Protein: DNA repair nuclease APEX1 (A0MTA1)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Functions as an apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Has 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER (By similarity). May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation (By similarity). Required for passage through the mid-blastula transition MBT. May also act as an endoribonuclease involved in the control of single-stranded RNA metabolism (By similarity). Has no redox activity. Binds DNA and RNA (By similarity)
Subcellular location: Nucleus. Nucleus, nucleolus. Nucleus speckle. Endoplasmic reticulum. Cytoplasm. Mitochondrion
Sequence: MPKRAKKNEEGVDGEADNGTAAAKKEKKGKEPEAPILYEDPPEKLTSKDGRAANMKITSWNVDGLRAWVKKNGLDWVRKEDPDILCLQETKCAEKALPADITGMPEYPHKYWAGSEDKEGYSGVAMLCKTEPLNVTYGIGKEEHDKEGRVITAEFPDFFLVTAYVPNASRGLVRLDYRKTWDVDFRAYLCGLDARKPLVLCGDLNVAHQEIDLKNPKGNRKNAGFTPEEREGFTQLLEAGFTDSFRELYPDQAYAYTFWTYMMNARSKNVGWRLDYFVLSSALLPGLCDSKIRNTAMGSDHCPITLFLAV
InterPro domains: - IPR020848: AP endonuclease 1, conserved site (conserved_site) [269-280]
- IPR036691: Endonuclease/exonuclease/phosphatase superfamily (homologous_superfamily) [30-310]
- IPR005135: Endonuclease/exonuclease/phosphatase (domain) [59-301]
- IPR020847: AP endonuclease 1, binding site (binding_site) [82-91]
- IPR004808: AP endonuclease 1 (family) [41-310]
Interaction partners: - Probable endonuclease 4 isoform X1
- Endonuclease III-like protein 1
- Flap endonuclease 1
- DNA polymerase beta
- N-glycosylase/DNA lyase
- DNA repair protein XRCC1
- Adenine DNA glycosylase
- Proliferating cell nuclear antigen
- DNA ligase
- Poly [ADP-ribose] polymerase 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0016787 (hydrolase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0140097 (catalytic activity, acting on DNA), GO:0004518 (nuclease activity), GO:0004536 (deoxyribonuclease activity), GO:0004520 (DNA endonuclease activity), GO:0004519 (endonuclease activity), GO:0016894 (endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters), GO:0016889 (DNA endonuclease activity, producing 3'-phosphomonoesters)
Biological Process (BP): GO:0008150 (biological process), GO:0042592 (homeostatic process), GO:0019725 (cellular homeostasis), GO:0045454 (cell redox homeostasis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)