Protein: Ureidoacrylate amidohydrolase RutB (EC 3.5.1.110) (P75897)
Organism: Escherichia coli (strain K12)
Function: Hydrolyzes ureidoacrylate to form aminoacrylate and carbamate. The carbamate hydrolyzes spontaneously, thereby releasing one of the nitrogen atoms of the pyrimidine ring as ammonia and one of its carbon atoms as CO2.
Sequence: MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYFGVVLEDATHQAGPKFAQKAALFNIETFFGWVSDVETFCDALSPTSFAHIA
InterPro domains: - IPR000868: Isochorismatase-like domain (domain) [18-215]
- IPR019916: Ureidoacrylate amidohydrolase RutB (family) [1-228]
- IPR036380: Isochorismatase-like superfamily (homologous_superfamily) [5-227]
- IPR050272: Isochorismatase-like hydrolase (family) [10-223]
Interaction partners: - 3-aminoacrylate deaminase RutC
- Pyrimidine monooxygenase RutA
- FMN reductase RutF
- Putative carbamate hydrolase RutD
- Probable malonic semialdehyde reductase RutE
- Putative pyrimidine permease RutG
- Probable hydrolase YcaC
- NADP-dependent 3-hydroxy acid dehydrogenase YdfG
- HTH-type transcriptional regulator RutR
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016810 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds), GO:0016811 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0016999 (antibiotic metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0009237 (siderophore metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:0017000 (antibiotic biosynthetic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0033067 (macrolide metabolic process), GO:0030638 (polyketide metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901334 (lactone metabolic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006518 (peptide metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0019290 (siderophore biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0033068 (macrolide biosynthetic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0030639 (polyketide biosynthetic process), GO:0018958 (phenol-containing compound metabolic process), GO:0043043 (peptide biosynthetic process), GO:1901336 (lactone biosynthetic process), GO:0009712 (catechol-containing compound metabolic process), GO:0046189 (phenol-containing compound biosynthetic process), GO:0019184 (nonribosomal peptide biosynthetic process), GO:0009713 (catechol-containing compound biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)