Protein: ELAV-like protein 1 (Hu-antigen R) (HuR) (Q15717)
Organism: Homo sapiens (Human)
Function: RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity). Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:14517288, ECO:0000269|PubMed:17632515, ECO:0000269|PubMed:18285462, ECO:0000269|PubMed:19029303}. Nucleus {ECO:0000269|PubMed:14517288, ECO:0000269|PubMed:17632515, ECO:0000269|PubMed:18285462}. Cytoplasm, Stress granule {ECO:0000250|UniProtKB:P70372}. Cytoplasm, P-body {ECO:0000269|PubMed:29476152}. Note=Translocates into the cytoplasm following phosphorylation by MAPKAPK2 (PubMed:14517288). Likewise, phosphorylation by PRKCD promotes translocation from the nucleus into the cytoplasm, where it is associated with free and cytoskeleton-bound polysomes (PubMed:18285462). Localizes to the stress granules in the presence of PLEKHN1 (By similarity). {ECO:0000250|UniProtKB:P70372, ECO:0000269|PubMed:14517288, ECO:0000269|PubMed:18285462}.
Sequence: MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK
InterPro domains: - IPR035979: RNA-binding domain superfamily (homologous_superfamily) [18-324]
- IPR012677: Nucleotide-binding alpha-beta plait domain superfamily (homologous_superfamily) [1-326]
- IPR034996: HuR, RNA recognition motif 2 (domain) [106-189]
- IPR002343: Paraneoplastic encephalomyelitis antigen (family) [20-184]
- IPR000504: RNA recognition motif domain (domain) [20-322]
- IPR006548: Splicing factor ELAV/Hu (family) [19-223]
Interaction partners: - Protein argonaute-2
- Polyadenylate-binding protein 2
- Ubiquitin-fold modifier-conjugating enzyme 1
- Insulin-like growth factor 2 mRNA-binding protein 1
- Heterogeneous nuclear ribonucleoproteins C1/C2
- Cytotoxic granule associated RNA binding protein TIA1
- Polypyrimidine tract-binding protein 1
- Nucleolysin TIAR
- Acidic leucine-rich nuclear phosphoprotein 32 family member A
- Heterogeneous nuclear ribonucleoprotein D0
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:1901363 (heterocyclic compound binding), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0003727 (single-stranded RNA binding), GO:0003729 (mRNA binding), GO:0036002 (pre-mRNA binding), GO:0097157 (pre-mRNA intronic binding), GO:0070717 (poly-purine tract binding), GO:0003730 (mRNA 3'-UTR binding), GO:0035925 (mRNA 3'-UTR AU-rich region binding), GO:0008143 (poly(A) binding)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0051641 (cellular localization), GO:0019222 (regulation of metabolic process), GO:0051234 (establishment of localization), GO:0051239 (regulation of multicellular organismal process), GO:0033036 (macromolecule localization), GO:0009892 (negative regulation of metabolic process), GO:0065008 (regulation of biological quality), GO:0050793 (regulation of developmental process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0048523 (negative regulation of cellular process), GO:0048522 (positive regulation of cellular process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0043487 (regulation of RNA stability), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0051649 (establishment of localization in cell), GO:0060255 (regulation of macromolecule metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:0045184 (establishment of protein localization), GO:0009894 (regulation of catabolic process), GO:0046907 (intracellular transport), GO:0031325 (positive regulation of cellular metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0006810 (transport), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0070727 (cellular macromolecule localization), GO:0031323 (regulation of cellular metabolic process), GO:0009895 (negative regulation of catabolic process), GO:0080090 (regulation of primary metabolic process), GO:2000036 (regulation of stem cell population maintenance), GO:0071705 (nitrogen compound transport), GO:0034250 (positive regulation of amide metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0043488 (regulation of mRNA stability), GO:0072594 (establishment of protein localization to organelle), GO:0031330 (negative regulation of cellular catabolic process), GO:0071702 (organic substance transport), GO:0051169 (nuclear transport), GO:0010628 (positive regulation of gene expression), GO:0010468 (regulation of gene expression), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0006886 (intracellular protein transport), GO:0034248 (regulation of amide metabolic process), GO:0043489 (RNA stabilization), GO:0015031 (protein transport), GO:0031326 (regulation of cellular biosynthetic process), GO:0051247 (positive regulation of protein metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0008104 (protein localization), GO:0051252 (regulation of RNA metabolic process), GO:0031329 (regulation of cellular catabolic process), GO:0051253 (negative regulation of RNA metabolic process), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0010629 (negative regulation of gene expression), GO:0006606 (protein import into nucleus), GO:1902373 (negative regulation of mRNA catabolic process), GO:0006913 (nucleocytoplasmic transport), GO:0033365 (protein localization to organelle), GO:0061013 (regulation of mRNA catabolic process), GO:1903312 (negative regulation of mRNA metabolic process), GO:0016441 (post-transcriptional gene silencing), GO:1902369 (negative regulation of RNA catabolic process), GO:0048255 (mRNA stabilization), GO:2000112 (regulation of cellular macromolecule biosynthetic process), GO:0006417 (regulation of translation), GO:1903311 (regulation of mRNA metabolic process), GO:0045727 (positive regulation of translation), GO:0010608 (post-transcriptional regulation of gene expression), GO:0034504 (protein localization to nucleus), GO:0051170 (import into nucleus), GO:0070935 (3'-UTR-mediated mRNA stabilization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:1990904 (ribonucleoprotein complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0030054 (cell junction), GO:0098794 (postsynapse), GO:0005615 (extracellular space), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0005576 (extracellular region), GO:0099080 (supramolecular complex), GO:0036464 (cytoplasmic ribonucleoprotein granule), GO:0043229 (intracellular organelle), GO:0045202 (synapse), GO:0043230 (extracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0035770 (ribonucleoprotein granule), GO:0099572 (postsynaptic specialization), GO:0035770 (ribonucleoprotein granule), GO:0043233 (organelle lumen), GO:0005783 (endoplasmic reticulum), GO:0070161 (anchoring junction), GO:0070062 (extracellular exosome), GO:0043227 (membrane-bounded organelle), GO:0030055 (cell-substrate junction), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0014069 (postsynaptic density), GO:0065010 (extracellular membrane-bounded organelle), GO:0098984 (neuron to neuron synapse), GO:0031982 (vesicle), GO:0070013 (intracellular organelle lumen), GO:0005925 (focal adhesion), GO:0032279 (asymmetric synapse), GO:1903561 (extracellular vesicle), GO:0005634 (nucleus), GO:0031981 (nuclear lumen)