Protein: Ras-related protein Rab-43 (Ras-related protein Rab-41) (Q86YS6)
Organism: Homo sapiens (Human)
Function: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The low intrinsic GTPase activity of RAB43 is activated by USP6NL. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for the structural integrity of the Golgi complex. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.
Subcellular location: Cytoplasmic vesicle, phagosome {ECO:0000269|PubMed:21255211}. Cytoplasmic vesicle, phagosome membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Golgi apparatus {ECO:0000269|PubMed:17562788, ECO:0000269|PubMed:18664496}. Golgi apparatus, trans-Golgi network membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}. Golgi apparatus, trans-Golgi network {ECO:0000269|PubMed:17562788}. Note=Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211). {ECO:0000269|PubMed:21255211}.
Sequence: MAGPGPGPGDPDEQYDFLFKLVLVGDASVGKTCVVQRFKTGAFSERQGSTIGVDFTMKTLEIQGKRVKLQIWDTAGQERFRTITQSYYRSANGAILAYDITKRSSFLSVPHWIEDVRKYAGSNIVQLLIGNKSDLSELREVSLAEAQSLAEHYDILCAIETSAKDSSNVEEAFLRVATELIMRHGGPLFSEKSPDHIQLNSKDIGEGWGCGC
InterPro domains: - IPR048040: Rab GTPase 19/43 (family) [16-180]
- IPR050209: Rab GTPases family involved in membrane trafficking (family) [15-182]
- IPR005225: Small GTP-binding domain (domain) [19-173]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [10-203]
- IPR001806: Small GTPase (family) [13-212]
Interaction partners: - USP6 N-terminal-like protein
- Trafficking protein particle complex subunit 8
- Trafficking protein particle complex subunit 1
- Trafficking protein particle complex subunit 5
- Trafficking protein particle complex subunit 3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0097367 (carbohydrate derivative binding), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0032553 (ribonucleotide binding), GO:0019003 (GDP binding), GO:0032555 (purine ribonucleotide binding), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0017076 (purine nucleotide binding), GO:0016462 (pyrophosphatase activity), GO:0019001 (guanyl nucleotide binding), GO:0032561 (guanyl ribonucleotide binding), GO:0017111 (ribonucleoside triphosphate phosphatase activity), GO:0003924 (GTPase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0019222 (regulation of metabolic process), GO:0051234 (establishment of localization), GO:0050794 (regulation of cellular process), GO:0051234 (establishment of localization), GO:0140352 (export from cell), GO:0033036 (macromolecule localization), GO:0009894 (regulation of catabolic process), GO:0046907 (intracellular transport), GO:0006810 (transport), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0032940 (secretion by cell), GO:0031323 (regulation of cellular metabolic process), GO:0051668 (localization within membrane), GO:0045184 (establishment of protein localization), GO:0006887 (exocytosis), GO:0016197 (endosomal transport), GO:0098876 (vesicle-mediated transport to the plasma membrane), GO:0015031 (protein transport), GO:0016482 (cytosolic transport), GO:0071702 (organic substance transport), GO:0016192 (vesicle-mediated transport), GO:0008104 (protein localization), GO:0031329 (regulation of cellular catabolic process), GO:0090150 (establishment of protein localization to membrane), GO:0072657 (protein localization to membrane), GO:0048193 (Golgi vesicle transport), GO:0006893 (Golgi to plasma membrane transport), GO:0072659 (protein localization to plasma membrane), GO:0042147 (retrograde transport, endosome to Golgi), GO:1990778 (protein localization to cell periphery), GO:0043001 (Golgi to plasma membrane protein transport), GO:0061951 (establishment of protein localization to plasma membrane), GO:0006892 (post-Golgi vesicle-mediated transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0071944 (cell periphery), GO:0031984 (organelle subcompartment), GO:0005615 (extracellular space), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005576 (extracellular region), GO:0031090 (organelle membrane), GO:0030141 (secretory granule), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043230 (extracellular organelle), GO:0098791 (Golgi apparatus subcompartment), GO:0005794 (Golgi apparatus), GO:0070062 (extracellular exosome), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0032588 (trans-Golgi network membrane), GO:0012506 (vesicle membrane), GO:0005802 (trans-Golgi network), GO:0065010 (extracellular membrane-bounded organelle), GO:0005769 (early endosome), GO:0030667 (secretory granule membrane), GO:0010008 (endosome membrane), GO:0031982 (vesicle), GO:0030659 (cytoplasmic vesicle membrane), GO:1903561 (extracellular vesicle), GO:0097708 (intracellular vesicle), GO:0031901 (early endosome membrane)