Protein: Serine/threonine-protein kinase PLK4 (EC 2.7.11.21) (Polo-like kinase 4) (PLK-4) (Serine/threonine-protein kinase Sak) (Q6PAD2)
Organism: Xenopus laevis (African clawed frog)
Function: Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as sass6, cenpj/cpap, ccp110, cep135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles (By similarity).
Subcellular location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:O00444}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:O00444}. Note=Associates with centrioles throughout the cell cycle (By similarity). Component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells that can generate more than 100 centrioles. {ECO:0000250, ECO:0000269|PubMed:24075808}.
Sequence: MAGSIGERREDFKVLNLLGKGSFACVYRAQSINTGIDVAIKMIDKKAMQKVGMVQRVRNEVEIHCQLKHPSILELYNYFEDSNYVYLILEMCHNGEVNRYLKNRKKPFAEDEARHFMHQIVTGMLYLHSHGILHRDLTLSNLLLSSDMNIKIADFGLATQLKMPNEKHFTMCGTPNYIAPEIATRSAHGLESDVWSLGCMLYTFLVGRPPFDTDTVKNTLNKIVLADYEMPDFVSREAKDLIFQLLRKNPADRLSLSSVLDHAFMTGFSNVQSKVMGAVEDSMDSGHATISTGFTGSSGVSISGRFQEKRILSGPSLPNKVNIFQFKDKHPTERSNGGSFHNTQRENNDFSEGNGRKPVACEDRPHSRYLRRAHSSDRSGTSQSQTYAKPSSYSERCHSVEMLAKPTHLKGYRTSSPPNSYGDIPQMFTDERSLERHTSPPVKEKTPSEFMGPAKQTAPRSNDKAETVQQWFGAMQLNGQFKNTPDTSSVSNMGGDFYSQQATQNGAPQYAWNDVKRKKNTDSSIESVLLGIKKNPGTGQRKAEKSQFGEQSKSRVPQQAFGSSTLRSIISPLNAERLKPIRQKTKNAVVSILESGEVCMEFLKEQNSQERVKEVLRISCDGNLIYVYHPNEGKGFPLVDRPPSPPENRLSYTFDSLPEKYWKKYQYAAKFIKLVRSKTPKVTYYTRYAKCMLMENSPTADVEVCFYDGAKIHKTSDVIRVIEKSGRSYTLEGSRLSTLSDEVRSYLDHANESHCVCLSLESAINTEEKKGENISLFPITFGRRPALAESPKTQPTPSVDSARERKEEQSYVNRVLHGSAASPPQMPNLNPSLISYDGSVFSATTVQPSPTSNIHNTPDHAQVLKSVFVENVGWASQLNSGAVWVQFNDGSQLVVQPGVSSIIYTAPNGQITRHGENDKLPEYIKSKLQCLSSILMLFASSSSR
InterPro domains: - IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [9-266]
- IPR033696: Plk4, C-terminal polo-box domain (domain) [860-939]
- IPR017441: Protein kinase, ATP binding site (binding_site) [18-41]
- IPR033699: Plk4, first cryptic polo-box domain (domain) [565-678]
- IPR000719: Protein kinase domain (domain) [12-265]
- IPR046437: Serine/threonine-protein kinase, first cryptic polo-box domain superfamily (homologous_superfamily) [561-679]
- IPR008266: Tyrosine-protein kinase, active site (active_site) [132-144]
- IPR000959: POLO box domain (domain) [862-940]
- IPR047108: Plk4-like, second cryptic polo-box domain superfamily (homologous_superfamily) [680-786]
- IPR033698: Plk4, second cryptic polo-box domain (domain) [678-791]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0004672 (protein kinase activity), GO:0042802 (identical protein binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0007049 (cell cycle), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0007017 (microtubule-based process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0007099 (centriole replication), GO:0051726 (regulation of cell cycle), GO:0090068 (positive regulation of cell cycle process), GO:1901564 (organonitrogen compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0019538 (protein metabolic process), GO:0016043 (cellular component organization), GO:0051298 (centrosome duplication), GO:0051130 (positive regulation of cellular component organization), GO:0000226 (microtubule cytoskeleton organization), GO:0031023 (microtubule organizing center organization), GO:0032886 (regulation of microtubule-based process), GO:0051128 (regulation of cellular component organization), GO:0007098 (centrosome cycle), GO:0010564 (regulation of cell cycle process), GO:0043170 (macromolecule metabolic process), GO:0044089 (positive regulation of cellular component biogenesis), GO:0044087 (regulation of cellular component biogenesis), GO:0045787 (positive regulation of cell cycle), GO:0006793 (phosphorus metabolic process), GO:0036211 (protein modification process), GO:0098534 (centriole assembly), GO:0046599 (regulation of centriole replication), GO:0043412 (macromolecule modification), GO:0030030 (cell projection organization), GO:1902117 (positive regulation of organelle assembly), GO:0033043 (regulation of organelle organization), GO:0006796 (phosphate-containing compound metabolic process), GO:0046605 (regulation of centrosome cycle), GO:0006996 (organelle organization), GO:0010638 (positive regulation of organelle organization), GO:1902115 (regulation of organelle assembly), GO:0010824 (regulation of centrosome duplication), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0007010 (cytoskeleton organization), GO:0051493 (regulation of cytoskeleton organization), GO:0030031 (cell projection assembly), GO:0006468 (protein phosphorylation), GO:0120036 (plasma membrane bounded cell projection organization), GO:0044782 (cilium organization), GO:0051495 (positive regulation of cytoskeleton organization), GO:0016310 (phosphorylation), GO:0120031 (plasma membrane bounded cell projection assembly), GO:0140694 (non-membrane-bounded organelle assembly), GO:0060271 (cilium assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)