Protein: Amidophosphoribosyltransferase 2, chloroplastic (AtATase2) (AtPURF2) (PRPP2) (EC 2.4.2.14) (Glutamine phosphoribosylpyrophosphate amidotransferase 2) (AtGPRAT2) (Protein CHLOROPLAST IMPORT APPARATUS 1) (Protein DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS) (Q9STG9)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Catalyzes the first committed step of 'de novo purine biosynthesis from glutamine. Required for chloroplast biogenesis and cell division. Confers sensitivity to the phenyltriazole acetic acid compound [5-(4-chlorophenyl)-1-isopropyl-1H-[1,2,4]triazol-3-yl]-acetic acid (DAS734), a bleaching herbicide.
Subcellular location: Plastid, chloroplast stroma {ECO:0000269|PubMed:15266056, ECO:0000269|PubMed:18431481}.
Sequence: MAATSSISSSLSLNAKPNKLSNNNNNNKPHRFLRNPFLNPSSSSFSPLPASISSSSSSPSFPLRVSNPLTLLAADNDDYDEKPREECGVVGIYGDSEASRLCYLALHALQHRGQEGAGIVTVSKDKVLQTITGVGLVSEVFSESKLDQLPGDIAIGHVRYSTAGSSMLKNVQPFVAGYRFGSVGVAHNGNLVNYTKLRADLEENGSIFNTSSDTEVVLHLIAISKARPFFMRIVDACEKLQGAYSMVFVTEDKLVAVRDPHGFRPLVMGRRSNGAVVFASETCALDLIEATYEREVYPGEVLVVDKDGVKCQCLMPHPEPKQCIFEHIYFSLPNSIVFGRSVYESRHVFGEILATESPVDCDVVIAVPDSGVVAALGYAAKAGVAFQQGLIRSHYVGRTFIEPSQKIRDFGVKLKLSPVRGVLEGKRVVVVDDSIVRGTTSSKIVRLLREAGAKEVHMRIASPPIIASCYYGVDTPSSNELISNRMSVDEIRDYIGCDSLAFLSFETLKKHLGEDSRSFCYACFTGDYPVKPTEDKVKRGGDFIDDGLVGGIHNIEGGWVR
InterPro domains: - IPR029055: Nucleophile aminohydrolases, N-terminal (homologous_superfamily) [87-529]
- IPR000836: Phosphoribosyltransferase domain (domain) [350-461]
- IPR029057: Phosphoribosyltransferase-like (homologous_superfamily) [323-536]
- IPR017932: Glutamine amidotransferase type 2 domain (domain) [87-307]
- IPR005854: Amidophosphoribosyltransferase (family) [83-542]
- IPR035584: Amidophosphoribosyltransferase, N-terminal (domain) [87-338]
Interaction partners: - Phosphoribosylamine--glycine ligase, chloroplastic
- Carbamoyl phosphate synthase arginine-specific large chain, chloroplastic
- Carbamoyl phosphate synthase small chain, chloroplastic
- Probable phosphoribosylformylglycinamidine synthase, chloroplastic/mitochondrial
- Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic
- Ribose-phosphate pyrophosphokinase 1, chloroplastic
- Ribose-phosphate pyrophosphokinase 5, chloroplastic
- Ribose-phosphate pyrophosphokinase 2, chloroplastic
- Glutamine synthetase cytosolic isozyme 1-5
- Glutamine synthetase cytosolic isozyme 1-4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016757 (glycosyltransferase activity), GO:0016763 (pentosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0006793 (phosphorus metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0019693 (ribose phosphate metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0090407 (organophosphate biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0009259 (ribonucleotide metabolic process), GO:0009123 (nucleoside monophosphate metabolic process), GO:0009117 (nucleotide metabolic process), GO:0046390 (ribose phosphate biosynthetic process), GO:0006163 (purine nucleotide metabolic process), GO:0072522 (purine-containing compound biosynthetic process), GO:1901293 (nucleoside phosphate biosynthetic process), GO:0009126 (purine nucleoside monophosphate metabolic process), GO:0009124 (nucleoside monophosphate biosynthetic process), GO:0009165 (nucleotide biosynthetic process), GO:0006164 (purine nucleotide biosynthetic process), GO:0009150 (purine ribonucleotide metabolic process), GO:0009260 (ribonucleotide biosynthetic process), GO:0009161 (ribonucleoside monophosphate metabolic process), GO:0009152 (purine ribonucleotide biosynthetic process), GO:0009156 (ribonucleoside monophosphate biosynthetic process), GO:0009167 (purine ribonucleoside monophosphate metabolic process), GO:0009127 (purine nucleoside monophosphate biosynthetic process), GO:0009605 (response to external stimulus), GO:0006177 (GMP biosynthetic process), GO:0009168 (purine ribonucleoside monophosphate biosynthetic process), GO:0006189 ('de novo' IMP biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0030312 (external encapsulating structure), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0005618 (cell wall), GO:0009536 (plastid), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle), GO:0009505 (plant-type cell wall)