Protein: Penicillin-binding protein 1B (PBP-1b) (PBP1b) (Murein polymerase) [Includes: Penicillin-insensitive transglycosylase (EC 2.4.99.28) (Peptidoglycan TGase) (Peptidoglycan glycosyltransferase); Penicillin-sensitive transpeptidase (EC 3.4.16.4) (DD-transpeptidase)] (P02919)
Organism: Escherichia coli (strain K12)
Function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).
Subcellular location: Cell inner membrane; Single-pass type II membrane protein.
Sequence: MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEEPMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLPAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDMLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVDALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQAFQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRILPVWTSDPQSLCQQSEMQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN
InterPro domains: - IPR028166: Bifunctional transglycosylase second domain (domain) [113-197]
- IPR036950: Penicillin binding protein transglycosylase domain (homologous_superfamily) [201-404]
- IPR011813: Penicillin-binding protein 1B (family) [13-843]
- IPR023346: Lysozyme-like domain superfamily (homologous_superfamily) [196-403]
- IPR032730: Transglycosylase PBP1b, N-terminal transmembrane domain (domain) [1-79]
- IPR050396: Glycosyltransferase 51/Transpeptidase (family) [46-830]
- IPR012338: Beta-lactamase/transpeptidase-like (homologous_superfamily) [103-777]
- IPR001460: Penicillin-binding protein, transpeptidase (domain) [473-714]
- IPR001264: Glycosyl transferase, family 51 (domain) [209-379]
Interaction partners: - Peptidoglycan D,D-transpeptidase FtsI
- Cell division protein FtsN
- Penicillin-binding protein activator LpoB
- Probable peptidoglycan glycosyltransferase FtsW
- Peptidoglycan D,D-transpeptidase MrdA
- Penicillin-binding protein 1A
- Peptidoglycan glycosyltransferase MrdB
- Cell division coordinator CpoB
- D-alanyl-D-alanine carboxypeptidase DacB
- Penicillin-binding protein activator LpoA
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0016740 (transferase activity), GO:0016787 (hydrolase activity), GO:1901681 (sulfur compound binding), GO:0097159 (organic cyclic compound binding), GO:0033218 (amide binding), GO:0140096 (catalytic activity, acting on a protein), GO:0043167 (ion binding), GO:0005515 (protein binding), GO:0016757 (glycosyltransferase activity), GO:0008233 (peptidase activity), GO:0043177 (organic acid binding), GO:0017171 (serine hydrolase activity), GO:0043168 (anion binding), GO:0016758 (hexosyltransferase activity), GO:0008236 (serine-type peptidase activity), GO:0031406 (carboxylic acid binding), GO:0008238 (exopeptidase activity), GO:0070008 (serine-type exopeptidase activity), GO:0004180 (carboxypeptidase activity), GO:0033293 (monocarboxylic acid binding), GO:0004185 (serine-type carboxypeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0033554 (cellular response to stress), GO:0019538 (protein metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0031323 (regulation of cellular metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006412 (translation), GO:0009059 (macromolecule biosynthetic process), GO:0034248 (regulation of amide metabolic process), GO:0006518 (peptide metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0006446 (regulation of translational initiation), GO:0031326 (regulation of cellular biosynthetic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0043555 (regulation of translation in response to stress), GO:0010467 (gene expression), GO:0010468 (regulation of gene expression), GO:0043043 (peptide biosynthetic process), GO:0006414 (translational elongation), GO:2000112 (regulation of cellular macromolecule biosynthetic process), GO:0006417 (regulation of translation), GO:0010608 (post-transcriptional regulation of gene expression)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)