Protein: Oocyte-expressed protein homolog (Factor located in oocytes permitting embryonic development) (Floped) (Oocyte- and embryo-specific protein 19) (mOEP19) (STAT3 downstream gene and differentiation regulator) (Q9CWE6)
Organism: Mus musculus (Mouse)
Function: As part of the OOEP-KHDC3 scaffold, recruits BLM and TRIM25 to DNA replication forks, thereby promoting the ubiquitination of BLM by TRIM25, enhancing BLM retainment at replication forks and therefore promoting stalled replication fork restart. Positively regulates the homologous recombination-mediated DNA double-strand break (DSB) repair pathway by regulating ATM activation and RAD51 recruitment to DSBs in oocytes. Thereby contributes to oocyte survival and the resumption and completion of meiosis. As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the formation of F-actin cytoplasmic lattices in oocytes which in turn are responsible for symmetric division of zygotes via the regulation of mitotic spindle formation and positioning.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:18804437}. Nucleus {ECO:0000269|PubMed:29125140}. Note=In the subcortical cytoplasm of early embryos from the 1-cell to the blastocyst stages (PubMed:18804437, PubMed:25208553). From the 2-cell stage, still detected in the subcortex, but excluded from cell-cell contact regions (PubMed:18804437). Expression largely disappears in blastocysts (PubMed:25208553). {ECO:0000269|PubMed:18804437, ECO:0000269|PubMed:25208553}.
Sequence: MASHTADADAKPDSDSQKLLNVLPVSLRLRTRPWWFPIQEVSNPLVLYMEAWVAERVIGTDQAEISEIEWMCQALLTVDSVNSGNLAEITIFGQPSAQTRMKNILLNMAAWHKENELQRAVKVKEVEEFLKIRASSILSKLSKKGLKLAGFPLPLEGRETQMES
InterPro domains: - IPR051778: KH Domain-Containing Protein 1 (family) [4-133]
- IPR036612: K Homology domain, type 1 superfamily (homologous_superfamily) [15-127]
- IPR031952: KH-like RNA-binding domain (domain) [23-128]
Interaction partners: - KH domain-containing protein 3
- Transducin-like enhancer protein 6
- NACHT, LRR and PYD domains-containing protein 5
- Inactive protein-arginine deiminase type-6
- Zinc finger BED domain-containing protein 3
- NACHT
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003677 (DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0009893 (positive regulation of metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0030154 (cell differentiation), GO:0046483 (heterocycle metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010628 (positive regulation of gene expression), GO:0010467 (gene expression), GO:0010468 (regulation of gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0008380 (RNA splicing), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0000398 (mRNA splicing, via spliceosome), GO:0000375 (RNA splicing, via transesterification reactions), GO:0000380 (alternative mRNA splicing, via spliceosome), GO:0000377 (RNA splicing, via transesterification reactions with bulged adenosine as nucleophile)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)