Protein: Ribonuclease HI (RNase HI) (EC 3.1.26.4) (Ribonuclease H) (RNase H) (P0A7Y4)
Organism: Escherichia coli (strain K12)
Function: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer. Involved in production of retron derived msDNA (a branched RNA linked by a 2',5'-phosphodiester bond to a single-stranded DNA). Strain K12 / MG1655 does not have retrons, however they can be expressed in this strain which requires this enzyme (tested with retron Ec86).
Subcellular location: Cytoplasm {ECO:0000305}.
Sequence: MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTNNRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVDLWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV
InterPro domains: - IPR022892: Ribonuclease HI (family) [2-149]
- IPR050092: Ribonuclease H (family) [5-145]
- IPR012337: Ribonuclease H-like superfamily (homologous_superfamily) [4-147]
- IPR036397: Ribonuclease H superfamily (homologous_superfamily) [1-155]
- IPR002156: Ribonuclease H domain (domain) [1-142]
Interaction partners: - DNA polymerase III subunit epsilon
- Ribonuclease HII
- DNA topoisomerase 1
- DNA polymerase I
- Crossover junction endodeoxyribonuclease RuvC
- Chromosomal replication initiator protein DnaA
- Pyrroline-5-carboxylate reductase
- ATP-dependent DNA helicase RecG
- Protein RecA
- Flap endonuclease Xni
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:0003676 (nucleic acid binding), GO:0140098 (catalytic activity, acting on RNA), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0004518 (nuclease activity), GO:0004540 (ribonuclease activity), GO:0004519 (endonuclease activity), GO:0004521 (RNA endonuclease activity), GO:0016891 (RNA endonuclease activity, producing 5'-phosphomonoesters), GO:0016893 (endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters), GO:0004523 (RNA-DNA hybrid ribonuclease activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0044248 (cellular catabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0034655 (nucleobase-containing compound catabolic process), GO:0009057 (macromolecule catabolic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0090304 (nucleic acid metabolic process), GO:0044265 (cellular macromolecule catabolic process), GO:0019439 (aromatic compound catabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0046700 (heterocycle catabolic process), GO:0006259 (DNA metabolic process), GO:0006308 (DNA catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0140535 (intracellular protein-containing complex), GO:1905348 (endonuclease complex), GO:1905347 (endodeoxyribonuclease complex)