Protein: Pectate lyase L (EC 4.2.2.2) (Q76EC9)
Organism: Thermoclostridium stercorarium (Clostridium stercorarium)
Function: Cleaves polygalacturonate or partially methylated pectin. When assayed on polygalacturonate or on pectin, it releases monogalacturonate as the principal product.
Subcellular location: Secreted {ECO:0000269|PubMed:16306696}.
Sequence: MRNCKGLSILLCFLLVFFAMPFPAVAEEAEAMPVSEASEWSFSAFGSNTSVEKNPDPMINSDGSVKIVANGGKIASNEQGISFYYREVPSDANFEIKAKAEVLNFKGDDKQVSFGLMLMDQIGQHRNSEKHNSNYIAVGALDTIIKAFYMQESLTKTDMLSQTPSQGDIFELSIKKSGDNYVLTCNGTTETFTLPGLFSDTIYVGIYAARNAEIKFSDLNFTLDTKDIVDLSVDLSGMKTSYLVDEPLNLKGLKVTAHYSDGTSEELTEEDYIVTGFDSSTPGTNTICINVGEISKTIDLEILPLTCTKLTVKYLPAKTDYYLGDSFNPEGLKVIAEYNDGYKVTELTEDKYALYIAGKAAEDYVFSKAGTQKVEVISRENPSVKTGFEVNISDASIESLEISRKPEKTAYFIGDEPDLTGLVVYARYSDGSKVRLDKSEYEVKGFDSSAPGEKEITVYHKGKTVAFSVVVKEKEVMGIEVTKYPKTTYYIGETFNAEGLEVSKVYDNGDREPLTDFSVDASAFDGSTPGVYDVIISADGFDPITLKVTVREKTEYEWKAIRFGQSTSDSKNYVNFLDNGAVEIVALEGGGKIATDHDGITFYYTEIDAKDNFVLSADIKVKEYAKNPHDGQESFGIMARDAIGTPGDSSIFASNIAAIGGFSGGTKSPNGTQLFIRTGVSSPDGAGSKGIRRIMIKDEKPGPDNTYPAAEYRLTLAKTNSGFVGKLNDGEEVIFYEPDILNVQDSKIYVGFFAARLATIEVSNIELYVSSSETDAPRYIPPEAPVTPSLQILSLDKTSNVNYSLVVKPNVNGSITVKQGAEILVRDVTVNAGEKYSVDAVLEKNSENPFTVIFIPDDTQNLSSYEKIIKNFSVTMRTYNEGGNIYVSPNGTPYGDGTKDNPLDLDTAIAFVKEGQKIILMNGVYKRDSALVISRYNDGTAENRKYLVAEPGSRPVIDFDKKGQGVTLSGNYWYIEGIDFARSAPNYPGFIIGGNYNIVENCRFYENGDTGLQISRTDSSENIAEWPSYNKIINCESFDNRDPSENNADGFAAKLTCGVGNMFIGCVSHHNIDDGWDLYTKAGTGAIGPVIIDSCIAYENGTLTDGTVGKGDKNGFKLGGEGVPVQHIIKNSIAFNNGAVGFTSNSNPSVIAINNIAYNNAKGNLVFTSYSGIETHFVVDGFVSYNTEGAPRDSATGVPASDDNYLFDGTKSVNKSGEELTEKEFIERLLELISKIKSIK
InterPro domains: - IPR052052: Polysaccharide Lyase 9 (family) [880-1177]
- IPR011050: Pectin lyase fold/virulence factor (homologous_superfamily) [883-1217]
- IPR012334: Pectin lyase fold (homologous_superfamily) [872-1229]
- IPR022038: Ig-like domain, bacterial type (domain) [238-550]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0048583 (regulation of response to stimulus), GO:0009605 (response to external stimulus), GO:0035821 (modulation of process of another organism), GO:0044403 (biological process involved in symbiotic interaction), GO:0009607 (response to biotic stimulus), GO:0051707 (response to other organism), GO:0048585 (negative regulation of response to stimulus), GO:0051701 (biological process involved in interaction with host), GO:0075136 (response to host), GO:0043207 (response to external biotic stimulus), GO:0052173 (response to defenses of other organism), GO:0080134 (regulation of response to stress), GO:0044003 (modulation by symbiont of host process), GO:0031348 (negative regulation of defense response), GO:0044414 (suppression of host defenses by symbiont), GO:0031347 (regulation of defense response), GO:0052200 (response to host defenses), GO:0052031 (modulation by symbiont of host defense response), GO:0052572 (response to host immune response), GO:0052170 (suppression by symbiont of host innate immune response), GO:0030682 (mitigation of host defenses by symbiont), GO:0052553 (modulation by symbiont of host immune response), GO:0052562 (suppression by symbiont of host immune response), GO:0052167 (modulation by symbiont of host innate immune response)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0009274 (peptidoglycan-based cell wall)