Protein: Threonylcarbamoyladenosine tRNA methylthiotransferase (EC 2.8.4.5) (tRNA-t(6)A37 methylthiotransferase) (Q584Z1)
Organism: Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Function: Not known
Sequence: MKRNSAREGDIEDFFCNSSLSAPRGNCAQNVNSRRRRVVREDYGPGIPGNATIFVHTFGCGHNVSDGEYMAGQLVESGYNVTDEFGQADAYLLNSCTVKNPSEEHFVSMMNRVRDTGKPLIVAGCVPQADPTNKQWGDVSVVGVRSIDCVSYVVQEALQGNCVRLLGETEDQRQSNESNELPALDLPKVRRNKYIEIIPISVGCLNNCTYCKTKQARGDLRSYPVEVIVDRVREVVRDGVKEIRLTSEDSGAYGIDIGTDVVYLLRAVAVELEGTDVMLRVGMSNPPYLLRHVDGFATVLKHPNVYEFVHIPVQSGSDSILQTMLREYTVEEFFMCIDSIRAAVPKATVATDIICAFPGEGESEWQETMELCKRAKFEVINITRFYPRRNTPAAAMKQIPTDVAKHRTTELTNFFNSYRTFDSMVGEVHNVTLLETAHDKHHLVGHTKNYVQVLVDPAQARMGESVVVVITSATKYSVMGRVLRSRWERLSADAVGFATAPFRTRKGRIASALLMATAVGTVLLYWSSTRRRRCK
InterPro domains: - IPR007197: Radical SAM (domain) [158-422]
- IPR013848: Methylthiotransferase, N-terminal (domain) [51-159]
- IPR002792: TRAM domain (domain) [422-484]
- IPR038135: Methylthiotransferase, N-terminal domain superfamily (homologous_superfamily) [52-178]
- IPR006638: Elp3/MiaA/NifB-like, radical SAM core domain (domain) [194-413]
- IPR020612: Methylthiotransferase, conserved site (conserved_site) [198-218]
- IPR005839: Methylthiotransferase (family) [52-480]
- IPR006466: MiaB-like tRNA modifying enzyme, archaea/eukaryota (family) [53-482]
- IPR023404: Radical SAM, alpha/beta horseshoe (homologous_superfamily) [192-422]
Interaction partners: - Exosome complex exonuclease RRP40
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016782 (transferase activity, transferring sulphur-containing groups)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0006412 (translation), GO:0043412 (macromolecule modification), GO:0006518 (peptide metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0043043 (peptide biosynthetic process), GO:0140053 (mitochondrial gene expression), GO:0032543 (mitochondrial translation), GO:0006396 (RNA processing), GO:0009451 (RNA modification), GO:1900864 (mitochondrial RNA modification), GO:0000963 (mitochondrial RNA processing), GO:0016071 (mRNA metabolic process), GO:0000959 (mitochondrial RNA metabolic process), GO:0006400 (tRNA modification), GO:2001056 (positive regulation of cysteine-type endopeptidase activity), GO:0043281 (regulation of cysteine-type endopeptidase activity involved in apoptotic process), GO:0043280 (positive regulation of cysteine-type endopeptidase activity involved in apoptotic process), GO:0006919 (activation of cysteine-type endopeptidase activity involved in apoptotic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0031090 (organelle membrane), GO:0031967 (organelle envelope), GO:0005739 (mitochondrion), GO:0043229 (intracellular organelle), GO:0019867 (outer membrane), GO:0043227 (membrane-bounded organelle), GO:0005740 (mitochondrial envelope), GO:0031968 (organelle outer membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0031966 (mitochondrial membrane), GO:0005741 (mitochondrial outer membrane)