Protein: Protein mono-ADP-ribosyltransferase PARP16 (EC 2.4.2.-) (ADP-ribosyltransferase diphtheria toxin-like 15) (Poly [ADP-ribose] polymerase 16) (PARP-16) (Q7TMM8)
Organism: Mus musculus (Mouse)
Function: Intracellular mono-ADP-ribosyltransferase that plays a role in different processes, such as protein translation and unfolded protein response (UPR), through the mono-ADP-ribosylation of proteins involved in those processes. Acts as an inhibitor of protein translation by catalyzing mono-ADP-ribosylation of ribosomal subunits, such as RPL14 and RPS6, thereby inhibiting polysome assembly and mRNA loading. Mono-ADP-ribosylation of ribosomal subunits is promoted by NMNAT2. Involved in the unfolded protein response (UPR) by ADP-ribosylating and activating EIF2AK3 and ERN1, two important UPR effectors. May also mediate mono-ADP-ribosylation of karyopherin KPNB1 a nuclear import factor. May not modify proteins on arginine or cysteine residues compared to other mono-ADP-ribosyltransferases.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q8N5Y8}; Single-pass type IV membrane protein {ECO:0000250|UniProtKB:Q8N5Y8}.
Sequence: MQLSNRAAAREAASRDVLAADLRCSLFASALQSYKRDSVLRPFPASYARHDCKDFEALLADTGRLPNLKELLQSSRDTDKQAWDLVSWILSSKILTIHSAKKAEFEKIQQLTGAPHTPVPTPDFLFEIEYFDPANSRFYETKGERDLIYAFHGSRLENFHSIIHNGLHCHLNKTSLFGEGTYLTSDLSLALIYSPHGHGWQHSLLGPILSCVAVCEVIDHPDVKCQIKKKDSKEIDRSRARIKHSEGGEIPPKYFVVTNNQLLRVKYLLVYSQKQPKRASSQLSWLSSHWFVIMMSLYLLLLLIVSVTNSSVFHHFWNRVKR
InterPro domains: - IPR012317: Poly(ADP-ribose) polymerase, catalytic domain (domain) [77-280]
- IPR051838: Intracellular mono-ADP-ribosyltransferase ARTD/PARP (family) [9-280]
- IPR041400: PARP16 N-terminal domain (domain) [12-90]
Interaction partners: - Protein mono-ADP-ribosyltransferase PARP4
- Protein mono-ADP-ribosyltransferase PARP10
- Protein mono-ADP-ribosyltransferase PARP3
- Protein mono-ADP-ribosyltransferase PARP14
- Protein mono-ADP-ribosyltransferase PARP12
- Poly [ADP-ribose] polymerase 2
- Protein mono-ADP-ribosyltransferase PARP11
- Protein mono-ADP-ribosyltransferase PARP9
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:1990404 (NAD+-protein ADP-ribosyltransferase activity), GO:0016757 (glycosyltransferase activity), GO:0016763 (pentosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0023056 (positive regulation of signaling), GO:0007275 (multicellular organism development), GO:0042221 (response to chemical), GO:0048584 (positive regulation of response to stimulus), GO:0006807 (nitrogen compound metabolic process), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044237 (cellular metabolic process), GO:0008219 (cell death), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0006950 (response to stress), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0009967 (positive regulation of signal transduction), GO:0012501 (programmed cell death), GO:1901564 (organonitrogen compound metabolic process), GO:0035556 (intracellular signal transduction), GO:0006979 (response to oxidative stress), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0080134 (regulation of response to stress), GO:0044248 (cellular catabolic process), GO:0048731 (system development), GO:0019538 (protein metabolic process), GO:0080135 (regulation of cellular response to stress), GO:0009966 (regulation of signal transduction), GO:1901700 (response to oxygen-containing compound), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0006793 (phosphorus metabolic process), GO:2001022 (positive regulation of response to DNA damage stimulus), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0060322 (head development), GO:0010646 (regulation of cell communication), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0006810 (transport), GO:0097190 (apoptotic signaling pathway), GO:0016043 (cellular component organization), GO:0010647 (positive regulation of cell communication), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0034599 (cellular response to oxidative stress), GO:0034097 (response to cytokine), GO:0097305 (response to alcohol), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0006974 (cellular response to DNA damage stimulus), GO:0071310 (cellular response to organic substance), GO:0062197 (cellular response to chemical stress), GO:0030163 (protein catabolic process), GO:0070302 (regulation of stress-activated protein kinase signaling cascade), GO:0071702 (organic substance transport), GO:0006796 (phosphate-containing compound metabolic process), GO:0048666 (neuron development), GO:0036211 (protein modification process), GO:0007420 (brain development), GO:0009057 (macromolecule catabolic process), GO:0022008 (neurogenesis), GO:0043412 (macromolecule modification), GO:0007399 (nervous system development), GO:0090304 (nucleic acid metabolic process), GO:0044265 (cellular macromolecule catabolic process), GO:0033993 (response to lipid), GO:0006915 (apoptotic process), GO:0007417 (central nervous system development), GO:0097193 (intrinsic apoptotic signaling pathway), GO:1902533 (positive regulation of intracellular signal transduction), GO:0006996 (organelle organization), GO:1901701 (cellular response to oxygen-containing compound), GO:0030182 (neuron differentiation), GO:1902531 (regulation of intracellular signal transduction), GO:0043408 (regulation of MAPK cascade), GO:0007005 (mitochondrion organization), GO:0006029 (proteoglycan metabolic process), GO:0006259 (DNA metabolic process), GO:0003012 (muscle system process), GO:0006468 (protein phosphorylation), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0051603 (proteolysis involved in protein catabolic process), GO:0071345 (cellular response to cytokine stimulus), GO:1903008 (organelle disassembly), GO:0016310 (phosphorylation), GO:0071396 (cellular response to lipid), GO:0043410 (positive regulation of MAPK cascade), GO:0048699 (generation of neurons), GO:0032872 (regulation of stress-activated MAPK cascade), GO:0070304 (positive regulation of stress-activated protein kinase signaling cascade), GO:0046328 (regulation of JNK cascade), GO:0061726 (mitochondrion disassembly), GO:1900744 (regulation of p38MAPK cascade), GO:0032874 (positive regulation of stress-activated MAPK cascade), GO:0046777 (protein autophosphorylation), GO:0046330 (positive regulation of JNK cascade)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031984 (organelle subcompartment), GO:0031967 (organelle envelope), GO:0005783 (endoplasmic reticulum), GO:0005635 (nuclear envelope), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0043231 (intracellular membrane-bounded organelle), GO:0071782 (endoplasmic reticulum tubular network), GO:0005634 (nucleus)