Protein: Probable lanosterol 14-alpha demethylase (Q1ZXH9)
Organism: Dictyostelium discoideum (Social amoeba)
Function: Sterol 14alpha-demethylase that plays a critical role in the third module of ergosterol biosynthesis pathway, being ergosterol the major sterol component in cell membranes that participates in a variety of functions. Starting from lanosterol (lanosta-8,24-dien-3beta-ol), it catalyzes the three-step oxidative removal of the 14alpha-methyl group (C-32) of the sterol in the form of formate, and converts the sterol to 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol, which is critical for ergosterol biosynthesis. Can demethylate substrates not intrinsic to yeast, such as eburicol (24-methylene-24,25-dihydrolanosterol) at a similar rate to lanosterol, and at a lower rate the 24,25- dihydrolanosterol (DHL) to 4,4-dimethyl-8,14-cholestadien-3beta-ol
Subcellular location: Membrane
Sequence: MIGTIAVLVIAILVIFAFKKSPSNIPPIVETIPFIGCFYQFAKNPLQLVRNSYDRLGEIFTLHLMGFKMTFVLGPEAQALFFRGTDEELSPKEAYRFVTPVFGKGVVYDSETEIMYEQLRFVKNGLVLSQLKKAVGIIQEETEKYFETKWGDSGEIDLLYEMNKLTILTASRCLMGKSINKSLGQSGQLADLYHELEEGLNPISFFFPNLPLPSFKKRDAARAKVAAIFHSIIQERRRSTDDSVDDVLYTLMNSKYKDGSVLEDEQIVGLMIGLLFAGQHTSSITLTYTIFYLLNNLEYFDETQKDINDIVQKENQGEINFDGLKRMNRLETVIREVLRLHPPLIFLMRKVMTPMEYKGKTIPAGHILAVSPQVGMRLPTVYKNPDSFEPKRFDVEDKTPFSFIAFGGGKHGCPGENFGILQIKTIWTVLSTKYNLEVGPVPPTDFTSLVAGPKGPCMVKYSKKQK
InterPro domains: - IPR050529: Cytochrome P450, sterol 14-alpha demethylase (family) [3-465]
- IPR017972: Cytochrome P450, conserved site (conserved_site) [406-415]
- IPR001128: Cytochrome P450 (family) [26-454]
- IPR036396: Cytochrome P450 superfamily (homologous_superfamily) [17-466]
- IPR002403: Cytochrome P450, E-class, group IV (family) [25-431]
Interaction partners: - Cycloartenol synthase
- Squalene synthase
- Delta(14)-sterol reductase
- Delta(14)-sterol reductase
- Protein erg2 homolog
- Squalene monooxygenase
- Cycloeucalenol cycloisomerase
- Putative methylsterol monooxygenase DDB_G0270946
- Sterol desaturase family protein
- Putative methylsterol monooxygenase DDB_G0269788
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0004497 (monooxygenase activity), GO:0016709 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0009058 (biosynthetic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0016125 (sterol metabolic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0008202 (steroid metabolic process), GO:0016126 (sterol biosynthetic process), GO:0006694 (steroid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)