Protein: Probable ATP-dependent RNA helicase DDX4 (EC 3.6.4.13) (DEAD box protein 4) (Vasa homolog) (Vasa-like protein) (Q6GWX0)
Organism: Sus scrofa (Pig)
Function: ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs.
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:Q61496}. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:Q61496}. Note=Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. {ECO:0000250|UniProtKB:Q61496}.
Sequence: MGDEDWEAEINPHVSSYVPIFEKDGYSGENGDKFNRTTASSSEMDDGPSGRDHFMKSGFTSGRSYGKRDAGESNKRENTSTTGGFGVGKSFGNRGFSNNRFEDGDSSGFWRESTNDCEDNTTRNRGFSKRGGSRDGNKSEASGPFRRGGRGSFRGCRGGFGLGSQNSELDPDQGMQRGGGLFGSGRPAASDTGNGDTYQSRSGRGRGGYKGLNEEVVTGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQADLQVGQYSKREKLLEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD
InterPro domains: - IPR000629: ATP-dependent RNA helicase DEAD-box, conserved site (conserved_site) [442-450]
- IPR014014: RNA helicase, DEAD-box type, Q motif (domain) [286-314]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [250-677]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [305-516]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [310-488]
- IPR001650: Helicase, C-terminal domain-like (domain) [525-673]
Interaction partners: - RAN binding protein 9
- Deleted in azoospermia-like protein.
- Synaptonemal complex protein 3
- Stimulated by retinoic acid 8
- PR/SET domain 14
- Zinc finger and BTB domain containing 16
- Mast/stem cell growth factor receptor Kit
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0022414 (reproductive process), GO:0019953 (sexual reproduction), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0022412 (cellular process involved in reproduction in multicellular organism), GO:0032504 (multicellular organism reproduction), GO:0003006 (developmental process involved in reproduction), GO:0048609 (multicellular organismal reproductive process), GO:0007276 (gamete generation), GO:0007281 (germ cell development), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0007292 (female gamete generation), GO:0048477 (oogenesis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0099080 (supramolecular complex), GO:0043229 (intracellular organelle), GO:0035770 (ribonucleoprotein granule), GO:0036464 (cytoplasmic ribonucleoprotein granule), GO:0045495 (pole plasm), GO:0043228 (non-membrane-bounded organelle), GO:0043186 (P granule), GO:0060293 (germ plasm), GO:0043232 (intracellular non-membrane-bounded organelle)