Protein: DNA replication licensing factor MCM2 (EC 3.6.4.12) (Minichromosome maintenance protein 2) (P29469)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity; specifically the MCM2-MCM5 association is proposed to be reversible and to mediate a open ring conformation which may facilitate DNA loading. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity. Necessary for cell growth.
Subcellular location: Nucleus.
Sequence: MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPDIDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERDRLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREELTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSESLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDFPTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIRISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKPGEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEEEREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVLLLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLADKGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNSTLPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELKNNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQMDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKVVVDSFVDAQKVSVRRQLRRSFAIYTLGH
InterPro domains: - IPR031327: Mini-chromosome maintenance protein (family) [204-851]
- IPR027925: MCM, N-terminal domain (domain) [209-298]
- IPR033762: MCM OB domain (domain) [306-432]
- IPR041562: MCM, AAA-lid domain (domain) [761-844]
- IPR008045: DNA replication licensing factor Mcm2 (family) [52-460]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [473-868]
- IPR018525: Mini-chromosome maintenance, conserved site (conserved_site) [602-610]
- IPR001208: MCM domain (domain) [481-703]
- IPR012340: Nucleic acid-binding, OB-fold (homologous_superfamily) [211-454]
Interaction partners: - Minichromosome maintenance protein 5
- Topoisomerase 1-associated factor 1
- DNA replication licensing factor MCM3
- Origin recognition complex subunit 3
- Origin recognition complex subunit 6
- DNA replication complex GINS protein PSF1
- Minichromosome maintenance protein 10
- DNA replication complex GINS protein PSF3
- DNA polymerase epsilon catalytic subunit A
- DDK kinase regulatory subunit DBF4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0004386 (helicase activity), GO:1901363 (heterocyclic compound binding), GO:0008094 (ATP-dependent activity, acting on DNA), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003678 (DNA helicase activity), GO:0140097 (catalytic activity, acting on DNA), GO:0043138 (3'-5' DNA helicase activity), GO:0017116 (single-stranded DNA helicase activity), GO:0003677 (DNA binding), GO:0003690 (double-stranded DNA binding), GO:0043565 (sequence-specific DNA binding), GO:0003697 (single-stranded DNA binding), GO:1990837 (sequence-specific double-stranded DNA binding), GO:0003688 (DNA replication origin binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:0051716 (cellular response to stimulus), GO:0071840 (cellular component organization or biogenesis), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0006259 (DNA metabolic process), GO:0006996 (organelle organization), GO:0006260 (DNA replication), GO:0006270 (DNA replication initiation), GO:0006310 (DNA recombination), GO:0006281 (DNA repair), GO:0051276 (chromosome organization), GO:0071103 (DNA conformation change), GO:0006261 (DNA-templated DNA replication), GO:0000725 (recombinational repair), GO:0006302 (double-strand break repair), GO:0000724 (double-strand break repair via homologous recombination), GO:0006268 (DNA unwinding involved in DNA replication), GO:0032392 (DNA geometric change), GO:0032508 (DNA duplex unwinding)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0032993 (protein-DNA complex), GO:0042555 (MCM complex), GO:0005657 (replication fork), GO:0098687 (chromosomal region), GO:0140513 (nuclear protein-containing complex), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0031261 (DNA replication preinitiation complex), GO:0043229 (intracellular organelle), GO:0031298 (replication fork protection complex), GO:0043596 (nuclear replication fork), GO:0043228 (non-membrane-bounded organelle), GO:0043233 (organelle lumen), GO:0005656 (nuclear pre-replicative complex), GO:0036387 (pre-replicative complex), GO:0043227 (membrane-bounded organelle), GO:0000781 (chromosome, telomeric region), GO:0043231 (intracellular membrane-bounded organelle), GO:0071162 (CMG complex), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0005634 (nucleus), GO:0031981 (nuclear lumen), GO:0005694 (chromosome), GO:0000228 (nuclear chromosome)