Protein: dITP/XTP pyrophosphatase (EC 3.6.1.66) (Non-canonical purine NTP pyrophosphatase) (Non-standard purine NTP pyrophosphatase) (Nucleoside-triphosphate diphosphatase) (Nucleoside-triphosphate pyrophosphatase) (NTPase) (O28046)
Organism: Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)
Function: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides xanthosine triphosphate (XTP), deoxyinosine triphosphate (dITP) and ITP. Probably functions as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions. Shows very low activity on dGTP or dUTP, and has nearly no activity toward the canonical nucleotides ATP, CTP, and TTP.
Sequence: MYFITSNEGKFREVREMASKYGIEIEWLKMEYIEPQGSSLEEIARLSAEMLAEKVEGEFVIEDSGLFVEALKGFPGPYSSYVFKTIGNEGILKLMEGVENRKAYFMAVVAYFDGKEVRTFTGKVEGEISREMRGTQGFGYDPIFLYGNKTFAEMATEEKNQVSHRRKAFEEFFRWLKENKI
InterPro domains: - IPR029001: Inosine triphosphate pyrophosphatase-like (homologous_superfamily) [1-181]
- IPR020922: dITP/XTP pyrophosphatase (family) [1-178]
- IPR002637: RdgB/HAM1 (family) [1-180]
Interaction partners: - Nucleoside diphosphate kinase
- GMP synthase subunit B
- GMP synthase subunit A
- 5'-nucleotidase SurE
- Small ribosomal subunit protein eS1
- Exosome complex component Rrp41
- Dihydropteroate synthase
- Adenylosuccinate synthetase
- Phosphoesterase
- Phosphoesterase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0042802 (identical protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0044248 (cellular catabolic process), GO:0019637 (organophosphate metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0006793 (phosphorus metabolic process), GO:0034655 (nucleobase-containing compound catabolic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0019439 (aromatic compound catabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0046700 (heterocycle catabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0046434 (organophosphate catabolic process), GO:0009141 (nucleoside triphosphate metabolic process), GO:1901292 (nucleoside phosphate catabolic process), GO:0009144 (purine nucleoside triphosphate metabolic process), GO:0009143 (nucleoside triphosphate catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex)