Protein: Acetoacetyl-CoA synthetase (EC 6.2.1.16) (Q9JMI1)
Organism: Rattus norvegicus (Rat)
Function: Converts acetoacetate to acetoacetyl-CoA in the cytosol. Ketone body-utilizing enzyme, responsible for the synthesis of cholesterol and fatty acids.
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:10682835, ECO:0000269|PubMed:3082367}.
Sequence: MSKLARLEREEIMECQVMWEPDSKKDTQMDRFRAAVGTACGLALGNYDDLYHWSVRSYSDFWAEFWKFSGIVCSRMYDEVVDTSKGIADVPEWFRGSRLNYAENLLRHKENDRVALYVAREGREEIAKVTFEELRQQVALFAAAMRKMGVKKGDRVVGYLPNSAHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHGHLEKLQRVVKGLPDLQRVVLIPYVLPREKIDISKIPNSMFLDDFLASGTGAQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHVLHGNMTSSDILLYYTTVGWMMWNWMVSALATGASLVLYDGSPLVPTPNVLWDLVDRIGITILGTGAKWLSVLEEKDMKPMETHNLHTLHTILSTGSPLKAQSYEYVYRCIKSTVLLGSISGGTDIISCFMGQNSSIPVYKGEIQARNLGMAVEAWDEEGKTVWGASGELVCTKPIPCQPTHFWNDENGSKYRKAYFSKYPGVWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVEAFDEVEDSLCVPQYNRDGEERVVLFLKMASGHTFQPDLVKHIRDAIRLGLSARHVPSLILETQGIPYTINGKKVEVAVKQVIAGKTVEHRGAFSNPESLDLYRDIPELQDF
InterPro domains: - IPR042099: ANL, N-terminal domain (homologous_superfamily) [25-541]
- IPR005914: Acetoacetyl-CoA synthase (family) [16-671]
- IPR032387: Acetyl-coenzyme A synthetase, N-terminal domain (domain) [47-104]
- IPR000873: AMP-dependent synthetase/ligase domain (domain) [110-490]
- IPR045851: AMP-binding enzyme, C-terminal domain superfamily (homologous_superfamily) [543-663]
- IPR020845: AMP-binding, conserved site (conserved_site) [287-298]
Interaction partners: - Acetyl-CoA acetyltransferase, mitochondrial
- Hydroxymethylglutaryl-CoA synthase, cytoplasmic
- Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
- Hydroxymethylglutaryl-CoA lyase, mitochondrial
- Hydroxymethylglutaryl-CoA synthase, mitochondrial
- Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial
- Acetyl-CoA acetyltransferase, cytosolic
- Peroxisomal bifunctional enzyme
- Dehydrogenase/reductase SDR family member 6
- 3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0016877 (ligase activity, forming carbon-sulfur bonds), GO:0016878 (acid-thiol ligase activity), GO:0016405 (CoA-ligase activity), GO:0003987 (acetate-CoA ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006520 (amino acid metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006082 (organic acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0009064 (glutamine family amino acid metabolic process), GO:0006541 (glutamine metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)