Protein: RecBCD enzyme subunit RecD (EC 5.6.2.3) (DNA 5'-3' helicase subunit RecB) (Exonuclease V subunit RecD) (ExoV subunit RecD) (Helicase/nuclease RecBCD subunit RecD) (P9WHJ1)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD.
Sequence: MKLTDVDFAVEASGMVRAFNQAGVLDVSDVHVAQRLCALAGESDERVALAVAVAVRALRAGSVCVDLLSIARVAGHDDLPWPDPADWLAAVRASPLLADPPVLHLYDDRLLYLDRYWREEEQVCADLLALLTSRRPAGVPDLRRLFPTGFDEQRRAAEIALSQGVTVLTGGPGTGKTTTVARLLALVAEQAELAGEPRPRIALAAPTGKAAARLAEAVRREMAKLDATDRARLGDLHAVTLHRLLGAKPGARFRQDRQNRLPHNVIVVDETSMVSLTLMARLAEAVRPGARLILVGDADQLASVEAGAVLADLVDGFSVRDDALVAQLRTSHRFGKVIGTLAEAIRAGDGDAVLGLLRSGEERIEFVDDEDPAPRLRAVLVPHALRLREAALLGASDVALATLDEHRLLCAHRDGPTGVLHWNRRVQAWLAEETGQPPWTPWYAGRPLLVTANDYGLRVYNGDTGVVLAGPTGLRAVISGASGPLDVATGRLGDVETMHAMTIHKSQGSQVDEVTVLMPQEDSRLLTRELLYTAVTRAKRKVRVVGSEASVRAAIARRAVRASGLRMRLQSTGCG
InterPro domains: - IPR050534: Coronaviruses polyprotein 1ab (family) [129-547]
- IPR041851: RecBCD enzyme subunit RecD, N-terminal domain superfamily (homologous_superfamily) [13-114]
- IPR027785: UvrD-like helicase C-terminal domain (domain) [499-545]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [20-567]
- IPR006344: RecBCD enzyme subunit RecD (family) [17-569]
- IPR049550: RecBCD enzyme subunit RecD, N-terminal domain (domain) [21-100]
Interaction partners: - RecBCD enzyme subunit RecC
- RecBCD enzyme subunit RecB
- Transcriptional repressor NrdR
- Cobalamin biosynthesis protein CobN
- DNA polymerase I
- DNA polymerase IV 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0036094 (small molecule binding), GO:0004386 (helicase activity), GO:1901363 (heterocyclic compound binding), GO:0008094 (ATP-dependent activity, acting on DNA), GO:0016787 (hydrolase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0003678 (DNA helicase activity), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0032553 (ribonucleotide binding), GO:0140097 (catalytic activity, acting on DNA), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0017076 (purine nucleotide binding), GO:0017116 (single-stranded DNA helicase activity), GO:0004518 (nuclease activity), GO:0004536 (deoxyribonuclease activity), GO:0032555 (purine ribonucleotide binding), GO:0005524 (ATP binding), GO:0004527 (exonuclease activity), GO:0032559 (adenyl ribonucleotide binding), GO:0004529 (DNA exonuclease activity), GO:0030554 (adenyl nucleotide binding), GO:0016895 (DNA exonuclease activity, producing 5'-phosphomonoesters), GO:0016796 (exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0010467 (gene expression), GO:0018130 (heterocycle biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0016070 (RNA metabolic process), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006351 (DNA-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0005886 (plasma membrane), GO:0009274 (peptidoglycan-based cell wall)