Protein: Long-chain fatty acid transport protein 2 (Arachidonate--CoA ligase) (EC 6.2.1.15) (Fatty acid transport protein 2) (FATP-2) (Fatty-acid-coenzyme A ligase, very long-chain 1) (Long-chain-fatty-acid--CoA ligase) (EC 6.2.1.3) (Phytanate--CoA ligase) (EC 6.2.1.24) (Solute carrier family 27 member 2) (THCA-CoA ligase) (EC 6.2.1.7) (Very long-chain acyl-CoA synthetase) (VLACS) (VLCS) (EC 6.2.1.-) (Very long-chain-fatty-acid-CoA ligase) (P97524)
Organism: Rattus norvegicus (Rat)
Function: Mediates the import of long-chain fatty acids (LCFA) into the cell by facilitating their transport across cell membranes, playing an important role in hepatic fatty acid uptake (By similarity). Also functions as an acyl-CoA ligase catalyzing the ATP-dependent formation of fatty acyl-CoA using LCFA and very-long-chain fatty acids (VLCFA) as substrates, which prevents fatty acid efflux from cells and might drive more fatty acid uptake. Plays a pivotal role in regulating available LCFA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis. Can also activate branched-chain fatty acids such as phytanic acid and pristanic acid (By similarity). May contribute to the synthesis of sphingosine-1-phosphate (By similarity). Does not activate C24 bile acids, cholate and chenodeoxycholate. In vitro, activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. However, it is not critical for THCA activation and bile synthesis in vivo (By similarity).
Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:10640429}; Multi-pass membrane protein {ECO:0000255}. Peroxisome membrane {ECO:0000269|PubMed:10640429, ECO:0000269|PubMed:8939997}; Peripheral membrane protein {ECO:0000269|PubMed:10640429}. Cell membrane {ECO:0000250|UniProtKB:O35488}; Multi-pass membrane protein {ECO:0000255}. Microsome {ECO:0000269|PubMed:10640429, ECO:0000269|PubMed:8939997}.
Sequence: MLPVLYTGLAGLLLLPLLLTCCCPYLLQDVRFFLQLANMARQVRSYRQRRPVRTILHVFLEQARKTPHKPFLLFRDETLTYAQVDRRSNQVARALHDHLGLRQGDCVALFMGNEPAYVWLWLGLLKLGCPMACLNYNIRAKSLLHCFQCCGAKVLLASPELHEAVEEVLPTLKKEGVSVFYVSRTSNTNGVDTVLDKVDGVSADPIPESWRSEVTFTTPAVYIYTSGTTGLPKAATINHHRLWYGTSLALRSGIKAHDVIYTTMPLYHSAALMIGLHGCIVVGATFALRSKFSASQFWDDCRKYNATVIQYIGELLRYLCNTPQKPNDRDHKVKIALGNGLRGDVWREFIKRFGDIHIYEFYASTEGNIGFMNYPRKIGAVGRENYLQKKVVRHELIKYDVEKDEPVRDANGYCIKVPKGEVGLLICKITELTPFFGYAGGKTQTEKKKLRDVFKKGDVYFNSGDLLMIDRENFIYFHDRVGDTFRWKGENVATTEVADIVGLVDFVEEVNVYGVPVPGHEGRIGMASIKMKENYEFNGKKLFQHISEYLPSYSRPRFLRIQDTIEITGTFKHRKVTLMEEGFNPSVIKDTLYFMDDTEKTYVPMTEDIYNAIIDKTLKL
InterPro domains: - IPR042099: ANL, N-terminal domain (homologous_superfamily) [53-480]
- IPR000873: AMP-dependent synthetase/ligase domain (domain) [60-382]
- IPR045851: AMP-binding enzyme, C-terminal domain superfamily (homologous_superfamily) [483-582]
- IPR020845: AMP-binding, conserved site (conserved_site) [222-233]
- IPR025110: AMP-binding enzyme, C-terminal domain (domain) [496-572]
Interaction partners: - Long-chain-fatty-acid--CoA ligase 1
- Peroxisomal acyl-coenzyme A oxidase 1
- Serum amyloid P-component
- Zinc-alpha-2-glycoprotein
- Fructose-1,6-bisphosphatase 1
- Peroxisomal acyl-coenzyme A oxidase 2
- Cytochrome P450, family 4, subfamily a, polypeptide 2
- Platelet glycoprotein 4
- Peroxisomal bifunctional enzyme
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0005215 (transporter activity), GO:0140657 (ATP-dependent activity), GO:0016874 (ligase activity), GO:0005319 (lipid transporter activity), GO:0015645 (fatty acid ligase activity), GO:0022857 (transmembrane transporter activity), GO:0005515 (protein binding), GO:0005342 (organic acid transmembrane transporter activity), GO:0004467 (long-chain fatty acid-CoA ligase activity), GO:0005324 (long-chain fatty acid transporter activity), GO:0016877 (ligase activity, forming carbon-sulfur bonds), GO:0008514 (organic anion transmembrane transporter activity), GO:0016878 (acid-thiol ligase activity), GO:0046943 (carboxylic acid transmembrane transporter activity), GO:0016405 (CoA-ligase activity), GO:0008028 (monocarboxylic acid transmembrane transporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0006629 (lipid metabolic process), GO:0044282 (small molecule catabolic process), GO:0006082 (organic acid metabolic process), GO:0044248 (cellular catabolic process), GO:0044255 (cellular lipid metabolic process), GO:1901575 (organic substance catabolic process), GO:0006631 (fatty acid metabolic process), GO:0016054 (organic acid catabolic process), GO:0043436 (oxoacid metabolic process), GO:0044242 (cellular lipid catabolic process), GO:0016042 (lipid catabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0046395 (carboxylic acid catabolic process), GO:0001676 (long-chain fatty acid metabolic process), GO:0009062 (fatty acid catabolic process), GO:0032787 (monocarboxylic acid metabolic process), GO:0072329 (monocarboxylic acid catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0071944 (cell periphery), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0042579 (microbody), GO:0005739 (mitochondrion), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0005777 (peroxisome)