Protein: E3 ubiquitin-protein ligase RMD5 (EC 2.3.2.27) (GID complex catalytic subunit 2) (Glucose-induced degradation protein 2) (Required for meiotic nuclear division protein 5) (Sporulation protein RMD5) (Q12508)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: E3 ubiquitin-protein ligase component of the GID complex. Required for the adaptation to the presence of glucose in the growth medium; mediates the degradation of enzymes involved in gluconeogenesis when cells are shifted to glucose-containing medium. Required for proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBP1).
Subcellular location: Cytoplasm {ECO:0000269|PubMed:12686616}.
Sequence: MSELLDSFETEFAKFYTDSNLEETNLQKCLDHTHEFKSQLKKLKAHLNKHIQESKPEVYNKLSDKEKQKFKRKRELIIEKLSKSQRQWDHSVKKQIKYVSQQSNRFNKSTLNKLKEFDIDSVYVNKLPKETMENVNEAIGYHILRYSIDNMPLGNKNEAFQYLKDVYGITNKESTEFIEMGQIVHDLKKGDTESCLKWCSNEMESLSSNHTALSSLKFDLYTLSAMQIVKHGNPVELYYQITQNAPLDCFRHREKELMQNVVPLLTKSLIGQPIEDIDSKVNKELKECTSLFIKEYCAAKHIFFDSPLFLIVLSGLISFQFFIKYKTIRELAHVDWTTKDELPFDVKLPDFLTHFHPIFICPVLKEETTTENPPYSLACHHIISKKALDRLSKNGTITFKCPYCPVNTSMSSTKKVRFVML
InterPro domains: - IPR006595: CTLH, C-terminal LisH motif (domain) [176-236]
- IPR037683: Rmd5, degenerated RING (dRING) finger (domain) [359-406]
- IPR045098: Fyv10 family (family) [19-416]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [353-420]
- IPR024964: CTLH/CRA C-terminal to LisH motif domain (domain) [177-321]
- IPR044063: Gid-type RING finger domain (domain) [361-404]
Interaction partners: - GID complex subunit 8
- GID complex subunit 1
- GID complex subunit 9
- GID complex subunit 7
- GID complex subunit 5
- GID complex substrate-recognition subunit 4
- GID complex substrate-recognition subunit 10
- Protein yippee-like MOH1
- Ubiquitin-conjugating enzyme E2-24 kDa
- Fructose-1,6-bisphosphatase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0019787 (ubiquitin-like protein transferase activity), GO:0004842 (ubiquitin-protein transferase activity), GO:0061659 (ubiquitin-like protein ligase activity), GO:0061630 (ubiquitin protein ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044238 (primary metabolic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0006807 (nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0044248 (cellular catabolic process), GO:0009057 (macromolecule catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0044265 (cellular macromolecule catabolic process), GO:0030163 (protein catabolic process), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0010498 (proteasomal protein catabolic process), GO:0051603 (proteolysis involved in protein catabolic process), GO:0019941 (modification-dependent protein catabolic process), GO:0043161 (proteasome-mediated ubiquitin-dependent protein catabolic process), GO:0006511 (ubiquitin-dependent protein catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0140535 (intracellular protein-containing complex), GO:1990234 (transferase complex), GO:0000151 (ubiquitin ligase complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)