Protein: DNA-binding transcriptional regulator NtrC (Nitrogen regulation protein NR(I)) (Nitrogen regulator I) (NRI) (P0AFB8)
Organism: Escherichia coli (strain K12)
Function: Member of the two-component regulatory system NtrB/NtrC, which controls expression of the nitrogen-regulated (ntr) genes in response to nitrogen limitation. Phosphorylated NtrC binds directly to DNA and stimulates the formation of open promoter-sigma54-RNA polymerase complexes. Activates transcription of many genes and operons whose products minimize the slowing of growth under nitrogen-limiting conditions, including genes coding for glutamine synthetase (glnA), transporters, amino acid permeases and catabolic enzymes.
Subcellular location: Cytoplasm {ECO:0000305}.
Sequence: MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELGVEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
InterPro domains: - IPR003593: AAA+ ATPase domain (domain) [160-311]
- IPR025944: Sigma-54 interaction domain, conserved site (conserved_site) [353-362]
- IPR001789: Signal transduction response regulator, receiver domain (domain) [4-119]
- IPR010114: DNA-binding transcriptional regulator NtrC (family) [6-466]
- IPR009057: Homedomain-like superfamily (homologous_superfamily) [380-469]
- IPR025943: Sigma-54 interaction domain, ATP-binding site 2 (binding_site) [226-241]
- IPR002078: RNA polymerase sigma factor 54 interaction domain (domain) [140-369]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [130-382]
- IPR011006: CheY-like superfamily (homologous_superfamily) [6-185]
- IPR002197: DNA binding HTH domain, Fis-type (domain) [427-469]
- IPR058031: NorR-like, AAA+ ATPase lid domain (domain) [312-395]
- IPR025662: Sigma-54 interaction domain, ATP-binding site 1 (binding_site) [164-177]
Interaction partners: - Sensory histidine kinase/phosphatase NtrB)
- RNA polymerase sigma-54 factor
- Nitrogen regulatory protein GlnK
- Glutamine synthetase
- Ammonium transporter AmtB
- Bifunctional uridylyltransferase/uridylyl-removing enzyme
- Nitrogen regulatory protein P-II 1
- RNA polymerase sigma factor RpoD
- Nitrate/nitrite response regulator protein NarL
- Xylose operon regulatory protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:1901363 (heterocyclic compound binding), GO:0003700 (DNA-binding transcription factor activity), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0001216 (DNA-binding transcription activator activity), GO:0042802 (identical protein binding), GO:0001067 (transcription regulatory region nucleic acid binding), GO:0003677 (DNA binding), GO:0003690 (double-stranded DNA binding), GO:0043565 (sequence-specific DNA binding), GO:0000976 (transcription cis-regulatory region binding), GO:1990837 (sequence-specific double-stranded DNA binding), GO:0000987 (cis-regulatory region sequence-specific DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0010468 (regulation of gene expression), GO:0031326 (regulation of cellular biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0032993 (protein-DNA complex)