Protein: Probable WRKY transcription factor 54 (WRKY DNA-binding protein 54) (Q93WU8)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Together with WRKY70, negative regulator of developmental senescence, probably via the regulation of several senescence-associated markers genes. Positive regulator of EDS1-dependent defense against E.amylovora. In collaboration with WRKY70, prevents stomatal closure and, consequently, osmotic stress tolerance. Together with WRKY46 and WRKY70, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression. Negative regulator of SA biosynthesis. Prevents defense response to the necrotrophic pathogens P.carotovorum and B.cinerea, but promotes defense against biotrophic/hemibiotrophic pathogens P.syringae pv. tomato (Pst) DC3000, probably by regulating negatively the jasmonic acid (JA)/ethylene (ET) and positively the salicylic acid (SA) signaling pathways.
Subcellular location: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00223}.
Sequence: MDSNSNNTKSIKRKVVDQLVEGYEFATQLQLLLSHQHSNQYHIDETRLVSGSGSVSGGPDPVDELMSKILGSFHKTISVLDSFDPVAVSVPIAVEGSWNASCGDDSATPVSCNGGDSGESKKKRLGVGKGKRGCYTRKTRSHTRIVEAKSSEDRYAWRKYGQKEILNTTFPRSYFRCTHKPTQGCKATKQVQKQDQDSEMFQITYIGYHTCTANDQTHAKTEPFDQEIIMDSEKTLAASTAQNHVNAMVQEQENNTSSVTAIDAGMVKEEQNNNGDQSKDYYEGSSTGEDLSLVWQETMMFDDHQNHYYCGETSTTSHQFGFIDNDDQFSSFFDSYCADYERTSAM
InterPro domains: - IPR044810: WRKY transcription factor, plant (family) [18-230]
- IPR003657: WRKY domain (domain) [146-214]
- IPR036576: WRKY domain superfamily (homologous_superfamily) [133-212]
Interaction partners: - Probable WRKY transcription factor 51
- High mobility group B protein 6
- Isochorismate synthase 1, chloroplastic
- Probable WRKY transcription factor 70
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:1901363 (heterocyclic compound binding), GO:0003700 (DNA-binding transcription factor activity), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0001067 (transcription regulatory region nucleic acid binding), GO:0003677 (DNA binding), GO:0003690 (double-stranded DNA binding), GO:0043565 (sequence-specific DNA binding), GO:0000976 (transcription cis-regulatory region binding), GO:1990837 (sequence-specific double-stranded DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0008152 (metabolic process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0022414 (reproductive process), GO:0002376 (immune system process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0048856 (anatomical structure development), GO:0042221 (response to chemical), GO:0006955 (immune response), GO:0002684 (positive regulation of immune system process), GO:0006807 (nitrogen compound metabolic process), GO:0019748 (secondary metabolic process), GO:0090693 (plant organ senescence), GO:0003006 (developmental process involved in reproduction), GO:0009791 (post-embryonic development), GO:0009892 (negative regulation of metabolic process), GO:0050793 (regulation of developmental process), GO:0009607 (response to biotic stimulus), GO:0009719 (response to endogenous stimulus), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0002253 (activation of immune response), GO:0051093 (negative regulation of developmental process), GO:0048523 (negative regulation of cellular process), GO:0051707 (response to other organism), GO:0007165 (signal transduction), GO:0009628 (response to abiotic stimulus), GO:0023056 (positive regulation of signaling), GO:0007275 (multicellular organism development), GO:0009058 (biosynthetic process), GO:0048584 (positive regulation of response to stimulus), GO:0019222 (regulation of metabolic process), GO:0002682 (regulation of immune system process), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0002252 (immune effector process), GO:0006950 (response to stress), GO:0007154 (cell communication), GO:0048522 (positive regulation of cellular process), GO:0001666 (response to hypoxia), GO:0032103 (positive regulation of response to external stimulus), GO:1900055 (regulation of leaf senescence), GO:0043207 (response to external biotic stimulus), GO:0010150 (leaf senescence), GO:0080134 (regulation of response to stress), GO:0048731 (system development), GO:0070482 (response to oxygen levels), GO:0009966 (regulation of signal transduction), GO:0071495 (cellular response to endogenous stimulus), GO:0060255 (regulation of macromolecule metabolic process), GO:0032101 (regulation of response to external stimulus), GO:0048608 (reproductive structure development), GO:0009404 (toxin metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0009725 (response to hormone), GO:1901135 (carbohydrate derivative metabolic process), GO:0002218 (activation of innate immune response), GO:0019757 (glycosinolate metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0009755 (hormone-mediated signaling pathway), GO:0006082 (organic acid metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0050778 (positive regulation of immune response), GO:0031349 (positive regulation of defense response), GO:0009625 (response to insect), GO:0009620 (response to fungus), GO:0009967 (positive regulation of signal transduction), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006979 (response to oxidative stress), GO:0046483 (heterocycle metabolic process), GO:0006952 (defense response), GO:0006970 (response to osmotic stress), GO:0044249 (cellular biosynthetic process), GO:1901700 (response to oxygen-containing compound), GO:0034641 (cellular nitrogen compound metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0010033 (response to organic substance), GO:0045087 (innate immune response), GO:0098542 (defense response to other organism), GO:0044550 (secondary metabolite biosynthetic process), GO:0099402 (plant organ development), GO:0002831 (regulation of response to biotic stimulus), GO:0009863 (salicylic acid mediated signaling pathway), GO:0050776 (regulation of immune response), GO:0010646 (regulation of cell communication), GO:0070887 (cellular response to chemical stimulus), GO:0010119 (regulation of stomatal movement), GO:0033554 (cellular response to stress), GO:0002833 (positive regulation of response to biotic stimulus), GO:0090567 (reproductive shoot system development), GO:0009617 (response to bacterium), GO:0009889 (regulation of biosynthetic process), GO:0010647 (positive regulation of cell communication), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0048437 (floral organ development), GO:0080090 (regulation of primary metabolic process), GO:0006790 (sulfur compound metabolic process), GO:0002239 (response to oomycetes), GO:0031347 (regulation of defense response), GO:1901362 (organic cyclic compound biosynthetic process), GO:0045089 (positive regulation of innate immune response), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0071453 (cellular response to oxygen levels), GO:0043436 (oxoacid metabolic process), GO:2000022 (regulation of jasmonic acid mediated signaling pathway), GO:0070542 (response to fatty acid), GO:0010468 (regulation of gene expression), GO:0009753 (response to jasmonic acid), GO:0019760 (glucosinolate metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0016143 (S-glycoside metabolic process), GO:0009403 (toxin biosynthetic process), GO:2000070 (regulation of response to water deprivation), GO:0050832 (defense response to fungus), GO:0018130 (heterocycle biosynthetic process), GO:0036293 (response to decreased oxygen levels), GO:0009867 (jasmonic acid mediated signaling pathway), GO:0048827 (phyllome development), GO:1901657 (glycosyl compound metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0019758 (glycosinolate biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:1901701 (cellular response to oxygen-containing compound), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0009908 (flower development), GO:2000031 (regulation of salicylic acid mediated signaling pathway), GO:0090333 (regulation of stomatal closure), GO:0071456 (cellular response to hypoxia), GO:0061458 (reproductive system development), GO:0047484 (regulation of response to osmotic stress), GO:0045088 (regulation of innate immune response), GO:0009682 (induced systemic resistance), GO:0071310 (cellular response to organic substance), GO:0019438 (aromatic compound biosynthetic process), GO:0009751 (response to salicylic acid), GO:0042430 (indole-containing compound metabolic process), GO:0009627 (systemic acquired resistance), GO:1900457 (regulation of brassinosteroid mediated signaling pathway), GO:0052314 (phytoalexin metabolic process), GO:0048367 (shoot system development), GO:0002229 (defense response to oomycetes), GO:0032870 (cellular response to hormone stimulus), GO:0044272 (sulfur compound biosynthetic process), GO:0033993 (response to lipid), GO:0042742 (defense response to bacterium), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0014070 (response to organic cyclic compound), GO:0051253 (negative regulation of RNA metabolic process), GO:0000302 (response to reactive oxygen species), GO:1902531 (regulation of intracellular signal transduction), GO:1901659 (glycosyl compound biosynthetic process), GO:0070297 (regulation of phosphorelay signal transduction system), GO:0071395 (cellular response to jasmonic acid stimulus), GO:2001141 (regulation of RNA biosynthetic process), GO:0010104 (regulation of ethylene-activated signaling pathway), GO:0042435 (indole-containing compound biosynthetic process), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0048366 (leaf development), GO:0019761 (glucosinolate biosynthetic process), GO:0016144 (S-glycoside biosynthetic process), GO:0071398 (cellular response to fatty acid), GO:0006355 (regulation of DNA-templated transcription), GO:0071407 (cellular response to organic cyclic compound), GO:0071396 (cellular response to lipid), GO:0036294 (cellular response to decreased oxygen levels), GO:0052315 (phytoalexin biosynthetic process), GO:0071446 (cellular response to salicylic acid stimulus), GO:0045892 (negative regulation of DNA-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:1903507 (negative regulation of nucleic acid-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)