Protein: DNA gyrase subunit B (EC 5.6.2.2) (Q5SHZ4)
Organism: Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
Function: A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner. It probably also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes. Relaxes negatively supercoiled DNA in an ATP-independent manner. At comparable concentrations T.thermophilus gyrase does not introduce as many negative supercoils into DNA as the E.coli enzyme.
Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01898}.
Sequence: MSYDASAIRVLKGLEGVRHRPAMYIGGTGVEGYHHLFKEILDNAVDEALAGYATEILVRLNEDGSLTVEDNGRGIPVDLMPEEGKPAVEVIYTTLHSGGKFEQGAYKVSGGLHGVGASVVNALSEWTVVEVFREGKHHRIAFSRGEVTEPLRVVGEAPRGKTGTRVTFKPDPEIFGNLRFDPSKIRARLREVAYLVAGLKLVFQDRQHGKEEVFLDKGGVASFAKALAEGEDLLYEKPFLIRGTHGEVEVEVGFLHTQGYNAEILTYANMIPTRDGGTHLTAFKSAYSRALNQYAKKAGLNKEKGPQPTGDDLLEGLYAVVSVKLPNPQFEGQTKGKLLNPEAGTAVGQVVYERLLEILEENPRIAKAVYEKALRAAQAREAARKARELVRRQNPLESDELPGKLADCQTENPEEAELFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNVEKAGLSKALKNAEVRAMVSAIGVGIGGDGEAHFDLEGLRYHKIIIMTDADVDGSHIRTLLLTFFYRYMRPLIERGHVYIAQPPLYRLQVGKKVEYLYSDEELQARLKELEGKHYEVQRFKGLGEMNPEQLWETTMNPEKRVLKRVELQDALEASELFEKLMGQEVAPRREFIEEHARYAELDI
InterPro domains: - IPR036890: Histidine kinase/HSP90-like ATPase superfamily (homologous_superfamily) [1-216]
- IPR002288: DNA gyrase B subunit, C-terminal (domain) [563-624]
- IPR011557: DNA gyrase, subunit B (family) [1-634]
- IPR000565: DNA topoisomerase, type IIA, subunit B (family) [3-620]
- IPR014721: Small ribosomal subunit protein uS5 domain 2-type fold, subgroup (homologous_superfamily) [217-388]
- IPR013760: DNA topoisomerase, type IIA-like domain superfamily (homologous_superfamily) [392-628]
- IPR003594: Histidine kinase/HSP90-like ATPase domain (domain) [28-174]
- IPR018522: DNA topoisomerase, type IIA, conserved site (conserved_site) [420-428]
- IPR013759: DNA topoisomerase, type IIA, subunit B, C-terminal (homologous_superfamily) [389-634]
- IPR013506: DNA topoisomerase, type IIA, subunit B, domain 2 (domain) [219-388]
- IPR034160: DNA gyrase subunit B, TOPRIM domain (domain) [416-533]
- IPR020568: Ribosomal protein uS5 domain 2-type superfamily (homologous_superfamily) [219-389]
- IPR006171: TOPRIM domain (domain) [416-534]
- IPR001241: DNA topoisomerase, type IIA (family) [32-628]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006259 (DNA metabolic process), GO:0006996 (organelle organization), GO:0090304 (nucleic acid metabolic process), GO:0006265 (DNA topological change), GO:0051276 (chromosome organization), GO:0071103 (DNA conformation change)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex)