Protein: Neutrophil cytosol factor 4 (NCF-4) (Neutrophil NADPH oxidase factor 4) (SH3 and PX domain-containing protein 4) (p40-phox) (p40phox) (Q15080)
Organism: Homo sapiens (Human)
Function: Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:8280052}. Endosome membrane {ECO:0000269|PubMed:11684018}; Peripheral membrane protein {ECO:0000269|PubMed:11684018}; Cytoplasmic side {ECO:0000269|PubMed:11684018}. Membrane {ECO:0000269|PubMed:11684018, ECO:0000269|PubMed:8280052}; Peripheral membrane protein {ECO:0000269|PubMed:11684018}. Note=Translocates to the membrane upon activation by phorbol myristate acetate (PMA). {ECO:0000269|PubMed:8280052}.
Sequence: MAVAQQLRAESDFEQLPDDVAISANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFHALQSKLEERFGPDSKSSALACTLPTLPAKVYVGVKQEIAEMRIPALNAYMKSLLSLPVWVLMDEDVRIFFYQSPYDSEQVPQALRRLRPRTRKVKSVSPQGNSVDRMAAPRAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGIFPLSFVKILKDFPEEDDPTNWLRCYYYEDTISTIKDIAVEEDLSSTPLLKDLLELTRREFQREDIALNYRDAEGDLVRLLSDEDVALMVRQARGLPSQKRLFPWKLHITQKDNYRVYNTMP
InterPro domains: - IPR034912: Neutrophil cytosol factor 4, PX domain (domain) [19-141]
- IPR036028: SH3-like domain superfamily (homologous_superfamily) [170-231]
- IPR035541: Neutrophil cytosol factor P40, SH3 domain (domain) [174-227]
- IPR001452: SH3 domain (domain) [170-229]
- IPR034853: Neutrophil cytosol factor P40, PB1 domain (domain) [238-329]
- IPR053793: PB1-like domain (domain) [237-329]
- IPR000919: Neutrophil cytosol factor P40 (family) [56-308]
- IPR001683: Phox homology (domain) [18-140]
- IPR000270: PB1 domain (domain) [237-329]
- IPR051228: NADPH Oxidase and PX Domain-Containing (family) [12-332]
- IPR036871: PX domain superfamily (homologous_superfamily) [2-144]
Interaction partners: - Neutrophil cytosol factor 2
- Cytochrome b-245 light chain
- Ras-related C3 botulinum toxin substrate 2
- NADPH oxidase 2
- Neutrophil cytosol factor 1
- NADPH oxidase 1
- NADPH oxidase activator 1
- NADPH oxidase organizer 1
- RAC-alpha serine/threonine-protein kinase
- NADPH oxidase 4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0032501 (multicellular organismal process), GO:0042592 (homeostatic process), GO:0051241 (negative regulation of multicellular organismal process), GO:0051239 (regulation of multicellular organismal process), GO:0044237 (cellular metabolic process), GO:0048871 (multicellular organismal-level homeostasis), GO:0019222 (regulation of metabolic process), GO:0009892 (negative regulation of metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0001818 (negative regulation of cytokine production), GO:0001817 (regulation of cytokine production), GO:0097009 (energy homeostasis), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0001659 (temperature homeostasis), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression), GO:0032715 (negative regulation of interleukin-6 production)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)