Protein: Allantoate deiminase (EC 3.5.3.9) (Allantoate amidohydrolase) (AtAAH) (O49434)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Involved in the catabolism of purine nucleotides. Can use allantoate as substrate, but not Nalpha-carbamoyl-L-Asp, Nalpha-carbamoyl-L-Ala or Nalpha-carbamoyl-Gly. The sequential activity of AAH, UGLYAH and UAH allows a complete purine breakdown without the intermediate generation of urea. Involved in the regulation of seed maturation and seed dormancy.
Subcellular location: Endoplasmic reticulum {ECO:0000269|PubMed:18065556}.
Sequence: MAVPHPSSSSSRSHPFLSHVYHTSFHHHHHHNHPSLVLFWCLVFSLLSPLALSSSSSSSSSSSDSSSSSSSHISLGIGETEGTKHDLHQAILRDEAVARLHELGQVSDAATHLERTFMSPASIRAIPLIRGWMEDAGLSTWVDYMGNVHGRVEPKNGSSQALLIGSHMDTVIDAGKYDGSLGIISAISALKVLKIDGRLGELKRPVEVIAFSDEEGVRFQSTFLGSAALAGIMPVSRLEVTDKSGISVQDALKENSIDITDENLMQLKYDPASVWGYVEVHIEQGPVLEWVGYPLGVVKGIAGQTRLKVTVKGSQGHAGTVPMSMRQDPMTGAAELIVLLESVCKNPKDYLSCNVQCNEDTVESLANSLVCTVGEISTWPSASNVIPGQVTFTVDLRTIDDVGRKAILHDLSTRMYQICDKRSLLCSIERKHDADAVMSDPQLSLQLKSAAQSALKKMTGEVQDEVPVLMSGAGHDAMAMAHLTKVGMLFVRCRGGISHSPAEHVLDDDVGAAGLAILEFLESQM
InterPro domains: - IPR002933: Peptidase M20 (family) [163-516]
- IPR011650: Peptidase M20, dimerisation domain (domain) [303-416]
- IPR001261: ArgE/DapE/ACY1/CPG2/YscS, conserved site (conserved_site) [162-171]
- IPR010158: Amidase, carbamoylase-type (family) [58-525]
- IPR036264: Bacterial exopeptidase dimerisation domain (homologous_superfamily) [303-436]
Interaction partners: - Allantoinase
- (S)-ureidoglycine aminohydrolase (AtUGLYAH)
- Uricase
- Xanthine dehydrogenase 1
- AT4g00920 protein
- Wall-associated receptor kinase-like protein
- AT4g19390/T5K18_170
- NC domain-containing protein-like protein
- Ureide permease 1
- AT3g56990/F24I3_70
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016810 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds), GO:0016813 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0006807 (nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0043603 (amide metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0046483 (heterocycle metabolic process), GO:0043605 (amide catabolic process), GO:0044248 (cellular catabolic process), GO:0046700 (heterocycle catabolic process), GO:0000255 (allantoin metabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:1901361 (organic cyclic compound catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)