Protein: Non-homologous end-joining factor 1 (Protein cernunnos) (XRCC4-like factor) (Q3KNJ2)
Organism: Mus musculus (Mouse)
Function: DNA repair protein involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination. Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends. Interacts with POLL (DNA polymerase lambda); promoting POLL recruitment to double-strand breaks (DSBs) and stimulation of the end-filling activity of POLL (By similarity). May act in concert with XRCC5-XRCC6 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are non-complementary or partially complementary. Associates with XRCC4 to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired (By similarity). The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other (By similarity). The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors (By similarity). Binds DNA in a length-dependent manner (By similarity).
Subcellular location: Nucleus {ECO:0000250|UniProtKB:Q9H9Q4}. Chromosome {ECO:0000250|UniProtKB:Q9H9Q4}. Note=Localizes to site of double-strand breaks; recruitment is dependent on XRCC5-XRCC6 (Ku) heterodimer. {ECO:0000250|UniProtKB:Q9H9Q4}.
Sequence: MEELEQDLLLQPWAWLQLAENSLLAKVSITKHGYALLISDLQQVWHEQVDTSVVSQRAKELNKRLTAPPAALLCHLDEALRPLFKDSAHPSKATFSCDRGEEGLILRVQSELSGLPFSWHFHCIPASSSLVSQHLIHPLMGVSLALQSHVRELAALLRMKDLEIQAYQESGAVLSRSRLKTEPFEENSFLEQFMAEKLPEACAVGDGKPFAMSLQSLYVAVTKQQIQARQAHKDSGETQASSSTSPRGTDNQPEEPVSLSSTLSEPEYEPVAASGPMHRARLVKSKRKKPRGLFS
InterPro domains: - IPR038051: XRCC4-like, N-terminal domain superfamily (homologous_superfamily) [1-139]
- IPR053829: XLF-like, coiled-coil region (domain) [128-175]
- IPR015381: XLF-like, N-terminal (domain) [12-124]
- IPR052287: Non-homologous end-joining factor (family) [1-294]
Interaction partners: - X-ray repair cross-complementing protein 6
- DNA repair protein XRCC4
- DNA-dependent protein kinase catalytic subunit
- DNA ligase 4
- X-ray repair cross-complementing protein 5
- Protein PAXX
- TP53-binding protein 1
- Protein artemis
- Serine-protein kinase ATM
- DNA repair protein XRCC1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0019899 (enzyme binding), GO:0003677 (DNA binding), GO:0070182 (DNA polymerase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0002376 (immune system process), GO:0032501 (multicellular organismal process), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0009628 (response to abiotic stimulus), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0001775 (cell activation), GO:0002440 (production of molecular mediator of immune response), GO:0045321 (leukocyte activation), GO:0002200 (somatic diversification of immune receptors), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0002520 (immune system development), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0044238 (primary metabolic process), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0046483 (heterocycle metabolic process), GO:0009314 (response to radiation), GO:0048731 (system development), GO:0002562 (somatic diversification of immune receptors via germline recombination within a single locus), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046649 (lymphocyte activation), GO:0002377 (immunoglobulin production), GO:1901360 (organic cyclic compound metabolic process), GO:0016445 (somatic diversification of immunoglobulins), GO:0006139 (nucleobase-containing compound metabolic process), GO:0033554 (cellular response to stress), GO:0044260 (cellular macromolecule metabolic process), GO:0030097 (hemopoiesis), GO:0002521 (leukocyte differentiation), GO:0006974 (cellular response to DNA damage stimulus), GO:0033151 (V(D)J recombination), GO:0030098 (lymphocyte differentiation), GO:0016447 (somatic recombination of immunoglobulin gene segments), GO:0090304 (nucleic acid metabolic process), GO:0006259 (DNA metabolic process), GO:0042110 (T cell activation), GO:0010212 (response to ionizing radiation), GO:0010467 (gene expression), GO:0042113 (B cell activation), GO:0030217 (T cell differentiation), GO:0006310 (DNA recombination), GO:0006281 (DNA repair), GO:0006266 (DNA ligation), GO:1903131 (mononuclear cell differentiation), GO:0030183 (B cell differentiation), GO:0016444 (somatic cell DNA recombination), GO:0006302 (double-strand break repair), GO:0006303 (double-strand break repair via nonhomologous end joining)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:1902494 (catalytic complex), GO:0090734 (site of DNA damage), GO:0001650 (fibrillar center), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0140535 (intracellular protein-containing complex), GO:0043233 (organelle lumen), GO:1990391 (DNA repair complex), GO:0035861 (site of double-strand break), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005694 (chromosome), GO:0005634 (nucleus)