Protein: DASH complex subunit SPC34 (34 kDa spindle pole component protein) (Outer kinetochore protein SPC34) (P36131)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids.
Subcellular location: Nucleus {ECO:0000269|PubMed:14562095}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:11782438, ECO:0000269|PubMed:14562095}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:11782438, ECO:0000269|PubMed:14562095}. Chromosome {ECO:0000269|PubMed:36701236}. Note=Associates with the mitotic spindle and the kinetochore (PubMed:14562095). Associates with origin of replication (ORI) sites during interphase (PubMed:36701236). {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:36701236}.
Sequence: MGESLDRCIDDINRAVDSMSTLYFKPPGIFHNAILQGASNKASIRKDITRLIKDCNHDEAYLLFKVNPEKQSVSRRDGKEGVFDYVIKRDTDMKRNRRLGRPGEKPIIHVPKEVYLNKDRLDLNNKRRRTATTSGGGLNGFIFDTDLIGSSVISNSSSGTFKALSAVFKDDPQIQRLLYALENGSVLMEEESNNQRRKTIFVEDFPTDLILKVMAEVTDLWPLTEFKQDYDQLYHNYEQLSSKLRFIKKEVLLQDDRLKTMSQYHPSSSHDVAKIIRKEKDEIRRLEMEIANLQE
InterPro domains: - IPR013966: DASH complex subunit Spc34 (family) [5-292]
Interaction partners: - DASH complex subunit DAD4
- DASH complex subunit DAD2
- DASH complex subunit ASK1
- DASH complex subunit DUO1
- DASH complex subunit DAM1
- DASH complex subunit DAD3
- DASH complex subunit DAD1
- DASH complex subunit SPC19
- DASH complex subunit HSK3
- Probable electron transfer flavoprotein subunit alpha, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0009058 (biosynthetic process), GO:0019222 (regulation of metabolic process), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0033036 (macromolecule localization), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:1901576 (organic substance biosynthetic process), GO:0044085 (cellular component biogenesis), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0051649 (establishment of localization in cell), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0051236 (establishment of RNA localization), GO:0043170 (macromolecule metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:1901360 (organic cyclic compound metabolic process), GO:0046907 (intracellular transport), GO:0006139 (nucleobase-containing compound metabolic process), GO:0006403 (RNA localization), GO:0031325 (positive regulation of cellular metabolic process), GO:0006810 (transport), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0050658 (RNA transport), GO:1901362 (organic cyclic compound biosynthetic process), GO:0071705 (nitrogen compound transport), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051052 (regulation of DNA metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0140014 (mitotic nuclear division), GO:0071702 (organic substance transport), GO:0051169 (nuclear transport), GO:0010468 (regulation of gene expression), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0022607 (cellular component assembly), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0006259 (DNA metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0043933 (protein-containing complex organization), GO:0008104 (protein localization), GO:0006996 (organelle organization), GO:0010467 (gene expression), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0070925 (organelle assembly), GO:0006913 (nucleocytoplasmic transport), GO:0033365 (protein localization to organelle), GO:0016070 (RNA metabolic process), GO:0065003 (protein-containing complex assembly), GO:0071824 (protein-DNA complex subunit organization), GO:2001141 (regulation of RNA biosynthetic process), GO:0006310 (DNA recombination), GO:0006355 (regulation of DNA-templated transcription), GO:0006405 (RNA export from nucleus), GO:0051276 (chromosome organization), GO:0015931 (nucleobase-containing compound transport), GO:1902680 (positive regulation of RNA biosynthetic process), GO:0051028 (mRNA transport), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0034504 (protein localization to nucleus), GO:0051170 (import into nucleus), GO:0045893 (positive regulation of DNA-templated transcription), GO:0050657 (nucleic acid transport), GO:0006352 (DNA-templated transcription initiation), GO:0071103 (DNA conformation change), GO:0032200 (telomere organization), GO:0016973 (poly(A)+ mRNA export from nucleus), GO:2000045 (regulation of G1/S transition of mitotic cell cycle), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:0051168 (nuclear export), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0065004 (protein-DNA complex assembly), GO:0006351 (DNA-templated transcription), GO:0045944 (positive regulation of transcription by RNA polymerase II), GO:0006367 (transcription initiation at RNA polymerase II promoter), GO:0018394 (peptidyl-lysine acetylation), GO:0070897 (transcription preinitiation complex assembly), GO:0006366 (transcription by RNA polymerase II), GO:0018393 (internal peptidyl-lysine acetylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140513 (nuclear protein-containing complex), GO:0005667 (transcription regulator complex), GO:0090575 (RNA polymerase II transcription regulator complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070847 (core mediator complex), GO:0005634 (nucleus)