Protein: DNA-directed RNA polymerase subunit Rpo3 (EC 2.7.7.6) (DNA-directed RNA polymerase subunit D) (P39471)
Organism: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Function: DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00320}.
Sequence: MPISLIERNGLRLRLVLENYPLEFVNSIRRASILYVPVMAVDEVYFIENNSPLYDEILAHRLALVPFVSDEALEHYRPPEECAECKENCDGCYNRVYLDVEAKDQPLMIYSRDLKSEDQMITPVSGAIPIVLLGSKQKISLEARLRLGYGKEHIKYSPVSVSIVRYYPKVTVLGNCEKAVEVCPEGVFAMENNKLVVKNELSCILCEECLKYCAGSVSIESVENKFILEIESVGSLKPERILIEASKSLLRKLSELKSKLEAGK
InterPro domains: - IPR001514: DNA-directed RNA polymerase, 30-40kDa subunit, conserved site (conserved_site) [26-66]
- IPR036603: RNA polymerase, RBP11-like subunit (homologous_superfamily) [3-261]
- IPR011263: DNA-directed RNA polymerase, RpoA/D/Rpb3-type (domain) [12-259]
- IPR011262: DNA-directed RNA polymerase, insert domain (domain) [50-155]
- IPR050518: Archaeal Rpo3/Eukaryotic RPB3 RNA Polymerase Subunit (family) [12-261]
- IPR022842: DNA-directed RNA polymerase subunit Rpo3/Rpb3/RPAC1 (family) [2-260]
- IPR036643: DNA-directed RNA polymerase, insert domain superfamily (homologous_superfamily) [37-158]
Interaction partners: - Small ribosomal subunit protein uS17
- DNA-directed RNA polymerase subunit Rpo1C
- Large ribosomal subunit protein uL18
- Small ribosomal subunit protein uS19
- Large ribosomal subunit protein uL13
- Large ribosomal subunit protein uL24
- Small ribosomal subunit protein uS2
- DNA-directed RNA polymerase subunit Rpo11
- Large ribosomal subunit protein uL15
- Large ribosomal subunit protein uL3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0016740 (transferase activity), GO:0140098 (catalytic activity, acting on RNA), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0097747 (RNA polymerase activity), GO:0016779 (nucleotidyltransferase activity), GO:0034062 (5'-3' RNA polymerase activity), GO:0003899 (DNA-directed 5'-3' RNA polymerase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0010467 (gene expression), GO:0018130 (heterocycle biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0016070 (RNA metabolic process), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006351 (DNA-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0140535 (intracellular protein-containing complex), GO:0030880 (RNA polymerase complex), GO:1990234 (transferase complex), GO:0000428 (DNA-directed RNA polymerase complex), GO:0061695 (transferase complex, transferring phosphorus-containing groups)