Protein: ATP-dependent helicase brm (EC 3.6.4.12) (Homeotic gene regulator) (Protein brahma) (P25439)
Organism: Drosophila melanogaster (Fruit fly)
Function: Transcriptional regulator. Acts as a coactivator, assisting one or more dedicated transcriptional activators of ANTC and BXC homeotic gene clusters. Can counteract the repressive effect of Polycomb protein. ATPase subunit of the Brahma complex, a multiprotein complex which is the equivalent of the yeast SWI/SNF complex and acts by remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. This complex can both serve as a transcriptional coactivator or corepressor, depending on the context. In type II neuroblast lineage, as part of the Brm remodeling complex, suppresses the formation of ectopic neuroblasts probably through interaction with erm and HDAC3.
Subcellular location: Nucleus.
Sequence: MASPSPANSPMPPPQAPSPMAPPSQSPAPSPHSPYPHQQPGPLQGPPPPGHPGAYGHPMQHGPPGQGPPGHHMPPHHQGMIFSKGPHMGMQMPPTGPNMSPYQTHGMPPNAPTQPCIVSPGGPPGGPPPPERSSQENLHALQRAIDSMEEKGLQEDPRYSQLLAMRATSKHQHLNGNQVNLLRTQITAYRLLARNKPISMQMQQALQAAQQQPPPGPPIGPPGAPGGPPPGSQHAGQPPVPPQQQQQPPPSAGTPPQCSTPPASNPYGPPVPGQKMQVAPPPPHMQQGQPLPPQPPQVGGPPPIQQQQPPQQQQQQSQPPPPEPHQHQLPNGGKPLSMGPSGGQPLIPSSPMQPQVRGTLPGMPPGSQVPQPGGGPQRQVPPAGMPMPKPNRITTVAKPVGLDPITLLQERENRIAARISLRMQELQRLPATMSEDLRLQAAIELRALRVLNFQRQLRMEFVQCTRRDTTLETALNIKLYKRTKRQGLREARATEKLEKQQKLEAERKRRQKHLEFLAAVLQHGKDLREFHRNNKAQLARMNKAVMNHHANAEREQKKEQERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAFLLSQTDEYISNLTQMVKQHKDDQMKKKEEEGKRLIQFKKELLMSGEYIGIDEGSIVADMRVHVVEQCTGKKLTGDDAPMLKHLHRWLNMHPGWDWIDDEEDSCGSNDDHKPKVEEQPTATEDATDKAQATGNDEDAKDLITKAKVEDDEYRTEEQTYYSIAHTIHEKVVEQASIMVNGTLKEYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTISLVTYLMDRKKVMGPYLIIVPLSTLPNWVLEFEKWAPAVGVVSYKGSPQGRRLLQNQMRATKFNVLLTTYEYVIKDKAVLAKIQWKYMIIDEGHRMKNHHCKLTQVLNTHYIAPYRLLLTGTPLQNKLPELWALLNFLLPSIFKSCSTFEQWFNAPFATTGEKVELNEEETILIIRRLHKVLRPFLLRRLKKEVEHQLPDKVEYIIKCDMSALQRVLYKHMQSKGVLLTDGSEKGKHGKGGAKALMNTIVQLRKLCNHPFMFQHIEEKYCDHTGGHGVVSGPDLYRVSGKFELLDRILPKLKATNHRVLLFCQMTQCMTIIEDYLGWRQFGYLRLDGTTKAEDRGELLRKFNAKGSDVFVFLLSTRAGGLGLNLQTADTVVIFDSDWNPHQDLQAQDRAHRIGQRNEVRVLRLMTVNSVEERILAAARYKLNMDEKVIQAGMFDQKSTGSERQQFLQTILHQDDNEEEEENEVPDDEMINMMIARSEEEIEIFKRMDAERKKEDEEIHPGRERLIDESELPDWLTKDDDEVERFHYQYDEDTILGRGSRQRKEVDYTDSLTEKEWLKAIDDGAEFDEEEEEDDSKRKRRKRKNRKEESDDDSLILKRRRRQNLDKRSKKQMHKIMSAVIKHNQDGRTLSEPFMKLPSRQRLPDYYEIIKRPVDIKKILQRIEDCKYADLNELEKDFMQLCQNAQIYNEEASLIYLDSIALQKVFVGARQRITAAADAAAVAAGDNTGEAHGNGGSDNSDNDDDDGGDDGSDDEEIATTSAAAVKMKLKLNKSLASAPATPTQSSSNVSSGAATTSKKQTRRKRSQKKYTISDDDDDDMD
InterPro domains: - IPR000330: SNF2, N-terminal (domain) [776-1070]
- IPR049730: SNF2/RAD5-like, C-terminal helicase domain (domain) [1096-1223]
- IPR038718: SNF2-like, N-terminal domain superfamily (homologous_superfamily) [766-1007]
- IPR014978: Glutamine-Leucine-Glutamine, QLQ (domain) [172-208]
- IPR006576: BRK domain (domain) [649-693]
- IPR018359: Bromodomain, conserved site (conserved_site) [1448-1505]
- IPR014012: Helicase/SANT-associated domain (domain) [501-573]
- IPR037259: BRK domain superfamily (homologous_superfamily) [636-714]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [746-1310]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [769-961]
- IPR036427: Bromodomain-like superfamily (homologous_superfamily) [1410-1537]
- IPR001650: Helicase, C-terminal domain-like (domain) [1099-1263]
- IPR029295: Snf2, ATP coupling domain (domain) [1307-1375]
- IPR001487: Bromodomain (domain) [1423-1532]
Interaction partners: - Brahma-associated protein of 60 kDa
- Brahma associated protein 111kD
- GH08712p
- Brahma associated protein 155 kDa
- Trithorax group protein osa
- Brahma associated protein 55kD
- Brahma associated protein 170kD
- Polybromo
- Supporter of activation of yellow protein
- Bromodomain containing 7/9
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0004386 (helicase activity), GO:1901363 (heterocyclic compound binding), GO:0008094 (ATP-dependent activity, acting on DNA), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0008134 (transcription factor binding), GO:0003676 (nucleic acid binding), GO:0003678 (DNA helicase activity), GO:0140097 (catalytic activity, acting on DNA), GO:0140297 (DNA-binding transcription factor binding), GO:0003677 (DNA binding), GO:0061629 (RNA polymerase II-specific DNA-binding transcription factor binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048869 (cellular developmental process), GO:0051641 (cellular localization), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0051640 (organelle localization), GO:0048522 (positive regulation of cellular process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0051646 (mitochondrion localization), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0016358 (dendrite development), GO:0048731 (system development), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0016043 (cellular component organization), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0022008 (neurogenesis), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0030030 (cell projection organization), GO:0007399 (nervous system development), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0090659 (walking behavior), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:0034643 (establishment of mitochondrion localization, microtubule-mediated), GO:0006996 (organelle organization), GO:0030182 (neuron differentiation), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0010468 (regulation of gene expression), GO:0048666 (neuron development), GO:0005739 (mitochondrion), GO:0031175 (neuron projection development), GO:1902680 (positive regulation of RNA biosynthetic process), GO:0048699 (generation of neurons), GO:2001141 (regulation of RNA biosynthetic process), GO:0120036 (plasma membrane bounded cell projection organization), GO:0006355 (regulation of DNA-templated transcription), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0045944 (positive regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000785 (chromatin), GO:1902494 (catalytic complex), GO:0140513 (nuclear protein-containing complex), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0070603 (SWI/SNF superfamily-type complex), GO:0043229 (intracellular organelle), GO:1904949 (ATPase complex), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0097346 (INO80-type complex), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0031011 (Ino80 complex), GO:0005694 (chromosome), GO:0005634 (nucleus), GO:0000228 (nuclear chromosome), GO:0005700 (polytene chromosome)