Protein: Acyl-coenzyme A diphosphatase fit1 (EC 3.6.1.-) (Acyl-coenzyme A diphosphatase scs3) (FIT family protein scs3) (Q9HGM4)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate (By similarity). Preferentially hydrolyzes unsaturated long-chain acyl-CoA substrates in the endoplasmic reticulum (ER) lumen (By similarity). This catalytic activity is required for maintaining ER structure and for lipid droplets (LDs) biogenesis, which are lipid storage organelles involved in maintaining lipid and energy homeostasis (By similarity). May directly bind to diacylglycerol (DAGs) and triacylglycerol, which is also important for LD biogenesis (By similarity). May support directional budding of nacent LDs from the ER into the cytosol by reducing DAG levels at sites of LD formation (By similarity). May play a role in the regulation of cell morphology and cytoskeletal organization (By similarity).
Subcellular location: Endoplasmic reticulum membrane {ECO:0000255|HAMAP-Rule:MF_03231, ECO:0000269|PubMed:16823372}; Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_03231}. Note=Enriched at sites of lipid droplet (LD) biogenesis. {ECO:0000250|UniProtKB:P53012}.
Sequence: MTEKTASHYWNEETSILKLRRKDILLFEIYATTLLLGSIYSIYVDKWSITSYFGNSKNLINLIFVKRGWFWTSLVYFYHAWDQKRNKIDFKFISRYIVATLWWMFVTQWFIGPGLIDRTFALSGGSCKNFDGDSSVFIPLTASTCKGLNGSWSGGHDLSGHVFLLTHSSLFMLSENFSFILNNGIKATSTKVLFGLLGLWWWMLFVTASFYHTTFEKCTGFFSGILEWSIVYVFSSRMPAVADLLGSSDY
InterPro domains: - IPR046400: Acyl-coenzyme A diphosphatase SCS3 (family) [16-235]
- IPR019388: Fat storage-inducing transmembrane protein (family) [20-237]
Interaction partners: - Probable diacylglycerol pyrophosphate phosphatase 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0022406 (membrane docking), GO:0051641 (cellular localization), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0051235 (maintenance of location), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0033036 (macromolecule localization), GO:0010876 (lipid localization), GO:0044085 (cellular component biogenesis), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0019915 (lipid storage), GO:0019637 (organophosphate metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0044249 (cellular biosynthetic process), GO:0006644 (phospholipid metabolic process), GO:0090407 (organophosphate biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0140042 (lipid droplet formation), GO:0006996 (organelle organization), GO:0008610 (lipid biosynthetic process), GO:0045017 (glycerolipid biosynthetic process), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0034389 (lipid droplet organization), GO:0008654 (phospholipid biosynthetic process), GO:0140694 (non-membrane-bounded organelle assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031984 (organelle subcompartment), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0031090 (organelle membrane), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0043231 (intracellular membrane-bounded organelle), GO:0005789 (endoplasmic reticulum membrane)