Protein: ATP-dependent RNA helicase DHX30 (EC 3.6.4.13) (DEAH box protein 30) (Q99PU8)
Organism: Mus musculus (Mouse)
Function: RNA-dependent helicase. Plays an important role in the assembly of the mitochondrial large ribosomal subunit (By similarity). Required for optimal function of the zinc-finger antiviral protein ZC3HAV1 (By similarity). Associates with mitochondrial DNA (By similarity). Involved in nervous system development and differentiation through its involvement in the up-regulation of a number of genes which are required for neurogenesis, including GSC, NCAM1, neurogenin, and NEUROD.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:25219788}. Mitochondrion {ECO:0000250|UniProtKB:Q7L2E3}. Mitochondrion matrix, mitochondrion nucleoid {ECO:0000250|UniProtKB:Q7L2E3}. Note=Localizes to mitochondrial RNA granules found in close proximity to the mitochondrial nucleoids. Relocalizes to stress granules upon heat stress. {ECO:0000250|UniProtKB:Q7L2E3}.
Sequence: MVTPVCNSSTWQPKDSSFLSWPEMFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRKTADD
InterPro domains: - IPR011709: DEAD-box helicase, OB fold (domain) [1039-1125]
- IPR002464: DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site (conserved_site) [577-586]
- IPR007502: Helicase-associated domain (domain) [873-963]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [429-971]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [455-644]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [466-617]
- IPR056755: dsRNA binding domain (domain) [71-146]
- IPR001650: Helicase, C-terminal domain-like (domain) [677-850]
Interaction partners: - Probable ATP-dependent RNA helicase DDX28
- DNA-directed RNA polymerase, mitochondrial
- Small ribosomal subunit protein mS39
- G-rich sequence factor 1
- FAST kinase domain-containing protein 2
- FAST kinase domain-containing protein 3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0065008 (regulation of biological quality), GO:0042752 (regulation of circadian rhythm), GO:0019222 (regulation of metabolic process), GO:0060967 (negative regulation of gene silencing by RNA), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0048523 (negative regulation of cellular process), GO:0009892 (negative regulation of metabolic process), GO:1900369 (negative regulation of post-transcriptional gene silencing by RNA), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0016043 (cellular component organization), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0031647 (regulation of protein stability), GO:0031324 (negative regulation of cellular metabolic process), GO:0060965 (negative regulation of miRNA-mediated gene silencing), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010628 (positive regulation of gene expression), GO:0050821 (protein stabilization), GO:0043933 (protein-containing complex organization), GO:0051252 (regulation of RNA metabolic process), GO:0051253 (negative regulation of RNA metabolic process), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression), GO:0022607 (cellular component assembly), GO:0060966 (regulation of gene silencing by RNA), GO:0070920 (regulation of production of small RNA involved in gene silencing by RNA), GO:0065003 (protein-containing complex assembly), GO:0060147 (regulation of post-transcriptional gene silencing), GO:0071826 (ribonucleoprotein complex subunit organization), GO:1900368 (regulation of post-transcriptional gene silencing by RNA), GO:0070922 (RISC complex assembly), GO:0060964 (regulation of miRNA-mediated gene silencing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0009295 (nucleoid), GO:0005739 (mitochondrion), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0042645 (mitochondrial nucleoid), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005759 (mitochondrial matrix), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle)