Protein: Actin-related protein 2 (Actin-like protein 2) (A7MB62)
Organism: Bos taurus (Bovine)
Function: ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
Subcellular location: Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P61160}. Cell projection {ECO:0000250|UniProtKB:P61160}. Nucleus {ECO:0000250|UniProtKB:P61160}.
Sequence: MDSQGRKVVVCDNGTGFVKCGYAGSNFPEHIFPALVGRPIIRSTTKVGNIEIKDLMVGDEASELRSMLEVNYPMENGIVRNWDDMKHLWDYTFGPEKLNIDTRNCKILLTEPPMNPTKNREKIVEVMFETYQFSGVYVAIQAVLTLYAQGLLTGVVVDSGDGVTHICPVYEGFSLPHLTRRLDIAGRDITRYLIKLLLLRGYAFNHSADFETVRMIKEKLCYVGYNIEQEQKLALETTVLVESYTLPDGRIIKVGGERFEAPEALFQPHLINVEGVGVAELLFNTIQAADIDTRSEFYKHIVLSGGSTMYPGLPSRLERELKQLYLERVLKGDVEKLSKFKIRIEDPPRRKHMVFLGGAVLADIMKDKDNFWMTRQEYQEKGVRVLEKLGVTVR
InterPro domains: - IPR043129: ATPase, nucleotide binding domain (homologous_superfamily) [6-387]
- IPR004000: Actin family (family) [6-390]
- IPR020902: Actin/actin-like conserved site (conserved_site) [108-120]
Interaction partners: - Actin-related protein 3
- Actin-related protein 3
- Actin nucleation-promoting factor WASL
- Actin-related protein 2/3 complex subunit 3
- Actin-related protein 2/3 complex subunit 2
- Actin-related protein 2/3 complex subunit 5
- Actin-related protein 2/3 complex subunit 1B
- Actin nucleation-promoting factor WAS
- Actin-related protein 2/3 complex subunit 4
- Src substrate cortactin
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0002682 (regulation of immune system process), GO:0048870 (cell motility), GO:0040012 (regulation of locomotion), GO:0050794 (regulation of cellular process), GO:0048522 (positive regulation of cellular process), GO:2000145 (regulation of cell motility), GO:0002685 (regulation of leukocyte migration), GO:0016477 (cell migration), GO:2000147 (positive regulation of cell motility), GO:0002687 (positive regulation of leukocyte migration), GO:0030335 (positive regulation of cell migration), GO:0030334 (regulation of cell migration), GO:1902624 (positive regulation of neutrophil migration), GO:1902622 (regulation of neutrophil migration), GO:0014812 (muscle cell migration), GO:0090022 (regulation of neutrophil chemotaxis), GO:0071622 (regulation of granulocyte chemotaxis), GO:0090313 (regulation of protein targeting to membrane)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)