Protein: Pre-mycofactocin synthase (PMFT synthase) (EC 1.4.3.26) (P9WND7)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Involved in the biosynthesis of the enzyme cofactor mycofactocin (MFT). Catalyzes the oxidative deamination of AHDP (3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidin-2-one), forming an alpha-keto amide moiety on the resulting molecule, which is called pre-mycofactocin (PMFT). This reaction occurs via a 5-[(4-hydroxyphenyl)methyl]-3-imino-4,4-dimethylpyrrolidin-2-one intermediate, which converts to PMFT. The alpha-keto amide moiety is the redox-active center for the redox activity of mycofactocin.
Sequence: MAEAWFETVAIAQQRAKRRLPKSVYSSLIAASEKGITVADNVAAFSELGFAPHVIGATDKRDLSTTVMGQEVSLPVIISPTGVQAVDPGGEVAVARAAAARGTVMGLSSFASKPIEEVIAANPKTFFQVYWQGGRDALAERVERARQAGAVGLVVTTDWTFSHGRDWGSPKIPEEMNLKTILRLSPEAITRPRWLWKFAKTLRPPDLRVPNQGRRGEPGPPFFAAYGEWMATPPPTWEDIGWLRELWGGPFMLKGVMRVDDAKRAVDAGVSAISVSNHGGNNLDGTPASIRALPAVSAAVGDQVEVLLDGGIRRGSDVVKAVALGARAVMIGRAYLWGLAANGQAGVENVLDILRGGIDSALMGLGHASVHDLSPADILVPTGFIRDLGVPSRRDV
InterPro domains: - IPR023989: Pre-mycofactocin synthase (family) [3-385]
- IPR000262: FMN-dependent dehydrogenase (domain) [16-376]
- IPR012133: Alpha-hydroxy acid dehydrogenase, FMN-dependent (family) [5-383]
- IPR037396: FMN hydroxy acid dehydrogenase domain (domain) [1-383]
- IPR013785: Aldolase-type TIM barrel (homologous_superfamily) [8-384]
Interaction partners: - Mycofactocin maturase MftC
- Peptide chaperone MftB
- Mycofactocin precursor peptide peptidase
- Pre-mycofactocin glycosyltransferase
- Mycofactocin precursor peptide
- Pyruvate kinase
- Putative L-lactate dehydrogenase
- 2-oxoglutarate oxidoreductase subunit KorA
- Pyruvate carboxylase
- Putative malate oxidoreductase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:0016491 (oxidoreductase activity), GO:0016614 (oxidoreductase activity, acting on CH-OH group of donors), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0032553 (ribonucleotide binding), GO:0016616 (oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor), GO:0010181 (FMN binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:0005996 (monosaccharide metabolic process), GO:0005975 (carbohydrate metabolic process), GO:0044282 (small molecule catabolic process), GO:1901575 (organic substance catabolic process), GO:0006091 (generation of precursor metabolites and energy), GO:0006082 (organic acid metabolic process), GO:0006089 (lactate metabolic process), GO:0019318 (hexose metabolic process), GO:0046365 (monosaccharide catabolic process), GO:0015980 (energy derivation by oxidation of organic compounds), GO:0016052 (carbohydrate catabolic process), GO:0043436 (oxoacid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0045333 (cellular respiration), GO:0019320 (hexose catabolic process), GO:0009060 (aerobic respiration), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0005886 (plasma membrane), GO:0009274 (peptidoglycan-based cell wall)