Protein: EKC/KEOPS complex subunit SPAP27G11.07c (EC 3.6.-.-) (Atypical serine/threonine protein kinase BUD32 homolog) (EC 2.7.11.1) (Q9P7N1)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. BUD32 has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit KAE1. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators.
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:P53323}. Nucleus {ECO:0000250|UniProtKB:P53323}. Chromosome, telomere {ECO:0000250|UniProtKB:P53323}.
Sequence: MSEKPDLRQRCSDIYREIKEKKLTVVKQGAEAITIKTEFYPGEVCLLKCRPAKRWRHPILDQKLSRKRCLVEARLLAKCHYVGIKCPMLYFIDANRGQIYMEWIDGPCVRDYIREICECEIEKKLIPLMKRIGSEVAKMHKNDIVHGDLTTSNMMLESHNNPVPIFIDFGLGSVSESEEDKAVDIYVLERALSSTLPESESLFHHVLDSYAQSWKQSKATLRRFEEVRMRGRKRTMIG
InterPro domains: - IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [23-186]
- IPR008266: Tyrosine-protein kinase, active site (active_site) [144-156]
- IPR000719: Protein kinase domain (domain) [20-238]
- IPR022495: Serine/threonine-protein kinase Bud32 (family) [29-232]
Interaction partners: - tRNA N6-adenosine threonylcarbamoyltransferase
- EKC/KEOPS complex subunit cgi121
- EKC/KEOPS complex subunit SPAC4H3.13
- Threonylcarbamoyl-AMP synthase
- tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial
- 18S rRNA aminocarboxypropyltransferase
- EKC/KEOPS complex subunit gon7
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0050793 (regulation of developmental process), GO:2000243 (positive regulation of reproductive process), GO:2000241 (regulation of reproductive process), GO:0048584 (positive regulation of response to stimulus), GO:0051240 (positive regulation of multicellular organismal process), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0051239 (regulation of multicellular organismal process), GO:0051094 (positive regulation of developmental process), GO:0048522 (positive regulation of cellular process), GO:0051726 (regulation of cell cycle), GO:0009967 (positive regulation of signal transduction), GO:0048580 (regulation of post-embryonic development), GO:2000026 (regulation of multicellular organismal development), GO:0051445 (regulation of meiotic cell cycle), GO:0010646 (regulation of cell communication), GO:0080134 (regulation of response to stress), GO:0080135 (regulation of cellular response to stress), GO:0009966 (regulation of signal transduction), GO:0048582 (positive regulation of post-embryonic development), GO:0051446 (positive regulation of meiotic cell cycle), GO:0032886 (regulation of microtubule-based process), GO:0051128 (regulation of cellular component organization), GO:0010564 (regulation of cell cycle process), GO:0044087 (regulation of cellular component biogenesis), GO:1902903 (regulation of supramolecular fiber organization), GO:0070507 (regulation of microtubule cytoskeleton organization), GO:0043254 (regulation of protein-containing complex assembly), GO:0090224 (regulation of spindle organization), GO:0062197 (cellular response to chemical stress), GO:0060284 (regulation of cell development), GO:0010720 (positive regulation of cell development), GO:0033043 (regulation of organelle organization), GO:1902533 (positive regulation of intracellular signal transduction), GO:0050769 (positive regulation of neurogenesis), GO:0051960 (regulation of nervous system development), GO:1902531 (regulation of intracellular signal transduction), GO:0043408 (regulation of MAPK cascade), GO:0051493 (regulation of cytoskeleton organization), GO:0031110 (regulation of microtubule polymerization or depolymerization), GO:0032271 (regulation of protein polymerization), GO:0050767 (regulation of neurogenesis), GO:0031113 (regulation of microtubule polymerization), GO:0032872 (regulation of stress-activated MAPK cascade), GO:0051495 (positive regulation of cytoskeleton organization), GO:1901673 (regulation of mitotic spindle assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)