Protein: Periplasmic alpha-amylase (EC 3.2.1.1) (1,4-alpha-D-glucan glucanohydrolase) (P25718)
Organism: Escherichia coli (strain K12)
Function: Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded by the periplasmic alpha-amylase, the MalS protein.
Subcellular location: Periplasm.
Sequence: MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPTLMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIMLQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYLSGDEVKKSLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARHPAIGAGKQTTLLLKQGYGFVREHGDDKVLVVWAGQQ
InterPro domains: - IPR017853: Glycoside hydrolase superfamily (homologous_superfamily) [179-643]
- IPR006047: Glycosyl hydrolase family 13, catalytic domain (domain) [193-637]
- IPR014635: Alpha-amylase, MalS type (family) [1-676]
Interaction partners: - 4-alpha-glucanotransferase
- Glycogen debranching enzyme
- 1,4-alpha-glucan branching enzyme GlgB
- Cytoplasmic alpha-amylase
- Maltodextrin phosphorylase
- Glycogen phosphorylase
- Maltodextrin glucosidase
- Maltose/maltodextrin-binding periplasmic protein
- Maltose/maltodextrin transport system permease protein MalF
- Maltose/maltodextrin transport system permease protein MalG
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016798 (hydrolase activity, acting on glycosyl bonds), GO:0004553 (hydrolase activity, hydrolyzing O-glycosyl compounds), GO:0016160 (amylase activity), GO:0004556 (alpha-amylase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0005975 (carbohydrate metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0044248 (cellular catabolic process), GO:0044275 (cellular carbohydrate catabolic process), GO:0009057 (macromolecule catabolic process), GO:0016052 (carbohydrate catabolic process), GO:0044264 (cellular polysaccharide metabolic process), GO:0005976 (polysaccharide metabolic process), GO:0000272 (polysaccharide catabolic process), GO:0044042 (glucan metabolic process), GO:0044247 (cellular polysaccharide catabolic process), GO:0006073 (cellular glucan metabolic process), GO:0005983 (starch catabolic process), GO:0009251 (glucan catabolic process), GO:0005982 (starch metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0031975 (envelope), GO:0042597 (periplasmic space), GO:0030313 (cell envelope), GO:0030288 (outer membrane-bounded periplasmic space)