Protein: Protein artemis (mArt) (EC 3.1.-.-) (DNA cross-link repair 1C protein) (SNM1-like protein) (Q8K4J0)
Organism: Mus musculus (Mouse)
Function: Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein likely exhibits single-strand specific 5'-3' exonuclease activity in isolation, and may acquire endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity may be required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.
Subcellular location: Nucleus {ECO:0000250}.
Sequence: MSSFQGQMAEYPTISIDRFDRENLKARAYFLSHCHKDHMKGLRAPSLKRRLECSLKVFLYCSPVTKELLLTSPKYRFWENRIITIEIETPTQISLVDEASGEKEEVVVTLLPAGHCPGSVMFLFQGSNGTVLYTGDFRLAKGEASRMELLHSGGRVKDIQSVYLDTTFCDPRFYQIPSREQCLRGILELVRSWVTRSPHHVVWLNCKAAYGYEYLFTNLSEELGVQVHVDKLDMFKNMPDILHHLTTDRNTQIHACRHPKAEECFQWNKLPCGITSQNKTALHTISIKPSTMWFGERTRKTNVIVRTGESSYRACFSFHSSFSEIKDFLSYICPVNVYPNVIPVGLTVDKVMDVLKPLCRSPQSVEPKYKPLGKLKRARTIHLDSEEDDDLFDDPLPTPLRHKVPYQLTLQPELFSMKALPLDQPELRQSPGGCKAESVWSPSLANFIDCEESNSDSGEELETPPPSLQGGLGPSTLVQQNADPDVDIPQWEVFFKRRDEITGECLEHLPSSIETGGSQSPKLCSDSPKLCSDSPKLCSDSDGDSTHISSQNSSQSTHITDQGSQGWDSQCDTVLLSSQEKSGGDSTSLNKGAYKPKLKESISASQIEQDALCPQDTHCDLKSRAEVNGAPCLVELDTLSGRKSPPEKTLLSSTRADSQSSSDFEIPSTPEAELPTPEHLQCLYRKLATGQSIVVEKRKCSLLDS
InterPro domains: - IPR011084: DNA repair metallo-beta-lactamase (domain) [240-343]
- IPR036866: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like (homologous_superfamily) [11-341]
Interaction partners: - DNA-dependent protein kinase catalytic subunit
- X-ray repair cross-complementing protein 5
- X-ray repair cross-complementing protein 6
- DNA ligase 4
- Non-homologous end-joining factor 1
- DNA repair protein XRCC4
- DNA repair protein XRCC1
- DNA excision repair protein ERCC-5
- Serine-protein kinase ATM
- Breast cancer type 2 susceptibility protein homolog
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0005515 (protein binding), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0140097 (catalytic activity, acting on DNA), GO:0004518 (nuclease activity), GO:0004536 (deoxyribonuclease activity), GO:0004527 (exonuclease activity), GO:0004520 (DNA endonuclease activity), GO:0004519 (endonuclease activity), GO:0008409 (5'-3' exonuclease activity), GO:0000014 (single-stranded DNA endodeoxyribonuclease activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0006259 (DNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0006281 (DNA repair), GO:0006302 (double-strand break repair), GO:0006303 (double-strand break repair via nonhomologous end joining)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098687 (chromosomal region), GO:0043229 (intracellular organelle), GO:0000781 (chromosome, telomeric region), GO:0043228 (non-membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome)