Protein: SET domain-containing protein 4 (EC 2.1.1.-) (EC 2.1.1.364) (Q9NVD3)
Organism: Homo sapiens (Human)
Function: Histone-lysine N-methyltransferase that acts as a regulator of cell proliferation, cell differentiation and inflammatory response. Regulates the inflammatory response by mediating mono- and dimethylation of 'Lys-4' of histone H3 (H3K4me1 and H3K4me2, respectively), leading to activate the transcription of pro-inflammatory cytokines IL6 and TNF-alpha (By similarity). Also involved in the regulation of stem cell quiescence by catalyzing the trimethylation of 'Lys-20' of histone H4 (H4K20me3), thereby promoting heterochromatin formation. Involved in proliferation, migration, paracrine and myogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) (By similarity).
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:24738023, ECO:0000269|PubMed:37926288}. Nucleus {ECO:0000269|PubMed:24738023}.
Sequence: MQKGKGRTSRIRRRKLCGSSESRGVNESHKSEFIELRKWLKARKFQDSNLAPACFPGTGRGLMSQTSLQEGQMIISLPESCLLTTDTVIRSYLGAYITKWKPPPSPLLALCTFLVSEKHAGHRSLWKPYLEILPKAYTCPVCLEPEVVNLLPKSLKAKAEEQRAHVQEFFASSRDFFSSLQPLFAEAVDSIFSYSALLWAWCTVNTRAVYLRPRQRECLSAEPDTCALAPYLDLLNHSPHVQVKAAFNEETHSYEIRTTSRWRKHEEVFICYGPHDNQRLFLEYGFVSVHNPHACVYVSREILVKYLPSTDKQMDKKISILKDHGYIENLTFGWDGPSWRLLTALKLLCLEAEKFTCWKKVLLGEVISDTNEKTSLDIAQKICYYFIEETNAVLQKVSHMKDEKEALINQLTLVESLWTEELKILRASAETLHSLQTAFT
InterPro domains: - IPR036464: Rubisco LSMT, substrate-binding domain superfamily (homologous_superfamily) [303-439]
- IPR046341: SET domain superfamily (homologous_superfamily) [35-289]
- IPR016852: SET domain methyltransferase (family) [1-436]
- IPR044429: SETD4, SET domain (domain) [46-287]
- IPR001214: SET domain (domain) [48-273]
- IPR015353: Rubisco LSMT, substrate-binding domain (domain) [309-425]
- IPR050600: SETD3/SETD6 methyltransferase (family) [22-391]
Interaction partners: - N-lysine methyltransferase SETD6
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0008276 (protein methyltransferase activity), GO:0016279 (protein-lysine N-methyltransferase activity), GO:0008168 (methyltransferase activity), GO:0042054 (histone methyltransferase activity), GO:0018024 (histone lysine N-methyltransferase activity), GO:0140938 (histone H3 methyltransferase activity), GO:0008757 (S-adenosylmethionine-dependent methyltransferase activity), GO:0008170 (N-methyltransferase activity), GO:0016278 (lysine N-methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0032259 (methylation), GO:0019222 (regulation of metabolic process), GO:0048584 (positive regulation of response to stimulus), GO:0051240 (positive regulation of multicellular organismal process), GO:0006807 (nitrogen compound metabolic process), GO:0051239 (regulation of multicellular organismal process), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0009893 (positive regulation of metabolic process), GO:0044238 (primary metabolic process), GO:0032103 (positive regulation of response to external stimulus), GO:1901564 (organonitrogen compound metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0080134 (regulation of response to stress), GO:0019538 (protein metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0032101 (regulation of response to external stimulus), GO:0043414 (macromolecule methylation), GO:0001817 (regulation of cytokine production), GO:0043170 (macromolecule metabolic process), GO:0042127 (regulation of cell population proliferation), GO:0001819 (positive regulation of cytokine production), GO:0031349 (positive regulation of defense response), GO:0044260 (cellular macromolecule metabolic process), GO:1903555 (regulation of tumor necrosis factor superfamily cytokine production), GO:0031347 (regulation of defense response), GO:0006479 (protein methylation), GO:1903557 (positive regulation of tumor necrosis factor superfamily cytokine production), GO:0032755 (positive regulation of interleukin-6 production), GO:0032675 (regulation of interleukin-6 production), GO:0010628 (positive regulation of gene expression), GO:0010468 (regulation of gene expression), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0050727 (regulation of inflammatory response), GO:0050729 (positive regulation of inflammatory response), GO:0008213 (protein alkylation), GO:0018193 (peptidyl-amino acid modification), GO:0018022 (peptidyl-lysine methylation), GO:0032680 (regulation of tumor necrosis factor production), GO:0016571 (histone methylation), GO:0016570 (histone modification), GO:0032760 (positive regulation of tumor necrosis factor production), GO:0034968 (histone lysine methylation), GO:0018027 (peptidyl-lysine dimethylation), GO:0018205 (peptidyl-lysine modification), GO:0051568 (histone H3-K4 methylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)