Protein: Methionine synthase (EC 2.1.1.13) (5-methyltetrahydrofolate--homocysteine methyltransferase) (Methionine synthase, vitamin-B12-dependent) (MS) (P13009)
Organism: Escherichia coli (strain K12)
Function: Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.
Sequence: MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFADWPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAEINFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLVAAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDASGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAGLPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLPEIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVENGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQGKGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKILTEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVSFSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRRDDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEARQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASKEQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNADLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVYVQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDFAFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVEAQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLRQQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMVKALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHTEKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYARRKGMSVTEVERWLAPNLGYDAD
InterPro domains: - IPR006158: Cobalamin (vitamin B12)-binding domain (domain) [746-881]
- IPR003726: Homocysteine-binding domain (domain) [5-325]
- IPR037010: Vitamin B12-dependent methionine synthase, activation domain superfamily (homologous_superfamily) [902-1226]
- IPR050554: Methionine Synthase/Corrinoid (family) [6-1224]
- IPR011822: Cobalamin-dependent methionine synthase (family) [1-1195]
- IPR003759: Cobalamin (vitamin B12)-binding module, cap domain (domain) [650-744]
- IPR004223: Vitamin B12-dependent methionine synthase, activation domain (domain) [897-1227]
- IPR036594: Methionine synthase domain (homologous_superfamily) [644-740]
- IPR036724: Cobalamin-binding domain superfamily (homologous_superfamily) [743-894]
- IPR000489: Pterin-binding domain (domain) [356-617]
- IPR033706: Methionine synthase, B12-binding domain (domain) [659-871]
- IPR036589: Homocysteine-binding domain superfamily (homologous_superfamily) [1-399]
- IPR011005: Dihydropteroate synthase-like superfamily (homologous_superfamily) [346-643]
Interaction partners: - 5,10-methylenetetrahydrofolate reductase
- 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
- Cystathionine gamma-synthase
- S-adenosylmethionine synthase
- Cystathionine beta-lyase MetC
- Serine hydroxymethyltransferase
- Aminomethyltransferase
- Bifunctional aspartokinase/homoserine dehydrogenase 2
- Bifunctional aspartokinase/homoserine dehydrogenase 1
- S-ribosylhomocysteine lyase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0008168 (methyltransferase activity), GO:0008172 (S-methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0006082 (organic acid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0006766 (vitamin metabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006767 (water-soluble vitamin metabolic process), GO:0000096 (sulfur amino acid metabolic process), GO:0044272 (sulfur compound biosynthetic process), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0033013 (tetrapyrrole metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0009235 (cobalamin metabolic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0009066 (aspartate family amino acid metabolic process), GO:0000097 (sulfur amino acid biosynthetic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0006555 (methionine metabolic process), GO:0009086 (methionine biosynthetic process), GO:0009067 (aspartate family amino acid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)