Protein: E3 ubiquitin-protein ligase CSU1 (EC 2.3.2.27) (Protein COP1 SUPPRESSOR 1) (RING-type E3 ubiquitin transferase CSU1) (Q9SY88)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: RING-finger E3 ubiquitin-protein ligase that plays an major role in maintaining COP1 homeostasis in darkness. Negatively regulates COP1 protein accumulation by targeting COP1 for ubiquitination and subsequent proteasomal degradation in dark-grown seedlings. Negatively regulates the accumulation of SPA1 protein in the dark.
Subcellular location: Nucleus {ECO:0000269|PubMed:24838976}. Nucleus speckle {ECO:0000269|PubMed:24838976}. Note=Is recruited to nuclear speckles by COP1 in the dark. {ECO:0000269|PubMed:24838976}.
Sequence: MPQRHSKNNNDLAYFTYDEKKKLGYGTQRERLGRDSIKPFDACSLCLKPFIDPMCCHKGHVFCRECILECFLAQKKDIQRRLAAHSSQKKQDKDEEEERLMLQKARELDEFDQQNHSAMPRNSDKNHNEDKNGFHGANSVKTTSFEEEALRTMKAFWLPSATPAASVRVDAPETHTVCPEGKEKLKLKNLFAIRFTEDNSEEEETKTKSASSSSYDKSYICPSCKVTLTNTMSLVALSSCGHVFCKKCAEKFMPVDKVCLVCDKPCKDRNLVGLKKGGTGFAEHDDHLEAKEYKHLGSGSGLGLVRPVKT
InterPro domains: - IPR017907: Zinc finger, RING-type, conserved site (conserved_site) [240-249]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [16-281]
- IPR031790: Nitric oxide synthase-interacting protein, zinc-finger (domain) [5-75]
- IPR016818: Nitric oxide synthase-interacting protein (family) [2-307]
- IPR001841: Zinc finger, RING-type (domain) [43-263]
Interaction partners: - Protein COP1 SUPPRESSOR 2
- Probable E3 ubiquitin-protein ligase RHA1A
- At3g29270
- E3 ubiquitin-protein ligase RMA
- NADH dehydrogenase 1 beta subcomplex subunit 9
- Probable E3 ubiquitin-protein ligase WAVH2
- SBP family protein
- AP-3 complex subunit mu
- Putative E3 ubiquitin-protein ligase ORTHRUS 4
- B-box zinc finger protein 22
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0019787 (ubiquitin-like protein transferase activity), GO:0004842 (ubiquitin-protein transferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0008380 (RNA splicing), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0000398 (mRNA splicing, via spliceosome), GO:0000375 (RNA splicing, via transesterification reactions), GO:0000380 (alternative mRNA splicing, via spliceosome), GO:0000377 (RNA splicing, via transesterification reactions with bulged adenosine as nucleophile)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)