Protein: Protein argonaute (A0A1M5A5Z8)
Organism: Marinitoga hydrogenitolerans (strain DSM 16785 / JCM 12826 / AT1271)
Function: A highly versatile argonaute that uses 5'-phospho- and 5'- OH- guide RNA (gRNA) or DNA (gDNA) to cleave target RNA or ssDNA (tDNA) in all possible combinations; has no detectable activity in the absence of guide. Uses short guide sequences (18-21 nucleotides (nt) on average) to bind complementary target nucleic acids resulting in target cleavage in a site-specific manner. Using 5'-phospho-gRNA or 5'-OH-gRNA the cleavage site is 10 nt downstream of the target residue base-paired with the 5'-end of the gRNA, using 5'-phospho-gDNA the cleavage site is 11 nucleotides (nt) downstream, while with 5'-OH-gDNA the cleavage site is 9 nt downstream
Sequence: MYLNLYEIKIPYRVKRLYYFNKENDPKEFARNLSRVNNIRFNDSKDLVWLEIPDIDFKITPQQAEKYKIEKNEIIGEKEDSDLFVKTIYRYIKKKFIDNNFYYKRGNNYISINDKFPLDSNTNVNAHLTYKIKLYKINERYYISVLPKFTFLSDKPALESPIKSTYLFNIKSGKTFPYISGLNGVLKIDLGENGIKEVLFPENYYFNFTSKEAEKFGFSKEIHNIYKEKIFSGYKKIKQSLYFLEDIININNYNLTMDKKIYVNIEYEFKKGISRNIKDVFKYSFYKNDQKIKIAFFFSSKKQIYEIQRSLKMLFQNKNSIFYQTIYEMGFSKVIFLREPKTNSSAFMYNPETFEISNKDFFENLEGNIMAIIILDKFLGNIDSLIQKFPENLILQPILKEKLEKIQPYIIKSYVYKMGNFIPECQPYVIRNLKDKNKTLYIGIDLSHDNYLKKSNLAISAVNNFGDIIYLNKYKNLELNEKMNLDIVEKEYIQILNEYYERNKNYPENIIVLRDGRYLEDIEIIKNILNIENIKYSLIEVNKSVNINSCEDLKEWIIKLSDNNFIYYPKTYFNQKGVEIKIIENNTDYNNEKILEQVYSLTRVVHPTPYVNYRLPYPLQVVNKVALTELEWKLYIPYMK
InterPro domains: - IPR054434: Argonaute, middle domain, bacteria (domain) [262-430]
- IPR054387: Argonaute, N-terminal domain, bacteria (domain) [13-75]
- IPR003165: Piwi domain (domain) [369-633]
- IPR012337: Ribonuclease H-like superfamily (homologous_superfamily) [410-626]
- IPR036397: Ribonuclease H superfamily (homologous_superfamily) [433-604]
Interaction partners: - CRISPR-associated endonuclease Cas1
- TOTE conflict system primase domain-containing protein
- CRISPR-associated endoribonuclease Cas2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0036260 (RNA capping), GO:0006370 (7-methylguanosine mRNA capping), GO:0009452 (7-methylguanosine RNA capping)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0018995 (host cellular component), GO:0043657 (host cell), GO:0033643 (host cell part), GO:0033646 (host intracellular part), GO:0043656 (host intracellular region), GO:0033647 (host intracellular organelle), GO:0033648 (host intracellular membrane-bounded organelle), GO:0042025 (host cell nucleus)