Protein: FAD-linked oxidoreductase ZEB1 (A0A0E0RTV6)
Organism: Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084
Function: FAD-linked oxidoreductase; part of the gene cluster that mediates the biosynthesis of zearalenone (ZEA), a nonsteroid estrogen that is a contaminant of cereal grains and causes estrogenic disorders in humans and animals. The ZEA backbone is synthesized from a single acetyl- CoA molecule and eight malonyl-CoA molecules. The reducing polyketide synthase ZEA2 is proposed to synthesize a reduced hexaketide intermediate by using different combinations of its reductive domains during each round of condensation. The hexaketide thioester is then transacylated to the non-reducing polyketide synthase ZEA1 and is further condensed with three malonyl-CoAs without reductive tailoring to yield a mixed reduced/unreduced nonaketide. ZEA1 must be able to interact with ZEA2 to facilitate starter-unit acyltransfer and initiate polyketide biosynthesis. ZEA1 also mediates the required C2-C7 cyclization to form the resorcylate core and catalyzes the formation of the macrolactone. ZEB1 is then responsible for the chemical conversion of beta-zearalenonol (beta-ZOL) to ZEA in the biosynthetic pathway
Sequence: MKLSPSKYLPVLLGTLSLTIANPSADCKCFPGDDCWPSAAEWKALNTSVSGNLIKTVPLGAPCHDPMFKGDVCQSLRQQWQNATIHFASSSSVMAPFFANQSCDPFQPRIRPCELGNYVSYAIAAETTSDVQNAIAFARANHIRLVIRNTGHDYLGRSTGAGALGVWTHHLKNIEFVDWDDDTYTGNAVKLGAGVQGFEVLEAARSRGLVVVGGECPTVGIAGGYSQGGGHSALSTSFGLSVDNVLSWEVITAKGELLTVNKDENPDLFWALRGGGGGTFGVVISMTVKAHPGTITSGASLSFSTDTNSEEAFWAGIQVFQDTLEDMVDAGTMVIHIITNTSFLIAPLTAYNKTEAQVKVIMKPFISSLTSKHVDFDVTYKESKTYYDHYNEFLGPLPYGKIRVGFEQYGGRLIPRSVVPNFTETLRQVTNMGVTWVGVATDVGPFGTRATTSVHPAWRSTLVHALLSTPWDFTKPWHDMIKLQDLMTNVIMPKVEAVTPGSGAYVNEADFRQPNYQDVFWGDNYKDLLEVKEKWDPEHFFFVPKGVGSEIWSIAEDGRMCKSAL
InterPro domains: - IPR016166: FAD-binding domain, PCMH-type (domain) [115-293]
- IPR006094: FAD linked oxidase, N-terminal (domain) [121-260]
- IPR050432: FAD-linked Oxidoreductases in Biosynthesis Pathways (family) [69-539]
- IPR036318: FAD-binding, type PCMH-like superfamily (homologous_superfamily) [63-292]
- IPR016169: FAD-binding, type PCMH, subdomain 2 (homologous_superfamily) [100-549]
- IPR012951: Berberine/berberine-like (domain) [504-547]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016614 (oxidoreductase activity, acting on CH-OH group of donors)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0016999 (antibiotic metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:0017000 (antibiotic biosynthetic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0044249 (cellular biosynthetic process), GO:0009404 (toxin metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0033067 (macrolide metabolic process), GO:0030638 (polyketide metabolic process), GO:1901334 (lactone metabolic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0043385 (mycotoxin metabolic process), GO:0009403 (toxin biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0033068 (macrolide biosynthetic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:0030639 (polyketide biosynthetic process), GO:0018958 (phenol-containing compound metabolic process), GO:0043386 (mycotoxin biosynthetic process), GO:1901336 (lactone biosynthetic process), GO:0046189 (phenol-containing compound biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005576 (extracellular region)