Protein: V-type proton ATPase subunit F (V-ATPase subunit F) (V-ATPase 14 kDa subunit) (Vacuolar proton pump subunit F) (P50408)
Organism: Rattus norvegicus (Rat)
Function: Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.
Subcellular location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000269|PubMed:32165585}; Peripheral membrane protein {ECO:0000305}. Cytoplasmic vesicle, clathrin-coated vesicle membrane {ECO:0000269|PubMed:32165585}; Peripheral membrane protein {ECO:0000305}.
Sequence: MAGRGKLIAVIGDEDTVTGFLLGGIGELNKNRHPNFLVVEKDTTINEIEDTFRQFLNRDDIGIILINQYIAEMVRHALDAHQRSIPAVLEIPSKEHPYDAAKDSILRRAKGMFTAEDLR
InterPro domains: - IPR036906: ATPase, V1 complex, subunit F superfamily (homologous_superfamily) [1-115]
- IPR005772: ATPase, V1 complex, subunit F, eukaryotic (family) [1-119]
- IPR008218: ATPase, V1 complex, subunit F (family) [8-108]
Interaction partners: - V-type proton ATPase subunit H
- V-type proton ATPase subunit D
- V-type proton ATPase subunit E 1
- V-type proton ATPase 21 kDa proteolipid subunit c''
- V-type proton ATPase 16 kDa proteolipid subunit c
- V-type proton ATPase catalytic subunit A
- V-type proton ATPase subunit
- V-type proton ATPase 116 kDa subunit a 1
- V-type proton ATPase subunit B, brain isoform
- V-type proton ATPase proteolipid subunit
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0044877 (protein-containing complex binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0042592 (homeostatic process), GO:0051234 (establishment of localization), GO:0048878 (chemical homeostasis), GO:0019725 (cellular homeostasis), GO:0055085 (transmembrane transport), GO:0006885 (regulation of pH), GO:0050801 (monoatomic ion homeostasis), GO:0055082 (intracellular chemical homeostasis), GO:0030641 (regulation of cellular pH), GO:0034220 (monoatomic ion transmembrane transport), GO:0098660 (inorganic ion transmembrane transport), GO:0006810 (transport), GO:0098655 (monoatomic cation transmembrane transport), GO:0098662 (inorganic cation transmembrane transport), GO:0055080 (monoatomic cation homeostasis), GO:0006873 (intracellular monoatomic ion homeostasis), GO:0051453 (regulation of intracellular pH), GO:0006811 (monoatomic ion transport), GO:0051452 (intracellular pH reduction), GO:0006812 (monoatomic cation transport), GO:1902600 (proton transmembrane transport), GO:0030003 (intracellular monoatomic cation homeostasis), GO:0007035 (vacuolar acidification)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0098793 (presynapse), GO:0098796 (membrane protein complex), GO:0030054 (cell junction), GO:1902494 (catalytic complex), GO:0019898 (extrinsic component of membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0008021 (synaptic vesicle), GO:0016469 (proton-transporting two-sector ATPase complex), GO:0031312 (extrinsic component of organelle membrane), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0045202 (synapse), GO:0030133 (transport vesicle), GO:0005773 (vacuole), GO:1904949 (ATPase complex), GO:0043227 (membrane-bounded organelle), GO:0033178 (proton-transporting two-sector ATPase complex, catalytic domain), GO:0030658 (transport vesicle membrane), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0033180 (proton-transporting V-type ATPase, V1 domain), GO:0012506 (vesicle membrane), GO:0098850 (extrinsic component of synaptic vesicle membrane), GO:0070382 (exocytic vesicle), GO:0030672 (synaptic vesicle membrane), GO:0033176 (proton-transporting V-type ATPase complex), GO:0005774 (vacuolar membrane), GO:0031982 (vesicle), GO:0030659 (cytoplasmic vesicle membrane), GO:0097708 (intracellular vesicle), GO:0099501 (exocytic vesicle membrane), GO:0016471 (vacuolar proton-transporting V-type ATPase complex), GO:0000221 (vacuolar proton-transporting V-type ATPase, V1 domain)