Protein: E3 ubiquitin-protein ligase Godzilla (EC 2.3.2.27) (Q9VI20)
Organism: Drosophila melanogaster (Fruit fly)
Function: Endosomal E3 ubiquitin-protein ligase that regulates the recycling endosome pathway by mediating ubiquitination of Synaptobrevin (Syb). Also acts as a regulator of transcytosis in wing imaginal disks by catalyzing ubiquitination of Syb: ubiquitination of Syb promotes transcytosis of wingless (wg) to the basolateral surface.
Subcellular location: Endosome membrane {ECO:0000269|PubMed:23353890}; Single-pass type I membrane protein {ECO:0000255}.
Sequence: MSKRSCQILTLLGLCLVCHEATLVGGHVLVYRKATSQLIEEFNDLPAQFGPNLPSNGLKVYVVPARRPYYGCDSLDRPPHLKYPPSAKFVALVARGECVFERKIRVAQNASYSAVIVYNNEGDDLEQMSAENITGIRIPSVFVGHTTGKALATYFTTEVVLIINDELPFNINTQLILPFSILIGMCFIIMVIYMIYKCIREQRRLRRHRLPKSMLKKLPVLRYTKNNANNKYDTCVICLEDFIEDDKLRVLPCSHPYHTHCIDPWLTENRRVCPICKRKVFTKGEARASRSRQPSLDNVTDTDDDTTPLLQQQQSNGRQVGQVSSASSAGGAAGSSSSVAAAAVAGTTRHGTFRRGHAGRNPFEESQSSDDENALLASTVRPATSSGAHERINPFDRAPNLPAHLAEQLTESRRSVWSRINFASFFRRQPAVISVAAPPYLERVESGTSAMGLPVTGTIAVASPASNNILNPNLSGSFKDEDDMPPHRSIYEPIAISTPAADSATVDDSAFLQTPTQGGIGVAALPHSASDRQFLI
InterPro domains: - IPR051653: E3 ubiquitin-protein ligase and sorting receptor (family) [95-286]
- IPR046450: PA domain superfamily (homologous_superfamily) [89-152]
- IPR044744: ZNRF4 /RNF13/RNF167, PA domain (domain) [14-171]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [210-285]
- IPR003137: PA domain (domain) [65-151]
- IPR001841: Zinc finger, RING-type (domain) [233-277]
Interaction partners: - Kinesin-like protein Klp98A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0019787 (ubiquitin-like protein transferase activity), GO:0004842 (ubiquitin-protein transferase activity), GO:0061659 (ubiquitin-like protein ligase activity), GO:0061630 (ubiquitin protein ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0051179 (localization), GO:0051234 (establishment of localization), GO:0051641 (cellular localization), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0033036 (macromolecule localization), GO:0006807 (nitrogen compound metabolic process), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0046907 (intracellular transport), GO:1901575 (organic substance catabolic process), GO:0043170 (macromolecule metabolic process), GO:0045184 (establishment of protein localization), GO:0006810 (transport), GO:0009057 (macromolecule catabolic process), GO:0071702 (organic substance transport), GO:0006886 (intracellular protein transport), GO:0071705 (nitrogen compound transport), GO:0008104 (protein localization), GO:1901565 (organonitrogen compound catabolic process), GO:0015031 (protein transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043227 (membrane-bounded organelle), GO:0031982 (vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0097708 (intracellular vesicle)