Protein: Equilibrative nucleoside transporter 3 (Q99P65)
Organism: Mus musculus (Mouse)
Function: Uniporter that mediates the facilitative transport of nucleoside across lysosomal and mitochondrial membranes. Functions as a non-electrogenic Na(+)-independent transporter. Substrate transport is pH-dependent and enhanced under acidic condition, probably reflecting the location of the transporter in acidic intracellular compartments. Proton is not a cotransporting ion but most likely change the ionization state of the transporter which dictates transport-permissible/impermissible conformation for nucleoside translocation (By similarity). May direct the nucleoside transport from lysosomes to cytosol or cytosol to mitochondria to facilitate the fundamental function of salvage synthesis of nucleic acids. Involved in the transport of nucleosides (adenosine, guanosine, uridine, thymidine, cytidine and inosine) and deoxynucleosides (deoxyadenosine, deoxycytidine). Also mediates transport of purine nucleobases (adenine, guanine), and pyrimidine nucleobases (uracil). Also able to transport monoamine neurotransmitters dopamine, serotonin, noradrenaline and tyramine (By similarity). Capable of transporting ATP (By similarity). Mediates nucleoside export from lysosomes in macrophages, which regulates macrophage functions and numbers
Subcellular location: Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
Sequence: MAFASEDNVYHSSNAVYRAPSNHQEADQEALLGKLLDYPAPGLQRPEDRFNGAYIIFFCLGIGGLLPWNFFVTAKEYWAYKLRNCSSPASGEDPEDMDILNYFESYLAVASTVPSLLFLVANFLLVNRVQVHVRVLASLSVSLAIFVVMIVLVKVDTSSWTRGFFSLTIACMAIISSSSTIFNSSVYGLTGSFPMRNAQALISGGAMGGTVSAVALLVDLAASSDVRDSTLAFFLMAAVFLGLCMGLYLLLSQLEYARYYMRPVAPVRVFSGEDNPSQDAPSASSVAPASRVMHTPPLGPILKKTASLGFCAVSLYFVTAFIIPAISTNIQSMHKGTGSPWTSKFFVPLTVFLLFNFADLCGRQVTAWIQVPGPRSKLLPGLVVSRFCLVPLFLLCNYQPRSHLTKVLFQSDIYPVLFTCLLGLSNGYLSTLVLIYGPKIVPRELAEATSVVMLFYMSVGLMLGSACAALLEHFI
InterPro domains: - IPR002259: Equilibrative nucleoside transporter (family) [29-473]
Interaction partners: - Equilibrative nucleoside transporter 2
- Equilibrative nucleoside transporter 1
- Solute carrier family 28 member 3
- Sodium/nucleoside cotransporter 1
- Sodium/nucleoside cotransporter 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity), GO:0015932 (nucleobase-containing compound transmembrane transporter activity), GO:1901505 (carbohydrate derivative transmembrane transporter activity), GO:0005337 (nucleoside transmembrane transporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0065008 (regulation of biological quality), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0042221 (response to chemical), GO:0055085 (transmembrane transport), GO:0051234 (establishment of localization), GO:0051716 (cellular response to stimulus), GO:0003008 (system process), GO:0009887 (animal organ morphogenesis), GO:0060322 (head development), GO:0070887 (cellular response to chemical stimulus), GO:0048731 (system development), GO:0006810 (transport), GO:0048513 (animal organ development), GO:0048513 (animal organ development), GO:0007589 (body fluid secretion), GO:0071495 (cellular response to endogenous stimulus), GO:1901700 (response to oxygen-containing compound), GO:0003013 (circulatory system process), GO:0010033 (response to organic substance), GO:0071417 (cellular response to organonitrogen compound), GO:0003018 (vascular process in circulatory system), GO:0007420 (brain development), GO:0006836 (neurotransmitter transport), GO:0001504 (neurotransmitter uptake), GO:0098657 (import into cell), GO:0071705 (nitrogen compound transport), GO:0007399 (nervous system development), GO:0008015 (blood circulation), GO:0071310 (cellular response to organic substance), GO:0048732 (gland development), GO:1901699 (cellular response to nitrogen compound), GO:0071702 (organic substance transport), GO:0007417 (central nervous system development), GO:0046903 (secretion), GO:1901701 (cellular response to oxygen-containing compound), GO:0015931 (nucleobase-containing compound transport), GO:0030879 (mammary gland development), GO:1901264 (carbohydrate derivative transport), GO:0007595 (lactation), GO:1901264 (carbohydrate derivative transport), GO:0015858 (nucleoside transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0031090 (organelle membrane), GO:0031967 (organelle envelope), GO:0005739 (mitochondrion), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0005740 (mitochondrial envelope), GO:0019866 (organelle inner membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0031966 (mitochondrial membrane), GO:0005743 (mitochondrial inner membrane)