Protein: Biotin synthase (EC 2.8.1.6) (Biotin synthetase) (P12996)
Organism: Escherichia coli (strain K12)
Function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.
Sequence: MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVSTLLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAWKNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEFYGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPESVPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMCFMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMTPDTDEYYNAAAL
InterPro domains: - IPR010722: Biotin and thiamin synthesis-associated domain (domain) [220-312]
- IPR007197: Radical SAM (domain) [38-256]
- IPR002684: Biotin synthase/Biotin biosynthesis bifunctional protein BioAB (family) [3-327]
- IPR006638: Elp3/MiaA/NifB-like, radical SAM core domain (domain) [43-255]
- IPR013785: Aldolase-type TIM barrel (homologous_superfamily) [4-315]
- IPR024177: Biotin synthase (family) [1-319]
Interaction partners: - Adenosylmethionine-8-amino-7-oxononanoate aminotransferase
- ATP-dependent dethiobiotin synthetase BioD 1
- 8-amino-7-oxononanoate synthase
- Malonyl-[acyl-carrier protein] O-methyltransferase
- ATP-dependent dethiobiotin synthetase BioD 2
- Bifunctional ligase/repressor BirA
- Biotin sulfoxide reductase
- tRNA-2-methylthio-N
- Lipoyl synthase
- L-lysine 2,3-aminomutase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016782 (transferase activity, transferring sulphur-containing groups), GO:0016783 (sulfurtransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0006793 (phosphorus metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0019693 (ribose phosphate metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0090407 (organophosphate biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0009259 (ribonucleotide metabolic process), GO:0009117 (nucleotide metabolic process), GO:0046390 (ribose phosphate biosynthetic process), GO:0006163 (purine nucleotide metabolic process), GO:0034032 (purine nucleoside bisphosphate metabolic process), GO:0072522 (purine-containing compound biosynthetic process), GO:1901293 (nucleoside phosphate biosynthetic process), GO:0033865 (nucleoside bisphosphate metabolic process), GO:0015936 (coenzyme A metabolic process), GO:0009165 (nucleotide biosynthetic process), GO:0006164 (purine nucleotide biosynthetic process), GO:0009150 (purine ribonucleotide metabolic process), GO:0009260 (ribonucleotide biosynthetic process), GO:0034033 (purine nucleoside bisphosphate biosynthetic process), GO:0033866 (nucleoside bisphosphate biosynthetic process), GO:0033875 (ribonucleoside bisphosphate metabolic process), GO:0009152 (purine ribonucleotide biosynthetic process), GO:0015937 (coenzyme A biosynthetic process), GO:0034030 (ribonucleoside bisphosphate biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)