Protein: Large neutral amino acids transporter small subunit 2 (L-type amino acid transporter 2) (mLAT2) (Solute carrier family 7 member 8) (Q9QXW9)
Organism: Mus musculus (Mouse)
Function: Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.
Subcellular location: Cell membrane {ECO:0000269|PubMed:28108384, ECO:0000269|PubMed:29355479, ECO:0000269|PubMed:31231240}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q9UHI5}. Basolateral cell membrane {ECO:0000269|PubMed:10574970}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q9UHI5}. Note=When coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi. {ECO:0000250|UniProtKB:Q9UHI5}.
Sequence: MEKGARQRNNTAKNHPGSDTSPEAEASSGGGGVALKKEIGLVSACGIIVGNIIGSGIFVSPKGVLENAGSVGLALIVWIVTGIITAVGALCYAELGVTIPKSGGDYSYVKDIFGGLAGFLRLWIAVLVIYPTNQAVIALTFSNYVLQPLFPTCFPPESGLRLLAAICLLLLTWVNCSSVRWATRVQDIFTAGKLLALALIIIMGIVQICKGEFFWLEPKNAFENFQEPDIGLVALAFLQGSFAYGGWNFLNYVTEELVDPYKNLPRAIFISIPLVTFVYVFANIAYVTAMSPQELLASNAVAVTFGEKLLGVMAWIMPISVALSTFGGVNGSLFTSSRLFFAGAREGHLPSVLAMIHVKRCTPIPALLFTCLSTLLMLVTSDMYTLINYVGFINYLFYGVTVAGQIVLRWKKPDIPRPIKVSLLFPIIYLLFWAFLLIFSLWSEPVVCGIGLAIMLTGVPVYFLGVYWQHKPKCFNDFIKSLTLVSQKMCVVVYPQEGNSGAEETTDDLEEQHKPIFKPTPVKDPDSEEQP
InterPro domains: - IPR002293: Amino acid/polyamine transporter I (family) [39-442]
- IPR004760: L-type amino acid transporter (family) [5-497]
- IPR050598: Cellular Amino Acid Transporter (family) [31-499]
Interaction partners: - Amino acid transporter heavy chain SLC3A2
- Large neutral amino acids transporter small subunit 4
- Large neutral amino acids transporter small subunit 3
- Amino acid transporter heavy chain SLC3A1
- Monocarboxylate transporter 10
- Neutral amino acid transporter B
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity), GO:0005515 (protein binding), GO:0008514 (organic anion transmembrane transporter activity), GO:0005342 (organic acid transmembrane transporter activity), GO:0046943 (carboxylic acid transmembrane transporter activity), GO:0015171 (amino acid transmembrane transporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0051234 (establishment of localization), GO:0006810 (transport), GO:0071702 (organic substance transport), GO:0071705 (nitrogen compound transport), GO:0006865 (amino acid transport), GO:0015849 (organic acid transport), GO:0015711 (organic anion transport), GO:0046942 (carboxylic acid transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0042995 (cell projection), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0098862 (cluster of actin-based cell projections), GO:0005903 (brush border), GO:0098590 (plasma membrane region), GO:0120025 (plasma membrane bounded cell projection), GO:0005886 (plasma membrane), GO:0031526 (brush border membrane), GO:0031253 (cell projection membrane)