Protein: Large subunit GTPase 1 homolog (hLsg1) (EC 3.6.1.-) (Q9H089)
Organism: Homo sapiens (Human)
Function: GTPase required for the XPO1/CRM1-mediated nuclear export of the 60S ribosomal subunit. Probably acts by mediating the release of NMD3 from the 60S ribosomal subunit after export into the cytoplasm (Probable).
Subcellular location: Cytoplasm {ECO:0000269|PubMed:16209721}. Endoplasmic reticulum {ECO:0000269|PubMed:16209721}. Nucleus, Cajal body {ECO:0000269|PubMed:16209721}. Note=Shuttles between the Cajal bodies in the nucleus and the endoplasmic reticulum.
Sequence: MGRRRAPAGGSLGRALMRHQTQRSRSHRHTDSWLHTSELNDGYDWGRLNLQSVTEQSSLDDFLATAELAGTEFVAEKLNIKFVPAEARTGLLSFEESQRIKKLHEENKQFLCIPRRPNWNQNTTPEELKQAEKDNFLEWRRQLVRLEEEQKLILTPFERNLDFWRQLWRVIERSDIVVQIVDARNPLLFRCEDLECYVKEMDANKENVILINKADLLTAEQRSAWAMYFEKEDVKVIFWSALAGAIPLNGDSEEEANRDDRQSNTTKFGHSSFDQAEISHSESEHLPARDSPSLSENPTTDEDDSEYEDCPEEEEDDWQTCSEEDGPKEEDCSQDWKESSTADSEARSRKTPQKRQIHNFSHLVSKQELLELFKELHTGRKVKDGQLTVGLVGYPNVGKSSTINTIMGNKKVSVSATPGHTKHFQTLYVEPGLCLCDCPGLVMPSFVSTKAEMTCSGILPIDQMRDHVPPVSLVCQNIPRHVLEATYGINIITPREDEDPHRPPTSEELLTAYGYMRGFMTAHGQPDQPRSARYILKDYVSGKLLYCHPPPGRDPVTFQHQHQRLLENKMNSDEIKMQLGRNKKAKQIENIVDKTFFHQENVRALTKGVQAVMGYKPGSGVVTASTASSENGAGKPWKKHGNRNKKEKSRRLYKHLDM
InterPro domains: - IPR043358: Ras GTPase GNL1-like (family) [8-654]
- IPR030378: Circularly permuted (CP)-type guanine nucleotide-binding (G) domain (domain) [164-444]
- IPR006073: GTP binding domain (domain) [388-450]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [162-479]
Interaction partners: - 60S ribosomal export protein NMD3
- Eukaryotic translation initiation factor 6
- Proliferation-associated protein 2G4
- Large ribosomal subunit protein uL18
- Large ribosomal subunit protein eL27
- Cytoplasmic 60S subunit biogenesis factor ZNF622
- pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3
- Large ribosomal subunit protein uL3
- GTP-binding protein 4
- Large ribosomal subunit protein uL2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0051234 (establishment of localization), GO:0051641 (cellular localization), GO:0051640 (organelle localization), GO:0071840 (cellular component organization or biogenesis), GO:0031503 (protein-containing complex localization), GO:0032879 (regulation of localization), GO:0050794 (regulation of cellular process), GO:0051649 (establishment of localization in cell), GO:0033750 (ribosome localization), GO:0051049 (regulation of transport), GO:0044085 (cellular component biogenesis), GO:0046907 (intracellular transport), GO:0051656 (establishment of organelle localization), GO:0060341 (regulation of cellular localization), GO:0006810 (transport), GO:0000054 (ribosomal subunit export from nucleus), GO:0051169 (nuclear transport), GO:0000055 (ribosomal large subunit export from nucleus), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0032386 (regulation of intracellular transport), GO:0006913 (nucleocytoplasmic transport), GO:0042254 (ribosome biogenesis), GO:0046822 (regulation of nucleocytoplasmic transport), GO:0051168 (nuclear export)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0031974 (membrane-enclosed lumen), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005654 (nucleoplasm), GO:0031090 (organelle membrane), GO:0043233 (organelle lumen), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0012506 (vesicle membrane), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus)