Protein: Vacuolar protein sorting-associated protein 25 (AtVPS25) (ESCRT-II complex subunit VPS25) (Q8VZC9)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex (By similarity).
Subcellular location: Endosome {ECO:0000250}.
Sequence: MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLFSNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGLEDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV
InterPro domains: - IPR036390: Winged helix DNA-binding domain superfamily (homologous_superfamily) [7-177]
- IPR014041: ESCRT-II complex, Vps25 subunit, N-terminal winged helix (homologous_superfamily) [4-101]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [103-178]
- IPR008570: ESCRT-II complex, Vps25 subunit (family) [2-179]
Interaction partners: - Vacuolar protein sorting-associated protein 20 homolog 2
- Vacuolar protein sorting-associated protein 20 homolog 1
- Vacuolar protein sorting-associated protein 22 homolog 1
- Vacuolar protein sorting-associated protein 36
- E3 ubiquitin-protein ligase UPL4
- E3 ubiquitin-protein ligase UPL3
- Protein ELC
- Protein FREE1
- LisH domain-like protein
- Vacuolar protein sorting-associated protein 28 homolog 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0022414 (reproductive process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0003006 (developmental process involved in reproduction), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0009791 (post-embryonic development), GO:0048316 (seed development), GO:0009790 (embryo development), GO:0048608 (reproductive structure development), GO:0009793 (embryo development ending in seed dormancy), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0043170 (macromolecule metabolic process), GO:0048731 (system development), GO:0036211 (protein modification process), GO:0061458 (reproductive system development), GO:0043412 (macromolecule modification), GO:0010154 (fruit development), GO:0070647 (protein modification by small protein conjugation or removal), GO:0032446 (protein modification by small protein conjugation), GO:0016567 (protein ubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)