Protein: Glutathione S-transferase DHAR1, mitochondrial (EC 2.5.1.18) (Chloride intracellular channel homolog 1) (CLIC homolog 1) (Glutathione-dependent dehydroascorbate reductase 1) (AtDHAR1) (GSH-dependent dehydroascorbate reductase 1) (mtDHAR) (EC 1.8.5.1) (Q9FWR4)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Displays a dual function. As a soluble protein, exhibits glutathione-dependent thiol transferase and dehydroascorbate (DHA) reductase activities. Key component of the ascorbate recycling system. Involved in the redox homeostasis, especially in scavenging of ROS under oxidative stresses, subsequently to biotic or abiotic inducers. As a peripheral membrane protein, could also function as voltage-gated ion channel.
Subcellular location: Mitochondrion {ECO:0000269|PubMed:12954611}. Cytoplasm, cytosol {ECO:0000305}. Peroxisome {ECO:0000269|PubMed:19329564}. Membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}. Note=Exists both as soluble protein and as membrane protein.
Sequence: MALEICVKAAVGAPDHLGDCPFSQRALLTLEEKSLTYKIHLINLSDKPQWFLDISPQGKVPVLKIDDKWVTDSDVIVGILEEKYPDPPLKTPAEFASVGSNIFGTFGTFLKSKDSNDGSEHALLVELEALENHLKSHDGPFIAGERVSAVDLSLAPKLYHLQVALGHFKSWSVPESFPHVHNYMKTLFSLDSFEKTKTEEKYVISGWAPKVNP
InterPro domains: - IPR036249: Thioredoxin-like superfamily (homologous_superfamily) [18-87]
- IPR036282: Glutathione S-transferase, C-terminal domain superfamily (homologous_superfamily) [83-211]
- IPR044627: Dehydroascorbate reductases DHAR1/2/3/4 (family) [1-210]
- IPR010987: Glutathione S-transferase, C-terminal-like (domain) [66-213]
- IPR040079: Glutathione transferase family (family) [19-187]
- IPR004045: Glutathione S-transferase, N-terminal (domain) [10-88]
Interaction partners: - Monodehydroascorbate reductase 3
- Glutathione reductase, chloroplastic
- L-galactono-1,4-lactone dehydrogenase, mitochondrial
- L-ascorbate peroxidase 3
- Putative glutathione peroxidase 7, chloroplastic
- Phospholipid hydroperoxide glutathione peroxidase 1, chloroplastic
- Probable phospholipid hydroperoxide glutathione peroxidase 6, mitochondrial
- Probable glutathione peroxidase 2
- Probable glutathione peroxidase 3, mitochondrial
- Probable glutathione peroxidase 4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016209 (antioxidant activity), GO:0043167 (ion binding), GO:0016491 (oxidoreductase activity), GO:0043168 (anion binding), GO:0016667 (oxidoreductase activity, acting on a sulfur group of donors), GO:0015036 (disulfide oxidoreductase activity), GO:0016672 (oxidoreductase activity, acting on a sulfur group of donors, quinone or similar compound as acceptor), GO:0043295 (glutathione binding), GO:0015038 (glutathione disulfide oxidoreductase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0023056 (positive regulation of signaling), GO:0007275 (multicellular organism development), GO:0044237 (cellular metabolic process), GO:0048584 (positive regulation of response to stimulus), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0023051 (regulation of signaling), GO:0071704 (organic substance metabolic process), GO:0003006 (developmental process involved in reproduction), GO:0044238 (primary metabolic process), GO:0009791 (post-embryonic development), GO:0048522 (positive regulation of cellular process), GO:0048316 (seed development), GO:0009967 (positive regulation of signal transduction), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0010646 (regulation of cell communication), GO:0046483 (heterocycle metabolic process), GO:0006082 (organic acid metabolic process), GO:0048731 (system development), GO:0019538 (protein metabolic process), GO:0009966 (regulation of signal transduction), GO:0010647 (positive regulation of cell communication), GO:0048608 (reproductive structure development), GO:0005996 (monosaccharide metabolic process), GO:0005975 (carbohydrate metabolic process), GO:0006766 (vitamin metabolic process), GO:0043170 (macromolecule metabolic process), GO:0036211 (protein modification process), GO:1901334 (lactone metabolic process), GO:0061458 (reproductive system development), GO:0043412 (macromolecule modification), GO:0006767 (water-soluble vitamin metabolic process), GO:0043436 (oxoacid metabolic process), GO:0019852 (L-ascorbic acid metabolic process), GO:0010154 (fruit development), GO:2000031 (regulation of salicylic acid mediated signaling pathway), GO:0019752 (carboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0071944 (cell periphery), GO:0005737 (cytoplasm), GO:0016020 (membrane), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0043231 (intracellular membrane-bounded organelle)