Protein: Cytochrome c oxidase subunit 5A, mitochondrial (Cytochrome c oxidase polypeptide Va) (P11240)
Organism: Rattus norvegicus (Rat)
Function: Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Subcellular location: Mitochondrion inner membrane {ECO:0000250|UniProtKB:P00426}; Peripheral membrane protein {ECO:0000250|UniProtKB:P00426}; Matrix side {ECO:0000250|UniProtKB:P00426}.
Sequence: MLAAALRRCTAAAAARGLLHPVSAPSPAAAVCSIRCYSHGSHETDEEFDARWVTYFNKPDIDAWELRKGMNTLVGYDLVPEPKIIDAALRACRRLNDFASAVRILEVVKDKAGPHKEIYPYVIQELRPTLNELGISTPEELGLDKV
InterPro domains: - IPR003204: Cytochrome c oxidase, subunit Va/VI (family) [1-146]
- IPR036545: Cytochrome c oxidase, subunit Va/VI superfamily (homologous_superfamily) [38-146]
Interaction partners: - Cytochrome c oxidase subunit 3
- Cytochrome c1, heme protein, mitochondrial
- Cytochrome c oxidase subunit 2
- Cytochrome b-c1 complex subunit Rieske, mitochondrial
- Cytochrome c oxidase subunit 7C, mitochondrial
- Cytochrome b-c1 complex subunit 2, mitochondrial
- Cytochrome c oxidase subunit 6C-2
- Cytochrome c oxidase subunit 6A1, mitochondrial
- Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
- Cytochrome c oxidase subunit 5B, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0005215 (transporter activity), GO:0044877 (protein-containing complex binding), GO:0022857 (transmembrane transporter activity), GO:0016491 (oxidoreductase activity), GO:0009055 (electron transfer activity), GO:0015075 (monoatomic ion transmembrane transporter activity), GO:0015318 (inorganic molecular entity transmembrane transporter activity), GO:0016675 (oxidoreductase activity, acting on a heme group of donors), GO:0022804 (active transmembrane transporter activity), GO:0015399 (primary active transmembrane transporter activity), GO:0022890 (inorganic cation transmembrane transporter activity), GO:0022853 (active monoatomic ion transmembrane transporter activity), GO:0004129 (cytochrome-c oxidase activity), GO:0008324 (monoatomic cation transmembrane transporter activity), GO:0015453 (oxidoreduction-driven active transmembrane transporter activity), GO:0015078 (proton transmembrane transporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006099 (tricarboxylic acid cycle), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0006082 (organic acid metabolic process), GO:0019637 (organophosphate metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0006091 (generation of precursor metabolites and energy), GO:0035383 (thioester metabolic process), GO:0006793 (phosphorus metabolic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0006637 (acyl-CoA metabolic process), GO:0043436 (oxoacid metabolic process), GO:0019693 (ribose phosphate metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:0015980 (energy derivation by oxidation of organic compounds), GO:0009259 (ribonucleotide metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0045333 (cellular respiration), GO:0009117 (nucleotide metabolic process), GO:0006163 (purine nucleotide metabolic process), GO:0034032 (purine nucleoside bisphosphate metabolic process), GO:0006104 (succinyl-CoA metabolic process), GO:0033865 (nucleoside bisphosphate metabolic process), GO:0009060 (aerobic respiration), GO:0009150 (purine ribonucleotide metabolic process), GO:0033875 (ribonucleoside bisphosphate metabolic process), GO:0043648 (dicarboxylic acid metabolic process), GO:0006105 (succinate metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098798 (mitochondrial protein-containing complex), GO:0016020 (membrane), GO:0070469 (respirasome), GO:0098796 (membrane protein complex), GO:0031975 (envelope), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0031090 (organelle membrane), GO:0031967 (organelle envelope), GO:0005746 (mitochondrial respirasome), GO:0098803 (respiratory chain complex), GO:0043229 (intracellular organelle), GO:0070069 (cytochrome complex), GO:0098800 (inner mitochondrial membrane protein complex), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0005740 (mitochondrial envelope), GO:0043231 (intracellular membrane-bounded organelle), GO:0045277 (respiratory chain complex IV), GO:0019866 (organelle inner membrane), GO:0005751 (mitochondrial respiratory chain complex IV), GO:0031966 (mitochondrial membrane), GO:0005743 (mitochondrial inner membrane)