Protein: Thiamine thiazole synthase (Q5ANB7)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5- (2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron- dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance
Subcellular location: Cytoplasm. Nucleus
Sequence: MTPPTMLQTAPVESFNLNPKSTQQAINLKSDAKNGKVSFADWNEFKFAPIRESTVSRAMTRRYFADLDKFAESDIVIIGAGSAGLSAAYTLGKNRPDLKIAIIEASVSPGGGCWLGGQLFSAMVLRKPAHLFLDDMGLDYEDEGDYVVVKHAALFMSTLMSKVLQFPNIKLFNATAVEDLITRKDPATNLQRIAGVVVNWAQLDHDTQSCMDPNTINCNVVLSTSGHDGPFGAFTAKRLEQLGRAPRDVTAGFTKPSITTSKLQEPEPISNFQLGGMKGLDMNKAEDAIVKGTREVVPGLVIAGMELAEVDGSNRMGPTFGAMALSGVKAAESVLNVLELRKQQNEACYGAYKG
InterPro domains: - IPR036188: FAD/NAD(P)-binding domain superfamily (homologous_superfamily) [40-348]
- IPR027495: Thiamine thiazole synthase (family) [2-347]
- IPR002922: Thiazole biosynthetic enzyme Thi4 family (family) [52-242]
Interaction partners: - 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase
- Trifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase/thiaminase
- Bifunctional hydroxyethylthiazole kinase/thiamine-phosphate diphosphorylase
- Pdc2p
- Prefoldin subunit 3
- Uncharacterized protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0043167 (ion binding), GO:0043169 (cation binding), GO:0046872 (metal ion binding), GO:0046914 (transition metal ion binding), GO:0005506 (iron ion binding), GO:0008198 (ferrous iron binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0016043 (cellular component organization), GO:0006066 (alcohol metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006766 (vitamin metabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006767 (water-soluble vitamin metabolic process), GO:0009110 (vitamin biosynthetic process), GO:0044272 (sulfur compound biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0034308 (primary alcohol metabolic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0042723 (thiamine-containing compound metabolic process), GO:0006996 (organelle organization), GO:0072527 (pyrimidine-containing compound metabolic process), GO:0046165 (alcohol biosynthetic process), GO:0042364 (water-soluble vitamin biosynthetic process), GO:0007005 (mitochondrion organization), GO:0006772 (thiamine metabolic process), GO:0034309 (primary alcohol biosynthetic process), GO:0042724 (thiamine-containing compound biosynthetic process), GO:0072528 (pyrimidine-containing compound biosynthetic process), GO:0009228 (thiamine biosynthetic process), GO:0000002 (mitochondrial genome maintenance)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0030312 (external encapsulating structure), GO:0031012 (extracellular matrix), GO:0062039 (biofilm matrix), GO:0062040 (fungal biofilm matrix)