Protein: Nidogen (Entactin) (A1Z877)
Organism: Drosophila melanogaster (Fruit fly)
Function: Cell adhesion glycoprotein which is widely distributed in basement membranes. Involved in cell-extracellular matrix (ECM) interactions probably by connecting the laminin and collagen IV networks. Required for permeability and mechanical stability of basement membranes, and ECM dependent neural plasticity. Not involved in assembly of the embryonic basement membrane.
Subcellular location: Secreted {ECO:0000269|PubMed:30260959}. Secreted, extracellular space, extracellular matrix, basement membrane {ECO:0000269|PubMed:30260959, ECO:0000269|PubMed:30567930}. Note=Secreted in the hemolymph (PubMed:30260959). Localization to the basal membranes depends on laminin (PubMed:30260959, PubMed:30567930). {ECO:0000269|PubMed:30260959, ECO:0000269|PubMed:30567930}.
Sequence: MPTFGSKLLACLLLSSVILVSGQFEHYLDSLRASELYEFEDGSLGSIHLLPKGDSETIVLQLEQPIHFYGEQYEQLYINTNGILTFNSEFPEYLNQPFPLEYASIAAFYSNVDTSFSDEGTSISLFESKEQSILDRASSLVRYAFSSQSEFEARQVIVATWRNVGYFDSKTDRLNTFQVALIANEQSTFVQFIYPDGGLNWLQGETAGLGLPDIRAQAGFVAEDGRFYTLNGSGSENARFLSESTNLGVPGVWLFEVAPIENEQNVRSPDNAESLTESPALALSCQAHAHQCHEKAECHDKAEGYCCVCGSGFYGNGKSCLANDQPIRVTGTLTGELNKQPVSEEAKLQSYVVTSEGRTYTTINPLTPELGAQLRLVLPLLTTVPWLFAKSVGGVANGYQLTGGVYTHVSRLQFDSGENLHVNQTFEGLNYWDQLSVKIEIYGEVPAVAADAVLILPDYVEEYTFERPGELKSVQVLNINITEEQRVLGLQVEQRILYRSCLRDDEADPSATKVLQKISKVALDYVERDQALRIGAMSKVGVTPESNACNDGTADCVENSVCVPYEDTYRCDCYHGFAAQLDERGVEVCLDIDECATGSHVCDENAVCDNTEGGFNCYCTEGFEGNGYRCLSNSTADNIEYPPAVEGQAEPTSEPSPNPSPYPDQGQDQEREREDDQYPQPNPYPYPEEQIPQHPDECYRCSKDADCYQGRCTCHEGFDGDGYTCTNICGHGEVWENGRCEPLLLERHDVDPLCDALGECRCPYGYELSEDSQRCTYVQEFDGERNADLIPCDVDENCHINATCNWYGQELRHICTCQPGFRGDGYNCDPISDDSCAIRPDICDVHADCVYEEHLGKSECQCQAGYTGNGFNCQLAAECQSAEHCGENAFCDDGVCRCQADFERDVSDRCVPAGRCGSVFCGSNAICKWDSAEGVQYCDCLDGYQGDALTGCTSKPLSCHVLNNCGIHATCEPTEDPANYECQCIAGFKGDGYVCIEEQNCLNNPTLCDMNAQCRSTNSGLVCVCNQGFFGNGSLCQERQHQDSDFLIVSQGVMIARVPLNGRNVRPISVAQMAIGLDKDCVEGRVYWGDISTKKIVSTKYDGTDLRPFITTDIESPEGIAIDVISRRLYWADSAKDTIEVASLDDPSLRAVIINKQLVNPRGIAVDPYREKLFWSDWDRESPKIEMSNLDGTGRELLLGKDDVTLPNSLVVLENSGEVCYADAGTKKVECIEPQNRQIRTISNELSYPFGITFTHDQFYWTDWTTKKVEIVDSLGARQTPIQPPFFGSHKMYGMTVVEQHCPQYQSPCQISNGGCTDSRLCLVNRQAPSGKSCKCTSASTGCTVLAPGY
InterPro domains: - IPR011042: Six-bladed beta-propeller, TolB-like (homologous_superfamily) [1035-1344]
- IPR009017: Green fluorescent protein (homologous_superfamily) [292-543]
- IPR001881: EGF-like calcium-binding domain (domain) [545-631]
- IPR018097: EGF-like calcium-binding, conserved site (conserved_site) [591-617]
- IPR000152: EGF-type aspartate/asparagine hydroxylation site (ptm) [608-619]
- IPR000033: LDLR class B repeat (repeat) [1066-1279]
- IPR049883: NOTCH1 EGF-like calcium-binding domain (domain) [591-628]
- IPR050778: Cueball/EGF/LRP/Nidogen (family) [740-1337]
- IPR024731: NELL2-like, EGF domain (domain) [836-1036]
- IPR000742: EGF-like domain (domain) [284-1037]
- IPR009030: Growth factor receptor cysteine-rich domain superfamily (homologous_superfamily) [940-1338]
- IPR006605: G2 nidogen/fibulin G2F (domain) [322-550]
- IPR003886: NIDO domain (domain) [75-262]
Interaction partners: - Viking, isoform A
- Terribly reduced optic lobes, isoform AT
- Laminin subunit gamma-1
- Laminin subunit beta-1
- Hemolectin, isoform A
- Collagen alpha-1
- beta-galactoside alpha-
- BM-40
- Laminin subunit alpha
- Beta-1,3-galactosyltransferase brn
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097367 (carbohydrate derivative binding), GO:1901681 (sulfur compound binding), GO:0005515 (protein binding), GO:0043395 (heparan sulfate proteoglycan binding), GO:0043394 (proteoglycan binding)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0007155 (cell adhesion), GO:0071840 (cellular component organization or biogenesis), GO:0032989 (cellular component morphogenesis), GO:0000902 (cell morphogenesis), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0016358 (dendrite development), GO:0048731 (system development), GO:0016043 (cellular component organization), GO:0031589 (cell-substrate adhesion), GO:0022008 (neurogenesis), GO:0000904 (cell morphogenesis involved in differentiation), GO:0030030 (cell projection organization), GO:0007399 (nervous system development), GO:0032990 (cell part morphogenesis), GO:0048813 (dendrite morphogenesis), GO:0048858 (cell projection morphogenesis), GO:0030182 (neuron differentiation), GO:0048666 (neuron development), GO:0048667 (cell morphogenesis involved in neuron differentiation), GO:0031175 (neuron projection development), GO:0048699 (generation of neurons), GO:0120039 (plasma membrane bounded cell projection morphogenesis), GO:0120036 (plasma membrane bounded cell projection organization), GO:0048812 (neuron projection morphogenesis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0009986 (cell surface)