Protein: 3-dehydroquinate dehydratase (3-dehydroquinase) (EC 4.2.1.10) (Type I DHQase) (Type I dehydroquinase) (DHQ1) (Q186A6)
Organism: Clostridioides difficile (strain 630) (Peptoclostridium difficile)
Function: Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate. The reaction involves the formation of an imine intermediate between the keto group of 3-dehydroquinate and the epsilon-amino group of Lys-170 at the active site.
Sequence: MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLDIIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLNKEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLCRMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGEIFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN
InterPro domains: - IPR013785: Aldolase-type TIM barrel (homologous_superfamily) [1-255]
- IPR018508: 3-dehydroquinate dehydratase, active site (active_site) [115-145]
- IPR001381: 3-dehydroquinate dehydratase type I (family) [18-250]
- IPR050146: Type-I 3-dehydroquinase (family) [17-252]
Interaction partners: - Shikimate dehydrogenase
- Shikimate dehydrogenase
- 3-dehydroquinate synthase
- Shikimate kinase
- Transporter, Major Facilitator Superfamily
- Dihydroxy-acid dehydratase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016829 (lyase activity), GO:0016835 (carbon-oxygen lyase activity), GO:0016836 (hydro-lyase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0016053 (organic acid biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0042537 (benzene-containing compound metabolic process), GO:0018958 (phenol-containing compound metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0009712 (catechol-containing compound metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0046189 (phenol-containing compound biosynthetic process), GO:0072330 (monocarboxylic acid biosynthetic process), GO:0009713 (catechol-containing compound biosynthetic process), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)