Protein: Charged multivesicular body protein 5 (Vacuolar protein-sorting-associated protein 60) (Q9VVI9)
Organism: Drosophila melanogaster (Fruit fly)
Function: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and are delivered to lysosomes enabling degradation of membrane proteins (By similarity). Specifically down-regulates Notch signaling activity in the germarium, probably by facilitating Notch endocytosis.
Subcellular location: Endosome membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
Sequence: MNRLFGRGKPKEPGPSLNDCIAGVDARATNIEEKISNLEAELRKYREQMSKMREGPAKNSVKQKALRVLKQKKAYEQQAESLRNQSFNMEQANYAAQSLKDTQATVAAMKDGVKQMKTEYKKINIDQIEDIQDDMADMFEQADEVQEALGRTYGMPEVDDDDLQAELDALGDEIALDDDTSYLDDVVKAPEAPSREPGADSIVPGKSTIETDEFGLPKIPTSLKTT
InterPro domains: - IPR005024: Snf7 family (family) [3-220]
Interaction partners: - Vacuolar protein sorting-associated protein VTA1 homolog
- RH72336p
- GH14561p
- IST1 homolog
- Charged multivesicular body protein 4
- Vacuolar protein sorting 20, isoform A
- Charged multivesicular body protein 1, isoform A
- Vacuolar protein sorting-associated protein 28 homolog
- Tumor suppressor protein 101
- vesicle-fusing ATPase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0051234 (establishment of localization), GO:0023051 (regulation of signaling), GO:0071840 (cellular component organization or biogenesis), GO:0046907 (intracellular transport), GO:0001919 (regulation of receptor recycling), GO:0006810 (transport), GO:0060255 (regulation of macromolecule metabolic process), GO:0051649 (establishment of localization in cell), GO:0031323 (regulation of cellular metabolic process), GO:0016043 (cellular component organization), GO:0016197 (endosomal transport), GO:0016192 (vesicle-mediated transport), GO:0006996 (organelle organization), GO:0007034 (vacuolar transport), GO:0061024 (membrane organization), GO:0032509 (endosome transport via multivesicular body sorting pathway), GO:0007033 (vacuole organization), GO:0006906 (vesicle fusion), GO:0007041 (lysosomal transport), GO:0016050 (vesicle organization), GO:0048284 (organelle fusion), GO:0061025 (membrane fusion), GO:0071985 (multivesicular body sorting pathway), GO:0008333 (endosome to lysosome transport), GO:0080171 (lytic vacuole organization), GO:0090174 (organelle membrane fusion), GO:0007040 (lysosome organization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043227 (membrane-bounded organelle), GO:0031982 (vesicle), GO:0005770 (late endosome), GO:0043231 (intracellular membrane-bounded organelle), GO:0005771 (multivesicular body), GO:0097708 (intracellular vesicle)