Protein: Probable acyl-activating enzyme 18, peroxisomal (EC 6.2.1.-) (Q84P17)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: May be involved in the peroxisomal activation of 2,4-dichlorophenoxybutyric acid (2,4-DB), a precursor of active auxins that inhibit root growth.
Subcellular location: Peroxisome {ECO:0000269|PubMed:19043666}.
Sequence: MWKSIGELSCDDYVKAGLTLEDAKEFDKLVSDVITKAIETDPRDQWKALVDESVLKPWHPHPLHQLLYYSVYSNWDSSVHGPPLYWFPSLSQSKSTNLGKLMEYHGPRLLGPSYKNPLESFELFRRFSVEHPEVYWSFVIDELSLVFHTPPRCILNKSKPEGTWLPDAVLNIAECCLMPSSHPKKEDDSVAVVWRNEGFDDSPVNRMTIKELREQVMLVANAISGSFEKGDTIAIDMPMTVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKISKAKGIFTQDYILRGGRRFPLYSRVVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNAKPHSSGENYYRPIYLPVESVINILFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGHTYCWPTNLGWVMGPTLMFSCFLTGATLALYSGSPLGRGFGKFVQDAGVTVLGTVPSLVKTWKRTNCMEGLNWTKIKFFATTGEASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSPLQPQAFGAFSTPSMTTRIIIFDENGVPYPDDQPCTGEVGLFPQHLGATDRLLNANHDEVYFKGMPMYKETRLRRHGDIVKRTVGGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECISETAAVTLTPPNGGPELLVIFAVLKEGFKQQSGEELKMKFSRTIQKDLNPLFKVSFVKIVPEFPRTASSKLLRRVLRDQIKQELLSLRSRI
InterPro domains: - IPR042099: ANL, N-terminal domain (homologous_superfamily) [105-610]
- IPR032387: Acetyl-coenzyme A synthetase, N-terminal domain (domain) [125-176]
- IPR000873: AMP-dependent synthetase/ligase domain (domain) [184-554]
- IPR045851: AMP-binding enzyme, C-terminal domain superfamily (homologous_superfamily) [611-725]
- IPR020845: AMP-binding, conserved site (conserved_site) [359-370]
Interaction partners: - At4g25315
- Similarity to Ta11-like non-LTR retroelement protein
- WAT1-related protein At5g40240
- At4g01000
- Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplastic
- Aldehyde dehydrogenase family 3 member H1
- Vacuolar protein sorting-associated protein 20 homolog 1
- ACT domain-containing protein ACR8
- Aldehyde dehydrogenase family 2 member B4, mitochondrial
- Aldehyde dehydrogenase family 2 member C4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0016877 (ligase activity, forming carbon-sulfur bonds), GO:0016405 (CoA-ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0044281 (small molecule metabolic process), GO:0009719 (response to endogenous stimulus), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901700 (response to oxygen-containing compound), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:0006631 (fatty acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:0016053 (organic acid biosynthetic process), GO:0033993 (response to lipid), GO:0008610 (lipid biosynthetic process), GO:0070542 (response to fatty acid), GO:0009753 (response to jasmonic acid), GO:0006633 (fatty acid biosynthetic process), GO:0019752 (carboxylic acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0072330 (monocarboxylic acid biosynthetic process), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0042579 (microbody), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005777 (peroxisome)