Protein: Phosphoribosylformylglycinamidine synthase subunit PurL (FGAM synthase) (EC 6.3.5.3) (Formylglycinamide ribonucleotide amidotransferase subunit II) (FGAR amidotransferase II) (FGAR-AT II) (Glutamine amidotransferase PurL) (Phosphoribosylformylglycinamidine synthase subunit II) (P9WHL7)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.
Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00420}.
Sequence: MSPLARTPRKTSVLDTVEHAATTPDQPQPYGELGLKDDEYRRIRQILGRRPTDTELAMYSVMWSEHCSYKSSKVHLRYFGETTSDEMRAAMLAGIGENAGVVDIGDGWAVTFKVESHNHPSYVEPYQGAATGVGGIVRDIMAMGARPVAVMDQLRFGAADAPDTRRVLDGVVRGIGGYGNSLGLPNIGGETVFDPCYAGNPLVNALCVGVLRQEDLHLAFASGAGNKIILFGARTGLDGIGGVSVLASDTFDAEGSRKKLPSVQVGDPFMEKVLIECCLELYAGGLVIGIQDLGGAGLSCATSELASAGDGGMTIQLDSVPLRAKEMTPAEVLCSESQERMCAVVSPKNVDAFLAVCRKWEVLATVIGEVTDGDRLQITWHGETVVDVPPRTVAHEGPVYQRPVARPDTQDALNADRSAKLSRPVTGDELRATLLALLGSPHLCSRAFITEQYDRYVRGNTVLAEHADGGMLRIDESTGRGIAVSTDASGRYTLLDPYAGAQLALAEAYRNVAVTGATPVAVTNCLNFGSPEDPGVMWQFTQAVRGLADGCADLGIPVTGGNVSFYNQTGSAAILPTPVVGVLGVIDDVRRRIPTGLGAEPGETLMLLGDTRDEFDGSVWAQVTADHLGGLPPVVDLAREKLLAAVLSSASRDGLVSAAHDLSEGGLAQAIVESALAGETGCRIVLPEGADPFVLLFSESAGRVLVAVPRTEESRFRGMCEARGLPAVRIGVVDQGSDAVEVQGLFAVSLAELRATSEAVLPRYFG
InterPro domains: - IPR041609: Phosphoribosylformylglycinamidine synthase, linker domain (domain) [28-70]
- IPR010074: Phosphoribosylformylglycinamidine subunit PurL (family) [1-766]
- IPR036921: PurM-like, N-terminal domain superfamily (homologous_superfamily) [30-593]
- IPR010918: PurM-like, C-terminal domain (domain) [224-737]
- IPR016188: PurM-like, N-terminal domain (domain) [96-586]
- IPR036676: PurM-like, C-terminal domain superfamily (homologous_superfamily) [46-765]
Interaction partners: - Phosphoribosylformylglycinamidine synthase subunit PurQ
- Phosphoribosylformylglycinamidine synthase subunit PurS
- Phosphoribosylformylglycinamidine cyclo-ligase
- Phosphoribosylglycinamide formyltransferase
- Amidophosphoribosyltransferase
- Phosphoribosylglycinamide formyltransferase 2
- Phosphoribosylamine--glycine ligase
- N5-carboxyaminoimidazole ribonucleotide synthase
- N5-carboxyaminoimidazole ribonucleotide mutase
- Bifunctional purine biosynthesis protein PurH
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0016874 (ligase activity), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0032553 (ribonucleotide binding), GO:0016879 (ligase activity, forming carbon-nitrogen bonds), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0016884 (carbon-nitrogen ligase activity, with glutamine as amido-N-donor), GO:0032555 (purine ribonucleotide binding), GO:0017076 (purine nucleotide binding), GO:0005524 (ATP binding), GO:0032559 (adenyl ribonucleotide binding), GO:0030554 (adenyl nucleotide binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006793 (phosphorus metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0090407 (organophosphate biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0009117 (nucleotide metabolic process), GO:0006163 (purine nucleotide metabolic process), GO:0072522 (purine-containing compound biosynthetic process), GO:1901293 (nucleoside phosphate biosynthetic process), GO:0009165 (nucleotide biosynthetic process), GO:0006164 (purine nucleotide biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0071944 (cell periphery), GO:0005737 (cytoplasm), GO:0016020 (membrane), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0005886 (plasma membrane), GO:0009274 (peptidoglycan-based cell wall)