Protein: E3 ubiquitin-protein ligase makorin-2 (Q9DFG8)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (By similarity). Inhibits neurogenesis and axis formation during embryonic development by modulating the phosphatidylinositol 3-kinase (PI3K) pathway (By similarity). Acts downstream of PI3K and akt1 to up-regulate gsk3b mRNA expression (By similarity)
Subcellular location: Cytoplasm. Nucleus
Sequence: MSTKQVTCRYFLHGVCREGSRCLFSHDLTTSKPSTICKYYQRGACAYGDRCRYDHIKPPGRGSGAPADHSNRSSSSAGASAPGPGPPANTSKHLKKPLVLRDKALCSDSRPRVFSAESSELNECWEQRDDGAQKPHSYLEAIRSGLDASAAAAATAGTFPELQQTSPQICPFLAAGQCQYGESCPYLHGEMCEICRQHVLHPHDPEQRAAHEKKCMVAFEMDMERAFAVQQSQDKVCKICLDVVYEKSSPSERRFGILSSCAHTYCLNCIRQWRCVEQLHNQIRKSCPECRVVSEFVIPSIYWVEDQEQKNLLIEEFKSGVSKKACKYFDQGRGTCPFGGKCFYMHAYADGRRAEPDKPRKQLSAEGNVRFQNSVRLWDFIEEREHRSVPQLEDEVNDLGELFMQLSGASDAPH
InterPro domains: - IPR001841: Zinc finger, RING-type (domain) [237-291]
- IPR018957: Zinc finger, C3HC4 RING-type (domain) [237-290]
- IPR017907: Zinc finger, RING-type, conserved site (conserved_site) [261-270]
- IPR041367: E3 ligase, CCCH-type zinc finger (domain) [36-55]
- IPR036855: Zinc finger, CCCH-type superfamily (homologous_superfamily) [3-189]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [224-313]
- IPR045072: MKRN-like (family) [1-406]
- IPR000571: Zinc finger, CCCH-type (domain) [2-349]
Interaction partners: - Hydroxyacylglutathione hydrolase, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0019787 (ubiquitin-like protein transferase activity), GO:0004842 (ubiquitin-protein transferase activity), GO:0061659 (ubiquitin-like protein ligase activity), GO:0061630 (ubiquitin protein ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0007389 (pattern specification process), GO:0009790 (embryo development), GO:0001756 (somitogenesis), GO:0009888 (tissue development), GO:0003002 (regionalization), GO:0009880 (embryonic pattern specification), GO:0007379 (segment specification), GO:0035282 (segmentation), GO:0061053 (somite development), GO:0060429 (epithelium development), GO:0009952 (anterior/posterior pattern specification), GO:0009792 (embryo development ending in birth or egg hatching), GO:0043009 (chordate embryonic development)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)