Protein: Solute carrier family 12 member 2 (Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2) (BSC2) (Na-K-Cl cotransporter 1) (NKCC1) (A0A0G2KTI4)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Cation-chloride cotransporter which mediates the electroneutral transport of chloride, potassium and/or sodium ions across the membrane. Plays a vital role in the regulation of ionic balance and cell volume. Important for maintenance of endolymph volume in the otic vesicle, probably by regulating ion homeostasis. Also plays a role in normal development of the swim bladder.
Subcellular location: Basolateral cell membrane {ECO:0000269|PubMed:19633174, ECO:0000305|PubMed:31367042}; Multi-pass membrane protein {ECO:0000305|PubMed:31367042}.
Sequence: MSASPPISAGDYLSAPEPDALKPAGPTPSQSRFQVDLVTESAGDGETTVGFDSSPPEYVAEPPPDGLRDSVSGGEEAKGRFRVVNFAASSPDAAPAETAQNGDTVMSEGSLHSSTGGQQHHHYDTHTNTYYLRTFGHNTIDAVPKIDFYRQTAAPLGEKLIRPTLSELHDELDKEPFEDGFANGEELTPAEESAAKDVSESKGVVKFGWIKGVLVRCMLNIWGVMLFIRMTWIVGQAGIAYSCIIVIMATVVTTITGCSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVELLMDSGLLMIDQTNDIRVIGTITVILLLGISVAGMEWEAKAQIFLLVILITAIFNYFIGSFIAVDSKKKFGFFSYDAGILAENFGPDFRGQTFFSVFSIFFPAATGILAGANISGDLADPQMAIPKGTLLAILITGLVYVGVAISAGACIVRDATGIESNFTLISNCTDAACKYGYDFSSCRPTVEGEVSSCKFGLHNDFQVMSVVSGFSPLISAGIFSATLSSALASLVSAPKVFQALCKDNIYPGIAIFGKGYGKNNEPLRGYFLTFGIALAFILIAELNVIAPIISNFFLASYALINFSVFHASLANSPGWRPSFKYYNMWASLAGAILCCVVMFIINWWAALLTNVIVLSLYIYVSYKKPDVNWGSSTQALTYHQALTHSLQLCGVADHIKTFRPQCLVMTGAPNSRPAILHLVHAFTKNVGLMLCGHVRISSRRPNFKELNSDMLRYQRWLLNNNSKAFYTCVVAEDLRQGTQYMLQAAGLGRLRPNTLVIGFKNDWRTGDIKEVETYINLIHDAFDFQYGVVILRLREGLDISHIQGQDDSSGMKDVVVSVDISKDSDGDSSKPSSKATSVQNSPAVQKDEDDDGKAHTQPLLKKDKKSPTVPLNVADQRLLDASQQFQQKQGKGTVDVWWLFDDGGLTLLIPYLIANKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDITVLGDINTKPKSEGLTEFAEMIEPYKLREDDMEQEAAEKLKSEEPWRITDNELELYKAKGNRQIRLNELLKEHSSTANLIVMSMPLARKGAVSSALYMAWLDTLSKDLPPILLVRGNHQSVLTFYS
InterPro domains: - IPR004841: Amino acid permease/ SLC12A domain (domain) [212-717]
- IPR018491: SLC12A transporter, C-terminal (domain) [726-1136]
- IPR002443: Solute carrier family 12 member 1/2 (family) [268-672]
- IPR004842: SLC12A transporter family (family) [138-1136]
- IPR002444: Solute carrier family 12 member 2 (family) [29-1052]
- IPR013612: Amino acid permease, N-terminal (domain) [124-175]
Interaction partners: - Cortexin 3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity), GO:1901702 (salt transmembrane transporter activity), GO:0015075 (monoatomic ion transmembrane transporter activity), GO:0015318 (inorganic molecular entity transmembrane transporter activity), GO:0022804 (active transmembrane transporter activity), GO:0008509 (monoatomic anion transmembrane transporter activity), GO:0015079 (potassium ion transmembrane transporter activity), GO:0022890 (inorganic cation transmembrane transporter activity), GO:0015103 (inorganic anion transmembrane transporter activity), GO:0015108 (chloride transmembrane transporter activity), GO:0022853 (active monoatomic ion transmembrane transporter activity), GO:0008324 (monoatomic cation transmembrane transporter activity), GO:0015081 (sodium ion transmembrane transporter activity), GO:0015291 (secondary active transmembrane transporter activity), GO:0015296 (monoatomic anion:monoatomic cation symporter activity), GO:0015293 (symporter activity), GO:0015379 (potassium:chloride symporter activity), GO:0015294 (solute:monoatomic cation symporter activity), GO:0046873 (metal ion transmembrane transporter activity), GO:0015370 (solute:sodium symporter activity), GO:0015377 (chloride:monoatomic cation symporter activity), GO:0015378 (sodium:chloride symporter activity), GO:0015373 (monoatomic anion:sodium symporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0051179 (localization), GO:0032501 (multicellular organismal process), GO:0051234 (establishment of localization), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0055085 (transmembrane transport), GO:0034220 (monoatomic ion transmembrane transport), GO:0098739 (import across plasma membrane), GO:0098660 (inorganic ion transmembrane transport), GO:0048731 (system development), GO:0006810 (transport), GO:0048513 (animal organ development), GO:0072001 (renal system development), GO:0098655 (monoatomic cation transmembrane transport), GO:0098657 (import into cell), GO:0098662 (inorganic cation transmembrane transport), GO:0099587 (inorganic ion import across plasma membrane), GO:0001822 (kidney development), GO:0006811 (monoatomic ion transport), GO:0098659 (inorganic cation import across plasma membrane), GO:0071805 (potassium ion transmembrane transport), GO:0006812 (monoatomic cation transport), GO:0030001 (metal ion transport), GO:1990573 (potassium ion import across plasma membrane), GO:0006813 (potassium ion transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0045178 (basal part of cell), GO:0045177 (apical part of cell), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0009925 (basal plasma membrane), GO:0098590 (plasma membrane region), GO:0005886 (plasma membrane), GO:0016324 (apical plasma membrane)