wanglab/bioreason-pro-rl-reasoning-data huggingface dataset:
Protein: maleylacetoacetate isomerase (EC 5.2.1.2) (X1WBU0)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Not known
Sequence: MAAQTKPVLYGYYRSSCSWRVRIAFALKGIEYEQKPINLIKDGGQQLTDQFKAINPMQQVPAVSIDGITLSQSLAIIQYIEETRPEPRLLPADPMQRAHVRIICDIIASGIQPLQNLYVLQKIGEDKVQWAQHFINRGFQALEPVLKETAGKYCVGDEISMADICLVPQVYNADRFKVDMTQYPTIRRLNQTLVEIEAFKASHPSRQPDTPDDLRL
InterPro domains: - IPR040079: Glutathione transferase family (family) [9-192]
- IPR036249: Thioredoxin-like superfamily (homologous_superfamily) [6-111]
- IPR034330: Glutathione S-transferases, class Zeta , C-terminal (domain) [94-207]
- IPR036282: Glutathione S-transferase, C-terminal domain superfamily (homologous_superfamily) [88-211]
- IPR034333: Glutathione S-transferases, class Zeta , N-terminal (domain) [7-81]
- IPR004046: Glutathione S-transferase, C-terminal (domain) [127-195]
- IPR010987: Glutathione S-transferase, C-terminal-like (domain) [93-212]
- IPR005955: Glutathione S-transferases, class Zeta (family) [9-210]
- IPR004045: Glutathione S-transferase, N-terminal (domain) [5-88]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016765 (transferase activity, transferring alkyl or aryl (other than methyl) groups), GO:0004364 (glutathione transferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0051716 (cellular response to stimulus), GO:0042221 (response to chemical), GO:0009410 (response to xenobiotic stimulus), GO:0070887 (cellular response to chemical stimulus), GO:0071466 (cellular response to xenobiotic stimulus)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)
Protein: cyclic-guanylate-specific phosphodiesterase (EC 3.1.4.52) (Q9I025)
Organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Function: Not known
Subcellular location: Cell membrane {ECO:0000256|ARBA:ARBA00004651}; Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}.
Sequence: MQIGRPRRDSLFHFGIVVAALAAVPLLMAPLVYQQARMRAEGEAAVTSVAILRQLDTLLGVVSDSLDKSQEAVGKPCREVLGRLTRMTMLSPYFRSLVLVERESVYCSSLRGELHDLPLQVAFKALGSLPAGQRITPSNATPQVPERASVIMSRGDENGSGVLAIIDGQYLLDIQQAASYDERFQVQMVLRQSQRQLPGGAPATGDGVAVAASARFPVEVRVAVAPALASAYRADAWRHYAPFILLAALLAGYLAHLFCRRRLSLVGDMYRAMRAREFHMVYQPIIHLDTGECRGVEALVRWQRPDRSQVRPDIFIPLAEDNGMIGDLTRHIFGLVAADLAQLGLGAGDHLGVNVSGSHLASHGFVDDVRRLLGAIGSEGPQLVLEVTEREALPHDAQLQHNIQQLRELGVQWALDDFGTGQSSLSHLQKLHADFLKIDRSFVSSVGSGSVNAVVLETIIALAQRLDLAMTAEGIETREQEQYLCGHSIQWGQGYLFSPPLKAAELWAWRNSRRGAEREARVAAS
InterPro domains: - IPR024744: Putative cyclic diguanylate phosphodiesterase, CSS motif-containing domain (domain) [43-235]
- IPR001633: EAL domain (domain) [261-514]
- IPR050706: Cyclic di-GMP phosphodiesterase-like (family) [220-510]
- IPR035919: EAL domain superfamily (homologous_superfamily) [261-522]
Interaction partners: - Diguanylate cyclase
- Diguanylate cyclase TpbB
- GGDEF domain-containing protein
- Diguanylate cyclase
- diguanylate cyclase
- diguanylate cyclase
- Sensor domain-containing diguanylate cyclase
- diguanylate cyclase
- GGDEF domain-containing protein
- diguanylate cyclase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0042578 (phosphoric ester hydrolase activity), GO:0008081 (phosphoric diester hydrolase activity), GO:0071111 (cyclic-guanylate-specific phosphodiesterase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0051716 (cellular response to stimulus), GO:0009628 (response to abiotic stimulus), GO:0042221 (response to chemical), GO:0070887 (cellular response to chemical stimulus), GO:0070482 (response to oxygen levels), GO:0071453 (cellular response to oxygen levels)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)
Protein: Large ribosomal subunit protein uL15 (60S ribosomal protein L27a) (L29) (O15883)
Organism: Trypanosoma brucei brucei
Function: Not known
Sequence: MPTRFKKTRHQRGSTFCGYGRVGKHRKHPSGRGNAGGEHHHRINFRKYHPGYFGKCGMNHYHKKKNTTWKPTINLDNLTKLMAKDEAMKAKKGEVLPVIDLLANGYSKLLGNGHLQAPCIVKARWVSKLADKKIRKAGGAVVLQA
InterPro domains: - IPR021131: Large ribosomal subunit protein uL15/eL18 (domain) [72-142]
- IPR030878: Large ribosomal subunit protein uL15 (family) [1-144]
- IPR036227: Large ribosomal subunit protein uL15/eL18 superfamily (homologous_superfamily) [6-143]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0019222 (regulation of metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0010468 (regulation of gene expression), GO:0010608 (post-transcriptional regulation of gene expression)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)
Protein: Large ribosomal subunit protein bL27 (50S ribosomal protein L27) (P9WHB3)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Not known
Sequence: MAHKKGASSSRNGRDSAAQRLGVKRYGGQVVKAGEILVRQRGTKFHPGVNVGRGGDDTLFAKTAGAVEFGIKRGRKTVSIVGSTTA
InterPro domains: - IPR018261: Large ribosomal subunit protein bL27, conserved site (conserved_site) [34-48]
- IPR001684: Large ribosomal subunit protein bL27 (family) [1-83]
Interaction partners: - Large ribosomal subunit protein bL17
- Large ribosomal subunit protein uL4
- Large ribosomal subunit protein uL13
- Large ribosomal subunit protein uL6
- Large ribosomal subunit protein uL30
- Large ribosomal subunit protein bL9
- Small ribosomal subunit protein uS3
- Large ribosomal subunit protein bL21
- Uncharacterized protein Rv0500B
- Large ribosomal subunit protein bL33B
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0044877 (protein-containing complex binding), GO:0005515 (protein binding), GO:0043021 (ribonucleoprotein complex binding), GO:0043022 (ribosome binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009605 (response to external stimulus), GO:0051707 (response to other organism), GO:0009607 (response to biotic stimulus), GO:0006950 (response to stress), GO:0006952 (defense response), GO:0009615 (response to virus), GO:0098542 (defense response to other organism), GO:0043207 (response to external biotic stimulus), GO:0140546 (defense response to symbiont), GO:0051607 (defense response to virus)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus)
Protein: Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial (EC 2.3.1.61) (2-oxoglutarate dehydrogenase complex component E2) (E2K) (Q9VGQ1)
Organism: Drosophila melanogaster (Fruit fly)
Function: Not known
Sequence: MTGIISIVTRRLPQTLGMRALRSNEVKRCIRQYSRLVACAAQQQQLLRQDGSNRCQEATRLLTWQGIHTTSSLWSEQTVNVPPFADSIAEGDIKFTCKVGDSFAADEAVMEIETDKTTVAVPAPFSGTLTDILVKDGDTVKPGQALFKIKPGAAPAKAAAPAAAPAPAAPKAAPAPAAAPKPAPPPPAAGAPKPPPPPPPKAAPRPPPPAPVAALKPAVAQVKVPPADGSRQILGTRSEQRVKMNRMRLKIAARLKDAQNTCAMLTTFNEVDMSYAMDFRKQNLDAFTKKYGIKFGFMSIFAKASAYALQDQPVVNAVIDGTDIVYRDYVDISVAVATPRGLVVPVIRNVEGMNYADIEIALAGLADKARRDAITVEDMDGGTFTISNGGVFGSLMGTPIINPPQSAILGMHGIFERPIAVKGEVKIRPMMYIALTYDHRIIDGREAVLFLRKIKAAVENPAIIVAGL
InterPro domains: - IPR000089: Biotin/lipoyl attachment (domain) [76-150]
- IPR006255: Dihydrolipoamide succinyltransferase (family) [78-464]
- IPR050537: 2-oxoacid dehydrogenase (family) [63-463]
- IPR001078: 2-oxoacid dehydrogenase acyltransferase, catalytic domain (domain) [238-463]
- IPR023213: Chloramphenicol acetyltransferase-like domain superfamily (homologous_superfamily) [226-466]
- IPR003016: 2-oxo acid dehydrogenase, lipoyl-binding site (binding_site) [100-129]
- IPR011053: Single hybrid motif (homologous_superfamily) [76-169]
Interaction partners: - Probable 2-oxoadipate dehydrogenase complex component E1 homolog
- 2-oxoglutarate dehydrogenase, mitochondrial
- Dihydrolipoyl dehydrogenase
- 2-oxoglutarate dehydrogenase, mitochondrial
- Succinate--CoA ligase subunit alpha, mitochondrial
- Succinate--CoA ligase subunit alpha, mitochondrial
- Succinate--CoA ligase subunit beta, mitochondrial
- Pyruvate dehydrogenase E1 component subunit beta, mitochondrial
- 2-oxoisovalerate dehydrogenase subunit alpha
- Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0044238 (primary metabolic process), GO:0006091 (generation of precursor metabolites and energy), GO:0006099 (tricarboxylic acid cycle), GO:0015980 (energy derivation by oxidation of organic compounds), GO:0045333 (cellular respiration), GO:0009060 (aerobic respiration)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098798 (mitochondrial protein-containing complex), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005739 (mitochondrion), GO:0043233 (organelle lumen), GO:0045239 (tricarboxylic acid cycle enzyme complex), GO:0043229 (intracellular organelle), GO:1990204 (oxidoreductase complex), GO:0043227 (membrane-bounded organelle), GO:0045240 (dihydrolipoyl dehydrogenase complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0005759 (mitochondrial matrix), GO:0070013 (intracellular organelle lumen)
Protein: RNA-binding protein, putative (Q382C7)
Organism: Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Function: Not known
Sequence: MQTCLLSNIPPCTEDELKRGLLAYGTTVSTVVVPHMRQAAVTMRTAQEAQALLQRGCISLFGQTVKVEPAASPAVPYGAGGISAGGMNNHLGGARINVVVEKCTYPVTKEVLSQVFSMVAPPANVVCGPSGPTTNGWVDYNDIATAQRVVVQLNNNSIYPDCCFMTLTLEHMGSTPRGMGEYGSQGHMPYHPQQPQHQNIHQNQQMSQQQLQAMPFGNNGFNAASARGGRGIRGRARGRGAMGGRGGPVYGSGYPMQHAPYAPGQYGAPPVDSVVIVNSVPETVALHSLWVLLEVYGNVNSLKRQFSSKTSVIAHFQNVHDARLAVQHLQGCPFHGATLTLRHFAGYVERGGKTEWNSGPATDPATQAVLFSCGYHHRTRPTASFNPTGRVRPGRYLFVSNLTEAITDDDLKDVFTNECFELDGYYRKSPTVAIVSLKDVETAVNALIAVHSHQLKERFLRVTFSHFPPAPRSGTEEEVEQALAATEAEAAEVREDVEPQDEQNE
InterPro domains: - IPR035979: RNA-binding domain superfamily (homologous_superfamily) [11-466]
- IPR012677: Nucleotide-binding alpha-beta plait domain superfamily (homologous_superfamily) [2-466]
- IPR000504: RNA recognition motif domain (domain) [3-467]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0003729 (mRNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0008380 (RNA splicing), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0000398 (mRNA splicing, via spliceosome), GO:0000375 (RNA splicing, via transesterification reactions), GO:0000365 (mRNA trans splicing, via spliceosome), GO:0000377 (RNA splicing, via transesterification reactions with bulged adenosine as nucleophile)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)
Protein: Testicular tissue protein Li 213 (A0A140VJY6)
Organism: Homo sapiens (Human)
Function: Not known
Sequence: MGQKGHKDSLYPCGGTPESSLHEALDQCMTALDLFLTNQFSEALSYLKPRTKESMYHSLTYATILEMQAMMTFDPQDILLAGNMMKEAQMLCQRHRRKSSVTDSFSSLVNRPTLGQFTEEEIHAEVCYAECLLQRAALTFLQDENMVSFIKGGIKVRNSYQTYKELDSLVQSSQYCKGENHPHFEGGVKLGVGAFNLTLSMLPTRILRLLEFVGFSGNKDYGLLQLEEGASGHSFRSVLCVMLLLCYHTFLTFVLGTGNVNIEEAEKLLKPYLNRYPKGAIFLFFAGRIEVIKGNIDAAIRRFEECCEAQQHWKQFHHMCYWELMWCFTYKGQWKMSYFYADLLSKENCWSKATYIYMKAAYLSMFGKEDHKPFGDDEVELFRAVPGLKLKIAGKSLPTEKFAIRKSRRYFSSNPISLPVPALEMMYIWNGYAVIGKQPKLTDGILEIITKAEEMLEKGPENEYSVDDECLVKLLKGLCLKYLGRVQEAEENFRSISANEKKIKYDHYLIPNALLELALLLMEQDRNEEAIKLLESAKQNYKNYSMESRTHFRIQAATLQAKSSLENSSRSMVSSVSL
InterPro domains: - IPR011990: Tetratricopeptide-like helical domain superfamily (homologous_superfamily) [258-551]
- IPR019412: IML2-like/Tetratricopeptide repeat protein 39-like (family) [18-561]
- IPR019734: Tetratricopeptide repeat (repeat) [280-544]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0009887 (animal organ morphogenesis), GO:0030154 (cell differentiation), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0022008 (neurogenesis), GO:0007399 (nervous system development), GO:0048880 (sensory system development), GO:0007423 (sensory organ development), GO:0090596 (sensory organ morphogenesis), GO:0030182 (neuron differentiation), GO:0048592 (eye morphogenesis), GO:0001654 (eye development), GO:0048699 (generation of neurons), GO:0046530 (photoreceptor cell differentiation), GO:0150063 (visual system development), GO:0043010 (camera-type eye development), GO:0048593 (camera-type eye morphogenesis), GO:0001754 (eye photoreceptor cell differentiation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005815 (microtubule organizing center), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0005813 (centrosome), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0015630 (microtubule cytoskeleton)
Protein: GH04611p (Q95SS0)
Organism: Drosophila melanogaster (Fruit fly)
Function: Not known
Sequence: MNAQRCRSVVPPVADPMCVEPLGVRNNTQLPPIPKILYFRRSRGHAVDLKIESSLLVYYFHVEVRSHIGRHFAARKLGPWQWQKVEKTMEENIGHSKHTYIFFHMRPGRWYEVRVAAVNAYGFRGYSEPSDPFPSTGNPKPPKSPNDSKIIGKQFDGRYMTLKLVWCPSKSNLPVEKYKITWSLYVNSAKASMITNSSYVKDTHQFEIKELLPNSSYYIQVQAISYLGSRRLKSEQWSMLFNTTLQPLEPITPLQCSGNGNRRRHHHTSSSMSSERATTSEPVALNEVSPTITNRTSAAATYEVGFRLNRKFGMIVQILGFQPHKEKVYELCPQETNCEQREFRAIRAKKDPLEFSKLKYNTTYVLRIPRSSPNSVLDDSRNVFTFTTPKCENFRKRFPKLQIKCSD
InterPro domains: - IPR042447: Anosmin-1 (family) [17-143]
- IPR036116: Fibronectin type III superfamily (homologous_superfamily) [95-229]
- IPR003961: Fibronectin type III (domain) [31-248]
- IPR013783: Immunoglobulin-like fold (homologous_superfamily) [52-250]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0044877 (protein-containing complex binding), GO:0005515 (protein binding), GO:0050839 (cell adhesion molecule binding), GO:0005178 (integrin binding), GO:0005102 (signaling receptor binding)
Biological Process (BP): GO:0008150 (biological process), GO:0040011 (locomotion), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0009605 (response to external stimulus), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0042221 (response to chemical), GO:0042330 (taxis), GO:0071840 (cellular component organization or biogenesis), GO:0032989 (cellular component morphogenesis), GO:0000902 (cell morphogenesis), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0048731 (system development), GO:0016043 (cellular component organization), GO:0006935 (chemotaxis), GO:0022008 (neurogenesis), GO:0000904 (cell morphogenesis involved in differentiation), GO:0030030 (cell projection organization), GO:0007399 (nervous system development), GO:0097485 (neuron projection guidance), GO:0032990 (cell part morphogenesis), GO:0048858 (cell projection morphogenesis), GO:0030182 (neuron differentiation), GO:0048666 (neuron development), GO:0048667 (cell morphogenesis involved in neuron differentiation), GO:0007411 (axon guidance), GO:0031175 (neuron projection development), GO:0048699 (generation of neurons), GO:0120039 (plasma membrane bounded cell projection morphogenesis), GO:0120036 (plasma membrane bounded cell projection organization), GO:0048812 (neuron projection morphogenesis), GO:0061564 (axon development), GO:0007409 (axonogenesis), GO:0008045 (motor neuron axon guidance)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0098552 (side of membrane), GO:0009986 (cell surface), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0009897 (external side of plasma membrane), GO:0005886 (plasma membrane)
Protein: 4-coumarate--CoA ligase 2 (4CL 2) (Os4CL2) (EC 6.2.1.12) ((E)-ferulate--CoA ligase) (EC 6.2.1.34) (4-coumaroyl-CoA synthase 2) (Q42982)
Organism: Oryza sativa subsp. japonica (Rice)
Function: Involved in the phenylpropanoid metabolism by mediating the activation of a number of hydroxycinnamates for the biosynthesis of monolignols and other phenolic secondary metabolites. Catalyzes the formation of CoA esters of cinnamate, 4-coumarate, caffeate and ferulate. Is more efficient with substrates in the following order: ferulate > 4-coumarate > caffeate > cinnamate. Cannot convert sinapate to its corresponding CoA ester. Follows a two-step reaction mechanism, wherein the carboxylate substrate first undergoes adenylation by ATP, followed by a thioesterification in the presence of CoA to yield the final CoA thioester (By similarity).
Sequence: MITVAAPEAQPQVAAAVDEAPPEAVTVFRSKLPDIDIPSHLPLHEYCFARAAELPDAPCLIAAATGRTYTFAETRLLCRRAAAALHRLGVGHGDRVMVLLQNCVEFAVAFFAASFLGAVTTAANPFCTPQEIHKQFKASGVKLILTQSVYVDKLRQHEAFPRIDACTVGDDTLTVITIDDDEATPEGCLPFWDLIADADEGSVPEVAISPDDPVALPFSSGTTGLPKGVVLTHRSVVSGVAQQVDGENPNLHMGAGDVALCVLPLFHIFSLNSVLLCAVRAGAAVALMPRFEMGAMLGAIERWRVTVAAVVPPLVLALAKNPFVERHDLSSIRIVLSGAAPLGKELEDALRARLPQAIFGQGYGMTEAGPVLSMCPAFAKEPTPAKSGSCGTVVRNAELKVVDPDTGFSLGRNLPGEICIRGPQIMKGYLNDPEATAATIDVEGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELESLLIAHPSIADAAVVPQKDDVAGEVPVAFVVRAADSDITEESIKEFISKQVVFYKRLHKVHFIHAIPKSASGKILRRELRAKLAAC
InterPro domains: - IPR042099: ANL, N-terminal domain (homologous_superfamily) [25-467]
- IPR000873: AMP-dependent synthetase/ligase domain (domain) [49-430]
- IPR045851: AMP-binding enzyme, C-terminal domain superfamily (homologous_superfamily) [468-568]
- IPR020845: AMP-binding, conserved site (conserved_site) [216-227]
- IPR025110: AMP-binding enzyme, C-terminal domain (domain) [481-556]
Interaction partners: - Phenylalanine ammonia-lyase
- tricin synthase
- Phenylalanine ammonia-lyase
- Chalcone synthase 1
- Aldehyde dehydrogenase 1
- Phenylalanine ammonia-lyase
- Os06g0592400 protein
- Hydroxycinnamoyltransferase 1
- Hydroxycinnamoyltransferase 2
- Aldehyde dehydrogenase 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0016877 (ligase activity, forming carbon-sulfur bonds), GO:0016878 (acid-thiol ligase activity), GO:0016405 (CoA-ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0009698 (phenylpropanoid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)