Protein: Threonine--tRNA ligase editing subunit (Ser-tRNA(Thr) hydrolase) (Threonyl-tRNA synthetase editing subunit) (ThrS-ed) (Q980D1)
Organism: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus)
Function: Freestanding tRNA editing subunit of threonine--tRNA ligase, the catalytic subunit is AC Q97VW8. Deacylates (edits) mischarged L-seryl-tRNA(Thr) in trans, removing L-serine, has no aminoacylation activity. In vitro when both subunits are present, or if the 2 subunits are fused, L-seryl-tRNA(Thr) is no longer produced. Has no activity on correctly acylated L-seryl-tRNA(Ser) or L-threonyl-tRNA(Thr). Editing is probably catalyzed by the 2'-OH of A76 of tRNA(Thr) (By similarity).
Subcellular location: Cytoplasm {ECO:0000305}.
Sequence: MIILFIHASDFSFNVKERAIKEPEEAKLKSIELKNTLVCFTTVEKGDDEEILSKAIDDILDVYSKVKADSVVIYPYAHLSSNLANPDTAIKILESLENLLKDKVKVYRAPFGWYKAFSISCYGHPLSELSRRIRKTEELEKSEELKYCEKFGFPSSSESAFMRRATIGYLRNLFQPLFESENNENVRDGEMSILYQNVESGRILPCINENPRIVVVYGGVRELNFPKEINDSKNRIRVWWVNESKIYVDVGRLIYYFILESVKQQPPTLPDWLNPIQVRLLPVKKDFLDFSIQVAERLRKEGIRVNIDDLDDSLGNKIRRAGTDWIPFVIVIGEREVKTNTLTVKIRARNEQKSMTVEELVKEIKDEVKERQNLPLYYTLYRHKNN
InterPro domains: - IPR047246: Threonine-tRNA ligase, class IIa, anticodon-binding domain (domain) [275-365]
- IPR036621: Anticodon-binding domain superfamily (homologous_superfamily) [268-380]
- IPR004154: Anticodon-binding (domain) [277-366]
- IPR023509: D-aminoacyl-tRNA deacylase-like superfamily (homologous_superfamily) [1-133]
- IPR015011: Threonyl-tRNA synthetase, editing domain, archaea (domain) [1-133]
Interaction partners: - Threonine--tRNA ligase catalytic subunit
- Valine--tRNA ligase
- Probable translation initiation factor IF-2
- Large ribosomal subunit protein uL11
- Small ribosomal subunit protein uS12
- Large ribosomal subunit protein uL15
- Small ribosomal subunit protein uS15
- Small ribosomal subunit protein uS11
- Large ribosomal subunit protein uL3
- Small ribosomal subunit protein uS14
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0010467 (gene expression), GO:0018130 (heterocycle biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0016070 (RNA metabolic process), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006351 (DNA-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0140535 (intracellular protein-containing complex), GO:0030677 (ribonuclease P complex), GO:1990234 (transferase complex), GO:0000428 (DNA-directed RNA polymerase complex), GO:0061695 (transferase complex, transferring phosphorus-containing groups)