Protein: Thiamine pyrophosphokinase (TPK) (Thiamine kinase) (EC 2.7.6.2) (P35202)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Essential protein, it is the only enzyme in yeast capable of synthesizing thiamine pyrophosphate (TPP).
Sequence: MSEECIENPERIKIGTDLINIRNKMNLKELIHPNEDENSTLLILNQKIDIPRPLFYKIWKLHDLKVCADGAANRLYDYLDDDETLRIKYLPNYIIGDLDSLSEKVYKYYRKNKVTIIKQTTQYSTDFTKCVNLISLHFNSPEFRSLISNKDNLQSNHGIELEKGIHTLYNTMTESLVFSKVTPISLLALGGIGGRFDQTVHSITQLYTLSENASYFKLCYMTPTDLIFLIKKNGTLIEYDPQFRNTCIGNCGLLPIGEATLVKETRGLKWDVKNWPTSVVTGRVSSSNRFVGDNCCFIDTKDDIILNVEIFVDKLIDFL
InterPro domains: - IPR036759: Thiamin pyrophosphokinase, catalytic domain superfamily (homologous_superfamily) [4-223]
- IPR007373: Thiamin pyrophosphokinase, thiamin-binding domain (domain) [234-305]
- IPR016966: Thiamin pyrophosphokinase, eukaryotic (family) [2-318]
- IPR006282: Thiamin pyrophosphokinase (family) [40-306]
- IPR036371: Thiamin pyrophosphokinase, thiamin-binding domain superfamily (homologous_superfamily) [225-315]
- IPR007371: Thiamin pyrophosphokinase, catalytic domain (domain) [56-212]
Interaction partners: - cAMP-dependent protein kinase regulatory subunit
- Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20
- Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21
- Constitutive acid phosphatase
- Repressible acid phosphatase
- Acid phosphatase PHO12
- Acid phosphatase PHO11
- Adenylate kinase
- GTP:AMP phosphotransferase, mitochondrial
- Probable acid phosphatase DIA3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016778 (diphosphotransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006766 (vitamin metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0006793 (phosphorus metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0006767 (water-soluble vitamin metabolic process), GO:0009110 (vitamin biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0052646 (alditol phosphate metabolic process), GO:0090407 (organophosphate biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0042726 (flavin-containing compound metabolic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0042364 (water-soluble vitamin biosynthetic process), GO:0009123 (nucleoside monophosphate metabolic process), GO:0042727 (flavin-containing compound biosynthetic process), GO:0009117 (nucleotide metabolic process), GO:1901293 (nucleoside phosphate biosynthetic process), GO:0009124 (nucleoside monophosphate biosynthetic process), GO:0009165 (nucleotide biosynthetic process), GO:0009260 (ribonucleotide biosynthetic process), GO:0009161 (ribonucleoside monophosphate metabolic process), GO:0009156 (ribonucleoside monophosphate biosynthetic process), GO:0009152 (purine ribonucleotide biosynthetic process), GO:0006188 (IMP biosynthetic process), GO:0009168 (purine ribonucleoside monophosphate biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)