Protein: Matrix metalloproteinase-16 (MMP-16) (EC 3.4.24.-) (MMP-X2) (Membrane-type matrix metalloproteinase 3) (MT-MMP 3) (MTMMP3) (Membrane-type-3 matrix metalloproteinase) (MT3-MMP) (MT3MMP) (P51512)
Organism: Homo sapiens (Human)
Function: Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
Subcellular location: [Isoform Long]: Cell membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}; Extracellular side {ECO:0000305}. Note=Localized at the cell surface of melanoma cells.; SUBCELLULAR LOCATION: [Isoform Short]: Secreted, extracellular space, extracellular matrix. Cell surface. Note=Localized at the cell surface of melanoma cells.
Sequence: MILLTFSTGRRLDFVHHSGVFFLQTLLWILCATVCGTEQYFNVEVWLQKYGYLPPTDPRMSVLRSAETMQSALAAMQQFYGINMTGKVDRNTIDWMKKPRCGVPDQTRGSSKFHIRRKRYALTGQKWQHKHITYSIKNVTPKVGDPETRKAIRRAFDVWQNVTPLTFEEVPYSELENGKRDVDITIIFASGFHGDSSPFDGEGGFLAHAYFPGPGIGGDTHFDSDEPWTLGNPNHDGNDLFLVAVHELGHALGLEHSNDPTAIMAPFYQYMETDNFKLPNDDLQGIQKIYGPPDKIPPPTRPLPTVPPHRSIPPADPRKNDRPKPPRPPTGRPSYPGAKPNICDGNFNTLAILRREMFVFKDQWFWRVRNNRVMDGYPMQITYFWRGLPPSIDAVYENSDGNFVFFKGNKYWVFKDTTLQPGYPHDLITLGSGIPPHGIDSAIWWEDVGKTYFFKGDRYWRYSEEMKTMDPGYPKPITVWKGIPESPQGAFVHKENGFTYFYKGKEYWKFNNQILKVEPGYPRSILKDFMGCDGPTDRVKEGHSPPDDVDIVIKLDNTASTVKAIAIVIPCILALCLLVLVYTVFQFKRKGTPRHILYCKRSMQEWV
InterPro domains: - IPR036365: PGBD-like superfamily (homologous_superfamily) [29-106]
- IPR000585: Hemopexin-like domain (domain) [340-532]
- IPR018486: Hemopexin, conserved site (conserved_site) [381-396]
- IPR021158: Peptidase M10A, cysteine switch, zinc binding site (binding_site) [99-106]
- IPR021805: Peptidase M10A, matrix metallopeptidase, C-terminal (domain) [538-607]
- IPR006026: Peptidase, metallopeptidase (domain) [123-292]
- IPR002477: Peptidoglycan binding-like (domain) [43-96]
- IPR021190: Peptidase M10A (family) [15-550]
- IPR024079: Metallopeptidase, catalytic domain superfamily (homologous_superfamily) [33-298]
- IPR001818: Peptidase M10, metallopeptidase (domain) [126-291]
- IPR018487: Hemopexin-like repeats (repeat) [340-532]
- IPR033739: Peptidase M10A, catalytic domain (domain) [126-291]
- IPR036375: Hemopexin-like domain superfamily (homologous_superfamily) [336-534]
Interaction partners: - Proheparin-binding EGF-like growth factor
- Metalloproteinase inhibitor 2
- Guanine nucleotide-binding protein G
- Guanine nucleotide-binding protein G
- Guanine nucleotide-binding protein G
- Testican-3
- Testican-2
- Testican-1
- Collagenase 3
- Metalloproteinase inhibitor 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0043167 (ion binding), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0008233 (peptidase activity), GO:0017171 (serine hydrolase activity), GO:0043169 (cation binding), GO:0046872 (metal ion binding), GO:0008237 (metallopeptidase activity), GO:0008236 (serine-type peptidase activity), GO:0004175 (endopeptidase activity), GO:0008238 (exopeptidase activity), GO:0004222 (metalloendopeptidase activity), GO:0004177 (aminopeptidase activity), GO:0008235 (metalloexopeptidase activity), GO:0046914 (transition metal ion binding), GO:0004252 (serine-type endopeptidase activity), GO:0070006 (metalloaminopeptidase activity), GO:0008270 (zinc ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0048870 (cell motility), GO:0006807 (nitrogen compound metabolic process), GO:0001503 (ossification), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0032963 (collagen metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0009887 (animal organ morphogenesis), GO:1901564 (organonitrogen compound metabolic process), GO:0036075 (replacement ossification), GO:0009888 (tissue development), GO:0048731 (system development), GO:0019538 (protein metabolic process), GO:0048513 (animal organ development), GO:0030574 (collagen catabolic process), GO:0016043 (cellular component organization), GO:0043170 (macromolecule metabolic process), GO:0016477 (cell migration), GO:0051216 (cartilage development), GO:0048705 (skeletal system morphogenesis), GO:0060348 (bone development), GO:0006508 (proteolysis), GO:0001501 (skeletal system development), GO:0043062 (extracellular structure organization), GO:0001958 (endochondral ossification), GO:0061448 (connective tissue development), GO:0060349 (bone morphogenesis), GO:0045229 (external encapsulating structure organization), GO:0022411 (cellular component disassembly), GO:0022617 (extracellular matrix disassembly), GO:0030198 (extracellular matrix organization), GO:0060350 (endochondral bone morphogenesis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0031974 (membrane-enclosed lumen), GO:0005829 (cytosol), GO:0071944 (cell periphery), GO:0030054 (cell junction), GO:0005615 (extracellular space), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005576 (extracellular region), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043228 (non-membrane-bounded organelle), GO:0005794 (Golgi apparatus), GO:0043233 (organelle lumen), GO:0070161 (anchoring junction), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0030055 (cell-substrate junction), GO:0043231 (intracellular membrane-bounded organelle), GO:0044352 (pinosome), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005796 (Golgi lumen), GO:0031982 (vesicle), GO:0070013 (intracellular organelle lumen), GO:0005925 (focal adhesion), GO:0097708 (intracellular vesicle), GO:0044354 (macropinosome), GO:0005634 (nucleus), GO:0005856 (cytoskeleton), GO:0045111 (intermediate filament cytoskeleton)