Protein: t-SNARE affecting a late Golgi compartment protein 1 (Syntaxin TLG1) (Q03322)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: SNARE protein (of Qc type) involved in membrane fusion probably in retrograde traffic of cytosolic double-membrane vesicles derived from both, early and possibly late endosomes/PVC (prevacuolar compartment) back to the trans-Golgi network (TGN or late Golgi). It has been reported to function both as a (target membrane) t-SNARE and as a (vesicle) v-SNARE. Upon vesicle tethering to the target membrane, which requires additional proteins, a SNARE-pin is formed. This is a very stable 4 parallel alpha-helical coil bundle consisting of 4 SNARE domains (usually one of each type: Qa, Qb, Qc, and R), of which at least one is anchored in the opposite membrane. The formation of the SNARE-pin is believed to bring the two membranes in close proximity and to provide the energy to drive membrane fusion. Through its interaction with the VFT (or GARP) complex, it may also contribute to vesicle recognition specificity and tethering. Regulation of SNARE-pin formation also seems to depend on the phosphorylation state of the protein, phosphorylation by TPK1 causing inhibition and dephosphorylation by SIT4 activation.
Subcellular location: Golgi apparatus, trans-Golgi network membrane {ECO:0000305}; Single-pass type IV membrane protein {ECO:0000305}. Early endosome membrane {ECO:0000305}; Single-pass type IV membrane protein {ECO:0000305}. Late endosome membrane {ECO:0000305}; Single-pass type IV membrane protein {ECO:0000305}. Note=Probably shuttling between TGN and early/late endosome.
Sequence: MNNSEDPFQQVVKDTKEQLNRINNYITRHNTAGDDDQEEEIQDILKDVEETIVDLDRSIIVMKRDENEDVSGREAQVKNIKQQLDALKLRFDRRIQESTQTTIPLEETVENSTLNTSMAENNDGGMSNPFQEQMLREQDVHLDGIHKTMQNLHIQAQTMGDELENQGQLLDNMDEGMDGVVNKLARGRRQLEWVYEKNKEKYDDCCIGLLIVVLIVLLVLAFIA
InterPro domains: - IPR048036: Tlg1p-like, N-terminal domain (domain) [6-92]
- IPR045242: Syntaxin (family) [35-216]
- IPR000727: Target SNARE coiled-coil homology domain (domain) [127-194]
- IPR006012: Syntaxin/epimorphin, conserved site (conserved_site) [138-177]
- IPR010989: SNARE (homologous_superfamily) [5-88]
Interaction partners: - t-SNARE VTI1
- t-SNARE affecting a late Golgi compartment protein 2
- Vacuolar v-SNARE NYV1
- Synaptobrevin homolog 2
- Synaptobrevin homolog YKT6
- Integral membrane protein SED5
- Protein transport protein SEC22
- Synaptobrevin homolog 1
- Vacuolar protein sorting-associated protein 45
- Vacuolar protein sorting-associated protein 51
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0060090 (molecular adaptor activity), GO:0030674 (protein-macromolecule adaptor activity), GO:0005484 (SNAP receptor activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0044237 (cellular metabolic process), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0061919 (process utilizing autophagic mechanism), GO:0009056 (catabolic process), GO:0033036 (macromolecule localization), GO:0006914 (autophagy), GO:0046907 (intracellular transport), GO:0032258 (cytoplasm to vacuole transport by the Cvt pathway), GO:0044248 (cellular catabolic process), GO:0006810 (transport), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0045184 (establishment of protein localization), GO:0016236 (macroautophagy), GO:0006886 (intracellular protein transport), GO:0071705 (nitrogen compound transport), GO:0015031 (protein transport), GO:0072594 (establishment of protein localization to organelle), GO:0071702 (organic substance transport), GO:0016192 (vesicle-mediated transport), GO:0008104 (protein localization), GO:0007034 (vacuolar transport), GO:0006996 (organelle organization), GO:0048193 (Golgi vesicle transport), GO:0033365 (protein localization to organelle), GO:0072666 (establishment of protein localization to vacuole), GO:0006605 (protein targeting), GO:0006896 (Golgi to vacuole transport), GO:0007033 (vacuole organization), GO:0048308 (organelle inheritance), GO:0006623 (protein targeting to vacuole), GO:0000011 (vacuole inheritance), GO:0006892 (post-Golgi vesicle-mediated transport), GO:0072665 (protein localization to vacuole)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0098796 (membrane protein complex), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0031201 (SNARE complex), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0010008 (endosome membrane), GO:0098588 (bounding membrane of organelle), GO:0031982 (vesicle), GO:0030659 (cytoplasmic vesicle membrane), GO:0012506 (vesicle membrane), GO:0097708 (intracellular vesicle)