Protein: ATP-dependent dethiobiotin synthetase BioD 2 (P0A6E9)
Organism: Escherichia coli (strain K12)
Function: Catalyzes a mechanistically unusual reaction, the ATP- dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8- diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring
Subcellular location: Cytoplasm
Sequence: MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGSKETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANLTEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIANDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLAMLRSVLAVDRVTV
InterPro domains: - IPR004472: Dethiobiotin synthase BioD (family) [1-220]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [1-220]
Interaction partners: - Biotin synthase
- Adenosylmethionine-8-amino-7-oxononanoate aminotransferase
- 8-amino-7-oxononanoate synthase
- ATP-dependent dethiobiotin synthetase BioD 1
- Malonyl-[acyl-carrier protein] O-methyltransferase
- Pimeloyl-[acyl-carrier protein] methyl ester esterase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0016874 (ligase activity), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0043169 (cation binding), GO:0032553 (ribonucleotide binding), GO:0016879 (ligase activity, forming carbon-nitrogen bonds), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0032555 (purine ribonucleotide binding), GO:0046872 (metal ion binding), GO:0017076 (purine nucleotide binding), GO:0005524 (ATP binding), GO:0000287 (magnesium ion binding), GO:0032559 (adenyl ribonucleotide binding), GO:0030554 (adenyl nucleotide binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0006082 (organic acid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0006766 (vitamin metabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006767 (water-soluble vitamin metabolic process), GO:0009110 (vitamin biosynthetic process), GO:0044272 (sulfur compound biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006768 (biotin metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0042364 (water-soluble vitamin biosynthetic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0009102 (biotin biosynthetic process), GO:0072330 (monocarboxylic acid biosynthetic process), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)