Protein: N-acetylglucosamine-6-phosphate deacetylase (GlcNAc 6-P deacetylase) (EC 3.5.1.25) (P0AF18)
Organism: Escherichia coli (strain K12)
Function: Involved in the first step in the biosynthesis of amino-sugar-nucleotides. Catalyzes the hydrolysis of the N-acetyl group of N-acetylglucosamine-6-phosphate (GlcNAc-6-P) to yield glucosamine 6-phosphate and acetate. In vitro, can also hydrolyze substrate analogs such as N-thioacetyl-D-glucosamine-6-phosphate, N-trifluoroacetyl-D-glucosamine-6-phosphate, N-acetyl-D-glucosamine-6-sulfate, N-acetyl-D-galactosamine-6-phosphate, and N-formyl-D-glucosamine-6-phosphate.
Sequence: MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQRSLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITTSDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLCENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLYNAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATAPAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRMATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ
InterPro domains: - IPR032466: Metal-dependent hydrolase (homologous_superfamily) [54-350]
- IPR006680: Amidohydrolase-related (domain) [50-379]
- IPR011059: Metal-dependent hydrolase, composite domain superfamily (homologous_superfamily) [1-376]
- IPR003764: N-acetylglucosamine-6-phosphate deacetylase (family) [1-382]
Interaction partners: - Glucosamine-6-phosphate deaminase
- N-acetylmuramic acid 6-phosphate etherase
- PTS system N-acetylglucosamine-specific EIICBA component
- DNA-binding transcriptional dual regulator NagC
- Putative N-acetylmannosamine-6-phosphate 2-epimerase
- N-acetyl-D-glucosamine kinase
- Ribonucleotide monophosphatase NagD
- Phosphoglucosamine mutase
- Glutamine--fructose-6-phosphate aminotransferase
- Putative deaminase AgaI
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0043167 (ion binding), GO:0005515 (protein binding), GO:0016810 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds), GO:0046983 (protein dimerization activity), GO:0042802 (identical protein binding), GO:0043169 (cation binding), GO:0019213 (deacetylase activity), GO:0042803 (protein homodimerization activity), GO:0016811 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides), GO:0046872 (metal ion binding), GO:0046914 (transition metal ion binding), GO:0005506 (iron ion binding), GO:0008270 (zinc ion binding), GO:0008198 (ferrous iron binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0009112 (nucleobase metabolic process), GO:0072527 (pyrimidine-containing compound metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0046112 (nucleobase biosynthetic process), GO:0006206 (pyrimidine nucleobase metabolic process), GO:0072528 (pyrimidine-containing compound biosynthetic process), GO:0019856 (pyrimidine nucleobase biosynthetic process), GO:0006207 ('de novo' pyrimidine nucleobase biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)