Protein: MICAL-like protein 2 (Junctional Rab13-binding protein) (Molecule interacting with CasL-like 2) (MICAL-L2) (Q3TN34)
Organism: Mus musculus (Mouse)
Function: Effector of small Rab GTPases which is involved in junctional complexes assembly through the regulation of cell adhesion molecules transport to the plasma membrane and actin cytoskeleton reorganization. Regulates the endocytic recycling of occludins, claudins and E-cadherin to the plasma membrane and may thereby regulate the establishment of tight junctions and adherens junctions. In parallel, may regulate actin cytoskeleton reorganization directly through interaction with F-actin or indirectly through actinins and filamins. Most probably involved in the processes of epithelial cell differentiation, cell spreading and neurite outgrowth.
Subcellular location: Cell membrane; Peripheral membrane protein. Cell junction, tight junction {ECO:0000269|PubMed:18094055, ECO:0000269|PubMed:18332111, ECO:0000269|PubMed:23100251, ECO:0000269|PubMed:23890175}. Recycling endosome {ECO:0000269|PubMed:16525024}. Cell projection, neuron projection {ECO:0000269|PubMed:20008558}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:23100251, ECO:0000269|PubMed:23890175}.
Sequence: MAAIKALQEWCRQQCEGYRDVSITNMTTSFRDGLAFCAILHRHRPDLINFSALRKENIYENNKLAFQVAEEQLGIPALLDAEDMVALKVPDRLSILTYVSQYYNYFHGRSPIGGMAGIKRPSSDSTEELSGKKGLSQPAKLPSPAQTQRSPLSPARTNPVVQRNEGGSQRPSPKAAPGTAGSSVSSICGVCGKHVHLVQRHLADGRLYHRSCFRCKQCSSTLHSGAYRATGEPGVFVCTHHSSEVTSVSPKSSNLASRKPGGVTADTRPFGVSWTVQEANGEGTPLRVRTAAWEHAGGNTTAKGFVQTELKPPSTSQVHVGSSAGPKLPTITVTTTSVTSKALTHVTNSSPIGWSSPAQSSPANFNSRPVVSPSARNTHLPGSQGQTASKGVKTQLNLNSESSNTAVTPAWTSSASKTQQAREKFFQTPPSAPAPASAPAPAPTSKVPTVVTVPTSKVPNVVTAPTSKVPTVVTVPTSKVPTVVSAPTSKVPTVVSAPTSKVPTVVNSTNSRVTTVVNAPTSKVPTVVSATNGRVPTVVTAHTGRVPAVMNTSASKVSPVVDAPAQESSREQALSVLRKALPALTGSGTQAPNRSFPATSSVLVTLPKNEVPQKVPSDKLSALTTQTPNFTIKLEPSAPVNVGNTAVFLQAGKKSPSISPRVGKTSVGSRPQAEVAGVKGPGPISQEGQEEGPEGWRARLKPVDKKTPAGRSLEQKEPVLAEPRIGDTSRKASSSSDSSVHITLTSIQHKRKPCPAGSGPSPAALSPSPSHRKKLAVPPSLDVSADWLQPEPKKQEDGTRSCKEEKSPTRWSRERSAVLDSGLAPPGEAVTSPVRLHPDYIPQEELQRQLQDIESQLDALELRGVELEKRLRAAEGDASEDSLMVDWFRLIHEKQLLLRLESELMYKSKDQRLEEQQLDLQGELRRLMDKPEGLKSPQDRQREQELLSQYVNTVNDRSDIVDFLDEDRLREQEEDQMLENMIQNLGLQRKKSKSFLSKIWSSKSKSGQA
InterPro domains: - IPR036872: CH domain superfamily (homologous_superfamily) [1-108]
- IPR050540: F-actin Monooxygenase Mical (family) [3-984]
- IPR022735: bMERB domain (domain) [833-980]
- IPR001715: Calponin homology domain (domain) [1-107]
- IPR001781: Zinc finger, LIM-type (domain) [186-248]
Interaction partners: - Ras-related protein Rab-13
- Ras-related protein Rab-8A
- Alpha-actinin-4
- Ras-related protein Rab-8B
- Ras-related protein Rab-12
- Alpha-actinin-1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0008092 (cytoskeletal protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0048522 (positive regulation of cellular process), GO:0051050 (positive regulation of transport), GO:0032879 (regulation of localization), GO:0050794 (regulation of cellular process), GO:1903829 (positive regulation of protein localization), GO:0051049 (regulation of transport), GO:0051222 (positive regulation of protein transport), GO:0032388 (positive regulation of intracellular transport), GO:0060341 (regulation of cellular localization), GO:0090316 (positive regulation of intracellular protein transport), GO:0051223 (regulation of protein transport), GO:0032880 (regulation of protein localization), GO:1904951 (positive regulation of establishment of protein localization), GO:0070201 (regulation of establishment of protein localization), GO:0032386 (regulation of intracellular transport), GO:1903955 (positive regulation of protein targeting to mitochondrion), GO:1903747 (regulation of establishment of protein localization to mitochondrion), GO:0033157 (regulation of intracellular protein transport), GO:1903749 (positive regulation of establishment of protein localization to mitochondrion), GO:1903533 (regulation of protein targeting), GO:1903214 (regulation of protein targeting to mitochondrion)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)