Protein: H/ACA ribonucleoprotein complex subunit GAR1 (Glycine/arginine-rich protein 1) (snoRNP protein GAR1) (P28007)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Non-catalytic component of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP), which catalyzes pseudouridylation of rRNA and is required for ribosome biogenesis. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs. The H/ACA snoRNP complex also mediates pseudouridylation of other types of RNAs. The H/ACA snoRNP complex mediates pseudouridylation at position 93 in U2 snRNA. Essential for growth.
Subcellular location: Nucleus, nucleolus {ECO:0000269|PubMed:12756332, ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:9843512}.
Sequence: MSFRGGNRGGRGGFRGGFRGGRTGSARSFQQGPPDTVLEMGAFLHPCEGDIVCRSINTKIPYFNAPIYLENKTQVGKVDEILGPLNEVFFTIKCGDGVQATSFKEGDKFYIAADKLLPIERFLPKPKVVGPPKPKNKKKRSGAPGGRGGASMGRGGSRGGFRGGRGGSSFRGGRGGSSFRGGSRGGSFRGGSRGGSRGGFRGGRR
InterPro domains: - IPR038664: H/ACA RNP complex subunit Gar1/Naf1, Cbf5-binding domain (homologous_superfamily) [33-124]
- IPR009000: Translation protein, beta-barrel domain superfamily (homologous_superfamily) [39-111]
- IPR007504: H/ACA ribonucleoprotein complex, subunit Gar1/Naf1 (family) [37-123]
Interaction partners: - H/ACA ribonucleoprotein complex subunit CBF5
- H/ACA ribonucleoprotein complex subunit NHP2
- rRNA 2'-O-methyltransferase fibrillarin
- H/ACA ribonucleoprotein complex subunit NOP10
- Nucleolar protein 58
- Nucleolar protein 56
- Protein SHQ1
- 13 kDa ribonucleoprotein-associated protein
- H/ACA ribonucleoprotein complex non-core subunit NAF1
- tRNA pseudouridine synthase 4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0044238 (primary metabolic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0042254 (ribosome biogenesis), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0034470 (ncRNA processing), GO:0006364 (rRNA processing), GO:0034660 (ncRNA metabolic process), GO:0016072 (rRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:1990904 (ribonucleoprotein complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0030684 (preribosome), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0030687 (preribosome, large subunit precursor), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus)