Protein: tRNA ligase (EC 6.5.1.3) (P09880)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: One of the two proteins required for the splicing of precursor tRNA molecules containing introns. The ligation activity requires three enzymatic activities: phosphorylation of the 5' terminus of the 3' half-tRNA in the presence of ATP, opening of the 2'3'-cyclic phosphodiester bond of the 5' half-tRNA leaving a 2'-phosphomonoester and ligation of the two tRNA halves in an ATP-dependent reaction.
Sequence: MPSPYDGKRTVTQLVNELEKAEKLSGRGRAYRRVCDLSHSNKKVISWKFNEWDYGKNTITLPCNARGLFISDDTTNPVIVARGYDKFFNVGEVNFTKWNWIEENCTGPYDVTIKANGCIIFISGLEDGTLVVCSKHSTGPRADVDRNHAEAGEKQLLRQLAAMNINRSDFARMLYTHNVTAVAEYCDDSFEEHILEYPLEKAGLYLHGVNVNKAEFETWDMKDVSQMASKYGFRCVQCITSNTLEDLKKFLDNCSATGSFEGQEIEGFVIRCHLKSTEKPFFFKYKFEEPYLMYRQWREVTKDYISNKSRVFKFRKHKFITNKYLDFAIPILESSPKICENYLKGFGVIELRNKFLQSYGMSGLEILNHEKVAELELKNAIDYDKVDERTKFLIFPISVIGCGKTTTSQTLVNLFPDSWGHIQNDDITGKDKSQLMKKSLELLSKKEIKCVIVDRNNHQFRERKQLFEWLNELKEDYLVYDTNIKVIGVSFAPYDKLSEIRDITLQRVIKRGNNHQSIKWDELGEKKVVGIMNGFLKRYQPVNLDKSPDNMFDLMIELDFGQADSSLTNAKQILNEIHKAYPILVPEIPKDDEIETAFRRSLDYKPTVRKIVGKGNNNQQKTPKLIKPTYISAKIENYDEIIELVKRCIASDAELTEKFKHLLASGKVQKELHITLGHVMSSREKEAKKLWKSYCNRYTDQITEYNNNRIENAQGSGNNQNTQVKTTDKLNFRLEKLCWDEKIIAIVVELSKDKDGCIIDENNEKIKGLCCQNKIPHITLCKLESGVKAVYSNVLCEKVESAEVDENIKVVKLDNSKEFVGSVYLNF
InterPro domains: - IPR015965: tRNA ligase, phosphodiesterase (domain) [565-826]
- IPR012387: tRNA ligase Trl1, fungi (family) [1-827]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [371-642]
- IPR019039: T4 RNA ligase 1-like, N-terminal (domain) [64-292]
- IPR015966: tRNA ligase, kinase domain, fungi (domain) [393-560]
Interaction partners: - tRNA 2'-phosphotransferase
- tRNA-splicing endonuclease subunit SEN34
- tRNA-splicing endonuclease subunit SEN54
- tRNA-splicing endonuclease subunit SEN2
- Serine/threonine-protein kinase/endoribonuclease IRE1
- 2',3'-cyclic-nucleotide 3'-phosphodiesterase
- tRNA-splicing endonuclease subunit SEN15
- Transcriptional activator HAC1
- Endoplasmic reticulum chaperone BiP
- Exportin-T
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0140098 (catalytic activity, acting on RNA), GO:0016886 (ligase activity, forming phosphoric ester bonds)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0008380 (RNA splicing), GO:0034470 (ncRNA processing), GO:0034660 (ncRNA metabolic process), GO:0006399 (tRNA metabolic process), GO:0000394 (RNA splicing, via endonucleolytic cleavage and ligation), GO:0008033 (tRNA processing), GO:0006388 (tRNA splicing, via endonucleolytic cleavage and ligation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)