Protein: Mycofactocin maturase MftC ([Mycofactocin precursor peptide]-pyrrolidinone derivative synthase) (EC 4.1.99.26) ([Mycofactocin precursor peptide]-tyrosine decarboxylase) (EC 1.3.98.7) (P9WJ79)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Radical S-adenosylmethionine (SAM) enzyme responsible for the first step of the biosynthesis of the enzyme cofactor mycofactocin (MFT). Catalyzes two reactions at the C-terminus of the mycofactocin precursor (the MftA peptide). The first one is the oxidative decarboxylation of the C-terminal L-tyrosine of MftA, forming an unsaturated tyramine moiety. The second reaction is the cross-linking of the tyramine with the penultimate L-valine residue, forming a five-membered lactam ring. Its activity requires the presence of the MftB chaperone.
Sequence: MTSPVPRLIEQFERGLDAPICLTWELTYACNLACVHCLSSSGKRDPGELSTRQCKDIIDELERMQVFYVNIGGGEPTVRPDFWELVDYATAHHVGVKFSTNGVRITPEVATRLAATDYVDVQISLDGATAEVNDAIRGTGSFDMAVRALQNLAAAGFAGVKISVVITRRNVAQLDEFATLASRYGATLRITRLRPSGRGTDVWADLHPTADQQVQLYDWLVSKGERVLTGDSFFHLAPLGQSGALAGLNMCGAGRVVCLIDPVGDVYACPFAIHDHFLAGNVLSDGGFQNVWKNSSLFRELREPQSAGACGSCGHYDSCRGGCMAAKFFTGLPLDGPDPECVQGHSEPALARERHLPRPRADHSRGRRVSKPVPLTLSMRPPKRPCNESPV
InterPro domains: - IPR034391: Cmo-like SPASM domain containing protein (family) [17-323]
- IPR050377: Radical SAM PqqA peptide cyclase/Mycofactocin maturase MftC-like (family) [10-342]
- IPR007197: Radical SAM (domain) [16-232]
- IPR023885: 4Fe4S-binding SPASM domain (domain) [251-331]
- IPR006638: Elp3/MiaA/NifB-like, radical SAM core domain (domain) [20-219]
- IPR017200: Coenzyme PQQ synthesis protein E-like (family) [9-357]
- IPR013785: Aldolase-type TIM barrel (homologous_superfamily) [7-303]
- IPR023913: Mycofactocin maturase MftC (family) [5-352]
Interaction partners: - Peptide chaperone MftB
- Pre-mycofactocin synthase
- Mycofactocin precursor peptide peptidase
- Pre-mycofactocin glycosyltransferase
- Putative mycofactocin biosynthesis transcriptional regulator MftR
- Mycofactocin precursor peptide
- Uncharacterized oxidoreductase Rv2750
- Alcohol dehydrogenase B
- Uncharacterized NAD-dependent oxidoreductase Rv0687
- Probable dehydrogenase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0051540 (metal cluster binding), GO:0016491 (oxidoreductase activity), GO:0016667 (oxidoreductase activity, acting on a sulfur group of donors), GO:0051536 (iron-sulfur cluster binding), GO:0051539 (4 iron, 4 sulfur cluster binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006790 (sulfur compound metabolic process), GO:0006082 (organic acid metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0000096 (sulfur amino acid metabolic process), GO:0044272 (sulfur compound biosynthetic process), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0000097 (sulfur amino acid biosynthetic process), GO:0009069 (serine family amino acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0006534 (cysteine metabolic process), GO:0009070 (serine family amino acid biosynthetic process), GO:0019344 (cysteine biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0005886 (plasma membrane), GO:0009274 (peptidoglycan-based cell wall)