Protein: U3 small nucleolar RNA-associated protein 25 (U3 snoRNA-associated protein 25) (U three protein 25) (O74974)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: DEAD-box RNA helicase-like protein required for pre-18S rRNA processing, specifically at sites A0, A1, and A2.
Subcellular location: Nucleus, nucleolus {ECO:0000269|PubMed:16823372}.
Sequence: MAIESDLDGASMDELAGKSYEALLYLMNKNKKRKPEKKDDKEKSSKKVQKKNKVIESLNEMEVEMEDENENENDEQEAIQYYINVESQDADETLHQEAQDSSEDPFHQHFDYDDSEVIKNSIAAYDEGKLCKSIEESKLGVSQSFFPDVTKRLPCSCLSEIKNIDDLGLKQRILDSYKKQKPSGQLTSMQIELAKAMFNYQDVFFTNMSMKSHKLGTSLLALHALNHVFKTRDRVLKNSARISQNPELEFRDQGYTRPKVLILLPTRNSAFEFINLLISYSGTNQVENRKRFDNEFSIEKEVISEKKPEDFRYLFSGNTDDMFRLGVKFTRKTVKLFSQFYNSDIIVASPLGLRLAIGNKGDKKRDLDYLSSIEIAMVDQAHALVMQNWEHIECIFDELNGLPKEAHGCDFSRVRPWYLDQQARYMRQTILFSQYNNLDINSFFSHYMSNIAGKVKFRSLLHGVLNRLGYKVTQTFVRISVDSIIKVPDARFSYFTGSILVQLKKYSQSGILVYIPSYFDFVRVRNYMDEEGINYSAISEYSSVSDMTRARNLFYQGRTNIMLYTERAHHFRRFDFRGVKAVVMYGPPTNPQFYVELVRMPMRTISEGNLDSDAAKCRIMYTKFDSICLEGIVGYQRVSNMCHGKHEVFEFL
InterPro domains: - IPR053940: UTP25, NTP hydrolase-like domain (domain) [200-455]
- IPR053939: UTP25, C-terminal domain (domain) [465-651]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [147-624]
- IPR010678: U3 small nucleolar RNA-associated protein 25 (family) [33-651]
Interaction partners: - Uncharacterized protein C3B8.09
- U3 small nucleolar ribonucleoprotein protein mpp10
- U3 small nucleolar RNA-associated protein 22
- U3 small nucleolar RNA-associated protein 21 homolog
- Uncharacterized protein C18B11.06
- U3 small nucleolar ribonucleoprotein protein imp4
- rRNA biogenesis protein rrp36
- Probable ATP-dependent RNA helicase prh1
- Pre-rRNA-processing protein esf2
- rRNA-processing protein fcf1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0003676 (nucleic acid binding), GO:0140098 (catalytic activity, acting on RNA), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0004518 (nuclease activity), GO:0003723 (RNA binding), GO:0004540 (ribonuclease activity), GO:0019843 (rRNA binding), GO:0030515 (snoRNA binding), GO:0004519 (endonuclease activity), GO:0016891 (RNA endonuclease activity, producing 5'-phosphomonoesters), GO:0016893 (endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters), GO:0001046 (core promoter sequence-specific DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0010467 (gene expression), GO:0042254 (ribosome biogenesis), GO:0042274 (ribosomal small subunit biogenesis), GO:0016070 (RNA metabolic process), GO:0090305 (nucleic acid phosphodiester bond hydrolysis), GO:0006396 (RNA processing), GO:0090501 (RNA phosphodiester bond hydrolysis), GO:0034470 (ncRNA processing), GO:0034660 (ncRNA metabolic process), GO:0030490 (maturation of SSU-rRNA), GO:0006364 (rRNA processing), GO:0000460 (maturation of 5.8S rRNA), GO:0000469 (cleavage involved in rRNA processing), GO:0090502 (RNA phosphodiester bond hydrolysis, endonucleolytic), GO:0016072 (rRNA metabolic process), GO:0000462 (maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)), GO:0000478 (endonucleolytic cleavage involved in rRNA processing), GO:0000447 (endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)), GO:0000466 (maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)), GO:0000479 (endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA))
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus)