Protein: E3 ubiquitin-protein ligase MAG2 (Q06436)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: E3 ubiquitin-protein ligase involved in the degradation of non-functional 18S rRNAs in response to stalled ribosomes. Catalyzes monoubiquitination of RPS3/uS3 in response to stalled ribosomes, initiating a HEL2-dependent response that activates the degradation of non-functional 18S rRNAs
Subcellular location: Cytoplasm
Sequence: MVEPDMQKKASGGSGGSEMDTLNATSNSSKQGVSNNKRNPVSKKKPGNKVSDGRDNAHNYHGEGRRKSSKQQRSRTPYKETSTRINDQDIDLSIQEEILGGNFKLRGRKTQVSINHLLNFQLPEVEREKSRSSSSKKSNRRRDEHVHLHGDTFVNVNYRLLVDDRFDYPEQNCNPNVPVDQEKILRVIVPKGQNCSICLSEEPVAPRMVTCGHIFCLSCLLNFFSIEETVKNKETGYSKKKKYKECPLCGSIIGPKRVKPVLYEDDFDVTRLNQKPEPGATVHLQLMCKPHGSLLPLPVALHLDPLKCGNFPPANLGSIKHYAHIMKCGVSYSLELYQKDIVAIQEQYEIDKAIYNDSGKFVKQSIENINDQISTLLAATTDLSPLSNDINNGLDNFHFDDDLLTKYDDSSAYFFYQTLVASSTKYFLSPLDVKILLTIFHYYSKFPESIETTVENIHYDTVVTEQLIRRYKYIGHLPIGTEIALLDLDWRKIPFLPKEIYEQFAHELKQRRRKFTMKKQKEDKEKKLYEKRLEQEHAEFYRKENGNSLKFEDSVQMATHYESIVSSSIPLNSLGISMLGPPTNSCSTPQKQAPSHTKRTIWGTSIAVTEDEKASKENKEFQDMLLQRIRQEDSSDVTDSTDSPPTSNGKRGRKKKGKVMLFSSNHQALG
InterPro domains: - IPR001841: Zinc finger, RING-type (domain) [195-249]
- IPR039739: RING-finger protein MAG2/RNF10 (family) [20-666]
- IPR017907: Zinc finger, RING-type, conserved site (conserved_site) [211-220]
- IPR027370: Zinc finger, RING-type, eukaryotic (domain) [195-247]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [180-275]
Interaction partners: - Uncharacterized protein YKR073C
- Uncharacterized RING finger protein YBR062C
- Uncharacterized protein YMR075C-A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0019787 (ubiquitin-like protein transferase activity), GO:0004842 (ubiquitin-protein transferase activity), GO:0061659 (ubiquitin-like protein ligase activity), GO:0061630 (ubiquitin protein ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0019538 (protein metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:0036211 (protein modification process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0043412 (macromolecule modification), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0010468 (regulation of gene expression), GO:1902680 (positive regulation of RNA biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0070647 (protein modification by small protein conjugation or removal), GO:0006355 (regulation of DNA-templated transcription), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0032446 (protein modification by small protein conjugation), GO:0016567 (protein ubiquitination), GO:0051865 (protein autoubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)