Protein: Glutaredoxin-1 (EC 1.11.1.9) (EC 2.5.1.18) (Glutathione-dependent oxidoreductase 1) (P25373)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Component of the glutathione system which performs several activities such as glutathione-dependent oxidoreductase, glutathione peroxidase and glutathione S-transferase (GST) activity. The disulfide bond functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. In addition, it is also involved in reducing cytosolic protein- and non-protein-disulfides in a coupled system with glutathione reductase. Required for resistance to reactive oxygen species (ROS) by directly reducing hydroperoxides and for the detoxification of ROS-mediated damage. GRX1 is less active as an oxidoreductase than GRX2.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus {ECO:0000269|PubMed:14562095}.
Sequence: MVSQETIKHVKDLIAENEIFVASKTYCPYCHAALNTLFEKLKVPRSKVLVLQLNDMKEGADIQAALYEINGQRTVPNIYINGKHIGGNDDLQELRETGELEELLEPILAN
InterPro domains: - IPR011767: Glutaredoxin active site (active_site) [21-37]
- IPR036249: Thioredoxin-like superfamily (homologous_superfamily) [7-106]
- IPR014025: Glutaredoxin subgroup (domain) [19-95]
- IPR011899: Glutaredoxin, eukaryotic/virial (domain) [19-104]
- IPR002109: Glutaredoxin (domain) [19-85]
Interaction partners: - Glutathione reductase
- Thioredoxin-2
- Thioredoxin-1
- Peroxiredoxin PRX1, mitochondrial
- Monothiol glutaredoxin-3
- Catalase T
- Glutathione peroxidase-like peroxiredoxin HYR1
- Glutathione peroxidase-like peroxiredoxin 1
- Monothiol glutaredoxin-7
- Glutaredoxin-2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016209 (antioxidant activity), GO:0004601 (peroxidase activity), GO:0016740 (transferase activity), GO:0016491 (oxidoreductase activity), GO:0016684 (oxidoreductase activity, acting on peroxide as acceptor), GO:0016667 (oxidoreductase activity, acting on a sulfur group of donors), GO:0004602 (glutathione peroxidase activity), GO:0016765 (transferase activity, transferring alkyl or aryl (other than methyl) groups), GO:0004364 (glutathione transferase activity), GO:0015036 (disulfide oxidoreductase activity), GO:0015038 (glutathione disulfide oxidoreductase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0042221 (response to chemical), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044282 (small molecule catabolic process), GO:0006979 (response to oxidative stress), GO:0046483 (heterocycle metabolic process), GO:0044248 (cellular catabolic process), GO:0019637 (organophosphate metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0006793 (phosphorus metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0006082 (organic acid metabolic process), GO:1901575 (organic substance catabolic process), GO:0006790 (sulfur compound metabolic process), GO:0034599 (cellular response to oxidative stress), GO:0006753 (nucleoside phosphate metabolic process), GO:0044273 (sulfur compound catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0000096 (sulfur amino acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:0062197 (cellular response to chemical stress), GO:0009116 (nucleoside metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0016054 (organic acid catabolic process), GO:0044272 (sulfur compound biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0009063 (amino acid catabolic process), GO:0090407 (organophosphate biosynthetic process), GO:0009262 (deoxyribonucleotide metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0072524 (pyridine-containing compound metabolic process), GO:0009117 (nucleotide metabolic process), GO:0019362 (pyridine nucleotide metabolic process), GO:1901293 (nucleoside phosphate biosynthetic process), GO:0046395 (carboxylic acid catabolic process), GO:0000097 (sulfur amino acid biosynthetic process), GO:0009069 (serine family amino acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0019752 (carboxylic acid metabolic process), GO:0009263 (deoxyribonucleotide biosynthetic process), GO:0006534 (cysteine metabolic process), GO:1901606 (alpha-amino acid catabolic process), GO:0009071 (serine family amino acid catabolic process), GO:0009070 (serine family amino acid biosynthetic process), GO:0019344 (cysteine biosynthetic process), GO:0046128 (purine ribonucleoside metabolic process), GO:0046496 (nicotinamide nucleotide metabolic process), GO:0009165 (nucleotide biosynthetic process), GO:0006164 (purine nucleotide biosynthetic process), GO:0051128 (regulation of cellular component organization), GO:0044260 (cellular macromolecule metabolic process), GO:0042221 (response to chemical), GO:0002709 (regulation of T cell mediated immunity), GO:0019674 (NAD metabolic process), GO:0009435 (NAD biosynthetic process), GO:0019359 (nicotinamide nucleotide biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)