Protein: U6 snRNA-associated Sm-like protein LSm8 (P47093)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Component of the nuclear LSM2-LSM8 complex, which is involved in splicing of nuclear mRNAs. LSM2-LSM8 associates with multiple snRNP complexes containing the U6 snRNA (U4/U6 snRNP, spliceosomal U4/U6.U5 snRNP, and free U6 snRNP). It binds directly to the U6 snRNA and plays a role in the biogenesis and stability of the U6 snRNP and U4/U6 snRNP complexes. It probably also is involved in degradation of nuclear pre-mRNA by targeting them for decapping. LSM2-LSM8 probably is involved in processing of pre-tRNAs, pre-rRNAs and U3 snoRNA. LSM2 is required for processing of pre-tRNAs, pre-rRNAs and U3 snoRNA.
Subcellular location: Nucleus {ECO:0000269|PubMed:14562095}. Cytoplasm {ECO:0000269|PubMed:14562095}.
Sequence: MSATLKDYLNKRVVIIKVDGECLIASLNGFDKNTNLFITNVFNRISKEFICKAQLLRGSEIALVGLIDAENDDSLAPIDEKKVPMLKDTKNKIENEHVIWEKVYESKTK
InterPro domains: - IPR034103: Sm-like protein Lsm8 (domain) [2-85]
- IPR047575: Sm domain (domain) [1-70]
- IPR010920: LSM domain superfamily (homologous_superfamily) [2-82]
- IPR044642: U6 snRNA-associated Sm-like protein Lsm1/8 (family) [2-87]
- IPR001163: Sm domain, eukaryotic/archaea-type (domain) [3-66]
Interaction partners: - LSM complex subunit LSM4
- Small nuclear ribonucleoprotein Sm D3
- Pre-mRNA-splicing factor SNU114
- LSM1-LSM7 complex subunit LSM1
- Pre-mRNA-processing factor 31
- LSM complex subunit LSM2
- 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component
- Small nuclear ribonucleoprotein Sm D2
- LSM complex subunit LSM7
- LSM complex subunit LSM6
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0031123 (RNA 3'-end processing), GO:0031124 (mRNA 3'-end processing), GO:0008334 (histone mRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:1990904 (ribonucleoprotein complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140513 (nuclear protein-containing complex), GO:0120114 (Sm-like protein family complex), GO:0030532 (small nuclear ribonucleoprotein complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0097525 (spliceosomal snRNP complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0097526 (spliceosomal tri-snRNP complex), GO:0005682 (U5 snRNP), GO:0005634 (nucleus), GO:0046540 (U4/U6 x U5 tri-snRNP complex)