Protein: Phospholipid phosphatase 3 (EC 3.1.3.-) (EC 3.1.3.4) (Lipid phosphate phosphohydrolase 3) (PAP2-beta) (Phosphatidate phosphohydrolase type 2b) (Phosphatidic acid phosphatase 2b) (PAP-2b) (PAP2b) (Vascular endothelial growth factor and type I collagen-inducible protein) (VCIP) (O14495)
Organism: Homo sapiens (Human)
Function: Magnesium-independent phospholipid phosphatase of the plasma membrane that catalyzes the dephosphorylation of a variety of glycerolipid and sphingolipid phosphate esters including phosphatidate/PA, lysophosphatidate/LPA, diacylglycerol pyrophosphate/DGPP, sphingosine 1-phosphate/S1P and ceramide 1-phosphate/C1P. Also acts on N-oleoyl ethanolamine phosphate/N-(9Z-octadecenoyl)-ethanolamine phosphate, a potential physiological compound. Has both an extracellular and an intracellular phosphatase activity, allowing the hydrolysis and the cellular uptake of these bioactive lipid mediators from the milieu, regulating signal transduction in different cellular processes. Through the dephosphorylation of extracellular sphingosine-1-phosphate and the regulation of its extra- and intracellular availability, plays a role in vascular homeostasis, regulating endothelial cell migration, adhesion, survival, proliferation and the production of pro-inflammatory cytokines. By maintaining the appropriate levels of this lipid in the cerebellum, also ensure its proper development and function (By similarity). Through its intracellular lipid phosphatase activity may act in early compartments of the secretory pathway, regulating the formation of Golgi to endoplasmic reticulum retrograde transport carriers.
Subcellular location: Cell membrane {ECO:0000269|PubMed:12660161, ECO:0000269|PubMed:20123964, ECO:0000269|PubMed:9705349}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P97544}. Basolateral cell membrane {ECO:0000269|PubMed:14527693}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P97544}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:23591818}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P97544}. Endoplasmic reticulum-Golgi intermediate compartment membrane {ECO:0000269|PubMed:23591818}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P97544}. Golgi apparatus membrane {ECO:0000269|PubMed:23591818}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P97544}. Golgi apparatus, trans-Golgi network membrane {ECO:0000269|PubMed:23591818}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P97544}. Membrane raft {ECO:0000269|PubMed:17005594}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P97544}. Note=Cycles between the endoplasmic reticulum and the Golgi. {ECO:0000269|PubMed:23591818}.
Sequence: MQNYKYDKAIVPESKNGGSPALNNNPRRSGSKRVLLICLDLFCLFMAGLPFLIIETSTIKPYHRGFYCNDESIKYPLKTGETINDAVLCAVGIVIAILAIITGEFYRIYYLKKSRSTIQNPYVAALYKQVGCFLFGCAISQSFTDIAKVSIGRLRPHFLSVCNPDFSQINCSEGYIQNYRCRGDDSKVQEARKSFFSGHASFSMYTMLYLVLYLQARFTWRGARLLRPLLQFTLIMMAFYTGLSRVSDHKHHPSDVLAGFAQGALVACCIVFFVSDLFKTKTTLSLPAPAIRKEILSPVDIIDRNNHHNMM
InterPro domains: - IPR036938: Phosphatidic acid phosphatase type 2/haloperoxidase superfamily (homologous_superfamily) [40-274]
- IPR043216: Phosphatidic acid phosphatases-like (family) [36-274]
- IPR000326: Phosphatidic acid phosphatase type 2/haloperoxidase (domain) [130-276]
Interaction partners: - Phosphatidate phosphatase LPIN2
- Phosphatidate phosphatase LPIN1
- 1-acyl-sn-glycerol-3-phosphate acyltransferase beta
- Diacylglycerol O-acyltransferase 1
- Phosphatidate phosphatase LPIN3
- 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha
- Sphingosine kinase 1
- Sphingosine kinase 2
- Diacylglycerol O-acyltransferase 2
- Phospholipid phosphatase 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0044877 (protein-containing complex binding), GO:0005515 (protein binding), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0005102 (signaling receptor binding), GO:0050839 (cell adhesion molecule binding), GO:0005178 (integrin binding), GO:0042578 (phosphoric ester hydrolase activity), GO:0016791 (phosphatase activity), GO:0042577 (lipid phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006629 (lipid metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0006066 (alcohol metabolic process), GO:0043603 (amide metabolic process), GO:0006793 (phosphorus metabolic process), GO:0006644 (phospholipid metabolic process), GO:0019751 (polyol metabolic process), GO:0006643 (membrane lipid metabolic process), GO:0006672 (ceramide metabolic process), GO:0030258 (lipid modification), GO:0006796 (phosphate-containing compound metabolic process), GO:0006665 (sphingolipid metabolic process), GO:0016311 (dephosphorylation), GO:0046519 (sphingoid metabolic process), GO:0034311 (diol metabolic process), GO:0046839 (phospholipid dephosphorylation), GO:0006670 (sphingosine metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031984 (organelle subcompartment), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0031090 (organelle membrane), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0043231 (intracellular membrane-bounded organelle), GO:0005789 (endoplasmic reticulum membrane)