Protein: Alkaline ceramidase YPC1 (EC 3.5.1.-) (Acyl-CoA-independent ceramide synthase) (P38298)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Alkaline ceramidase that hydrolyzes phytoceramide and also dihydroceramide into phytosphingosine or dihydrosphingosine. Prefers phytoceramide. Has also reverse activity as acyl-CoA-independent ceramide synthase, catalyzing synthesis of phytoceramide and dihydroceramide from palmitic acid and phytosphingosine or dihydrosphingosine. Is not responsible for the breakdown of unsaturated ceramide. Preferentially uses very long chain fatty acids (C-24 and C-26) in vivo compared to C-16 in vitro.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:10900202, ECO:0000269|PubMed:24866405}; Multi-pass membrane protein {ECO:0000255}. Note=Localizes to the cortical ER. {ECO:0000269|PubMed:24866405}.
Sequence: MGIFRWNYPESSVPGVWGETTSTIDWCEENYVVSPYIAEWSNTLTNSVFILSAIYTTYSAYKNKLEKRFLLIGFGYGLVGVGSWLFHMTLKYRFQLLDELPMIYAMCIPTWSLVCEAKEALLNGDNHKKVPLFEQIFIGVIIGLAVTTASILYVIYKNVDIHQILFGVQIVVVAATAGSLTYRYVHDPLAKRNLKASMALGAILFLSGYISWLLDIHYCSFWVHVRRSILALPLGVLLEPHGWWHILTGMGIYFYIVSLEHLRVITLNVSCNYQFIWRWKVFPELIWKGRKPSTRYSLELFGPYVEDQSIEVKKEK
InterPro domains: - IPR008901: Alkaline ceramidase (family) [8-288]
Interaction partners: - Ceramide synthase LAG1
- Ceramide synthase LAC1
- Sphingoid long chain base kinase 5
- Sphingoid long chain base kinase 4
- Sphingolipid C4-hydroxylase SUR2
- Inositol phosphosphingolipids phospholipase C
- Serine palmitoyltransferase 1
- Dihydrosphingosine 1-phosphate phosphatase YSR3
- Inositol phosphorylceramide synthase catalytic subunit AUR1
- Serine palmitoyltransferase 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016798 (hydrolase activity, acting on glycosyl bonds), GO:0016810 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds), GO:0004553 (hydrolase activity, hydrolyzing O-glycosyl compounds), GO:0016811 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0044248 (cellular catabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:1901575 (organic substance catabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901565 (organonitrogen compound catabolic process), GO:0006643 (membrane lipid metabolic process), GO:0006672 (ceramide metabolic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0044242 (cellular lipid catabolic process), GO:0046467 (membrane lipid biosynthetic process), GO:0016042 (lipid catabolic process), GO:0008610 (lipid biosynthetic process), GO:0006665 (sphingolipid metabolic process), GO:0030149 (sphingolipid catabolic process), GO:0046513 (ceramide biosynthetic process), GO:0030148 (sphingolipid biosynthetic process), GO:0046514 (ceramide catabolic process), GO:0046466 (membrane lipid catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005773 (vacuole), GO:0031090 (organelle membrane), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000323 (lytic vacuole), GO:0043231 (intracellular membrane-bounded organelle), GO:0005774 (vacuolar membrane), GO:0098588 (bounding membrane of organelle), GO:0000322 (storage vacuole), GO:0000324 (fungal-type vacuole), GO:0098852 (lytic vacuole membrane), GO:0000329 (fungal-type vacuole membrane)