Protein: Paraclostridial mosquitocidal protein 1 (A0A5P3XKQ1)
Organism: Paraclostridium bifermentans (Clostridium bifermentans)
Function: [Paraclostridial mosquitocidal protein 1]: Neurotoxin active against Anopheles but not Aedes mosquitoes upon oral ingestion; expression of the ptox operon (ntnh-orfX1-orfX2-orfX3-pmp1) in B.thuringiensis kills Anopheles but not Aedes mosquito 3rd instar larvae. The ntnh-pmp1 construct is about half as toxic. PMP1 is toxic when injected directly into Anopheles or Aedes mosquito 3rd instar larvae, larvae no longer move, suggesting they are paralyzed. Adult mosquitoes (Anopheles or Aedes) and Drosophila lose the ability to fly in a dose-dependent manner by 24 hours after injection with 100 pg neurotoxin. Not toxic upon injection in mice
Sequence: MLQIRVFNYNDPIDGENIVELRYHNRSPVKAFQIVDGIWIIPERYNFTNDTKKVPDDRALTILEDEVFAVRENDYLTTDVNEKNSFLNNITKLFKRINSSNIGNQLLNYISTSVPYPVVSTNSIKARDYNTIKFDSIDGRRITKSANVLIYGPSMKNLLDKQTRAINGEEAKNGIGCLSDIIFSPNYLSVQTVSSSRFVEDPASSLTHELIHALHNLYGIQYPGEEKFKFGGFIDKLLGTRECIDYEEVLTYGGKDSEIIRKKIDKSLYPDDFVNKYGEMYKRIKGSNPYYPDEKKLKQSFLNRMNPFDQNGTFDTKEFKNHLMDLWFGLNESEFAKEKKILVRKHYITKQINPKYTELTNDVYTEDKGFVNGQSIDNQNFKIIDDLISKKVKLCSITSKNRVNICIDVNKEDLYFISDKEGFENIDFSEPEIRYDSNVTTATTSSFTDHFLVNRTFNDSDRFPPVELEYAIEPAEIVDNTIMPDIDQKSEISLDNLTTFHYLNAQKMDLGFDSSKEQLKMVTSIEESLLDSKKVYTPFTRTAHSVNERISGIAESYLFYQWLKTVINDFTDELNQKSNTDKVADISWIIPYVGPALNIGLDLSHGDFTKAFEDLGVSILFAIAPEFATISLVALSIYENIEEDSQKEKVINKVENTLARRIEKWHQVYAFMVAQWWGMVHTQIDTRIHQMYESLSHQIIAIKANMEYQLSHYKGPDNDKLLLKDYIYEAEIALNTSANRAMKNIERFMIESSISYLKNNLIPSVVENLKKFDADTKKNLDQFIDKNSSVLGSDLHILKSQVDLELNPTTKVAFNIQSIPDFDINALIDRLGIQLKDNLVFSLGVESDKIKDLSGNNTNLEVKTGVQIVDGRDSKTIRLNSNENSSIIVQKNESINFSYFSDFTISFWIRVPRLNKNDFIDLGIEYDLVNNMDNQGWKISLKDGNLVWRMKDRFGKIIDIITSLTFSNSFIDKYISSNIWRHITITVNQLKDCTLYINGDKIDSKSINELRGIDNNSPIIFKLEGNRNKNQFIRLDQFNIYQRALNESEVEMLFNSYFNSNILRDFWGEPLEYNKSYYMINQAILGGPLRSTYKSWYGEYYPYISRMRTFNVSSFILIPYLYHKGSDVEKVKIINKNNVDKYVRKNDVADVKFENYGNLILTLPMYSKIKERYMVLNEGRNGDLKLIQLQSNDKYYCQIRIFEMYRNGLLSIADDENWLYSSGWYLYSSGWYLDNYKTLDLKKHTKTNWYFVSEDEGWKE
InterPro domains: - IPR036248: Clostridium neurotoxin, translocation domain (homologous_superfamily) [403-817]
- IPR013320: Concanavalin A-like lectin/glucanase domain superfamily (homologous_superfamily) [835-1045]
- IPR011065: Kunitz inhibitor STI-like superfamily (homologous_superfamily) [1046-1260]
- IPR000395: Botulinum/Tetanus toxin, catalytic chain (domain) [6-371]
- IPR012928: Clostridium neurotoxin, receptor binding N-terminal (domain) [848-1045]
- IPR012500: Clostridium neurotoxin, translocation (domain) [498-814]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0005215 (transporter activity), GO:0016787 (hydrolase activity), GO:0022857 (transmembrane transporter activity), GO:0043167 (ion binding), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0140318 (protein transporter activity), GO:0008320 (protein transmembrane transporter activity), GO:0008233 (peptidase activity), GO:0022884 (macromolecule transmembrane transporter activity), GO:0043169 (cation binding), GO:0008237 (metallopeptidase activity), GO:0004175 (endopeptidase activity), GO:0046872 (metal ion binding), GO:0004222 (metalloendopeptidase activity), GO:0046914 (transition metal ion binding), GO:0008270 (zinc ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0065008 (regulation of biological quality), GO:0023051 (regulation of signaling), GO:0051051 (negative regulation of transport), GO:0048523 (negative regulation of cellular process), GO:0032879 (regulation of localization), GO:0050794 (regulation of cellular process), GO:0001505 (regulation of neurotransmitter levels), GO:1903530 (regulation of secretion by cell), GO:0099177 (regulation of trans-synaptic signaling), GO:0051049 (regulation of transport), GO:0010646 (regulation of cell communication), GO:0051048 (negative regulation of secretion), GO:1903531 (negative regulation of secretion by cell), GO:0051588 (regulation of neurotransmitter transport), GO:0051046 (regulation of secretion), GO:0046928 (regulation of neurotransmitter secretion), GO:0050804 (modulation of chemical synaptic transmission)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0071944 (cell periphery), GO:0005737 (cytoplasm), GO:0005576 (extracellular region), GO:0031090 (organelle membrane), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0030139 (endocytic vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0012506 (vesicle membrane), GO:0030135 (coated vesicle), GO:0031982 (vesicle), GO:0030659 (cytoplasmic vesicle membrane), GO:0030666 (endocytic vesicle membrane), GO:0097708 (intracellular vesicle), GO:0030136 (clathrin-coated vesicle), GO:0030662 (coated vesicle membrane), GO:0045334 (clathrin-coated endocytic vesicle), GO:0030669 (clathrin-coated endocytic vesicle membrane), GO:0030665 (clathrin-coated vesicle membrane)