Protein: Aspartokinase 1, chloroplastic (EC 2.7.2.4) (Aspartate kinase 1) (Q9LYU8)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Involved in the first step of essential amino acids lysine, threonine, methionine and isoleucine synthesis via the aspartate-family pathway.
Subcellular location: Plastid, chloroplast {ECO:0000305}.
Sequence: MAATRVRCCHSNAAFTRLPLTRHRNSPTLPISLNRVDFPTLKKLSLPIGDGSSIRKVSGSGSRNIVRAVLEEKKTEAITEVDEKGITCVMKFGGSSVASAERMKEVADLILTFPEESPVIVLSAMGKTTNNLLLAGEKAVSCGVSNASEIEELSIIKELHIRTVKELNIDPSVILTYLEELEQLLKGIAMMKELTLRTRDYLVSFGECLSTRIFAAYLNTIGVKARQYDAFEIGFITTDDFTNGDILEATYPAVAKRLYDDWMHDPAVPIVTGFLGKGWKTGAVTTLGRGGSDLTATTIGKALGLKEIQVWKDVDGVLTCDPTIYKRATPVPYLTFDEAAELAYFGAQVLHPQSMRPAREGEIPVRVKNSYNPKAPGTIITKTRDMTKSILTSIVLKRNVTMLDIASTRMLGQVGFLAKVFSIFEELGISVDVVATSEVSISLTLDPSKLWSRELIQQELDHVVEELEKIAVVNLLKGRAIISLIGNVQHSSLILERAFHVLYTKGVNVQMISQGASKVNISFIVNEAEAEGCVQALHKSFFESGDLSELLIQPRLGNGSPVRTLQVEN
InterPro domains: - IPR001048: Aspartate/glutamate/uridylate kinase (domain) [87-369]
- IPR002912: ACT domain (domain) [405-483]
- IPR042199: Aspartokinase/Bifunctional aspartokinase/homoserine dehydrogenase, catalytic domain (homologous_superfamily) [126-222]
- IPR036393: Acetylglutamate kinase-like superfamily (homologous_superfamily) [89-382]
- IPR054352: Aspartokinase, ACT domain (domain) [482-541]
- IPR041746: Lysine-sensitive aspartokinase catalytic domain (domain) [87-381]
- IPR045865: ACT-like domain (homologous_superfamily) [390-545]
- IPR047896: Aspartokinase 1, ACT domain 1 (domain) [400-477]
- IPR047895: Aspartokinase 1, ACT domain 2 (domain) [479-543]
- IPR018042: Aspartate kinase, conserved site (conserved_site) [89-97]
- IPR001341: Aspartate kinase (family) [88-543]
Interaction partners: - aspartate-semialdehyde dehydrogenase
- Homoserine dehydrogenase
- Homoserine kinase
- Argininosuccinate synthase, chloroplastic
- Diaminopimelate epimerase, chloroplastic
- Asparagine synthetase 1
- 4-hydroxy-tetrahydrodipicolinate synthase 2, chloroplastic
- Cystathionine gamma-synthase 1, chloroplastic
- Probable cystathionine gamma-synthase 2
- Asparagine synthetase 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016301 (kinase activity), GO:0016774 (phosphotransferase activity, carboxyl group as acceptor), GO:0019202 (amino acid kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0009064 (glutamine family amino acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0006591 (ornithine metabolic process), GO:0009084 (glutamine family amino acid biosynthetic process), GO:0006525 (arginine metabolic process), GO:0006526 (arginine biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle)