Protein: Cytochrome P450 3A5 (EC 1.14.14.1) (CYPIIIA5) (Cytochrome P450-PCN3) (P20815)
Organism: Homo sapiens (Human)
Function: A cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of catechol estrogens from 17beta-estradiol (E2) and estrone (E1), namely 2-hydroxy E1 and E2. Catalyzes 6beta-hydroxylation of the steroid hormones testosterone, progesterone, and androstenedione. Catalyzes the oxidative conversion of all-trans-retinol to all-trans-retinal, a rate-limiting step for the biosynthesis of all-trans-retinoic acid (atRA). Further metabolizes all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may play a role in hepatic atRA clearance. Also involved in the oxidative metabolism of xenobiotics, including calcium channel blocking drug nifedipine and immunosuppressive drug cyclosporine.
Subcellular location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Sequence: MDLIPNLAVETWLLLAVSLVLLYLYGTRTHGLFKRLGIPGPTPLPLLGNVLSYRQGLWKFDTECYKKYGKMWGTYEGQLPVLAITDPDVIRTVLVKECYSVFTNRRSLGPVGFMKSAISLAEDEEWKRIRSLLSPTFTSGKLKEMFPIIAQYGDVLVRNLRREAEKGKPVTLKDIFGAYSMDVITGTSFGVNIDSLNNPQDPFVESTKKFLKFGFLDPLFLSIILFPFLTPVFEALNVSLFPKDTINFLSKSVNRMKKSRLNDKQKHRLDFLQLMIDSQNSKETESHKALSDLELAAQSIIFIFAGYETTSSVLSFTLYELATHPDVQQKLQKEIDAVLPNKAPPTYDAVVQMEYLDMVVNETLRLFPVAIRLERTCKKDVEINGVFIPKGSMVVIPTYALHHDPKYWTEPEEFRPERFSKKKDSIDPYIYTPFGTGPRNCIGMRFALMNMKLALIRVLQNFSFKPCKETQIPLKLDTQGLLQPEKPIVLKVDSRDGTLSGE
InterPro domains: - IPR036396: Cytochrome P450 superfamily (homologous_superfamily) [22-498]
- IPR017972: Cytochrome P450, conserved site (conserved_site) [434-443]
- IPR008072: Cytochrome P450, E-class, CYP3A (family) [10-491]
- IPR001128: Cytochrome P450 (family) [39-492]
- IPR002402: Cytochrome P450, E-class, group II (family) [123-406]
- IPR050705: Cytochrome P450 3A (family) [1-496]
Interaction partners: - UDP-glucuronosyltransferase 1A4
- UDP-glucuronosyltransferase 1A8
- UDP-glucuronosyltransferase 1A6
- UDP-glucuronosyltransferase 1A1
- UDP-glucuronosyltransferase 1A7
- UDP-glucuronosyltransferase 2B7
- UDP-glucuronosyltransferase 1A10
- Cytochrome P450 1A1
- Cytochrome P450 2B6
- Cytochrome P450 3A7
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0008289 (lipid binding), GO:0097159 (organic cyclic compound binding), GO:0043167 (ion binding), GO:0005515 (protein binding), GO:0016491 (oxidoreductase activity), GO:0005496 (steroid binding), GO:0004497 (monooxygenase activity), GO:0016725 (oxidoreductase activity, acting on CH or CH2 groups), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0019825 (oxygen binding), GO:0043169 (cation binding), GO:0019899 (enzyme binding), GO:0008395 (steroid hydroxylase activity), GO:0046872 (metal ion binding), GO:0046914 (transition metal ion binding), GO:0005506 (iron ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0051716 (cellular response to stimulus), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0009410 (response to xenobiotic stimulus), GO:0070887 (cellular response to chemical stimulus), GO:0006805 (xenobiotic metabolic process), GO:0071466 (cellular response to xenobiotic stimulus)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031984 (organelle subcompartment), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0031090 (organelle membrane), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0043231 (intracellular membrane-bounded organelle), GO:0005789 (endoplasmic reticulum membrane)