Protein: Galactofuranosyltransferase GlfT1 (GalTr 1) (EC 2.4.1.287) (Arabinogalactan galactosyltransferase 1) (Rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,4/1,5-galactofuranosyltransferase) (UDP-Galf:alpha-3-L-rhamnosyl-alpha-D-GlcNAc-pyrophosphate polyprenol, galactofuranosyl transferase) (P9WMX3)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Involved in the biosynthesis of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacterial cell wall. Catalyzes the transfer of the first two galactofuranosyl (Galf) units from UDP-galactofuranose (UDP-Galf) onto the rhamnosyl-GlcNAc-diphospho-decaprenol (Rha-GlcNAc-PP-C50) acceptor, yielding galactofuranosyl-galactofuranosyl-rhamnosyl-GlcNAc-diphospho-decaprenol (Galf-Galf-Rha-GlcNAc-PP-C50). Thus, GlfT1 is the initiator of galactan synthesis, while GlfT2 continues with the subsequent polymerization events.
Subcellular location: Cell membrane {ECO:0000269|PubMed:16952951}. Secreted, cell wall {ECO:0000269|PubMed:16952951}.
Sequence: MTESVFAVVVTHRRPDELAKSLDVLTAQTRLPDHLIVVDNDGCGDSPVRELVAGQPIATTYLGSRRNLGGAGGFALGMLHALAQGADWVWLADDDGHAQDARVLATLLACAEKYSLAEVSPMVCNIDDPTRLAFPLRRGLVWRRRASELRTEAGQELLPGIASLFNGALFRASTLAAIGVPDLRLFIRGDEVEMHRRLIRSGLPFGTCLDAAYLHPCGSDEFKPILCGRMHAQYPDDPGKRFFTYRNRGYVLSQPGLRKLLAQEWLRFGWFFLVTRRDPKGLWEWIRLRRLGRREKFGKPGGSA
InterPro domains: - IPR029044: Nucleotide-diphospho-sugar transferases (homologous_superfamily) [3-277]
- IPR001173: Glycosyltransferase 2-like (domain) [8-116]
Interaction partners: - Probable O-antigen/lipopolysaccharide transport integral membrane protein ABC transporter RfbD
- Galactofuranosyltransferase GlfT2
- Probable O-antigen/lipopolysaccharide transport ATP-binding protein ABC transporter RfbE
- N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase
- UDP-galactopyranose mutase
- Bacterial proteasome activator
- Probable conserved transmembrane protein alanine and leucine rich
- Decaprenyl-phosphate N-acetylglucosaminephosphotransferase
- Galactan 5-O-arabinofuranosyltransferase
- Conserved protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016757 (glycosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0071554 (cell wall organization or biogenesis), GO:0044238 (primary metabolic process), GO:0044249 (cellular biosynthetic process), GO:0016043 (cellular component organization), GO:0071669 (plant-type cell wall organization or biogenesis), GO:0005975 (carbohydrate metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044085 (cellular component biogenesis), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0042546 (cell wall biogenesis), GO:0009059 (macromolecule biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0045229 (external encapsulating structure organization), GO:0009832 (plant-type cell wall biogenesis), GO:0044264 (cellular polysaccharide metabolic process), GO:0016051 (carbohydrate biosynthetic process), GO:0005976 (polysaccharide metabolic process), GO:0034637 (cellular carbohydrate biosynthetic process), GO:0000271 (polysaccharide biosynthetic process), GO:0033692 (cellular polysaccharide biosynthetic process), GO:0044042 (glucan metabolic process), GO:0006073 (cellular glucan metabolic process), GO:0009250 (glucan biosynthetic process), GO:0051273 (beta-glucan metabolic process), GO:0051274 (beta-glucan biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)