Protein: Serine/threonine-protein kinase grp (EC 2.7.11.1) (Chk1 homolog) (Protein grapes) (O61661)
Organism: Drosophila melanogaster (Fruit fly)
Function: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. May phosphorylate the CDC25 phosphatase stg, which promotes its degradation. This results in increased inhibitory tyrosine phosphorylation of Cdk1-cyclin complexes and consequent inhibition of cell cycle progression.
Subcellular location: Nucleus {ECO:0000269|PubMed:10980701, ECO:0000269|PubMed:16079276}.
Sequence: MAATLTEAGTGPAATREFVEGWTLAQTLGEGAYGEVKLLINRQTGEAVAMKMVDLKKHPDAANSVRKEVCIQKMLQDKHILRFFGKRSQGSVEYIFLEYAAGGELFDRIEPDVGMPQHEAQRYFTQLLSGLNYLHQRGIAHRDLKPENLLLDEHDNVKISDFGMATMFRCKGKERLLDKRCGTLPYVAPEVLQKAYHAQPADLWSCGVILVTMLAGELPWDQPSTNCTEFTNWRDNDHWQLQTPWSKLDTLAISLLRKLLATSPGTRLTLEKTLDHKWCNMQFADNERSYDLVDSAAALEICSPKAKRQRLQSSAHLSNGLDDSISRNYCSQPMPTMRSDDDFNVRLGSGRSKEDGGDRQTLAQEARLSYSFSQPALLDDLLLATQMNQTQNASQNYFQRLVRRMTRFFVTTRWDDTIKRLVGTIERLGGYTCKFGDDGVVTVSTVDRNKLRLVFKAHIIEMDGKILVDCRLSKGCGLEFKRRFIKIKNALEDIVLKGPTTWPIAIATNSVP
InterPro domains: - IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [18-280]
- IPR034670: Checkpoint kinase 1, catalytic domain (domain) [20-279]
- IPR008271: Serine/threonine-protein kinase, active site (active_site) [139-151]
- IPR017441: Protein kinase, ATP binding site (binding_site) [28-51]
- IPR000719: Protein kinase domain (domain) [22-279]
Interaction partners: - Serine/threonine-protein kinase ATR
- Claspin
- DNA replication licensing factor MCM4
- Ovarian-specific serine/threonine-protein kinase Lok
- M-phase inducer phosphatase
- Cdc25-like protein phosphatase twine
- CDC45L
- DNA repair protein Rad51 homolog
- Protein phosphatase PP2A 55 kDa regulatory subunit
- Protein MCM10 homolog
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0051641 (cellular localization), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0033036 (macromolecule localization), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0019538 (protein metabolic process), GO:0070727 (cellular macromolecule localization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0006793 (phosphorus metabolic process), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0006259 (DNA metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0008104 (protein localization), GO:0033365 (protein localization to organelle), GO:0006281 (DNA repair), GO:0006468 (protein phosphorylation), GO:0034502 (protein localization to chromosome), GO:0016310 (phosphorylation), GO:0018105 (peptidyl-serine phosphorylation), GO:0018209 (peptidyl-serine modification)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000785 (chromatin), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus), GO:0005694 (chromosome)