Protein: Peroxiredoxin TSA2 (Prx) (EC 1.11.1.24) (Cytoplasmic thiol peroxidase 2) (cTPx 2) (Thiol-specific antioxidant protein 2) (Thioredoxin peroxidase type Ib) (TPx type Ib) (Thioredoxin-dependent peroxiredoxin TSA2) (Q04120)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Can act alternatively as peroxidase and molecular chaperone. Oxidative stress and heat shock exposure cause a reversible shift of the protein structure from low MW species to high MW complexes, triggering a peroxidase-to-chaperone functional switch. The chaperone function of the protein enhances resistance to heat shock.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:10681558}.
Sequence: MVAEVQKQAPPFKKTAVVDGIFEEISLEKYKGKYVVLAFVPLAFSFVCPTEIVAFSDAAKKFEDQGAQVLFASTDSEYSLLAWTNLPRKDGGLGPVKVPLLADKNHSLSRDYGVLIEKEGIALRGLFIIDPKGIIRHITINDLSVGRNVNEALRLVEGFQWTDKNGTVLPCNWTPGAATIKPDVKDSKEYFKNANN
InterPro domains: - IPR024706: Peroxiredoxin, AhpC-type (family) [1-196]
- IPR036249: Thioredoxin-like superfamily (homologous_superfamily) [5-195]
- IPR013766: Thioredoxin domain (domain) [3-161]
- IPR019479: Peroxiredoxin, C-terminal (domain) [158-193]
- IPR000866: Alkyl hydroperoxide reductase subunit C/ Thiol specific antioxidant (domain) [5-138]
- IPR050217: Thiol-specific antioxidant peroxiredoxin (family) [3-194]
Interaction partners: - Sulfiredoxin
- Peroxiredoxin TSA1
- Thioredoxin-2
- Peroxiredoxin AHP1
- Catalase T
- Peroxiredoxin DOT5
- Glutathione peroxidase-like peroxiredoxin 1
- Thioredoxin reductase 1
- Glutathione peroxidase-like peroxiredoxin HYR1
- Superoxide dismutase, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016209 (antioxidant activity), GO:0030234 (enzyme regulator activity), GO:0044877 (protein-containing complex binding), GO:0004601 (peroxidase activity), GO:0005515 (protein binding), GO:0016491 (oxidoreductase activity), GO:0051082 (unfolded protein binding), GO:0016684 (oxidoreductase activity, acting on peroxide as acceptor), GO:0042802 (identical protein binding), GO:0019207 (kinase regulator activity), GO:0051920 (peroxiredoxin activity), GO:0043021 (ribonucleoprotein complex binding), GO:0043022 (ribosome binding), GO:0140824 (thioredoxin-dependent peroxiredoxin activity), GO:0008379 (thioredoxin peroxidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0042592 (homeostatic process), GO:0023052 (signaling), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0009628 (response to abiotic stimulus), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0065008 (regulation of biological quality), GO:0019725 (cellular homeostasis), GO:0044237 (cellular metabolic process), GO:0006457 (protein folding), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0048523 (negative regulation of cellular process), GO:0048585 (negative regulation of response to stimulus), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0009266 (response to temperature stimulus), GO:0044085 (cellular component biogenesis), GO:0060548 (negative regulation of cell death), GO:0035556 (intracellular signal transduction), GO:0045454 (cell redox homeostasis), GO:0006979 (response to oxidative stress), GO:0046483 (heterocycle metabolic process), GO:0080134 (regulation of response to stress), GO:0080135 (regulation of cellular response to stress), GO:1901700 (response to oxygen-containing compound), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0061077 (chaperone-mediated protein folding), GO:0031647 (regulation of protein stability), GO:0010948 (negative regulation of cell cycle process), GO:0043170 (macromolecule metabolic process), GO:0009408 (response to heat), GO:0009991 (response to extracellular stimulus), GO:0051726 (regulation of cell cycle), GO:1902883 (negative regulation of response to oxidative stress), GO:1901360 (organic cyclic compound metabolic process), GO:0010941 (regulation of cell death), GO:0006139 (nucleobase-containing compound metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0009889 (regulation of biosynthetic process), GO:0062012 (regulation of small molecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0045786 (negative regulation of cell cycle), GO:0016043 (cellular component organization), GO:0031668 (cellular response to extracellular stimulus), GO:0080090 (regulation of primary metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0010564 (regulation of cell cycle process), GO:0071496 (cellular response to external stimulus), GO:0034599 (cellular response to oxidative stress), GO:0043255 (regulation of carbohydrate biosynthetic process), GO:0000075 (cell cycle checkpoint signaling), GO:0006974 (cellular response to DNA damage stimulus), GO:0042770 (signal transduction in response to DNA damage), GO:0062197 (cellular response to chemical stress), GO:0010675 (regulation of cellular carbohydrate metabolic process), GO:1901032 (negative regulation of response to reactive oxygen species), GO:0010906 (regulation of glucose metabolic process), GO:0033194 (response to hydroperoxide), GO:0006109 (regulation of carbohydrate metabolic process), GO:1903202 (negative regulation of oxidative stress-induced cell death), GO:0042262 (DNA protection), GO:0022607 (cellular component assembly), GO:1900407 (regulation of cellular response to oxidative stress), GO:1903201 (regulation of oxidative stress-induced cell death), GO:0090304 (nucleic acid metabolic process), GO:1901987 (regulation of cell cycle phase transition), GO:1902882 (regulation of response to oxidative stress), GO:0034605 (cellular response to heat), GO:0006259 (DNA metabolic process), GO:0050821 (protein stabilization), GO:0043933 (protein-containing complex organization), GO:1901701 (cellular response to oxygen-containing compound), GO:1901988 (negative regulation of cell cycle phase transition), GO:0031570 (DNA integrity checkpoint signaling), GO:0065003 (protein-containing complex assembly), GO:0006111 (regulation of gluconeogenesis), GO:1901031 (regulation of response to reactive oxygen species), GO:1903206 (negative regulation of hydrogen peroxide-induced cell death), GO:1903205 (regulation of hydrogen peroxide-induced cell death), GO:0000077 (DNA damage checkpoint signaling), GO:0051258 (protein polymerization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:1990904 (ribonucleoprotein complex), GO:0005737 (cytoplasm), GO:0005844 (polysome)