Protein: Ribosomal RNA small subunit methyltransferase H (EC 2.1.1.199) (16S rRNA m(4)C1402 methyltransferase) (rRNA (cytosine-N(4)-)-methyltransferase RsmH) (P60390)
Organism: Escherichia coli (strain K12)
Function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA. In vitro, active on the assembled 30S subunit, but not naked 16S rRNA or 70S ribosomes.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:10572301}.
Sequence: MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQLGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERFAKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEEIEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKLGGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA
InterPro domains: - IPR002903: Ribosomal RNA small subunit methyltransferase H (family) [1-312]
- IPR029063: S-adenosyl-L-methionine-dependent methyltransferase superfamily (homologous_superfamily) [7-310]
- IPR023397: S-adenosyl-L-methionine-dependent methyltransferase, MraW, recognition domain superfamily (homologous_superfamily) [112-214]
Interaction partners: - Transcriptional regulator MraZ
- Cell division protein FtsL
- Peptidoglycan D,D-transpeptidase FtsI
- UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
- UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
- Ribosomal RNA small subunit methyltransferase I
- UDP-N-acetylmuramoylalanine--D-glutamate ligase
- UDP-N-acetylglucosamine--N-acetylmuramyl-
- Probable peptidoglycan glycosyltransferase FtsW
- Phospho-N-acetylmuramoyl-pentapeptide-transferase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0005515 (protein binding), GO:0140098 (catalytic activity, acting on RNA), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0140102 (catalytic activity, acting on a rRNA), GO:0008173 (RNA methyltransferase activity), GO:0008168 (methyltransferase activity), GO:0008649 (rRNA methyltransferase activity), GO:0008757 (S-adenosylmethionine-dependent methyltransferase activity), GO:0008170 (N-methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0032259 (methylation), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0043414 (macromolecule methylation), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0001510 (RNA methylation), GO:0010467 (gene expression), GO:0030488 (tRNA methylation), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0009451 (RNA modification), GO:0034470 (ncRNA processing), GO:0006400 (tRNA modification), GO:0034660 (ncRNA metabolic process), GO:0006399 (tRNA metabolic process), GO:0008033 (tRNA processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)