Protein: NADPH-dependent codeinone reductase 1-3 (Q9SQ68)
Organism: Papaver somniferum (Opium poppy)
Function: NADPH-dependent codeinone reductase involved in biosynthesis of morphinan-type benzylisoquinoline and opiate alkaloids natural products. Reduces codeinone to codeine in the penultimate step in morphine biosynthesis. Can use morphinone, hydrocodone and hydromorphone as substrate during reductive reaction with NADPH as cofactor, and morphine and dihydrocodeine as substrate during oxidative reaction with NADP as cofactor. Converts morphinone to morphine, and neomorphinone to neomorphine. Reduces irreversibly neopinone, a spontaneous isomer of codeinone, to neopine; in planta, neopine levels are limited to low levels
Subcellular location: Cytoplasm, cytosol. Note=Present in the cytosolic part of laticifer cells that secrete latex. Localized to the parietal region of the sieve element cytoplasm
Sequence: MESNGVPMITLSSGIRMPALGMGTAETMVKGTEREKLAFLKAIEVGYRHFDTAAAYQSEECLGEAIAEALQLGLIKSRDELFITSKLWCADAHADLVLPALQNSLRNLKLDYLDLYLIHHPVSLKPGKFVNEIPKDHILPMDYKSVWAAMEECQTLGFTRAIGVCNFSCKKLQELMAAAKIPPVVNQVEMSPTLHQKNLREYCKANNIMITAHSVLGAICAPWGSNAVMDSKVLHQIAVARGKSVAQVSMRWVYQQGASLVVKSFNEGRMKENLKIFDWELTAENMEKISEIPQSRTSSADFLLSPTGPFKTEEEFWDEKD
InterPro domains: - IPR023210: NADP-dependent oxidoreductase domain (domain) [20-292]
- IPR018170: Aldo/keto reductase, conserved site (conserved_site) [46-276]
- IPR020471: Aldo-keto reductase (family) [8-298]
- IPR036812: NAD(P)-dependent oxidoreductase domain superfamily (homologous_superfamily) [7-321]
- IPR044497: Aldo-keto reductase family 4A/B (family) [13-294]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016614 (oxidoreductase activity, acting on CH-OH group of donors), GO:0016616 (oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:0044249 (cellular biosynthetic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006790 (sulfur compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901376 (organic heteropentacyclic compound metabolic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0009821 (alkaloid biosynthetic process), GO:0044272 (sulfur compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0009820 (alkaloid metabolic process), GO:1901378 (organic heteropentacyclic compound biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)