Protein: Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 (EC 3.2.1.113) (EC 3.2.1.209) (Q93Y37)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Class I alpha-mannosidase essential for early N-glycan processing. Removes preferentially alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2). Involved in root development and cell wall biosynthesis.
Subcellular location: Golgi apparatus, cis-Golgi network membrane {ECO:0000305|PubMed:20023195}; Single-pass type II membrane protein {ECO:0000269|PubMed:20023195}.
Sequence: MSKSLPYSVKDIHYDNAKFRHRSPLKVFSQSLLTLSTKRNYASCSTGKFLILILFFGVACLMLMSKSPNESGLNEKGKVTFVGGLRLGGLLRKPPRLPPRLSPDEGQLRGSSTNGSTISNSDPKWAARQQSVKEAFDHAWSGYRKYAMGYDELMPISQKGVDGLGGLGATVVDALDTAMIMGLDNIVSEAGSWVETHLLERISQKGQVNLFETTIRVLGGLLSAYHLSGGEQGTVNMTHVGPKPVIYLNIAKDLADRLLSAFTSSPTPVPFCDVILHESTAHPAPGGASSTAEVASVQLEFNYLSSISGDPKYSTEAMKVLAHIKTLPKTEGLVPIYISPQTGDFVGENIRLGSRGDSYYEYLIKVWLQQGAKLNSNFTYLHDMYIEAMKGVRHLLVQNSIPKGLVFVGELPYGSKGEFSPKMDHLVCFLPGTLALGATKGLTKEQALKENLLSFEDLENLKLAEDLAKTCFEMYEVTATGLAPEIAYFHTKDYTEDGLDGGNKSSMYANDIIIKPADRHNLLRPETVESLFVLYRITKDTKYRDQGWQIFEAFEKYTKVKSGGYTSLDDVTEVPPHRRDKMETFFLGETLKYLYLLFGDDSVIPLDKFVFNTEAHPLPIRRNT
InterPro domains: - IPR001382: Glycoside hydrolase family 47 (family) [135-620]
- IPR012341: Six-hairpin glycosidase-like superfamily (homologous_superfamily) [125-624]
- IPR036026: Seven-hairpin glycosidases (homologous_superfamily) [124-622]
- IPR050749: Glycosyl Hydrolase Family 47 (family) [39-622]
Interaction partners: - Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1
- Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2
- Probable glucan 1,3-alpha-glucosidase
- Alpha-mannosidase 2
- Protein OS-9 homolog
- Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
- Dol-P-Man:Man
- Alpha-1,2-mannosyltransferase ALG9
- Calnexin homolog 1
- Dol-P-Man:Man
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016798 (hydrolase activity, acting on glycosyl bonds), GO:0004553 (hydrolase activity, hydrolyzing O-glycosyl compounds), GO:0015923 (mannosidase activity), GO:0004559 (alpha-mannosidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044238 (primary metabolic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0009100 (glycoprotein metabolic process), GO:0006491 (N-glycan processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031984 (organelle subcompartment), GO:0005794 (Golgi apparatus), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043227 (membrane-bounded organelle), GO:0098791 (Golgi apparatus subcompartment), GO:0031985 (Golgi cisterna), GO:0043231 (intracellular membrane-bounded organelle), GO:0005795 (Golgi stack), GO:0031982 (vesicle), GO:0005802 (trans-Golgi network), GO:0000138 (Golgi trans cisterna), GO:0097708 (intracellular vesicle)