrane protein {ECO:0000255}. Lysosome membrane {ECO:0000269|PubMed:29644770}; Multi-pass membrane protein {ECO:0000255}. Cell membrane {ECO:0000269|PubMed:29378918}; Multi-pass membrane protein {ECO:0000255}. Cytoplasmic vesicle, phagosome membrane {ECO:0000269|PubMed:29378918}; Multi-pass membrane protein {ECO:0000255}.
Sequence: MAADGERSPLLSEAGDGGAGGNGLAGPGGSATGPGGGLTPSAPPYGAGKHAPPQAFPPFPEGHPAVLPGEDPPPYSPLTSPDSGSAPMITCRVCQSPINVEGKMHQHVVKCGVCNEATPIKNAPPGKKYVRCPCNCLLICKVTSQRIACPRPYCKRIINLGPVHPGPLSPEPQPMGVRVICGHCKNTFLWTEFTDRTLARCPHCRKVSSIGRRYPRKRCICCFLLGLLLAVTATGLAFGTWKHAQQYGGIYAAWAFVILLAVLCLGRALYWACMKVSHPVQNFS
InterPro domains: - IPR019178: Phosphatidylinositol 4,5-bisphosphate 4-phosphatase (family) [1-283]
Interaction partners: - C-Jun-amino-terminal kinase-interacting protein 4
- Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
- 1-phosphatidylinositol 3-phosphate 5-kinase
- Myotubularin
- Phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR2
- Phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR4
- Phosphatidylinositol polyphosphate 5-phosphatase type IV
- Synaptojanin-1
- Inositol polyphosphate 5-phosphatase OCRL
- Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0042578 (phosphoric ester hydrolase activity), GO:0016791 (phosphatase activity), GO:0052866 (phosphatidylinositol phosphate phosphatase activity), GO:0034593 (phosphatidylinositol bisphosphate phosphatase activity), GO:0034596 (phosphatidylinositol phosphate 4-phosphatase activity), GO:0106019 (phosphatidylinositol-4,5-bisphosphate phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0051234 (establishment of localization), GO:0051641 (cellular localization), GO:0023056 (positive regulation of signaling), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0033036 (macromolecule localization), GO:0048522 (positive regulation of cellular process), GO:1901615 (organic hydroxy compound metabolic process), GO:0010942 (positive regulation of cell death), GO:0006629 (lipid metabolic process), GO:0010941 (regulation of cell death), GO:0019637 (organophosphate metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0070727 (cellular macromolecule localization), GO:0010647 (positive regulation of cell communication), GO:0006793 (phosphorus metabolic process), GO:0043067 (regulation of programmed cell death), GO:0006644 (phospholipid metabolic process), GO:0043068 (positive regulation of programmed cell death), GO:2001235 (positive regulation of apoptotic signaling pathway), GO:0030258 (lipid modification), GO:0006796 (phosphate-containing compound metabolic process), GO:0008104 (protein localization), GO:2001233 (regulation of apoptotic signaling pathway), GO:0043065 (positive regulation of apoptotic process), GO:0033365 (protein localization to organelle), GO:0042981 (regulation of apoptotic process), GO:2001244 (positive regulation of intrinsic apoptotic signaling pathway), GO:0046839 (phospholipid dephosphorylation), GO:0046856 (phosphatidylinositol dephosphorylation), GO:0046488 (phosphatidylinositol metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005654 (nucleoplasm), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0005773 (vacuole), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0000323 (lytic vacuole), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0012506 (vesicle membrane), GO:0005770 (late endosome), GO:0005774 (vacuolar membrane), GO:0010008 (endosome membrane), GO:0031982 (vesicle), GO:0070013 (intracellular organelle lumen), GO:0030659 (cytoplasmic vesicle membrane), GO:0005764 (lysosome), GO:0097708 (intracellular vesicle), GO:0005634 (nucleus), GO:0031981 (nuclear lumen), GO:0031902 (late endosome membrane), GO:0098852 (lytic vacuole membrane), GO:0005765 (lysosomal membrane)