Protein: Probable DNA-directed RNA polymerase I subunit RPA2 (Q9P7X8)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity)
Subcellular location: Nucleus, nucleolus
Sequence: MSFQTLERERTFKNPPKDGTSFPDLQKAVKPHVDSFNALTNAGLLNYAVKEIGEKCAFDSITQEEGGALKFGNKISFRVDEVQIAKPMLSSRERSSINRKVYPAEARERLTTYKSRLVLKFSWSVNGGPRQSEMREVGMIPIMVRSNRCHLEGLSPAELIAHKEESEEMGGYFIVNGIEKLIRMLILPKRNHPTAIIRPSFANRGTSYSQYGLSIRCVRPDQSSLTNTLHYLNNGVTMFRFHWRKNEYLIPSMMILKALLETSDKEIFEGIVGKDLGNTFLTDRVELMLRAYKSYGLYSQTETLQYLGSKFRVVLGVAEDLTDVEVGRFLLQKVVLVHLREAKDKFRLLLFMIRKLYALVAGECCADNPDSPQHQEILLGGFLYGQILKEKIEDWLNSIRAQINLDVRRSAPGVDFSDRKYLTRVFSKINNDIGTKLQYFLSTGNLVSNTGLDLQQATGYTVVAEKLNFYRFLSHFRMVHRGAFFAELKTTTVRKLLPEAWGFMCPVHTPDGSPCGLLNHLARKCEIVTHPSDVSQIPSLLLSLGVDPPSVVGHESGWGCVQLDGKIVGWCTYKLAKHVADVLRLMKIEYAVKLRNGTATEPAKVPLDLEIGYVPPSHNGQYPGLYLFSNPARMVRPVKHISTGELDMLGPFEQVYMDIACFPKEIVPKVSTHVEYSPTNVLSIVANMTPFSDFNQSPRNMYQCQMGKQTMGTPGTALRYRTDNKLYRLQTGQTPVVRPKLHNTYGLDHYPNGTNAVVAVISYTGYDMEDAMILNKSAHERGFGYGTVYKGESFDLSQKRRRGEPVVHHFGFAPGSTPRREWLQKLDADGLPFIGIKLEDGDPIVAYYDESTGQNFIETYHGTEPGFVDEVRLLGNDVGDSECQQIHVKLRITRSPIIGDKFSSRHGQKGICSQKWPTVDMPFTESGMQPDIIINPHAFPSRMTIGMFIESLAGKAGACHGLAQDSTPFIYSEQQTAADYFGEQLVKAGYNYHGNEPMYSGITGQEMKADIYIGVVYYQRLRHMVSDKFQVRTTGPIHNLTRQPVKGRKRAGGIRFGEMERDAVIGHGTSFLMQDRLMNCSDYAQSWVCRDCGSIISIMSTISMNGVGSASEVRCRSCAKPALGLEDTSDIWQDGSGKKFVGGTNTTLIALPSVFNYLTAELTAMNIKMMLEVK
InterPro domains: - IPR015712: DNA-directed RNA polymerase, subunit 2 (family) [21-1172]
- IPR037034: RNA polymerase Rpb2, domain 2 superfamily (homologous_superfamily) [193-364]
- IPR007121: RNA polymerase, beta subunit, conserved site (conserved_site) [899-911]
- IPR009674: DNA-directed RNA polymerase I subunit RPA2, domain 4 (domain) [568-636]
- IPR037033: DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily (homologous_superfamily) [686-1036]
- IPR007642: RNA polymerase Rpb2, domain 2 (domain) [191-377]
- IPR007644: RNA polymerase, beta subunit, protrusion (domain) [28-410]
- IPR007120: DNA-directed RNA polymerase, subunit 2, hybrid-binding domain (domain) [685-1050]
- IPR007641: RNA polymerase Rpb2, domain 7 (domain) [1052-1172]
- IPR007645: RNA polymerase Rpb2, domain 3 (domain) [462-527]
- IPR014724: RNA polymerase Rpb2, OB-fold (homologous_superfamily) [716-895]
Interaction partners: - DNA-directed RNA polymerases I, II, and III subunit RPABC2
- DNA-directed RNA polymerases I, II, and III subunit RPABC5
- DNA-directed RNA polymerase I subunit rpa1
- DNA-directed RNA polymerase I subunit rpa43
- DNA-directed RNA polymerase I subunit RPA12
- DNA-directed RNA polymerase I subunit rpa49
- DNA-directed RNA polymerases I, II, and III subunit RPABC1
- DNA-directed RNA polymerases I and III subunit RPAC1
- DNA-directed RNA polymerases I, II, and III subunit RPABC4
- DNA-directed RNA polymerases I, II, and III subunit RPABC3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0010467 (gene expression), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0016070 (RNA metabolic process), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006351 (DNA-templated transcription), GO:0006353 (DNA-templated transcription termination), GO:0006383 (transcription by RNA polymerase III)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000785 (chromatin), GO:0005829 (cytosol), GO:0140535 (intracellular protein-containing complex), GO:0098687 (chromosomal region), GO:1902494 (catalytic complex), GO:0140513 (nuclear protein-containing complex), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0030880 (RNA polymerase complex), GO:0000792 (heterochromatin), GO:1990234 (transferase complex), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043233 (organelle lumen), GO:0055029 (nuclear DNA-directed RNA polymerase complex), GO:0016591 (RNA polymerase II, holoenzyme), GO:0043227 (membrane-bounded organelle), GO:0000775 (chromosome, centromeric region), GO:0000428 (DNA-directed RNA polymerase complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0061695 (transferase complex, transferring phosphorus-containing groups), GO:0005665 (RNA polymerase II, core complex), GO:0005721 (pericentric heterochromatin), GO:0070013 (intracellular organelle lumen), GO:0005634 (nucleus), GO:0031981 (nuclear lumen), GO:0005694 (chromosome)