Protein: GTP-binding protein GTR2 (EC 3.6.5.-) (P53290)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: GTPase involved in activation of the TORC1 signaling pathway, which promotes growth and represses autophagy in nutrient-rich conditions. Also required for TORC1 inactivation during nitrogen starvation. Required for intracellular sorting of GAP1 out of the endosome. Involved in the regulation of microautophagy.
Subcellular location: Vacuole membrane {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:15989961, ECO:0000269|PubMed:28993463}; Peripheral membrane protein {ECO:0000305}.
Sequence: MSLEATDSKAMVLLMGVRRCGKSSICKVVFHNMQPLDTLYLESTSNPSLEHFSTLIDLAVMELPGQLNYFEPSYDSERLFKSVGALVYVIDSQDEYINAITNLAMIIEYAYKVNPSINIEVLIHKVDGLSEDFKVDAQRDIMQRTGEELLELGLDGVQVSFYLTSIFDHSIYEAFSRIVQKLIPELSFLENMLDNLIQHSKIEKAFLFDVNSKIYVSTDSNPVDIQMYEVCSEFIDVTIDLFDLYKAPVLRNSQKSSDKDNVINPRNELQNVSQLANGVIIYLRQMIRGLALVAIIRPNGTDMESCLTVADYNIDIFKKGLEDIWANARASQAKNSIEDDV
InterPro domains: - IPR039400: RagC/D (family) [12-183]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [6-185]
- IPR006762: Gtr1/RagA G protein (family) [8-332]
Interaction partners: - Protein SLM4
- Protein MEH1
- GTP-binding protein GTR1
- Protein EGO2
- Target of rapamycin complex 1 subunit KOG1
- Serine/threonine-protein kinase TOR1
- Protein LTV1
- Serine/threonine-protein kinase TOR2
- Nitrogen permease regulator 3
- Restriction of telomere capping protein 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0042802 (identical protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0009605 (response to external stimulus), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0007154 (cell communication), GO:0044238 (primary metabolic process), GO:0009991 (response to extracellular stimulus), GO:1901564 (organonitrogen compound metabolic process), GO:0042594 (response to starvation), GO:0033554 (cellular response to stress), GO:0044248 (cellular catabolic process), GO:0019538 (protein metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0031668 (cellular response to extracellular stimulus), GO:0080090 (regulation of primary metabolic process), GO:1901575 (organic substance catabolic process), GO:0071496 (cellular response to external stimulus), GO:0043170 (macromolecule metabolic process), GO:0006793 (phosphorus metabolic process), GO:0036211 (protein modification process), GO:0009057 (macromolecule catabolic process), GO:0043412 (macromolecule modification), GO:0034248 (regulation of amide metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:1901565 (organonitrogen compound catabolic process), GO:0009267 (cellular response to starvation), GO:0044265 (cellular macromolecule catabolic process), GO:0009267 (cellular response to starvation), GO:0031667 (response to nutrient levels), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0031669 (cellular response to nutrient levels), GO:0010468 (regulation of gene expression), GO:0016036 (cellular response to phosphate starvation), GO:0006417 (regulation of translation), GO:0070647 (protein modification by small protein conjugation or removal), GO:0010608 (post-transcriptional regulation of gene expression), GO:0032446 (protein modification by small protein conjugation), GO:0015867 (ATP transport), GO:0006862 (nucleotide transport), GO:0016567 (protein ubiquitination), GO:0015865 (purine nucleotide transport), GO:0051503 (adenine nucleotide transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0005773 (vacuole), GO:0031090 (organelle membrane), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000323 (lytic vacuole), GO:0043231 (intracellular membrane-bounded organelle), GO:0005774 (vacuolar membrane), GO:0098588 (bounding membrane of organelle), GO:0000322 (storage vacuole), GO:0000324 (fungal-type vacuole), GO:0098852 (lytic vacuole membrane), GO:0000329 (fungal-type vacuole membrane)