Protein: tRNA (guanosine(18)-2'-O)-methyltransferase (EC 2.1.1.34) (tRNA [Gm18] methyltransferase) (P0AGJ2)
Organism: Escherichia coli (strain K12)
Function: Catalyzes the 2'-O methylation of guanosine at position 18 in tRNA. Type II methylase, which methylates only a subset of tRNA species.
Sequence: MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWATMQAAG
InterPro domains: - IPR033671: tRNA (guanosine(18)-2'-O)-methyltransferase (family) [2-204]
- IPR001537: tRNA/rRNA methyltransferase, SpoU type (domain) [20-159]
- IPR029028: Alpha/beta knot methyltransferases (homologous_superfamily) [4-174]
- IPR022724: RNA methyltransferase, SpoU/TrmH type, C-terminal domain (domain) [163-218]
- IPR029026: tRNA (guanine-N1-)-methyltransferase, N-terminal (homologous_superfamily) [1-204]
Interaction partners: - ATP-dependent DNA helicase RecG
- Bifunctional
- DNA-directed RNA polymerase subunit omega
- tRNA
- tRNA
- tRNA
- tRNA
- Ribosomal RNA large subunit methyltransferase H-methyltransferase)
- tRNA pseudouridine synthase B
- 23S rRNA
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0140098 (catalytic activity, acting on RNA), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0008173 (RNA methyltransferase activity), GO:0008168 (methyltransferase activity), GO:0140101 (catalytic activity, acting on a tRNA), GO:0062105 (RNA 2'-O-methyltransferase activity), GO:0008175 (tRNA methyltransferase activity), GO:0008171 (O-methyltransferase activity), GO:0008757 (S-adenosylmethionine-dependent methyltransferase activity), GO:0016427 (tRNA (cytosine) methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0032259 (methylation), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0043414 (macromolecule methylation), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0001510 (RNA methylation), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0030488 (tRNA methylation), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0009451 (RNA modification), GO:0006400 (tRNA modification), GO:0034470 (ncRNA processing), GO:0034660 (ncRNA metabolic process), GO:0006399 (tRNA metabolic process), GO:0008033 (tRNA processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)