Protein: Homeobox protein NANOG (Homeobox transcription factor Nanog) (hNanog) (Q9H9S0)
Organism: Homo sapiens (Human)
Function: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Subcellular location: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000269|PubMed:15983365}.
Sequence: MSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNNWPKNSNGVTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQTWNNSTWSNQTQNIQSWSNHSWNTQTWCTQSWNNQAWNSPFYNCGEESLQSCMQFQPNSPASDLEAALEAAGEGLNVIQQTTRYFSTPQTMDLFLNYSMNMQPEDV
InterPro domains: - IPR001356: Homeodomain (domain) [93-157]
- IPR009057: Homedomain-like superfamily (homologous_superfamily) [90-155]
- IPR017970: Homeobox, conserved site (conserved_site) [128-151]
- IPR050460: Distal-less homeobox transcription factors (family) [7-234]
Interaction partners: - Sal-like protein 4
- POU domain, class 5, transcription factor 1
- Transcription factor SOX-2
- Signal transducer and activator of transcription 3
- Krueppel-like factor 4
- Methylcytosine dioxygenase TET1
- CD44 antigen
- Protein lin-28 homolog A
- Homeobox protein NANOGP8
- PR domain zinc finger protein 14
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:0003700 (DNA-binding transcription factor activity), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0050794 (regulation of cellular process), GO:0048522 (positive regulation of cellular process), GO:0051726 (regulation of cell cycle), GO:0090068 (positive regulation of cell cycle process), GO:0042127 (regulation of cell population proliferation), GO:0010564 (regulation of cell cycle process), GO:0008284 (positive regulation of cell population proliferation), GO:0045787 (positive regulation of cell cycle), GO:1901989 (positive regulation of cell cycle phase transition), GO:1901987 (regulation of cell cycle phase transition), GO:1902806 (regulation of cell cycle G1/S phase transition), GO:1902808 (positive regulation of cell cycle G1/S phase transition)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)