Protein: Axin-like protein pry-1 (Protein polyray) (O62090)
Organism: Caenorhabditis elegans
Function: Works in parallel with axl-1 in negatively regulating bar-1 signaling in vulval precursor cells and Q neuroblasts. Inhibits Wnt signaling, which affects tissue specific expression of Hox genes, egl-5, lin-39 and mab-5. This in turn affects QR (postembryonic neuroblast) cell migration, vulval cell fate specification, and the development of sensory structures by the seam cell lineage. Has a role in alae V cell patterning, ray formation in the male tail and axon guidance. Does not affect B cell polarity.
Subcellular location: Cell membrane {ECO:0000269|PubMed:12023307, ECO:0000269|PubMed:16077004}. Nucleus {ECO:0000269|PubMed:12023307, ECO:0000269|PubMed:16077004}. Cytoplasm, cell cortex {ECO:0000269|PubMed:12023307, ECO:0000269|PubMed:16077004}. Note=Subcellular location was measured using a GFP reporter gene. Location of the pry-1:GFP fusion protein ranges from plasma membrane and cytoplasmic dots to diffuse cytoplasmic and nuclear staining. This difference may be due to variations in expression levels of the fusion protein in different transgenic lines. {ECO:0000269|PubMed:12023307, ECO:0000269|PubMed:16077004}.
Sequence: METHLGWARSLEAVLSDRSALDAFQEWLIEYSSPQYLDLFFAIRAYERMALEGKPEKSQLSKSIYSKFLSSRTGNCEAIPKHFRAPIGEKLRHGTELEDRVFSHCSNFVQEFLRRQHEEFVGSEEFIEAFNKMSSTTADQLPGGSAHHSSHQNTMRRSSGTTSRKSAAQIATQLTAEALLKSKHDRHSKLGETKLEKMYPPTRQPYVCNATTSHNDSAVSSTFSGDTPEAHRMHSNRLRHIRDEQARENHGTMTLPRVEKASVDGQQWDHSSESGRRNFAMEITRKLLRHIDKVKLNDEMEKRIDDIEECRYTTIDMVNGTEPNDDLGKIDEDEELDDYLKMKMTDDSQKGSTNRSPKGPAGEPNKSGEGSKNTTLSPTNRAPAQLHNTIRVPRRKDYPRDTSASLKSHRHHQIDTNRMMSQSMCAPSYSSASSSYSRDSFAPAPTTRVNFAPGSSKSSQFYDSSGIGSMAPSAFSATSSLDYKDRRQHRKAPTPKKHSKIGKNLSNLITISYLGTDKIPVVTHVPNDGPMTLAEFKRHFALPNGAHQLFFKTECEDGSAPFQLLLIKDEHHLLPVFEGRIAAELR
InterPro domains: - IPR044926: RGS, subdomain 2 (homologous_superfamily) [1-135]
- IPR043581: Axin-like (family) [3-439]
- IPR029071: Ubiquitin-like domain superfamily (homologous_superfamily) [509-585]
- IPR016137: RGS domain (domain) [10-130]
- IPR038207: DIX domain superfamily (homologous_superfamily) [505-586]
- IPR036305: RGS domain superfamily (homologous_superfamily) [2-135]
- IPR001158: DIX domain (domain) [505-586]
Interaction partners: - Beta-catenin/armadillo-related protein 1
- Armadillo repeat-containing protein wrm-1
- Glycogen synthase kinase-3
- Adenomatous polyposis coli protein-related protein 1
- DiSHevelled related
- DiSHevelled related
- Casein kinase I isoform alpha
- Beta-catenin-like protein hmp-2
- Putative serine/threonine-protein kinase gskl-1
- Protein kinase domain-containing protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0019899 (enzyme binding), GO:0005102 (signaling receptor binding), GO:0019900 (kinase binding), GO:0030971 (receptor tyrosine kinase binding), GO:0019901 (protein kinase binding), GO:1990782 (protein tyrosine kinase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009628 (response to abiotic stimulus), GO:0048856 (anatomical structure development), GO:0051641 (cellular localization), GO:0007275 (multicellular organism development), GO:0023057 (negative regulation of signaling), GO:0009653 (anatomical structure morphogenesis), GO:0008340 (determination of adult lifespan), GO:0048870 (cell motility), GO:0007163 (establishment or maintenance of cell polarity), GO:0022402 (cell cycle process), GO:0007389 (pattern specification process), GO:0007049 (cell cycle), GO:0051234 (establishment of localization), GO:0051301 (cell division), GO:0071840 (cellular component organization or biogenesis), GO:0007267 (cell-cell signaling), GO:0050793 (regulation of developmental process), GO:0008219 (cell death), GO:0040017 (positive regulation of locomotion), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0006950 (response to stress), GO:0048523 (negative regulation of cellular process), GO:0048585 (negative regulation of response to stimulus), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0051640 (organelle localization), GO:0040012 (regulation of locomotion), GO:0048522 (positive regulation of cellular process), GO:0032506 (cytokinetic process), GO:0009968 (negative regulation of signal transduction), GO:0010648 (negative regulation of cell communication), GO:0090068 (positive regulation of cell cycle process), GO:0009798 (axis specification), GO:0012501 (programmed cell death), GO:1903047 (mitotic cell cycle process), GO:0030010 (establishment of cell polarity), GO:2000145 (regulation of cell motility), GO:0198738 (cell-cell signaling by wnt), GO:0006979 (response to oxidative stress), GO:0050920 (regulation of chemotaxis), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0009966 (regulation of signal transduction), GO:1905114 (cell surface receptor signaling pathway involved in cell-cell signaling), GO:0018991 (oviposition), GO:0051649 (establishment of localization in cell), GO:0048608 (reproductive structure development), GO:0045137 (development of primary sexual characteristics), GO:0035262 (gonad morphogenesis), GO:0000226 (microtubule cytoskeleton organization), GO:0051255 (spindle midzone assembly), GO:0032886 (regulation of microtubule-based process), GO:0044089 (positive regulation of cellular component biogenesis), GO:2000147 (positive regulation of cell motility), GO:0044087 (regulation of cellular component biogenesis), GO:0045787 (positive regulation of cell cycle), GO:0051726 (regulation of cell cycle), GO:0048580 (regulation of post-embryonic development), GO:0009887 (animal organ morphogenesis), GO:0048598 (embryonic morphogenesis), GO:0104004 (cellular response to environmental stimulus), GO:0046907 (intracellular transport), GO:0003002 (regionalization), GO:0010646 (regulation of cell communication), GO:0051656 (establishment of organelle localization), GO:0007186 (G protein-coupled receptor signaling pathway), GO:0071214 (cellular response to abiotic stimulus), GO:0006810 (transport), GO:0097190 (apoptotic signaling pathway), GO:0009790 (embryo development), GO:0016043 (cellular component organization), GO:0007548 (sex differentiation), GO:0051128 (regulation of cellular component organization), GO:0010564 (regulation of cell cycle process), GO:0000278 (mitotic cell cycle), GO:0016477 (cell migration), GO:1902850 (microtubule cytoskeleton organization involved in mitosis), GO:0061458 (reproductive system development), GO:0007346 (regulation of mitotic cell cycle), GO:0007088 (regulation of mitotic nuclear division), GO:0043254 (regulation of protein-containing complex assembly), GO:0016477 (cell migration), GO:0008406 (gonad development), GO:0032465 (regulation of cytokinesis), GO:0009792 (embryo development ending in birth or egg hatching), GO:0006796 (phosphate-containing compound metabolic process), GO:0051294 (establishment of spindle orientation), GO:0030334 (regulation of cell migration), GO:0070986 (left/right axis specification), GO:0016331 (morphogenesis of embryonic epithelium), GO:0051294 (establishment of spindle orientation), GO:0030334 (regulation of cell migration), GO:0070986 (left/right axis specification), GO:1902903 (regulation of supramolecular fiber organization), GO:0010639 (negative regulation of organelle organization), GO:0061064 (negative regulation of nematode larval development), GO:0060972 (left/right pattern formation), GO:0051293 (establishment of spindle localization), GO:1901987 (regulation of cell cycle phase transition), GO:0033043 (regulation of organelle organization), GO:0006996 (organelle organization), GO:0010638 (positive regulation of organelle organization), GO:0009952 (anterior/posterior pattern specification), GO:0000281 (mitotic cytokinesis), GO:0009792 (embryo development ending in birth or egg hatching), GO:0061024 (membrane organization), GO:0051493 (regulation of cytoskeleton organization), GO:0040001 (establishment of mitotic spindle localization), GO:0051494 (negative regulation of cytoskeleton organization), GO:0032271 (regulation of protein polymerization), GO:0009794 (regulation of mitotic cell cycle, embryonic), GO:0030832 (regulation of actin filament length), GO:0048285 (organelle fission), GO:1901880 (negative regulation of protein depolymerization), GO:0007010 (cytoskeleton organization), GO:1902412 (regulation of mitotic cytokinesis), GO:1903436 (regulation of mitotic cytokinetic process), GO:1903438 (positive regulation of mitotic cytokinetic process), GO:1901990 (regulation of mitotic cell cycle phase transition), GO:0000132 (establishment of mitotic spindle orientation), GO:0099024 (plasma membrane invagination), GO:0000280 (nuclear division)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)