Protein: RQC trigger complex helicase SLH1 (EC 3.6.4.13) (Antiviral helicase SLH1) (SKI2-like helicase 1) (P53327)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Involved in activation of the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation. Drives the splitting of stalled ribosomes that are polyubiquitinated in a HEL2-dependent manner, as part of the ribosome quality control trigger (RQT) complex. Also represses the translation of non-poly(A) mRNAs together with SKI2. May block translation by inhibiting translation initiation factor 5B (FUN12) action on mRNAs lacking a 3' poly(A) structure. Involved in antiviral defense, preventing L-A dsRNA virus propagation by specifically blocking translation of viral mRNAs.
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:14562095}.
Sequence: MSTEYSADSSKSFMIAMQSMIDTSQTFNLDRSKISLPDFDDELKKVQKDEQNQRTELTVLSQDRNDWDDIFEEFKDISFAQLQSIIDSYKTKNAVAVYKKIGKLINEAETTLSSNVLLETVLQMVYKHQKQELEKELLDFLGTGNIDLVSLLLQHRRMIVATPIETTILLIKNAVNSTPEFLTQQDIRNQVLKSAEDAKNRKLNPATKIIKYPHVFRKYEAGSTTAMAFAGQKFTLPVGTTRMSYNTHEEIIIPAADQASNKNYLYTKLLKISDLDHFCKTVFPYETLNQIQSLVYPVAYKTNENMLICAPTGAGKTDIALLTIINTIKQFSVVNGENEIDIQYDDFKVIYVAPLKALAAEIVDKFSKKLAPFNIQVRELTGDMQLTKAEILATQVIVTTPEKWDVVTRKANGDNDLVSKVKLLIIDEVHLLHEDRGSVIETLVARTLRQVESSQSMIRIIGLSATLPNFMDVADFLGVNRQIGMFYFDQSFRPKPLEQQLLGCRGKAGSRQSKENIDKVAYDKLSEMIQRGYQVMVFVHSRKETVKSARNFIKLAESNHEVDLFAPDPIEKDKYSRSLVKNRDKDMKEIFQFGFGIHHAGMARSDRNLTEKMFKDGAIKVLCCTATLAWGVNLPADCVIIKGTQVYDSKKGGFIDLGISDVIQIFGRGGRPGFGSANGTGILCTSNDRLDHYVSLITQQHPIESRFGSKLVDNLNAEISLGSVTNVDEAIEWLGYTYMFVRMRKNPFTYGIDWEEIANDPQLYERRRKMIVVAARRLHALQMIVFDEVSMHFIAKDLGRVSSDFYLLNESVEIFNQMCDPRATEADVLSMISMSSEFDGIKFREEESKELKRLSDESVECQIGSQLDTPQGKANVLLQAYISQTRIFDSALSSDSNYVAQNSVRICRALFLIGVNRRWGKFSNVMLNICKSIEKRLWAFDHPLCQFDLPENIIRRIRDTKPSMEHLLELEADELGELVHNKKAGSRLYKILSRFPKINIEAEIFPITTNVMRIHIALGPDFVWDSRIHGDAQFFWVFVEESDKSQILHFEKFILNRRQLNNQHEMDFMIPLSDPLPPQVVVKVVSDTWIGCESTHAISFQHLIRPFNETLQTKLLKLRPLPTSALQNPLIESIYPFKYFNPMQTMTFYTLYNTNENAFVGSPTGSGKTIVAELAIWHAFKTFPGKKIVYIAPMKALVRERVDDWRKKITPVTGDKVVELTGDSLPDPKDVHDATIVITTPEKFDGISRNWQTRKFVQDVSLIIMDEIHLLASDRGPILEMIVSRMNYISSQTKQPVRLLGMSTAVSNAYDMAGWLGVKDHGLYNFPSSVRPVPLKMYIDGFPDNLAFCPLMKTMNKPVFMAIKQHSPDKPALIFVASRRQTRLTALDLIHLCGMEDNPRRFLNIDDEEELQYYLSQVTDDTLKLSLQFGIGLHHAGLVQKDRSISHQLFQKNKIQILIATSTLAWGVNLPAHLVIIKGTQFFDAKIEGYRDMDLTDILQMMGRAGRPAYDTTGTAIVYTKESKKMFYKHFLNVGFPVESSLHKVLDDHLGAEITSGSITNKQEALDFLSWTFLFRRAHHNPTYYGIEDDTSTAGVSEHLSSLIDSTLENLRESQCVLLHGDDIVATPFLSISSYYYISHLTIRQLLKQIHDHATFQEVLRWLSLAVEYNELPVRGGEIIMNEEMSQQSRYSVESTFTDEFELPMWDPHVKTFLLLQAHLSRVDLPIADYIQDTVSVLDQSLRILQAYIDVASELGYFHTVLTMIKMMQCIKQGYWYEDDPVSVLPGLQLRRIKDYTFSEQGFIEMTPQQKKKKLLTLEEIGRFGYKKLLNVFDQLTFGMTESEDTKKRFVSVCQRLPVLEGMKFEEQENNEVLTFYSKHLSSKHNNGFEVYCDKFPKIQKELWFLIGHKGDELLMIKRCQPKQMNKEVIIHCDLFIPEEIRGEELQFSLINDALGLRYDMVHKLIS
InterPro domains: - IPR035892: C2 domain superfamily (homologous_superfamily) [995-1103]
- IPR036390: Winged helix DNA-binding domain superfamily (homologous_superfamily) [702-1638]
- IPR003593: AAA+ ATPase domain (domain) [302-1388]
- IPR057842: SNRNP200 helicase-like, winged helix-turn-helix domain (domain) [704-1619]
- IPR004179: Sec63 domain (domain) [795-1965]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [224-1538]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [284-1336]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [289-1307]
- IPR050474: Helicase Hel308/SKI2-like (family) [184-1108]
- IPR001650: Helicase, C-terminal domain-like (domain) [516-1550]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [704-1634]
Interaction partners: - RQC trigger complex subunit CUE3
- RQC trigger complex subunit RQT4
- E3 ubiquitin-protein ligase HEL2
- Pre-mRNA-splicing factor 8
- Pre-mRNA-splicing factor 6
- Pre-mRNA-splicing factor CEF1
- Pre-mRNA-processing factor 17
- Pre-mRNA-processing protein 45
- Pre-mRNA-processing factor 19
- Pre-mRNA-splicing factor SYF1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0004386 (helicase activity), GO:0008094 (ATP-dependent activity, acting on DNA), GO:0005515 (protein binding), GO:0140097 (catalytic activity, acting on DNA), GO:0003678 (DNA helicase activity), GO:0043138 (3'-5' DNA helicase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0043933 (protein-containing complex organization), GO:0010467 (gene expression), GO:0022607 (cellular component assembly), GO:0016070 (RNA metabolic process), GO:0022618 (ribonucleoprotein complex assembly), GO:0065003 (protein-containing complex assembly), GO:0006396 (RNA processing), GO:0071826 (ribonucleoprotein complex subunit organization), GO:0008380 (RNA splicing), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0000393 (spliceosomal conformational changes to generate catalytic conformation), GO:0000398 (mRNA splicing, via spliceosome), GO:0000375 (RNA splicing, via transesterification reactions), GO:0000377 (RNA splicing, via transesterification reactions with bulged adenosine as nucleophile)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:1990904 (ribonucleoprotein complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140513 (nuclear protein-containing complex), GO:0120114 (Sm-like protein family complex), GO:0030532 (small nuclear ribonucleoprotein complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0097525 (spliceosomal snRNP complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0097526 (spliceosomal tri-snRNP complex), GO:0005682 (U5 snRNP), GO:0005634 (nucleus), GO:0046540 (U4/U6 x U5 tri-snRNP complex)