Protein: Metal-binding activator 1 (P35192)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Regulatory protein involved in Cu/Fe utilization and stress resistance. Involved in basal level transcription of FRE1 and H(2)O(2)-induced transcription of CTT1. Regulates the transcription of CTR1 and CTR3 via the copper ion responsive elements in their promoters. Required for degradation of CTR1.
Subcellular location: Nucleus.
Sequence: MIIFNGNKYACASCIRGHRSSTCRHSHRMLIKVRTRGRPSPMAIRDAILVDSTSQSTEYENGAQIEGDCCSAMNQQPILFVRASAVRKARMINGKLHILMEEGFTAHEPKDISTFTDDGNKYITETEFLRKHSPKAPATGTISPDSTKSSSSSEKKERSRLQQEPIRHFSNCCKKDKSQNPASNGKTNKAPSDDIFTPYGSLESTSAFNDILQENYNSSVPGAHDSSETLTPQSTTTIAAPHSSDVASKVEVLTHKGIFLSTQCSCEDESCPCVNCLIHRSEEELNSYIQQSGVPLTNIGEAQITDKMMDYLDDCKCTDKECICPPDNCTCDGCFSHSTNIIPFEKFFFYGILNARLTRKTQIKFKGKLVPSKYWWDFLKLQVPLMTDAQLELLDIHAWFQKLVSNYAPHLSDATTS
InterPro domains: - IPR051763: Copper Homeostasis Regulator (family) [1-302]
- IPR036395: Copper fist DNA-binding domain superfamily (homologous_superfamily) [1-41]
- IPR001083: Copper fist DNA-binding domain (domain) [1-40]
Interaction partners: - Copper transport protein CTR3
- Ferric/cupric reductase transmembrane component 1
- Copper transport protein CTR2
- Ferric/cupric reductase transmembrane component 7
- Ferric/cupric reductase transmembrane component 2
- Iron-regulated transcriptional activator AFT1
- Iron transport multicopper oxidase FET3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0140110 (transcription regulator activity), GO:0005488 (binding), GO:0003700 (DNA-binding transcription factor activity), GO:0005515 (protein binding), GO:0001216 (DNA-binding transcription activator activity), GO:0000981 (DNA-binding transcription factor activity, RNA polymerase II-specific), GO:0001228 (DNA-binding transcription activator activity, RNA polymerase II-specific)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0042592 (homeostatic process), GO:0009987 (cellular process), GO:0048878 (chemical homeostasis), GO:0019725 (cellular homeostasis), GO:0048522 (positive regulation of cellular process), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0050801 (monoatomic ion homeostasis), GO:0055082 (intracellular chemical homeostasis), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0098771 (inorganic ion homeostasis), GO:0080090 (regulation of primary metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0055080 (monoatomic cation homeostasis), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0055070 (copper ion homeostasis), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:0006873 (intracellular monoatomic ion homeostasis), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0010468 (regulation of gene expression), GO:0006878 (intracellular copper ion homeostasis), GO:1902680 (positive regulation of RNA biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0030003 (intracellular monoatomic cation homeostasis), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0045944 (positive regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)