Protein: Acyl-CoA dehydrogenase FadE12 (EC 1.3.99.-) (Medium chain acyl-CoA dehydrogenase) (MCADH) (P9WQG3)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Has acyl-CoA dehydrogenase activity on a number of substrates. N-octanoyl-CoA is the best tested, but n-lauroyl-CoA, n-palmitoyl-CoA and isovaleryl-CoA were also used.
Sequence: MTDTSFIESEERQALRKAVASWVANYGHEYYLDKARKHEHTSELWAEAGKLGFLGVNLPEEYGGGGAGMYELSLVMEEMAAAGSALLLMVVSPAINGTIIAKFGTDDQKKRWLPGIADGSLTMAFAITEPDAGSNSHKITTTARRDGSDWIIKGQKVFISGIDQAQAVLVVGRSEEAKTGKLRPALFVVPTDAPGFSYTPIEMELVSPERQFQVFLDDVRLPADALVGAEDAAIAQLFAGLNPERIMGAASAVGMGRFALGRAVDYVKTRKVWSTPIGAHQGLAHPLAQCHIEVELAKLMTQKAATLYDHGDDFGAAEAANMAKYAAAEASSRAVDQAVQSMGGNGLTKEYGVAAMMTSARLARIAPISREMVLNFVAQTSLGLPRSY
InterPro domains: - IPR037069: Acyl-CoA dehydrogenase/oxidase, N-terminal domain superfamily (homologous_superfamily) [2-120]
- IPR013786: Acyl-CoA dehydrogenase/oxidase, N-terminal (domain) [9-119]
- IPR036250: Acyl-CoA dehydrogenase-like, C-terminal (homologous_superfamily) [231-386]
- IPR006091: Acyl-CoA oxidase/dehydrogenase, middle domain (domain) [124-204]
- IPR050741: Acyl-CoA dehydrogenase (family) [9-382]
- IPR009100: Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain superfamily (homologous_superfamily) [5-244]
- IPR046373: Acyl-CoA oxidase/dehydrogenase, middle domain superfamily (homologous_superfamily) [121-229]
- IPR009075: Acyl-CoA dehydrogenase/oxidase, C-terminal (domain) [239-380]
Interaction partners: - Biotin-dependent 3-methylcrotonyl-coenzyme A carboxylase alpha1 subunit
- Probable biotin-dependent acyl-coenzyme A carboxylase beta2 subunit
- Probable acyl-CoA dehydrogenase FadE13
- Probable enoyl-CoA hydratase EchA7
- Exopolyphosphatase
- Probable fatty oxidation protein FadB
- Electron transfer flavoprotein subunit beta
- Electron transfer flavoprotein subunit alpha
- Enoyl-CoA hydratase EchA19
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0016491 (oxidoreductase activity), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:1901265 (nucleoside phosphate binding), GO:0016627 (oxidoreductase activity, acting on the CH-CH group of donors), GO:0052890 (oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor), GO:0050660 (flavin adenine dinucleotide binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0120252 (hydrocarbon metabolic process), GO:0006629 (lipid metabolic process), GO:1901575 (organic substance catabolic process), GO:0044248 (cellular catabolic process), GO:0006720 (isoprenoid metabolic process), GO:0042214 (terpene metabolic process), GO:0044242 (cellular lipid catabolic process), GO:0016042 (lipid catabolic process), GO:0120253 (hydrocarbon catabolic process), GO:0046247 (terpene catabolic process), GO:0008300 (isoprenoid catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0005886 (plasma membrane), GO:0009274 (peptidoglycan-based cell wall)