Protein: Protein prickle (Protein spiny legs) (A1Z6W3)
Organism: Drosophila melanogaster (Fruit fly)
Function: Acts in a planar cell polarity (PCP) complex; polarization along the apical/basal axis of epithelial cells. Correct expression of the alternative isoforms is required for PCP signaling in imaginal disks. PCP signaling in the wing disk requires the receptor fz and the cytoplasmic proteins dsh and pk. These act in a feedback loop leading to activation of the jnk cascade and subsequent polarized arrangement of hairs and bristles. Dgo and pk compete with one another for dsh binding, thereby modulating fz dsh activity and ensuring tight control over fz PCP signaling. Vang, stan and pk function together to regulate the establishment of tissue polarity in the adult eye.
Subcellular location: Cell membrane {ECO:0000269|PubMed:12015986, ECO:0000269|PubMed:12642492, ECO:0000269|PubMed:12941693, ECO:0000269|PubMed:15937478}; Peripheral membrane protein {ECO:0000269|PubMed:12015986, ECO:0000269|PubMed:12642492, ECO:0000269|PubMed:12941693, ECO:0000269|PubMed:15937478}; Cytoplasmic side {ECO:0000269|PubMed:12015986, ECO:0000269|PubMed:12642492, ECO:0000269|PubMed:12941693, ECO:0000269|PubMed:15937478}. Note=Localized to the proximal wing cell boundary where fz and dsh localization is antagonized by binding the dsh DEP domain and preventing dsh cortical localization. Localization to the anterior photoreceptor cell membrane. {ECO:0000269|PubMed:12015986, ECO:0000269|PubMed:12642492, ECO:0000269|PubMed:12941693, ECO:0000269|PubMed:15937478}.
Sequence: MSSLSTGGGAGGSSGGPGGADAAAAPAAGQATVTATGNMEPAMVPRTANLLACKQWWRVCFLYGDQQKYYRQLYSKAAAQRLADANQEPDNARDREYDTVDCDLIAGQLDAVEDADDGIDLGDHSSTPKGGATTAGRPLFPHSSSPRRSKKLLRSLRAHVRGEKLPKNDTTTANESSEVTQRNARVTVLDDPFLFGIDADHLGDLVVRGKRYSTLDATENMARFYAEQEATAQVLEIIEQEEESPEQEAPKPALPPKQKQQRPVPPLPPPPANRVTQDQGTQPAAPQVPLQPLTAGDLQFLNLSLRQRSLPRSMKPFKDAHDISFTFNELDTSAEPEVATGAAQQESNEPISRTPLTQISYLQKIPTLPRHFSPSGQGLATPPALGSGGMGLPSSSSASALYAAQAAAGILPTSPLPLQRHQQYLPPHHQQHPGAGMGPGPGSGAAAGPPLGPQYSPGCSANPKYSNAQLPPPPHHHHQLSPALSTPSPPSLLHHPAGGTSSASAHAPFLGGPHMDMQRQSHSDDDSGCALEEYTWVPPGLRPDQVRLYFSQIPDDKVPYVNSPGEQYRVRQLLHQLPPHDNEVRYCHSLTDEERKELRLFSTQRKRDALGRGNVRQLMSARPCDGCDDLISTGDIAVFATRLGPNASWHPACFACSVCRELLVDLIYFHRDGRMYCGRHHAETLKPRCSACDEIILADECTEAEGRAWHMNHFACHECDKQLGGQRYIMREGKPYCLHCFDAMFAEYCDYCGEAIGVDQGQMSHDGQHWHATDECFSCNTCRCSLLGRAFLPRRGAIYCSIACSKGEPPTPSDSSGTGMYTTPTPPTQRVRPHPQAPLPARIPSSHASSSPPMSPQQQQQHQATFNQAMYQMQSQQMEAAGGLVDQSKSYAASDSDAGVVKDLEHGGHMGGGDLTDFSGGRASSTSQNLSPLNSPGDFQPHFLPKPMELQRDGVYNFNEMSSNLDAAWSAKPTNSYHLQRQLLENPHTASMPELAGKLVAPPAHMQHLSQLHAVSSHQFQQHEYADILHPPPPPPGEIPELPTPNLSVASTALPPELMGSPTHSAGDRSLNTPMSTQSASHAPPHPVSILSGASSSSPMSGEPAKKKGVRFEGIPDTLPRSRSYSGNGAGTSGGGERERDRDKDKEGGGRHGHGHSSRRRRRRKSSSSSSHHRSGSGHRSHSTTRADTYAPAQPLSSSYQGPPSVLQAANLVHESPSRQQREREREREREESEESDVCSTCSSSSSSSEDYMMMYQLPQRRHYGGVRVSYVPNDALAYDRKRKPSELGGDKDKNCIIS
InterPro domains: - IPR033726: LIM2 prickle (domain) [687-742]
- IPR033727: LIM3 prickle (domain) [747-805]
- IPR033725: LIM1 prickle (domain) [624-682]
- IPR010442: PET domain (domain) [515-623]
- IPR033723: PET prickle (domain) [523-618]
- IPR047120: Prickle/Espinas/Testin (family) [446-818]
- IPR001781: Zinc finger, LIM-type (domain) [622-803]
Interaction partners: - Diego, isoform A
- Segment polarity protein dishevelled
- Vang-like protein
- Frizzled
- Frizzled-3
- Protocadherin-like wing polarity protein stan
- Dishevelled associated activator of morphogenesis, isoform D
- Protein espinas
- Rho-associated protein kinase
- Protein inturned
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0008037 (cell recognition), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0008038 (neuron recognition), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0048731 (system development), GO:0022008 (neurogenesis), GO:0007399 (nervous system development), GO:0030182 (neuron differentiation), GO:0048666 (neuron development), GO:0070593 (dendrite self-avoidance), GO:0048699 (generation of neurons)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)