Protein: Beclin 1-associated autophagy-related key regulator (Barkor) (Autophagy-related protein 14-like protein) (Atg14L) (Q6ZNE5)
Organism: Homo sapiens (Human)
Function: Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1. Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK. Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion. Modulates the hepatic lipid metabolism (By similarity).
Subcellular location: Cytoplasm {ECO:0000269|PubMed:19050071, ECO:0000269|PubMed:37632749}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:19270696, ECO:0000269|PubMed:20713597}; Peripheral membrane protein {ECO:0000305}. Preautophagosomal structure membrane {ECO:0000269|PubMed:18843052, ECO:0000269|PubMed:19050071, ECO:0000269|PubMed:19270696, ECO:0000269|PubMed:22314358}; Peripheral membrane protein {ECO:0000305}. Cytoplasmic vesicle, autophagosome membrane {ECO:0000269|PubMed:19270696, ECO:0000269|PubMed:25686604}; Peripheral membrane protein {ECO:0000305}. Note=Cytosolic under nutrient-rich conditions (PubMed:19050071). Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes (PubMed:19050071). Accumulates on highly curved PtdIns(3)P enriched autophagic membrane via its BATS domain to sense and maintain membrane curvature (By similarity). Also localizes to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity). {ECO:0000250|UniProtKB:Q8CDJ3}.
Sequence: MASPSGKGARALEAPGCGPRPLARDLVDSVDDAEGLYVAVERCPLCNTTRRRLTCAKCVQSGDFVYFDGRDRERFIDKKERLSRLKSKQEEFQKEVLKAMEGKWITDQLRWKIMSCKMRIEQLKQTICKGNEEMEKNSEGLLKTKEKNQKLYSRAQRHQEKKEKIQRHNRKLGDLVEKKTIDLRSHYERLANLRRSHILELTSVIFPIEEVKTGVRDPADVSSESDSAMTSSTVSKLAEARRTTYLSGRWVCDDHNGDTSISITGPWISLPNNGDYSAYYSWVEEKKTTQGPDMEQSNPAYTISAALCYATQLVNILSHILDVNLPKKLCNSEFCGENLSKQKFTRAVKKLNANILYLCFSQHVNLDQLQPLHTLRNLMYLVSPSSEHLGRSGPFEVRADLEESMEFVDPGVAGESDESGDERVSDEETDLGTDWENLPSPRFCDIPSQSVEVSQSQSTQASPPIASSSAGGMISSAAASVTSWFKAYTGHR
InterPro domains: - IPR018791: UV radiation resistance protein/autophagy-related protein 14 (family) [43-336]
Interaction partners: - RB1-inducible coiled-coil protein 1
- Synaptosomal-associated protein 29
- Beclin-2
- Syntaxin-17
- Phosphoinositide 3-kinase regulatory subunit 4
- Nuclear receptor-binding factor 2
- Serine/threonine-protein kinase ULK1
- Phosphatidylinositol 3-kinase catalytic subunit type 3
- UV radiation resistance-associated gene protein
- Beclin-1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0048522 (positive regulation of cellular process), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0051716 (cellular response to stimulus), GO:0071840 (cellular component organization or biogenesis), GO:0006950 (response to stress), GO:0061919 (process utilizing autophagic mechanism), GO:0009056 (catabolic process), GO:0044085 (cellular component biogenesis), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0033554 (cellular response to stress), GO:0044248 (cellular catabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0016043 (cellular component organization), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0006914 (autophagy), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0016236 (macroautophagy), GO:0010562 (positive regulation of phosphorus metabolic process), GO:0051246 (regulation of protein metabolic process), GO:0051247 (positive regulation of protein metabolic process), GO:0006996 (organelle organization), GO:0051174 (regulation of phosphorus metabolic process), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0007033 (vacuole organization), GO:0031399 (regulation of protein modification process), GO:0006906 (vesicle fusion), GO:1905037 (autophagosome organization), GO:0045937 (positive regulation of phosphate metabolic process), GO:0048284 (organelle fusion), GO:0031401 (positive regulation of protein modification process), GO:0019220 (regulation of phosphate metabolic process), GO:0042325 (regulation of phosphorylation), GO:0001932 (regulation of protein phosphorylation), GO:0000045 (autophagosome assembly), GO:0042327 (positive regulation of phosphorylation), GO:0001934 (positive regulation of protein phosphorylation), GO:0080171 (lytic vacuole organization), GO:0007040 (lysosome organization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0098796 (membrane protein complex), GO:1902494 (catalytic complex), GO:0019898 (extrinsic component of membrane), GO:0005737 (cytoplasm), GO:1990234 (transferase complex), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005942 (phosphatidylinositol 3-kinase complex), GO:0043228 (non-membrane-bounded organelle), GO:0005773 (vacuole), GO:0005942 (phosphatidylinositol 3-kinase complex), GO:0043227 (membrane-bounded organelle), GO:0000323 (lytic vacuole), GO:0030139 (endocytic vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0061695 (transferase complex, transferring phosphorus-containing groups), GO:0035032 (phosphatidylinositol 3-kinase complex, class III), GO:0031982 (vesicle), GO:0035032 (phosphatidylinositol 3-kinase complex, class III), GO:0005764 (lysosome), GO:0045335 (phagocytic vesicle), GO:0097708 (intracellular vesicle)