Protein: Vacuolar-sorting protein SNF8 (ELL-associated protein of 30 kDa) (ESCRT-II complex subunit VPS22) (hVps22) (Q96H20)
Organism: Homo sapiens (Human)
Function: Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Required for the exosomal release of SDCBP, CD63 and syndecan.
Subcellular location: Cytoplasm. Endosome membrane. Nucleus {ECO:0000305}. Late endosome membrane. Note=Recruited to the endosome membrane to participate in vesicle formation.
Sequence: MHRRGVGAGAIAKKKLAEAKYKERGTVLAEDQLAQMSKQLDMFKTNLEEFASKHKQEIRKNPEFRVQFQDMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLEELHQQVLKGRGKFAQDVSQDDLIRAIKKLKALGTGFGIIPVGGTYLIQSVPAELNMDHTVVLQLAEKNGYVTVSEIKASLKWETERARQVLEHLLKEGLAWLDLQAPGEAHYWLPALFTDLYSQEITAEEAREALP
InterPro domains: - IPR040608: Snf8/Vps36 family (family) [6-225]
- IPR036390: Winged helix DNA-binding domain superfamily (homologous_superfamily) [25-241]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [92-258]
- IPR016689: ESCRT-2 complex, Snf8 (family) [1-253]
Interaction partners: - Vacuolar protein-sorting-associated protein 25
- Vacuolar protein-sorting-associated protein 36
- Charged multivesicular body protein 6
- Vacuolar protein sorting-associated protein 28 homolog
- Tumor susceptibility gene 101 protein
- Charged multivesicular body protein 2a
- Charged multivesicular body protein 3
- RNA polymerase II elongation factor ELL
- Vacuolar protein sorting-associated protein 37C
- Charged multivesicular body protein 4b
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0051641 (cellular localization), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0033036 (macromolecule localization), GO:0009892 (negative regulation of metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0061919 (process utilizing autophagic mechanism), GO:0048523 (negative regulation of cellular process), GO:0007154 (cell communication), GO:0044238 (primary metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044248 (cellular catabolic process), GO:0019538 (protein metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0051649 (establishment of localization in cell), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0045184 (establishment of protein localization), GO:0006914 (autophagy), GO:0009991 (response to extracellular stimulus), GO:0046907 (intracellular transport), GO:0042594 (response to starvation), GO:0033554 (cellular response to stress), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0006810 (transport), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0031668 (cellular response to extracellular stimulus), GO:0080090 (regulation of primary metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0071496 (cellular response to external stimulus), GO:0071705 (nitrogen compound transport), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0009267 (cellular response to starvation), GO:0072594 (establishment of protein localization to organelle), GO:0030163 (protein catabolic process), GO:0071702 (organic substance transport), GO:0010468 (regulation of gene expression), GO:0016236 (macroautophagy), GO:0009057 (macromolecule catabolic process), GO:0006886 (intracellular protein transport), GO:0044265 (cellular macromolecule catabolic process), GO:0015031 (protein transport), GO:0031667 (response to nutrient levels), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0008104 (protein localization), GO:0007034 (vacuolar transport), GO:0006996 (organelle organization), GO:0051253 (negative regulation of RNA metabolic process), GO:0031669 (cellular response to nutrient levels), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0033365 (protein localization to organelle), GO:0072666 (establishment of protein localization to vacuole), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0051603 (proteolysis involved in protein catabolic process), GO:0006605 (protein targeting), GO:0006623 (protein targeting to vacuole), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0015867 (ATP transport), GO:0006862 (nucleotide transport), GO:0046015 (regulation of transcription by glucose), GO:0045892 (negative regulation of DNA-templated transcription), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:0019941 (modification-dependent protein catabolic process), GO:0072665 (protein localization to vacuole), GO:0006511 (ubiquitin-dependent protein catabolic process), GO:1904669 (ATP export), GO:0051503 (adenine nucleotide transport), GO:0043162 (ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0098796 (membrane protein complex), GO:0005615 (extracellular space), GO:0140513 (nuclear protein-containing complex), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0005576 (extracellular region), GO:0031090 (organelle membrane), GO:0043229 (intracellular organelle), GO:0043230 (extracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043233 (organelle lumen), GO:0070062 (extracellular exosome), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0065010 (extracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0070013 (intracellular organelle lumen), GO:1903561 (extracellular vesicle), GO:0005634 (nucleus), GO:0031981 (nuclear lumen)