Protein: Iron(III) enterobactin esterase (EC 3.1.1.108) (Enterochelin esterase) (Ferric enterobactin esterase) (P13039)
Organism: Escherichia coli (strain K12)
Function: Catalyzes the hydrolysis of ferric enterobactin (Fe-Ent). Is responsible for the release of iron from ferric enterobactin. Also catalyzes the hydrolysis of iron-free enterobactin (Ent). Cleavage of ferric enterobactin results in a mixture of three hydrolysis products, 2,3-dihydroxybenzoylserine (DHBS), the linear dimer (DHBS)2 and the linear trimer (DHBS)3, while cleavage of iron-free enterobactin yields only the monomer. Hydrolysis of ferric enterobactin is less efficient than hydrolysis of unliganded enterobactin. It also cleaves the aluminum (III) complex at a rate similar to the ferric complex.
Subcellular location: Cytoplasm {ECO:0000305|PubMed:1534808}.
Sequence: MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYSTIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIFSVPSPDRLELREGWRKLLPQAIADPLNLQSWKGGRGHAVSALEMPQAPLQPGWDCPQAPEIPAKEIIWKSERLKKSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSLTHRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTVVAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEGLRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLWQPLFHDRS
InterPro domains: - IPR021764: Enterochelin esterase, N-terminal (domain) [29-155]
- IPR013783: Immunoglobulin-like fold (homologous_superfamily) [1-144]
- IPR029058: Alpha/Beta hydrolase fold (homologous_superfamily) [145-400]
- IPR000801: Esterase-like (family) [173-390]
- IPR014756: Immunoglobulin E-set (homologous_superfamily) [5-150]
- IPR050583: Mycobacterial A85 antigen (family) [72-395]
Interaction partners: - Enterobactin synthase component F
- Ferric enterobactin-binding periplasmic protein FepB
- Isochorismate synthase EntC
- Enterobactin biosynthesis protein YbdZ
- Ferrienterobactin receptor
- Enterobactin exporter EntS
- Enterobactin synthase component E
- Enterobactin synthase component B
- 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
- Ferric enterobactin transport system permease protein FepG
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0008233 (peptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0009237 (siderophore metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044282 (small molecule catabolic process), GO:0046483 (heterocycle metabolic process), GO:0044248 (cellular catabolic process), GO:0006082 (organic acid metabolic process), GO:0006066 (alcohol metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0019757 (glycosinolate metabolic process), GO:0019760 (glucosinolate metabolic process), GO:0016054 (organic acid catabolic process), GO:0044273 (sulfur compound catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:1901136 (carbohydrate derivative catabolic process), GO:0043436 (oxoacid metabolic process), GO:0019439 (aromatic compound catabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0009116 (nucleoside metabolic process), GO:0046700 (heterocycle catabolic process), GO:1901657 (glycosyl compound metabolic process), GO:0034656 (nucleobase-containing small molecule catabolic process), GO:0072521 (purine-containing compound metabolic process), GO:0018958 (phenol-containing compound metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:1901658 (glycosyl compound catabolic process), GO:0042436 (indole-containing compound catabolic process), GO:1901606 (alpha-amino acid catabolic process), GO:0046395 (carboxylic acid catabolic process), GO:0042278 (purine nucleoside metabolic process), GO:0009119 (ribonucleoside metabolic process), GO:0046128 (purine ribonucleoside metabolic process), GO:0032787 (monocarboxylic acid metabolic process), GO:0033869 (nucleoside bisphosphate catabolic process), GO:0072329 (monocarboxylic acid catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex)