Protein: Double-strand break repair protein MRE11 (EC 3.1.-.-) (P32829)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Involved in DNA double-strand break repair (DSBR). Possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. Also involved in meiotic DSB processing.
Subcellular location: Nucleus {ECO:0000269|PubMed:9845372}. Chromosome, telomere {ECO:0000250|UniProtKB:P49959}. Chromosome {ECO:0000250|UniProtKB:P49959}. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents. {ECO:0000250|UniProtKB:P49959}.
Sequence: MDYPDPDTIRILITTDNHVGYNENDPITGDDSWKTFHEVMMLAKNNNVDMVVQSGDLFHVNKPSKKSLYQVLKTLRLCCMGDKPCELELLSDPSQVFHYDEFTNVNYEDPNFNISIPVFGISGNHDDASGDSLLCPMDILHATGLINHFGKVIESDKIKVVPLLFQKGSTKLALYGLAAVRDERLFRTFKDGGVTFEVPTMREGEWFNLMCVHQNHTGHTNTAFLPEQFLPDFLDMVIWGHEHECIPNLVHNPIKNFDVLQPGSSVATSLCEAEAQPKYVFILDIKYGEAPKMTPIPLETIRTFKMKSISLQDVPHLRPHDKDATSKYLIEQVEEMIRDANEETKQKLADDGEGDMVAELPKPLIRLRVDYSAPSNTQSPIDYQVENPRRFSNRFVGRVANGNNVVQFYKKRSPVTRSKKSGINGTSISDRDVEKLFSESGGELEVQTLVNDLLNKMQLSLLPEVGLNEAVKKFVDKDEKTALKEFISHEISNEVGILSTNEEFLRTDDAEEMKALIKQVKRANSVRPTPPKENDETNFAFNGNGLDSFRSSNREVRTGSPDITQSHVDNESRITHISQAESSKPTSKPKRVRTATKKKIPAFSDSTVISDAENELGDNNDAQDDVDIDENDIIMVSTDEEDASYGLLNGRKTKTKTRPAASTKTASRRGKGRASRTPKTDILGSLLAKKRK
InterPro domains: - IPR038487: Mre11, capping domain (homologous_superfamily) [303-413]
- IPR041796: DNA double-strand break repair protein Mre11, N-terminal metallophosphatase domain (domain) [10-271]
- IPR004843: Calcineurin-like, phosphoesterase domain (domain) [9-245]
- IPR003701: DNA double-strand break repair protein Mre11 (family) [1-692]
- IPR029052: Metallo-dependent phosphatase-like (homologous_superfamily) [2-371]
- IPR007281: Mre11, DNA-binding (domain) [291-474]
Interaction partners: - DNA repair protein RAD50
- DNA repair protein XRS2
- Exodeoxyribonuclease 1
- Serine/threonine-protein kinase TEL1
- DNA endonuclease SAE2
- ATP-dependent DNA helicase II subunit 1
- ATP-dependent helicase SGS1
- Serine/threonine-protein kinase MEC1
- ATP-dependent DNA helicase II subunit 2
- DNA repair protein RAD51
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0043167 (ion binding), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0043169 (cation binding), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0004518 (nuclease activity), GO:0046872 (metal ion binding), GO:0046914 (transition metal ion binding), GO:0030145 (manganese ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0023052 (signaling), GO:0000003 (reproduction), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0051321 (meiotic cell cycle), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:1903046 (meiotic cell cycle process), GO:0048523 (negative regulation of cellular process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0061982 (meiosis I cell cycle process), GO:0000706 (meiotic DNA double-strand break processing), GO:1903047 (mitotic cell cycle process), GO:0035556 (intracellular signal transduction), GO:0046483 (heterocycle metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0010948 (negative regulation of cell cycle process), GO:0043170 (macromolecule metabolic process), GO:0007131 (reciprocal meiotic recombination), GO:0051726 (regulation of cell cycle), GO:1901360 (organic cyclic compound metabolic process), GO:0140013 (meiotic nuclear division), GO:0006139 (nucleobase-containing compound metabolic process), GO:0033554 (cellular response to stress), GO:0016043 (cellular component organization), GO:0045786 (negative regulation of cell cycle), GO:0044260 (cellular macromolecule metabolic process), GO:0010564 (regulation of cell cycle process), GO:0000278 (mitotic cell cycle), GO:0007346 (regulation of mitotic cell cycle), GO:0000075 (cell cycle checkpoint signaling), GO:0006974 (cellular response to DNA damage stimulus), GO:0042770 (signal transduction in response to DNA damage), GO:0007093 (mitotic cell cycle checkpoint signaling), GO:0007127 (meiosis I), GO:0042138 (meiotic DNA double-strand break formation), GO:0090304 (nucleic acid metabolic process), GO:1901987 (regulation of cell cycle phase transition), GO:0045930 (negative regulation of mitotic cell cycle), GO:0006259 (DNA metabolic process), GO:0006996 (organelle organization), GO:1901988 (negative regulation of cell cycle phase transition), GO:0031570 (DNA integrity checkpoint signaling), GO:0090305 (nucleic acid phosphodiester bond hydrolysis), GO:0000729 (DNA double-strand break processing), GO:0048285 (organelle fission), GO:0000723 (telomere maintenance), GO:0006310 (DNA recombination), GO:0006281 (DNA repair), GO:0051276 (chromosome organization), GO:0044774 (mitotic DNA integrity checkpoint signaling), GO:0000077 (DNA damage checkpoint signaling), GO:0044773 (mitotic DNA damage checkpoint signaling), GO:0000725 (recombinational repair), GO:0000280 (nuclear division), GO:0032200 (telomere organization), GO:0035825 (homologous recombination), GO:0006302 (double-strand break repair), GO:0031573 (mitotic intra-S DNA damage checkpoint signaling), GO:0000724 (double-strand break repair via homologous recombination), GO:0140527 (reciprocal homologous recombination), GO:0006303 (double-strand break repair via nonhomologous end joining)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098687 (chromosomal region), GO:0090734 (site of DNA damage), GO:0140513 (nuclear protein-containing complex), GO:0035861 (site of double-strand break), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000781 (chromosome, telomeric region), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome), GO:0005634 (nucleus)