Protein: Enhanced filamentous growth protein 1 (Q59X67)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Transcriptional regulator of the switch between 2 heritable states, the white and opaque states. These 2 cell types differ in many characteristics, including cell structure, mating competence, and virulence. Each state is heritable for many generations, and switching between states occurs stochastically, at low frequency. Antagonizes the action of WOR1, WOR2 and CZF1, and promotes the white state. In white cells, EFG1 represses WOR1 indirectly through WOR2 to maintain white cell identity. Binds target gene promoters at the EFG1 recognition sequence (EGRbox) TATGCATA. Acts as a major regulator of cell wall dynamics and plays a role in interactions with extracellular matrices. Required for TOR1-dependent cellular aggregation and adhesin expression. Required for both normoxic and hypoxic biofilm formation. Hypoxic biofilm formation is a major cause of perseverance and antifungal resistance during infections. Contributes to virulence by regulating hyphal formation and the factors that enable C.albicans to invade and injure endothelial cells. Required for the formation of thick-walled big resting spores called chlamydospores, which survive in unfavorable conditions. Mediates the expression of virulence factors SAP4, SAP5and SAP6 during infection. Involved in drug resistance by regulating the expression of ERG3
Subcellular location: Nucleus
Sequence: MSTYSIPYYNQMNGNYNNGMPQQTTAANQQAFPQQQQPTTTGNASQQQQQAAATAAAAAVQQPYNYMFYQQQGQPGQQTGQTAGQQQQQQQQQQQYDYNTYNRYQYPAATSQGNYYQQTIPNQLSQPQPQHYNGSNRNYTSAPSGAPIPSNSTSGPSQQPPLPGQQAVPIPPHVSTMQQPTPVQDTLNASSTSTVGQFQPPGIRPRVTTTMWEDEKTLCYQVDANNVSVVRRADNNMINGTKLLNVAQMTRGRRDGILKSEKVRHVVKIGSMHLKGVWIPFERALAMAQREQIVDMLYPLFVRDIKRVIQTGVTPNAAAATAAAAATATSASAPPPPPPPVAAATTTAATAISKSSSGNGNSISATSGGSNVSGASGAGSTTSPVNTKAATTAGTPQGNYYQTYNQQQYPQQYGQYNAPGKNQNTPASQPGSTTNDQYLQQQQQMYGYQSNYYQGGAANSSYYPNYYQQQQPNYASSYPYQQQQQKQQQQQPNQQQQSDQQQTSTPSGGAGTRSVHQSPQVQSLTQGSVHPSPQQHQANQSASTVAKEEK
InterPro domains: - IPR036887: HTH APSES-type DNA-binding domain superfamily (homologous_superfamily) [206-333]
- IPR029790: EFG1/Phd1/StuA family (family) [1-543]
- IPR018004: KilA/APSES-type HTH, DNA-binding (domain) [225-311]
- IPR003163: Transcription regulator HTH, APSES-type DNA-binding domain (domain) [206-312]
Interaction partners: - cAMP-dependent protein kinase
- cAMP-dependent protein kinase
- Transcriptional regulator of filamentous growth FLO8
- Transcription activator TEC1
- Zinc cluster transcription factor CZF1
- Transcriptional regulator NRG1
- Transcriptional regulator EFH1
- Transcriptional repressor TUP1
- Transcriptional repressor TUP1
- Biofilm and cell wall regulator 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0140110 (transcription regulator activity), GO:0003700 (DNA-binding transcription factor activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0040007 (growth), GO:0048522 (positive regulation of cellular process), GO:0019222 (regulation of metabolic process), GO:0071554 (cell wall organization or biogenesis), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0030447 (filamentous growth), GO:0071840 (cellular component organization or biogenesis), GO:0045927 (positive regulation of growth), GO:0040008 (regulation of growth), GO:0044182 (filamentous growth of a population of unicellular organisms), GO:0071852 (fungal-type cell wall organization or biogenesis), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0010570 (regulation of filamentous growth), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0016043 (cellular component organization), GO:0080090 (regulation of primary metabolic process), GO:0071555 (cell wall organization), GO:0009891 (positive regulation of biosynthetic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0031505 (fungal-type cell wall organization), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:1900428 (regulation of filamentous growth of a population of unicellular organisms), GO:0051252 (regulation of RNA metabolic process), GO:0045229 (external encapsulating structure organization), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0010468 (regulation of gene expression), GO:1902680 (positive regulation of RNA biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0045944 (positive regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)