Protein: Fructose-6-phosphate aldolase 1 (P78055)
Organism: Escherichia coli (strain K12)
Function: Catalyzes the reversible formation of fructose 6-phosphate from dihydroxyacetone (DHA) and D-glyceraldehyde 3-phosphate via an aldolization reaction. Can utilize several aldehydes as acceptor compounds in vitro, and hydroxyacetone (HA) or 1-hydroxy-butan-2-one as alternative donor substrate. Is also able to catalyze the direct stereoselective self-aldol addition of glycolaldehyde to furnish D-(-)- threose, and cross-aldol reactions of glycolaldehyde to other aldehyde acceptors. Is not able to cleave fructose, fructose 1-phosphate, glucose 6-phosphate, sedoheptulose 1,7-bisphosphate, xylulose 5- phosphate, ribulose 5-phosphate, and fructose 1,6-bisphosphate; cannot use dihydroxyacetone phosphate as donor compound nor D-glyceraldehyde as acceptor. Does not display transaldolase activity
Subcellular location: Cytoplasm
Sequence: MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLPQLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAEGIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQAKVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRTSI
InterPro domains: - IPR004731: Transaldolase type 3B/Fructose-6-phosphate aldolase (family) [1-214]
- IPR018225: Transaldolase, active site (active_site) [24-99]
- IPR033919: Transaldolase/Fructose-6-phosphate aldolase, archaeal/bacterial (family) [2-213]
- IPR001585: Transaldolase/Fructose-6-phosphate aldolase (family) [3-212]
- IPR013785: Aldolase-type TIM barrel (homologous_superfamily) [1-220]
- IPR023001: Fructose-6-phosphate aldolase (family) [1-220]
Interaction partners: - Arabinose 5-phosphate isomerase KdsD
- Probable dehydratase YbiW
- Putative pyruvate formate-lyase 3-activating enzyme
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016829 (lyase activity), GO:0016830 (carbon-carbon lyase activity), GO:0016832 (aldehyde-lyase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006066 (alcohol metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006766 (vitamin metabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006767 (water-soluble vitamin metabolic process), GO:0009110 (vitamin biosynthetic process), GO:0044272 (sulfur compound biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0034308 (primary alcohol metabolic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0042723 (thiamine-containing compound metabolic process), GO:0072527 (pyrimidine-containing compound metabolic process), GO:0046165 (alcohol biosynthetic process), GO:0042364 (water-soluble vitamin biosynthetic process), GO:0006772 (thiamine metabolic process), GO:0034309 (primary alcohol biosynthetic process), GO:0042724 (thiamine-containing compound biosynthetic process), GO:0072528 (pyrimidine-containing compound biosynthetic process), GO:0009228 (thiamine biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)