Protein: tRNA pseudouridine synthase 1 (B6JUY9)
Organism: Schizosaccharomyces japonicus (strain yFS275 / FY16936) (Fission yeast)
Function: Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron- containing tRNA(Tyr). Catalyzes pseudouridylation at position 44 in U2 snRNA. Also catalyzes pseudouridylation of mRNAs
Subcellular location: Nucleus
Sequence: MGRGGKRSWHSGDRREAKRNRRNAVYNGEGRPADLVVGEKKPKRKVACLVGYCGTGYHGMQLNPPNKTIEGDLFEAFVKVGAVSSYNADDPKKVALARAARTDKGVHAAGNVITLKLILEDENLIQKVNEVLPPTIRVWDVIRTINSFNPRTYCESRIYEYLLPSYAFAPPKPTSNLGKLIIDNAVSKGDKLPEGDAAEEDIDNLSRRLFFEEGKQLWDEFDKTLSKLKDDFNADPASFKDPYRESLNEADALYDIPQDTKLSRVLKHAEIHELRKYRITPERLRVAREAFKRFEGVHNFHNFTVGQSFHDRSSNRVIKSFTVSDPIIIEGTEWLSLKVHGQSFMLHQIRKMVALIMLVVRTGCPLGRIGETFRKPRVNIPRGPGLGLLLESPFFQGYNEHKAAENNRPPIDFTKYDETIEAFKKEYIYSHIFKEEAASQVFHGFLTFIDSYIGDTFAYLSDRGIETADKTEAEELPKLPKEISSEDEDDEQAAAGNKAELEG
InterPro domains: - IPR020095: Pseudouridine synthase I, TruA, C-terminal (homologous_superfamily) [253-458]
- IPR001406: Pseudouridine synthase I, TruA (family) [31-441]
- IPR020094: Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal (homologous_superfamily) [37-154]
- IPR020097: Pseudouridine synthase I, TruA, alpha/beta domain (domain) [290-373]
- IPR041708: Pseudouridine synthase PUS1/ PUS2-like (family) [48-395]
- IPR020103: Pseudouridine synthase, catalytic domain superfamily (homologous_superfamily) [42-401]
Interaction partners: - H/ACA ribonucleoprotein complex subunit CBF5
- tRNA pseudouridine(55) synthase
- Multifunctional tryptophan biosynthesis protein
- tRNA (Guanine-N7-)-methyltransferase subunit Trm82
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016853 (isomerase activity), GO:0016866 (intramolecular transferase activity), GO:0009982 (pseudouridine synthase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0009451 (RNA modification), GO:0034470 (ncRNA processing), GO:0006400 (tRNA modification), GO:0001522 (pseudouridine synthesis), GO:0034660 (ncRNA metabolic process), GO:0031119 (tRNA pseudouridine synthesis), GO:0006399 (tRNA metabolic process), GO:0016073 (snRNA metabolic process), GO:0008033 (tRNA processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)