Protein: Peroxisomal ATPase PEX1 (EC 3.6.4.-) (Peroxin-1) (Peroxisomal assembly protein 1) (Peroxisome biogenesis protein PAS1) (P24004)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Component of the PEX1-PEX6 AAA ATPase complex, a protein dislocase complex that mediates the ATP-dependent extraction of the PEX5 receptor from peroxisomal membranes, an essential step for PEX5 recycling. Specifically recognizes PEX5 monoubiquitinated at 'Cys-6', and pulls it out of the peroxisome lumen through the PEX2-PEX10-PEX12 retrotranslocation channel. Extraction by the PEX1-PEX6 AAA ATPase complex is accompanied by unfolding of the TPR repeats and release of bound cargo from PEX5.
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:14562095}. Peroxisome membrane {ECO:0000269|PubMed:14562095}; Peripheral membrane protein {ECO:0000269|PubMed:14562095}; Cytoplasmic side {ECO:0000269|PubMed:14562095}.
Sequence: MTTTKRLKFENLRIQFSNAIVGNFLRLPHSIINVLESTNYAIQEFGIAVHSHNSDIPIVHLGWDGHDSGSSENVVLINPVLATVYDLNQKSPLVDLYIQRYDHTHLATEVYVTPETSDDWEIIDANAMRFQNGEILHQTRIVTPGETLICYLEGIVTKFKIDRVEPSMKSARITDGSLVVVAPKVNKTRLVKAEYGHSNKTILKNGAIQLLKKVILRSTVCKMDFPKDNLFVVYISDGAQLPSQKGYASIVKCSLRQSKKSDSDNKSVGIPSKKIGVFIKCDSQIPENHIALSSHLWDAFFTHPMNGAKIKLEFLQMNQANIISGRNATVNIKYFGKDVPTKSGDQYSKLLGGSLLTNNLILPTEQIIIEIKKGESEQQLCNLNEISNESVQWKVTQMGKEEVKDIIERHLPKHYHVKETGEVSRTSKDEDDFITVNSIKKEMVNYLTSPIIATPAIILDGKQGIGKTRLLKELINEVEKDHHIFVKYADCETLHETSNLDKTQKLIMEWCSFCYWYGPSLIVLDNVEALFGKPQANDGDPSNNGQWDNASKLLNFFINQVTKIFNKDNKRIRVLFSGKQKTQINPLLFDKHFVSETWSLRAPDKHARAKLLEYFFSKNQIMKLNRDLQFSDLSLETEGFSPLDLEIFTEKIFYDLQLERDCDNVVTRELFSKSLSAFTPSALRGVKLTKETNIKWGDIGALANAKDVLLETLEWPTKYEPIFVNCPLRLRSGILLYGYPGCGKTLLASAVAQQCGLNFISVKGPEILNKFIGASEQNIRELFERAQSVKPCILFFDEFDSIAPKRGHDSTGVTDRVVNQLLTQMDGAEGLDGVYILAATSRPDLIDSALLRPGRLDKSVICNIPTESERLDILQAIVNSKDKDTGQKKFALEKNADLKLIAEKTAGFSGADLQGLCYNAYLKSVHRWLSAADQSEVVPGNDNIEYFSINEHGRREENRLRLKTLLQQDVVHETKTSTSAASELTAVVTINDLLEACQETKPSISTSELVKLRGIYDRFQKDRNGEMPNGENSIDIGSRLSLM
InterPro domains: - IPR029067: CDC48 domain 2-like superfamily (homologous_superfamily) [105-183]
- IPR003593: AAA+ ATPase domain (domain) [453-866]
- IPR015342: Peroxisomal ATPase PEX1, N-terminal C-lobe (domain) [108-183]
- IPR041569: AAA ATPase, AAA+ lid domain (domain) [896-936]
- IPR003959: ATPase, AAA-type, core (domain) [458-860]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [399-1012]
- IPR050168: AAA ATPase domain-containing protein (family) [316-1013]
- IPR003960: ATPase, AAA-type, conserved site (conserved_site) [834-852]
Interaction partners: - Peroxisomal ATPase PEX6
- Peroxisomal membrane protein PEX15
- Ubiquitin-conjugating enzyme E2-21 kDa
- Peroxisomal membrane protein PEX13
- Peroxisomal targeting signal receptor
- Peroxisome assembly protein 22
- Peroxisome assembly protein 12
- Peroxisome biogenesis factor 10
- Peroxisomal membrane protein PEX14
- Peroxisomal membrane protein PEX17
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0005215 (transporter activity), GO:0140657 (ATP-dependent activity), GO:0016887 (ATP hydrolysis activity), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0044877 (protein-containing complex binding), GO:0022857 (transmembrane transporter activity), GO:0097159 (organic cyclic compound binding), GO:0140318 (protein transporter activity), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:0140030 (modification-dependent protein binding), GO:0140097 (catalytic activity, acting on DNA), GO:0031593 (polyubiquitin modification-dependent protein binding), GO:0003677 (DNA binding), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0016462 (pyrophosphatase activity), GO:0003690 (double-stranded DNA binding), GO:0043565 (sequence-specific DNA binding), GO:0017111 (ribonucleoside triphosphate phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0044237 (cellular metabolic process), GO:0055085 (transmembrane transport), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0033036 (macromolecule localization), GO:0046907 (intracellular transport), GO:0071806 (protein transmembrane transport), GO:0006810 (transport), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0045184 (establishment of protein localization), GO:0043112 (receptor metabolic process), GO:0006886 (intracellular protein transport), GO:0071705 (nitrogen compound transport), GO:0015031 (protein transport), GO:0072594 (establishment of protein localization to organelle), GO:0071702 (organic substance transport), GO:0065002 (intracellular protein transmembrane transport), GO:0008104 (protein localization), GO:0044743 (protein transmembrane import into intracellular organelle), GO:0043574 (peroxisomal transport), GO:0006996 (organelle organization), GO:0001881 (receptor recycling), GO:0006625 (protein targeting to peroxisome), GO:0033365 (protein localization to organelle), GO:0006605 (protein targeting), GO:0007031 (peroxisome organization), GO:0016558 (protein import into peroxisome matrix), GO:0072663 (establishment of protein localization to peroxisome), GO:0015919 (peroxisomal membrane transport), GO:0072662 (protein localization to peroxisome)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005829 (cytosol), GO:1902494 (catalytic complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0016020 (membrane), GO:0043229 (intracellular organelle), GO:0042579 (microbody), GO:1904949 (ATPase complex), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005777 (peroxisome)