Protein: ATP-dependent DNA helicase 2 subunit KU80 (EC 3.6.4.12) (ATP-dependent DNA helicase 2 subunit 2) (ATP-dependent DNA helicase II 80 kDa subunit) (Q9FQ09)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU70, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA. Plays a role in maintaining telomere length. Acts as a negative regulator of telomerase. Binds to and recombines double-stranded T-DNA molecules.
Subcellular location: Nucleus {ECO:0000269|PubMed:12148535}. Cytoplasm {ECO:0000269|PubMed:12148535}. Note=Predominantly in the nucleus.
Sequence: MARNREGLVLVLDVGPAMRSVLPDVEKACSMLLQKKLIYNKYDEVGIVVFGTEETGNELAREIGGYENVTVLRNIRVVDELAAEHVKQLPRGTVAGDFLDALIVGMDMLIKMYGNAHKGKKRMCLITNAACPTKDPFEGTKDDQVSTIAMKMAAEGIKMESIVMRSNLSGDAHERVIEENDHLLTLFSSNAIAKTVNVDSPLSLLGSLKTRRVAPVTLFRGDLEINPTMKIKVWVYKKVAEERLPTLKMYSDKAPPTDKFAKHEVKVDYDYKVTAESTEVIAPEERIKGFRYGPQVIPISPDQIETLKFKTDKGMKLLGFTEASNILRHYYMKDVNIVVPDPSKEKSVLAVSAIAREMKETNKVAIVRCVWRNGQGNVVVGVLTPNVSERDDTPDSFYFNVLPFAEDVREFPFPSFNKLPSSWKPDEQQQAVADNLVKMLDLAPSAEEEVLKPDLTPNPVLQRFYEYLELKSKSTDATLPPMDGTFKRLMEQDPELSSNNKSIMDTFRGSFEVKENPKLKKASKRLLRDKPSGSDDEDNRMITYDAKENKIDIVGDANPIQDFEAMISRRDKTDWTEKAITQMKNLIMKLVENCTDEGDKALECVLALRKGCVLEQEPKQFNEFLNHLFKLCQERNLSHLLEHFMSKKITLIPKSEAADSDIVDENAGDFIVKQESMLES
InterPro domains: - IPR016194: SPOC-like, C-terminal domain superfamily (homologous_superfamily) [215-518]
- IPR006164: Ku70/Ku80, DNA-binding domain (domain) [226-430]
- IPR036494: Ku, C-terminal domain superfamily (homologous_superfamily) [527-676]
- IPR005161: Ku70/Ku80, N-terminal alpha/beta (domain) [8-161]
- IPR024193: Ku80 (family) [1-674]
- IPR005160: Ku70/Ku80 C-terminal arm (domain) [452-532]
- IPR036465: von Willebrand factor A-like domain superfamily (homologous_superfamily) [1-199]
- IPR014893: Ku, C-terminal (domain) [558-671]
Interaction partners: - ATP-dependent DNA helicase 2 subunit KU70
- DNA ligase 4
- DNA ligase 6
- DNA repair protein XRCC4
- Double-strand break repair protein MRE11
- Telomerase reverse transcriptase
- DNA ligase 1
- DNA ligase
- DNA polymerase lambda
- Chlorophyll a-b binding protein 3, chloroplastic
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003677 (DNA binding), GO:0003690 (double-stranded DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0009628 (response to abiotic stimulus), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0006807 (nitrogen compound metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0099402 (plant organ development), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0060322 (head development), GO:0046483 (heterocycle metabolic process), GO:0009314 (response to radiation), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0006970 (response to osmotic stress), GO:1901700 (response to oxygen-containing compound), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0071478 (cellular response to radiation), GO:0007420 (brain development), GO:0007399 (nervous system development), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0062197 (cellular response to chemical stress), GO:0006259 (DNA metabolic process), GO:0009651 (response to salt stress), GO:0007417 (central nervous system development), GO:0010212 (response to ionizing radiation), GO:1901701 (cellular response to oxygen-containing compound), GO:0006972 (hyperosmotic response), GO:0071479 (cellular response to ionizing radiation), GO:0010165 (response to X-ray), GO:0042538 (hyperosmotic salinity response), GO:0006281 (DNA repair), GO:0010332 (response to gamma radiation), GO:0048366 (leaf development), GO:0071481 (cellular response to X-ray), GO:0071475 (cellular hyperosmotic salinity response), GO:0006302 (double-strand break repair), GO:0006303 (double-strand break repair via nonhomologous end joining)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)