Protein: Elongator complex protein 4 (AtELP4) (Elongator component 4) (Protein ELONGATA 1) (Q9C778)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (By similarity). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). Promotes organ development by modulating cell division rate. May regulate mechanisms producing carbon assimilates or importing sucrose. Involved in the repression of the abscisic acid (ABA) signaling pathway during seed germination and seedling growth. Required for auxin distribution or signaling. Involved in oxidative stress signaling. Prevents anthocyanin accumulation.
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:Q96EB1}. Nucleus {ECO:0000250|UniProtKB:Q96EB1}.
Sequence: MAAPNVRSSSSFSRNISVVSSPQIPGLKSGPNGTAFISSGIRDLDRILGGGYPLGSLVMVMEDPEAPHHMDLLRTYMSQGLVNNQPLLYASPSKDPKGFLGTLPHPASSKEDKPTAPDPDQGESLRIAWQYRKYLENQKNAIDDYSNDFDMRKPLERQFLSGRPIDCVSLLDSSDLSIAQDHCATFLSKFPRNSSNIASIGRIAIQSFCSPLCEYSEKESDMLSFIRLLKSMLMVSNAVAIVTFPPSLLSPSSSKRLQHMADTLLSIKAIPDGDKELEKLLTGYKDINGFLNIHKVARINTQVPVILEAKTFSMSLKKRRFLALECLNQAPVDGSSGTSYGTSGSCSSKSGALDF
InterPro domains: - IPR008728: Elongator complex protein 4 (family) [1-352]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [19-333]
Interaction partners: - Elongator complex protein 1
- Elongator complex protein 3
- Elongator complex protein 2
- Elongator complex protein 6
- Protein KTI12 homolog
- Elongator complex protein 5
- Radical S-adenosyl methionine domain-containing protein 1, mitochondrial
- Vacuolar-sorting receptor 1
- F25A4.4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0016887 (ATP hydrolysis activity), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:0003677 (DNA binding), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0016462 (pyrophosphatase activity), GO:0003697 (single-stranded DNA binding), GO:0017111 (ribonucleoside triphosphate phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0032502 (developmental process), GO:0040007 (growth), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048589 (developmental growth), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0043170 (macromolecule metabolic process), GO:0043412 (macromolecule modification), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:0010467 (gene expression), GO:0010468 (regulation of gene expression), GO:0016070 (RNA metabolic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0006396 (RNA processing), GO:0009451 (RNA modification), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0034470 (ncRNA processing), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0006400 (tRNA modification), GO:0034660 (ncRNA metabolic process), GO:0002097 (tRNA wobble base modification), GO:0006399 (tRNA metabolic process), GO:0008033 (tRNA processing), GO:0002098 (tRNA wobble uridine modification)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)