Protein: Actin-regulating kinase PRK1 (EC 2.7.11.1) (p53-regulating kinase 1) (P40494)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Protein kinase involved in the regulation of actin cytoskeleton organization and endocytosis. Phosphorylates PAN1 which disrupts the interaction between PAN1 and END3, and between PAN1 and SLA1. Phosphorylates SCD5. Preferentially, phosphorylates substrates on threonine residues in a [L/I/V/M]-x-x-[Q/N/T/S]-x-T-G motif.
Subcellular location: Cytoplasm, cytoskeleton, actin patch {ECO:0000269|PubMed:10087264, ECO:0000269|PubMed:14562095}. Note=Cortical actin patches.
Sequence: MNTPQISLYEPGTILTVGSHHAKIIKYLTSGGFAQVYTAEISPPDPYSNANIACLKRVIVPHKQGLNTLRAEVDAMKLLRNNKHVVSYIDSHAARSVNGIAYEVFVLMEFCERGGLIDFMNTRLQNRLQESEILEIMSQTVQGITAMHALQPPLIHRDIKIENVLISHDGLYKVCDFGSVSGVIRPPRNTQEFNYVQHDILTNTTAQYRSPEMIDLYRGLPIDEKSDIWALGVFLYKICYYTTPFEKSGEAGILHARYQYPSFPQYSDRLKNLIRLMLMEAPSQRPNICQVLEEVSRLQNKPCPIRNFYLLRAMNQNANTQLAGEPSSTTYVPTQKFIPVQSLQSINQPPNMMPVTHVSTTPNLGTFPISINDNNKTEVTAHAGLQVGSHSNLTSPLMKTKSVPLSDEFASLYYKELHPFQKSQTFKSVESFQSPQRKSMPPLSLTPVNNDIFDRVSAINRPNNYVDSETQTIDNMAVPNLKLSPTITSKSLSSTKEIAAPDNINGSKIVRSLSSKLKKVITGESRGNSPIKSRQNTGDSIRSAFGKLRHGFTGNSVNNSRSASFDNNNVNGNGNNTNRRLVSSSTSSFPKFNSDTKRKEESDKNQRLEKRRSMPPSILSDFDQHERNNSRTGSRDYYRSHSPVKKTQASAKTTSKPTLIPDNGNVNINQEKKESIQRRVHNLLKSSDDPVTYKSASGYGKYTDIGTETSNRHSSVRITPITEEKFKKTLKDGVLDIKTKSNGKDKSRPPRPPPKPLHLRTEIQKIRNFSRLQSKKLPIERISSEATETIVDVNVDDLEADFRKRFPSKV
InterPro domains: - IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [16-350]
- IPR008271: Serine/threonine-protein kinase, active site (active_site) [154-166]
- IPR000719: Protein kinase domain (domain) [22-298]
Interaction partners: - Actin-binding protein
- Actin cytoskeleton-regulatory complex protein PAN1
- Actin cytoskeleton-regulatory complex protein SLA1
- Protein SCD5
- Protein SLA2
- Clathrin coat assembly protein AP180A
- Proline-rich protein LAS17
- Actin cytoskeleton-regulatory complex protein END3
- Epsin-1
- Actin-regulating kinase 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0051641 (cellular localization), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:0071840 (cellular component organization or biogenesis), GO:0009056 (catabolic process), GO:0033036 (macromolecule localization), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0030029 (actin filament-based process), GO:0006950 (response to stress), GO:0061919 (process utilizing autophagic mechanism), GO:0048523 (negative regulation of cellular process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044085 (cellular component biogenesis), GO:1903047 (mitotic cell cycle process), GO:0035556 (intracellular signal transduction), GO:0044248 (cellular catabolic process), GO:0019538 (protein metabolic process), GO:0000226 (microtubule cytoskeleton organization), GO:0010948 (negative regulation of cell cycle process), GO:0030036 (actin cytoskeleton organization), GO:0043170 (macromolecule metabolic process), GO:0006914 (autophagy), GO:0006793 (phosphorus metabolic process), GO:0051726 (regulation of cell cycle), GO:0007059 (chromosome segregation), GO:0033554 (cellular response to stress), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0045786 (negative regulation of cell cycle), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0010564 (regulation of cell cycle process), GO:0000278 (mitotic cell cycle), GO:0098813 (nuclear chromosome segregation), GO:0007346 (regulation of mitotic cell cycle), GO:0000075 (cell cycle checkpoint signaling), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0006974 (cellular response to DNA damage stimulus), GO:0042770 (signal transduction in response to DNA damage), GO:0019439 (aromatic compound catabolic process), GO:0140014 (mitotic nuclear division), GO:0046700 (heterocycle catabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0007093 (mitotic cell cycle checkpoint signaling), GO:0022607 (cellular component assembly), GO:0016236 (macroautophagy), GO:0009057 (macromolecule catabolic process), GO:0051983 (regulation of chromosome segregation), GO:0044265 (cellular macromolecule catabolic process), GO:1901987 (regulation of cell cycle phase transition), GO:0045930 (negative regulation of mitotic cell cycle), GO:1905819 (negative regulation of chromosome separation), GO:0031134 (sister chromatid biorientation), GO:0033043 (regulation of organelle organization), GO:0043933 (protein-containing complex organization), GO:0008104 (protein localization), GO:0006996 (organelle organization), GO:0000070 (mitotic sister chromatid segregation), GO:1901988 (negative regulation of cell cycle phase transition), GO:0070925 (organelle assembly), GO:0032272 (negative regulation of protein polymerization), GO:0033365 (protein localization to organelle), GO:0051784 (negative regulation of nuclear division), GO:0010965 (regulation of mitotic sister chromatid separation), GO:0033045 (regulation of sister chromatid segregation), GO:1901991 (negative regulation of mitotic cell cycle phase transition), GO:0065003 (protein-containing complex assembly), GO:0006468 (protein phosphorylation), GO:0048285 (organelle fission), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0051603 (proteolysis involved in protein catabolic process), GO:0051276 (chromosome organization), GO:0051603 (proteolysis involved in protein catabolic process), GO:0007010 (cytoskeleton organization), GO:0033048 (negative regulation of mitotic sister chromatid segregation), GO:0031577 (spindle checkpoint signaling), GO:0045841 (negative regulation of mitotic metaphase/anaphase transition), GO:0033047 (regulation of mitotic sister chromatid segregation), GO:1901990 (regulation of mitotic cell cycle phase transition), GO:0000819 (sister chromatid segregation), GO:1902100 (negative regulation of metaphase/anaphase transition of cell cycle), GO:2001251 (negative regulation of chromosome organization), GO:0033044 (regulation of chromosome organization), GO:0071174 (mitotic spindle checkpoint signaling), GO:0061912 (selective autophagy), GO:0000280 (nuclear division), GO:0007094 (mitotic spindle assembly checkpoint signaling), GO:0051258 (protein polymerization), GO:0140694 (non-membrane-bounded organelle assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)