Protein: Histone H3-like centromeric protein hcp-3 (CENP-A homolog) (CeCENP-A) (Holocentric chromosome-binding protein 3) (P34470)
Organism: Caenorhabditis elegans
Function: Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division (By similarity). Might promote cleavage furrow stability during cytokinesis. Not required for chromosome segregation during meiosis.
Subcellular location: Nucleus {ECO:0000269|PubMed:10524621, ECO:0000269|PubMed:11402065}. Chromosome, centromere {ECO:0000269|PubMed:12080088, ECO:0000269|PubMed:15767665, ECO:0000269|PubMed:17339379, ECO:0000269|PubMed:26904949}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:11402065}. Note=Requires knl-2 and lin-53 for chromatin localization (PubMed:17339379, PubMed:26904949). Co-localizes with hcp-6 at prophase, metaphase, and anaphase chromosomes (PubMed:12080088, PubMed:15767665). {ECO:0000269|PubMed:11402065, ECO:0000269|PubMed:12080088, ECO:0000269|PubMed:15767665, ECO:0000269|PubMed:17339379, ECO:0000269|PubMed:26904949}.
Sequence: MADDTPIIEEIAEQNESVTRIMQRLKHDMQRVTSVPGFNTSAAGVNDLIDILNQYKKELEDDAANDYTEAHIHKIRLVTGKRNQYVLKLKQAEDEYHARKEQARRRASSMDFTVGRNSTNLVDYSHGRHHMPSYRRHDSSDEENYSMDGTNGDGNRAGPSNPDRGNRTGPSSSDRVRMRAGRNRVTKTRRYRPGQKALEEIRKYQKTEDLLIQKAPFARLVREIMQTSTPFGADCRIRSDAISALQEAAEAFLVEMFEGSSLISTHAKRVTLMTTDIQLYRRLCLRHL
InterPro domains: - IPR009072: Histone-fold (homologous_superfamily) [153-286]
- IPR000164: Histone H3/CENP-A (family) [171-288]
- IPR007125: Histone H2A/H2B/H3 (domain) [173-283]
Interaction partners: - Kinetochore null protein 2
- Kinetochore protein Nuf2 homolog
- CENP-C-like protein HCP-4
- Aurora/IPL1-related protein kinase 2
- Mitotic checkpoint serine/threonine-protein kinase bub-1
- Probable histone-binding protein lin-53
- JmjC domain-containing protein
- Nucleosome-remodeling factor subunit NURF301-like
- Kinetochore protein ndc-80
- Securin-like protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0042393 (histone binding), GO:0140030 (modification-dependent protein binding), GO:0035064 (methylated histone binding), GO:0140034 (methylation-dependent protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:0016043 (cellular component organization), GO:0007059 (chromosome segregation), GO:1903047 (mitotic cell cycle process), GO:0000278 (mitotic cell cycle), GO:0098813 (nuclear chromosome segregation), GO:0006996 (organelle organization), GO:0000070 (mitotic sister chromatid segregation), GO:0140014 (mitotic nuclear division), GO:0048285 (organelle fission), GO:0000819 (sister chromatid segregation), GO:0051276 (chromosome organization), GO:0000280 (nuclear division)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0032993 (protein-DNA complex), GO:0044815 (DNA packaging complex), GO:0000785 (chromatin), GO:0000786 (nucleosome), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome), GO:0005634 (nucleus)