Protein: Nondiscriminating glutamyl-tRNA synthetase EARS2, mitochondrial (EC 6.1.1.24) (Glutamate--tRNA(Gln) ligase EARS2, mitochondrial) (EC 6.1.1.17) (Glutamyl-tRNA synthetase) (GluRS) (Mitochondrial glutamyl-tRNA synthetase) (mtGluRS) (Q5JPH6)
Organism: Homo sapiens (Human)
Function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).
Subcellular location: Mitochondrion matrix {ECO:0000269|PubMed:19805282}.
Sequence: MAALLRRLLQRERPSAASGRPVGRREANLGTDAGVAVRVRFAPSPTGFLHLGGLRTALYNYIFAKKYQGSFILRLEDTDQTRVVPGAAENIEDMLEWAGIPPDESPRRGGPAGPYQQSQRLELYAQATEALLKTGAAYPCFCSPQRLELLKKEALRNHQTPRYDNRCRNMSQEQVAQKLAKDPKPAIRFRLEQVVPAFQDLVYGWNRHEVASVEGDPVIMKSDGFPTYHLACVVDDHHMGISHVLRGSEWLVSTAKHLLLYQALGWQPPHFAHLPLLLNRDGSKLSKRQGDVFLEHFAADGFLPDSLLDIITNCGSGFAENQMGRTLPELITQFNLTQVTCHSALLDLEKLPEFNRLHLQRLVSNESQRRQLVGKLQVLVEEAFGCQLQNRDVLNPVYVERILLLRQGHICRLQDLVSPVYSYLWTRPAVGRAQLDAISEKVDVIAKRVLGLLERSSMSLTQDMLNGELKKLSEGLEGTKYSNVMKLLRMALSGQQQGPPVAEMMLALGPKEVRERIQKVVSS
InterPro domains: - IPR049940: Glutamyl-Q tRNA(Asp) synthetase/Glutamate--tRNA ligase (family) [31-520]
- IPR020058: Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain (domain) [37-351]
- IPR008925: Aminoacyl-tRNA synthetase, class I, anticodon-binding superfamily (homologous_superfamily) [329-519]
- IPR000924: Glutamyl/glutaminyl-tRNA synthetase (family) [40-250]
- IPR033910: Glutamyl-tRNA synthetase (domain) [36-361]
- IPR045462: Aminoacyl-tRNA synthetase, class I, anticodon-binding (domain) [392-520]
- IPR001412: Aminoacyl-tRNA synthetase, class I, conserved site (conserved_site) [43-54]
- IPR004527: Glutamate-tRNA ligase, bacterial/mitochondrial (family) [36-521]
- IPR014729: Rossmann-like alpha/beta/alpha sandwich fold (homologous_superfamily) [30-362]
- IPR020751: Aminoacyl-tRNA synthetase, class I, anticodon-binding domain, subdomain 2 (homologous_superfamily) [432-523]
Interaction partners: - Ubiquitin-like protein FUBI
- Methionine--tRNA ligase, cytoplasmic
- Methionine--tRNA ligase, mitochondrial
- Bifunctional glutamate/proline--tRNA ligase
- Leucine--tRNA ligase, mitochondrial
- Glutamyl-tRNA
- Probable proline--tRNA ligase, mitochondrial
- Isoleucine--tRNA ligase, mitochondrial
- Aspartate--tRNA ligase, mitochondrial
- Lysine--tRNA ligase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006082 (organic acid metabolic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006412 (translation), GO:0009059 (macromolecule biosynthetic process), GO:0006518 (peptide metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0043038 (amino acid activation), GO:0010467 (gene expression), GO:0019752 (carboxylic acid metabolic process), GO:0016070 (RNA metabolic process), GO:0043043 (peptide biosynthetic process), GO:0006418 (tRNA aminoacylation for protein translation), GO:0043039 (tRNA aminoacylation), GO:0034660 (ncRNA metabolic process), GO:0006399 (tRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)