Protein: Farnesyl pyrophosphate synthase (A0A1D8PH78)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Farnesyl pyrophosphate synthase; part of the second module of ergosterol biosynthesis pathway that includes the middle steps of the pathway. ERG20 catalyzes the sequential condensation of isopentenyl pyrophosphate with dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate. The second module is carried out in the vacuole and involves the formation of farnesyl diphosphate, which is also an important intermediate in the biosynthesis of ubiquinone, dolichol, heme and prenylated proteins. Activity by the mevalonate kinase ERG12 first converts mevalonate into 5- phosphomevalonate. 5-phosphomevalonate is then further converted to 5- diphosphomevalonate by the phosphomevalonate kinase ERG8. The diphosphomevalonate decarboxylase MVD then produces isopentenyl diphosphate. The isopentenyl-diphosphate delta-isomerase IDI1 then catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP). Finally the farnesyl diphosphate synthase ERG20 catalyzes the sequential condensation of isopentenyl pyrophosphate with dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate (Probable)
Sequence: MSDKLAARERFLDVFEDLVEELKQILVSYNMPQEAIEWFVRSLNYNTPGGKLNRGLSVVDTFAILNNTTSDKLNDTEYKKVALLGWAIELLQAYFLVADDMMDQSKTRRGQPCWYLVEGVNNIAINDSFMLEGAIYILLKKHFRQDPYYVDLLDLFHEVTFQTELGQLLDLITADEEIVDLDKFSLEKHSFIVIFKTAYYSFYLPVALAMYMSGINDEKDLKQVRDILIPLGEYFQIQDDYLDCFGTPEQIGKIGTDIKDNKCSWVINQALLIATPEQRQLLDNNYGKKDDESEQKCKDLFKQLGIEKIYHDYEESIVAKLRKQIDQIDESRGLKKDVLTAFLGKVYKRSK
InterPro domains: - IPR000092: Polyprenyl synthetase-like (family) [34-349]
- IPR033749: Polyprenyl synthetase, conserved site (conserved_site) [96-243]
- IPR039702: Farnesyl pyrophosphate synthase-like (family) [7-351]
- IPR008949: Isoprenoid synthase domain superfamily (homologous_superfamily) [2-350]
Interaction partners: - Isopentenyl-diphosphate delta-isomerase
- Diphosphomevalonate decarboxylase
- Squalene synthase ERG9
- Farnesyltranstransferase
- Trans-hexaprenyltranstransferase
- Alkyl transferase
- Alkyl transferase
- Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha
- Mevalonate kinase
- Protein farnesyltransferase subunit beta
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016765 (transferase activity, transferring alkyl or aryl (other than methyl) groups), GO:0004659 (prenyltransferase activity), GO:0004311 (farnesyltranstransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0019637 (organophosphate metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006066 (alcohol metabolic process), GO:0044107 (cellular alcohol metabolic process), GO:0006793 (phosphorus metabolic process), GO:0016125 (sterol metabolic process), GO:1902652 (secondary alcohol metabolic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0008204 (ergosterol metabolic process), GO:0006720 (isoprenoid metabolic process), GO:0006644 (phospholipid metabolic process), GO:0008202 (steroid metabolic process), GO:0097384 (cellular lipid biosynthetic process), GO:0090407 (organophosphate biosynthetic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0044108 (cellular alcohol biosynthetic process), GO:0008299 (isoprenoid biosynthetic process), GO:0046165 (alcohol biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0016128 (phytosteroid metabolic process), GO:0006696 (ergosterol biosynthetic process), GO:0008654 (phospholipid biosynthetic process), GO:0006694 (steroid biosynthetic process), GO:0045338 (farnesyl diphosphate metabolic process), GO:0016126 (sterol biosynthetic process), GO:0016129 (phytosteroid biosynthetic process), GO:1902653 (secondary alcohol biosynthetic process), GO:0006721 (terpenoid metabolic process), GO:0016114 (terpenoid biosynthetic process), GO:0045337 (farnesyl diphosphate biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)