Protein: Probable tRNA(Ile)-lysidine synthase (EC 6.3.4.19) (tRNA(Ile)-2-lysyl-cytidine synthase) (tRNA(Ile)-lysidine synthetase) (Q10441)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine (By similarity).
Subcellular location: Cytoplasm {ECO:0000305}.
Sequence: MDTIKLKEFYNSLVSIRRRVGHRRLGIAVSGGVDSMLLSWFMKESQIMFGWPNQFIAFVVDHRIRKNSTEEALQTIWNLNRMLIPNVYLNINWGNDDVHSLTNLETIAREHRYQVLTRACITHNIRHICTAHHANDQAETIFMRLLRRKPGTWGGLCAMKPVSQIPESDSICGASNIELLRPLLPYYKNQILNTAKQHGIAWEEDPTNADINLTPRNAIRRFLNQHAALTVEATKLATAFQSLQVNIDNKVDEILKDNIVSYHQPSGTLSLCFSKNELKKHSNLTKEELLLRCLTLTTSCRQIKRSSVVSLCNSVFDGKKLTIAKCLLTSSSIKDDTKLQLQISRQPFSKAELKKKTITINPDRYVLWDHRFWIKYSCKNTQTTLILRPLLSKDLNSLKGFLSKEEFLKFCIQVPGHIRFTIPVLSEENDKLVGIPTFGFNFRSDIISNCLHKFKL
InterPro domains: - IPR014729: Rossmann-like alpha/beta/alpha sandwich fold (homologous_superfamily) [8-228]
- IPR011063: tRNA(Ile)-lysidine/2-thiocytidine synthase, N-terminal (domain) [26-221]
- IPR012795: tRNA(Ile)-lysidine synthase, N-terminal (domain) [24-223]
- IPR012094: tRNA(Ile)-lysidine synthase (family) [9-436]
Interaction partners: - Cytoplasmic tRNA 2-thiolation protein 2
- RCC1 repeat-containing protein C10F6.04
- tRNA modification GTPase mss1
- Probable 3-mercaptopyruvate sulfurtransferase
- Probable tRNA-splicing endonuclease subunit sen54
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0016740 (transferase activity), GO:0003676 (nucleic acid binding), GO:0140098 (catalytic activity, acting on RNA), GO:0016782 (transferase activity, transferring sulphur-containing groups), GO:0003723 (RNA binding), GO:0016783 (sulfurtransferase activity), GO:0140101 (catalytic activity, acting on a tRNA), GO:0000049 (tRNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0043933 (protein-containing complex organization), GO:0010467 (gene expression), GO:0022607 (cellular component assembly), GO:0016070 (RNA metabolic process), GO:0022618 (ribonucleoprotein complex assembly), GO:0065003 (protein-containing complex assembly), GO:0006396 (RNA processing), GO:0071826 (ribonucleoprotein complex subunit organization), GO:0034470 (ncRNA processing), GO:0043248 (proteasome assembly), GO:0006364 (rRNA processing), GO:0034660 (ncRNA metabolic process), GO:0016072 (rRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)