Protein: Deoxyhypusine synthase (EC 2.5.1.46) (Q9FI94)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Also able to produce homospermidine from putrescine (By similarity).
Sequence: MEDDRVFSSVHSTVFKESESLEGKCDKIEGYDFNQGVDYPKLMRSMLTTGFQASNLGEAIDVVNQMLDWRLADETTVAEDCSEEEKNPSFRESVKCKIFLGFTSNLVSSGVRDTIRYLVQHHMVDVIVTTTGGVEEDLIKCLAPTFKGDFSLPGAYLRSKGLNRIGNLLVPNDNYCKFEDWIIPIFDEMLKEQKEENVLWTPSKLLARLGKEINNESSYLYWAYKMNIPVFCPGLTDGSLGDMLYFHSFRTSGLIIDVVQDIRAMNGEAVHANPKKTGMIILGGGLPKHHICNANMMRNGADYAVFINTGQEFDGSDSGARPDEAVSWGKIRGSAKTVKVYCDATIAFPLLVAETFATKRDQTCESKT
InterPro domains: - IPR002773: Deoxyhypusine synthase (family) [6-365]
- IPR029035: DHS-like NAD/FAD-binding domain superfamily (homologous_superfamily) [8-362]
- IPR036982: Deoxyhypusine synthase superfamily (homologous_superfamily) [9-364]
Interaction partners: - Eukaryotic translation initiation factor 5A-1
- Eukaryotic translation initiation factor 5A-2
- Deoxyhypusine hydroxylase
- Eukaryotic translation initiation factor 5A-3
- Elongation factor P
- Elongation factor P
- ARM repeat superfamily protein
- Deoxyhypusine protein
- At5g44730
- Nascent polypeptide-associated complex subunit beta
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016765 (transferase activity, transferring alkyl or aryl (other than methyl) groups)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0042592 (homeostatic process), GO:0048878 (chemical homeostasis), GO:0009058 (biosynthetic process), GO:0051240 (positive regulation of multicellular organismal process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0051239 (regulation of multicellular organismal process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0010632 (regulation of epithelial cell migration), GO:0048580 (regulation of post-embryonic development), GO:2000026 (regulation of multicellular organismal development), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0048582 (positive regulation of post-embryonic development), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0043170 (macromolecule metabolic process), GO:0036211 (protein modification process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006412 (translation), GO:0009059 (macromolecule biosynthetic process), GO:0043412 (macromolecule modification), GO:0006518 (peptide metabolic process), GO:0009308 (amine metabolic process), GO:0051604 (protein maturation), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0010467 (gene expression), GO:0043043 (peptide biosynthetic process), GO:0006576 (biogenic amine metabolic process), GO:0018193 (peptidyl-amino acid modification), GO:0006595 (polyamine metabolic process), GO:0018205 (peptidyl-lysine modification), GO:0008216 (spermidine metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)