Protein: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (B6JX81)
Organism: Schizosaccharomyces japonicus (strain yFS275 / FY16936) (Fission yeast)
Function: Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol- pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity
Subcellular location: Endoplasmic reticulum membrane; Single- pass type I membrane protein
Sequence: MWSLRVLVLTAACGLFSSVNAWKNTNLIRTYDLTKSYVKETITIKLENDLQEAANVYTLMKDLVDDERVVSAVALEKTQGGSVSLDIVPSLTTEDGLDIRFPSPVLPGESKTIHVSLTLDGAQKPVPAKIKQDDQQYLVYRASKTFVSPYDTEKQRLKFKFPTNNVPSFTEFTDASGNKQPARAGNTLTYETLETVERDTPKELVSVRYEYTAPLPRARQLDIEVDIHQFRKEISVVEKTTLENHAAELRGHFNRAKWAMSRFYNPLTAALGSFRIYLPKDVRDVSFKDEDGNVTTSHMRADRQHTVLDLIPRFPVFGGWNYYFRLAWNMALDSFAADKNGRTVVTLPSMWSLEDALFSNVSWTLTLPEGAKDIKVNVPTELSLKNVTTVYHFLDIQGRQSFVYEASDVSDEFSQKVIEISYVYDTLAHRLRLAVIVGIIGVLFTAAYMIWAP
InterPro domains: - IPR007676: Ribophorin I (family) [3-450]
Interaction partners: - Oligosaccharyltransferase gamma subunit
- Oligosaccharyltransferase delta subunit
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
- Translocon gamma subunit Sss1
- Translocon alpha subunit Sec61
- Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
- Translocon subunit Sec61
- Protein transport protein Sec61 subunit beta
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0070085 (glycosylation), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0043413 (macromolecule glycosylation), GO:1901135 (carbohydrate derivative metabolic process), GO:0036211 (protein modification process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0006486 (protein glycosylation), GO:0043412 (macromolecule modification), GO:0009100 (glycoprotein metabolic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0006487 (protein N-linked glycosylation), GO:0009101 (glycoprotein biosynthetic process), GO:0018279 (protein N-linked glycosylation via asparagine)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)