Protein: Tyrosine-protein kinase sid-3 (EC 2.7.10.2) (Systemic RNA interference defective protein 3) (Systemic RNAi enabling protein) (Q10925)
Organism: Caenorhabditis elegans
Function: Tyrosine-protein kinase which plays a role in RNA-mediated gene silencing by mediating import of double-stranded RNA (dsRNA) into cells. Not required for import of ingested dsRNA into intestinal cells but involved in subsequent export from intestinal cells to internal tissues.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:22912399}. Note=Displays diffuse localization in cytoplasm and cytoplasmic foci, and also in a punctate pattern.
Sequence: MASTSGALVDDNVLEVLRKAQLDAFISQFVFLFNVRRFDHFSHVRDKDMLEIGMQQVQIRQLREQILKMSREMWNRSDPKQVYIQADQSMPAQNSIDEKALIPNEQIKLYELIGEGSFAVVKRGTWTQSNGTHVNVAVKILRDISPNIMDDLRVEASHLLKLQHPSLIRLYGIVRQPAMMVFELCEGGSLLDRLRDDKKAILLVSRLHDYCMQIAKALQFLESKHCVHRDVAARNILLARDERTVKICDFGLMRALKENEQMYTMAPQKKVPFAWCPPEALRHRKFSHASDVWSYGVTIWEVFTFGEEPWVGCRAIDVLKNIDAGERLEKPKYCSERIYQIMKNCWKFNPAERCKFGAIREDLVAAMFLDAVARETYNSIQPGALQLTKGDEVVVVENTGQDWFGQNKKNQKFGTFPRSVVFAQTNNAVAAATAVTPQKVPTAPTIRIPPSHPPPAPLKPLNNNTKTSLNDRTSKISMPVAGSFIHTGHGDPLGGQSWGNPATIADMYLKNPVNGAPLSSMSSGAEIIASKELLTNGGRSTHQPAAPSPAVMSKIRGLSLDLPEYDDFDRAFDDGFSPSKIELPREFCGNDSVISGGSNSIGLANTYVMEPPKQAFDIRGNRVLPPTNKAPVLIPTNPAPSVISSTASAGITLSTNSSQMFTSQDRHSNMPANLFPELQHRLNQGSSTGNGVRPRPASSIGIQNNDLSMLNPQVNRPFSVVNVPIVQQPANIPCLVPTPAPPAPAHFSQPVSSQRVAQQQQNTLQKALNDELKGNLNKRPTGTTAPPSNGFNAPRADVAPVQQRPISSASIPALQPQPIQHIQKPIQPQQVRIPPSTAPVQKPVQVSAPTHSNVAPTTSSQASADARNPLPPKTSPPVSNTPITVAPVHAAPTTSAPSTSVVTRRPTSTTAQMSDEERRSRIAMDISSALPAPSALLYGSNSTSSLPSAAVSTASSVPSTARDNPVETRPSQPHVTMPPKKSSEPILSSEVLQPTRLPSATTSQAKPVTQPIRHPSPPVATVIPTAVVDKKPVSQNQGSNVPLFNITNSSNGYPQLNGYPNYGNGFQAYGYGMNYHQGYPGYQGYNSYGNGMGQLALTHNAVTSLPPLVPSENRFSGTAQPLGESDIMEFLGTQQRQAGSSSRAVPPASASTSAASGITDLSMADKMEVLYREADFTHKGNCDTMVSQCNGNTEQALKLLKQQHLVDMELAMSTETARQALEARQYDLPAAANMLLG
InterPro domains: - IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [96-368]
- IPR036028: SH3-like domain superfamily (homologous_superfamily) [369-421]
- IPR015116: Cdc42 binding domain-like (domain) [473-522]
- IPR050198: Non-receptor tyrosine kinases involved in cell signaling (family) [79-365]
- IPR001452: SH3 domain (domain) [366-426]
- IPR037085: Cdc42 binding domain-like superfamily (homologous_superfamily) [470-511]
- IPR001245: Serine-threonine/tyrosine-protein kinase, catalytic domain (domain) [107-363]
- IPR049587: TNK(ACK)-like non-receptor tyrosine-protein kinases, SAM domain (domain) [9-70]
- IPR017441: Protein kinase, ATP binding site (binding_site) [113-139]
- IPR000719: Protein kinase domain (domain) [107-369]
- IPR008266: Tyrosine-protein kinase, active site (active_site) [226-238]
- IPR055175: ACK/TNK-like, SAM domain (domain) [14-67]
- IPR020635: Tyrosine-protein kinase, catalytic domain (domain) [107-363]
Interaction partners: - Systemic RNA interference defective protein 5
- Systemic RNA interference defective protein 2
- WH1 domain-containing protein
- Systemic RNA interference defective protein 1
- NCK adaptor protein family
- ENTH domain-containing protein
- Probable clathrin heavy chain 1
- Uncharacterized protein B0280.2
- WH1 domain-containing protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004713 (protein tyrosine kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0050793 (regulation of developmental process), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0071704 (organic substance metabolic process), GO:0051094 (positive regulation of developmental process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0045597 (positive regulation of cell differentiation), GO:1901564 (organonitrogen compound metabolic process), GO:0040028 (regulation of vulval development), GO:0035556 (intracellular signal transduction), GO:0019538 (protein metabolic process), GO:0045595 (regulation of cell differentiation), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0006793 (phosphorus metabolic process), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0060284 (regulation of cell development), GO:0006796 (phosphate-containing compound metabolic process), GO:0007264 (small GTPase mediated signal transduction), GO:0007265 (Ras protein signal transduction), GO:0006468 (protein phosphorylation), GO:0016310 (phosphorylation), GO:0007266 (Rho protein signal transduction)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)