Protein: 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase (HMP-P synthase) (Hydroxymethylpyrimidine phosphate synthase) (EC 2.-.-.-) (No message in thiamine protein 1) (Thiamine biosynthesis protein 3) (Thiamine pyrimidine synthase) (P36597)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Responsible for the formation of the pyrimidine heterocycle in the thiamine biosynthesis pathway. Catalyzes the formation of hydroxymethylpyrimidine phosphate (HMP-P) from histidine and pyridoxal phosphate (PLP). The protein uses PLP and the active site histidine to form HMP-P, generating an inactive enzyme. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus {ECO:0000269|PubMed:16823372}.
Sequence: MSTNKITFLTNWEATPYHLPIFLAQTRGYYEREGIEVAILEPTNPSDVTALIGSGKVDMGLKAMIHTLAAKARGYPVTSFGSLLNEPFTGLITLKGNGINDFKDIKGKRIGYVGEFGKIQLDDLCSKFGLSPSDYTAIRCGMNIAPAIINGEIDGGIGIECMQQVELERWCVSQGRPRSDVQMLRIDRLANLGCCCFCTILYIAHDEFIAKHPDKIKAFLRAIHSATLDMLKDPVQTYKEYIHFKREMGSELHREQFERCFAYFSHDISNVPRDWNKVTNYSKRLGIIPQDFEPNCTNGYLTWELDPDEKDPMGKQEAIAEIQDEIKQKGGVFSGNSLRYVEPANL
InterPro domains: - IPR015168: SsuA/THI5-like (domain) [16-236]
- IPR027939: NMT1/THI5 family (family) [1-307]
Interaction partners: - Thiamine thiazole synthase
- Probable thiamine biosynthetic bifunctional enzyme
- Putative hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 2
- Thiamine repressible genes regulatory protein thi1
- Putative hydroxymethylpyrimidine/phosphomethylpyrimidine kinase C18B5.05c
- Putative hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 1
- DNA polymerase epsilon catalytic subunit A
- Thiamine pyrophosphokinase
- Thiamine-repressible acid phosphatase pho4
- Thiamine repressible genes regulatory protein thi5
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0006066 (alcohol metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006766 (vitamin metabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006767 (water-soluble vitamin metabolic process), GO:0009110 (vitamin biosynthetic process), GO:0044272 (sulfur compound biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0034308 (primary alcohol metabolic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0042723 (thiamine-containing compound metabolic process), GO:0072527 (pyrimidine-containing compound metabolic process), GO:0046165 (alcohol biosynthetic process), GO:0042364 (water-soluble vitamin biosynthetic process), GO:0006772 (thiamine metabolic process), GO:0034309 (primary alcohol biosynthetic process), GO:0042724 (thiamine-containing compound biosynthetic process), GO:0072528 (pyrimidine-containing compound biosynthetic process), GO:0009228 (thiamine biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)