Protein: Mediator of RNA polymerase II transcription subunit 12 (Mediator complex subunit 12) (Mediator complex subunit Kohtalo) (dTRAP230) (Q9VW47)
Organism: Drosophila melanogaster (Fruit fly)
Function: Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for leg and eye development and macrochaete specification or differentiation.
Subcellular location: Nucleus {ECO:0000269|PubMed:12835386}.
Sequence: MLSMLQEKRPLKRTRLGPPDIYPQDAKQREDELTPTNVKHGFTTTPPLSDEFGTAHNSNVNASKVSAFFSGVLAKKEELMTLPDTGRKKQQINCKDNFWPVSPRRKCTVDAWFKDLAGNKPLLSLAKRAPSFNKKEEIFITLCENQVNMQRATWFIKLSAAYTLSFTESKNKKRSIYDPAAEWTGNMIKFMKELLPKLQEYYQQNHDKSSSNGTTSGSLTAAGNGPASNGSTGTSSINSVTGSSASTNVIPVPSMASPLPPIHSPANGQQAAPGGGVNAGSVMPTTGSLGGVVGGPGSSVVGGAAGAGAAVPPGSTISGIGSQFEDSRNALKYWKYCHQLSKYMYEESLLDRQEFLNWILDLLDKMRTQASFDEPLKKLVLSFALQYMHDFVQSERLCRKMAYIVSKKLAQLLNTVVEQQTIKELDEPKLQQDPYELALQEQMSCPHHRDIVLYLSTILQIITIECPTALVWSGIAAHRAPSSLLGSPLDHLPLAPSVLPMPTRCPRTNHEIRRQLRAAESDIVLRTQHAEQRWFAAKWLSAGKNQYTSVLATLDHLDTHCFDRMEHNNSIDTLYAQIFPSPTVSRRREEDQVEPRPPYEPKQDKDTVRILCEWAVSGQRWGEHRAMVVAILLDKRQIDVTSTPADQQSSDKDDKDSLASGAGLIDGLPVFQHVLMHFLDHDAPVLDEHVSSPQQRTEFTNLVQLFSALIRHDVFSHNAYMHTLISRGDLLLESVLVIKSGTTATKTSPPPPAPPPTTTHGFDDDGFGGGLDFKHNEFDDSNVDDDLDKLVQNIKEKGQQHEAPDSPKIGPPGDGETNPGGSISRHYVYTKHFPIPQDDPSMSSYSSESNQRYILLFGVGKERDEKKHAVKKMSKEIGKLFTKKFSIDVAAAGHVKKHSRNEFNFEATTSKCQQMAYFDQHVVTAQCAANVLEQLNGFALGNNNYLPVQEHVAFLFDLMELALNIYSLLELCDSLLKELPEVEHQLQLKKSNLVRSYTTSLALYIVSILRRYHSCLLLSPEQTLSVFEGVCRTIRHVSNPSECTSAERCIIAYLSDLHESCVLLQGKEQSTEYYQQLQCIKRFKDIFNTPEQLDLPPQGYNPLLLQELFMAPRRGGKLDPHWLGTLHESPANVYSFVSNALIAVCRETDNERLNDVALACAELTASCNVLSEEWIYALQSLCSGSKSPRYPHLGGQVDIGQLKTHNALAVFVCILVARHCFSLADFVSKFALPTLARSVSAGGAELSVDAEAGARLTCHLVLKLFKTLEIPQPGMYSVSTSPNPLHAVGNDFSIRLSCDRHLLVGAHKTIPIAAVLAVLKAILIVVDNAALKTPLASGSGTSSGGLGGAFGSGKRSGFNTPVHPGSTPKSNEQRPADLSQILGTSDLQLGSSLTSEPEALQQPSVGGMEQISLLEFAQAVLKQICAQEHVLERCLKNAEQLCDMIIDEMLTAKQAQRVLHMICYPEPEFNIISELDQRSMIVRILENLGQWTLRISWLDLQLMYRQSLSNNAELNVWLDTVARAAIDVFHMEEVVLPGAVKATHKPKPSTWLVAPLIAKLTPAVQGRILRVAGQVLESMNYFSKVSKSDCNSSGSGDEREKSNSCHSSNSYGLGGVPARNKKMPLDYQPFLGLILTCLKGQDEYKENLLVSLYAQLSQCLQSFAELDTIGGIDEPQAREEILDALQLRFSLVGGMFEAIQKNSTPTTDWAILLAQLVCQGVVDLSCNRELFTTVVDMLATLVHSTLVSDDERHYMNLMKKLKKEIGEKNNASIRVIRQLLPLYKQPTEVIACEHSGMDTKGNKICDIDKKQLRISDKQRISVWDILEGHKNPAPLSWVWFGAVKLERKPLTYEEAHRNLKYHTHSLVKPSSYYYEPLPLPPEDIEPVPEKICIKDEMKADTPSSVDQSPSAVVGGTGRGRGKGTTTRKRKPKNPKTPPVVNTQQQQPQLAQQPQQPQNVQQQQLQQQQQQQQHMQQQHMQQQQMQPNQMGQMPMNMPMNMQQFAPNPNNMMQQNAMLQQQQQQQMQQMGNNPMQQQLNVGGGNGQPNPQMNFMQQGPGGGGAGPQGMPGQQQQWHNAPQQQQPPQPYHNQYAPHQQNMQSNRIERPPLNANSKQALSQMLRQRQPFQQQAQQGPGGGFNPMQQQPQASQQQPGPQQQMNPNQMRQQQMNPQQNPQSVAAFNAMQQQPQQNAQQQQMNPNQQQQQQFMRGGNMRPGMAPNQMNQMNMGGQGMSQNPMMQQQIPQNMVGMVNPNANQMMQSGGAQGGNGVGVGVGVGVGGAGNNPNMGMGGMPQQGMIQQQPQQQPQQQVQFQNFQNQYQQQQQQGMQQQGGGAGVGVGVGMAPNQQQQQQANMMGNFNPQMQQGNRNNPDFMAAAAVAQQQQQQQQQQRVVPGGMMAGNRNQYMNQAPNVTMSTMMGPGPGGVVGQVPPYARQQSAGGGKPGVLNTQQQFQQQQQQQQQLRHQMMQLQGMGGGAGGGMGAGPQQGGGAVGGGAGGGMVPQQQSMNQQQTPNLVAQLQRQNMMGQQQYQPPPY
InterPro domains: - IPR051647: Mediator complex subunit 12 (family) [431-2085]
- IPR019035: Mediator complex, subunit Med12 (domain) [97-157]
- IPR021990: Mediator complex, subunit Med12, LCEWAV-domain (domain) [390-856]
Interaction partners: - Cyclin-C
- Cyclin-dependent kinase 8
- Mediator of RNA polymerase II transcription subunit 13
- Mediator of RNA polymerase II transcription subunit 14
- Mediator of RNA polymerase II transcription subunit 15
- Mediator of RNA polymerase II transcription subunit 6
- Mediator of RNA polymerase II transcription subunit 31
- Mediator of RNA polymerase II transcription subunit 1
- Mediator of RNA polymerase II transcription subunit 10
- Mediator of RNA polymerase II transcription subunit 18
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0140110 (transcription regulator activity), GO:0005488 (binding), GO:0005515 (protein binding), GO:0003712 (transcription coregulator activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050793 (regulation of developmental process), GO:0051239 (regulation of multicellular organismal process), GO:0050794 (regulation of cellular process), GO:0051726 (regulation of cell cycle), GO:2000026 (regulation of multicellular organismal development), GO:0007346 (regulation of mitotic cell cycle)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140513 (nuclear protein-containing complex), GO:0043229 (intracellular organelle), GO:0016592 (mediator complex), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)