Protein: Erythronolide synthase EryA1 (EC 2.3.1.94) (6-deoxyerythronolide B synthase I) (6-deoxyerythronolide-B synthase EryA1, modules 1 and 2) (DEBS 1) (ORF C) (Q03131)
Organism: Saccharopolyspora erythraea (Streptomyces erythraeus)
Function: Involved in the biosynthesis of antibiotic erythromycin via the biosynthesis of its aglycone precursor, 6-deoxyerythronolide B (6-dEB).
Sequence: MSGPRSRTTSRRTPVRIGAVVVASSTSELLDGLAAVADGRPHASVVRGVARPSAPVVFVFPGQGAQWAGMAGELLGESRVFAAAMDACARAFEPVTDWTLAQVLDSPEQSRRVEVVQPALFAVQTSLAALWRSFGVTPDAVVGHSIGELAAAHVCGAAGAADAARAAALWSREMIPLVGNGDMAAVALSADEIEPRIARWDDDVVLAGVNGPRSVLLTGSPEPVARRVQELSAEGVRAQVINVSMAAHSAQVDDIAEGMRSALAWFAPGGSEVPFYASLTGGAVDTRELVADYWRRSFRLPVRFDEAIRSALEVGPGTFVEASPHPVLAAALQQTLDAEGSSAAVVPTLQRGQGGMRRFLLAAAQAFTGGVAVDWTAAYDDVGPNPALCRSSRRPRRKTSRPSPASTGTRHRTCCERLLAVVNGETAALAGREADAEATFRELGLDSVLAAQLRAKVSAAIGREVNIALLYDHPTPRALAEALAAGTEVAQRETRARTNEAAPGEPVAVVAMACRLPGGVSTPEEFWELLSEGRDAVAGLPTDRGWDLDSLFHPDPTRSGTAHQRGGGFLTEATAFDPAFFGMSPREALAVDPQQRLMLELSWEVLERAGIPPTSLQASPTGVFVGLIPQEYGPRLAEGGEGVEGYLMTGTTTSVASGRIAYTLGLEGPAISVDTACSSSLVAVHLACQSLRRGESSLAMAGGVTVMPTPGMLVDFSRMNSLAPDGRCKAFSAGANGFGMAEGAGMLLLERLSDARRNGHPVLAVLRGTAVNSDGASNGLSAPNGRAQVRVIQQALAESGLGPADIDAVEAHGTGTRLGDPIEARALFEAYGRDREQPLHLGSVKSNLGHTQAAAGVAGVIKMVLAMRAGTLPRTLHASERSKEIDWSSGAISLLDEPEPWPAGARPRRAGVSSFGISGTNAHAIIEEAPQVVEGERVEAGDVVAPWVLSASSAEGLRAQAARLAAHLREHPGQDPRDIAYSLATGRAALPHRAAFAPVDESAALRVLDGLATGNADGAAVGTSRAQQRAVFVFPGQGWQWAGMAVDLLDTSPVFAAALRECADALEPHLDFEVIPFLRAEAARREQDAALSTERVDVVQPVMFAVMVSLASMWRAHGVEPAAVIGHSQGEIAAACVAGALSLDDAARVVALRSRVIATMPGNKGMASIAAPAGEVRARIGDRVEIAAVNGPRSVVVAGDSDELDRLVASCTTECIRAKRLAVDYASHSSHVETIRDALHAELGEDFHPLPGFVPFFSTVTGRWTQPDELDAGYWYRNLRRTVRFADAVRALAEQGYRTFLEVSAHPILTAAIEEIGDGSGADLSAIHSLRRGDGSLADFGEALSRAFAAGVAVDWESVHLGTGARRVPLPTYPFQRERVWLEPKPVARRSTEVDEVSALRYRIEWRPTGAGEPARLDGTWLVAKYAGTADETSTAAREALESAGARVRELVVDARCGRDELAERLRSVGEVAGVLSLLAVDEAEPEEAPLALASLADTLSLVQAMVSAELGCPLWTVTESAVATGPFERVRNAAHGALWGVGRVIALENPAVWGGLVDVPAGSVAELARHLAAVVSGGAGEDQLALRADGVYGRRWVRAAAPATDDEWKPTGTVLVTGGTGGVGGQIARWLARRGAPHLLLVSRSGPDADGAGELVAELEALGARTTVAACDVTDRESVRELLGGIGDDVPLSAVFHAAATLDDGTVDTLTGERIERASRAKVLGARNLHELTRELDLTAFVLFSSFASAFGAPGLGGYAPGNAYLDGLAQQRRSDGLPATAVAWGTWAGSGMAEGAVADRFRRHGVIEMPPETACRALQNALDRAEVCPIVIDVRWDRFLLAYTAQRPTRLFDEIDDARRAAPQAPAEPRVGALASLPAPEREEALFELVRSHAAAVLGHASAERVPADQAFAELGVDSLSALELRNRLGAATGVRLPTTTVFDHPDVRTLAAHLAAELGGATGAEQAAPATTAPVDEPIAIVGMACRLPGEVDSPERLWELITSGRDSAAEVPDDRGWVPDELMASDAAGTRAHGNFMAGAGDFDAAFFGISPREALAMDPQQRQALETTWEALESAGIPPETLRGSDTGVFVGMSHQGYATGRPRPEDGVDGYLLTGNTASVASGRIAYVLGLEGPALTVDTACSSSLVALHTACGSLRDGDCGLAVAGGVSVMAGPEVFTEFSRQGALSPDGRCKPFSDEADGFGLGEGSAFVVLQRLSDARREGRRVLGVVAGSAVNQDGASNGLSAPSGVAQQRVIRRAWARAGITGADVAVVEAHGTGTRLGDPVEASALLATYGKSRGSSGPVLLGSVKSNIGHAQAAAGVAGVIKVLLGLERGVVPPMLCRGERSGLIDWSSGEIELADGVREWSPAADGVRRAGVSAFGVSGTNAHVIIAEPPEPEPVPQPRRMLPATGVVPVVLSARTGAALRAQAGRLADHLAAHPGIAPADVSWTMARARQHFEERAAVLAADTAEAVHRLRAVADGAVVPGVVTGSASDGGSVFVFPGQGAQWEGMARELLPVPVFAESIAECDAVLSEVAGFSVSEVLEPRPDAPSLERVDVVQPVLFAVMVSLARLWRACGAVPSAVIGHSQGEIAAAVVAGALSLEDGMRVVARRSRAVRAVAGRGSMLSVRGGRSDVEKLLADDSWTGRLEVAAVNGPDAVVVAGDAQAAREFLEYCEGVGIRARAIPVDYASHTAHVEPVRDELVQALAGITPRRAEVPFFSTLTGDFLDGTELDAGYWYRNLRHPVEFHSAVQALTDQGYATFIEVSPHPVLASSVQETLDDAESDAAVLGTLERDAGDADRFLTALADAHTRGVAVDWEAVLGRAGLVDLPGYPFQGKRFWLLPDRTTPRDELDGWFYRVDWTEVPRSEPAALRGRWLVVVPEGHEEDGWTVEVRSALAEAGAEPEVTRGVGGLVGDCAGVVSLLALEGDGAVQTLVLVRELDAEGIDAPLWTVTFGAVDAGSPVARPDQAKLWGLGQVASLERGPRWTGLVDLPHMPDPELRGRLTAVLAGSEDQVAVRADAVRARRLSPAHVTATSEYAVPGGTILVTGGTAGLGAEVARWLAGRGAEHLALVSRRGPDTEGVGDLTAELTRLGARVSVHACDVSSREPVRELVHGLIEQGDVVRGVVHAAGLPQQVAINDMDEAAFDEVVAAKAGGAVHLDELCSDAELFLLFSSGAGVWGSARQGAYAAGNAFLDAFARHRRGRGLPATSVAWGLWAAGGMTGDEEAVSFLRERGVRAMPVPRALAALDRVLASGETAVVVTDVDWPAFAESYTAARPRPLLDRIVTTAPSERAGEPETESLRDRLAGLPRAERTAELVRLVRTSTATVLGHDDPKAVRATTPFKELGFDSLAAVRLRNLLNAATGLRLPSTLVFDHPNASAVAGFLDAELGTEVRGEAPSALAGLDALEGALPEVPATEREELVQRLERMLAALRPVAQAADASGTGANPSGDDLGEAGVDELLEALGRELDGD
InterPro domains: - IPR020841: Polyketide synthase, beta-ketoacyl synthase domain (domain) [504-2405]
- IPR036291: NAD(P)-binding domain superfamily (homologous_superfamily) [1396-3313]
- IPR020806: Polyketide synthase-like, phosphopantetheine-binding domain (domain) [419-3407]
- IPR018201: Beta-ketoacyl synthase, active site (active_site) [668-2155]
- IPR016035: Acyl transferase/acyl hydrolase/lysophospholipase (homologous_superfamily) [56-2795]
- IPR014030: Beta-ketoacyl synthase-like, N-terminal (domain) [505-2226]
- IPR036736: ACP-like superfamily (homologous_superfamily) [408-3412]
- IPR057326: Ketoreductase domain (domain) [1613-3235]
- IPR014031: Beta-ketoacyl synthase, C-terminal (domain) [763-2349]
- IPR013968: Polyketide synthase-like, ketoreductase domain (domain) [1613-3233]
- IPR014043: Acyl transferase domain (domain) [57-2827]
- IPR050091: Polyketide and Nonribosomal Peptide Biosynthesis Enzymes (family) [492-1391]
- IPR009081: Phosphopantetheine binding ACP domain (domain) [412-3407]
- IPR032821: Polyketide synthase, C-terminal extension (domain) [883-994]
- IPR006162: Phosphopantetheine attachment site (ptm) [442-3377]
- IPR016036: Malonyl-CoA ACP transacylase, ACP-binding (homologous_superfamily) [179-2699]
- IPR001227: Acyl transferase domain superfamily (homologous_superfamily) [57-2811]
- IPR016039: Thiolase-like (homologous_superfamily) [495-2419]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0033218 (amide binding), GO:0016740 (transferase activity), GO:0072341 (modified amino acid binding), GO:0019842 (vitamin binding), GO:0016746 (acyltransferase activity), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0016999 (antibiotic metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:0017000 (antibiotic biosynthetic process), GO:0044249 (cellular biosynthetic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0033067 (macrolide metabolic process), GO:0030638 (polyketide metabolic process), GO:0033068 (macrolide biosynthetic process), GO:1901334 (lactone metabolic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0030639 (polyketide biosynthetic process), GO:1901336 (lactone biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0005622 (intracellular anatomical structure), GO:0005737 (cytoplasm)