Protein: Creatine kinase B-type (Q04447)
Organism: Mus musculus (Mouse)
Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N- phosphocreatine as heat without performing any mechanical or chemical work
Subcellular location: Cytoplasm, cytosol. Mitochondrion. Cell membrane. Note=Localizes to the mitochondria of thermogenic fat cells via the internal MTS-like signal (iMTS-L) region
Sequence: MPFSNSHNTQKLRFPAEDEFPDLSSHNNHMAKVLTPELYAELRAKCTPSGFTLDDAIQTGVDNPGHPYIMTVGAVAGDEESYDVFKDLFDPIIEERHGGYQPSDEHKTDLNPDNLQGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCSRGERRAIEKLAVEALSSLDGDLSGRYYALKSMTEAEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKTFLVWINEEDHLRVISMQKGGNMKEVFTRFCTGLTQIETLFKSKNYEFMWNPHLGYILTCPSNLGTGLRAGVHIKLPHLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVFDVSNADRLGFSEVELVQMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK
InterPro domains: - IPR022414: ATP:guanido phosphotransferase, catalytic domain (domain) [125-367]
- IPR022413: ATP:guanido phosphotransferase, N-terminal (domain) [11-98]
- IPR000749: ATP:guanido phosphotransferase (family) [12-378]
- IPR022415: ATP:guanido phosphotransferase active site (active_site) [283-289]
- IPR036802: ATP:guanido phosphotransferase, N-terminal domain superfamily (homologous_superfamily) [1-107]
- IPR014746: Glutamine synthetase/guanido kinase, catalytic domain (homologous_superfamily) [105-380]
Interaction partners: - Creatine kinase M-type
- Ankyrin repeat and SOCS box protein 9
- Mitochondrial disaggregase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016775 (phosphotransferase activity, nitrogenous group as acceptor), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0006575 (cellular modified amino acid metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0019637 (organophosphate metabolic process), GO:0006793 (phosphorus metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0042398 (cellular modified amino acid biosynthetic process), GO:0090407 (organophosphate biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043209 (myelin sheath), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)