Protein: Phosphatidylinositol transfer protein CSR1 (CHS5 SPA2 rescue protein 1) (SEC14 homolog protein 2) (Q06705)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Non-classical phosphatidylinositol (PtdIns) transfer protein (PITP), which exhibits PtdIns-binding/transfer activity in the absence of detectable PtdCho-binding/transfer activity. Activates SPO14/PLD1 (phospholipase D1) by stimulating phosphoinositide synthesis via the STT4 PtdIns 4-kinase. Modulates ArfGAP function through effects on SPO14 activity. Inhibits phosphatidylcholine degradation by PLB1 (phospholipase B1). May also regulate post-Golgi membrane-trafficking events and have a role resistance to oxidative stress. Inhibits fatty acid synthase activity in response to heme depletion and oleic acid starvation, preventing saturated fatty acid (SFA) accumulation.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:12869188}. Microsome {ECO:0000269|PubMed:12869188}. Endosome {ECO:0000269|PubMed:10848624}.
Sequence: MSFDRQLTEDQEVVLKQIWTHLFHLWQVPVDGTHIFPNNSLHSSSTPAKKKKSSWFSKLQSSDHTQDSSEAAEAAHLYEKGKIHKALANLDPQTTKKQFWHDIKNETPDATILKFIRARKWNADKTIAMLGHDLYWRKDTINKIINGGERAVYENNETGVIKNLELQKATIQGYDNDMRPVILVRPRLHHSSDQTEQELEKFSLLVIEQSKLFFKENYPASTTILFDLNGFSMSNMDYAPVKFLITCFEAHYPESLGHLLIHKAPWIFNPIWNIIKNWLDPVVASKIVFTKNIDELHKFIQPQYIPRYLGGENDNDLDHYTPPDGSLDVHLKDTETRAMIEKEREELVEQFLTVTAQWIEHQPLNDPAYIQLQEKRVQLSTALCENYSKLDPYIRSRSVYDYNGSLKV
InterPro domains: - IPR001251: CRAL-TRIO lipid binding domain (domain) [157-317]
- IPR011074: CRAL/TRIO, N-terminal domain (domain) [83-133]
- IPR036273: CRAL/TRIO, N-terminal domain superfamily (homologous_superfamily) [5-139]
- IPR052432: Phosphatidylinositol transfer protein/CRAL-TRIO domain (family) [6-407]
- IPR036865: CRAL-TRIO lipid binding domain superfamily (homologous_superfamily) [35-350]
Interaction partners: - Phosphatidylinositol transfer protein SFH5
- SEC14 cytosolic factor
- Putative uncharacterized protein YLR379W
- Zinc-regulated transporter 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0005488 (binding), GO:0030234 (enzyme regulator activity), GO:0140677 (molecular function activator activity), GO:0008289 (lipid binding), GO:0043167 (ion binding), GO:0005515 (protein binding), GO:0005543 (phospholipid binding), GO:0043168 (anion binding), GO:0008047 (enzyme activator activity), GO:0035091 (phosphatidylinositol binding), GO:0080025 (phosphatidylinositol-3,5-bisphosphate binding), GO:0005546 (phosphatidylinositol-4,5-bisphosphate binding), GO:0070300 (phosphatidic acid binding), GO:1901981 (phosphatidylinositol phosphate binding), GO:0032266 (phosphatidylinositol-3-phosphate binding), GO:1902936 (phosphatidylinositol bisphosphate binding), GO:0010314 (phosphatidylinositol-5-phosphate binding), GO:0070273 (phosphatidylinositol-4-phosphate binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0000003 (reproduction), GO:0065007 (biological regulation), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0019953 (sexual reproduction), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0043934 (sporulation), GO:0009653 (anatomical structure morphogenesis), GO:0032505 (reproduction of a single-celled organism), GO:2000241 (regulation of reproductive process), GO:0051321 (meiotic cell cycle), GO:0019222 (regulation of metabolic process), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:0022413 (reproductive process in single-celled organism), GO:0071704 (organic substance metabolic process), GO:0003006 (developmental process involved in reproduction), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0009892 (negative regulation of metabolic process), GO:0044237 (cellular metabolic process), GO:2000242 (negative regulation of reproductive process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:1903046 (meiotic cell cycle process), GO:0048523 (negative regulation of cellular process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0045833 (negative regulation of lipid metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0019637 (organophosphate metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0030435 (sporulation resulting in formation of a cellular spore), GO:0031324 (negative regulation of cellular metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:0006793 (phosphorus metabolic process), GO:0034293 (sexual sporulation), GO:0010876 (lipid localization), GO:0051726 (regulation of cell cycle), GO:0030437 (ascospore formation), GO:0006629 (lipid metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0043935 (sexual sporulation resulting in formation of a cellular spore), GO:0010562 (positive regulation of phosphorus metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051055 (negative regulation of lipid biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0006644 (phospholipid metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0010468 (regulation of gene expression), GO:0046890 (regulation of lipid biosynthetic process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0046486 (glycerolipid metabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0051247 (positive regulation of protein metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0019216 (regulation of lipid metabolic process), GO:0051253 (negative regulation of RNA metabolic process), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0051174 (regulation of phosphorus metabolic process), GO:0042304 (regulation of fatty acid biosynthetic process), GO:0045937 (positive regulation of phosphate metabolic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0019220 (regulation of phosphate metabolic process), GO:0006650 (glycerophospholipid metabolic process), GO:1903725 (regulation of phospholipid metabolic process), GO:0006897 (endocytosis), GO:1902680 (positive regulation of RNA biosynthetic process), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0031401 (positive regulation of protein modification process), GO:0045936 (negative regulation of phosphate metabolic process), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0042325 (regulation of phosphorylation), GO:0045893 (positive regulation of DNA-templated transcription), GO:0045892 (negative regulation of DNA-templated transcription), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:0042327 (positive regulation of phosphorylation), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0001934 (positive regulation of protein phosphorylation), GO:0045944 (positive regulation of transcription by RNA polymerase II), GO:0000122 (negative regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0071944 (cell periphery), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0010008 (endosome membrane), GO:0098588 (bounding membrane of organelle), GO:0031982 (vesicle), GO:0030659 (cytoplasmic vesicle membrane), GO:0012506 (vesicle membrane), GO:0097708 (intracellular vesicle)