Protein: Acyl-coenzyme A diphosphatase FITM2 (EC 3.6.1.-) (FIT family protein fitm-2) (Fat storage-inducing transmembrane homolog 2) (Fat storage-inducing transmembrane protein 2) (Fat-inducing protein 2) (Q5CZ37)
Organism: Caenorhabditis elegans
Function: Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate (By similarity). Preferentially hydrolyzes unsaturated long-chain acyl-CoA substrates in the endoplasmic reticulum (ER) lumen (By similarity). This catalytic activity is required for maintaining ER structure and for lipid droplets (LDs) biogenesis, which are lipid storage organelles involved in maintaining lipid and energy homeostasis (By similarity). May directly bind to diacylglycerol (DAGs) and triacylglycerol, which is also important for LD biogenesis (By similarity). May support directional budding of nacent LDs from the ER into the cytosol by reducing DAG levels at sites of LD formation (By similarity). May play a role in the regulation of cell morphology, ER morphology and cytoskeletal organization (By similarity).
Subcellular location: Endoplasmic reticulum membrane {ECO:0000255|HAMAP-Rule:MF_03230}; Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_03230}.
Sequence: MSTRRSSTRADSTTKRPASPNSTPNAALGIFVAIARQILFIDARKVALFYLAFVTVLSFIESRIELDSTYYLVQKHSVLNQYGVKMGWFWTLVIVGPFIWFSSKAHNRRDRDQPIVDVCRLGVGTACWYFSVQFFHKVLALTSMCDKGRTLTRAQCSEKEGVWTPGYDISGHCFLMIYSILIITEEAIAYRHYQQVTDAVHQMDGDREEHDRLTRCIQYFFVAMLFLHAFWFKQIIISVLYYHIFIEEILGAVAAVVCWFVTYRMLYPAGFLASPIRRTVGRK
InterPro domains: - IPR019388: Fat storage-inducing transmembrane protein (family) [5-270]
- IPR046401: Fat storage-inducing transmembrane protein 1/2 (family) [22-281]
Interaction partners: - GB1/RHD3-type G domain-containing protein
- GB1/RHD3-type G domain-containing protein
- Microsomal triglyceride transfer protein homolog
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0016462 (pyrophosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0022414 (reproductive process), GO:0051234 (establishment of localization), GO:0019953 (sexual reproduction), GO:0051641 (cellular localization), GO:0019098 (reproductive behavior), GO:0044237 (cellular metabolic process), GO:0032504 (multicellular organism reproduction), GO:0007610 (behavior), GO:0018991 (oviposition), GO:0044255 (cellular lipid metabolic process), GO:0006629 (lipid metabolic process), GO:0046907 (intracellular transport), GO:0006810 (transport), GO:0007626 (locomotory behavior), GO:0016192 (vesicle-mediated transport), GO:0016197 (endosomal transport), GO:0060179 (male mating behavior), GO:0061024 (membrane organization), GO:0006909 (phagocytosis), GO:0006897 (endocytosis), GO:0010324 (membrane invagination), GO:0099024 (plasma membrane invagination), GO:0006911 (phagocytosis, engulfment)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0016020 (membrane), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0031967 (organelle envelope), GO:0005635 (nuclear envelope), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0031965 (nuclear membrane), GO:0019866 (organelle inner membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0005637 (nuclear inner membrane), GO:0005634 (nucleus)