Protein: Serine/threonine-protein kinase Nek7 (EC 2.7.11.34) (Never in mitosis A-related kinase 7) (NimA-related protein kinase 7) (Q9ES74)
Organism: Mus musculus (Mouse)
Function: Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (By similarity). Phosphorylates RPS6KB1 (By similarity). Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity. Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3:PYCARD complex, and activation of CASP1. Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division: interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis.
Subcellular location: Nucleus {ECO:0000269|PubMed:26188091}. Cytoplasm {ECO:0000269|PubMed:26188091}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000250|UniProtKB:Q8TDX7}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:Q8TDX7}. Note=Present at centrosome throughout the cell cycle (By similarity). Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (By similarity). Interaction with ANKS3 prevents its translocation to the nucleus (PubMed:26188091). {ECO:0000250|UniProtKB:Q8TDX7, ECO:0000269|PubMed:26188091}.
Sequence: MDEQSQGMQGPPVTQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRASCLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALDHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNICINPDPEKRPDIAYVYDVAKRMHACTAST
InterPro domains: - IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [17-290]
- IPR008271: Serine/threonine-protein kinase, active site (active_site) [157-169]
- IPR001245: Serine-threonine/tyrosine-protein kinase, catalytic domain (domain) [111-285]
- IPR017441: Protein kinase, ATP binding site (binding_site) [40-63]
- IPR000719: Protein kinase domain (domain) [34-299]
Interaction partners: - NACHT, LRR and PYD domains-containing protein 3
- Serine/threonine-protein kinase Nek9
- Caspase-1
- Ankyrin repeat and SAM domain-containing protein 3
- Gasdermin-D
- Ankyrin repeat and SAM domain-containing protein 6
- Apoptosis-associated speck-like protein containing a CARD
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0019222 (regulation of metabolic process), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0033036 (macromolecule localization), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0065009 (regulation of molecular function), GO:0007017 (microtubule-based process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0050790 (regulation of catalytic activity), GO:0044093 (positive regulation of molecular function), GO:0044085 (cellular component biogenesis), GO:1901564 (organonitrogen compound metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0007051 (spindle organization), GO:0019538 (protein metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0051649 (establishment of localization in cell), GO:0000226 (microtubule cytoskeleton organization), GO:0043170 (macromolecule metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:0045184 (establishment of protein localization), GO:0006793 (phosphorus metabolic process), GO:0051726 (regulation of cell cycle), GO:0007059 (chromosome segregation), GO:0031325 (positive regulation of cellular metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0051128 (regulation of cellular component organization), GO:0007346 (regulation of mitotic cell cycle), GO:0007088 (regulation of mitotic nuclear division), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051052 (regulation of DNA metabolic process), GO:0051054 (positive regulation of DNA metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0022607 (cellular component assembly), GO:0036211 (protein modification process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051225 (spindle assembly), GO:0043412 (macromolecule modification), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0033043 (regulation of organelle organization), GO:0008104 (protein localization), GO:0051338 (regulation of transferase activity), GO:0043085 (positive regulation of catalytic activity), GO:0006996 (organelle organization), GO:0010638 (positive regulation of organelle organization), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0051972 (regulation of telomerase activity), GO:0070925 (organelle assembly), GO:0033365 (protein localization to organelle), GO:0051347 (positive regulation of transferase activity), GO:0032204 (regulation of telomere maintenance), GO:0006468 (protein phosphorylation), GO:0016310 (phosphorylation), GO:2000573 (positive regulation of DNA biosynthetic process), GO:0007010 (cytoskeleton organization), GO:2001252 (positive regulation of chromosome organization), GO:0032206 (positive regulation of telomere maintenance), GO:2000278 (regulation of DNA biosynthetic process), GO:0033044 (regulation of chromosome organization), GO:0032210 (regulation of telomere maintenance via telomerase), GO:0032212 (positive regulation of telomere maintenance via telomerase), GO:1904358 (positive regulation of telomere maintenance via telomere lengthening), GO:0051973 (positive regulation of telomerase activity), GO:0140694 (non-membrane-bounded organelle assembly), GO:1904353 (regulation of telomere capping), GO:1904356 (regulation of telomere maintenance via telomere lengthening)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)