Protein: NADPH--cytochrome P450 reductase (CPR) (P450R) (EC 1.6.2.4) (P36587)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000255|HAMAP-Rule:MF_03212}; Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_03212}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_03212}. Mitochondrion outer membrane {ECO:0000255|HAMAP-Rule:MF_03212}; Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_03212}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_03212}. Cell membrane {ECO:0000255|HAMAP-Rule:MF_03212}; Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_03212}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_03212}.
Sequence: MKTYEYVLLVIILILSLCYFIYNNFLNKPKAPERRVVATDSIVELMEAEKLTAAVFFGSQTGTAEDFAYRFSTEAKANFNLTNMVFDLENYDLTDLDNFDRSKLLVFFLATYGEGEPTDNAEAFLQLLEGDDTVFSSGKGIEDTPFEGIRYAIFGLGNHTYEYYNAMAKKVDAAMTRLGATRVGNLGLGDDAAGMLEEDYLQWKDDTLPEIGKLFHLQEVHKEYNPMFEVIEKPEISNTSSTVFLGEPSRQQLKGNVASKAPRSQANPFFSSPVRSLELFKSGSRNCLHLELDIADSGMRYQTGDYASICPMNPSQAVDDLLEVLGLKEKRDTVIIVKPIDTLDKAPVLSPTTYDTVFRYYYEICGIVSRQLLSFIAPFAPTPESKQELEKLGNDYDYFKKNVVDLHLNLAQVLRRVSPDAPFTKLPFSMLLENMAHMKPRYYSISSSSVVHPDKVHVTAVVDKKEWTDKNHIFYGLTTNYLLAHCRHMHGEKIPHPNGLEYTLEGPRKNWTGKIPMFVKKSTFRLAPPDVPIIMVGPGTGVAPFRGFVMERANLASKGVKVAKTLLFYGCQYSDKDFLYKEEWQQYKDVLKDSFELITAFSREQDHKIYVQHRLLEHSDTIAKLVEEGAAFYICGDADHMAKDVVNALASILTTVDVDGMKAVKALRDDNRFFEDTW
InterPro domains: - IPR023208: NADPH-cytochrome P450 reductase (family) [1-678]
- IPR023173: NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamily (homologous_superfamily) [314-435]
- IPR017938: Riboflavin synthase-like beta-barrel (homologous_superfamily) [242-524]
- IPR001094: Flavodoxin-like (domain) [54-191]
- IPR001433: Oxidoreductase FAD/NAD(P)-binding (domain) [535-646]
- IPR017927: FAD-binding domain, ferredoxin reductase-type (domain) [267-514]
- IPR001709: Flavoprotein pyridine nucleotide cytochrome reductase (domain) [301-639]
- IPR029039: Flavoprotein-like superfamily (homologous_superfamily) [26-227]
- IPR039261: Ferredoxin-NADP reductase (FNR), nucleotide-binding domain (homologous_superfamily) [524-678]
- IPR003097: Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-binding (domain) [262-481]
- IPR008254: Flavodoxin/nitric oxide synthase (domain) [53-208]
Interaction partners: - Calcium-transporting ATPase 2
- Sulfite reductase subunit beta
- Probable cytochrome b5 1
- Probable cytochrome b5 2
- Uncharacterized hydrolase C9G1.08c
- Acyl-protein thioesterase 1
- Heat shock protein 90 homolog
- N-acetyltransferase 9-like protein
- Lanosterol 14-alpha demethylase erg11
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0009055 (electron transfer activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006066 (alcohol metabolic process), GO:0044107 (cellular alcohol metabolic process), GO:0016125 (sterol metabolic process), GO:1902652 (secondary alcohol metabolic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0008204 (ergosterol metabolic process), GO:0008202 (steroid metabolic process), GO:0097384 (cellular lipid biosynthetic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:0044108 (cellular alcohol biosynthetic process), GO:0046165 (alcohol biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0016128 (phytosteroid metabolic process), GO:0006696 (ergosterol biosynthetic process), GO:0006694 (steroid biosynthetic process), GO:0016126 (sterol biosynthetic process), GO:0016129 (phytosteroid biosynthetic process), GO:1902653 (secondary alcohol biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)