Protein: Subtilisin-like protease 1 (EC 3.4.21.62) (PfSUB1) (Q8I0V0)
Organism: Plasmodium falciparum (isolate 3D7)
Function: Serine protease which plays an essential role in merozoite invasion of and egress from host erythrocytes by processing and activating various merozoite surface and parasitophorous vacuole proteins. Mediates the proteolytic maturation of serine proteases SERA4, SERA5 and SERA6 just prior to merozoite egress. Prior to merozoite egress, cleaves merozoite surface proteins MSP1, MSP6 and MSP7, which form the MSP1/6/7 complex, and thereby may prime the parasite cell surface for invasion of fresh erythrocytes. Prior to merozoite egress, cleaves MSRP2 converting it to MSRP2 p25 form, and RAP1 converting it to RAP1 p67 form.
Subcellular location: Secreted {ECO:0000269|PubMed:18083098}. Parasitophorous vacuole lumen {ECO:0000269|PubMed:18083098}. Note=At the schizont stage, in merozoites, localizes to dense secretory granules called exonemes (PubMed:18083098). Just prior to egress secreted into the parasitophorous vacuole (PubMed:18083098). {ECO:0000269|PubMed:18083098}.
Sequence: MMLNKKVVALCTLTLHLFCIFLCLGKEVRSEENGKIQDDAKKIVSELRFLEKVEDVIEKSNIGGNEVDADENSFNPDTEVPIEEIEEIKMRELKDVKEEKNKNDNHNNNNNNISSSSSSSSNTFGEEKEEVSKKKKKLRLIVSENHATTPSFFQESLLEPDVLSFLESKGNLSNLKNINSMIIELKEDTTDDELISYIKILEEKGALIESDKLVSADNIDISGIKDAIRRGEENIDVNDYKSMLEVENDAEDYDKMFGMFNESHAATSKRKRHSTNERGYDTFSSPSYKTYSKSDYLYDDDNNNNNYYYSHSSNGHNSSSRNSSSSRSRPGKYHFNDEFRNLQWGLDLSRLDETQELINEHQVMSTRICVIDSGIDYNHPDLKDNIELNLKELHGRKGFDDDNNGIVDDIYGANFVNNSGNPMDDNYHGTHVSGIISAIGNNNIGVVGVDVNSKLIICKALDEHKLGRLGDMFKCLDYCISRNAHMINGSFSFDEYSGIFNSSVEYLQRKGILFFVSASNCSHPKSSTPDIRKCDLSINAKYPPILSTVYDNVISVANLKKNDNNNHYSLSINSFYSNKYCQLAAPGTNIYSTAPHNSYRKLNGTSMAAPHVAAIASLIFSINPDLSYKKVIQILKDSIVYLPSLKNMVAWAGYADINKAVNLAIKSKKTYINSNISNKWKKKSRYLH
InterPro domains: - IPR017314: Peptidase S8A, subtilisin-like peptidase 1, plasmodium (family) [2-686]
- IPR034204: Subtilisin SUB1-like catalytic domain (domain) [368-638]
- IPR015500: Peptidase S8, subtilisin-related (family) [363-619]
- IPR023827: Peptidase S8, subtilisin, Asp-active site (active_site) [368-379]
- IPR041089: SUB1 protease prodomain ProdP9 (domain) [138-217]
- IPR022398: Peptidase S8, subtilisin, His-active site (active_site) [428-438]
- IPR023828: Peptidase S8, subtilisin, Ser-active site (active_site) [604-614]
- IPR051048: Peptidase S8/S53 subtilisin kexin sedolisin (family) [310-622]
- IPR000209: Peptidase S8/S53 domain (domain) [366-639]
- IPR036852: Peptidase S8/S53 domain superfamily (homologous_superfamily) [328-671]
Interaction partners: - Subtilisin-like protease 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0017171 (serine hydrolase activity), GO:0008233 (peptidase activity), GO:0008236 (serine-type peptidase activity), GO:0004175 (endopeptidase activity), GO:0004252 (serine-type endopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0043170 (macromolecule metabolic process), GO:0051604 (protein maturation), GO:0006508 (proteolysis), GO:0010467 (gene expression), GO:0016485 (protein processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0045177 (apical part of cell), GO:0020007 (apical complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)