Protein: Probable adenylyltransferase/sulfurtransferase MoeZ [Includes: Sulfur carrier protein CysO adenylyltransferase (EC 2.7.7.-); Sulfur carrier protein CysO sulfurtransferase (EC 2.8.1.-)] (P9WMN7)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps: first, ATP-dependent activation of CysO as acyl-adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO-thiocarboxylate (CysO-COSH) (Probable). The sulfur source is unknown.
Subcellular location: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
Sequence: MSTSLPPLVEPASALSREEVARYSRHLIIPDLGVDGQKRLKNARVLVIGAGGLGAPTLLYLAAAGVGTIGIVDFDVVDESNLQRQVIHGVADVGRSKAQSARDSIVAINPLIRVRLHELRLAPSNAVDLFKQYDLILDGTDNFATRYLVNDAAVLAGKPYVWGSIYRFEGQASVFWEDAPDGLGVNYRDLYPEPPPPGMVPSCAEGGVLGIICASVASVMGTEAIKLITGIGETLLGRLLVYDALEMSYRTITIRKDPSTPKITELVDYEQFCGVVADDAAQAAKGSTITPRELRDWLDSGRKLALIDVRDPVEWDIVHIDGAQLIPKSLINSGEGLAKLPQDRTAVLYCKTGVRSAEALAAVKKAGFSDAVHLQGGIVAWAKQMQPDMVMY
InterPro domains: - IPR001763: Rhodanese-like domain (domain) [290-390]
- IPR036873: Rhodanese-like domain superfamily (homologous_superfamily) [275-389]
- IPR045886: ThiF/MoeB/HesA family (family) [17-205]
- IPR000594: THIF-type NAD/FAD binding fold (domain) [23-259]
- IPR035985: Ubiquitin-activating enzyme-like (homologous_superfamily) [10-359]
Interaction partners: - Sulfur carrier protein CysO
- Molybdopterin synthase catalytic subunit 1
- Molybdopterin synthase sulfur carrier subunit
- Molybdopterin synthase sulfur carrier subunit
- IscS-like cysteine desulfurase
- O-phosphoserine sulfhydrylase
- Probable adenylyltransferase/sulfurtransferase MoeZ
- Molybdopterin synthase catalytic subunit 2
- Thiazole synthase
- Molybdopterin synthase catalytic subunit 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0016874 (ligase activity), GO:0016740 (transferase activity), GO:0008641 (ubiquitin-like modifier activating enzyme activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0016782 (transferase activity, transferring sulphur-containing groups), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016877 (ligase activity, forming carbon-sulfur bonds), GO:0042802 (identical protein binding), GO:0016775 (phosphotransferase activity, nitrogenous group as acceptor), GO:0016783 (sulfurtransferase activity), GO:0016301 (kinase activity), GO:0061629 (RNA polymerase II-specific DNA-binding transcription factor binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0019538 (protein metabolic process), GO:0043170 (macromolecule metabolic process), GO:0006790 (sulfur compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0036211 (protein modification process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0043412 (macromolecule modification), GO:0044272 (sulfur compound biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)