Protein: STE20/SPS1-related proline-alanine-rich protein kinase (Ste-20-related kinase) (EC 2.7.11.1) (Pancreatic serine/threonine-protein kinase) (PS/TK) (PSTK1) (Serine/threonine-protein kinase 39) (O88506)
Organism: Rattus norvegicus (Rat)
Function: May act as a mediator of stress-activated signals. Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities by the WNK scaffolds, probably through phosphorylation. Phosphorylates RELT.
Subcellular location: Cytoplasm {ECO:0000305}. Nucleus {ECO:0000305}. Note=Nucleus when caspase-cleaved. {ECO:0000305}.
Sequence: MAEPSGSPVHVQLPQQAAPVTAAAAAPAAATSAPAPAPAPAAPAAPAPAPAAAPAPAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAASQEKSRRVKEENPEISVNAGGIPEQIQSLSVHDSQGQPNANEDYREGPCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGAEIPDEVKLIGFAQLSVS
InterPro domains: - IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [62-364]
- IPR050629: STE20/SPS1-related Proline-Alanine-rich Kinase (family) [64-526]
- IPR024678: Serine/threonine-protein kinase OSR1/WNK, CCT domain (domain) [460-517]
- IPR017441: Protein kinase, ATP binding site (binding_site) [78-101]
- IPR000719: Protein kinase domain (domain) [72-346]
Interaction partners: - Solute carrier family 12 member 2
- Solute carrier family 12 member 1-chloride cotransporter 1)
- Translocation protein SEC62
- Calcium binding protein 39
- Kelch-like protein 3
- Solute carrier family 12 member 5
- Translocation protein SEC63 homolog
- Protein transport protein Sec61 subunit beta
- Solute carrier family 12 member 7
- Protein transport protein Sec61 subunit alpha isoform 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0048583 (regulation of response to stimulus), GO:0065009 (regulation of molecular function), GO:0051051 (negative regulation of transport), GO:0048523 (negative regulation of cellular process), GO:0032879 (regulation of localization), GO:0050794 (regulation of cellular process), GO:0051049 (regulation of transport), GO:0032410 (negative regulation of transporter activity), GO:0032409 (regulation of transporter activity), GO:0044092 (negative regulation of molecular function), GO:0080134 (regulation of response to stress), GO:0043271 (negative regulation of monoatomic ion transport), GO:0034762 (regulation of transmembrane transport), GO:0034763 (negative regulation of transmembrane transport), GO:0032413 (negative regulation of ion transmembrane transporter activity), GO:0034766 (negative regulation of monoatomic ion transmembrane transport), GO:0034765 (regulation of monoatomic ion transmembrane transport), GO:0022898 (regulation of transmembrane transporter activity), GO:0043269 (regulation of monoatomic ion transport), GO:0043267 (negative regulation of potassium ion transport), GO:2001258 (negative regulation of cation channel activity), GO:1901017 (negative regulation of potassium ion transmembrane transporter activity), GO:1901380 (negative regulation of potassium ion transmembrane transport), GO:1904062 (regulation of monoatomic cation transmembrane transport), GO:0032412 (regulation of monoatomic ion transmembrane transporter activity), GO:1904063 (negative regulation of cation transmembrane transport), GO:0010959 (regulation of metal ion transport), GO:1901016 (regulation of potassium ion transmembrane transporter activity), GO:0043266 (regulation of potassium ion transport), GO:1901379 (regulation of potassium ion transmembrane transport), GO:2001257 (regulation of cation channel activity)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0045178 (basal part of cell), GO:0045177 (apical part of cell), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0044297 (cell body), GO:0009925 (basal plasma membrane), GO:0098590 (plasma membrane region), GO:0005886 (plasma membrane), GO:0016324 (apical plasma membrane), GO:0016323 (basolateral plasma membrane)