Protein: Lecithin retinol acyltransferase (EC 2.3.1.135) (Phosphatidylcholine--retinol O-acyltransferase) (Phosphatidylcholine-retinol-O-acyltransferase) (Q9JI60)
Organism: Mus musculus (Mouse)
Function: Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision (By similarity). It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments (By similarity). Required for the survival of cone photoreceptors and correct rod photoreceptor cell morphology.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:17114808, ECO:0000269|PubMed:28758396}; Single-pass membrane protein {ECO:0000269|PubMed:17114808}. Rough endoplasmic reticulum {ECO:0000250}. Endosome, multivesicular body {ECO:0000250}. Cytoplasm, perinuclear region {ECO:0000250}. Note=Present in the rough endoplasmic reticulum and multivesicular body in hepatic stellate cells. Present in the rough endoplasmic reticulum and perinuclear region in endothelial cells (By similarity). {ECO:0000250}.
Sequence: MKNPMLEAASLLLEKLLLISNFKLFSVSVPGGGTGKNRPYEISSFVRGDVLEVSRTHFIHYGIYLGENRVAHLMPDILLALTNDKERTQKVVSNKRLLLGVICKVASIRVDTVEDFAYGADILVNHLDGTLKKKSLLNEEVARRAEQQLGLTPYSLLWNNCEHFVTYCRYGSRISPQAEKFYDTVKIIIRDQRSSLASAVLGLASIVYTGLASYMTLPAICIPFCLWMMSG
InterPro domains: - IPR007053: LRAT domain (domain) [45-177]
- IPR042288: Lecithin retinol acyltransferase (family) [1-230]
Interaction partners: - Retinoid isomerohydrolase
- Retinol dehydrogenase 5
- Retinol dehydrogenase 12
- Cytochrome P450 26A1
- Retinol dehydrogenase 10
- Retinol dehydrogenase 19
- Retinol dehydrogenase 11
- Retinol dehydrogenase 16
- Retinol dehydrogenase
- Retinol dehydrogenase 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016746 (acyltransferase activity), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups), GO:0008374 (O-acyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009605 (response to external stimulus), GO:0065008 (regulation of biological quality), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0044281 (small molecule metabolic process), GO:0042445 (hormone metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0009991 (response to extracellular stimulus), GO:1901615 (organic hydroxy compound metabolic process), GO:0006629 (lipid metabolic process), GO:0007584 (response to nutrient), GO:0120254 (olefinic compound metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0006066 (alcohol metabolic process), GO:1901700 (response to oxygen-containing compound), GO:0006766 (vitamin metabolic process), GO:0010817 (regulation of hormone levels), GO:0042572 (retinol metabolic process), GO:0010033 (response to organic substance), GO:0006720 (isoprenoid metabolic process), GO:0033993 (response to lipid), GO:0031667 (response to nutrient levels), GO:0034308 (primary alcohol metabolic process), GO:0033273 (response to vitamin), GO:0006775 (fat-soluble vitamin metabolic process), GO:0032526 (response to retinoic acid), GO:0006721 (terpenoid metabolic process), GO:0033189 (response to vitamin A), GO:0016101 (diterpenoid metabolic process), GO:0001523 (retinoid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)