Protein: ATP-dependent zinc metalloprotease FtsH (EC 3.4.24.-) (P9WQN3)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.
Subcellular location: Cell membrane {ECO:0000305|PubMed:9729123}; Multi-pass membrane protein {ECO:0000305|PubMed:9729123}; Cytoplasmic side {ECO:0000305|PubMed:9729123}. Note=Upon expression in E.coli.
Sequence: MNRKNVTRTITAIAVVVLLGWSFFYFSDDTRGYKPVDTSVAITQINGDNVKSAQIDDREQQLRLILKKGNNETDGSEKVITKYPTGYAVDLFNALSAKNAKVSTVVNQGSILGELLVYVLPLLLLVGLFVMFSRMQGGARMGFGFGKSRAKQLSKDMPKTTFADVAGVDEAVEELYEIKDFLQNPSRYQALGAKIPKGVLLYGPPGTGKTLLARAVAGEAGVPFFTISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFVDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFGDRAGVILIAATNRPDILDPALLRPGRFDRQIPVSNPDLAGRRAVLRVHSKGKPMAADADLDGLAKRTVGMTGADLANVINEAALLTARENGTVITGPALEEAVDRVIGGPRRKGRIISEQEKKITAYHEGGHTLAAWAMPDIEPIYKVTILARGRTGGHAVAVPEEDKGLRTRSEMIAQLVFAMGGRAAEELVFREPTTGAVSDIEQATKIARSMVTEFGMSSKLGAVKYGSEHGDPFLGRTMGTQPDYSHEVAREIDEEVRKLIEAAHTEAWEILTEYRDVLDTLAGELLEKETLHRPELESIFADVEKRPRLTMFDDFGGRIPSDKPPIKTPGELAIERGEPWPQPVPEPAFKAAIAQATQAAEAARSDAGQTGHGANGSPAGTHRSGDRQYGSTQPDYGAPAGWHAPGWPPRSSHRPSYSGEPAPTYPGQPYPTGQADPGSDESSAEQDDEVSRTKPAHG
InterPro domains: - IPR000642: Peptidase M41 (domain) [412-601]
- IPR003593: AAA+ ATPase domain (domain) [195-334]
- IPR041569: AAA ATPase, AAA+ lid domain (domain) [354-394]
- IPR003959: ATPase, AAA-type, core (domain) [199-330]
- IPR037219: Peptidase M41-like (homologous_superfamily) [413-614]
- IPR011546: Peptidase M41, FtsH extracellular (domain) [11-105]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [150-403]
- IPR005936: ATP-dependent zinc metalloprotease, FtsH (family) [8-610]
- IPR003960: ATPase, AAA-type, conserved site (conserved_site) [302-320]
Interaction partners: - GTP cyclohydrolase 1
- Dihydroneopterin aldolase
- Uncharacterized protein Rv1488
- Protease Rv3090
- Dihydropteroate synthase
- Large ribosomal subunit protein uL13
- Large ribosomal subunit protein bL19
- Large ribosomal subunit protein bL17
- Large ribosomal subunit protein uL3
- Large ribosomal subunit protein uL2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0008233 (peptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:1901575 (organic substance catabolic process), GO:0043170 (macromolecule metabolic process), GO:0006091 (generation of precursor metabolites and energy), GO:0015979 (photosynthesis), GO:0009057 (macromolecule catabolic process), GO:0030091 (protein repair), GO:0009765 (photosynthesis, light harvesting), GO:1901565 (organonitrogen compound catabolic process), GO:0030163 (protein catabolic process), GO:0015980 (energy derivation by oxidation of organic compounds), GO:0045333 (cellular respiration), GO:0044260 (cellular macromolecule metabolic process), GO:0062012 (regulation of small molecule metabolic process), GO:0019646 (aerobic electron transport chain), GO:0009060 (aerobic respiration), GO:1902106 (negative regulation of leukocyte differentiation), GO:0045595 (regulation of cell differentiation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0005886 (plasma membrane), GO:0009274 (peptidoglycan-based cell wall)