Protein: Vacuolar amino acid transporter 3 (Q10074)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Involved in amino acid efflux from the vacuole to the cytoplasm. Capable of transporting large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine (By similarity). Required for spore formation.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:16823372}; Multi-pass membrane protein {ECO:0000269|PubMed:16823372}. Vacuole membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
Sequence: MSNSQSIKIKKPRSNENFASGSYSSRRSQQIHRLSHSPMAEGFHKPKLNISPSESNLPNNVAENTTDTPVNYGSIRDENHNSRKGKDVTLNSDEAHSENVPSTSEDPDVVRRHLGGQAADDDNFSSLQLQGGDMHRQVYRWQQEVDQNKQIRRGRSRSFSAKVSDPNLHLRSVQDMKQAGGMRRDFLRNRASSISMSSNAHGNPNFLNRNFIEFLSVYGHFAGEELSEEDEDEDTDDFAMPRDVNPSLIHSTVPSEQEPLISRHGRYKLQTPGNASNGKAVLLLLKSFVGTGVLFLPKAFKLGGLVFSSATLLIVGVLSHICFLLLIQTRMKVPGSFGDIGGTLYGPHMRFAILASIVVSQIGFSSAYISFVASTLQACVKVISTTHREYHLAVFIFIQFLVFVPLSLVRKISKLSATALIADVFILLGILYLYFWDVITLATKGIADVAMFNKTDFSLFIGVAIFTYEGICLILPIQEQMAKPKNLPKLLTGVMAAISLLFISIGLLSYAAFGSKVKTVVILNMPESTFTVIIQFLYAIAILLSTPLQLFPAIAIIEQGIFTRSGKRNRKIKWRKNYLRVLIVILAILISWAGSSRLDLFVSMVGSVCCIPLIYMYPPMLHYKACANNWILRTLDIFMFTIGAFAMAFTTYMTFF
InterPro domains: - IPR013057: Amino acid transporter, transmembrane domain (domain) [274-655]
Interaction partners: - Vacuolar amino acid transporter 5
- Putative uridine kinase C227.14
- Large ribosomal subunit protein mL61
- Autophagy-related protein 22
- 1-phosphatidylinositol 3-phosphate 5-kinase fab1
- Uncharacterized transporter C460.03
- Synaptobrevin homolog ykt6
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity), GO:0005342 (organic acid transmembrane transporter activity), GO:1901702 (salt transmembrane transporter activity), GO:0008514 (organic anion transmembrane transporter activity), GO:0046943 (carboxylic acid transmembrane transporter activity), GO:0015171 (amino acid transmembrane transporter activity), GO:0015658 (branched-chain amino acid transmembrane transporter activity), GO:0015173 (aromatic amino acid transmembrane transporter activity), GO:0015179 (L-amino acid transmembrane transporter activity), GO:0015175 (neutral L-amino acid transmembrane transporter activity), GO:0015186 (L-glutamine transmembrane transporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0055085 (transmembrane transport), GO:0051234 (establishment of localization), GO:1903825 (organic acid transmembrane transport), GO:0034486 (vacuolar transmembrane transport), GO:0006810 (transport), GO:0071705 (nitrogen compound transport), GO:0071702 (organic substance transport), GO:1905039 (carboxylic acid transmembrane transport), GO:0042886 (amide transport), GO:0015711 (organic anion transport), GO:0003333 (amino acid transmembrane transport), GO:0006865 (amino acid transport), GO:0015849 (organic acid transport), GO:0051938 (L-glutamate import), GO:0046942 (carboxylic acid transport), GO:0015807 (L-amino acid transport), GO:1902475 (L-alpha-amino acid transmembrane transport), GO:0015804 (neutral amino acid transport), GO:0015810 (aspartate transmembrane transport), GO:0015800 (acidic amino acid transport), GO:0070778 (L-aspartate transmembrane transport), GO:0006835 (dicarboxylic acid transport), GO:0015813 (L-glutamate transmembrane transport), GO:0015740 (C4-dicarboxylate transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0044232 (organelle membrane contact site), GO:0005737 (cytoplasm), GO:0031090 (organelle membrane), GO:0043229 (intracellular organelle), GO:0005773 (vacuole), GO:0043227 (membrane-bounded organelle), GO:0000323 (lytic vacuole), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0000322 (storage vacuole), GO:0005774 (vacuolar membrane), GO:0000324 (fungal-type vacuole), GO:0098852 (lytic vacuole membrane), GO:0000329 (fungal-type vacuole membrane)