Protein: CCR4-NOT transcription complex subunit 8 (EC 3.1.13.4) (CAF1-like protein) (CALIFp) (CAF2) (CCR4-associated factor 8) (Caf1b) (Q9UFF9)
Organism: Homo sapiens (Human)
Function: Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT7. Catalytic component of the CCR4-NOT complex which is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:12771185}. Nucleus {ECO:0000269|PubMed:12771185}.
Sequence: MPAALVENSQVICEVWASNLEEEMRKIREIVLSYSYIAMDTEFPGVVVRPIGEFRSSIDYQYQLLRCNVDLLKIIQLGLTFTNEKGEYPSGINTWQFNFKFNLTEDMYSQDSIDLLANSGLQFQKHEEEGIDTLHFAELLMTSGVVLCDNVKWLSFHSGYDFGYMVKLLTDSRLPEEEHEFFHILNLFFPSIYDVKYLMKSCKNLKGGLQEVADQLDLQRIGRQHQAGSDSLLTGMAFFRMKELFFEDSIDDAKYCGRLYGLGTGVAQKQNEDVDSAQEKMSILAIINNMQQ
InterPro domains: - IPR006941: Ribonuclease CAF1 (family) [15-238]
- IPR012337: Ribonuclease H-like superfamily (homologous_superfamily) [12-262]
- IPR039637: CCR4-NOT transcription complex subunit 7/8/Pop2 (family) [11-263]
- IPR036397: Ribonuclease H superfamily (homologous_superfamily) [11-262]
Interaction partners: - CCR4-NOT transcription complex subunit 9
- CCR4-NOT transcription complex subunit 6-like
- CCR4-NOT transcription complex subunit 1
- CCR4-NOT transcription complex subunit 3
- CCR4-NOT transcription complex subunit 6
- CCR4-NOT transcription complex subunit 2
- CCR4-NOT transcription complex subunit 4
- Protein PAT1 homolog 1
- CCR4-NOT transcription complex subunit 10
- CCR4-NOT transcription complex subunit 11
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140110 (transcription regulator activity), GO:0016787 (hydrolase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0003712 (transcription coregulator activity), GO:0005515 (protein binding), GO:0008134 (transcription factor binding), GO:0140098 (catalytic activity, acting on RNA), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0003714 (transcription corepressor activity), GO:0140297 (DNA-binding transcription factor binding), GO:0004518 (nuclease activity), GO:0004540 (ribonuclease activity), GO:0004532 (exoribonuclease activity), GO:0004527 (exonuclease activity), GO:0008408 (3'-5' exonuclease activity), GO:0016896 (RNA exonuclease activity, producing 5'-phosphomonoesters), GO:0016796 (exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters), GO:0000175 (3'-5'-RNA exonuclease activity), GO:0004535 (poly(A)-specific ribonuclease activity)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0008152 (metabolic process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0023057 (negative regulation of signaling), GO:0006807 (nitrogen compound metabolic process), GO:0009892 (negative regulation of metabolic process), GO:0065008 (regulation of biological quality), GO:0002683 (negative regulation of immune system process), GO:0009607 (response to biotic stimulus), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0023051 (regulation of signaling), GO:0048523 (negative regulation of cellular process), GO:0051707 (response to other organism), GO:0048524 (positive regulation of viral process), GO:0019222 (regulation of metabolic process), GO:0002682 (regulation of immune system process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044237 (cellular metabolic process), GO:0009893 (positive regulation of metabolic process), GO:0006950 (response to stress), GO:0050792 (regulation of viral process), GO:0048585 (negative regulation of response to stimulus), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0060759 (regulation of response to cytokine stimulus), GO:0050777 (negative regulation of immune response), GO:0009968 (negative regulation of signal transduction), GO:0045069 (regulation of viral genome replication), GO:0043487 (regulation of RNA stability), GO:0043207 (response to external biotic stimulus), GO:0080134 (regulation of response to stress), GO:0044248 (cellular catabolic process), GO:0009966 (regulation of signal transduction), GO:0060255 (regulation of macromolecule metabolic process), GO:0032101 (regulation of response to external stimulus), GO:0006725 (cellular aromatic compound metabolic process), GO:0060761 (negative regulation of response to cytokine stimulus), GO:0031348 (negative regulation of defense response), GO:0009894 (regulation of catabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0009615 (response to virus), GO:0008284 (positive regulation of cell population proliferation), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0010648 (negative regulation of cell communication), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0045070 (positive regulation of viral genome replication), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0008285 (negative regulation of cell population proliferation), GO:0006952 (defense response), GO:0034641 (cellular nitrogen compound metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0098542 (defense response to other organism), GO:0002831 (regulation of response to biotic stimulus), GO:0050776 (regulation of immune response), GO:0042127 (regulation of cell population proliferation), GO:1903900 (regulation of viral life cycle), GO:0006139 (nucleobase-containing compound metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0010646 (regulation of cell communication), GO:0009889 (regulation of biosynthetic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0002832 (negative regulation of response to biotic stimulus), GO:0009896 (positive regulation of catabolic process), GO:0032102 (negative regulation of response to external stimulus), GO:0031347 (regulation of defense response), GO:0044270 (cellular nitrogen compound catabolic process), GO:0001959 (regulation of cytokine-mediated signaling pathway), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0043488 (regulation of mRNA stability), GO:0019439 (aromatic compound catabolic process), GO:0046700 (heterocycle catabolic process), GO:0045824 (negative regulation of innate immune response), GO:0010468 (regulation of gene expression), GO:0009057 (macromolecule catabolic process), GO:0090304 (nucleic acid metabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0001960 (negative regulation of cytokine-mediated signaling pathway), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0010629 (negative regulation of gene expression), GO:0034249 (negative regulation of amide metabolic process), GO:0034655 (nucleobase-containing compound catabolic process), GO:0045088 (regulation of innate immune response), GO:0051607 (defense response to virus), GO:0051248 (negative regulation of protein metabolic process), GO:0050779 (RNA destabilization), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0034248 (regulation of amide metabolic process), GO:0140546 (defense response to symbiont), GO:0044265 (cellular macromolecule catabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0031331 (positive regulation of cellular catabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031329 (regulation of cellular catabolic process), GO:0051253 (negative regulation of RNA metabolic process), GO:0051174 (regulation of phosphorus metabolic process), GO:0016070 (RNA metabolic process), GO:0061013 (regulation of mRNA catabolic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0031047 (RNA-mediated gene silencing), GO:2000112 (regulation of cellular macromolecule biosynthetic process), GO:1900151 (regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay), GO:0060339 (negative regulation of type I interferon-mediated signaling pathway), GO:1902679 (negative regulation of RNA biosynthetic process), GO:1903311 (regulation of mRNA metabolic process), GO:0017148 (negative regulation of translation), GO:0031399 (regulation of protein modification process), GO:0061014 (positive regulation of mRNA catabolic process), GO:0016441 (post-transcriptional gene silencing), GO:0006402 (mRNA catabolic process), GO:0006355 (regulation of DNA-templated transcription), GO:0006401 (RNA catabolic process), GO:0019220 (regulation of phosphate metabolic process), GO:0006417 (regulation of translation), GO:0060338 (regulation of type I interferon-mediated signaling pathway), GO:0061157 (mRNA destabilization), GO:2000113 (negative regulation of cellular macromolecule biosynthetic process), GO:1903313 (positive regulation of mRNA metabolic process), GO:0010608 (post-transcriptional regulation of gene expression), GO:0001932 (regulation of protein phosphorylation), GO:0045892 (negative regulation of DNA-templated transcription), GO:0016071 (mRNA metabolic process), GO:1900153 (positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0042325 (regulation of phosphorylation), GO:0000956 (nuclear-transcribed mRNA catabolic process), GO:0035194 (RNA-mediated post-transcriptional gene silencing), GO:0060211 (regulation of nuclear-transcribed mRNA poly(A) tail shortening), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:0060213 (positive regulation of nuclear-transcribed mRNA poly(A) tail shortening), GO:0000288 (nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay), GO:0042509 (regulation of tyrosine phosphorylation of STAT protein), GO:0050730 (regulation of peptidyl-tyrosine phosphorylation), GO:0000291 (nuclear-transcribed mRNA catabolic process, exonucleolytic), GO:0035195 (miRNA-mediated gene silencing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0140535 (intracellular protein-containing complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0016604 (nuclear body), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0016607 (nuclear speck), GO:0030014 (CCR4-NOT complex), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)