Protein: Calnexin (P35565)
Organism: Rattus norvegicus (Rat)
Function: Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P24643}; Single-pass type I membrane protein {ECO:0000255}. Mitochondrion membrane {ECO:0000250|UniProtKB:P24643}; Single-pass type I membrane protein {ECO:0000255}. Melanosome membrane {ECO:0000250|UniProtKB:P27824}; Single-pass type I membrane protein {ECO:0000255}. Note=The palmitoylated form preferentially localizes to the perinuclear rough ER (By similarity). Localizes to endoplasmic reticulum mitochondria-associated membrane (MAMs) that connect the endoplasmic reticulum and the mitochondria (By similarity). {ECO:0000250|UniProtKB:P24643, ECO:0000250|UniProtKB:P27824}.
Sequence: MEGKWLLCLLLVLGTAAIQAHDGHDDDMIDIEDDLDDVIEEVEDSKSKSDTSTPPSPKVTYKAPVPTGEVYFADSFDRGSLSGWILSKAKKDDTDDEIAKYDGKWEVDEMKETKLPGDKGLVLMSRAKHHAISAKLNKPFLFDTKPLIVQYEVNFQNGIECGGAYVKLLSKTSELNLDQFHDKTPYTIMFGPDKCGEDYKLHFIFRHKNPKTGVYEEKHAKRPDADLKTYFTDKKTHLYTLILNPDNSFEILVDQSVVNSGNLLNDMTPPVNPSREIEDPEDRKPEDWDERPKIADPDAVKPDDWDEDAPSKIPDEEATKPEGWLDDEPEYIPDPDAEKPEDWDEDMDGEWEAPQIANPKCESAPGCGVWQRPMIDNPNYKGKWKPPMIDNPNYQGIWKPRKIPNPDFFEDLEPFRMTPFSAIGLELWSMTSDIFFDNFIISGDRRVVDDWANDGWGLKKAADGAAEPGVVGQMLEAAEERPWLWVVYILTVALPVFLVILFCCSGKKQSNAMEYKKTDAPQPDVKDEEGKEEEKNKGDEEEEEEKLEEKQKSDAEEDGGTGSQDEEDSKPKAEEDEILNRSPRNRKPRRE
InterPro domains: - IPR013320: Concanavalin A-like lectin/glucanase domain superfamily (homologous_superfamily) [61-457]
- IPR009033: Calreticulin/calnexin, P domain superfamily (homologous_superfamily) [270-415]
- IPR001580: Calreticulin/calnexin (family) [63-544]
- IPR018124: Calreticulin/calnexin, conserved site (conserved_site) [154-344]
Interaction partners: - Protein disulfide-isomerase A3
- Calreticulin
- Calreticulin
- Calreticulin
- TAP binding protein
- Endoplasmic reticulum chaperone BiP
- Endoplasmin
- UDP-glucose:glycoprotein glucosyltransferase 1
- Translocon-associated protein subunit alpha
- Beta-2-microglobulin
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0051179 (localization), GO:0051234 (establishment of localization), GO:0051641 (cellular localization), GO:0051649 (establishment of localization in cell), GO:0006810 (transport), GO:0036465 (synaptic vesicle recycling), GO:0099504 (synaptic vesicle cycle), GO:0016192 (vesicle-mediated transport), GO:0140238 (presynaptic endocytosis), GO:0048488 (synaptic vesicle endocytosis), GO:0006897 (endocytosis), GO:0099003 (vesicle-mediated transport in synapse), GO:0006898 (receptor-mediated endocytosis), GO:0072583 (clathrin-dependent endocytosis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0098552 (side of membrane), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0031984 (organelle subcompartment), GO:0043209 (myelin sheath), GO:0031975 (envelope), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0031967 (organelle envelope), GO:0043229 (intracellular organelle), GO:0098576 (lumenal side of membrane), GO:0098827 (endoplasmic reticulum subcompartment), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0005783 (endoplasmic reticulum), GO:0005635 (nuclear envelope), GO:0043227 (membrane-bounded organelle), GO:0031965 (nuclear membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0098553 (lumenal side of endoplasmic reticulum membrane), GO:0005789 (endoplasmic reticulum membrane), GO:0005634 (nucleus)