Protein: DNA replication factor Cdt1 (Double parked homolog) (DUP) (Q9H211)
Organism: Homo sapiens (Human)
Function: Required for both DNA replication and mitosis. DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC). Required also for mitosis by promoting stable kinetochore-microtubule attachments. Potential oncogene (By similarity).
Subcellular location: Nucleus {ECO:0000269|PubMed:11125146, ECO:0000269|PubMed:26842564}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:22581055}. Note=Transiently localizes to kinetochores during prometaphase and metaphase. {ECO:0000269|PubMed:22581055}.
Sequence: MEQRRVTDFFARRRPGPPRIAPPKLACRTPSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSVDEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTISELASCLQRARELGARVRALKASAQDAGESCTPEAEGRPEEPCGEKAPAYQRFHALAQPGLPGLVLPYKYQVLAEMFRSMDTIVGMLHNRSETPTFAKVQRGVQDMMRRRFEECNVGQIKTVYPASYRFRQERSVPTFKDGTRRSDYQLTIEPLLEQEADGAAPQLTASRLLQRRQIFSQKLVEHVKEHHKAFLASLSPAMVVPEDQLTRWHPRFNVDEVPDIEPAALPQPPATEKLTTAQEVLARARNLISPRMEKALSQLALRSAAPSSPGSPRPALPATPPATPPAASPSALKGVSQDLLERIRAKEAQKQLAQMTRCPEQEQRLQRLERLPELARVLRSVFVSERKPALSMEVACARMVGSCCTIMSPGEMEKHLLLLSELLPDWLSLHRIRTDTYVKLDKAADLAHITARLAHQTRAEEGL
InterPro domains: - IPR036390: Winged helix DNA-binding domain superfamily (homologous_superfamily) [167-352]
- IPR038090: DNA replication factor Cdt1, C-terminal WH domain superfamily (homologous_superfamily) [408-545]
- IPR045173: DNA replication factor Cdt1 (family) [49-539]
- IPR032054: DNA replication factor Cdt1, C-terminal (domain) [415-537]
- IPR014939: CDT1 Geminin-binding domain-like (domain) [168-352]
Interaction partners: - Origin recognition complex subunit 1
- Geminin
- DNA replication licensing factor MCM5
- DNA replication licensing factor MCM4
- Origin recognition complex subunit 4
- DNA replication licensing factor MCM7
- DNA replication licensing factor MCM6
- DNA replication licensing factor MCM2
- Origin recognition complex subunit 2
- DNA replication licensing factor MCM3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003677 (DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0019222 (regulation of metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0006259 (DNA metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0051052 (regulation of DNA metabolic process), GO:0006260 (DNA replication), GO:0006275 (regulation of DNA replication), GO:0090329 (regulation of DNA-templated DNA replication), GO:0006261 (DNA-templated DNA replication)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)