Protein: Chlorophyllide a oxygenase, chloroplastic (Chlorophyll a oxygenase) (EC 1.14.13.122) (Chlorophyll b synthase) (AtCAO) (Q9MBA1)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Catalyzes a two-step oxygenase reaction involved in the synthesis of chlorophyll b. Acts specifically on the non-esterified chlorophyllide a and not on chlorophyll a.
Subcellular location: Plastid, chloroplast membrane {ECO:0000269|PubMed:15086960}; Peripheral membrane protein. Plastid, chloroplast thylakoid membrane {ECO:0000269|PubMed:15086960}; Peripheral membrane protein.
Sequence: MNAAVFSPSALSLPISFSKTRSSFLSRKKGVKGEFRVFAVFGDESGLVEKKSQWRPLFDVEDPRSKAPPYKGKFLDVNQAIEVARFDIQYLDWRARQDLLTIMILHDKVVDVLNPLAREYKSIGTVKKELAGLQEELSKAHQQVHISEARVSTALDKLAHMEELVNDRLLPGRVVTELDKPSSSTTASAVELDREKTNTGAKSLNVSGPVPPYSPHLKNFWYPVAFTADLKHDTMVPIECFEQPWVIFRGEDGKPGCVRNTCAHRACPLDLGTVNEGRIQCPYHGWEYSTDGECKKMPSTKLLKVKIKSLPCLEQEGMIWIWPGDEPPAPILPSLQPPSGFLIHAELVMDLPVEHGLLLDNLLDLAHAPFTHTSTFAKGWSVPSLVKFLTPTSGLQGYWDPYPIDMEFKPPCIVLSTIGISKPGKLEGKSTQQCATHLHQLHVCLPSSKNKTRLLYRMSLDFAPILKNLPFMEHLWRHFAEQVLNEDLRLVLGQQERMLNGANIWNLPVAYDKLGVRYRLWRNAVDRGDDKLPFSG
InterPro domains: - IPR017941: Rieske [2Fe-2S] iron-sulphur domain (domain) [221-321]
- IPR050584: Cholesterol 7-desaturase (family) [111-526]
- IPR013626: Pheophorbide a oxygenase (domain) [408-502]
- IPR015881: Aromatic-ring-hydroxylating dioxygenase ARHD, Rieske 2Fe-2S-binding site (binding_site) [262-284]
- IPR036922: Rieske [2Fe-2S] iron-sulphur domain superfamily (homologous_superfamily) [214-336]
Interaction partners: - Divinyl chlorophyllide a 8-vinyl-reductase, chloroplastic
- Protochlorophyllide reductase A, chloroplastic
- Chlorophyll synthase, chloroplastic
- Chlorophyll
- Probable chlorophyll
- Protochlorophyllide reductase C, chloroplastic
- Protochlorophyllide reductase B, chloroplastic
- Chlorophyllase-1
- Chlorophyllase-2
- Magnesium dechelatase SGRL, chloroplastic
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0005515 (protein binding), GO:0016491 (oxidoreductase activity), GO:0042802 (identical protein binding), GO:0004497 (monooxygenase activity), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0016709 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006091 (generation of precursor metabolites and energy), GO:0015979 (photosynthesis), GO:0022900 (electron transport chain), GO:0019684 (photosynthesis, light reaction), GO:0009767 (photosynthetic electron transport chain)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle)