Protein: Sex-determining protein fem-1 (Feminization of XX and XO animals protein 1) (P17221)
Organism: Caenorhabditis elegans
Function: Substrate-recognition component of the cullin-RING-based CBC(fem-1) (Cul2-ElonginB-ElonginC) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (By similarity). The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms (By similarity). Sex-determining protein; essential for the adoption of the male sexual fate in all tissues. The CBC(fem-1) complex mediates the ubiquitination and subsequent proteasomal degradation of tra-1. Promotes ubiquitination and degradation of cdl-1. May be involved in turnover of neuronal substrates, thereby impacting synaptic acetylcholine release and neuromuscular transmission.
Subcellular location: Cytoplasm {ECO:0000305}.
Sequence: MTPNGHHFRTVIYNAAAVGNLQRIKVFTINSRNDRQWIIDCFNSDQDGRYPLVIAARNGHANVVEYLLEIGADPSVRGVVEFDNENIQGTPPLWAASAAGHIEIVKLLIEKANADVNQATNTRSTPLRGACYDGHLDIVKYLLEKGADPHIPNRHGHTCLMIASYRNKVGIVEELLKTGIDVNKKTERGNTALHDAAESGNVEVVKILLKHGSVLMKDIQGVDPLMGAALSGFLDVLNVLADQMPSGIHKRDALKLLGSTLLDKKMDAMSAMNCWKQSMEVPLHADDLRLVREMETFFEPQEVYEYQREAQNIAQVELLDGNIEAQRMQALVIRERILGGAHTDVHYYLRFRGAVYCDMGQMNRCYDLWKHALELQQKHFAPLYYGTITTLQSFHETFSMSLNDFVNNHHANRNLRVRSSWVKYVFNGVCLELERAAAWTGAPLLEDTECCGKERCQHATEESEYKKLVYVAVHLVNVLERLSLPSAHIDDSDEEKEPKADVRRLMIVCHELHIPLLHHTLEERIPDSNSAELGLPKAAVLEQLLELDLDVNATDKNNDTPMHILLRAREFRKSLVRALLVRGTWLFARNRHGDVVLNVMKRMKALNHANFDDLPLGRHITLAGLVANAMRVKYPKKFVGVEEQMPLELRRFYLAH
InterPro domains: - IPR036770: Ankyrin repeat-containing domain superfamily (homologous_superfamily) [4-611]
- IPR002110: Ankyrin repeat (repeat) [9-588]
- IPR011990: Tetratricopeptide-like helical domain superfamily (homologous_superfamily) [306-403]
Interaction partners: - Protein phosphatase fem-2
- Cullin-2
- Sex-determination protein fem-3
- Elongin-C
- RING-box protein 1
- RNA polymerase II transcription factor SIII p18 subunit
- Early embryogenesis protein zyg-11
- Leucine-Rich Repeat protein
- Zyg eleven-related protein 1
- Valine--tRNA ligase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0022414 (reproductive process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0003006 (developmental process involved in reproduction), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0009887 (animal organ morphogenesis), GO:1901564 (organonitrogen compound metabolic process), GO:0035112 (genitalia morphogenesis), GO:0044248 (cellular catabolic process), GO:0048731 (system development), GO:0019538 (protein metabolic process), GO:0048513 (animal organ development), GO:0048608 (reproductive structure development), GO:0007548 (sex differentiation), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090598 (male anatomical structure morphogenesis), GO:0009057 (macromolecule catabolic process), GO:0061458 (reproductive system development), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0044265 (cellular macromolecule catabolic process), GO:0030163 (protein catabolic process), GO:0090596 (sensory organ morphogenesis), GO:0045138 (nematode male tail tip morphogenesis), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0010498 (proteasomal protein catabolic process), GO:0051603 (proteolysis involved in protein catabolic process), GO:0019941 (modification-dependent protein catabolic process), GO:0043161 (proteasome-mediated ubiquitin-dependent protein catabolic process), GO:0006511 (ubiquitin-dependent protein catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)