Protein: Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (Q95U38)
Organism: Drosophila melanogaster (Fruit fly)
Function: ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit
Subcellular location: Mitochondrion
Sequence: MASFLARTGGPLIETVRPAAIKKILGLAPIAVQQLRNLNVQEHVSYSLLNEAKIPTPRFAVAKNGKEANDIATKLKTDNLVLKAQVLAGGRGKGTFKNGLKGGVRVVYDPQTAEELSSKMIDQLLVTKQTGAAGRICKKVMVAERKFPRREFYFAVMMERAFNGPVLIASKEGGVDIEEVAASSPDAILYEPIDIGTGLTSEQAEKIVKKVGLGGDGEDTHVQMLLNLYDLFVKKDALLVEINPYAEDAMSGCFFALDAKLRFDDNAEFRQKELFALRDWTQEDPKEVEAAKYNLNYIALDGTIGCMVNGAGLAMATMDIIKLYGGEPANFLDVGGGATAEAVKAAFKIITSDPKVLCILVNIFGGIMRCDVIAEGIISATKDLNLNMPVVVRLQGTKVKEARELIRTSGLKILARDDLDKAADLAVHLAQIVKLAREMKMDVNFEIPDAQKGKGDCKKDQKQPDSKGGKKSEKKDDKKSDCTKKEEKKKEEKKDICEKKGKK
InterPro domains: - IPR034723: Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (family) [35-449]
- IPR013650: ATP-grasp fold, succinyl-CoA synthetase-type (domain) [39-246]
- IPR005809: Succinate--CoA ligase-like, beta subunit (family) [38-431]
- IPR017866: Succinyl-CoA synthetase, beta subunit, conserved site (conserved_site) [302-327]
- IPR013815: ATP-grasp fold, subdomain 1 (homologous_superfamily) [58-148]
- IPR011761: ATP-grasp fold (domain) [46-275]
- IPR016102: Succinyl-CoA synthetase-like (homologous_superfamily) [284-433]
- IPR005811: ATP-citrate synthase/succinyl-CoA ligase, C-terminal domain (domain) [307-426]
Interaction partners: - Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial
- Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial
- Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial
- Dihydrolipoyl dehydrogenase
- Cullin 3, isoform F
- 2-oxoglutarate dehydrogenase, mitochondrial
- AT18465p
- Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
- RH44771p
- Isocitrate dehydrogenase [NADP]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0016877 (ligase activity, forming carbon-sulfur bonds), GO:0016878 (acid-thiol ligase activity), GO:0004774 (succinate-CoA ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0019953 (sexual reproduction), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0022412 (cellular process involved in reproduction in multicellular organism), GO:0019222 (regulation of metabolic process), GO:0009893 (positive regulation of metabolic process), GO:0065009 (regulation of molecular function), GO:0032504 (multicellular organism reproduction), GO:0003006 (developmental process involved in reproduction), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0048609 (multicellular organismal reproductive process), GO:0007276 (gamete generation), GO:0050790 (regulation of catalytic activity), GO:0007281 (germ cell development), GO:0044093 (positive regulation of molecular function), GO:0007349 (cellularization), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0048468 (cell development), GO:0007291 (sperm individualization), GO:0030154 (cell differentiation), GO:0048515 (spermatid differentiation), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0007283 (spermatogenesis), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0051246 (regulation of protein metabolic process), GO:0051336 (regulation of hydrolase activity), GO:0051247 (positive regulation of protein metabolic process), GO:0007286 (spermatid development), GO:0043085 (positive regulation of catalytic activity), GO:0048232 (male gamete generation), GO:0045862 (positive regulation of proteolysis), GO:0030162 (regulation of proteolysis), GO:0051345 (positive regulation of hydrolase activity), GO:0052547 (regulation of peptidase activity), GO:0010952 (positive regulation of peptidase activity), GO:0052548 (regulation of endopeptidase activity), GO:0010950 (positive regulation of endopeptidase activity), GO:2000116 (regulation of cysteine-type endopeptidase activity), GO:2001056 (positive regulation of cysteine-type endopeptidase activity), GO:0043281 (regulation of cysteine-type endopeptidase activity involved in apoptotic process), GO:0043280 (positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0140535 (intracellular protein-containing complex), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:1990234 (transferase complex), GO:0043227 (membrane-bounded organelle), GO:0000151 (ubiquitin ligase complex), GO:0031461 (cullin-RING ubiquitin ligase complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0031463 (Cul3-RING ubiquitin ligase complex)