Protein: DDB1- and CUL4-associated factor 12 homolog (Q9VGE3)
Organism: Drosophila melanogaster (Fruit fly)
Function: Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation. Required for synaptic function and plasticity at larval neuromuscular junctions (NMJs) by promoting neurotransmitter release. The DCX(DCAF12) complex catalyzes ubiquitination and degradation of yorkie (yki), thereby regulating the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. Acts as a regulator of tissue growth during development: required for the apoptotic elimination of supernumerary cells during metamorphosis.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:30670470}. Nucleus {ECO:0000269|PubMed:30670470}. Presynapse {ECO:0000269|PubMed:30670470}. Note=Mainly localizes to presynapses in larval neuromuscular junctions. {ECO:0000269|PubMed:30670470}.
Sequence: MFNGMVRTIRDSVVGTYPSCHVSSRLEERRAKQRAMRQERRRKPDKPDDFVTYEDSGSDEETPQQQEEVLNTSYNLCDYLRSRESGLRDRRSVNVEYTSRYVLTHDMLRETQISLGYINKVFCSKWLSSRQVVFGTKCNKLLVYDVNMRRVDAIPTLSNSRANHPEVQGGLHAIELSPSRSFLATGARNSSDIAVYRLPTLDPVCVGEGGHRDLIMGMCWLDDQFLVSGSKDSRMALWRINEDHMEFPDGGEEACPTFATIHPLSVKEVRTAQRIRSLCFNKEFKEIAALSLNGYIHIFNAETFKQTLSRKLPNCQDNVSIAYHSDGLYAVGCRSYTILLDARTLQTIKKITSRYSGCGIRATSFEGNLLTVGTGLGMLLFYDIRAGKYLESSVNASRTVALKCSKGIVYPEDEMDGFQQVKYVPAIYTHCYDSTRMRLFAAGGPLPATLVGNYAGVWQ
InterPro domains: - IPR001680: WD40 repeat (repeat) [157-300]
- IPR015943: WD40/YVTN repeat-like-containing domain superfamily (homologous_superfamily) [71-409]
- IPR056151: DDB1- and CUL4-associated factor 12, beta-propeller domain (domain) [106-458]
- IPR051191: DDB1-CUL4-associated factor 12 (family) [36-459]
- IPR036322: WD40-repeat-containing domain superfamily (homologous_superfamily) [115-393]
Interaction partners: - DNA damage-binding protein 1
- Cullin-4A
- Uncharacterized protein
- WD repeat-containing protein 37
- Uncharacterized protein, isoform A
- RING-box protein 1A
- F-box and WD repeat domain containing 5
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0019222 (regulation of metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0009056 (catabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0080090 (regulation of primary metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0044248 (cellular catabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0009057 (macromolecule catabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0051052 (regulation of DNA metabolic process), GO:0044265 (cellular macromolecule catabolic process), GO:0030163 (protein catabolic process), GO:0006275 (regulation of DNA replication), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0010498 (proteasomal protein catabolic process), GO:0051603 (proteolysis involved in protein catabolic process), GO:0019941 (modification-dependent protein catabolic process), GO:0043161 (proteasome-mediated ubiquitin-dependent protein catabolic process), GO:0006511 (ubiquitin-dependent protein catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005815 (microtubule organizing center), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0140535 (intracellular protein-containing complex), GO:1990234 (transferase complex), GO:0000151 (ubiquitin ligase complex), GO:0005813 (centrosome), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0031461 (cullin-RING ubiquitin ligase complex), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0080008 (Cul4-RING E3 ubiquitin ligase complex), GO:0015630 (microtubule cytoskeleton)