Protein: Putative mitochondrial cysteine synthase (CS) (EC 2.5.1.47) (Cysteine synthase-like protein) (CSl) (O-acetylserine (thiol)-lyase) (OAS-TL) (O-acetylserine sulfhydrylase) (P53206)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Putative cysteine synthase that catalyzes the conversion of O-acetyl-L-serine (OAS) into cysteine, the last step in the cysteine biosynthesis pathway. However, this CS-like protein is unlikely to function in cysteine biosynthesis. It seems that in S.cerevisiae cysteine biosynthesis occurs exclusively through the cystathionine pathway and not via direct incorporation of sulfur into OAS.
Subcellular location: Mitochondrion {ECO:0000269|PubMed:14562095}. Mitochondrion outer membrane {ECO:0000269|PubMed:16407407}; Single-pass membrane protein {ECO:0000255}. Note=Selective removal from mitochondrial outer membrane is achieved by sorting into a mitochondrial-derived compartment, or MDC, followed by release through mitochondrial fission and elimination by autophagy. {ECO:0000269|PubMed:27097106}.
Sequence: MSCSQNKTSVSLAWRECISIASVLIGAYASYKYYKLFKTRDIPRPKEGVEELIGNTPLVKIRSLTKATGVNIYAKLELCNPAGSAKDRVALNIIKTAEELGELVRGEPGWVFEGTSGSTGISIAVVCNALGYRAHISLPDDTSLEKLALLESLGATVNKVKPASIVDPNQYVNAAKKACNELKKSGNGIRAVFADQFENEANWKVHYQTTGPEIAHQTKGNIDAFIAGCGTGGTITGVAKFLKERAKIPCHVVLADPQGSGFYNRVNYGVMYDYVEKEGTRRRHQVDTIVEGIGLNRITHNFHMGEKFIDESIRVNDNQAIRMAKYLSVNDGLFVGSSTAINAVAAIQVAKTLPHGSNIVIIACDSGSRHLSKFWKEAKEIDHDVSLEEVINI
InterPro domains: - IPR001926: Tryptophan synthase beta chain-like, PALP domain (domain) [50-365]
- IPR050214: Cysteine synthase/Cystathionine beta-synthase (family) [48-377]
- IPR001216: Cysteine synthase/cystathionine beta-synthase, pyridoxal-phosphate attachment site (binding_site) [75-93]
- IPR036052: Tryptophan synthase beta chain-like, PALP domain superfamily (homologous_superfamily) [34-376]
Interaction partners: - Homocysteine/cysteine synthase
- Sulfite reductase subunit beta
- Homocysteine/cysteine synthase
- Cystathionine gamma-lyase
- Putative cystathionine gamma-synthase YML082W
- Cystathionine gamma-synthase
- Cystathionine beta-lyase
- Sulfite reductase flavoprotein component
- Putative cystathionine beta-lyase
- Adenosylhomocysteinase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0005515 (protein binding), GO:0016829 (lyase activity), GO:0016846 (carbon-sulfur lyase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0000096 (sulfur amino acid metabolic process), GO:0044272 (sulfur compound biosynthetic process), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0006534 (cysteine metabolic process), GO:0000097 (sulfur amino acid biosynthetic process), GO:0009069 (serine family amino acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0009070 (serine family amino acid biosynthetic process), GO:0019344 (cysteine biosynthetic process), GO:0006563 (L-serine metabolic process), GO:0006535 (cysteine biosynthetic process from serine)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0031090 (organelle membrane), GO:0031967 (organelle envelope), GO:0005739 (mitochondrion), GO:0043229 (intracellular organelle), GO:0019867 (outer membrane), GO:0043227 (membrane-bounded organelle), GO:0005740 (mitochondrial envelope), GO:0031968 (organelle outer membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0031966 (mitochondrial membrane), GO:0005741 (mitochondrial outer membrane)