Protein: MICAL-like protein 1 (D3ZQL6)
Organism: Rattus norvegicus (Rat)
Function: Probable lipid-binding protein with higher affinity for phosphatidic acid, a lipid enriched in recycling endosome membranes. On endosome membranes, may act as a downstream effector of Rab proteins recruiting cytosolic proteins to regulate membrane tubulation. May be involved in a late step of receptor-mediated endocytosis regulating for instance endocytosed-EGF receptor trafficking. Alternatively, may regulate slow endocytic recycling of endocytosed proteins back to the plasma membrane. May indirectly play a role in neurite outgrowth.
Subcellular location: Recycling endosome membrane {ECO:0000250|UniProtKB:Q8N3F8}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q8N3F8}. Late endosome membrane {ECO:0000250|UniProtKB:Q8N3F8}. Cell projection, cilium membrane {ECO:0000250|UniProtKB:Q8N3F8}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q8N3F8}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:Q8N3F8}. Note=Localization to late endosomes is actin-dependent. Association to tubular recycling endosomes is regulated by RAB35 and ARF6. Interaction with tubulins achors MICALL1 to the centriole. {ECO:0000250|UniProtKB:Q8N3F8}.
Sequence: MAGPRGALLAWCRRQCEGYRGVDIRDLSSSFRDGLAFCAILHRHRPDLLDFQSLSKENVFENNRLAFEVAEKELGIPALLDPNDMVSMSVPDCLSIMTYVSQYYNHFTSSGQAAASPPKPGKDPAAPSPTSTSPAVQPGEEAQGDDLSPDSLSEQGKPQPPSSACAACGQRVHLVQRYLAEGRLYHRHCFRCRQCSSTLVPGSYSSGPEEGTFVCAERCTRLGLGGRSGTRPLSLQKQQPAAAAEAKDGEDSDLSKSVVVVAAEADGLQASSEVQPHTLTKPPLPSKPQDLASPPVSRPTPAPRKASESSALTPPTPRPRSSLQQDGMVEQSVSGDLVNGRLQELPVPKPRGTPKLSERMTAPRKDPPWITLVQTEPKKKPAPLPPSSGPGPLSQASRQVENGGLEEKTQKSPAAEPEPKPYNPFEEEEEEEEASVPAVPSPAPAPPETTPKSLHPWYNITPTSSPKTKKRPAPKAPSASPLVLHASRLSHSEPPSATPSPALSVESLSSESSSHTANTEPSEPPAVPKSSSDPAVHVPGTPGTSANSVTPSAHSSLSSSGELGQPSGEQMPQARTRGSPGTHSTKPFSGATPTPFLLAGDQKSPAPPMGSSSPQMLIKSSCKENPFNRKPSPSTSPTVRKATKGAKPVRPPAPGHGFPLIKRKVQADQYIPEEDIYGEMDSIERQLDALEHSGVLLEEKLRGGANEGSEDDMLVDWFKLIHEKHLLVRRESELIYVFKQQNLEQRQADVEFELRCLLNKPEKDWTDDDRAREKVLMQELMTLIEQRDAIVNCLDEDRQREEEEDKMLETMIKKKDFQREAESDSKKKGKFKTMKVLKLLGNKRDAKSKAPTGKS
InterPro domains: - IPR036872: CH domain superfamily (homologous_superfamily) [2-110]
- IPR050540: F-actin Monooxygenase Mical (family) [7-814]
- IPR022735: bMERB domain (domain) [663-810]
- IPR001715: Calponin homology domain (domain) [2-108]
- IPR001781: Zinc finger, LIM-type (domain) [163-226]
Interaction partners: - Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2
- Ras-related protein Rab-8A
- EH domain-containing protein 1
- Ras-related protein Rab-13
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0043167 (ion binding), GO:0008289 (lipid binding), GO:0005515 (protein binding), GO:0042802 (identical protein binding), GO:0043168 (anion binding), GO:0050839 (cell adhesion molecule binding), GO:0019899 (enzyme binding), GO:0005543 (phospholipid binding), GO:0045296 (cadherin binding), GO:0051020 (GTPase binding), GO:0070300 (phosphatidic acid binding), GO:0031267 (small GTPase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0051234 (establishment of localization), GO:0051641 (cellular localization), GO:0033036 (macromolecule localization), GO:0050794 (regulation of cellular process), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0046907 (intracellular transport), GO:0051668 (localization within membrane), GO:0045184 (establishment of protein localization), GO:0006810 (transport), GO:0071702 (organic substance transport), GO:0006886 (intracellular protein transport), GO:0071705 (nitrogen compound transport), GO:0008104 (protein localization), GO:0015031 (protein transport), GO:0090150 (establishment of protein localization to membrane), GO:0072657 (protein localization to membrane), GO:0006612 (protein targeting to membrane), GO:0006605 (protein targeting)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0042995 (cell projection), GO:0120025 (plasma membrane bounded cell projection), GO:0043005 (neuron projection)