Protein: tRNA(Thr) (cytosine(32)-N(3))-methyltransferase (EC 2.1.1.268) (Actin-binding protein of 140 kDa) (tRNA methyltransferase of 140 kDa) (Q08641)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: S-adenosyl-L-methionine-dependent methyltransferase that mediates N(3)-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA(Thr) and tRNA(Ser). N(3)-methylcytidine methylation of tRNA(Thr) requires the N6-threonylcarbamoylation of tRNA (t6A37) by the EKC/KEOPS complex as prerequisite. N(3)-methylcytidine methylation of tRNA(Ser) requires the formation of N(6)-dimethylallyladenosine(37) (i6A37) by MOD5 as prerequisite. Methylation of tRNA(Ser) is also stimulated by SES1. Binds F-actin and shows weak F-actin cross-linking activity.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:9467951}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:9467951}. Note=Cytoplasmic and cortical cytoskeleton. {ECO:0000269|PubMed:9467951}.
Sequence: MGVADLIKKFESISKEEGDATVDTNSSSKPLKSNDETKELHQQESTAVPQEVDVNEEFENEPETINSSRTAEKPLETNLPKPETNEEDEEEGSMSENKIYSKGENADINVNDFQEYKEMENTGAEVLASSVEESDAIQEGVAEETEGIATPKQKENEKNDESEEESANNASEPAEEYSQSEEDADIEQSNGKETENAENASQQANDGSTSTTTSKNKKKKNKKKNKKKRNGNVNTNANVDDSTKTGENDDTTGDTTSSTTSAIQEVNDLEVVDDSCLGIDQQHNREHLKALTQDVKEETLENIAHEGRGDNTGDQNAVEKSDFEKSDTEGSRIGRDLPFEFGKRNLTEESDVWDHNAWDNVEWGEEQVQQAEEKIKEQFKHPVPEFDKKLYNENPARYWDIFYKNNKENFFKDRKWLQIEFPILYASTRKDAEPVTIFEIGCGAGNTFFPILKDNENENLRIIAADFAPRAVELVKNSEQFNPKYGHATVWDLANPDGNLPDGVEPHSVDIAVMIFVFSALAPNQWDQAMDNLHKILKPGGKIIFRDYGAYDLTQVRFKKNRILEENFYVRGDGTRVYFFSEEKLREIFTKKYFLENKIGTDRRLLVNRKRQLKMYRCWVQAVFDVPQ
InterPro domains: - IPR026113: tRNA N(3)-methylcytidine methyltransferase METTL2/6/8-like (family) [355-627]
- IPR029063: S-adenosyl-L-methionine-dependent methyltransferase superfamily (homologous_superfamily) [364-624]
- IPR013217: Methyltransferase type 12 (domain) [439-543]
Interaction partners: - Actin
- Fimbrin
- tRNA
- Actin-binding protein
- BNI1-related protein 1
- Telomere replication protein EST3
- Tropomyosin-1
- Coronin-like protein
- Serine--tRNA ligase, cytoplasmic
- tRNA
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0016740 (transferase activity), GO:0140098 (catalytic activity, acting on RNA), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0008173 (RNA methyltransferase activity), GO:0008168 (methyltransferase activity), GO:0140101 (catalytic activity, acting on a tRNA), GO:0008175 (tRNA methyltransferase activity), GO:0008757 (S-adenosylmethionine-dependent methyltransferase activity), GO:0016427 (tRNA (cytosine) methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0032259 (methylation), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0043414 (macromolecule methylation), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0001510 (RNA methylation), GO:0010467 (gene expression), GO:0030488 (tRNA methylation), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0009451 (RNA modification), GO:0034470 (ncRNA processing), GO:0006400 (tRNA modification), GO:0034660 (ncRNA metabolic process), GO:0006399 (tRNA metabolic process), GO:0008033 (tRNA processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)