Protein: Nucleoporin NUP49/NSP49 (Nuclear pore protein NUP49/NSP49) (Q02199)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP49 plays an important role in several nuclear transport pathways including poly(A)+ RNA, tRNA, and pre-ribosome transport.
Subcellular location: Nucleus, nuclear pore complex {ECO:0000269|PubMed:10684247}. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note=Symmetric distribution.
Sequence: MFGLNKASSTPAGGLFGQASGASTGNANTGFSFGGTQTGQNTGPSTGGLFGAKPAGSTGGLGASFGQQQQQSQTNAFGGSATTGGGLFGNKPNNTANTGGGLFGANSNSNSGSLFGSNNAQTSRGLFGNNNTNNINNSSSGMNNASAGLFGSKPAGGTSLFGNTSTSSAPAQNQGMFGAKPAGTSLFGNNAGNTTTGGGLFGSKPTGATSLFGSSNNNNNNNNSNNIMSASGGLFGNQQQQLQQQPQMQCALQNLSQLPITPMTRISELPPQIRQEIEQLDQYIQKQVQISHHLKADTIDHDELIDSIPRDVAYLLKSESATSQYLKQDLKKISSFKSLIDEDLLDTQTFSVLLQQLLTPGSKISSNDLDKFFQKKIHLYEKKLEDYCRILSDIETAVNGIDTDLFGAPNNPNSTAITADLGSSEAENLLQLKTGLAAIVSTVIEEFTLFMDIAERIAVLHQKTKTLASLSI
InterPro domains: - IPR024882: Nucleoporin p58/p45/NUP49 (family) [140-467]
- IPR025574: Nucleoporin FG repeat (repeat) [64-246]
Interaction partners: - Nucleoporin NUP2
- Nucleoporin NUP57
- Nucleoporin NUP159
- Nucleoporin NUP133
- Nucleoporin NIC96
- Nucleoporin NSP1
- Nucleoporin ASM4
- Nucleoporin NUP85
- Nucleoporin NUP192
- Nucleoporin NUP145
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005198 (structural molecule activity), GO:0005488 (binding), GO:0017056 (structural constituent of nuclear pore), GO:0005515 (protein binding), GO:0042802 (identical protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0031503 (protein-containing complex localization), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0051640 (organelle localization), GO:0033036 (macromolecule localization), GO:0044085 (cellular component biogenesis), GO:0046907 (intracellular transport), GO:0006403 (RNA localization), GO:0051656 (establishment of organelle localization), GO:0006810 (transport), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0033750 (ribosome localization), GO:0051236 (establishment of RNA localization), GO:0043170 (macromolecule metabolic process), GO:0045184 (establishment of protein localization), GO:0000054 (ribosomal subunit export from nucleus), GO:0050658 (RNA transport), GO:0006886 (intracellular protein transport), GO:0071705 (nitrogen compound transport), GO:0015031 (protein transport), GO:0072594 (establishment of protein localization to organelle), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0071702 (organic substance transport), GO:0051169 (nuclear transport), GO:0000055 (ribosomal large subunit export from nucleus), GO:0008104 (protein localization), GO:0010467 (gene expression), GO:0006606 (protein import into nucleus), GO:0015931 (nucleobase-containing compound transport), GO:0006913 (nucleocytoplasmic transport), GO:0042254 (ribosome biogenesis), GO:0033365 (protein localization to organelle), GO:0051031 (tRNA transport), GO:0051028 (mRNA transport), GO:0006406 (mRNA export from nucleus), GO:0006405 (RNA export from nucleus), GO:0034504 (protein localization to nucleus), GO:0051170 (import into nucleus), GO:0016973 (poly(A)+ mRNA export from nucleus), GO:0097064 (ncRNA export from nucleus), GO:0051168 (nuclear export), GO:0050657 (nucleic acid transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0140513 (nuclear protein-containing complex), GO:0012505 (endomembrane system), GO:0031967 (organelle envelope), GO:0005635 (nuclear envelope), GO:0005643 (nuclear pore), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)