Protein: Nuclear cap-binding protein subunit 2 (20 kDa nuclear cap-binding protein) (NCBP 20 kDa subunit) (CBP20) (Q9V3L6)
Organism: Drosophila melanogaster (Fruit fly)
Function: Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi). The CBC complex is involved in miRNA-mediated RNA interference via its interaction with Ars2 and is required for primary microRNAs (miRNAs) processing. Also involved in innate immunity via the short interfering RNAs (siRNAs) processing machinery by restricting the viral RNA production. In the CBC complex, Cbp20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires Cbp80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure.
Subcellular location: Nucleus {ECO:0000250}.
Sequence: MFASVELSSYRDQHFKGSRSEQERSLRDSCTLYVGNLSFYTTEEQIHELFSRCGDVRVIVMGLDKYKKTPCGFCFVEYYVRSEAEAAMRFVNGTRLDDRLIRVDWDAGFVEGRQYGRGKTGGQVRDEYRTDYDAGRGGYGKLLSQKIAPNTDNR
InterPro domains: - IPR035979: RNA-binding domain superfamily (homologous_superfamily) [22-140]
- IPR012677: Nucleotide-binding alpha-beta plait domain superfamily (homologous_superfamily) [3-146]
- IPR000504: RNA recognition motif domain (domain) [30-108]
- IPR034148: NCBP2, RNA recognition motif (domain) [32-109]
- IPR027157: Nuclear cap-binding protein subunit 2 (family) [4-147]
Interaction partners: - Nuclear cap-binding protein subunit 1
- Serrate RNA effector molecule homolog
- U1 small nuclear ribonucleoprotein 70 kDa
- Probable small nuclear ribonucleoprotein G
- LD21442p
- Small nuclear ribonucleoprotein F
- Probable small nuclear ribonucleoprotein Sm D2
- Phosphorylated adapter RNA export protein
- U1 small nuclear ribonucleoprotein C
- THO complex subunit 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0000339 (RNA cap binding), GO:0000340 (RNA 7-methylguanosine cap binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0044237 (cellular metabolic process), GO:0048522 (positive regulation of cellular process), GO:0032879 (regulation of localization), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0051234 (establishment of localization), GO:0071704 (organic substance metabolic process), GO:0051050 (positive regulation of transport), GO:0044238 (primary metabolic process), GO:0033036 (macromolecule localization), GO:0051049 (regulation of transport), GO:1901360 (organic cyclic compound metabolic process), GO:0046907 (intracellular transport), GO:0032388 (positive regulation of intracellular transport), GO:0006139 (nucleobase-containing compound metabolic process), GO:0006403 (RNA localization), GO:0046483 (heterocycle metabolic process), GO:0060341 (regulation of cellular localization), GO:0006810 (transport), GO:0051649 (establishment of localization in cell), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0051236 (establishment of RNA localization), GO:0043170 (macromolecule metabolic process), GO:0032239 (regulation of nucleobase-containing compound transport), GO:0050658 (RNA transport), GO:0071705 (nitrogen compound transport), GO:0090304 (nucleic acid metabolic process), GO:0046824 (positive regulation of nucleocytoplasmic transport), GO:0071702 (organic substance transport), GO:0051169 (nuclear transport), GO:0010467 (gene expression), GO:0032386 (regulation of intracellular transport), GO:0015931 (nucleobase-containing compound transport), GO:0006913 (nucleocytoplasmic transport), GO:0016070 (RNA metabolic process), GO:0046831 (regulation of RNA export from nucleus), GO:0006406 (mRNA export from nucleus), GO:0006405 (RNA export from nucleus), GO:0006396 (RNA processing), GO:0046822 (regulation of nucleocytoplasmic transport), GO:0008380 (RNA splicing), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0051168 (nuclear export), GO:0050657 (nucleic acid transport), GO:0000398 (mRNA splicing, via spliceosome), GO:0000375 (RNA splicing, via transesterification reactions), GO:0000381 (regulation of alternative mRNA splicing, via spliceosome), GO:0000377 (RNA splicing, via transesterification reactions with bulged adenosine as nucleophile)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0034518 (RNA cap binding complex), GO:0140535 (intracellular protein-containing complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)