Protein: Shugoshin (Q08490)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Plays a central role in chromosome cohesion during mitosis and meiosis divisions by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. Probably act by protecting REC8 and RAD21 from separase degradation during anaphase. Also acts as a spindle checkpoint component required for sensing tension between sister chromatids during mitosis, its degradation when they separate preventing cell cycle arrest and chromosome loss in anaphase, a time when sister chromatids are no longer under tension.
Subcellular location: Chromosome, centromere {ECO:0000269|PubMed:14752166, ECO:0000269|PubMed:15062096}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:15062096}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:15062096}. Note=Localizes to the centromere during S and M phases, dissociates at the onset of anaphase. Colocalizes with kinetochores. Associates with the spindle pole. {ECO:0000269|PubMed:15062096}.
Sequence: MPKRKIAPNKESSRRTVSHDDLTPQIQEFQNLMDLESQKVENIRQSYSRQNSLLAKDNSILKIKVNSLEKKISQLVQENVTLRSKTSISEAIYRERLSNQLQVIENGIIQRFDEIFYMFENVRKNENLPSSSLRTMLKRTSSRSRSCSLSSPTYSKSYTRLSNHENNLSHESSFNKDDGPDLEPKAKKRKSSRRQSMFVSTSLEPEDETGENEPMMENSSVEVPAESHESAQVEETIDALNPEEENSDSVSNFTNSIIEYSIPEENPTEPEHSSSKLEIFNDSTNMLSTVPSNPLPLPLPGPSATLPTTTSDASTVYPSSSSSTNSHPKTKIKHSMKPPRIELKKKVIDEVMPVSNMSSNSEISFTRTRRTRGKAVDYTLPSLRAKMRRPSEKLVDATTVIDIHDLQVSKRNRETSHKRKSLSQDSIPDEPQLREVVVSKDYGTPKGKKTEDEIHEDTAHLMTTSNNNSNNKNEKKLTSNNSPKKSSPLLDITNKSENKKKSTRTKKLFKNAIVNNLSDENSTTRPSKSSKGTSNNNNNYNNFDNNNSNINNVNNKSVSFRLNEDDLAVFDLFGNGKAVKHQPKTYRTKK
InterPro domains: - IPR011515: Shugoshin, C-terminal (domain) [367-389]
- IPR011516: Shugoshin, N-terminal coiled-coil domain (domain) [43-86]
Interaction partners: - Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
- Spindle assembly checkpoint serine/threonine-protein kinase BUB1
- Separin
- Protein phosphatase PP2A regulatory subunit A
- Serine/threonine-protein phosphatase PP2A-2 catalytic subunit
- Serine/threonine-protein phosphatase PP2A-1 catalytic subunit
- Histone H3
- Meiotic recombination protein REC8
- Inner centromere protein SLI15
- Meiosis-specific protein SPO13
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0060090 (molecular adaptor activity), GO:0030674 (protein-macromolecule adaptor activity), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0000003 (reproduction), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0051641 (cellular localization), GO:0051321 (meiotic cell cycle), GO:0019222 (regulation of metabolic process), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0009892 (negative regulation of metabolic process), GO:0051640 (organelle localization), GO:2000242 (negative regulation of reproductive process), GO:0050794 (regulation of cellular process), GO:1903046 (meiotic cell cycle process), GO:0048523 (negative regulation of cellular process), GO:0048522 (positive regulation of cellular process), GO:0061982 (meiosis I cell cycle process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0090068 (positive regulation of cell cycle process), GO:1903047 (mitotic cell cycle process), GO:0060255 (regulation of macromolecule metabolic process), GO:0051649 (establishment of localization in cell), GO:0045132 (meiotic chromosome segregation), GO:0008608 (attachment of spindle microtubules to kinetochore), GO:0010948 (negative regulation of cell cycle process), GO:0031324 (negative regulation of cellular metabolic process), GO:0045787 (positive regulation of cell cycle), GO:0051726 (regulation of cell cycle), GO:0007059 (chromosome segregation), GO:0140013 (meiotic nuclear division), GO:0051656 (establishment of organelle localization), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0050000 (chromosome localization), GO:0051316 (attachment of spindle microtubules to kinetochore involved in meiotic chromosome segregation), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0016043 (cellular component organization), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0010564 (regulation of cell cycle process), GO:0000278 (mitotic cell cycle), GO:0098813 (nuclear chromosome segregation), GO:0007346 (regulation of mitotic cell cycle), GO:1901989 (positive regulation of cell cycle phase transition), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0140014 (mitotic nuclear division), GO:0006325 (chromatin organization), GO:0007127 (meiosis I), GO:0051303 (establishment of chromosome localization), GO:0010468 (regulation of gene expression), GO:0051315 (attachment of mitotic spindle microtubules to kinetochore), GO:0045143 (homologous chromosome segregation), GO:0051315 (attachment of mitotic spindle microtubules to kinetochore), GO:0045143 (homologous chromosome segregation), GO:0051983 (regulation of chromosome segregation), GO:1901987 (regulation of cell cycle phase transition), GO:0007080 (mitotic metaphase plate congression), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0006996 (organelle organization), GO:0051253 (negative regulation of RNA metabolic process), GO:0000070 (mitotic sister chromatid segregation), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0010629 (negative regulation of gene expression), GO:2001141 (regulation of RNA biosynthetic process), GO:0048285 (organelle fission), GO:0006355 (regulation of DNA-templated transcription), GO:0051276 (chromosome organization), GO:0040029 (epigenetic regulation of gene expression), GO:0006338 (chromatin remodeling), GO:0045814 (negative regulation of gene expression, epigenetic), GO:0051310 (metaphase plate congression), GO:0000819 (sister chromatid segregation), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0007096 (regulation of exit from mitosis), GO:0000280 (nuclear division), GO:0031507 (heterochromatin formation), GO:0045892 (negative regulation of DNA-templated transcription), GO:0070828 (heterochromatin organization), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:0030261 (chromosome condensation), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0001754 (eye photoreceptor cell differentiation), GO:0000122 (negative regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000785 (chromatin), GO:0098687 (chromosomal region), GO:0140513 (nuclear protein-containing complex), GO:0031974 (membrane-enclosed lumen), GO:0099080 (supramolecular complex), GO:0000792 (heterochromatin), GO:0043229 (intracellular organelle), GO:0000776 (kinetochore), GO:0043228 (non-membrane-bounded organelle), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0000775 (chromosome, centromeric region), GO:0000779 (condensed chromosome, centromeric region), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005721 (pericentric heterochromatin), GO:0070013 (intracellular organelle lumen), GO:0005634 (nucleus), GO:0005856 (cytoskeleton), GO:0031981 (nuclear lumen), GO:0005819 (spindle), GO:0005694 (chromosome), GO:0015630 (microtubule cytoskeleton), GO:0000793 (condensed chromosome)