Protein: Carboxypeptidase Y (CPY) (EC 3.4.16.5) (O13849)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Involved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate.
Subcellular location: Vacuole {ECO:0000269|PubMed:9209031}. Note=Lysosome-like vacuoles.
Sequence: MLMKQTFLYFLLTCVVSAQFNGYVPPEQNGGDIVVPKDFYEKFGEDFIREQEESSAPLMNPVPERDEAEAPHHPKGHHEFNDDFEDDTALEHPGFKDKLDSFLQPARDFLHTVSDRLDNIFDDDEDEHVREKRPHDSADEDAPRRKHGKCKGKGKHHKGKHAKGKGKKSHPKPEDDSVFFDDERPKHHEFDDEDREFPAHHEPGEHMPPPPMHHKPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPFKHHELEEHEGPEHHRGPEDKEHHKGPKDKEHHKGPKDKEHHKGPKDKEHHKGPKDKEHHKGPKDKEHHKGPKDKEHHQGPKEKHNERPEQNMQSSHELLVIEAFADLINSVPVEEIAEEFSRFLDTLGIEYYGNIPVHIQENAPKDSSIPPLFEFDDDLELSDLTPEQFAYLEMLKAEGIDPMTAFRDQSHPAKPSNAQPADSSRPYAVFSQEENGEHVNLKAFPDHTLRVKDSKPESLGIDTVKQYTGYLDVEDDRHLFFWFFESRNDPENDPVVLWLNGGPGCSSLTGLFMELGPSSINIETLKPEYNPHSWNSNASVIFLDQPINTGFSNGDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFHIAGESYAGHYIPQFAKEIMEHNQGANFFVASGYEMEKQYINLKSVLIGNGLTDPLVQYYFYGKMACESPYGPIMSQEECDRITGAYDTCAKLITGCYQTGFTPVCIGASLYCNNAMIGPFTKTGLNIYDIREECRDQEHLCYPETGAIESYLNQEFVQEALGVEYDYKGCNTEVNIGFLFKGDWMRKTFRDDVTAILEAGLPVLIYAGDADYICNYMGNEAWTDALEWAGQREFYEAELKPWSPNGKEAGRGKSFKNFGYLRLYEAGHMVPFNQPEASLEMLNSWIDGSLFA
InterPro domains: - IPR029058: Alpha/Beta hydrolase fold (homologous_superfamily) [585-999]
- IPR001563: Peptidase S10, serine carboxypeptidase (family) [580-998]
- IPR018202: Serine carboxypeptidase, serine active site (active_site) [711-718]
Interaction partners: - Vacuolar protein sorting/targeting protein 10
- Uncharacterized carboxypeptidase C24C9.08
- Protein OS-9 homolog
- Uncharacterized derlin-like protein C365.08c
- Syntaxin pep12
- Alkaline phosphatase
- Invertase
- t-SNARE affecting a late Golgi compartment protein 1
- Vacuolar protein sorting-associated protein 27
- Cell division cycle protein 48
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0017171 (serine hydrolase activity), GO:0008233 (peptidase activity), GO:0008236 (serine-type peptidase activity), GO:0008238 (exopeptidase activity), GO:0070008 (serine-type exopeptidase activity), GO:0004180 (carboxypeptidase activity), GO:0004185 (serine-type carboxypeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006508 (proteolysis), GO:0006518 (peptide metabolic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0010467 (gene expression), GO:0043043 (peptide biosynthetic process), GO:0006465 (signal peptide processing), GO:0016485 (protein processing), GO:0031638 (zymogen activation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0043233 (organelle lumen), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0005788 (endoplasmic reticulum lumen)