Protein: UDP-glucose:glycoprotein glucosyltransferase (EC 2.4.1.-) (EMS-mutagenized BRI1 suppressor 1) (Protein PRIORITY IN SWEET LIFE 2) (Q0WL80)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation. Required for elongation factor Tu receptor (EFR), but not flagellin-sensing 2 (FLS2) signaling.
Subcellular location: Endoplasmic reticulum lumen {ECO:0000305}.
Sequence: MGTTTNLRSWLYLILLFIVVVGVNAQNRRPKNVQVAVKAKWQGTPLLLEAGELISKESKQLFWEFTDAWLGSDGDDSDCKSARDCLLKISKQASTLLAQPVASLFHFSLTLRSASPRLVLYRQLADESLSSFPHGDDPSATGCCWVDTGSSLFYDVADLQSWLASAPAVGDAVQGPELFDFDHVHFDSRAGSPVAVLYGAVGTDCFRKFHLSLAKAAKEGKVTYVVRPVLPLGCEGKTRPCGAIGARDNVSLAGYGVELALKNMEYKAMDDSAIKKGITLEDPRTEDLSQDVRGFIFSKILDRKPELRSEVMAFRDYLLSSTVSDTLDVWELKDLGHQTAQRIVHASDPLQSMQEINQNFPSVVSSLSRMKLNESIKDEILSNQRMVPPGKALLALNGALLNIEDIDLYMLMDLAHQELSLANHFSKLKIPDGAIRKLLLTTPLPEPDSYRVDFRSVHVTYLNNLEEDDMYKRWRSNINEILMPAFPGQLRYIRKNLFHAVYVIDPATACGLESIETLRSLYENQLPVRFGVILYSTQLIKTIENNGGQIPSSDAVTNAQVKEDLSTMVIRLFLYIKEHHGIQTAFQFLGNLNTLRTESADSSEADIEQEHVDGAFVETILPKVKTLPQDILLKLRQEHTLKEASEASSMFVFKLGLAKLKCSFLMNGLVFDSVEEETLLNAMNEELPKIQEQVYYGQIESHTKVLDKLLSESGLSRYNPQIISGGKNKPRFVSLASSTRKGESMLNDVNYLHSPETSEDVKYVTHLLAADVATKKGMKLLHEGVRYLIGGSKSARLGVLFSSSQNADPHSLLFIKFFEKTASSFSHKEKVLYFLDKLCLFYEREYLLKTSVESASSQMFIDKVLELADEYGLSSKAYRSCLVESVDEELLKRLTKVAQFLSWELGLESDANAIISNGRVIFPVDERTFLGQDLHLLESMEFNQRVKPVQEIIEGIEWQDVDPDLLTSKYFSDVFMFVSSAMATRDRSSESARFEVLNSEYSAVLLGNENATIHIDAVIDPLSPTGQKLASLLQVLQKHVQTSMRIVLNPMSSLVDIPLKNYYRYVLPNTDDYSSTGFDVDGPKAFFANMPLSKTLTMNLDVPEPWLVEPVIAIHDLDNILLENLGDTTTLQAVFEVESLVLTGHCAEKDHEAPRGLQLILGTKNRPHLVDTLVMANLGYWQMKVSPGVWYLQLAPGRSSELYALKGGNDGSQDQSSLKRITIDDLRGKVVHLEVVKRKGKEHEKLLVPSDGDDAVQQNKEGSWNSNFLKWASGFVGGRQQSMKGGPDKEHEKGGRQGKTINIFSIASGHLYERFLKIMILSVLKNTNRPVKFWFIKNYLSPQFKDVIPHMAQEYNFEYELITYKWPSWLHKQKEKQRIIWAYKILFLDVIFPLSLEKVIFVDADQIIRTDMGELYDMDIKGRPLAYTPFCDNNREMDGYKFWKQGFWKEHLRGRPYHISALYVVDLVKFRETAAGDNLRVFYETLSKDPNSLSNLDQDLPNYAQHTVPIFSLPQEWLWCESWCGNATKAKARTIDLCNNPMTKEPKLQGARRIVTEWPDLDLEARKFTAKILGEDVELNEPVAAPATDKPNPLPSNDISEDTEQDLESKAEL
InterPro domains: - IPR040693: UGGT, thioredoxin-like domain 1 (domain) [44-235]
- IPR040525: UDP-glucose:glycoprotein glucosyltransferase, thioredoxin-like domain 4 (domain) [739-983]
- IPR029044: Nucleotide-diphospho-sugar transferases (homologous_superfamily) [1301-1547]
- IPR040694: UGGT, thioredoxin-like domain 2 (domain) [325-442]
- IPR009448: UDP-glucose:Glycoprotein Glucosyltransferase (family) [15-1600]
- IPR040497: Glucosyltransferase 24, catalytic domain (domain) [1301-1567]
- IPR040692: UGGT, thioredoxin-like domain 3 (domain) [453-722]
Interaction partners: - Calnexin homolog 2
- Calnexin homolog 1
- Calreticulin-3
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A
- Calreticulin-2
- Calreticulin-1
- Protein IMPAIRED IN BABA-INDUCED STERILITY 1
- Protein OS-9 homolog
- Probable glucan 1,3-alpha-glucosidase
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016757 (glycosyltransferase activity), GO:0016758 (hexosyltransferase activity), GO:0008194 (UDP-glycosyltransferase activity), GO:0046527 (glucosyltransferase activity), GO:0035251 (UDP-glucosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0040007 (growth), GO:0009987 (cellular process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0022414 (reproductive process), GO:0048589 (developmental growth), GO:0009856 (pollination), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0044706 (multi-multicellular organism process), GO:0009653 (anatomical structure morphogenesis), GO:0016049 (cell growth), GO:0003006 (developmental process involved in reproduction), GO:0009860 (pollen tube growth), GO:0000902 (cell morphogenesis), GO:0048868 (pollen tube development), GO:0048588 (developmental cell growth), GO:0048468 (cell development), GO:0060560 (developmental growth involved in morphogenesis), GO:0030154 (cell differentiation), GO:0009826 (unidimensional cell growth), GO:0009932 (cell tip growth), GO:0000904 (cell morphogenesis involved in differentiation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)