Protein: Cell division coordinator CpoB (P45955)
Organism: Escherichia coli (strain K12)
Function: Mediates coordination of peptidoglycan synthesis and outer membrane constriction during cell division. Promotes physical and functional coordination of the PBP1B-LpoB and Tol machines, and regulates PBP1B activity in response to Tol energy state.
Subcellular location: Periplasm {ECO:0000255|HAMAP-Rule:MF_02066, ECO:0000269|PubMed:8763928}. Note=Targeting to the Sec-translocase for transport across the inner membrane is SecB-dependent (PubMed:16352602). Localizes to the septum concurrent with PBP1B-LpoB and Tol at the onset of constriction (PubMed:25951518). Localization is dependent on divisome assembly and requires ongoing septal peptidoglycan synthesis (PubMed:25951518). {ECO:0000269|PubMed:16352602, ECO:0000269|PubMed:25951518}.
Sequence: MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQLERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLSSGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGNANTDYNAAIALVQDKSRQDDAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAADAMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM
InterPro domains: - IPR011990: Tetratricopeptide-like helical domain superfamily (homologous_superfamily) [139-263]
- IPR032519: YbgF, trimerisation domain (domain) [37-110]
- IPR019734: Tetratricopeptide repeat (repeat) [148-249]
- IPR034706: Cell division coordinator CpoB (family) [6-263]
- IPR014162: Cell division coordinator CpoB, C-terminal (domain) [144-260]
Interaction partners: - Peptidoglycan-associated lipoprotein
- Tol-Pal system protein TolB
- Tol-Pal system protein TolQ
- Penicillin-binding protein 1B
- Tol-Pal system protein TolR
- Acyl-CoA thioester hydrolase YbgC
- Tol-Pal system protein TolA
- Penicillin-binding protein activator LpoB
- Penicillin-binding protein 1A
- Probable peptidoglycan glycosyltransferase FtsW
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0042802 (identical protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044249 (cellular biosynthetic process), GO:0005975 (carbohydrate metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0044264 (cellular polysaccharide metabolic process), GO:0016051 (carbohydrate biosynthetic process), GO:0005976 (polysaccharide metabolic process), GO:0034637 (cellular carbohydrate biosynthetic process), GO:0000271 (polysaccharide biosynthetic process), GO:0033692 (cellular polysaccharide biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0031975 (envelope), GO:0071944 (cell periphery), GO:0042597 (periplasmic space), GO:0016020 (membrane), GO:0030312 (external encapsulating structure), GO:0009279 (cell outer membrane), GO:0019867 (outer membrane), GO:0030313 (cell envelope), GO:0030288 (outer membrane-bounded periplasmic space)