Protein: RNA-binding protein 8A (Q5D018)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Required for pre-mRNA splicing as component of the spliceosome (By similarity). Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs (By similarity)
Subcellular location: Nucleus. Nucleus speckle. Cytoplasm. Note=Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA
Sequence: MADVLDLHEAGGEDFPMDEDGDESIHKLKEKAKKRKGRGFGSEEGARSRVREDYDTVEQDGDEPGPQRSVEGWILFVTGVHEEATEEDVHDKFAEFGEIKNLHLNLDRRTGYLKGYALVEYETYKEAQAAMEGLNGQELMGQPISVDWCFVRGPPKSKRRGGRRRSRSPDRRRR
InterPro domains: - IPR033744: RBM8, RNA recognition motif (domain) [67-154]
- IPR008111: RNA-binding motif protein 8 (family) [26-171]
- IPR035979: RNA-binding domain superfamily (homologous_superfamily) [63-173]
- IPR000504: RNA recognition motif domain (domain) [73-151]
- IPR012677: Nucleotide-binding alpha-beta plait domain superfamily (homologous_superfamily) [1-165]
Interaction partners: - Protein mago nashi homolog
- Protein CASC3
- Eukaryotic initiation factor 4A-III
- Partner of Y14 and mago
- Regulator of nonsense transcripts 1
- Nonsense-mediated mRNA decay factor SMG7
- Regulator of nonsense transcripts 3B
- Nuclear cap-binding protein subunit 2
- Serine/threonine-protein kinase SMG1
- Pre-mRNA-splicing factor SLU7
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0003729 (mRNA binding), GO:0003730 (mRNA 3'-UTR binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0051094 (positive regulation of developmental process), GO:0050793 (regulation of developmental process), GO:0050794 (regulation of cellular process), GO:0048522 (positive regulation of cellular process), GO:0045597 (positive regulation of cell differentiation), GO:0045595 (regulation of cell differentiation), GO:1901532 (regulation of hematopoietic progenitor cell differentiation), GO:2000738 (positive regulation of stem cell differentiation), GO:1901534 (positive regulation of hematopoietic progenitor cell differentiation), GO:2000736 (regulation of stem cell differentiation), GO:1902036 (regulation of hematopoietic stem cell differentiation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)