Protein: Calsequestrin-1 (Calmitine) (Calsequestrin, skeletal muscle isoform) (O09165)
Organism: Mus musculus (Mouse)
Function: Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca(2+) ions (By similarity). Regulates the release of lumenal Ca(2+) via the calcium release channel RYR1; this plays an important role in triggering muscle contraction. Negatively regulates store-operated Ca(2+) entry (SOCE) activity (By similarity).
Subcellular location: Endoplasmic reticulum {ECO:0000250|UniProtKB:P31415}. Sarcoplasmic reticulum {ECO:0000250|UniProtKB:P31415}. Sarcoplasmic reticulum lumen {ECO:0000269|PubMed:22049211, ECO:0000269|PubMed:7945294}. Sarcoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side {ECO:0000250|UniProtKB:P07221}. Mitochondrion matrix {ECO:0000269|PubMed:7945294}. Note=This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of fast skeletal muscle cells (PubMed:22049211). Preferentially forms linear and round aggregates in the endoplasmic reticulum (ER) of resting cells. In a minority of cells, homogeneously detected in the ER lumen. Colocalizes with STIM1 at endoplasmic reticulum in response to a depletion of intracellular calcium (By similarity). {ECO:0000250|UniProtKB:P31415, ECO:0000269|PubMed:22049211}.
Sequence: MRATDRMGARAVSELRLALLFVLVLGTPRLGVQGEDGLDFPEYDGVDRVINVNAKNYKNVFKKYEVLALLYHEPPEDDKASQRQFEMEELILELAAQVLEDKGVGFGLVDSEKDAAVAKKLGLTEEDSVYVFKGDEVIEYDGEFSADTLVEFLLDVLEDPVELIEGERELQAFENIEDEIKLIGYFKSKDSEHYKAYEDAAEEFHPYIPFFATFDSKVAKKLTLKLNEIDFYEAFMEEPMTIPDKPNSEEEIVSFVEEHRRSTLRKLKPESMYETWEDDLDGIHIVAFAEEADPDGYEFLETLKAVAQDNTENPDLSIIWIDPDDFPLLVPYWEKTFDIDLSAPQIGVVNVTDADSIWMEMDNEEDLPSADELEDWLEDVLEGEINTEDDDDDDDDDDDDDDDDD
InterPro domains: - IPR036249: Thioredoxin-like superfamily (homologous_superfamily) [39-381]
- IPR001393: Calsequestrin (family) [12-396]
- IPR041858: Calsequestrin, middle TRX-fold domain (domain) [160-261]
- IPR041859: Calsequestrin, N-terminal TRX-fold domain (domain) [39-158]
- IPR041860: Calsequestrin, C-terminal TRX-fold domain (domain) [262-381]
- IPR018233: Calsequestrin, conserved site (conserved_site) [35-391]
Interaction partners: - Triadin
- Aspartyl/asparaginyl beta-hydroxylase
- Ryanodine receptor 1
- Ryanodine receptor 2
- Ryanodine receptor 3
- Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
- Calsequestrin-2
- Sarcalumenin
- Voltage-dependent L-type calcium channel subunit alpha-1S
- Alpha-actinin-3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0043167 (ion binding), GO:0043169 (cation binding), GO:0046872 (metal ion binding), GO:0005509 (calcium ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0009653 (anatomical structure morphogenesis), GO:0051051 (negative regulation of transport), GO:0032879 (regulation of localization), GO:0060306 (regulation of membrane repolarization), GO:0071840 (cellular component organization or biogenesis), GO:0051239 (regulation of multicellular organismal process), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0065008 (regulation of biological quality), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0065009 (regulation of molecular function), GO:0030029 (actin filament-based process), GO:0048523 (negative regulation of cellular process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0032989 (cellular component morphogenesis), GO:0051049 (regulation of transport), GO:0044085 (cellular component biogenesis), GO:0035556 (intracellular signal transduction), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0051282 (regulation of sequestering of calcium ion), GO:0032409 (regulation of transporter activity), GO:0044092 (negative regulation of molecular function), GO:0044057 (regulation of system process), GO:0034762 (regulation of transmembrane transport), GO:0030036 (actin cytoskeleton organization), GO:0034763 (negative regulation of transmembrane transport), GO:0061061 (muscle structure development), GO:0002027 (regulation of heart rate), GO:0032410 (negative regulation of transporter activity), GO:0042391 (regulation of membrane potential), GO:0043271 (negative regulation of monoatomic ion transport), GO:0016043 (cellular component organization), GO:0010927 (cellular component assembly involved in morphogenesis), GO:0034765 (regulation of monoatomic ion transmembrane transport), GO:0055001 (muscle cell development), GO:0090257 (regulation of muscle system process), GO:0030239 (myofibril assembly), GO:0043267 (negative regulation of potassium ion transport), GO:1903522 (regulation of blood circulation), GO:0019932 (second-messenger-mediated signaling), GO:0022607 (cellular component assembly), GO:0032413 (negative regulation of ion transmembrane transporter activity), GO:0034766 (negative regulation of monoatomic ion transmembrane transport), GO:0042692 (muscle cell differentiation), GO:0022898 (regulation of transmembrane transporter activity), GO:0043269 (regulation of monoatomic ion transport), GO:0006996 (organelle organization), GO:0031032 (actomyosin structure organization), GO:0097435 (supramolecular fiber organization), GO:0051279 (regulation of release of sequestered calcium ion into cytosol), GO:0070925 (organelle assembly), GO:0006937 (regulation of muscle contraction), GO:0010880 (regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum), GO:0032412 (regulation of monoatomic ion transmembrane transporter activity), GO:1904063 (negative regulation of cation transmembrane transport), GO:0010959 (regulation of metal ion transport), GO:0019722 (calcium-mediated signaling), GO:0045214 (sarcomere organization), GO:0007010 (cytoskeleton organization), GO:0008016 (regulation of heart contraction), GO:0051146 (striated muscle cell differentiation), GO:1901017 (negative regulation of potassium ion transmembrane transporter activity), GO:1901380 (negative regulation of potassium ion transmembrane transport), GO:1904062 (regulation of monoatomic cation transmembrane transport), GO:0055002 (striated muscle cell development), GO:1901016 (regulation of potassium ion transmembrane transporter activity), GO:0010881 (regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion), GO:0055117 (regulation of cardiac muscle contraction), GO:1901379 (regulation of potassium ion transmembrane transport), GO:0006942 (regulation of striated muscle contraction), GO:0043266 (regulation of potassium ion transport), GO:0051924 (regulation of calcium ion transport), GO:0010882 (regulation of cardiac muscle contraction by calcium ion signaling), GO:0140694 (non-membrane-bounded organelle assembly), GO:1903169 (regulation of calcium ion transmembrane transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0071944 (cell periphery), GO:0098796 (membrane protein complex), GO:0031984 (organelle subcompartment), GO:1990351 (transporter complex), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0043229 (intracellular organelle), GO:0016528 (sarcoplasm), GO:0098827 (endoplasmic reticulum subcompartment), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0043233 (organelle lumen), GO:0005783 (endoplasmic reticulum), GO:1902495 (transmembrane transporter complex), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0016529 (sarcoplasmic reticulum), GO:0005788 (endoplasmic reticulum lumen), GO:0043231 (intracellular membrane-bounded organelle), GO:0034702 (ion channel complex), GO:0098588 (bounding membrane of organelle), GO:0005789 (endoplasmic reticulum membrane), GO:0070013 (intracellular organelle lumen), GO:0033017 (sarcoplasmic reticulum membrane), GO:0034703 (cation channel complex), GO:0034704 (calcium channel complex)