Protein: Helicase-like transcription factor (EC 2.3.2.27) (EC 3.6.4.-) (RING-type E3 ubiquitin transferase HLTF) (RUSH-1) (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3) (Sucrose nonfermenting protein 2-like 3) (Q95216)
Organism: Oryctolagus cuniculus (Rabbit)
Function: Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity. This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1, to which this protein can bind directly. Mediates repression by c-Rel through a DNA-looping mechanism. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA (By similarity). Transcriptional regulator that mediates the ability of prolactin to augment progesterone-dependent transcription of the SCGB1A1/uteroglobin gene through a bipartite progesterone receptor half-site/overlapping Y-box combination (-38/-26) where progesterone activation is attenuated by nuclear factor Y binding. Regulation also involves two GC-rich sequences in the proximal promoter (positions -162/+90) and a RUSH/SMARCA3 site (positions -616/-611) in the 5'-untranslated region.
Subcellular location: Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Cytoplasmic and nuclear localization in the presence of prolactin. Nuclear localization is stimulated by progesterone.
Sequence: MSWMFKRDPVWKYLQTVQYGVHGNFSRLSYPTFFPRFEFQDIIPPDDFLTSDEELDSVLFGTLRGHVVGLRYYTGVVNNNEMVALQREPNNPYDKNAIKVNNVNGNQVGYLKKELAAALAYIMDNKLAQIEGVVPYGANNAFTMPLQMTFWGKEENRKAVLDQLKKHGFKLGPAPKTLGFSLESGWGSGRAGPSYSMPVHAAIQMTTEQLKTEFDKLFEDLKEDDKTQEMEPAEAVETPLLPHQKQALAWMVSRENSRELPPFWELRNDLYYNTITNFSEKDQPENVHGGILADDMGLGKTLTAIAVILTNFHDGKPLPVERMKKNQVKKECNSSESDKPGRKDTIKKTDGLSKEGSRYSEEPSISDVKKNKYSMSELSSSQPKRKKIAVQYIESSDSEEIEISELPQKMKGKLKNVQSETKRVKVGPSKIKEDTAFACALTSSASTTTKKILKKGASAQRVQRKLMFEERPRTTLIICPLSVLSNWIDQFGQHIKSDVHLNFYVYYGPDRIRDPALLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRNPNAQQTKAVLDLEAERRWVLTGTPIQNSLKDLWSLLSFLKLKPFVDREWWHRTIQRPVTMGDEGGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERPVFIQHITLSDEERKIYQSVKSEGKATIGRYFNEGTVLAHYADVLGLLLRLRQICCHTHLLTNTVSSSGPSGNDTPEELRKKLIKKMKLILSSGSDEECAICLDSLTVPVITHCAHVFCKPCICQCIQNEQPHAKCPLCRNDIHGDNLLECPPEELACDSEKKSNMEWTSSSKINALMHALIDLRTKNPNIKSLVVSQFTTFLSLIETPLKASGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLSLKAGGVGLNLCAASRVFLMDPAWNPAAEDQRFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNTKRELAAGAFGTKKNANEMKQAKINEIRTLIDL
InterPro domains: - IPR017907: Zinc finger, RING-type, conserved site (conserved_site) [772-781]
- IPR000330: SNF2, N-terminal (domain) [243-717]
- IPR049730: SNF2/RAD5-like, C-terminal helicase domain (domain) [825-958]
- IPR038718: SNF2-like, N-terminal domain superfamily (homologous_superfamily) [206-642]
- IPR050628: SNF2/RAD54 Helicase and Transcription Factor (family) [170-1002]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [737-809]
- IPR014905: HIRAN domain (domain) [60-154]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [215-1005]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [236-611]
- IPR001650: Helicase, C-terminal domain-like (domain) [831-999]
- IPR001841: Zinc finger, RING-type (domain) [757-798]
Interaction partners: - RING-type E3 ubiquitin transferase
- Proliferating cell nuclear antigen
- DNA repair protein RAD51 homolog 1
- Protein S100-A10
- DNA polymerase zeta catalytic subunit
- Exonuclease 1
- RNA helicase
- DNA repair protein RAD52 homolog
- UBC core domain-containing protein
- UBC core domain-containing protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044238 (primary metabolic process), GO:0071704 (organic substance metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0070647 (protein modification by small protein conjugation or removal), GO:0032446 (protein modification by small protein conjugation), GO:0016567 (protein ubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140513 (nuclear protein-containing complex), GO:0005667 (transcription regulator complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0090575 (RNA polymerase II transcription regulator complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)