Protein: GTPase-activating protein GYP3 (Multicopy suppressor of bud emergence 3) (Protein MSB3) (P48566)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Regulates exocytosis by functioning as a GAP for SEC4. Stimulates specifically the GTPase activity of YPT6. Also required for efficient polarization of the actin patches.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:10679030}. Bud {ECO:0000269|PubMed:10679030}. Bud neck {ECO:0000269|PubMed:10679030}. Note=Localizes to the presumptive bud site, the bud tip and the mother-bud neck.
Sequence: MQNDQQRFSLQNRTVLAHPYKRLGGAFTVKSPSVPNFHDKMHSDHSSSDSALVNGSFRANDHRSVEPSCLGQASPSEHDGNLSVIDLYGDEVESQRAEGEDDDDNNGDNGNEDLEEVHSDDLDLVPDDDNRQRVELEGAASATSANSNGINNTHFDRYGFKKQNNYISEAEYDKWWVEYSQYCVRRKHKWQLLLEKSGLPVTDDSPSRFPSKSERLKRYVRKGIPAEWRGNAWWHFARGQEKLNKNKGVYSQLLRKMKQIKKQNPNEKQVQDLDIIERDLNRTFPDNIHFQSSLHNKEGPPIIKSLRRVLVAFSLYNPKIGYCQSMNFLAGLLLLFLDEERAFWMLVIITSRYLPGVHNINLEGVNIDQGVLMLCVKEYIPEVWSYIKPSIDHHQKNNKTFSPSNKKVLFNMQKNEFLYRLPPITLCTASWFMSCFVGVVPIETTLRIWDCLFYEESHFLFKVSLAVLKLSEHDLSKIKPRNNSLNYSWGSNLNQRGGSMGQEDSDMEIFQVIQTFPKTLLNPNEIFEKIIFKRRFNLNRLDQDEIDRCRKFVAAQRLKFKTYGELLGNSTSEADLPINDNTDNKGIHITSDAVNEALSSEVYGFKKSLAGVHWNNSIKEKVKQMRKKKDKGD
InterPro domains: - IPR035969: Rab-GAP-TBC domain superfamily (homologous_superfamily) [207-477]
- IPR000195: Rab-GAP-TBC domain (domain) [220-479]
- IPR050302: Rab GTPase-activating TBC domain-containing protein (family) [120-387]
Interaction partners: - Protein BNI1
- Bud site selection protein 6
- Protein SPA2
- Protein PEA2
- GTPase-activating protein MSB4
- Ras-related protein SEC4
- Cell division control protein 42
- Vacuolar protein sorting-associated protein 21
- GTPase-activating protein GYP1
- GTPase-activating protein GYP7
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0030234 (enzyme regulator activity), GO:0140677 (molecular function activator activity), GO:0008047 (enzyme activator activity), GO:0060589 (nucleoside-triphosphatase regulator activity), GO:0030695 (GTPase regulator activity), GO:0005096 (GTPase activator activity)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0051179 (localization), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0030029 (actin filament-based process), GO:0140352 (export from cell), GO:0016043 (cellular component organization), GO:0032940 (secretion by cell), GO:0044085 (cellular component biogenesis), GO:0030036 (actin cytoskeleton organization), GO:0006810 (transport), GO:0046903 (secretion), GO:0016192 (vesicle-mediated transport), GO:0006887 (exocytosis), GO:0006996 (organelle organization), GO:0097435 (supramolecular fiber organization), GO:0007015 (actin filament organization), GO:0022607 (cellular component assembly), GO:0007010 (cytoskeleton organization), GO:0051017 (actin filament bundle assembly), GO:0061572 (actin filament bundle organization), GO:0030046 (parallel actin filament bundle assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005622 (intracellular anatomical structure), GO:0030427 (site of polarized growth), GO:0005933 (cellular bud), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0140535 (intracellular protein-containing complex), GO:0000131 (incipient cellular bud site), GO:0005938 (cell cortex), GO:0005934 (cellular bud tip)