Protein: Dual-action ribosomal maturation protein DarP (P0A8X0)
Organism: Escherichia coli (strain K12)
Function: Member of a network of 50S ribosomal subunit biogenesis factors (ObgE, RluD, RsfS and DarP) which assembles along the 30S-50S interface, preventing incorrect 23S rRNA structures from forming. Promotes peptidyl transferase center (PTC) maturation. Binds pre-50S ribosomal subunits near the 23S rRNA L1 stalk, where it may mediate correct positioning of the L1 stalk. Plays a dual role in the late stages of assembly of the 50S ribosomal subunit; promotes peptidyl transferase center (PTC) maturation by modulating the docking of 23S rRNA helix H68, and maintains the stability of helix H89 with the help of uL16 (rplP), facilitating the final maturation of the PTC (Probable). Overexpression slows growth at 37 degrees Celsius
Subcellular location: Cytoplasm. Note=Associates with late stage pre-50S ribosomal subunits
Sequence: MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGAEIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALDKLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKEGNKPPKSARQIFQYLRELAENEG
InterPro domains: - IPR006839: Dual-action ribosomal maturation protein DarP (family) [1-182]
- IPR023153: Dual-action ribosomal maturation protein DarP superfamily (homologous_superfamily) [37-183]
Interaction partners: - Ribosomal large subunit pseudouridine synthase D
- Ribosomal silencing factor RsfS
- Ribosomal silencing factor RsfS
- Ribosomal silencing factor RsfS
- GTPase ObgE/CgtA
- Large ribosomal subunit protein bL33
- RNA-binding protein YhbY
- Large ribosomal subunit protein uL24
- Large ribosomal subunit protein bL32
- Large ribosomal subunit protein bL17
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0003729 (mRNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0048523 (negative regulation of cellular process), GO:0009892 (negative regulation of metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0034248 (regulation of amide metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0051248 (negative regulation of protein metabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression), GO:0034249 (negative regulation of amide metabolic process), GO:0017148 (negative regulation of translation), GO:2000112 (regulation of cellular macromolecule biosynthetic process), GO:0006417 (regulation of translation), GO:2000113 (negative regulation of cellular macromolecule biosynthetic process), GO:0010608 (post-transcriptional regulation of gene expression)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)