Protein: Probable cytosolic iron-sulfur protein assembly protein CIAO1 (WD repeat-containing protein 39) (Q99KN2)
Organism: Mus musculus (Mouse)
Function: Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (By similarity). As a CIA complex component, interacts specifically with CIAO2A or CIAO2B and MMS19 to assist different branches of iron-sulfur protein assembly, depending of its interactors. The complex CIAO1:CIAO2B:MMS19 binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. CIAO1:CIAO2A specifically matures ACO1 and stabilizes IREB2 (By similarity). Seems to specifically modulate the transactivation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (By similarity).
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:O76071}.
Sequence: MKDSLVLQSRVPAHPDSRCWFLAWNPSGTLLASCGGDRKIRIWGTEGDSWICKSVLSEGHQRTVRKVAWSPCGNYLASASFDATTCIWKKNQDDFECVTTLEGHENEVKSVAWAPSGNLLATCSRDKSVWVWEVDEEDEYECVSVLSSHTQDVKHVVWHPSQELLASASYDDTVKLYQEEGDDWVCCATLEGHESTVWSIAFDPSGQRLASCSDDRTVRIWRQYLPGNEQGVACSGSDPSWKCICTLSGFHTRTIYDVAWCQLTGALATACGDDAIRVFEEDPGSDPQQPTFSLTAHLRQAHSQDVNCVAWNPKEPGLLASCSDDGEVAFWEYHQPAGL
InterPro domains: - IPR028608: Probable cytosolic iron-sulfur protein assembly protein, CIAO1/Cia1 (family) [4-335]
- IPR001680: WD40 repeat (repeat) [4-339]
- IPR019775: WD40 repeat, conserved site (conserved_site) [120-134]
- IPR015943: WD40/YVTN repeat-like-containing domain superfamily (homologous_superfamily) [1-339]
- IPR036322: WD40-repeat-containing domain superfamily (homologous_superfamily) [15-331]
Interaction partners: - Cytosolic iron-sulfur assembly component 2B
- MMS19 nucleotide excision repair protein homolog
- Cytosolic iron-sulfur assembly component 2A
- Cytosolic iron-sulfur assembly component 3
- ADP/ATP translocase 2
- General transcription and DNA repair factor IIH helicase subunit XPD
- Cytosolic Fe-S cluster assembly factor NUBP1
- Anamorsin
- Cysteine desulfurase
- Lipoyl synthase, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:1901564 (organonitrogen compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0042127 (regulation of cell population proliferation), GO:0019538 (protein metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0016043 (cellular component organization), GO:0080090 (regulation of primary metabolic process), GO:0043170 (macromolecule metabolic process), GO:0006790 (sulfur compound metabolic process), GO:0008284 (positive regulation of cell population proliferation), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0051604 (protein maturation), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0022607 (cellular component assembly), GO:0051252 (regulation of RNA metabolic process), GO:0016226 (iron-sulfur cluster assembly), GO:0010467 (gene expression), GO:0010468 (regulation of gene expression), GO:2001141 (regulation of RNA biosynthetic process), GO:0097428 (protein maturation by iron-sulfur cluster transfer), GO:0006355 (regulation of DNA-templated transcription), GO:0031163 (metallo-sulfur cluster assembly), GO:0006357 (regulation of transcription by RNA polymerase II), GO:1903506 (regulation of nucleic acid-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)