Protein: Purine nucleoside phosphorylase YfiH (P33644)
Organism: Escherichia coli (strain K12)
Function: Purine nucleoside enzyme that catalyzes the phosphorolysis of adenosine and inosine nucleosides, yielding D-ribose 1-phosphate and the respective free bases, adenine and hypoxanthine. Also catalyzes the phosphorolysis of S-methyl-5'-thioadenosine into adenine and S-methyl-5-thio-alpha-D-ribose 1-phosphate. Also has adenosine deaminase activity. May also act as a polyphenol oxidase: able to oxidize syringaldazine and 2,2'-azino-bis(3-ethylbenzthiazoline-6- sulfonic acid) (ABTS) in vitro
Sequence: MSKLIVPQWPQPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNPDHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAVMTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPRAFEVGGEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTYTENETFFSYRRDKTTGRMASFIWLI
InterPro domains: - IPR011324: Cytotoxic necrotizing factor-like, catalytic (homologous_superfamily) [4-242]
- IPR003730: Multi-copper polyphenol oxidoreductase (family) [4-242]
- IPR038371: Multi-copper polyphenol oxidoreductase superfamily (homologous_superfamily) [1-243]
Interaction partners: - Ribosomal large subunit pseudouridine synthase D
- Pyridoxal phosphate homeostasis protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016787 (hydrolase activity), GO:0043167 (ion binding), GO:0005515 (protein binding), GO:0016491 (oxidoreductase activity), GO:0016757 (glycosyltransferase activity), GO:0016810 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds), GO:0016679 (oxidoreductase activity, acting on diphenols and related substances as donors), GO:0046983 (protein dimerization activity), GO:0042802 (identical protein binding), GO:0043169 (cation binding), GO:0016763 (pentosyltransferase activity), GO:0019239 (deaminase activity), GO:0042803 (protein homodimerization activity), GO:0016682 (oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor), GO:0046872 (metal ion binding), GO:0016814 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines), GO:0046914 (transition metal ion binding), GO:0004000 (adenosine deaminase activity), GO:0005507 (copper ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006520 (amino acid metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044282 (small molecule catabolic process), GO:1901575 (organic substance catabolic process), GO:0006082 (organic acid metabolic process), GO:0044248 (cellular catabolic process), GO:0016054 (organic acid catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0043436 (oxoacid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0009063 (amino acid catabolic process), GO:0019752 (carboxylic acid metabolic process), GO:1901606 (alpha-amino acid catabolic process), GO:0046395 (carboxylic acid catabolic process), GO:0009069 (serine family amino acid metabolic process), GO:0009071 (serine family amino acid catabolic process), GO:0006544 (glycine metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)