Protein: Bud site selection protein 7 (Q08754)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Member of the CHS5-ARF1P-binding proteins (CHAPS) which mediates export of specific cargo proteins, including chitin synthase CHS3. May be involved in positioning the proximal bud pole signal.
Subcellular location: Golgi apparatus, trans-Golgi network membrane {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:16498409, ECO:0000269|PubMed:17000877}; Peripheral membrane protein {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:16498409, ECO:0000269|PubMed:17000877}. Note=Trans-Golgi network location requires interaction with CHS5 and with myristoylated GTP-bound ARF1 for the recruitment to the membranes.
Sequence: MITQNSIPEVKEDFIGYALHERRIRLPQFQDLGPADLVTLTKYLPTSSNTNAINSTSRNGAAIIQSPAAVVADDSAASMATNGDASDTAVTTNYTNASIYSSSRNANDGAPMVAELHPLDKLKDEVGTFFYSMGVDTSGPTSIAIFLKEISEVISEKPQVWFGRKKTFNVARISFSTWNAFRRCDINVVVHIPGSIQNFIVDCNGESQNIEMCADYDLIWAETFVSGVVRSIMLMKENAEEGELQNLVETLILNPFTAGQIDDVPEMFIDLFPIVYHKGPLLGAPYYITNVTNTNNYLVETLVEIVKLTRNVSRAEIMLKNLATDNPEAIIILIKIFLVCDQELDAIKLTYDMLSQDKIINNTNNRMDYKSELLCLQAQFLIDKRQDYSLAQNIAQEAVNCSPSEFRPWYLLSKVYVKLNDIENALLILNSCPMSPLKEKYVLKRVAPLPSNNSLHLPLPIDVVLDEVTSLNPQDVQNEHRSADPMLVNLAASNLKSTFQLAYRLLTEIVQITGWENLLKYRSNIFVMEEEYQKSSSSLPKDVNKQEEQPLRAKRLCERWLDNLFMLLYEDLKMYTLWQTEQLYMDAQNNNHNKLTFEWELFGLCARRLGHFPEAAKAFQNGLSQRFSSRCARKLLEYCINERQRVKNFINSPNSHDMVPEIVSSRIRELDNSIIDLCVKICCWNHRWYTEFSISLLDCLSVVIQDMSLTKVSNEISSRYPETVLNLVQENLLNFFTTCTIGCYDA
InterPro domains: - IPR011990: Tetratricopeptide-like helical domain superfamily (homologous_superfamily) [1-742]
- IPR015374: Chs5p-Arf1p binding (family) [3-535]
Interaction partners: - Chitin biosynthesis protein CHS5
- Chitin biosynthesis protein CHS6
- Protein BCH1
- Protein BCH2
- ADP-ribosylation factor 1
- Chitin synthase 3
- Bud site selection protein 9
- [PSI+] induction protein 2
- Putative uncharacterized protein YOR300W
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0019899 (enzyme binding), GO:0051020 (GTPase binding), GO:0031267 (small GTPase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0009058 (biosynthetic process), GO:0071554 (cell wall organization or biogenesis), GO:0006807 (nitrogen compound metabolic process), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0071852 (fungal-type cell wall organization or biogenesis), GO:0044085 (cellular component biogenesis), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0042546 (cell wall biogenesis), GO:0006810 (transport), GO:0051668 (localization within membrane), GO:0006037 (cell wall chitin metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0006038 (cell wall chitin biosynthetic process), GO:0098876 (vesicle-mediated transport to the plasma membrane), GO:0006040 (amino sugar metabolic process), GO:0016192 (vesicle-mediated transport), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0009272 (fungal-type cell wall biogenesis), GO:0006022 (aminoglycan metabolic process), GO:0006030 (chitin metabolic process), GO:0048193 (Golgi vesicle transport), GO:1901071 (glucosamine-containing compound metabolic process), GO:0006893 (Golgi to plasma membrane transport), GO:0046349 (amino sugar biosynthetic process), GO:0006023 (aminoglycan biosynthetic process), GO:1901073 (glucosamine-containing compound biosynthetic process), GO:0006031 (chitin biosynthetic process), GO:0006892 (post-Golgi vesicle-mediated transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0030133 (transport vesicle), GO:0005798 (Golgi-associated vesicle), GO:0030135 (coated vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0030140 (trans-Golgi network transport vesicle), GO:0031982 (vesicle), GO:0030136 (clathrin-coated vesicle), GO:0097708 (intracellular vesicle)