Protein: V-type proton ATPase catalytic subunit A (V-ATPase subunit A) (EC 7.1.2.2) (V-ATPase 69 kDa subunit) (Vacuolar proton pump subunit alpha) (Q76NM6)
Organism: Plasmodium falciparum (isolate 3D7)
Function: Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (By similarity). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. During the trophozoite stage, involved in the acidification of the extracellular space next to the cell membrane.
Subcellular location: Cell membrane {ECO:0000269|PubMed:10915784}; Peripheral membrane protein {ECO:0000269|PubMed:10915784}; Cytoplasmic side {ECO:0000269|PubMed:10915784}. Vacuole {ECO:0000269|PubMed:10915784}. Vesicle {ECO:0000269|PubMed:10915784}.
Sequence: MTKVAVEKEEPGVVYKVAGSLVIAENMSGTRMYELAKVGWNKLVGEIIRLEGNYAYIQVYEDTSGLSVGDPVIKTGNALSVELGPGILDNIYDGIQRPLERIANVCGDVYIYKGIDMTSLDHDKQWQFYADKKLKLNDIVTGGDIFGFVDENKLFKEHKIMAPPNAKGRLTYIAPDGSYTLKDKIFELEYQGKKYTYGLSHLWPVRDPRPVLEKVTGDTLLLTGQRVLDSLFPTVQGGTCAIPGAFGCGKTCVSQALSKYSNSEVIIYVGCGERGNEMAEILSDFPELTTKVDNEDVGIMQRTCLVANTSNMPVAAREASIYTGITLCEYFRDMGYNATMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGKVKCIGSPSRIGSITIVGAVSPPGGDFSDPVTTATMSIVQAFWGLDKKLAQRKHFPSVNWSTSFSKYVRQLEQYFDNFDQDFLSLRQKISDILQQESDLNDIVQLVGKDSLSEDQKVVMEVAKIIREDFLQQNAFSDYDYMCPLQKTVGMMRIICHFYAQCLRTLQEYDSRERKIGWGSIYNTLRPTINKITHMKFENPKNSDEYFKKYFKALEEEITVGLRNLMEK
InterPro domains: - IPR024034: ATPase, F1/V1 complex, beta/alpha subunit, C-terminal (homologous_superfamily) [450-611]
- IPR023366: ATP synthase subunit alpha, N-terminal domain-like superfamily (homologous_superfamily) [3-79]
- IPR022878: V-type ATP synthase catalytic alpha chain (family) [7-609]
- IPR055190: ATP synthase A/B type, C-terminal domain (domain) [458-544]
- IPR020003: ATPase, alpha/beta subunit, nucleotide-binding domain, active site (active_site) [440-449]
- IPR004100: ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain (domain) [14-76]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [81-453]
- IPR031686: ATPsynthase alpha/beta subunit, barrel-sandwich domain (domain) [118-206]
- IPR000194: ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain (domain) [224-449]
- IPR036121: ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain superfamily (homologous_superfamily) [6-78]
- IPR005725: ATPase, V1 complex, subunit A (family) [12-601]
Interaction partners: - V-type proton ATPase subunit H
- V-type proton ATPase subunit E, putative
- V-type proton ATPase proteolipid subunit
- V-type proton ATPase 21 kDa proteolipid subunit, putative
- V-type proton ATPase subunit F
- Vacuolar proton pump subunit B
- V-type proton ATPase subunit D, putative
- V-type proton ATPase subunit
- V-type proton ATPase subunit a, putative
- V-type proton ATPase subunit C
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005215 (transporter activity), GO:0140657 (ATP-dependent activity), GO:0042626 (ATPase-coupled transmembrane transporter activity), GO:0022857 (transmembrane transporter activity), GO:0042625 (ATPase-coupled ion transmembrane transporter activity), GO:0015075 (monoatomic ion transmembrane transporter activity), GO:0015318 (inorganic molecular entity transmembrane transporter activity), GO:0019829 (ATPase-coupled monoatomic cation transmembrane transporter activity), GO:0022804 (active transmembrane transporter activity), GO:0015399 (primary active transmembrane transporter activity), GO:0022890 (inorganic cation transmembrane transporter activity), GO:0022853 (active monoatomic ion transmembrane transporter activity), GO:0044769 (ATPase activity, coupled to transmembrane movement of ions, rotational mechanism), GO:0008324 (monoatomic cation transmembrane transporter activity), GO:0046961 (proton-transporting ATPase activity, rotational mechanism), GO:0015078 (proton transmembrane transporter activity), GO:0009678 (pyrophosphate hydrolysis-driven proton transmembrane transporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0006793 (phosphorus metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0019693 (ribose phosphate metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0090407 (organophosphate biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0009259 (ribonucleotide metabolic process), GO:0009141 (nucleoside triphosphate metabolic process), GO:0009117 (nucleotide metabolic process), GO:0046390 (ribose phosphate biosynthetic process), GO:0006163 (purine nucleotide metabolic process), GO:0072522 (purine-containing compound biosynthetic process), GO:1901293 (nucleoside phosphate biosynthetic process), GO:0009165 (nucleotide biosynthetic process), GO:0009144 (purine nucleoside triphosphate metabolic process), GO:0006164 (purine nucleotide biosynthetic process), GO:0009150 (purine ribonucleotide metabolic process), GO:0009260 (ribonucleotide biosynthetic process), GO:0009199 (ribonucleoside triphosphate metabolic process), GO:0009142 (nucleoside triphosphate biosynthetic process), GO:0009201 (ribonucleoside triphosphate biosynthetic process), GO:0009152 (purine ribonucleotide biosynthetic process), GO:0046034 (ATP metabolic process), GO:0009145 (purine nucleoside triphosphate biosynthetic process), GO:0009205 (purine ribonucleoside triphosphate metabolic process), GO:0006754 (ATP biosynthetic process), GO:0009206 (purine ribonucleoside triphosphate biosynthetic process), GO:0015986 (proton motive force-driven ATP synthesis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0098796 (membrane protein complex), GO:0098797 (plasma membrane protein complex), GO:1904949 (ATPase complex), GO:0005886 (plasma membrane), GO:0016469 (proton-transporting two-sector ATPase complex), GO:0045259 (proton-transporting ATP synthase complex)