Protein: Nuclear pore complex protein Nup50 (50 kDa nucleoporin) (Nuclear pore-associated protein 60 kDa-like) (Nucleoporin Nup50) (Q9JIH2)
Organism: Mus musculus (Mouse)
Function: Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with regulatory proteins of cell cycle progression including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.
Subcellular location: Nucleus, nuclear pore complex. Nucleus membrane {ECO:0000250|UniProtKB:O08587}; Peripheral membrane protein {ECO:0000250|UniProtKB:O08587}; Nucleoplasmic side {ECO:0000250|UniProtKB:O08587}. Note=Localizes to the nucleoplasmic fibrils of the nuclear pore complex. Dissociates from the NPC structure early during prophase of mitosis. Associates with the newly formed nuclear membrane during telophase. In the testis, the localization changes during germ cell differentiation from the nuclear surface in spermatocytes to the whole nucleus (interior) in spermatids and back to the nuclear surface in spermatozoa. {ECO:0000250|UniProtKB:O08587, ECO:0000250|UniProtKB:Q9UKX7}.
Sequence: MAKRVAEKELTDRNWDEEDEVEEMGTFSVASEEVMKNRAVKKAKRRNVGFESDSGGAFKGFKGLVVPSGGGGFSGFGGSGGKPLEGLTNGNSTDNATPFSNVKTAAEPKAAFGSFAVNGPTTLVDKKISSPKCNNSNQPPSSGPASSTACPGNAYHKQLAGLNCSVRDWIVKHVNTNPLCDLTPIFKDYERYLATIEKQLENGGGSSSESQTDRATAGMEPPSLFGSTKLQQESPFSFHGNKAEDTSEKVEFTAEKKSDAAQGATSASFSFGKKIESSALGSLSSGSLTGFSFSAGSSSLFGKDAAQSKAASSLFSAKASESPAGGGSSECRDGEEEENDEPPKVVVTEVKEEDAFYSKKCKLFYKKDNEFKEKGVGTLHLKPTATQKTQLLVRADTNLGNILLNVLIAPNMPCTRTGKNNVLIVCVPNPPLDEKQPTLPATMLIRVKTSEDADELHKILLEKKDA
InterPro domains: - IPR015007: Nuclear pore complex, NUP2/50/61 (domain) [2-64]
- IPR045255: Ran binding protein RanBP1-like (family) [20-462]
- IPR000156: Ran binding domain (domain) [340-466]
- IPR011993: PH-like domain superfamily (homologous_superfamily) [345-466]
Interaction partners: - Nuclear pore complex protein Nup153
- Importin subunit alpha-1
- Nuclear pore complex protein Nup98-Nup96
- Nuclear pore complex protein Nup88
- Nuclear pore complex protein Nup214
- Nuclear pore complex protein Nup155
- Nucleoporin Nup43
- Nuclear pore complex protein Nup93
- Nuclear pore complex protein Nup107
- Nucleoporin 205
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0023057 (negative regulation of signaling), GO:0048524 (positive regulation of viral process), GO:0019222 (regulation of metabolic process), GO:0009607 (response to biotic stimulus), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0023051 (regulation of signaling), GO:0050792 (regulation of viral process), GO:0006950 (response to stress), GO:0048523 (negative regulation of cellular process), GO:0051707 (response to other organism), GO:0048585 (negative regulation of response to stimulus), GO:0009892 (negative regulation of metabolic process), GO:0009968 (negative regulation of signal transduction), GO:0010648 (negative regulation of cell communication), GO:0045069 (regulation of viral genome replication), GO:0045070 (positive regulation of viral genome replication), GO:0043207 (response to external biotic stimulus), GO:1903900 (regulation of viral life cycle), GO:0010646 (regulation of cell communication), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009617 (response to bacterium), GO:0009966 (regulation of signal transduction), GO:0006952 (defense response), GO:0060255 (regulation of macromolecule metabolic process), GO:0009615 (response to virus), GO:0098542 (defense response to other organism), GO:1904893 (negative regulation of receptor signaling pathway via STAT), GO:0140546 (defense response to symbiont), GO:0051607 (defense response to virus), GO:0042742 (defense response to bacterium), GO:0032480 (negative regulation of type I interferon production), GO:1904892 (regulation of receptor signaling pathway via STAT), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression), GO:0032479 (regulation of type I interferon production), GO:0046425 (regulation of receptor signaling pathway via JAK-STAT), GO:0046426 (negative regulation of receptor signaling pathway via JAK-STAT)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140513 (nuclear protein-containing complex), GO:0031975 (envelope), GO:0012505 (endomembrane system), GO:0005643 (nuclear pore), GO:0043229 (intracellular organelle), GO:0031967 (organelle envelope), GO:0005635 (nuclear envelope), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)