Protein: Protein diaphanous (P48608)
Organism: Drosophila melanogaster (Fruit fly)
Function: Required for cytokinesis in both mitosis and meiosis. Has a role in actin cytoskeleton organization and is essential for many, if not all, actin-mediated events involving membrane invagination. May serve as a mediator between signaling molecules and actin organizers at specific phases of the cell cycle. Possible component of the contractile ring or may control its function.
Subcellular location: Cytoplasm, cytoskeleton {ECO:0000269|PubMed:7821209}. Cleavage furrow {ECO:0000269|PubMed:7821209}. Apical cell membrane {ECO:0000269|PubMed:23853710}. Note=Localizes to the site where the metaphase furrow is anticipated to form, to the growing tip of cellularization furrows, and to contractile rings (PubMed:7821209). Recruited to the apical cell membrane of epithelial cells in a Rho1 dependent manner, enhanced by phosphatidylinositol 4,5-bisphosphate (PtdIns[4,5]P(2)) enrichment established by sktl/skittles and Pten (PubMed:23853710). {ECO:0000269|PubMed:23853710, ECO:0000269|PubMed:7821209}.
Sequence: MSRHEKTKSTGGGLLDSLFGRPSKSKGGTISSGTLAHGGRPVSADNYVVPGVEDFEQYIQQLSVAELDAKFLEIIEDMNIPKDKREPLLAKSKEERQKMIMWHLKGKNSLERSANSRFEKPIDYVEYLQNGEHSTHKVYQCVESLRVALTSNPISWIKEFGVAGIGTIEKLLARSKNNASYEKIEFEAIRCLKAIMNNTWGLNVVLNPDQHSVVLLLAQSLDPRKPQTMCEALKLLASFCIVYERNGYEKVLRAITTIAATSFKASERFRPIVDALFASDQQDPKRDLACHSLIFINTLTNTPTDLNFRLHLRCEIMRMGLYDRLDEFTKIVEASNNENLQQHFKIFNEIREDDFEEFVQRFDNVTFNMDDATDCFDVLKNLVTDTTSEPYFLSILQHLLYIRDDFYFRPAYYQLIEECISQIVFHKGYCDPNFENRNFNIDTSLLLDDIVEKAKAKESKRSEEYEKKIEQLESAKQEAEAKAAHLEEKVKLMEANGVAAPSPNKLPKVNIPMPPPPPGGGGAPPPPPPPMPGRAGGGPPPPPPPPMPGRAGGPPPPPPPPGMGGPPPPPMPGMMRPGGGPPPPPMMMGPMVPVLPHGLKPKKKWDVKNPMKRANWKAIVPAKMSDKAFWVKCQEDKLAQDDFLAELAVKFSSKPVKKEQKDAVDKPTTLTKKNVDLRVLDSKTAQNLAIMLGGSLKHLSYEQIKICLLRCDTDILSSNILQQLIQYLPPPEHLKRLQEIKAKGEPLPPIEQFAATIGEIKRLSPRLHNLNFKLTYADMVQDIKPDIVAGTAACEEIRNSKKFSKILELILLLGNYMNSGSKNEAAFGFEISYLTKLSNTKDADNKQTLLHYLADLVEKKFPDALNFYDDLSHVNKASRVNMDAIQKAMRQMNSAVKNLETDLQNNKVPQCDDDKFSEVMGKFAEECRQQVDVLGKMQLQMEKLYKDLSEYYAFDPSKYTMEEFFADIKTFKDAFQAAHNDNVRVREELEKKRRLQEAREQSAREQQERQQRKKAVVDMDAPQTQEGVMDSLLEALQTGSAFGQRNRQARRQRPAGAERRAQLSRSRSRTRVTNGQLMTREMILNEVLGSA
InterPro domains: - IPR016024: Armadillo-type fold (homologous_superfamily) [67-434]
- IPR044933: Diaphanous, GTPase-binding domain superfamily (homologous_superfamily) [54-106]
- IPR014768: Rho GTPase-binding/formin homology 3 (GBD/FH3) domain (domain) [59-431]
- IPR015425: Formin, FH2 domain (domain) [601-1044]
- IPR051412: Formin Homology Diaphanous subfamily (family) [538-1077]
- IPR011989: Armadillo-like helical (homologous_superfamily) [117-359]
- IPR014767: Diaphanous autoregulatory domain (domain) [1022-1054]
- IPR042201: Formin, FH2 domain superfamily (homologous_superfamily) [762-1056]
- IPR010465: DRF autoregulatory (domain) [1027-1041]
- IPR010472: Formin, FH3 domain (domain) [246-442]
- IPR010473: Formin, GTPase-binding domain (domain) [59-241]
Interaction partners: - Ras-like GTP-binding protein Rho1
- Profilin
- Cdc42 homolog
- Anillin
- Rho-associated protein kinase
- Pebble, isoform B
- Rho-related BTB domain containing, isoform B
- Cofilin/actin-depolymerizing factor homolog
- Cdc42-interacting protein 4, isoform B
- LD12055p
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0040011 (locomotion), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0009605 (response to external stimulus), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0042221 (response to chemical), GO:0042330 (taxis), GO:0071840 (cellular component organization or biogenesis), GO:0030029 (actin filament-based process), GO:0032989 (cellular component morphogenesis), GO:0000902 (cell morphogenesis), GO:0044085 (cellular component biogenesis), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0048731 (system development), GO:0016043 (cellular component organization), GO:0006935 (chemotaxis), GO:0030036 (actin cytoskeleton organization), GO:0022008 (neurogenesis), GO:0000904 (cell morphogenesis involved in differentiation), GO:0030030 (cell projection organization), GO:0007399 (nervous system development), GO:0097485 (neuron projection guidance), GO:0032990 (cell part morphogenesis), GO:0043933 (protein-containing complex organization), GO:0006996 (organelle organization), GO:0048858 (cell projection morphogenesis), GO:0030182 (neuron differentiation), GO:0097435 (supramolecular fiber organization), GO:0048666 (neuron development), GO:0007015 (actin filament organization), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0007010 (cytoskeleton organization), GO:0048667 (cell morphogenesis involved in neuron differentiation), GO:0007411 (axon guidance), GO:0031175 (neuron projection development), GO:0051017 (actin filament bundle assembly), GO:0048699 (generation of neurons), GO:0061572 (actin filament bundle organization), GO:0065003 (protein-containing complex assembly), GO:0120039 (plasma membrane bounded cell projection morphogenesis), GO:0120036 (plasma membrane bounded cell projection organization), GO:0008154 (actin polymerization or depolymerization), GO:0051258 (protein polymerization), GO:0048812 (neuron projection morphogenesis), GO:0061564 (axon development), GO:0140694 (non-membrane-bounded organelle assembly), GO:0007409 (axonogenesis), GO:0030041 (actin filament polymerization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0150034 (distal axon), GO:0042995 (cell projection), GO:0005829 (cytosol), GO:0030017 (sarcomere), GO:0030427 (site of polarized growth), GO:0031674 (I band), GO:0030018 (Z disc), GO:0005737 (cytoplasm), GO:0036379 (myofilament), GO:0099080 (supramolecular complex), GO:0099081 (supramolecular polymer), GO:0043292 (contractile fiber), GO:0120025 (plasma membrane bounded cell projection), GO:0043229 (intracellular organelle), GO:0030426 (growth cone), GO:0016528 (sarcoplasm), GO:0005865 (striated muscle thin filament), GO:0043228 (non-membrane-bounded organelle), GO:0043005 (neuron projection), GO:0099512 (supramolecular fiber), GO:0030016 (myofibril), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0044295 (axonal growth cone), GO:0005856 (cytoskeleton), GO:0030424 (axon), GO:0015629 (actin cytoskeleton)