Protein: Diacylglycerol kinase (H0YA20)
Organism: Homo sapiens (Human)
Function: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Within the adrenocorticotropic hormone signaling pathway, produces phosphatidic acid which in turn activates NR5A1 and subsequent steroidogenic gene transcription. Also functions downstream of the nerve growth factor signaling pathway being specifically activated in the nucleus by the growth factor. Through its diacylglycerol activity also regulates synaptic vesicle endocytosis
Subcellular location: Cell membrane. Cytoplasm, cytoskeleton. Cytoplasm, cytosol. Nucleus matrix. Nucleus speckle. Synapse
Sequence: XPAAAPGHSFRKVTLTKPTFCHLCSDFIWGLAGFLCDVCNFMSHEKCLKHVRIPCTSVAPSLVRVPVAHCFGPRGLHKRKFCAVCRKVLEAPALHCEVCELHLHPDCVPFACSDCRQCHQDGHQDHDTHHHHWREGNLPSGARCEVCRKTCGSSDVLAGVRCEWCGVQAHSLCSAALAPECGFGRLRSLVLPPACVRLLPGGFSKTQSFRIVEAAEPGEGGDGADGSAAVGPGRETQATPESGKQTLKIFDGDDAVRRSQFRLVTVSRLAGAEEVLEAALRAHHIPEDPGHLELCRLPPSSQACDAWAGGKAGSAVISEEGRSPGSGEATPEAWVIRALPRAQEVLKIYPGWLKVGVAYVSVRVTPKSTARSVVLEVLPLLGRQAESPESFQLVEVAMGCRHVQRTMLMDEQPLLDRLQDIRQMSVRQVSQTRFYVAESRDVAPHVSLFVGGLPPGLSPEEYSSLLHEAGATKGAVVLDVACFAEAERLYMLLKDMAVRGRLLTALVLPDLLHAKLPPDSCPLLVFVNPKSGGLKGRDLLCSFRKLLNPHQVFDLTNGGPLPGLHLFSQVPCFRVLVCGGDGTVGWVLGALEETRYRLACPEPSVAILPLGTGNDLGRVLRWGAGYSGEDPFSVLLSVDEADAVLMDRWTILLDAHEAGSAENDTADAEPPKIVQMSNYCGIGIDAELSLDFHQAREEEPGKFTSRLHNKGVYVRVGLQKISHSRSLHKQIRLQVERQEVELPSIEGLIFINIPSWGSGADLWGSDSDTRFEKPRMDDGLLEVVGVTGVVHMGQVQGGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWVQAPGHMIISAAGPKVHMLRKAKQKPRRAGTTRDARADAAPAPESDPR
InterPro domains: - IPR020454: Diacylglycerol/phorbol-ester binding (domain) [5-182]
- IPR001206: Diacylglycerol kinase, catalytic domain (domain) [518-655]
- IPR029071: Ubiquitin-like domain superfamily (homologous_superfamily) [344-426]
- IPR000159: Ras-associating domain (domain) [244-441]
- IPR000756: Diacylglycerol kinase, accessory domain (domain) [675-827]
- IPR017438: Inorganic polyphosphate/ATP-NAD kinase, N-terminal (homologous_superfamily) [521-644]
- IPR056392: Diacylglycerol kinase theta, RNA-binding domain (domain) [444-509]
- IPR002219: Protein kinase C-like, phorbol ester/diacylglycerol-binding domain (domain) [7-183]
- IPR037607: Diacylglycerol kinase (family) [66-855]
- IPR016064: NAD kinase/diacylglycerol kinase-like domain superfamily (homologous_superfamily) [522-846]
- IPR046349: C1-like domain superfamily (homologous_superfamily) [6-186]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0030234 (enzyme regulator activity), GO:0140677 (molecular function activator activity), GO:0016740 (transferase activity), GO:0030545 (signaling receptor regulator activity), GO:0005515 (protein binding), GO:0008134 (transcription factor binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0019899 (enzyme binding), GO:0019207 (kinase regulator activity), GO:0008047 (enzyme activator activity), GO:0030546 (signaling receptor activator activity), GO:0030297 (transmembrane receptor protein tyrosine kinase activator activity), GO:0016301 (kinase activity), GO:0140297 (DNA-binding transcription factor binding), GO:0019209 (kinase activator activity), GO:0019900 (kinase binding), GO:0043274 (phospholipase binding), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0019887 (protein kinase regulator activity), GO:0004143 (diacylglycerol kinase activity), GO:0030295 (protein kinase activator activity), GO:0030296 (protein tyrosine kinase activator activity)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0008152 (metabolic process), GO:0048519 (negative regulation of biological process), GO:0042221 (response to chemical), GO:0006807 (nitrogen compound metabolic process), GO:0009892 (negative regulation of metabolic process), GO:0065008 (regulation of biological quality), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0048523 (negative regulation of cellular process), GO:0007165 (signal transduction), GO:0001775 (cell activation), GO:0009058 (biosynthetic process), GO:0019222 (regulation of metabolic process), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0050817 (coagulation), GO:0009893 (positive regulation of metabolic process), GO:0006950 (response to stress), GO:0007154 (cell communication), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0009966 (regulation of signal transduction), GO:0044255 (cellular lipid metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0006793 (phosphorus metabolic process), GO:0050878 (regulation of body fluid levels), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0007186 (G protein-coupled receptor signaling pathway), GO:0051171 (regulation of nitrogen compound metabolic process), GO:1902074 (response to salt), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0030168 (platelet activation), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0035556 (intracellular signal transduction), GO:0009611 (response to wounding), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0032350 (regulation of hormone metabolic process), GO:0019637 (organophosphate metabolic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901700 (response to oxygen-containing compound), GO:0010817 (regulation of hormone levels), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0007596 (blood coagulation), GO:0006629 (lipid metabolic process), GO:1901698 (response to nitrogen compound), GO:0031325 (positive regulation of cellular metabolic process), GO:0010646 (regulation of cell communication), GO:0009889 (regulation of biosynthetic process), GO:0062012 (regulation of small molecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0046683 (response to organophosphorus), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0006644 (phospholipid metabolic process), GO:0010675 (regulation of cellular carbohydrate metabolic process), GO:0008277 (regulation of G protein-coupled receptor signaling pathway), GO:0010468 (regulation of gene expression), GO:0045017 (glycerolipid biosynthetic process), GO:0090181 (regulation of cholesterol metabolic process), GO:0019932 (second-messenger-mediated signaling), GO:0051591 (response to cAMP), GO:0043412 (macromolecule modification), GO:0046486 (glycerolipid metabolic process), GO:0046885 (regulation of hormone biosynthetic process), GO:0090407 (organophosphate biosynthetic process), GO:0010563 (negative regulation of phosphorus metabolic process), GO:0030258 (lipid modification), GO:0051252 (regulation of RNA metabolic process), GO:0008610 (lipid biosynthetic process), GO:0010629 (negative regulation of gene expression), GO:0010565 (regulation of cellular ketone metabolic process), GO:0043255 (regulation of carbohydrate biosynthetic process), GO:0010562 (positive regulation of phosphorus metabolic process), GO:0070528 (protein kinase C signaling), GO:0051248 (negative regulation of protein metabolic process), GO:1902930 (regulation of alcohol biosynthetic process), GO:0010906 (regulation of glucose metabolic process), GO:0010628 (positive regulation of gene expression), GO:0006638 (neutral lipid metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0006109 (regulation of carbohydrate metabolic process), GO:0046890 (regulation of lipid biosynthetic process), GO:0036211 (protein modification process), GO:0042060 (wound healing), GO:0010243 (response to organonitrogen compound), GO:0031326 (regulation of cellular biosynthetic process), GO:0051247 (positive regulation of protein metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0014070 (response to organic cyclic compound), GO:0007599 (hemostasis), GO:0019216 (regulation of lipid metabolic process), GO:0033198 (response to ATP), GO:0051174 (regulation of phosphorus metabolic process), GO:1902531 (regulation of intracellular signal transduction), GO:0046834 (lipid phosphorylation), GO:0032006 (regulation of TOR signaling), GO:0031400 (negative regulation of protein modification process), GO:0045937 (positive regulation of phosphate metabolic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0019935 (cyclic-nucleotide-mediated signaling), GO:0006111 (regulation of gluconeogenesis), GO:0018193 (peptidyl-amino acid modification), GO:0016310 (phosphorylation), GO:0008654 (phospholipid biosynthetic process), GO:0050810 (regulation of steroid biosynthetic process), GO:0031401 (positive regulation of protein modification process), GO:0010566 (regulation of ketone biosynthetic process), GO:0019218 (regulation of steroid metabolic process), GO:0031399 (regulation of protein modification process), GO:0090030 (regulation of steroid hormone biosynthetic process), GO:0046474 (glycerophospholipid biosynthetic process), GO:0006468 (protein phosphorylation), GO:0006355 (regulation of DNA-templated transcription), GO:0006639 (acylglycerol metabolic process), GO:0019220 (regulation of phosphate metabolic process), GO:0006650 (glycerophospholipid metabolic process), GO:0014074 (response to purine-containing compound), GO:0045936 (negative regulation of phosphate metabolic process), GO:0001932 (regulation of protein phosphorylation), GO:0019933 (cAMP-mediated signaling), GO:0042326 (negative regulation of phosphorylation), GO:0046339 (diacylglycerol metabolic process), GO:0042327 (positive regulation of phosphorylation), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0018105 (peptidyl-serine phosphorylation), GO:0001934 (positive regulation of protein phosphorylation), GO:0018209 (peptidyl-serine modification), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0042325 (regulation of phosphorylation), GO:0001933 (negative regulation of protein phosphorylation), GO:1903432 (regulation of TORC1 signaling), GO:0006654 (phosphatidic acid biosynthetic process), GO:0046473 (phosphatidic acid metabolic process), GO:0050730 (regulation of peptidyl-tyrosine phosphorylation), GO:0010799 (regulation of peptidyl-threonine phosphorylation), GO:0050731 (positive regulation of peptidyl-tyrosine phosphorylation), GO:0010801 (negative regulation of peptidyl-threonine phosphorylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0016604 (nuclear body), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0031974 (membrane-enclosed lumen), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0012505 (endomembrane system), GO:0005654 (nucleoplasm), GO:0031090 (organelle membrane), GO:0043233 (organelle lumen), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0016607 (nuclear speck), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0012506 (vesicle membrane), GO:0005856 (cytoskeleton), GO:0031981 (nuclear lumen), GO:0097708 (intracellular vesicle), GO:0005634 (nucleus)