Protein: Tudor and KH domain-containing protein (Tudor domain-containing protein 2) (Q80VL1)
Organism: Mus musculus (Mouse)
Function: Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.
Subcellular location: Cytoplasm. Mitochondrion. Note=Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Colocalizes with pi- and piP-bodies, a subset of the nuage which contains secondary piRNAs. Associated with mitochondria in the germline.
Sequence: MSTERTSWTNLSTIQKIALGLGIPASATVAYILYRRYRESREERLTFVGEDDIEIEMRVPQEAVKLIIGRQGANIKQLRKQTGARIDVDTEDVGDERVLLISGFPVQVCKAKAAIHQILTENTPVFEQLSVPQRSVGRIIGRGGETIRSICKASGAKITCDKESEGTLLLSRLIKISGTQKEVAAAKHLILEKVSEDEELRKRIAHSAETRVPRKQPISVRREEVTEPGGAGEAALWKNTNSSMGPATPLEVPLRKGGGDMVVVGPKEGSWEKPNDDSFQNSGAQSSPETSMFEIPSPDFSFHADEYLEVYVSASEHPNHFWIQIIGSRSLQLDKLVSEMTQHYENSLPEDLTVHVGDIVAAPLSTNGSWYRARVLGTLENGNLDLYFVDFGDNGDCALKDLRALRSDFLSLPFQAIECSLARIAPTGEEWEEEALDEFDRLTHCADWKPLVAKISSYVQTGISTWPKIYLYDTSDEKKLDIGLELVRKGYAVELPEDMEENRTVPNMLKDMATETDDSLASILTETKKSPEEMPHTLSCLSLSEAASMSGDDNLEDDLF
InterPro domains: - IPR035437: SNase-like, OB-fold superfamily (homologous_superfamily) [310-492]
- IPR050621: Tudor domain-containing (family) [10-512]
- IPR036612: K Homology domain, type 1 superfamily (homologous_superfamily) [48-206]
- IPR047382: Tudor and KH domain-containing protein, first type I K homology domain (domain) [49-119]
- IPR004087: K Homology domain (domain) [51-195]
- IPR002999: Tudor domain (domain) [303-422]
- IPR047380: Tudor domain-containing protein 2-like, tudor domain (domain) [328-420]
- IPR047381: Tudor and KH domain-containing protein, second type I K homology domain (domain) [124-205]
- IPR004088: K Homology domain, type 1 (domain) [55-192]
Interaction partners: - Piwi-like protein 1
- Piwi-like protein 4
- Protein maelstrom homolog
- ATP-dependent RNA helicase TDRD9
- Tudor domain-containing protein 6
- Piwi-like protein 2
- Poly
- Small RNA 2'-O-methyltransferase
- Inactive peptidyl-prolyl cis-trans isomerase FKBP6
- Mitochondrial cardiolipin hydrolase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044238 (primary metabolic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0032259 (methylation), GO:0006807 (nitrogen compound metabolic process), GO:0043414 (macromolecule methylation), GO:1901564 (organonitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0019538 (protein metabolic process), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0006479 (protein methylation), GO:0008213 (protein alkylation), GO:0018022 (peptidyl-lysine methylation), GO:0016571 (histone methylation), GO:0018193 (peptidyl-amino acid modification), GO:0016570 (histone modification), GO:0018205 (peptidyl-lysine modification), GO:0034968 (histone lysine methylation), GO:0051568 (histone H3-K4 methylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)