Protein: 6-phospho-alpha-glucosidase (EC 3.2.1.122) (Q9AGA6)
Organism: Klebsiella pneumoniae
Function: Is involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). Hydrolyzes a wide variety of 6-phospho-alpha-D-glucosides including maltose-6'-phosphate, isomaltose-6'-phosphate, maltitol-6-phosphate, trehalose-6-phosphate and the 6'-phosphorylated derivatives of the five linkage-isomeric alpha-D-glucosyl-D-fructoses: trehalulose-6'-phosphate, turanose-6'-phosphate, maltulose-6'-phosphate, leucrose-6'-phosphate, and palatinose-6'-phosphate. However, sucrose-6-phosphate is not a substrate for this enzyme.
Sequence: MKKFSVVIAGGGSTFTPGIVLMLLANQDRFPLRSLKFYDNDGARQETIAEACKVILKEQAPEIEFSYTTDPQAAFTDVDFVMAHIRVGKYPMREQDEKIPLRHGVLGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLRPNAKILNICDMPIGIEGRMAQIVGLKDRKQMRVRYYGLNHFGWWTSIEDLDGNDLMPKLREYVAKYGYVPPSNDPHTEASWNDTFAKAKDVQALDPQTMPNTYLKYYLFPDYVVAHSNPERTRANEVMDHREKNVFSACRAIIAAGKSTAGDLEIDEHASYIVDLATAIAFNTQERMLLIVPNNGAIHNFDADAMVEIPCLVGHNGPEPLTVGDIPHFQKGLMSQQVAVEKLVVDAWEQRSYHKLWQAITLSKTVPSASVAKAILDDLIAANKDYWPELH
InterPro domains: - IPR036291: NAD(P)-binding domain superfamily (homologous_superfamily) [3-164]
- IPR001088: Glycoside hydrolase, family 4 (family) [1-439]
- IPR022616: Glycosyl hydrolase, family 4, C-terminal (domain) [195-415]
- IPR015955: Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal (homologous_superfamily) [167-440]
- IPR019802: Glycoside hydrolase, family 4, conserved site (conserved_site) [138-169]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0043169 (cation binding), GO:0016798 (hydrolase activity, acting on glycosyl bonds), GO:0004553 (hydrolase activity, hydrolyzing O-glycosyl compounds), GO:0070403 (NAD+ binding), GO:0046872 (metal ion binding), GO:0017076 (purine nucleotide binding), GO:0015925 (galactosidase activity), GO:0046914 (transition metal ion binding), GO:0030554 (adenyl nucleotide binding), GO:0030145 (manganese ion binding), GO:0051287 (NAD binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0005975 (carbohydrate metabolic process), GO:1901575 (organic substance catabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0044248 (cellular catabolic process), GO:0044275 (cellular carbohydrate catabolic process), GO:0009311 (oligosaccharide metabolic process), GO:0016052 (carbohydrate catabolic process), GO:0005984 (disaccharide metabolic process), GO:0046352 (disaccharide catabolic process), GO:0009313 (oligosaccharide catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)