Protein: Phosphatidylcholine:ceramide cholinephosphotransferase 3 (EC 2.7.8.27) (Ethanolamine-phosphorylceramide synthase) (EPC synthase) (Sphingolipid synthase) (Sphingomyelin synthase) (SM synthase) (Q38E55)
Organism: Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Function: Bidirectional lipid cholinephosphotransferase capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. Directly and specifically recognizes the choline head group on the substrate. Also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Does not function strictly as a SM synthase. Essential for viability of the pathogenic bloodstream stage of this human protozoan parasite and, consequently, can be considered as potential drug target (By similarity).
Subcellular location: Membrane {ECO:0000255}; Multi-pass membrane protein {ECO:0000255}.
Sequence: MAVPPVEMYSGSFWNRMRKPLPLRTQVIRFTVVFVIVSFILAVALQITHERMPDPKVTKPLPDLGFELLTKVPGMYVLADCCIGFLNILSVFTAFKLYLLHRHCVGSGEPELPCNIPGVSRFFLSVWLCKENCRIELRNVHTIAWIRFITSYALLLLFRSVVIVMTSFPAPDDLCQNPPKIENPVKNVILTVLTAGGGSIHCGDLMYSGHTVILTLHLMFHWIYGAMVHWSFRPVVTVVAIFSYYCIVASRFHYTDDVLVAIYLTIATFIAVGHNADGAPWQLQLFIRWWPCCGANSREMTEDSQPVMVAFKSEAAGQSSRKVVDERNH
InterPro domains: - IPR045221: Sphingomyelin synthase-like (family) [20-310]
- IPR025749: Sphingomyelin synthase-like domain (domain) [201-273]
Interaction partners: - sphingolipid 4-desaturase
- Phosphatidic acid phosphatase, putative
- Phosphatidylinositol:ceramide inositolphosphotransferase
- Phosphatidylcholine:ceramide cholinephosphotransferase 4
- Phosphatidic acid phosphatase alpha, putative
- serine C-palmitoyltransferase
- Serine-palmitoyl-CoA transferase, putative
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016780 (phosphotransferase activity, for other substituted phosphate groups), GO:0017169 (CDP-alcohol phosphatidyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0006793 (phosphorus metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006644 (phospholipid metabolic process), GO:0006643 (membrane lipid metabolic process), GO:0090407 (organophosphate biosynthetic process), GO:0006672 (ceramide metabolic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0046467 (membrane lipid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0006665 (sphingolipid metabolic process), GO:0006684 (sphingomyelin metabolic process), GO:0008654 (phospholipid biosynthetic process), GO:0046513 (ceramide biosynthetic process), GO:0030148 (sphingolipid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)