Protein: Ubiquitin-conjugating enzyme E2 J1 (Q9Y385)
Organism: Homo sapiens (Human)
Function: Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD) and is essential for cells to recover from ER stress. Plays a role in MAPKAPK2-dependent translational control of TNF-alpha synthesis. Also acts as a platform for perinuclear positioning of the endosomal system by mediating ubiquitination of SQSTM1 through interaction with the E3 ubiquitin-protein ligase RNF26. Plays a role in male fecundity through the interaction with the E3 ubiquitin-protein ligase RNF133
Subcellular location: Endoplasmic reticulum membrane; Single-pass type IV membrane protein
Sequence: METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSGSDSSQADQEAKELARQISFKAEVNSSGKTISESDLNHSFSLTDLQDDIPTTFQGATASTSYGLQNSSAASFHQPTQPVAKNTSMSPRQRRAQQQSQRRLSTSPDVIQGHQPRDNHTDHGGSAVLIVILTLALAALIFRRIYLANEYIFDFEL
InterPro domains: - IPR016135: Ubiquitin-conjugating enzyme/RWD-like (homologous_superfamily) [2-184]
- IPR000608: Ubiquitin-conjugating (UBC), catalytic core domain (domain) [10-160]
- IPR050113: Ubiquitin-conjugating enzyme (family) [9-122]
Interaction partners: - Protein sel-1 homolog 1
- E3 ubiquitin-protein ligase RNF5
- E3 ubiquitin-protein ligase synoviolin
- Protein OS-9
- Ubiquitin-conjugating enzyme E2 G2
- Derlin-2
- Lipid droplet-regulating VLDL assembly factor AUP1
- FAS-associated factor 2
- Ubiquitin-conjugating enzyme E2 G1
- Ubiquitin-conjugating enzyme E2 G1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0019899 (enzyme binding), GO:0019787 (ubiquitin-like protein transferase activity), GO:0061650 (ubiquitin-like protein conjugating enzyme activity), GO:0004842 (ubiquitin-protein transferase activity), GO:0044389 (ubiquitin-like protein ligase binding), GO:0031625 (ubiquitin protein ligase binding), GO:0061631 (ubiquitin conjugating enzyme activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0009628 (response to abiotic stimulus), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0009892 (negative regulation of metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0048523 (negative regulation of cellular process), GO:0007154 (cell communication), GO:0044238 (primary metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0009314 (response to radiation), GO:0044248 (cellular catabolic process), GO:0019538 (protein metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0009991 (response to extracellular stimulus), GO:1901360 (organic cyclic compound metabolic process), GO:0104004 (cellular response to environmental stimulus), GO:0006139 (nucleobase-containing compound metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0071214 (cellular response to abiotic stimulus), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0031668 (cellular response to extracellular stimulus), GO:0080090 (regulation of primary metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0071496 (cellular response to external stimulus), GO:0071478 (cellular response to radiation), GO:0009416 (response to light stimulus), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0030163 (protein catabolic process), GO:0010468 (regulation of gene expression), GO:0036211 (protein modification process), GO:0009057 (macromolecule catabolic process), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0044265 (cellular macromolecule catabolic process), GO:0031667 (response to nutrient levels), GO:0031326 (regulation of cellular biosynthetic process), GO:0006259 (DNA metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0051253 (negative regulation of RNA metabolic process), GO:0031669 (cellular response to nutrient levels), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0070647 (protein modification by small protein conjugation or removal), GO:0006355 (regulation of DNA-templated transcription), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0051603 (proteolysis involved in protein catabolic process), GO:0071482 (cellular response to light stimulus), GO:0009411 (response to UV), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0036503 (ERAD pathway), GO:0010498 (proteasomal protein catabolic process), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0034644 (cellular response to UV), GO:0043161 (proteasome-mediated ubiquitin-dependent protein catabolic process), GO:0045892 (negative regulation of DNA-templated transcription), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0032446 (protein modification by small protein conjugation), GO:0034644 (cellular response to UV), GO:0019941 (modification-dependent protein catabolic process), GO:0006511 (ubiquitin-dependent protein catabolic process), GO:0000122 (negative regulation of transcription by RNA polymerase II), GO:0016567 (protein ubiquitination), GO:0051865 (protein autoubiquitination), GO:0000209 (protein polyubiquitination), GO:0070936 (protein K48-linked ubiquitination), GO:0070534 (protein K63-linked ubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031984 (organelle subcompartment), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0031090 (organelle membrane), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0043231 (intracellular membrane-bounded organelle), GO:0005789 (endoplasmic reticulum membrane)