Protein: TcdA protein (O86141)
Organism: Clostridioides difficile (Peptoclostridium difficile)
Function: Not known
Subcellular location: Host cell membrane {ECO:0000256|ARBA:ARBA00004165}. Host cytoplasm, host cytosol {ECO:0000256|ARBA:ARBA00023586}. Secreted {ECO:0000256|ARBA:ARBA00004613}.
Sequence: VLISKEELIKLAYSIRPRENEYKTVLTNLDEYNKLTTNNNENKYLQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWIGGEVSDIVLEYIKQWADINAEYNIRLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEIQNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVLTIDDIIKSYLISEYNRDETLLESYRTNSLRKINSNHGIDVRANSLFTEQELLNIYNQELLNRGNLAAASDIVRLLALKNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENFDKLDQQLKDNFKLIIESKSEKCEIFSKLENLNVSDLEIKIRFALGSVINQALISKKGSYLTNLVIEQIKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIAPYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLSQLTEQEINSLWSFDQASAKYQFEKYVKDYTGGSLSEDNEVDFNKNTALDKNYLLNNKIPSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSHFLSDDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLDTIKLDISPKNVEVNLL
InterPro domains: - IPR024770: TcdA/TcdB toxin, catalytic glycosyltransferase domain (domain) [94-466]
- IPR038383: CGT/MARTX, cysteine protease (CPD) domain superfamily (homologous_superfamily) [542-697]
- IPR029044: Nucleotide-diphospho-sugar transferases (homologous_superfamily) [2-539]
- IPR024772: TcdA/TcdB toxin, N-terminal helical domain (domain) [20-81]
- IPR020974: CGT/MARTX, cysteine protease (CPD) domain (domain) [568-697]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0090729 (toxin activity), GO:0036094 (small molecule binding), GO:0046812 (host cell surface binding), GO:0016740 (transferase activity), GO:0016787 (hydrolase activity), GO:0043167 (ion binding), GO:0140096 (catalytic activity, acting on a protein), GO:0043178 (alcohol binding), GO:0016757 (glycosyltransferase activity), GO:0008233 (peptidase activity), GO:0046789 (host cell surface receptor binding), GO:0043168 (anion binding), GO:0016758 (hexosyltransferase activity), GO:0008194 (UDP-glycosyltransferase activity), GO:0046527 (glucosyltransferase activity), GO:0035251 (UDP-glucosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0023057 (negative regulation of signaling), GO:0009058 (biosynthetic process), GO:0044068 (modulation by symbiont of host cellular process), GO:0006807 (nitrogen compound metabolic process), GO:0070085 (glycosylation), GO:0071840 (cellular component organization or biogenesis), GO:0035821 (modulation of process of another organism), GO:0071704 (organic substance metabolic process), GO:0044403 (biological process involved in symbiotic interaction), GO:0034260 (negative regulation of GTPase activity), GO:0065008 (regulation of biological quality), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0023051 (regulation of signaling), GO:0065009 (regulation of molecular function), GO:0048523 (negative regulation of cellular process), GO:0048585 (negative regulation of response to stimulus), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0044501 (modulation of signal transduction in another organism), GO:0050790 (regulation of catalytic activity), GO:0009968 (negative regulation of signal transduction), GO:0010648 (negative regulation of cell communication), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044092 (negative regulation of molecular function), GO:0019538 (protein metabolic process), GO:0009966 (regulation of signal transduction), GO:0044249 (cellular biosynthetic process), GO:0051130 (positive regulation of cellular component organization), GO:0032970 (regulation of actin filament-based process), GO:0043170 (macromolecule metabolic process), GO:0043413 (macromolecule glycosylation), GO:1901135 (carbohydrate derivative metabolic process), GO:0052027 (modulation by symbiont of host signal transduction pathway), GO:0051701 (biological process involved in interaction with host), GO:0010646 (regulation of cell communication), GO:0044003 (modulation by symbiont of host process), GO:0016043 (cellular component organization), GO:0051128 (regulation of cellular component organization), GO:0044260 (cellular macromolecule metabolic process), GO:0090066 (regulation of anatomical structure size), GO:0032535 (regulation of cellular component size), GO:0009059 (macromolecule biosynthetic process), GO:0043086 (negative regulation of catalytic activity), GO:0006508 (proteolysis), GO:0051336 (regulation of hydrolase activity), GO:0043244 (regulation of protein-containing complex disassembly), GO:0051604 (protein maturation), GO:0032956 (regulation of actin cytoskeleton organization), GO:0030111 (regulation of Wnt signaling pathway), GO:0036211 (protein modification process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1902903 (regulation of supramolecular fiber organization), GO:0006486 (protein glycosylation), GO:0043412 (macromolecule modification), GO:0009100 (glycoprotein metabolic process), GO:1902905 (positive regulation of supramolecular fiber organization), GO:0033043 (regulation of organelle organization), GO:0043243 (positive regulation of protein-containing complex disassembly), GO:0030178 (negative regulation of Wnt signaling pathway), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0010638 (positive regulation of organelle organization), GO:0010467 (gene expression), GO:1902531 (regulation of intracellular signal transduction), GO:0051493 (regulation of cytoskeleton organization), GO:1901881 (positive regulation of protein depolymerization), GO:0051346 (negative regulation of hydrolase activity), GO:1901879 (regulation of protein depolymerization), GO:0016485 (protein processing), GO:0030832 (regulation of actin filament length), GO:0006493 (protein O-linked glycosylation), GO:0018193 (peptidyl-amino acid modification), GO:0043087 (regulation of GTPase activity), GO:0051056 (regulation of small GTPase mediated signal transduction), GO:0030836 (positive regulation of actin filament depolymerization), GO:0110053 (regulation of actin filament organization), GO:0009101 (glycoprotein biosynthetic process), GO:0051495 (positive regulation of cytoskeleton organization), GO:0008064 (regulation of actin polymerization or depolymerization), GO:0046578 (regulation of Ras protein signal transduction), GO:0030834 (regulation of actin filament depolymerization), GO:0018210 (peptidyl-threonine modification), GO:0016540 (protein autoprocessing), GO:0035023 (regulation of Rho protein signal transduction)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0018995 (host cellular component), GO:0005576 (extracellular region), GO:0033643 (host cell part), GO:0043657 (host cell), GO:0033644 (host cell membrane), GO:0033646 (host intracellular part), GO:0043656 (host intracellular region), GO:0033655 (host cell cytoplasm part), GO:0033647 (host intracellular organelle), GO:0020002 (host cell plasma membrane), GO:0030430 (host cell cytoplasm), GO:0033648 (host intracellular membrane-bounded organelle), GO:0044164 (host cell cytosol)