Protein: Methyl-CpG-binding domain protein 4 (EC 3.2.2.-) (Methyl-CpG-binding protein MBD4) (Mismatch-specific DNA N-glycosylase) (Q9Z2D7)
Organism: Mus musculus (Mouse)
Function: Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
Subcellular location: Nucleus {ECO:0000269|PubMed:9774669}. Note=Nuclear, in discrete foci.
Sequence: MESPNLGDNRVRGESLVPDPPWDRCKEDIAVGLGGVGEDGKDLVISSERSSLLQEPTASTLSSTTATEGHKPVPCGWERVVKQRLSGKTAGKFDVYFISPQGLKFRSKRSLANYLLKNGETFLKPEDFNFTVLPKGSINPGYKHQSLAALTSLQPNETDVSKQNLKTRSKWKTDVLPLPSGTSESPESSGLSNSNSACLLLREHRDIQDVDSEKRRKSKRKVTVLKGTASQKTKQKCRKSLLESTQRNRKRASVVQKVGADRELVPQESQLNRTLCPADACARETVGLAGEEKSPSPGLDLCFIQVTSGTTNKFHSTEAAGEANREQTFLESEEIRSKGDRKGEAHLHTGVLQDGSEMPSCSQAKKHFTSETFQEDSIPRTQVEKRKTSLYFSSKYNKEALSPPRRKSFKKWTPPRSPFNLVQEILFHDPWKLLIATIFLNRTSGKMAIPVLWEFLEKYPSAEVARAADWRDVSELLKPLGLYDLRAKTIIKFSDEYLTKQWRYPIELHGIGKYGNDSYRIFCVNEWKQVHPEDHKLNKYHDWLWENHEKLSLS
InterPro domains: - IPR011257: DNA glycosylase (homologous_superfamily) [413-550]
- IPR016177: DNA-binding domain superfamily (homologous_superfamily) [51-166]
- IPR045138: Methyl-CpG binding protein MeCP2/MBD4 (family) [6-552]
- IPR017352: Methyl-CpG-binding domain protein 4 (family) [1-554]
- IPR001739: Methyl-CpG DNA binding (domain) [63-140]
Interaction partners: - G/T mismatch-specific thymine DNA glycosylase
- DNA
- Single-strand selective monofunctional uracil DNA glycosylase
- DNA
- DNA
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0016798 (hydrolase activity, acting on glycosyl bonds), GO:0140097 (catalytic activity, acting on DNA), GO:0016799 (hydrolase activity, hydrolyzing N-glycosyl compounds), GO:0003677 (DNA binding), GO:0019104 (DNA N-glycosylase activity), GO:0003690 (double-stranded DNA binding), GO:0043565 (sequence-specific DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0006259 (DNA metabolic process), GO:0006281 (DNA repair), GO:0006284 (base-excision repair)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)