Protein: Homoserine kinase (HK) (HSK) (EC 2.7.1.39) (P00547)
Organism: Escherichia coli (strain K12)
Function: Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate. Is also able to phosphorylate the hydroxy group on gamma-carbon of L-homoserine analogs when the functional group at the alpha-position is a carboxyl, an ester, or even a hydroxymethyl group. Neither L-threonine nor L-serine are substrates of the enzyme.
Subcellular location: Cytoplasm {ECO:0000305}.
Sequence: MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETFSLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACSVVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDIISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQPELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPETAQRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN
InterPro domains: - IPR013750: GHMP kinase, C-terminal domain (domain) [211-285]
- IPR014721: Small ribosomal subunit protein uS5 domain 2-type fold, subgroup (homologous_superfamily) [1-166]
- IPR006203: GHMP kinase, ATP-binding, conserved site (conserved_site) [90-101]
- IPR036554: GHMP kinase, C-terminal domain superfamily (homologous_superfamily) [167-298]
- IPR006204: GHMP kinase N-terminal domain (domain) [63-150]
- IPR020568: Ribosomal protein uS5 domain 2-type superfamily (homologous_superfamily) [1-162]
- IPR000870: Homoserine kinase (family) [1-310]
Interaction partners: - Threonine synthase
- Bifunctional aspartokinase/homoserine dehydrogenase 1
- Bifunctional aspartokinase/homoserine dehydrogenase 2
- Lysine-sensitive aspartokinase 3
- Aspartate-semialdehyde dehydrogenase
- Branched-chain-amino-acid aminotransferase
- Methionine synthase
- Cystathionine gamma-synthase
- 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
- Ketol-acid reductoisomerase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0016740 (transferase activity), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:0005515 (protein binding), GO:1901265 (nucleoside phosphate binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0032553 (ribonucleotide binding), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0016301 (kinase activity), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0032555 (purine ribonucleotide binding), GO:0017076 (purine nucleotide binding), GO:0005524 (ATP binding), GO:0032559 (adenyl ribonucleotide binding), GO:0030554 (adenyl nucleotide binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0009066 (aspartate family amino acid metabolic process), GO:0009069 (serine family amino acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0009070 (serine family amino acid biosynthetic process), GO:0006566 (threonine metabolic process), GO:0006563 (L-serine metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)