Protein: 4-coumarate--CoA ligase-like 9 (EC 6.2.1.-) (4-coumarate--CoA ligase isoform 4) (At4CL4) (Q84P23)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Contributes to jasmonic acid biosynthesis by initiating the beta-oxidative chain shortening of its precursors. Converts 12-oxo-phytodienoic acid (OPDA) into OPDA-CoA. Follows a two-step reaction mechanism, wherein the carboxylate substrate first undergoes adenylation by ATP, followed by a thioesterification in the presence of CoA to yield the final CoA thioester (By similarity).
Subcellular location: Peroxisome {ECO:0000269|PubMed:15677481}.
Sequence: MLTKTNDSRLIDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEALTYLVNSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALMSIGVVVSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSWLNRSDSSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQDPVNYDRVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVTGMPVSPPLIVALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLTESSGPAASTFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGYVGNEKASAETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSNPDVIDAAVVPFPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYKKVRRVAFINAIPKNPAGKILRRELTKIAVDGNASKL
InterPro domains: - IPR042099: ANL, N-terminal domain (homologous_superfamily) [53-455]
- IPR000873: AMP-dependent synthetase/ligase domain (domain) [64-417]
- IPR045851: AMP-binding enzyme, C-terminal domain superfamily (homologous_superfamily) [456-557]
- IPR020845: AMP-binding, conserved site (conserved_site) [209-220]
- IPR025110: AMP-binding enzyme, C-terminal domain (domain) [469-544]
Interaction partners: - Trans-cinnamate 4-monooxygenase
- Cinnamoyl-CoA reductase 2
- Cinnamoyl-CoA reductase 1
- Cytochrome P450 84A1
- F5F19.5 protein
- Flavone 3'-O-methyltransferase 1
- Phenylalanine ammonia-lyase 2
- Phenylalanine ammonia-lyase 3
- Phenylalanine ammonia-lyase 1
- Phenylalanine ammonia-lyase 4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0016877 (ligase activity, forming carbon-sulfur bonds), GO:0016405 (CoA-ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0042180 (cellular ketone metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:1901661 (quinone metabolic process), GO:0042181 (ketone biosynthetic process), GO:0006743 (ubiquinone metabolic process), GO:1901663 (quinone biosynthetic process), GO:0006744 (ubiquinone biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0042579 (microbody), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005777 (peroxisome)