Protein: Large ribosomal subunit protein uL5 (60S ribosomal protein L11) (Q9CXW4)
Organism: Mus musculus (Mouse)
Function: Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53. Promotes nucleolar location of PML.
Subcellular location: Nucleus, nucleolus {ECO:0000269|PubMed:15195100, ECO:0000269|PubMed:21804542}. Cytoplasm {ECO:0000269|PubMed:21804542, ECO:0000269|PubMed:36517592}.
Sequence: MAQDQGEKENPMRELRIRKLCLNICVGESGDRLTRAAKVLEQLTGQTPVFSKARYTVRSFGIRRNEKIAVHCTVRGAKAEEILEKGLKVREYELRKNNFSDTGNFGFGIQEHIDLGIKYDPSIGIYGLDFYVVLGRPGFSIADKKRRTGCIGAKHRISKEEAMRWFQQKYDGIILPGK
InterPro domains: - IPR002132: Large ribosomal subunit protein uL5 (family) [1-176]
- IPR031309: Large ribosomal subunit protein uL5, C-terminal (domain) [67-165]
- IPR057266: Large ribosomal subunit protein uL5, eukaryotic/archaeal-type (family) [5-171]
- IPR020929: Large ribosomal subunit protein uL5, conserved site (conserved_site) [40-56]
- IPR022803: Large ribosomal subunit protein uL5 domain superfamily (homologous_superfamily) [1-178]
- IPR031310: Large ribosomal subunit protein uL5, N-terminal (domain) [10-63]
Interaction partners: - Large ribosomal subunit protein bL12m
- Large ribosomal subunit protein uL22
- Small ribosomal subunit protein uS15
- Small ribosomal subunit protein uS3
- Small ribosomal subunit protein uS10
- Large ribosomal subunit protein uL24
- Elongation factor 2
- Small ribosomal subunit protein uS7
- Large ribosomal subunit protein bL20m
- Small ribosomal subunit protein eS10
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0042592 (homeostatic process), GO:0009987 (cellular process), GO:0048878 (chemical homeostasis), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0008219 (cell death), GO:0006807 (nitrogen compound metabolic process), GO:0048583 (regulation of response to stimulus), GO:0002682 (regulation of immune system process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0002831 (regulation of response to biotic stimulus), GO:0012501 (programmed cell death), GO:0050776 (regulation of immune response), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0010941 (regulation of cell death), GO:0019538 (protein metabolic process), GO:0033500 (carbohydrate homeostasis), GO:0032101 (regulation of response to external stimulus), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0031347 (regulation of defense response), GO:0006412 (translation), GO:0009059 (macromolecule biosynthetic process), GO:0045088 (regulation of innate immune response), GO:0006518 (peptide metabolic process), GO:0043067 (regulation of programmed cell death), GO:0042593 (glucose homeostasis), GO:0006915 (apoptotic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0010467 (gene expression), GO:0042981 (regulation of apoptotic process), GO:0043043 (peptide biosynthetic process), GO:0006414 (translational elongation), GO:0042593 (glucose homeostasis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:1990904 (ribonucleoprotein complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0030054 (cell junction), GO:0044391 (ribosomal subunit), GO:0043229 (intracellular organelle), GO:0045202 (synapse), GO:0022626 (cytosolic ribosome), GO:0043228 (non-membrane-bounded organelle), GO:0015934 (large ribosomal subunit), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0022625 (cytosolic large ribosomal subunit), GO:0005840 (ribosome)