Protein: Actin-related protein 2/3 complex subunit 4 (Arp2/3 complex 20 kDa subunit) (p20-ARC) (P59999)
Organism: Mus musculus (Mouse)
Function: Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
Subcellular location: Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P59998}. Cell projection {ECO:0000250|UniProtKB:P59998}. Nucleus {ECO:0000250|UniProtKB:P59998}.
Sequence: MTATLRPYLSAVRATLQAALCLENFSSQVVERHNKPEVEVRSSKELLLQPVTISRNEKEKVLIEGSINSVRVSIAVKQADEIEKILCHKFMRFMMMRAENFFILRRKPVEGYDISFLITNFHTEQMYKHKLVDFVIHFMEEIDKEISEMKLSVNARARIVAEEFLKNF
InterPro domains: - IPR034666: Arp2/3 complex subunit 2/4 (homologous_superfamily) [1-168]
- IPR008384: Actin-related protein 2/3 complex subunit 4 (family) [1-168]
Interaction partners: - Actin-related protein 2/3 complex subunit 2
- Actin-related protein 2
- Actin-related protein 2/3 complex subunit 3
- Actin-related protein 2/3 complex subunit 5
- Actin-related protein 2/3 complex subunit 1B
- Actin-related protein 2/3 complex subunit 5-like protein
- Actin-related protein 3
- Actin-related protein 2/3 complex subunit 1A
- Actin nucleation-promoting factor WASL
- Actin nucleation-promoting factor WAS
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0044877 (protein-containing complex binding), GO:0005515 (protein binding), GO:0051015 (actin filament binding), GO:0008092 (cytoskeletal protein binding), GO:0003779 (actin binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0030029 (actin filament-based process), GO:0030036 (actin cytoskeleton organization), GO:0016043 (cellular component organization), GO:0006996 (organelle organization), GO:0097435 (supramolecular fiber organization), GO:0007015 (actin filament organization), GO:0007010 (cytoskeleton organization), GO:0045010 (actin nucleation), GO:0034314 (Arp2/3 complex-mediated actin nucleation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005885 (Arp2/3 protein complex), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0005885 (Arp2/3 protein complex), GO:0043229 (intracellular organelle), GO:0005938 (cell cortex), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0030863 (cortical cytoskeleton), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0030864 (cortical actin cytoskeleton), GO:0005634 (nucleus), GO:0015629 (actin cytoskeleton)