Protein: Serine protease 7 (EC 3.4.21.-) (Melanization protease 2) (Q9V3Z2)
Organism: Drosophila melanogaster (Fruit fly)
Function: Serine protease which, by cleaving and activating prophenoloxidase (PPO1) after immune challenge, plays an essential role in the melanization immune response to septic wounding. May function in diverse Hayan-dependent PPO1-activating cascades that are negatively controlled by different serpin proteins; Spn27A in the hemolymph and Spn77BA in the trachea. Important for the innate immune response to fungi. Regulation of melanization and PPO1 activation appears to be largely independent of the Toll signaling pathway.
Subcellular location: Secreted {ECO:0000269|PubMed:24260243}.
Sequence: MKSTRKVVGIFLATCLLPFTVLQNVAAQGSCRNPNQKQGQCLSIYDCQSLLSVIQQSYVSPEDRTFLRNSQCLDGVGRQPYVCCTSDRSFGSQEATSAAPPPTTTSSSSRGQDGQAGLGNLLPSPPKCGPHSFSNKVYNGNDTAIDEFNWMALLEYVDNRGRRELSCGGSLINNRYVLTAAHCVIGAVETEVGHLTTVRLGEYDTSKDVDCIDDICNQPILQLGIEQATVHPQYDPANKNRIHDIALLRLDRPVVLNEYIQPVCLPLVSTRMAINTGELLVVSGWGRTTTARKSTIKQRLDLPVNDHDYCARKFATRNIHLISSQLCVGGEFYRDSCDGDSGGPLMRRGFDQAWYQEGVVSFGNRCGLEGWPGVYTRVADYMDWIVETIRP
InterPro domains: - IPR001314: Peptidase S1A, chymotrypsin family (family) [168-346]
- IPR043504: Peptidase S1, PA clan, chymotrypsin-like fold (homologous_superfamily) [150-385]
- IPR038565: Proteinase, regulatory CLIP domain superfamily (homologous_superfamily) [28-94]
- IPR001254: Serine proteases, trypsin domain (domain) [136-390]
- IPR018114: Serine proteases, trypsin family, histidine active site (active_site) [178-183]
- IPR033116: Serine proteases, trypsin family, serine active site (active_site) [335-346]
- IPR022700: Proteinase, regulatory CLIP domain (domain) [30-85]
- IPR009003: Peptidase S1, PA clan (homologous_superfamily) [120-390]
- IPR051487: Serine/Threonine Proteases with Immune and Developmental Roles (family) [42-390]
Interaction partners: - Serine protease inhibitor 27A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0017171 (serine hydrolase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0002376 (immune system process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009605 (response to external stimulus), GO:0048584 (positive regulation of response to stimulus), GO:0019222 (regulation of metabolic process), GO:0006955 (immune response), GO:0006807 (nitrogen compound metabolic process), GO:0002684 (positive regulation of immune system process), GO:0002682 (regulation of immune system process), GO:0071704 (organic substance metabolic process), GO:0009607 (response to biotic stimulus), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0009893 (positive regulation of metabolic process), GO:0006950 (response to stress), GO:0051707 (response to other organism), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0032103 (positive regulation of response to external stimulus), GO:1901564 (organonitrogen compound metabolic process), GO:0043207 (response to external biotic stimulus), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0080134 (regulation of response to stress), GO:0019538 (protein metabolic process), GO:0006952 (defense response), GO:0060255 (regulation of macromolecule metabolic process), GO:0032101 (regulation of response to external stimulus), GO:0002700 (regulation of production of molecular mediator of immune response), GO:0043170 (macromolecule metabolic process), GO:0045087 (innate immune response), GO:0009891 (positive regulation of biosynthetic process), GO:0098542 (defense response to other organism), GO:0002831 (regulation of response to biotic stimulus), GO:0050776 (regulation of immune response), GO:0031325 (positive regulation of cellular metabolic process), GO:0002702 (positive regulation of production of molecular mediator of immune response), GO:0002697 (regulation of immune effector process), GO:0002833 (positive regulation of response to biotic stimulus), GO:0009617 (response to bacterium), GO:0009889 (regulation of biosynthetic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0050778 (positive regulation of immune response), GO:0031323 (regulation of cellular metabolic process), GO:0031349 (positive regulation of defense response), GO:0002699 (positive regulation of immune effector process), GO:0009620 (response to fungus), GO:0031347 (regulation of defense response), GO:0002784 (regulation of antimicrobial peptide production), GO:0006508 (proteolysis), GO:0034250 (positive regulation of amide metabolic process), GO:1900426 (positive regulation of defense response to bacterium), GO:0002922 (positive regulation of humoral immune response), GO:0002760 (positive regulation of antimicrobial humoral response), GO:0051604 (protein maturation), GO:0010628 (positive regulation of gene expression), GO:0002225 (positive regulation of antimicrobial peptide production), GO:1900424 (regulation of defense response to bacterium), GO:0010468 (regulation of gene expression), GO:0034248 (regulation of amide metabolic process), GO:0002759 (regulation of antimicrobial humoral response), GO:0050832 (defense response to fungus), GO:0042742 (defense response to bacterium), GO:0031326 (regulation of cellular biosynthetic process), GO:1900150 (regulation of defense response to fungus), GO:0002920 (regulation of humoral immune response), GO:0010467 (gene expression), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0002803 (positive regulation of antibacterial peptide production), GO:0002805 (regulation of antimicrobial peptide biosynthetic process), GO:0002786 (regulation of antibacterial peptide production), GO:0072376 (protein activation cascade), GO:0050829 (defense response to Gram-negative bacterium), GO:0050830 (defense response to Gram-positive bacterium), GO:0002807 (positive regulation of antimicrobial peptide biosynthetic process), GO:0160032 (Toll receptor ligand protein activation cascade), GO:0002808 (regulation of antibacterial peptide biosynthetic process), GO:0006963 (positive regulation of antibacterial peptide biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005576 (extracellular region), GO:0005615 (extracellular space)