Protein: ATPase Get3 (EC 3.6.4.-) (Arsenical pump-driving ATPase) (Arsenite-stimulated ATPase) (Guided entry of tail-anchored proteins factor 3, ATPase) (G3V9T7)
Organism: Rattus norvegicus (Rat)
Function: ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1/WRB and CAMLG/GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-CAMLG receptor, and returning it to the cytosol to initiate a new round of targeting.
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:O43681, ECO:0000255|HAMAP-Rule:MF_03112}. Endoplasmic reticulum {ECO:0000250|UniProtKB:O43681, ECO:0000255|HAMAP-Rule:MF_03112}. Nucleus, nucleolus {ECO:0000250|UniProtKB:O43681, ECO:0000255|HAMAP-Rule:MF_03112}.
Sequence: MAAGVAGWGVEAEEFEDAPDVEPLEPTLSNIIEQRSLKWIFVGGKGGVGKTTCSCSLAVQLSKGRESVLIISTDPAHNISDAFDQKFSKVPTKVKGYDNLFAMEIDPSLGVAELPDEFFEEDNMLSMGKKMMQEAMSAFPGIDEAMSYAEVMRLVKGMNFSVVVFDTAPTGHTLRLLNFPTIVERGLGRLMQIKNQISPFISQMCNMLGLGDMNADQLASKLEETLPVIRSVSEQFKDPEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNIIVNQLVFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTFSALLLEPYKPPSTQ
InterPro domains: - IPR016300: Arsenical pump ATPase, ArsA/GET3 (family) [33-344]
- IPR025723: ArsA/GET3, Anion-transporting ATPase-like domain (domain) [38-339]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [18-348]
- IPR027542: Arsenical pump ATPase, ArsA/GET3, eukaryotic (family) [24-346]
Interaction partners: - Guided entry of tail-anchored proteins factor 1
- Guided entry of tail-anchored proteins factor 4
- Guided entry of tail-anchored proteins factor CAMLG
- Low molecular weight phosphotyrosine protein phosphatase
- Syntaxin-5
- Small glutamine-rich tetratricopeptide repeat-containing protein alpha
- OCA2 melanosomal transmembrane protein
- RAD23 homolog A, nucleotide excision repair protein.
- Peroxisomal ATPase PEX6
- UV excision repair protein RAD23 homolog B
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0051179 (localization), GO:0051234 (establishment of localization), GO:0051641 (cellular localization), GO:0033036 (macromolecule localization), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0046907 (intracellular transport), GO:0045184 (establishment of protein localization), GO:0006810 (transport), GO:0071702 (organic substance transport), GO:0006886 (intracellular protein transport), GO:0071705 (nitrogen compound transport), GO:0008104 (protein localization), GO:0015031 (protein transport), GO:0006605 (protein targeting)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0030054 (cell junction), GO:0045202 (synapse)