Protein: DnaJ homolog subfamily A member 1 (DnaJ protein homolog 2) (HSDJ) (Heat shock 40 kDa protein 4) (Heat shock protein J2) (HSJ-2) (Human DnaJ protein 2) (hDj-2) (P31689)
Organism: Homo sapiens (Human)
Function: Co-chaperone for HSPA8/Hsc70. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV.
Subcellular location: Membrane {ECO:0000305|PubMed:10816573}; Lipid-anchor {ECO:0000305|PubMed:10816573}. Cytoplasm {ECO:0000269|PubMed:10816573}. Microsome {ECO:0000250}. Nucleus {ECO:0000269|PubMed:10816573}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:10816573}. Mitochondrion {ECO:0000250}. Note=Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei. {ECO:0000250}.
Sequence: MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEKFKQISQAYEVLSDAKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRERRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQCQTS
InterPro domains: - IPR001305: Heat shock protein DnaJ, cysteine-rich domain (domain) [121-205]
- IPR036410: Heat shock protein DnaJ, cysteine-rich domain superfamily (homologous_superfamily) [131-203]
- IPR008971: HSP40/DnaJ peptide-binding (homologous_superfamily) [100-331]
- IPR036869: Chaperone J-domain superfamily (homologous_superfamily) [2-104]
- IPR044713: DnaJ homolog subfamily A member 1/2-like (family) [4-380]
- IPR001623: DnaJ domain (domain) [5-79]
- IPR012724: Chaperone DnaJ (family) [3-376]
- IPR002939: Chaperone DnaJ, C-terminal (domain) [108-329]
- IPR018253: DnaJ domain, conserved site (conserved_site) [45-64]
Interaction partners: - Heat shock cognate 71 kDa protein
- Heat shock 70 kDa protein 4
- Heat shock protein HSP 90-alpha
- Heat shock 70 kDa protein 1A
- Heat shock protein HSP 90-beta
- Heat shock 70 kDa protein 1-like
- Stress-induced-phosphoprotein 1
- Heat shock protein 105 kDa
- DnaJ homolog subfamily B member 1
- DnaJ homolog subfamily A member 4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0051087 (chaperone binding), GO:0051082 (unfolded protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0019222 (regulation of metabolic process), GO:0006457 (protein folding), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0040013 (negative regulation of locomotion), GO:0051241 (negative regulation of multicellular organismal process), GO:0051239 (regulation of multicellular organismal process), GO:0048523 (negative regulation of cellular process), GO:0040012 (regulation of locomotion), GO:0010632 (regulation of epithelial cell migration), GO:0051129 (negative regulation of cellular component organization), GO:2000145 (regulation of cell motility), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0042026 (protein refolding), GO:0010633 (negative regulation of epithelial cell migration), GO:0060255 (regulation of macromolecule metabolic process), GO:0051128 (regulation of cellular component organization), GO:0044087 (regulation of cellular component biogenesis), GO:2000146 (negative regulation of cell motility), GO:0010594 (regulation of endothelial cell migration), GO:0090083 (regulation of inclusion body assembly), GO:0010628 (positive regulation of gene expression), GO:0030334 (regulation of cell migration), GO:0010596 (negative regulation of endothelial cell migration), GO:0010468 (regulation of gene expression), GO:0030336 (negative regulation of cell migration)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0016020 (membrane), GO:0005622 (intracellular anatomical structure)