Protein: Bifunctional pinoresinol-lariciresinol reductase (PLR-Fi1) (PLR3) ((+)-lariciresinol reductase) (EC 1.23.1.2) ((+)-pinoresinol reductase) (EC 1.23.1.1) (P93143)
Organism: Forsythia intermedia (Border forsythia) (Forsythia suspensa x Forsythia viridissima)
Function: Reductase involved in lignan biosynthesis. Catalyzes the enantioselective sequential conversion of (+)-pinoresinol into (+)-lariciresinol and of (+)-lariciresinol into (-)-secoisolariciresinol. Abstracts the 4R-hydride from the NADPH cofactor during catalysis.
Sequence: MGKSKVLIIGGTGYLGRRLVKASLAQGHETYILHRPEIGVDIDKVEMLISFKMQGAHLVSGSFKDFNSLVEAVKLVDVVISAISGVHIRSHQILLQLKLVEAIKEAGNVKRFLPSEFGMDPAKFMDTAMEPGKVTLDEKMVVRKAIEKAGIPFTYVSANCFAGYFLGGLCQFGKILPSRDFVIIHGDGNKKAIYNNEDDIATYAIKTINDPRTLNKTIYISPPKNILSQREVVQTWEKLIGKELQKITLSKEDFLASVKELEYAQQVGLSHYHDVNYQGCLTSFEIGDEEEASKLYPEVKYTSVEEYLKRYV
InterPro domains: - IPR036291: NAD(P)-binding domain superfamily (homologous_superfamily) [3-261]
- IPR050608: NmrA-type oxidoreductase, Isoflavone reductase subfamily (family) [1-312]
- IPR008030: NmrA-like domain (domain) [3-308]
- IPR045312: Phenylcoumaran benzylic ether reductase-like (domain) [5-307]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016627 (oxidoreductase activity, acting on the CH-CH group of donors), GO:0016628 (oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044282 (small molecule catabolic process), GO:0046483 (heterocycle metabolic process), GO:0044248 (cellular catabolic process), GO:0006066 (alcohol metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0018904 (ether metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0009698 (phenylpropanoid metabolic process), GO:1901575 (organic substance catabolic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0019439 (aromatic compound catabolic process), GO:0046700 (heterocycle catabolic process), GO:0034308 (primary alcohol metabolic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:0046165 (alcohol biosynthetic process), GO:0042537 (benzene-containing compound metabolic process), GO:0018958 (phenol-containing compound metabolic process), GO:0046271 (phenylpropanoid catabolic process), GO:1901503 (ether biosynthetic process), GO:1901616 (organic hydroxy compound catabolic process), GO:0046164 (alcohol catabolic process), GO:0019751 (polyol metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:1901361 (organic cyclic compound catabolic process), GO:0009806 (lignan metabolic process), GO:0009699 (phenylpropanoid biosynthetic process), GO:0009807 (lignan biosynthetic process), GO:0034309 (primary alcohol biosynthetic process), GO:0019336 (phenol-containing compound catabolic process), GO:0034311 (diol metabolic process), GO:0046173 (polyol biosynthetic process), GO:0046189 (phenol-containing compound biosynthetic process), GO:0034310 (primary alcohol catabolic process), GO:0034312 (diol biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)