Protein: Double-strand-specific pac1 ribonuclease (EC 3.1.26.3) (Protein hcs) (P22192)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Digests double-stranded RNA. Converts long double-stranded RNAs into short oligonucleotides, leaving 5'-phosphates on their cleavage products. Probably inhibits mating and meiosis by degrading a specific mRNA required for sexual development.
Sequence: MGRFKRHHEGDSDSSSSASDSLSRGRRSLGHKRSSHIKNRQYYILEKKIRKLMFAMKALLEETKHSTKDDVNLVIPGSTWSHIEGVYEMLKSRHDRQNEPVIEEPSSHPKNQKNQENNEPTSEEFEEGEYPPPLPPLRSEKLKEQVFMHISRAYEIYPNQSNPNELLDIHNERLEFLGDSFFNLFTTRIIFSKFPQMDEGSLSKLRAKFVGNESADKFARLYGFDKTLVLSYSAEKDQLRKSQKVIADTFEAYLGALILDGQEETAFQWVSRLLQPKIANITVQRPIDKLAKSKLFHKYSTLGHIEYRWVDGAGGSAEGYVIACIFNGKEVARAWGANQKDAGSRAAMQALEVLAKDYSKFAR
InterPro domains: - IPR044449: Rnt1/Pac1, double-stranded RNA binding domain, fungi (domain) [288-355]
- IPR014720: Double-stranded RNA-binding domain (domain) [285-356]
- IPR036389: Ribonuclease III, endonuclease domain superfamily (homologous_superfamily) [129-284]
- IPR000999: Ribonuclease III domain (domain) [139-283]
Interaction partners: - Small ribosomal subunit protein uS9m
- Protein argonaute
- Large ribosomal subunit protein bL27m
- Large ribosomal subunit protein bL17m
- Large ribosomal subunit protein bL19m
- Small ribosomal subunit protein bS6m
- Large ribosomal subunit protein mL46
- Large ribosomal subunit protein bL32m
- Uncharacterized protein C328.01c
- Large ribosomal subunit protein bL33m
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0005515 (protein binding), GO:0140098 (catalytic activity, acting on RNA), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0004518 (nuclease activity), GO:0004540 (ribonuclease activity), GO:0004521 (RNA endonuclease activity), GO:0004519 (endonuclease activity), GO:0016891 (RNA endonuclease activity, producing 5'-phosphomonoesters), GO:0016893 (endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0009892 (negative regulation of metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0043933 (protein-containing complex organization), GO:0010467 (gene expression), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression), GO:0022607 (cellular component assembly), GO:0016070 (RNA metabolic process), GO:0022618 (ribonucleoprotein complex assembly), GO:0065003 (protein-containing complex assembly), GO:0031047 (RNA-mediated gene silencing), GO:0006396 (RNA processing), GO:0071826 (ribonucleoprotein complex subunit organization), GO:0034470 (ncRNA processing), GO:0070918 (regulatory ncRNA processing), GO:0034660 (ncRNA metabolic process), GO:0035196 (miRNA processing), GO:0030422 (siRNA processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus)