Protein: RNA-directed RNA polymerase L (Protein L) (EC 2.7.7.48) (Large structural protein) (RdRp) (Replicase) (Transcriptase) [Includes: cap-snatching endonuclease (EC 3.1.-.-)] (P23456)
Organism: Hantaan virus (strain 76-118) (Korean hemorrhagic fever virus)
Function: RNA-dependent RNA polymerase, which is responsible for the replication and transcription of the viral RNA genome using antigenomic RNA as an intermediate (By similarity). During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, which involves the endonuclease activity cleaving the host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription. Cleaves ssRNA substrates but not DNA (By similarity). Seems to downregulate the expression of its own and heterologous mRNAs through its endonuclease activity (By similarity).
Subcellular location: Host cytoplasm, host perinuclear region {ECO:0000250|UniProtKB:Q9YQR5}.
Sequence: MDKYREIHNKLKEFSPGTLTAVECIDYLDRLYAVRHDIVDQMIKHDWSDNKDSEEAIGKVLLFAGVPSNIITALEKKIIPNHPTGKSLKAFFKMTPDNYKISGTTIEFVEVTVTADVDKGIREKKLKYEAGLTYIEQELHKFFLKGEIPQPYKITFNVVAVRTDGSNITTQWPSRRNDGVVQYMRLVQAEISYVREHLIKTEERAALEAMFNLKFNISTHKSQPYYIPDYKGMEPIGANIEDLVDYSKDWLSRARNFSFFEVKGTAVFECFNSNEANHCQRYPMSRKPRNFLLIQCSLITSYKPATTLSDQIDSRRACSYILNLIPDTPASYLIHDMAYRYINLTREDMINYYAPRIQFKQTQNVREPGTFKLTSSMLRAESKAMLDLLNNHKSGEKHGAQIESLNIASHIVQSESVSLITKILSDLELNITEPSTQEYSTTKHTYVDTVLDKFFQNETQKYLIDVLKKTTAWHIGHLIRDITESLIAHSGLKRSKYWSLHSYNNGNVILFILPSKSLEVAGSFIRFITVFRIGPGLVDKDNLDTILIDGDSQWGVSKVMSIDLNRLLALNIAFEKALIATATWFQYYTEDQGQFPLQYAIRSVFANHFLLAICQKMKLCAIFDNLRYLIPAVTSLYSGFPSLIEKLFERPFKSSLEVYIYYNIKSLLVALAQNNKARFYSKVKLLGLTVDQSTVGASGVYPSFMSRIVYKHYRSLISEVTTCFFLFEKGLHGNMNEEAKIHLETVEWALKFREKEEKYGESLVENGYMMWELRANAELAEQQLYCQDAIELAAIELNKVLATKSSVVANSILSKNWEEPYFSQTRNISLKGMSGQVQEDGHLSSSVTIIEAIRYLSNSRHNPSLLKLYEETREQKAMARIVRKYQRTEADRGFFITTLPTRCRLEIIEDYYDAIAKNISEEYISYGGEKKILAIQGALEKALRWASGESFIELSNHKFIRMKRKLMYVSADATKWSPGDNSAKFRRFTSMLHNGLPNNKLKNCVIDALKQVYKTDFFMSRKLRNYIDSMESLDPHIKQFLDFFPDGHHGEVKGNWLQGNLNKCSSLFGVAMSLLFKQVWTNLFPELDCFFEFAHHSDDALFIYGYLEPVDDGTDWFLFVSQQIQAGHLHWFSVNTEMWKSMFNLHEHILLLGSIKISPKKTTVSPTNAEFLSTFFEGCAVSIPFVKILLGSLSDLPGLGYFDDLAAAQSRCVKALDLGASPQVAQLAVALCTSKVERLYGTAPGMVNHPAAYLQVKHTDTPIPLGGNGAMSIMELATAGIGMSDKNLLKRALLGYSHKRQKSMLYILGLFKFLMKLSDETFQHERLGQFSFIGKVQWKIFTPKSEFEFADMYTSKFLELWSSQHVTYDYIIPKGRDNLLIYLVRKLNDPSIVTAMTMQSPLQLRFRMQAKQHMKVCRLDGEWVTFREVLAAANSFAENYSATSQDMDLFQTLTSCTFSKEYAWKDFLNGIHCDVIPTKQVQRAKVARTFTVREKDQIIQNSIPAVIGYKFAVTVEEMSDVLDTAKFPDSLSVDLKTMKDGVYRELGLDISLPDVMKRIAPMLYKSSKSRVVIVQGNVEGTAEAICRYWLKSMSLVKTIRVKPHKEVLQAVSIFNRKEDIGQQKDLAALKLCIEVWRWCKANSAPYRDWFQALWFEDKTFSEWLDRFCRVGVPPIDPEIQCAALMIADIKGDYSVLQLQANRRAYSGKQYDAYCVQTYNEVTKLYEGDLRVTFNFGLDCARLEIFWDKKAYILETSITQKHVLKIMMDEVSKELIKCGMRFNTEQVQGVRHMVLFKTESGFEWGKPNIPCIVYKNCVLRTSLRTTQAINHKFMITIKDDGLRAIAQHDEDSPRFLLAHAFHTIRDIRYQAVDAVSNVWFIHKGVKLYLNPIISSGLLENFMKNLPAAIPPAAYSLIMNRAKISVDLFMFNDLLKLINPRNTLDLSGLETTGDEFSTVSSMSSRLWSEEMSLVDDDEELDDEFTIDLQDVDFENIDIEADIEHFLQDESSYTGDLLISTEETESKKMRGIVKILEPVRLIKSWVSRGLSIEKVYSPVNIILMSRYISKTFNLSTKQVSLLDPYDLTELESIVRGWGECVIDQFESLDREAQNMVVNKGICPEDVIPDSLFSFRHTMVLLRRLFPQDSISSFY
InterPro domains: - IPR048006: RNA endonuclease, cap-snatching, bunyaviral (domain) [94-164]
- IPR016268: RNA-directed RNA polymerase, hantavirus (family) [1-2151]
- IPR007322: RNA-dependent RNA polymerase, bunyaviral (domain) [562-1285]
- IPR054155: Cap-snatching endonuclease, N-terminal (domain) [3-196]
- IPR007099: RNA-directed RNA polymerase, negative-strand RNA virus (domain) [956-1142]
- IPR024378: RNA-directed RNA polymerase, hantavirus, N-terminal (domain) [198-238]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0016032 (viral process), GO:0019080 (viral gene expression), GO:0019083 (viral transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:1990904 (ribonucleoprotein complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:1902494 (catalytic complex), GO:0140513 (nuclear protein-containing complex), GO:0005737 (cytoplasm), GO:0140535 (intracellular protein-containing complex), GO:0030880 (RNA polymerase complex), GO:1990234 (transferase complex), GO:0043229 (intracellular organelle), GO:1905348 (endonuclease complex), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0061695 (transferase complex, transferring phosphorus-containing groups), GO:0005634 (nucleus)