Protein: Polyadenylate-binding protein 2 (PABP-2) (Poly(A)-binding protein 2) (dPABP2) (Nuclear poly(A)-binding protein 1) (Poly(A)-binding protein II) (PABII) (Polyadenylate-binding nuclear protein 1) (Protein rox2) (Q7KNF2)
Organism: Drosophila melanogaster (Fruit fly)
Function: Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (By similarity). Increases the affinity of poly(A) polymerase for RNA (By similarity). Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation (By similarity). Plays a role in the positive regulation of alpha-1,3 fucosylation, possibly by cooperating with swm which regulates nuclear export of fucosyltransferase FucTA.
Subcellular location: Nucleus {ECO:0000269|PubMed:10481015}. Cytoplasm {ECO:0000269|PubMed:10481015}. Note=Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus. Mostly found in the nucleus in the germarium. From egg chamber formation onwards, expressed in both the nuclei and cytoplasm of nurse cells and the oocyte. At stage 10, abundant in the nuclei of nurse cells, oocyte and follicle cells and in the cytoplasm of nurse cells. Uniformly distributed in the cytoplasm of early embryos then progressively localizes to nuclei during the pre-blastoderm and syncytial blastoderm stages. After cellularization, localizes mainly to the nucleus and remains nuclear in later embryonic and larval stages. During mitosis, expression levels are reduced in prophase nuclei and expression is localized to the cytoplasm during metaphase. Localizes to nuclear speckles.
Sequence: MADEDITLNEDQLLESLEETNGEQETEIATEVEEEGSMQIDPELEAIKARVKEMEEEAEKIKQMQSEVDKQMAGGSTTGLATVPLSLEEKQEIDTRSVYVGNVDYGASAEELEAHFHGCGTINRVTILCNKADGHPKGFAYIEFGSKEFVETALAMNETLFRGRQIKVMSKRTNRPGLSTTNRFARGSFRGRGARVSRACCHSTFRGARRAMGYRGRANYYAPY
InterPro domains: - IPR035979: RNA-binding domain superfamily (homologous_superfamily) [83-195]
- IPR012677: Nucleotide-binding alpha-beta plait domain superfamily (homologous_superfamily) [10-179]
- IPR000504: RNA recognition motif domain (domain) [96-173]
Interaction partners: - polynucleotide adenylyltransferase
- Cleavage stimulation factor 64 kD subunit
- Polyadenylate-binding protein
- Nuclear cap-binding protein subunit 2
- LD40489p
- Nuclear cap-binding protein subunit 1
- Dosage compensation regulator mle
- Zinc finger CCCH domain-containing protein 3
- Splicing factor U2AF 50 kDa subunit
- LD29830p
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0003727 (single-stranded RNA binding), GO:0003729 (mRNA binding), GO:0070717 (poly-purine tract binding), GO:0003730 (mRNA 3'-UTR binding), GO:0008143 (poly(A) binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0009605 (response to external stimulus), GO:0044237 (cellular metabolic process), GO:0009607 (response to biotic stimulus), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0007049 (cell cycle), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0051707 (response to other organism), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0007059 (chromosome segregation), GO:1901360 (organic cyclic compound metabolic process), GO:1903047 (mitotic cell cycle process), GO:0043207 (response to external biotic stimulus), GO:0035556 (intracellular signal transduction), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0009617 (response to bacterium), GO:0044770 (cell cycle phase transition), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0000278 (mitotic cell cycle), GO:0044772 (mitotic cell cycle phase transition), GO:0051225 (spindle assembly), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0042770 (signal transduction in response to DNA damage), GO:0042742 (defense response to bacterium), GO:0072359 (circulatory system development), GO:0000165 (MAPK cascade), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0000082 (G1/S transition of mitotic cell cycle), GO:0006396 (RNA processing), GO:0009451 (RNA modification), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0031123 (RNA 3'-end processing), GO:0043631 (RNA polyadenylation), GO:0006378 (mRNA polyadenylation), GO:0031124 (mRNA 3'-end processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:1990904 (ribonucleoprotein complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000785 (chromatin), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0016234 (inclusion body), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0042405 (nuclear inclusion body), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005694 (chromosome), GO:0005634 (nucleus)