Protein: Dihydroorotate dehydrogenase (fumarate) (DHOD) (DHODase) (DHOdehase) (EC 1.3.98.1) (Dihydroorotate oxidase) (P28272)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Catalyzes the conversion of dihydroorotate to orotate with fumarate as the electron acceptor. Molecular oxygen can replace fumarate in vitro. Does not use oxaloacetate or NAD or NADP as electron acceptors.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:1409592}.
Sequence: MTASLTTKFLNNTYENPFMNASGVHCMTTQELDELANSKAGAFITKSATTLEREGNPEPRYISVPLGSINSMGLPNEGIDYYLSYVLNRQKNYPDAPAIFFSVAGMSIDENLNLLRKIQDSEFNGITELNLSCPNVPGKPQVAYDFDLTKETLEKVFAFFKKPLGVKLPPYFDFAHFDIMAKILNEFPLAYVNSINSIGNGLFIDVEKESVVVKPKNGFGGIGGEYVKPTALANVRAFYTRLRPEIKVIGTGGIKSGKDAFEHLLCGASMLQIGTELQKEGVKIFERIEKELKDIMEAKGYTSIDQFRGKLNSI
InterPro domains: - IPR023359: Dihydroorotate dehydrogenase A, chain A, domain 2 (homologous_superfamily) [56-223]
- IPR050074: Dihydroorotate dehydrogenase (family) [5-310]
- IPR012135: Dihydroorotate dehydrogenase, class 1/ 2 (family) [1-313]
- IPR024920: Dihydroorotate dehydrogenase, class 1 (family) [5-311]
- IPR005720: Dihydroorotate dehydrogenase, catalytic (domain) [5-296]
- IPR033886: Dihydroorotate dehydrogenase, class 1A (family) [7-296]
- IPR013785: Aldolase-type TIM barrel (homologous_superfamily) [15-307]
- IPR001295: Dihydroorotate dehydrogenase, conserved site (conserved_site) [41-268]
Interaction partners: - Dihydroorotase
- Orotate phosphoribosyltransferase 1
- Orotidine 5'-phosphate decarboxylase
- Orotate phosphoribosyltransferase 2
- Protein transport protein GOT1
- Pyrimidine pathway regulatory protein 1
- Multifunctional protein URA2
- Probable NADPH:adrenodoxin oxidoreductase, mitochondrial
- Cytochrome c mitochondrial import factor CYC2
- Plasma membrane-associated coenzyme Q6 reductase PGA3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016627 (oxidoreductase activity, acting on the CH-CH group of donors)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0009112 (nucleobase metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0072527 (pyrimidine-containing compound metabolic process), GO:0046112 (nucleobase biosynthetic process), GO:0006206 (pyrimidine nucleobase metabolic process), GO:0072528 (pyrimidine-containing compound biosynthetic process), GO:0019856 (pyrimidine nucleobase biosynthetic process), GO:0006207 ('de novo' pyrimidine nucleobase biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)