Protein: Hemicentin-2 (A2AJ76)
Organism: Mus musculus (Mouse)
Function: Not known
Subcellular location: Secreted, extracellular space, extracellular matrix {ECO:0000269|PubMed:17015624}. Cleavage furrow {ECO:0000269|PubMed:21215633}. Note=The antibody used in PubMed:17015624 and PubMed:21215633 to determine subcellular location does not distinguish between HMCN1 and HMCN2. {ECO:0000269|PubMed:17015624, ECO:0000269|PubMed:21215633}.
Sequence: MTPGAQLLPLLVAISTAVAAVVTSDAPTKTLSPATGDATLAFVFDVTGSMWDDLMQVIDGASRILERSLSSRSRVIANYALVPFHDPDIGPVTLTADPVVFQRELRELYVQGGGDCPEMSVGAIKAAVEVANPGSFIYVFSDARAKDYHKKKELLQLLQLKQSQVVFVLTGDCGDRTHPGYLVFEEIASTSSGQVFQLDKQQVSEVLKWVESAIQASKVHLLSADHEEEGEHTWRIPFDPSLKEVTIALSGPGPEIEVRDPLGRVLQTDEGLNVLLNIPDSAKVVAFKPEHPGLWAIKVYSSGRHSVRISGISNINFRAGFSMQPSLDLNHTIEWPLQGVPISLVINSTGLQAPGHLESVELSHSSGRSLLTLPTQLLSNGSTHQLWAGPPFHVPKERFYLKVKGKDHEGNPLLRVSGVSYSAVAPGVPLVSMAPKIHGYLQQPLLVSCSVYSTLPFQLQLQRDGERLGEERYFQESGNSSWEIPRASKAEEGTYQCIAVSRAGSGRASAQIVITDPPPQLVPGPNVTVSPGETAILSCQVLGETPYNLTWVRDWRALPATTGRISQLSDLSLEVRSIIPTDGGQYQCVASNPNGVTRATTWLLVREAPQVSINARSQRFSQGVEVRVSCSASGYPTPHISWSREGLALPEDSRIHVDAQGTLIIQGLAPEDAGNYSCQATNEVGTDEETVTLYYTDPPSVSAVNAVVLTAVGEEAVLLCAASGVPPPRVIWYRGGLEVILAPGDSRSGTLRIPEAQERDAGLYTCKAVNELGDASAEIQLVVGNAPRLTDPPQDVTVELGKSVFLTCRATGRPPPIVTWRRGDGQALEPGRGSRTGQRDSGVLVFERVSLEDQAPYVCEARNVFGKAQAEARLVVTGHAPPQIANSASVVRVLEGQPVSLTCVILAGRPLPERRWLKAGSPLPPGNRHAVRADGSLHLDRALQEDAGRYSCVATNVAGSQHRDVELVVQVPPRIHPTSTHHVTNEGVPASLPCIASGVPTPKITWTKETNALTTSGHYSVSRNGTLVIVQPSPQDAGAYVCTATNSVGFSSQEMWLSVNTKPMIKMNGSQAVDVPLRVTVKAGEEVTLDCEAQGSPTPLLTWTKDANPLLPVTNRYELLPSGSLRLAQAQVGDNGLYGCTASNPAGATSRRYVLRVQVPPQVQPGPRVLKVLAGEALDLNCVAEGNPQPQLNWFKDGMALMGEGAQGSVHFAAVKTSDAGLYRCEASNSAGTDTWKLELLVLEPPHWGTDETKSLLERVAGENASLPCPAQGTPKPRITWRRGPSSEPLNGRPDVAVLDEGSLFLSSVSLADSGEYECQATNEVGSASRRAKLVVYVPPSIREEGHITNVSGLAGQPLTLECDINGFPAPEVAWLKDGQLVGDSGGGWDGEEASGHRLLDGSRSLHFPRIQESHSGLYSCQAENQAGSAQRDFNLAVFIPPSLLGAGAAQEVLGLAGADVTLECQTSGVPTPQVEWTKDGQPILPGDPHILLQEDGQVLRIISSHLGDEGQYQCVAFSPAGQQAKDFQLSIHSPPTIWGSNETGEVTVLEGHTAQLLCEARGMPSPAITWYKDGTLLAPSSEVVYSKGGRQLQLVKAQPSDAGLYTCQASNPAGITKKSTSLEVYVPPTIEGADGGPYLVQAVAGRPVALECVARGHPPPTISWQHEGLPVVDSNGTWLEAGGALQLENPGEASGGLYSCVASSPAGEAVLQYSVEMQVPPQLLVAEGMGQVTATVGQSLDLPCQASGSPVPTIQWLQNGRPAEELAGVQLASQGTILHISHVELNHSGLFACQATNEAGTAGAEVEVSVHGKQVSVNLGASFSAHHWWGEPHSPFPATCNPPVCRHWSAYPKPSLVERWRGRGNLRGQPSGTVREPGLTLLSQIEKADLRDEGVYTCSATNLAGESKKDVTLKVLVPPNIEPGPVNKVVLENASVTLECLASGVPPPDVSWFKGRQPISTQRRVIVSADGRVLHIERVQLSDAGSYRCVATNVAGSAGLKYGLRVNVPPRITLPPNLPGPVLLGTPFRLTCNATGTPRPTLIWLKDGNPVSPEGIPGLKVFPGGQVLTVASARASDSGSYSCVAVSAVGEDRRDVILQVHMPPSILGEELNMSVVVNESVTLECQSHAVPPPVLRWQKDGRPLEPHPGIRLSADKALLEVDRAAVWDAGHYTCEAINQAGRSEKHFNLHVWVPPAFPSKEPYTLTVTEGQTARLSCDCQGIPFPKISWRKDGQPLPGEGDSLEQVLAVGRLLYLGQAQSAQEGTYTCECSNAAGTSSQEQSLEVLVPPQVTGLWEPLTTVSVIQDGNTTLACNATGKPLPVVTWQRDGQPVSVEPGLRLQNQNHSLHVERAQASHAGGYSCVAENTAGRAERRFALSVLAPPHLTGDSDSLTNVTATLHGSFTLLCEAAGVPAPTVQWFQEGQPISPREGTYLLAGGWMLKMTQAQEQDRGLYSCLASNEAGEARRNFSVEVLVPPSIENEDLEEVIKVPEGQTAQLECNATGHPPPKVTWFKDGQSLTVEDPYEMSPDGAFLWIPQANLSNAGHYSCIASNAVGEKTKHTQLSVLVVPTILGVPEKNANEEVTVTINNPISLICEALAFPSPNITWMKDGSPFEASKNIQLLPGTHGLQILNAQKEDAGQYTCVVTNELGEATKNYHVEVLIPPSISKDDPLGEVSVKEVKTKVNSSLTLECECWATPPPSISWYKDGRPVTPSHRLSVLGEGRLLQIQPTQVSDSGRYLCVATNVAGEDDQDFNVLIQVPPMFQKMGDVDAGFEPLPHEEEAQGRVTEYREIVENNPAYLYCDTNAIPPPELTWYREGQPLSAADGVSVLQGGRILQLPLVQAEDAGRYSCKAANEVGEDWLHYELLVLTPPVIPGDTQELVEEVTVNASSAVSLECPALGNPAPAVSWFQNGLPVSPSPRLQVLEEGQVLKVATAEVADAASYMCVAENQAGSAEKLFTLKVQVPPQISDWTTSQLTATLNSSVSLPCEVYAHPNPEVTWYKDGQPLSLGQEAFLLPGTHTLRLARAQPADSGTYLCEALNAAGRDQKMVQLNVLVPPSFKQAPGGPQEAIQVRAGDKAILSCETDSLPEPAVTWFKDQQPLALGQRIQGLQGGQTLEILDSQASDKGVYSCKVSNTAGEAIRTFVLAIQVPPTFEKPERETVNQVAGRTLVLACDVSGIPAPTVTWLKDRLPVESSVVHGVVSRGGRLQLSHLQPAQAGTYTCVAENAQAEARKDFVVSVLVPPQIQDSGMAQEHNVLEKQEIRLHCEAEGQPPPDITWLKDGGLLDQHVGPHLRFYLDGSTLVLKGLRTADSGAYTCVAHNPAGEDARLHTVNVLVPPTIKQQAGDTGTLVSRTGELVTMVCPVQGSPPIHVSWLKDGLPLPLSQRTLLHSSGRTLRISQVQLADSGVFTCVAASPAGVADRNFTLLVLVPPILEPVEFQNNVMAAQGSEVVLPCEARGSPLPLVSWMKDGEPLLPQSLEQGPGLKLESVSVGDAGTYSCTAASEAGEARRHFQLTVMDPPHIEESGETSELSLTPGAHLELLCEARGIPPPNITWHKDGQALRRTENDSQAGRVLRVDNAGLYTCLAESPAGEVEKSFRVRVQAPPNVVGPRGPRSVVGLAPGQLILECSVEAEPAPEIEWHRGGVLLQADAHTHFPEQGRFLKLQALSTADGGDYSCTARNRAGSTSVAFRVEIHTAPTIQSGPNTVNVSVNRTTLLPCQTHGVPTPLVSWRKDGIPLHPGSPRLEFLPEGSLRIHPVLAQDAGHYLCLASNSAGSDRKGLDLRVFEPPAIAPGPSNLTLTAYSPASLPCEARGSPKPLVTWWKDGQKLDLRLQQGAYRLLPSNALFLTAPSPQDSAQFECVVSNEVGESRRRYQVTVHVPPTIADDQTHFTVTRMAPVILTCHSTGSPTPAVSWSKAGTQLGARGSGYRILPSGALEIERALPLHAGRYTCTARNSAGVARKHMVLTVQASPVVKPLPSVVQVVASEEVLLPCEASGIPQPMVIWQKEGLSIPEGAHMQVLPSGQLRIMHASPEDAGNYFCIAQNSVGSAMAKTRLVVQVPPVIENGLPDLSTIEGSHALLPCTAKGSPEPAITWEKDGHLVSGAEGKFTLQPSGELLVKNSEGQDAGTYICTAENAVGRARRRVHLTILTLPVLTTLPGDRSLRLGDRLWLRCVARGSPTPRIGWTINDQPVTEGVSEQDGGSTLQRAAVTREDSGTYTCWAENRVGRVQAVSFVHVKEAPVLQGEAFSYLVEPVGGSIQLHCVVRGDPAPDIHWTKDGLPLPISRLHFQLQNGSLTILRTKMDDAGRYQCLAVNEMGTVKKVVTVVLQSAPVFQVEPQDVTVRSGVDVELRCRATGEPVPTIEWLRAGRPLQAGRKLRALPDGSLWLEHVEAGDAGVYECVAHNHLGSVTAKALLAVRGEPRGSRGSMTGVINGQEFGMATLNISVLQQGSSEAPTIWSSISQVPASVGPLMRVLVVTIAPIYWALARESGEALNGYSLTGGSFQQESQMEFSTGELLTMTQVARGLDPDGLLLVDMKINGMIPESLADGDLRVQDFQEHYVQTGPGQLFAGSTQRFLHDSLPASLRCNHSIQYDETRGLQPQLVQHLRASSISSAFDPEAEALNFQLTTALQTEENEVGCPEGFEPDVQGAFCVDKDECSGGPSPCSHTCRNAPGHFSCSCPTGFSLAWDHRNCRDVDECAGNTHLCQEEQRCVNLLGSYNCLASCRPGFRVTADGSNCEDVDECLEQLDECHYNQLCENTPGGHHCGCPRGYRQQGHSLPCLDINECLQLPTPCVYQCQNLQGSYRCLCPPGQTLLRDGRTCIPLERNRQNITIVSHRSPFGPWLRSRVPRPSSSYHTWVSLRPGSGALNSVGRAWCPPGFIRQDGVCADLDECRVRSLCQHACQNTEGSYYCLCPSGYRLLPSGKNCQDINECEEDGIECGPGQMCFNTRGSFQCVDTPCPTTYRQGSSPGTCFRRCSQDCSASGPSTLQYRLLPLPLGVRAHHDVARLAAFSEAGIPANRTELTVLEPDPRSPFALRQLRAGQGAVYTRRALTRAGLYRLTVRAAAPRHQSVYILLIAVSPYPY
InterPro domains: - IPR050958: Cell Adhesion and Cytoskeletal Organization (family) [3622-3891]
- IPR056475: Hemicentin/VWA7, galactose-binding domain-like (domain) [217-314]
- IPR000742: EGF-like domain (domain) [4668-4993]
- IPR013106: Immunoglobulin V-set domain (domain) [534-3326]
- IPR036179: Immunoglobulin-like domain superfamily (homologous_superfamily) [431-4433]
- IPR001881: EGF-like calcium-binding domain (domain) [4668-4989]
- IPR018097: EGF-like calcium-binding, conserved site (conserved_site) [4709-4970]
- IPR013783: Immunoglobulin-like fold (homologous_superfamily) [435-4394]
- IPR007110: Immunoglobulin-like domain (domain) [426-4428]
- IPR049883: NOTCH1 EGF-like calcium-binding domain (domain) [4668-4836]
- IPR009030: Growth factor receptor cysteine-rich domain superfamily (homologous_superfamily) [4720-4996]
- IPR006605: G2 nidogen/fibulin G2F (domain) [4429-4655]
- IPR026823: Complement Clr-like EGF domain (domain) [4924-4947]
- IPR036465: von Willebrand factor A-like domain superfamily (homologous_superfamily) [39-217]
- IPR009017: Green fluorescent protein (homologous_superfamily) [4412-4646]
- IPR013098: Immunoglobulin I-set (domain) [609-4429]
- IPR000152: EGF-type aspartate/asparagine hydroxylation site (ptm) [4683-4929]
- IPR003599: Immunoglobulin domain subtype (domain) [434-4430]
- IPR003598: Immunoglobulin subtype 2 (domain) [440-4419]
- IPR056861: Hemicentin-1-like, von Willebrand factor A domain (domain) [40-200]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009605 (response to external stimulus), GO:0071840 (cellular component organization or biogenesis), GO:0006950 (response to stress), GO:0042594 (response to starvation), GO:0009991 (response to extracellular stimulus), GO:0016043 (cellular component organization), GO:0031668 (cellular response to extracellular stimulus), GO:0071496 (cellular response to external stimulus), GO:0045229 (external encapsulating structure organization), GO:0043062 (extracellular structure organization), GO:0031667 (response to nutrient levels), GO:0030198 (extracellular matrix organization), GO:0071711 (basement membrane organization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0030312 (external encapsulating structure), GO:0031012 (extracellular matrix), GO:0062023 (collagen-containing extracellular matrix)