Protein: Elongation factor 1-alpha (EF-1-alpha) (Eukaryotic elongation factor 1A) (eEF1A) (Translation elongation factor 1A) (P02994)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: GTP-binding component of the eukaryotic elongation factor 1 complex (eEF1). In its active GTP-bound form, binds to and delivers aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. In the presence of a correct codon-anticodon match between the aminoacyl-tRNA and the A-site codon of the ribosome-bound mRNA, the ribosome acts as a GTPase activator and the GTP is hydrolyzed. The inactive GDP-bound form leaves the ribosome and must be recycled by its guanine nucleotide exchange factor (GEF) (eEF1B subcomplex) before binding another molecule of aminoacyl-tRNA. Required for nuclear export of aminoacyl-tRNAs. May also be involved in translational quality control by targeting cotranslationally damaged proteins to the proteasome. Also exhibits actin filament-binding and -bundling activities and is involved in cytoskeleton organization. Plays a role as a negative regulator of GCN2 kinase activity by inhibiting GCN2-mediated eIF-2-alpha phosphorylation in amino acid-repleted cells.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:14562095}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:14562095}.
Sequence: MGKEKSHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYQVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGVGEFEAGISKDGQTREHALLAFTLGVRQLIVAVNKMDSVKWDESRFQEIVKETSNFIKKVGYNPKTVPFVPISGWNGDNMIEATTNAPWYKGWEKETKAGVVKGKTLLEAIDAIEQPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPAGVTTEVKSVEMHHEQLEQGVPGDNVGFNVKNVSVKEIRRGNVCGDAKNDPPKGCASFNATVIVLNHPGQISAGYSPVLDCHTAHIACRFDELLEKNDRRSGKKLEDHPKFLKSGDAALVKFVPSKPMCVEAFSEYPPLGRFAVRDMRQTVAVGVIKSVDKTEKAAKVTKAAQKAAKK
InterPro domains: - IPR050100: Translation factor GTPase superfamily members (family) [1-442]
- IPR004161: Translation elongation factor EFTu-like, domain 2 (domain) [258-324]
- IPR004539: Translation elongation factor EF1A, eukaryotic/archaeal (family) [1-444]
- IPR054696: GTP-eEF1A, C-terminal domain (domain) [337-437]
- IPR000795: Translational (tr)-type GTP-binding domain (domain) [5-240]
- IPR009001: Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal (homologous_superfamily) [335-440]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [1-259]
- IPR009000: Translation protein, beta-barrel domain superfamily (homologous_superfamily) [241-332]
- IPR031157: Tr-type G domain, conserved site (conserved_site) [61-76]
Interaction partners: - Elongation factor 1-beta
- Elongation factor 1-gamma 1
- Elongation factor 1-gamma 2
- U3 small nucleolar RNA-associated protein 20
- Ribosome biogenesis protein BMS1
- Elongation factor 3A
- Eukaryotic peptide chain release factor subunit 1
- Actin
- GTP-binding nuclear protein GSP1/CNR1
- Glyceraldehyde-3-phosphate dehydrogenase 3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0045182 (translation regulator activity), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:0090079 (translation regulator activity, nucleic acid binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0008135 (translation factor activity, RNA binding), GO:0003746 (translation elongation factor activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0030029 (actin filament-based process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044085 (cellular component biogenesis), GO:0019538 (protein metabolic process), GO:0016043 (cellular component organization), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0030036 (actin cytoskeleton organization), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0006412 (translation), GO:0006518 (peptide metabolic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0043933 (protein-containing complex organization), GO:0006996 (organelle organization), GO:0022411 (cellular component disassembly), GO:0010467 (gene expression), GO:0097435 (supramolecular fiber organization), GO:0007015 (actin filament organization), GO:0022607 (cellular component assembly), GO:0007010 (cytoskeleton organization), GO:0043043 (peptide biosynthetic process), GO:0022618 (ribonucleoprotein complex assembly), GO:0032984 (protein-containing complex disassembly), GO:0065003 (protein-containing complex assembly), GO:0006415 (translational termination), GO:0002181 (cytoplasmic translation), GO:0071826 (ribonucleoprotein complex subunit organization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)