Protein: Phosphoglucomutase 2 (PGM 2) (EC 5.4.2.2) (D-glucose-1,6-diphosphate:D-glucose-1-phosphate phosphotransferase) (Glucose phosphomutase 2) (P37012)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Major phosphoglucomutase isozyme that catalyzes the reversible interconversion of glucose 1-phosphate and glucose 6-phosphate. Constitutes about 80-90% of the phosphoglucomutase activity in the cell. Key enzyme in hexose metabolism. The forward reaction is an essential step in the energy metabolism of galactose since the product of the galactose pathway enzymes in yeast is glucose 1-phosphate. The reverse reaction is an essential step for biosynthesis when carbon sources other than galactose are the energy source because glucose 1-phosphate is the starting point for the synthesis of UDP-glucose, which acts as a precursor for the synthesis of oligosaccharides and trehalose.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:8385141}.
Sequence: MSFQIETVPTKPYEDQKPGTSGLRKKTKVFKDEPNYTENFIQSIMEAIPEGSKGATLVVGGDGRYYNDVILHKIAAIGAANGIKKLVIGQHGLLSTPAASHIMRTYEEKCTGGIILTASHNPGGPENDMGIKYNLSNGGPAPESVTNAIWEISKKLTSYKIIKDFPELDLGTIGKNKKYGPLLVDIIDITKDYVNFLKEIFDFDLIKKFIDNQRSTKNWKLLFDSMNGVTGPYGKAIFVDEFGLPADEVLQNWHPSPDFGGMHPDPNLTYASSLVKRVDREKIEFGAASDGDGDRNMIYGYGPSFVSPGDSVAIIAEYAAEIPYFAKQGIYGLARSFPTSGAIDRVAKAHGLNCYEVPTGWKFFCALFDAKKLSICGEESFGTGSNHVREKDGVWAIMAWLNILAIYNKHHPENEASIKTIQNEFWAKYGRTFFTRYDFEKVETEKANKIVDQLRAYVTKSGVVNSAFPADESLKVTDCGDFSYTDLDGSVSDHQGLYVKLSNGARFVLRLSGTGSSGATIRLYIEKYCDDKSQYQKTAEEYLKPIINSVIKFLNFKQVLGTEEPTVRT
InterPro domains: - IPR036900: Alpha-D-phosphohexomutase, C-terminal domain superfamily (homologous_superfamily) [431-569]
- IPR005846: Alpha-D-phosphohexomutase, alpha/beta/alpha domain III (domain) [312-429]
- IPR016066: Alpha-D-phosphohexomutase, conserved site (conserved_site) [113-122]
- IPR005845: Alpha-D-phosphohexomutase, alpha/beta/alpha domain II (domain) [202-299]
- IPR016055: Alpha-D-phosphohexomutase, alpha/beta/alpha I/II/III (homologous_superfamily) [3-436]
- IPR005841: Alpha-D-phosphohexomutase superfamily (family) [112-298]
- IPR045244: Phosphoglucomutase (family) [3-569]
- IPR005844: Alpha-D-phosphohexomutase, alpha/beta/alpha domain I (domain) [16-159]
Interaction partners: - UTP--glucose-1-phosphate uridylyltransferase
- Glycogen phosphorylase
- Phosphoribomutase
- Glucose-6-phosphate isomerase
- Hexokinase-1
- Glucokinase-1
- Glycogen debranching enzyme
- Phosphoglucomutase 1
- Glycogen [starch] synthase isoform 2
- Probable UTP--glucose-1-phosphate uridylyltransferase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016853 (isomerase activity), GO:0016866 (intramolecular transferase activity), GO:0016868 (intramolecular transferase activity, phosphotransferases)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044282 (small molecule catabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044249 (cellular biosynthetic process), GO:0005996 (monosaccharide metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0005975 (carbohydrate metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0006091 (generation of precursor metabolites and energy), GO:0006793 (phosphorus metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0019318 (hexose metabolic process), GO:0046365 (monosaccharide catabolic process), GO:0009311 (oligosaccharide metabolic process), GO:0044264 (cellular polysaccharide metabolic process), GO:0016051 (carbohydrate biosynthetic process), GO:0005976 (polysaccharide metabolic process), GO:0034637 (cellular carbohydrate biosynthetic process), GO:0009225 (nucleotide-sugar metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0015980 (energy derivation by oxidation of organic compounds), GO:0016052 (carbohydrate catabolic process), GO:0006011 (UDP-glucose metabolic process), GO:0051156 (glucose 6-phosphate metabolic process), GO:0005984 (disaccharide metabolic process), GO:0005991 (trehalose metabolic process), GO:0019320 (hexose catabolic process), GO:0009312 (oligosaccharide biosynthetic process), GO:0033692 (cellular polysaccharide biosynthetic process), GO:0044042 (glucan metabolic process), GO:0006112 (energy reserve metabolic process), GO:0000271 (polysaccharide biosynthetic process), GO:0006012 (galactose metabolic process), GO:0046351 (disaccharide biosynthetic process), GO:0006073 (cellular glucan metabolic process), GO:0009250 (glucan biosynthetic process), GO:0005992 (trehalose biosynthetic process), GO:0005977 (glycogen metabolic process), GO:0019388 (galactose catabolic process), GO:0005978 (glycogen biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)