Protein: Formate dehydrogenase-O major subunit (EC 1.17.1.9) (Aerobic formate dehydrogenase major subunit) (FDH-Z subunit alpha) (Formate dehydrogenase-O subunit alpha) (P32176)
Organism: Escherichia coli (strain K12)
Function: Allows to use formate as major electron donor during aerobic respiration. Subunit alpha possibly forms the active site.
Subcellular location: Periplasm {ECO:0000305}.
Sequence: MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTRETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFIHSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLSTGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWVDIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIRSGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYDKSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCEYIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSNIQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSMMKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNKNKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENGSIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNYAIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIFAGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQLLKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFETPLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAILQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIGIPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV
InterPro domains: - IPR006963: Molybdopterin oxidoreductase, 4Fe-4S domain (domain) [43-106]
- IPR006443: Formate dehydrogenase-N, alpha subunit (family) [2-1015]
- IPR027467: Molybdopterin oxidoreductase, molybdopterin cofactor binding site (binding_site) [48-66]
- IPR006656: Molybdopterin oxidoreductase (domain) [107-589]
- IPR009010: Aspartate decarboxylase-like domain superfamily (homologous_superfamily) [836-1016]
- IPR006657: Molybdopterin dinucleotide-binding domain (domain) [894-1009]
- IPR006311: Twin-arginine translocation pathway, signal sequence (conserved_site) [1-33]
- IPR006655: Molybdopterin oxidoreductase, prokaryotic, conserved site (conserved_site) [928-955]
Interaction partners: - Formate dehydrogenase-O iron-sulfur subunit
- Formate dehydrogenase, cytochrome b556
- Protein FdhE
- Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit
- Formate dehydrogenase, nitrate-inducible, cytochrome b556
- Sulfur carrier protein FdhD
- S-formylglutathione hydrolase YeiG
- S-formylglutathione hydrolase FrmB
- Formate dehydrogenase, nitrate-inducible, major subunit
- Formyltetrahydrofolate deformylase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0005515 (protein binding), GO:0043167 (ion binding), GO:0051540 (metal cluster binding), GO:0016491 (oxidoreductase activity), GO:0009055 (electron transfer activity), GO:0016725 (oxidoreductase activity, acting on CH or CH2 groups), GO:0051536 (iron-sulfur cluster binding), GO:0043169 (cation binding), GO:0051539 (4 iron, 4 sulfur cluster binding), GO:0046872 (metal ion binding), GO:0046914 (transition metal ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006091 (generation of precursor metabolites and energy), GO:0006082 (organic acid metabolic process), GO:0015980 (energy derivation by oxidation of organic compounds), GO:0043436 (oxoacid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0045333 (cellular respiration), GO:0032787 (monocarboxylic acid metabolic process), GO:0009061 (anaerobic respiration)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:1902494 (catalytic complex), GO:0031975 (envelope), GO:0042597 (periplasmic space), GO:0016020 (membrane), GO:1990204 (oxidoreductase complex), GO:0030313 (cell envelope), GO:0030288 (outer membrane-bounded periplasmic space)