Protein: Probable RNA helicase armi (EC 3.6.4.13) (Protein armitage) (Q6J5K9)
Organism: Drosophila melanogaster (Fruit fly)
Function: Probable RNA helicase required for axial polarization of the oocyte during early and mid oogenesis. Plays a central role in RNA interference (RNAi) process, a process that mediates mRNA destruction of translational repression. Required for the assembly of the RISC complex, a complex required for target RNA destruction or repression. May be required in the RISC assembly to unwind miRNAs, in the production of single-stranded miRNA from the double-stranded miRNA, a key step in RISC formation. Required both for the translational control of oskar (osk) mRNA and cytoskeletal polarization in the oocyte. Required for somatic primary piRNA biogenesis.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:15035984, ECO:0000269|PubMed:20966047}. Note=Component of Yb bodies, an electron dense structure containing Yb protein found in somatic cells of ovary and testis.
Sequence: MFTYVSKFFTNPDRNREDILESLDRENSFLDQKLMEEKMDQQLKANPNEINGVLSNKIAELTHGLSEMDVSKESSCTARKGVITSLDGDRGVIDKDVLFETKVAEDIILDLHVGCVVEYLTFTTGEAMRVVKVKSILEHSWEDTSQKEIEKAVDNLKNEKPTFFNTETRSVLGLISQRLASSIDVETEYGQLTVELDNIEMNFIPTNGDRVRLECNIQLDDGFVDKQGEILEVTKLFPTRIQEGEKCIVERVYVHMVVLGPETYILKTDLPTGTDLHLGDIVLADLIECQYSKFTRRAIKITPLEKNFGATKLTQQSSMGSSGSGKAVTVTGVNRFITAELWQKESVSLKLTNNLNRTLRLESITVCNDSESQLSVVSPLESKEISSGSEITVTFEIHTQFLGEAIEKYVLNFDLLKVRRVFTVIVCKTKEEVAEAEKRMIAAEALMAPGRNSQERSRFYANQVWCNKVDVIPGQQIVTKRRFVALRLGCFEVPKELRQICLTSERRQEMIKAIEQHYSFLTEPLSIKTYMHRFRLLLHLEEIECFVNFRNYDRDRAHFLRDGEFLTLQIENLAERRPSLVIGDTLRVINPWSDPDSQTTKSYEGIIHKVLFDRILLKFHSSFQEKYNGEDYRLEFYFSRYSFRKQHHAISKIVGVMGEDFLFPSKVTKRENPQLDVYMKDDDMYLYDSKLEWYNQSLNSIQKRAVFNILRGEAENIPYVLFGPPGSGKTMTLIETLLQLVRNLPGARILVGTPSNSSADLVTKRLIDSKALLQGDFIRLVSYNQVEKDLIPPEIMSYCATSDVGAVGSCEDKMMVTESGLKLRCQAKFIGTHRITISTCTTLGNFLQLGFPAGHFTHVLFDEAGQCTEPETMVPIVMLTKKRSQVVLSGDPRQLQSIVTSRIASKMGFSISFLERLLERSPYRKDLQRFPESSGYNPLVLTKLLYNYRALPSIMSIYSRLFYDDELIPVLSEKDSRESRLLSKLRCVFESEKDIPQAHGTFFYGIIGENRQNNDSPSWFNPQEVREVFLMTIALYRANVTADQIGIITPYQKQVKMLRSMFIGTDVVMPKIGSVEEFQGQERDIILISTVRSSEEILRMDARFSLGFVRCSKRLNVAVSRARAMMIIFGNPHLLAVDECWRQLILFCVKNNAYFGCDLPQMVINQKDEVPVCLETFVPSLNTTDDLN
InterPro domains: - IPR041677: DNA2/NAM7 helicase, helicase domain (domain) [698-901]
- IPR047187: Upf1-like, C-terminal helicase domain (domain) [952-1148]
- IPR049080: Helicase MOV-10-like, beta-barrel domain (domain) [559-636]
- IPR041679: DNA2/NAM7 helicase-like, C-terminal (domain) [940-1132]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [638-1165]
- IPR049079: Helicase MOV-10, helical domain (domain) [488-544]
Interaction partners: - Protein piwi
- Protein vreteno
- RNA helicase
- Mitochondrial cardiolipin hydrolase
- GH09064p
- Putative ATP-dependent RNA helicase SoYb
- Inactive peptidyl-prolyl cis-trans isomerase shutdown
- Probable ATP-dependent RNA helicase spindle-E
- Protein aubergine
- Tudor domain-containing protein krimp
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0009892 (negative regulation of metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0044248 (cellular catabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0034655 (nucleobase-containing compound catabolic process), GO:0009057 (macromolecule catabolic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0090304 (nucleic acid metabolic process), GO:0044265 (cellular macromolecule catabolic process), GO:0019439 (aromatic compound catabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0046700 (heterocycle catabolic process), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression), GO:0016070 (RNA metabolic process), GO:0006402 (mRNA catabolic process), GO:0006401 (RNA catabolic process), GO:0016071 (mRNA metabolic process), GO:0000956 (nuclear-transcribed mRNA catabolic process), GO:0000184 (nuclear-transcribed mRNA catabolic process, nonsense-mediated decay)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)