Protein: Tyrosine--tRNA ligase, cytoplasmic (EC 6.1.1.1) (Tyrosyl-tRNA synthetase) (TyrRS) (O14055)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
Subcellular location: Cytoplasm.
Sequence: MSLTPDEKYELITRNLQEVLGAKMIREILNERDISLYWGSAPTGRPHCGYFVPMMKLADFLKAGVHVTVLLADVHAFLDNMKAPMELVQHRVRYYEIIVKSILKSLNIPIEKLRFVIGSSYQLKEDYTLDNFRLAASTTEHTARRAGAEVVKQVASPLLSSLIYPGMQALDEQYLGVDVQFGGVDQRKIFVMAEEVLPVLGYKKRGHLMNPMVPSLAGGKMSSSAAANAKIDILDDPKTVNKKIRSAFCEPGVVEENGCLAFLKYVSFPAMELRGETEFVIDRPEQFGGRMVFKTYEEVEKAYKDLTLSPQDLKLGLESSVNTLLAGVQEQLKQYPDLDSILKAAYPDPKDLKKAMKQKKQDKKNAKKAGTTNASIAAESGAAPATESQTAESFKKLNLDD
InterPro domains: - IPR002305: Aminoacyl-tRNA synthetase, class Ic (family) [30-325]
- IPR002307: Tyrosine-tRNA ligase (family) [7-332]
- IPR050489: Tyrosine--tRNA ligase (family) [3-346]
- IPR014729: Rossmann-like alpha/beta/alpha sandwich fold (homologous_superfamily) [1-211]
- IPR023617: Tyrosine-tRNA ligase, archaeal/eukaryotic-type (family) [3-351]
Interaction partners: - Putative leucine--tRNA ligase, cytoplasmic
- tRNA-aminoacylation cofactor arc1
- Putative leucine--tRNA ligase, mitochondrial
- Isoleucine--tRNA ligase, mitochondrial
- Isoleucine--tRNA ligase, cytoplasmic
- Tyrosine--tRNA ligase, mitochondrial
- Probable cysteine--tRNA ligase
- Tryptophan--tRNA ligase, cytoplasmic
- Putative glycine--tRNA ligase
- Tryptophan--tRNA ligase, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0140098 (catalytic activity, acting on RNA), GO:0016875 (ligase activity, forming carbon-oxygen bonds), GO:0004812 (aminoacyl-tRNA ligase activity), GO:0140101 (catalytic activity, acting on a tRNA)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006082 (organic acid metabolic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006412 (translation), GO:0009059 (macromolecule biosynthetic process), GO:0006518 (peptide metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0043038 (amino acid activation), GO:0010467 (gene expression), GO:0019752 (carboxylic acid metabolic process), GO:0016070 (RNA metabolic process), GO:0043043 (peptide biosynthetic process), GO:0006418 (tRNA aminoacylation for protein translation), GO:0043039 (tRNA aminoacylation), GO:0034660 (ncRNA metabolic process), GO:0006399 (tRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)