Protein: NADPH oxidase 4 (EC 1.6.3.1) (Kidney oxidase-1) (KOX-1) (Kidney superoxide-producing NADPH oxidase) (Renal NAD(P)H-oxidase) (Superoxide-generating NADPH oxidase 4) (Q9JHI8)
Organism: Mus musculus (Mouse)
Function: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9NPH5}; Multi-pass membrane protein {ECO:0000255}. Cell membrane {ECO:0000250|UniProtKB:Q9NPH5}; Multi-pass membrane protein {ECO:0000255}. Cell junction, focal adhesion {ECO:0000250|UniProtKB:Q924V1}. Nucleus {ECO:0000250|UniProtKB:Q9NPH5}.
Sequence: MAVSWRSWLANEGVKHLCLLIWLSLNVLLFWKTFLLYNQGPEYYYIHQMLGLGLCLSRASASVLNLNCSLILLPMCRTVLAYLRGSQKVPSRRTRRLLDKSKTLHITCGVTICIFSGVHVAAHLVNALNFSVNYSEDFLELNAARYQNEDPRKLLFTTIPGLTGVCMVVVLFLMVTASTYAIRVSNYDIFWYTHNLFFVFYMLLLLHVSGGLLKYQTNVDTHPPGCISLNQTSSQNMSIPDYVSEHFHGSLPRGFSKLEDRYQKTLVKICLEEPKFQAHFPQTWIWISGPLCLYCAERLYRCIRSNKPVTIISVINHPSDVMELRMIKENFKARPGQYIILHCPSVSALENHPFTLTMCPTETKATFGVHFKVVGDWTERFRDLLLPPSSQDSEILPFIHSRNYPKLYIDGPFGSPFEESLNYEVSLCVAGGIGVTPFASILNTLLDDWKPYKLRRLYFIWVCRDIQSFQWFADLLCVLHNKFWQENRPDFVNIQLYLSQTDGIQKIIGEKYHTLNSRLFIGRPRWKLLFDEIAKCNRGKTVGVFCCGPSSISKTLHSLSNRNNSYGTKFEYNKESFS
InterPro domains: - IPR017938: Riboflavin synthase-like beta-barrel (homologous_superfamily) [308-417]
- IPR017927: FAD-binding domain, ferredoxin reductase-type (domain) [304-419]
- IPR050369: Respiratory burst oxidase/Ferric reductase (family) [10-577]
- IPR013121: Ferric reductase, NAD binding domain (domain) [423-560]
- IPR000778: Cytochrome b245, heavy chain (family) [108-445]
- IPR039261: Ferredoxin-NADP reductase (FNR), nucleotide-binding domain (homologous_superfamily) [406-577]
- IPR013112: FAD-binding 8 (domain) [309-416]
- IPR013130: Ferric reductase transmembrane component-like domain (domain) [71-204]
Interaction partners: - Cytochrome b-245 light chain
- Neutrophil cytosol factor 1
- NADPH oxidase 1
- Neutrophil cytosol factor 2
- NADPH oxidase 2
- NADPH oxidase 3
- Toll-like receptor 4
- Neutrophil cytosol factor 4
- NAD
- Polymerase delta-interacting protein 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016651 (oxidoreductase activity, acting on NAD(P)H), GO:0050664 (oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0072593 (reactive oxygen species metabolic process), GO:0006801 (superoxide metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0098796 (membrane protein complex), GO:0031984 (organelle subcompartment), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0043229 (intracellular organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005789 (endoplasmic reticulum membrane)