Protein: Translation initiation factor IF-1, chloroplastic (O82499)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.
Subcellular location: Plastid, chloroplast {ECO:0000269|PubMed:11251102}.
Sequence: MLQLCSTFRPQLLLPCQFRFTNGVLIPQINYVASNSVVNIRPMIRCQRASGGRGGANRSKPAKPQVKEGSNKTVIEGLVTESLPNGMFRVDLENGDNILGYICGKIRKNFIRILPGDKVKVEMSVYDSTKGRIIFRMSSRD
InterPro domains: - IPR006196: RNA-binding domain, S1, IF1 type (domain) [75-138]
- IPR003029: S1 domain (domain) [70-138]
- IPR012340: Nucleic acid-binding, OB-fold (homologous_superfamily) [65-141]
- IPR004368: Translation initiation factor IF-1 (family) [48-140]
Interaction partners: - Small ribosomal subunit protein uS10c
- Translation initiation factor IF3-1, mitochondrial
- Small ribosomal subunit protein uS10z/uS10x
- Small ribosomal subunit protein uS14c
- Small ribosomal subunit protein uS10y
- Eukaryotic translation initiation factor 2 subunit alpha
- Eukaryotic translation initiation factor 2 subunit alpha homolog
- Small ribosomal subunit protein uS14z/uS14y/uS14x
- Small ribosomal subunit protein uS14m
- Eukaryotic translation initiation factor 3 subunit A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0003729 (mRNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0016043 (cellular component organization), GO:0044085 (cellular component biogenesis), GO:0043933 (protein-containing complex organization), GO:0006996 (organelle organization), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0065003 (protein-containing complex assembly), GO:0042254 (ribosome biogenesis), GO:0042274 (ribosomal small subunit biogenesis), GO:0022618 (ribonucleoprotein complex assembly), GO:0071826 (ribonucleoprotein complex subunit organization), GO:0000028 (ribosomal small subunit assembly), GO:0042255 (ribosome assembly), GO:0140694 (non-membrane-bounded organelle assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0009532 (plastid stroma), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0005737 (cytoplasm), GO:0031967 (organelle envelope), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009570 (chloroplast stroma), GO:0009526 (plastid envelope), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle), GO:0009941 (chloroplast envelope)