Protein: Cytoplasmic tRNA 2-thiolation protein 2 (O65628)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6/CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03054}.
Sequence: MACNSSGCESGCYDREKDNGSKIVDDAVSGGGNHESVCVKCKCNAPMTFGDGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQFVHELQIKALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSPPAKDLHVVPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYNRLVLGSCTSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLLDGLKTVELACRSQCGINDLVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDSNVPMATQRRLKRFNLKYDGSMTTEAFCPICNGPLNRSDSSELDTFEEGQESDVLYAACCSSCRFQILPQDGSSLEQFSSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLDDEEVV
InterPro domains: - IPR014729: Rossmann-like alpha/beta/alpha sandwich fold (homologous_superfamily) [37-394]
- IPR019407: Cytoplasmic tRNA 2-thiolation protein 2 (family) [33-447]
Interaction partners: - Cytoplasmic tRNA 2-thiolation protein 1
- 2-thiocytidine tRNA biosynthesis protein, TtcA
- tRNA
- Adenylyltransferase and sulfurtransferase MOCS3
- Nucleobase-ascorbate transporter 12
- Acetylornithine deacetylase
- Purine-uracil permease NCS1
- DNA replication licensing factor MCM5
- Nucleobase-ascorbate transporter 3
- Nucleobase-ascorbate transporter 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009628 (response to abiotic stimulus), GO:0044237 (cellular metabolic process), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0019222 (regulation of metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0006970 (response to osmotic stress), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0009651 (response to salt stress), GO:0051252 (regulation of RNA metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0010468 (regulation of gene expression), GO:0031326 (regulation of cellular biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)