Protein: Neuroendocrine convertase 2 (NEC 2) (EC 3.4.21.94) (KEX2-like endoprotease 2) (Prohormone convertase 2) (Proprotein convertase 2) (PC2) (P21661)
Organism: Mus musculus (Mouse)
Function: Serine endopeptidase which is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells (By similarity).
Subcellular location: Cytoplasmic vesicle, secretory vesicle {ECO:0000250|UniProtKB:P16519}. Secreted {ECO:0000250|UniProtKB:P16519}. Note=Localized in the secretion granules. {ECO:0000250|UniProtKB:P16519}.
Sequence: MEGGCGSQWKAAGFLFCVMVFASAERPVFTNHFLVELHKDGEEEARQVAAEHGFGVRKLPFAEGLYHFYHNGLAKAKRRRSLHHKRQLERDPRIKMALQQEGFDRKKRGYRDINEIDINMNDPLFTKQWYLFNTGQADGTPGLDLNVAEAWELGYTGKGVTIGIMDDGIDYLHPDLAYNYNADASYDFSSNDPYPYPRYTDDWFNSHGTRCAGEVSAAASNNICGVGVAYNSKVAGIRMLDQPFMTDIIEASSISHMPQLIDIYSASWGPTDNGKTVDGPRELTLQAMADGVNKGRGGKGSIYVWASGDGGSYDDCNCDGYASSMWTISINSAINDGRTALYDESCSSTLASTFSNGRKRNPEAGVATTDLYGNCTLRHSGTSAAAPEAAGVFALALEANLDLTWRDMQHLTVLTSKRNQLHDEVHQWRRNGVGLEFNHLFGYGVLDAGAMVKMAKDWKTVPERFHCVGGSVQNPEKIPPTGKLVLTLKTNACEGKENFVRYLEHVQAVITVNATRRGDLNINMTSPMGTKSILLSRRPRDDDSKVGFDKWPFMTTHTWGEDARGTWTLELGFVGSAPQKGLLKEWTLMLHGTQSAPYIDQVVRDYQSKLAMSKKQELEEELDEAVERSLQSILRKN
InterPro domains: - IPR038466: Peptidase S8, pro-domain superfamily (homologous_superfamily) [22-108]
- IPR002884: P domain (domain) [460-596]
- IPR015500: Peptidase S8, subtilisin-related (family) [157-396]
- IPR023827: Peptidase S8, subtilisin, Asp-active site (active_site) [162-173]
- IPR022398: Peptidase S8, subtilisin, His-active site (active_site) [207-217]
- IPR023828: Peptidase S8, subtilisin, Ser-active site (active_site) [381-391]
- IPR000209: Peptidase S8/S53 domain (domain) [157-444]
- IPR036852: Peptidase S8/S53 domain superfamily (homologous_superfamily) [112-458]
- IPR032815: Peptidase S8, pro-domain (domain) [32-108]
- IPR034182: Kexin/furin catalytic domain (domain) [121-417]
- IPR008979: Galactose-binding-like domain superfamily (homologous_superfamily) [452-598]
Interaction partners: - Neuroendocrine protein 7B2
- Insulin-2
- Insulin-1
- Carboxypeptidase E
- Neurogenic differentiation factor 1
- ProSAAS
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0017171 (serine hydrolase activity), GO:0008233 (peptidase activity), GO:0008236 (serine-type peptidase activity), GO:0004175 (endopeptidase activity), GO:0004252 (serine-type endopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0065008 (regulation of biological quality), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0042445 (hormone metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0016486 (peptide hormone processing), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0010817 (regulation of hormone levels), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901142 (insulin metabolic process), GO:0006518 (peptide metabolic process), GO:0006508 (proteolysis), GO:0051604 (protein maturation), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0010467 (gene expression), GO:0043043 (peptide biosynthetic process), GO:0016485 (protein processing), GO:0140448 (signaling receptor ligand precursor processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005615 (extracellular space), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0012505 (endomembrane system), GO:0005576 (extracellular region), GO:0043233 (organelle lumen), GO:0005783 (endoplasmic reticulum), GO:0030141 (secretory granule), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0060205 (cytoplasmic vesicle lumen), GO:0005788 (endoplasmic reticulum lumen), GO:0031983 (vesicle lumen), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0034774 (secretory granule lumen), GO:0070013 (intracellular organelle lumen), GO:0031982 (vesicle), GO:0097708 (intracellular vesicle)