Protein: NAC domain containing protein 50 (ANAC050) (Q9SQX9)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Transcriptional repressor that binds to the motif 5'-(C/T)A(C/A)G-3' in the promoter of target genes. Binds also to the 5'-CTTGNNNNNCAAG-3' consensus sequence in chromatin. Can bind to the mitochondrial dysfunction motif (MDM) present in the upstream regions of mitochondrial dysfunction stimulon (MDS) genes involved in mitochondrial retrograde regulation (MRR). Together with NAC051/NAC052 and JMJ14, regulates gene expression and flowering time by associating with the histone demethylase JMJ14, probably by the promotion of RNA-mediated gene silencing.
Subcellular location: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353, ECO:0000269|PubMed:26617990}.
Sequence: MGRESLAVVSSPPSATAPSTAVSATSLAPGFRFHPTDEELVSYYLKRKVLGKPVRFDAIGEVDIYKHEPWDLAVFSKLKTRDQEWYFFSALDKKYGNGARMNRATNKGYWKATGKDREIRRDIQLLGMKKTLVFHSGRAPDGLRTNWVMHEYRLVEYETETNGSLLQDAYVLCRVFHKNNIGPPSGNRYAPFMEEEWADGGGALIPGIDVRVRVEALPQANGNNQMDQEMHSASKDLININELPRDATPMDIEPNQQNHHESAFKPQESNNHSGYEEDEDTLKREHAEEDERPPSLCILNKEAPLPLLQYKRRRQNESNNNSSRNTQDHCSSTITTVDNTTTLISSSAAAATNTAISALLEFSLMGISDKKENQQKEETSPPSPIASPEEKVNDLQKEVHQMSVERETFKLEMMSAEAMISILQSRIDALRQENEELKKKNASGQAS
InterPro domains: - IPR003441: NAC domain (domain) [27-178]
- IPR036093: NAC domain superfamily (homologous_superfamily) [20-181]
Interaction partners: - Lysine-specific demethylase JMJ14
- Leucine-rich repeat protein kinase family protein
- Sulfite exporter TauE/SafE family protein 1
- F-box/LRR-repeat protein At3g59250
- protein-synthesizing GTPase
- WPP domain-containing protein 3
- Protein GAMETE EXPRESSED 3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:1901363 (heterocyclic compound binding), GO:0003700 (DNA-binding transcription factor activity), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0001067 (transcription regulatory region nucleic acid binding), GO:0003677 (DNA binding), GO:0003690 (double-stranded DNA binding), GO:0043565 (sequence-specific DNA binding), GO:0000976 (transcription cis-regulatory region binding), GO:1990837 (sequence-specific double-stranded DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0022414 (reproductive process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0009628 (response to abiotic stimulus), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009058 (biosynthetic process), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0003006 (developmental process involved in reproduction), GO:0009892 (negative regulation of metabolic process), GO:0009791 (post-embryonic development), GO:0048573 (photoperiodism, flowering), GO:0044237 (cellular metabolic process), GO:0009607 (response to biotic stimulus), GO:0050794 (regulation of cellular process), GO:0048523 (negative regulation of cellular process), GO:0051707 (response to other organism), GO:0044238 (primary metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0043207 (response to external biotic stimulus), GO:1901576 (organic substance biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0009314 (response to radiation), GO:0048731 (system development), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0048608 (reproductive structure development), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0048229 (gametophyte development), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009617 (response to bacterium), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0016043 (cellular component organization), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0010228 (vegetative to reproductive phase transition of meristem), GO:0061458 (reproductive system development), GO:0009416 (response to light stimulus), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0009555 (pollen development), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0002237 (response to molecule of bacterial origin), GO:0006325 (chromatin organization), GO:0010468 (regulation of gene expression), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0051253 (negative regulation of RNA metabolic process), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0010467 (gene expression), GO:0010629 (negative regulation of gene expression), GO:0009648 (photoperiodism), GO:0016070 (RNA metabolic process), GO:0060966 (regulation of gene silencing by RNA), GO:2001141 (regulation of RNA biosynthetic process), GO:0016441 (post-transcriptional gene silencing), GO:0031047 (RNA-mediated gene silencing), GO:0006355 (regulation of DNA-templated transcription), GO:0006396 (RNA processing), GO:0040029 (epigenetic regulation of gene expression), GO:0045814 (negative regulation of gene expression, epigenetic), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0010608 (post-transcriptional regulation of gene expression), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0034470 (ncRNA processing), GO:0070918 (regulatory ncRNA processing), GO:0045892 (negative regulation of DNA-templated transcription), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0006351 (DNA-templated transcription), GO:0034660 (ncRNA metabolic process), GO:0000122 (negative regulation of transcription by RNA polymerase II), GO:0030422 (siRNA processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)