Protein: Oxygen-independent coproporphyrinogen III oxidase (CPO) (EC 1.3.98.3) (Coproporphyrinogen III dehydrogenase) (CPDH) (O34162)
Organism: Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) (Ralstonia eutropha)
Function: Involved in the heme biosynthesis. Catalyzes the anaerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen III to yield the vinyl groups in protoporphyrinogen IX.
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:P32131}.
Sequence: MIPSAITSPAPDRRALSDFRALAGRIDGNGPRYTSYPTADRFHNGPDLSLYHDALAACRADAPAPLSLYLHIPFCENICYYCGCNKIITRDHGRSARYVNYLGREMALVADRLGPRRQVLQSHWGGGTPTFLDPGEMRRVMALLHEHFELAAEGEHSIEIDPRRVDHARMALLAELGFNRVSLGVQDFDPEVQQAIHRIQPFEETRAVVDAARTLGFRSVSLDLIYGLPHQTAARFGRTIDQVLALRPDRLSVYSYAHLPHVFKPQRRIDENALPPAGEKLDILVSTIERLSAEGYVYIGMDHFALPDDDLAVAQREGRLQRNFQGYSTHAGYDQVGLGISAIGAIAGRYVQNARTLDEYYGALDHGRLPLARGVAMSADDHLRREIIGALMCNGVLDIPALEARHGIRFGTAFAPELADLAALGADGLVQCAPDRITVTPLGRLLVRRVAMVFDRYLREDAARPASTGAQAVAANDGAQPVRFVPRARYSRVV
InterPro domains: - IPR007197: Radical SAM (domain) [60-442]
- IPR034505: Anaerobic coproporphyrinogen-III oxidase (family) [61-460]
- IPR006638: Elp3/MiaA/NifB-like, radical SAM core domain (domain) [65-286]
- IPR010723: HemN, C-terminal (domain) [378-446]
- IPR004558: Oxygen-independent coproporphyrinogen III oxidase HemN (family) [16-468]
- IPR023404: Radical SAM, alpha/beta horseshoe (homologous_superfamily) [73-274]
Interaction partners: - Uroporphyrinogen decarboxylase
- Oxygen-dependent coproporphyrinogen-III oxidase
- Protoporphyrinogen IX oxidase
- FAD-dependent oxidoreductase
- cytochrome-c oxidase
- Cbb3-type cytochrome oxidase, monoheme subunit II
- Polyferredoxin
- OmpW family protein
- Cbb3-type cytochrome c oxidase subunit
- OmpW family protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016627 (oxidoreductase activity, acting on the CH-CH group of donors)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0042440 (pigment metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0046148 (pigment biosynthetic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0042168 (heme metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0033013 (tetrapyrrole metabolic process), GO:0006783 (heme biosynthetic process), GO:0006778 (porphyrin-containing compound metabolic process), GO:0033014 (tetrapyrrole biosynthetic process), GO:0006779 (porphyrin-containing compound biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)