Protein: Neuroendocrine convertase 2 (NEC 2) (EC 3.4.21.94) (KEX2-like endoprotease 2) (Prohormone convertase 2) (Proprotein convertase 2) (PC2) (P16519)
Organism: Homo sapiens (Human)
Function: Serine endopeptidase which is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells.
Subcellular location: Cytoplasmic vesicle, secretory vesicle. Secreted {ECO:0000269|PubMed:28719828}. Note=Localized in the secretion granules.
Sequence: MKGGCVSQWKAAAGFLFCVMVFASAERPVFTNHFLVELHKGGEDKARQVAAEHGFGVRKLPFAEGLYHFYHNGLAKAKRRRSLHHKQQLERDPRVKMALQQEGFDRKKRGYRDINEIDINMNDPLFTKQWYLINTGQADGTPGLDLNVAEAWELGYTGKGVTIGIMDDGIDYLHPDLASNYNAEASYDFSSNDPYPYPRYTDDWFNSHGTRCAGEVSAAANNNICGVGVAYNSKVAGIRMLDQPFMTDIIEASSISHMPQLIDIYSASWGPTDNGKTVDGPRELTLQAMADGVNKGRGGKGSIYVWASGDGGSYDDCNCDGYASSMWTISINSAINDGRTALYDESCSSTLASTFSNGRKRNPEAGVATTDLYGNCTLRHSGTSAAAPEAAGVFALALEANLGLTWRDMQHLTVLTSKRNQLHDEVHQWRRNGVGLEFNHLFGYGVLDAGAMVKMAKDWKTVPERFHCVGGSVQDPEKIPSTGKLVLTLTTDACEGKENFVRYLEHVQAVITVNATRRGDLNINMTSPMGTKSILLSRRPRDDDSKVGFDKWPFMTTHTWGEDARGTWTLELGFVGSAPQKGVLKEWTLMLHGTQSAPYIDQVVRDYQSKLAMSKKEELEEELDEAVERSLKSILNKN
InterPro domains: - IPR038466: Peptidase S8, pro-domain superfamily (homologous_superfamily) [23-109]
- IPR002884: P domain (domain) [461-597]
- IPR015500: Peptidase S8, subtilisin-related (family) [158-397]
- IPR023827: Peptidase S8, subtilisin, Asp-active site (active_site) [163-174]
- IPR022398: Peptidase S8, subtilisin, His-active site (active_site) [208-218]
- IPR023828: Peptidase S8, subtilisin, Ser-active site (active_site) [382-392]
- IPR000209: Peptidase S8/S53 domain (domain) [158-445]
- IPR036852: Peptidase S8/S53 domain superfamily (homologous_superfamily) [113-459]
- IPR032815: Peptidase S8, pro-domain (domain) [33-109]
- IPR034182: Kexin/furin catalytic domain (domain) [122-418]
- IPR008979: Galactose-binding-like domain superfamily (homologous_superfamily) [453-599]
Interaction partners: - Neuroendocrine protein 7B2
- Insulin
- Carboxypeptidase E
- Thrombomodulin
- Pro-glucagon
- Secretogranin-1
- Somatostatin
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0017171 (serine hydrolase activity), GO:0042802 (identical protein binding), GO:0008233 (peptidase activity), GO:0008236 (serine-type peptidase activity), GO:0004175 (endopeptidase activity), GO:0004252 (serine-type endopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0065008 (regulation of biological quality), GO:0006807 (nitrogen compound metabolic process), GO:0042445 (hormone metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0016486 (peptide hormone processing), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0043603 (amide metabolic process), GO:0010817 (regulation of hormone levels), GO:0043170 (macromolecule metabolic process), GO:0006518 (peptide metabolic process), GO:0006508 (proteolysis), GO:0051604 (protein maturation), GO:0010467 (gene expression), GO:0016485 (protein processing), GO:0140448 (signaling receptor ligand precursor processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005615 (extracellular space), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0012505 (endomembrane system), GO:0005576 (extracellular region), GO:0043233 (organelle lumen), GO:0030141 (secretory granule), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0060205 (cytoplasmic vesicle lumen), GO:0031983 (vesicle lumen), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0034774 (secretory granule lumen), GO:0070013 (intracellular organelle lumen), GO:0031982 (vesicle), GO:0097708 (intracellular vesicle)