Protein: Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and protein-tyrosine-phosphatase PTEN2A (EC 3.1.3.48) (EC 3.1.3.67) (Protein PHOSPHATASE AND TENSIN HOMOLOG 2-a) (AtPTEN2) (AtPTEN2a) (Q9LT75)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Binds phosphatidic acid. Protein tyrosine phosphatase that exhibits also lipid phosphatase activity. Hydrolyzed poorly p-nitrophenyl phosphate (p-NPP). Can use PtdIns isomers as substrates. Removes efficiently phosphate from the D3 position of the inositol ring, less from the D4 position and not at all from the D5 position on monophosphorylated PtdIns isomers (PIPs). The presence of a phosphate group in the D5 position on PIP(2) isomers reduces lipid phosphatase activity. Mostly active on PtdIns(3)P and PtdIns(3,4)P(2), to a lower extent, on PtdIns(4)P and PtdIns(3,5)P(2), but barely against PtdIns(3,4,5)P(3) as substrate.
Sequence: MSSESPNLPAAAGTVPDNHPPPPPVVTAAEAGSDDSPKGVASKLSAAGISNWAKNLKVPQPFASTQNDSGVENTEKSAFAKFTSGLGIRLSPKSPQTNDTTTEGTSSATESSFIGTITKGLVDTSKNAVKAVQVKARHAVSQNKRRYQEGGFDLDLTYITENIIAMGFPAGDMSSGFFGYVEGFYRNQMEEVINFLETQHKGKYKVYNLCSERLYDVSLFEGKVASFPFDDHNCPPIHLVTSFCQSAYSWLKEDIENVVVVHCKAGMARTGLMICSLLLYLKFFPTAEECMDFYNQKRCVDGKGLVLPSQIRYVKYFERILTYFNGENQPGRRCMLRGFRLHRCPYWIRPSITISDHNGVLFTTKKHPRTKDLSPEDFWFSAPKKGVMVFALPGEPGLTELAGDFKIQFHDRQGDFYCWLNTTMMENRVILKTSELDGFDKRKLPSPGFMVEVVLADINATIPTNPSSETASKTPEETSAANSSPVDGSASVPGPDKETENPDKDDVFSDNEGDSTGPTKTTSSASSQTPEAKKSADETAVLTKATEKVSISGNKGSSQPVQGVTVSKGEATEKPSGAGVNASSSSESEFKVMAADASVFSFGDEDDFESD
InterPro domains: - IPR029021: Protein-tyrosine phosphatase-like (homologous_superfamily) [130-327]
- IPR035892: C2 domain superfamily (homologous_superfamily) [401-456]
- IPR000387: Tyrosine-specific protein phosphatases domain (domain) [259-298]
- IPR014020: Tensin phosphatase, C2 domain (domain) [329-458]
- IPR029023: Tensin-type phosphatase domain (domain) [145-324]
- IPR051281: Dual-specificity lipid and protein phosphatase (family) [123-569]
- IPR045101: PTEN, phosphatase domain (domain) [155-320]
- IPR016130: Protein-tyrosine phosphatase, active site (active_site) [261-271]
- IPR055183: PTEN2A/B, C2 domain (domain) [331-459]
Interaction partners: - Phosphoinositide phosphatase SAC7
- Phosphoinositide phosphatase SAC6
- Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D
- Phosphoinositide phosphatase SAC8
- Phosphatidylinositol 3-kinase VPS34
- Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C
- Phosphatidylinositol 4-kinase alpha 1
- 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B
- 1-phosphatidylinositol-3-phosphate 5-kinase FAB1A
- CDP-diacylglycerol--inositol 3-phosphatidyltransferase 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0042578 (phosphoric ester hydrolase activity), GO:0016791 (phosphatase activity), GO:0052866 (phosphatidylinositol phosphate phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0009628 (response to abiotic stimulus), GO:0006950 (response to stress), GO:0006970 (response to osmotic stress), GO:0009651 (response to salt stress)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)