Protein: E3 ubiquitin-protein ligase ubr3 (EC 2.3.2.27) (E3 ubiquitin-protein transferase ubr3) (F1QJX5)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway (By similarity). Recognizes and binds to proteins bearing specific N-terminal residues, leading to their ubiquitination and subsequent degradation (By similarity). Positively regulates hedgehog/shh-signaling pathways that function in eye development, neuronal specification and somite development. Activation of shh up-regulates transcription of ubr3, which in turn promotes hedgehog/shh signaling possibly by controlling negative regulators such as Kif7.
Sequence: MMAASLLRRDKKSTAAHLKADLKRTDNSSGLRQLQELLDSVLNPERGSDPEALDWCKWLLAGGDGFDEFCRTVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAECFNNGDHTGHDFNMFRSQAGGACDCGDGNVMRESGFCNRHRLKTGENVPSVPRDLLLMSEMVLPRFIITIIQYLRDGYTEPESAADRDLQKVLQQLDPHISFLEELTKMGGAMRTVLTKILTDQQTFKELSMGQEDNVYAKKNYEKYLSALKSSGLVSVEEKGAAGGAGDGTSDAAAGAGALSLLGATATASLDDSSKEEDQDGLQGVGQRKRVKLSSSTKDPSIMDTLKHKCFLEELLFWTIKYEFPQKMVTFLLNMLPDQDYKITFTKTFVQHYAFIMKTLMKSHESDTMSNRIVHISVQLFSNEELARHVTEECQLLDIMVTVLLYMMESCLIKSELQDEENNRHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHSLLLLWMSFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLTHCKVKETQDYTKTVVRYCLETLQMWFDAIGFVDEPSLNQLTFHLPLHRYYAMFLSKGVKCQGLDLDSLLPDQEMLMKIMVHPLQIQASLSEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNAVLDSEQERPMLEGALTFLVILCSLRIHLGMSDDEILRAEMVSQLCMNDRTHSSLLDLIPENPNPKSGVVPGSCSFEEMLSGVADFKAPVFEPGGSMQQGMYTPKAEVWEKEFDPIMVILRTVYRRDVQSAMDRYSAFLKQSGVHTGNPWPPYKERTPLHPCYKGLVRLLHCKTLHIVIFTLLYKIWMDHQNMSEHVLCMVLYLIELGLDNQVQDDKVEEEPCIEEHCHDSWFPGTSLLSNLHHVINFVRVRVPETAPEVERKRERERERETPPSTSSESATFGQNLREAQVFSLVAERRRKFQEIINRSNHEASQAVRPKSSASRWLPPGTPPQLVTEILEIRESMLSLLVKLHQKLSAKQNSLSLSWLAEVDPAHHAHGDGLTAIERILAKASARSRHSKRCLQEICGKVCPPIPPKKISPGDKKSMDKEERRQRARERQQKLLAEFASRQKSFMETAMDVESPEADAVMDVSSEESLDSEVLYDCVICGQSGPSTEDRPTGLVVLLQASSVLGHRCRSDMPKRLPTTDEEHIYPEDTCGATHDVRLSLMQRYFKDSSCLQSVSIGWDGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGISVDKGEFTCPLCRQFANSVLPCRPGRGMETGAWHAPSTKSMSTLVKEVEDLQEQLGIFPVRASIKQRDPILIESNLSKEMESVIKDIKNTTQKKYMDYGKNPGSPDNDFLFMYSVARTNLELELVHRGGNLCSGGASAAAKRSCLNQLFHVLAMHMRLYSIDSAYNPWTRLTLSTQSRENEYCDEERPEVPMLFRDVPSLLIIFILTMPQPLRKEHFTCVVKVLYSLQFTQALAALSIRFSREERLAWSNTGAAKKNLPNSDKSWESLLGHMISELTKAKDVYDTNSEETSALSSSVWSPQSIEFRLQQFCLPFLRLSCLLQHHLYGDSLPGCLVEEEFSLLTRCLGLAASVQCSGSMSSAACLEWNISAFDLISQWCSEVVALSDTPSQQSASLLGQDPQWAAPRLLQLPDNYNTIFQYYHRKSCSSCGKTPKDPALCLVCGAFVCLKGHCCKQQGVCECVLHSQHCGAATGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYLCEERYRVLEQQWVAHTFDHINKRWGPHYNGL
InterPro domains: - IPR003126: Zinc finger, UBR-type (domain) [80-151]
- IPR039164: E3 ubiquitin-protein ligase UBR1-like (family) [10-1857]
- IPR044046: E3 ubiquitin-protein ligase UBR-like, C-terminal (domain) [1404-1845]
- IPR055194: E3 ubiquitin-protein ligase UBR1-like, winged-helix domain (domain) [749-849]
- IPR001841: Zinc finger, RING-type (domain) [1273-1328]
Interaction partners: - Ubiquitin protein ligase E3 component n-recognin 7.
- E3 ubiquitin-protein ligase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0019787 (ubiquitin-like protein transferase activity), GO:0004842 (ubiquitin-protein transferase activity), GO:0061659 (ubiquitin-like protein ligase activity), GO:0061630 (ubiquitin protein ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009605 (response to external stimulus), GO:0051707 (response to other organism), GO:0009607 (response to biotic stimulus), GO:0006950 (response to stress), GO:0006952 (defense response), GO:0009615 (response to virus), GO:0098542 (defense response to other organism), GO:0043207 (response to external biotic stimulus), GO:0140546 (defense response to symbiont), GO:0051607 (defense response to virus)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)