Protein: Protein OrfX3 (A0A5P3XKL3)
Organism: Paraclostridium bifermentans (Clostridium bifermentans)
Function: Expression of the ptox operon (ntnh-orfX1-orfX2-orfX3-pmp1) in B.thuringiensis kills Anopheles but not Aedes mosquito 3rd instar larvae. The ntnh-pmp1 construct is about half as toxic
Sequence: MIGKRQTSTLNWDTVFAVPISVVNKAIKDKKSSPENFEFEDSSGSKCKGDFGDWQIITGGDGSNIRMKIPIYNFKAELVDDKYGIFNGNGGFESGEMNIQVKLKYFPHDKISKYKDVELVDLKVRSESADPIDPVVVMLSLKNLNGFYFNFLNEFGEDLQDIIEMFFIELVKQWLTENISLFNHIFSVVNLNLYIDQYSQWSWSRPSYVSYAYTDIEGDLDKSLLGVLCMTGGRNPDLRQQKVDPHAVPESSQCGFLIYEERVLRDLLLPTLPMKFKNSTVEDYEVINASGESGQYQYILRLKKGRSVSLDRVEANGSKYDPYMTEMSISLSNDVLKLEATTETSVGMGGKVGCDTINWYKLVLAKNGNGEQTISYEEVGEPTVINYVIKEGENWVWDVIAAIIAILATAVLAIFTGGAAFFIGGIVIAIITGFIAKTPDIILNWNLETSPSIDMMLENSTSQIIWNARDIFELDYVALNGPLQLGGELTV
InterPro domains: - IPR010567: Protein OrfX2/OrfX3/P47 (domain) [9-487]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0042802 (identical protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0048519 (negative regulation of biological process), GO:0016032 (viral process), GO:0044068 (modulation by symbiont of host cellular process), GO:0019222 (regulation of metabolic process), GO:0002682 (regulation of immune system process), GO:0051241 (negative regulation of multicellular organismal process), GO:0035821 (modulation of process of another organism), GO:0051239 (regulation of multicellular organismal process), GO:0044403 (biological process involved in symbiotic interaction), GO:0009892 (negative regulation of metabolic process), GO:0065008 (regulation of biological quality), GO:0002683 (negative regulation of immune system process), GO:0009607 (response to biotic stimulus), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0040013 (negative regulation of locomotion), GO:0035821 (modulation of process of another organism), GO:0019048 (modulation by virus of host process), GO:0048523 (negative regulation of cellular process), GO:0048585 (negative regulation of response to stimulus), GO:0040012 (regulation of locomotion), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0043207 (response to external biotic stimulus), GO:2000145 (regulation of cell motility), GO:0002685 (regulation of leukocyte migration), GO:0050920 (regulation of chemotaxis), GO:0080134 (regulation of response to stress), GO:0002686 (negative regulation of leukocyte migration), GO:0060255 (regulation of macromolecule metabolic process), GO:0032101 (regulation of response to external stimulus), GO:0001817 (regulation of cytokine production), GO:0031324 (negative regulation of cellular metabolic process), GO:0031348 (negative regulation of defense response), GO:0051701 (biological process involved in interaction with host), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0044003 (modulation by symbiont of host process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0001818 (negative regulation of cytokine production), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:2000146 (negative regulation of cell motility), GO:0032102 (negative regulation of response to external stimulus), GO:0031347 (regulation of defense response), GO:0052200 (response to host defenses), GO:0050728 (negative regulation of inflammatory response), GO:0051246 (regulation of protein metabolic process), GO:0051248 (negative regulation of protein metabolic process), GO:0032675 (regulation of interleukin-6 production), GO:0030334 (regulation of cell migration), GO:1902622 (regulation of neutrophil migration), GO:0010468 (regulation of gene expression), GO:0030336 (negative regulation of cell migration), GO:0034248 (regulation of amide metabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0032480 (negative regulation of type I interferon production), GO:0010563 (negative regulation of phosphorus metabolic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0002688 (regulation of leukocyte chemotaxis), GO:1903556 (negative regulation of tumor necrosis factor superfamily cytokine production), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0010629 (negative regulation of gene expression), GO:0051174 (regulation of phosphorus metabolic process), GO:0034249 (negative regulation of amide metabolic process), GO:0017148 (negative regulation of translation), GO:0031399 (regulation of protein modification process), GO:0031400 (negative regulation of protein modification process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0019220 (regulation of phosphate metabolic process), GO:0052572 (response to host immune response), GO:0030682 (mitigation of host defenses by symbiont), GO:0045936 (negative regulation of phosphate metabolic process), GO:0042325 (regulation of phosphorylation), GO:0001933 (negative regulation of protein phosphorylation), GO:0001932 (regulation of protein phosphorylation), GO:0042326 (negative regulation of phosphorylation), GO:0030833 (regulation of actin filament polymerization), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0070372 (regulation of ERK1 and ERK2 cascade)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0018995 (host cellular component), GO:0033643 (host cell part), GO:0043657 (host cell), GO:0033646 (host intracellular part), GO:0043656 (host intracellular region), GO:0033647 (host intracellular organelle), GO:0030430 (host cell cytoplasm), GO:0033648 (host intracellular membrane-bounded organelle), GO:0042025 (host cell nucleus)