Protein: DNA-directed RNA polymerases I and III subunit RPAC1 (RNA polymerases I and III subunit AC1) (C37) (DNA-directed RNA polymerases I and III 40 kDa polypeptide) (AC40) (C40) (P07703)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I (Pol I) and III (Pol III) which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPC40 is part of the polymerase core and may function as a clamp element that moves to open and close the cleft. Plays an important role in targeting retrotransposons Ty integration upstream of pol III-transcribed genes such as tRNA genes, allowing Ty1, Ty2 and Ty4 to proliferate and yet minimizing genetic damage.
Subcellular location: Nucleus, nucleolus {ECO:0000269|PubMed:14562095}.
Sequence: MSNIVGIEYNRVTNTTSTDFPGFSKDAENEWNVEKFKKDFEVNISSLDAREANFDLINIDTSIANAFRRIMISEVPSVAAEYVYFFNNTSVIQDEVLAHRIGLVPLKVDPDMLTWVDSNLPDDEKFTDENTIVLSLNVKCTRNPDAPKGSTDPKELYNNAHVYARDLKFEPQGRQSTTFADCPVVPADPDILLAKLRPGQEISLKAHCILGIGGDHAKFSPVSTASYRLLPQINILQPIKGESARRFQKCFPPGVIGIDEGSDEAYVKDARKDTVSREVLRYEEFADKVKLGRVRNHFIFNVESAGAMTPEEIFFKSVRILKNKAEYLKNCPITQ
InterPro domains: - IPR001514: DNA-directed RNA polymerase, 30-40kDa subunit, conserved site (conserved_site) [65-105]
- IPR036603: RNA polymerase, RBP11-like subunit (homologous_superfamily) [38-331]
- IPR011263: DNA-directed RNA polymerase, RpoA/D/Rpb3-type (domain) [51-331]
- IPR011262: DNA-directed RNA polymerase, insert domain (domain) [89-218]
- IPR050518: Archaeal Rpo3/Eukaryotic RPB3 RNA Polymerase Subunit (family) [5-328]
- IPR022842: DNA-directed RNA polymerase subunit Rpo3/Rpb3/RPAC1 (family) [41-332]
- IPR036643: DNA-directed RNA polymerase, insert domain superfamily (homologous_superfamily) [76-221]
- IPR033901: DNA-directed RNA polymerases I and III subunit AC40 (family) [40-330]
Interaction partners: - DNA-directed RNA polymerase I subunit RPA12
- DNA-directed RNA polymerase I subunit RPA34
- DNA-directed RNA polymerase III subunit RPC8
- DNA-directed RNA polymerases I, II, and III subunit RPABC3
- DNA-directed RNA polymerases I, II, and III subunit RPABC1
- DNA-directed RNA polymerase III subunit RPC6
- DNA-directed RNA polymerases I, II, and III subunit RPABC4
- DNA-directed RNA polymerase III subunit RPC7
- DNA-directed RNA polymerase I subunit RPA43
- DNA-directed RNA polymerase I subunit RPA49
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0005515 (protein binding), GO:0140098 (catalytic activity, acting on RNA), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0097747 (RNA polymerase activity), GO:0016779 (nucleotidyltransferase activity), GO:0034062 (5'-3' RNA polymerase activity), GO:0003899 (DNA-directed 5'-3' RNA polymerase activity), GO:0003968 (RNA-dependent RNA polymerase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0010467 (gene expression), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0016070 (RNA metabolic process), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006352 (DNA-templated transcription initiation), GO:0006351 (DNA-templated transcription), GO:0006353 (DNA-templated transcription termination), GO:0006354 (DNA-templated transcription elongation), GO:0034660 (ncRNA metabolic process), GO:0006360 (transcription by RNA polymerase I), GO:0006361 (transcription initiation at RNA polymerase I promoter), GO:0006363 (termination of RNA polymerase I transcription), GO:0016072 (rRNA metabolic process), GO:0098781 (ncRNA transcription), GO:0006362 (transcription elongation by RNA polymerase I), GO:0042790 (nucleolar large rRNA transcription by RNA polymerase I), GO:0009303 (rRNA transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0140535 (intracellular protein-containing complex), GO:1902494 (catalytic complex), GO:0140513 (nuclear protein-containing complex), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0030880 (RNA polymerase complex), GO:1990234 (transferase complex), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043233 (organelle lumen), GO:0055029 (nuclear DNA-directed RNA polymerase complex), GO:0043227 (membrane-bounded organelle), GO:0000428 (DNA-directed RNA polymerase complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0061695 (transferase complex, transferring phosphorus-containing groups), GO:0005666 (RNA polymerase III complex), GO:0070013 (intracellular organelle lumen), GO:0005736 (RNA polymerase I complex), GO:0005730 (nucleolus), GO:0005634 (nucleus), GO:0031981 (nuclear lumen)