Protein: Cytosolic Fe-S cluster assembly factor NBP35 (Nucleotide binding protein 35) (AtNBP35) (Q8H1Q2)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development.
Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03038, ECO:0000269|PubMed:18957412, ECO:0000269|PubMed:19084504}.
Sequence: MENGDIPEDANEHCPGPQSESAGKSDSCAGCPNQEACATAPKGPDPDLVAIAERMSTVKHKILVLSGKGGVGKSTFSAQLSFALAGMDHQVGLMDIDICGPSIPKMLGLEGQEIHQSNLGWSPVYVEDNLGVMSIGFMLPNSDEAVIWRGPRKNGLIKQFLKDVYWGEIDYLVVDAPPGTSDEHISIVQYLLPTGIDGAIIVTTPQEVSLIDVRKEVSFCKKVGVPVLGVVENMSGLSQPLKDVKFMKLATETGSSINVTEDVIACLRKNAPELLDIVACSEVFDSSGGGAERMCREMGVPFLGKVPMDPQLCKAAEQGKSCFEDNKCLISAPALKSIIQKVVPSTVMTE
InterPro domains: - IPR028601: Cytosolic Fe-S cluster assembly factor NUBP1/Nbp35 (family) [7-347]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [45-346]
- IPR033756: Flagellum site-determining protein YlxH/ Fe-S cluster assembling factor NBP35 (family) [58-342]
- IPR019591: Mrp/NBP35 ATP-binding protein (family) [14-345]
- IPR000808: Iron-sulfur cluster carrier protein-like, conserved site (conserved_site) [166-182]
Interaction partners: - Protein NAR1
- MMS19 nucleotide excision repair protein homolog
- Protein AE7
- Iron-sulfur cluster assembly protein 1
- Cysteine desulfurase, mitochondrial
- Protein CHLOROPLAST IMPORT APPARATUS 2
- Protein CIA1
- Anamorsin homolog
- ABC transporter B family member 25, mitochondrial
- NADPH-dependent diflavin oxidoreductase 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0006790 (sulfur compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0022607 (cellular component assembly), GO:0043412 (macromolecule modification), GO:0016226 (iron-sulfur cluster assembly), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0031163 (metallo-sulfur cluster assembly), GO:0006396 (RNA processing), GO:0009451 (RNA modification), GO:0006400 (tRNA modification), GO:0034470 (ncRNA processing), GO:0034660 (ncRNA metabolic process), GO:0002097 (tRNA wobble base modification), GO:0006399 (tRNA metabolic process), GO:0008033 (tRNA processing), GO:0002098 (tRNA wobble uridine modification)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)