Protein: DASH complex subunit DAD1 (A0A1D8PFP9)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Component of the DASH complex that connects microtubules with kinetochores and couples microtubule depolymerisation to chromosome movement; it is involved in retrieving kinetochores to the spindle poles before their re-orientation on the spindle in early mitosis and allows microtubule depolymerization to pull chromosomes apart and resist detachment during anaphase. Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and microtubules. Kinetochores also serve as an input point for the spindle assembly checkpoint, which delays anaphase until all chromosomes have bioriented on the mitotic spindle
Subcellular location: Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Nucleus
Sequence: MSNSSSNPSKNEYFIKQRDLLIQEISNNLSIVQTNLETLNRSLHESKQIGKEFDDVARLWSTFYDGMNGMNHQTRENTRDENNKISSSDTEDENNNNKI
InterPro domains: - IPR013958: DASH complex subunit Dad1 (family) [3-91]
Interaction partners: - DASH complex subunit DAM1
- DASH complex subunit DAD2
- DASH complex subunit SPC19
- DASH complex subunit SPC34
- DASH complex subunit DAD3
- DASH complex subunit DAD4
- Probable kinetochore protein NUF2
- DASH complex subunit ASK1
- Histone H3-like centromeric protein CSE4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0008092 (cytoskeletal protein binding), GO:0015631 (tubulin binding), GO:0008017 (microtubule binding), GO:0051010 (microtubule plus-end binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0022402 (cell cycle process), GO:0050794 (regulation of cellular process), GO:0007049 (cell cycle), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0051640 (organelle localization), GO:0051726 (regulation of cell cycle), GO:0007059 (chromosome segregation), GO:1903047 (mitotic cell cycle process), GO:0051656 (establishment of organelle localization), GO:0050000 (chromosome localization), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0008608 (attachment of spindle microtubules to kinetochore), GO:0032886 (regulation of microtubule-based process), GO:0051128 (regulation of cellular component organization), GO:0010564 (regulation of cell cycle process), GO:0000278 (mitotic cell cycle), GO:0051315 (attachment of mitotic spindle microtubules to kinetochore), GO:1902903 (regulation of supramolecular fiber organization), GO:0051984 (positive regulation of chromosome segregation), GO:0051983 (regulation of chromosome segregation), GO:0070507 (regulation of microtubule cytoskeleton organization), GO:0007346 (regulation of mitotic cell cycle), GO:0007088 (regulation of mitotic nuclear division), GO:1901989 (positive regulation of cell cycle phase transition), GO:0140014 (mitotic nuclear division), GO:0007080 (mitotic metaphase plate congression), GO:0033043 (regulation of organelle organization), GO:1905818 (regulation of chromosome separation), GO:1905820 (positive regulation of chromosome separation), GO:0006996 (organelle organization), GO:0000070 (mitotic sister chromatid segregation), GO:0051303 (establishment of chromosome localization), GO:1902099 (regulation of metaphase/anaphase transition of cell cycle), GO:0033048 (negative regulation of mitotic sister chromatid segregation), GO:0051783 (regulation of nuclear division), GO:0010965 (regulation of mitotic sister chromatid separation), GO:0045841 (negative regulation of mitotic metaphase/anaphase transition), GO:0033047 (regulation of mitotic sister chromatid segregation), GO:1901990 (regulation of mitotic cell cycle phase transition), GO:0051310 (metaphase plate congression), GO:0000819 (sister chromatid segregation), GO:0031110 (regulation of microtubule polymerization or depolymerization), GO:0032271 (regulation of protein polymerization), GO:1990758 (mitotic sister chromatid biorientation), GO:0031112 (positive regulation of microtubule polymerization or depolymerization), GO:0031113 (regulation of microtubule polymerization), GO:0048285 (organelle fission), GO:0033044 (regulation of chromosome organization), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0051276 (chromosome organization), GO:0000280 (nuclear division)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098687 (chromosomal region), GO:0000940 (outer kinetochore), GO:0140513 (nuclear protein-containing complex), GO:0099080 (supramolecular complex), GO:0042729 (DASH complex), GO:0000776 (kinetochore), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000775 (chromosome, centromeric region), GO:0043228 (non-membrane-bounded organelle), GO:0000779 (condensed chromosome, centromeric region), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome), GO:0005634 (nucleus), GO:0000793 (condensed chromosome)