Protein: Cell cycle protein kinase DBF2 (Q59KM8)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Ser/Thr-protein kinase involved in the mitotic exit network (MEN) and required after the metaphase to anaphase cell cycle transition. Required for proper nuclear segregation, mitotic spindle organization, actomyosin ring contraction, primary septum assembly, and normal hyphal morphogenesis
Subcellular location: Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm, cytoskeleton, spindle. Nucleus. Bud neck. Note=In small unbudded G1 cells, localizes to the spindle pole body (SPB). At the G1/S transition, remains associated with the SPB but also starts to accumulate in a linear structure in the nuclei of the cells. During mitosis, a very faint signal along the mother-bud axis is observed, suggesting association with the mitotic spindle during this part of the cell cycle. Finally, localizes to the bud neck at the end of mitosis and cytokinesis
Sequence: MTNFFNRSPKHQSHHYQPHQQDVTDISYSMENVSISSNAMMDIDTSYRSSKPTYNNPQQQQQQQQQQQAQNNLFNKENITPLNSPTKSILHNSPQQAKSSTSPQHLYNKLVNANYNGNSPQPGIQQQQNNRALQNNINQLQPPLNKRYKLTEAEFYAKANSARTKRLTSIAQLYFLDYYCDMFDYVINRRERTAIVEKNLLTDPMYKNDITKQQFEWKNYIGRERALLRKRRLKPKHKDFEMITQIGQGGYGQVFLSRKRDTREICALKILNKKLLIKLDETRHVLTERDILTNTRSDWLVKLLYAFQDQEKVFLAMEFVPGGDFRTLLNNTGYLIPPHARFYISEMFAAVNSLHELGFTHRDLKPENFLIDSKGHIKLTDFGLAAGTVCNDRIESMKIKLQNLQNLNDFNDDSNNDNHHYQVPSSLIYERQKIFKQSQQQQQQQNSNNTTANSIVGSPDYMALEVLEGKNYNYTIDYWSLGCMLFEALCGYPPFSGSKQDETYYNLKHWKTALRRPQTKDGRYVFSDRTWNLIIKLIASPNNRLQNFKQVQQQSYFSDIKDWGNLRQKTPPFTPQLDNEEDAGYFDDFEDDEMMMKYKDVFARQEQNEQLLEKSNTTTTTTTTTTTKNGKRFSPGSKFNDNFIGFTFKHKSNPNNKFTNGSGNTGRYGNGNGNNNNNGEINLLNMVENGNGIGNGNSRSSRLNPLATLY
InterPro domains: - IPR008271: Serine/threonine-protein kinase, active site (active_site) [359-371]
- IPR050236: Serine/threonine-protein kinases, AGC (family) [68-599]
- IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [219-578]
- IPR017441: Protein kinase, ATP binding site (binding_site) [246-269]
- IPR000961: AGC-kinase, C-terminal (domain) [558-658]
- IPR000719: Protein kinase domain (domain) [240-557]
Interaction partners: - Mob1p
- Tyrosine-protein phosphatase CDC14
- Serine/threonine protein kinase
- Ras family GTPase
- Serine/threonine-protein kinase
- Serine/threonine protein kinase
- Ras GTPase-activating-like protein IQG1
- Protein phosphatase PP2A regulatory subunit B
- Uncharacterized protein
- G2/mitotic-specific cyclin CLB2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050794 (regulation of cellular process), GO:0051128 (regulation of cellular component organization), GO:1903338 (regulation of cell wall organization or biogenesis), GO:0060237 (regulation of fungal-type cell wall organization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)