Protein: Antizyme inhibitor 1 (AZI) (Ornithine decarboxylase antizyme inhibitor) (Q63764)
Organism: Rattus norvegicus (Rat)
Function: Antizyme inhibitor (AZI) protein that positively regulates ornithine decarboxylase (ODC) activity and polyamine uptake. AZI is an enzymatically inactive ODC homolog that counteracts the negative effect of ODC antizymes (AZs) OAZ1, OAZ2 and OAZ3 on ODC activity by competing with ODC for antizyme-binding. Inhibits antizyme-dependent ODC degradation and releases ODC monomers from their inactive complex with antizymes, leading to formation of the catalytically active ODC homodimer and restoring polyamine production.
Subcellular location: Nucleus {ECO:0000250}.
Sequence: MKGFIDDANYSVGLLDEGTNLGNVIDNYVYEHTLTGKNAFFVGDLGKIVKKHSQWQNVVAQIKPFYMVKCNSTPAVLEILAALGTGFACSTKNEMALVQELGVSPENIIYTSPCKQASQIKYAAKVGVNIMTCDNEVELKKIARNHPNAKVLLHIATEDNIGGEDGNMKFGTTLKNCRHLLECAKELDVQIIGVKFHISSACKEYQVYVHALSDARCVFDMAGEFGFTMNMLDIGGGFTGTEIQLEEVNHVISPLLDIYFPEGSGIQIISEPGSYYVSSAFTLAVNIIAKKVVENDKLSSGVEKNGSDEPAFVYYMNDGVYGSFASKLSEDLNTVPEVHKKYKEDEPLFTSSLWGPSCDELDQIVESCLLPELSVGDWLIFDNMGADSLHGPSAFSDTQRPAIYFMMSLSDWYEMQDAGITSDAMMKNFFFAPSCIQLSQEDNFSTEA
InterPro domains: - IPR029066: PLP-binding barrel (homologous_superfamily) [44-280]
- IPR022657: Orn/DAP/Arg decarboxylase 2, conserved site (conserved_site) [222-239]
- IPR022644: Orn/DAP/Arg decarboxylase 2, N-terminal (domain) [45-278]
- IPR022643: Orn/DAP/Arg decarboxylase 2, C-terminal (domain) [275-385]
- IPR009006: Alanine racemase/group IV decarboxylase, C-terminal (homologous_superfamily) [35-416]
- IPR031178: Antizyme inhibitor 1 (family) [26-419]
- IPR002433: Ornithine decarboxylase (family) [30-407]
- IPR000183: Ornithine/DAP/Arg decarboxylase (family) [66-287]
Interaction partners: - Ornithine decarboxylase antizyme 1
- Ornithine decarboxylase antizyme 2
- Ornithine decarboxylase antizyme 3
- Spermine synthase
- Guanidino acid hydrolase, mitochondrial
- S-adenosylmethionine decarboxylase proenzyme
- Spermidine synthase
- RCG27151, isoform CRA_a.
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0030234 (enzyme regulator activity), GO:0140677 (molecular function activator activity), GO:0008047 (enzyme activator activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009308 (amine metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006576 (biogenic amine metabolic process), GO:0009309 (amine biosynthetic process), GO:0042401 (biogenic amine biosynthetic process), GO:0006595 (polyamine metabolic process), GO:0009445 (putrescine metabolic process), GO:0006596 (polyamine biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0097708 (intracellular vesicle), GO:0005634 (nucleus)