Protein: Caspase-3 (CASP-3) (EC 3.4.22.56) (Apopain) (Cysteine protease CPP32) (CPP-32) (LICE) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12] (P70677)
Organism: Mus musculus (Mouse)
Function: Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes (By similarity). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction. Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (By similarity). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface.
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:P42574}.
Sequence: MENNKTSVDSKSINNFEVKTIHGSKSVDSGIYLDSSYKMDYPEMGICIIINNKNFHKSTGMSSRSGTDVDAANLRETFMGLKYQVRNKNDLTREDILELMDSVSKEDHSKRSSFVCVILSHGDEGVIYGTNGPVELKKLTSFFRGDYCRSLTGKPKLFIIQACRGTELDCGIETDSGTDEEMACQKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCSMLKLYAHKLEFMHILTRVNRKVATEFESFSLDSTFHAKKQIPCIVSMLTKELYFYH
InterPro domains: - IPR011600: Peptidase C14, caspase domain (domain) [44-274]
- IPR015917: Peptidase C14A, caspase catalytic domain (domain) [36-277]
- IPR016129: Peptidase family C14A, His active site (active_site) [108-122]
- IPR002398: Peptidase C14 family (family) [33-275]
- IPR002138: Peptidase C14, caspase non-catalytic subunit p10 (domain) [183-277]
- IPR033139: Peptidase family C14A, cysteine active site (active_site) [154-165]
- IPR029030: Caspase-like domain superfamily (homologous_superfamily) [36-276]
- IPR001309: Peptidase C14, p20 domain (domain) [43-167]
Interaction partners: - E3 ubiquitin-protein ligase XIAP
- Caspase-9
- Caspase-8
- Apoptotic protease-activating factor 1
- Poly [ADP-ribose] polymerase 1
- Cytochrome c, somatic
- Cytochrome c, testis-specific
- Baculoviral IAP repeat-containing protein 3
- Actin, cytoplasmic 1
- Granzyme B
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0008233 (peptidase activity), GO:0008234 (cysteine-type peptidase activity), GO:0004175 (endopeptidase activity), GO:0070001 (aspartic-type peptidase activity), GO:0004197 (cysteine-type endopeptidase activity), GO:0004190 (aspartic-type endopeptidase activity), GO:0097153 (cysteine-type endopeptidase activity involved in apoptotic process)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0008152 (metabolic process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0022414 (reproductive process), GO:0009605 (response to external stimulus), GO:0019953 (sexual reproduction), GO:0048856 (anatomical structure development), GO:0042221 (response to chemical), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0032504 (multicellular organism reproduction), GO:0003006 (developmental process involved in reproduction), GO:0065008 (regulation of biological quality), GO:0009607 (response to biotic stimulus), GO:0008219 (cell death), GO:0050794 (regulation of cellular process), GO:0008037 (cell recognition), GO:0051716 (cellular response to stimulus), GO:0051707 (response to other organism), GO:0007165 (signal transduction), GO:0048869 (cellular developmental process), GO:0009628 (response to abiotic stimulus), GO:0007275 (multicellular organism development), GO:0019222 (regulation of metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0003008 (system process), GO:0009893 (positive regulation of metabolic process), GO:0006950 (response to stress), GO:0048609 (multicellular organismal reproductive process), GO:0007154 (cell communication), GO:0044238 (primary metabolic process), GO:0007610 (behavior), GO:0048522 (positive regulation of cellular process), GO:0001101 (response to acid chemical), GO:0012501 (programmed cell death), GO:0043207 (response to external biotic stimulus), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0009314 (response to radiation), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0060255 (regulation of macromolecule metabolic process), GO:0048608 (reproductive structure development), GO:0045137 (development of primary sexual characteristics), GO:0003013 (circulatory system process), GO:0007626 (locomotory behavior), GO:0010035 (response to inorganic substance), GO:0007611 (learning or memory), GO:0030900 (forebrain development), GO:0097190 (apoptotic signaling pathway), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0021543 (pallium development), GO:0016043 (cellular component organization), GO:0007166 (cell surface receptor signaling pathway), GO:0007548 (sex differentiation), GO:1901575 (organic substance catabolic process), GO:0035094 (response to nicotine), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0007276 (gamete generation), GO:0007281 (germ cell development), GO:1901564 (organonitrogen compound metabolic process), GO:0035556 (intracellular signal transduction), GO:0009611 (response to wounding), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0019538 (protein metabolic process), GO:0048515 (spermatid differentiation), GO:1901700 (response to oxygen-containing compound), GO:0031647 (regulation of protein stability), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0007283 (spermatogenesis), GO:0010941 (regulation of cell death), GO:1901698 (response to nitrogen compound), GO:0060322 (head development), GO:0070887 (cellular response to chemical stimulus), GO:0009617 (response to bacterium), GO:0046677 (response to antibiotic), GO:0050877 (nervous system process), GO:0080090 (regulation of primary metabolic process), GO:0030030 (cell projection organization), GO:0030163 (protein catabolic process), GO:0048545 (response to steroid hormone), GO:0008406 (gonad development), GO:0002237 (response to molecule of bacterial origin), GO:0048666 (neuron development), GO:0007420 (brain development), GO:0009057 (macromolecule catabolic process), GO:0007399 (nervous system development), GO:0007417 (central nervous system development), GO:0097193 (intrinsic apoptotic signaling pathway), GO:0010212 (response to ionizing radiation), GO:0010038 (response to metal ion), GO:0050890 (cognition), GO:0009416 (response to light stimulus), GO:0034097 (response to cytokine), GO:0032496 (response to lipopolysaccharide), GO:0043200 (response to amino acid), GO:0007413 (axonal fasciculation), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0034250 (positive regulation of amide metabolic process), GO:0071310 (cellular response to organic substance), GO:1901214 (regulation of neuron death), GO:0022008 (neurogenesis), GO:0042060 (wound healing), GO:0034248 (regulation of amide metabolic process), GO:0010243 (response to organonitrogen compound), GO:0046545 (development of primary female sexual characteristics), GO:0071241 (cellular response to inorganic substance), GO:0033993 (response to lipid), GO:0006915 (apoptotic process), GO:0051247 (positive regulation of protein metabolic process), GO:0014070 (response to organic cyclic compound), GO:0006996 (organelle organization), GO:0048232 (male gamete generation), GO:0030182 (neuron differentiation), GO:0042981 (regulation of apoptotic process), GO:0008585 (female gonad development), GO:0032025 (response to cobalt ion), GO:0031960 (response to corticosteroid), GO:0043523 (regulation of neuron apoptotic process), GO:0071345 (cellular response to cytokine stimulus), GO:0010154 (fruit development), GO:0048699 (generation of neurons), GO:0043525 (positive regulation of neuron apoptotic process), GO:0043065 (positive regulation of apoptotic process), GO:1902003 (regulation of amyloid-beta formation), GO:0120036 (plasma membrane bounded cell projection organization), GO:0034284 (response to monosaccharide), GO:0071407 (cellular response to organic cyclic compound), GO:0051146 (striated muscle cell differentiation), GO:0031175 (neuron projection development), GO:0071887 (leukocyte apoptotic process), GO:0010165 (response to X-ray), GO:0008306 (associative learning), GO:0048812 (neuron projection morphogenesis), GO:0061564 (axon development), GO:0051384 (response to glucocorticoid), GO:0007605 (sensory perception of sound), GO:0009746 (response to hexose), GO:0009749 (response to glucose)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:1990904 (ribonucleoprotein complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0044297 (cell body), GO:0005829 (cytosol), GO:0036477 (somatodendritic compartment), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0043209 (myelin sheath), GO:0043025 (neuronal cell body), GO:0005739 (mitochondrion), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)