Protein: Carbohydrate sulfotransferase 5 (EC 2.8.2.-) (Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4) (GST4) (Intestinal N-acetylglucosamine-6-O-sulfotransferase) (I-GlcNAc6ST) (Intestinal GlcNAc-6-sulfotransferase) (mIGn6ST) (N-acetylglucosamine 6-O-sulfotransferase 3) (GlcNAc6ST-3) (Gn6st-3) (Q9QUP4)
Organism: Mus musculus (Mouse)
Function: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures. May also have activity toward O-linked sugars of mucin-type acceptors.
Subcellular location: Golgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}. Note=Golgi membrane, early secretory pathway. {ECO:0000250}.
Sequence: MRLPRFSSTVMLSLLMVQTGILVFLVSRQVPSSPAGLGERVHVLVLSSWRSGSSFVGQLFSQHPDVFYLMEPAWHVWDTLSQGSAPALHMAVRDLIRSVFLCDMDVFDAYLPWRRNISDLFQWAVSRALCSPPVCEAFARGNISSEEVCKPLCATRPFGLAQEACSSYSHVVLKEVRFFNLQVLYPLLSDPALNLRIVHLVRDPRAVLRSREQTAKALARDNGIVLGTNGTWVEADPRLRVVNEVCRSHVRIAEAALHKPPPFLQDRYRLVRYEDLARDPLTVIRELYAFTGLGLTPQLQTWIHNITHGSGPGARREAFKTTSRDALSVSQAWRHTLPFAKIRRVQELCGGALQLLGYRSVHSELEQRDLSLDLLLPRGMDSFKWASSTEKQPES
InterPro domains: - IPR051135: Gal/GlcNAc/GalNAc Sulfotransferase (family) [38-378]
- IPR016469: Carbohydrate sulfotransferase (family) [19-384]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [33-367]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016782 (transferase activity, transferring sulphur-containing groups), GO:0008146 (sulfotransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0002376 (immune system process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0048870 (cell motility), GO:0050900 (leukocyte migration), GO:0007155 (cell adhesion), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0002682 (regulation of immune system process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0040012 (regulation of locomotion), GO:0048522 (positive regulation of cellular process), GO:1901564 (organonitrogen compound metabolic process), GO:2000145 (regulation of cell motility), GO:0002685 (regulation of leukocyte migration), GO:0098609 (cell-cell adhesion), GO:0019538 (protein metabolic process), GO:0030155 (regulation of cell adhesion), GO:0045785 (positive regulation of cell adhesion), GO:0045123 (cellular extravasation), GO:0043170 (macromolecule metabolic process), GO:0006790 (sulfur compound metabolic process), GO:0016477 (cell migration), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0022409 (positive regulation of cell-cell adhesion), GO:0022407 (regulation of cell-cell adhesion), GO:0002691 (regulation of cellular extravasation), GO:0050901 (leukocyte tethering or rolling), GO:0051923 (sulfation), GO:0030334 (regulation of cell migration), GO:0007159 (leukocyte cell-cell adhesion), GO:0061756 (leukocyte adhesion to vascular endothelial cell), GO:1903037 (regulation of leukocyte cell-cell adhesion), GO:1903039 (positive regulation of leukocyte cell-cell adhesion), GO:1904996 (positive regulation of leukocyte adhesion to vascular endothelial cell), GO:1904994 (regulation of leukocyte adhesion to vascular endothelial cell)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)