Protein: 5'-AMP-activated protein kinase catalytic subunit alpha-2 (AMPK subunit alpha-2) (EC 2.7.11.1) (Q95ZQ4)
Organism: Caenorhabditis elegans
Function: Acts as a sensor that couples lifespan to information about energy levels and insulin-like signals. Role in motility and response to oxidative stress. Involved in the establishment of germline stem cell (GSC) quiescence during dauer development. Plays a role in axon regrowth after axotomy in PLM neurons. Plays a role in the maintenance of glycogen stores which are necessary for resistance to hyperosmotic stress. Plays a role in the regulation of flp-7 secretion from ASI neurons. Keeps the CREB-regulated transcription coactivator 1 homolog crtc-1 inactive which in turn inhibits flp-7 secretion. Following serotonin signaling, derepresses crtc-1 which stimulates flp-7 secretion and subsequent body fat loss.
Sequence: MFSHQDRDRDRKEDGGGDGTEMKSKSRSQPSGLNRVKNLSRKLSAKSRKERKDRDSTDNSSKMSSPGGETSTKQQQELKAQIKIGHYILKETLGVGTFGKVKVGIHETTQYKVAVKILNRQKIKSLDVVGKIRREIQNLSLFRHPHIIRLYQVISTPSDIFMIMEHVSGGELFDYIVKHGRLKTAEARRFFQQIISGVDYCHRHMVVHRDLKPENLLLDEQNNVKIADFGLSNIMTDGDFLRTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYALLCGTLPFDDEHVPSLFRKIKSGVFPTPDFLERPIVNLLHHMLCVDPMKRATIKDVIAHEWFQKDLPNYLFPPINESEASIVDIEAVREVTEFQRYHVAEEEVTSALLGDDPHHHLSIAYNLIVDNKRIADETAKLSIEEFYQVTPNKGPGPVHRHPERIAASVSSKITPTLDNTEASGANRNKRAKWHLGIRSQSRPEDIMFEVFRAMKQLDMEWKVLNPYHVIVRRKPDAPAADPPKMSLQLYQVDQRSYLLDFKSLADEESGSASASSSRHASMSMPQKPAGIRGTRTSSMPQAMSMEASIEKMEVHDFSDMSCDVTPPPSPGGAKLSQTMQFFEICAALIGTLAR
InterPro domains: - IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [84-340]
- IPR032270: AMPK, C-terminal adenylate sensor domain (domain) [464-554]
- IPR028375: KA1 domain/Ssp2, C-terminal (homologous_superfamily) [397-625]
- IPR008271: Serine/threonine-protein kinase, active site (active_site) [206-218]
- IPR017441: Protein kinase, ATP binding site (binding_site) [93-116]
- IPR000719: Protein kinase domain (domain) [87-339]
- IPR049020: PRKAA1/2, autoinhibitory domain (domain) [359-406]
Interaction partners: - Association with the SNF1 complex
- CBS domain-containing protein
- 5'-AMP-activated protein kinase subunit beta-1
- Forkhead box protein O
- Target of rapamycin homolog
- CBS domain-containing protein
- DAF-15
- Serine/threonine-protein kinase par-4
- Ribosomal protein S6 kinase beta
- cAMP-dependent protein kinase catalytic subunit
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0004672 (protein kinase activity), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004679 (AMP-activated protein kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0048869 (cellular developmental process), GO:0051641 (cellular localization), GO:0048856 (anatomical structure development), GO:0023057 (negative regulation of signaling), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0032879 (regulation of localization), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0033036 (macromolecule localization), GO:0009791 (post-embryonic development), GO:0044237 (cellular metabolic process), GO:0051235 (maintenance of location), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0009893 (positive regulation of metabolic process), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0006950 (response to stress), GO:0048523 (negative regulation of cellular process), GO:0048585 (negative regulation of response to stimulus), GO:0007154 (cell communication), GO:0044238 (primary metabolic process), GO:0040012 (regulation of locomotion), GO:0048522 (positive regulation of cellular process), GO:0009968 (negative regulation of signal transduction), GO:0010648 (negative regulation of cell communication), GO:1903829 (positive regulation of protein localization), GO:1901564 (organonitrogen compound metabolic process), GO:0032388 (positive regulation of intracellular transport), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0060341 (regulation of cellular localization), GO:0019538 (protein metabolic process), GO:0009966 (regulation of signal transduction), GO:0060255 (regulation of macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0006793 (phosphorus metabolic process), GO:0010876 (lipid localization), GO:0009991 (response to extracellular stimulus), GO:0009894 (regulation of catabolic process), GO:0042594 (response to starvation), GO:0019915 (lipid storage), GO:0010646 (regulation of cell communication), GO:0031325 (positive regulation of cellular metabolic process), GO:0033554 (cellular response to stress), GO:0070727 (cellular macromolecule localization), GO:0031323 (regulation of cellular metabolic process), GO:0016043 (cellular component organization), GO:0031668 (cellular response to extracellular stimulus), GO:0002164 (larval development), GO:0080090 (regulation of primary metabolic process), GO:0071496 (cellular response to external stimulus), GO:0009896 (positive regulation of catabolic process), GO:0042177 (negative regulation of protein catabolic process), GO:0042176 (regulation of protein catabolic process), GO:0051246 (regulation of protein metabolic process), GO:0009267 (cellular response to starvation), GO:0010506 (regulation of autophagy), GO:0006796 (phosphate-containing compound metabolic process), GO:0032880 (regulation of protein localization), GO:1902532 (negative regulation of intracellular signal transduction), GO:1902115 (regulation of organelle assembly), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0010508 (positive regulation of autophagy), GO:0031667 (response to nutrient levels), GO:0051247 (positive regulation of protein metabolic process), GO:0045732 (positive regulation of protein catabolic process), GO:0031331 (positive regulation of cellular catabolic process), GO:0008104 (protein localization), GO:0031329 (regulation of cellular catabolic process), GO:0002119 (nematode larval development), GO:0031669 (cellular response to nutrient levels), GO:1902531 (regulation of intracellular signal transduction), GO:0033365 (protein localization to organelle), GO:1901796 (regulation of signal transduction by p53 class mediator), GO:0030162 (regulation of proteolysis), GO:0006468 (protein phosphorylation), GO:0016310 (phosphorylation), GO:0051898 (negative regulation of protein kinase B signaling), GO:0040024 (dauer larval development), GO:1901800 (positive regulation of proteasomal protein catabolic process), GO:2000060 (positive regulation of ubiquitin-dependent protein catabolic process), GO:0061136 (regulation of proteasomal protein catabolic process), GO:2000008 (regulation of protein localization to cell surface), GO:2000058 (regulation of ubiquitin-dependent protein catabolic process), GO:0009062 (fatty acid catabolic process), GO:1903052 (positive regulation of proteolysis involved in protein catabolic process), GO:0032434 (regulation of proteasomal ubiquitin-dependent protein catabolic process), GO:1903050 (regulation of proteolysis involved in protein catabolic process), GO:0032436 (positive regulation of proteasomal ubiquitin-dependent protein catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0044297 (cell body), GO:0036477 (somatodendritic compartment), GO:0005737 (cytoplasm), GO:0043025 (neuronal cell body), GO:0120025 (plasma membrane bounded cell projection), GO:0097447 (dendritic tree), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043005 (neuron projection), GO:0043231 (intracellular membrane-bounded organelle), GO:0030425 (dendrite), GO:0030424 (axon), GO:0005634 (nucleus)