Protein: LexA repressor 1 (EC 3.4.21.88) (P0A153)
Organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.
Sequence: MLKLTPRQAEILAFIKRCLEDNGFPPTRAEIAQELGFKSPNAAEEHLKALARKGAIEMTPGASRGIRIPGLEAKAEEAGLPIIGRVAAGAPILAEQHIEQSCNINPAFFHPQADYLLRVHGMSMKDVGIFDGDLLAVHTCREARNGQIVVARIGDEVTVKRFKREGSKVWLLAENPEFAPIEVDLKEQELVIEGLSVGVIRR
InterPro domains: - IPR036390: Winged helix DNA-binding domain superfamily (homologous_superfamily) [3-70]
- IPR006197: Peptidase S24, LexA-like (family) [115-166]
- IPR039418: LexA-like (domain) [115-191]
- IPR050077: LexA repressor (family) [1-202]
- IPR006200: Transcription regulator LexA (family) [1-202]
- IPR006199: LexA repressor, DNA-binding domain (domain) [1-65]
- IPR015927: Peptidase S24/S26A/S26B/S26C (domain) [81-194]
- IPR036286: LexA/Signal peptidase-like superfamily (homologous_superfamily) [79-201]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [1-68]
Interaction partners: - Protein RecA
- Cell division inhibitor SulA
- DNA polymerase IV
- DNA linked enzyme involved in DNA repair
- DNA repair protein RecN
- Error-prone DNA polymerase
- UvrABC system protein A
- Translesion DNA synthesis-associated protein ImuA
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003700 (DNA-binding transcription factor activity), GO:0003676 (nucleic acid binding), GO:0001217 (DNA-binding transcription repressor activity), GO:0003677 (DNA binding), GO:0001067 (transcription regulatory region nucleic acid binding), GO:0003690 (double-stranded DNA binding), GO:0043565 (sequence-specific DNA binding), GO:0000976 (transcription cis-regulatory region binding), GO:1990837 (sequence-specific double-stranded DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0048523 (negative regulation of cellular process), GO:0009892 (negative regulation of metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0051253 (negative regulation of RNA metabolic process), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0010468 (regulation of gene expression), GO:2001141 (regulation of RNA biosynthetic process), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0045892 (negative regulation of DNA-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0032993 (protein-DNA complex)