Protein: 3-isopropylmalate dehydratase small subunit 3 (EC 4.2.1.33) (2-(omega-methylthio)alkylmalate dehydratase small subunit 3) (EC 4.2.1.170) (AtLEUD2) (Isopropylmalate isomerase 1) (Isopropylmalate isomerase small subunit 3) (IPMI SSU3) (Methylthioalkylmalate isomerase small subunit) (MAM-IS) (Q9LYT7)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (Probable). Functions redundantly with LEUD1 in the methionine chain elongation pathway of aliphatic glucosinolate formation.
Subcellular location: Plastid, chloroplast stroma {ECO:0000269|PubMed:20663849}. Plastid {ECO:0000269|PubMed:32612621}.
Sequence: MATSQQFLNPTLFKSLASSNKNSCTLCPSPFLQLKSASTIFNYKPLTSSSATIITRVAASSSDSGESITRETFHGLCFVLKDNIDTDQIIPAEYGTLIPSIPEDREKLGSFALNGLPKFYNERFVVPGEMKSKYSVIIGGDNFGCGSSREHAPVCLGAAGAKAVVAESYARIFFRNCVATGEIFPLESEVRICDECKTGDVVTIEHKEDGSSLLINHTTRKEYKLKPLGDAGPVIDAGGIFAYARKAGMIPSA
InterPro domains: - IPR050075: 3-isopropylmalate dehydratase small subunit (family) [71-251]
- IPR015928: Aconitase/3-isopropylmalate dehydratase, swivel (homologous_superfamily) [69-249]
- IPR000573: Aconitase A/isopropylmalate dehydratase small subunit, swivel domain (domain) [135-187]
Interaction partners: - 3-isopropylmalate dehydrogenase 3, chloroplastic
- 3-isopropylmalate dehydrogenase 1, chloroplastic
- 3-isopropylmalate dehydratase large subunit, chloroplastic
- 3-isopropylmalate dehydrogenase 2, chloroplastic
- 2-isopropylmalate synthase 2, chloroplastic
- 2-isopropylmalate synthase 1, chloroplastic
- Methylthioalkylmalate synthase 1, chloroplastic
- Methylthioalkylmalate synthase 3, chloroplastic
- Methionine aminotransferase BCAT4
- 3-isopropylmalate dehydratase small subunit 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016829 (lyase activity), GO:0016835 (carbon-oxygen lyase activity), GO:0016836 (hydro-lyase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0016043 (cellular component organization), GO:0006520 (amino acid metabolic process), GO:0048229 (gametophyte development), GO:0006790 (sulfur compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0019757 (glycosinolate metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0019760 (glucosinolate metabolic process), GO:0016143 (S-glycoside metabolic process), GO:0044272 (sulfur compound biosynthetic process), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0009081 (branched-chain amino acid metabolic process), GO:1901657 (glycosyl compound metabolic process), GO:0019758 (glycosinolate biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0006996 (organelle organization), GO:1901605 (alpha-amino acid metabolic process), GO:0006551 (leucine metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:1901659 (glycosyl compound biosynthetic process), GO:0019761 (glucosinolate biosynthetic process), GO:0009082 (branched-chain amino acid biosynthetic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0016144 (S-glycoside biosynthetic process), GO:0009657 (plastid organization), GO:0046394 (carboxylic acid biosynthetic process), GO:0009658 (chloroplast organization), GO:0009098 (leucine biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0009532 (plastid stroma), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009570 (chloroplast stroma), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle)