Protein: Hsk1-interacting molecule 1 (DNA repair protein rad35) (O59836)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Activates hsk1 kinase and is essential for G1/S transition. Has a role in S-phase checkpoint control induced by replication fork blocks after nucleotide deprivation and DNA damage.
Subcellular location: Nucleus {ECO:0000269|PubMed:10409743}.
Sequence: MNLGRCPLAPRSANIVLPKHDAVSKQKEYRIEEKTNEAQREEIITWKDNREDEGEVKTDFEVVNNENIITTTPKHQTVITPKSYRKSVKRIKHDAPQNEDIPVMKGLAPINADTESKAESMAAGKVLGSKNSSQKARLQEWKRQYKKAFPHFRFYLDGCDPSVAHRVKKQIQQLGGHVETFFSGNVTHVATVRAIQDVSVKYAKQDVITKARQLNMKIWSMEKLCNRVLKTLMENDQCTTNAPTKQGNDLSYLLYVEKVQGTNERDLSVPRQDFVHFRGPYLYVHDIANIYKPILLREWQKPLPDRDVPWPTFRATSIGRCPFVPETKYRLSTSKSLVAKNDQQLLQRQSQEPSLILRANSMKASLPDISNTGISGMNTNTTYNTNINNTPQTAISGITQDTSPSIRTNCHHCLDDGMQASGIVQSNLTSAAMSNNSAIRSGSAASVPVVTINGRDIAELKKRIIQQKSGMIGKDYSYKAMLHNTSQRKIRVDAKPGYCENCREKFDNFESHIRSSRHRRFAENNDNFKDLDELFALVQRPLRPD
InterPro domains: - IPR006572: Zinc finger, DBF-type (domain) [492-541]
- IPR055116: DBF4, BRCT domain (domain) [172-232]
- IPR038545: DBF-type zinc finger superfamily (homologous_superfamily) [497-537]
- IPR013939: Regulatory subunit Dfp1/Him1, central region (domain) [248-324]
- IPR051590: DNA Replication Regulatory Kinase Subunit (family) [126-534]
- IPR001357: BRCT domain (domain) [146-215]
- IPR036420: BRCT domain superfamily (homologous_superfamily) [159-233]
Interaction partners: - Cell cycle serine/threonine-protein kinase hsk1
- DNA replication licensing factor mcm4
- DNA replication licensing factor mcm2
- DNA replication licensing factor mcm5
- DNA replication licensing factor mcm6
- DNA replication licensing factor mcm3
- DNA replication licensing factor mcm7
- Cell cycle protein kinase spo4
- Serine/threonine-protein kinase cds1
- DNA replication licensing factor mcm10
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0005488 (binding), GO:0030234 (enzyme regulator activity), GO:0140677 (molecular function activator activity), GO:1901363 (heterocyclic compound binding), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0019207 (kinase regulator activity), GO:0008047 (enzyme activator activity), GO:0019209 (kinase activator activity), GO:0019887 (protein kinase regulator activity), GO:0030295 (protein kinase activator activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0022414 (reproductive process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0000003 (reproduction), GO:0019953 (sexual reproduction), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0043934 (sporulation), GO:0009653 (anatomical structure morphogenesis), GO:0044237 (cellular metabolic process), GO:0032505 (reproduction of a single-celled organism), GO:0051321 (meiotic cell cycle), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:0022413 (reproductive process in single-celled organism), GO:0071840 (cellular component organization or biogenesis), GO:1903046 (meiotic cell cycle process), GO:0071704 (organic substance metabolic process), GO:0003006 (developmental process involved in reproduction), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0044238 (primary metabolic process), GO:0032989 (cellular component morphogenesis), GO:0030437 (ascospore formation), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0046483 (heterocycle metabolic process), GO:0044786 (cell cycle DNA replication), GO:0030435 (sporulation resulting in formation of a cellular spore), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0034293 (sexual sporulation), GO:0043935 (sexual sporulation resulting in formation of a cellular spore), GO:0090304 (nucleic acid metabolic process), GO:0033260 (nuclear DNA replication), GO:0006259 (DNA metabolic process), GO:0006996 (organelle organization), GO:0007531 (mating type determination), GO:0006260 (DNA replication), GO:0006270 (DNA replication initiation), GO:0006261 (DNA-templated DNA replication)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)