Protein: V-type proton ATPase 116 kDa subunit a 1 (V-ATPase 116 kDa subunit a 1) (Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit) (Vacuolar adenosine triphosphatase subunit Ac116) (Vacuolar proton pump subunit 1) (Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1) (Q9Z1G4)
Organism: Mus musculus (Mouse)
Function: Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (By similarity). V-ATPase is responsible for the acidification of various organelles, such as lysosomes, endosomes, the trans-Golgi network, and secretory granules, including synaptic vesicles. In certain cell types, can be exported to the plasma membrane, where it is involved in the acidification of the extracellular environment (By similarity). Required for assembly and activity of the vacuolar ATPase (By similarity). Through its action on compartment acidification, plays an essential role in neuronal development in terms of integrity and connectivity of neurons.
Subcellular location: Cytoplasmic vesicle, clathrin-coated vesicle membrane {ECO:0000250|UniProtKB:P25286}; Multi-pass membrane protein {ECO:0000255}. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250|UniProtKB:P25286}; Multi-pass membrane protein {ECO:0000255}. Melanosome {ECO:0000250|UniProtKB:Q93050}.
Sequence: MGELFRSEEMTLAQLFLQSEAAYCCVSELGELGKVQFRDLNPDVNVFQRKFVNEVRRCEEMDRKLRFVEKEIRKANIPIMDTGENPEVPFPRDMIDLEANFEKIENELKEINTNQEALKRNFLELTELKFILRKTQQFFDEAELHHQQMADPDLLEESSSLLEPNEMGRGAPLRLGFVAGVINRERIPTFERMLWRVCRGNVFLRQAEIENPLEDPVTGDYVHKSVFIIFFQGDQLKNRVKKICEGFRASLYPCPETPQERKEMASGVNTRIDDLQMVLNQTEDHRQRVLQAAAKNIRVWFIKVRKMKAIYHTLNLCNIDVTQKCLIAEVWCPVTDLDSIQFALRRGTEHSGSTVPSILNRMQTNQTPPTYNKTNKFTHGFQNIVDAYGIGTYREINPAPYTVITFPFLFAVMFGDFGHGILMTLFAVWMVLRESRILSQKHENEMFSMVFSGRYIILLMGLFSIYTGLIYNDCFSKSLNIFGSSWSVRPMFTQGNWTEETLLGSSVLQLNPAIPGVFGGPYPFGIDPIWNIATNKLTFLNSFKMKMSVILGIIHMLFGVSLSLFNHIYFKKPLNIYFGFIPEIIFMSSLFGYLVILIFYKWTAYDAHSSRNAPSLLIHFINMFLFSYPESGNAMLYSGQKGIQCFLIVVAMLCVPWMLLFKPLILRHQYLRKKHLGTLNFGGIRVGNGPTEEDAEIIQHDQLSTHSEDAEEFDFGDTMVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVIHIGLHVRSLAGGLGLFFIFAAFATLTVAILLIMEGLSAFLHALRLHWVEFQNKFYTGTGFKFLPFSFEHIREGKFDE
InterPro domains: - IPR026028: ATPase, V0 complex, subunit 116kDa, eukaryotic (family) [1-838]
- IPR002490: V-type ATPase, V0 complex, 116kDa subunit family (family) [4-834]
Interaction partners: - V-type proton ATPase subunit S1
- V-type proton ATPase subunit E 1
- V-type proton ATPase 16 kDa proteolipid subunit c
- V-type proton ATPase catalytic subunit A
- V-type proton ATPase 21 kDa proteolipid subunit c''
- V-type proton ATPase subunit H
- V-type proton ATPase subunit G 2
- V-type proton ATPase subunit D
- V-type proton ATPase subunit C 1
- V-type proton ATPase subunit d 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0042592 (homeostatic process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0019725 (cellular homeostasis), GO:0055085 (transmembrane transport), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0021700 (developmental maturation), GO:0070050 (neuron cellular homeostasis), GO:0098660 (inorganic ion transmembrane transport), GO:0006810 (transport), GO:0016188 (synaptic vesicle maturation), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0034220 (monoatomic ion transmembrane transport), GO:0098655 (monoatomic cation transmembrane transport), GO:0099504 (synaptic vesicle cycle), GO:0098662 (inorganic cation transmembrane transport), GO:0016192 (vesicle-mediated transport), GO:0006996 (organelle organization), GO:0006811 (monoatomic ion transport), GO:0006812 (monoatomic cation transport), GO:0099003 (vesicle-mediated transport in synapse), GO:0016050 (vesicle organization), GO:1902600 (proton transmembrane transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:1902494 (catalytic complex), GO:0048471 (perinuclear region of cytoplasm), GO:0005737 (cytoplasm), GO:0098796 (membrane protein complex), GO:0012505 (endomembrane system), GO:1990351 (transporter complex), GO:0033177 (proton-transporting two-sector ATPase complex, proton-transporting domain), GO:1902495 (transmembrane transporter complex), GO:0030141 (secretory granule), GO:0016469 (proton-transporting two-sector ATPase complex), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:1904949 (ATPase complex), GO:0043227 (membrane-bounded organelle), GO:0033176 (proton-transporting V-type ATPase complex), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0001669 (acrosomal vesicle), GO:0031982 (vesicle), GO:0097708 (intracellular vesicle)