Protein: DNA (cytosine-5)-methyltransferase 3A (Dnmt3a) (EC 2.1.1.37) (Cysteine methyltransferase DNMT3A) (EC 2.1.1.-) (Q4W5Z4)
Organism: Gallus gallus (Chicken)
Function: Required for genome-wide de novo methylation and is essential for development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity. Also has weak auto-methylation activity on some Cys residue in absence of DNA.
Subcellular location: Nucleus {ECO:0000250|UniProtKB:Q9Y6K1}. Chromosome {ECO:0000250|UniProtKB:Q9Y6K1}. Cytoplasm {ECO:0000250|UniProtKB:Q9Y6K1}. Note=Accumulates in the major satellite repeats at pericentric heterochromatin. {ECO:0000250|UniProtKB:O88508}.
Sequence: MVESSDTPKDTAAVPKCPPPCPEASPAEPLPNGDLEADGAQWKGTEEGGASPKSGRPEEDETESLADGETGRALENGRCTPKEGLDAPADEGELAPSDPQKKRGRRKLLEATEKSKEEKEENNFDSLKMEGSRGRLRGGLGWESSLRQRPMQRHTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIAVMNVVEETPRAEPQKEEEASPPASQQPTDPASPNVATTPEPVVADAVDKNTSKSADDEPEYEDGRGLGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLLPLSSFSSAFHQATYNKQPMYRKAIYEVLQVASSRAGKIFPACPENDETDTSKVVEIQNKQMIEWALGGFQPSGPKGLEPPEEERNPYKEVYTEMWVEPEAAAYAPPPPAKKPRKSTTEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFIGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAIKEDPWNCYMCGHKGVYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRNVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHEARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV
InterPro domains: - IPR040552: DNMT3, cysteine rich ADD domain, GATA1-like zinc finger (domain) [440-495]
- IPR000313: PWWP domain (domain) [255-339]
- IPR044108: DNA (cytosine-5)-methyltransferase 3A, ADD domain (domain) [452-579]
- IPR025766: ADD domain (domain) [447-579]
- IPR054724: DNA (cytosine-5)-methyltransferase, N-terminal (domain) [2-249]
- IPR049554: DNMT3, ADD domain, PHD zinc finger (domain) [502-551]
- IPR050390: DNA Cytosine-5 Methyltransferase (family) [97-706]
- IPR018117: DNA methylase, C-5 cytosine-specific, active site (active_site) [667-679]
- IPR029063: S-adenosyl-L-methionine-dependent methyltransferase superfamily (homologous_superfamily) [587-877]
- IPR001525: C-5 cytosine methyltransferase (family) [599-877]
Interaction partners: - DNA
- Uncharacterized protein.
- Histone H4
- Adenosylhomocysteinase
- Methionine adenosyltransferase 2 subunit beta
- S-adenosylmethionine synthase
- AdoHcyase_NAD domain-containing protein
- Adenosylhomocysteinase like 2
- S-adenosylmethionine decarboxylase proenzyme
- tRNA
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:1901363 (heterocyclic compound binding), GO:0016740 (transferase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:0003676 (nucleic acid binding), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0140097 (catalytic activity, acting on DNA), GO:0009008 (DNA-methyltransferase activity), GO:0008168 (methyltransferase activity), GO:0003677 (DNA binding), GO:0003886 (DNA (cytosine-5-)-methyltransferase activity), GO:0008757 (S-adenosylmethionine-dependent methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0032259 (methylation), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0043414 (macromolecule methylation), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0006259 (DNA metabolic process), GO:0006306 (DNA methylation), GO:0006304 (DNA modification), GO:0032776 (DNA methylation on cytosine), GO:0006305 (DNA alkylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043229 (intracellular organelle), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)