Protein: Elongator complex protein 6 (Q9VFW4)
Organism: Drosophila melanogaster (Fruit fly)
Function: Component of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. Binding by the elongator complex stabilizes microtubules and promotes their growth. This induces central spindle asymmetry, promoting polarized signaling endosome trafficking during asymmetric cell division and cell fate assignation of sensory organ precursor cells. Required in germ line cells for microtubule organization involved in oocyte polarization and chromosome organization. Involved in InR-TOR (insulin-like receptor-target of rapamycin) signaling regulation of cellular metabolism, autophagy and apoptosis
Subcellular location: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Concentrates in the nucleus upon insulin stimulation. During asymmetric cell division of sensory organ precursor cells the elongator complex preferentially binds and stabilizes microtubules on the anterior side (pIIb daughter cell) of the spindle
Sequence: MATSVLLACGLNEQKLPGFVHISEESNVDASFLISCVLGQRLRISNAGTLLVCLQHHYQHYFNAGMRLGYNTNIFQGKTLGVIDVLSDMAGEGLASKWLTNTEGQTLTDQLVEDIRAQVERNYASRNSYTVLIDNLSILFNLGASKLQVQQFCQDLAALGKEREKLTVITKLSNSDIYQLTDNNVAKLGQVRIQVLRLKSGVFREVDGKLLIERVLDEGNYACEETRKEVLYKVNDRNVKVFAPGEIGVKV
InterPro domains: - IPR018627: Elongator complex protein 6 (family) [6-246]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [3-243]
Interaction partners: - Elongator complex protein 5
- Elongator complex protein 4
- Elongator complex protein 3
- Elongator complex protein 1
- TAR DNA-binding protein 43
- Tetraspanin 68C, isoform A
- Elongator complex protein 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0044877 (protein-containing complex binding), GO:0005515 (protein binding), GO:0005102 (signaling receptor binding), GO:0005158 (insulin receptor binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0022414 (reproductive process), GO:0002376 (immune system process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0009605 (response to external stimulus), GO:0019953 (sexual reproduction), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0023056 (positive regulation of signaling), GO:0007275 (multicellular organism development), GO:0007163 (establishment or maintenance of cell polarity), GO:0048584 (positive regulation of response to stimulus), GO:0042440 (pigment metabolic process), GO:0006955 (immune response), GO:0007389 (pattern specification process), GO:0071840 (cellular component organization or biogenesis), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0032504 (multicellular organism reproduction), GO:0003006 (developmental process involved in reproduction), GO:0044237 (cellular metabolic process), GO:0022412 (cellular process involved in reproduction in multicellular organism), GO:0009607 (response to biotic stimulus), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0002252 (immune effector process), GO:0023051 (regulation of signaling), GO:0021700 (developmental maturation), GO:0006950 (response to stress), GO:0007017 (microtubule-based process), GO:0048609 (multicellular organismal reproductive process), GO:0051707 (response to other organism), GO:0048522 (positive regulation of cellular process), GO:0007276 (gamete generation), GO:1901615 (organic hydroxy compound metabolic process), GO:0009967 (positive regulation of signal transduction), GO:0007281 (germ cell development), GO:0007309 (oocyte axis specification), GO:0009798 (axis specification), GO:0043207 (response to external biotic stimulus), GO:0048468 (cell development), GO:1900078 (positive regulation of cellular response to insulin stimulus), GO:0030154 (cell differentiation), GO:0006952 (defense response), GO:0009966 (regulation of signal transduction), GO:0007308 (oocyte construction), GO:0000226 (microtubule cytoskeleton organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0035010 (encapsulation of foreign target), GO:0045087 (innate immune response), GO:0098542 (defense response to other organism), GO:0071695 (anatomical structure maturation), GO:1900076 (regulation of cellular response to insulin stimulus), GO:1901360 (organic cyclic compound metabolic process), GO:0008103 (oocyte microtubule cytoskeleton polarization), GO:0010646 (regulation of cell communication), GO:0048469 (cell maturation), GO:0016043 (cellular component organization), GO:0030952 (establishment or maintenance of cytoskeleton polarity), GO:0010647 (positive regulation of cell communication), GO:0009994 (oocyte differentiation), GO:0006582 (melanin metabolic process), GO:0016325 (oocyte microtubule cytoskeleton organization), GO:0046626 (regulation of insulin receptor signaling pathway), GO:0030951 (establishment or maintenance of microtubule cytoskeleton polarity), GO:0048477 (oogenesis), GO:0046628 (positive regulation of insulin receptor signaling pathway), GO:0018958 (phenol-containing compound metabolic process), GO:0035006 (melanization defense response), GO:0007292 (female gamete generation), GO:0048599 (oocyte development), GO:0035011 (melanotic encapsulation of foreign target), GO:0006996 (organelle organization), GO:0007010 (cytoskeleton organization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex)