Protein: DNA-directed RNA polymerase III subunit RPC8 (RNA polymerase III subunit C8) (DNA-directed RNA polymerase III 25 kDa polypeptide) (RNA polymerase III subunit C25) (P35718)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNA. The RPC25/RPC8-RPC17/RPC9 subcomplex may bind Pol III transcripts emerging from the adjacent exit pore during elongation.
Subcellular location: Nucleus.
Sequence: MFILSKIADLVRIPPDQFHRDTISAITHQLNNKFANKIIPNVGLCITIYDLLTVEEGQLKPGDGSSYINVTFRAVVFKPFLGEIVTGWISKCTAEGIKVSLLGIFDDIFIPQNMLFEGCYYTPEESAWIWPMDEETKLYFDVNEKIRFRIEREVFVDVKPKSPKERELEERAQLENEIEGKNEETPQNEKPPAYALLGSCQTDGMGLVSWWE
InterPro domains: - IPR004519: DNA-directed RNA polymerase, subunit E/RPC8 (family) [1-184]
- IPR036898: RNA polymerase Rpb7-like, N-terminal domain superfamily (homologous_superfamily) [1-80]
- IPR005576: RNA polymerase Rpb7-like , N-terminal (domain) [9-64]
- IPR012340: Nucleic acid-binding, OB-fold (homologous_superfamily) [79-212]
- IPR013238: RNA polymerase III, subunit Rpc25 (domain) [83-211]
- IPR045113: RNA polymerase subunit Rpb7-like (family) [1-172]
Interaction partners: - DNA-directed RNA polymerases I, II, and III subunit RPABC5
- DNA-directed RNA polymerase III subunit RPC1
- DNA-directed RNA polymerase III subunit RPC5
- DNA-directed RNA polymerase III subunit RPC10
- DNA-directed RNA polymerase III subunit RPC9
- DNA-directed RNA polymerase III subunit RPC2
- DNA-directed RNA polymerase III subunit RPC3
- DNA-directed RNA polymerase III subunit RPC4
- DNA-directed RNA polymerases I, II, and III subunit RPABC3
- DNA-directed RNA polymerases I, II, and III subunit RPABC1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:1901363 (heterocyclic compound binding), GO:0016740 (transferase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0140098 (catalytic activity, acting on RNA), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0031369 (translation initiation factor binding), GO:0097747 (RNA polymerase activity), GO:0016779 (nucleotidyltransferase activity), GO:0003677 (DNA binding), GO:0003723 (RNA binding), GO:0003727 (single-stranded RNA binding), GO:0003697 (single-stranded DNA binding), GO:0034062 (5'-3' RNA polymerase activity), GO:0003968 (RNA-dependent RNA polymerase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044085 (cellular component biogenesis), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0044249 (cellular biosynthetic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0043933 (protein-containing complex organization), GO:0010467 (gene expression), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0022607 (cellular component assembly), GO:0016070 (RNA metabolic process), GO:0065003 (protein-containing complex assembly), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006352 (DNA-templated transcription initiation), GO:0006351 (DNA-templated transcription), GO:0006353 (DNA-templated transcription termination), GO:0034660 (ncRNA metabolic process), GO:0006384 (transcription initiation at RNA polymerase III promoter), GO:0016072 (rRNA metabolic process), GO:0098781 (ncRNA transcription), GO:0006383 (transcription by RNA polymerase III), GO:0009303 (rRNA transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140535 (intracellular protein-containing complex), GO:1902494 (catalytic complex), GO:0140513 (nuclear protein-containing complex), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0099080 (supramolecular complex), GO:0030880 (RNA polymerase complex), GO:0036464 (cytoplasmic ribonucleoprotein granule), GO:1990234 (transferase complex), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0035770 (ribonucleoprotein granule), GO:0043233 (organelle lumen), GO:0055029 (nuclear DNA-directed RNA polymerase complex), GO:0016591 (RNA polymerase II, holoenzyme), GO:0043227 (membrane-bounded organelle), GO:0000428 (DNA-directed RNA polymerase complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0000932 (P-body), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0061695 (transferase complex, transferring phosphorus-containing groups), GO:0005665 (RNA polymerase II, core complex), GO:0070013 (intracellular organelle lumen), GO:0005634 (nucleus), GO:0031981 (nuclear lumen)