Protein: Aconitate hydratase 3, mitochondrial (Aconitase 3) (mACO1) (EC 4.2.1.3) (Citrate hydro-lyase 3) (Q9SIB9)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. Contributes to oxidative stress tolerance. Modulates cytosolic citrate metabolism during lipid mobilization. Required during seedling growth.
Subcellular location: Mitochondrion {ECO:0000269|PubMed:14671022, ECO:0000269|PubMed:25061985}. Cytoplasm {ECO:0000269|PubMed:25061985, ECO:0000269|PubMed:25307043}. Note=Cytosolic localization in 3-day-old seedlings, but mitochondrial in 10-day-old plantlets. {ECO:0000269|PubMed:25061985}.
Sequence: MYLTASSSASSSIIRAASSRSSSLFSFRSVLSPSVSSTSPSSLLARRSFGTISPAFRRWSHSFHSKPSPFRFTSQIRAVSPVLDRLQRTFSSMASEHPFKGIFTTLPKPGGGEFGKFYSLPALNDPRVDKLPYSIRILLESAIRNCDNFQVTKEDVEKIIDWEKTSPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFAFLKWGSTAFQNMLVVPPGSGIVHQVNLEYLGRVVFNTKGLLYPDSVVGTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMSMVLPGVVGFKLAGKMRNGVTATDLVLTVTQMLRKHGVVGKFVEFYGNGMSGLSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVAMIEAYLRANNMFVDYNEPQQDRVYSSYLELNLDDVEPCISGPKRPHDRVTLKEMKADWHSCLDSKVGFKGFAIPKEAQEKVVNFSFDGQPAELKHGSVVIAAITSCTNTSNPSVMLGAGLVAKKACDLGLQVKPWIKTSLAPGSGVVTKYLLKSGLQEYLNEQGFNIVGYGCTTCIGNSGEINESVGAAITENDIVAAAVLSGNRNFEGRVHPLTRANYLASPPLVVAYALAGTVNIDFETEPIGKGKNGKDVFLRDIWPTTEEIAEVVQSSVLPDMFRATYESITKGNPMWNKLSVPENTLYSWDPNSTYIHEPPYFKDMTMDPPGPHNVKDAYCLLNFGDSITTDHISPAGNIQKDSPAAKFLMERGVDRKDFNSYGSRRGNDEIMARGTFANIRIVNKLMNGEVGPKTVHIPSGEKLSVFDAAMRYKSSGEDTIILAGAEYGSGSSRDWAAKGPMLQGVKAVIAKSFERIHRSNLVGMGIIPLCFKSGEDADTLGLTGHERYTIHLPTDISEIRPGQDVTVTTDNGKSFTCTVRFDTEVELAYFNHGGILPYVIRNLSKQ
InterPro domains: - IPR015931: Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3 (homologous_superfamily) [96-688]
- IPR015928: Aconitase/3-isopropylmalate dehydratase, swivel (homologous_superfamily) [753-989]
- IPR018136: Aconitase family, 4Fe-4S cluster binding site (binding_site) [525-604]
- IPR001030: Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain (domain) [157-660]
- IPR000573: Aconitase A/isopropylmalate dehydratase small subunit, swivel domain (domain) [789-916]
- IPR044137: Aconitase A, swivel domain (domain) [766-934]
- IPR036008: Aconitase, iron-sulfur domain (homologous_superfamily) [110-725]
- IPR006249: Aconitase/Iron-responsive element-binding protein 2 (family) [92-987]
Interaction partners: - Isocitrate dehydrogenase, chloroplastic/mitochondrial
- Cytosolic isocitrate dehydrogenase
- Peroxisomal isocitrate dehydrogenase
- Citrate synthase 1, peroxisomal
- Citrate synthase 2, peroxisomal
- Citrate synthase 5, mitochondrial
- Citrate synthase 4, mitochondrial
- Citrate synthase 3, peroxisomal
- Isocitrate lyase
- Isocitrate dehydrogenase catalytic subunit 6, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0043167 (ion binding), GO:0016829 (lyase activity), GO:0016835 (carbon-oxygen lyase activity), GO:0043169 (cation binding), GO:0016836 (hydro-lyase activity), GO:0046872 (metal ion binding), GO:0003994 (aconitate hydratase activity), GO:0046914 (transition metal ion binding), GO:0005507 (copper ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0009605 (response to external stimulus), GO:0006950 (response to stress), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0009991 (response to extracellular stimulus), GO:1901615 (organic hydroxy compound metabolic process), GO:0006066 (alcohol metabolic process), GO:0042594 (response to starvation), GO:0006979 (response to oxidative stress), GO:0006082 (organic acid metabolic process), GO:1902652 (secondary alcohol metabolic process), GO:0043436 (oxoacid metabolic process), GO:0031667 (response to nutrient levels), GO:0019752 (carboxylic acid metabolic process), GO:0006102 (isocitrate metabolic process), GO:0072350 (tricarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0005739 (mitochondrion), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle)