Protein: DNA damage-binding protein 1 (D-DDB1) (Damage-specific DNA-binding protein 1) (Protein piccolo) (Q9XYZ5)
Organism: Drosophila melanogaster (Fruit fly)
Function: Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively (By similarity). Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair (By similarity). The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches (By similarity). Also functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1 (By similarity). Required for degradation of gig. Required for genomic stability in the face of endogenous DNA lesions and for the response to MMS-induced DNA damage. Required for normal wing development.
Subcellular location: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
Sequence: MSHHYVVTAQKPTAVVACLTGNFTSPTDLNLIIARNNQVEIDLVTPEGLRPLKEININGTIAVMRHFRPPDSNKDLLFILTRRYNVMILEARMVNDVITVVTKANGNVSDSVGIPSEGGVIAAIDPKARVIGMCLYQGLFTIIPMDKDASELKATNLRMDELNVYDVEFLHGCLNPTVIVIHKDSDGRHVKSHEINLRDKEFMKIAWKQDNVETEATMLIPVPSPIGGVIVIGRESIVYHDGSNYHAVAPLTFRQSTINCYARVSSNGLRYLLGNMDGQLYMLFLGTAETSKGVTVKDIKVEQLGEISIPECITYLDNGFLYIGARHGDSQLVRLNSEAIDGSYVVPVENFTNLAPILDIAVVDLDRQGQGQIITCSGSFKDGSLRIIRIGIGIQEHACIDLPGIKGMWSLKVGVDESPYENTLVLAFVGHTRILTLSGEEVEETEIPGFASDLQTFLCSNVDYDQLIQVTSDSVRLVSSATKALVAEWRPTGDRTIGVVSCNTTQILVASACDIFYIVIEDGSLREQSRRTLAYEVACLDITPLDETQKKSDLVAVGLWTDISAVILSLPDLETIYTEKLSGEIIPRSILMTTFEGIHYLLCALGDGSMYYFIMDQTTGQLTDKKKVTLGTQPTTLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVNHMCSLNAQAYPDSLALANKNAVILGTIDEIQKLHIRTVPLGEGPRRIAYQESSQTFAVSTLRIDVHGRGGAKPLRNSASTQAQNITCSSNFLPKPGGGNSTAANAEVGQEIDVHNLLVIDQNTFEVLHAHQFVAPETISSLMSAKLGDDPNTYYVVATSLVIPEEPEPKVGRIIIFHYHENKLTQVAETKVDGTCYALVEFNGKVLAGIGSFVRLYEWTNEKELRMECNIQNMIAALFLKAKGDFILVGDLMRSITLLQHKQMEGIFVEIARDCEPKWMRAVEILDDDTFLGSETNGNLFVCQKDSAATTDEERQLLPELARFHLGDTVNVFRHGSLVMQNVGERTTPINGCVLYGTCNGAIGIVTQIPQDFYDFLHGLEERLKKIIKSVGKIEHTYYRNFQINSKVEPSEGFIDGDLIESFLDLSRDKMRDAVQGLELTLNGERKSADVEDVIKIVEDLTRMH
InterPro domains: - IPR004871: Cleavage/polyadenylation specificity factor, A subunit, C-terminal (domain) [791-1100]
- IPR015943: WD40/YVTN repeat-like-containing domain superfamily (homologous_superfamily) [4-1040]
- IPR018846: RSE1/DDB1/CPSF1, first beta-propeller domain (domain) [15-349]
- IPR050358: RSE1/DDB1/CFT1/CPSF1 (family) [3-1117]
- IPR036322: WD40-repeat-containing domain superfamily (homologous_superfamily) [258-523]
Interaction partners: - Cullin-4A
- Protein lethal
- Protein cereblon
- DET1- and DDB1-associated protein 1
- Abnormal oocyte
- RING-box protein 1A
- Cullin homolog 1
- DDB1- and CUL4-associated factor 11 homolog
- COP9 signalosome complex subunit 5
- Protein mahjong
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0060090 (molecular adaptor activity), GO:0044877 (protein-containing complex binding), GO:0030674 (protein-macromolecule adaptor activity), GO:0005515 (protein binding), GO:0097602 (cullin family protein binding), GO:0019904 (protein domain specific binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0006259 (DNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0006281 (DNA repair)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0140535 (intracellular protein-containing complex), GO:1990234 (transferase complex), GO:0000151 (ubiquitin ligase complex), GO:0031461 (cullin-RING ubiquitin ligase complex), GO:0080008 (Cul4-RING E3 ubiquitin ligase complex)