Protein: Protein phosphatase methylesterase 1 (PME-1) (EC 3.1.1.89) (Q8BVQ5)
Organism: Mus musculus (Mouse)
Function: Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme (By similarity).
Sequence: MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFTPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRGHGETKVKNSEDLSAETMAKDVGNVVEAMYGDLPPPVMLIGHSMGGAIAVHTAAANLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESVNKRKKEDDMETKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC
InterPro domains: - IPR029058: Alpha/Beta hydrolase fold (homologous_superfamily) [39-386]
- IPR000639: Epoxide hydrolase-like (family) [104-366]
- IPR000073: Alpha/beta hydrolase fold-1 (domain) [79-362]
- IPR016812: Protein phosphatase methylesterase, eukaryotic (family) [1-383]
Interaction partners: - Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
- Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
- Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
- Leucine carboxyl methyltransferase 1
- Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform
- Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit
- Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
- Serine/threonine-protein phosphatase 2A activator
- Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
- Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0005488 (binding), GO:0030234 (enzyme regulator activity), GO:0005515 (protein binding), GO:0140678 (molecular function inhibitor activity), GO:0019899 (enzyme binding), GO:0004857 (enzyme inhibitor activity), GO:0019208 (phosphatase regulator activity), GO:0019900 (kinase binding), GO:0019888 (protein phosphatase regulator activity), GO:0019212 (phosphatase inhibitor activity), GO:0004864 (protein phosphatase inhibitor activity), GO:0019901 (protein kinase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0071704 (organic substance metabolic process), GO:0022402 (cell cycle process), GO:0044238 (primary metabolic process), GO:0070988 (demethylation), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:1901564 (organonitrogen compound metabolic process), GO:1903047 (mitotic cell cycle process), GO:0006482 (protein demethylation), GO:0019538 (protein metabolic process), GO:0043170 (macromolecule metabolic process), GO:0000278 (mitotic cell cycle), GO:0044770 (cell cycle phase transition), GO:0036211 (protein modification process), GO:0044772 (mitotic cell cycle phase transition), GO:0043412 (macromolecule modification), GO:0044839 (cell cycle G2/M phase transition), GO:0000086 (G2/M transition of mitotic cell cycle), GO:0008214 (protein dealkylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043229 (intracellular organelle), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)