Protein: ALG-2 interacting protein X (Alix) (Dd-Alix) (DdAlix) (Q8T7K0)
Organism: Dictyostelium discoideum (Social amoeba)
Function: Unknown. Required for development but not for cell death.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:15708561}. Cytoplasmic vesicle membrane {ECO:0000269|PubMed:15708561}. Endosome {ECO:0000269|PubMed:15708561}. Note=Colocalizes to large vesicle-like structures with chmp4. Colocalizes with vamp7A to endosomes but not to lysosomes.
Sequence: MLSIERKRTEKVDFSKPLTKYIKEQFSKAESDQHETQIATLNGLREDVRNLQERTETSKEMVWKYYSILSSLELRFPISENNVRISFPWTDSYRQRKSTLYSIYFERASVLFNYGSIVSQIASSTNRSNIEGVKKACNQFQLAAGVFNKLREYASLHPECSTSADFSSESLQALVTIMLAQAQECIYEKASMDNLSDSILSKLAAQVAEYYDTFNQLLNSNSLKSIVDRNWNITATVKSYLYKAISLYCHAKGLEQVSQFGEQVSRLMIAVDNINQSKVNLAKTAPIELKEIVERYVISITRYCESAKKDNDTIYHDTIPPAHKLTPIEKKPLAKALPLPEINFVDPFNSLVPFSVKEDSAYYNDQKETLLRKELDNIEFHNQSAKASLLSMGLPGSIEALDVGVPVALKEKMNVVTNEQGVSNITRLLENIQQLSDEDSAICLSAGNLLKKEEDEDNLMRATYGAQWHRTPSYTLTANLTQDYAKYTSHLQHSTKSDSFIRKKFEDHKNSIQELENQTEIIALLPTNNLPSGKIAEIASLTVLMNDLDALMANRESIAEKLKNLCKKDDITLKLLSPQKDKSLIYAEEIQKYEPLQMSLNESFLKQQKLIEDIRKENEKFTNQKSKQGNQREEILQKYANAYKVYNELKANLDEGTQFYLNFQEILNKFLNRCKDFTTGRENEKIELKRQIEAGVNPHSPLTSPSPSLQSPVNNYPNQFSSPQYHTSPNQQQQQQQQYVPSSQPPPQYSYNPQPYQPPQQFGGPLPPPQSFSAPPPPQSFTAPPPYNSNNKHY
InterPro domains: - IPR004328: BRO1 domain (domain) [1-385]
- IPR025304: ALIX V-shaped domain (domain) [404-684]
- IPR038499: BRO1 domain superfamily (homologous_superfamily) [1-352]
Interaction partners: - Charged multivesicular body protein 4
- ESCRT-I complex subunit tsg101
- Vacuolar protein sorting-associated protein 28
- Heat shock protein DnaJ family protein
- Charged multivesicular body protein 1
- Charged multivesicular body protein 6
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0051179 (localization), GO:0051234 (establishment of localization), GO:0051641 (cellular localization), GO:0071840 (cellular component organization or biogenesis), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0046907 (intracellular transport), GO:0006810 (transport), GO:0016192 (vesicle-mediated transport), GO:0016197 (endosomal transport), GO:0007034 (vacuolar transport), GO:0006996 (organelle organization), GO:0061024 (membrane organization), GO:0007041 (lysosomal transport), GO:0016050 (vesicle organization), GO:0048284 (organelle fusion), GO:0061025 (membrane fusion), GO:0008333 (endosome to lysosome transport), GO:0006906 (vesicle fusion), GO:0090174 (organelle membrane fusion)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0018995 (host cellular component), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043657 (host cell), GO:0043228 (non-membrane-bounded organelle), GO:0005773 (vacuole), GO:0033643 (host cell part), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0000331 (contractile vacuole), GO:0098588 (bounding membrane of organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0012506 (vesicle membrane), GO:0140220 (pathogen-containing vacuole), GO:0005774 (vacuolar membrane), GO:0031982 (vesicle), GO:0030659 (cytoplasmic vesicle membrane), GO:0031164 (contractile vacuolar membrane), GO:0097708 (intracellular vesicle), GO:0062159 (contractile vacuole complex)