Protein: Mitochondrial tRNA-specific 2-thiouridylase 1 (EC 2.8.1.14) (Q9DAT5)
Organism: Mus musculus (Mouse)
Function: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.
Subcellular location: Mitochondrion {ECO:0000269|PubMed:14746906}.
Sequence: MSALRHVVCALSGGVDSAVAALLLRRRGYQVTGVFMKNWDSLDEQGVCAADKDCEDAYKVCQILDIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDINCNKHIKFSCFYHYAVDNLGADAVATGHYARTSLEDEEVFEQKHTKKPDGLFRNRFEVRNPVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGELTKDFVKKIAAENSLHHVLQKRESMGICFIGKRNLEHFLLQYLQPRPGKFVSIEDNTVLGTHKGWFLYTLGQRAKISGLREPWYVVEKDGTKGDVLVAPRVDHPALYRDLLRTNRVHWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVKAVRGLALGQFAVFYKGEECLGSGKILRLGPSAYTLQKGKNRTRVAPEASSDSPGLHPTS
InterPro domains: - IPR004506: tRNA-specific 2-thiouridylase MnmA-like (family) [5-384]
- IPR023382: tRNA-specific 2-thiouridylase MnmA-like, central domain superfamily (homologous_superfamily) [236-299]
- IPR046885: tRNA-specific 2-thiouridylase MnmA-like, C-terminal domain (domain) [303-383]
- IPR046884: tRNA-specific 2-thiouridylase MnmA-like, central domain (domain) [230-292]
- IPR014729: Rossmann-like alpha/beta/alpha sandwich fold (homologous_superfamily) [2-234]
Interaction partners: - 5-taurinomethyluridine-[tRNA] synthase subunit MTO1, mitochondrial
- 5-taurinomethyluridine-[tRNA] synthase subunit GTPB3, mitochondrial
- tRNA dimethylallyltransferase
- Elongation factor Ts, mitochondrial
- Cytoplasmic tRNA 2-thiolation protein 1
- Pseudouridylate synthase 1 homolog
- Methionyl-tRNA formyltransferase, mitochondrial
- Cysteine desulfurase
- Adenylyltransferase and sulfurtransferase MOCS3
- Elongation factor Tu, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016782 (transferase activity, transferring sulphur-containing groups), GO:0016783 (sulfurtransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0140053 (mitochondrial gene expression), GO:0006396 (RNA processing), GO:0009451 (RNA modification), GO:1900864 (mitochondrial RNA modification), GO:0000963 (mitochondrial RNA processing), GO:0034470 (ncRNA processing), GO:0000959 (mitochondrial RNA metabolic process), GO:0006400 (tRNA modification), GO:0070525 (tRNA threonylcarbamoyladenosine metabolic process), GO:0034660 (ncRNA metabolic process), GO:0090646 (mitochondrial tRNA processing), GO:0034227 (tRNA thio-modification), GO:0006399 (tRNA metabolic process), GO:0008033 (tRNA processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)