Protein: D-glucuronyl C5-epimerase (EC 5.1.3.17) (Heparan sulfate C5-epimerase) (Hsepi) (Heparin/heparan sulfate:glucuronic acid C5-epimerase) (Heparosan-N-sulfate-glucuronate 5-epimerase) (O18756)
Organism: Bos taurus (Bovine)
Function: Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins.
Subcellular location: Golgi apparatus membrane {ECO:0000250|UniProtKB:Q9EPS3}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:Q9EPS3}.
Sequence: MRCLAARVNYKTLIIICALFTLVTVLLWNKCSSDKAIQVPRHLSSGFRVDALEKKAAASESNNYVNHMAKQSEEAFPQEQQKAPPVVGGFNNNGGGRVLGLKYEEIDCLINDEHTIKGRREGNEVFLPFTWVEKYFDVYGKVVQYDGYDRFEFSHSYSKVYAQRAPYHPDGVFMSFEGYNVEVRDRVKCISGVEGVPLSTQWGPQGYFYPIQIAQYGLSHYSKNLTEKPPHIEVYETAEDRDKNSKPNDWTVPKGCFMASVADKSRFTNVKQFIAPETSEGVSLQLGNTKDFIISFDLKFLTNGSVSVVLETTEKNQLFTVHYVSNTQLIAFKERDIYYGIGPRTSWSTVTRDLVTDLRKGVGLSNTKAVKPTRIMPKKVVRLIAKGKGFLDNITISTTAHMAAFFAASDWLVRNQDEKGGWPIMVTRKLGEGFKSLEPGWYSAMAQGQAISTLVRAYLLTKDHIFLNSALRATAPYKFLSEQHGVKAVFMNKHDWYEEYPTTPSSFVLNGFMYSLIGLYDLKETAGEKLGKEARSLYERGMESLKAMLPLYDTGSGTIYDLRHFMLGIAPNLARWDYHTTHINQLQLLSTIDESPIFKEFVKRWKSYLKGSRAKHN
InterPro domains: - IPR010598: D-glucuronyl C5-epimerase, C-terminal (domain) [416-607]
- IPR039721: D-glucuronyl C5-epimerase (family) [1-617]
Interaction partners: - Heparan-sulfate 6-O-sulfotransferase
- Heparan sulfate 2-O-sulfotransferase 1
- Exostosin-1
- Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
- Sulfotransferase
- Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2
- Heparan-sulfate 6-O-sulfotransferase
- Heparan-sulfate 6-O-sulfotransferase
- [heparan sulfate]-glucosamine N-sulfotransferase
- Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016853 (isomerase activity), GO:0043167 (ion binding), GO:0016854 (racemase and epimerase activity), GO:0043169 (cation binding), GO:0046872 (metal ion binding), GO:0016857 (racemase and epimerase activity, acting on carbohydrates and derivatives), GO:0005509 (calcium ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044238 (primary metabolic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0019538 (protein metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0009100 (glycoprotein metabolic process), GO:0044272 (sulfur compound biosynthetic process), GO:0015012 (heparan sulfate proteoglycan biosynthetic process), GO:0009101 (glycoprotein biosynthetic process), GO:0006029 (proteoglycan metabolic process), GO:0030166 (proteoglycan biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0031090 (organelle membrane), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0000139 (Golgi membrane)