Protein: V-type proton ATPase subunit E (V-ATPase subunit E) (Q95X44)
Organism: Caenorhabditis elegans
Function: Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (By similarity). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). Regulates pH homeostasis in the intestine. Probably by regulating cytoplasmic pH, required for cell survival in the intestine and hypodermis. Involved in receptor-mediated endocytosis. Involved in embryogenesis and larval development.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:12853134}. Apical cell membrane {ECO:0000269|PubMed:12853134, ECO:0000269|PubMed:16785323}; Peripheral membrane protein {ECO:0000305}. Note=Localizes in clusters in the cytoplasm of the excretory canal (PubMed:12853134). Colocalizes with vha-5 to stacked sheets of the apical cell membrane of syncytial hypodermal cells (PubMed:12853134, PubMed:16785323). {ECO:0000269|PubMed:12853134, ECO:0000269|PubMed:16785323}.
Sequence: MGISDNDVQKQLRHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQQQRQKIMEFFEKKEKQVELQRKIQASNSLNAGRLRCLKAREDHIGAVLDEARSNLSRISGDAARYPAILKGLVMQGLLQLLEKEVVLRCREKDLRLVEQLLPECLDGLQKEWGSTTKVVLDKQNFLPSESAGGVELSARAGKIKVSSTLESRLELIANQIVPQVRTALFGPNPNRSFFD
InterPro domains: - IPR038495: V-type ATPase subunit E, C-terminal domain superfamily (homologous_superfamily) [93-226]
- IPR002842: V-type ATPase subunit E (family) [4-219]
Interaction partners: - V-type proton ATPase catalytic subunit A
- V-type proton ATPase subunit D
- Probable V-type proton ATPase subunit F
- Probable V-type proton ATPase subunit G
- V-type proton ATPase subunit B 1
- V-type proton ATPase subunit C
- Probable V-type proton ATPase subunit H 2
- V-type proton ATPase subunit
- V-type proton ATPase subunit B 2
- V-type proton ATPase 16 kDa proteolipid subunit c 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005215 (transporter activity), GO:0140657 (ATP-dependent activity), GO:0042626 (ATPase-coupled transmembrane transporter activity), GO:0022857 (transmembrane transporter activity), GO:0042625 (ATPase-coupled ion transmembrane transporter activity), GO:0015075 (monoatomic ion transmembrane transporter activity), GO:0015318 (inorganic molecular entity transmembrane transporter activity), GO:0019829 (ATPase-coupled monoatomic cation transmembrane transporter activity), GO:0022804 (active transmembrane transporter activity), GO:0015399 (primary active transmembrane transporter activity), GO:0022890 (inorganic cation transmembrane transporter activity), GO:0022853 (active monoatomic ion transmembrane transporter activity), GO:0044769 (ATPase activity, coupled to transmembrane movement of ions, rotational mechanism), GO:0008324 (monoatomic cation transmembrane transporter activity), GO:0046961 (proton-transporting ATPase activity, rotational mechanism), GO:0015078 (proton transmembrane transporter activity), GO:0009678 (pyrophosphate hydrolysis-driven proton transmembrane transporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0032501 (multicellular organismal process), GO:2000241 (regulation of reproductive process), GO:0051239 (regulation of multicellular organismal process), GO:0008340 (determination of adult lifespan), GO:0050795 (regulation of behavior), GO:0046662 (regulation of oviposition)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0030141 (secretory granule), GO:0043227 (membrane-bounded organelle), GO:0001669 (acrosomal vesicle), GO:0031982 (vesicle), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0097708 (intracellular vesicle)