Protein: Palmitoyl-acyl carrier protein thioesterase, chloroplastic (EC 3.1.2.-) (16:0-acyl-carrier protein thioesterase) (16:0-ACP thioesterase) (Acyl-[acyl-carrier-protein] hydrolase) (Acyl-acyl carrier protein thioesterase B1) (AtFATB1) (Q9SJE2)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Plays an essential role in chain termination during de novo fatty acid synthesis. Possesses high thioesterase activity for palmitoyl-ACP versus other acyl-ACPs. Substrate preference is 16:0 > 18:1 > 18:0 > 16:1. Plays an essential role in the supply of saturated fatty acids necessary for plant growth and seed development. Contributes to 16:0 production particularly in flowers. May be involved in the synthesis of long chain fatty acid.
Subcellular location: Plastid, chloroplast {ECO:0000305}.
Sequence: MVATSATSSFFPVPSSSLDPNGKGNKIGSTNLAGLNSAPNSGRMKVKPNAQAPPKINGKKVGLPGSVDIVRTDTETSSHPAPRTFINQLPDWSMLLAAITTIFLAAEKQWMMLDWKPRRSDMLVDPFGIGRIVQDGLVFRQNFSIRSYEIGADRSASIETVMNHLQETALNHVKTAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTWVSQSGKNGMRRDWLVRDCNTGETLTRASSVWVMMNKLTRRLSKIPEEVRGEIEPYFVNSDPVLAEDSRKLTKIDDKTADYVRSGLTPRWSDLDVNQHVNNVKYIGWILESAPVGIMERQKLKSMTLEYRRECGRDSVLQSLTAVTGCDIGNLATAGDVECQHLLRLQDGAEVVRGRTEWSSKTPTTTWGTAP
InterPro domains: - IPR002864: Acyl-ACP thioesterase, N-terminal hotdog domain (domain) [136-271]
- IPR045023: Acyl-[acyl-carrier-protein] hydrolase FATA/B (family) [81-403]
- IPR049427: Acyl-ACP thioesterase-like, C-terminal domain (domain) [297-400]
- IPR029069: HotDog domain superfamily (homologous_superfamily) [133-399]
- IPR021113: Acyl-ACP-thioesterase, N-terminal (domain) [1-125]
Interaction partners: - 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial
- Long chain acyl-CoA synthetase 9, chloroplastic
- Long chain acyl-CoA synthetase 8
- Long chain acyl-CoA synthetase 1
- Long chain acyl-CoA synthetase 6, peroxisomal
- Long chain acyl-CoA synthetase 4
- Long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic
- Probable acyl-activating enzyme 16, chloroplastic
- Long chain acyl-CoA synthetase 2
- Long chain acyl-CoA synthetase 5
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0016790 (thiolester hydrolase activity), GO:0016289 (CoA hydrolase activity), GO:0047617 (acyl-CoA hydrolase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0006631 (fatty acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:0016053 (organic acid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0006633 (fatty acid biosynthetic process), GO:0019752 (carboxylic acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0072330 (monocarboxylic acid biosynthetic process), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0031975 (envelope), GO:0005737 (cytoplasm), GO:0031090 (organelle membrane), GO:0031967 (organelle envelope), GO:0034357 (photosynthetic membrane), GO:0043229 (intracellular organelle), GO:0019867 (outer membrane), GO:0009536 (plastid), GO:0043228 (non-membrane-bounded organelle), GO:0043227 (membrane-bounded organelle), GO:0009507 (chloroplast), GO:0042651 (thylakoid membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0031976 (plastid thylakoid), GO:0098588 (bounding membrane of organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0009526 (plastid envelope), GO:0042170 (plastid membrane), GO:0031968 (organelle outer membrane), GO:0009579 (thylakoid), GO:0055035 (plastid thylakoid membrane), GO:0009535 (chloroplast thylakoid membrane), GO:0009534 (chloroplast thylakoid)