Protein: Platelet endothelial cell adhesion molecule (PECAM-1) (EndoCAM) (GPIIA') (PECA1) (CD antigen CD31) (P16284)
Organism: Homo sapiens (Human)
Function: Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis (By similarity).
Subcellular location: Cell membrane {ECO:0000269|PubMed:17580308}; Single-pass type I membrane protein {ECO:0000305}. Note=Cell surface expression on neutrophils is down-regulated upon fMLP or CXCL8/IL8-mediated stimulation. {ECO:0000269|PubMed:17580308}.; SUBCELLULAR LOCATION: [Isoform Long]: Cell membrane {ECO:0000269|PubMed:18388311, ECO:0000269|PubMed:19342684}; Single-pass type I membrane protein {ECO:0000305|PubMed:18388311}. Membrane raft {ECO:0000269|PubMed:17139370, ECO:0000269|PubMed:22496122}. Cell junction {ECO:0000269|PubMed:18388311}. Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. {ECO:0000269|PubMed:19342684}.; SUBCELLULAR LOCATION: [Isoform Delta15]: Cell junction {ECO:0000269|PubMed:18388311}. Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
Sequence: MQPRWAQGATMWLGVLLTLLLCSSLEGQENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVSTTSHVKPQHQMLFYKDDVLFYNISSMKSTESYFIPEVRIYDSGTYKCTVIVNNKEKTTAEYQVLVEGVPSPRVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARIISGIHMQTSESTKSELVTVTESFSTPKFHISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQKDKAIVAHNRHGNKAVYSVMAMVEHSGNYTCKVESSRISKVSSIVVNITELFSKPELESSFTHLDQGERLNLSCSIPGAPPANFTIQKEDTIVSQTQDFTKIASKSDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRISYDAQFEVIKGQTIEVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKASKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKKGLIAVVIIGVIIALLIIAAKCYFLRKAKAKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVRNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT
InterPro domains: - IPR036179: Immunoglobulin-like domain superfamily (homologous_superfamily) [40-593]
- IPR007110: Immunoglobulin-like domain (domain) [35-591]
- IPR013783: Immunoglobulin-like fold (homologous_superfamily) [26-596]
- IPR003598: Immunoglobulin subtype 2 (domain) [338-579]
- IPR003599: Immunoglobulin domain subtype (domain) [42-595]
- IPR040878: IL-40-like, Ig domain (domain) [414-485]
- IPR050488: Immunoglobulin Fc region receptor (family) [195-348]
Interaction partners: - Vascular endothelial growth factor receptor 2
- Cadherin-5
- Tyrosine-protein phosphatase non-receptor type 11
- Catenin beta-1
- CD177 antigen
- Cadherin-17
- ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
- Tyrosine-protein phosphatase non-receptor type 6
- E-selectin
- CD44 antigen
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0048870 (cell motility), GO:0090130 (tissue migration), GO:0007155 (cell adhesion), GO:0019222 (regulation of metabolic process), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0040012 (regulation of locomotion), GO:0048522 (positive regulation of cellular process), GO:2000145 (regulation of cell motility), GO:0035556 (intracellular signal transduction), GO:0009611 (response to wounding), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0001568 (blood vessel development), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0048731 (system development), GO:0090132 (epithelium migration), GO:0060255 (regulation of macromolecule metabolic process), GO:0001525 (angiogenesis), GO:0035295 (tube development), GO:0000902 (cell morphogenesis), GO:0009888 (tissue development), GO:0031325 (positive regulation of cellular metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0031589 (cell-substrate adhesion), GO:0016477 (cell migration), GO:0035239 (tube morphogenesis), GO:0010631 (epithelial cell migration), GO:0010562 (positive regulation of phosphorus metabolic process), GO:0051246 (regulation of protein metabolic process), GO:0001944 (vasculature development), GO:0030334 (regulation of cell migration), GO:0001667 (ameboidal-type cell migration), GO:0030855 (epithelial cell differentiation), GO:0007160 (cell-matrix adhesion), GO:0042060 (wound healing), GO:0000904 (cell morphogenesis involved in differentiation), GO:0048514 (blood vessel morphogenesis), GO:0002064 (epithelial cell development), GO:0072359 (circulatory system development), GO:0051247 (positive regulation of protein metabolic process), GO:0007264 (small GTPase mediated signal transduction), GO:0060429 (epithelium development), GO:0051174 (regulation of phosphorus metabolic process), GO:0031399 (regulation of protein modification process), GO:0045937 (positive regulation of phosphate metabolic process), GO:0003382 (epithelial cell morphogenesis), GO:0043542 (endothelial cell migration), GO:0019220 (regulation of phosphate metabolic process), GO:0045446 (endothelial cell differentiation), GO:0007265 (Ras protein signal transduction), GO:0001885 (endothelial cell development), GO:0003158 (endothelium development), GO:0031401 (positive regulation of protein modification process), GO:0042325 (regulation of phosphorylation), GO:0007266 (Rho protein signal transduction), GO:0001932 (regulation of protein phosphorylation), GO:0042327 (positive regulation of phosphorylation), GO:0001934 (positive regulation of protein phosphorylation), GO:0050731 (positive regulation of peptidyl-tyrosine phosphorylation), GO:0042509 (regulation of tyrosine phosphorylation of STAT protein), GO:0050730 (regulation of peptidyl-tyrosine phosphorylation), GO:0042531 (positive regulation of tyrosine phosphorylation of STAT protein)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)