Protein: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (AtGFAT) (AtGFAT1) (Glutamine:fructose-6-phosphate amidotransferase 1) (EC 2.6.1.16) (D-fructose-6-phosphate amidotransferase GFAT1) (Hexosephosphate aminotransferase GFAT1) (Q9LIP9)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Controls the flux of glucose into the hexosamine biosynthetic pathway (HBP) leading to glucosamine (GlcN) content homeostasis. Involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Required during pollen maturation and pollen tube formation by triggering polar deposition of pectin and callose in the pollen cell wall. Promotes tolerance to tunicamycin (Tm), an inhibitor of proteins N-glycosylation in endoplasmic reticulum (ER).
Sequence: MCGIFAYLNFHANKERRYILDVLFNGLRRLEYRGYDSAGIAIDNSSPSSSPLVFRQAGNIESLVNSVNEEITNTDLNLDEVFYFHAGIAHTRWATHGEPAPRNSHPQSSGPGDDFLVVHNGVITNYEVLKETLVRHGFTFESDTDTEVIPKLAKFVFDKANEEGGQTVTFCEVVFEVMRHLEGAYALIFKSWHYPNELIACKLGSPLLLGVKELDQGESNSHVFQDAHFLSKNDHPKEFFLSSDPHALVEHTKKVLVIEDGEVVNLKDGGVSILKFENERGRCNGLSRPASVERALSVLEMEVEQISKGKYDHYMQKEIHEQPESLTTTMRGRLIRGGSRKTKTVLLGGLKDHLKTIRRSRRIVFIGCGTSYNAALASRPILEELSGIPVSMEIASDLWDRQGPIYREDTAVFVSQSGETADTLLALDYARENGALCVGITNTVGSSIARKTHCGVHINAGAEIGVASTKAYTSQIVVMVMLALAIGSDTISSQKRREAIIDGLLDLPYKVKEVLKLDDEMKDLAQLLIDEQSLLVFGRGYNYATALEGALKVKEVALMHSEGILAGEMKHGPLALVDENLPIAVIATRDACFSKQQSVIQQLHARKGRLIVMCSKGDAASVSSSGSCRAIEVPQVEDCLQPVINIVPLQLLAYHLTVLRGHNVDQPRNLAKSVTTQ
InterPro domains: - IPR035490: GlmS/FrlB, SIS domain 2 (domain) [519-675]
- IPR029055: Nucleophile aminohydrolases, N-terminal (homologous_superfamily) [2-285]
- IPR047084: Glucosamine-fructose-6-phosphate aminotransferase, isomerising, N-terminal domain (domain) [2-264]
- IPR001347: SIS domain (domain) [353-667]
- IPR035466: GlmS/AgaS, SIS domain 1 (domain) [362-487]
- IPR046348: SIS domain superfamily (homologous_superfamily) [308-676]
- IPR017932: Glutamine amidotransferase type 2 domain (domain) [2-269]
- IPR005855: Glucosamine-fructose-6-phosphate aminotransferase, isomerising (family) [237-675]
Interaction partners: - Glucosamine 6-phosphate N-acetyltransferase
- Glucose-6-phosphate isomerase, cytosolic
- Glucose-6-phosphate isomerase 1, chloroplastic
- Phosphoacetylglucosamine mutase
- Glutamine synthetase, chloroplastic/mitochondrial
- Glutamine synthetase cytosolic isozyme 1-3
- Glutamine synthetase cytosolic isozyme 1-2
- Glutamine synthetase cytosolic isozyme 1-1
- Glutamine synthetase cytosolic isozyme 1-4
- Glutamine synthetase cytosolic isozyme 1-5
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016769 (transferase activity, transferring nitrogenous groups), GO:0008483 (transaminase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0044281 (small molecule metabolic process), GO:0009719 (response to endogenous stimulus), GO:0006807 (nitrogen compound metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:1901135 (carbohydrate derivative metabolic process), GO:0006793 (phosphorus metabolic process), GO:0006002 (fructose 6-phosphate metabolic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006047 (UDP-N-acetylglucosamine metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0006040 (amino sugar metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0009225 (nucleotide-sugar metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0046349 (amino sugar biosynthetic process), GO:0009226 (nucleotide-sugar biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)