wanglab/bioreason-pro-rl-reasoning-data huggingface dataset:
Protein: Calcium uniporter protein, mitochondrial (DdMCU) (Q54LT0)
Organism: Dictyostelium discoideum (Social amoeba)
Function: Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes a pore-forming and calcium-conducting subunit. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways.
Subcellular location: Mitochondrion inner membrane {ECO:0000269|PubMed:24889638}; Multi-pass membrane protein {ECO:0000255}.
Sequence: MNSFVIRNGFGLVRTFNTRLFTTSTQNLEGELKTILGQAKVSKLQEKLKLDPRSKITFNDFKGIAKEVGIEEKEINSVSNALAQSGSIIYLPNSLNENLKTSVFTKPAHIYQSLEHILDIENKGVGLNKLIESKKSEINSLRQKIQPLEEKKQVIDRKAHRRATAIIWTGLGYCFAQAAILARLTWWDLSWDIIEPVSYFLTFGSVLIGYTYFTMTKTEFTYEALNHRLFSKRQDKLFKRNNFPKEDYENLVQAIDKKEKELKELELATKYDHTH
InterPro domains: - IPR039055: MCU family (family) [28-267]
- IPR006769: Calcium uniporter protein, C-terminal (domain) [74-251]
Interaction partners: - EF-hand domain-containing protein
- Inositol 1,4,5-trisphosphate receptor-like protein A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0051179 (localization), GO:0051234 (establishment of localization), GO:0055085 (transmembrane transport), GO:1990542 (mitochondrial transmembrane transport), GO:0034220 (monoatomic ion transmembrane transport), GO:0098660 (inorganic ion transmembrane transport), GO:0006810 (transport), GO:0098655 (monoatomic cation transmembrane transport), GO:0006851 (mitochondrial calcium ion transmembrane transport), GO:0071702 (organic substance transport), GO:0098662 (inorganic cation transmembrane transport), GO:0006811 (monoatomic ion transport), GO:0070588 (calcium ion transmembrane transport), GO:0006816 (calcium ion transport), GO:0006812 (monoatomic cation transport), GO:0030001 (metal ion transport), GO:0051560 (mitochondrial calcium ion homeostasis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098798 (mitochondrial protein-containing complex), GO:0016020 (membrane), GO:0098796 (membrane protein complex), GO:1990351 (transporter complex), GO:0031975 (envelope), GO:0005737 (cytoplasm), GO:0031090 (organelle membrane), GO:0031967 (organelle envelope), GO:0043229 (intracellular organelle), GO:0098800 (inner mitochondrial membrane protein complex), GO:0005739 (mitochondrion), GO:1902495 (transmembrane transporter complex), GO:0043227 (membrane-bounded organelle), GO:0005740 (mitochondrial envelope), GO:0043231 (intracellular membrane-bounded organelle), GO:0034702 (ion channel complex), GO:0019866 (organelle inner membrane), GO:0031966 (mitochondrial membrane), GO:0005743 (mitochondrial inner membrane), GO:0034703 (cation channel complex), GO:0034704 (calcium channel complex)
Protein: Paired box 10 (V5ZE41)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Not known
Subcellular location: Nucleus {ECO:0000256|ARBA:ARBA00004123, ECO:0000256|PROSITE-ProRule:PRU00108, ECO:0000256|RuleBase:RU000682}.
Sequence: MYDNLTMLNLQNSSNWSGPNNWYHPADTITPQHGTDGCVEDLNVQMAGAGEGVVQGERDEVDDSQLHLQLKRKLQRNRTSFTQEQIDALEKEFERTHYPDVFARERLAAKIDLPEARIQVWFSNRRAKWRREEKLRNQRRSGSSSSCSQTHTPLSTSFNSPVYHSHHSNSSGSMHSRSDSSLSGYSSLSVFSSMQSLPSQSTPTYSCMLPPSPSALPPLSRKFDSSSYTSPHLPPPPTASANTGFFAGVSAAGQTAVQGGDQELGQGLSQYWTRLQ
InterPro domains: - IPR001356: Homeodomain (domain) [72-136]
- IPR009057: Homedomain-like superfamily (homologous_superfamily) [62-133]
- IPR017970: Homeobox, conserved site (conserved_site) [107-130]
- IPR050649: Paired Homeobox Transcription Factors (family) [62-229]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:0097159 (organic cyclic compound binding), GO:0003700 (DNA-binding transcription factor activity), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0003677 (DNA binding), GO:0043565 (sequence-specific DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0060041 (retina development in camera-type eye), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0048880 (sensory system development), GO:0007423 (sensory organ development), GO:0001654 (eye development), GO:0150063 (visual system development), GO:0043010 (camera-type eye development)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)
Protein: Candida glabrata strain CBS138 chromosome C complete sequence (Q6FWP8)
Organism: Candida glabrata (strain ATCC 2001 / BCRC 20586 / JCM 3761 / NBRC 0622 / NRRL Y-65 / CBS 138) (Yeast) (Nakaseomyces glabratus)
Function: Not known
Subcellular location: Membrane {ECO:0000256|ARBA:ARBA00004606}; Single-pass type II membrane protein {ECO:0000256|ARBA:ARBA00004606}.
Sequence: MSLLARYLLQRRVKRYSVLFVLFVGLFIFIARHLTNEEESDNGIYKDDKFPYEDGLLHSSSNSAYTFITDAMNKLTKKKKNSFLSPKGLSYFKDDERILKNWNTSSRVDKCKWLIRGIYADPNWNNRDILIGSDETNDKNKFHLSSERIRIHNYCFMKERIDPVQMFNELNISSTDAYDFQGRMFMFLRNVTSTDEAYIYPIIKNVRNGQVTTKPHTKLSPMQYNSNFMAYWKQESKGRGIALTAAPKDVKLLRSLFKVFEKLGNTYPIQIVQKGGEMSPTMESLIKDYAIETNQNVFIVDLSPVLDPDFATDHIRKFQNKWFASIFNTFEEVMLIDADVVIYTEPESFFNLDGYKNTGLLLWRDREIVDAKTHERCTDMAPYFEPALEEHRLLGTVQKYRLTGPSLETHDEPEAETLFDFFHRRIVTIIDSGVVIYNKKQKLHGLILAEYFHNSKQFAGCVYGDKEYFEFGALVAGEDYHVEAEKAVAIGAVGYHPPREKYYVCSGQMGHVDRNNNLLWSNGGLKNCKSDCAEADFEKMPKYFKAKYGTLKAVKEYYNGLMDIQGFVLPDVQKQKWVQYPDCQQFLYCAFITDPPEPDNKGKLVRFSDEEKMRYNEIGKAWYSGAND
InterPro domains: - IPR022751: Alpha-mannosyltransferase (family) [238-513]
- IPR029044: Nucleotide-diphospho-sugar transferases (homologous_superfamily) [236-473]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016757 (glycosyltransferase activity), GO:0016758 (hexosyltransferase activity), GO:0000030 (mannosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0070085 (glycosylation), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0043413 (macromolecule glycosylation), GO:1901135 (carbohydrate derivative metabolic process), GO:0036211 (protein modification process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0006486 (protein glycosylation), GO:0043412 (macromolecule modification), GO:0009100 (glycoprotein metabolic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0009101 (glycoprotein biosynthetic process), GO:0006493 (protein O-linked glycosylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0030312 (external encapsulating structure), GO:0005576 (extracellular region), GO:0031012 (extracellular matrix), GO:0062039 (biofilm matrix), GO:0062040 (fungal biofilm matrix)
Protein: Apical junction molecule (H2KYC5)
Organism: Caenorhabditis elegans
Function: Not known
Sequence: MDIENLQSSNNRSQKSRPPDGFNAILQTAFSAILAFVLSLRIIVFATARKRLTTTAANPDIQVEEASSSSSDTSSDTEIVEEGVFIQRQGEPTMSEKKPAQGMPSVMSLLQTDEILSSCSDDEPTTSGLIPLPSLIQSNMSVLPNFLHTINEEESDEIRSLTGSSLASPRTVVDRRLSNISLLSPTPDEDFGLEQQEQQPENSEEVRGRESRTSLEEERIVVGEPRTTRQIEITQSASEDDRLDLLKELAKIGKSNTSTSQMSTTTPEKPEEIIDATGTSDTAEKIEVVISKEEPAEQKNEVEEPDYAQVPAESEDDAAQELAPTDSAIQVVHVLEAPQKAELVTIPLAHSEAEDHKLADADRDQEEELVFNEERRKTVTMDDNASLRSASITFDANRDEQDLLNESFASNPTDETVLMQKTKDEMDATSERPRSPLDLPPPPASVVLHPSAPPPPPPPLSSTEVVGNTTTTTTTHYAPKTWNDPSITTAPKIPVSLLSGAQPLPSVLTQRTTTSSYSAPNYSASSMSGVPSDVAPPPPLPIPNQSSSSAASYQHHHASSISKSISSSREDLLSEHATSRSTVREIPVHRAPSTAPSHSSVFEYHMMPTTTSTYHHVETPSDEYYRREVMTRTIITRSTEALSQTPLGRPASPLDRYLPYPTTTTTTSGDGRTREEKTVDYKVTYHRDIEEQERRIREDQARRQQEEQDRRDREDNARRILAQREHQEMERLREQQNLSERALAERERADKERLQQERLLRQQREKKRREEWDRLESIRLAEEEAELARRRALEKERIDREKAEEERKTMERLERERARLERERLEEERRQKEKAETERIERERREHERIEIERIERIKRERIERERREREEKKAEEDRLLRERLELERIERERRELEARERQELELQRREAEDRERQRLEDEAREMRRREEERREAELVADVHRQAEERERLRKRQEREEAERLERIRLEQQKIDMERIDAERRERERKEEERREFELIEAARRKKEARDRDRLDEMERERVREEEERREKERREQERRIAAEKERKRRQEEEEEIARLNELQRAAAARQAQRNAELDRQRQRDELDRKAQELSEREMREKERRDRERANEEAQLADLLERERHNQLIRENERREAVERANNRRLEDRRSRDKLDHIVRERSEKEQFELEKRRLLAEKEAMNRKKNHLLSSETLAKLTQPMYYTTREPEVTTKVERQVIERIDRNVWVEDVPYAPSQSAMGYLDNDENNRDRLYNPNDLNRNGSSRSRYQRAKNEKARRDFYHSSQDSAGLA
InterPro domains: - IPR038825: Apical junction component (family) [27-1289]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0008092 (cytoskeletal protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0071840 (cellular component organization or biogenesis), GO:0007275 (multicellular organism development), GO:0009790 (embryo development), GO:0016043 (cellular component organization), GO:0034330 (cell junction organization), GO:0009792 (embryo development ending in birth or egg hatching), GO:0045216 (cell-cell junction organization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0030054 (cell junction), GO:0005886 (plasma membrane), GO:0070161 (anchoring junction), GO:0098590 (plasma membrane region), GO:0016327 (apicolateral plasma membrane), GO:0005911 (cell-cell junction), GO:0043296 (apical junction complex), GO:0005912 (adherens junction)
Protein: DNA-binding protein (P71859)
Organism: Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Function: Not known
Sequence: MTGVSDIQEAVAQIKAAGPSKPRLARDPVNQPMINNWVEAIGDRNPIYVDDAAARAAGHPGIVAPPAMIQVWTMMGLGGVRPKDDPLGPIIKLFDDAGYIGVVATNCEQTYHRYLLPGEQVSISAELGDVVGPKQTALGEGWFINQHIVWQVGDEDVAEMNWRILKFKPAGSPSSVPDDLDPDAMMRPSSSRDTAFFWDGVKAHELRIQRLADGSLRHPPVPAVWQDKSVPINYVVSSGRGTVFSFVVHHAPKVPGRTVPFVIALVELEEGVRMLGELRGADPARVAIGMPVRATYIDFPDWSLYAWEPDE
InterPro domains: - IPR029069: HotDog domain superfamily (homologous_superfamily) [23-174]
- IPR002878: ChsH2, C-terminal OB-fold domain (domain) [234-295]
- IPR039569: FAS1-like, dehydratase domain region (domain) [26-159]
- IPR012340: Nucleic acid-binding, OB-fold (homologous_superfamily) [182-303]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0016790 (thiolester hydrolase activity), GO:0016289 (CoA hydrolase activity), GO:0047617 (acyl-CoA hydrolase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0006629 (lipid metabolic process), GO:0044282 (small molecule catabolic process), GO:1901575 (organic substance catabolic process), GO:0044248 (cellular catabolic process), GO:0006082 (organic acid metabolic process), GO:0030258 (lipid modification), GO:0006631 (fatty acid metabolic process), GO:0016054 (organic acid catabolic process), GO:0044242 (cellular lipid catabolic process), GO:0043436 (oxoacid metabolic process), GO:0016042 (lipid catabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0046395 (carboxylic acid catabolic process), GO:0034440 (lipid oxidation), GO:0019395 (fatty acid oxidation), GO:0009062 (fatty acid catabolic process), GO:0072329 (monocarboxylic acid catabolic process), GO:0006635 (fatty acid beta-oxidation), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)
Protein: Inactive leucine-rich repeat receptor-like serine/threonine-protein kinase At5g24100 (Q9FL63)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Not known
Subcellular location: Cell membrane {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}.
Sequence: MSRGRSFIFYFVLFLFFGSSALYSQVTGDLAGDRQALLDFLNNIIHPRSLAWNTSSPVCTTWPGVTCDIDGTRVTALHLPGASLLGVIPPGTISRLSELQILSLRSNGLRGPFPIDFLQLKKLKAISLGNNRFSGPLPSDYATWTNLTVLDLYSNRFNGSIPAGFANLTGLVSLNLAKNSFSGEIPDLNLPGLRRLNFSNNNLTGSIPNSLKRFGNSAFSGNNLVFENAPPPAVVSFKEQKKNGIYISEPAILGIAISVCFVIFFVIAVVIIVCYVKRQRKSETEPKPDKLKLAKKMPSEKEVSKLGKEKNIEDMEDKSEINKVMFFEGSNLAFNLEDLLIASAEFLGKGVFGMTYKAVLEDSKVIAVKRLKDIVVSRKDFKHQMEIVGNIKHENVAPLRAYVCSKEEKLMVYDYDSNGSLSLRLHGKNADEGHVPLNWETRLRFMIGVAKGLGHIHTQNLAHGNIKSSNVFMNSEGYGCISEAGLPLLTNPVVRADSSARSVLRYRAPEVTDTRRSTPESDIYSFGILMLETLTGRSIMDDRKEGIDLVVWVNDVISKQWTGEVFDLELVKTPNVEAKLLQMLQLGTSCTAMVPAKRPDMVKVVETLEEIERD
InterPro domains: - IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [344-610]
- IPR032675: Leucine-rich repeat domain superfamily (homologous_superfamily) [32-231]
- IPR013210: Leucine-rich repeat-containing N-terminal, plant-type (domain) [31-68]
- IPR001245: Serine-threonine/tyrosine-protein kinase, catalytic domain (domain) [344-608]
- IPR001611: Leucine-rich repeat (repeat) [73-213]
- IPR017441: Protein kinase, ATP binding site (binding_site) [347-369]
- IPR000719: Protein kinase domain (domain) [341-611]
- IPR050994: Arabidopsis Inactive Receptor-like Kinases (family) [9-611]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0040007 (growth), GO:0048589 (developmental growth), GO:0048856 (anatomical structure development), GO:0071840 (cellular component organization or biogenesis), GO:0009653 (anatomical structure morphogenesis), GO:0016049 (cell growth), GO:0071554 (cell wall organization or biogenesis), GO:0000902 (cell morphogenesis), GO:0016043 (cellular component organization), GO:0071669 (plant-type cell wall organization or biogenesis), GO:0071555 (cell wall organization), GO:0060560 (developmental growth involved in morphogenesis), GO:0009826 (unidimensional cell growth), GO:0045229 (external encapsulating structure organization), GO:0009664 (plant-type cell wall organization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)
Protein: Triplex capsid protein 1 (F5HA93)
Organism: Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5)
Function: Structural component of the T=16 icosahedral capsid. The capsid is composed of pentamers and hexamers of major capsid protein/MCP, which are linked together by heterotrimers called triplexes. These triplexes are formed by a single molecule of triplex protein 1/TRX1 and two copies of triplex protein 2/TRX2. Additionally, TRX1 is required for efficient transport of TRX2 to the nucleus, which is the site of capsid assembly.
Subcellular location: Virion {ECO:0000255|HAMAP-Rule:MF_04018}. Host nucleus {ECO:0000255|HAMAP-Rule:MF_04018}.
Sequence: MDARAVAKRPRDPADEDNELVTALKAKREVNTISVRYLYHADHQALTARFFVPEGLVEFEAQPGALLIRMETGCDSPRHLYISLYLLGIRASNVSASTRCLLESVYTASAARAALQWLDLGPHLLHRRLETLGCVKTVSLGITSLLTCVMRGYLYNTLKTEVFALMIPKDMYLTWEETRGRLQYVYLIIVYDYDGPETRPGIYVLTSSIAHWQTLVDVARGKFARERCSFVNRRITRPRQIPLCTGVIQKLGWCLADDIHTSFLVHKELKLSVVRLDNFSVELGDFREFV
InterPro domains: - IPR004999: Herpesvirus capsid shell protein 1 (family) [4-290]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0016032 (viral process), GO:0019068 (virion assembly), GO:0019058 (viral life cycle)
Cellular Component (CC): GO:0005575 (cellular component), GO:0044423 (virion component), GO:0019033 (viral tegument)
Protein: Variant-surface-glycoprotein phospholipase C (VSG lipase) (EC 4.6.1.14) (Glycosylphosphatidylinositol-specific phospholipase C) (GPI-PLC) (P09194)
Organism: Trypanosoma brucei brucei
Function: By hydrolysis of the attached glycolipid, releases soluble variant surface glycoprotein containing phosphoinositol from the cell wall of T.brucei after cell lysis. It also cleaves similar membrane anchors on some mammalian proteins. VSG lipase may play a role in processes such as parasite differentiation or antigenic variation.
Subcellular location: Membrane; Peripheral membrane protein.
Sequence: MFGGVKWSPQSWMSDTRSSIEKKCIGQVYMVGAHNAGTHGIQMFSPFGLDAPEKLRSLPPYVTFLLRFLTVGVSSRWGRCQNLSIRQLLDHGVRYLDLRMNISPDQENKIYTTHFHISVPLQEVLKDVKDFLTTPASANEFVILDFLHFYGFNESHTMKRFVEELQALEEFYIPTTVSLTTPLCNLWQSTRRIFLVVRPYVEYPYARLRSVALKSIWVNQMELNDLLDRLEELMTRDLEDVSIGGVPSKMYVTQAIGTPRNNDFAVAACCGACPGSHPDLYSAAKHKNPHLLKWFYDLNVNGVMRGERVTIRRGNNTHGNILLLDFVQEGTCTVKGVDKPMNAVALCVHLNTNQTARS
InterPro domains: - IPR000909: Phosphatidylinositol-specific phospholipase C, X domain (domain) [19-198]
- IPR017946: PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamily (homologous_superfamily) [8-351]
- IPR051057: Phosphoinositide phospholipase C domain-containing protein (family) [11-343]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0016298 (lipase activity), GO:0042578 (phosphoric ester hydrolase activity), GO:0008081 (phosphoric diester hydrolase activity), GO:0004620 (phospholipase activity), GO:0004629 (phospholipase C activity), GO:0004435 (phosphatidylinositol phospholipase C activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0043603 (amide metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006629 (lipid metabolic process), GO:0019637 (organophosphate metabolic process), GO:0006793 (phosphorus metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0046486 (glycerolipid metabolic process), GO:0006644 (phospholipid metabolic process), GO:0006650 (glycerophospholipid metabolic process), GO:0046488 (phosphatidylinositol metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0005737 (cytoplasm), GO:0120025 (plasma membrane bounded cell projection), GO:0099568 (cytoplasmic region), GO:0043227 (membrane-bounded organelle), GO:0005929 (cilium), GO:0032838 (plasma membrane bounded cell projection cytoplasm), GO:0097014 (ciliary plasm)
Protein: Subtilisin BPN' (EC 3.4.21.62) (Alkaline protease) (Subtilisin DFE) (Subtilisin Novo) (P00782)
Organism: Bacillus amyloliquefaciens (Bacillus velezensis)
Function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Has a high substrate specificity to fibrin.
Subcellular location: Secreted.
Sequence: MRGKKVWISLLFALALIFTMAFGSTSSAQAAGKSNGEKKYIVGFKQTMSTMSAAKKKDVISEKGGKVQKQFKYVDAASATLNEKAVKELKKDPSVAYVEEDHVAHAYAQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETNPFQDNNSHGTHVAGTVAALNNSIGVLGVAPSASLYAVKVLGADGSGQYSWIINGIEWAIANNMDVINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGTSGSSSTVGYPGKYPSVIAVGAVDSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPNWTNTQVRSSLENTTTKLGDSFYYGKGLINVQAAAQ
InterPro domains: - IPR054399: Fervidolysin-like, N-terminal prodomain (domain) [41-99]
- IPR034202: Subtilisin Carlsberg-like catalytic domain (domain) [132-360]
- IPR050131: Subtilisin-like serine protease (family) [32-370]
- IPR015500: Peptidase S8, subtilisin-related (family) [130-341]
- IPR023827: Peptidase S8, subtilisin, Asp-active site (active_site) [135-146]
- IPR022398: Peptidase S8, subtilisin, His-active site (active_site) [171-181]
- IPR023828: Peptidase S8, subtilisin, Ser-active site (active_site) [326-336]
- IPR000209: Peptidase S8/S53 domain (domain) [130-373]
- IPR036852: Peptidase S8/S53 domain superfamily (homologous_superfamily) [108-382]
- IPR037045: Peptidase S8 propeptide/proteinase inhibitor I9 superfamily (homologous_superfamily) [30-109]
Interaction partners: - Bacillolysin
- Extracellular metalloprotease.
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0017171 (serine hydrolase activity), GO:0008233 (peptidase activity), GO:0008236 (serine-type peptidase activity), GO:0004175 (endopeptidase activity), GO:0004252 (serine-type endopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0006508 (proteolysis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005576 (extracellular region), GO:0005615 (extracellular space)