Protein: Ras-related protein Rab-35 (EC 3.6.5.2) (GTP-binding protein RAY) (Ras-related protein Rab-1C) (Q15286)
Organism: Homo sapiens (Human)
Function: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.
Subcellular location: Cell membrane {ECO:0000269|PubMed:16950109, ECO:0000269|PubMed:21951725, ECO:0000269|PubMed:30905672}; Lipid-anchor {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Membrane, clathrin-coated pit {ECO:0000269|PubMed:16950109}. Cytoplasmic vesicle, clathrin-coated vesicle {ECO:0000269|PubMed:16950109}. Endosome {ECO:0000269|PubMed:16950109, ECO:0000269|PubMed:21951725}. Melanosome {ECO:0000269|PubMed:17081065}. Note=Present on sorting endosomes and recycling endosome tubules (PubMed:16950109). Tends to be enriched in PIP2-positive cell membrane domains (PubMed:16950109). During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis (PubMed:16950109). Identified in stage I to stage IV melanosomes (PubMed:17081065). Colocalizes with ACAP2 and RUSC2 at the membrane protrusions of HEK293T cells (PubMed:30905672). {ECO:0000269|PubMed:16950109, ECO:0000269|PubMed:17081065, ECO:0000269|PubMed:30905672}.
Sequence: MARDYDHLFKLLIIGDSGVGKSSLLLRFADNTFSGSYITTIGVDFKIRTVEINGEKVKLQIWDTAGQERFRTITSTYYRGTHGVIVVYDVTSAESFVNVKRWLHEINQNCDDVCRILVGNKNDDPERKVVETEDAYKFAGQMGIQLFETSAKENVNVEEMFNCITELVLRAKKDNLAKQQQQQQNDVVKLTKNSKRKKRCC
InterPro domains: - IPR050227: Ras-related protein Rab (family) [8-201]
- IPR005225: Small GTP-binding domain (domain) [9-164]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [1-201]
- IPR041815: Rab35 (family) [3-201]
- IPR001806: Small GTPase (family) [1-201]
Interaction partners: - DENN domain-containing protein 1B
- DENN domain-containing protein 1A
- Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2
- TBC1 domain family member 10A
- DENN domain-containing protein 1C
- AP-4 complex accessory subunit RUSC2
- MAP kinase-activating death domain protein
- Inositol polyphosphate 5-phosphatase OCRL
- TBC1 domain family member 10B
- Fascin
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0051641 (cellular localization), GO:0050793 (regulation of developmental process), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0051093 (negative regulation of developmental process), GO:0048523 (negative regulation of cellular process), GO:0033036 (macromolecule localization), GO:0009892 (negative regulation of metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0046907 (intracellular transport), GO:0045596 (negative regulation of cell differentiation), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0006810 (transport), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0060255 (regulation of macromolecule metabolic process), GO:0016043 (cellular component organization), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0045184 (establishment of protein localization), GO:0006886 (intracellular protein transport), GO:0071705 (nitrogen compound transport), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0015031 (protein transport), GO:0072594 (establishment of protein localization to organelle), GO:0031326 (regulation of cellular biosynthetic process), GO:0071702 (organic substance transport), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0008104 (protein localization), GO:0051252 (regulation of RNA metabolic process), GO:0006996 (organelle organization), GO:0051253 (negative regulation of RNA metabolic process), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0010468 (regulation of gene expression), GO:0033365 (protein localization to organelle), GO:0006605 (protein targeting), GO:2001141 (regulation of RNA biosynthetic process), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0072665 (protein localization to vacuole), GO:0045892 (negative regulation of DNA-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0071944 (cell periphery), GO:0031984 (organelle subcompartment), GO:0005615 (extracellular space), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005576 (extracellular region), GO:0031090 (organelle membrane), GO:0030141 (secretory granule), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043230 (extracellular organelle), GO:0098791 (Golgi apparatus subcompartment), GO:0030133 (transport vesicle), GO:0005794 (Golgi apparatus), GO:0070062 (extracellular exosome), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005795 (Golgi stack), GO:0030140 (trans-Golgi network transport vesicle), GO:0098588 (bounding membrane of organelle), GO:0048770 (pigment granule), GO:0012506 (vesicle membrane), GO:0005770 (late endosome), GO:0065010 (extracellular membrane-bounded organelle), GO:0005798 (Golgi-associated vesicle), GO:0030667 (secretory granule membrane), GO:0030135 (coated vesicle), GO:0010008 (endosome membrane), GO:0031982 (vesicle), GO:0030659 (cytoplasmic vesicle membrane), GO:0005771 (multivesicular body), GO:0042470 (melanosome), GO:1903561 (extracellular vesicle), GO:0097708 (intracellular vesicle), GO:0031902 (late endosome membrane), GO:0030136 (clathrin-coated vesicle), GO:0032585 (multivesicular body membrane)