Protein: Chromatin assembly factor 1 subunit A (CAF-1 subunit A) (Chromatin assembly factor I p150 subunit) (CAF-I 150 kDa subunit) (CAF-I p150) (hp150) (Q13111)
Organism: Homo sapiens (Human)
Function: Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.
Subcellular location: Nucleus {ECO:0000269|PubMed:10052459}. Note=DNA replication foci.
Sequence: MLEELECGAPGARGAATAMDCKDRPAFPVKKLIQARLPFKRLNLVPKGKADDMSDDQGTSVQSKSPDLEASLDTLENNCHVGSDIDFRPKLVNGKGPLDNFLRNRIETSIGQSTVIIDLTEDSNEQPDSLVDHNKLNSEASPSREAINGQREDTGDQQGLLKAIQNDKLAFPGETLSDIPCKTEEEGVGCGGAGRRGDSQECSPRSCPELTSGPRMCPRKEQDSWSEAGGILFKGKVPMVVLQDILAVRPPQIKSLPATPQGKNMTPESEVLESFPEEDSVLSHSSLSSPSSTSSPEGPPAPPKQHSSTSPFPTSTPLRRITKKFVKGSTEKNKLRLQRDQERLGKQLKLRAEREEKEKLKEEAKRAKEEAKKKKEEEKELKEKERREKREKDEKEKAEKQRLKEERRKERQEALEAKLEEKRKKEEEKRLREEEKRIKAEKAEITRFFQKPKTPQAPKTLAGSCGKFAPFEIKEHMVLAPRRRTAFHPDLCSQLDQLLQQQSGEFSFLKDLKGRQPLRSGPTHVSTRNADIFNSDVVIVERGKGDGVPERRKFGRMKLLQFCENHRPAYWGTWNKKTALIRARDPWAQDTKLLDYEVDSDEEWEEEEPGESLSHSEGDDDDDMGEDEDEDDGFFVPHGYLSEDEGVTEECADPENHKVRQKLKAKEWDEFLAKGKRFRVLQPVKIGCVWAADRDCAGDDLKVLQQFAACFLETLPAQEEQTPKASKRERRDEQILAQLLPLLHGNVNGSKVIIREFQEHCRRGLLSNHTGSPRSPSTTYLHTPTPSEDAAIPSKSRLKRLISENSVYEKRPDFRMCWYVHPQVLQSFQQEHLPVPCQWSYVTSVPSAPKEDSGSVPSTGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQIGAEDMDGFQADTEEEEEEEGDCMIVDVPDAAEVQAPCGAASGAGGGVGVDTGKATLTASPLGAS
InterPro domains: - IPR022043: Chromatin assembly factor 1 subunit A, dimerization domain (domain) [558-629]
- IPR029091: Chromatin assembly factor 1 subunit p150, N-terminal (domain) [1-226]
- IPR021644: Chromatin assembly factor 1 p150 subunit, acidic region (domain) [340-478]
- IPR029105: Chromatin assembly factor 1 subunit p150, C-terminal (domain) [665-954]
Interaction partners: - Histone-binding protein RBBP4
- Chromatin assembly factor 1 subunit B
- Chromobox protein homolog 5
- Histone H3.1
- Histone chaperone ASF1B
- Proliferating cell nuclear antigen
- DNA replication licensing factor MCM5
- Chromobox protein homolog 3
- Histone H4
- Histone chaperone ASF1A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0009892 (negative regulation of metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0065009 (regulation of molecular function), GO:0006950 (response to stress), GO:0048523 (negative regulation of cellular process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0050790 (regulation of catalytic activity), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0044093 (positive regulation of molecular function), GO:0046483 (heterocycle metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0033554 (cellular response to stress), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0090398 (cellular senescence), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0016043 (cellular component organization), GO:0080090 (regulation of primary metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051052 (regulation of DNA metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0051054 (positive regulation of DNA metabolic process), GO:0006325 (chromatin organization), GO:0010468 (regulation of gene expression), GO:0051053 (negative regulation of DNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0006259 (DNA metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0051338 (regulation of transferase activity), GO:0043085 (positive regulation of catalytic activity), GO:0006996 (organelle organization), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0051347 (positive regulation of transferase activity), GO:2001141 (regulation of RNA biosynthetic process), GO:0000723 (telomere maintenance), GO:0006310 (DNA recombination), GO:0006281 (DNA repair), GO:0051276 (chromosome organization), GO:0045005 (DNA-templated DNA replication maintenance of fidelity), GO:0006260 (DNA replication), GO:0006275 (regulation of DNA replication), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0008156 (negative regulation of DNA replication), GO:0006261 (DNA-templated DNA replication), GO:0000725 (recombinational repair), GO:0006289 (nucleotide-excision repair), GO:0045892 (negative regulation of DNA-templated transcription), GO:0090329 (regulation of DNA-templated DNA replication), GO:0032200 (telomere organization), GO:0006312 (mitotic recombination), GO:0036297 (interstrand cross-link repair), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0031297 (replication fork processing), GO:0006302 (double-strand break repair), GO:0000724 (double-strand break repair via homologous recombination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0032993 (protein-DNA complex), GO:0000785 (chromatin), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005694 (chromosome), GO:0005634 (nucleus)