Protein: Translational repressor ifet-1 (Spindle orientation defective protein 2) (Q20898)
Organism: Caenorhabditis elegans
Function: Involved in translational repression of multiple mRNAs in the distal gonad. Recruited to the 3' untranslated region (UTR) of zif-1 by oma-1 and is required for translational repression of zif-1. May also be involved in translational repression of mei-1 through recruitment to the mei-1 3' UTR by oma-1. Required for oogenesis but not spermatogenesis, for P granule formation and for the localization of car-1 and cgh-1 to P granules. Required for normal spindle orientation in early embryos.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:19786575, ECO:0000269|PubMed:23264733}. Note=In one-cell embryos, expressed highly and diffusely throughout the cytoplasm and is associated with P granules. {ECO:0000269|PubMed:19786575, ECO:0000269|PubMed:23264733}.
Sequence: MTSHLDDEGLHPMVSTLLGGGTLPTVPFKAYTRERMMELRTTKASMTRPENLSEDFNGEDGKFSPLKWLEHRWEIEGIKNRPMSKKIDSLCAGADENTGLSPQRRAFSSGCKAPTDDKGRDGEYERLGGHGKNWRNGSTGGADKFASRGNDFKPSFQKGNQLERGTRGAEWKKDTTRGAKFAPRREERLTSLSGSEKLPEWADGPTTMDDMIELRGFDEPKKVKNKKNPKEKKEKEAVKAPEPVECVGSRPSSTGLKTSEPIDDPAIAYSSSGGGALPATDQELAALLGCLDIQKTSRKIDGDDMAWAHKSEETAGGTSRLSRFFAKKNKSPELDAMLSSVGGGNDENVANPMLARLFGHSGGDNNASSSGAGDIKGGMRLEDLEKGMESKEPSKVSPLQDPSQQAQLLQHLQKFAKQQAESGQHIHHHRQPTPPNGGPQHQQHLHHPMVHPGMQIIADPSLLASFAQNPVILNAYVENQLQEAVNAAIRANNGQQLPPQLHEQLRMASMRNKAFLQSQTLTFVSLQQQHQNIQQHQQQQQHHQHKGRTPAMIPASVQRQLQKSSSNADQKKEKTSQSPPESNQETSDAHNQSDAMNQLKKLHMQQNYANMVQAMNSGVGWARGNGVVNGQQQHPQQQLPPNVQMLMAQHQQAQMQHLKMMMSRAQQQHMLMAKIAQMQQQQAQMANMQERQGPSHNQQQHQPVVPSELSQVGPIQTPLEKLLASVGVQGSQFTGSGDRIPSSVRPMSLEDLEKQLTAVPK
InterPro domains: - IPR018862: Eukaryotic translation initiation factor 4E binding protein (family) [7-422]
Interaction partners: - ATP-dependent RNA helicase cgh-1
- Protein LSM14 homolog car-1
- Eukaryotic translation initiation factor 4E-3
- CCCH-type zinc finger protein oma-1
- Protein lin-41
- Eukaryotic translation initiation factor 4E-2
- CCR4-NOT transcription complex subunit let-711
- Cytoplasmic polyadenylation element-binding protein 3
- Eukaryotic translation initiation factor 4E-4
- Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0065007 (biological regulation), GO:0032501 (multicellular organismal process), GO:0019222 (regulation of metabolic process), GO:0008340 (determination of adult lifespan), GO:0009892 (negative regulation of metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:1990904 (ribonucleoprotein complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0099080 (supramolecular complex), GO:0035770 (ribonucleoprotein granule), GO:0036464 (cytoplasmic ribonucleoprotein granule), GO:0005844 (polysome), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0000932 (P-body), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005634 (nucleus)