Protein: Respiratory nitrate reductase 2 beta chain (EC 1.7.5.1) (P19318)
Organism: Escherichia coli (strain K12)
Function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit.
Subcellular location: Cell membrane; Peripheral membrane protein.
Sequence: MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGNKMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE
InterPro domains: - IPR029263: Respiratory nitrate reductase beta, C-terminal (domain) [358-436]
- IPR006547: Nitrate reductase, beta subunit (family) [1-493]
- IPR038262: Nitrate reductase beta, C-terminal domain superfamily (homologous_superfamily) [362-435]
- IPR017896: 4Fe-4S ferredoxin-type, iron-sulphur binding domain (domain) [7-271]
Interaction partners: - Respiratory nitrate reductase 2 alpha chain
- Respiratory nitrate reductase 1 alpha chain
- Respiratory nitrate reductase 2 gamma chain
- Probable nitrate reductase molybdenum cofactor assembly chaperone NarW
- Nitrate/nitrite transporter NarU
- Respiratory nitrate reductase 1 gamma chain
- Respiratory nitrate reductase 1 beta chain
- Nitrate/nitrite antiporter NarK
- Nitrate reductase molybdenum cofactor assembly chaperone NarJ
- Nitrite reductase small subunit
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0005515 (protein binding), GO:0051540 (metal cluster binding), GO:0016491 (oxidoreductase activity), GO:0009055 (electron transfer activity), GO:0016661 (oxidoreductase activity, acting on other nitrogenous compounds as donors), GO:0051536 (iron-sulfur cluster binding), GO:0051539 (4 iron, 4 sulfur cluster binding), GO:0008940 (nitrate reductase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006091 (generation of precursor metabolites and energy), GO:2001057 (reactive nitrogen species metabolic process), GO:0006082 (organic acid metabolic process), GO:0022900 (electron transport chain), GO:0042126 (nitrate metabolic process), GO:0015980 (energy derivation by oxidation of organic compounds), GO:0043436 (oxoacid metabolic process), GO:0045333 (cellular respiration), GO:0022904 (respiratory electron transport chain), GO:0019645 (anaerobic electron transport chain), GO:0009061 (anaerobic respiration)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0098552 (side of membrane), GO:0016020 (membrane), GO:1902494 (catalytic complex), GO:0071944 (cell periphery), GO:0070469 (respirasome), GO:0098796 (membrane protein complex), GO:0098797 (plasma membrane protein complex), GO:0098803 (respiratory chain complex), GO:0070470 (plasma membrane respirasome), GO:1990204 (oxidoreductase complex), GO:0098562 (cytoplasmic side of membrane), GO:0005886 (plasma membrane), GO:0009898 (cytoplasmic side of plasma membrane)