Protein: Chaperone protein ClpB3, chloroplastic (ATP-dependent Clp protease ATP-binding subunit ClpB homolog 3) (Casein lytic proteinase B3) (Protein ALBINO OR PALE GREEN 6) (Q9LF37)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Molecular chaperone essential for chloroplast development and seedling viability. Mediates internal thylakoid membrane formation and confers thermotolerance to chloroplasts during heat stress.
Subcellular location: Plastid, chloroplast {ECO:0000269|PubMed:16995899, ECO:0000269|PubMed:17144892}.
Sequence: MATATTTATAAFSGVVSVGTETRRIYSFSHLQPSAAFPAKPSSFKSLKLKQSARLTRRLDHRPFVVRCEASSSNGRLTQQEFTEMAWQSIVSSPDVAKENKQQIVETEHLMKALLEQKNGLARRIFSKIGVDNTKVLEATEKFIQRQPKVYGDAAGSMLGRDLEALFQRARQFKKDLKDSYVSVEHLVLAFADDKRFGKQLFKDFQISERSLKSAIESIRGKQSVIDQDPEGKYEALEKYGKDLTAMAREGKLDPVIGRDDEIRRCIQILSRRTKNNPVLIGEPGVGKTAISEGLAQRIVQGDVPQALMNRKLISLDMGALIAGAKYRGEFEDRLKAVLKEVTDSEGQIILFIDEIHTVVGAGATNGAMDAGNLLKPMLGRGELRCIGATTLDEYRKYIEKDPALERRFQQVYVDQPTVEDTISILRGLRERYELHHGVRISDSALVEAAILSDRYISGRFLPDKAIDLVDEAAAKLKMEITSKPTALDELDRSVIKLEMERLSLTNDTDKASRERLNRIETELVLLKEKQAELTEQWEHERSVMSRLQSIKEEIDRVNLEIQQAEREYDLNRAAELKYGSLNSLQRQLNEAEKELNEYLSSGKSMFREEVLGSDIAEIVSKWTGIPVSKLQQSERDKLLHLEEELHKRVVGQNPAVTAVAEAIQRSRAGLSDPGRPIASFMFMGPTGVGKTELAKALASYMFNTEEALVRIDMSEYMEKHAVSRLIGAPPGYVGYEEGGQLTETVRRRPYSVILFDEIEKAHGDVFNVFLQILDDGRVTDSQGRTVSFTNTVIIMTSNVGSQFILNNTDDDANELSYETIKERVMNAARSIFRPEFMNRVDEYIVFKPLDREQINRIVRLQLARVQKRIADRKMKINITDAAVDLLGSLGYDPNYGARPVKRVIQQNIENELAKGILRGDFKEEDGILIDTEVTAFSNGQLPQQKLTFKKIESETADAEQEEAAFSK
InterPro domains: - IPR018368: ClpA/B, conserved site 1 (conserved_site) [370-382]
- IPR028299: ClpA/B, conserved site 2 (conserved_site) [711-729]
- IPR036628: Clp, N-terminal domain superfamily (homologous_superfamily) [78-232]
- IPR003593: AAA+ ATPase domain (domain) [274-843]
- IPR019489: Clp ATPase, C-terminal (domain) [850-944]
- IPR003959: ATPase, AAA-type, core (domain) [279-843]
- IPR050130: ATP-dependent Clp protease/Chaperone ClpA/ClpB (family) [86-956]
- IPR004176: Clp, repeat (R) domain (domain) [78-222]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [225-930]
- IPR041546: ClpA/ClpB, AAA lid domain (domain) [418-520]
- IPR017730: Chaperonin ClpB (family) [82-934]
- IPR001270: ClpA/B family (family) [681-802]
Interaction partners: - ATP-dependent Clp protease proteolytic subunit 3, chloroplastic
- ATP-dependent Clp protease proteolytic subunit-related protein 1, chloroplastic
- ATP-dependent Clp protease proteolytic subunit 4, chloroplastic
- Heat shock 70 kDa protein 4
- ATP-dependent Clp protease proteolytic subunit-related protein 2, chloroplastic
- Heat shock protein 90-5, chloroplastic
- Heat shock 70 kDa protein 6, chloroplastic
- ATP-dependent Clp protease proteolytic subunit 6, chloroplastic
- Chaperonin CPN60, mitochondrial
- Heat shock 70 kDa protein 5
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0051716 (cellular response to stimulus), GO:0009628 (response to abiotic stimulus), GO:0006950 (response to stress), GO:0042221 (response to chemical), GO:0009408 (response to heat), GO:0009266 (response to temperature stimulus), GO:0001666 (response to hypoxia), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0070482 (response to oxygen levels), GO:0071456 (cellular response to hypoxia), GO:0071453 (cellular response to oxygen levels), GO:0036293 (response to decreased oxygen levels), GO:0036294 (cellular response to decreased oxygen levels)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0009532 (plastid stroma), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0005737 (cytoplasm), GO:0031967 (organelle envelope), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009570 (chloroplast stroma), GO:0009526 (plastid envelope), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle), GO:0009941 (chloroplast envelope)