Protein: Na(+)/H(+) exchange regulatory cofactor NHE-RF4 (NHERF-4) (Intestinal and kidney-enriched PDZ protein) (Natrium-phosphate cotransporter IIa C-terminal-associated protein 2) (Na/Pi cotransporter C-terminal-associated protein 2) (NaPi-Cap2) (PDZ domain-containing protein 2) (PDZ domain-containing protein 3) (Sodium-hydrogen exchanger regulatory factor 4) (Q86UT5)
Organism: Homo sapiens (Human)
Function: Acts as a regulatory protein that associates with GUCY2C and negatively modulates its heat-stable enterotoxin-mediated activation. Stimulates SLC9A3 activity in the presence of elevated calcium ions.
Subcellular location: Cell membrane {ECO:0000269|PubMed:11950846, ECO:0000269|PubMed:19088451, ECO:0000269|PubMed:22627094}; Peripheral membrane protein {ECO:0000269|PubMed:11950846, ECO:0000269|PubMed:19088451}. Cytoplasm {ECO:0000269|PubMed:19088451}. Note=Preferentially accumulates at the apical surface and ileal brush border of intestinal epithelial cells (PubMed:11950846, PubMed:19088451). {ECO:0000269|PubMed:11950846, ECO:0000269|PubMed:19088451}.
Sequence: MVTPSPPGNHSLSLEAPRLHTASDLLGNHSLGLPLITALVGSRDRRGRVFSPVPVPLPTNPTTQHPTRQKLPSTLSGHRVCQAHGEPVLGLCPLLPLFCCPPHPPDPWSLERPRFCLLSKEEGKSFGFHLQQELGRAGHVVCRVDPGTSAQRQGLQEGDRILAVNNDVVEHEDYAVVVRRIRASSPRVLLTVLARHAHDVARAQLGEDAHLCPTLGPGVRPRLCHIVKDEGGFGFSVTHGNQGPFWLVLSTGGAAERAGVPPGARLLEVNGVSVEKFTHNQLTRKLWQSGQQVTLLVAGPEVEEQCRQLGLPLAAPLAEGWALPTKPRCLHLEKGPQGFGFLLREEKGLDGRPGQFLWEVDPGLPAKKAGMQAGDRLVAVAGESVEGLGHEETVSRIQGQGSCVSLTVVDPEADRFFSMVRLSPLLFLENTEAPASPRGSSSASLVETEDPSLEDTSVPSVPLGSRQCFLYPGPGGSYGFRLSCVASGPRLFISQVTPGGSAARAGLQVGDVILEVNGYPVGGQNDLERLQQLPEAEPPLCLKLAARSLRGLEAWIPPGAAEDWALASDLL
InterPro domains: - IPR036034: PDZ superfamily (homologous_superfamily) [101-554]
- IPR001478: PDZ domain (domain) [115-548]
- IPR051067: Na+/H+ exchange regulatory cofactor (family) [108-564]
Interaction partners: - Guanylyl cyclase C
- Cystic fibrosis transmembrane conductance regulator
- Sodium-dependent phosphate transport protein 2A
- Atrial natriuretic peptide receptor 3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0051234 (establishment of localization), GO:0050793 (regulation of developmental process), GO:0071840 (cellular component organization or biogenesis), GO:0065008 (regulation of biological quality), GO:0051641 (cellular localization), GO:0007017 (microtubule-based process), GO:0033036 (macromolecule localization), GO:0050794 (regulation of cellular process), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0000226 (microtubule cytoskeleton organization), GO:0031647 (regulation of protein stability), GO:0006810 (transport), GO:0022603 (regulation of anatomical structure morphogenesis), GO:0022604 (regulation of cell morphogenesis), GO:0050821 (protein stabilization), GO:0008104 (protein localization), GO:0006996 (organelle organization), GO:0008360 (regulation of cell shape), GO:0007010 (cytoskeleton organization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus)