Protein: Dipeptidyl-peptidase 7 (DPP-7) (DPP7) (EC 3.4.14.-) (B2RKV3)
Organism: Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561)
Function: Catalyzes the removal of dipeptides from the N-terminus of oligopeptides. Shows a broad specificity for both aliphatic and aromatic residues in the P1 position, with glycine or proline being not acceptable in this position. Most potently cleaves the synthetic substrate Met-Leu-methylcoumaryl-7-amide (Met-Leu-MCA), Leu-Arg-MCA and Lys-Ala-MCA to a lesser extent. Is likely involved in amino acid metabolism and bacterial growth of asaccharolytic P.gingivalis, that utilizes amino acids from extracellular proteinaceous nutrients as energy and carbon sources.
Subcellular location: Cell outer membrane {ECO:0000269|PubMed:11096098}.
Sequence: MQMKLKSILLGAALLLGASGVAKADKGMWLLNELNQENLDRMRELGFTLPLDSLYSFDKPSIANAVVIFGGGCTGITVSDQGLIFTNHHCGYGAIQSQSTVDHDYLRDGFVSRTMGEELPIPGLSVKYLRKIVKVTDKVEGQLKGITDEMERLRKAQEVCQELAKKENADENQLCIVEPFYSNNEYFLIVYDVFKDVRMVFAPPSSVGKFGGDTDNWMWPRHTGDFSVFRVYAGADNRPAEYSKDNKPYKPVYFAAVSMQGYKADDYAMTIGFPGSTDRYLTSWGVEDRIENENNPRIEVRGIKQGIWKEAMSADQATRIKYASKYAQSANYWKNSIGMNRGLARLDVIGRKRAEERAFADWIRKNGKSAVYGDVLSSLEKAYKEGAKANREMTYLSETLFGGTEVVRFAQFANALATNPDAHAGILKSLDDKYKDYLPSLDRKVLPAMLDIVRRRIPADKLPDIFKNVIDKKFKGDTKKYADFVFDKSVVPYSDKFHAMLKSMDKEKFAKAIEKDPAVELSKSVIAAARAIQADAMANAYAIEKGKRLFFAGLREMYPGRALPSDANFTMRMSYGSIKGYEPQDGAWYNYHTTGKGVLEKQDPKSDEFAVQENILDLFRTKNYGRYAENGQLHIAFLSNNDITGGNSGSPVFDKNGRLIGLAFDGNWEAMSGDIEFEPDLQRTISVDIRYVLFMIDKWGQCPRLIQELKLI
InterPro domains: - IPR019500: Peptidase S46 (family) [15-711]
- IPR043504: Peptidase S1, PA clan, chymotrypsin-like fold (homologous_superfamily) [593-689]
- IPR009003: Peptidase S1, PA clan (homologous_superfamily) [53-703]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0033218 (amide binding), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0042277 (peptide binding), GO:0008233 (peptidase activity), GO:0008238 (exopeptidase activity), GO:0008239 (dipeptidyl-peptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0032502 (developmental process), GO:0040007 (growth), GO:0009987 (cellular process), GO:0048589 (developmental growth), GO:0048856 (anatomical structure development), GO:0048869 (cellular developmental process), GO:0044237 (cellular metabolic process), GO:0016049 (cell growth), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0048588 (developmental cell growth), GO:0043603 (amide metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0044248 (cellular catabolic process), GO:0006518 (peptide metabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0043171 (peptide catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0009986 (cell surface)