Protein: 4-aminobutyrate aminotransferase GabT (EC 2.6.1.19) (5-aminovalerate transaminase) (EC 2.6.1.48) (GABA aminotransferase) (GABA-AT) (Gamma-amino-N-butyrate transaminase) (GABA transaminase) (Glutamate:succinic semialdehyde transaminase) (L-AIBAT) (P22256)
Organism: Escherichia coli (strain K12)
Function: Pyridoxal phosphate-dependent enzyme that catalyzes transamination between primary amines and alpha-keto acids. Catalyzes the transfer of the amino group from gamma-aminobutyrate (GABA) to alpha-ketoglutarate (KG) to yield succinic semialdehyde (SSA) and glutamate. Thereby functions in a GABA degradation pathway that allows some E.coli strains to utilize GABA as a nitrogen source for growth. Also catalyzes the conversion of 5-aminovalerate to glutarate semialdehyde, as part of a L-lysine degradation pathway that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate.
Sequence: MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASSPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDGLLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGPYYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ
InterPro domains: - IPR005814: Aminotransferase class-III (family) [7-424]
- IPR050103: Class-III Pyridoxal-phosphate-dependent Aminotransferase (family) [3-424]
- IPR004632: 4-aminobutyrate aminotransferase, bacterial (family) [9-420]
- IPR015421: Pyridoxal phosphate-dependent transferase, major domain (homologous_superfamily) [59-318]
- IPR049704: Aminotransferases class-III pyridoxal-phosphate attachment site (conserved_site) [236-273]
- IPR015424: Pyridoxal phosphate-dependent transferase (homologous_superfamily) [20-423]
- IPR015422: Pyridoxal phosphate-dependent transferase, small domain (homologous_superfamily) [24-418]
Interaction partners: - Succinate-semialdehyde dehydrogenase [NADP
- Succinate semialdehyde dehydrogenase [NAD
- Gamma-aminobutyraldehyde dehydrogenase
- L-2-hydroxyglutarate dehydrogenase
- Glutamate decarboxylase beta
- Gamma-aminobutyric acid permease
- Glutamate decarboxylase alpha
- Glutarate 2-hydroxylase
- Fatty acid oxidation complex subunit alpha
- Fatty acid oxidation complex subunit alpha
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0016740 (transferase activity), GO:0043168 (anion binding), GO:0019842 (vitamin binding), GO:0016769 (transferase activity, transferring nitrogenous groups), GO:0070279 (vitamin B6 binding), GO:0008483 (transaminase activity), GO:0030170 (pyridoxal phosphate binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0006807 (nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0009308 (amine metabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0006576 (biogenic amine metabolic process), GO:0009310 (amine catabolic process), GO:0042402 (cellular biogenic amine catabolic process), GO:0006595 (polyamine metabolic process), GO:0009445 (putrescine metabolic process), GO:0006598 (polyamine catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)