Protein: Xaa-Pro aminopeptidase 3 (X-Pro aminopeptidase 3) (EC 3.4.11.9) (Aminopeptidase P3) (APP3) (B7ZMP1)
Organism: Mus musculus (Mouse)
Function: Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Leu-Pro-Ala. Also shows low activity towards peptides with Ala or Ser at the P1 position. Promotes TNFRSF1B-mediated phosphorylation of MAPK8/JNK1 and MAPK9/JNK2, suggesting a function as an adapter protein for TNFRSF1B; the effect is independent of XPNPEP3 peptidase activity. May inhibit apoptotic cell death induced via TNF-TNFRSF1B signaling.
Subcellular location: Mitochondrion {ECO:0000250|UniProtKB:Q9NQH7}. Cytoplasm {ECO:0000250|UniProtKB:Q9NQH7}. Note=Mainly mitochondrial. Translocates to the cytoplasm following TNFRSF1B activation. {ECO:0000250|UniProtKB:Q9NQH7}.
Sequence: MPSLLSTPKLAPVLARLRGLSGCMSCLQRRYSLQPAPVKKIPNRYLGQPSPVTHPHLLRPGEVTPGLSQVEYALRRHKLMALVHKEAQGHSGTDHTVVVLSNPTYYMSNDIPYTFHQDNNFLYLCGFQEPDSILVLQSFSGKQLPSHKAMLFVPRRDPGRELWDGPRSGTDGAIALTGVDEAYPLEEFQHLLPKLRAETNMVWYDWMKPSHAQLHSDYMQPLTEAKARSKNKVRSVQQLIQRLRLVKSPSEIKRMQIAGKLTSEAFIETMFASKAPIDEAFLYAKFEFECRARGADILAYPPVVAGGNRSNTLHYVKNNQLIKDGEMVLLDGGCESSCYVSDITRTWPVNGRFTAPQAELYEAVLEIQRACLTLCSPGTSLENIYSMMLTLIGQKLKDLGITKTSKESAFKAARKYCPHHVGHYLGMDVHDTPDMPRSLPLQPGMVITVEPGIYIPEDDRDAPEKFRGLGVRIEDDVVVTQDSPLILSADCPKEMNDIEQICSRTS
InterPro domains: - IPR036005: Creatinase/aminopeptidase-like (homologous_superfamily) [247-506]
- IPR052433: Xaa-Pro dipeptidase-like (family) [44-503]
- IPR029149: Creatinase/Aminopeptidase P/Spt16, N-terminal (homologous_superfamily) [66-247]
- IPR000994: Peptidase M24 (domain) [254-481]
- IPR007865: Aminopeptidase P, N-terminal (domain) [67-213]
Interaction partners: - Mitochondrial-processing peptidase subunit alpha
- Mitochondrial intermediate peptidase
- Mitochondrial-processing peptidase subunit beta
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0008233 (peptidase activity), GO:0008238 (exopeptidase activity), GO:0004177 (aminopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0032501 (multicellular organismal process), GO:0065008 (regulation of biological quality), GO:0071704 (organic substance metabolic process), GO:0003008 (system process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0003014 (renal system process), GO:0008217 (regulation of blood pressure), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0043170 (macromolecule metabolic process), GO:0003013 (circulatory system process), GO:0050886 (endocrine process), GO:0006508 (proteolysis), GO:0008015 (blood circulation), GO:0001990 (regulation of systemic arterial blood pressure by hormone), GO:0003073 (regulation of systemic arterial blood pressure), GO:0003081 (regulation of systemic arterial blood pressure by renin-angiotensin), GO:0003044 (regulation of systemic arterial blood pressure mediated by a chemical signal), GO:0001991 (regulation of systemic arterial blood pressure by circulatory renin-angiotensin)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)