Protein: 3-hydroxykynurenine transaminase (EC 2.6.1.63) (3-hydroxykynurenine transaminase and alanine--glyoxylate aminotransferase) (Ae-HKT/AGT) (Alanine--glyoxylate aminotransferase) (EC 2.6.1.44) (Q0IG34)
Organism: Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Function: Catalyzes the pyridoxal 5'-phosphate-dependent transamination of both 3-hydroxykynurenine and L-kynurenine to xanthurenic acid and kynurenic acid, respectively, preferentially using the alpha-ketoacid pyruvate, glyoxylate or oxaloacetate as the amino group acceptor. The affinity and catalytic efficiency for 3-hydroxykynurenine is higher than for L-kynurenine. Involved in the detoxification of cytotoxic metabolite 3-hydroxykynurenine generated by the hydroxylation of L-kynurenine, an intermediate in the tryptophan catabolism pathway. Also catalyzes, although with a lesser efficiency, the transamination of alanine with glyoxylate as an amino group acceptor. May play a role in the detoxification of glyoxylate, a toxic plant metabolite from the diet (Probable).
Subcellular location: Peroxisome {ECO:0000250|UniProtKB:P21549}.
Sequence: MKFTPPPSSLRGPLVIPDKIMMGPGPSNCSKRVLAALNNTCLSNFHDELFQVIDEVKDGLRYIFQTENRTTMCITGSAHTGMEALLCNLLEEGDIVLIANNGIWAERAINMATRYGADVRVLGGPADKPFSMTDFKKAIEQHRPKCLFVVHGDSSSGLLQPLEGLGKICHDYDCLLLVDAVASLCGVPFYMDKWEIDGVYTGSQKVLGAPPGITPISISPKALEVIRSRKTPSKVFYWDLLILGNYWGCYDEQKRYHHTVPSNLIFALREAIAQIAEEGLEPVIRRRQECAEQMYRGLQAMGLEIFVKDPEYRLPTVTCIMIPKGVNWWKVSEYAMNNFSLEIQGGFGPTMGIAWRAGIMGESSTLQRVNFYLYAFKESLKATHPDYVFEKKNGQTNGTK
InterPro domains: - IPR024169: Serine-pyruvate aminotransferase/2-aminoethylphosphonate-pyruvate transaminase (family) [14-399]
- IPR015421: Pyridoxal phosphate-dependent transferase, major domain (homologous_superfamily) [30-278]
- IPR015424: Pyridoxal phosphate-dependent transferase (homologous_superfamily) [13-382]
- IPR015422: Pyridoxal phosphate-dependent transferase, small domain (homologous_superfamily) [16-377]
- IPR000192: Aminotransferase class V domain (domain) [41-379]
Interaction partners: - malate synthase
- Serine hydroxymethyltransferase
- AAEL005819-PA
- Phosphoserine phosphatase
- Glyoxylate reductase/hydroxypyruvate reductase
- Glyoxylate reductase/hydroxypyruvate reductase
- Glyoxylate reductase/hydroxypyruvate reductase
- Aminomethyltransferase
- (S)-2-hydroxy-acid oxidase
- (S)-2-hydroxy-acid oxidase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0016740 (transferase activity), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0019842 (vitamin binding), GO:0043168 (anion binding), GO:0016769 (transferase activity, transferring nitrogenous groups), GO:0070279 (vitamin B6 binding), GO:0008483 (transaminase activity), GO:0030170 (pyridoxal phosphate binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0009056 (catabolic process), GO:0044282 (small molecule catabolic process), GO:1901575 (organic substance catabolic process), GO:0006081 (cellular aldehyde metabolic process), GO:0044248 (cellular catabolic process), GO:0006082 (organic acid metabolic process), GO:0016054 (organic acid catabolic process), GO:0046185 (aldehyde catabolic process), GO:0043436 (oxoacid metabolic process), GO:0046487 (glyoxylate metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0046395 (carboxylic acid catabolic process), GO:0072329 (monocarboxylic acid catabolic process), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)