Protein: 2-oxoglutarate dehydrogenase E1/E2 component (ODH E1/E2 component) [Includes: 2-oxoglutarate dehydrogenase E1 component (ODH E1 component) (EC 1.2.4.2) (Alpha-ketoglutarate dehydrogenase E1 component) (KDH E1 component); Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61) (2-oxoglutarate dehydrogenase complex E2 component) (ODH E2 component) (OGDC-E2) (Dihydrolipoamide succinyltransferase)] (Q8NRC3)
Organism: Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534)
Function: Catalyzes the E1 and E2 reactions as part of 2-oxoglutarate dehydrogenase (ODH) activity, to convert 2-oxoglutarate to succinyl-CoA and CO(2). OdhA has reductase activity with 2-oxoglutarate but does not react with pyruvate, and also displays transsuccinylase but no transacetylase activity. Since OdhA is not lipoylated, the succinyltransferase activity of its E2 domain is dependent on lipoyl residues of the acetyltransferase AceF.
Sequence: MSSASTFGQNAWLVDEMFQQFQKDPKSVDKEWRELFEAQGGPNTTPATTEAQPSAPKESAKPAPKAAPAAKAAPRVETKPADKTAPKAKESSVPQQPKLPEPGQTPIRGIFKSIAKNMDISLEIPTATSVRDMPARLMFENRAMVNDQLKRTRGGKISFTHIIGYAMVKAVMAHPDMNNSYDVIDGKPTLIVPEHINLGLAIDLPQKDGSRALVVAAIKETEKMNFSEFLAAYEDIVARSRKGKLTMDDYQGVTVSLTNPGGIGTRHSVPRLTKGQGTIIGVGSMDYPAEFQGASEDRLAELGVGKLVTITSTYDHRVIQGAVSGEFLRTMSRLLTDDSFWDEIFDAMNVPYTPMRWAQDVPNTGVDKNTRVMQLIEAYRSRGHLIADTNPLSWVQPGMPVPDHRDLDIETHNLTIWDLDRTFNVGGFGGKETMTLREVLSRLRAAYTLKVGSEYTHILDRDERTWLQDRLEAGMPKPTQAEQKYILQKLNAAEAFENFLQTKYVGQKRFSLEGAEALIPLMDSAIDTAAGQGLDEVVIGMPHRGRLNVLFNIVGKPLASIFNEFEGQMEQGQIGGSGDVKYHLGSEGQHLQMFGDGEIKVSLTANPSHLEAVNPVMEGIVRAKQDYLDKGVDGKTVVPLLLHGDAAFAGLGIVPETINLAKLRGYDVGGTIHIVVNNQIGFTTTPDSSRSMHYATDYAKAFGCPVFHVNGDDPEAVVWVGQLATEYRRRFGKDVFIDLVCYRLRGHNEADDPSMTQPKMYELITGRETVRAQYTEDLLGRGDLSNEDAEAVVRDFHDQMESVFNEVKEGGKKQAEAQTGITGSQKLPHGLETNISREELLELGQAFANTPEGFNYHPRVAPVAKKRVSSVTEGGIDWAWGELLAFGSLANSGRLVRLAGEDSRRGTFTQRHAVAIDPATAEEFNPLHELAQSKGNNGKFLVYNSALTEYAGMGFEYGYSVGNEDSIVAWEAQFGDFANGAQTIIDEYVSSGEAKWGQTSKLILLLPHGYEGQGPDHSSARIERFLQLCAEGSMTVAQPSTPANHFHLLRRHALSDLKRPLVIFTPKSMLRNKAAASAPEDFTEVTKFQSVINDPNVADAAKVKKVMLVSGKLYYELAKRKEKDGRDDIAIVRIEMLHPIPFNRISEALAGYPNAEEVLFVQDEPANQGPWPFYQEHLPELIPNMPKMRRVSRRAQSSTATGVAKVHQLEEKQLIDEAFEA
InterPro domains: - IPR029061: Thiamin diphosphate-binding fold (homologous_superfamily) [405-1077]
- IPR031717: 2-oxoglutarate dehydrogenase E1 component/KDG, C-terminal (domain) [1077-1219]
- IPR032106: 2-oxoglutarate dehydrogenase E1 component, N-terminal domain (domain) [6-38]
- IPR001017: Dehydrogenase, E1 component (domain) [490-814]
- IPR001078: 2-oxoacid dehydrogenase acyltransferase, catalytic domain (domain) [104-337]
- IPR023213: Chloramphenicol acetyltransferase-like domain superfamily (homologous_superfamily) [90-339]
- IPR042179: Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain superfamily (homologous_superfamily) [1009-1218]
- IPR005475: Transketolase-like, pyrimidine-binding domain (domain) [876-1072]
- IPR011603: 2-oxoglutarate dehydrogenase E1 component (family) [1-1221]
Interaction partners: - Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex
- Dihydrolipoamide dehydrogenase/glutathione oxidoreductase and related enzymes
- Oxoglutarate dehydrogenase inhibitor
- Dihydrolipoamide dehydrogenase/glutathione oxidoreductase and related enzymes
- Dihydrolipoyl dehydrogenase
- Dihydrolipoamide dehydrogenase/glutathione oxidoreductase and related enzymes
- Succinate--CoA ligase subunit alpha
- FHA domain-containing protein
- FHA-domain-containing proteins
- Succinate--CoA ligase subunit beta
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0016740 (transferase activity), GO:1901681 (sulfur compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0019842 (vitamin binding), GO:0043168 (anion binding), GO:0043169 (cation binding), GO:0016744 (transketolase or transaldolase activity), GO:0046872 (metal ion binding), GO:0000287 (magnesium ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006099 (tricarboxylic acid cycle), GO:0006091 (generation of precursor metabolites and energy), GO:0006082 (organic acid metabolic process), GO:0015980 (energy derivation by oxidation of organic compounds), GO:0043436 (oxoacid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0045333 (cellular respiration), GO:0009060 (aerobic respiration), GO:0043648 (dicarboxylic acid metabolic process), GO:0006105 (succinate metabolic process), GO:0006103 (2-oxoglutarate metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0005622 (intracellular anatomical structure), GO:0005737 (cytoplasm), GO:1990204 (oxidoreductase complex), GO:0045254 (pyruvate dehydrogenase complex)