Protein: MICAL-like protein 1 (E1BBG2)
Organism: Bos taurus (Bovine)
Function: Probable lipid-binding protein with higher affinity for phosphatidic acid, a lipid enriched in recycling endosome membranes. On endosome membranes, may act as a downstream effector of Rab proteins recruiting cytosolic proteins to regulate membrane tubulation. May be involved in a late step of receptor-mediated endocytosis regulating for instance endocytosed-EGF receptor trafficking. Alternatively, may regulate slow endocytic recycling of endocytosed proteins back to the plasma membrane. May indirectly play a role in neurite outgrowth (By similarity).
Subcellular location: Recycling endosome membrane {ECO:0000250|UniProtKB:Q8N3F8}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q8N3F8}. Late endosome membrane {ECO:0000250|UniProtKB:Q8N3F8}. Cell projection, cilium membrane {ECO:0000250|UniProtKB:Q8N3F8}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q8N3F8}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:Q8N3F8}. Note=Localization to late endosomes is actin-dependent. Association to tubular recycling endosomes is regulated by RAB35 and ARF6. Interaction with tubulins achors MICALL1 to the centriole. {ECO:0000250|UniProtKB:Q8N3F8}.
Sequence: MAGPRGALLAWCRRQCEGYRGVDIRDLSSSFRDGLAFCAILHRHRPDLLDFDSLSKDNVFENNRLAFEVAEKELGIPALLDPNDMVSMSVPDCLSIMTYVSQYYNHFAGSGPAGVSSPRKGLVLSSPPSEASTPADPGDRAQGEECSSGSLSKQGSHRTPSSTCAACQQHVHLVQRYLADGKLYHRHCFRCRRCSSTLLPGAYRNGPEEGTFVCAEHCARLGPSGRSGARPGTPPQPKQQQLTEEAKEVEGGSPSPKATAGAEADVPKASPEGRPQVPTKPRVPGRPQELASPPASRPTPAPRKASESTAPTPPTPRPRSSLQQENLVEQGGGSGLVNGKLQEPPIPKPRGTPKLSERTPAPRKDPPWITLVQAEPKKKPAPLPPSSSPGPPPGQEGRQVENGGVDKAAPRGPEPKPYNPFEEEEEEPPAAPSPAPGPAPTPPESTPKSLHPWYGITPTSSPKTKKRPAPRAPSTSPLTLHASRLSRSEPPSATPSPALSVESLSSESSSQAPSEELLEPPVVPKSSSEPAVHAPGTPGTSASLSANSSLSSSGELVQPSMDRTPQASPGLAPNSRGSPGPPPAKPCSGTAPTPLVLVGDKSPAPSPGTSSPQLQVKSSCKENPFNRKPSPTASPSVKKATKGSKPARPPAPGHGFPLIKRKVQSDQYIPEEDIHGEIDTIERQLDALEHRGVLLEEKLRGGVNEGREDDMLVDWFKLIHEKHLLVRRESELIYVFKQQNLEQRQADVEYELRCLLNKPEKDWTEEDRGREKVLMQELVTLIEQRNAIVNCLDEDRQREEEEDKMLEAMIKKKEFQKETEPEGKKKGKFKTMKVLKLLGNKRDTKSKCPGDRS
InterPro domains: - IPR036872: CH domain superfamily (homologous_superfamily) [2-109]
- IPR050540: F-actin Monooxygenase Mical (family) [7-813]
- IPR022735: bMERB domain (domain) [661-808]
- IPR001715: Calponin homology domain (domain) [2-108]
- IPR001781: Zinc finger, LIM-type (domain) [162-224]
Interaction partners: - Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein
- ArfGAP with coiled-coil, ankyrin repeat and PH domains 2.
- Ras-related protein Rab-35
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0033036 (macromolecule localization), GO:0046907 (intracellular transport), GO:0006810 (transport), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0051668 (localization within membrane), GO:0045184 (establishment of protein localization), GO:0006886 (intracellular protein transport), GO:0071705 (nitrogen compound transport), GO:0016197 (endosomal transport), GO:0098876 (vesicle-mediated transport to the plasma membrane), GO:0015031 (protein transport), GO:0071702 (organic substance transport), GO:0016192 (vesicle-mediated transport), GO:0008104 (protein localization), GO:0090150 (establishment of protein localization to membrane), GO:0061024 (membrane organization), GO:0072657 (protein localization to membrane), GO:0033365 (protein localization to organelle), GO:0006612 (protein targeting to membrane), GO:0006897 (endocytosis), GO:0006605 (protein targeting), GO:0032456 (endocytic recycling), GO:0007009 (plasma membrane organization), GO:0006898 (receptor-mediated endocytosis), GO:0036010 (protein localization to endosome), GO:0097320 (plasma membrane tubulation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)