Protein: Neuroendocrine convertase 2 (NEC 2) (EC 3.4.21.94) (DMH #5) (Prohormone convertase 2) (dPC2) (Protein amontillado) (Q9VBC7)
Organism: Drosophila melanogaster (Fruit fly)
Function: Serine endopeptidase which is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Required during embryonic and larval development, probably by proteolytically processing peptide hormones involved in hatching, larval growth and larval molting. Required for the processing and activation of Akh which maintains normal hemolymph sugar levels. Has been shown in one study to be required for processing of sli into slit N-product and slit C-product in the embryo which is necessary for lateral transverse muscle elongation but has been shown in another study not to be required for sli cleavage. Required for larval hatching. Also required for normal larval wandering behavior which occurs prior to pupariation. Required during pupal development for head eversion, leg and wing disk extension, and abdominal differentiation. Required during eye development for R8 photoreceptor cell specification by regulating processing of ligands required for the BMP and activin signaling pathways.
Subcellular location: Secreted {ECO:0000269|PubMed:10749852}.
Sequence: MAAATWSWLLAPFLLLHWASAGAGGGAGGSGAGLSGPAVFTSSFLVRFRRGVDNSFAHDVADKYGFDNLGPLVGADGHEYHFKHRTLPHARSRRSLTHTRALKSHPAVHTAVQQPGFKRVKRGLRPAVPAIHGMKFDLKVGEGNRIDEEPTDPYFPMQWYLKNTGQNGGKVRLDLNVQAAWAQGITGKNVTTAIMDDGVDYMHPDLKFNYNAEASYDFSSNDPFPYPRYTDDWFNSHGTRCAGEVAAARDNGICGVGVAYDSKIAGIRMLDQPYMTDLIEANSMGHEPHKIHIYSASWGPTDDGKTVDGPRNATMRAIVQGVNEGRNGLGNIYVWASGDGGEEDDCNCDGYAASMWTISINSAINDGQNAHYDESCSSTLASTFSNGAKDPNTGVATTDLYGKCTTTHSGTSAAAPEAAGVFALALEANPQLTWRDIQHLTVLTSKRNSLFDAKNRFHWTMNGVGLEFNHLFGFGVLDAGAMVTLSKQWHSVPPRYHCEAGELTQPQAIVMGRSLFWEIKTDACKGTDTEVNYLEHVQAVISANASRRGDLELFLTSPMGTKSMILSRRANDDDHRDGFTKWPFMTTHSWGEYPQGTWKLEARFNSPQTRHGNLLEWSLVLHGTKEAPYRTLHPSSPHSKLAIVKKAHEDKKMK
InterPro domains: - IPR038466: Peptidase S8, pro-domain superfamily (homologous_superfamily) [35-122]
- IPR002884: P domain (domain) [491-627]
- IPR015500: Peptidase S8, subtilisin-related (family) [187-425]
- IPR023827: Peptidase S8, subtilisin, Asp-active site (active_site) [192-203]
- IPR022398: Peptidase S8, subtilisin, His-active site (active_site) [237-247]
- IPR023828: Peptidase S8, subtilisin, Ser-active site (active_site) [410-420]
- IPR000209: Peptidase S8/S53 domain (domain) [187-475]
- IPR036852: Peptidase S8/S53 domain superfamily (homologous_superfamily) [149-489]
- IPR032815: Peptidase S8, pro-domain (domain) [43-122]
- IPR034182: Kexin/furin catalytic domain (domain) [150-446]
- IPR008979: Galactose-binding-like domain superfamily (homologous_superfamily) [483-629]
Interaction partners: - Neuroendocrine protein 7B2
- IA-2 protein tyrosine phosphatase, isoform C
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0017171 (serine hydrolase activity), GO:0008233 (peptidase activity), GO:0008236 (serine-type peptidase activity), GO:0004175 (endopeptidase activity), GO:0004252 (serine-type endopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0043170 (macromolecule metabolic process), GO:0051604 (protein maturation), GO:0006508 (proteolysis), GO:0010467 (gene expression), GO:0016485 (protein processing), GO:0031638 (zymogen activation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005576 (extracellular region), GO:0005615 (extracellular space)