Protein: 4-hydroxyproline betaine 2-epimerase (Hyp-B 2-epimerase) (EC 5.1.1.22) ((4R)-4-hydroxyproline betaine 2-epimerase) (Q0FPQ4)
Organism: Salipiger bermudensis (strain DSM 26914 / JCM 13377 / KCTC 12554 / HTCC2601) (Pelagibaca bermudensis)
Function: Catalyzes the 2-epimerization of trans-4-hydroxy-L-proline betaine (tHyp-B) to cis-4-hydroxy-D-proline betaine (cHyp-B). Is involved in a catabolic pathway that degrades tHyp-B to alpha-ketoglutarate. This pathway would permit the utilization of tHyp-B as a carbon and nitrogen source in the absence of osmotic stress, since tHyp-B functions as an osmolyte and is not catabolized when it is needed as osmoprotectant. To a lesser extent, can also catalyze the racemization of L-proline betaine.
Sequence: MKIAEIQLFQHDLPVVNGPYRIASGDVWSLTTTIVKIIAEDGTIGWGETCPVGPTYAEAHAGGALAALEVLASGLAGAEALPLPLHTRMDSLLCGHNYAKSALDIAVHDLWGKRLGVPVHELLGGALTDSVSSYYSLGVMEPDEAARQALEKQREGYSRLQVKLGARPIEIDIEAIRKVWEAVRGTGIALAADGNRGWTTRDALRFSRECPDIPFVMEQPCNSFEDLEAIRPLCHHALYMDEDGTSLNTVITAAATSLVDGFGMKVSRIGGLQHMRAFRDFCAARNLPHTCDDAWGGDIVSAACTHIASTVLPRLMEGAWLAQPYVAEHYDAENGVRIEGGRIRVPQGPGLGLTIDPERFGPPLFSA
InterPro domains: - IPR034622: 4R-hydroxyproline betaine 2-epimerase (family) [1-366]
- IPR029065: Enolase C-terminal domain-like (domain) [145-358]
- IPR013341: Mandelate racemase/muconate lactonizing enzyme, N-terminal domain (domain) [14-124]
- IPR013342: Mandelate racemase/muconate lactonizing enzyme, C-terminal (domain) [142-237]
- IPR029017: Enolase-like, N-terminal (homologous_superfamily) [1-356]
- IPR034593: D-galactonate dehydratase DgoD-like (family) [1-358]
- IPR036849: Enolase-like, C-terminal domain superfamily (homologous_superfamily) [112-360]
Interaction partners: - Possible dioxygenase reductase subunit
- Probable dienelactone hydrolase protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016853 (isomerase activity), GO:0016854 (racemase and epimerase activity), GO:0016855 (racemase and epimerase activity, acting on amino acids and derivatives), GO:0036361 (racemase activity, acting on amino acids and derivatives)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006575 (cellular modified amino acid metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0044248 (cellular catabolic process), GO:0006577 (amino-acid betaine metabolic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0042219 (cellular modified amino acid catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0006579 (amino-acid betaine catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0009274 (peptidoglycan-based cell wall)