Protein: Actin-histidine N-methyltransferase (EC 2.1.1.85) (Endothelial differentiation inhibitory protein D10) (Protein-L-histidine N-tele-methyltransferase) (SET domain-containing protein 3) (Q91WC0)
Organism: Mus musculus (Mouse)
Function: Protein-histidine N-methyltransferase that specifically mediates 3-methylhistidine (tele-methylhistidine) methylation of actin at 'His-73'. Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery. Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin.
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:Q86TU7}. Nucleus {ECO:0000269|PubMed:21832073}. Note=Localizes mainly in the cytoplasm. {ECO:0000250|UniProtKB:Q86TU7}.
Sequence: MGKKSRVKTQKSGTGATATVSPKEILNLTSELLQKCSSPAPSPGKEWEEYTQIRALVEKIRKKQKGLSVTFDGKREDYFPDLMKWASENGASVEGFEMVNFKEEGFGLRATRDIKAEELFLWVPRKLLMTVESAKNSVLGPLYSQDRILQAMGNIALAFHLLCERASPNSFWQPYIQTLPSEYDTPLYFEEEEVRCLQSTQAIHDVFSQYKNTARQYAYFYKVIQTHPHANKLPLKESFTYEDYRWAVSSVMTRQNQIPTEDGSRVTLALIPLWDMCNHTNGLITTGYNLEDDRCECVALQDFQAGDQIYIFYGTRSNAEFVIHSGFFFDNNSHDRVKIKLGVSKSDRLYAMKAEVLARAGIPTSSVFALHSTEPPISAQLLAFLRVFCMTEEELKEHLLGDSAIDRIFTLGNAEFPVSWDNEVKLWTFLEDRASLLLKTYKTTIEEDKIVLKNPDLSVRATMAIKLRLGEKEILEKAVKSAAVNREYYRKHMEERAPLPRYEESDLGLLEGGVGDSRLPLVLRKLEEEAGVQESLSLTETVSKVKAAENGLVNGENLIPNGTRSENESLSPEESENVTGEESSGSMAKVKERL
InterPro domains: - IPR036464: Rubisco LSMT, substrate-binding domain superfamily (homologous_superfamily) [329-520]
- IPR044428: SETD3, SET domain (domain) [80-329]
- IPR046341: SET domain superfamily (homologous_superfamily) [79-333]
- IPR015353: Rubisco LSMT, substrate-binding domain (domain) [344-475]
- IPR001214: SET domain (domain) [94-314]
- IPR050600: SETD3/SETD6 methyltransferase (family) [66-470]
- IPR025785: Actin-histidine N-methyltransferase SETD3 (family) [20-507]
Interaction partners: - Histone-lysine N-methyltransferase SETMAR
- Histone-lysine N-methyltransferase NSD3
- Actin, gamma-enteric smooth muscle
- Histone-lysine N-methyltransferase SMYD3
- N-lysine methyltransferase SMYD2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0008168 (methyltransferase activity), GO:0008757 (S-adenosylmethionine-dependent methyltransferase activity), GO:0008170 (N-methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0048522 (positive regulation of cellular process), GO:0032259 (methylation), GO:0019222 (regulation of metabolic process), GO:0008283 (cell population proliferation), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0071704 (organic substance metabolic process), GO:0040008 (regulation of growth), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0019538 (protein metabolic process), GO:0001558 (regulation of cell growth), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0043414 (macromolecule methylation), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0051128 (regulation of cellular component organization), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0036211 (protein modification process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0043412 (macromolecule modification), GO:0006479 (protein methylation), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0010468 (regulation of gene expression), GO:0008213 (protein alkylation), GO:0018022 (peptidyl-lysine methylation), GO:1902680 (positive regulation of RNA biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0016571 (histone methylation), GO:0018193 (peptidyl-amino acid modification), GO:0016570 (histone modification), GO:0034968 (histone lysine methylation), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0018205 (peptidyl-lysine modification)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)