Protein: FACT complex subunit SPT16 (Q5A1D5)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II (By similarity)
Subcellular location: Nucleus. Chromosome
Sequence: MSEVNIDAGLFYKRLSIFQKQLTANNIPQALIIVGARSDDNTYKKSTVLQNWLLGYEFIHTAIYITDKKCIFITSEGKSKHLKHLTNQKPDLVELWIRTKDVEHNKQLFIKLLETMTKLDSKYGKILKDKYDGKFIDEWNQILNDDNNNNNNNTTNDHALSAVDLAVTVSQALAVKDSEEFNNTKIASNASVVMMDTFVNDMMIIVDDEKKITNSQLTDQIEDKIENNKWYLKTKLGKNLLQSIKDFDPEYLEYCYSPIIQSGGDYDLKPSAVSTDKPLIGEGVILSSIGLRYKSYCSNIARTFLIDPTSEMETNYDFLLQLQKYIVDNLLKDGVPANKVYQDTIDYIKKERPDLVNHFTKNCGWLLGMEFRDSTFILNAKTTDRKLTTGQIISLTIGFNNLSNDKNDKNDKNDNKTNHQKNKQTYALLLTDTIKITDDSSILLTNYSKDRAAISFSFNDDNETQKENNNNNNKRPGLSQTSNTTALKLESTENTAILKSKLRHENTNADDANSEKLRQEIQIKLHEKRLQEGLARFSKADATDADDFKPIFKKYESYVRESQIPNSVNDLKIHIDYKNQTIILPISGRPVPFHINSYKSGSQNEEGDFTYLRLNFNSPGAGGNVTKKQELPYEDSPDNSFLRSITIRSRDRQRMVDVYKAIQDLKKDSVKREQEKKQMADVITQANLIELKGSRVKKLNNVFIRPTPDTKKIGGVLQIHENGLRYQSQPQSQSNFKNDQRVDVLFSNIKHLFFQPCKDELIVLIHCHLKNPIMIGKRKTFDVQFYREASDMAFDETGGRKRKYRYGDEDELQQEQEERRRKALLDKEFKGFAELIADSSHGMVDLDIPFRELGFQGVPFRSSVLCVPTRDCLVQLIDPPYLVVTLEEIEIAHLERVQFGLKNFDLVFVFKDFNKPVVHINTIPVELLEDVKSWLTDVDIPISEGQMNLNWVQIMKTVLADPYQFFIDGGWAFLTGQGESDEEEESDEESDFRVSDEDPQDEDEESDDYASEEESDDYSGSDDDGSGGGGDDDDDDSESGEDWDALERKAAKADRNSGFD
InterPro domains: - IPR040258: FACT complex subunit Spt16 (family) [1-1058]
- IPR029148: FACT complex subunit SPT16, N-terminal lobe domain (domain) [6-169]
- IPR013953: FACT complex subunit SPT16, middle domain (domain) [573-726]
- IPR011993: PH-like domain superfamily (homologous_superfamily) [848-979]
- IPR013719: Histone chaperone RTT106/FACT complex subunit SPT16-like, middle domain (domain) [855-945]
- IPR056595: FACT complex subunit SPT16, PH-like domain (domain) [698-835]
- IPR029149: Creatinase/Aminopeptidase P/Spt16, N-terminal (homologous_superfamily) [1-171]
- IPR033825: FACT complex subunit Spt16, peptidase M24-like domain (domain) [181-446]
- IPR036005: Creatinase/aminopeptidase-like (homologous_superfamily) [174-458]
- IPR000994: Peptidase M24 (domain) [210-402]
Interaction partners: - FACT complex subunit POB3
- Cdc73p
- Transcription elongation factor SPT5
- RNA polymerase-associated protein CTR9
- Transcription elongation factor SPT6
- RNA polymerase-associated protein LEO1
- RNA polymerase II-associated protein 1
- Transcription elongation factor SPT4
- Rtf1p
- DNA-directed RNA polymerase subunit
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0044877 (protein-containing complex binding), GO:0003682 (chromatin binding), GO:0005515 (protein binding), GO:0031491 (nucleosome binding), GO:0042393 (histone binding)
Biological Process (BP): GO:0008150 (biological process), GO:0040007 (growth), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0009605 (response to external stimulus), GO:0051716 (cellular response to stimulus), GO:0030447 (filamentous growth), GO:0006950 (response to stress), GO:0009607 (response to biotic stimulus), GO:0007154 (cell communication), GO:0009991 (response to extracellular stimulus), GO:0031668 (cellular response to extracellular stimulus), GO:0044182 (filamentous growth of a population of unicellular organisms), GO:0036180 (filamentous growth of a population of unicellular organisms in response to biotic stimulus), GO:0042594 (response to starvation), GO:0071496 (cellular response to external stimulus), GO:0033554 (cellular response to stress), GO:0036170 (filamentous growth of a population of unicellular organisms in response to starvation), GO:0009267 (cellular response to starvation), GO:0031669 (cellular response to nutrient levels), GO:0031667 (response to nutrient levels)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000785 (chromatin), GO:0140513 (nuclear protein-containing complex), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0000791 (euchromatin), GO:0043229 (intracellular organelle), GO:0008023 (transcription elongation factor complex), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005694 (chromosome), GO:0005634 (nucleus)