Protein: Transcriptional activator/repressor GIS1 (Q03833)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Transcription factor involved in the regulation of gene expression upon nutrient starvation. Recognizes and binds to the post-diauxic-shift element 5'-T[AT]AGGGAT-3' in the promoter region. Can act as a transcriptional activator (e.g. of stress genes like SSA3, HSP12 and HSP26) as well as a repressor (e.g. of pyrophosphate phosphatase DPP1). GIS1 also acts as a DNA damage-responsive transcriptional repressor of photolyase PHR1.
Subcellular location: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00537, ECO:0000269|PubMed:14562095}.
Sequence: MEIKPVEVIDGVPVFKPSMMEFANFQYFIDEITKFGIENGIVKVIPPKEWLELLEGSPPAESLKTIQLDSPIQQQAKRWDKHENGVFSIENEYDNKSYNLTQWKNLAESLDSRISQGDFNDKTLKENCRVDSQQDCYDLAQLQILESDFWKTIAFSKPFYAVDENSSIFPYDLTLWNLNNLPDSINSSNRRLLTGQSKCIFPWHLDEQNKCSINYLHFGAPKQWYSIPSANTDQFLKILSKEPSSNKENCPAFIRHQNIITSPDFLRKNNIKFNRVVQFQHEFIITFPYCMYSGFNYGYNFGESIEFILDQQAVVRKQPLKCGCGNKKEERKSGPFSNLSYDSNESEQRGSITDNDNDLFQKVRSFDELLNHSSQELQNLEDNKNPLFSNINMNRPQSSSLRSTTPNGVNQFLNMNQTTISRISSPLLSRMMDLSNIVEPTLDDPGSKFKRKVLTPQLPQMNIPSNSSNFGTPSLTNTNSLLSNITATSTNPSTTTNGSQNHNNVNANGINTSAAASINNNISSTNNSANNSSSNNNVSTVPSSMMHSSTLNGTSGLGGDNDDNMLALSLATLANSATASPRLTLPPLSSPMNPNGHTSYNGNMMNNNSGNGSNGSNSYSNGVTTAAATTTSAPHNLSIVSPNPTYSPNPLSLYLTNSKNPLNSGLAPLSPSTSNIPFLKRNNVVTLNISREASKSPISSFVNDYRSPLGVSNPLMYSSTINDYSNGTGIRQNSNNINPLDAGPSFSPLHKKPKILNGNDNSNLDSNNFDYSFTGNKQESNPSILNNNTNNNDNYRTSSMNNNGNNYQAHSSKFGENEVIMSDHGKIYICRECNRQFSSGHHLTRHKKSVHSGEKPHSCPRCGKRFKRRDHVLQHLNKKIPCTQEMENTKLAES
InterPro domains: - IPR003347: JmjC domain (domain) [170-324]
- IPR013087: Zinc finger C2H2-type (domain) [828-889]
- IPR036236: Zinc finger C2H2 superfamily (homologous_superfamily) [826-876]
- IPR003349: JmjN domain (domain) [11-53]
Interaction partners: - Serine/threonine-protein kinase SCH9
- Ras-like protein 2
- Zinc finger protein MSN2
- Serine/threonine-protein kinase RIM15
- Zinc finger protein MSN4
- JmjC domain-containing histone demethylation protein 1
- Protein ECM5
- Histone H3
- DNA damage-responsive transcriptional repressor RPH1
- Serine/threonine-protein kinase TOR1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140110 (transcription regulator activity), GO:0032451 (demethylase activity), GO:0003700 (DNA-binding transcription factor activity), GO:1901363 (heterocyclic compound binding), GO:0097159 (organic cyclic compound binding), GO:0140096 (catalytic activity, acting on a protein), GO:0016491 (oxidoreductase activity), GO:0003676 (nucleic acid binding), GO:0051213 (dioxygenase activity), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0001217 (DNA-binding transcription repressor activity), GO:0000981 (DNA-binding transcription factor activity, RNA polymerase II-specific), GO:0140457 (protein demethylase activity), GO:0001227 (DNA-binding transcription repressor activity, RNA polymerase II-specific), GO:0032452 (histone demethylase activity), GO:0003677 (DNA binding), GO:0016706 (2-oxoglutarate-dependent dioxygenase activity), GO:0043565 (sequence-specific DNA binding), GO:0032454 (histone H3K9 demethylase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0071704 (organic substance metabolic process), GO:0048523 (negative regulation of cellular process), GO:0044238 (primary metabolic process), GO:0070988 (demethylation), GO:0009892 (negative regulation of metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006482 (protein demethylation), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0019538 (protein metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0016577 (histone demethylation), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0051253 (negative regulation of RNA metabolic process), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0010468 (regulation of gene expression), GO:0070076 (histone lysine demethylation), GO:0008214 (protein dealkylation), GO:2001141 (regulation of RNA biosynthetic process), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0016570 (histone modification), GO:0006357 (regulation of transcription by RNA polymerase II), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0016575 (histone deacetylation), GO:0006476 (protein deacetylation), GO:0045892 (negative regulation of DNA-templated transcription), GO:0000122 (negative regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)