Protein: Serine/threonine-protein kinase RIO2 (EC 2.7.11.1) (RIO kinase 2) (Q9BVS4)
Organism: Homo sapiens (Human)
Function: Serine/threonine-protein kinase involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in export of the 40S pre-ribosome particles (pre-40S) from the nucleus to the cytoplasm. Its kinase activity is required for the release of NOB1, PNO1 and LTV1 from the late pre-40S and the processing of 18S-E pre-rRNA to the mature 18S rRNA. Regulates the timing of the metaphase-anaphase transition during mitotic progression, and its phosphorylation, most likely by PLK1, regulates this function.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:16037817, ECO:0000269|PubMed:19564402}. Note=Exported out of the nucleus via its NES in a XPO1-dependent manner. {ECO:0000269|PubMed:19564402}.
Sequence: MGKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLIAWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFALKLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYNRHAVVMELINGYPLCQIHHVEDPASVYDEAMELIVKLANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYESELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEKNRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRDEENVGAMNQYRTRTLSITSSGSAVSCSTIPPELVKQKVKRQLTKQQKSAVRRRLQKGEANIFTKQRRENMQNIKSSLEAASFWGE
InterPro domains: - IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [97-272]
- IPR015285: RIO2 kinase winged helix domain, N-terminal (domain) [9-91]
- IPR036390: Winged helix DNA-binding domain superfamily (homologous_superfamily) [8-91]
- IPR000687: RIO kinase (domain) [66-289]
- IPR030484: Serine/threonine-protein kinase Rio2 (domain) [94-276]
- IPR018934: RIO-type domain (domain) [108-279]
- IPR018935: RIO kinase, conserved site (conserved_site) [224-235]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [2-93]
Interaction partners: - Pre-rRNA-processing protein TSR1 homolog
- Protein LTV1 homolog
- RNA-binding protein NOB1
- Small ribosomal subunit protein uS5
- Bystin
- Small ribosomal subunit protein uS19
- Small ribosomal subunit protein uS7
- Small ribosomal subunit protein uS3
- RNA-binding protein PNO1
- KRR1 small subunit processome component homolog
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0044085 (cellular component biogenesis), GO:1901564 (organonitrogen compound metabolic process), GO:0035556 (intracellular signal transduction), GO:0019538 (protein metabolic process), GO:0016043 (cellular component organization), GO:0043170 (macromolecule metabolic process), GO:0006793 (phosphorus metabolic process), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0030030 (cell projection organization), GO:0006796 (phosphate-containing compound metabolic process), GO:0006996 (organelle organization), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0030031 (cell projection assembly), GO:0006468 (protein phosphorylation), GO:0120036 (plasma membrane bounded cell projection organization), GO:0044782 (cilium organization), GO:0016310 (phosphorylation), GO:0120031 (plasma membrane bounded cell projection assembly), GO:0060271 (cilium assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus)