Protein: L-lactate dehydrogenase (cytochrome) (EC 1.1.2.3) (Cytochrome b2) (Flavocytochrome b2) (FCB2) (L-lactate ferricytochrome c oxidoreductase) (L-LCR) (P00175)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Catalyzes the oxidation of (S)-lactate (L-lactate) to pyruvate with subsequent transfer of electrons to cytochrome c. Is involved in the utilization of (S)-lactate as a sole source of carbon for growth. Can also use ferricyanide as an electron acceptor in vitro.
Subcellular location: Mitochondrion intermembrane space {ECO:0000269|PubMed:11502169, ECO:0000269|PubMed:22984289}.
Sequence: MLKYKPLLKISKNCEAAILRASKTRLNTIRAYGSTVPKSKSFEQDSRKRTQSWTALRVGAILAATSSVAYLNWHNGQIDNEPKLDMNKQKISPAEVAKHNKPDDCWVVINGYVYDLTRFLPNHPGGQDVIKFNAGKDVTAIFEPLHAPNVIDKYIAPEKKLGPLQGSMPPELVCPPYAPGETKEDIARKEQLKSLLPPLDNIINLYDFEYLASQTLTKQAWAYYSSGANDEVTHRENHNAYHRIFFKPKILVDVRKVDISTDMLGSHVDVPFYVSATALCKLGNPLEGEKDVARGCGQGVTKVPQMISTLASCSPEEIIEAAPSDKQIQWYQLYVNSDRKITDDLVKNVEKLGVKALFVTVDAPSLGQREKDMKLKFSNTKAGPKAMKKTNVEESQGASRALSKFIDPSLTWKDIEELKKKTKLPIVIKGVQRTEDVIKAAEIGVSGVVLSNHGGRQLDFSRAPIEVLAETMPILEQRNLKDKLEVFVDGGVRRGTDVLKALCLGAKGVGLGRPFLYANSCYGRNGVEKAIEILRDEIEMSMRLLGVTSIAELKPDLLDLSTLKARTVGVPNDVLYNEVYEGPTLTEFEDA
InterPro domains: - IPR000262: FMN-dependent dehydrogenase (domain) [212-558]
- IPR008259: FMN-dependent alpha-hydroxy acid dehydrogenase, active site (active_site) [451-457]
- IPR018506: Cytochrome b5, heme-binding site (binding_site) [119-126]
- IPR037396: FMN hydroxy acid dehydrogenase domain (domain) [197-563]
- IPR036400: Cytochrome b5-like heme/steroid binding domain superfamily (homologous_superfamily) [86-180]
- IPR013785: Aldolase-type TIM barrel (homologous_superfamily) [194-573]
- IPR037458: L-mandelate/L-lactate dehydrogenase, FMN-binding domain (domain) [206-555]
- IPR001199: Cytochrome b5-like heme/steroid binding domain (domain) [88-165]
Interaction partners: - D-lactate dehydrogenase [cytochrome] 1, mitochondrial
- D-2-hydroxyglutarate--pyruvate transhydrogenase DLD2
- D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3
- Pyruvate kinase 1
- Pyruvate kinase 2
- External NADH-ubiquinone oxidoreductase 1, mitochondrial
- External NADH-ubiquinone oxidoreductase 2, mitochondrial
- Glycerol-3-phosphate dehydrogenase, mitochondrial
- Pyruvate carboxylase 1
- Pyruvate carboxylase 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016614 (oxidoreductase activity, acting on CH-OH group of donors), GO:0016899 (oxidoreductase activity, acting on the CH-OH group of donors, oxygen as acceptor)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0006631 (fatty acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:0016053 (organic acid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0006633 (fatty acid biosynthetic process), GO:0019752 (carboxylic acid metabolic process), GO:0001676 (long-chain fatty acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0072330 (monocarboxylic acid biosynthetic process), GO:0042759 (long-chain fatty acid biosynthetic process), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)