Protein: Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 2) (Ketol-acid reductoisomerase type II) (P05989)
Organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Function: Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.
Sequence: MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVGCGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLVVNLTPDKQHSDVVRSVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTEVREEYKRGFGVPTLIAVHPENDPQGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDLMGEQTILCGMLQAGSLLCFDKLVAEGTDPAYAEKLIQFGWETITEALKQGGITLMMDRLSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREETGKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLHELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGSAIPEGAVDNAQLRDVNDAIRSHAIEQVGKKLRGYMTDMKRIAVAG
InterPro domains: - IPR008927: 6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily (homologous_superfamily) [210-484]
- IPR036291: NAD(P)-binding domain superfamily (homologous_superfamily) [30-209]
- IPR013023: Ketol-acid reductoisomerase (family) [1-485]
- IPR000506: Ketol-acid reductoisomerase, C-terminal (domain) [209-484]
- IPR013328: 6-phosphogluconate dehydrogenase, domain 2 (homologous_superfamily) [209-491]
- IPR013116: Ketol-acid reductoisomerase, N-terminal (domain) [15-208]
Interaction partners: - Dihydroxy-acid dehydratase
- Dihydroxyacid dehydratase
- Acetolactate synthase
- 3-isopropylmalate dehydrogenase
- Acetolactate synthase isozyme 3 small subunit
- Acetolactate synthase isozyme 3 large subunit
- 2-isopropylmalate synthase
- Acetolactate synthase isozyme 1 small subunit
- Acetolactate synthase
- Acetolactate synthase isozyme 2 small subunit
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:1901265 (nucleoside phosphate binding), GO:0046983 (protein dimerization activity), GO:0043168 (anion binding), GO:0042802 (identical protein binding), GO:0000166 (nucleotide binding), GO:0043169 (cation binding), GO:0042803 (protein homodimerization activity), GO:0070402 (NADPH binding), GO:0046872 (metal ion binding), GO:0017076 (purine nucleotide binding), GO:0000287 (magnesium ion binding), GO:0030554 (adenyl nucleotide binding), GO:0050661 (NADP binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006575 (cellular modified amino acid metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0006766 (vitamin metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0042398 (cellular modified amino acid biosynthetic process), GO:0006767 (water-soluble vitamin metabolic process), GO:0009110 (vitamin biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0016053 (organic acid biosynthetic process), GO:0008652 (amino acid biosynthetic process), GO:0015939 (pantothenate metabolic process), GO:0043604 (amide biosynthetic process), GO:0009081 (branched-chain amino acid metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0015940 (pantothenate biosynthetic process), GO:0042364 (water-soluble vitamin biosynthetic process), GO:0006549 (isoleucine metabolic process), GO:0009082 (branched-chain amino acid biosynthetic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0006573 (valine metabolic process), GO:0072330 (monocarboxylic acid biosynthetic process), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)