Protein: NAD-capped RNA hydrolase (DeNADding enzyme) (EC 3.6.1.-) (Probable NADH pyrophosphatase) (EC 3.6.1.22) (Q9Y7J0)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: mRNA decapping enzyme that specifically removes the nicotinamide adenine dinucleotide (NAD) cap from a subset of mRNAs by hydrolyzing the diphosphate linkage to produce nicotinamide mononucleotide (NMN) and 5' monophosphate mRNA. The NAD-cap is present at the 5'-end of some RNAs; in contrast to the canonical N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay (By similarity). Mediates the hydrolysis of some nucleoside diphosphate derivatives (By similarity).
Sequence: MQGFKIARHFELPTAPSQFFAGSSLNRLSFLRSNREFLNKAFYDHTTRFLPFCDLNPALLVKDDKLVTLSYPQISKYFTFSPFEHTDKQIAERFSKGESLPVLVYMGNEERNGPTDNWSQHNVFAIDITGIDELQQSIRDNGGTFVNLRSIFTEQYQLSASDSGACAFARSILDWISRYRFCPGCGKRNIPTMGGTKLVCSDVLLNDDSNCPSKKGINNYQYPRTDPCVIMVILSHDMQHILLGRALRHPKGLYACLAGFLEPGESLEEAVVRETYEESGVDVEKVLYYASQPWPFPQSLMLACFGIARKNAKIQRDKDLELEDVRFFSREEVLRSLEWDAKDGPAPILFPPKLSIARNLIQAFAYDDWTNSQVKM
InterPro domains: - IPR020084: NUDIX hydrolase, conserved site (conserved_site) [259-280]
- IPR000086: NUDIX hydrolase domain (domain) [223-351]
- IPR015375: NADH pyrophosphatase-like, N-terminal (domain) [60-175]
- IPR049734: NADH pyrophosphatase-like, C-terminal domain (domain) [226-365]
- IPR050241: NAD-capped RNA hydrolase NudC subfamily (family) [15-372]
- IPR015797: NUDIX hydrolase-like domain superfamily (homologous_superfamily) [163-364]
Interaction partners: - Putative glutamine-dependent NAD
- Probable nicotinate phosphoribosyltransferase
- Uncharacterized protein C24B11.05
- Nicotinamide/nicotinic acid mononucleotide adenylyltransferase
- NAD-dependent protein deacetylase hst2
- NAD-dependent histone deacetylase sir2
- NAD-dependent protein deacetylase hst4
- Uncharacterized kinase C24B10.02c
- IMP-specific 5'-nucleotidase 1
- Uncharacterized kinase C3H5.11
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0016462 (pyrophosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0044238 (primary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0019637 (organophosphate metabolic process), GO:0006793 (phosphorus metabolic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:0072524 (pyridine-containing compound metabolic process), GO:0009117 (nucleotide metabolic process), GO:0019362 (pyridine nucleotide metabolic process), GO:0006163 (purine nucleotide metabolic process), GO:0046496 (nicotinamide nucleotide metabolic process), GO:0019674 (NAD metabolic process), GO:0006734 (NADH metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)