Protein: Mannan endo-1,4-beta-mannosidase (EC 3.2.1.78) (Beta-mannanase) (Mannanase 26A) (Man26A) (Mannanase A) (ManA) (A1A278)
Organism: Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)
Function: Beta-mannanase likely involved in the utilization of carbohydrates in the human gut. Catalyzes the hydrolysis of different beta-1,4-linked mannans, such as ivory nut mannan, konjac glucomannan, as well as carob and guar gum galactomannans, to a mixture of oligosaccharides. The dominant product from ivory nut mannan is found to be mannotriose; mannobiose and mannotetraose are produced to a lesser extent. Does not hydrolyze mannobiose, and hydrolyzes mannotriose at a significantly lower rate than the longer oligosaccharides.
Subcellular location: Secreted, cell wall {ECO:0000305}; Peptidoglycan-anchor {ECO:0000305}. Note=Is likely to be cell attached, either by the sortase mechanism (LPXTG motif) or via a C-terminal transmembrane helix. {ECO:0000269|PubMed:23064345}.
Sequence: MKTTVTKLLATVAAASTIFGMSTLPAFAAEGKSASNGNSVNISDVNATAETRALFDKLKNSGKGDLRFGQQHATDENISSSASQGDVYETTGKYPAVFGWDAGLALRGAEKPGSGADKNANAKALAQNITDADSKGAIVTLSAHWCNPGTGKDFNDTTAVASELLPGGKYSGTFNKELDAIAATAQRAKRSDGTLIPIIFRPLHENNGSWFWWGATHASASEYKELYRYIVDYLRDVKDVHNLLYAYSPGGVFNGDSTDYLATYPGDQWVDVLGYDEYDSDDSADDSSAWINTVVKDMKMVSDQASQRGKIVALTEFGRSGDRKFKESGTGDKDTKFFSELAEALAENVPSTAYMMTWANFGGGGDNFQAYTSWKGSDGEADFKAFADSNKNLMASKDNVDYSNAPAAAMQNGSARIVTPVDGNRVTDTKVVVRVKTEGVKYSDLDLNSAIVTTDRGQNVKLKYSCNGYFTGILDLNAAGINLDQSKLTLTPQVKTKDGKTLAAADGNGSVTVKLGAKPEQTVDNVEDFDSYDNEAELQSVYSPSHSTKSNLTLVDSPEDNGTKAGNIHYDFVSYPEYNGFQRSHTPKQDWSGFSKLNMFLKADGSDHKFVVQVNAGGVTFEAYPKIDGTDGHVVSLNFGDADGNGGDFAPASWDTAHAGMKLSQKLLSKVGSFALYINDNGGNRPKSGDLTLDSIKLDGKRDAYAPNTNPTPGNTAKAQSVDDFSGYSDDAAAQSAWGNRGHTEVLSLDEGPTDGSKALRFKYDFSNGGWYDVAKYLDGANWSGESVLAFQVKGDGSGNAIGLQIGTSDGKYFLASVKLDFTGWKQIEIPLVDNANLTQSWPEDANKDNPMTEDDLASIKELVFASQQWNSESDGLDSSIADIKVEPAENTSNEQTPKDESKTEVKADKEQEQSEDTSADVTAQDPATCPISDEDSKGSTGNTTVTVKPTPDTKEPADNTGKDGLSRTGSNIISAIAAVAVLLLGGCAVLIARKRKGGDIE
InterPro domains: - IPR017853: Glycoside hydrolase superfamily (homologous_superfamily) [40-396]
- IPR013783: Immunoglobulin-like fold (homologous_superfamily) [418-513]
- IPR000805: Glycoside hydrolase family 26 (family) [43-358]
- IPR019931: LPXTG cell wall anchor domain (domain) [966-1002]
- IPR005087: Carbohydrate binding module family 11 (domain) [708-882]
- IPR008979: Galactose-binding-like domain superfamily (homologous_superfamily) [526-868]
- IPR022790: Glycosyl hydrolase family 26 domain (domain) [44-396]
Interaction partners: - Secreted protein
- Reducing end xylose-releasing exo-oligoxylanase
- Sialic acid-specific 9-O-acetylesterase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0052689 (carboxylic ester hydrolase activity), GO:0034338 (short-chain carboxylesterase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0005975 (carbohydrate metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0044248 (cellular catabolic process), GO:0044275 (cellular carbohydrate catabolic process), GO:0009057 (macromolecule catabolic process), GO:0016052 (carbohydrate catabolic process), GO:0044264 (cellular polysaccharide metabolic process), GO:0005976 (polysaccharide metabolic process), GO:0000272 (polysaccharide catabolic process), GO:0044042 (glucan metabolic process), GO:0044247 (cellular polysaccharide catabolic process), GO:0006073 (cellular glucan metabolic process), GO:0051275 (beta-glucan catabolic process), GO:0009251 (glucan catabolic process), GO:0051273 (beta-glucan metabolic process), GO:0030243 (cellulose metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005576 (extracellular region)