Protein: Myosin heavy chain kinase A (MHCK-A) (EC 2.7.11.7) (P42527)
Organism: Dictyostelium discoideum (Social amoeba)
Function: Catalyzes its autophosphorylation, which is needed for enzymatic activity and phosphorylates myosin II heavy chain at a threonine in the C-terminal tail region. This phosphorylation is critical for regulating the assembly and disassembly of myosin II filament, affecting myosin localization during an array of cellular contractile events, including cytokinesis and capping of cell surface receptors as well as chemotactic cell locomotion.
Sequence: MFNIKKRKESITGIPPINVNSPQSVPLSGTLQSPLITPNSPNFVSRQCPFKKFGCSSFLVSKAEFDNHLKDDAQFHLQLAVEKFDHQFDLHTQLMAHFTEQMEDQLEKTMKVVRNHTDSLGGNVQTKLDEGIEKCMAFAKKVEQQQQQLAKRLITQQIQEKKSTSSPLVKGGISGGGGSGGDDSFDGANISSMSTSKQELQQELQSLSIKMKKELTELSDELSQKLERSTGNIDIKIKRIEGEVNEKIDKRQLVSTIDDSIGKKTDSIGYTLESSIIKKVEEKEKKKSEQNQLLFDSKIESLKDKIKIIETQQLDTSSEVRKLKLESTSSGNLMAGLNGTSGRPSSSSHFIPSSVSAAANNINKNEIMEEVKKVEEKLQKKIREEIDNTKSELSKVERSVKDNRSEIEGLEKDCKNQFDKQDNKIKQVEDDLKKSDSLLLLMQNNLKKYNEFVDRERDRESERLKLQDSIKRLEQNQKKIEAEIQEGNEQVERVLREEASISPISSVPKSPITTKRSSIILNSPPMTSQQSSPKIQDLLSSSGSSSVSGINISSETGEMGILWEFDPIINKWIRLSMKLKVERKPFAEGALREAYHTVSLGVGTDENYPLGTTTKLFPPIEMISPISKNNEAMTQLKNGTKFVLKLYKKEAEQQASRELYFEDVKMQMVCRDWGNKFNQKKPPKKIEFLMSWVVELIDRSPSSNGQPILCSIEPLLVGEFKKNNSNYGAVLTNRSTPQAFSHFTYELSNKQMIVVDIQGVDDLYTDPQIHTPDGKGFGLGNLGKAGINKFITTHKCNAVCALLDLDVKLGGVLSGNNKKQLQQGTMVMPDILPELMPSDNTIKVGAKQLPKAEFSKKDLKCVSTIQSFRERVNSIAFFDNQKLLCAGYGDGTYRVFDVNDNWKCLYTVNGHRKSIESIACNSNYIFTSSPDNTIKVHIIRSGNTKCIETLVGHTGEVNCVVANEKYLFSCSYDKTIKVWDLSTFKEIKSFEGVHTKYIKTLALSGRYLFSGGNDQIIYVWDTETLSMLFNMQGHEDWVLSLHCTASYLFSTSKDNVIKIWDLSNFSCIDTLKGHWNSVSSCVVKDRYLYSGSEDNSIKVWDLDTLECVYTIPKSHSLGVKCLMVFNNQIISAAFDGSIKVWEWQSK
InterPro domains: - IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [551-805]
- IPR019775: WD40 repeat, conserved site (conserved_site) [967-1102]
- IPR001680: WD40 repeat (repeat) [858-1146]
- IPR020472: PAC1/LIS1-like, WD-40 repeat (repeat) [967-1102]
- IPR015943: WD40/YVTN repeat-like-containing domain superfamily (homologous_superfamily) [838-1146]
- IPR004166: Alpha-type protein kinase, alpha-kinase domain (domain) [564-808]
- IPR036322: WD40-repeat-containing domain superfamily (homologous_superfamily) [857-1144]
- IPR051852: Alpha-type Protein Kinase (family) [103-1100]
Interaction partners: - Myosin-2 heavy chain
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0016740 (transferase activity), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0140096 (catalytic activity, acting on a protein), GO:0097367 (carbohydrate derivative binding), GO:1901265 (nucleoside phosphate binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0004672 (protein kinase activity), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0032553 (ribonucleotide binding), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0032555 (purine ribonucleotide binding), GO:0017076 (purine nucleotide binding), GO:0005524 (ATP binding), GO:0032559 (adenyl ribonucleotide binding), GO:0004683 (calmodulin-dependent protein kinase activity), GO:0030554 (adenyl nucleotide binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0071840 (cellular component organization or biogenesis), GO:0030029 (actin filament-based process), GO:0042221 (response to chemical), GO:0016043 (cellular component organization), GO:1901700 (response to oxygen-containing compound), GO:0030036 (actin cytoskeleton organization), GO:0010033 (response to organic substance), GO:0031033 (myosin filament organization), GO:0014070 (response to organic cyclic compound), GO:0043933 (protein-containing complex organization), GO:1901654 (response to ketone), GO:0006996 (organelle organization), GO:0022411 (cellular component disassembly), GO:0097435 (supramolecular fiber organization), GO:0045472 (response to ether), GO:0007010 (cytoskeleton organization), GO:1903013 (response to differentiation-inducing factor 1), GO:0032984 (protein-containing complex disassembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0070938 (contractile ring), GO:0032153 (cell division site), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0005826 (actomyosin contractile ring), GO:0043229 (intracellular organelle), GO:0005938 (cell cortex), GO:0043228 (non-membrane-bounded organelle), GO:0030863 (cortical cytoskeleton), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0030864 (cortical actin cytoskeleton), GO:0015629 (actin cytoskeleton)