Protein: Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4 (EC 4.2.1.134) (3-hydroxyacyl-CoA dehydratase 4) (HACD4) (Protein-tyrosine phosphatase-like A domain-containing protein 2) (Q5VWC8)
Organism: Homo sapiens (Human)
Function: Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:18554506}; Multi-pass membrane protein {ECO:0000269|PubMed:18554506}.
Sequence: MGPLALPAWLQPRYRKNAYLFIYYLIQFCGHSWIFTNMTVRFFSFGKDSMVDTFYAIGLVMRLCQSVSLLELLHIYVGIESNHLLPRFLQLTERIIILFVVITSQEEVQEKYVVCVLFVFWNLLDMVRYTYSMLSVIGISYAVLTWLSQTLWMPIYPLCVLAEAFAIYQSLPYFESFGTYSTKLPFDLSIYFPYVLKIYLMMLFIGMYFTYSHLYSERRDILGIFPIKKKKM
InterPro domains: - IPR007482: Protein-tyrosine phosphatase-like, PTPLA (family) [16-223]
Interaction partners: - Very-long-chain enoyl-CoA reductase
- Very-long-chain 3-oxoacyl-CoA reductase
- Very long chain fatty acid elongase 4
- Very-long-chain
- Very long chain fatty acid elongase 2
- Very-long-chain
- Very long chain fatty acid elongase 6
- Stearoyl-CoA desaturase 5
- Stearoyl-CoA desaturase
- Very-long-chain
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0005515 (protein binding), GO:0016829 (lyase activity), GO:0016835 (carbon-oxygen lyase activity), GO:0019899 (enzyme binding), GO:0016836 (hydro-lyase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0035383 (thioester metabolic process), GO:0006793 (phosphorus metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006629 (lipid metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0006082 (organic acid metabolic process), GO:0043603 (amide metabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0043436 (oxoacid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0043604 (amide biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:0035384 (thioester biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0006665 (sphingolipid metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006631 (fatty acid metabolic process), GO:0006637 (acyl-CoA metabolic process), GO:0044272 (sulfur compound biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0019693 (ribose phosphate metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0006643 (membrane lipid metabolic process), GO:0090407 (organophosphate biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:1901568 (fatty acid derivative metabolic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0046467 (membrane lipid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0009117 (nucleotide metabolic process), GO:0035337 (fatty-acyl-CoA metabolic process), GO:0034032 (purine nucleoside bisphosphate metabolic process), GO:1901293 (nucleoside phosphate biosynthetic process), GO:0030148 (sphingolipid biosynthetic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0033865 (nucleoside bisphosphate metabolic process), GO:0009259 (ribonucleotide metabolic process), GO:0006633 (fatty acid biosynthetic process), GO:0019752 (carboxylic acid metabolic process), GO:0046390 (ribose phosphate biosynthetic process), GO:0006163 (purine nucleotide metabolic process), GO:0072522 (purine-containing compound biosynthetic process), GO:0071616 (acyl-CoA biosynthetic process), GO:1901570 (fatty acid derivative biosynthetic process), GO:0000038 (very long-chain fatty acid metabolic process), GO:0009150 (purine ribonucleotide metabolic process), GO:0009260 (ribonucleotide biosynthetic process), GO:0034033 (purine nucleoside bisphosphate biosynthetic process), GO:0046949 (fatty-acyl-CoA biosynthetic process), GO:0033875 (ribonucleoside bisphosphate metabolic process), GO:0042761 (very long-chain fatty acid biosynthetic process), GO:0035336 (long-chain fatty-acyl-CoA metabolic process), GO:0072330 (monocarboxylic acid biosynthetic process), GO:0009165 (nucleotide biosynthetic process), GO:0032787 (monocarboxylic acid metabolic process), GO:0006164 (purine nucleotide biosynthetic process), GO:0030497 (fatty acid elongation), GO:0033866 (nucleoside bisphosphate biosynthetic process), GO:0035338 (long-chain fatty-acyl-CoA biosynthetic process), GO:0034030 (ribonucleoside bisphosphate biosynthetic process), GO:0009152 (purine ribonucleotide biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031984 (organelle subcompartment), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0031090 (organelle membrane), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0043231 (intracellular membrane-bounded organelle), GO:0005789 (endoplasmic reticulum membrane)