Protein: Protein DA1 (Protein SUPPRESSOR OF LARGE SEED AND ORGAN PHENOTYPES OF DA1-1 1) (P0C7Q8)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Ubiquitin receptor that limits final seed and organ size by restricting the period of cell proliferation. May act maternally to control seed mass. Acts synergistically with DA2 to regulate seed size. Functions synergistically with DA2 to restrict cell proliferation in the maternal integuments of ovules and developing seeds. Functions antagonistically in a common pathway with UBP15 to regulate seed size. Associates physically with UBP15 and modulates the stability of UBP15, which promote cell proliferation in the integuments of ovules and developing seeds. Functions as peptidase and cleaves the N-terminal sequence of E3 ubiquitin-protein ligases BB and DA2 in a ubiquitin-dependent manner. Cleaves the deubiquitinating enzyme UBP15, which promotes cell proliferation, and the transcription factors TCP15 and TCP22, which promote cell proliferation and repress endoreduplication. Involved in the promotion of leaf senescence, in addition to its function in restricting plant growth. Acts redundantly with DAR1 and DAR2 to regulate endoreduplication during leaf development. Together with DAR1 and DAR2, modulates the protein stability of the transcription factors TCP14 and TCP15, which repress endoreduplication by directly regulating the expression of cell-cycle genes.
Sequence: MGWFNKIFKGSNQRLRVGNNKHNHNVYYDNYPTASHDDEPSAADTDADNDEPHHTQEPSTSEDNTSNDQENEDIDRAIALSLLEENQEQTSISGKYSMPVDEDEQLARALQESMVVGNSPRHKSGSTYDNGNAYGAGDLYGNGHMYGGGNVYANGDIYYPRPITFQMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGCSQPISEYEFSTSGNYPFHKACYRERYHPKCDVCSHFIPTNHAGLIEYRAHPFWVQKYCPSHEHDATPRCCSCERMEPRNTRYVELNDGRKLCLECLDSAVMDTMQCQPLYLQIQNFYEGLNMKVEQEVPLLLVERQALNEAREGEKNGHYHMPETRGLCLSEEQTVSTVRKRSKHGTGKWAGNITEPYKLTRQCEVTAILILFGLPRLLTGSILAHEMMHAWMRLKGFRTLSQDVEEGICQVMAHKWLDAELAAGSTNSNAASSSSSSQGLKKGPRSQYERKLGEFFKHQIESDASPVYGDGFRAGRLAVHKYGLRKTLEHIQMTGRFPV
InterPro domains: - IPR045218: Protein DA1-like (family) [3-531]
- IPR022087: Protein DA1-like domain (domain) [321-527]
- IPR003903: Ubiquitin interacting motif (conserved_site) [69-120]
- IPR001781: Zinc finger, LIM-type (domain) [170-230]
Interaction partners: - HSP20-like chaperones superfamily protein
- AT3g15090/K15M2_24
- DEAD-box ATP-dependent RNA helicase 28
- Protein BOBBER 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0032182 (ubiquitin-like protein binding), GO:0008233 (peptidase activity), GO:0043130 (ubiquitin binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0051726 (regulation of cell cycle), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0043170 (macromolecule metabolic process), GO:0010564 (regulation of cell cycle process), GO:0036211 (protein modification process), GO:0007346 (regulation of mitotic cell cycle), GO:0043412 (macromolecule modification), GO:1901987 (regulation of cell cycle phase transition), GO:0045930 (negative regulation of mitotic cell cycle), GO:0070647 (protein modification by small protein conjugation or removal), GO:1901990 (regulation of mitotic cell cycle phase transition), GO:0032446 (protein modification by small protein conjugation), GO:0016567 (protein ubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)