Protein: Mitotic spindle assembly checkpoint protein MAD2A (Mitotic arrest deficient 2-like protein 1) (MAD2-like protein 1) (Q556Y9)
Organism: Dictyostelium discoideum (Social amoeba)
Function: Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex until all chromosomes are aligned at the metaphase plate (By similarity).
Subcellular location: Nucleus {ECO:0000250}. Chromosome, centromere, kinetochore {ECO:0000250}. Cytoplasm {ECO:0000250}.
Sequence: MQAAVASKTNISLKGSTEIVTEFFSYSINTILFQRGLYPPESFTRVAKYGLPILVTNDQSLKDYLDNVLKQLSEWLLSGDVQKLVLVITDIVTKEVLERWVFDVTTDIPKEGEAPRQKPEKEIMNEIQAIIRQITASVTFLPLLPNACTFDLLVYTSKDLAVPQKWEESDPKFITNSQQVKLRSFTTTIHKVESMVAYKISND
InterPro domains: - IPR003511: HORMA domain (domain) [14-196]
- IPR045091: Mad2-like (family) [8-201]
- IPR036570: HORMA domain superfamily (homologous_superfamily) [1-203]
Interaction partners: - Probable inactive serine/threonine-protein kinase bub1
- Anaphase-promoting complex subunit 4
- Anaphase-promoting complex subunit cdc20
- Mitotic checkpoint protein bub3
- Probable serine/threonine-protein kinase mps1
- Anaphase-promoting complex subunit 6
- Anaphase-promoting complex subunit 1
- Anaphase-promoting complex subunit 8
- Anaphase-promoting complex subunit cdh1
- Anaphase-promoting complex subunit 10
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009628 (response to abiotic stimulus), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0048523 (negative regulation of cellular process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:1903047 (mitotic cell cycle process), GO:0051129 (negative regulation of cellular component organization), GO:0035556 (intracellular signal transduction), GO:0009314 (response to radiation), GO:0010948 (negative regulation of cell cycle process), GO:0051726 (regulation of cell cycle), GO:0033554 (cellular response to stress), GO:0045786 (negative regulation of cell cycle), GO:0051128 (regulation of cellular component organization), GO:0010564 (regulation of cell cycle process), GO:0000278 (mitotic cell cycle), GO:0007346 (regulation of mitotic cell cycle), GO:0007088 (regulation of mitotic nuclear division), GO:0000075 (cell cycle checkpoint signaling), GO:0006974 (cellular response to DNA damage stimulus), GO:0042770 (signal transduction in response to DNA damage), GO:0007093 (mitotic cell cycle checkpoint signaling), GO:0010639 (negative regulation of organelle organization), GO:0051983 (regulation of chromosome segregation), GO:1901987 (regulation of cell cycle phase transition), GO:0045930 (negative regulation of mitotic cell cycle), GO:1905819 (negative regulation of chromosome separation), GO:0033043 (regulation of organelle organization), GO:0051985 (negative regulation of chromosome segregation), GO:1901988 (negative regulation of cell cycle phase transition), GO:0031570 (DNA integrity checkpoint signaling), GO:0051783 (regulation of nuclear division), GO:0051784 (negative regulation of nuclear division), GO:0010965 (regulation of mitotic sister chromatid separation), GO:0033045 (regulation of sister chromatid segregation), GO:1901991 (negative regulation of mitotic cell cycle phase transition), GO:2000816 (negative regulation of mitotic sister chromatid separation), GO:0033046 (negative regulation of sister chromatid segregation), GO:1902099 (regulation of metaphase/anaphase transition of cell cycle), GO:0033048 (negative regulation of mitotic sister chromatid segregation), GO:0031577 (spindle checkpoint signaling), GO:0045841 (negative regulation of mitotic metaphase/anaphase transition), GO:0033047 (regulation of mitotic sister chromatid segregation), GO:1901990 (regulation of mitotic cell cycle phase transition), GO:1902100 (negative regulation of metaphase/anaphase transition of cell cycle), GO:2001251 (negative regulation of chromosome organization), GO:0033044 (regulation of chromosome organization), GO:0071174 (mitotic spindle checkpoint signaling), GO:0007094 (mitotic spindle assembly checkpoint signaling), GO:0071173 (spindle assembly checkpoint signaling), GO:0030071 (regulation of mitotic metaphase/anaphase transition)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0000922 (spindle pole), GO:0031975 (envelope), GO:0140513 (nuclear protein-containing complex), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005654 (nucleoplasm), GO:0031967 (organelle envelope), GO:0044615 (nuclear pore nuclear basket), GO:0005643 (nuclear pore), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043233 (organelle lumen), GO:0005635 (nuclear envelope), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0005634 (nucleus), GO:0005856 (cytoskeleton), GO:0031981 (nuclear lumen), GO:0005819 (spindle), GO:0072686 (mitotic spindle), GO:0015630 (microtubule cytoskeleton)