Protein: Eukaryotic initiation factor 4A-I (P60842)
Organism: Homo sapiens (Human)
Function: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. As a result, promotes cell proliferation and growth
Subcellular location: Cytoplasm, perinuclear region. Cell membrane. Cytoplasm, Stress granule. Note=Colocalizes with PKP1 in stress granules following arsenate or hydrogen peroxide treatment
Sequence: MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI
InterPro domains: - IPR044728: ATP-dependent RNA helicase eIF4A, DEAD-box helicase domain (domain) [34-235]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [57-222]
- IPR001650: Helicase, C-terminal domain-like (domain) [245-406]
- IPR014014: RNA helicase, DEAD-box type, Q motif (domain) [32-60]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [20-406]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [51-249]
- IPR000629: ATP-dependent RNA helicase DEAD-box, conserved site (conserved_site) [180-188]
Interaction partners: - Eukaryotic translation initiation factor 4E
- Polyadenylate-binding protein 1
- Programmed cell death protein 4
- Eukaryotic translation initiation factor 4H
- Eukaryotic translation initiation factor 4 gamma 3
- Eukaryotic translation initiation factor 4 gamma 1
- Eukaryotic translation initiation factor 4B
- Eukaryotic translation initiation factor 4 gamma 2
- Eukaryotic translation initiation factor 3 subunit B
- Eukaryotic translation initiation factor 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0065009 (regulation of molecular function), GO:0048523 (negative regulation of cellular process), GO:0009892 (negative regulation of metabolic process), GO:0050790 (regulation of catalytic activity), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0044092 (negative regulation of molecular function), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0043086 (negative regulation of catalytic activity), GO:0034248 (regulation of amide metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0006446 (regulation of translational initiation), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0051338 (regulation of transferase activity), GO:0051253 (negative regulation of RNA metabolic process), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0010468 (regulation of gene expression), GO:0051348 (negative regulation of transferase activity), GO:2000112 (regulation of cellular macromolecule biosynthetic process), GO:0006417 (regulation of translation), GO:2001141 (regulation of RNA biosynthetic process), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0010608 (post-transcriptional regulation of gene expression), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0048471 (perinuclear region of cytoplasm), GO:0016281 (eukaryotic translation initiation factor 4F complex), GO:0005737 (cytoplasm)