Protein: Telomerase reverse transcriptase (AtTERT) (EC 2.7.7.49) (Q9SPU7)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Required to prevent genome instability induced by breakage-fusion-bridge (BFB) cycles. Can extend completely non-telomeric sequences using RNA template in vitro.
Subcellular location: Nucleus {ECO:0000269|PubMed:18212040}. Chromosome, telomere {ECO:0000250}.
Sequence: MPRKPRHRVPEILWRLFGNRARNLNDAIVDLIPNRNIQPEQCRCRGQGCLGCSSDKPAFLLRSDDPIHYRKLLHRCFVVLHEQTPPLLDFSPTSWWSQREIVERIIEMMQSGCDCQNVICARYDKYDQSSPILELLTSSSWEFLLKRVGHDVMVYLLQQTSIFLPLLGKKHQQVSGPPLCIKHKRTLSVHENKRKRDDNVQPPTKRQWLSSAVDDCPKDDSATITPIVGEDVDQHREKKTTKRSRIYLKRRRKQRKVNFKKVDCNAPCITPSTNGKVSTGNDEMNLHIGINGSLTDFVKQAKQVKRNKNFKFGLSETYSVIPPNHILKTLRPNCSDSKLLMNHIFGEVNVWSTTPSHGKGNCPSGSICLYHSLLKSLKNLIGKTKSSHLKMLLDKHCPVLLLQEDALKSGTTSQSSRRQKADKLPHGSSSSQTGKPKCPSVEERKLYCTNDQVVSFIWAICRYIVPESLLGTTHQMRVLRKNIAWFVSRRRNEKCTVNQFLHKVKPSDFPFFARKELCCMVNGHELQSESIRSTQQMLCTKWISWLFLEIVKKLVHFNFYATESQGGRLNIYYYRKRSWERLISKEISKALDGYVLVDDAEAESSRKKLSKFRFLPKANGVRMVLDFSSSSRSQSLRDTHAVLKDIQLKEPDVLGSSVFDHDDFYRNLCPYLIHLRSQSGELPPLYFVVADVFKAFDSVDQGKLLHVIQSFLKDEYILNRCRLVCCGKRSNWVNKILVSSDKNSNFSRFTSTVPYNALQSIVVDKGENHRVRKKDLMVWIGNMLKNNMLQLDKSFYVQIAGIPQGHRLSSLLCCFYYGHLERTLIYPFLEEASKDVSSKECSREEELIIPTSYKLLRFIDDYLFVSTSRDQASSFYHRLKHGFKDYNCFMNETKFCINFEDKEEHRCSSNRMFVGDNGVPFVRWTGLLINSRTFEVQVDYTRYLSGHISSTFSVAWQNKPVRNLRQKLCYFLVPKCHPILFDSNINSGEIVRLNIYQIFLLAAMKFHCYVYEVSRFWKLHPQTLFKFITISVRYMFRLINRRVRRINTGSSFRPVLKLYKEEVIWLGLDAYIQVLKKKNSRYRMLLIYLKSALSKHSLSQQLSSELRYATDRSNSSSLWKLNY
InterPro domains: - IPR049139: Telomerase reverse transcriptase, C-terminal extension (domain) [962-1089]
- IPR043502: DNA/RNA polymerase superfamily (homologous_superfamily) [693-910]
- IPR000477: Reverse transcriptase domain (domain) [596-929]
- IPR003545: Telomerase reverse transcriptase (family) [4-1123]
- IPR021891: Telomerase ribonucleoprotein complex - RNA-binding domain (domain) [451-592]
Interaction partners: - Protection of telomeres protein 1b
- H/ACA ribonucleoprotein complex subunit 4
- ATP-dependent DNA helicase 2 subunit KU70
- Protection of telomeres protein 1c
- H/ACA ribonucleoprotein complex subunit 3-like protein
- Protection of telomeres protein 1a
- ATP-dependent DNA helicase 2 subunit KU80
- Putative H/ACA ribonucleoprotein complex subunit 1-like protein 1
- Nonsense-mediated mRNA decay factor SMG7-like
- Nonsense-mediated mRNA decay factor SMG7
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0140097 (catalytic activity, acting on DNA), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0003723 (RNA binding), GO:0034061 (DNA polymerase activity), GO:0016779 (nucleotidyltransferase activity), GO:0070034 (telomerase RNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006259 (DNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0022616 (DNA strand elongation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)