Protein: Forkhead box protein N4 (Q8K3Q3)
Organism: Mus musculus (Mouse)
Function: Transcription factor essential for neural and some non-neural tissues development, such as retina and lung respectively. Binds to an 11-bp consensus sequence containing the invariant tetranucleotide 5'-ACGC-3'. During development of the central nervous system, is required to specify the amacrine and horizontal cell fates from multipotent retinal progenitors while suppressing the alternative photoreceptor cell fates through activating DLL4-NOTCH signaling. Also acts synergistically with ASCL1/MASH1 to activate DLL4-NOTCH signaling and drive commitment of p2 progenitors to the V2b interneuron fates during spinal cord neurogenesis. In development of non-neural tissues, plays an essential role in the specification of the atrioventricular canal and is indirectly required for patterning the distal airway during lung development.
Subcellular location: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00089, ECO:0000269|PubMed:22323600}.
Sequence: MIESGIWSRMSEMIRSSGHSHHCSPQEYRFLPPVGDDDLPGDLQSLSWLTAVDVPRLQQMANGRIDLGSSGVTHPHPGALAGTADLHVGAAPRPLLRRSQTAVVPRGVLGLSPIGNHRASAEQMNQFPAGGQASSGLQEMPQLYSPATQIPFPLPLGSQQCPPAGLYGSPFSARPSYPQAHGAMHASQEPHPKHYPKPIYSYSCLIAMALKNSKTGSLPVSEIYSFMKEHFPYFKTAPDGWKNSVRHNLSLNKCFEKVETKSSGSSRKGCLWALNLARIDKMEEEMHKWKRKDLAAIHRSMANPEELDKLISDRPESCRRPGKRGEPKAPMLTHATTVAMAHSCLAISQLPPKPLMTLSLQSVPLHHQLQPQAHLAPDSPAPAQTPPLHALPSLSPGPLPQPAMGRVPGDFLNINSDMNTEVDALDPSIMDFALQGNLWEEMKEDSFSLDTLEAFGDSPLGCDLGAPSLTPVSGNSDQSFPDVQVTGLYAAYSTAADGVAPSAANSAQYLGTPGNKPIALL
InterPro domains: - IPR036390: Winged helix DNA-binding domain superfamily (homologous_superfamily) [197-292]
- IPR030456: Fork head domain conserved site 2 (conserved_site) [241-247]
- IPR049624: Forkhead box protein N1/4 (family) [144-520]
- IPR001766: Fork head domain (domain) [195-293]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [183-293]
Interaction partners: - Pancreas transcription factor 1 subunit alpha
- Visual system homeobox 1
- Transcription factor Atoh7
- Visual system homeobox 2
- Neurogenic differentiation factor 4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:0097159 (organic cyclic compound binding), GO:0003700 (DNA-binding transcription factor activity), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0000981 (DNA-binding transcription factor activity, RNA polymerase II-specific), GO:0003677 (DNA binding), GO:0043565 (sequence-specific DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0042592 (homeostatic process), GO:0002376 (immune system process), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0001775 (cell activation), GO:0009653 (anatomical structure morphogenesis), GO:0048870 (cell motility), GO:0045321 (leukocyte activation), GO:0019222 (regulation of metabolic process), GO:0051240 (positive regulation of multicellular organismal process), GO:0042303 (molting cycle), GO:0002682 (regulation of immune system process), GO:0051239 (regulation of multicellular organismal process), GO:0048871 (multicellular organismal-level homeostasis), GO:0050793 (regulation of developmental process), GO:0048522 (positive regulation of cellular process), GO:0002520 (immune system development), GO:0022404 (molting cycle process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0001776 (leukocyte homeostasis), GO:0051094 (positive regulation of developmental process), GO:0042634 (regulation of hair cycle), GO:0002260 (lymphocyte homeostasis), GO:0048736 (appendage development), GO:0048729 (tissue morphogenesis), GO:0042633 (hair cycle), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0001568 (blood vessel development), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0060255 (regulation of macromolecule metabolic process), GO:0002694 (regulation of leukocyte activation), GO:1903706 (regulation of hemopoiesis), GO:0046649 (lymphocyte activation), GO:0009891 (positive regulation of biosynthetic process), GO:0048872 (homeostasis of number of cells), GO:0035295 (tube development), GO:0045165 (cell fate commitment), GO:2000026 (regulation of multicellular organismal development), GO:0009888 (tissue development), GO:0031325 (positive regulation of cellular metabolic process), GO:0001942 (hair follicle development), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0045595 (regulation of cell differentiation), GO:0031323 (regulation of cellular metabolic process), GO:0022405 (hair cycle process), GO:0080090 (regulation of primary metabolic process), GO:0048534 (hematopoietic or lymphoid organ development), GO:0050865 (regulation of cell activation), GO:0016477 (cell migration), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0035239 (tube morphogenesis), GO:0030097 (hemopoiesis), GO:0002521 (leukocyte differentiation), GO:0002360 (T cell lineage commitment), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0001944 (vasculature development), GO:0060284 (regulation of cell development), GO:0048732 (gland development), GO:0002009 (morphogenesis of an epithelium), GO:0043588 (skin development), GO:0048538 (thymus development), GO:0010468 (regulation of gene expression), GO:0030098 (lymphocyte differentiation), GO:0030855 (epithelial cell differentiation), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0048514 (blood vessel morphogenesis), GO:0008544 (epidermis development), GO:0072359 (circulatory system development), GO:0031326 (regulation of cellular biosynthetic process), GO:0043029 (T cell homeostasis), GO:0042110 (T cell activation), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:1902105 (regulation of leukocyte differentiation), GO:0060173 (limb development), GO:0051252 (regulation of RNA metabolic process), GO:0060429 (epithelium development), GO:0051797 (regulation of hair follicle development), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0051249 (regulation of lymphocyte activation), GO:0045619 (regulation of lymphocyte differentiation), GO:0050863 (regulation of T cell activation), GO:0030217 (T cell differentiation), GO:2001141 (regulation of RNA biosynthetic process), GO:0098773 (skin epidermis development), GO:0006355 (regulation of DNA-templated transcription), GO:0030216 (keratinocyte differentiation), GO:1903131 (mononuclear cell differentiation), GO:1902680 (positive regulation of RNA biosynthetic process), GO:0009913 (epidermal cell differentiation), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0045580 (regulation of T cell differentiation), GO:0045944 (positive regulation of transcription by RNA polymerase II), GO:0033081 (regulation of T cell differentiation in thymus)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)