Protein: Peptidoglycan D,D-transpeptidase PbpA (EC 3.4.16.4) (Penicillin-binding protein A) (PBPA) (P9WKD1)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Transpeptidase that catalyzes cross-linking of the peptidoglycan cell wall (Probable). Required for the regulation of cell length. Plays critical roles for the survival of the pathogen inside the host. Required for both bacterial survival and formation of granuloma structures in a guinea pig infection model.
Subcellular location: Cell inner membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000255}.
Sequence: MNASLRRISVTVMALIVLLLLNATMTQVFTADGLRADPRNQRVLLDEYSRQRGQITAGGQLLAYSVATDGRFRFLRVYPNPEVYAPVTGFYSLRYSSTALERAEDPILNGSDRRLFGRRLADFFTGRDPRGGNVDTTINPRIQQAGWDAMQQGCYGPCKGAVVALEPSTGKILALVSSPSYDPNLLASHNPEVQAQAWQRLGDNPASPLTNRAISETYPPGSTFKVITTAAALAAGATETEQLTAAPTIPLPGSTAQLENYGGAPCGDEPTVSLREAFVKSCNTAFVQLGIRTGADALRSMARAFGLDSPPRPTPLQVAESTVGPIPDSAALGMTSIGQKDVALTPLANAEIAATIANGGITMRPYLVGSLKGPDLANISTTVGYQQRRAVSPQVAAKLTELMVGAEKVAQQKGAIPGVQIASKTGTAEHGTDPRHTPPHAWYIAFAPAQAPKVAVAVLVENGADRLSATGGALAAPIGRAVIEAALQGEP
InterPro domains: - IPR012338: Beta-lactamase/transpeptidase-like (homologous_superfamily) [128-490]
- IPR001460: Penicillin-binding protein, transpeptidase (domain) [160-484]
- IPR050515: Bacterial Transpeptidases and Beta-Lactamases (family) [75-490]
- IPR054120: Penicillin binding protein A, dimerisation domain (domain) [52-134]
Interaction partners: - Peptidoglycan glycosyltransferase RodA
- Serine/threonine-protein kinase PknB
- Serine/threonine-protein kinase PknA
- Serine/threonine protein phosphatase PstP
- Cell division protein FtsQ
- FHA domain-containing protein FhaB
- Penicillin-binding protein PbpB
- Probable peptidoglycan glycosyltransferase FtsW
- Cell wall synthesis protein Wag31
- PASTA domain-containing protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0097159 (organic cyclic compound binding), GO:0043167 (ion binding), GO:0005515 (protein binding), GO:0016491 (oxidoreductase activity), GO:0016614 (oxidoreductase activity, acting on CH-OH group of donors), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0043169 (cation binding), GO:0016616 (oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor), GO:0046872 (metal ion binding), GO:0017076 (purine nucleotide binding), GO:0000287 (magnesium ion binding), GO:0046914 (transition metal ion binding), GO:0030554 (adenyl nucleotide binding), GO:0008270 (zinc ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009605 (response to external stimulus), GO:0071840 (cellular component organization or biogenesis), GO:0009058 (biosynthetic process), GO:0071704 (organic substance metabolic process), GO:0044403 (biological process involved in symbiotic interaction), GO:0071554 (cell wall organization or biogenesis), GO:0051707 (response to other organism), GO:0009607 (response to biotic stimulus), GO:0044238 (primary metabolic process), GO:0051701 (biological process involved in interaction with host), GO:0075136 (response to host), GO:0044085 (cellular component biogenesis), GO:1901576 (organic substance biosynthetic process), GO:0043207 (response to external biotic stimulus), GO:0052173 (response to defenses of other organism), GO:0006629 (lipid metabolic process), GO:0042546 (cell wall biogenesis), GO:0052200 (response to host defenses), GO:0009273 (peptidoglycan-based cell wall biogenesis), GO:0008610 (lipid biosynthetic process), GO:0052572 (response to host immune response), GO:0071766 (Actinobacterium-type cell wall biogenesis), GO:0052553 (modulation by symbiont of host immune response), GO:0052559 (induction by symbiont of host immune response)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0009274 (peptidoglycan-based cell wall)