Protein: Cytochrome P450 81F4 (EC 1.14.-.-) (Q9SZU1)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Involved in indole glucosinolate biosynthesis. Catalyzes hydroxylation reactions of the glucosinolate indole ring. Converts indol-3-yl-methylglucosinolate (I3M) to 1-hydroxy-indol-3-yl-methylglucosinolate (1OH-I3M) intermediate. This hydroxy intermediates is converted to 1-methoxy-indol-3-yl-methylglucosinolate (1MO-I3M) by indole glucosinolate methyltransferase 1 and 2 (IGMT1 and IGMT2).
Subcellular location: Membrane {ECO:0000255}; Single-pass membrane protein {ECO:0000255}.
Sequence: MFNYVIILPLALFLLAYKFFFTSKKQRYYLPPSPSYSLPILGHHLLIKPPVHRLFHRLSNIHGPIFYLRLGSRRAVVISSSSLARECFTGQNDVIVSNRPRFLTSKYIAYNYTTIATTSYGDHWRNLRRICSLEIVSSKRLANFLHIRKEEIQRMLTRLSRDARVGKEVELESILYDLTFNNIVRMVTGKIYYGDDVSDKEEAELFKKLFTFITTNSGARHPGEYLPFMKIFGGSFEKEVKAAAKVIDEMLQRLLDECKSDKDGNTMVNHLLSLQQDDPEYYTDIIIKGLMLGIMVASSETSALTIEWAMASLLNHPKVLDKVKLEIDEIIGQDRLIEESDIANLPYLQNVVSETLRLHPAAPVLVPRSTAEDIKIGGYDVPRDTMVMVNAWAIHRDPDLWTEPERFNPERFNGGEGEKDDVRMLIAFGSGRRICPGVGLAHKIVTLALGSLIQCFDWKKVNEKEIDMSEGPGMAMRMMVPLRALCKTRPIMNKLPAYTKV
InterPro domains: - IPR036396: Cytochrome P450 superfamily (homologous_superfamily) [17-489]
- IPR017972: Cytochrome P450, conserved site (conserved_site) [428-437]
- IPR001128: Cytochrome P450 (family) [32-473]
- IPR002401: Cytochrome P450, E-class, group I (family) [59-458]
- IPR050651: Plant Cytochrome P450 Monooxygenase (family) [12-489]
Interaction partners: - Indole glucosinolate O-methyltransferase 1
- Indole glucosinolate O-methyltransferase 2
- Probable 2-oxoacid dependent dioxygenase
- Indole glucosinolate O-methyltransferase 5
- Cytosolic sulfotransferase 16
- Flavin-containing monooxygenase FMO GS-OX5
- Cytosolic sulfotransferase 18
- Transcription factor MYB34
- Flavin-containing monooxygenase FMO GS-OX2
- Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0004497 (monooxygenase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006790 (sulfur compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0006082 (organic acid metabolic process), GO:0019757 (glycosinolate metabolic process), GO:0042430 (indole-containing compound metabolic process), GO:0019760 (glucosinolate metabolic process), GO:0016143 (S-glycoside metabolic process), GO:1901657 (glycosyl compound metabolic process), GO:0043436 (oxoacid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)