Protein: DNA mismatch repair protein MLH1 (MutL protein homolog 1) (Post meiotic segregation protein 2) (P38920)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Required for DNA mismatch repair (MMR), correcting base-base mismatches and insertion-deletion loops (IDLs) resulting from DNA replication, DNA damage or from recombination events between non-identical sequences during meiosis. Component of different MutL heterodimers that form a ternary complex with the MutS heterodimers, which initially recognize the DNA mismatches. This complex is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Plays a major role in maintaining the genetic stability of simple sequence repeats, the repair of heteroduplex sites present in meiotic recombination intermediates, and the promotion of meiotic crossing-over.
Subcellular location: Nucleus {ECO:0000269|PubMed:14562095}.
Sequence: MSLRIKALDASVVNKIAAGEIIISPVNALKEMMENSIDANATMIDILVKEGGIKVLQITDNGSGINKADLPILCERFTTSKLQKFEDLSQIQTYGFRGEALASISHVARVTVTTKVKEDRCAWRVSYAEGKMLESPKPVAGKDGTTILVEDLFFNIPSRLRALRSHNDEYSKILDVVGRYAIHSKDIGFSCKKFGDSNYSLSVKPSYTVQDRIRTVFNKSVASNLITFHISKVEDLNLESVDGKVCNLNFISKKSISPIFFINNRLVTCDLLRRALNSVYSNYLPKGNRPFIYLGIVIDPAAVDVNVHPTKREVRFLSQDEIIEKIANQLHAELSAIDTSRTFKASSISTNKPESLIPFNDTIESDRNRKSLRQAQVVENSYTTANSQLRKAKRQENKLVRIDASQAKITSFLSSSQQFNFEGSSTKRQLSEPKVTNVSHSQEAEKLTLNESEQPRDANTINDNDLKDQPKKKQKLGDYKVPSIADDEKNALPISKDGYIRVPKERVNVNLTSIKKLREKVDDSIHRELTDIFANLNYVGVVDEERRLAAIQHDLKLFLIDYGSVCYELFYQIGLTDFANFGKINLQSTNVSDDIVLYNLLSEFDELNDDASKEKIISKIWDMSSMLNEYYSIELVNDGLDNDLKSVKLKSLPLLLKGYIPSLVKLPFFIYRLGKEVDWEDEQECLDGILREIALLYIPDMVPKVDTSDASLSEDEKAQFINRKEHISSLLEHVLFPCIKRRFLAPRHILKDVVEIANLPDLYKVFERC
InterPro domains: - IPR036890: Histidine kinase/HSP90-like ATPase superfamily (homologous_superfamily) [3-218]
- IPR032189: DNA mismatch repair protein Mlh1, C-terminal (domain) [510-769]
- IPR014762: DNA mismatch repair, conserved site (conserved_site) [95-101]
- IPR014721: Small ribosomal subunit protein uS5 domain 2-type fold, subgroup (homologous_superfamily) [218-347]
- IPR038973: DNA mismatch repair protein MutL/Mlh/Pms-like (family) [4-696]
- IPR020568: Ribosomal protein uS5 domain 2-type superfamily (homologous_superfamily) [195-334]
- IPR013507: DNA mismatch repair protein, S5 domain 2-like (domain) [213-335]
- IPR002099: DNA mismatch repair protein MutL/Mlh/PMS (family) [5-632]
Interaction partners: - DNA mismatch repair protein MSH3
- DNA mismatch repair protein MSH2
- DNA mismatch repair protein MSH6
- MutS protein homolog 5
- DNA mismatch repair protein PMS1
- DNA mismatch repair protein MLH3
- Exodeoxyribonuclease 1
- DNA mismatch repair protein MLH2
- ATP-dependent helicase SGS1
- MutS protein homolog 4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0016887 (ATP hydrolysis activity), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:0003677 (DNA binding), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0016462 (pyrophosphatase activity), GO:0003690 (double-stranded DNA binding), GO:0003697 (single-stranded DNA binding), GO:0017111 (ribonucleoside triphosphate phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0022414 (reproductive process), GO:0009987 (cellular process), GO:0000003 (reproduction), GO:0044237 (cellular metabolic process), GO:0051321 (meiotic cell cycle), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:0051716 (cellular response to stimulus), GO:0071840 (cellular component organization or biogenesis), GO:1903046 (meiotic cell cycle process), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0061982 (meiosis I cell cycle process), GO:1901360 (organic cyclic compound metabolic process), GO:0140013 (meiotic nuclear division), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0007131 (reciprocal meiotic recombination), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0006259 (DNA metabolic process), GO:0006996 (organelle organization), GO:0007127 (meiosis I), GO:0048285 (organelle fission), GO:0006310 (DNA recombination), GO:0006281 (DNA repair), GO:0000280 (nuclear division), GO:0035825 (homologous recombination), GO:0006298 (mismatch repair), GO:0140527 (reciprocal homologous recombination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0032300 (mismatch repair complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140513 (nuclear protein-containing complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)