Protein: SH2B adapter protein 2 (Adapter protein with pleckstrin homology and Src homology 2 domains) (SH2 and PH domain-containing adapter protein APS) (O14492)
Organism: Homo sapiens (Human)
Function: Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways. May be involved in coupling from immunoreceptor to Ras signaling. Acts as a negative regulator of cytokine signaling in collaboration with CBL. Binds to EPOR and suppresses EPO-induced STAT5 activation, possibly through a masking effect on STAT5 docking sites in EPOR. Suppresses PDGF-induced mitogenesis. May induce cytoskeletal reorganization via interaction with VAV3.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:9989826}. Cell membrane {ECO:0000269|PubMed:9989826}. Note=Cytoplasmic before PDGF stimulation. After PDGF stimulation, localized at the cell membrane and peripheral region.
Sequence: MNGAGPGPAAAAPVPVPVPVPDWRQFCELHAQAAAVDFAHKFCRFLRDNPAYDTPDAGASFSRHFAANFLDVFGEEVRRVLVAGPTTRGAAVSAEAMEPELADTSALKAAPYGHSRSSEDVSTHAATKARVRKGFSLRNMSLCVVDGVRDMWHRRASPEPDAAAAPRTAEPRDKWTRRLRLSRTLAAKVELVDIQREGALRFMVADDAAAGSGGSAQWQKCRLLLRRAVAEERFRLEFFVPPKASRPKVSIPLSAIIEVRTTMPLEMPEKDNTFVLKVENGAEYILETIDSLQKHSWVADIQGCVDPGDSEEDTELSCTRGGCLASRVASCSCELLTDAVDLPRPPETTAVGAVVTAPHSRGRDAVRESLIHVPLETFLQTLESPGGSGSDSNNTGEQGAETDPEAEPELELSDYPWFHGTLSRVKAAQLVLAGGPRNHGLFVIRQSETRPGEYVLTFNFQGKAKHLRLSLNGHGQCHVQHLWFQSVLDMLRHFHTHPIPLESGGSADITLRSYVRAQDPPPEPGPTPPAAPASPACWSDSPGQHYFSSLAAAACPPASPSDAAGASSSSASSSSAASGPAPPRPVEGQLSARSRSNSAERLLEAVAATAAEEPPEAAPGRARAVENQYSFY
InterPro domains: - IPR001849: Pleckstrin homology domain (domain) [193-308]
- IPR030523: SH2B adapter protein (family) [1-632]
- IPR035058: SH2B2, SH2 domain (domain) [409-504]
- IPR036860: SH2 domain superfamily (homologous_superfamily) [407-521]
- IPR015012: Phenylalanine zipper (domain) [22-78]
- IPR000980: SH2 domain (domain) [415-515]
- IPR036290: Phenylalanine zipper superfamily (homologous_superfamily) [21-81]
- IPR011993: PH-like domain superfamily (homologous_superfamily) [180-312]
Interaction partners: - Insulin receptor
- E3 ubiquitin-protein ligase CBL
- Growth factor receptor-bound protein 2
- High affinity nerve growth factor receptor
- BDNF/NT-3 growth factors receptor
- NT-3 growth factor receptor
- E3 ubiquitin-protein ligase CBL-B
- Insulin receptor substrate 1
- Tyrosine-protein kinase JAK2
- Mast/stem cell growth factor receptor Kit
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0060090 (molecular adaptor activity), GO:0030674 (protein-macromolecule adaptor activity), GO:0005515 (protein binding), GO:0035591 (signaling adaptor activity), GO:0005102 (signaling receptor binding), GO:0019899 (enzyme binding), GO:0019900 (kinase binding), GO:0030159 (signaling receptor complex adaptor activity), GO:0030971 (receptor tyrosine kinase binding), GO:0005068 (transmembrane receptor protein tyrosine kinase adaptor activity), GO:0019901 (protein kinase binding), GO:1990782 (protein tyrosine kinase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0048583 (regulation of response to stimulus), GO:0023051 (regulation of signaling), GO:0023056 (positive regulation of signaling), GO:0048522 (positive regulation of cellular process), GO:0048584 (positive regulation of response to stimulus), GO:0050794 (regulation of cellular process), GO:0009967 (positive regulation of signal transduction), GO:0010647 (positive regulation of cell communication), GO:0010646 (regulation of cell communication), GO:0009966 (regulation of signal transduction), GO:0090092 (regulation of transmembrane receptor protein serine/threonine kinase signaling pathway), GO:1902533 (positive regulation of intracellular signal transduction), GO:0090100 (positive regulation of transmembrane receptor protein serine/threonine kinase signaling pathway), GO:1902531 (regulation of intracellular signal transduction), GO:0060390 (regulation of SMAD protein signal transduction), GO:0060391 (positive regulation of SMAD protein signal transduction)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)