Protein: Protein pelota homolog (Q9BRX2)
Organism: Homo sapiens (Human)
Function: Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. In the Pelota-HBS1L complex, PELO recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway. As part of the PINK1-regulated signaling, upon mitochondrial damage is recruited to the ribosome/mRNA-ribonucleoprotein complex associated to mitochondrial outer membrane thereby enabling the recruitment of autophagy receptors and induction of mitophagy
Subcellular location: Cytoplasm
Sequence: MKLVRKNIEKDNAGQVTLVPEEPEDMWHTYNLVQVGDSLRASTIRKVQTESSTGSVGSNRVRTTLTLCVEAIDFDSQACQLRVKGTNIQENEYVKMGAYHTIELEPNRQFTLAKKQWDSVVLERIEQACDPAWSADVAAVVMQEGLAHICLVTPSMTLTRAKVEVNIPRKRKGNCSQHDRALERFYEQVVQAIQRHIHFDVVKCILVASPGFVREQFCDYLFQQAVKTDNKLLLENRSKFLQVHASSGHKYSLKEALCDPTVASRLSDTKAAGEVKALDDFYKMLQHEPDRAFYGLKQVEKANEAMAIDTLLISDELFRHQDVATRSRYVRLVDSVKENAGTVRIFSSLHVSGEQLSQLTGVAAILRFPVPELSDQEGDSSSEED
InterPro domains: - IPR005141: eRF1 domain 2 (domain) [136-268]
- IPR004405: Pelota (family) [1-378]
- IPR005140: eRF1/Pelota-like, N-terminal domain (domain) [1-130]
- IPR058547: Pelota, N-terminal domain (domain) [1-129]
- IPR029064: Ribosomal protein eL30-like superfamily (homologous_superfamily) [262-385]
- IPR038069: Pelota/DOM34, N-terminal domain (homologous_superfamily) [1-132]
- IPR005142: eRF1 domain 3 (domain) [272-370]
- IPR042226: eRF1, domain 2 superfamily (homologous_superfamily) [136-267]
Interaction partners: - HBS1-like protein
- Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein
- Small ribosomal subunit protein RACK1
- ATP-binding cassette sub-family E member 1
- ATP-binding cassette sub-family E member 1
- Small ribosomal subunit protein uS3
- Small ribosomal subunit protein eS6
- Small ribosomal subunit protein uS10
- Small ribosomal subunit protein uS12
- Ubiquitin-ribosomal protein eL40 fusion protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009058 (biosynthetic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0009892 (negative regulation of metabolic process), GO:0044237 (cellular metabolic process), GO:0048522 (positive regulation of cellular process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0044248 (cellular catabolic process), GO:0019538 (protein metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0016043 (cellular component organization), GO:0043603 (amide metabolic process), GO:0080090 (regulation of primary metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0034655 (nucleobase-containing compound catabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0006518 (peptide metabolic process), GO:0034250 (positive regulation of amide metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0006446 (regulation of translational initiation), GO:0019439 (aromatic compound catabolic process), GO:0046700 (heterocycle catabolic process), GO:0043604 (amide biosynthetic process), GO:0010628 (positive regulation of gene expression), GO:0045948 (positive regulation of translational initiation), GO:0010468 (regulation of gene expression), GO:1901566 (organonitrogen compound biosynthetic process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0009057 (macromolecule catabolic process), GO:0006412 (translation), GO:0034248 (regulation of amide metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0044265 (cellular macromolecule catabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:1901361 (organic cyclic compound catabolic process), GO:0051247 (positive regulation of protein metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0006996 (organelle organization), GO:0022411 (cellular component disassembly), GO:0010467 (gene expression), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0010629 (negative regulation of gene expression), GO:0016070 (RNA metabolic process), GO:0043043 (peptide biosynthetic process), GO:0006402 (mRNA catabolic process), GO:0006401 (RNA catabolic process), GO:1903008 (organelle disassembly), GO:0006414 (translational elongation), GO:2000112 (regulation of cellular macromolecule biosynthetic process), GO:0006417 (regulation of translation), GO:0045727 (positive regulation of translation), GO:0010608 (post-transcriptional regulation of gene expression), GO:0000956 (nuclear-transcribed mRNA catabolic process), GO:0016071 (mRNA metabolic process), GO:0034661 (ncRNA catabolic process), GO:0034660 (ncRNA metabolic process), GO:0016075 (rRNA catabolic process), GO:0016072 (rRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)