Protein: Cell cycle checkpoint control protein RAD9A (mRAD9) (EC 3.1.11.2) (DNA repair exonuclease rad9 homolog A) (Rad9-like protein) (Q9Z0F6)
Organism: Mus musculus (Mouse)
Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity (By similarity).
Subcellular location: Nucleus {ECO:0000250|UniProtKB:Q99638}.
Sequence: MKCLITGGNVKVLGKAVHSLSRIGDELYLEPLKDGLSLRTVNSSRSAYACFLFAPLFFQQYQAASPGQDLLRCKILMKAFLSVFRSLAIVEKSVEKCCISLSGSHSHLVVQLHCKYGVKKTHNLSFQDCESLQAVFDPASCPHLLRTPARVLAEAVLSFPLALTEVTLGIGRGRRVILRSYQEEEADSTSKAMVTETSIGDEDFQQLHAPEGIAVTFCLKEFRGLLSFAESANLPLTIHFDVPGRPVIFTIEDSLLDAHFVLATLLEQDSCSQGPCSPKPHQPVPQKQAHSTPHLDDFTSDDIDCYMIAMETTGGNEGSGAQPSTSLPPVSLASHDLAPTSEEEAEPSTVPGTPPPKKFRSLFFGSILAPVHSPQGPNPVLAEDSDGEG
InterPro domains: - IPR046938: DNA clamp superfamily (homologous_superfamily) [6-128]
- IPR026584: Rad9 (family) [1-388]
- IPR007268: Rad9/Ddc1 (family) [1-388]
Interaction partners: - Cell cycle checkpoint protein RAD1
- Cell cycle checkpoint protein RAD17
- Checkpoint protein HUS1
- DNA topoisomerase 2-binding protein 1
- ATR-interacting protein
- Serine/threonine-protein kinase ATR
- Replication factor C subunit 2
- Serine-protein kinase ATM
- Replication factor C subunit 4
- Checkpoint protein HUS1B
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0019904 (protein domain specific binding), GO:0017124 (SH3 domain binding), GO:0004518 (nuclease activity), GO:0004527 (exonuclease activity), GO:0008408 (3'-5' exonuclease activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0000003 (reproduction), GO:0044237 (cellular metabolic process), GO:0051321 (meiotic cell cycle), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0007049 (cell cycle), GO:0051716 (cellular response to stimulus), GO:0071840 (cellular component organization or biogenesis), GO:1903046 (meiotic cell cycle process), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0061982 (meiosis I cell cycle process), GO:0051726 (regulation of cell cycle), GO:1901360 (organic cyclic compound metabolic process), GO:0140013 (meiotic nuclear division), GO:0006139 (nucleobase-containing compound metabolic process), GO:0035556 (intracellular signal transduction), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0016043 (cellular component organization), GO:0045786 (negative regulation of cell cycle), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0010948 (negative regulation of cell cycle process), GO:0044260 (cellular macromolecule metabolic process), GO:0010564 (regulation of cell cycle process), GO:0043170 (macromolecule metabolic process), GO:0007131 (reciprocal meiotic recombination), GO:0000075 (cell cycle checkpoint signaling), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0042770 (signal transduction in response to DNA damage), GO:1901987 (regulation of cell cycle phase transition), GO:0006259 (DNA metabolic process), GO:0006996 (organelle organization), GO:0007127 (meiosis I), GO:1901988 (negative regulation of cell cycle phase transition), GO:0031570 (DNA integrity checkpoint signaling), GO:0048285 (organelle fission), GO:0006310 (DNA recombination), GO:0006281 (DNA repair), GO:0000077 (DNA damage checkpoint signaling), GO:0000280 (nuclear division), GO:0035825 (homologous recombination), GO:0006302 (double-strand break repair), GO:0140527 (reciprocal homologous recombination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098687 (chromosomal region), GO:0140513 (nuclear protein-containing complex), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000781 (chromosome, telomeric region), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005694 (chromosome), GO:0005634 (nucleus), GO:0000228 (nuclear chromosome), GO:0000793 (condensed chromosome), GO:0000794 (condensed nuclear chromosome)