Protein: Negative elongation factor E (P92204)
Organism: Drosophila melanogaster (Fruit fly)
Function: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex, causes transcriptional pausing.
Subcellular location: Nucleus {ECO:0000269|PubMed:12782658}. Chromosome {ECO:0000269|PubMed:12782658}. Note=Associates with polytene chromosomes. Associates with the hsp70 promoter when it is inactive, but not when it is activated. The NELF complex possibly dissociates from chromatin following phosphorylation of RNA polymerase II.
Sequence: MVYIHFPNNLTEEEQMLQAKYQKLKKKKKALQAHKAPKPEPESSLTLKRPTDARDAREVARKLIKSGAIPAIQKQTKQDQTSFKRPKGQERAKRSTSETTVASYQPFSSTQNDVAQETIISEIIKEEPRRQNLYQHFATERDREERGMPEKVPMDTAQPEKPRAGNTIFVSGNKVTEDFLKKTFNDYGTIVNVSMEIEKSRGFVSFAKPESADRAIAEIHGKNVNGINLQVQLARRQPQIEPINDASSSAVWSSIAASKSQKGSHKDHREMVQYDEDFLL
InterPro domains: - IPR034637: Negative elongation factor E, RNA recognition motif (domain) [162-236]
- IPR035979: RNA-binding domain superfamily (homologous_superfamily) [146-241]
- IPR012677: Nucleotide-binding alpha-beta plait domain superfamily (homologous_superfamily) [161-246]
- IPR033102: Negative elongation factor E (family) [1-278]
- IPR000504: RNA recognition motif domain (domain) [166-236]
Interaction partners: - Negative elongation factor B
- Negative elongation factor D
- Negative elongation factor A
- Transcription elongation factor SPT5
- Transcription elongation factor SPT4
- Nuclear cap-binding protein subunit 2
- DNA-directed RNA polymerase II subunit RPB3
- Cyclin-dependent kinase 9
- Nuclear cap-binding protein subunit 1
- DNA-directed RNA polymerase II subunit RPB1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0017069 (snRNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0019953 (sexual reproduction), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0022412 (cellular process involved in reproduction in multicellular organism), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0032504 (multicellular organism reproduction), GO:0003006 (developmental process involved in reproduction), GO:0048609 (multicellular organismal reproductive process), GO:0048522 (positive regulation of cellular process), GO:0007276 (gamete generation), GO:0007281 (germ cell development), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0048468 (cell development), GO:0031325 (positive regulation of cellular metabolic process), GO:0030154 (cell differentiation), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:0007283 (spermatogenesis), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:0048232 (male gamete generation), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0010468 (regulation of gene expression), GO:1902680 (positive regulation of RNA biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0032784 (regulation of DNA-templated transcription elongation), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0034243 (regulation of transcription elongation by RNA polymerase II), GO:0032786 (positive regulation of DNA-templated transcription, elongation), GO:0045944 (positive regulation of transcription by RNA polymerase II), GO:0032968 (positive regulation of transcription elongation by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:1990904 (ribonucleoprotein complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140513 (nuclear protein-containing complex), GO:0120114 (Sm-like protein family complex), GO:0030532 (small nuclear ribonucleoprotein complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0097525 (spliceosomal snRNP complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)