Protein: cGMP-specific 3',5'-cyclic phosphodiesterase (EC 3.1.4.35) (DmPDE5/6) (DmPDE6) (Q9VFI9)
Organism: Drosophila melanogaster (Fruit fly)
Function: Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Has cAMP phosphodiesterase activity in vitro but not in vivo. Has a role regulating cGMP transport in Malpighian tubule principal cells.
Subcellular location: Cell membrane {ECO:0000269|PubMed:16232123, ECO:0000269|PubMed:18503409}; Lipid-anchor {ECO:0000269|PubMed:16232123, ECO:0000269|PubMed:18503409}; Cytoplasmic side {ECO:0000269|PubMed:16232123, ECO:0000269|PubMed:18503409}. Note=Expressed on the apical side of the Malpighian tubule principal cell polarized membrane. Loss of prenylation causes protein location to the cytoplasm.
Sequence: MTDVSSPAGGAASPVEMSTTSSSSAATTSASSSKPLTNGANKTAISTAAGGVTPGAVPGPGSGAIPASSSSGNQVKLEHHHRQSNNNRPAVTNRSSETKLMTPTGSSSSPSQSPSQTQASIQTQTSQQDRLAKASTTASQQDVDEVARLFEEKPEAFEKWLTERAPPEALSRLQEFIENRKPHKRPSVTSDLFQQWMAASPTVQQKSPRSLSNSSASSLPECRRHLMDLDEGELFMELIRDVANELDIDVLCHKILVNVGLLTHADRGSLFLAKGTPTNKYLVAKLFDVTQKTALKDAVTRASAEEIIIPFGIGIAGMVAQTKQMINIKEAYKDARFNCEIDLKTGYKTNAILCMPICNYEGDIIGVAQIINKTNGCMEFDEHDVEIFRRYLTFCGIGIQNAQLFEMSVQEYRRNQILLNLARSIFEEQNNLECLVTKIMTEARELLKCERCSVFLVDLDCCEASHLEKIIEKPNQPATRAIKSADSFEEKKMRNRFTVLFELGGEYQAANVSRPSVSELSSSTLAQIAQFVATTGQTVNICDVIEWVRDHNQIRAEDEIDSTQAILCMPIMNAQKKVIGVAQLINKANGVPFTDSDASIFEAFAIFCGLGIHNTQMYENACKLMAKQKVALECLSYHATASQDQTEKLTQDVIAEAESYNLYSFTFTDFELVDDDTCRAVLRMFMQCNLVSQFQIPYDVLCRWVLSVRKNYRPVKYHNWRHALNVAQTMFAMLKTGKMERFMTDLEILGLLVACLCHDLDHRGTNNAFQTKTESPLAILYTTSTMEHHHFDQCVMILNSEGNNIFQALSPEDYRSVMKTVESAILSTDLAMYFKKRNAFLELVENGEFDWQGEEKKDLLCGMMMTACDVSAIAKPWEVQHKVAKLVADEFFDQGDLEKLQLNTQPVAMMDRERKDELPKMQVGFIDVICLPLYRVLCDTFPWITPLYEGTLENRRNWQDLAEKVEMGLTWIDHDTIDKPVEEFAACADEEIKDIEFTVTTLNCNQQSQHGSEDSHTPEHQRSGSRLSMKKTGALGKAVRSKLSKTLYNSMDGSKPKTSLKLLESHVSEDMDDKSPTSPSQPQASGSMGRMSASSSTSSAGGQMVDKSKKRSKLCALL
InterPro domains: - IPR029016: GAF-like domain superfamily (homologous_superfamily) [228-629]
- IPR003607: HD/PDEase domain (domain) [715-892]
- IPR023088: 3'5'-cyclic nucleotide phosphodiesterase (family) [713-898]
- IPR003018: GAF domain (domain) [247-622]
- IPR023174: 3'5'-cyclic nucleotide phosphodiesterase, conserved site (conserved_site) [758-769]
- IPR036971: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain superfamily (homologous_superfamily) [638-965]
- IPR002073: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain (domain) [642-965]
Interaction partners: - Apyrase
- Guanylate kinase
- nucleoside diphosphate phosphatase
- Adenine phosphoribosyltransferase
- Inosine triphosphate pyrophosphatase
- GMP synthase
- Receptor-type guanylate cyclase Gyc76C
- Probable cGMP 3',5'-cyclic phosphodiesterase subunit delta
- Soluble guanylate cyclase 88E
- Guanylate cyclase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0042578 (phosphoric ester hydrolase activity), GO:0008081 (phosphoric diester hydrolase activity), GO:0004112 (cyclic-nucleotide phosphodiesterase activity), GO:0004114 (3',5'-cyclic-nucleotide phosphodiesterase activity), GO:0004115 (3',5'-cyclic-AMP phosphodiesterase activity), GO:0047555 (3',5'-cyclic-GMP phosphodiesterase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0048519 (negative regulation of biological process), GO:0032501 (multicellular organismal process), GO:0009605 (response to external stimulus), GO:0009628 (response to abiotic stimulus), GO:0023057 (negative regulation of signaling), GO:0008340 (determination of adult lifespan), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0009893 (positive regulation of metabolic process), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0006950 (response to stress), GO:0051606 (detection of stimulus), GO:0048523 (negative regulation of cellular process), GO:0048585 (negative regulation of response to stimulus), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0009581 (detection of external stimulus), GO:0009968 (negative regulation of signal transduction), GO:0010648 (negative regulation of cell communication), GO:0010035 (response to inorganic substance), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0006979 (response to oxidative stress), GO:0007635 (chemosensory behavior), GO:0009314 (response to radiation), GO:0010646 (regulation of cell communication), GO:0009966 (regulation of signal transduction), GO:0060255 (regulation of macromolecule metabolic process), GO:0009582 (detection of abiotic stimulus), GO:1901700 (response to oxygen-containing compound), GO:0009268 (response to pH), GO:0007602 (phototransduction), GO:0010033 (response to organic substance), GO:0007602 (phototransduction), GO:0009416 (response to light stimulus), GO:0010628 (positive regulation of gene expression), GO:0010446 (response to alkaline pH), GO:1902532 (negative regulation of intracellular signal transduction), GO:0042542 (response to hydrogen peroxide), GO:0000302 (response to reactive oxygen species), GO:1902531 (regulation of intracellular signal transduction), GO:0010468 (regulation of gene expression), GO:0009583 (detection of light stimulus), GO:0010752 (regulation of cGMP-mediated signaling)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)