Protein: ISWI chromatin-remodeling complex ATPase ISW1 (EC 3.6.4.-) (P38144)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Catalytic component of ISW1-type complexes, which act by remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. They are involved in coordinating transcriptional repression, activation and elongation phases. The ISW1A complex represses gene expression at initiation through specific positioning of a promoter proximal dinucleosome. The ISW1B complex acts within coding regions to control the amount of RNA polymerase II released into productive elongation and to coordinate elongation with termination and pre-mRNA processing.
Subcellular location: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00624, ECO:0000269|PubMed:14562095}.
Sequence: MAYMLAIANFHFFKFYTRMRKKHENNSCNEKDKDENLFKIILAIFLQEKKKYDCISSGSIMTASEEYLENLKPFQVGLPPHDPESNKKRYLLKDANGKKFDLEGTTKRFEHLLSLSGLFKHFIESKAAKDPKFRQVLDVLEENKANGKGKGKHQDVRRRKTEHEEDAELLKEEDSDDDESIEFQFRESPAYVNGQLRPYQIQGVNWLVSLHKNKIAGILADEMGLGKTLQTISFLGYLRYIEKIPGPFLVIAPKSTLNNWLREINRWTPDVNAFILQGDKEERAELIQKKLLGCDFDVVIASYEIIIREKSPLKKINWEYIIIDEAHRIKNEESMLSQVLREFTSRNRLLITGTPLQNNLHELWALLNFLLPDIFSDAQDFDDWFSSESTEEDQDKIVKQLHTVLQPFLLRRIKSDVETSLLPKKELNLYVGMSSMQKKWYKKILEKDLDAVNGSNGSKESKTRLLNIMMQLRKCCNHPYLFDGAEPGPPYTTDEHLVYNAAKLQVLDKLLKKLKEEGSRVLIFSQMSRLLDILEDYCYFRNYEYCRIDGSTAHEDRIQAIDDYNAPDSKKFVFLLTTRAGGLGINLTSADVVVLYDSDWNPQADLQAMDRAHRIGQKKQVKVFRLVTDNSVEEKILERATQKLRLDQLVIQQNRTSLKKKENKADSKDALLSMIQHGAADVFKSGTSTGSAGTPEPGSGEKGDDIDLDELLLKSENKTKSLNAKYETLGLDDLQKFNQDSAYEWNGQDFKKKIQRDIISPLLLNPTKRERKENYSIDNYYKDVLNTGRSSTPSHPRMPKPHVFHSHQLQPPQLKVLYEKERMWTAKKTGYVPTMDDVKAAYGDISDEEEKKQKLELLKLSVNNSQPLTEEEEKMKADWESEGFTNWNKLEFRKFITVSGKYGRNSIQAIARELAPGKTLEEVRAYAKAFWSNIERIEDYEKYLKIIENEEEKIKRVKMQQEALRRKLSEYKNPFFDLKLKHPPSSNNKRTYSEEEDRFILLMLFKYGLDRDDVYELVRDEIRDCPLFELDFYFRSRTPVELARRGNTLLQCLEKEFNAGIVLDDATKDRMKKEDENGKRIREEFADQTANEKENVDGVESKKAKIEDTSNVGTEQLVAEKIPENETTH
InterPro domains: - IPR000330: SNF2, N-terminal (domain) [199-481]
- IPR049730: SNF2/RAD5-like, C-terminal helicase domain (domain) [516-627]
- IPR044754: Isw1/2, N-terminal (domain) [193-414]
- IPR015194: ISWI, HAND domain (domain) [776-883]
- IPR038718: SNF2-like, N-terminal domain superfamily (homologous_superfamily) [197-413]
- IPR015195: SLIDE domain (domain) [941-1057]
- IPR036306: ISWI, HAND domain superfamily (homologous_superfamily) [767-885]
- IPR001005: SANT/Myb domain (domain) [883-1052]
- IPR009057: Homedomain-like superfamily (homologous_superfamily) [886-1058]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [177-731]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [192-384]
- IPR001650: Helicase, C-terminal domain-like (domain) [503-657]
- IPR017884: SANT domain (domain) [882-935]
Interaction partners: - ISWI one complex protein 3
- ISWI one complex protein 2
- Histone H3
- Histone H4
- ISWI one complex protein 4
- Imitation switch two complex protein 1
- ISWI chromatin-remodeling complex ATPase ISW2
- DNA polymerase epsilon subunit D
- Histone H2A.2
- Protein ESC8
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:1901363 (heterocyclic compound binding), GO:0008094 (ATP-dependent activity, acting on DNA), GO:0003682 (chromatin binding), GO:0097159 (organic cyclic compound binding), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0140097 (catalytic activity, acting on DNA), GO:0003677 (DNA binding), GO:0003690 (double-stranded DNA binding), GO:0043565 (sequence-specific DNA binding), GO:0003697 (single-stranded DNA binding), GO:1990837 (sequence-specific double-stranded DNA binding), GO:0019237 (centromeric DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009058 (biosynthetic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0009892 (negative regulation of metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0048523 (negative regulation of cellular process), GO:0044238 (primary metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0016043 (cellular component organization), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0006325 (chromatin organization), GO:0010468 (regulation of gene expression), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:1990700 (nucleolar chromatin organization), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0006996 (organelle organization), GO:0051253 (negative regulation of RNA metabolic process), GO:0010467 (gene expression), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0010629 (negative regulation of gene expression), GO:0016070 (RNA metabolic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0051276 (chromosome organization), GO:0040029 (epigenetic regulation of gene expression), GO:0006338 (chromatin remodeling), GO:0045814 (negative regulation of gene expression, epigenetic), GO:0006997 (nucleus organization), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0032774 (RNA biosynthetic process), GO:0000183 (rDNA heterochromatin formation), GO:0097659 (nucleic acid-templated transcription), GO:0007000 (nucleolus organization), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0031507 (heterochromatin formation), GO:0140747 (regulation of ncRNA transcription), GO:0045892 (negative regulation of DNA-templated transcription), GO:0070828 (heterochromatin organization), GO:0032200 (telomere organization), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0006351 (DNA-templated transcription), GO:0006353 (DNA-templated transcription termination), GO:0006369 (termination of RNA polymerase II transcription), GO:0140718 (facultative heterochromatin formation), GO:0000122 (negative regulation of transcription by RNA polymerase II), GO:0031509 (subtelomeric heterochromatin formation), GO:0006366 (transcription by RNA polymerase II), GO:0140719 (constitutive heterochromatin formation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000785 (chromatin), GO:1902494 (catalytic complex), GO:0140513 (nuclear protein-containing complex), GO:0099080 (supramolecular complex), GO:0099081 (supramolecular polymer), GO:0070603 (SWI/SNF superfamily-type complex), GO:0043229 (intracellular organelle), GO:1904949 (ATPase complex), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0099512 (supramolecular fiber), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031010 (ISWI-type complex), GO:0005856 (cytoskeleton), GO:0099513 (polymeric cytoskeletal fiber), GO:0005694 (chromosome), GO:0005634 (nucleus), GO:0005874 (microtubule), GO:0015630 (microtubule cytoskeleton)