Protein: Nuclear protein localization protein 4 homolog 1 (H2KYU6)
Organism: Caenorhabditis elegans
Function: In association with ufd-1 and ATPase cdc-48.1 and/or cdc-48.2, involved in the cytoplasmic elimination of misfolded proteins exported from the ER. This pathway, known as ERAD, prevents the activation of the unfolded protein response (UPR) caused by the accumulation of misfolded proteins in the ER. During S phase and in association with ufd-1, cdc-48.1 and/or cdc-48.2 and ubxn-3, ensures the degradation of DNA licensing factor cdt-1 after the initiation of DNA replication and thus the disassembly of the DNA replication CGM helicase complex by promoting the dissociation from chromatin of several of its components including cdc-45 and sld-5. Regulates ubxn-3 nuclear localization during S phase.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:18728180}. Nucleus {ECO:0000269|PubMed:18728180}. Note=Localizes to the cytoplasm during mitosis. Nuclear localization upon nuclear membrane re-assembly is cdc-48-dependent. {ECO:0000269|PubMed:18728180}.
Sequence: MVLEVPQTERVNDVDVFLSTQDGQIQRPKGPNCRHPVRQKCTNCLPVDPFDEEYLKEKDIKHMSFHAHVRKLLGSQGKGTTLKKPLENFRCSLKPNCDAHKPFPKGICTKCKPQVVTLNRQKFRHVDNIQIENQELVNQFLDYWRLSGHQRVGFLIGQYQPHLEVPLGIKATVAAIYEPPQHCREDGIEFLEDKNQKTIDNLLEMLGLQRVGWIFTDCWTANSAEGTVHYTRHKDSFFLSAEECITAAMLQNQHPNITEYSMDRHYGSKFVTVVASGDESMHVNFHGYQVSNQCAAMVEADILCPTLYTPELAYVRETPLSEEHYITDVQFSMKNEYGAEVMKNGRPLPVEYLLVDVPAGMPKEPHYTFHVGTSNKSKSAKFNVENRQAIGQLQGGANLIQYSSEFSKNQFLEQATNFHFLLYLVTNDQVQISDEWMKRLCDAVKAQDRGTAMEWAQECEDWHQLMALAHANGGSGNDVSDIPVIPNGDPFSGSSSGGSGGAVWNCGHCTFQNEAARQDCSMCGLPAAD
InterPro domains: - IPR016563: Nuclear protein localization protein 4 (family) [3-517]
- IPR037518: MPN domain (domain) [129-266]
- IPR036443: Zinc finger, RanBP2-type superfamily (homologous_superfamily) [500-526]
- IPR007717: Nuclear pore localisation protein NPL4, C-terminal (domain) [117-468]
- IPR007716: NPL4, zinc-binding putative (domain) [12-148]
- IPR001876: Zinc finger, RanBP2-type (domain) [499-529]
Interaction partners: - Ubiquitin fusion degradation protein 1 homolog
- Transitional endoplasmic reticulum ATPase homolog 1
- Transitional endoplasmic reticulum ATPase homolog 2
- Nuclear protein localization protein 4 homolog 2
- ATPase family protein 2 homolog
- Ubiquitin fusion degradation protein 3 homolog
- Ubiquitin conjugation factor E4 ufd-2
- Derlin-2
- Derlin-1
- Ubiquitin-ribosomal protein eL40 fusion protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0042221 (response to chemical), GO:0006807 (nitrogen compound metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901698 (response to nitrogen compound), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0019538 (protein metabolic process), GO:0035966 (response to topologically incorrect protein), GO:0009790 (embryo development), GO:1901575 (organic substance catabolic process), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0034976 (response to endoplasmic reticulum stress), GO:0009057 (macromolecule catabolic process), GO:0035967 (cellular response to topologically incorrect protein), GO:0010243 (response to organonitrogen compound), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0051788 (response to misfolded protein), GO:0071310 (cellular response to organic substance), GO:0030163 (protein catabolic process), GO:0009792 (embryo development ending in birth or egg hatching), GO:0071218 (cellular response to misfolded protein), GO:0036503 (ERAD pathway), GO:0010498 (proteasomal protein catabolic process), GO:0051603 (proteolysis involved in protein catabolic process), GO:0071712 (ER-associated misfolded protein catabolic process), GO:0006515 (protein quality control for misfolded or incompletely synthesized proteins)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140534 (endoplasmic reticulum protein-containing complex), GO:0016020 (membrane), GO:0031984 (organelle subcompartment), GO:0005737 (cytoplasm), GO:0098796 (membrane protein complex), GO:0012505 (endomembrane system), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0031090 (organelle membrane), GO:0034098 (VCP-NPL4-UFD1 AAA ATPase complex), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0043231 (intracellular membrane-bounded organelle), GO:0005789 (endoplasmic reticulum membrane), GO:0005634 (nucleus)