Protein: Glutamate--cysteine ligase (EC 6.3.2.2) (Gamma-ECS) (GCS) (Gamma-glutamylcysteine synthetase) (Q9W3K5)
Organism: Drosophila melanogaster (Fruit fly)
Function: Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis.
Sequence: MGLLSEGSPLSWEETKALADHVREHGVNQFINLYHRLKDRQGDILKWGDEVEYIIVKFDDEQKVARVALRAQDLLAQLNEKELADPNGVKSLWRPEYGAYMIEGTPGKPFGGLMAHFNLVEANMRYRREEVTELLAKDECVMSITNFPRLGAPNFTYPLAQPRPEDPLSSARSLYFPDEAIFPGHPRFKTLTRNIRKRRGEKVSIKLKVFKDTKTKLPVEGAPPGEPDVVLLDAMGFGMGCCCLQLTFQACNITEARRLYDQLAPLCPIMLALTAASPIYRGYLTESDCRWNVISSSVDCRTEEERGLAPLDQQKFRIAKSRYDSIDSYLSPEGAKYNDVPLTYDEKVYQRLVEGGIDHLLAQHVAHLFIRDTVSLFSEKVHQNDNEDTDHFENIQSTNWQTMRFKPPPPNSSIGWRVEFRPCEAQISDFENAAIVCFVVLLTRVILSYQLNFLTPISKVDENMQTAQKRDACRKEKFWFRKSSKTTEQRAAKAQAQAQAQAKAQAQTNGKATLNGNGLANGNGNGSENSDQEEQQPLTNGSAKMNGHGSGTTNGTNGSSNGSSNGTDSDHTDTDDEENELFQLLSINEIFNGKPNVFPGLVPLIRSYLQSMEVDTDTHCTIEQYLRFIQKRAAGELITTATWMREQVLSHPDYKQDSVVSERINYDLLKRIQGIQEGKQVEPALLGQDYHSKTKTKDFIPPALQKQLAKNGCCEEK
InterPro domains: - IPR014746: Glutamine synthetase/guanido kinase, catalytic domain (homologous_superfamily) [39-505]
- IPR004308: Glutamate-cysteine ligase catalytic subunit (family) [1-695]
Interaction partners: - GCS light chain
- FI11917p
- Gamma-glutamyl transpeptidase, isoform A
- RE13973p
- Uncharacterized protein, isoform C
- Aminopeptidase
- Aminopeptidase
- Aminopeptidase
- Aminopeptidase
- Aminopeptidase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0016879 (ligase activity, forming carbon-nitrogen bonds), GO:0016881 (acid-amino acid ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009628 (response to abiotic stimulus), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0006807 (nitrogen compound metabolic process), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0001101 (response to acid chemical), GO:0006749 (glutathione metabolic process), GO:0009414 (response to water deprivation), GO:0010035 (response to inorganic substance), GO:0009415 (response to water), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006979 (response to oxidative stress), GO:0044249 (cellular biosynthetic process), GO:1901700 (response to oxygen-containing compound), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0010033 (response to organic substance), GO:1902074 (response to salt), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006750 (glutathione biosynthetic process), GO:0006518 (peptide metabolic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0043043 (peptide biosynthetic process), GO:0019184 (nonribosomal peptide biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005829 (cytosol), GO:1902494 (catalytic complex), GO:0005622 (intracellular anatomical structure), GO:0005737 (cytoplasm)