Protein: Protein phosphatase 1 regulatory subunit 12B (Myosin phosphatase-targeting subunit 2) (Myosin phosphatase target subunit 2) (Q8BG95)
Organism: Mus musculus (Mouse)
Function: Regulates myosin phosphatase activity. Augments Ca(2+) sensitivity of the contractile apparatus (By similarity).
Subcellular location: Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:O60237}. Cytoplasm, cytoskeleton, stress fiber {ECO:0000250|UniProtKB:O60237}. Note=Along actomyosin filaments. {ECO:0000250|UniProtKB:O60237}.
Sequence: MAELEHLGGKRAESARARRAEQLRRWRGSLTEQEPAERQGAGRQLQTRRGSPRVRFEDGAVFLAACSSGDTDEVKKLLARGADINTVNVDGLTALHQACIDENLDMVKFLVENRANVNQQDNEGWTPLHAAASCGYLNIAEYFISHGASVGIVNSEGEVPSDLAEEPAMKDLLLEQVKKQGVDLEQSRKEEEQQMLQDARQWLNSGRIEDVRQARSGATALHVAAAKGYSEVLRLLIQAGYELNVQDHDGWTPLHAAAHWGVKEACSILAEALCDMDIRNKLGQTPFDVADEGLVEHLEMLQKKQDVLRSEKETRNKLIESDLNSKFQSGLFKNKEKMLYEEEIPKSQDTEEENKESSSSSSEEEEGEDEVSESETEKEADKKPEATVNHSNSEIKSRIMEQIPAPAQNTFSASSARRLSSLFNKAEEPKDESPSSWRLGLRKTGSHNMLSEVANSREALRDRGSSIYRSSSSPRISALLDDKDKERENKSYFSMLVPRRLSSTSDIEEKENRESAVNLVRSGSHTRQLWRDEAKGSETPQTIAPSTYTSTYLKRTPYKSQADSTAEKTADSVSSSTPLCVITNRPAPSTANGVPAATVFSSAGTDPSVEAREKRRSYLTPVRDEEAESLRKARSRQARQTRRSTQGVTLTDLQEAEKTFSRSRAERQAQEQPGEKLEDPGGLEGSTKKQEPSAAPTKGAGEGRSLEEEPIYHRLRYPTQPDKPTTPVSPSASRPSLYTGSHLLRTSRASGPDSENSETSTHATAAKEMDTSEKGEADLDDQSSNRLSVRERRRAKDRRRGTGINFWTNDEDETDVSEEVKEALHERLSRLESGGTNPTSSDSYSDRASARARREAREARLASLTSRVEEDSNRDYKKLYESALTENQKLKTKLQEAQLELADIKAKLEKMAQQKQEKTSDRSSVLEVEKRERRALERKMSEMEEEMKVLTELKSDNQRLKDENGALIRVISKLSK
InterPro domains: - IPR036770: Ankyrin repeat-containing domain superfamily (homologous_superfamily) [11-317]
- IPR051226: Protein Phosphatase 1 Regulatory Subunit (family) [6-976]
- IPR002110: Ankyrin repeat (repeat) [56-306]
- IPR017401: Protein phosphatase 1 regulatory subunit 12A/B/C (family) [10-976]
- IPR031775: cGMP-dependent protein kinase, interacting domain (domain) [875-976]
Interaction partners: - Serine/threonine-protein phosphatase PP1-beta catalytic subunit
- Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
- Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
- Protein phosphatase 1 regulatory subunit 12C
- Rho-associated protein kinase 1
- Rho-associated protein kinase 2
- Myosin regulatory light polypeptide 9
- Protein phosphatase 1 regulatory subunit 14A
- cGMP-dependent protein kinase 1
- Myosin light polypeptide 6
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0065007 (biological regulation), GO:0051716 (cellular response to stimulus), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0051239 (regulation of multicellular organismal process), GO:0050794 (regulation of cellular process), GO:0044057 (regulation of system process), GO:0090257 (regulation of muscle system process), GO:0006937 (regulation of muscle contraction)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0030017 (sarcomere), GO:0031674 (I band), GO:0030018 (Z disc), GO:0005737 (cytoplasm), GO:0031672 (A band), GO:0099080 (supramolecular complex), GO:0099081 (supramolecular polymer), GO:0043292 (contractile fiber), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0099512 (supramolecular fiber), GO:0030016 (myofibril), GO:0043232 (intracellular non-membrane-bounded organelle)