Protein: ARF guanine-nucleotide exchange factor 1 (P47102)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Activates the ARF proteins by exchanging bound GDP for free GTP. Plays a role in maintaining mitochondrial morphology, and in the turnover of mitochondria through mitophagy.
Subcellular location: Cytoplasm, cytosol {ECO:0000250|UniProtKB:P39993}. Membrane {ECO:0000250|UniProtKB:P39993}; Peripheral membrane protein {ECO:0000250|UniProtKB:P39993}. Endoplasmic reticulum {ECO:0000269|PubMed:25190516}. Mitochondrion {ECO:0000269|PubMed:25190516}. Note=Soluble and partially membrane-bound. {ECO:0000250|UniProtKB:P39993}.
Sequence: MHDVPMETVLAVNPATMIVKECINLCSAMNKQSRDKSQTSVAALLGGGSDIFLSQSDSFVDSFHNLPTSSYHDPLISGLVQLRLKINDLKGLDSLNALELLKPFLEIVSASSVSGYTTSLALDSLQKVFTLKIINKTFNDIQIAVRETVVALTHCRFEASKQISDDSVLLKVVTLLRDIITSSFGDYLSDTIIYDVLQTTLSLACNTQRSEVLRKTAEVTIAGITVKLFTKLKLLDPPTKTEKYINDESYTDNNLKDDIIGTTTSDNDLSSTDDDSAVADDNKNEKPVQQVIREQENDEETAEKAENVEPNYGITVIKDYLGLLLSLVMPENRMKHTTSAMKLSLQLINAAIEISGDKFPLYPRLFSLISDPIFKSVLFIIQSSTQYSLLQATLQLFTSLVVILGDYLPMQIELTLRRIFEILEDTTISGDVSKQKPPAIRELIIEQLSILWIHSPAFFLQLFVNFDCNLDRSDLSIDFIKELTKFSLPAAAVNTSNNIPPICLEGVLSLIENIYNDLQRFDRAEFVKNQKEIDILKQRDRKTEFILCVETFNEKAKKGIQMLIEKGFIDSDSNRDIASFLFLNNGRLNKKTIGLLLCDPKKTSLLKEFIDLFDFKGLRVDEAIRILLTKFRLPGESQQIERIVEAFSSKYSADQSNDKVELEDKKAGKNGSESMTEDDIIHVQPDADSVFVLSYSIIMLNTDSHNPQVKDHMTFDDYSNNLRGCYNGKDFPRWYLHKIYTSIKVKEIVMPEEHHGNERWFEDAWNNLISSTSVMTEMQRDFTNPISKLAQIDILQYEKAIFSNVRDIILKTLFKIFTVASSDQISLRILDAISKCTFINYYFSFDQSYNDTVLHLGEMTTLAQSSAKAVELDVDSIPLVEIFVEDTGSKISVSNQSIRLGQNFKAQLCTVLYFQIIKEISDPSIVSTRLWNQIVQLILKLFENLLMEPNLPFFTNFHSLLKLPELPLPDPDISIRKAKMSRSLLSTFASYLKGDEEPSEEDIDFSIKAFECVKASHPLSSVFENNQLVSPKMIETLLSSLVIEKTSENSPYFEQELLFLLEISIILISEASYGQEFGALIADHMINISNLDGLSKEAIARLASYKMFLVSRFDNPRDILSDLIEHDFLVKNEIFNTKYYESEWGKQVINDLFTHLNDVKYNERALKNVKFWNFLRILISAKDRQFAVYTFLEKYIQNGDIFVDDGNFMNILSLLDEMSCAGAVGTKWEQNYENSVEDGCEAPESNPYRSIIDLSSRSINITADLLSTVGRSNSALNKNEIIAAIQGLAHQCLNPCDELGMQALQALENILLSRASQLRTEKVAVDNLLETGLLPIFELDEIQDVKMKRITSILSVLSKIFLGQLVEGVTSNETFLRVLNVFNKYVDDPTVERQLQELIISKREIEKE
InterPro domains: - IPR035999: Sec7 domain superfamily (homologous_superfamily) [534-754]
- IPR000904: Sec7 domain (domain) [534-748]
- IPR023394: Sec7, C-terminal domain superfamily (homologous_superfamily) [614-754]
- IPR032691: Mon2/Sec7/BIG1-like, HUS domain (domain) [333-474]
Interaction partners: - ADP-ribosylation factor 1
- Coatomer subunit gamma
- ADP-ribosylation factor 2
- ARF guanine-nucleotide exchange factor 2
- ADP-ribosylation factor-like protein 1
- ADP-ribosylation factor 3
- Protein GMH1
- ADP-ribosylation factor guanine nucleotide-exchange factor SEC7
- ADP-ribosylation factor GTPase-activating protein GLO3
- ADP-ribosylation factor GTPase-activating protein GCS1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0030234 (enzyme regulator activity), GO:0140677 (molecular function activator activity), GO:0140678 (molecular function inhibitor activity), GO:0060589 (nucleoside-triphosphatase regulator activity), GO:0005085 (guanyl-nucleotide exchange factor activity), GO:0030695 (GTPase regulator activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0051179 (localization), GO:0051234 (establishment of localization), GO:0051641 (cellular localization), GO:0071840 (cellular component organization or biogenesis), GO:0030029 (actin filament-based process), GO:0044237 (cellular metabolic process), GO:0061919 (process utilizing autophagic mechanism), GO:0009056 (catabolic process), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0046907 (intracellular transport), GO:0030036 (actin cytoskeleton organization), GO:0044248 (cellular catabolic process), GO:0006810 (transport), GO:0006914 (autophagy), GO:0006888 (endoplasmic reticulum to Golgi vesicle-mediated transport), GO:0016236 (macroautophagy), GO:0016192 (vesicle-mediated transport), GO:0006996 (organelle organization), GO:0010256 (endomembrane system organization), GO:0033363 (secretory granule organization), GO:0007010 (cytoskeleton organization), GO:0016050 (vesicle organization), GO:0048193 (Golgi vesicle transport), GO:0006890 (retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum), GO:0006891 (intra-Golgi vesicle-mediated transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0031984 (organelle subcompartment), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0098791 (Golgi apparatus subcompartment), GO:0031985 (Golgi cisterna), GO:0043231 (intracellular membrane-bounded organelle), GO:0005795 (Golgi stack), GO:0000137 (Golgi cis cisterna)