Protein: Negative elongation factor E (NELF-E) (RNA-binding protein RD) (P19426)
Organism: Mus musculus (Mouse)
Function: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (By similarity).
Subcellular location: Nucleus {ECO:0000269|PubMed:31399344}. Chromosome {ECO:0000269|PubMed:31399344}. Note=Localizes to chromatin (PubMed:31399344). Phosphorylation by the P-TEFb complex promotes its release from chromatin (PubMed:31399344). {ECO:0000269|PubMed:31399344}.
Sequence: MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSGPASQGGVKRSLSEQPVVDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSMSADEDLQEPSRRPQRKSLYESFVSSSDRLRELGQDGEEAEAPGAGDGPPRGFDWSYEEHGSARSSASPPRSRSRDRSHDRSRDRDRDKERDRDRDRDRDRDRDKDKDRDRDRDRDKERDRDRDRDRDRERDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDL
InterPro domains: - IPR034637: Negative elongation factor E, RNA recognition motif (domain) [262-336]
- IPR035979: RNA-binding domain superfamily (homologous_superfamily) [207-334]
- IPR012677: Nucleotide-binding alpha-beta plait domain superfamily (homologous_superfamily) [261-347]
- IPR033102: Negative elongation factor E (family) [214-375]
- IPR000504: RNA recognition motif domain (domain) [266-336]
Interaction partners: - Negative elongation factor B
- Negative elongation factor A
- Negative elongation factor D
- Transcription elongation factor SPT5
- Transcription elongation factor SPT4-A
- DNA-directed RNA polymerase II subunit RPB1
- Nuclear cap-binding protein subunit 1
- DNA-directed RNA polymerase II subunit RPB3
- DNA-directed RNA polymerases I, II, and III subunit RPABC2
- Nuclear cap-binding protein subunit 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003682 (chromatin binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0050793 (regulation of developmental process), GO:0051094 (positive regulation of developmental process), GO:0050794 (regulation of cellular process), GO:0048522 (positive regulation of cellular process), GO:0045597 (positive regulation of cell differentiation), GO:0045595 (regulation of cell differentiation), GO:1901532 (regulation of hematopoietic progenitor cell differentiation), GO:2000738 (positive regulation of stem cell differentiation), GO:1901534 (positive regulation of hematopoietic progenitor cell differentiation), GO:2000736 (regulation of stem cell differentiation), GO:1902036 (regulation of hematopoietic stem cell differentiation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)