Protein: Cell division control protein 42 homolog (EC 3.6.5.2) (G25K GTP-binding protein) (P60953)
Organism: Homo sapiens (Human)
Function: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Regulates cell migration. In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. In podocytes, facilitates filopodia and podosomes formation upon DOCK11-activation. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling (By similarity). Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups.
Subcellular location: Cell membrane {ECO:0000269|PubMed:22843693}; Lipid-anchor {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:15642749}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:15642749}. Midbody {ECO:0000269|PubMed:15642749}. Cell projection, dendrite {ECO:0000250|UniProtKB:P60766}. Note=Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase (PubMed:15642749). Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells (PubMed:15642749). Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1 (PubMed:15642749). Colocalizes with NEK6 in the centrosome (PubMed:20873783). In its active GTP-bound form localizes to the leading edge membrane of migrating dendritic cells (By similarity). {ECO:0000250|UniProtKB:P60766, ECO:0000269|PubMed:15642749, ECO:0000269|PubMed:20873783}.
Sequence: MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL
InterPro domains: - IPR003578: Small GTPase Rho (family) [3-189]
- IPR005225: Small GTP-binding domain (domain) [1-157]
- IPR037874: Cdc42 (family) [3-177]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [1-181]
- IPR001806: Small GTPase (family) [1-191]
Interaction partners: - Rho guanine nucleotide exchange factor 7
- Rho GTPase-activating protein 1
- Intersectin-1
- Activated CDC42 kinase 1
- Ras GTPase-activating-like protein IQGAP2
- Actin nucleation-promoting factor WASL
- Partitioning defective 6 homolog beta
- Ras GTPase-activating-like protein IQGAP1
- Serine/threonine-protein kinase PAK 1
- Serine/threonine-protein kinase PAK 6
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0060090 (molecular adaptor activity), GO:0097367 (carbohydrate derivative binding), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0044877 (protein-containing complex binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0019899 (enzyme binding), GO:0032553 (ribonucleotide binding), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0019900 (kinase binding), GO:0032555 (purine ribonucleotide binding), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0017076 (purine nucleotide binding), GO:0016462 (pyrophosphatase activity), GO:0005525 (GTP binding), GO:0019001 (guanyl nucleotide binding), GO:0032561 (guanyl ribonucleotide binding), GO:0019901 (protein kinase binding), GO:0017111 (ribonucleoside triphosphate phosphatase activity), GO:0003924 (GTPase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0051641 (cellular localization), GO:0048856 (anatomical structure development), GO:0009653 (anatomical structure morphogenesis), GO:0048870 (cell motility), GO:0002684 (positive regulation of immune system process), GO:0071840 (cellular component organization or biogenesis), GO:0051241 (negative regulation of multicellular organismal process), GO:0051239 (regulation of multicellular organismal process), GO:0009892 (negative regulation of metabolic process), GO:0065008 (regulation of biological quality), GO:0040017 (positive regulation of locomotion), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0040013 (negative regulation of locomotion), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0048523 (negative regulation of cellular process), GO:0007165 (signal transduction), GO:0040012 (regulation of locomotion), GO:0023056 (positive regulation of signaling), GO:0007275 (multicellular organism development), GO:0048584 (positive regulation of response to stimulus), GO:0019222 (regulation of metabolic process), GO:0007155 (cell adhesion), GO:0032879 (regulation of localization), GO:0002682 (regulation of immune system process), GO:0009893 (positive regulation of metabolic process), GO:0030029 (actin filament-based process), GO:0006950 (response to stress), GO:0007154 (cell communication), GO:0048522 (positive regulation of cellular process), GO:0032103 (positive regulation of response to external stimulus), GO:0044085 (cellular component biogenesis), GO:2000145 (regulation of cell motility), GO:0002685 (regulation of leukocyte migration), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0009966 (regulation of signal transduction), GO:0030155 (regulation of cell adhesion), GO:0060255 (regulation of macromolecule metabolic process), GO:0032101 (regulation of response to external stimulus), GO:0060627 (regulation of vesicle-mediated transport), GO:0032886 (regulation of microtubule-based process), GO:0001817 (regulation of cytokine production), GO:0051668 (localization within membrane), GO:0032970 (regulation of actin filament-based process), GO:0044089 (positive regulation of cellular component biogenesis), GO:0044087 (regulation of cellular component biogenesis), GO:0010632 (regulation of epithelial cell migration), GO:0060263 (regulation of respiratory burst), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0007186 (G protein-coupled receptor signaling pathway), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:0007166 (cell surface receptor signaling pathway), GO:0051128 (regulation of cellular component organization), GO:0090066 (regulation of anatomical structure size), GO:0031589 (cell-substrate adhesion), GO:0016477 (cell migration), GO:2000146 (negative regulation of cell motility), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0009967 (positive regulation of signal transduction), GO:0051049 (regulation of transport), GO:0035556 (intracellular signal transduction), GO:0009611 (response to wounding), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0050920 (regulation of chemotaxis), GO:0006952 (defense response), GO:0051130 (positive regulation of cellular component organization), GO:0045785 (positive regulation of cell adhesion), GO:0030036 (actin cytoskeleton organization), GO:2000147 (positive regulation of cell motility), GO:0002687 (positive regulation of leukocyte migration), GO:0045806 (negative regulation of endocytosis), GO:0031325 (positive regulation of cellular metabolic process), GO:0010646 (regulation of cell communication), GO:0031323 (regulation of cellular metabolic process), GO:0001818 (negative regulation of cytokine production), GO:0010647 (positive regulation of cell communication), GO:0080090 (regulation of primary metabolic process), GO:0050921 (positive regulation of chemotaxis), GO:0032535 (regulation of cellular component size), GO:0043254 (regulation of protein-containing complex assembly), GO:0030030 (cell projection organization), GO:0051246 (regulation of protein metabolic process), GO:0010810 (regulation of cell-substrate adhesion), GO:0032956 (regulation of actin cytoskeleton organization), GO:0030334 (regulation of cell migration), GO:1902622 (regulation of neutrophil migration), GO:0150116 (regulation of cell-substrate junction organization), GO:0060491 (regulation of cell projection assembly), GO:0010468 (regulation of gene expression), GO:0034446 (substrate adhesion-dependent cell spreading), GO:0022607 (cellular component assembly), GO:1902903 (regulation of supramolecular fiber organization), GO:0032233 (positive regulation of actin filament bundle assembly), GO:0070507 (regulation of microtubule cytoskeleton organization), GO:0010594 (regulation of endothelial cell migration), GO:0032231 (regulation of actin filament bundle assembly), GO:1902905 (positive regulation of supramolecular fiber organization), GO:0120034 (positive regulation of plasma membrane bounded cell projection assembly), GO:1902533 (positive regulation of intracellular signal transduction), GO:0030335 (positive regulation of cell migration), GO:0043933 (protein-containing complex organization), GO:0002688 (regulation of leukocyte chemotaxis), GO:0007264 (small GTPase mediated signal transduction), GO:0010629 (negative regulation of gene expression), GO:0150117 (positive regulation of cell-substrate junction organization), GO:0007015 (actin filament organization), GO:1901890 (positive regulation of cell junction assembly), GO:0010811 (positive regulation of cell-substrate adhesion), GO:0007229 (integrin-mediated signaling pathway), GO:0010562 (positive regulation of phosphorus metabolic process), GO:0007167 (enzyme-linked receptor protein signaling pathway), GO:0031344 (regulation of cell projection organization), GO:2000377 (regulation of reactive oxygen species metabolic process), GO:1901888 (regulation of cell junction assembly), GO:1902624 (positive regulation of neutrophil migration), GO:0002690 (positive regulation of leukocyte chemotaxis), GO:0030336 (negative regulation of cell migration), GO:0006954 (inflammatory response), GO:0031346 (positive regulation of cell projection organization), GO:0010634 (positive regulation of epithelial cell migration), GO:0051247 (positive regulation of protein metabolic process), GO:0033043 (regulation of organelle organization), GO:0006996 (organelle organization), GO:0010638 (positive regulation of organelle organization), GO:0097435 (supramolecular fiber organization), GO:0051174 (regulation of phosphorus metabolic process), GO:1902531 (regulation of intracellular signal transduction), GO:0031334 (positive regulation of protein-containing complex assembly), GO:0120032 (regulation of plasma membrane bounded cell projection assembly), GO:0051493 (regulation of cytoskeleton organization), GO:0031116 (positive regulation of microtubule polymerization), GO:1900026 (positive regulation of substrate adhesion-dependent cell spreading), GO:0032271 (regulation of protein polymerization), GO:0065003 (protein-containing complex assembly), GO:0031113 (regulation of microtubule polymerization), GO:0045937 (positive regulation of phosphate metabolic process), GO:0001952 (regulation of cell-matrix adhesion), GO:0008154 (actin polymerization or depolymerization), GO:0051057 (positive regulation of small GTPase mediated signal transduction), GO:0007010 (cytoskeleton organization), GO:0110020 (regulation of actomyosin structure organization), GO:0051894 (positive regulation of focal adhesion assembly), GO:0051496 (positive regulation of stress fiber assembly), GO:0007169 (transmembrane receptor protein tyrosine kinase signaling pathway), GO:0001954 (positive regulation of cell-matrix adhesion), GO:0031110 (regulation of microtubule polymerization or depolymerization), GO:0031112 (positive regulation of microtubule polymerization or depolymerization), GO:0010592 (positive regulation of lamellipodium assembly), GO:0071624 (positive regulation of granulocyte chemotaxis), GO:0031401 (positive regulation of protein modification process), GO:0090023 (positive regulation of neutrophil chemotaxis), GO:1900024 (regulation of substrate adhesion-dependent cell spreading), GO:0048259 (regulation of receptor-mediated endocytosis), GO:0090022 (regulation of neutrophil chemotaxis), GO:0120035 (regulation of plasma membrane bounded cell projection organization), GO:0031399 (regulation of protein modification process), GO:0030031 (cell projection assembly), GO:0120036 (plasma membrane bounded cell projection organization), GO:0071622 (regulation of granulocyte chemotaxis), GO:0010595 (positive regulation of endothelial cell migration), GO:0008361 (regulation of cell size), GO:0019220 (regulation of phosphate metabolic process), GO:1902745 (positive regulation of lamellipodium organization), GO:0090109 (regulation of cell-substrate junction assembly), GO:0051056 (regulation of small GTPase mediated signal transduction), GO:0010762 (regulation of fibroblast migration), GO:0110053 (regulation of actin filament organization), GO:0051492 (regulation of stress fiber assembly), GO:0032273 (positive regulation of protein polymerization), GO:0051495 (positive regulation of cytoskeleton organization), GO:0001932 (regulation of protein phosphorylation), GO:0046578 (regulation of Ras protein signal transduction), GO:1902743 (regulation of lamellipodium organization), GO:0010591 (regulation of lamellipodium assembly), GO:0120031 (plasma membrane bounded cell projection assembly), GO:0031529 (ruffle organization), GO:0042327 (positive regulation of phosphorylation), GO:0051893 (regulation of focal adhesion assembly), GO:0001934 (positive regulation of protein phosphorylation), GO:0046579 (positive regulation of Ras protein signal transduction), GO:0030041 (actin filament polymerization), GO:0042325 (regulation of phosphorylation), GO:0097581 (lamellipodium organization), GO:0051258 (protein polymerization), GO:0030032 (lamellipodium assembly), GO:0035023 (regulation of Rho protein signal transduction)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0098552 (side of membrane), GO:0030054 (cell junction), GO:0031984 (organelle subcompartment), GO:0031256 (leading edge membrane), GO:0098794 (postsynapse), GO:0019898 (extrinsic component of membrane), GO:0099080 (supramolecular complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0071944 (cell periphery), GO:0031252 (cell leading edge), GO:0005615 (extracellular space), GO:0036477 (somatodendritic compartment), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005576 (extracellular region), GO:0120025 (plasma membrane bounded cell projection), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0045202 (synapse), GO:0001726 (ruffle), GO:0098791 (Golgi apparatus subcompartment), GO:0005794 (Golgi apparatus), GO:0035770 (ribonucleoprotein granule), GO:0070161 (anchoring junction), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0099081 (supramolecular polymer), GO:0031090 (organelle membrane), GO:0036464 (cytoplasmic ribonucleoprotein granule), GO:0019897 (extrinsic component of plasma membrane), GO:0030141 (secretory granule), GO:0030027 (lamellipodium), GO:0098562 (cytoplasmic side of membrane), GO:0043230 (extracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0043197 (dendritic spine), GO:0098590 (plasma membrane region), GO:0005783 (endoplasmic reticulum), GO:0097447 (dendritic tree), GO:0032587 (ruffle membrane), GO:0070062 (extracellular exosome), GO:0055037 (recycling endosome), GO:0031253 (cell projection membrane), GO:0099503 (secretory vesicle), GO:0031234 (extrinsic component of cytoplasmic side of plasma membrane), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0098978 (glutamatergic synapse), GO:0005802 (trans-Golgi network), GO:0030425 (dendrite), GO:0070820 (tertiary granule), GO:0099512 (supramolecular fiber), GO:0030659 (cytoplasmic vesicle membrane), GO:0030055 (cell-substrate junction), GO:0101002 (ficolin-1-rich granule), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0012506 (vesicle membrane), GO:0009898 (cytoplasmic side of plasma membrane), GO:0065010 (extracellular membrane-bounded organelle), GO:0043005 (neuron projection), GO:0030667 (secretory granule membrane), GO:0005789 (endoplasmic reticulum membrane), GO:0010008 (endosome membrane), GO:0031982 (vesicle), GO:0005925 (focal adhesion), GO:1903561 (extracellular vesicle), GO:0005856 (cytoskeleton), GO:0044309 (neuron spine), GO:0099513 (polymeric cytoskeletal fiber), GO:0097708 (intracellular vesicle), GO:0005634 (nucleus), GO:0055038 (recycling endosome membrane), GO:0101003 (ficolin-1-rich granule membrane), GO:0005884 (actin filament), GO:0015629 (actin cytoskeleton)