Protein: Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase (P0ADR8)
Organism: Escherichia coli (strain K12)
Function: Catalyzes the hydrolysis of the N-glycosidic bond of diverse pyrimidine and purine nucleotide 5'-monophosphates, to form ribose 5- phosphate and the corresponding free base. Can use AMP, GMP, IMP, CMP, dTMP and UMP as substrates. Cannot catalyze the reverse reactions. Is required for optimal growth in glucose minimal medium, possibly because it contributes to nucleoside pool homeostasis by degrading excess nucleotides and feeding back the ribose moiety to catabolism
Sequence: MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNSGSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLRDILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSINENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPSIIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPANKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMPLVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAFSGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCYEICT
InterPro domains: - IPR049788: Nucleotide 5'-monophosphate nucleosidase PpnN (family) [3-452]
- IPR021826: Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase, C-terminal domain (domain) [333-452]
- IPR027820: Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase, N-terminal (domain) [4-109]
- IPR031100: LOG family (family) [190-329]
- IPR052341: LOG family pyrimidine/purine nucleotide 5'-monophosphatases (family) [47-348]
- IPR037153: Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase PpnN-like superfamily (homologous_superfamily) [1-112]
Interaction partners: - Protein UshA
- GMP/IMP nucleotidase YrfG
- AMP nucleosidase
- AMP nucleosidase
- Pyrimidine/purine nucleoside phosphorylase
- 5'/3'-nucleotidase SurE
- Adenylosuccinate lyase
- Adenylosuccinate lyase
- Adenine phosphoribosyltransferase
- Adenylate kinase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0016798 (hydrolase activity, acting on glycosyl bonds), GO:0032553 (ribonucleotide binding), GO:0016799 (hydrolase activity, hydrolyzing N-glycosyl compounds), GO:0097216 (guanosine tetraphosphate binding), GO:0032555 (purine ribonucleotide binding), GO:0017076 (purine nucleotide binding), GO:0019001 (guanyl nucleotide binding), GO:0032561 (guanyl ribonucleotide binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0044248 (cellular catabolic process), GO:0046700 (heterocycle catabolic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0009112 (nucleobase metabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0072527 (pyrimidine-containing compound metabolic process), GO:0019439 (aromatic compound catabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0046113 (nucleobase catabolic process), GO:0006206 (pyrimidine nucleobase metabolic process), GO:0072529 (pyrimidine-containing compound catabolic process), GO:0006208 (pyrimidine nucleobase catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)