Protein: Baculoviral IAP repeat-containing protein 1b (Neuronal apoptosis inhibitory protein 2) (Q9QUK4)
Organism: Mus musculus (Mouse)
Function: Sensor component of the NLRC4 inflammasome that specifically recognizes and binds type III secretion system (T3SS) rod proteins such as S.typhimurium (Salmonella) PrgJ and B.thailandensis BsaK from pathogenic bacteria. Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri. Prevents motor-neuron apoptosis induced by a variety of signals.
Sequence: MAAQGEAVEEIICEFDDDLVSELSTLLRVDALSVLKRQQEEDHKTRMKMKKGFNSQMRSEAKRLKTFETYDKFRSWTPQEMAAAGFYHTGVKLGVQCFCCSLILFSTRLRKLPIENHKKLRPECEFLLGKDVGNIGKYDIRVKSPEKMLRGDKARYHEEEARLESFEDWPFYAHGTSPRVLSAAGFVFTGKRDTVQCFSCGGCLGNWEEGDDPWKEHAKWFPKCEFLQSKKSPEEITQYVQSYEGFLHVTGEHFVNSWVRRELPMVSAYCNDSVFANEELRMDTFKDWPHESPGAVEALVKAGLFYTGKRDIVQCFSCGGCMEKWAEGDNPIEDHTKFFPNCVFLQTLKSSAEVIPALQSHCALPEAMETTSESNHDDAAAVHSTVVDVSPSEAQELEPASSLVSVLCRDQDHSEAQGRGCASSGTYLPSTDLGQSEAQWLQEARSLSEQLRDTYTKATFRHMNLPEVYSSLGTDHLLSCDVSIISKHISQPVQGSLTIPEVFSNLNSVMCVEGEAGSGKTTFLKRIAFLWASGCCPLLYRFQLVFYLSLSSITPGQELAKIICAQLLGAGGCISEVCLSSIIQQLQHQVLFLLDDYSGLASLPQALHTLITKNYLSRTCLLIAVHTNKVRGIRPYLDTSLEIKEFPFYNTVSVLRKLFSHDIMRVRKFINYFGFHEELQGIHKTPLFVAAVCTDWFKNPSDQPFQDVALFKAYMQYLSLKHKGAAKPLQATVSSCGQLALTGLFSSCFEFNSDNLAEAGVDEDEELTTCLMSKFTAQRLRPVYRFLGPLFQEFLAAVRLTELLSSDRQEDQDLGLYYLRQINSPLKAMSIYHTFLKYVSSHPSSKAAPTVVSHLLQLVDEKESLENMSENEDYMKLHPEALLWIECLRGLWQLSPESFSLFISENLLRICLNFAHESNTVAACSPVILQFLRGRTLDLKVLSLQYFWDHPETLLLLKSIKISLNGNNWVQRIDFSLIEKSFEKVQPPTIDQDYAIAFQPINEVQKNLSEKKHIIKKYEDMKHQIPLNISTGYWKLSPKPYKIPKLEVQVTNTGPADQALLQVLMEVFSASQSIEFRLSDSSGFLESIRPALELSKASVTKCSMSRLELSREDQKLLLTLPTLQSLEVSETNQLPDQLFHNLHKFLGLKELCVRLDSKPDVLSVLPGEFPNLHHMEKLSIRTSTESDLSKLVKLIQNSPNLHVFHLKCNFLSNCEPLMTVLASCKKLREIEFSGRCFEAMTFVNILPNFVFLKILNLRDQQFPDKETSEKFAQALGSLRNLEKLFVPTGDGIHQVAKLIVRQCLQLPCLRVLVFAETLDDDSVLEIAKGATRGGFQKLENLDLTLNHKITEEGYRNFFQVLDNLPNLKNLDISRHIPECIQIQAITVKALGQCVSRLPSLTRLGMLSWLLDEEDIKVINDVKERHPQSKRLTVHWRWVVPFSPVIQK
InterPro domains: - IPR032675: Leucine-rich repeat domain superfamily (homologous_superfamily) [998-1425]
- IPR001370: BIR repeat (repeat) [58-347]
- IPR028789: Baculoviral IAP repeat-containing protein 1 (family) [1-1447]
- IPR053882: Nlrc4-like, winged helix domain (domain) [733-784]
- IPR007111: NACHT nucleoside triphosphatase (domain) [508-802]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [441-659]
- IPR040535: NLRC4, helical domain (domain) [810-916]
Interaction partners: - NLR family CARD domain-containing protein 4
- Baculoviral IAP repeat-containing protein 1e
- Caspase-1
- NACHT, LRR and PYD domains-containing protein 1a
- Interferon-inducible protein AIM2
- NACHT, LRR and PYD domains-containing protein 6
- Baculoviral IAP repeat-containing protein 1f
- NACHT, LRR and PYD domains-containing protein 3
- Gasdermin-D
- Apoptosis-associated speck-like protein containing a CARD
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0030234 (enzyme regulator activity), GO:0140678 (molecular function inhibitor activity), GO:0061134 (peptidase regulator activity), GO:0043028 (cysteine-type endopeptidase regulator activity involved in apoptotic process), GO:0004857 (enzyme inhibitor activity), GO:0061135 (endopeptidase regulator activity), GO:0030414 (peptidase inhibitor activity), GO:0043027 (cysteine-type endopeptidase inhibitor activity involved in apoptotic process), GO:0004866 (endopeptidase inhibitor activity), GO:0004869 (cysteine-type endopeptidase inhibitor activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0009719 (response to endogenous stimulus), GO:0008219 (cell death), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0065009 (regulation of molecular function), GO:0048523 (negative regulation of cellular process), GO:0009892 (negative regulation of metabolic process), GO:0050790 (regulation of catalytic activity), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0012501 (programmed cell death), GO:0060548 (negative regulation of cell death), GO:0010941 (regulation of cell death), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0044092 (negative regulation of molecular function), GO:0070887 (cellular response to chemical stimulus), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0071495 (cellular response to endogenous stimulus), GO:0060255 (regulation of macromolecule metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:0043086 (negative regulation of catalytic activity), GO:0032870 (cellular response to hormone stimulus), GO:0043069 (negative regulation of programmed cell death), GO:0051246 (regulation of protein metabolic process), GO:0043067 (regulation of programmed cell death), GO:0071310 (cellular response to organic substance), GO:0051336 (regulation of hydrolase activity), GO:0051248 (negative regulation of protein metabolic process), GO:0006915 (apoptotic process), GO:0043627 (response to estrogen), GO:0042981 (regulation of apoptotic process), GO:0051346 (negative regulation of hydrolase activity), GO:0030162 (regulation of proteolysis), GO:0071391 (cellular response to estrogen stimulus), GO:0052547 (regulation of peptidase activity), GO:0043066 (negative regulation of apoptotic process), GO:0045861 (negative regulation of proteolysis), GO:1904019 (epithelial cell apoptotic process), GO:0043154 (negative regulation of cysteine-type endopeptidase activity involved in apoptotic process), GO:0052548 (regulation of endopeptidase activity), GO:0010466 (negative regulation of peptidase activity), GO:1904036 (negative regulation of epithelial cell apoptotic process), GO:1904035 (regulation of epithelial cell apoptotic process), GO:0010951 (negative regulation of endopeptidase activity), GO:2000116 (regulation of cysteine-type endopeptidase activity), GO:2000117 (negative regulation of cysteine-type endopeptidase activity), GO:0043281 (regulation of cysteine-type endopeptidase activity involved in apoptotic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0061702 (inflammasome complex), GO:0005737 (cytoplasm)