Protein: Nibrin homolog (Nijmegen breakage syndrome 1 protein) (AtNbs1) (Q0H8D7)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Component of the MRE11-RAD50-NBN complex (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex may be involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, and cell cycle checkpoint control. Functions also in the very early stages of meiosis.
Subcellular location: Nucleus {ECO:0000250|UniProtKB:O60934}. Chromosome {ECO:0000250|UniProtKB:O60934}. Note=Localizes to DNA double-strand breaks (DSBs). {ECO:0000250|UniProtKB:O60934}.
Sequence: MVWGLFPVDPLSGEDKYYIFSKGIYKVGRKGCDIIINKDKGVSRIHAELTFDATTVSTSRRNKSSSDTSSFVIRVKDCSKYGTFVKTDLGTKDKVHELSNKEKILQDGDVIAFGTGSAIYRLSLIPLVFYLCPSSETFKVDQPVQDAVSSIGARISPTLSEECTHVLLEPRMQVNEALINAILAKKTIILTNWVMLLAEKSICSEIPGYSQYRPSVMVEEALVDVLELNVREKCLEGFTFVLEPTDTYRFGCSFPSLLEVCGAETVTIEDISSMSQDSQFGEINRMICVIPKSAGDKFGRFKHLSLLSRVNEMDLVCAVFSGNLPSTSLIPPSVVISSSCSTDETVVADSEAEEEETTSSVHMIDATEKAETPEKPAAIVIEDSPVTILEETSNLNEFKSVNLLADTESRGHMDEKNSSDSVTIRRDRNDEAETGKSEIIYTQDLIVRDLRSTRKVQSTGGEGVVDFKRFRKGNVTCGNSFSSLIPFAKDPYKEYDSWDVTDFMKEEKKRKQMEAIAEDLFKTEKARKRGTAGSIRGFLSGS
InterPro domains: - IPR000253: Forkhead-associated (FHA) domain (domain) [25-114]
- IPR008984: SMAD/FHA domain superfamily (homologous_superfamily) [16-130]
- IPR040227: Nibrin-related (family) [2-373]
- IPR036420: BRCT domain superfamily (homologous_superfamily) [129-211]
Interaction partners: - Double-strand break repair protein MRE11
- DNA repair protein RAD50
- Serine/threonine-protein kinase ATM
- Serine/threonine-protein kinase ATR
- Chlorophyll a-b binding protein 3, chloroplastic
- Protein BREAST CANCER SUSCEPTIBILITY 1 homolog
- Dentin sialophosphoprotein-like protein
- BRCA1-associated RING domain protein 1
- ATP-dependent DNA helicase 2 subunit KU70
- ATP-dependent DNA helicase 2 subunit KU80
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0002376 (immune system process), GO:0040007 (growth), GO:0023052 (signaling), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0048589 (developmental growth), GO:0048856 (anatomical structure development), GO:0048869 (cellular developmental process), GO:0009628 (response to abiotic stimulus), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0006955 (immune response), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0044237 (cellular metabolic process), GO:0016049 (cell growth), GO:0009607 (response to biotic stimulus), GO:0009719 (response to endogenous stimulus), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0048523 (negative regulation of cellular process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0001101 (response to acid chemical), GO:0009266 (response to temperature stimulus), GO:0009414 (response to water deprivation), GO:0044085 (cellular component biogenesis), GO:0051129 (negative regulation of cellular component organization), GO:0035556 (intracellular signal transduction), GO:0006979 (response to oxidative stress), GO:0046483 (heterocycle metabolic process), GO:0048731 (system development), GO:0006952 (defense response), GO:0071495 (cellular response to endogenous stimulus), GO:0060255 (regulation of macromolecule metabolic process), GO:1901700 (response to oxygen-containing compound), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0048229 (gametophyte development), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:0045087 (innate immune response), GO:0098542 (defense response to other organism), GO:0009408 (response to heat), GO:0099402 (plant organ development), GO:0010035 (response to inorganic substance), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0009755 (hormone-mediated signaling pathway), GO:0009888 (tissue development), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0051128 (regulation of cellular component organization), GO:0044260 (cellular macromolecule metabolic process), GO:1902074 (response to salt), GO:0048364 (root development), GO:0071229 (cellular response to acid chemical), GO:0097305 (response to alcohol), GO:0010087 (phloem or xylem histogenesis), GO:0051052 (regulation of DNA metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0071310 (cellular response to organic substance), GO:0019438 (aromatic compound biosynthetic process), GO:1902075 (cellular response to salt), GO:0048466 (androecium development), GO:0009741 (response to brassinosteroid), GO:0009651 (response to salt stress), GO:1902904 (negative regulation of supramolecular fiber organization), GO:0070542 (response to fatty acid), GO:0010468 (regulation of gene expression), GO:0009753 (response to jasmonic acid), GO:0022607 (cellular component assembly), GO:0010639 (negative regulation of organelle organization), GO:0051053 (negative regulation of DNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0033993 (response to lipid), GO:0090558 (plant epidermis development), GO:0006259 (DNA metabolic process), GO:0033043 (regulation of organelle organization), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0014070 (response to organic cyclic compound), GO:0042542 (response to hydrogen peroxide), GO:0014070 (response to organic cyclic compound), GO:1901701 (cellular response to oxygen-containing compound), GO:0000302 (response to reactive oxygen species), GO:0071407 (cellular response to organic cyclic compound), GO:0071396 (cellular response to lipid), GO:0045910 (negative regulation of DNA recombination), GO:0006260 (DNA replication), GO:0032205 (negative regulation of telomere maintenance), GO:0071215 (cellular response to abscisic acid stimulus), GO:0034614 (cellular response to reactive oxygen species), GO:0097306 (cellular response to alcohol), GO:0070301 (cellular response to hydrogen peroxide), GO:0048366 (leaf development)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)