Protein: 1,4-alpha-glucan branching enzyme TTHA1902 (EC 2.4.1.18) (1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase) (Alpha-(1->4)-glucan branching enzyme) (Branching enzyme) (BE) (Q5SH28)
Organism: Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
Function: Catalyzes the formation of branch points in alpha-glucans by cleavage of an alpha-1,4 glycosidic bond and subsequent transfer of the cleaved-off oligosaccharide to a new alpha-1,6 position. The branch chain-length distribution of the reaction products shows degree of polymerization (DP) of 3 to 13, with two local maxima at DP 7 and DP 11. Exhibits an alpha-retaining catalytic mechanism. Is involved in glycogen biosynthesis. Shows a secondary activity, i.e. the hydrolysis of the substrate, being 4% of the total activity. Can use amylose as substrate but not alpha-1,4-linked oligosaccharides of 2-7 glucose residues, beta-cyclodextrin, 6-O-glucosyl-beta-cyclodextrin and 6-O-maltosyl-beta-cyclodextrin. Is not able to branch amylopectin further, it only hydrolyzes amylopectin. Thus, displays preference for linear and long substrates (amylose) over branched structures (amylopectin).
Sequence: MARFALVLHAHLPYVRAHGMWPFGEETLYEAMAETYLPLIRVLERLRAEGVEAPFTLGITPILAEQLADARIKEGFWAYAKDRLERAQGDYQRYRGTALEASARHQVAFWELTLDHFQRLSGDLVAAFRKAEEGGQVELITSNATHGYSPLLGYDEALWAQIKTGVSTYRRHFAKDPTGFWLPEMAYRPKGPWKPPVEGPPEGVRPGVDELLMRAGIRYTFVDAHLVQGGEPLSPYGEAALGPVESQEATYHVHELESGLRVLARNPETTLQVWSADYGYPGEGLYREFHRKDPLSGLHHWRVTHRKADLAEKAPYDPEAAFAKTEEHARHFVGLLERLAGRHPEGVILSPYDAELFGHWWYEGVAWLEAVLRLLAQNPKVRPVTAREAVQGPAVRTALPEGSWGRGGDHRVWLNEKTLDYWEKVYRAEGAMREAARRGVLPEGVLRQAMRELLLLEASDWPFLMETGQAEAYARERYEEHARAFFHLLKGASPEELRALEERDNPFPEADPRLYLFREA
InterPro domains: - IPR015293: 1,4-alpha-glucan branching enzyme, C-terminal (domain) [426-515]
- IPR011330: Glycoside hydrolase/deacetylase, beta/alpha-barrel (homologous_superfamily) [2-413]
- IPR040042: 1,4-alpha-glucan branching enzyme MT3115-like (family) [3-511]
- IPR037090: Glycoside hydrolase families 57, central domain (homologous_superfamily) [414-520]
- IPR048145: 1,4-alpha-glucan branching enzyme TTHA1902 (family) [3-516]
- IPR004300: Glycoside hydrolase family 57, N-terminal domain (domain) [5-391]
- IPR028995: Glycoside hydrolase families 57/38, central domain superfamily (homologous_superfamily) [413-517]
- IPR027291: Glycoside hydrolase 38, N-terminal domain superfamily (homologous_superfamily) [1-413]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016757 (glycosyltransferase activity), GO:0016758 (hexosyltransferase activity), GO:0000030 (mannosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0070085 (glycosylation), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0043413 (macromolecule glycosylation), GO:1901135 (carbohydrate derivative metabolic process), GO:0036211 (protein modification process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0006486 (protein glycosylation), GO:0043412 (macromolecule modification), GO:0009100 (glycoprotein metabolic process), GO:0043436 (oxoacid metabolic process), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0006487 (protein N-linked glycosylation), GO:0009101 (glycoprotein biosynthetic process), GO:0046394 (carboxylic acid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)