Protein: Mitochondrial intermediate peptidase (MIP) (EC 3.4.24.59) (Octapeptidyl aminopeptidase) (Q10415)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane (By similarity).
Subcellular location: Mitochondrion matrix {ECO:0000250}.
Sequence: MQVRTLLTLGKKKVIGNRQCILSLYRKYSNVQSRKAEDQLLRQIFDDQNIAVNQITKRNGIQGVGLFRNHFLSDKDTGFLRLAETASEKCKAVIEDLLLEDTEDGSIVVSKFDRISNLLCSVIDLFEFVRCAHPDKMVVMKAEEAYSYLFELMNTLNTHQGLYEKLKCSLQQTPTLKDTDPEAYTVGRVFLQDFEKSGVNLESSKRNSFVKKSSESATLGRAFFNNSMNRPQRYLTISKQRLAGSDPYFVRSLSKNDKNFIMIPTVGYEGTQALISVANPDVRKEIYMEGHKGTVEEVELLNSYLRSKAEVAKLVGKSSFADLQLIDKMANAPKHVVEFLENLSLKNSSVLKKILNNLALMKKKELNLNFLPSFDVWDREYYTARYKQSLINQKPSLNPSITNYRRFFSVGTVIQGLSRLFSSLYGLRFVPADISPGEVWHPDVNKVNVYNENDHVMGVIYFDLFARTGKTDGAAHFTIRSSRELDLTSFDDSISLGFDDATNIRVKDNKRYQIPVISLLCNFVRSSGMDPTFLDLWDVKTLFHEMGHAMHSILGHTKYQNLAGTRCATDFVELPSIIMEFFMSNPAVLPLYARYEGTEIPLPVQVLNHHNMVENSSAPLDLQSQICMAMVDQLFHSKVVLDPSFNSIDEVTNVTRKFSGFESAPPAAWYLQFSHLYGYSATYYSYIFDTVLASLIFSKLFAGNPLSREAGEKFRKAILRWGGSRSPWECVAEALEQPILATGGEEAMRRIGSEGIKATSTF
InterPro domains: - IPR024079: Metallopeptidase, catalytic domain superfamily (homologous_superfamily) [409-582]
- IPR045090: Peptidase M3A/M3B (family) [61-735]
- IPR033851: Mitochondrial intermediate peptidase (family) [65-748]
- IPR001567: Peptidase M3A/M3B catalytic domain (domain) [274-749]
- IPR024077: Neurolysin/Thimet oligopeptidase, domain 2 (homologous_superfamily) [205-736]
Interaction partners: - Intermediate cleaving peptidase 55
- Mitochondrial inner membrane protease atp23
- Probable mitochondrial-processing peptidase subunit alpha
- ATPase synthesis protein 25
- UBA domain-containing protein Mud1
- Lon protease homolog, mitochondrial
- Putative zinc metalloproteinase C607.06c
- Probable mitochondrial-processing peptidase subunit beta
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0008233 (peptidase activity), GO:0004175 (endopeptidase activity), GO:0008237 (metallopeptidase activity), GO:0004222 (metalloendopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0042592 (homeostatic process), GO:0009987 (cellular process), GO:0048878 (chemical homeostasis), GO:0051641 (cellular localization), GO:0065008 (regulation of biological quality), GO:0019725 (cellular homeostasis), GO:0006807 (nitrogen compound metabolic process), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0033036 (macromolecule localization), GO:0050801 (monoatomic ion homeostasis), GO:0055082 (intracellular chemical homeostasis), GO:1901564 (organonitrogen compound metabolic process), GO:0046907 (intracellular transport), GO:0006810 (transport), GO:0019538 (protein metabolic process), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0098771 (inorganic ion homeostasis), GO:0031647 (regulation of protein stability), GO:0043170 (macromolecule metabolic process), GO:0045184 (establishment of protein localization), GO:0006839 (mitochondrial transport), GO:0006886 (intracellular protein transport), GO:0071705 (nitrogen compound transport), GO:0055080 (monoatomic cation homeostasis), GO:0006508 (proteolysis), GO:0015031 (protein transport), GO:0072594 (establishment of protein localization to organelle), GO:0051604 (protein maturation), GO:0071702 (organic substance transport), GO:0050821 (protein stabilization), GO:0008104 (protein localization), GO:0006873 (intracellular monoatomic ion homeostasis), GO:0006996 (organelle organization), GO:0010467 (gene expression), GO:0055072 (iron ion homeostasis), GO:0072655 (establishment of protein localization to mitochondrion), GO:0007005 (mitochondrion organization), GO:0033365 (protein localization to organelle), GO:0006605 (protein targeting), GO:0006879 (intracellular iron ion homeostasis), GO:0006626 (protein targeting to mitochondrion), GO:0030003 (intracellular monoatomic cation homeostasis), GO:0016485 (protein processing), GO:0070585 (protein localization to mitochondrion), GO:0034982 (mitochondrial protein processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005739 (mitochondrion), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005759 (mitochondrial matrix), GO:0070013 (intracellular organelle lumen)