Protein: Linoleate 10R-lipoxygenase (EC 1.13.11.62) (Cyclooxygenase-like fatty acid oxygenase) (Fatty acid oxygenase ppoC) (Linoleate 10R-dioxygenase) (10R-DOX) (Psi-producing oxygenase C) (AfPpoC) (Q4WY82)
Organism: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata)
Function: Responsible for the synthesis of various fatty acid-derived oxylipins. Oxidizes linoleic acid primarily to 10R-hydroperoxy-8,12-octadecadienoic acid (10R-HPODE) and, to a lesser extent, 8R-hydroperoxylinoleic acid (8R-HPODE). Also synthesizes 10-hydroxy-octadeca-8,12-dienoic acid (10-HODE) from linoleic acid and primarily 8R-hydroxy-octadeca-9-monoenoic acid (8-HOME, also known as psiB beta) from oleic acid. 8-HOME forms part of psi factor, a mixture of oxylipins that regulates the balance between sexual and asexual spore production. Displays epoxyalcohol synthase activity. Plays a role in the synthesis of prostaglandins which may be required for pathogenicity.
Sequence: MLRRFSSTFKKKGDRESKQNGTASSSSAAVANTNNNDNKRHSKISAARKSSSDDDRNEKKGNSVSPFEKYASVLHASRSPIPNQTGDGAYLEHEHTTSLLQDARHLGFKDFKTLKEVIESKLPGGQLIDDKTMLMERIIQLVSRLPHNSKHREELTNAFLTELWDSLPHPPLSYMGNDYAYRSADGSNNNPTLPRLGAANTLYARTIPPLIIQPGGLPDPGLVFDTLFARQTFKPHPNKVSSVFFYWASLIIHDIFQTDYKNPNMNKTSGYLDLSILYGDVQEEQNLIRTFKDGKLKPDSFSEPRLQAFPATCCVLMVMLNRFHNYAVEQLAAINENGRFTKPADNLSEEEAKKAWAKYDEDLFQTGRLITCGLYINITLYDYLRTIVNLNRTNSTWCLDPRAQMEGSHTAPSGLGNQCSVEFNLAYRWHSATSATDEKWTEDVYERLMGKPASEVSMTELLMGLGKYQAELPKDPSKRTFADLERQADGRFKDEDLVNLLVNAVEDVAGSFGARNVPKVLKNVEILGIIQSRKWNVGSLNEFRKFFGLKPYETFEEINSDPDVAESLRSLYDHPDFVELYPGIVAEEAKQPMVPGVGIAPTYTISRAVLSDAVALVRGDRFYTIDYNPRNLTNWGYSEVRYDLSINQGCIFYKLATRAFPNWFKPDSIYAHYPMTIPSENRKIMKDLGREIHYSWDRPQYTPPRVDLVSYSNAKLVAEQQNQFRAAWGDTVEFVFGKASKEFKLYQDSAFIQKHADVMSKLLNKEEWHRSVKEFYEDITAKLLEDKTRRFGGINQVDITNDVGNLTPVIFAANVFSLPLKSKENPRGIYTEHEMFKVLAALYNCLYFDIDKTKSYPLHHASQAVGEPLGKALEANVKALGGSSLLSGIFRSFRENKNALKEYGVHLTKQLLENGLGAHEIAWAQFLPTVIAMVPAQAQAFTQIVDFYLSKEGSKHLPAIQRLAKQDTKKSDEQLLHYCLEAVRLNDMSGLYRQSETTLAVTDEAVEVTIQPGDKVFVSFAKANRDASVFPDPAEVRLDRPMNSYINPTLGPHGFLSKETSHIALTAMLRAVGRLNNLRVAPGVQGQLKKIPQPGGYSAYLREDHGSYSIFPTTFRVQYDA
InterPro domains: - IPR034812: Psi-factor producing oxygenase-like, N-terminal heme peroxidase domain (domain) [149-699]
- IPR036396: Cytochrome P450 superfamily (homologous_superfamily) [673-1091]
- IPR010255: Haem peroxidase superfamily (homologous_superfamily) [126-641]
- IPR050783: Oxylipin biosynthesis and metabolism (family) [61-993]
- IPR019791: Haem peroxidase, animal-type (family) [170-689]
- IPR037120: Haem peroxidase domain superfamily, animal type (homologous_superfamily) [126-677]
Interaction partners: - Psi-producing oxygenase A
- Fatty acid oxygenase, putative
- Lysophospholipase
- Patatin-like phospholipase domain-containing protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0051213 (dioxygenase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006631 (fatty acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:0016053 (organic acid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0006633 (fatty acid biosynthetic process), GO:0019752 (carboxylic acid metabolic process), GO:0033559 (unsaturated fatty acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0072330 (monocarboxylic acid biosynthetic process), GO:0032787 (monocarboxylic acid metabolic process), GO:0006690 (icosanoid metabolic process), GO:0046456 (icosanoid biosynthetic process), GO:0006636 (unsaturated fatty acid biosynthetic process), GO:0006692 (prostanoid metabolic process), GO:0006693 (prostaglandin metabolic process), GO:0046457 (prostanoid biosynthetic process), GO:0001516 (prostaglandin biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)