Protein: Methylsterol monooxygenase 2-2 (EC 1.14.18.11) (EC 1.14.18.9) (Sterol 4-alpha-methyl-oxidase 1) (AtSMO1) (Sterol 4-alpha-methyl-oxidase 2-2) (Q8VWZ8)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Non-heme iron oxygenase involved in sterols biosynthesis by catalyzing the removal of the second methyl group at the C-4 position. 24-ethylidenelophenol and 24-ethyllophenol are the preferred substrates. Together with SMO2-1, required during embryogenesis, probably by maintaining sterols and auxin homeostasis.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:27006488}; Multi-pass membrane protein {ECO:0000255}.
Sequence: MASFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLERQGFLSKYKIQTKNNTPAAQGKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKTLKENGDMKQT
InterPro domains: - IPR006694: Fatty acid hydroxylase (domain) [113-247]
- IPR050307: Sterol Desaturase and Related Enzymes (family) [9-255]
Interaction partners: - Ergosterol biosynthetic protein 28
- 24-methylenesterol C-methyltransferase 2
- Sterol 14-demethylase
- Delta
- Probable 3-beta-hydroxysteroid-Delta
- 24-methylenesterol C-methyltransferase 3
- 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3
- 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
- 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
- At2g33630/F4P9.40
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0004497 (monooxygenase activity), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0016716 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, another compound as one donor, and incorporation of one atom of oxygen)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0016125 (sterol metabolic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:0030258 (lipid modification), GO:1901362 (organic cyclic compound biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0008202 (steroid metabolic process), GO:0016126 (sterol biosynthetic process), GO:0034440 (lipid oxidation), GO:0006694 (steroid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031984 (organelle subcompartment), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0031090 (organelle membrane), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0043231 (intracellular membrane-bounded organelle), GO:0005789 (endoplasmic reticulum membrane)