Protein: Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial (EC 1.14.13.-) (2-methoxy-6-polyprenolphenol 4-hydroxylase) (EC 1.14.15.-) (Q9Y7Z9)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3-polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin/ferredoxin reductase system to COQ6.
Subcellular location: Mitochondrion inner membrane {ECO:0000255|HAMAP-Rule:MF_03193, ECO:0000269|PubMed:16823372, ECO:0000269|PubMed:24911838}; Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_03193}; Matrix side {ECO:0000255|HAMAP-Rule:MF_03193}.
Sequence: MSSLVLRGVSRVEMPTISPTLGIYTRKFASQKILQRQFDVVIVGSGPVGLALAAGLQSNPVTQSLKVGLLDIQDTMKLKDWKFETYSNRCSSLTNHTRMFFDKIGAWDFARKDRIQPFQHILASDGLTNSSIHLDQKPGSEPMAFMSENVNLQYALLNSIIDKMNNNIKKPNLEFLMPCTITKLSKGENIYRTHIHTTTHGELTTKLLIGADGRNSIVRKYANISMPGWNYLTHAVVGTLKIDPLKGPAVAFQRFLPTGPLAYLPLPDNNATFVWSTRPHIASKLLRLPEETFVKFLNASFRLDYPDLSYLYQMDFSEPSKVNEQLDWRLQVKRNSNMQVPPVITEIVSGSRAAFPLRLAHVDEYVKEGIALCGDAAHNTHPLAGQGLNTGIQDVESLISALSFAIKHGQDIGSVFSLQPYFRDRYFKNHVYLGVVDKFHKLYAMENPVVTSVRTLGLSLFDRSASLKNFILSTVANGI
InterPro domains: - IPR018168: Ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6, conserved site (conserved_site) [381-394]
- IPR010971: Ubiquinone biosynthesis hydroxylase UbiH/COQ6 (family) [39-475]
- IPR051205: Ubiquinone biosynthesis monooxygenase UbiH/COQ6 (family) [35-478]
- IPR002938: FAD-binding domain (domain) [38-407]
- IPR000689: Ubiquinone biosynthesis monooxygenase COQ6 (family) [25-477]
- IPR036188: FAD/NAD(P)-binding domain superfamily (homologous_superfamily) [36-478]
Interaction partners: - Ubiquinone biosynthesis O-methyltransferase, mitochondrial
- 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial
- 5-demethoxyubiquinone hydroxylase, mitochondrial
- Ubiquinone biosynthesis protein coq4, mitochondrial
- 4-hydroxybenzoate polyprenyltransferase, mitochondrial
- Atypical kinase COQ8, mitochondrial
- Ubiquinone biosynthesis protein coq9
- Ubiquinone biosynthesis protein coq11
- Thiol-specific monooxygenase
- Decaprenyl-diphosphate synthase subunit 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0004497 (monooxygenase activity), GO:0016712 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0042180 (cellular ketone metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:1901661 (quinone metabolic process), GO:0042181 (ketone biosynthetic process), GO:0006743 (ubiquinone metabolic process), GO:1901663 (quinone biosynthetic process), GO:0006744 (ubiquinone biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)