Protein: Endoribonuclease LACTB2 (EC 3.1.27.-) (Beta-lactamase-like protein 2) (Q53H82)
Organism: Homo sapiens (Human)
Function: Endoribonuclease; cleaves preferentially 3' to purine-pyrimidine dinucleotide motifs in single-stranded RNA. The cleavage product contains a free 3' -OH group. Has no activity with double-stranded RNA or DNA. Required for normal mitochondrial function and cell viability.
Subcellular location: Mitochondrion matrix {ECO:0000269|PubMed:26826708}.
Sequence: MAAVLQRVERLSNRVVRVLGCNPGPMTLQGTNTYLVGTGPRRILIDTGEPAIPEYISCLKQALTEFNTAIQEIVVTHWHRDHSGGIGDICKSINNDTTYCIKKLPRNPQREEIIGNGEQQYVYLKDGDVIKTEGATLRVLYTPGHTDDHMALLLEEENAIFSGDCILGEGTTVFEDLYDYMNSLKELLKIKADIIYPGHGPVIHNAEAKIQQYISHRNIREQQILTLFRENFEKSFTVMELVKIIYKNTPENLHEMAKHNLLLHLKKLEKEGKIFSNTDPDKKWKAHL
InterPro domains: - IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [205-288]
- IPR001279: Metallo-beta-lactamase (domain) [29-199]
- IPR036866: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like (homologous_superfamily) [1-274]
- IPR047921: LACTB2-like, MBL-fold metallo hydrolase domain (domain) [14-203]
- IPR050662: Secondary metabolite biosynthesis-associated thioesterase (family) [3-278]
- IPR041516: LACTB2, winged helix domain (domain) [237-284]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0043167 (ion binding), GO:0005515 (protein binding), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0046983 (protein dimerization activity), GO:0042802 (identical protein binding), GO:0043169 (cation binding), GO:0042803 (protein homodimerization activity), GO:0004518 (nuclease activity), GO:0046872 (metal ion binding), GO:0004540 (ribonuclease activity), GO:0046914 (transition metal ion binding), GO:0004519 (endonuclease activity), GO:0008270 (zinc ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006520 (amino acid metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006790 (sulfur compound metabolic process), GO:0006082 (organic acid metabolic process), GO:0000096 (sulfur amino acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0006555 (methionine metabolic process), GO:0009066 (aspartate family amino acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)