Protein: Actin-related protein 2 (Actin-like protein ARP2) (Actin-like protein 2) (P32381)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament (By similarity).
Subcellular location: Cytoplasm, cytoskeleton, actin patch {ECO:0000269|PubMed:11248049, ECO:0000269|PubMed:9210376}.
Sequence: MDPHNPIVLDQGTGFVKIGRAGENFPDYTFPSIVGRPILRAEERASVATPLKDIMIGDEASEVRSYLQISYPMENGIIKNWTDMELLWDYAFFEQMKLPSTSNGKILLTEPPMNPLKNREKMCEVMFEKYDFGGVYVAIQAVLALYAQGLSSGVVVDSGDGVTHIVPVYESVVLSHLTRRLDVAGRDVTRHLIDLLSRRGYAFNRTADFETVRQIKEKLCYVSYDLDLDTKLARETTALVESYELPDGRTIKVGQERFEAPECLFQPGLVDVEQPGVGELLFNTVQSADVDIRSSLYKAIVLSGGSSMYPGLPSRLEKELKQLWFSRVLHNDPSRLDKFKVRIEDPPRRKHMVFIGGAVLASIMADKDHMWLSKQEWQESGPSAMTKFGPR
InterPro domains: - IPR043129: ATPase, nucleotide binding domain (homologous_superfamily) [3-386]
- IPR004000: Actin family (family) [2-391]
- IPR020902: Actin/actin-like conserved site (conserved_site) [107-119]
Interaction partners: - Actin-related protein 2/3 complex subunit 5
- Actin-related protein 3
- Actin-related protein 2/3 complex subunit 4
- Actin-related protein 2/3 complex subunit 2
- Actin-related protein 2/3 complex subunit 1
- Proline-rich protein LAS17
- Actin-related protein 2/3 complex subunit 3
- Actin
- Cofilin
- Protein AIM7
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0044877 (protein-containing complex binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:0005515 (protein binding), GO:0051015 (actin filament binding), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0032553 (ribonucleotide binding), GO:0008092 (cytoskeletal protein binding), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0032555 (purine ribonucleotide binding), GO:0003779 (actin binding), GO:0017076 (purine nucleotide binding), GO:0005524 (ATP binding), GO:0032559 (adenyl ribonucleotide binding), GO:0030554 (adenyl nucleotide binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0030029 (actin filament-based process), GO:0016043 (cellular component organization), GO:0030036 (actin cytoskeleton organization), GO:0030866 (cortical actin cytoskeleton organization), GO:0006996 (organelle organization), GO:0097435 (supramolecular fiber organization), GO:0044396 (actin cortical patch organization), GO:0007015 (actin filament organization), GO:0007010 (cytoskeleton organization), GO:0045010 (actin nucleation), GO:0034314 (Arp2/3 complex-mediated actin nucleation), GO:0030865 (cortical cytoskeleton organization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0061645 (endocytic patch), GO:0030427 (site of polarized growth), GO:0005933 (cellular bud), GO:0071944 (cell periphery), GO:0060187 (cell pole), GO:0140535 (intracellular protein-containing complex), GO:0031975 (envelope), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0099080 (supramolecular complex), GO:0031967 (organelle envelope), GO:0031090 (organelle membrane), GO:0051286 (cell tip), GO:0120025 (plasma membrane bounded cell projection), GO:0043332 (mating projection tip), GO:0043229 (intracellular organelle), GO:0005938 (cell cortex), GO:0030479 (actin cortical patch), GO:0043228 (non-membrane-bounded organelle), GO:0099081 (supramolecular polymer), GO:0005635 (nuclear envelope), GO:0005934 (cellular bud tip), GO:0005935 (cellular bud neck), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005937 (mating projection), GO:0030863 (cortical cytoskeleton), GO:0099512 (supramolecular fiber), GO:0099513 (polymeric cytoskeletal fiber), GO:0030864 (cortical actin cytoskeleton), GO:0005634 (nucleus), GO:0005856 (cytoskeleton), GO:0015629 (actin cytoskeleton), GO:0005884 (actin filament)