Protein: Vinculin (Metavinculin) (Q64727)
Organism: Mus musculus (Mouse)
Function: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).
Subcellular location: Cell membrane {ECO:0000250|UniProtKB:P12003}; Peripheral membrane protein {ECO:0000250|UniProtKB:P12003}; Cytoplasmic side {ECO:0000250|UniProtKB:P12003}. Cell junction, adherens junction {ECO:0000250|UniProtKB:P12003}. Cell junction, focal adhesion {ECO:0000250|UniProtKB:P12003}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P85972}. Cell membrane, sarcolemma {ECO:0000269|PubMed:26359501}; Peripheral membrane protein {ECO:0000269|PubMed:26359501}; Cytoplasmic side {ECO:0000269|PubMed:26359501}. Cell projection, podosome {ECO:0000269|PubMed:27505886}. Note=Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. {ECO:0000250|UniProtKB:P12003}.
Sequence: MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKAQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ
InterPro domains: - IPR017997: Vinculin (family) [1-903]
- IPR000633: Vinculin, conserved site (conserved_site) [162-507]
- IPR006077: Vinculin/alpha-catenin (family) [368-1066]
- IPR036723: Alpha-catenin/vinculin-like superfamily (homologous_superfamily) [39-1061]
Interaction partners: - Talin-2
- Talin-1
- Paxillin
- Zyxin
- Breast cancer anti-estrogen resistance protein 1
- Alpha-actinin-4
- Focal adhesion kinase 1
- Filamin-A
- Vinexin
- Vasodilator-stimulated phosphoprotein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0019899 (enzyme binding), GO:0051020 (GTPase binding), GO:0031267 (small GTPase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0051641 (cellular localization), GO:0048856 (anatomical structure development), GO:0009653 (anatomical structure morphogenesis), GO:0048870 (cell motility), GO:0071840 (cellular component organization or biogenesis), GO:0051239 (regulation of multicellular organismal process), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0051050 (positive regulation of transport), GO:0050793 (regulation of developmental process), GO:0065008 (regulation of biological quality), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0048523 (negative regulation of cellular process), GO:0007165 (signal transduction), GO:0051640 (organelle localization), GO:0023056 (positive regulation of signaling), GO:0007275 (multicellular organism development), GO:0048584 (positive regulation of response to stimulus), GO:0019222 (regulation of metabolic process), GO:0032879 (regulation of localization), GO:0051234 (establishment of localization), GO:0009893 (positive regulation of metabolic process), GO:0030029 (actin filament-based process), GO:0001763 (morphogenesis of a branching structure), GO:0007154 (cell communication), GO:0048522 (positive regulation of cellular process), GO:0048729 (tissue morphogenesis), GO:0044085 (cellular component biogenesis), GO:0060548 (negative regulation of cell death), GO:0048731 (system development), GO:0044057 (regulation of system process), GO:0048513 (animal organ development), GO:0009966 (regulation of signal transduction), GO:0060255 (regulation of macromolecule metabolic process), GO:0060627 (regulation of vesicle-mediated transport), GO:0035295 (tube development), GO:0009887 (animal organ morphogenesis), GO:0046907 (intracellular transport), GO:0051656 (establishment of organelle localization), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0045595 (regulation of cell differentiation), GO:0016043 (cellular component organization), GO:0007166 (cell surface receptor signaling pathway), GO:0009790 (embryo development), GO:0051128 (regulation of cellular component organization), GO:0051962 (positive regulation of nervous system development), GO:0016477 (cell migration), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0035239 (tube morphogenesis), GO:0009967 (positive regulation of signal transduction), GO:0045597 (positive regulation of cell differentiation), GO:1903530 (regulation of secretion by cell), GO:1903829 (positive regulation of protein localization), GO:0051049 (regulation of transport), GO:0035556 (intracellular signal transduction), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0001568 (blood vessel development), GO:0060341 (regulation of cellular localization), GO:0061138 (morphogenesis of a branching epithelium), GO:0030036 (actin cytoskeleton organization), GO:0009891 (positive regulation of biosynthetic process), GO:0001525 (angiogenesis), GO:2000026 (regulation of multicellular organismal development), GO:0042127 (regulation of cell population proliferation), GO:0010941 (regulation of cell death), GO:0009888 (tissue development), GO:0010646 (regulation of cell communication), GO:0031325 (positive regulation of cellular metabolic process), GO:0006810 (transport), GO:0009889 (regulation of biosynthetic process), GO:0010647 (positive regulation of cell communication), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0051222 (positive regulation of protein transport), GO:1903532 (positive regulation of secretion by cell), GO:0060562 (epithelial tube morphogenesis), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0043069 (negative regulation of programmed cell death), GO:0043067 (regulation of programmed cell death), GO:1904951 (positive regulation of establishment of protein localization), GO:0001944 (vasculature development), GO:0070201 (regulation of establishment of protein localization), GO:0044319 (wound healing, spreading of cells), GO:0002009 (morphogenesis of an epithelium), GO:0050769 (positive regulation of neurogenesis), GO:0090257 (regulation of muscle system process), GO:0050678 (regulation of epithelial cell proliferation), GO:0010468 (regulation of gene expression), GO:0022607 (cellular component assembly), GO:0048754 (branching morphogenesis of an epithelial tube), GO:0022607 (cellular component assembly), GO:0051647 (nucleus localization), GO:0051223 (regulation of protein transport), GO:0003007 (heart morphogenesis), GO:0048514 (blood vessel morphogenesis), GO:0072359 (circulatory system development), GO:0001569 (branching involved in blood vessel morphogenesis), GO:1902533 (positive regulation of intracellular signal transduction), GO:0060674 (placenta blood vessel development), GO:0007264 (small GTPase mediated signal transduction), GO:0051252 (regulation of RNA metabolic process), GO:0048762 (mesenchymal cell differentiation), GO:0002040 (sprouting angiogenesis), GO:0007507 (heart development), GO:0061024 (membrane organization), GO:0007015 (actin filament organization), GO:0035270 (endocrine system development), GO:0007229 (integrin-mediated signaling pathway), GO:0010562 (positive regulation of phosphorus metabolic process), GO:0060415 (muscle tissue morphogenesis), GO:0048562 (embryonic organ morphogenesis), GO:0010256 (endomembrane system organization), GO:1901342 (regulation of vasculature development), GO:0032880 (regulation of protein localization), GO:0051960 (regulation of nervous system development), GO:0006811 (monoatomic ion transport), GO:0050714 (positive regulation of protein secretion), GO:0030855 (epithelial cell differentiation), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0048514 (blood vessel morphogenesis), GO:0031326 (regulation of cellular biosynthetic process), GO:0051247 (positive regulation of protein metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051046 (regulation of secretion), GO:0045765 (regulation of angiogenesis), GO:0060429 (epithelium development), GO:0006996 (organelle organization), GO:0010717 (regulation of epithelial to mesenchymal transition), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0051174 (regulation of phosphorus metabolic process), GO:1902531 (regulation of intracellular signal transduction), GO:0009792 (embryo development ending in birth or egg hatching), GO:0042981 (regulation of apoptotic process), GO:0001837 (epithelial to mesenchymal transition), GO:0006812 (monoatomic cation transport), GO:0065003 (protein-containing complex assembly), GO:0045937 (positive regulation of phosphate metabolic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0045601 (regulation of endothelial cell differentiation), GO:0007010 (cytoskeleton organization), GO:0007265 (Ras protein signal transduction), GO:0035315 (hair cell differentiation), GO:1902680 (positive regulation of RNA biosynthetic process), GO:0007009 (plasma membrane organization), GO:0031401 (positive regulation of protein modification process), GO:0061180 (mammary gland epithelium development), GO:0043408 (regulation of MAPK cascade), GO:0031399 (regulation of protein modification process), GO:0001700 (embryonic development via the syncytial blastoderm), GO:0043066 (negative regulation of apoptotic process), GO:0006355 (regulation of DNA-templated transcription), GO:0019220 (regulation of phosphate metabolic process), GO:0043122 (regulation of I-kappaB kinase/NF-kappaB signaling), GO:0061572 (actin filament bundle organization), GO:0001738 (morphogenesis of a polarized epithelium), GO:0009913 (epidermal cell differentiation), GO:0001932 (regulation of protein phosphorylation), GO:0001701 (in utero embryonic development), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:0042327 (positive regulation of phosphorylation), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0001934 (positive regulation of protein phosphorylation), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0042325 (regulation of phosphorylation), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:0045944 (positive regulation of transcription by RNA polymerase II), GO:0033138 (positive regulation of peptidyl-serine phosphorylation), GO:0033135 (regulation of peptidyl-serine phosphorylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0032432 (actin filament bundle), GO:0030018 (Z disc), GO:0071944 (cell periphery), GO:0042641 (actomyosin), GO:0030054 (cell junction), GO:0099080 (supramolecular complex), GO:0030017 (sarcomere), GO:0031674 (I band), GO:0005737 (cytoplasm), GO:0043292 (contractile fiber), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0001725 (stress fiber), GO:0097517 (contractile actin filament bundle), GO:0070161 (anchoring junction), GO:0005886 (plasma membrane), GO:0099081 (supramolecular polymer), GO:0030055 (cell-substrate junction), GO:0042383 (sarcolemma), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0099512 (supramolecular fiber), GO:0030016 (myofibril), GO:0005911 (cell-cell junction), GO:0005925 (focal adhesion), GO:0099513 (polymeric cytoskeletal fiber), GO:0005856 (cytoskeleton), GO:0044291 (cell-cell contact zone), GO:0015629 (actin cytoskeleton), GO:0014704 (intercalated disc), GO:0005884 (actin filament)