Protein: Putative pre-mRNA-splicing factor ATP-dependent RNA helicase C20H4.09 (Q9HE06)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA
Subcellular location: Nucleus
Sequence: MPNEFISQSLSTLTNTPLNIQKKLLPITKYRNQLLYAVEQNQITIVLGHTGCGKTTQIPQFLYEAGWASQNGIIGCTQPRRLVAKSVSERVSLELNSPPGSLCGYSIQFDHNVSEKTKIKYMTDGILLNEIFFDPLLERYSIVILDEVHERTLSTDLLLGVLKRILEKRNDFRLVLSSASVDANKLSQFFGQDKVCTMSIEGKLFPVETLFLQKPTENYVDSAIETVININSTYPPGDILVFLSGRKEIEYCIKKIEDSLIHASEDCQTLVPLPLHAGLTVDEQMRVFNIYDGDFRKVIFSTNIAETSITIDGIVYVVDSGFNKQRIYNPYTRTSKLINVPISKSSAIQRSGRAGRTMRGKVFRLYTEKAYSLMKEEFEADILNCDMSPLVLFLKGLGLKNILQFPFFVRPPTVHLMAALEDLYLLGVLDESGNLTDPLGIQISNSFLDANISKALLTSNQFGCTHEILSIASILTAGEVFYNPTSSSKNDAFVAHSSFFANEGDIITALNVFESFVGNKKDLQWCRKNYLNYQTLRQALDIRTHLVRFLNKFSIPTAQRLPSSDCSKILKCLLDGFVRNVAHLQNDGSYKTIGGKQVWLDSSSVLHEKKTPWIMYSSAVESETQIFVKNISKIESFWLDKYYKREK
InterPro domains: - IPR011545: DEAD/DEAH-box helicase domain (domain) [33-184]
- IPR001650: Helicase, C-terminal domain-like (domain) [219-398]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [23-210]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [3-605]
- IPR002464: DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site (conserved_site) [141-150]
- IPR048333: Helicase associated domain (HA2), winged-helix domain (domain) [419-444]
- IPR007502: Helicase-associated domain (domain) [418-510]
- IPR011709: DEAD-box helicase, OB fold (domain) [569-640]
Interaction partners: - Pre-mRNA-splicing factor cwf14
- Uncharacterized protein C20H4.06c
- Pre-mRNA-processing factor 17
- RNA-binding protein with serine-rich domain 1 homolog
- Pre-mRNA-processing factor 19
- Pre-mRNA-splicing factor cwf2
- Pre-mRNA-splicing factor 18
- Pre-mRNA-splicing factor slu7
- Pre-mRNA-splicing factor cwf3
- Pre-mRNA-splicing factor brr2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0004386 (helicase activity), GO:0008186 (ATP-dependent activity, acting on RNA), GO:0140098 (catalytic activity, acting on RNA), GO:0003724 (RNA helicase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0008380 (RNA splicing), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0000398 (mRNA splicing, via spliceosome), GO:0000375 (RNA splicing, via transesterification reactions), GO:0045292 (mRNA cis splicing, via spliceosome), GO:0000377 (RNA splicing, via transesterification reactions with bulged adenosine as nucleophile)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)