Protein: Ribosome assembly protein 4 (Notchless protein homolog 1) (Ribosome biogenesis factor RSA4) (P25382)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Involved in ribosome biogenesis. Required for processing and efficient intra-nuclear transport of pre-60S ribosomal subunits. Interacts with the AAA-ATPase Midasin (MDN1/REA1), which is essential for the ATP-dependent dissociation of a group of nonribosomal factors from the pre-60S particle.
Subcellular location: Nucleus, nucleolus {ECO:0000269|PubMed:16221974}.
Sequence: MSTLIPPPSKKQKKEAQLPREVAIIPKDLPNVSIKFQALDTGDNVGGALRVPGAISEKQLEELLNQLNGTSDDPVPYTFSCTIQGKKASDPVKTIDITDNLYSSLIKPGYNSTEDQITLLYTPRAVFKVKPVTRSSSAIAGHGSTILCSAFAPHTSSRMVTGAGDNTARIWDCDTQTPMHTLKGHYNWVLCVSWSPDGEVIATGSMDNTIRLWDPKSGQCLGDALRGHSKWITSLSWEPIHLVKPGSKPRLASSSKDGTIKIWDTVSRVCQYTMSGHTNSVSCVKWGGQGLLYSGSHDRTVRVWDINSQGRCINILKSHAHWVNHLSLSTDYALRIGAFDHTGKKPSTPEEAQKKALENYEKICKKNGNSEEMMVTASDDYTMFLWNPLKSTKPIARMTGHQKLVNHVAFSPDGRYIVSASFDNSIKLWDGRDGKFISTFRGHVASVYQVAWSSDCRLLVSCSKDTTLKVWDVRTRKLSVDLPGHKDEVYTVDWSVDGKRVCSGGKDKMVRLWTH
InterPro domains: - IPR001680: WD40 repeat (repeat) [132-515]
- IPR019775: WD40 repeat, conserved site (conserved_site) [292-473]
- IPR020472: PAC1/LIS1-like, WD-40 repeat (repeat) [251-473]
- IPR015943: WD40/YVTN repeat-like-containing domain superfamily (homologous_superfamily) [136-515]
- IPR012972: NLE (domain) [32-80]
- IPR036322: WD40-repeat-containing domain superfamily (homologous_superfamily) [113-513]
Interaction partners: - Nuclear GTP-binding protein NUG1
- Nucleolar GTP-binding protein 2
- Probable metalloprotease ARX1
- Ribosome biogenesis protein NSA2
- Ribosome biogenesis protein NOP53
- Midasin
- Pre-rRNA-processing protein IPI3
- Nucleolar GTP-binding protein 1
- Protein SDA1
- Pre-rRNA-processing protein RIX1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0043933 (protein-containing complex organization), GO:0010467 (gene expression), GO:0022607 (cellular component assembly), GO:0016070 (RNA metabolic process), GO:0022618 (ribonucleoprotein complex assembly), GO:0042273 (ribosomal large subunit biogenesis), GO:0065003 (protein-containing complex assembly), GO:0006396 (RNA processing), GO:0071826 (ribonucleoprotein complex subunit organization), GO:0034470 (ncRNA processing), GO:0000470 (maturation of LSU-rRNA), GO:0006364 (rRNA processing), GO:0034660 (ncRNA metabolic process), GO:0000460 (maturation of 5.8S rRNA), GO:0016072 (rRNA metabolic process), GO:0000463 (maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)), GO:0000466 (maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA))
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:1990904 (ribonucleoprotein complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140513 (nuclear protein-containing complex), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043229 (intracellular organelle), GO:0030684 (preribosome), GO:0043228 (non-membrane-bounded organelle), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0030687 (preribosome, large subunit precursor), GO:0030686 (90S preribosome), GO:0070013 (intracellular organelle lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus), GO:0031981 (nuclear lumen)