Protein: Diaminopimelate epimerase, chloroplastic (DAP epimerase) (EC 5.1.1.7) (Q9LFG2)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Racemase that operates by a 'two-base' mechanism, which involves one active-site cysteine acting as a base to abstract the alpha-proton of an amino acid, while a second cysteine thiol functions as an acid to reprotonate the resulting planar carbanionic intermediate from the opposite face.
Subcellular location: Plastid, chloroplast {ECO:0000305}.
Sequence: MEIAAVSTVSVAPQSRRVSNAFSRNLGSVSSLSFGFFEKEYCFKSPSLRVSAAASMDAVTAEKFSPASFLDKKETGVLHFVKYHGLGNDFILVDNRDSSEPKITQEQAAKLCDRNFGVGADGVIFAMPGVNGTDYAMRIFNSDGSEPEMCGNGVRCFARFIAELENLQGKHSFTIHTGAGLIVPEIQDDGQVKVDMGTPILKAQDVPTKLSGNKGEAVVEAELVVDGVSWNVTCVSMGNPHCITFGKKGGPNLKVDDLNLPEIGPKFEHHEMFPARTNTEFVEVLSRSHLKMRVWERGAGATLACGTGACALVVAAVLEGRADRKCTVDLPGGPLEIEWKQEDNHIYMTGPAEAVFYGSALL
InterPro domains: - IPR001653: Diaminopimelate epimerase, DapF (family) [65-362]
- IPR018510: Diaminopimelate epimerase, active site (active_site) [141-155]
Interaction partners: - Diaminopimelate decarboxylase 2, chloroplastic
- Diaminopimelate decarboxylase 1, chloroplastic
- LL-diaminopimelate aminotransferase, chloroplastic
- aspartate-semialdehyde dehydrogenase
- Carbamoyl phosphate synthase arginine-specific large chain, chloroplastic
- Aminotransferase ALD1, chloroplastic
- Aspartokinase 1, chloroplastic
- Aspartokinase 3, chloroplastic
- Aspartokinase 2, chloroplastic
- Probable N-acetyl-gamma-glutamyl-phosphate reductase, chloroplastic
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016853 (isomerase activity), GO:0016854 (racemase and epimerase activity), GO:0016855 (racemase and epimerase activity, acting on amino acids and derivatives), GO:0036361 (racemase activity, acting on amino acids and derivatives), GO:0047661 (amino-acid racemase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0008652 (amino acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0016053 (organic acid biosynthetic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0009066 (aspartate family amino acid metabolic process), GO:0009067 (aspartate family amino acid biosynthetic process), GO:0006553 (lysine metabolic process), GO:0043648 (dicarboxylic acid metabolic process), GO:0009085 (lysine biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle)