Protein: Aconitate hydratase, mitochondrial (Aconitase) (EC 4.2.1.3) (Citrate hydro-lyase) (P20004)
Organism: Bos taurus (Bovine)
Function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Subcellular location: Mitochondrion {ECO:0000255}.
Sequence: MAPYSLLVSRLQKALGARQYHVASVLCQRAKVAMSHFEPNEYIRYDLLEKNINIVRKRLNRPLTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPDSGCHYDQLIEINLSELKPHINGPFTPDLAHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLQCKSQFTITPGSEQIRATIERDGYAQILRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRAEFDPGQDTYQHPPKDSSGQQVDVSPTSQRLQLLEPFDKWDGRDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINVENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPADYNKIHPVDKLTIKGLKDFAPGKPLTCIIKHPNGTQETILLNHTFNETQIEWFRAGSALNRMKELQK
InterPro domains: - IPR015931: Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3 (homologous_superfamily) [29-517]
- IPR015928: Aconitase/3-isopropylmalate dehydratase, swivel (homologous_superfamily) [561-780]
- IPR018136: Aconitase family, 4Fe-4S cluster binding site (binding_site) [377-453]
- IPR006248: Aconitase, mitochondrial-like (family) [45-775]
- IPR001030: Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain (domain) [67-503]
- IPR000573: Aconitase A/isopropylmalate dehydratase small subunit, swivel domain (domain) [583-711]
- IPR050926: Aconitase/IPM Isomerase (family) [11-779]
- IPR036008: Aconitase, iron-sulfur domain (homologous_superfamily) [33-550]
- IPR015932: Aconitase, domain 2 (homologous_superfamily) [229-345]
Interaction partners: - Citrate synthase, mitochondrial
- Isocitrate dehydrogenase subunit alpha, mitochondrial
- Isocitrate dehydrogenase, mitochondrial
- Isocitrate dehydrogenase cytoplasmic
- Isocitrate dehydrogenase subunit beta, mitochondrial
- ATP-citrate synthase
- Succinate dehydrogenase flavoprotein subunit, mitochondrial
- 2-oxoglutarate dehydrogenase complex component E1
- Citrate synthase
- Succinate dehydrogenase iron-sulfur subunit, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016829 (lyase activity), GO:0016835 (carbon-oxygen lyase activity), GO:0016836 (hydro-lyase activity), GO:0003994 (aconitate hydratase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0044238 (primary metabolic process), GO:0006091 (generation of precursor metabolites and energy), GO:0006099 (tricarboxylic acid cycle), GO:0015980 (energy derivation by oxidation of organic compounds), GO:0045333 (cellular respiration), GO:0009060 (aerobic respiration)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0005759 (mitochondrial matrix), GO:0070013 (intracellular organelle lumen), GO:0043231 (intracellular membrane-bounded organelle)