Protein: E3 ubiquitin-protein ligase TRIM32 (EC 2.3.2.27) (72 kDa Tat-interacting protein) (RING-type E3 ubiquitin transferase TRIM32) (Tripartite motif-containing protein 32) (Zinc finger protein HT2A) (Q13049)
Organism: Homo sapiens (Human)
Function: Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha (By similarity). Also acts as a regulator of autophagy by mediating formation of unanchored 'Lys-63'-linked polyubiquitin chains that activate ULK1: interaction with AMBRA1 is required for ULK1 activation.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:19349376, ECO:0000269|PubMed:37543647}. Mitochondrion {ECO:0000269|PubMed:22745133, ECO:0000269|PubMed:32918979}. Endoplasmic reticulum {ECO:0000269|PubMed:22745133}. Note=Localized in cytoplasmic bodies, often located around the nucleus. {ECO:0000269|PubMed:19349376}.
Sequence: MAAAAASHLNLDALREVLECPICMESFTEEQLRPKLLHCGHTICRQCLEKLLASSINGVRCPFCSKITRITSLTQLTDNLTVLKIIDTAGLSEAVGLLMCRSCGRRLPRQFCRSCGLVLCEPCREADHQPPGHCTLPVKEAAEERRRDFGEKLTRLRELMGELQRRKAALEGVSKDLQARYKAVLQEYGHEERRVQDELARSRKFFTGSLAEVEKSNSQVVEEQSYLLNIAEVQAVSRCDYFLAKIKQADVALLEETADEEEPELTASLPRELTLQDVELLKVGHVGPLQIGQAVKKPRTVNVEDSWAMEATASAASTSVTFREMDMSPEEVVASPRASPAKQRGPEAASNIQQCLFLKKMGAKGSTPGMFNLPVSLYVTSQGEVLVADRGNYRIQVFTRKGFLKEIRRSPSGIDSFVLSFLGADLPNLTPLSVAMNCQGLIGVTDSYDNSLKVYTLDGHCVACHRSQLSKPWGITALPSGQFVVTDVEGGKLWCFTVDRGSGVVKYSCLCSAVRPKFVTCDAEGTVYFTQGLGLNLENRQNEHHLEGGFSIGSVGPDGQLGRQISHFFSENEDFRCIAGMCVDARGDLIVADSSRKEILHFPKGGGYSVLIREGLTCPVGIALTPKGQLLVLDCWDHCIKIYSYHLRRYSTP
InterPro domains: - IPR017907: Zinc finger, RING-type, conserved site (conserved_site) [39-48]
- IPR011042: Six-bladed beta-propeller, TolB-like (homologous_superfamily) [347-652]
- IPR000315: B-box-type zinc finger (domain) [95-138]
- IPR001258: NHL repeat (repeat) [358-646]
- IPR027370: Zinc finger, RING-type, eukaryotic (domain) [20-62]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [6-94]
- IPR047051: TRIM32, B-box type 1 zinc finger (domain) [98-138]
- IPR001841: Zinc finger, RING-type (domain) [20-65]
Interaction partners: - Ubiquitin-conjugating enzyme E2 N
- Protein argonaute-1
- Stimulator of interferon genes protein
- Ubiquitin-conjugating enzyme E2 D1
- E3 ubiquitin-protein ligase TRIM56
- Ribitol 5-phosphate transferase FKRP
- Abl interactor 2
- Calpain-3
- Dysbindin
- Ubiquitin conjugating enzyme E2 V1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:1901363 (heterocyclic compound binding), GO:0097159 (organic cyclic compound binding), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0031369 (translation initiation factor binding), GO:0019787 (ubiquitin-like protein transferase activity), GO:0042802 (identical protein binding), GO:0008092 (cytoskeletal protein binding), GO:0004842 (ubiquitin-protein transferase activity), GO:0017022 (myosin binding), GO:0003723 (RNA binding), GO:0061659 (ubiquitin-like protein ligase activity), GO:0061630 (ubiquitin protein ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0008152 (metabolic process), GO:0042592 (homeostatic process), GO:0048519 (negative regulation of biological process), GO:0048856 (anatomical structure development), GO:0042221 (response to chemical), GO:0051240 (positive regulation of multicellular organismal process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0051239 (regulation of multicellular organismal process), GO:0048871 (multicellular organismal-level homeostasis), GO:0050793 (regulation of developmental process), GO:0019725 (cellular homeostasis), GO:0040017 (positive regulation of locomotion), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0023051 (regulation of signaling), GO:0048523 (negative regulation of cellular process), GO:0040012 (regulation of locomotion), GO:0009628 (response to abiotic stimulus), GO:0048869 (cellular developmental process), GO:0023056 (positive regulation of signaling), GO:0007275 (multicellular organism development), GO:0048584 (positive regulation of response to stimulus), GO:0019222 (regulation of metabolic process), GO:0071704 (organic substance metabolic process), GO:0009893 (positive regulation of metabolic process), GO:0065009 (regulation of molecular function), GO:0030029 (actin filament-based process), GO:0051094 (positive regulation of developmental process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0060759 (regulation of response to cytokine stimulus), GO:2000145 (regulation of cell motility), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0009314 (response to radiation), GO:0048731 (system development), GO:0009966 (regulation of signal transduction), GO:0060255 (regulation of macromolecule metabolic process), GO:0060760 (positive regulation of response to cytokine stimulus), GO:0001817 (regulation of cytokine production), GO:0009894 (regulation of catabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0045595 (regulation of cell differentiation), GO:0016043 (cellular component organization), GO:0001819 (positive regulation of cytokine production), GO:0051962 (positive regulation of nervous system development), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0009967 (positive regulation of signal transduction), GO:0045597 (positive regulation of cell differentiation), GO:0044093 (positive regulation of molecular function), GO:1901564 (organonitrogen compound metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0008285 (negative regulation of cell population proliferation), GO:0019538 (protein metabolic process), GO:0046716 (muscle cell cellular homeostasis), GO:0030036 (actin cytoskeleton organization), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0060249 (anatomical structure homeostasis), GO:2000147 (positive regulation of cell motility), GO:2000026 (regulation of multicellular organismal development), GO:0042127 (regulation of cell population proliferation), GO:0010646 (regulation of cell communication), GO:0051090 (regulation of DNA-binding transcription factor activity), GO:0009889 (regulation of biosynthetic process), GO:0010647 (positive regulation of cell communication), GO:0031323 (regulation of cellular metabolic process), GO:0001894 (tissue homeostasis), GO:0080090 (regulation of primary metabolic process), GO:0009896 (positive regulation of catabolic process), GO:0045664 (regulation of neuron differentiation), GO:0030030 (cell projection organization), GO:0048145 (regulation of fibroblast proliferation), GO:0001959 (regulation of cytokine-mediated signaling pathway), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0032642 (regulation of chemokine production), GO:0051246 (regulation of protein metabolic process), GO:0050769 (positive regulation of neurogenesis), GO:0010468 (regulation of gene expression), GO:0048666 (neuron development), GO:0043412 (macromolecule modification), GO:0007399 (nervous system development), GO:0048147 (negative regulation of fibroblast proliferation), GO:0051252 (regulation of RNA metabolic process), GO:0051091 (positive regulation of DNA-binding transcription factor activity), GO:0001961 (positive regulation of cytokine-mediated signaling pathway), GO:0009416 (response to light stimulus), GO:0034097 (response to cytokine), GO:0045666 (positive regulation of neuron differentiation), GO:0042176 (regulation of protein catabolic process), GO:0032722 (positive regulation of chemokine production), GO:0060284 (regulation of cell development), GO:0010720 (positive regulation of cell development), GO:0010628 (positive regulation of gene expression), GO:0051960 (regulation of nervous system development), GO:0051147 (regulation of muscle cell differentiation), GO:0051149 (positive regulation of muscle cell differentiation), GO:0036211 (protein modification process), GO:0022008 (neurogenesis), GO:0031326 (regulation of cellular biosynthetic process), GO:0051247 (positive regulation of protein metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0045732 (positive regulation of protein catabolic process), GO:0006996 (organelle organization), GO:0045444 (fat cell differentiation), GO:0030182 (neuron differentiation), GO:2001141 (regulation of RNA biosynthetic process), GO:0051092 (positive regulation of NF-kappaB transcription factor activity), GO:0007010 (cytoskeleton organization), GO:0009411 (response to UV), GO:0050767 (regulation of neurogenesis), GO:0048699 (generation of neurons), GO:0070647 (protein modification by small protein conjugation or removal), GO:0051153 (regulation of striated muscle cell differentiation), GO:0120036 (plasma membrane bounded cell projection organization), GO:0006355 (regulation of DNA-templated transcription), GO:0051155 (positive regulation of striated muscle cell differentiation), GO:0031175 (neuron projection development), GO:0034612 (response to tumor necrosis factor), GO:0010803 (regulation of tumor necrosis factor-mediated signaling pathway), GO:0061564 (axon development), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0032446 (protein modification by small protein conjugation), GO:0016567 (protein ubiquitination), GO:0000209 (protein polyubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0099080 (supramolecular complex), GO:0035770 (ribonucleoprotein granule), GO:0036464 (cytoplasmic ribonucleoprotein granule), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0000932 (P-body), GO:0043232 (intracellular non-membrane-bounded organelle)