Protein: ATP-dependent DNA helicase hus2/rqh1 (EC 5.6.2.4) (DNA 3'-5' helicase Rqh1) (Q09811)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Has a role in the repair of UV-induced DNA damage in G2 via recombination-mediated repair. Also has a role in the repair of infrared-induced double DNA strand breaks. Exhibits an ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
Subcellular location: Nucleus {ECO:0000269|PubMed:12724426}.
Sequence: MTVTKTNLNRHLDWFFRESPQKIENVTSPIKTLDFVKVKVSSSDIVVKDSIPHKSKNVFDDFDDGYAIDLTEEHQSSSLNNLKWKDVEGPNILKPIKKIAVPASESEEDFDDVDEEMLRAAEMEVFQSCQPLAVNTADTTVSHSTSSSNVPRSLNKIHDPSRFIKDNDVENRIHVSSASKVASISNTSKPNPIVSENPISATSVSIEIPIKPKELSNNLPFPRLNNNNTNNNNDNNAIEKRDSASPTPSSVSSQISIDFSTWPHQNLLQYLDILRDEKSEISDRIIEVMERYPFSSRFKEWIPKRDILSQKISSVLEVLSNNNNSNNNNGNNGTVPNAKTFFTPPSSITQQVPFPSTIIPESTVKENSTRPYVNSHLVANDKITATPFHSEAVVSPLQSNIRNSDIAEFDEFDIDDADFTFNTTDPINDESGASSDVVVIDDEEDDIENRPLNQALKASKAAVSNASLLQSSSLDRPLLGEMKDKNHKVLMPSLDDPMLSYPWSKEVLGCLKHKFHLKGFRKNQLEAINGTLSGKDVFILMPTGGGKSLCYQLPAVIEGGASRGVTLVISPLLSLMQDQLDHLRKLNIPSLPLSGEQPADERRQVISFLMAKNVLVKLLYVTPEGLASNGAITRVLKSLYERKLLARIVIDEAHCVSHWGHDFRPDYKQLGLLRDRYQGIPFMALTATANEIVKKDIINTLRMENCLELKSSFNRPNLFYEIKPKKDLYTELYRFISNGHLHESGIIYCLSRTSCEQVAAKLRNDYGLKAWHYHAGLEKVERQRIQNEWQSGSYKIIVATIAFGMGVDKGDVRFVIHHSFPKSLEGYYQETGRAGRDGKPAHCIMFYSYKDHVTFQKLIMSGDGDAETKERQRQMLRQVIQFCENKTDCRRKQVLAYFGENFDKVHCRKGCDICCEEATYIKQDMTEFSLQAIKLLKSISGKATLLQLMDIFRGSKSAKIVENGWDRLEGAGVGKLLNRGDSERLFHHLVSEGVFVEKVEANRRGFVSAYVVPGRQTIINSVLAGKRRIILDVKESSSKPDTSSRSLSRSKTLPALREYQLKSTTASVDCSIGTREVDEIYDSQMPPVKPSLIHSRNKIDLEELSGQKFMSEYEIDVMTRCLKDLKLLRSNLMAIDDSRVSSYFTDSVLLSMAKKLPRNVKELKEIHGVSNEKAVNLGPKFLQVIQKFIDEKEQNLEGTELDPSLQSLDTDYPIDTNALSLDHEQGFSDDSDSVYEPSSPIEEGDEEVDGQRKDILNFMNSQSLTQTGSVPKRKSTSYTRPSKSYRHKRGSTSYSRKRKYSTSQKDSRKTSKSANTSFIHPMVKQNYR
InterPro domains: - IPR032284: ATP-dependent DNA helicase RecQ, zinc-binding domain (domain) [849-915]
- IPR002464: DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site (conserved_site) [646-655]
- IPR002121: HRDC domain (domain) [1115-1195]
- IPR044876: HRDC domain superfamily (homologous_superfamily) [1111-1197]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [522-693]
- IPR004589: DNA helicase, ATP-dependent, RecQ type (family) [510-969]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [482-967]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [516-723]
- IPR010997: HRDC-like superfamily (homologous_superfamily) [1120-1196]
- IPR018982: RQC domain (domain) [921-1039]
- IPR001650: Helicase, C-terminal domain-like (domain) [728-876]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [922-1035]
Interaction partners: - DNA topoisomerase 3
- RecQ-mediated genome instability protein 1
- DNA repair protein rhp51
- ATP-dependent DNA helicase srs2
- Exodeoxyribonuclease 1
- Crossover junction endonuclease mus81
- Replication factor A protein 1
- DNA repair and recombination protein rad22
- DNA repair protein rhp57
- Protein kinase rad3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0004386 (helicase activity), GO:0008094 (ATP-dependent activity, acting on DNA), GO:0005515 (protein binding), GO:0140097 (catalytic activity, acting on DNA), GO:0003678 (DNA helicase activity), GO:0043138 (3'-5' DNA helicase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0006259 (DNA metabolic process), GO:0006281 (DNA repair)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0090734 (site of DNA damage), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0035861 (site of double-strand break), GO:0043228 (non-membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus), GO:0005694 (chromosome)