Protein: UTP:RNA uridylyltransferase 1 (EC 2.7.7.52) (O64642)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: UTP:RNA uridylyltransferase with a marked preference for uridine polymerization and a distributive activity for the first added nucleotides. Uridylates oligo(A)-tailed mRNAs to prevent 3' to 5' ribonucleotytic attacks. Reduces the accumulation of oligo(A)-tailed mRNAs. Prevents the accumulation of excessively deadenylated mRNAs to avoid siRNA biogenesis. Uridylation repairs deadenylated extremities to restore the size distribution observed for non-uridylated oligo(A) tails. Can prevent the 3' trimming of mRNAs still engaged on polysomes. Acts synergistically with HESO1 in unmethylated miRNA uridylation, leading to their degradation. URT1 and HESO1 prefer substrates with different 3' end nucleotides and act cooperatively to tail different forms of the same miRNAs. URT1 and HESO1 act sequentially, with URT1 mono-uridylating the miRNAs followed by their further uridylation by HESO1. URT1 and HESO1 are involved in the uridylation and clearance of RISC-generated 5' mRNA fragments. Has no effect on uridylation of heterochromatic siRNAs. Able to act on AGO1-bound miRNAs and the uridylated species stay associated with AGO1. Acts as post-transcriptional gene silencing (PTGS) suppressor.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:23748567}. Cytoplasm, P-body {ECO:0000269|PubMed:23748567, ECO:0000269|PubMed:25928341}. Note=The P618L single amino acid substitutiontarget URT1 to the nucleoplasm. {ECO:0000269|PubMed:25928341}.
Sequence: MADGGAEPPAPPSSINAGEFLLSILHGSPSPSSQGPQHHQSFALDPAIAAIGPTVNNPFPPSNWQSNGHRPSNHNPPSWPLAFSPPHNLSPNFLGFPQFPPSPFTTNQFDGNQRVSPEDAYRLGFPGTTNPAIQSMVQQQQQQQLPPPQSETRKLVFGSFSGDATQSLNGLHNGNLKYDSNQHEQLMRHPQSTLSNSNMDPNLSHHRNHDLHEQRGGHSGRGNWGHIGNNGRGLKSTPPPPPPGFSSNQRGWDMSLGSKDDDRGMGRNHDQAMGEHSKVWNQSVDFSAEANRLRGLSIQNESKFNLSQQIDHPGPPKGASLHSVSAADAADSFSMLNKEARRGGERREELGQLSKAKREGNANSDEIEDFGEDIVKSLLLEDETGEKDANDGKKDSKTSREKESRVDNRGQRLLGQKARMVKMYMACRNDIHRYDATFIAIYKSLIPAEEELEKQRQLMAHLENLVAKEWPHAKLYLYGSCANSFGFPKSDIDVCLAIEGDDINKSEMLLKLAEILESDNLQNVQALTRARVPIVKLMDPVTGISCDICINNVLAVVNTKLLRDYAQIDVRLRQLAFIVKHWAKSRRVNETYQGTLSSYAYVLMCIHFLQQRRPPILPCLQEMEPTYSVRVDNIRCTYFDNVDRLRNFGSNNRETIAELVWGFFNYWAYAHDYAYNVVSVRTGSILGKREKDWTRRVGNDRHLICIEDPFETSHDLGRVVDKFSIRVLREEFERAARIMHQDPNPCAKLLEPYIPEDNNGQGHN
InterPro domains: - IPR002058: PAP/25A-associated (domain) [656-714]
- IPR043519: Nucleotidyltransferase superfamily (homologous_superfamily) [434-568]
- IPR054708: Poly(A) RNA polymerase, mitochondrial-like, central palm domain (domain) [439-567]
Interaction partners: - Small RNA degrading nuclease 1
- Inactive exonuclease DIS3L2
- Ribonuclease II, chloroplastic/mitochondrial
- Nucleotidyltransferase
- RmlC-like jelly roll fold protein
- Small RNA degrading nuclease 2
- Nucleotidyltransferase family protein
- Small RNA degrading nuclease 5
- Small RNA 2'-O-methyltransferase
- Decapping 5-like protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0003729 (mRNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009628 (response to abiotic stimulus), GO:0048856 (anatomical structure development), GO:0009653 (anatomical structure morphogenesis), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009892 (negative regulation of metabolic process), GO:0065008 (regulation of biological quality), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0006950 (response to stress), GO:0048523 (negative regulation of cellular process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0001101 (response to acid chemical), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0009414 (response to water deprivation), GO:0043487 (regulation of RNA stability), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:1901700 (response to oxygen-containing compound), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0009894 (regulation of catabolic process), GO:0010035 (response to inorganic substance), GO:1901360 (organic cyclic compound metabolic process), GO:0009415 (response to water), GO:0006139 (nucleobase-containing compound metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0009896 (positive regulation of catabolic process), GO:1902074 (response to salt), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0043488 (regulation of mRNA stability), GO:0051248 (negative regulation of protein metabolic process), GO:0050779 (RNA destabilization), GO:0010468 (regulation of gene expression), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0034248 (regulation of amide metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0031331 (positive regulation of cellular catabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0031329 (regulation of cellular catabolic process), GO:0010467 (gene expression), GO:0010629 (negative regulation of gene expression), GO:0034249 (negative regulation of amide metabolic process), GO:0017148 (negative regulation of translation), GO:0016070 (RNA metabolic process), GO:0061013 (regulation of mRNA catabolic process), GO:0061014 (positive regulation of mRNA catabolic process), GO:0016441 (post-transcriptional gene silencing), GO:0031047 (RNA-mediated gene silencing), GO:0006396 (RNA processing), GO:2000112 (regulation of cellular macromolecule biosynthetic process), GO:0006417 (regulation of translation), GO:1903311 (regulation of mRNA metabolic process), GO:0061157 (mRNA destabilization), GO:2000113 (negative regulation of cellular macromolecule biosynthetic process), GO:1903313 (positive regulation of mRNA metabolic process), GO:0010608 (post-transcriptional regulation of gene expression), GO:0034470 (ncRNA processing), GO:0070918 (regulatory ncRNA processing), GO:0035194 (RNA-mediated post-transcriptional gene silencing), GO:0034660 (ncRNA metabolic process), GO:0035196 (miRNA processing), GO:0035195 (miRNA-mediated gene silencing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0043231 (intracellular membrane-bounded organelle)