Protein: N-acetylglucosamine kinase 1 (Q59RW5)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Component of the N-acetylglucosamine catabolic cascade that phosphorylates N-acetylglucosamine (GlcNAc), and allows the unique ability to utilise GlcNAc as carbon source. Converts GlcNAc to GlcNAc- 6-P. Also able to phosphorylate glucose, glucosamine (GlcN), and mannose. Galactose, fructose, N-acetylmannosamine (ManNAc), mannosamine (ManN), galactosamine (GalN), and N-acetylgalactosamine (GalNAc) are not phosphorylated by HXK1. GlcNAc metabolism is closely associated with virulence and morphogenesis, and is involved in the cell wall synthesis. Acts both as a repressor and an activator of genes involved in maintaining cellular homeostasis. Contributes to white-opaque morphological transition and plays a role as a filamentation repressor
Subcellular location: Cytoplasm. Nucleus. Mitochondrion. Note=Localized in cytoplasm and nucleus in a filamentation-inducing medium whereas in 2% GlcNAc, where catabolism is more prominent, a major fraction is seen to be present in cytoplasm. Localizes to mitochondria in non-fermentative carbon sources like ethanol
Sequence: MTETSISGLRGPKSMYFMEIVDVSSQESSVLSSIVESFTSAVSASNLGVYSDEVLCDIKSSLKENSPITMLPNYNVSPTGDEHGQYLVIDLGGSTLRIAVVDISKPHPNLSRSERITIVVEKSWIIGNDFKRIDGEFFKYIGSKINEILMGQNVIDVKSVINTGITWSFPLETTDYNRGKIKHVSKGYTVGEDIYDKDLKMVLEDTLRQEYGLTLDVQSILNDSLAVYSAGCFIDSKMKLAMVLGTGINMCCSLKRSSDIHPSKMLADATLFNCELSLFGQNLCKDFATKYDIIIDKRFAGLSHHFKTFMEPDPITKTLFQPHELMTSGRYLPELTRLVVVDLIEAGEIFQNVDHQQMYQEYGGFSGELICFVHENDDYDDIHDKLCKAYGWTTVGLSDIVCLKEVVSCIIKRAAFIVANAIIAFFKLLGSDELGGDVTIGYVGSVLNYFHKYRRLIVEYVNSAEEAKGIKVDLKLIENSSIIGAAIGAAYHK
InterPro domains: - IPR022673: Hexokinase, C-terminal (domain) [240-490]
- IPR022672: Hexokinase, N-terminal (domain) [41-230]
- IPR043129: ATPase, nucleotide binding domain (homologous_superfamily) [30-491]
- IPR001312: Hexokinase (family) [27-491]
Interaction partners: - Glucosamine-6-phosphate isomerase
- N-acetylglucosamine-6-phosphate deacetylase
- ATP-dependent 6-phosphofructokinase
- ATP-dependent 6-phosphofructokinase
- Glucose-6-phosphate isomerase
- phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)
- Phosphotransferase
- Glucosamine 6-phosphate N-acetyltransferase
- fructose-bisphosphatase
- Glucose-6-phosphate 1-dehydrogenase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0016301 (kinase activity), GO:0019200 (carbohydrate kinase activity), GO:0004396 (hexokinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0044238 (primary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0005975 (carbohydrate metabolic process), GO:0006793 (phosphorus metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0046835 (carbohydrate phosphorylation), GO:0016310 (phosphorylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0030312 (external encapsulating structure), GO:0031012 (extracellular matrix), GO:0062039 (biofilm matrix), GO:0062040 (fungal biofilm matrix)