Protein: Amyrin synthase LUP2 (EC 5.4.99.39) (EC 5.4.99.40) (EC 5.4.99.41) (Alpha-amyrin synthase) (Beta-amyrin synthase) (Lupeol synthase 2) (AtLUP2) (Q8RWT0)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Multifunctional enzyme that converts oxidosqualene to nine different triterpenes, mainly lupeol, beta-amyrin and alpha-amyrin in a 15:50:30 ratio.
Sequence: MWKLKIGEGNGEDPYLFSSNNFVGRQTWEFDPKAGTPEERAAVEDARRNYLDNRPRVKGCSDLLWRMQFLKEAKFEQVIPPVKIDDGEGITYKNATDALRRAVSFYSALQSSDGHWPAEITGTLFFLPPLVFCFYITGHLEKIFDAEHRKEMLRHIYCHQNEDGGWGLHIEGKSVMFCTVLNYICLRMLGEGPNGGRNNACKRARQWILDHGGVTYIPSWGKIWLSILGIYDWSGTNPMPPEIWLLPSFFPIHLGKTLCYTRMVYMPMSYLYGKRFVGPLTPLIMLLRKELHLQPYEEINWNKARRLCAKEDMIYPHPLVQDLLWDTLHNFVEPILTNWPLKKLVREKALRVAMEHIHYEDENSHYITIGCVEKVLCMLACWIENPNGDHFKKHLARIPDFMWVAEDGLKMQSFGSQLWDTVFAIQALLACDLSDETDDVLRKGHSFIKKSQVRENPSGDFKSMYRHISKGAWTLSDRDHGWQVSDCTAEALKCCMLLSMMPAEVVGQKIDPEQLYDSVNLLLSLQGEKGGLTAWEPVRAQEWLELLNPTDFFTCVMAEREYVECTSAVIQALVLFKQLYPDHRTKEIIKSIEKGVQFIESKQTPDGSWHGNWGICFIYATWFALSGLAAAGKTYKSCLAVRKGVDFLLAIQEEDGGWGESHLSCPEQRYIPLEGNRSNLVQTAWAMMGLIHAGQAERDPTPLHRAAKLIITSQLENGDFPQQEILGVFMNTCMLHYATYRNIFPLWALAEYRKAAFATHQDL
InterPro domains: - IPR008930: Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid (homologous_superfamily) [123-756]
- IPR032697: Squalene cyclase, N-terminal (domain) [100-403]
- IPR032696: Squalene cyclase, C-terminal (domain) [416-754]
- IPR018333: Squalene cyclase (family) [1-755]
- IPR002365: Terpene synthase, conserved site (conserved_site) [606-620]
Interaction partners: - Squalene epoxidase 6
- Squalene epoxidase 5
- Squalene epoxidase 3
- Squalene epoxidase 2, mitochondrial
- Squalene epoxidase 1
- Squalene epoxidase 4
- Hydroxymethylglutaryl-CoA synthase
- Tirucalladienol synthase
- Baruol synthase
- Beta-amyrin synthase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016853 (isomerase activity), GO:0016866 (intramolecular transferase activity), GO:0031559 (oxidosqualene cyclase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0006720 (isoprenoid metabolic process), GO:0008299 (isoprenoid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0006721 (terpenoid metabolic process), GO:0016114 (terpenoid biosynthetic process), GO:0006722 (triterpenoid metabolic process), GO:0016104 (triterpenoid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)