Protein: Methyl-CpG-binding protein 2 (MeCp-2 protein) (MeCp2) (Q00566)
Organism: Rattus norvegicus (Rat)
Function: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Subcellular location: Nucleus {ECO:0000269|PubMed:16670375}. Note=Colocalized with methyl-CpG in the genome. Colocalized with TBL1X to the heterochromatin foci. {ECO:0000250|UniProtKB:P51608}.
Sequence: MVAGMLGLRKEKSEDQDLQGLKEKPLKFKKVKKDKKEDKEGKHEPLQPSAHHSAEPAEAGKAETSESSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTGRPKAAASEGVQVKRVLEKSPGKLLVKMPFQASPGGKGEGGGATTSAQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHAESPKAPMPLLPPPPPPEPQSSEDPISPPEPQDLSSSICKEEKMPRAGSLESDGCPKEPAKTQPMVAAAATTTTTTTTTVAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS
InterPro domains: - IPR016177: DNA-binding domain superfamily (homologous_superfamily) [73-187]
- IPR045138: Methyl-CpG binding protein MeCP2/MBD4 (family) [10-438]
- IPR017353: Methyl-CpG binding protein MeCP2 (family) [1-492]
- IPR001739: Methyl-CpG DNA binding (domain) [90-169]
Interaction partners: - Histone deacetylase 1
- Paired amphipathic helix protein Sin3a
- Histone deacetylase 2
- Nuclear receptor corepressor 1
- DNA
- Cyclic AMP-responsive element-binding protein 1
- Histone-lysine N-methyltransferase
- Delta
- Transcriptional regulator ATRX
- Transcriptional regulator ATRX.
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003682 (chromatin binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0051641 (cellular localization), GO:0050793 (regulation of developmental process), GO:0009628 (response to abiotic stimulus), GO:0050794 (regulation of cellular process), GO:0051234 (establishment of localization), GO:0051716 (cellular response to stimulus), GO:0071840 (cellular component organization or biogenesis), GO:0051241 (negative regulation of multicellular organismal process), GO:0051239 (regulation of multicellular organismal process), GO:0006950 (response to stress), GO:0051093 (negative regulation of developmental process), GO:0048523 (negative regulation of cellular process), GO:0033036 (macromolecule localization), GO:0009991 (response to extracellular stimulus), GO:2000026 (regulation of multicellular organismal development), GO:0046907 (intracellular transport), GO:0042594 (response to starvation), GO:0051656 (establishment of organelle localization), GO:0051961 (negative regulation of nervous system development), GO:0045596 (negative regulation of cell differentiation), GO:0006810 (transport), GO:0070482 (response to oxygen levels), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0045595 (regulation of cell differentiation), GO:0016043 (cellular component organization), GO:0031668 (cellular response to extracellular stimulus), GO:0009612 (response to mechanical stimulus), GO:0071496 (cellular response to external stimulus), GO:0045184 (establishment of protein localization), GO:0006886 (intracellular protein transport), GO:0071705 (nitrogen compound transport), GO:0015031 (protein transport), GO:0009267 (cellular response to starvation), GO:0072594 (establishment of protein localization to organelle), GO:0031667 (response to nutrient levels), GO:0036293 (response to decreased oxygen levels), GO:0071702 (organic substance transport), GO:0051169 (nuclear transport), GO:0008104 (protein localization), GO:0051960 (regulation of nervous system development), GO:0045665 (negative regulation of neuron differentiation), GO:0031669 (cellular response to nutrient levels), GO:0006606 (protein import into nucleus), GO:0006913 (nucleocytoplasmic transport), GO:0033365 (protein localization to organelle), GO:0050767 (regulation of neurogenesis), GO:0034504 (protein localization to nucleus), GO:0051170 (import into nucleus)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000785 (chromatin), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0000792 (heterochromatin), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005694 (chromosome), GO:0005634 (nucleus)