Protein: 9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic (LeNCED1) (SlNCED1) (EC 1.13.11.51) (Nine-cis-epoxycarotenoid dioxygenase 1) (O24023)
Organism: Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Function: Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid (ABA) biosynthesis from carotenoids. Required for ABA accumulation upon drought (Ref.2). Required for ABA-mediated regulation of anther/pollen development, including metabolism, cell wall modification and transcription level. Positive regulator of fruit ripening involved in the biosynthesis of abscisic acid (ABA); initiates ABA biosynthesis at the onset of fruit ripening. Modulates the degree of pigmentation and carotenoid composition as well as pectin catabolism during ripening and may regulate the ethylene production and action in climacteric tomato fruit.
Subcellular location: Plastid, chloroplast stroma {ECO:0000250|UniProtKB:Q9LRR7}. Note=Partially bound to the thylakoid. {ECO:0000250|UniProtKB:Q9LRR7}.
Sequence: MATTTSHATNTWIKTKLSMPSSKEFGFASNSISLLKNQHNRQSLNINSSLQAPPILHFPKQSSNYQTPKNNTISHPKQENNNSSSSSTSKWNLVQKAAAMALDAVESALTKHELEHPLPKTADPRVQISGNFAPVPENPVCQSLPVTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYACRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSKGTGVANAGLVYFNNRLLAMSEDDLPYHVKVTPTGDLKTEGRFDFDGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITENFVVIPDQQVVFKMSEMIRGGSPVVYDKNKVSRFGILDKYAKDGSDLKWVEVPDCFCFHLWNAWEEAETDEIVVIGSCMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRKSIIENPDEQVNLEAGMVNRNKLGRKTEYAYLAIAEPWPKVSGFAKVNLFTGEVEKFIYGDNKYGGEPLFLPRDPNSKEEDDGYILAFVHDEKEWKSELQIVNAMSLKLEATVKLPSRVPYGFHGTFINANDLANQA
InterPro domains: - IPR004294: Carotenoid oxygenase (family) [133-597]
Interaction partners: - Neoxanthin synthase, chloroplastic
- Xanthoxin dehydrogenase
- Uncharacterized protein
- Zeaxanthin epoxidase, chloroplastic
- indole-3-acetaldehyde oxidase
- indole-3-acetaldehyde oxidase
- Abscisic acid 8'-hydroxylase CYP707A2
- indole-3-acetaldehyde oxidase
- Lycopene beta cyclase, chloroplastic
- Abscisic acid 8'-hydroxylase CYP707A1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0043167 (ion binding), GO:0016491 (oxidoreductase activity), GO:0016701 (oxidoreductase activity, acting on single donors with incorporation of molecular oxygen), GO:0043169 (cation binding), GO:0051213 (dioxygenase activity), GO:0016702 (oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen), GO:0046872 (metal ion binding), GO:0046914 (transition metal ion binding), GO:0005506 (iron ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0009628 (response to abiotic stimulus), GO:0006950 (response to stress), GO:0042221 (response to chemical), GO:0009719 (response to endogenous stimulus), GO:0001101 (response to acid chemical), GO:1901700 (response to oxygen-containing compound), GO:0009414 (response to water deprivation), GO:0010035 (response to inorganic substance), GO:0009415 (response to water), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:1902074 (response to salt), GO:0097305 (response to alcohol), GO:0009737 (response to abscisic acid), GO:0033993 (response to lipid)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle)