Protein: 25S rRNA (cytosine-C(5))-methyltransferase NOP2A (EC 2.1.1.-) (Nucleolar protein 2A) (Protein OLIGOCELLULA 2) (tRNA methyltransferase 4c) (AtTRM4c) (Q9FG73)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Involved in ribosomal large subunit assembly, required for normal progression of rRNA processing. S-adenosyl-L-methionine-dependent methyltransferase that probably methylates the C(5) position of cytosine 2268 (m5C2268) in nuclear 25S rRNA. May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation (By similarity). Seems involved in the regulation of cell proliferation in leaves.
Subcellular location: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768}. Nucleus, nucleolus {ECO:0000269|PubMed:29375609}.
Sequence: MPALTRNKKKAATKSITPPTKQLTKSKTPPMKPQTSMLKKGAKSQNKPPLKKQKKEVVEEEPLEDYEVTDDSDEDDEVSDGSDEDDISPAVESEEIDESDDGENGSNQLFSDDEEENDEETLGDDFLEGSGDEDEEGSLDADSDADSDDDDIVAKSDAIDRDLAMQKKDAAAELEDFIKQDDVHDEEPEHDAFRLPTEEELEEEARGPPDLPLLKTRIEEIVRALKNFKAFRPKDTTRKACVEQLKADLGSYYGYNSFLIGTLVEMFPPGELMELIEAFEKQRPTSIRTNTLKTRRRDLADVLLNRGVNLDPLSKWSKVGLVIYDSQVPIGATPEYLAGYYMLQGASSFLPVMALAPRENERIVDVAAAPGGKTTYIAALMKNTGLIYANEMKVPRLKSLTANLHRMGVTNTIVCNYDGRELPKVLGQNTVDRVLLDAPCSGTGIISKDESVKITKTMDEIKKFAHLQKQLLLAAIDMVDANSKTGGYIVYSTCSIMVTENEAVIDYALKKRDVKLVTCGLDFGRKGFTRFREHRFQPSLDKTRRFYPHVHNMDGFFVAKLKKMSNVKQSSEEGDDDAVETVEQAEVSSDDDDEAEAIEETEKPSVPVRQPKERKEKKNKEKLAKSKEDKRGKKDKKSKSENVEEPSKPRKQKKKRREWKNEIAQAREEKRIAMREKAKEEK
InterPro domains: - IPR023273: RNA (C5-cytosine) methyltransferase, NOP2 (family) [238-307]
- IPR011023: Nop2p (domain) [287-563]
- IPR023267: RNA (C5-cytosine) methyltransferase (family) [168-567]
- IPR029063: S-adenosyl-L-methionine-dependent methyltransferase superfamily (homologous_superfamily) [249-571]
- IPR018314: RsmB/NOL1/NOP2-like, conserved site (conserved_site) [431-442]
- IPR001678: SAM-dependent methyltransferase RsmB-F/NOP2-type domain (domain) [275-564]
- IPR049560: SAM-dependent methyltransferase RsmB-F/NOP2-type, catalytic core (domain) [352-562]
Interaction partners: - Pescadillo homolog
- Ribosome biogenesis protein BOP1 homolog
- Probable rRNA-processing protein EBP2 homolog
- AT5g04600/T32M21_200
- Ribosome assembly factor mrt4
- 60S ribosome subunit biogenesis protein NIP7 homolog
- Nucleolar complex-associated protein 3
- Ribosome production factor 2 homolog
- Probable ribosome biogenesis protein RLP24
- Ribosome biogenesis protein WDR12 homolog
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0016740 (transferase activity), GO:0140098 (catalytic activity, acting on RNA), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0140102 (catalytic activity, acting on a rRNA), GO:0008173 (RNA methyltransferase activity), GO:0008168 (methyltransferase activity), GO:0008169 (C-methyltransferase activity), GO:0008649 (rRNA methyltransferase activity), GO:0008757 (S-adenosylmethionine-dependent methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0022414 (reproductive process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0003006 (developmental process involved in reproduction), GO:0009791 (post-embryonic development), GO:0048316 (seed development), GO:0009790 (embryo development), GO:0048608 (reproductive structure development), GO:0009793 (embryo development ending in seed dormancy), GO:0048731 (system development), GO:0061458 (reproductive system development), GO:0010154 (fruit development)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0043229 (intracellular organelle), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus)