Protein: Excitatory amino acid transporter 1 (Glial glutamate transporter) (Sodium-dependent glutamate/aspartate transporter 1) (GLAST) (GLAST-1) (Solute carrier family 1 member 3) (P24942)
Organism: Rattus norvegicus (Rat)
Function: Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (By similarity). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity).
Subcellular location: Cell membrane {ECO:0000269|PubMed:1279699, ECO:0000269|PubMed:8387171}; Multi-pass membrane protein.
Sequence: MTKSNGEEPRMGSRMERFQQGVRKRTLLAKKKVQNITKEDVKSYLFRNAFVLLTVSAVIVGTILGFALRPYKMSYREVKYFSFPGELLMRMLQMLVLPLIISSLVTGMAALDSKASGKMGMRAVVYYMTTTIIAVVIGIIIVIIIHPGKGTKENMYREGKIVQVTAADAFLDLIRNMFPPNLVEACFKQFKTSYEKRSFKVPIQANETLLGAVINNVSEAMETLTRIREEMVPVPGSVNGVNALGLVVFSMCFGFVIGNMKEQGQALREFFDSLNEAIMRLVAVIMWYAPLGILFLIAGKILEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLPLLYFLVTRKNPWVFIGGLLQALITALGTSSSSATLPITFKCLEENNGVDKRITRFVLPVGATINMDGTALYEALAAIFIAQVNNFDLNFGQIITISITATAASIGAAGIPQAGLVTMVIVLTSVGLPTDDITLIIAVDWFLDRLRTTTNVLGDSLGAGIVEHLSRHELKNRDVEMGNSVIEENEMKKPYQLIAQDNEPEKPVADSETKM
InterPro domains: - IPR050746: Dicarboxylate/Amino Acid:Cation Symporter (family) [14-524]
- IPR018107: Sodium:dicarboxylate symporter, conserved site (conserved_site) [84-415]
- IPR001991: Sodium:dicarboxylate symporter (family) [52-496]
- IPR036458: Sodium:dicarboxylate symporter superfamily (homologous_superfamily) [39-506]
Interaction partners: - Excitatory amino acid transporter 2
- Glutamine synthetase
- Glutamate receptor ionotropic, NMDA 2B
- Cytosolic 10-formyltetrahydrofolate dehydrogenase
- Nestin
- Glial fibrillary acidic protein
- Aquaporin-4
- Hexokinase-1
- ATP-sensitive inward rectifier potassium channel 10
- Fatty acid-binding protein, brain
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity), GO:0015075 (monoatomic ion transmembrane transporter activity), GO:0022804 (active transmembrane transporter activity), GO:0005342 (organic acid transmembrane transporter activity), GO:1901702 (salt transmembrane transporter activity), GO:0042887 (amide transmembrane transporter activity), GO:0008514 (organic anion transmembrane transporter activity), GO:0015318 (inorganic molecular entity transmembrane transporter activity), GO:0046943 (carboxylic acid transmembrane transporter activity), GO:0008324 (monoatomic cation transmembrane transporter activity), GO:0015081 (sodium ion transmembrane transporter activity), GO:0015291 (secondary active transmembrane transporter activity), GO:0022890 (inorganic cation transmembrane transporter activity), GO:0022853 (active monoatomic ion transmembrane transporter activity), GO:0005313 (L-glutamate transmembrane transporter activity), GO:0005343 (organic acid:sodium symporter activity), GO:0046873 (metal ion transmembrane transporter activity), GO:0005283 (amino acid:sodium symporter activity), GO:0015293 (symporter activity), GO:0015294 (solute:monoatomic cation symporter activity), GO:0015370 (solute:sodium symporter activity), GO:0015171 (amino acid transmembrane transporter activity), GO:0015179 (L-amino acid transmembrane transporter activity), GO:0015172 (acidic amino acid transmembrane transporter activity), GO:0005416 (amino acid:monoatomic cation symporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0009628 (response to abiotic stimulus), GO:0051641 (cellular localization), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0055085 (transmembrane transport), GO:0042221 (response to chemical), GO:0051234 (establishment of localization), GO:0003008 (system process), GO:0033036 (macromolecule localization), GO:0007267 (cell-cell signaling), GO:0065008 (regulation of biological quality), GO:0009719 (response to endogenous stimulus), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0048523 (negative regulation of cellular process), GO:0007154 (cell communication), GO:0007610 (behavior), GO:0048522 (positive regulation of cellular process), GO:0001101 (response to acid chemical), GO:0001666 (response to hypoxia), GO:0060548 (negative regulation of cell death), GO:0035556 (intracellular signal transduction), GO:0009611 (response to wounding), GO:0009410 (response to xenobiotic stimulus), GO:0009314 (response to radiation), GO:0048731 (system development), GO:0070482 (response to oxygen levels), GO:0048513 (animal organ development), GO:0071495 (cellular response to endogenous stimulus), GO:0051649 (establishment of localization in cell), GO:1901700 (response to oxygen-containing compound), GO:0030534 (adult behavior), GO:0034220 (monoatomic ion transmembrane transport), GO:0010033 (response to organic substance), GO:0009991 (response to extracellular stimulus), GO:0010035 (response to inorganic substance), GO:1903825 (organic acid transmembrane transport), GO:0098739 (import across plasma membrane), GO:0010941 (regulation of cell death), GO:1901698 (response to nitrogen compound), GO:0060322 (head development), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0006810 (transport), GO:0046677 (response to antibiotic), GO:0070727 (cellular macromolecule localization), GO:0050877 (nervous system process), GO:1902074 (response to salt), GO:0010038 (response to metal ion), GO:0071417 (cellular response to organonitrogen compound), GO:0009416 (response to light stimulus), GO:0001504 (neurotransmitter uptake), GO:0071705 (nitrogen compound transport), GO:1901654 (response to ketone), GO:0032355 (response to estradiol), GO:0071453 (cellular response to oxygen levels), GO:0071310 (cellular response to organic substance), GO:1990089 (response to nerve growth factor), GO:1902075 (cellular response to salt), GO:1901699 (cellular response to nitrogen compound), GO:0071702 (organic substance transport), GO:0098810 (neurotransmitter reuptake), GO:0007612 (learning), GO:0006811 (monoatomic ion transport), GO:0015698 (inorganic anion transport), GO:0048678 (response to axon injury), GO:0007613 (memory), GO:0007420 (brain development), GO:0006836 (neurotransmitter transport), GO:0098657 (import into cell), GO:0042220 (response to cocaine), GO:0007399 (nervous system development), GO:0010243 (response to organonitrogen compound), GO:0071241 (cellular response to inorganic substance), GO:0033993 (response to lipid), GO:0031667 (response to nutrient levels), GO:0036293 (response to decreased oxygen levels), GO:0007417 (central nervous system development), GO:0014070 (response to organic cyclic compound), GO:0008104 (protein localization), GO:0014070 (response to organic cyclic compound), GO:0098656 (monoatomic anion transmembrane transport), GO:1905039 (carboxylic acid transmembrane transport), GO:1901701 (cellular response to oxygen-containing compound), GO:0043279 (response to alkaloid), GO:0150104 (transport across blood-brain barrier), GO:0043279 (response to alkaloid), GO:0071285 (cellular response to lithium ion), GO:0043279 (response to alkaloid), GO:0071285 (cellular response to lithium ion), GO:0015711 (organic anion transport), GO:0003333 (amino acid transmembrane transport), GO:0071407 (cellular response to organic cyclic compound), GO:0071248 (cellular response to metal ion), GO:0042886 (amide transport), GO:0071312 (cellular response to alkaloid), GO:0036294 (cellular response to decreased oxygen levels), GO:0042886 (amide transport), GO:0071312 (cellular response to alkaloid), GO:0036294 (cellular response to decreased oxygen levels), GO:0071312 (cellular response to alkaloid), GO:0010043 (response to zinc ion), GO:0006865 (amino acid transport), GO:0015849 (organic acid transport), GO:0006820 (monoatomic anion transport), GO:0051938 (L-glutamate import), GO:0046942 (carboxylic acid transport), GO:0015800 (acidic amino acid transport), GO:0015807 (L-amino acid transport), GO:1902475 (L-alpha-amino acid transmembrane transport), GO:0006835 (dicarboxylic acid transport), GO:0015813 (L-glutamate transmembrane transport), GO:0006813 (potassium ion transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0043198 (dendritic shaft), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0030054 (cell junction), GO:0098794 (postsynapse), GO:0044297 (cell body), GO:0098793 (presynapse), GO:0036477 (somatodendritic compartment), GO:0071944 (cell periphery), GO:0098796 (membrane protein complex), GO:0042734 (presynaptic membrane), GO:0043197 (dendritic spine), GO:0098590 (plasma membrane region), GO:0120025 (plasma membrane bounded cell projection), GO:0097447 (dendritic tree), GO:0045202 (synapse), GO:0005886 (plasma membrane), GO:0043005 (neuron projection), GO:0097060 (synaptic membrane), GO:0097386 (glial cell projection), GO:0030425 (dendrite), GO:0097449 (astrocyte projection), GO:0044309 (neuron spine)