Protein: Elongation factor G (EF-G) (P13551)
Organism: Thermus thermophilus
Function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Subcellular location: Cytoplasm.
Sequence: MAVKVEYDLKRLRNIGIAAHIDAGKTTTTERILYYTGRIHKIGEVHEGAATMDFMEQERERGITITAAVTTCFWKDHRINIIDTPGHVDFTIEVERSMRVLDGAIVVFDSSQGVEPQSETVWRQAEKYKVPRIAFANKMDKTGADLWLVIRTMQERLGARPVVMQLPIGREDTFSGIIDVLRMKAYTYGNDLGTDIREIPIPEEYLDQAREYHEKLVEVAADFDENIMLKYLEGEEPTEEELVAAIRKGTIDLKITPVFLGSALKNKGVQLLLDAVVDYLPSPLDIPPIKGTTPEGEVVEIHPDPNGPLAALAFKIMADPYVGRLTFIRVYSGTLTSGSYVYNTTKGRKERVARLLRMHANHREEVEELKAGDLGAVVGLKETITGDTLVGEDAPRVILESIEVPEPVIDVAIEPKTKADQEKLSQALARLAEEDPTFRVSTHPETGQTIISGMGELHLEIIVDRLKREFKVDANVGKPQVAYRETITKPVDVEGKFIRQTGGRGQYGHVKIKVEPLPRGSGFEFVNAIVGGVIPKEYIPAVQKGIEEAMQSGPLIGFPVVDIKVTLYDGSYHEVDSSEMAFKIAGSMAIKEAVQKGDPVILEPIMRVEVTTPEEYMGDVIGDLNARRGQILGMEPRGNAQVIRAFVPLAEMFGYATDLRSKTQGRGSFVMFFDHYQEVPKQVQEKLIKGQ
InterPro domains: - IPR031157: Tr-type G domain, conserved site (conserved_site) [53-68]
- IPR004161: Translation elongation factor EFTu-like, domain 2 (domain) [323-389]
- IPR005517: Translation elongation factor EFG/EF2, domain IV (domain) [479-598]
- IPR014721: Small ribosomal subunit protein uS5 domain 2-type fold, subgroup (homologous_superfamily) [485-688]
- IPR041095: Elongation Factor G, domain II (domain) [404-478]
- IPR035647: EF-G domain III/V-like (homologous_superfamily) [402-689]
- IPR000795: Translational (tr)-type GTP-binding domain (domain) [10-284]
- IPR005225: Small GTP-binding domain (domain) [13-181]
- IPR047872: Elongation factor G, domain IV (domain) [483-597]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [4-293]
- IPR009000: Translation protein, beta-barrel domain superfamily (homologous_superfamily) [265-396]
- IPR035649: EFG, domain V (domain) [603-680]
- IPR020568: Ribosomal protein uS5 domain 2-type superfamily (homologous_superfamily) [479-599]
- IPR004540: Translation elongation factor EFG/EF2 (family) [6-691]
- IPR000640: Elongation factor EFG, domain V-like (domain) [600-687]
- IPR009022: Elongation factor G, domain III (domain) [405-480]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0051716 (cellular response to stimulus), GO:0042221 (response to chemical), GO:0070887 (cellular response to chemical stimulus), GO:0010035 (response to inorganic substance), GO:0010038 (response to metal ion), GO:0071241 (cellular response to inorganic substance), GO:0071248 (cellular response to metal ion), GO:0010039 (response to iron ion), GO:0071281 (cellular response to iron ion)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)