Protein: H/ACA ribonucleoprotein complex subunit 4 (EC 5.4.99.-) (Nucleolar protein at 60B) (Protein minifly) (O44081)
Organism: Drosophila melanogaster (Fruit fly)
Function: Plays a central role in ribosomal RNA processing. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs. Required for maintenance of the germline stem cell lineage during spermatogenesis.
Subcellular location: Nucleus, nucleolus {ECO:0000269|PubMed:10087258, ECO:0000269|PubMed:37343092, ECO:0000269|PubMed:9829824}.
Sequence: MADVEVRKEKKKKKIKEEPLDGDDIGTLQKQGNFQIKPSSKIAELDTSQWPLLLKNFDKLNIRSNHYTPLAHGSSPLNRDIKEYMKTGFINLDKPSNPSSHEVVAWIKKILKVEKTGHSGTLDPKVTGCLIVCIDRATRLVKSQQSAGKEYVAIFKLHGAVESVAKVRQGLEKLRGALFQRPPLISAVKRQLRVRTVYDSKLLDYDETRNMGVFWVSCEAGSYIRTMCVHLGLVLGVGGQMLELRRVRSGIQSERDGMVTMHDVLDAMWLYENHKDESMLRRVIKPLEGLLVNHKRIIMKDSSVNAVCYGAKITLPGVLRYEDGIEIDQEIVICTTKGEAICLAIALMTTATMASCDHGVVAKIKRVIMERDTYPRKWGLGPKASAKKALIAAGKLDKFGRPNENTPKEWLTGYVDYNAKKPAAQEVSPTNGSSEPSKRKLSTSSVEETAAAAVSEETPSKDKKKKKKKHKGDEEAPEAAEEEAEPVEKEKKKKKKKDKDRDRDEAQE
InterPro domains: - IPR004802: tRNA pseudouridine synthase B family (family) [10-503]
- IPR020103: Pseudouridine synthase, catalytic domain superfamily (homologous_superfamily) [47-292]
- IPR032819: tRNA pseudouridylate synthase B, C-terminal (domain) [225-291]
- IPR036974: PUA domain superfamily (homologous_superfamily) [47-378]
- IPR012960: Dyskerin-like (domain) [46-104]
- IPR002501: Pseudouridine synthase II, N-terminal (domain) [108-224]
- IPR002478: PUA domain (domain) [295-369]
- IPR015947: PUA-like superfamily (homologous_superfamily) [294-375]
Interaction partners: - H/ACA ribonucleoprotein complex subunit 3
- H/ACA ribonucleoprotein complex subunit 2
- H/ACA ribonucleoprotein complex subunit 1
- Nucleolar protein 56
- Protein SHQ1 homolog
- Nucleolar protein 58
- rRNA 2'-O-methyltransferase fibrillarin
- H/ACA ribonucleoprotein complex non-core subunit NAF1
- rRNA 2'-O-methyltransferase fibrillarin
- Probable ATP-dependent RNA helicase pitchoune
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0010467 (gene expression), GO:0042254 (ribosome biogenesis), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0034470 (ncRNA processing), GO:0006364 (rRNA processing), GO:0034660 (ncRNA metabolic process), GO:0016072 (rRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus)