Protein: Serine palmitoyltransferase 1 (EC 2.3.1.50) (Long chain base biosynthesis protein 1) (LCB 1) (Serine-palmitoyl-CoA transferase 1) (SPT 1) (SPT1) (O35704)
Organism: Mus musculus (Mouse)
Function: Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates. The SPTLC1-SPTLC2-SPTSSB complex displays a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme has the ability to use a broader range of acyl-CoAs (By similarity). Required for adipocyte cell viability and metabolic homeostasis.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:1317856, ECO:0000269|PubMed:26301690}; Single-pass membrane protein {ECO:0000269|PubMed:1317856}.
Sequence: MATVAEQWVLVEMVQALYEAPAYHLILEGILILWIIRLVFSKTYKLQERSDLTAKEKEELIEEWQPEPLVPPVSKNHPALNYNIVSGPPTHNIVVNGKECVNFASFNFLGLLANPRVKATAFSSLKKYGVGTCGPRGFYGTFDVHLDLEERLAKFMKTEEAIIYSYGFSTIASAIPAYSKRGDIIFVDSAACFAIQKGLQASRSDIKLFKHNDVADLERLLKEQEIEDQKNPRKARVTRRFIVVEGLYMNTGTICPLPELVKLKYKYKARIFLEESLSFGVLGEHGRGVTEHYGISIDDIDLISANMENALASVGGFCCGRSFVVDHQRLSGQGYCFSASLPPLLAAAAIEALNIMEENPDIFAVLKKKCQNIHKSLQGVSGLKVVGESLSPALHLQLEESTGSREKDVKLLQAIVDQCMDKGIALTQARYLDKEEKCLPPPSIRVVVTVEQTEEELQRAASTIREAAQAVLL
InterPro domains: - IPR015421: Pyridoxal phosphate-dependent transferase, major domain (homologous_superfamily) [114-359]
- IPR050087: 8-amino-7-oxononanoate synthase class-II (family) [23-466]
- IPR004839: Aminotransferase, class I/classII, large domain (domain) [99-464]
- IPR015424: Pyridoxal phosphate-dependent transferase (homologous_superfamily) [54-469]
- IPR015422: Pyridoxal phosphate-dependent transferase, small domain (homologous_superfamily) [93-463]
Interaction partners: - Serine palmitoyltransferase 2
- Serine palmitoyltransferase 3
- Serine palmitoyltransferase small subunit A
- 3-ketodihydrosphingosine reductase
- ORM1-like protein 3
- Serine palmitoyltransferase small subunit B
- ORM1-like protein 1
- ORM1-like protein 2
- Ceramide glucosyltransferase
- Ceramide kinase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006066 (alcohol metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0009894 (regulation of catabolic process), GO:0006629 (lipid metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0043603 (amide metabolic process), GO:0009896 (positive regulation of catabolic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:0010506 (regulation of autophagy), GO:0043604 (amide biosynthetic process), GO:0046165 (alcohol biosynthetic process), GO:0006665 (sphingolipid metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0010508 (positive regulation of autophagy), GO:0019751 (polyol metabolic process), GO:0006643 (membrane lipid metabolic process), GO:0006672 (ceramide metabolic process), GO:0031331 (positive regulation of cellular catabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0046467 (membrane lipid biosynthetic process), GO:0031329 (regulation of cellular catabolic process), GO:0008610 (lipid biosynthetic process), GO:0046519 (sphingoid metabolic process), GO:0016241 (regulation of macroautophagy), GO:0030148 (sphingolipid biosynthetic process), GO:0034311 (diol metabolic process), GO:0046513 (ceramide biosynthetic process), GO:0046173 (polyol biosynthetic process), GO:0016239 (positive regulation of macroautophagy), GO:0046512 (sphingosine biosynthetic process), GO:0034312 (diol biosynthetic process), GO:0046520 (sphingoid biosynthetic process), GO:0006670 (sphingosine metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140534 (endoplasmic reticulum protein-containing complex), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005783 (endoplasmic reticulum), GO:1990234 (transferase complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)