Protein: Elongation factor G-1, mitochondrial (EF-Gmt) (mEF-G 1-1) (Elongation factor G1-1) (Q9C641)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Subcellular location: Mitochondrion {ECO:0000255|HAMAP-Rule:MF_03061, ECO:0000269|PubMed:14671022}.
Sequence: MARFPTSPAPNRLLRLFSSNKRSSSPTAALLTGDFQLIRHFSAGTAARVAKDEKEPWWKESMDKLRNIGISAHIDSGKTTLTERVLFYTGRIHEIHEVRGRDGVGAKMDSMDLEREKGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVDRQMRRYEVPRVAFINKLDRMGADPWKVLNQARAKLRHHSAAVQVPIGLEENFQGLIDLIHVKAYFFHGSSGENVVAGDIPADMEGLVAEKRRELIETVSEVDDVLAEKFLNDEPVSASELEEAIRRATIAQTFVPVFMGSAFKNKGVQPLLDGVVSFLPSPNEVNNYALDQNNNEERVTLTGSPDGPLVALAFKLEEGRFGQLTYLRVYEGVIKKGDFIINVNTGKRIKVPRLVRMHSNDMEDIQEAHAGQIVAVFGIECASGDTFTDGSVKYTMTSMNVPEPVMSLAVQPVSKDSGGQFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYKVDATVGKPRVNFRETITQRAEFDYLHKKQSGGAGQYGRVTGYVEPLPPGSKEKFEFENMIVGQAIPSGFIPAIEKGFKEAANSGSLIGHPVENLRIVLTDGASHAVDSSELAFKMAAIYAFRLCYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDSVITANVPLNNMFGYSTSLRSMTQGKGEFTMEYKEHSAVSNEVQAQLVNAYSASKATE
InterPro domains: - IPR031157: Tr-type G domain, conserved site (conserved_site) [109-124]
- IPR004161: Translation elongation factor EFTu-like, domain 2 (domain) [379-445]
- IPR005517: Translation elongation factor EFG/EF2, domain IV (domain) [533-654]
- IPR014721: Small ribosomal subunit protein uS5 domain 2-type fold, subgroup (homologous_superfamily) [539-743]
- IPR041095: Elongation Factor G, domain II (domain) [458-532]
- IPR035647: EF-G domain III/V-like (homologous_superfamily) [456-747]
- IPR000795: Translational (tr)-type GTP-binding domain (domain) [63-340]
- IPR005225: Small GTP-binding domain (domain) [64-237]
- IPR047872: Elongation factor G, domain IV (domain) [537-654]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [51-342]
- IPR009000: Translation protein, beta-barrel domain superfamily (homologous_superfamily) [304-456]
- IPR035649: EFG, domain V (domain) [659-736]
- IPR020568: Ribosomal protein uS5 domain 2-type superfamily (homologous_superfamily) [533-655]
- IPR004540: Translation elongation factor EFG/EF2 (family) [61-747]
- IPR000640: Elongation factor EFG, domain V-like (domain) [656-743]
- IPR009022: Elongation factor G, domain III (domain) [459-534]
Interaction partners: - Diphthine--ammonia ligase
- Translation elongation factor EF1B/ribosomal protein S6 family protein
- Protein STABILIZED1
- At4g01000
- Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain-containing protein
- Large ribosomal subunit protein bL28c
- Large ribosomal subunit protein uL10c
- Large ribosomal subunit protein bL31c
- At1g43860
- Small nuclear ribonucleoprotein Sm D2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0032553 (ribonucleotide binding), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0032555 (purine ribonucleotide binding), GO:0017076 (purine nucleotide binding), GO:0005524 (ATP binding), GO:0032559 (adenyl ribonucleotide binding), GO:0030554 (adenyl nucleotide binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0009628 (response to abiotic stimulus), GO:0006950 (response to stress), GO:0042221 (response to chemical), GO:0009266 (response to temperature stimulus), GO:0009409 (response to cold), GO:0010033 (response to organic substance), GO:1901700 (response to oxygen-containing compound), GO:0097305 (response to alcohol), GO:0045471 (response to ethanol)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)