Protein: Pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH7 (EC 3.6.4.13) (DEAH RNA helicase homolog PRP16) (Protein CLUMSY VEIN) (Protein EMBRYO DEFECTIVE 3011) (Protein PSR1-INTERACTING PROTEIN 1) (F4K2E9)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Involved in pre-mRNA splicing by mediating structural transitions of the spliceosome during the catalytic step. Facilitates expression of genes involved in auxin-mediated development including male-gametophyte transmission, apical-basal patterning of embryonic and gynoecium development, stamen development, phyllotactic flower positioning, and vascular development. Also involved in root-meristem maintenance and planar polarity of root-hair positioning. Acts as a component of RNA silencing that regulates distinct classes of endogenous small RNAs. Functions as a positive regulator of plant immunity.
Subcellular location: Nucleus {ECO:0000269|PubMed:25384462, ECO:0000269|PubMed:25902521}. Note=Binds to the spliceosome. {ECO:0000250|UniProtKB:P15938}.
Sequence: MGVDPFKTTETLEADKETNGGVPVKDKLTFKAPERKSRLGLDARAIEKKDNAKTEGEFKVPKKSAISVTSSLDEEDKSDVSGLDFGTENTRPVHSSRRYREKSSRSQSAQESTVTTENAGTSDISITPRTLSCTSSYERGGSNRHREEHRRDRSETPRSRQRNTYDEMDHYRRRESYRQSDRDYHGEKRRRYNSDWRTPGRSDWDDGQDEWERSPHGDRGSSYSRRPQPSPSPMLAAASPDARLASPWLDTPRSTMSSASPWDMGAPSPIPIRASGSSIRSSSSRYGGRSNQLAYSREGDLTNEGHSDEDRSQGAEEFKHEITETMRVEMEYQSDRAWYDTDEGNSLFDADSASFFLGDDASLQKKETELAKRLVRRDGSKMSLAQSKKYSQLNADNAQWEDRQLLRSGAVRGTEVQTEFDSEEERKAILLVHDTKPPFLDGRVVYTKQAEPVMPVKDPTSDMAIISRKGSGLVKEIREKQSANKSRQRFWELAGSNLGNILGIEKSAEQIDADTAVVGDDGEVDFKGEAKFAQHMKKGEAVSEFAMSKTMAEQRQYLPIFSVRDELLQVIRENQVIVVVGETGSGKTTQLTQYLHEDGYTINGIVGCTQPRRVAAMSVAKRVSEEMETELGDKIGYAIRFEDVTGPNTVIKYMTDGVLLRETLKDSDLDKYRVVVMDEAHERSLNTDVLFGILKKVVARRRDFKLIVTSATLNAQKFSNFFGSVPIFNIPGRTFPVNILYSKTPCEDYVEAAVKQAMTIHITSPPGDILIFMTGQDEIEAACFSLKERMEQLVSSSSREITNLLILPIYSQLPADLQAKIFQKPEDGARKCIVATNIAETSLTVDGIYYVIDTGYGKMKVFNPRMGMDALQVFPISRAASDQRAGRAGRTGPGTCYRLYTESAYLNEMLPSPVPEIQRTNLGNVVLLLKSLKIDNLLDFDFMDPPPQENILNSMYQLWVLGALNNVGGLTDLGWKMVEFPLDPPLAKMLLMGERLDCIDEVLTIVSMLSVPSVFFRPKERAEESDAAREKFFVPESDHLTLLNVYQQWKEHDYRGDWCNDHYLQVKGLRKAREVRSQLLDILKQLKIELRSCGPDWDIVRKAICSAYFHNSARLKGVGEYVNCRTGMPCHLHPSSALYGLGYTPDYVVYHELILTTKEYMQCATSVEPHWLAELGPMFFSVKDSDTSMLEHKKKQKEEKSGMEEEMEKLRRDQVESELRSKERERKKRAKQQQQISGPGLKKGTTFLRPKKLGL
InterPro domains: - IPR011709: DEAD-box helicase, OB fold (domain) [1100-1177]
- IPR007502: Helicase-associated domain (domain) [953-1043]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [516-1173]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [556-740]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [566-716]
- IPR048333: Helicase associated domain (HA2), winged-helix domain (domain) [957-982]
- IPR001650: Helicase, C-terminal domain-like (domain) [753-933]
Interaction partners: - Pre-mRNA-splicing factor SYF2
- Cell division cycle 5-like protein
- Tetratricopeptide repeat
- Cwf15 / Cwc15 cell cycle control family protein
- AT3G18790 protein
- U2 small nuclear ribonucleoprotein A'
- Transducin/WD40 repeat-like superfamily protein
- Pre-mRNA-processing factor 17
- Pre-mRNA splicing factor domain-containing protein
- At2g44200/F6E13.34
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0003729 (mRNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0008380 (RNA splicing), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0000398 (mRNA splicing, via spliceosome), GO:0000375 (RNA splicing, via transesterification reactions), GO:0000377 (RNA splicing, via transesterification reactions with bulged adenosine as nucleophile)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)