Protein: Mannose-1-phosphate guanylyltransferase 1 (EC 2.7.7.13) (GDP-mannose pyrophosphorylase 1) (Protein CYTOKINESIS DEFECTIVE 1) (Protein EMBRYO DEFECTIVE 101) (Protein HYPERSENSITIVE TO AMMONIUM ION 1) (Protein SENSITIVE TO OZONE 1) (Protein VITAMIN C DEFECTIVE 1) (O22287)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Essential protein during embryogenesis. Catalyzes a reaction of the Smirnoff-Wheeler pathway, the major route to ascorbate biosynthesis in plants. Plays an essential role in plant growth and development and cell-wall architecture. Provides GDP-mannose, used for cell wall carbohydrate biosynthesis, protein N-glycosylation, as well as for the biosynthesis of the antioxidant ascorbate.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:23424245}. Nucleus {ECO:0000269|PubMed:23424245}.
Sequence: MKALILVGGFGTRLRPLTLSFPKPLVDFANKPMILHQIEALKAVGVDEVVLAINYQPEVMLNFLKDFETKLEIKITCSQETEPLGTAGPLALARDKLLDGSGEPFFVLNSDVISEYPLKEMLEFHKSHGGEASIMVTKVDEPSKYGVVVMEESTGRVEKFVEKPKLYVGNKINAGIYLLNPSVLDKIELRPTSIEKETFPKIAAAQGLYAMVLPGFWMDIGQPRDYITGLRLYLDSLRKKSPAKLTSGPHIVGNVLVDETATIGEGCLIGPDVAIGPGCIVESGVRLSRCTVMRGVRIKKHACISSSIIGWHSTVGQWARIENMTILGEDVHVSDEIYSNGGVVLPHKEIKSNILKPEIVM
InterPro domains: - IPR056729: Mannose-1-phosphate guanyltransferase, C-terminal domain (domain) [251-360]
- IPR050486: Mannose-1-phosphate guanyltransferase (family) [1-360]
- IPR029044: Nucleotide-diphospho-sugar transferases (homologous_superfamily) [1-312]
- IPR045233: Mannose-1-phosphate guanyltransferase, N-terminal domain (domain) [1-234]
- IPR005835: Nucleotidyl transferase domain (domain) [2-233]
Interaction partners: - ADP-glucose pyrophosphorylase family protein
- ADP-glucose pyrophosphorylase family protein
- Phosphomannomutase
- GDP-mannose 3,5-epimerase
- GDP-mannose 4,6 dehydratase 1
- GDP-mannose 4,6 dehydratase 2
- Dolichol-phosphate mannosyltransferase subunit 1
- GDP-L-galactose phosphorylase 1
- COP9 signalosome complex subunit 5b
- COP9 signalosome complex subunit 5a
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016779 (nucleotidyltransferase activity), GO:0070568 (guanylyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0009225 (nucleotide-sugar metabolic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0009226 (nucleotide-sugar biosynthetic process), GO:0019673 (GDP-mannose metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)