Protein: CD2-associated protein (Mesenchyme-to-epithelium transition protein with SH3 domains 1) (METS-1) (Q9JLQ0)
Organism: Mus musculus (Mouse)
Function: Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton (By similarity). In collaboration with CBLC, modulates the rate of RET turnover and may act as regulatory checkpoint that limits the potency of GDNF on neuronal survival. Controls CBLC function, converting it from an inhibitor to a promoter of RET degradation (By similarity). May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis. Plays a role in epithelial cell junctions formation (By similarity).
Subcellular location: Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q9Y5K6}. Cell projection, ruffle {ECO:0000250|UniProtKB:Q9Y5K6}. Cell junction {ECO:0000250|UniProtKB:Q9Y5K6}. Note=Colocalizes with F-actin and BCAR1/p130Cas in membrane ruffles (By similarity). Located at podocyte slit diaphragm between podocyte foot processes (PubMed:10514378, PubMed:11733379). During late anaphase and telophase, concentrates in the vicinity of the midzone microtubules and in the midbody in late telophase (By similarity). {ECO:0000250|UniProtKB:Q9Y5K6, ECO:0000269|PubMed:10514378, ECO:0000269|PubMed:11733379}.
Sequence: MVDYIVEYDYDAVHDDELTIRVGEIIRNVKKLQEEGWLEGELNGRRGMFPDNFVKEIKRETEPKDDNLPIKRERQGNVASLVQRISTYGLPAGGIQPHPQTKAIKKKTKKRQCKVLFDYSPQNEDELELIVGDVIDVIEEVEEGWWSGTLNNKLGLFPSNFVKELESTEDGETHNAQEESEVPLTGPTSPLPSPGNGSEPAPGSVAQPKKIRGIGFGDIFKEGSVKLRTRTSSSETEEKKTEKPLILQPLGSRTQNVEVTKPDVDGKIKAKEYCRTLFPYTGTNEDELTFREGEIIHLISKETGEAGWWKGELNGKEGVFPDNFAVQISELDKDFPKPKKPPPPAKGPAPKPDLSAAEKKAFPLKAEEKDEKSLLEQKPSKPAAPQVPPKKPTAPTKASNLLRSPGAVYPKRPEKPVPPPPPAAKINGEVSIISSKIDTEPVSKPKLDPEQLPVRPKSVDLDAFVARNSKETDDVNFDDIASSENLLHLTANRPKMPGRRLPGRFNGGHSPTQSPEKTLKLPKEDDSGNLKPLEFKKDASYSSKSSLSTPSSASKVNTAAFLTPLELKAKAEADDGKRNSVDELRAQIIELLCIVDALKKDHGKELEKLRKELEEEKAMRSNLEVEIAKLKKAVLLS
InterPro domains: - IPR035775: CD2-associated protein, first SH3 domain (domain) [3-58]
- IPR036028: SH3-like domain superfamily (homologous_superfamily) [4-351]
- IPR035776: CD2-associated protein, second SH3 domain (domain) [111-165]
- IPR050384: Endophilin/SH3RF (family) [59-326]
- IPR035777: CD2-associated protein, third SH3 domain (domain) [271-327]
- IPR001452: SH3 domain (domain) [1-330]
Interaction partners: - Nephrin
- Podocin
- Tight junction protein 1
- E3 ubiquitin-protein ligase CBL
- Synaptopodin
- Ras and Rab interactor 3
- Alpha-actinin-4
- Kin of IRRE-like protein 3
- Cadherin-3
- F-actin-uncapping protein LRRC16A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0044877 (protein-containing complex binding), GO:0005515 (protein binding), GO:0008013 (beta-catenin binding), GO:0050839 (cell adhesion molecule binding), GO:0005102 (signaling receptor binding), GO:0045296 (cadherin binding), GO:0005126 (cytokine receptor binding), GO:0070851 (growth factor receptor binding), GO:0005172 (vascular endothelial growth factor receptor binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0023057 (negative regulation of signaling), GO:0048870 (cell motility), GO:0032879 (regulation of localization), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0023051 (regulation of signaling), GO:0071840 (cellular component organization or biogenesis), GO:0030029 (actin filament-based process), GO:0048523 (negative regulation of cellular process), GO:0051050 (positive regulation of transport), GO:0048585 (negative regulation of response to stimulus), GO:0048522 (positive regulation of cellular process), GO:0009968 (negative regulation of signal transduction), GO:0010648 (negative regulation of cell communication), GO:1903530 (regulation of secretion by cell), GO:1903829 (positive regulation of protein localization), GO:0044085 (cellular component biogenesis), GO:0051049 (regulation of transport), GO:0051047 (positive regulation of secretion), GO:0010646 (regulation of cell communication), GO:0060341 (regulation of cellular localization), GO:0009966 (regulation of signal transduction), GO:0016043 (cellular component organization), GO:0051222 (positive regulation of protein transport), GO:0030036 (actin cytoskeleton organization), GO:0016477 (cell migration), GO:1903532 (positive regulation of secretion by cell), GO:0006929 (substrate-dependent cell migration), GO:0030030 (cell projection organization), GO:0051223 (regulation of protein transport), GO:1904951 (positive regulation of establishment of protein localization), GO:0070201 (regulation of establishment of protein localization), GO:0051046 (regulation of secretion), GO:0032880 (regulation of protein localization), GO:1902532 (negative regulation of intracellular signal transduction), GO:0043933 (protein-containing complex organization), GO:0006996 (organelle organization), GO:0050708 (regulation of protein secretion), GO:0050714 (positive regulation of protein secretion), GO:1902531 (regulation of intracellular signal transduction), GO:0097435 (supramolecular fiber organization), GO:0007015 (actin filament organization), GO:0022607 (cellular component assembly), GO:0007010 (cytoskeleton organization), GO:0051058 (negative regulation of small GTPase mediated signal transduction), GO:0051056 (regulation of small GTPase mediated signal transduction), GO:0065003 (protein-containing complex assembly), GO:0030031 (cell projection assembly), GO:0120036 (plasma membrane bounded cell projection organization), GO:0120031 (plasma membrane bounded cell projection assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0031252 (cell leading edge), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0098590 (plasma membrane region), GO:0120025 (plasma membrane bounded cell projection), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0001726 (ruffle), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0030139 (endocytic vesicle), GO:0031253 (cell projection membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0097708 (intracellular vesicle)