Protein: DNA replication licensing factor MCM6 (EC 3.6.4.12) (p105MCM) (Q14566)
Organism: Homo sapiens (Human)
Function: Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Subcellular location: Nucleus {ECO:0000269|PubMed:16899510}. Chromosome {ECO:0000269|PubMed:16899510}. Note=Binds to chromatin during G1 and detaches from it during S phase. {ECO:0000269|PubMed:16899510}.
Sequence: MDLAAAAEPGAGSQHLEVRDEVAEKCQKLFLDFLEEFQSSDGEIKYLQLAEELIRPERNTLVVSFVDLEQFNQQLSTTIQEEFYRVYPYLCRALKTFVKDRKEIPLAKDFYVAFQDLPTRHKIRELTSSRIGLLTRISGQVVRTHPVHPELVSGTFLCLDCQTVIRDVEQQFKYTQPNICRNPVCANRRRFLLDTNKSRFVDFQKVRIQETQAELPRGSIPRSLEVILRAEAVESAQAGDKCDFTGTLIVVPDVSKLSTPGARAETNSRVSGVDGYETEGIRGLRALGVRDLSYRLVFLACCVAPTNPRFGGKELRDEEQTAESIKNQMTVKEWEKVFEMSQDKNLYHNLCTSLFPTIHGNDEVKRGVLLMLFGGVPKTTGEGTSLRGDINVCIVGDPSTAKSQFLKHVEEFSPRAVYTSGKASSAAGLTAAVVRDEESHEFVIEAGALMLADNGVCCIDEFDKMDVRDQVAIHEAMEQQTISITKAGVKATLNARTSILAAANPISGHYDRSKSLKQNINLSAPIMSRFDLFFILVDECNEVTDYAIARRIVDLHSRIEESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKHLRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCCDEVQPKHVKEAFRLLNKSIIRVETPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYNEDINQESAPKASLRLGFSEYCRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRLTHYDHVLIELTQAGLKGSTEGSESYEEDPYLVVNPNYLLED
InterPro domains: - IPR008049: DNA replication licensing factor Mcm6 (family) [83-301]
- IPR031327: Mini-chromosome maintenance protein (family) [20-761]
- IPR027925: MCM, N-terminal domain (domain) [27-115]
- IPR033762: MCM OB domain (domain) [122-252]
- IPR041562: MCM, AAA-lid domain (domain) [571-656]
- IPR041024: Mcm6, C-terminal winged-helix domain (domain) [713-817]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [332-671]
- IPR018525: Mini-chromosome maintenance, conserved site (conserved_site) [455-463]
- IPR001208: MCM domain (domain) [334-556]
- IPR012340: Nucleic acid-binding, OB-fold (homologous_superfamily) [29-253]
Interaction partners: - DNA replication complex GINS protein SLD5
- Protein MCM10 homolog
- DNA replication licensing factor MCM4
- DNA replication licensing factor MCM5
- Cell division cycle 7-related protein kinase
- DNA replication licensing factor MCM7
- Cell division control protein 6 homolog
- DNA replication licensing factor MCM3
- DNA replication complex GINS protein PSF3
- Cell division control protein 45 homolog
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:0004386 (helicase activity), GO:1901363 (heterocyclic compound binding), GO:0008094 (ATP-dependent activity, acting on DNA), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003678 (DNA helicase activity), GO:0140097 (catalytic activity, acting on DNA), GO:0003677 (DNA binding), GO:0003697 (single-stranded DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006259 (DNA metabolic process), GO:0006996 (organelle organization), GO:0090304 (nucleic acid metabolic process), GO:0006260 (DNA replication), GO:0051276 (chromosome organization), GO:0071103 (DNA conformation change), GO:0006261 (DNA-templated DNA replication), GO:0006268 (DNA unwinding involved in DNA replication), GO:0032392 (DNA geometric change), GO:0032508 (DNA duplex unwinding)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0032993 (protein-DNA complex), GO:0140513 (nuclear protein-containing complex), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0031261 (DNA replication preinitiation complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0071162 (CMG complex), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005694 (chromosome), GO:0005634 (nucleus), GO:0000228 (nuclear chromosome)