Protein: Dihydropyrimidine dehydrogenase [NADP(+)] (DHPDHase) (DPD) (EC 1.3.1.2) (Dihydrothymine dehydrogenase) (Dihydrouracil dehydrogenase) (Q28007)
Organism: Bos taurus (Bovine)
Function: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil.
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:Q12882}.
Sequence: MAPVLSKDVADIESILALNPRTQSRATLRSTLAKKLDKKHWKRNPDKNCFNCEKLENNFDDIKHTTLGERGALREAMRCLKCADAPCQKSCPTNLDIKSFITSISNKNYYGAAKMIFSDNPLGLTCGMVCPTSDLCVGGCNLYATEEGPINIGGLQQYATEVFKAMNIPQIRNPSLPPPEKMPEAYSAKIALLGAGPASISCASFLARLGYNDITIFEKQEYVGGISTSEIPQFRLPYDVVNFEIELMKDLGVKIICGKSLSVNDITLSTLKEEGYKAAFIGIGLPEPKKDHIFQGLTQDQGFYTSKDFLPLVAKSSKAGMCACHSPLLSIRGTVIVLGAGDTAFDCATSALRCGARRVFIVFRKGFVNIRAVPEEVELAREEKCEFLPFLSPRKVIVKGGRIVAMQFVRTEQDETGKWNEDGDQIACLKADVVISAFGSVLSDPKVKEALSPIKFNRWDLPEVDPETMQTSEPWVFAGGDVVGIANTTVEAVNDGKQASWYIHRYIQSQYGASVSAKPELPLFYTPIDLVDISVEMAALKFTNPFGLASATPTTSSSMIRRAFEAGWAFALTKTFSLDKDIVTNVSPRIIRGTTSGPMYGPGQSSFLNIELISEKTAAYWCQSVTELKADFPDNIVIASIMCSYNRNDWMELSRKAEASGADALELNLSCPHGMGERGMGLACGQDPELVRNICRWVRQAVRIPFFAKLTPNVTDIVSIARAAKEGGANGVTATNTVSGLMGLKADGTPWPAVGREKRTTYGGVSGTAIRPIALRAVTTIARALPEFPILATGGIDSAESGLQFLHGGASVLQVCSAIQNQDFTIIQDYCTGLKALLYLKSIEELQDWDGQSPATKSHQKGKPVPCIAELVGKKLPSFGPYLEKCKKIIAEEKLRLKKENVTVLPLERNHFIPKKPIPSVKDVIGKALQYLGTYGELNNTEQVVAVIDEEMCINCGKCYMTCNDSGYQAIQFDPETHLPTVTDTCTGCTLCLSVCPIIDCIKMVSRTTPYEPKRGLPLAVNPVS
InterPro domains: - IPR017896: 4Fe-4S ferredoxin-type, iron-sulphur binding domain (domain) [944-1007]
- IPR009051: Alpha-helical ferredoxin (homologous_superfamily) [27-183]
- IPR028261: Dihydroprymidine dehydrogenase domain II (domain) [57-167]
- IPR023753: FAD/NAD(P)-binding domain (domain) [189-496]
- IPR005720: Dihydroorotate dehydrogenase, catalytic (domain) [534-838]
- IPR013785: Aldolase-type TIM barrel (homologous_superfamily) [533-844]
- IPR017900: 4Fe-4S ferredoxin, iron-sulphur binding, conserved site (conserved_site) [986-997]
- IPR036188: FAD/NAD(P)-binding domain superfamily (homologous_superfamily) [184-532]
Interaction partners: - Uridine 5'-monophosphate synthase
- Dihydropyrimidinase
- Uridine phosphorylase
- Uridine phosphorylase
- Beta-ureidopropionase 1
- Uracil phosphoribosyltransferase homolog
- Purine nucleoside phosphorylase
- Cytochrome b5 reductase 4
- NADH-cytochrome b5 reductase
- Oxidoreductase NAD-binding domain-containing protein 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016627 (oxidoreductase activity, acting on the CH-CH group of donors), GO:0016628 (oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0009112 (nucleobase metabolic process), GO:0072527 (pyrimidine-containing compound metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0046112 (nucleobase biosynthetic process), GO:0006206 (pyrimidine nucleobase metabolic process), GO:0072528 (pyrimidine-containing compound biosynthetic process), GO:0019856 (pyrimidine nucleobase biosynthetic process), GO:0006207 ('de novo' pyrimidine nucleobase biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)