Protein: Serine palmitoyltransferase 1 (Q502G6)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. The SPT complex is also composed of SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer with SPTLC2 or SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1- SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3- SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Required for adipocyte cell viability and metabolic homeostasis
Sequence: MASGQQWVLVEMVQAFYEAPAYHLILEGFLILWIIRLLFSKTYKLQERSDLTEKEKEELIEEWQPEPLVSPVSKDHPSLNYDVVTGPPSHKIIVNGKECINFASFNFLGLLDNERVKLKALASLKKYGVGTCGPRGFYGTFDVHLELEERLAKFMRTEEAIIYSYGFATIASAIPAYSKRGDIIFVDEAACFSIQKGLQASRSFIKYFKHNDMEDLERLLKEQEIEDQKNPRKARVIRRFILVEGLYINTADICPLPELVKLKYKYKVRIFLEESMSFGVLGEHGRGVTEHFGVNIDDIDLISANMENALASIGGFCCGRSFVIDHQRLSGQGYCFSASLPPMLAAAAIEALNIMEEDPGIFRVLREKCRNVHKALQGTAGLKVVGESFAPALHLQLENSTGSRVNDLKLLRAIVDYSLDRQVALTLARYLDKEERFLPPPSIRVVVTVEQTQEEIEKAAQCIREAALHVLK
InterPro domains: - IPR050087: 8-amino-7-oxononanoate synthase class-II (family) [22-465]
- IPR015424: Pyridoxal phosphate-dependent transferase (homologous_superfamily) [51-466]
- IPR004839: Aminotransferase, class I/classII, large domain (domain) [98-461]
- IPR015422: Pyridoxal phosphate-dependent transferase, small domain (homologous_superfamily) [92-462]
- IPR015421: Pyridoxal phosphate-dependent transferase, major domain (homologous_superfamily) [113-358]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016746 (acyltransferase activity), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups), GO:0016408 (C-acyltransferase activity), GO:0016409 (palmitoyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044238 (primary metabolic process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0019637 (organophosphate metabolic process), GO:0006793 (phosphorus metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0043604 (amide biosynthetic process), GO:0006665 (sphingolipid metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0046467 (membrane lipid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0006644 (phospholipid metabolic process), GO:0006643 (membrane lipid metabolic process), GO:0006684 (sphingomyelin metabolic process), GO:0090407 (organophosphate biosynthetic process), GO:0030148 (sphingolipid biosynthetic process), GO:0008654 (phospholipid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140534 (endoplasmic reticulum protein-containing complex), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005783 (endoplasmic reticulum), GO:1990234 (transferase complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)