Protein: Solute carrier family 12 member 5 (Electroneutral potassium-chloride cotransporter 2) (Furosemide-sensitive K-Cl cotransporter) (K-Cl cotransporter 2) (rKCC2) (Neuronal K-Cl cotransporter) (Q63633)
Organism: Rattus norvegicus (Rat)
Function: Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation.
Subcellular location: Cell membrane {ECO:0000250|UniProtKB:Q91V14}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q91V14}. Cell projection, dendrite {ECO:0000250|UniProtKB:Q91V14}. Note=Detected on dendrites, but not on axons of spinal cord neurons and at GPHN-positive inhibitory synapses. {ECO:0000250|UniProtKB:Q91V14}.
Sequence: MSRRFTVTSLPPAASAASADPESRRHSVADPRRLPREDVKGDGNPKESSPFINSTDTEKGREYDGRNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRLLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERRGMPSVGLADGTPVDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLDNHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETACDNEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSAAQKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVNKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEQLDRVMLVRGGGREVITIYS
InterPro domains: - IPR000076: K/Cl co-transporter (family) [46-577]
- IPR004842: SLC12A transporter family (family) [45-1139]
- IPR004841: Amino acid permease/ SLC12A domain (domain) [126-698]
- IPR018491: SLC12A transporter, C-terminal (domain) [712-1139]
Interaction partners: - STE20/SPS1-related proline-alanine-rich protein kinase
- Gamma-aminobutyric acid receptor subunit gamma-2
- Serine/threonine-protein kinase WNK3
- Neuropilin and tolloid-like protein 2
- BDNF/NT-3 growth factors receptor
- Glutamate decarboxylase 1
- Neurotrophic factor BDNF precursor form
- Glutamate decarboxylase 2
- Vesicular inhibitory amino acid transporter
- Serine/threonine-protein kinase OSR1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity), GO:1901702 (salt transmembrane transporter activity), GO:0015075 (monoatomic ion transmembrane transporter activity), GO:0015318 (inorganic molecular entity transmembrane transporter activity), GO:0022804 (active transmembrane transporter activity), GO:0008509 (monoatomic anion transmembrane transporter activity), GO:0015079 (potassium ion transmembrane transporter activity), GO:0022890 (inorganic cation transmembrane transporter activity), GO:0015103 (inorganic anion transmembrane transporter activity), GO:0015108 (chloride transmembrane transporter activity), GO:0022853 (active monoatomic ion transmembrane transporter activity), GO:0008324 (monoatomic cation transmembrane transporter activity), GO:0015291 (secondary active transmembrane transporter activity), GO:0015296 (monoatomic anion:monoatomic cation symporter activity), GO:0015293 (symporter activity), GO:0015379 (potassium:chloride symporter activity), GO:0015294 (solute:monoatomic cation symporter activity), GO:0046873 (metal ion transmembrane transporter activity), GO:0015377 (chloride:monoatomic cation symporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0040007 (growth), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0051179 (localization), GO:0050896 (response to stimulus), GO:0051234 (establishment of localization), GO:0007267 (cell-cell signaling), GO:0009628 (response to abiotic stimulus), GO:0042221 (response to chemical), GO:0055085 (transmembrane transport), GO:0007154 (cell communication), GO:0001101 (response to acid chemical), GO:1901700 (response to oxygen-containing compound), GO:0099536 (synaptic signaling), GO:0034220 (monoatomic ion transmembrane transport), GO:1901698 (response to nitrogen compound), GO:0010033 (response to organic substance), GO:0098660 (inorganic ion transmembrane transport), GO:0006810 (transport), GO:1902074 (response to salt), GO:0098655 (monoatomic cation transmembrane transport), GO:0043200 (response to amino acid), GO:0010243 (response to organonitrogen compound), GO:0006811 (monoatomic ion transport), GO:0015698 (inorganic anion transport), GO:0099537 (trans-synaptic signaling), GO:0006821 (chloride transport), GO:0098916 (anterograde trans-synaptic signaling), GO:0006820 (monoatomic anion transport), GO:0007268 (chemical synaptic transmission)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0036477 (somatodendritic compartment), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0042995 (cell projection), GO:0044297 (cell body), GO:0043025 (neuronal cell body), GO:0120025 (plasma membrane bounded cell projection), GO:0005886 (plasma membrane), GO:0043005 (neuron projection)