Protein: Isoleucine--tRNA ligase, cytoplasmic (EC 6.1.1.5) (Isoleucyl-tRNA synthetase) (IRS) (IleRS) (Q8BU30)
Organism: Mus musculus (Mouse)
Function: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:P41252}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:P41252}.
Sequence: MVQQVPENISFPAEEEKILEFWSKHNCFQECLKQSKLRPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIAEYNKQCRAIVMRYSAEWKSTVTRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACGTPLSNFESNQNYKDVQDPSVFVTFPLEEDENTSLVAWTTTPWTLPSNLALCVNPEIQYVKIKDVARGKLFILTEARLSALYKQESDYEILERFPGASLKGKKYKPLFDYFIKCKENGAFTVLVDHYVKDEEGTGVVHQAPYFGADDHRVCMDFNIIQKDSVPVCPVDASGCFTEEVTHFVGQYVKDADKNIIRMLKEQGRLLAAGTFTHSYPFCWRSDTPLIYKSVPSWFVRVEPMVDQLLKNNDLCYWVPEFVREKRFGNWLKEARDWAISRNRYWGTPIPLWVSEDLEEVVCIGSVAELEELSGTKISDLHRESIDHLTIPSRCGKAPLRRVSEVFDCWFESGSMPYAQVHYPFESKREFEDAFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFKNVIVNGLILASDGQKMSKRKKNYPDPVSIIDKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVFRLHKEEEVKFLYNEHTVRESPNITDRWVLSFMQSLLGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGESGVEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKLLIDPASLRDKDTLSIHYLMLPRVREELIDKKTENAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALEDIRSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMMAIKRLSNEELERFQKSGSIVVEGHELHEEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYNAKSEGRYLNNVIESHTDFIFATIKAPLKPYPVPTSDNILIQEQTQLKGSELEITLTKGSCVPGPACAYVNLNICANGTEQGGVLLLENPKGDNQLNLVKLKTVVTSVFGVKNAKLSVFHGETEIQNQTDLLSLSGRTLCVTAGASPSPISSPSTLLCQYLNLQLLNAEPQECLTGTVGTLLLENPLGQNGLTHQGLVHEAAKVFGLRSRRLRLFLNETQTQEITEDIPMKTLNMKTVYVSVLPTTADG
InterPro domains: - IPR009080: Aminoacyl-tRNA synthetase, class Ia, anticodon-binding (homologous_superfamily) [655-910]
- IPR002300: Aminoacyl-tRNA synthetase, class Ia (domain) [17-638]
- IPR033709: Isoleucyl tRNA synthetase type 2, anticodon-binding domain (domain) [640-833]
- IPR009008: Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain (homologous_superfamily) [199-397]
- IPR023586: Isoleucine-tRNA ligase, type 2 (family) [1-1070]
- IPR057033: Isoleucine--tRNA ligase, cytoplasmic, ubiquitin-like domain (domain) [1077-1259]
- IPR013155: Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding (domain) [693-849]
- IPR001412: Aminoacyl-tRNA synthetase, class I, conserved site (conserved_site) [48-59]
- IPR002301: Isoleucine-tRNA ligase (family) [5-861]
- IPR014729: Rossmann-like alpha/beta/alpha sandwich fold (homologous_superfamily) [2-639]
Interaction partners: - Leucine--tRNA ligase, cytoplasmic
- Methionine--tRNA ligase, cytoplasmic
- Bifunctional glutamate/proline--tRNA ligase
- Lysine--tRNA ligase
- Glutamine--tRNA ligase
- Arginine--tRNA ligase, cytoplasmic
- Methionine--tRNA ligase, mitochondrial
- Probable leucine--tRNA ligase, mitochondrial
- Aspartate--tRNA ligase, cytoplasmic
- Tyrosine--tRNA ligase, cytoplasmic
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0140098 (catalytic activity, acting on RNA), GO:0016875 (ligase activity, forming carbon-oxygen bonds), GO:0004812 (aminoacyl-tRNA ligase activity), GO:0140101 (catalytic activity, acting on a tRNA)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006082 (organic acid metabolic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006412 (translation), GO:0009059 (macromolecule biosynthetic process), GO:0006518 (peptide metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0043038 (amino acid activation), GO:0010467 (gene expression), GO:0019752 (carboxylic acid metabolic process), GO:0016070 (RNA metabolic process), GO:0043043 (peptide biosynthetic process), GO:0006418 (tRNA aminoacylation for protein translation), GO:0043039 (tRNA aminoacylation), GO:0034660 (ncRNA metabolic process), GO:0006399 (tRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)