Protein: Protein mono-ADP-ribosyltransferase PARP3 (EC 2.4.2.-) (ADP-ribosyltransferase diphtheria toxin-like 3) (ARTD3) (DNA ADP-ribosyltransferase PARP3) (EC 2.4.2.-) (NAD(+) ADP-ribosyltransferase 3) (ADPRT-3) (Poly [ADP-ribose] polymerase 3) (PARP-3) (Poly[ADP-ribose] synthase 3) (pADPRT-3) (Q3ULW8)
Organism: Mus musculus (Mouse)
Function: Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage. Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins (By similarity). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (By similarity). Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as histone H2B, XRCC5 and XRCC6 (By similarity). ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (By similarity). Involved in single-strand break repair by catalyzing mono-ADP-ribosylation of histone H2B on 'Glu-2' (H2BE2ADPr) of nucleosomes containing nicked DNA (By similarity). Cooperates with the XRCC5-XRCC6 (Ku80-Ku70) heterodimer to limit end-resection thereby promoting accurate NHEJ (By similarity). Suppresses G-quadruplex (G4) structures in response to DNA damage (By similarity). Associates with a number of DNA repair factors and is involved in the response to exogenous and endogenous DNA strand breaks. Together with APLF, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ) (By similarity). May link the DNA damage surveillance network to the mitotic fidelity checkpoint (By similarity). Acts as a negative regulator of immunoglobulin class switch recombination, probably by controlling the level of AICDA /AID on the chromatin. In addition to proteins, also able to ADP-ribosylate DNA: mediates DNA mono-ADP-ribosylation of DNA strand break termini via covalent addition of a single ADP-ribose moiety to a 5'- or 3'-terminal phosphate residues in DNA containing multiple strand breaks (By similarity).
Subcellular location: Nucleus {ECO:0000250|UniProtKB:Q9Y6F1}. Chromosome {ECO:0000250|UniProtKB:Q9Y6F1}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:Q9Y6F1}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:Q9Y6F1}. Note=Almost exclusively localized in the nucleus and appears in numerous small foci and a small number of larger foci whereas a centrosomal location has not been detected. In response to DNA damage, localizes to sites of double-strand break. Also localizes to single-strand breaks. Preferentially localized to the daughter centriole. {ECO:0000250|UniProtKB:Q9Y6F1}.
Sequence: MAPKRKASVQTEGSKKRRQGTEEEDSFRSTAEALRAAPADNRVIRVDPSCPFSRNPGIQVHEDYDCTLNQTNIGNNNNKFYIIQLLEEGSRFFCWNRWGRVGEVGQSKMNHFTCLEDAKKDFKKKFWEKTKNKWEERDRFVAQPNKYTLIEVQGEAESQEAVVKALSPQVYSGPVRTVVKPCSLDPATQNLITNIFSKEMFKNAMTLMNLDVKKMPLGKLTKQQIARGFEALEALEEAMKNPTGDGQSLEELSSCFYTVIPHNFGRSRPPPINSPDVLQAKKDMLLVLADIELAQTLQAAPGEEEEKVEEVPHPLDRDYQLLRCQLQLLDSGESEYKAIQTYLKQTGNSYRCPDLRHVWKVNREGEGDRFQAHSKLGNRRLLWHGTNVAVVAAILTSGLRIMPHSGGRVGKGIYFASENSKSAGYVTTMHCGGHQVGYMFLGEVALGKEHHITIDDPSLKSPPSGFDSVIARGQTEPDPAQDIELELDGQPVVVPQGPPVQCPSFKSSSFSQSEYLIYKESQCRLRYLLEIHL
InterPro domains: - IPR008893: WGR domain (domain) [57-147]
- IPR004102: Poly(ADP-ribose) polymerase, regulatory domain (domain) [181-319]
- IPR050800: ADP-ribosyltransferase diphtheria toxin-like (family) [41-530]
- IPR036930: WGR domain superfamily (homologous_superfamily) [44-151]
- IPR036616: Poly(ADP-ribose) polymerase, regulatory domain superfamily (homologous_superfamily) [176-321]
- IPR012317: Poly(ADP-ribose) polymerase, catalytic domain (domain) [313-533]
Interaction partners: - Caspase-3
- Caspase-7
- Caspase-9
- Aprataxin and PNK-like factor
- Granzyme B
- X-ray repair cross-complementing protein 6
- Poly
- Histone PARylation factor 1
- Protein mono-ADP-ribosyltransferase PARP16
- Protein mono-ADP-ribosyltransferase PARP10
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016757 (glycosyltransferase activity), GO:0016763 (pentosyltransferase activity), GO:0003950 (NAD+ ADP-ribosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0065008 (regulation of biological quality), GO:0009628 (response to abiotic stimulus), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0008219 (cell death), GO:0006807 (nitrogen compound metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0001666 (response to hypoxia), GO:0012501 (programmed cell death), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0006979 (response to oxidative stress), GO:0046483 (heterocycle metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0070482 (response to oxygen levels), GO:0019538 (protein metabolic process), GO:1901700 (response to oxygen-containing compound), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0031647 (regulation of protein stability), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0034599 (cellular response to oxidative stress), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0062197 (cellular response to chemical stress), GO:0036293 (response to decreased oxygen levels), GO:0006915 (apoptotic process), GO:0006259 (DNA metabolic process), GO:0050821 (protein stabilization), GO:0006304 (DNA modification), GO:0006281 (DNA repair), GO:0016570 (histone modification), GO:0043687 (post-translational protein modification), GO:0006302 (double-strand break repair)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus)