Protein: High mobility group protein B3 (Q32L31)
Organism: Bos taurus (Bovine)
Function: Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference for non-canonical DNA structures such as single-stranded DNA. Can bend DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters. Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor. Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (By similarity).
Subcellular location: Nucleus {ECO:0000250|UniProtKB:P40618, ECO:0000255|PROSITE-ProRule:PRU00267}. Chromosome {ECO:0000305}. Cytoplasm {ECO:0000250|UniProtKB:O54879}.
Sequence: MAKGDPKKPKGKMSAYAFFVQTCREEHKKKNPEVPVNFAEFSKKCSERWKTMSGKEKSKFDEMAKADKVRYDREMKDYGPAKGGKKKKDPNAPKRPPSGFFLFCSEFRPKIKSANPGISIGDVAKKLGEMWNNLSDSEKQPYINKAAKLKEKYEKDVADYKSKGKFDGAKGAAKVARKKVEEEDEEDEEEEEEEEEEEDE
InterPro domains: - IPR050342: High Mobility Group Box (HMGB) (family) [2-197]
- IPR036910: High mobility group box domain superfamily (homologous_superfamily) [1-171]
- IPR017967: HMG box A DNA-binding domain, conserved site (conserved_site) [41-52]
- IPR009071: High mobility group box domain (domain) [6-162]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0042393 (histone binding), GO:0003677 (DNA binding), GO:0008301 (DNA binding, bending), GO:0003684 (damaged DNA binding), GO:0003690 (double-stranded DNA binding), GO:0003697 (single-stranded DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0032502 (developmental process), GO:0002376 (immune system process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0044237 (cellular metabolic process), GO:0002200 (somatic diversification of immune receptors), GO:0002520 (immune system development), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0048731 (system development), GO:0002562 (somatic diversification of immune receptors via germline recombination within a single locus), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0033151 (V(D)J recombination), GO:0006259 (DNA metabolic process), GO:0006996 (organelle organization), GO:0006265 (DNA topological change), GO:0006310 (DNA recombination), GO:0051276 (chromosome organization), GO:0071103 (DNA conformation change), GO:0016444 (somatic cell DNA recombination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)