Protein: Envelopment polyprotein (Glycoprotein precursor) (M polyprotein) [Cleaved into: Glycoprotein N (Gn) (Glycoprotein G1); Glycoprotein C (Gc) (Glycoprotein G2)] (Q9E006)
Organism: Andes orthohantavirus (ANDV) (Andes virus)
Function: Forms homotetramers with glycoprotein C at the surface of the virion (By similarity). Attaches the virion to host cell receptors including integrin ITGAV/ITGB3 (By similarity). This attachment induces virion internalization possibly through clathrin-dependent endocytosis and dynamin-independent macropinocytosis (Probable). Mediates the assembly and budding of infectious virus particles through its interaction with the nucleocapsid protein and the viral genome (By similarity). May dysregulate normal immune and endothelial cell responses through an ITAM motif (By similarity). Translocates to mitochondria, binds to host TUFM and recruits MAP1LC3B (By similarity). These interactions induce mitochondrial autophagy and therefore destruction of host MAVS leading to inhibition of type I interferon (IFN) responses (By similarity). Concomitant breakdown of glycoprotein N is apparently prevented by the nucleoprotein that may inhibit Gn-stimulated autophagosome-lysosome fusion (By similarity). Interacts with the viral genomic RNA (By similarity). Inhibits the host RIG-I/TBK1 pathway by disrupting the formation of TBK1-TRAF3 complexes and downstream signaling responses required for IFN-beta transcription.
Subcellular location: [Glycoprotein N]: Virion membrane {ECO:0000250|UniProtKB:P08668}; Multi-pass membrane protein {ECO:0000305}. Host cell surface {ECO:0000250|UniProtKB:P08668}. Host Golgi apparatus membrane {ECO:0000250|UniProtKB:P08668}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P08668}. Host endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P08668}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P08668}. Host mitochondrion {ECO:0000250|UniProtKB:P08668}. Note=Interaction between glycoprotein N and glycoprotein C is essential for proper targeting of glycoprotein N to the host Golgi complex, where virion budding occurs. {ECO:0000250|UniProtKB:P27312}.; SUBCELLULAR LOCATION: [Glycoprotein C]: Virion membrane {ECO:0000250|UniProtKB:P08668}; Single-pass type I membrane protein {ECO:0000305}. Host cell surface {ECO:0000250|UniProtKB:P08668}. Host Golgi apparatus membrane {ECO:0000250|UniProtKB:P08668}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P08668}. Host endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P08668}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P08668}. Note=Budding probably takes place at the host Golgi (Probable). Glycoprotein C cytoplasmic tail is important for efficient Golgi localization (By similarity). {ECO:0000250|UniProtKB:P08668, ECO:0000305}.
Sequence: MEGWYLVVLGVCYTLTLAMPKTIYELKMECPHTVGLGQGYIIGSTELGLISIEAASDIKLESSCNFDLHTTSMAQKSFTQVEWRKKSDTTDTTNAASTTFEAQTKTVNLRGTCILAPELYDTLKKVKKTVLCYDLTCNQTHCQPTVYLIAPVLTCMSIRSCMASVFTSRIQVIYEKTHCVTGQLIEGQCFNPAHTLTLSQPAHTYDTVTLPISCFFTPKKSEQLKVIKTFEGILTKTGCTENALQGYYVCFLGSHSEPLIVPSLEDIRSAEVVSRMLVHPRGEDHDAIQNSQSHLRIVGPITAKVPSTSSTDTLKGTAFAGVPMYSSLSTLVRNADPEFVFSPGIVPESNHSTCDKKTVPITWTGYLPISGEMEKVTGCTVFCTLAGPGASCEAYSENGIFNISSPTCLVNKVQRFRGSEQKINFICQRVDQDVVVYCNGQKKVILTKTLVIGQCIYTFTSLFSLMPDVAHSLAVELCVPGLHGWATVMLLSTFCFGWVLIPAVTLIILKCLRVLTFSCSHYTNESKFKFILEKVKIEYQKTMGSMVCDVCHHECETAKELESHRQSCINGQCPYCMTITEATESALQAHYSICKLTGRFQEALKKSLKKPEVKKGCYRTLGVFRYKSRCYVGLVWCLLLTCEIVIWAASAETPLMESGWSDTAHGVGEIPMKTDLELDFSLPSSSSYSYRRKLTNPANKEESIPFHFQMEKQVIHAEIQPLGHWMDATFNIKTAFHCYGACQKYSYPWQTSKCFFEKDYQYETGWGCNPGDCPGVGTGCTACGVYLDKLKSVGKAYKIISLKYTRKVCIQLGTEQTCKHIDANDCLVTPSVKVCIVGTVSKLQPSDTLLFLGPLEQGGIILKQWCTTSCAFGDPGDIMSTPSGMRCPEHTGSFRKICGFATTPVCEYQGNTISGYKRMMATKDSFQSFNLTEPHITTNKLEWIDPDGNTRDHVNLVLNRDVSFQDLSDNPCKVDLHTQAIEGAWGSGVGFTLTCTVGLTECPSFMTSIKACDLAMCYGSTVTNLARGSNTVKVVGKGGHSGSSFKCCHDTDCSSEGLLASAPHLERVTGFNQIDSDKVYDDGAPPCTFKCWFTKSGEWLLGILNGNWIVVVVLVVILILSIIMFSVLCPRRGHKKTV
InterPro domains: - IPR002534: Hantavirus glycoprotein Gn, head (domain) [28-371]
- IPR048791: Glycoprotein Gc, C-terminal, bunyavirales (domain) [973-1136]
- IPR048790: Glycoprotein Gn, base, hantavirus (domain) [382-483]
- IPR012316: ITAM motif, hantavirus type (domain) [613-647]
- IPR002532: Hantavirus glycoprotein Gc, N-terminal domain (domain) [653-971]
- IPR016402: Envelope glycoprotein precursor, Hantavirus (family) [1-1138]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0044068 (modulation by symbiont of host cellular process), GO:0044403 (biological process involved in symbiotic interaction), GO:0035821 (modulation of process of another organism), GO:0052027 (modulation by symbiont of host signal transduction pathway), GO:0044501 (modulation of signal transduction in another organism), GO:0051701 (biological process involved in interaction with host), GO:0044003 (modulation by symbiont of host process), GO:0052029 (suppression by symbiont of host signal transduction pathway)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0018995 (host cellular component), GO:0033643 (host cell part), GO:0043657 (host cell), GO:0033644 (host cell membrane), GO:0033646 (host intracellular part), GO:0043656 (host intracellular region), GO:0033655 (host cell cytoplasm part), GO:0033647 (host intracellular organelle), GO:0030430 (host cell cytoplasm), GO:0033648 (host intracellular membrane-bounded organelle)