Protein: ADP-ribosylation factor 1 (EC 3.6.5.2) (P61209)
Organism: Drosophila melanogaster (Fruit fly)
Function: Small GTPase involved in protein trafficking between different compartments. Modulates vesicle budding and uncoating within the Golgi complex. In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles (By similarity). Has a role in eye development. Required for cleavage furrow ingression in embryonic cells.
Subcellular location: Golgi apparatus membrane {ECO:0000269|PubMed:27535433}; Lipid-anchor {ECO:0000250|UniProtKB:P84077}; Cytoplasmic side {ECO:0000305}. Cytoplasm, cytosol {ECO:0000269|PubMed:27535433}. Note=In the GDP-bound form, associates transiently with the membranes via its myristoylated N-terminus where guanine nucleotide-exchange factor (GEF)-mediated nucleotide exchange occurs (By similarity). Following nucleotide exchange, the GTP-bound form undergoes a conformational change, leading to the exposure of a myristoylated N-terminal amphipathic helix that provides stable membrane anchorage (By similarity). Localization to the Golgi is dependent on Asap (PubMed:27535433). {ECO:0000250|UniProtKB:P84080, ECO:0000269|PubMed:27535433}.
Sequence: MGNVFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIGEAREELMRMLAEDELRDAVLLIFANKQDLPNAMNAAEITDKLGLHSLRNRNWYIQATCATSGDGLYEGLDWLSNQLKNANR
InterPro domains: - IPR024156: Small GTPase superfamily, ARF type (family) [1-177]
- IPR005225: Small GTP-binding domain (domain) [16-159]
- IPR006689: Small GTPase superfamily, ARF/SAR type (family) [1-177]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [5-182]
- IPR045872: ADP-ribosylation factor 1-5 (family) [18-176]
Interaction partners: - Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
- Golgi microtubule-associated protein, isoform D
- ADP-ribosylation factor GTPase-activating protein 1
- AP-1 complex subunit gamma
- Coatomer subunit gamma
- Coatomer subunit beta
- ADP ribosylation factor 4
- ADP-ribosylation factor 6
- Phosphatidylinositol 4-kinase beta
- Inositol phosphatase interacting protein, isoform A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0051179 (localization), GO:0051234 (establishment of localization), GO:0051641 (cellular localization), GO:0051301 (cell division), GO:0071840 (cellular component organization or biogenesis), GO:0022402 (cell cycle process), GO:0033036 (macromolecule localization), GO:0007049 (cell cycle), GO:0032506 (cytokinetic process), GO:0070727 (cellular macromolecule localization), GO:0000910 (cytokinesis), GO:0016043 (cellular component organization), GO:1903047 (mitotic cell cycle process), GO:0006810 (transport), GO:0000278 (mitotic cell cycle), GO:0045184 (establishment of protein localization), GO:0071702 (organic substance transport), GO:0016192 (vesicle-mediated transport), GO:0071705 (nitrogen compound transport), GO:0008104 (protein localization), GO:0061640 (cytoskeleton-dependent cytokinesis), GO:1902410 (mitotic cytokinetic process), GO:0015031 (protein transport), GO:0000281 (mitotic cytokinesis), GO:0061024 (membrane organization), GO:0010324 (membrane invagination), GO:0099024 (plasma membrane invagination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0030054 (cell junction), GO:0032153 (cell division site), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:1990234 (transferase complex), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0045202 (synapse), GO:0032154 (cleavage furrow), GO:0005938 (cell cortex), GO:0098590 (plasma membrane region), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0055037 (recycling endosome), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0012506 (vesicle membrane), GO:0010008 (endosome membrane), GO:0031982 (vesicle), GO:0030659 (cytoplasmic vesicle membrane), GO:0097708 (intracellular vesicle), GO:0055038 (recycling endosome membrane)