Protein: Zinc metalloproteinase-disintegrin-like VAP1 (Q9DGB9)
Organism: Crotalus atrox (Western diamondback rattlesnake)
Function: Zinc metalloprotease that has fibrinogenolytic and hemorrhagic activities. It induces apoptosis in vascular endothelial cells (VEC), without degrading extracellular matrix (it cannot cleave collagen) or inhibiting adhesion of VEC. VAP1-induced apoptosis is inhibited by antibodies for integrin alpha-3, alpha-6, beta-1 and CD9. Apoptosis is accompanied by severe cell fragmentation, which is controlled by caspases
Subcellular location: Secreted
Sequence: MIQVLLVTISLAVFPYQGSSVILESGNVNDYEVVYPRKVTALPKGAVQPKYEDAMQYEFKVNGEPVVLHLEKNKGLFSEDYSETHYSPDGREITTYPPVEDHCYYHGRIENDADSTASISACNGLKGHFKLQGEMYLIEPLKLPDSEAHAVFKYENVEKEDEAPKMCGVTQNWESYEPIKKASQSNLTPEQQRYLNAKKYVKLFLVADYIMYLKYGRNLTAVRTRMYDIVNVITPIYHRMNIHVALVGLEIWSNTDKIIVQSSADVTLDLFAKWRATDLLSRKSHDNAQLLTGINFNGPTAGLGYLGGICNTMYSAGIVQDHSKIHHLVAIAMAHEMGHNLGMDHDKDTCTCGTRPCVMAGALSCEASFLFSDCSQKDHREFLIKNMPQCILKKPLKTDVVSPAVCGNYFVEVGEECDCGSPRTCRDPCCDATTCKLRQGAQCAEGLCCDQCRFKGAGTECRAAKDECDMADVCTGRSAECTDRFQRNGQPCKNNNGYCYNGKCPIMADQCIALFGPGATVSQDACFQFNREGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPNSPENKNPCNIYYSPNDEDKGMVLPGTKCADRKACSNGQCVDVTTPY
InterPro domains: - IPR024079: Metallopeptidase, catalytic domain superfamily (homologous_superfamily) [185-394]
- IPR001762: Disintegrin domain (domain) [403-488]
- IPR034027: Reprolysin domain, adamalysin-type (domain) [199-392]
- IPR001590: Peptidase M12B, ADAM/reprolysin (domain) [199-395]
- IPR018358: Disintegrin, conserved site (conserved_site) [443-462]
- IPR002870: Peptidase M12B, propeptide (domain) [32-110]
- IPR006586: ADAM, cysteine-rich domain (domain) [487-606]
- IPR036436: Disintegrin domain superfamily (homologous_superfamily) [402-485]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0043167 (ion binding), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0043169 (cation binding), GO:0008233 (peptidase activity), GO:0046872 (metal ion binding), GO:0008237 (metallopeptidase activity), GO:0046914 (transition metal ion binding), GO:0008270 (zinc ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0048583 (regulation of response to stimulus), GO:1900047 (negative regulation of hemostasis), GO:0065008 (regulation of biological quality), GO:0051241 (negative regulation of multicellular organismal process), GO:0051239 (regulation of multicellular organismal process), GO:1900046 (regulation of hemostasis), GO:0048585 (negative regulation of response to stimulus), GO:0050818 (regulation of coagulation), GO:0030195 (negative regulation of blood coagulation), GO:0032101 (regulation of response to external stimulus), GO:0030193 (regulation of blood coagulation), GO:0050878 (regulation of body fluid levels), GO:0050819 (negative regulation of coagulation), GO:1903035 (negative regulation of response to wounding), GO:0080134 (regulation of response to stress), GO:0032102 (negative regulation of response to external stimulus), GO:0061045 (negative regulation of wound healing), GO:1903034 (regulation of response to wounding), GO:0061041 (regulation of wound healing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005576 (extracellular region)