Protein: DASH complex subunit DAM1 (Q5APC8)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Component of the DASH complex that connects microtubules with kinetochores and couples microtubule depolymerisation to chromosome movement; it is involved in retrieving kinetochores to the spindle poles before their re-orientation on the spindle in early mitosis and allows microtubule depolymerization to pull chromosomes apart and resist detachment during anaphase. Kinetochores, consisting of a centromere-associated inner segment and a microtubule- contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and microtubules. Kinetochores also serve as an input point for the spindle assembly checkpoint, which delays anaphase until all chromosomes have bioriented on the mitotic spindle
Subcellular location: Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Note=Localization to the kinetochore is independent of microtubules
Sequence: MSSSKPVTPRNDRRQNSGRRHSHRSSGIQLPSMSPKVHHYPIDAENLPMDSPEIVEKFASLAESMETLDLHMHDLCHIHDHISNQFNESFASFLYGLSMTMWCVDFPGCPSREQWEALISKRERKERIEVLKKRLADAQKLNDRLKLRLSDEAKQKPQQLTSQQRPTHGPTRQIKQQPRSVNFNTHSSDSISTNSYTSGDFNTNRRVSRIPQPSRSNLPSSRFQAPSKSGPNLNQPPRYMRGLFDGNNTLNTSNYSRIKKPIHNRSVNNLQNRPPFR
InterPro domains: - IPR013962: DASH complex subunit Dam1 (family) [3-277]
Interaction partners: - DASH complex subunit DAD1
- DASH complex subunit DAD2
- DASH complex subunit SPC34
- DASH complex subunit DAD3
- DASH complex subunit SPC19
- Spindle pole body component
- DASH complex subunit ASK1
- DASH complex subunit DUO1
- DASH complex subunit DAD4
- Mitochondrial 54S ribosomal protein YmL11
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0008092 (cytoskeletal protein binding), GO:0015631 (tubulin binding), GO:0008017 (microtubule binding), GO:0051010 (microtubule plus-end binding)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0022414 (reproductive process), GO:0009987 (cellular process), GO:0000003 (reproduction), GO:0051641 (cellular localization), GO:0051321 (meiotic cell cycle), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:1903046 (meiotic cell cycle process), GO:0051640 (organelle localization), GO:0061982 (meiosis I cell cycle process), GO:0007059 (chromosome segregation), GO:0140013 (meiotic nuclear division), GO:1903047 (mitotic cell cycle process), GO:0051656 (establishment of organelle localization), GO:0050000 (chromosome localization), GO:0051316 (attachment of spindle microtubules to kinetochore involved in meiotic chromosome segregation), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0045132 (meiotic chromosome segregation), GO:0008608 (attachment of spindle microtubules to kinetochore), GO:0000278 (mitotic cell cycle), GO:0045143 (homologous chromosome segregation), GO:0051315 (attachment of mitotic spindle microtubules to kinetochore), GO:0098813 (nuclear chromosome segregation), GO:0140014 (mitotic nuclear division), GO:0007080 (mitotic metaphase plate congression), GO:0031134 (sister chromatid biorientation), GO:0006996 (organelle organization), GO:0007127 (meiosis I), GO:0000070 (mitotic sister chromatid segregation), GO:0051455 (monopolar spindle attachment to meiosis I kinetochore), GO:0051303 (establishment of chromosome localization), GO:0051310 (metaphase plate congression), GO:0000819 (sister chromatid segregation), GO:1990758 (mitotic sister chromatid biorientation), GO:0048285 (organelle fission), GO:0051276 (chromosome organization), GO:0000280 (nuclear division)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098687 (chromosomal region), GO:0000940 (outer kinetochore), GO:0140513 (nuclear protein-containing complex), GO:0099080 (supramolecular complex), GO:0042729 (DASH complex), GO:0000776 (kinetochore), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000775 (chromosome, centromeric region), GO:0043228 (non-membrane-bounded organelle), GO:0000779 (condensed chromosome, centromeric region), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome), GO:0005634 (nucleus), GO:0000793 (condensed chromosome)