Protein: Protein pelota homolog (hPelota) (Protein Dom34 homolog) (Q9BRX2)
Organism: Homo sapiens (Human)
Function: Cotranslational quality control factor involved in the No-Go Decay (NGD) pathway. In the presence of ABCE1 and HBS1L, is required for 48S complex formation from 80S ribosomes and dissociation of vacant 80S ribosomes. Together with HBS1L and in presence of ABCE1, recognizes stalled ribosomes and promotes dissociation of elongation complexes assembled on non-stop mRNAs; this triggers endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and to degrade damaged mRNAs as part of the No-Go Decay (NGD) pathway. As part of the PINK1-regulated signaling, upon mitochondrial damage is recruited to the ribosome/mRNA-ribonucleoprotein complex associated to mitochondrial outer membrane thereby enabling the recruitment of autophagy receptors and induction of mitophagy.
Subcellular location: Cytoplasm {ECO:0000305|PubMed:21448132}.
Sequence: MKLVRKNIEKDNAGQVTLVPEEPEDMWHTYNLVQVGDSLRASTIRKVQTESSTGSVGSNRVRTTLTLCVEAIDFDSQACQLRVKGTNIQENEYVKMGAYHTIELEPNRQFTLAKKQWDSVVLERIEQACDPAWSADVAAVVMQEGLAHICLVTPSMTLTRAKVEVNIPRKRKGNCSQHDRALERFYEQVVQAIQRHIHFDVVKCILVASPGFVREQFCDYLFQQAVKTDNKLLLENRSKFLQVHASSGHKYSLKEALCDPTVASRLSDTKAAGEVKALDDFYKMLQHEPDRAFYGLKQVEKANEAMAIDTLLISDELFRHQDVATRSRYVRLVDSVKENAGTVRIFSSLHVSGEQLSQLTGVAAILRFPVPELSDQEGDSSSEED
InterPro domains: - IPR005142: eRF1 domain 3 (domain) [272-370]
- IPR029064: Ribosomal protein eL30-like superfamily (homologous_superfamily) [262-385]
- IPR005141: eRF1 domain 2 (domain) [136-268]
- IPR038069: Pelota/DOM34, N-terminal domain (homologous_superfamily) [1-132]
- IPR042226: eRF1, domain 2 superfamily (homologous_superfamily) [136-267]
- IPR005140: eRF1 domain 1/Pelota-like (domain) [1-130]
- IPR004405: Translation release factor pelota (family) [1-378]
Interaction partners: - HBS1-like protein
- Ubiquitin-like protein FUBI
- Small ribosomal subunit protein RACK1
- ATP-binding cassette sub-family E member 1
- Small ribosomal subunit protein uS3
- Small ribosomal subunit protein eS6
- Small ribosomal subunit protein uS10
- Small ribosomal subunit protein uS12
- Ubiquitin-ribosomal protein eS31 fusion protein
- Large ribosomal subunit protein uL3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140110 (transcription regulator activity), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:0003712 (transcription coregulator activity), GO:0005515 (protein binding), GO:0008134 (transcription factor binding), GO:0003676 (nucleic acid binding), GO:0140098 (catalytic activity, acting on RNA), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0003714 (transcription corepressor activity), GO:0140297 (DNA-binding transcription factor binding), GO:0004518 (nuclease activity), GO:0003723 (RNA binding), GO:0061629 (RNA polymerase II-specific DNA-binding transcription factor binding), GO:0003729 (mRNA binding), GO:0005881 (cytoplasmic microtubule), GO:0004519 (endonuclease activity), GO:0016922 (nuclear receptor binding), GO:0050681 (nuclear androgen receptor binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009058 (biosynthetic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0009892 (negative regulation of metabolic process), GO:0044237 (cellular metabolic process), GO:0048522 (positive regulation of cellular process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0044248 (cellular catabolic process), GO:0019538 (protein metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0016043 (cellular component organization), GO:0043603 (amide metabolic process), GO:0080090 (regulation of primary metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0034655 (nucleobase-containing compound catabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0006518 (peptide metabolic process), GO:0034250 (positive regulation of amide metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0006446 (regulation of translational initiation), GO:0019439 (aromatic compound catabolic process), GO:0046700 (heterocycle catabolic process), GO:0043604 (amide biosynthetic process), GO:0010628 (positive regulation of gene expression), GO:0045948 (positive regulation of translational initiation), GO:0010468 (regulation of gene expression), GO:1901566 (organonitrogen compound biosynthetic process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0009057 (macromolecule catabolic process), GO:0006412 (translation), GO:0034248 (regulation of amide metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0044265 (cellular macromolecule catabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:1901361 (organic cyclic compound catabolic process), GO:0051247 (positive regulation of protein metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0006996 (organelle organization), GO:0022411 (cellular component disassembly), GO:0010467 (gene expression), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0010629 (negative regulation of gene expression), GO:0016070 (RNA metabolic process), GO:0043043 (peptide biosynthetic process), GO:0006402 (mRNA catabolic process), GO:0006401 (RNA catabolic process), GO:1903008 (organelle disassembly), GO:0006414 (translational elongation), GO:2000112 (regulation of cellular macromolecule biosynthetic process), GO:0006417 (regulation of translation), GO:0045727 (positive regulation of translation), GO:0010608 (post-transcriptional regulation of gene expression), GO:0000956 (nuclear-transcribed mRNA catabolic process), GO:0016071 (mRNA metabolic process), GO:0034661 (ncRNA catabolic process), GO:0034660 (ncRNA metabolic process), GO:0016075 (rRNA catabolic process), GO:0016072 (rRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0016020 (membrane), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)