Protein: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase (3-beta-HSD) [Includes: 3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC 1.1.1.145) (3-beta-hydroxy-5-ene steroid dehydrogenase) (Progesterone reductase); Steroid Delta-isomerase (EC 5.3.3.1) (Delta-5-3-ketosteroid isomerase)] (P14893)
Organism: Bos taurus (Bovine)
Function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Subcellular location: Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
Sequence: MAGWSCLVTGGGGFLGQRIICLLVEEKDLQEIRVLDKVFRPEVREEFSKLQSKIKLTLLEGDILDEQCLKGACQGTSVVIHTASVIDVRNAVPRETIMNVNVKGTQLLLEACVQASVPVFIHTSTIEVAGPNSYREIIQDGREEEHHESAWSSPYPYSKKLAEKAVLGANGWALKNGGTLYTCALRPMYIYGEGSPFLSAYMHGALNNNGILTNHCKFSRVNPVYVGNVAWAHILALRALRDPKKVPNIQGQFYYISDDTPHQSYDDLNYTLSKEWGFCLDSRMSLPISLQYWLAFLLEIVSFLLSPIYKYNPCFNRHLVTLSNSVFTFSYKKAQRDLGYEPLYTWEEAKQKTKEWIGSLVKQHKETLKTKIH
InterPro domains: - IPR002225: 3-beta hydroxysteroid dehydrogenase/isomerase (domain) [7-287]
- IPR036291: NAD(P)-binding domain superfamily (homologous_superfamily) [1-357]
- IPR050177: Bifunctional lipid A modification and metabolic enzymes (family) [5-356]
Interaction partners: - Cholesterol side-chain cleavage enzyme, mitochondrial
- Steroid 17-alpha-hydroxylase/17,20 lyase
- Aromatase
- Cytochrome P450 11B1, mitochondrial
- Steroid 21-hydroxylase
- 17beta-estradiol 17-dehydrogenase
- Cytochrome P450 family 7 subfamily B member 1
- 3-oxo-5-alpha-steroid 4-dehydrogenase 1
- 17-beta hydroxysteroid dehydrogenase 2
- 3-oxo-5-alpha-steroid 4-dehydrogenase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016614 (oxidoreductase activity, acting on CH-OH group of donors), GO:0016229 (steroid dehydrogenase activity), GO:0016616 (oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor), GO:0033764 (steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0065008 (regulation of biological quality), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0008207 (C21-steroid hormone metabolic process), GO:0044281 (small molecule metabolic process), GO:0042445 (hormone metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0042180 (cellular ketone metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0120254 (olefinic compound metabolic process), GO:0006082 (organic acid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006700 (C21-steroid hormone biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0120255 (olefinic compound biosynthetic process), GO:0008202 (steroid metabolic process), GO:0042181 (ketone biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0006694 (steroid biosynthetic process), GO:0120178 (steroid hormone biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0031090 (organelle membrane), GO:0031967 (organelle envelope), GO:0005739 (mitochondrion), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0005740 (mitochondrial envelope), GO:0019866 (organelle inner membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0031966 (mitochondrial membrane), GO:0005743 (mitochondrial inner membrane)