Protein: Beta-apo-4'-carotenal oxygenase (Q1K615)
Organism: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
Function: Beta-apo-4'-carotenal oxygenase involved in the last step of synthesis of neurosporaxanthin, a carboxylic apocarotenoid acting as an essential protective pigment and leading to orange pigmentation. Converts the aldehyde beta-apo-4'-carotenal into neurosporaxanthin. Neurosporaxanthin is synthesized from geranyl-geranyl pyrophosphate (GGPP) through several enzymatic activities. Phytoene synthase activity performed by the bifunctional enzyme al-2 first produces phytoene from geranyl-geranyl pyrophosphate (GGPP). The phytoene dehydrogenase al-1 then introduces 5 desaturations to lead to 3,4-didehydrolycopene via the intermediates phytofluene, zeta-carotene, neurosporene and lycopene. Al-2 cyclase activity then converts 3,4-didehydrolycopene into torulene. Al-2 can also convet lycopene into gamma-carotene which in turn is converted to beta- carotene by an additional al-2 cyclization reaction. Torulene is the substrate of the dioxidase cao-2 that breaks the molecule, removing five carbon atoms to yield beta-apo-4'-carotenal, whereas the aldehyde dehydrogenase ylo-1 mediates the last step by converting beta-apo-4'- carotenal into neurosporaxanthin (Probable)
Sequence: MAASKVEIAPFEVTPLDAIPAVCSTARATFASHKTKNLQWRLVQLRKLYWALDDFKASLMAALQQDLRKGGYESDFTEVDWVKNDCLHMINNLETFAKTEKLKDLPVTYSMMNFRVKKEPLGTVLIIGPYNFPIQLVLAPLVGAIGAGCTAVIKPSELTPACAMAMKEMIESRLDRDAFAVVNGGVPETNALMEEKWDKIMFTGSAQVGSIIARKAAETLTPVCLELGGRNPAFVTKKANLALAARRLMWGKVLNAGQVCMSHNYVLVDKDVADTFIEFLKIAYKDMFPNGAKASPDLSRIVNARHFNRIKKMLDETKGKIVMGGEMDESELYIEPTAVLVDSLDDPMMQEESFGPIFSIYPVDTLDQALSIANNVHRTPLALMAFGDKSETNRILDEMTSGGACINDSYFHGAVHTVPFGGVGDSGWGAYRGKASFDNFTHFRTVSETPTWMDRFLRVRYMPYDWSELRLLQRWTNKKPNFDRQGTVAKGSEYWMWYFLGLGTKGGVKGALMRWLVVVAGYYLSAYMKARRA
InterPro domains: - IPR012394: Aldehyde dehydrogenase NAD(P)-dependent (family) [17-486]
- IPR016162: Aldehyde dehydrogenase, N-terminal (homologous_superfamily) [27-444]
- IPR016161: Aldehyde/histidinol dehydrogenase (homologous_superfamily) [21-464]
- IPR016163: Aldehyde dehydrogenase, C-terminal (homologous_superfamily) [229-426]
- IPR015590: Aldehyde dehydrogenase domain (domain) [17-446]
Interaction partners: - Carotenoid dioxygenase
- Peroxisomal membrane protein
- ATPase family AAA domain-containing protein 1
- Peroxin
- Hsc70 cochaperone
- Mitochondrial fission 1 protein
- Bifunctional lycopene cyclase/phytoene synthase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016903 (oxidoreductase activity, acting on the aldehyde or oxo group of donors), GO:0016620 (oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor), GO:0004030 (aldehyde dehydrogenase [NAD(P)+] activity), GO:0004029 (aldehyde dehydrogenase (NAD+) activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:0006066 (alcohol metabolic process), GO:0006629 (lipid metabolic process), GO:0034308 (primary alcohol metabolic process), GO:0006720 (isoprenoid metabolic process), GO:1901568 (fatty acid derivative metabolic process), GO:0006721 (terpenoid metabolic process), GO:0016101 (diterpenoid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0031090 (organelle membrane), GO:0031967 (organelle envelope), GO:0005739 (mitochondrion), GO:0043229 (intracellular organelle), GO:0019867 (outer membrane), GO:0043227 (membrane-bounded organelle), GO:0005740 (mitochondrial envelope), GO:0031968 (organelle outer membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0031966 (mitochondrial membrane), GO:0005741 (mitochondrial outer membrane)