Protein: Regulator of sigma-E protease RseP (P0AEH1)
Organism: Escherichia coli (strain K12)
Function: A site-2 regulated intramembrane protease (S2P) that cleaves the peptide bond between 'Ala-108' and 'Cys-109' in the transmembrane region of RseA. Part of a regulated intramembrane proteolysis (RIP) cascade. Acts on DegS-cleaved RseA to release the cytoplasmic domain of RseA, residue 'Val-148' of RseA may be required for this. This provides the cell with sigma-E (RpoE) activity through the proteolysis of RseA. Can also cleave sequences in transmembrane regions of other proteins (such as LacY) as well as liberated signal peptides of beta-lactamase, OmpF, LivK, SecM, PhoA, LivJ, OmpC, Lpp and TorA, probably within the membrane. Cleaves FecR within its transmembrane region to release an N- terminal cytoplasmic fragment which binds to sigma factor FecI, allowing it to activate transcription of the fecABCDE operon which mediates ferric citrate transport
Subcellular location: Cell inner membrane; Multi-pass membrane protein
Sequence: MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIAAGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETPDWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPRGPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEIERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKTWQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGIINLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
InterPro domains: - IPR001478: PDZ domain (domain) [115-291]
- IPR008915: Peptidase M50 (domain) [11-438]
- IPR004387: Peptidase M50, putative membrane-associated zinc metallopeptidase (family) [3-450]
- IPR041489: PDZ domain 6 (domain) [227-278]
- IPR036034: PDZ superfamily (homologous_superfamily) [86-310]
Interaction partners: - Outer membrane protein assembly factor BamA
- Anti-sigma-E factor RseA
- Serine endoprotease DegS
- Sigma-E factor regulatory protein RseB
- Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific)
- Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific)
- Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific)
- Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific)
- Phosphatidate cytidylyltransferase
- Periplasmic serine endoprotease DegP
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0008233 (peptidase activity), GO:0004175 (endopeptidase activity), GO:0008237 (metallopeptidase activity), GO:0004222 (metalloendopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0051716 (cellular response to stimulus), GO:0009628 (response to abiotic stimulus), GO:0006950 (response to stress), GO:0042221 (response to chemical), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0001666 (response to hypoxia), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0070482 (response to oxygen levels), GO:0043170 (macromolecule metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0071456 (cellular response to hypoxia), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0071453 (cellular response to oxygen levels), GO:0036293 (response to decreased oxygen levels), GO:0036294 (cellular response to decreased oxygen levels), GO:0070647 (protein modification by small protein conjugation or removal), GO:0032446 (protein modification by small protein conjugation), GO:0016567 (protein ubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)