Protein: Lissencephaly-1 homolog (DdLIS1) (Q8I0F4)
Organism: Dictyostelium discoideum (Social amoeba)
Function: Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the coupling of the nucleus and centrosome and dynein/dynactin-mediated interactions of microtubule tips with the cell cortex.
Subcellular location: Cytoplasm, cytoskeleton {ECO:0000255|HAMAP-Rule:MF_03141, ECO:0000269|PubMed:15800059}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000255|HAMAP-Rule:MF_03141, ECO:0000269|PubMed:15800059}. Cytoplasm, cytoskeleton, spindle {ECO:0000255|HAMAP-Rule:MF_03141, ECO:0000269|PubMed:15800059}. Note=Localizes to microtubules and to the centrosome throughout the entire cell cycle. Is part of the centrosomal corona.
Sequence: MVLTSKQKEELNGSILDYFESSQYKSSFEEFKKETGTELDVKKKGLLEKKWTSVIRLQKKVLDLEAKVAQLEEELNSGGGRGGGRGRGKEDALPRPPEKHILTGHRNCINSVKFHPSFSLMVSASEDATIKVWDFESGEFERTLKGHTNAVQDIDFDKTGNLLASCSADLTIKLWDFQTYDCVKTLHGHDHNVSCVRFTPSGDQLISSSRDKTIKVWEAATGYCIKTLVGHEDWVRKITVSEDGSCIASCSNDQTIKTWNIVKGECLATYREHSHVVECLAFSTANIIDIPGSLLSTPEGKSKVKQGPGGNLVGQCGYLATGSRDKTIKIWELATGRCLATYIGHDNWVRAVRFHPCGKFLLSVGDDKTIRVWDIAQGRCIKTINEAHTHFISCLDFCLHNPHIATGGVDDVIKVWKLQ
InterPro domains: - IPR019775: WD40 repeat, conserved site (conserved_site) [121-375]
- IPR001680: WD40 repeat (repeat) [95-419]
- IPR020472: PAC1/LIS1-like, WD-40 repeat (repeat) [121-375]
- IPR015943: WD40/YVTN repeat-like-containing domain superfamily (homologous_superfamily) [99-419]
- IPR050505: WD repeat-containing protein 55/POC1 (family) [103-418]
- IPR017252: Dynein regulator LIS1 (family) [1-418]
- IPR056795: PAC1-like, LisH-like dimerisation domain (domain) [5-38]
- IPR037190: LIS1, N-terminal (homologous_superfamily) [2-72]
- IPR036322: WD40-repeat-containing domain superfamily (homologous_superfamily) [94-416]
- IPR006594: LIS1 homology motif (conserved_site) [7-39]
Interaction partners: - Membrane bound O-acyl transferase family protein
- Microtubule-associated protein RP/EB family member 1
- Dynein heavy chain, cytoplasmic
- Lis-interacting protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0044877 (protein-containing complex binding), GO:0070840 (dynein complex binding)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0007017 (microtubule-based process), GO:0051640 (organelle localization), GO:0007018 (microtubule-based movement), GO:0051299 (centrosome separation), GO:0007059 (chromosome segregation), GO:1903047 (mitotic cell cycle process), GO:0046907 (intracellular transport), GO:0051656 (establishment of organelle localization), GO:0007051 (spindle organization), GO:0006810 (transport), GO:0050000 (chromosome localization), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0000226 (microtubule cytoskeleton organization), GO:0008608 (attachment of spindle microtubules to kinetochore), GO:0000278 (mitotic cell cycle), GO:1902850 (microtubule cytoskeleton organization involved in mitosis), GO:0098813 (nuclear chromosome segregation), GO:0010970 (transport along microtubule), GO:0030705 (cytoskeleton-dependent intracellular transport), GO:0140014 (mitotic nuclear division), GO:0007080 (mitotic metaphase plate congression), GO:0007052 (mitotic spindle organization), GO:0006996 (organelle organization), GO:0000070 (mitotic sister chromatid segregation), GO:0051650 (establishment of vesicle localization), GO:0099111 (microtubule-based transport), GO:0072384 (organelle transport along microtubule), GO:0007010 (cytoskeleton organization), GO:0030473 (nuclear migration along microtubule), GO:0000819 (sister chromatid segregation), GO:0048285 (organelle fission), GO:0051276 (chromosome organization), GO:0000280 (nuclear division)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005815 (microtubule organizing center), GO:0005875 (microtubule associated complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0071944 (cell periphery), GO:0000922 (spindle pole), GO:0098687 (chromosomal region), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0099080 (supramolecular complex), GO:0031616 (spindle pole centrosome), GO:0043229 (intracellular organelle), GO:0005938 (cell cortex), GO:0043228 (non-membrane-bounded organelle), GO:0000776 (kinetochore), GO:0005813 (centrosome), GO:0005869 (dynactin complex), GO:0043227 (membrane-bounded organelle), GO:0000775 (chromosome, centromeric region), GO:0000779 (condensed chromosome, centromeric region), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0030863 (cortical cytoskeleton), GO:0099512 (supramolecular fiber), GO:0099513 (polymeric cytoskeletal fiber), GO:0030864 (cortical actin cytoskeleton), GO:0005634 (nucleus), GO:0005856 (cytoskeleton), GO:0005819 (spindle), GO:0005694 (chromosome), GO:0005874 (microtubule), GO:0015629 (actin cytoskeleton), GO:0015630 (microtubule cytoskeleton), GO:0000793 (condensed chromosome), GO:0005876 (spindle microtubule)