Protein: tRNA nucleotidyltransferase cca1 (EC 2.7.7.-) (CC-adding enzyme cca1) (tRNA cytidyltransferase cca1) (Q9UTQ0)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: tRNA nucleotidyltransferase involved in the synthesis of the tRNA CCA terminus. In contrast to what is usually observed in eukaryotes for which one enzyme synthesizes the whole tRNA CCA terminus, in S.pombe, cca1 specifically adds two cytidine residues to a tRNA substrate lacking this sequence while cca2 specifically adds the terminal adenosine residue thereby completing the CCA sequence.
Sequence: MASSSSILELNETEKELSDIFLNVSKKIGQMDRKEPEVRFAGGWVRDKLLRIESHDIDVAIDCMSGFEFAQHLQSYLAQQHPDWETKVIKIDANPLKSKHLETATARIMGMDIDIVNLRHHDYTNSNSSNKLVFGTPLEDALRRDATINALFYNLKSKTVEDFTGKGLVDLSNKIIRTPLVADETFGDDPLRAVRCIRFATKYDFNIHEETIKGLKNPELHERLRSSISRERIGVEVDKMLKHCNTNRALKIIHSLGMFACIFGPLEIHTKKLQSKNIESLSLIPYAIDLFGYLQKKDVSIKNLSSSSKYIFWLAIATLPWYNWSILEKSKIKILPPILIRDSLKYSKPIMSQVENFFVHYPLIMSKINVLEKEGKLTRLGCGRLVRELGPHWRDIIDWAFFMNTLISNSDIQRLNKDEEVTWFHVLVKHIEEYGMEEAYNIQPIINGNEITRILGIRPGPHLRKMLDDSIEWRIQNPESTKEDYIAIMLEKGTSAVVDS
InterPro domains: - IPR043519: Nucleotidyltransferase superfamily (homologous_superfamily) [7-174]
- IPR002646: Poly A polymerase, head domain (domain) [14-177]
Interaction partners: - Glutamyl-tRNA
- tRNA
- Exportin-T
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0005488 (binding), GO:0097367 (carbohydrate derivative binding), GO:0036094 (small molecule binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0016740 (transferase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0043167 (ion binding), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0003676 (nucleic acid binding), GO:0140098 (catalytic activity, acting on RNA), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0000166 (nucleotide binding), GO:0032553 (ribonucleotide binding), GO:0016779 (nucleotidyltransferase activity), GO:0017076 (purine nucleotide binding), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0140101 (catalytic activity, acting on a tRNA), GO:0003723 (RNA binding), GO:0032555 (purine ribonucleotide binding), GO:0005524 (ATP binding), GO:0032559 (adenyl ribonucleotide binding), GO:0030554 (adenyl nucleotide binding), GO:0000049 (tRNA binding), GO:0070566 (adenylyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0034470 (ncRNA processing), GO:0031123 (RNA 3'-end processing), GO:0034660 (ncRNA metabolic process), GO:0042780 (tRNA 3'-end processing), GO:0006399 (tRNA metabolic process), GO:0008033 (tRNA processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)