Protein: Retinal-specific phospholipid-transporting ATPase ABCA4 (EC 7.6.2.1) (ATP-binding cassette sub-family A member 4) (RIM ABC transporter) (RIM protein) (RmP) (Retinal-specific ATP-binding cassette transporter) (F1MWM0)
Organism: Bos taurus (Bovine)
Function: Flippase that catalyzes in an ATP-dependent manner the transport of retinal-phosphatidylethanolamine conjugates like the 11-cis and all-trans isomers of N-retinylidene-phosphatidylethanolamine from the lumen to the cytoplasmic leaflet of photoreceptor outer segment disk membranes, where N-cis-retinylidene-phosphatidylethanolamine (N-cis-R-PE) is then isomerized to its all-trans isomer (N-trans-R-PE) and reduced by RDH8 to produce all-trans-retinol (all-trans-rol) and therefore prevents the accumulation of excess of 11-cis-retinal and its schiff-base conjugate and the formation of toxic bisretinoid. Displays both ATPase and GTPase activity that is strongly influenced by the lipid environment and the presence of retinoid compounds. Binds the unprotonated form of N-retinylidene-phosphatidylethanolamine with high affinity in the absence of ATP and ATP binding and hydrolysis induce a protein conformational change that causes the dissociation of N-retinylidene-phosphatidylethanolamine.
Subcellular location: Membrane {ECO:0000255}; Multi-pass membrane protein {ECO:0000255}. Cell projection, cilium, photoreceptor outer segment {ECO:0000269|PubMed:9092582, ECO:0000269|PubMed:9202155, ECO:0000269|PubMed:9288089}. Cytoplasmic vesicle {ECO:0000269|PubMed:21721517}. Endoplasmic reticulum {ECO:0000250|UniProtKB:P78363}. Note=Localized to the rim and incisures of rod outer segments disks. {ECO:0000269|PubMed:9092582, ECO:0000269|PubMed:9288089}.
Sequence: MGFARQIKLLLWKNWTLRKRQKIRFVVELVWPLSLFLVLIWLRNVNPLYSKHECHFPNKAMPSAGMLPWLQGIFCNVNNPCFQSPTAGESPGIVSNYNNSILARVYRDFQELLMDAPESQHLGQVWRELRTLSQLMNTLRMHPERIAGRGIRIREVLKDDEMLTLFLVKNIGLSDSVVYLLVNSQVRPEQFARGVPDLMLKDIACSEALLERFLIFPQRRAAQTVRGSLCSLSQGTLQWMEDTLYANVDFFKLFHVFPRLLDSRSQGMNLRSWGRILSDMSPRIQEFIHRPSVQDLLWVTRPLVQTGGPETFTQLMGILSDLLCGYPEGGGSRVFSFNWYEDNNYKAFLGIDSTRKDPIYSYDERTTTFCNALIQSLESNPLTKIAWRAAKPLLMGKILFTPDSPATRRILKNANSTFEELERVRKLVKVWEEVGPQIWYFFDKSTQMSMIRDTLENPTVKAFWNRQLGEEGITAEAVLNFLYNGPREGQADDVDNFNWRDIFNITDRALRLANQYLECLILDKFESYDDEFQLTQRALSLLEENRFWAGVVFPDMHPWTSSLPPHVKYKIRMDIDVVEKTNKIKDRYWDSGPRADPVEDFRYIWGGFAYLQDMVEHGITRSQAQEEVPVGIYLQQMPYPCFVDDSFMIILNRCFPIFMVLAWIYSVSMTVKSIVLEKELRLKETLKNQGVSNRVIWCTWFLDSFSIMSMSICLLTIFIMHGRILHYSNPFILFLFLLAFSIATIMQCFLLSTFFSRASLAAACSGVIYFTLYLPHILCFAWQDRITADMKMAVSLLSPVAFGFGTEYLARFEEQGVGLQWSNIGNSPMEGDEFSFLMSMKMMLLDAALYGLLAWYLDQVFPGDYGTPLPWYFLLQESYWLGGEGCSTREERALEKTEPITEEMEDPEYPEGINDCFFERELPGLVPGVCVKNLVKIFEPYGRPAVDRLNITFYESQITAFLGHNGAGKTTTLSIMTGLLPPTSGTVLVGGKDIETNLDAIRQSLGMCPQHNILFHHLTVAEHILFYAQLKGRSWDKAQLEMEAMLEDTGLHHKRNEEAQDLSGGVQRKLSVAIAFVGDAKVVVLDEPTSGVDPYSRRSIWDLLLKYRSGRTIIMSTHHMDEADILGDRIAIISQGRLYCSGTPLFLKNCFGTGFYLTLVRRMKTIQSQGRGREATCSCASKGFSVRCPACAEAITPEQVLDGDVNELTDMVHHHVPEAKLVECIGQELIFLLPNKNFKQRAYASLFRELEETLADLGLSSFGISDTPLEEIFLKVTEDLDSGHLFAGGTQQKRENINLRHPCSGPSEKAGQTPQGSSSHPREPAAHPEGQPPPEREGHSRLNSGARLIVQHVQALLVKRFQHTIRSHKDFLAQIVLPATFVFLALMLSLIIPPFGEYPALTLHPWMYGQQYTFFSMDQLDSEWLSALADVLVNKPGFGNRCLKEEWLPEFPCGNSSPWKTPSVSPDVTHLLQQQKWTADQPSPSCRCSTREKLTMLPECPEGAGGLPPPQRIQRSTEILQDLTDRNVSDFLVKTYPALIRSSLKSKFWVNEQRYGGISVGGKLPAPPFTGEALVGFLSDLGQLMNVSGGPMTREAAKEMPAFLKQLETEDNIKVWFNNKGWHALVSFLNVAHNAILRASLHKDKNPEEYGITVISQPLNLTKEQLSEITVLTTSVDAVVAICVIFAMSFVPASFVLYLIQERVNKAKHLQFVSGVSPTTYWLTNFLWDIMNYTVSAALVVGIFIGFQKKAYTSSENLPALVALLMLYGWAVIPMMYPASFLFDIPSTAYVALSCANLFIGINSSAITFVLELFENNRTLLRINAMLRKLLIIFPHFCLGRGLIDLALSQAVTDVYARFGEEHSSNPFQWDLIGKNLAAMAVEGVVYFLLTLLIQYQFFFSRWTTEPAKEPITDEDDDVAEERQRIISGGNKTDILRLNELTKVYSGTSSPAVDRLCVGVRPGECFGLLGVNGAGKTTTFKMLTGDTAVTSGDATVAGKSILTNISDVHQSMGYCPQFDAIDDLLTGREHLYLYARLRGVPAEEIERVTNWSIQSLGLSLYADRLAGTYSGGNKRKLSTAIALIGCPPLVLLDEPTTGMDPQARRMLWNTIMGIIREGRAVVLTSHSMEECEALCTRLAIMVKGAFQCLGTIQHLKSKFGDGYIVTMKIRSPKDDLLPDLGPVEQFFQGNFPGSVQRERHYNMLQFQVSSSSLARIFRLLVSHKDSLLIEEYSVTQTTLDQVFVNFAKQQNETYDLPLHPRAAGASRQAKEVDKGNSAPQG
InterPro domains: - IPR013525: ABC-2 type transporter, transmembrane domain (domain) [631-1893]
- IPR005951: Retinal-specific ATP-binding cassette transporter (family) [1-2270]
- IPR003593: AAA+ ATPase domain (domain) [955-2146]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [927-2172]
- IPR003439: ABC transporter-like, ATP-binding domain (domain) [929-2168]
- IPR056264: ABCA1-4-like, C-terminal R2 regulatory domain (domain) [2160-2236]
- IPR026082: ABC transporter A (family) [1915-2253]
Interaction partners: - Retinol dehydrogenase 8
- Peripherin-2
- Retinoid isomerohydrolase
- Rhodopsin
- Retinol dehydrogenase 12
- Short-chain dehydrogenase/reductase family 9C member 7
- X-linked retinitis pigmentosa GTPase regulator
- Unconventional myosin-VIIa
- Guanine nucleotide-binding protein G
- Centrosomal protein of 290 kDa
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005215 (transporter activity), GO:0140657 (ATP-dependent activity), GO:0140326 (ATPase-coupled intramembrane lipid transporter activity), GO:0005319 (lipid transporter activity), GO:0042626 (ATPase-coupled transmembrane transporter activity), GO:0022857 (transmembrane transporter activity), GO:0140303 (intramembrane lipid transporter activity), GO:0005548 (phospholipid transporter activity), GO:0140327 (flippase activity), GO:0022804 (active transmembrane transporter activity), GO:0015399 (primary active transmembrane transporter activity), GO:0140333 (glycerophospholipid flippase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0051234 (establishment of localization), GO:0006810 (transport), GO:0071702 (organic substance transport), GO:0071705 (nitrogen compound transport), GO:0015833 (peptide transport), GO:0042886 (amide transport), GO:0006857 (oligopeptide transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0001750 (photoreceptor outer segment), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0043227 (membrane-bounded organelle), GO:0120025 (plasma membrane bounded cell projection), GO:0005929 (cilium), GO:0043005 (neuron projection), GO:0097730 (non-motile cilium), GO:0097733 (photoreceptor cell cilium), GO:0097731 (9+0 non-motile cilium)