Protein: 11-beta-hydroxysteroid dehydrogenase type 2 (F1QLP1)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Catalyzes the conversion of biologically active 11beta- hydroxyglucocorticoids (11beta-hydroxysteroid) such as cortisol, to inactive 11-ketoglucocorticoids (11-oxosteroid) such as cortisone, in the presence of NAD(+). Cortisol is the primary glucocorticoid in teleosts and is released to increase glucose bioavailability in order to meet the increased energy demands in response to stress. Functions as a dehydrogenase (oxidase), thereby decreasing the concentration of active glucocorticoids, regulating the hypothalamus-pituitary-interrenal (HPI) axis function in adult fish. Decreasing the excess glucocorticoids may be of relevance to brain function and neural proliferation. Plays a key role by catalyzing the oxidation of 11beta-hydroxytestosterone (11beta,17beta- dihydroxyandrost-4-ene-3-one) to 11-ketotestosterone (17beta- hydroxyandrost-4-ene-3,11-dione), the major fish androgen, that activates androgen receptor transcriptional activity. Catalyzes the conversion of 11beta- hydroxyandrostenedione (11beta-hydroxyandrost-4-ene-3,17-dione) to 11- ketoandrostenedione (androst-4-ene-3,11,17-trione), which can be further metabolized to 11-ketotestosterone. Exerts a dual role in fish by inactivating glucocorticoids and activating androgens
Subcellular location: Membrane; Multi-pass membrane protein
Sequence: MEDFAVSFWIYIGVMSIFVGGAVKKFLAFNIGAMPSVVVWLGATLLVERLCALCMPAVLARLVLCVCCWLYFTWATPKPSLPVEDKAVFITGCDSGFGNATAKKLDAMGFEVFATVLNLEGEGAKHLRKVCSSRLTLLQVDITQPQQVQQALLDTKAKLGIRDLWGLVNNAGWCVNIGDAELSLMSNYRGCMEVNFFGTVTVTRTFLPLLRQSKGRIVTISSPSGEHPFPCLASYGASKAALNLFINTLRHELDPWGVKVSTILPSAYKTGQSSNAEYWEKQYKSLLQGLSPNLLEEYGEEYLLETKELFQNYAKTANEDLSPVIDTIVEALLSPQPQVRYYAGPGLILMYFICSYLPLSISDRFLQKLFVQKKVMPRALIKQQGLSPNDNNNSIKENMNDSSSNNSNFTKCID
InterPro domains: - IPR036291: NAD(P)-binding domain superfamily (homologous_superfamily) [80-373]
- IPR020904: Short-chain dehydrogenase/reductase, conserved site (conserved_site) [222-250]
- IPR002347: Short-chain dehydrogenase/reductase SDR (family) [86-277]
Interaction partners: - Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
- Hydroxysteroid 11-beta-dehydrogenase 1-like protein
- Hydroxysteroid (11-beta) dehydrogenase 1-like b
- 17-beta-hydroxysteroid dehydrogenase type 3
- Mineralocorticoid receptor
- Aromatase
- Cytochrome P450, family 17, subfamily A, polypeptide 1
- Solute carrier family 3 member 2b
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016614 (oxidoreductase activity, acting on CH-OH group of donors), GO:0016229 (steroid dehydrogenase activity), GO:0016616 (oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor), GO:0033764 (steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0032501 (multicellular organismal process), GO:0006950 (response to stress), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0033555 (multicellular organismal response to stress), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0042180 (cellular ketone metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:0006066 (alcohol metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006629 (lipid metabolic process), GO:0120254 (olefinic compound metabolic process), GO:0034308 (primary alcohol metabolic process), GO:1902644 (tertiary alcohol metabolic process), GO:0008202 (steroid metabolic process), GO:0008211 (glucocorticoid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)