Protein: Esterase LipC (EC 3.1.1.-) (P96402)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Esterase that can hydrolyze short-chain esters with the carbon chain containing 2 to 10 carbon atoms. Does not have lipase activity. Is highly immunogenic and elicits strong humoral immune responses in both HIV-negative (HIV-) and HIV-positive (HIV+) tuberculosis (TB) patients. Also elicits pro-inflammatory cytokine and chemokine responses from macrophages and pulmonary epithelial cells. May participate in the progression of active tuberculosis both by contributing to the utilization of lipid substrates for bacterial growth and replication, and by modulating immune responses.
Subcellular location: Cell surface {ECO:0000269|PubMed:22038913}. Secreted, cell wall {ECO:0000269|PubMed:22038913}. Secreted, capsule {ECO:0000269|PubMed:22038913}. Note=Cell surface protein that is present in both the cell wall and the capsule. {ECO:0000269|PubMed:22038913}.
Sequence: MNQRRAAGSTGVAYIRWLLRARPADYMLALSVAGGSLPVVGKHLKPLGGVTAIGVWGARHASDFLSATAKDLLTPGINEVRRRDRASTQEVSVAALRGIVSPDDLAVEWPAPERTPPVCGALRHRRYVHRRRVLYGDDPAQLLDVWRRKDMPTKPAPVLIFVPGGAWVHGSRAIQGYAVLSRLAAQGWVCLSIDYRVAPHHRWPRHILDVKTAIAWARANVDKFGGDRNFIAVAGCSAGGHLSALAGLTANDPQYQAELPEGSDTSVDAVVGIYGRYDWEDRSTPERARFVDFLERVVVQRTIDRHPEVFRDASPIQRVTRNAPPFLVIHGSRDCVIPVEQARSFVERLRAVSRSQVGYLELPGAGHGFDLLDGARTGPTAHAIALFLNQVHRSRAQFAKEVI
InterPro domains: - IPR029058: Alpha/Beta hydrolase fold (homologous_superfamily) [114-379]
- IPR049492: BD-FAE-like domain (domain) [144-349]
- IPR019826: Carboxylesterase type B, active site (active_site) [224-239]
- IPR050300: GDXG lipolytic enzyme (family) [99-402]
Interaction partners: - Possible esterase LipW
- Putative diacyglycerol O-acyltransferase Rv0221
- Putative hydrolase LipZ
- Probable conserved transmembrane protein
- Uncharacterized MFS-type transporter Rv2994
- Esterase LipU
- Triacylglycerol lipase
- Carboxylesterase/phospholipase LipF
- Probable conserved transmembrane protein
- Enoyl-CoA hydratase EchA19
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0016298 (lipase activity), GO:0052689 (carboxylic ester hydrolase activity), GO:0004806 (triglyceride lipase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009987 (cellular process), GO:0009605 (response to external stimulus), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0009607 (response to biotic stimulus), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0044403 (biological process involved in symbiotic interaction), GO:0051707 (response to other organism), GO:0009056 (catabolic process), GO:0051701 (biological process involved in interaction with host), GO:0043207 (response to external biotic stimulus), GO:0052173 (response to defenses of other organism), GO:0006979 (response to oxidative stress), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0044248 (cellular catabolic process), GO:0075136 (response to host), GO:1901700 (response to oxygen-containing compound), GO:1901575 (organic substance catabolic process), GO:0010033 (response to organic substance), GO:0034599 (cellular response to oxidative stress), GO:0009057 (macromolecule catabolic process), GO:0052200 (response to host defenses), GO:0062197 (cellular response to chemical stress), GO:0042542 (response to hydrogen peroxide), GO:1901701 (cellular response to oxygen-containing compound), GO:0000302 (response to reactive oxygen species), GO:0052572 (response to host immune response), GO:0034614 (cellular response to reactive oxygen species), GO:0070301 (cellular response to hydrogen peroxide), GO:0052553 (modulation by symbiont of host immune response), GO:0052562 (suppression by symbiont of host immune response)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)