Protein: DNA polymerase beta (EC 2.7.7.7) (5'-deoxyribose-phosphate lyase) (5'-dRP lyase) (EC 4.2.99.-) (AP lyase) (EC 4.2.99.18) (P06746)
Organism: Homo sapiens (Human)
Function: Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.
Subcellular location: Nucleus. Cytoplasm. Note=Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage.
Sequence: MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE
InterPro domains: - IPR043519: Nucleotidyltransferase superfamily (homologous_superfamily) [149-334]
- IPR028207: DNA polymerase beta, palm domain (domain) [149-262]
- IPR010996: Crossover junction endonuclease MUS81-like, HHH domain (domain) [12-82]
- IPR003583: Helix-hairpin-helix DNA-binding motif, class 1 (domain) [57-117]
- IPR002054: DNA-directed DNA polymerase X (domain) [10-334]
- IPR018944: DNA polymerase lambda, fingers domain (domain) [100-147]
- IPR029398: DNA polymerase beta, thumb domain (domain) [269-333]
- IPR037160: DNA polymerase, thumb domain superfamily (homologous_superfamily) [269-334]
- IPR002008: DNA polymerase family X, beta-like (family) [64-333]
- IPR027421: DNA polymerase lambda lyase domain superfamily (homologous_superfamily) [1-91]
- IPR022312: DNA polymerase family X (family) [3-334]
- IPR019843: DNA polymerase family X, binding site (binding_site) [179-198]
Interaction partners: - DNA repair protein XRCC1
- DNA ligase 3
- DNA repair nuclease/redox regulator APEX1
- Poly [ADP-ribose] polymerase 1
- DNA ligase 1
- Bifunctional polynucleotide phosphatase/kinase
- Poly [ADP-ribose] polymerase 2
- Flap endonuclease 1
- Tyrosyl-DNA phosphodiesterase 1
- Endonuclease 8-like 1 lyase Neil1)
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:0016740 (transferase activity), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0140097 (catalytic activity, acting on DNA), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0003677 (DNA binding), GO:0034061 (DNA polymerase activity), GO:0016779 (nucleotidyltransferase activity), GO:0003887 (DNA-directed DNA polymerase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009628 (response to abiotic stimulus), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0048523 (negative regulation of cellular process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:1903047 (mitotic cell cycle process), GO:0035556 (intracellular signal transduction), GO:0009314 (response to radiation), GO:0046483 (heterocycle metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0010948 (negative regulation of cell cycle process), GO:0043170 (macromolecule metabolic process), GO:0051726 (regulation of cell cycle), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0033554 (cellular response to stress), GO:0045786 (negative regulation of cell cycle), GO:0044260 (cellular macromolecule metabolic process), GO:0010564 (regulation of cell cycle process), GO:0000278 (mitotic cell cycle), GO:0007346 (regulation of mitotic cell cycle), GO:0000075 (cell cycle checkpoint signaling), GO:0006974 (cellular response to DNA damage stimulus), GO:0007093 (mitotic cell cycle checkpoint signaling), GO:0090304 (nucleic acid metabolic process), GO:1901987 (regulation of cell cycle phase transition), GO:0045930 (negative regulation of mitotic cell cycle), GO:0006259 (DNA metabolic process), GO:0010212 (response to ionizing radiation), GO:1901988 (negative regulation of cell cycle phase transition), GO:1902750 (negative regulation of cell cycle G2/M phase transition), GO:0031570 (DNA integrity checkpoint signaling), GO:1901991 (negative regulation of mitotic cell cycle phase transition), GO:0006281 (DNA repair), GO:0044818 (mitotic G2/M transition checkpoint), GO:0044774 (mitotic DNA integrity checkpoint signaling), GO:1902749 (regulation of cell cycle G2/M phase transition), GO:1901990 (regulation of mitotic cell cycle phase transition), GO:0000077 (DNA damage checkpoint signaling), GO:0000076 (DNA replication checkpoint signaling), GO:0044773 (mitotic DNA damage checkpoint signaling), GO:0010389 (regulation of G2/M transition of mitotic cell cycle), GO:0007095 (mitotic G2 DNA damage checkpoint signaling), GO:0010972 (negative regulation of G2/M transition of mitotic cell cycle), GO:0006302 (double-strand break repair), GO:0031573 (mitotic intra-S DNA damage checkpoint signaling)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043229 (intracellular organelle), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)