Protein: Carboxypeptidase E (CPE) (EC 3.4.17.10) (Carboxypeptidase H) (CPH) (Enkephalin convertase) (Prohormone-processing carboxypeptidase) (Q00493)
Organism: Mus musculus (Mouse)
Function: Sorting receptor that directs prohormones to the regulated secretory pathway. Acts also as a prohormone processing enzyme in neuro/endocrine cells, removing dibasic residues from the C-terminal end of peptide hormone precursors after initial endoprotease cleavage.
Subcellular location: Cytoplasmic vesicle, secretory vesicle {ECO:0000269|PubMed:16219686}. Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000250|UniProtKB:P15087}; Peripheral membrane protein {ECO:0000250|UniProtKB:P15087}. Secreted {ECO:0000250|UniProtKB:P15087}. Note=Associated with the secretory granule membrane through direct binding to lipid rafts in intragranular conditions. {ECO:0000269|PubMed:10896946}.
Sequence: MAGRGGRVLLALCAALVAGGWLLTAEAQEPGAPAAGMRRRRRLQQEDGISFEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVHEPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNLKKIVDQNSKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGTAHEYSSCPDDAIFQSLARAYSSFNPVMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPPEETLKSYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVDGIDHDVTSAKDGDYWRLLAPGNYKLTASAPGYLAITKKVAVPFSPAVGVDFELESFSERKEEEKEELMEWWKMMSETLNF
InterPro domains: - IPR057247: Zinc carboxypeptidases, zinc-binding region 2 (binding_site) [248-258]
- IPR008969: Carboxypeptidase-like, regulatory domain superfamily (homologous_superfamily) [375-471]
- IPR000834: Peptidase M14, carboxypeptidase A (domain) [52-465]
- IPR034232: Carboxypeptidase E, carboxypeptidase domain (domain) [53-371]
- IPR057246: Zinc carboxypeptidases, zinc-binding region 1 (binding_site) [105-127]
- IPR050753: Peptidase M14 domain-containing protein (family) [39-454]
Interaction partners: - Insulin-2
- Insulin-1
- Neuroendocrine convertase 2
- Secretogranin-3
- Pro-opiomelanocortin
- Chromogranin-A
- Neuroendocrine convertase 1
- Secretogranin-2
- ProSAAS
- Carboxypeptidase B
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0050839 (cell adhesion molecule binding), GO:0008233 (peptidase activity), GO:0005102 (signaling receptor binding), GO:0042043 (neurexin family protein binding), GO:0008238 (exopeptidase activity), GO:0004180 (carboxypeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0032501 (multicellular organismal process), GO:0048869 (cellular developmental process), GO:0050793 (regulation of developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0050794 (regulation of cellular process), GO:0051239 (regulation of multicellular organismal process), GO:0048523 (negative regulation of cellular process), GO:0000902 (cell morphogenesis), GO:2000026 (regulation of multicellular organismal development), GO:0060548 (negative regulation of cell death), GO:0010941 (regulation of cell death), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0048731 (system development), GO:0044057 (regulation of system process), GO:0048513 (animal organ development), GO:0022603 (regulation of anatomical structure morphogenesis), GO:0022008 (neurogenesis), GO:0007399 (nervous system development), GO:1901215 (negative regulation of neuron death), GO:0045995 (regulation of embryonic development), GO:0043069 (negative regulation of programmed cell death), GO:0043067 (regulation of programmed cell death), GO:0031644 (regulation of nervous system process), GO:1901214 (regulation of neuron death), GO:0060113 (inner ear receptor cell differentiation), GO:0051960 (regulation of nervous system development), GO:0030182 (neuron differentiation), GO:0048666 (neuron development), GO:0042981 (regulation of apoptotic process), GO:0043524 (negative regulation of neuron apoptotic process), GO:0048699 (generation of neurons), GO:0043066 (negative regulation of apoptotic process), GO:0043523 (regulation of neuron apoptotic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0005615 (extracellular space), GO:0005829 (cytosol), GO:0009986 (cell surface), GO:0071944 (cell periphery), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005576 (extracellular region), GO:0005794 (Golgi apparatus), GO:0120025 (plasma membrane bounded cell projection), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0043005 (neuron projection), GO:0043231 (intracellular membrane-bounded organelle), GO:0030424 (axon)