Protein: Transcription factor E2-alpha (Immunoglobulin-enhancer-binding factor E12/E47) (Transcription factor 3) (TCF-3) (Transcription regulator Pan) (P98180)
Organism: Mesocricetus auratus (Golden hamster)
Function: Transcriptional regulator. Involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition (By similarity). Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation (By similarity). Together with TCF15, required for the mesenchymal to epithelial transition (By similarity). Dimers bind DNA on E-box motifs: 5'-CANNTG-3' (By similarity). Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer (By similarity). Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region (By similarity).
Subcellular location: Nucleus {ECO:0000250|UniProtKB:P15806}.
Sequence: MMNQSQRMAPVGSDKELSDLLDFSMMFPLPVANGKGRPASLAGTQFAGSGLEDRPSSGSWGNSDQNSSSFDPSRTYSEGAHFSESHNSLPSSTFLGPGLGGKSSERSAYATFGRDTSVSALTQAGFLPGELGLSSPGPLSPSGVKSGSQYYPSYPSNPRRRAADSGLDTQSKKVRKVPPGLPSSVYPSSSGDSYGRDAAAYPSAKTPGSAYPSPFYVADGSLHPSAELWSPPSQAGFGPMLGDGSSPLPLAPGSSSVGSGTFGGLQQQERMSYQLHGSEVNGTLPAVSSFSAAPGTYGGASGHTPPVSGADSLMGTRGTTASSSGDALGKALASIYSPDHSSNNFSPSPSTPVGSPQGLPGTSQWPRAGAPSALSPTYDGGLHGLSKMEDRLDEAIHVLRSHAVGTASDLHGLLPGHGALTTSFPGPVPLGGRHAGLVGGGHPEDGLTSGTSLLHTHASLPSQASSLPDLSQRPPDSYGGLGRAGAPAGASEIKREEKDDEESTSVADAEEDKKDLKAPRTRTSPDEDEDDLLPPEQKAEREKERRVANNARERLRVRDINEAFKELGRMCQLHLSSEKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSGVVGDPQLALSAAHPGLGEAHNPPGHL
InterPro domains: - IPR011598: Myc-type, basic helix-loop-helix (bHLH) domain (domain) [544-603]
- IPR036638: Helix-loop-helix DNA-binding domain superfamily (homologous_superfamily) [531-611]
- IPR051098: Neuronal Differentiation E-box Binding Transcription Factors (family) [6-648]
Interaction partners: - Catenin beta-1
- T-cell acute lymphocytic leukemia protein 1
- DNA-binding protein inhibitor ID-1 isoform X2
- Myogenic factor
- Rhombotin-2
- Myogenin
- LIM domain-binding protein 1 isoform X1
- Achaete-scute homolog 2
- LIM domain-binding protein 2 isoform X1
- Junction plakoglobin
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:0003700 (DNA-binding transcription factor activity), GO:0005515 (protein binding), GO:0008134 (transcription factor binding), GO:0000981 (DNA-binding transcription factor activity, RNA polymerase II-specific), GO:0140297 (DNA-binding transcription factor binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0048522 (positive regulation of cellular process), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010628 (positive regulation of gene expression), GO:0051252 (regulation of RNA metabolic process), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0010468 (regulation of gene expression), GO:1902680 (positive regulation of RNA biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0005667 (transcription regulator complex)