Protein: Negative elongation factor E (P19426)
Organism: Mus musculus (Mouse)
Function: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (By similarity)
Subcellular location: Nucleus. Chromosome. Note=Localizes to chromatin. Phosphorylation by the P-TEFb complex promotes its release from chromatin
Sequence: MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSGPASQGGVKRSLSEQPVVDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSMSADEDLQEPSRRPQRKSLYESFVSSSDRLRELGQDGEEAEAPGAGDGPPRGFDWSYEEHGSARSSASPPRSRSRDRSHDRSRDRDRDKERDRDRDRDRDRDRDKDKDRDRDRDRDKERDRDRDRDRDRERDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDL
InterPro domains: - IPR035979: RNA-binding domain superfamily (homologous_superfamily) [207-334]
- IPR034637: Negative elongation factor E, RNA recognition motif (domain) [262-336]
- IPR033102: Negative elongation factor E (family) [214-375]
- IPR000504: RNA recognition motif domain (domain) [266-336]
- IPR012677: Nucleotide-binding alpha-beta plait domain superfamily (homologous_superfamily) [261-347]
Interaction partners: - Negative elongation factor B
- Negative elongation factor A
- Negative elongation factor D
- Transcription elongation factor SPT5
- Transcription elongation factor SPT4-A
- Transcription elongation factor SPT4
- DNA-directed RNA polymerase II subunit RPB1
- Nuclear cap-binding protein subunit 1
- DNA-directed RNA polymerase II subunit RPB3
- DNA-directed RNA polymerases I, II, and III subunit RPABC2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003682 (chromatin binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0019222 (regulation of metabolic process), GO:0051240 (positive regulation of multicellular organismal process), GO:0006807 (nitrogen compound metabolic process), GO:0051239 (regulation of multicellular organismal process), GO:0071704 (organic substance metabolic process), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0050793 (regulation of developmental process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0051094 (positive regulation of developmental process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0045597 (positive regulation of cell differentiation), GO:0001824 (blastocyst development), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0030154 (cell differentiation), GO:0046483 (heterocycle metabolic process), GO:0001825 (blastocyst formation), GO:0060255 (regulation of macromolecule metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0001825 (blastocyst formation), GO:0043170 (macromolecule metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:2000026 (regulation of multicellular organismal development), GO:0006139 (nucleobase-containing compound metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0009790 (embryo development), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0051962 (positive regulation of nervous system development), GO:0044260 (cellular macromolecule metabolic process), GO:0010562 (positive regulation of phosphorus metabolic process), GO:0051246 (regulation of protein metabolic process), GO:0060284 (regulation of cell development), GO:0010720 (positive regulation of cell development), GO:0050769 (positive regulation of neurogenesis), GO:0051960 (regulation of nervous system development), GO:0010468 (regulation of gene expression), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0048863 (stem cell differentiation), GO:0051254 (positive regulation of RNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0045995 (regulation of embryonic development), GO:0051247 (positive regulation of protein metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0006259 (DNA metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:0010467 (gene expression), GO:0051174 (regulation of phosphorus metabolic process), GO:0009792 (embryo development ending in birth or egg hatching), GO:0043009 (chordate embryonic development), GO:0016070 (RNA metabolic process), GO:0031399 (regulation of protein modification process), GO:0045937 (positive regulation of phosphate metabolic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0006396 (RNA processing), GO:0019220 (regulation of phosphate metabolic process), GO:0050767 (regulation of neurogenesis), GO:0031401 (positive regulation of protein modification process), GO:0042325 (regulation of phosphorylation), GO:0001932 (regulation of protein phosphorylation), GO:0001701 (in utero embryonic development), GO:0033674 (positive regulation of kinase activity), GO:0006364 (rRNA processing), GO:0042327 (positive regulation of phosphorylation), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0001934 (positive regulation of protein phosphorylation), GO:0034660 (ncRNA metabolic process), GO:0006302 (double-strand break repair), GO:0071900 (regulation of protein serine/threonine kinase activity), GO:0016073 (snRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)