Protein: Riboflavin kinase (EC 2.7.1.26) (ATP:riboflavin 5'-phosphotransferase) (Flavokinase) (KOI-4) (Q8CFV9)
Organism: Mus musculus (Mouse)
Function: Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase (By similarity).
Subcellular location: Cytoplasm {ECO:0000250}.
Sequence: MRSLPFFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADVSTGIYYGWASVGSGDVHKMVVSIGWNPYYKNVKKSMETHIIHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKQLDLPEHLKLKDDNFFQVSKGKIMNGH
InterPro domains: - IPR023468: Riboflavin kinase (family) [2-148]
- IPR023465: Riboflavin kinase domain superfamily (homologous_superfamily) [2-148]
- IPR015865: Riboflavin kinase domain, bacterial/eukaryotic (domain) [1-131]
Interaction partners: - FAD synthase
- Tartrate-resistant acid phosphatase type 5
- Flavin reductase
- Lysosomal acid phosphatase
- Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
- Tumor necrosis factor receptor superfamily member 1A
- Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
- NADPH oxidase 1
- Tumor necrosis factor receptor type 1-associated DEATH domain protein
- Cytochrome b-245 light chain
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0019637 (organophosphate metabolic process), GO:0005996 (monosaccharide metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0005975 (carbohydrate metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0006793 (phosphorus metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0019318 (hexose metabolic process), GO:0046364 (monosaccharide biosynthetic process), GO:0019693 (ribose phosphate metabolic process), GO:0016051 (carbohydrate biosynthetic process), GO:0005976 (polysaccharide metabolic process), GO:0034637 (cellular carbohydrate biosynthetic process), GO:0090407 (organophosphate biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0072527 (pyrimidine-containing compound metabolic process), GO:0044264 (cellular polysaccharide metabolic process), GO:0006006 (glucose metabolic process), GO:0009259 (ribonucleotide metabolic process), GO:0006006 (glucose metabolic process), GO:0009259 (ribonucleotide metabolic process), GO:0006006 (glucose metabolic process), GO:0006220 (pyrimidine nucleotide metabolic process), GO:0009117 (nucleotide metabolic process), GO:0033692 (cellular polysaccharide biosynthetic process), GO:0044042 (glucan metabolic process), GO:0019319 (hexose biosynthetic process), GO:0000271 (polysaccharide biosynthetic process), GO:0046474 (glycerophospholipid biosynthetic process), GO:0006094 (gluconeogenesis), GO:0033036 (macromolecule localization), GO:0006281 (DNA repair), GO:0006073 (cellular glucan metabolic process), GO:0009250 (glucan biosynthetic process), GO:0009165 (nucleotide biosynthetic process), GO:0006221 (pyrimidine nucleotide biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)