Protein: Tubulin polyglutamylase ttll-5 (EC 6.-.-.-) (Tubulin--tyrosine ligase-like protein 5) (E9P886)
Organism: Caenorhabditis elegans
Function: Polyglutamylase which preferentially modifies alpha-tubulin (By similarity). Involved in the side-chain initiation step of the polyglutamylation reaction rather than in the elongation step (By similarity). Together with ttll-4 and ttll-11, required for male mating. Probably by regulating microtubule stability via the glutamylation of tubulin, negatively regulates axon regrowth after injury in PLM neurons.
Sequence: MKPKNVPREIFNPFTNKTSLGTRREGLFLCKRLKLRPDNYLEEYCADLEVIDIMAPIRPADETERCPPTSTSDDQEAGDYLVSRNKKSRNSDYMLFSSDALVHIAHDAKVSEKYTWLGERLRLTFKMMRSDSRLIRTMCHSHGFMQCSSKNPSVNVIWMGAPVKSVRMRELMPWQRLNQFPRSTELTKKDRLYENIERSKSIFGESFDFIPEFYVTPRENRKMENAFVRVAKEIAAAGGELCFPGEFIVKPTNSRQGKGIFFANSMADIPAEGPLLVSRYLKDPYLVNNHKFDLRIYVAVTSFYPLVAYVYSEGLARLASRPYDTSASSADSNEYVHLTNYSINKNSTSFVRNESMSSEDLGHKWTLGALLRYVENEGKDAKLLMLRIEDLIVKSLLSIQNSVATASRTNLRFACTNFELFGFDVLVDQALKPWLLEVNLSPSLACDAPLDSLLKTRLIADLLNLACVPLLDRKIIDSVTPALRKSMNSQESESSETDDLELDPMCAKTLKRRPVGLKRSVLNKKIVSGSTSLIPNNEKKFDQIVRKAELEDGRRGDFIRVFPRNGTWGMYSPVMEDLGNEDFDERLFDEVVTKKNTKNSSGSSKASSSSASASSSSSSMHIEDLSDLFHEVMMQCDKYSSIADVPIEIREIISPWYEEASEYTKKITQEGETYACKLPVIRSTARLRTKSCAEFYEVRKVQLAKKKESEAMASKENEPIVLQAVVAKRI
InterPro domains: - IPR004344: Tubulin-tyrosine ligase/Tubulin polyglutamylase (family) [120-478]
Interaction partners: - Cytosolic carboxypeptidase 1
- Tubulin--tyrosine ligase-like protein 12
- mRNA-decapping enzyme 1
- m7GpppN-mRNA hydrolase dcap-2
- Cytosolic carboxypeptidase 6
- Spastin homolog
- Tubulin polyglutamylase ttll-11
- Alpha-tubulin N-acetyltransferase 1
- Secreted protein
- NAD
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0070739 (protein-glutamic acid ligase activity), GO:0016879 (ligase activity, forming carbon-nitrogen bonds), GO:0016881 (acid-amino acid ligase activity), GO:0070740 (tubulin-glutamic acid ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0022414 (reproductive process), GO:0019098 (reproductive behavior), GO:0071704 (organic substance metabolic process), GO:0032504 (multicellular organism reproduction), GO:0044238 (primary metabolic process), GO:0007610 (behavior), GO:0006807 (nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0007617 (mating behavior), GO:0043170 (macromolecule metabolic process), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0060179 (male mating behavior), GO:0018193 (peptidyl-amino acid modification), GO:0018200 (peptidyl-glutamic acid modification), GO:0018095 (protein polyglutamylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005815 (microtubule organizing center), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0005930 (axoneme), GO:0005737 (cytoplasm), GO:0036064 (ciliary basal body), GO:0120025 (plasma membrane bounded cell projection), GO:0043229 (intracellular organelle), GO:0099568 (cytoplasmic region), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0005929 (cilium), GO:0032838 (plasma membrane bounded cell projection cytoplasm), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0097014 (ciliary plasm), GO:0015630 (microtubule cytoskeleton)