Protein: Gephyrin [Includes: Molybdopterin adenylyltransferase (MPT adenylyltransferase) (EC 2.7.7.75) (Domain G); Molybdopterin molybdenumtransferase (MPT Mo-transferase) (EC 2.10.1.1) (Domain E)] (Q8BUV3)
Organism: Mus musculus (Mouse)
Function: Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors.
Subcellular location: Postsynaptic cell membrane {ECO:0000250|UniProtKB:Q03555}; Lipid-anchor {ECO:0000250|UniProtKB:Q03555}; Cytoplasmic side {ECO:0000250|UniProtKB:Q03555}. Cell membrane {ECO:0000250|UniProtKB:Q03555}; Lipid-anchor {ECO:0000250|UniProtKB:Q03555}; Cytoplasmic side {ECO:0000250|UniProtKB:Q03555}. Cytoplasm, cytosol {ECO:0000269|PubMed:25025157}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q03555}. Cell projection, dendrite {ECO:0000250|UniProtKB:Q9NQX3}. Postsynaptic density {ECO:0000269|PubMed:27609886}. Note=Cytoplasmic face of glycinergic postsynaptic membranes (By similarity). Forms clusters at synapses (PubMed:25025157). {ECO:0000250|UniProtKB:Q03555, ECO:0000269|PubMed:25025157}.
Sequence: MATEGMILTNHDHQIRVGVLTVSDSCFRNLAEDRSGINLKDLVQDPSLLGGTISAYKIVPDEIEEIKETLIDWCDEKELNLILTTGGTGFAPRDVTPEATKEVIEREAPGMALAMLMGSLNVTPLGMLSRPVCGIRGKTLIINLPGSKKGSQECFQFILPALPHAIDLLRDAIVKVKEVHDELEDLPSPPPPLSPPPTTSPHKQTEDKGVQCEEEEEEKKDSGVASTEDSSSSHITAAALAAKIPDSIISRGVQVLPRDTASLSTTPSESPRAQATSRLSTASCPTPKQIRRPDESKGVASRVGSLKARLPSCSSTYSVSEVQSRCSSKENILRASHSAVDITKVARRHRMSPFPLTSMDKAFITVLEMTPVLGTEIINYRDGMGRVLAQDVYAKDNLPPFPASVKDGYAVRAADGPGDRFIIGESQAGEQPTQTVMPGQVMRVTTGAPIPCGADAVVQVEDTELIRESDDGTEELEVRILVQARPGQDIRPIGHDIKRGECVLAKGTHMGPSEIGLLATVGVTEVEVNKFPVVAVMSTGNELLNPEDDLLPGKIRDSNRSTLLATIQEHGYPTINLGIVGDNPDDLLNALNEGISRADVIITSGGVSMGEKDYLKQVLDIDLHAQIHFGRVFMKPGLPTTFATLDIDGVRKIIFALPGNPVSAVVTCNLFVVPALRKMQGILDPRPTIIKARLSCDVKLDPRPEYHRCILTWHHQEPLPWAQSTGNQMSSRLMSMRSANGLLMLPPKTEQYVELHKGEVVDVMVIGRL
InterPro domains: - IPR036425: MoaB/Mog-like domain superfamily (homologous_superfamily) [1-684]
- IPR008284: Molybdenum cofactor biosynthesis, conserved site (conserved_site) [81-643]
- IPR036135: MoeA, N-terminal and linker domain superfamily (homologous_superfamily) [352-529]
- IPR036688: MoeA, C-terminal, domain IV superfamily (homologous_superfamily) [687-769]
- IPR005110: MoeA, N-terminal and linker domain (domain) [358-522]
- IPR001453: MoaB/Mog domain (domain) [15-678]
- IPR005111: MoeA, C-terminal, domain IV (domain) [691-765]
- IPR038987: Molybdopterin biosynthesis protein MoeA-like (family) [350-769]
Interaction partners: - Rho guanine nucleotide exchange factor 9
- Glycine receptor subunit beta
- Neuroligin-2
- Neuronal acetylcholine receptor subunit alpha-3
- Vesicular inhibitory amino acid transporter
- Molybdopterin synthase catalytic subunit
- Glycine--tRNA ligase
- Gamma-aminobutyric acid receptor subunit gamma-2
- Gamma-aminobutyric acid receptor-associated protein
- Molybdenum cofactor biosynthesis protein 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0005515 (protein binding), GO:0016491 (oxidoreductase activity), GO:0016661 (oxidoreductase activity, acting on other nitrogenous compounds as donors), GO:0008940 (nitrate reductase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0051189 (prosthetic group metabolic process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0033036 (macromolecule localization), GO:0006793 (phosphorus metabolic process), GO:0010035 (response to inorganic substance), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0019637 (organophosphate metabolic process), GO:0019538 (protein metabolic process), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0044249 (cellular biosynthetic process), GO:0043545 (molybdopterin cofactor metabolic process), GO:0051668 (localization within membrane), GO:0043170 (macromolecule metabolic process), GO:0010038 (response to metal ion), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0090407 (organophosphate biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0032324 (molybdopterin cofactor biosynthetic process), GO:0008104 (protein localization), GO:0099173 (postsynapse organization), GO:0034330 (cell junction organization), GO:0061024 (membrane organization), GO:0072657 (protein localization to membrane), GO:0001941 (postsynaptic membrane organization), GO:0050808 (synapse organization), GO:0043113 (receptor clustering), GO:0072578 (neurotransmitter-gated ion channel clustering)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0030054 (cell junction), GO:0098794 (postsynapse), GO:0036477 (somatodendritic compartment), GO:0071944 (cell periphery), GO:0045211 (postsynaptic membrane), GO:0098590 (plasma membrane region), GO:0120025 (plasma membrane bounded cell projection), GO:0097447 (dendritic tree), GO:0045202 (synapse), GO:0005886 (plasma membrane), GO:0043005 (neuron projection), GO:0097060 (synaptic membrane), GO:0030425 (dendrite)