Protein: Probable ATP-dependent RNA helicase spindle-E (EC 3.6.4.13) (Protein homeless) (Q9VF26)
Organism: Drosophila melanogaster (Fruit fly)
Function: Probable ATP-binding RNA helicase which plays a central role during spermatogenesis and oogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi and govern the methylation and subsequent repression of transposons. Involved in the repression of LTR retrotransposon copia. Also involved in telomere regulation by repressing specialized telomeric retroelements HeT-A, TAHRE, and TART; Drosophila telomeres being maintained by transposition of specialized telomeric retroelements. Involved in telomeric trans-silencing, a repression mechanism by which a transposon or a transgene inserted in subtelomeric heterochromatin has the capacity to repress in trans in the female germline, a homologous transposon, or transgene located in euchromatin. Involved in the repression of testis-expressed Stellate genes by the homologous Su(Ste) repeats. Required for anteroposterior and dorsoventral axis formation during oogenesis.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:26295961}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:26295961}. Cytoplasm, Cytoplasmic ribonucleoprotein granule {ECO:0000269|PubMed:26295961}. Note=Component of the perinuclear meiotic nuage (also known as germline granule or P granule), a germline-specific membraneless ribonucleoprotein biocondensate involved in post-transcriptional regulation of gene expression. {ECO:0000269|PubMed:26295961}.
Sequence: MDQEVMDFFDFSKELKRVAAAPQGYISSDPRLMATKFKSSEVPNRELIGTDYVSKIVAKEKCLLNGTLLNEQPQGKRIRTLDDLDTDDEGEETEIRRDDEYYKKFRFNLNRDKNLSIYAKREEILAAINAHPVVIIKGETGCGKTTQVPQYILDEAYKSGKYCNIVVTQPRRIAAISIANRVCQEREWQQNTVCSFQVGLHRPNSLEDTRLLYCTTGVLLNNLINNKTLTHYTHIVLDEVHERDQNMDFLLIVVRRLLATNSRHVKIILMSATIDAKELSDYFTTTNSIPPVITTNHRRKHSIEKFYRDQLGSIIWNEEDVGHQQVPEINKHGYRAAVKIIVIIDNMERKAAIQSRQSYDEALRYGAVLIFLPGIYEIDTMAENLTCMLENDPNIKVSIVRCFSLMTPENQRDVFNPPPPGFRKIILTTNIAESSITVPDVSYVIDFCLAKVKVTDTASSFSSLRLTWASKANCRQRAGRVGRLRSGRVYRMVNKHFYQREMPEFGIPEMLRLPLQNSVLKAKVLNMGSPVEILALALSPPNLSDIHNTILLLKEVGALYLTVDGIYDPLDGDLTYWGTIMSRLPLDTRQSRLIILGYIFNMLEEAIIIAAGLSTPGLFAHEGGRSQLGDSFWMHYIFSDGSGSDLVAIWRVYLTYLNIVENGHDQESAIRWAKRFHVSLRSLKEIHLLVQELRVRCTHLGLIPFPVNPNQMMDDREKAIMLKVIIAGAFYPNYFTRSKESCADTDRNIYQTISGHDPCRTVYFTNFKPAYMGELYTRRIKELFQEVRIPPENMDVTFQEGSQKVFVTFKQDDWIEGSSKYVPVSGRVQSEVYKAVMMRQNRVERPIHIMNPSAFMSYVQQRGIGDVIEGRWIPPTKPLNVELLALPSVFDKTISGSITCIVNCGKFFFQPQSFEECIRNMSEIFNAPQQLRNYVTNASAIAKGMMVLAKRDSYFQRATVIRPENQSNRQPMFYVRFIDYGNCTLLPMQLMRLMPRELTEQYGDLPPRVFECRLAMVQPSSVVSGNNRWSTAANDMLKTVAQCGLIDIEVYSLFNNVAAVLIHMRDGIINDKLVELMLCRRSDEDYMSRKDHDFRLRRQESARNLSTAQRQQINEEYLRSCQLPQDHDLPPPPLEKCKTVVMLKGPNSPLECTMRSITRVGLSKRVNIDHLSVNALLLDADPQDHHDHLIVAHEIAESRNGQTLTARGTTLMPNVQGFGALMVMLFSPTMQLKCNKEGTSYVSVLGGLGCDPDTNEPYFAEHDVLINLDVNILEDDVILINQIRYYIDSVFFNFKEENNPAVSVNERVSIYTQLRSLINRLLCKDRRYIERNMSNADFEWETNPELPLPNEPFGKRAIFPMHSLTELQEEDTGRLVQLRENCSMLHKWRNFEGTLPHMTCKLCNQLLESVPQLRLHLLTILHRDREKQIDYCNQ
InterPro domains: - IPR035437: SNase-like, OB-fold superfamily (homologous_superfamily) [898-1080]
- IPR013087: Zinc finger C2H2-type (domain) [1400-1422]
- IPR007502: Helicase-associated domain (domain) [548-650]
- IPR002999: Tudor domain (domain) [891-1015]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [69-697]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [113-302]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [124-279]
- IPR001650: Helicase, C-terminal domain-like (domain) [354-526]
Interaction partners: - Protein piwi
- Protein aubergine
- Tudor domain-containing protein krimp
- Probable RNA helicase armi
- Protein vreteno
- Mitochondrial cardiolipin hydrolase
- Qin
- FI02030p
- Protein argonaute-3
- Putative ATP-dependent RNA helicase BoYb
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0004386 (helicase activity), GO:1901363 (heterocyclic compound binding), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:0008186 (ATP-dependent activity, acting on RNA), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0140098 (catalytic activity, acting on RNA), GO:0003724 (RNA helicase activity), GO:0003723 (RNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0022414 (reproductive process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0019953 (sexual reproduction), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0022412 (cellular process involved in reproduction in multicellular organism), GO:0051321 (meiotic cell cycle), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:0071840 (cellular component organization or biogenesis), GO:1903046 (meiotic cell cycle process), GO:0032504 (multicellular organism reproduction), GO:0003006 (developmental process involved in reproduction), GO:0048609 (multicellular organismal reproductive process), GO:0061982 (meiosis I cell cycle process), GO:0007276 (gamete generation), GO:0007281 (germ cell development), GO:0140013 (meiotic nuclear division), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0048515 (spermatid differentiation), GO:0016043 (cellular component organization), GO:0007140 (male meiotic nuclear division), GO:0007283 (spermatogenesis), GO:0007141 (male meiosis I), GO:0007286 (spermatid development), GO:0006996 (organelle organization), GO:0048232 (male gamete generation), GO:0007127 (meiosis I), GO:0048285 (organelle fission), GO:0000280 (nuclear division)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043229 (intracellular organelle), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)