Protein: Nck-associated protein 1 (NAP 1) (Membrane-associated protein HEM-2) (p125Nap1) (Q9Y2A7)
Organism: Homo sapiens (Human)
Function: Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.
Subcellular location: Cell membrane {ECO:0000250|UniProtKB:P28660}; Single-pass membrane protein {ECO:0000250|UniProtKB:P28660}; Cytoplasmic side {ECO:0000250|UniProtKB:P28660}. Cell projection, lamellipodium membrane {ECO:0000250|UniProtKB:P28660}; Single-pass membrane protein {ECO:0000250|UniProtKB:P28660}; Cytoplasmic side {ECO:0000250|UniProtKB:P28660}. Note=At the interface between the lamellipodial actin meshwork and the membrane. {ECO:0000250|UniProtKB:P28660}.
Sequence: MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA
InterPro domains: - IPR019137: Nck-associated protein 1 (family) [7-1126]
Interaction partners: - Actin-binding protein WASF3
- Abl interactor 2
- Cytoplasmic FMR1-interacting protein 2
- Actin-binding protein WASF1
- Cytoplasmic FMR1-interacting protein 1
- Abl interactor 1
- Actin-binding protein WASF2
- Protein BRICK1
- Ras-related C3 botulinum toxin substrate 1
- ABI gene family member 3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0005488 (binding), GO:0030234 (enzyme regulator activity), GO:0140677 (molecular function activator activity), GO:0044877 (protein-containing complex binding), GO:0005515 (protein binding), GO:0060589 (nucleoside-triphosphatase regulator activity), GO:0019207 (kinase regulator activity), GO:0008047 (enzyme activator activity), GO:0030695 (GTPase regulator activity), GO:0019209 (kinase activator activity), GO:0005096 (GTPase activator activity), GO:0019887 (protein kinase regulator activity), GO:0030295 (protein kinase activator activity)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0040011 (locomotion), GO:0042592 (homeostatic process), GO:0002376 (immune system process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0048856 (anatomical structure development), GO:0048870 (cell motility), GO:0042221 (response to chemical), GO:0007163 (establishment or maintenance of cell polarity), GO:0042330 (taxis), GO:0051240 (positive regulation of multicellular organismal process), GO:0002684 (positive regulation of immune system process), GO:0071840 (cellular component organization or biogenesis), GO:0051241 (negative regulation of multicellular organismal process), GO:0051239 (regulation of multicellular organismal process), GO:0048871 (multicellular organismal-level homeostasis), GO:0009892 (negative regulation of metabolic process), GO:0065008 (regulation of biological quality), GO:0002683 (negative regulation of immune system process), GO:0040017 (positive regulation of locomotion), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0002253 (activation of immune response), GO:0048523 (negative regulation of cellular process), GO:0007165 (signal transduction), GO:0040012 (regulation of locomotion), GO:0048869 (cellular developmental process), GO:0023056 (positive regulation of signaling), GO:0051051 (negative regulation of transport), GO:0048584 (positive regulation of response to stimulus), GO:0019222 (regulation of metabolic process), GO:0032879 (regulation of localization), GO:0002682 (regulation of immune system process), GO:0050900 (leukocyte migration), GO:0009893 (positive regulation of metabolic process), GO:0065009 (regulation of molecular function), GO:0030029 (actin filament-based process), GO:0002262 (myeloid cell homeostasis), GO:0048585 (negative regulation of response to stimulus), GO:0007154 (cell communication), GO:0048522 (positive regulation of cellular process), GO:0030011 (maintenance of cell polarity), GO:0050777 (negative regulation of immune response), GO:0002695 (negative regulation of leukocyte activation), GO:2000145 (regulation of cell motility), GO:0060548 (negative regulation of cell death), GO:0002685 (regulation of leukocyte migration), GO:0009410 (response to xenobiotic stimulus), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0009966 (regulation of signal transduction), GO:0030155 (regulation of cell adhesion), GO:0060255 (regulation of macromolecule metabolic process), GO:0002694 (regulation of leukocyte activation), GO:0060627 (regulation of vesicle-mediated transport), GO:0032970 (regulation of actin filament-based process), GO:0044089 (positive regulation of cellular component biogenesis), GO:0051048 (negative regulation of secretion), GO:0044087 (regulation of cellular component biogenesis), GO:0031341 (regulation of cell killing), GO:0050866 (negative regulation of cell activation), GO:0007162 (negative regulation of cell adhesion), GO:0030595 (leukocyte chemotaxis), GO:0002697 (regulation of immune effector process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0050778 (positive regulation of immune response), GO:0016043 (cellular component organization), GO:0007166 (cell surface receptor signaling pathway), GO:0002698 (negative regulation of immune effector process), GO:0051128 (regulation of cellular component organization), GO:0006935 (chemotaxis), GO:0050865 (regulation of cell activation), GO:0090066 (regulation of anatomical structure size), GO:1903531 (negative regulation of secretion by cell), GO:0008284 (positive regulation of cell population proliferation), GO:0016477 (cell migration), GO:0031342 (negative regulation of cell killing), GO:0097529 (myeloid leukocyte migration), GO:0002764 (immune response-regulating signaling pathway), GO:0009967 (positive regulation of signal transduction), GO:0050790 (regulation of catalytic activity), GO:1903530 (regulation of secretion by cell), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0051049 (regulation of transport), GO:0044092 (negative regulation of molecular function), GO:0051130 (positive regulation of cellular component organization), GO:0045785 (positive regulation of cell adhesion), GO:0030036 (actin cytoskeleton organization), GO:0031324 (negative regulation of cellular metabolic process), GO:2000147 (positive regulation of cell motility), GO:0050867 (positive regulation of cell activation), GO:0002687 (positive regulation of leukocyte migration), GO:0048872 (homeostasis of number of cells), GO:0002696 (positive regulation of leukocyte activation), GO:0050776 (regulation of immune response), GO:0042127 (regulation of cell population proliferation), GO:0002757 (immune response-activating signaling pathway), GO:0010941 (regulation of cell death), GO:0034101 (erythrocyte homeostasis), GO:0010646 (regulation of cell communication), GO:0031325 (positive regulation of cellular metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0010647 (positive regulation of cell communication), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0043300 (regulation of leukocyte degranulation), GO:0071621 (granulocyte chemotaxis), GO:0032535 (regulation of cellular component size), GO:0097530 (granulocyte migration), GO:0043254 (regulation of protein-containing complex assembly), GO:0002703 (regulation of leukocyte mediated immunity), GO:0051246 (regulation of protein metabolic process), GO:0022409 (positive regulation of cell-cell adhesion), GO:0043069 (negative regulation of programmed cell death), GO:0043067 (regulation of programmed cell death), GO:0022407 (regulation of cell-cell adhesion), GO:0002704 (negative regulation of leukocyte mediated immunity), GO:0032956 (regulation of actin cytoskeleton organization), GO:0030334 (regulation of cell migration), GO:1902622 (regulation of neutrophil migration), GO:0070663 (regulation of leukocyte proliferation), GO:0051250 (negative regulation of lymphocyte activation), GO:1902903 (regulation of supramolecular fiber organization), GO:0002429 (immune response-activating cell surface receptor signaling pathway), GO:0051251 (positive regulation of lymphocyte activation), GO:1902905 (positive regulation of supramolecular fiber organization), GO:0010563 (negative regulation of phosphorus metabolic process), GO:0030335 (positive regulation of cell migration), GO:1902533 (positive regulation of intracellular signal transduction), GO:0070665 (positive regulation of leukocyte proliferation), GO:0022408 (negative regulation of cell-cell adhesion), GO:0002819 (regulation of adaptive immune response), GO:0051249 (regulation of lymphocyte activation), GO:0045920 (negative regulation of exocytosis), GO:0030097 (hemopoiesis), GO:0043086 (negative regulation of catalytic activity), GO:0002768 (immune response-regulating cell surface receptor signaling pathway), GO:0010562 (positive regulation of phosphorus metabolic process), GO:0001911 (negative regulation of leukocyte mediated cytotoxicity), GO:0051336 (regulation of hydrolase activity), GO:0051248 (negative regulation of protein metabolic process), GO:0017157 (regulation of exocytosis), GO:1902624 (positive regulation of neutrophil migration), GO:0030099 (myeloid cell differentiation), GO:0002820 (negative regulation of adaptive immune response), GO:0060326 (cell chemotaxis), GO:0030218 (erythrocyte differentiation), GO:0030866 (cortical actin cytoskeleton organization), GO:0001910 (regulation of leukocyte mediated cytotoxicity), GO:0033043 (regulation of organelle organization), GO:0051046 (regulation of secretion), GO:0006996 (organelle organization), GO:0010638 (positive regulation of organelle organization), GO:1902531 (regulation of intracellular signal transduction), GO:0051174 (regulation of phosphorus metabolic process), GO:0031334 (positive regulation of protein-containing complex assembly), GO:0061515 (myeloid cell development), GO:0032006 (regulation of TOR signaling), GO:0050670 (regulation of lymphocyte proliferation), GO:0042981 (regulation of apoptotic process), GO:0051493 (regulation of cytoskeleton organization), GO:0002822 (regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains), GO:0050863 (regulation of T cell activation), GO:0051346 (negative regulation of hydrolase activity), GO:0032944 (regulation of mononuclear cell proliferation), GO:0032271 (regulation of protein polymerization), GO:0031400 (negative regulation of protein modification process), GO:0010921 (regulation of phosphatase activity), GO:0045937 (positive regulation of phosphate metabolic process), GO:1990266 (neutrophil migration), GO:0050671 (positive regulation of lymphocyte proliferation), GO:0007010 (cytoskeleton organization), GO:0032946 (positive regulation of mononuclear cell proliferation), GO:1903306 (negative regulation of regulated secretory pathway), GO:1903038 (negative regulation of leukocyte cell-cell adhesion), GO:0030838 (positive regulation of actin filament polymerization), GO:0050864 (regulation of B cell activation), GO:0050871 (positive regulation of B cell activation), GO:0032008 (positive regulation of TOR signaling), GO:0002707 (negative regulation of lymphocyte mediated immunity), GO:0050870 (positive regulation of T cell activation), GO:0031399 (regulation of protein modification process), GO:0030593 (neutrophil chemotaxis), GO:0043066 (negative regulation of apoptotic process), GO:0030832 (regulation of actin filament length), GO:0001914 (regulation of T cell mediated cytotoxicity), GO:0019220 (regulation of phosphate metabolic process), GO:0050851 (antigen receptor-mediated signaling pathway)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0031209 (SCAR complex), GO:0071944 (cell periphery), GO:0005615 (extracellular space), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005576 (extracellular region), GO:0031090 (organelle membrane), GO:0030141 (secretory granule), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043230 (extracellular organelle), GO:0070062 (extracellular exosome), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0101002 (ficolin-1-rich granule), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0012506 (vesicle membrane), GO:0065010 (extracellular membrane-bounded organelle), GO:0030667 (secretory granule membrane), GO:0070820 (tertiary granule), GO:0031982 (vesicle), GO:0030659 (cytoplasmic vesicle membrane), GO:1903561 (extracellular vesicle), GO:0097708 (intracellular vesicle), GO:0101003 (ficolin-1-rich granule membrane)