Protein: Histone acetyltransferase ESA1 (EC 2.3.1.48) (Protein 2-hydroxyisobutyryltransferase ESA1) (EC 2.3.1.-) (Protein acetyltransferase ESA1) (EC 2.3.1.-) (Protein crotonyltransferase ESA1) (EC 2.3.1.-) (Q08649)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Catalytic component of the NuA4 histone acetyltransferase (HAT), a multiprotein complex involved in epigenetic transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4, H3, H2B, H2A and H2A variant H2A.Z. Acetylates histone H4 to form H4K5ac, H4K8ac, H4K12ac and H4K16ac, histone H3 to form H3K14ac, histone H2B to form H2BK16ac, histone H2A to form H2AK4ac and H2AK7ac, and histone variant H2A.Z to form H2A.ZK14ac. Acetylation of histones gives a specific tag for epigenetic transcription initiation and elongation. Acetylation of histone H4 is essential for DNA double-strand break repair through homologous recombination. Involved in cell cycle progression. Recruitment to promoters depends on H3K4me. Also acetylates non-histone proteins, such as ATG3 and PAH1. Regulates autophagy by acetylating ATG3, controlling interaction the interaction between ATG3 and ATG8 and ATG8 lipidation. Acts as a regulator of fatty-acid-induced triacylglycerol synthesis by catalyzing acetylation of PAH1, thereby promoting the synthesis of diacylglycerol. In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as 2-hydroxyisobutanoyl-CoA (2-hydroxyisobutyryl-CoA) or (2E)-butenoyl-CoA (crotonyl-CoA), and is able to mediate protein 2-hydroxyisobutyrylation and crotonylation, respectively. Catalyzes histone crotonylation.
Sequence: MSHDGKEEPGIAKKINSVDDIIIKCQCWVQKNDEERLAEILSINTRKAPPKFYVHYVNYNKRLDEWITTDRINLDKEVLYPKLKATDEDNKKQKKKKATNTSETPQDSLQDGVDGFSRENTDVMDLDNLNVQGIKDENISHEDEIKKLRTSGSMTQNPHEVARVRNLNRIIMGKYEIEPWYFSPYPIELTDEDFIYIDDFTLQYFGSKKQYERYRKKCTLRHPPGNEIYRDDYVSFFEIDGRKQRTWCRNLCLLSKLFLDHKTLYYDVDPFLFYCMTRRDELGHHLVGYFSKEKESADGYNVACILTLPQYQRMGYGKLLIEFSYELSKKENKVGSPEKPLSDLGLLSYRAYWSDTLITLLVEHQKEITIDEISSMTSMTTTDILHTAKTLNILRYYKGQHIIFLNEDILDRYNRLKAKKRRTIDPNRLIWKPPVFTASQLRFAW
InterPro domains: - IPR000953: Chromo/chromo shadow domain (domain) [33-86]
- IPR040706: MYST, zinc finger domain (domain) [164-218]
- IPR016181: Acyl-CoA N-acyltransferase (homologous_superfamily) [163-433]
- IPR002717: Histone acetyltransferase domain, MYST-type (domain) [162-433]
- IPR025995: RNA binding activity-knot of a chromodomain (domain) [21-74]
- IPR016197: Chromo-like domain superfamily (homologous_superfamily) [10-111]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [341-421]
- IPR050603: MYST family histone acetyltransferases (family) [23-439]
Interaction partners: - Enhancer of polycomb-like protein 1
- Chromatin modification-related protein EAF1
- Actin-related protein 4
- SAGA complex/NuA4 acetyltransferase complex subunit TRA1
- Chromatin modification-related protein YNG2
- Chromatin modification-related protein EAF6
- SWR1-complex protein 4
- Protein AF-9 homolog
- Chromatin modification-related protein EAF3
- Histone H4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0061733 (peptide-lysine-N-acetyltransferase activity), GO:0016746 (acyltransferase activity), GO:0004402 (histone acetyltransferase activity), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups), GO:0016407 (acetyltransferase activity), GO:0016410 (N-acyltransferase activity), GO:0008080 (N-acetyltransferase activity), GO:0034212 (peptide N-acetyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0048584 (positive regulation of response to stimulus), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0009893 (positive regulation of metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0048523 (negative regulation of cellular process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0044085 (cellular component biogenesis), GO:0051129 (negative regulation of cellular component organization), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0080134 (regulation of response to stress), GO:0080135 (regulation of cellular response to stress), GO:0060255 (regulation of macromolecule metabolic process), GO:0051130 (positive regulation of cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044089 (positive regulation of cellular component biogenesis), GO:0043170 (macromolecule metabolic process), GO:0044087 (regulation of cellular component biogenesis), GO:2001022 (positive regulation of response to DNA damage stimulus), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0033554 (cellular response to stress), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0051128 (regulation of cellular component organization), GO:0044260 (cellular macromolecule metabolic process), GO:0051052 (regulation of DNA metabolic process), GO:0051054 (positive regulation of DNA metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:1902275 (regulation of chromatin organization), GO:0010628 (positive regulation of gene expression), GO:0045739 (positive regulation of DNA repair), GO:2001020 (regulation of response to DNA damage stimulus), GO:0010468 (regulation of gene expression), GO:0022607 (cellular component assembly), GO:1905268 (negative regulation of chromatin organization), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0031453 (positive regulation of heterochromatin formation), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0033043 (regulation of organelle organization), GO:0006259 (DNA metabolic process), GO:0043933 (protein-containing complex organization), GO:0006996 (organelle organization), GO:0010638 (positive regulation of organelle organization), GO:2000781 (positive regulation of double-strand break repair), GO:0000018 (regulation of DNA recombination), GO:0120262 (negative regulation of heterochromatin organization), GO:0045911 (positive regulation of DNA recombination), GO:0031445 (regulation of heterochromatin formation), GO:0006282 (regulation of DNA repair), GO:0065003 (protein-containing complex assembly), GO:0071824 (protein-DNA complex subunit organization), GO:0006281 (DNA repair), GO:0051276 (chromosome organization), GO:2001252 (positive regulation of chromosome organization), GO:0031452 (negative regulation of heterochromatin formation), GO:0033044 (regulation of chromosome organization), GO:0120261 (regulation of heterochromatin organization), GO:1905269 (positive regulation of chromatin organization), GO:0120263 (positive regulation of heterochromatin organization), GO:0034508 (centromere complex assembly), GO:2000779 (regulation of double-strand break repair), GO:0065004 (protein-DNA complex assembly), GO:0010569 (regulation of double-strand break repair via homologous recombination), GO:1905168 (positive regulation of double-strand break repair via homologous recombination), GO:0006302 (double-strand break repair)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000785 (chromatin), GO:0140535 (intracellular protein-containing complex), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0031248 (protein acetyltransferase complex), GO:1990234 (transferase complex), GO:0043229 (intracellular organelle), GO:0000123 (histone acetyltransferase complex), GO:0043228 (non-membrane-bounded organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:1902562 (H4 histone acetyltransferase complex), GO:0043232 (intracellular non-membrane-bounded organelle), GO:1902493 (acetyltransferase complex), GO:0043189 (H4/H2A histone acetyltransferase complex), GO:0005634 (nucleus), GO:0005694 (chromosome), GO:0035267 (NuA4 histone acetyltransferase complex)