Protein: Geminin (O88513)
Organism: Mus musculus (Mouse)
Function: Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Inhibits histone acetyltransferase activity of KAT7/HBO1 in a CDT1-dependent manner, inhibiting histone H4 acetylation and DNA replication licensing (By similarity). Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control (By similarity).
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:O75496}. Nucleus {ECO:0000250|UniProtKB:O75496}. Note=Mainly cytoplasmic but can be relocalized to the nucleus. {ECO:0000250|UniProtKB:O75496}.
Sequence: MNLSMKQKQEGAQENVKNSPVPRRTLKMIQPSADGSLVGRENELPKGLFKRKLWDDQLASQTSSCGPEANENKDVGDLTQEAFDLISKENPSSQYWKEVAEQRRKALYEALKENEKLHKEIEQKDSEIARLRKENKDLAEVAEHVQYMAEVIERLSNEPLDNFESPDSQEFDSEEEAVEYSELEDSGAGTCAEETVSSSTDARPCT
InterPro domains: - IPR022786: Geminin/Multicilin (family) [1-180]
Interaction partners: - DNA replication factor Cdt1
- DNA replication licensing factor MCM6
- Cell division control protein 6 homolog
- Origin recognition complex subunit 6
- Origin recognition complex subunit 4
- DNA replication licensing factor MCM3
- DNA replication licensing factor MCM5
- Multicilin
- DNA replication licensing factor MCM4
- Cell division control protein 45 homolog
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0005488 (binding), GO:0140677 (molecular function activator activity), GO:0003682 (chromatin binding), GO:0005515 (protein binding), GO:0140678 (molecular function inhibitor activity), GO:0019899 (enzyme binding), GO:0042826 (histone deacetylase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0019222 (regulation of metabolic process), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0009892 (negative regulation of metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0065009 (regulation of molecular function), GO:0048523 (negative regulation of cellular process), GO:0044238 (primary metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0044093 (positive regulation of molecular function), GO:0044085 (cellular component biogenesis), GO:0046483 (heterocycle metabolic process), GO:0044786 (cell cycle DNA replication), GO:0060255 (regulation of macromolecule metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0051098 (regulation of binding), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0051726 (regulation of cell cycle), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0045786 (negative regulation of cell cycle), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0016043 (cellular component organization), GO:0080090 (regulation of primary metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0007346 (regulation of mitotic cell cycle), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051052 (regulation of DNA metabolic process), GO:0033260 (nuclear DNA replication), GO:0010468 (regulation of gene expression), GO:0022607 (cellular component assembly), GO:0051099 (positive regulation of binding), GO:0051053 (negative regulation of DNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0035561 (regulation of chromatin binding), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0006259 (DNA metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0043933 (protein-containing complex organization), GO:0051253 (negative regulation of RNA metabolic process), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0035563 (positive regulation of chromatin binding), GO:0065003 (protein-containing complex assembly), GO:0071824 (protein-DNA complex subunit organization), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0006260 (DNA replication), GO:0006275 (regulation of DNA replication), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0008156 (negative regulation of DNA replication), GO:0006261 (DNA-templated DNA replication), GO:2000104 (negative regulation of DNA-templated DNA replication), GO:0030174 (regulation of DNA-templated DNA replication initiation), GO:0045892 (negative regulation of DNA-templated transcription), GO:0090329 (regulation of DNA-templated DNA replication), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0065004 (protein-DNA complex assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0005667 (transcription regulator complex), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0017053 (transcription repressor complex), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)