Protein: Palmitoyltransferase ERF2 (EC 2.3.1.225) (DHHC cysteine-rich domain-containing protein ERF2) (Ras protein acyltransferase) (Q06551)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: The ERF2-SHR5 complex is a palmitoyltransferase specific for Ras proteins. Palmitoylates RAS2, which is required for its proper plasma membrane localization.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:10490616, ECO:0000269|PubMed:12379641}; Multi-pass membrane protein {ECO:0000269|PubMed:10490616, ECO:0000269|PubMed:12379641}.
Sequence: MALVSRRSTRSESTSITKEEHTGEGSLTKLFFRWLVTLEGDQDINDGKGYISLPNVSNYIFFLGGRFRTVKGAKPLWLGVLLAIVCPMVLFSIFEAHKLWHTQNGYKVLVIFFYYFWVITLASFIRTATSDPGVLPRNIHLSQLRNNYQIPQEYYNLITLPTHSSISKDITIKYCPSCRIWRPPRSSHCSTCNVCVMVHDHHCIWVNNCIGKRNYRFFLIFLLGAILSSVILLTNCAIHIARESGGPRDCPVAILLLCYAGLTLWYPAILFTYHIFMAGNQQTTREFLKGIGSKKNPVFHRVVKEENIYNKGSFLKNMGHLMLEPRGPSFVSARKPHEAGDWRFMDLSPAHSFEKIQKI
InterPro domains: - IPR001594: Palmitoyltransferase, DHHC domain (domain) [171-289]
- IPR039859: Palmitoyltransferase PFA4/ZDHHC16/ZDHHC20/ERF2-like (family) [84-328]
Interaction partners: - Ras modification protein ERF4
- GTP-binding protein RHO2
- Serine palmitoyltransferase 1
- Serine palmitoyltransferase 2
- Protein ORM1
- Serine palmitoyltransferase-regulating protein TSC3
- Phosphatidylinositol-3-phosphatase SAC1
- Protein ORM2
- Ras-like protein 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016746 (acyltransferase activity), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups), GO:0016409 (palmitoyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0036211 (protein modification process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0043412 (macromolecule modification), GO:0034645 (cellular macromolecule biosynthetic process), GO:0042157 (lipoprotein metabolic process), GO:0006497 (protein lipidation), GO:0043543 (protein acylation), GO:0018193 (peptidyl-amino acid modification), GO:0042158 (lipoprotein biosynthetic process), GO:0018198 (peptidyl-cysteine modification), GO:0018345 (protein palmitoylation), GO:0018231 (peptidyl-S-diacylglycerol-L-cysteine biosynthetic process from peptidyl-cysteine), GO:0018230 (peptidyl-L-cysteine S-palmitoylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)