Protein: Mitotic spindle checkpoint protein MAD2 (Mitotic arrest deficient protein 2) (Q9LU93)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. It inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
Subcellular location: Nucleus {ECO:0000269|PubMed:19710914, ECO:0000269|PubMed:22457071}. Nucleus envelope {ECO:0000269|PubMed:22457071}. Chromosome {ECO:0000269|PubMed:19710914}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:19710914}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:19710914}. Cytoplasm {ECO:0000269|PubMed:19710914, ECO:0000269|PubMed:22457071}. Note=Cytoplasmic in interphase cells. Accumulates onto both kinetochores and the spindle microtubules in cell arrested in metaphase. Present in chromocenters. Associates with unattached kinetochores upon spindle assembly checkpoint (SAC) activation. The nucleus envelope association requires the presence of NUA. {ECO:0000269|PubMed:22457071}.
Sequence: MASKTAAAKDIITLHGSAAIVSEFFCYAANSILYNRAVYPEESFVKVKKYGLPMLLIEDESVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREKSDKEIMREIQAIMRQVASSVTYLPCLDETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQMVKLHGFDTKIHKVDTLVSYKNDEWDEEE
InterPro domains: - IPR003511: HORMA domain (domain) [15-198]
- IPR045091: Mad2-like (family) [8-202]
- IPR036570: HORMA domain superfamily (homologous_superfamily) [2-204]
Interaction partners: - Anaphase-promoting complex subunit 6
- Mitotic spindle checkpoint protein MAD1
- Anaphase-promoting complex subunit 4
- Mitotic spindle checkpoint protein BUBR1
- Anaphase-promoting complex subunit 8
- Mitotic checkpoint serine/threonine-protein kinase BUB1
- Anaphase-promoting complex subunit 1
- Mitotic checkpoint protein BUB3.1
- Anaphase-promoting complex subunit 10
- Serine/threonine-protein kinase MPS1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0023052 (signaling), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:2000241 (regulation of reproductive process), GO:0051321 (meiotic cell cycle), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:2000242 (negative regulation of reproductive process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:1903046 (meiotic cell cycle process), GO:0048523 (negative regulation of cellular process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:1905132 (regulation of meiotic chromosome separation), GO:1903047 (mitotic cell cycle process), GO:0051129 (negative regulation of cellular component organization), GO:1901993 (regulation of meiotic cell cycle phase transition), GO:0035556 (intracellular signal transduction), GO:0070192 (chromosome organization involved in meiotic cell cycle), GO:0045132 (meiotic chromosome segregation), GO:0010948 (negative regulation of cell cycle process), GO:0051726 (regulation of cell cycle), GO:0007059 (chromosome segregation), GO:0061983 (meiosis II cell cycle process), GO:0140013 (meiotic nuclear division), GO:0051445 (regulation of meiotic cell cycle), GO:0033313 (meiotic cell cycle checkpoint signaling), GO:0045786 (negative regulation of cell cycle), GO:0009790 (embryo development), GO:0051447 (negative regulation of meiotic cell cycle), GO:0051128 (regulation of cellular component organization), GO:0010564 (regulation of cell cycle process), GO:0000278 (mitotic cell cycle), GO:0098813 (nuclear chromosome segregation), GO:0007346 (regulation of mitotic cell cycle), GO:0007088 (regulation of mitotic nuclear division), GO:0000075 (cell cycle checkpoint signaling), GO:0045839 (negative regulation of mitotic nuclear division), GO:0007135 (meiosis II), GO:1905818 (regulation of chromosome separation), GO:0007093 (mitotic cell cycle checkpoint signaling), GO:0045835 (negative regulation of meiotic nuclear division), GO:1902102 (regulation of metaphase/anaphase transition of meiotic cell cycle), GO:0010639 (negative regulation of organelle organization), GO:0051983 (regulation of chromosome segregation), GO:1901987 (regulation of cell cycle phase transition), GO:0045930 (negative regulation of mitotic cell cycle), GO:0040020 (regulation of meiotic nuclear division), GO:1905819 (negative regulation of chromosome separation), GO:0033043 (regulation of organelle organization), GO:0051985 (negative regulation of chromosome segregation), GO:1901988 (negative regulation of cell cycle phase transition), GO:0051783 (regulation of nuclear division), GO:0051784 (negative regulation of nuclear division), GO:0010965 (regulation of mitotic sister chromatid separation), GO:0033045 (regulation of sister chromatid segregation), GO:1901991 (negative regulation of mitotic cell cycle phase transition), GO:2000816 (negative regulation of mitotic sister chromatid separation), GO:0033046 (negative regulation of sister chromatid segregation), GO:1902099 (regulation of metaphase/anaphase transition of cell cycle), GO:0033048 (negative regulation of mitotic sister chromatid segregation), GO:0031577 (spindle checkpoint signaling), GO:0045841 (negative regulation of mitotic metaphase/anaphase transition), GO:0033047 (regulation of mitotic sister chromatid segregation), GO:1901990 (regulation of mitotic cell cycle phase transition), GO:1902100 (negative regulation of metaphase/anaphase transition of cell cycle), GO:2001251 (negative regulation of chromosome organization), GO:0033044 (regulation of chromosome organization), GO:0071174 (mitotic spindle checkpoint signaling), GO:0007094 (mitotic spindle assembly checkpoint signaling), GO:0071173 (spindle assembly checkpoint signaling), GO:0030071 (regulation of mitotic metaphase/anaphase transition)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0031974 (membrane-enclosed lumen), GO:0034399 (nuclear periphery), GO:0012505 (endomembrane system), GO:0031967 (organelle envelope), GO:0043233 (organelle lumen), GO:0005635 (nuclear envelope), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)