Protein: Spindle assembly checkpoint component MAD3 (Mitotic MAD3 protein) (P47074)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Component of the mitotic checkpoint complex (MCC) which inhibits the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20.
Subcellular location: Nucleus.
Sequence: MKAYAKKRISYMPSSPSQNVINFEEIETQKENILPLKEGRSAAALSKAIHQPLVEINQVKSSFEQRLIDELPALSDPITLYLEYIKWLNNAYPQGGNSKQSGMLTLLERCLSHLKDLERYRNDVRFLKIWFWYIELFTRNSFMESRDIFMYMLRNGIGSELASFYEEFTNLLIQKEKFQYAVKILQLGIKNKARPNKVLEDRLNHLLRELGENNIQLGNEISMDSLESTVLGKTRSEFVNRLELANQNGTSSDVNLTKNNVFVDGEESDVELFETPNRGVYRDGWENFDLKAERNKENNLRISLLEANTNLGELKQHEMLSQKKRPYDEKLPIFRDSIGRSDPVYQMINTKDQKPEKIDCNFKLIYCEDEESKGGRLEFSLEEVLAISRNVYKRVRTNRKHPREANLGQEESANQKEAEAQSKRPKISRKALVSKSLTPSNQGRMFSGEEYINCPMTPKGRSTETSDIISAVKPRQLTPILEMRESNSFSQSKNSEIISDDDKSSSSFISYPPQR
InterPro domains: - IPR012572: Mad3/Bub1 homology region 2 (domain) [330-398]
- IPR013212: Mad3/Bub1 homology region 1 (domain) [56-228]
- IPR015661: Mitotic spindle checkpoint protein Bub1/Mad3 (family) [25-436]
Interaction partners: - Mitotic spindle checkpoint component MAD2
- APC/C activator protein CDC20
- Spindle assembly checkpoint protein BUB3
- Serine/threonine-protein kinase MPS1
- Spindle assembly checkpoint component MAD1
- Anaphase-promoting complex subunit CDC23
- Anaphase-promoting complex subunit 1
- Anaphase-promoting complex subunit CDC27
- Anaphase-promoting complex subunit DOC1
- Anaphase-promoting complex subunit CDC16
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0044877 (protein-containing complex binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0048523 (negative regulation of cellular process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1903047 (mitotic cell cycle process), GO:0051129 (negative regulation of cellular component organization), GO:0035556 (intracellular signal transduction), GO:0019538 (protein metabolic process), GO:0010948 (negative regulation of cell cycle process), GO:0043170 (macromolecule metabolic process), GO:0006793 (phosphorus metabolic process), GO:0051726 (regulation of cell cycle), GO:0045786 (negative regulation of cell cycle), GO:0016043 (cellular component organization), GO:0051128 (regulation of cellular component organization), GO:0010564 (regulation of cell cycle process), GO:0000278 (mitotic cell cycle), GO:0007346 (regulation of mitotic cell cycle), GO:0007088 (regulation of mitotic nuclear division), GO:0000075 (cell cycle checkpoint signaling), GO:0045839 (negative regulation of mitotic nuclear division), GO:0006796 (phosphate-containing compound metabolic process), GO:1905818 (regulation of chromosome separation), GO:0007093 (mitotic cell cycle checkpoint signaling), GO:0036211 (protein modification process), GO:0010639 (negative regulation of organelle organization), GO:0051983 (regulation of chromosome segregation), GO:0043412 (macromolecule modification), GO:1901987 (regulation of cell cycle phase transition), GO:0045930 (negative regulation of mitotic cell cycle), GO:1905819 (negative regulation of chromosome separation), GO:0033043 (regulation of organelle organization), GO:0051985 (negative regulation of chromosome segregation), GO:0006996 (organelle organization), GO:1901988 (negative regulation of cell cycle phase transition), GO:0051783 (regulation of nuclear division), GO:0051784 (negative regulation of nuclear division), GO:0010965 (regulation of mitotic sister chromatid separation), GO:0033045 (regulation of sister chromatid segregation), GO:1901991 (negative regulation of mitotic cell cycle phase transition), GO:2000816 (negative regulation of mitotic sister chromatid separation), GO:0033046 (negative regulation of sister chromatid segregation), GO:0006468 (protein phosphorylation), GO:0016310 (phosphorylation), GO:1902099 (regulation of metaphase/anaphase transition of cell cycle), GO:0033048 (negative regulation of mitotic sister chromatid segregation), GO:0031577 (spindle checkpoint signaling), GO:0045841 (negative regulation of mitotic metaphase/anaphase transition), GO:0033047 (regulation of mitotic sister chromatid segregation), GO:1901990 (regulation of mitotic cell cycle phase transition), GO:1902100 (negative regulation of metaphase/anaphase transition of cell cycle), GO:2001251 (negative regulation of chromosome organization), GO:0033044 (regulation of chromosome organization), GO:0071174 (mitotic spindle checkpoint signaling), GO:0007094 (mitotic spindle assembly checkpoint signaling), GO:0071173 (spindle assembly checkpoint signaling), GO:0030071 (regulation of mitotic metaphase/anaphase transition)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098687 (chromosomal region), GO:0099080 (supramolecular complex), GO:0140513 (nuclear protein-containing complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000775 (chromosome, centromeric region), GO:0000776 (kinetochore), GO:0043228 (non-membrane-bounded organelle), GO:0000779 (condensed chromosome, centromeric region), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome), GO:0005634 (nucleus), GO:0000793 (condensed chromosome)