Protein: Calcium-binding protein 1 (P38505)
Organism: Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM)
Function: Calcium-binding protein which probably acts as a calcium sensor and modulator, contributing to cellular Ca(2+)-mediated signaling. Activates endogenous kinase(s) in a Ca(2+)-dependent manner. Plays a role in regulating actin cytoskeleton dynamics. Regulates the association of actin filaments in a bundle but not actin polymerization and/or nucleation processes. Required for host cell phagocytosis and macropinocytosis
Subcellular location: Cytoplasm. Cell projection, phagocytic cup. Cell projection, pseudopodium. Note=During host erythrocyte phagocytosis, co-localizes with F-actin to the phagocytic cup. Leaves the phagocytic cup before the formation of phagosomes. Co-localizes with FAK in the pseudopodium
Sequence: MAEALFKEIDVNGDGAVSYEEVKAFVSKKRAIKNEQLLQLIFKSIDADGNGEIDQNEFAKFYGSIQGQDLSDDKIGLKVLYKLMDVDGDGKLTKEEVTSFFKKHGIEKVAEQVMKADANGDGYITLEEFLEFSL
InterPro domains: - IPR002048: EF-hand domain (domain) [1-134]
- IPR039647: EF-hand domain pair protein CML-like (family) [3-60]
- IPR011992: EF-hand domain pair (homologous_superfamily) [4-131]
- IPR018247: EF-Hand 1, calcium-binding site (binding_site) [10-129]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0043167 (ion binding), GO:0043169 (cation binding), GO:0046872 (metal ion binding), GO:0005509 (calcium ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0065008 (regulation of biological quality), GO:0050794 (regulation of cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0048523 (negative regulation of cellular process), GO:0051129 (negative regulation of cellular component organization), GO:0016043 (cellular component organization), GO:0051128 (regulation of cellular component organization), GO:0032970 (regulation of actin filament-based process), GO:0090066 (regulation of anatomical structure size), GO:0044087 (regulation of cellular component biogenesis), GO:0031333 (negative regulation of protein-containing complex assembly), GO:0032535 (regulation of cellular component size), GO:1902903 (regulation of supramolecular fiber organization), GO:0010639 (negative regulation of organelle organization), GO:0043254 (regulation of protein-containing complex assembly), GO:0043244 (regulation of protein-containing complex disassembly), GO:0033043 (regulation of organelle organization), GO:0032956 (regulation of actin cytoskeleton organization), GO:1902904 (negative regulation of supramolecular fiber organization), GO:0032272 (negative regulation of protein polymerization), GO:0030835 (negative regulation of actin filament depolymerization), GO:0051493 (regulation of cytoskeleton organization), GO:0051494 (negative regulation of cytoskeleton organization), GO:1901879 (regulation of protein depolymerization), GO:0032271 (regulation of protein polymerization), GO:0030837 (negative regulation of actin filament polymerization), GO:0110053 (regulation of actin filament organization), GO:0030832 (regulation of actin filament length), GO:0008064 (regulation of actin polymerization or depolymerization), GO:0030833 (regulation of actin filament polymerization), GO:0051693 (actin filament capping), GO:0030834 (regulation of actin filament depolymerization), GO:0051016 (barbed-end actin filament capping)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)