Protein: Branched-chain-amino-acid aminotransferase 2, chloroplastic (Atbcat-2) (EC 2.6.1.42) (Q9M439)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Converts 2-oxo acids to branched-chain amino acids. Shows activity with L-Leu, L-Ile and L-Val as amino donors and 2-oxoglutarate as an amino acceptor, but no activity for D-isomers of Leu, Ile, Val, Asp, Glu or Ala.
Subcellular location: Plastid, chloroplast.
Sequence: MIKTITSLRKTLVLPLHLHIRTLQTFAKYNAQAASALREERKKPLYQNGDDVYADLDWDNLGFGLNPADYMYVMKCSKDGEFTQGELSPYGNIQLSPSAGVLNYGQAIYEGTKAYRKENGKLLLFRPDHNAIRMKLGAERMLMPSPSVDQFVNAVKQTALANKRWVPPAGKGTLYIRPLLMGSGPILGLGPAPEYTFIVYASPVGNYFKEGMAALNLYVEEEYVRAAPGGAGGVKSITNYAPVLKALSRAKSRGFSDVLYLDSVKKKYLEEASSCNVFVVKGRTISTPATNGTILEGITRKSVMEIASDQGYQVVEKAVHVDEVMDADEVFCTGTAVVVAPVGTITYQEKRVEYKTGDESVCQKLRSVLVGIQTGLIEDNKGWVTDIN
InterPro domains: - IPR001544: Aminotransferase class IV (family) [108-344]
- IPR018300: Aminotransferase, class IV, conserved site (conserved_site) [271-300]
- IPR005786: Branched-chain amino acid aminotransferase II (family) [36-388]
- IPR043131: Branched-chain-amino-acid aminotransferase-like, N-terminal (homologous_superfamily) [31-215]
- IPR043132: Branched-chain-amino-acid aminotransferase-like, C-terminal (homologous_superfamily) [216-386]
- IPR036038: Aminotransferase-like, PLP-dependent enzymes (homologous_superfamily) [50-384]
- IPR033939: Branched-chain aminotransferase (family) [94-375]
Interaction partners: - Dihydroxy-acid dehydratase, chloroplastic
- Alanine--glyoxylate aminotransferase 2 homolog 2, mitochondrial
- 2-isopropylmalate synthase 2, chloroplastic
- 2-isopropylmalate synthase 1, chloroplastic
- Threonine dehydratase biosynthetic, chloroplastic
- Alanine--glyoxylate aminotransferase 2 homolog 1, mitochondrial
- 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial
- 3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial
- 2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial
- Cystathionine gamma-synthase 1, chloroplastic
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016769 (transferase activity, transferring nitrogenous groups), GO:0008483 (transaminase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044282 (small molecule catabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0044248 (cellular catabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:1901575 (organic substance catabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0016054 (organic acid catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0043436 (oxoacid metabolic process), GO:0016053 (organic acid biosynthetic process), GO:0009063 (amino acid catabolic process), GO:0009081 (branched-chain amino acid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0006551 (leucine metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0006549 (isoleucine metabolic process), GO:0009082 (branched-chain amino acid biosynthetic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:1901606 (alpha-amino acid catabolic process), GO:0046395 (carboxylic acid catabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0009083 (branched-chain amino acid catabolic process), GO:0009098 (leucine biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)