Protein: Small ubiquitin-related modifier 1 (SUMO-1) (A7WLH8)
Organism: Sus scrofa (Pig)
Function: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3.
Subcellular location: Nucleus membrane {ECO:0000250|UniProtKB:P63165}. Nucleus speckle {ECO:0000250|UniProtKB:P63166}. Cytoplasm {ECO:0000250|UniProtKB:P63165}. Nucleus, PML body {ECO:0000250|UniProtKB:P63165}. Cell membrane {ECO:0000250|UniProtKB:P63165}. Nucleus {ECO:0000250|UniProtKB:P63165}. Note=Recruited by BCL11A into the nuclear body (By similarity). In the presence of ZFHX3, sequesterd to nuclear body (NB)-like dots in the nucleus some of which overlap or closely associate with PML body (By similarity). {ECO:0000250|UniProtKB:P63165, ECO:0000250|UniProtKB:P63166}.
Sequence: MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTV
InterPro domains: - IPR046332: Small ubiquitin-related modifier 1, Ubl domain (domain) [21-96]
- IPR022617: Rad60/SUMO-like domain (domain) [22-91]
- IPR000626: Ubiquitin-like domain (domain) [20-97]
- IPR029071: Ubiquitin-like domain superfamily (homologous_superfamily) [2-98]
Interaction partners: - E3 SUMO-protein ligase RanBP2
- SUMO-conjugating enzyme UBC9
- Protein inhibitor of activated STAT 1.
- Protein inhibitor of activated STAT 4.
- Cellular tumor antigen p53
- SUMO specific peptidase 1.
- protein kinase C
- Small ubiquitin-related modifier
- Small ubiquitin-related modifier 2
- SUMO specific peptidase 5
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0043170 (macromolecule metabolic process), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0006996 (organelle organization), GO:0070647 (protein modification by small protein conjugation or removal), GO:0006997 (nucleus organization), GO:0018193 (peptidyl-amino acid modification), GO:0018205 (peptidyl-lysine modification), GO:0032446 (protein modification by small protein conjugation), GO:0030575 (nuclear body organization), GO:0016925 (protein sumoylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)