Protein: CD177 antigen (Human neutrophil alloantigen 2a) (HNA-2a) (NB1 glycoprotein) (NB1 GP) (Polycythemia rubra vera protein 1) (PRV-1) (CD antigen CD177) (Q8N6Q3)
Organism: Homo sapiens (Human)
Function: In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production. In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration. Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro. However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo. Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils. By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis.
Subcellular location: Cell membrane {ECO:0000269|PubMed:12239154, ECO:0000269|PubMed:12675722, ECO:0000269|PubMed:17244676, ECO:0000269|PubMed:17580308, ECO:0000269|PubMed:18462208, ECO:0000269|PubMed:21193407, ECO:0000269|PubMed:24926686, ECO:0000269|PubMed:27227454, ECO:0000269|PubMed:28240246, ECO:0000269|PubMed:28807980}; Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:12239154, ECO:0000269|PubMed:17244676}. Membrane raft {ECO:0000269|PubMed:21193407}; Lipid-anchor, GPI-like-anchor {ECO:0000269|PubMed:12239154, ECO:0000269|PubMed:17244676}. Secreted {ECO:0000269|PubMed:12239154, ECO:0000269|PubMed:18462208}. Cytoplasmic granule membrane {ECO:0000269|PubMed:18462208}. Cell projection, lamellipodium {ECO:0000269|PubMed:28807980}. Note=Cell surface expression on neutrophils is increased upon TNF-alpha, fMLP or CXCL8/IL8-mediated stimulation (PubMed:17244676, PubMed:17580308). In neutrophils, stored predominantly in secondary and tertiary granules (PubMed:18462208). Can also be shedded from the cell membrane (PubMed:12239154, PubMed:18462208). Localizes to lamellar protrusions in spreading neutrophils (PubMed:28807980). {ECO:0000269|PubMed:12239154, ECO:0000269|PubMed:17244676, ECO:0000269|PubMed:17580308, ECO:0000269|PubMed:18462208, ECO:0000269|PubMed:28807980}.
Sequence: MSAVLLLALLGFILPLPGVQALLCQFGTVQHVWKVSDLPRQWTPKNTSCDSGLGCQDTLMLIESGPQVSLVLSKGCTEAKDQEPRVTEHRMGPGLSLISYTFVCRQEDFCNNLVNSLPLWAPQPPADPGSLRCPVCLSMEGCLEGTTEEICPKGTTHCYDGLLRLRGGGIFSNLRVQGCMPQPGCNLLNGTQEIGPVGMTENCNRKDFLTCHRGTTIMTHGNLAQEPTDWTTSNTEMCEVGQVCQETLLLLDVGLTSTLVGTKGCSTVGAQNSQKTTIHSAPPGVLVASYTHFCSSDLCNSASSSSVLLNSLPPQAAPVPGDRQCPTCVQPLGTCSSGSPRMTCPRGATHCYDGYIHLSGGGLSTKMSIQGCVAQPSSFLLNHTRQIGIFSAREKRDVQPPASQHEGGGAEGLESLTWGVGLALAPALWWGVVCPSC
InterPro domains: - IPR045860: Snake toxin-like superfamily (homologous_superfamily) [131-380]
- IPR016054: Ly-6 antigen/uPA receptor-like (domain) [48-395]
- IPR051899: Fertilization and immune response mediator protein (family) [1-436]
Interaction partners: - Platelet endothelial cell adhesion molecule
- Myeloblastin
- Contactin-5
- Contactin-6
- Integrin alpha-M
- Urokinase plasminogen activator surface receptor
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0019222 (regulation of metabolic process), GO:0051240 (positive regulation of multicellular organismal process), GO:0032879 (regulation of localization), GO:0002684 (positive regulation of immune system process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0002682 (regulation of immune system process), GO:0051239 (regulation of multicellular organismal process), GO:0051050 (positive regulation of transport), GO:0048522 (positive regulation of cellular process), GO:0002696 (positive regulation of leukocyte activation), GO:0051049 (regulation of transport), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0051282 (regulation of sequestering of calcium ion), GO:0043270 (positive regulation of monoatomic ion transport), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0034762 (regulation of transmembrane transport), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0034764 (positive regulation of transmembrane transport), GO:0002694 (regulation of leukocyte activation), GO:0080090 (regulation of primary metabolic process), GO:0050865 (regulation of cell activation), GO:0050867 (positive regulation of cell activation), GO:0034767 (positive regulation of monoatomic ion transmembrane transport), GO:0010562 (positive regulation of phosphorus metabolic process), GO:0051246 (regulation of protein metabolic process), GO:0051928 (positive regulation of calcium ion transport), GO:0034765 (regulation of monoatomic ion transmembrane transport), GO:0051247 (positive regulation of protein metabolic process), GO:0043269 (regulation of monoatomic ion transport), GO:0051174 (regulation of phosphorus metabolic process), GO:0051279 (regulation of release of sequestered calcium ion into cytosol), GO:1904427 (positive regulation of calcium ion transmembrane transport), GO:0051281 (positive regulation of release of sequestered calcium ion into cytosol), GO:0031399 (regulation of protein modification process), GO:1904062 (regulation of monoatomic cation transmembrane transport), GO:1904064 (positive regulation of cation transmembrane transport), GO:0045937 (positive regulation of phosphate metabolic process), GO:0031401 (positive regulation of protein modification process), GO:0010959 (regulation of metal ion transport), GO:0019220 (regulation of phosphate metabolic process), GO:0042325 (regulation of phosphorylation), GO:0001932 (regulation of protein phosphorylation), GO:0051924 (regulation of calcium ion transport), GO:0042327 (positive regulation of phosphorylation), GO:1903169 (regulation of calcium ion transmembrane transport), GO:0001934 (positive regulation of protein phosphorylation), GO:0050730 (regulation of peptidyl-tyrosine phosphorylation), GO:0050731 (positive regulation of peptidyl-tyrosine phosphorylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005576 (extracellular region), GO:0005886 (plasma membrane)