Protein: 2-isopropylmalate synthase (EC 2.3.3.13) (Alpha-IPM synthase) (Alpha-isopropylmalate synthase) (P9WQB3)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (alpha-ketoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate). Active on small alpha-keto acids such as 3-methyl-2-oxobutanoate, pyruvate, alpha-ketovalerate, and alpha-ketobutyrate; only uses acetyl-CoA. Complements an E.coli deletion.
Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00572}.
Sequence: MTTSESPDAYTESFGAHTIVKPAGPPRVGQPSWNPQRASSMPVNRYRPFAEEVEPIRLRNRTWPDRVIDRAPLWCAVDLRDGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSGAPRAIVHFYNSTSILQRRVVFRANRAEVQAIATDGARKCVEQAAKYPGTQWRFEYSPESYTGTELEYAKQVCDAVGEVIAPTPERPIIFNLPATVEMTTPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELGFAAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPVHERHPYGGDLVYTAFSGSHQDAINKGLDAMKLDADAADCDVDDMLWQVPYLPIDPRDVGRTYEAVIRVNSQSGKGGVAYIMKTDHGLSLPRRLQIEFSQVIQKIAEGTAGEGGEVSPKEMWDAFAEEYLAPVRPLERIRQHVDAADDDGGTTSITATVKINGVETEISGSGNGPLAAFVHALADVGFDVAVLDYYEHAMSAGDDAQAAAYVEASVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTSKTVWGVGIAPSITTASLRAVVSAVNRAAR
InterPro domains: - IPR054692: 2-isopropylmalate synthase-like, post-catalytic domain (domain) [369-442]
- IPR013709: 2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain (domain) [474-643]
- IPR002034: Alpha-isopropylmalate/homocitrate synthase, conserved site (conserved_site) [79-295]
- IPR036230: 2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamily (homologous_superfamily) [426-642]
- IPR005668: 2-isopropylmalate synthase (family) [42-644]
- IPR039371: LeuA, N-terminal catalytic TIM barrel domain (domain) [71-354]
- IPR013785: Aldolase-type TIM barrel (homologous_superfamily) [49-425]
- IPR000891: Pyruvate carboxyltransferase (domain) [72-352]
Interaction partners: - 3-isopropylmalate dehydratase large subunit
- 3-isopropylmalate dehydratase small subunit
- Dihydroxy-acid dehydratase
- Branched-chain-amino-acid aminotransferase
- Propanal dehydrogenase
- Putative acetolactate synthase small subunit
- Acetolactate synthase large subunit IlvB1
- Acetolactate synthase large subunit IlvG
- Putative acetolactate synthase large subunit IlvX
- Biotin-dependent acyl-coenzyme A carboxylase alpha3 subunit
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016746 (acyltransferase activity), GO:0046912 (acyltransferase activity, acyl groups converted into alkyl on transfer)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009081 (branched-chain amino acid metabolic process), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0006551 (leucine metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0009082 (branched-chain amino acid biosynthetic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0009098 (leucine biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0005886 (plasma membrane), GO:0009274 (peptidoglycan-based cell wall)