Protein: Centrosomal protein CCDC61 (Coiled-coil domain-containing protein 61) (VFL3 homolog) (Q08CF3)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Microtubule-binding centrosomal protein required for centriole cohesion, independently of the centrosome-associated protein/CEP250 and rootletin/CROCC linker. In interphase, required for anchoring microtubule at the mother centriole subdistal appendages and for centrosome positioning. During mitosis, may be involved in spindle assembly and chromatin alignment by regulating the organization of spindle microtubules into a symmetrical structure. Plays a non-essential role in ciliogenesis.
Subcellular location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:Q9Y6R9}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite {ECO:0000250|UniProtKB:Q9Y6R9}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q9Y6R9}. Note=Localization at the centriolar satellite is dependent on intact microtubule network. Localizes at the centriole subdistal appendages and proximal ends. Localized to centrosomal/satellite-like structures with the onset of centrosome separation in early G2. {ECO:0000250|UniProtKB:Q9Y6R9}.
Sequence: MEVGTVVQEEMKFRGSEFAVKVEMAERLLIVEISDVVTADQWRGEFGPAYIEDLTRKTGNFKQFPVFCSMLESAVHKSSDSVTLDLLTYSDLELLRNRKAGVVGRPRAQPQSPALSAKRYLILIYTVEFDRIHYPLPLPYLGKPDPAELQKEIRALRSELKTLGLRGDHKVSDQETRKLRTELALVRDEKEALAKALDRLQMVGSGSAPGARGLREAVHSLEEQLLKERAKSQRSAIKKSQEQRLLVEQLEELRASERALRIRVKSLTTELALLRRGRATPVLSDRGGLRGDGVVHRSLSRERSLTRVGIRARSGSRERIEDRGRRSEERVRRADSSGSRNCITRPSPSPTGSRVPRFDPTAYIQDRQRRQKEAELKSQRKIRRDMLASPSLMERGRSRSREPVPQLMRAGSAGRGRSVSVESRRSRCSSEGSVAEFEELAKPLNSRGRKLMSNGPAVSRGRHINKKPMCSTPAQRMRAGDTSMDTGADLSEIDARLQALQDYMRDLDTGH
InterPro domains: - IPR049733: Coiled-coil domain-containing protein 61, N-terminal head domain (domain) [7-140]
Interaction partners: - Inhibitor of growth protein
- Inhibitor of growth protein
- Bromodomain-containing protein 9
- Tissue factor pathway inhibitor
- FACT complex subunit
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0008092 (cytoskeletal protein binding), GO:0015631 (tubulin binding), GO:0008017 (microtubule binding)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0071840 (cellular component organization or biogenesis), GO:0007275 (multicellular organism development), GO:0016043 (cellular component organization), GO:0044085 (cellular component biogenesis), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0072001 (renal system development), GO:0030030 (cell projection organization), GO:0006996 (organelle organization), GO:0001822 (kidney development), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0030031 (cell projection assembly), GO:0120036 (plasma membrane bounded cell projection organization), GO:0044782 (cilium organization), GO:0060271 (cilium assembly), GO:0120031 (plasma membrane bounded cell projection assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005815 (microtubule organizing center), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0036064 (ciliary basal body), GO:0120025 (plasma membrane bounded cell projection), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0005929 (cilium), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0015630 (microtubule cytoskeleton)