Protein: Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase (EC 2.3.1.-) (Anthocyanin 5-aromatic acyltransferase-like protein) (Anthocyanin 5-glucoside malonyltransferase) (At5MaT) (Q9LJB4)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Catalyzes the malonylation of the 5-O-glucose residue of anthocyanins, using malonyl-CoA as the malonyl donor. Acts only on anthocyanin substrates containing a 5-O-glucosyl moiety. Acts on the four native A.thaliana anthocyanins, A3, A7, and to a lesser extent, A6 and A10. Can also use the non-native anthocyanin compounds cyanin (cyanidin 3,5-diglucoside), malvin, pelargonidin 3,5-diglucoside, peonidin 3,5-diglucoside, cyanidin 3-coumaroylglucoside 5-glucoside, delphinidin 3-coumaroylrutinoside 5-glucoside and petunidin 3-coumaroylrutinoside 5-glucoside as substrates. Is the sole enzyme responsible for producing malonylated anthocyanin 5-O-glucosides in A.thaliana. Is not able to catalyze acyl transfer using acetyl-CoA, butyryl-CoA, hexanoyl-CoA, benzoyl-CoA, cinnamoyl-CoA, methylmalonyl-CoA, succinyl-CoA, p-coumaroyl-CoA or caffeoyl-CoA.
Sequence: MVNFNSAVNILEVVQVSPPSSNSLTLPLTYFDLGWLKLHPVDRVLFYHVPELTRSSLISKLKSSLSATLLHYLPLAGRLVWDSIKTKPSIVYSPDDKDAVYLTVAESNGDLSHLSGDEPRPATEFHSLVPELPVSDESARVLAVQVTFFPNQGFSLGVTAHHAVLDGKTTAMFLKAWAHNCKQEQEALPHDLVPSLDRIIVQDPTGLETKLLNRWISASNNKPSLKLFPSKIIGSDILRVTYRLTREDIKKLRERVETESHAKQLRLSTFVITYAYVITCMVKMRGGDPTRFVCVGFASDFRSRLNPPLPPTFFGNCIVGSGDFDVKAEPILEEGEGKGFITAVETLTGWVNGLCPENIEKNMLLPFEAFKRMEPGRQMISVAGSTRLGIYGSDFGWGKPVKVEIVTIDKDASVSLSESGDGSGGVEVGVCLKKDDVERFGSLFSIGLE
InterPro domains: - IPR051504: Plant secondary metabolite acyltransferase (family) [8-448]
- IPR023213: Chloramphenicol acetyltransferase-like domain superfamily (homologous_superfamily) [8-449]
Interaction partners: - UDP-glycosyltransferase 75C1
- Anthocyanidin 3-O-glucoside 2'''-O-xylosyltransferase
- Dihydroflavonol 4-reductase
- Glutathione S-transferase F12
- Flavonoid 3'-monooxygenase
- Serine carboxypeptidase-like 10
- Transcription factor TT8
- Leucoanthocyanidin dioxygenase
- NAC domain-containing protein 6
- Naringenin,2-oxoglutarate 3-dioxygenase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016746 (acyltransferase activity), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups), GO:0008374 (O-acyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0042440 (pigment metabolic process), GO:0009058 (biosynthetic process), GO:0071704 (organic substance metabolic process), GO:0046283 (anthocyanin-containing compound metabolic process), GO:0009812 (flavonoid metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0046148 (pigment biosynthetic process), GO:0009718 (anthocyanin-containing compound biosynthetic process), GO:0009813 (flavonoid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0043231 (intracellular membrane-bounded organelle)