Protein: Ent-copalyl diphosphate synthase 3 (IeCPS3) (EC 5.5.1.13) (A0A3G1QTU5)
Organism: Isodon eriocalyx (Plectranthus eriocalyx)
Function: Involved in the biosynthesis of ent-kaurene diterpenoids natural products such as oridonin, miltiradiene, eriocalyxin B and nezukol, known to exhibit antitumor, anti-inflammatory and antibacterial activities, and in the production of gibberellins phytohormones. Catalyzes the conversion of (2E,6E,10E)-geranylgeranyl diphosphate (GGPP) to ent-copalyl diphosphate (ent-CPP).
Subcellular location: Plastid, chloroplast {ECO:0000255}.
Sequence: FRSTAAGRCLPVTCCVFPRHFRVSSSSILPSNAKVVGGCKKNRQIAVEAAQSLEVDSQQPMNQEEVSEKMRQLREKIRWMLQNMDDGEISVSPYDTAWVAMVEDIGGGGGPQFPTSLEWISNNQLDDGSWGDLRFLIYDRILNTLACVAVLTQWKMHLHKCQKGLRFIRENIDNLENGNDEMMPVGFEVAFPSLIQTAKKVGIKIPTDSPFMKNIYAKRDLKLRKIPMDILHTKPTTLLHSLEGMEGLDWEKLLNLRTDDGSFLMSPSSTAYVFRHTKDELCHQYLLKSVNKFNGGVPNVYPVDMFEHLWCVDRLQRLGISRYFQVEIQECLDYVYKYWTNKGICWARNTNVQDVDDTAMGFRLLRLHGYDVSTDAFKQFEKAGEFCSFPGQSTDALTGMYNLYRASQTMFNGEHILADAKEYSTNFLHKKRLANAIVDKWIITKDLPGEVGYALDVPFYASLPRLEARFFLEQYGGDDDVWIGKTLYRMLYVNCDTHLELAKLDYEKCQAVHQLEWESIQKWYRDWNLVEFGLSERSLLLAYYIAASTVFEPERSRERLAWAITAILVKTIASQRQLPLETKGESLGSILENEDGGRLIEFLINTIHQLSSEIVVAEGRDITQQLSNTWQKWLKTCKEGGDDDLGEAEARLIVHTLHLSSGLDESSFSHPKYHQLLEATSKVCGQLRLFQSRKQVDVDLATGTTFQIEAGMQELVKLVFTNSSEDLDSLTKQSFFSIARSFYYTAYCDEGAINSHIDKVLFEKID
InterPro domains: - IPR008930: Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid (homologous_superfamily) [81-480]
- IPR001906: Terpene synthase, N-terminal domain (domain) [249-455]
- IPR008949: Isoprenoid synthase domain superfamily (homologous_superfamily) [499-766]
- IPR050148: Terpene synthase-like (family) [71-764]
- IPR036965: Terpene synthase, N-terminal domain superfamily (homologous_superfamily) [76-506]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016853 (isomerase activity), GO:0016872 (intramolecular lyase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0120252 (hydrocarbon metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006720 (isoprenoid metabolic process), GO:0120251 (hydrocarbon biosynthetic process), GO:0042214 (terpene metabolic process), GO:0008299 (isoprenoid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0006721 (terpenoid metabolic process), GO:0016114 (terpenoid biosynthetic process), GO:0046246 (terpene biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle)