Protein: UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioate ligase (P37773)
Organism: Escherichia coli (strain K12)
Function: Reutilizes the intact tripeptide L-alanyl-gamma-D-glutamyl- meso-diaminopimelate by linking it to UDP-N-acetylmuramate. The enzyme can also use the tetrapeptide L-alanyl-gamma-D-glutamyl-meso-2,6- diaminoheptanedioyl-D-alanine or the pentapeptide L-alanyl-gamma-D- glutamyl-meso-2,6-diaminoheptandioyl-D-alanyl-D-alanine in vivo and in vitro
Subcellular location: Secreted
Sequence: MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEKQGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRDRWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSAHLGESDFFVIEADEYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRIIWPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLVGEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHPTAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHIPWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLAKKAEAAQ
InterPro domains: - IPR000713: Mur ligase, N-terminal catalytic domain (domain) [2-100]
- IPR036565: Mur-like, catalytic domain superfamily (homologous_superfamily) [91-313]
- IPR004101: Mur ligase, C-terminal (domain) [313-436]
- IPR050061: Peptidoglycan biosynthesis MurCDEF (family) [2-454]
- IPR036615: Mur ligase, C-terminal domain superfamily (homologous_superfamily) [312-451]
- IPR013221: Mur ligase, central (domain) [108-291]
- IPR005757: Murein peptide ligase (family) [1-450]
Interaction partners: - Beta-hexosaminidase
- Murein tetrapeptide carboxypeptidase
- UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
- 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD
- Anhydromuropeptide permease
- UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
- D-alanine--D-alanine ligase B
- UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
- UDP-N-acetylenolpyruvoylglucosamine reductase
- UDP-N-acetylmuramoylalanine--D-glutamate ligase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0016874 (ligase activity), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:0005515 (protein binding), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0042802 (identical protein binding), GO:0000166 (nucleotide binding), GO:0032553 (ribonucleotide binding), GO:0016879 (ligase activity, forming carbon-nitrogen bonds), GO:0016881 (acid-amino acid ligase activity), GO:0097216 (guanosine tetraphosphate binding), GO:0032555 (purine ribonucleotide binding), GO:0017076 (purine nucleotide binding), GO:0019001 (guanyl nucleotide binding), GO:0032561 (guanyl ribonucleotide binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0071554 (cell wall organization or biogenesis), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0044036 (cell wall macromolecule metabolic process), GO:0044085 (cellular component biogenesis), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044260 (cellular macromolecule metabolic process), GO:0042546 (cell wall biogenesis), GO:0043170 (macromolecule metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0009273 (peptidoglycan-based cell wall biogenesis), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0044038 (cell wall macromolecule biosynthetic process), GO:0070589 (cellular component macromolecule biosynthetic process), GO:0006022 (aminoglycan metabolic process), GO:0030203 (glycosaminoglycan metabolic process), GO:0006023 (aminoglycan biosynthetic process), GO:0009252 (peptidoglycan biosynthetic process), GO:0006024 (glycosaminoglycan biosynthetic process), GO:0000270 (peptidoglycan metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)