Protein: DNA mismatch endonuclease Vsr (EC 3.1.-.-) (Type II nicking enzyme) (V.EcoKDcm) (Very short patch repair protein) (Vsr mismatch endonuclease) (P09184)
Organism: Escherichia coli (strain K12)
Function: Deamination of 5-methylcytosine in DNA results in T/G mismatches. If unrepaired, these mismatches can lead to C-to-T transition mutations. The very short patch (VSP) repair process in E.coli counteracts the mutagenic process by repairing the mismatches in favor of the G-containing strand. This enzyme is an endonuclease that nicks double-stranded DNA within the sequence CT(AT)GN or NT(AT)GG next to the thymidine residue that is mismatched to 2'-deoxyguanosine. The incision is mismatch-dependent and strand-specific.
Sequence: MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDASLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDISRLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA
InterPro domains: - IPR004603: DNA mismatch endonuclease VSR (family) [1-150]
- IPR011335: Restriction endonuclease type II-like (homologous_superfamily) [2-153]
Interaction partners: - DNA-cytosine methyltransferase
- DNA mismatch repair protein MutL
- DNA mismatch repair protein MutH
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0140098 (catalytic activity, acting on RNA), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0004518 (nuclease activity), GO:0004540 (ribonuclease activity), GO:0004519 (endonuclease activity), GO:0004521 (RNA endonuclease activity), GO:0016891 (RNA endonuclease activity, producing 5'-phosphomonoesters), GO:0016893 (endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters), GO:0004523 (RNA-DNA hybrid ribonuclease activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0010467 (gene expression), GO:0042254 (ribosome biogenesis), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0034470 (ncRNA processing), GO:0000966 (RNA 5'-end processing), GO:0006364 (rRNA processing), GO:0034660 (ncRNA metabolic process), GO:0016072 (rRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:1990904 (ribonucleoprotein complex), GO:1902494 (catalytic complex), GO:0140535 (intracellular protein-containing complex), GO:1905348 (endonuclease complex), GO:1905347 (endodeoxyribonuclease complex)