Protein: Sorbitol dehydrogenase (SDH) (EC 1.1.1.-) (L-iditol 2-dehydrogenase) (EC 1.1.1.14) (Polyol dehydrogenase) (Xylitol dehydrogenase) (XDH) (EC 1.1.1.9) (Q58D31)
Organism: Bos taurus (Bovine)
Function: Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols. Is mostly active with xylitol, D-sorbitol (D-glucitol) and L-iditol as substrates, leading to the C2-oxidized products D-xylulose, D-fructose and L-sorbose, respectively. Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility (By similarity). Cannot use NADP(+) as the electron acceptor. Has no activity on ethanol, methanol, glycerol, galactitol and fructose 6-phosphate.
Subcellular location: Mitochondrion membrane {ECO:0000250|UniProtKB:Q64442}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q64442}. Cell projection, cilium, flagellum {ECO:0000250|UniProtKB:Q64442}. Note=Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface. {ECO:0000250|UniProtKB:Q64442}.
Sequence: MAAAKPENLSLVVHGPGDLRLENYPIPEPGPNEVLLKMHSVGICGSDVHYWQHGRIGDFVVKKPMVLGHEASGTVVKVGSLVRHLQPGDRVAIEPGAPRETDEFCKIGRYNLSPTIFFCATPPDDGNLCRFYKHNANFCYKLPDNVTFEEGALIEPLSVGIHACRRAGVTLGNKVLVCGAGPIGLVSLLAAKAMGAAQVVVTDLSASRLSKAKEVGADFILQISNESPQEIAKKVEGLLGSKPEVTIECTGVETSIQAGIYATHSGGTLVLVGLGSEMTSVPLVHAATREVDIKGVFRYCNTWPMAISMLASKSVNVKPLVTHRFPLEKALEAFETSKKGLGLKVMIKCDPNDQNP
InterPro domains: - IPR036291: NAD(P)-binding domain superfamily (homologous_superfamily) [145-315]
- IPR020843: Enoylreductase domain (domain) [15-347]
- IPR045306: Sorbitol dehydrogenase-like (family) [10-349]
- IPR013149: Alcohol dehydrogenase-like, C-terminal (domain) [182-311]
- IPR011032: GroES-like superfamily (homologous_superfamily) [9-180]
- IPR013154: Alcohol dehydrogenase-like, N-terminal (domain) [30-144]
- IPR002328: Alcohol dehydrogenase, zinc-type, conserved site (conserved_site) [68-82]
Interaction partners: - NADP-dependent oxidoreductase domain-containing protein
- Aldo-keto reductase family 1 member B1
- Xylulose kinase
- hexokinase
- Hexokinase-1
- Hexokinase-2
- Ketohexokinase
- Aldo-keto reductase family 1 member B10
- L-xylulose reductase
- Hydroxyacid-oxoacid transhydrogenase, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0016491 (oxidoreductase activity), GO:0016614 (oxidoreductase activity, acting on CH-OH group of donors), GO:1901265 (nucleoside phosphate binding), GO:0000166 (nucleotide binding), GO:0043169 (cation binding), GO:0016616 (oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor), GO:0046872 (metal ion binding), GO:0017076 (purine nucleotide binding), GO:0046914 (transition metal ion binding), GO:0030554 (adenyl nucleotide binding), GO:0008270 (zinc ion binding), GO:0051287 (NAD binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044282 (small molecule catabolic process), GO:0044283 (small molecule biosynthetic process), GO:0044248 (cellular catabolic process), GO:0006066 (alcohol metabolic process), GO:0005996 (monosaccharide metabolic process), GO:0005975 (carbohydrate metabolic process), GO:1901575 (organic substance catabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0044275 (cellular carbohydrate catabolic process), GO:0019318 (hexose metabolic process), GO:1901616 (organic hydroxy compound catabolic process), GO:0046164 (alcohol catabolic process), GO:0046364 (monosaccharide biosynthetic process), GO:0019400 (alditol metabolic process), GO:0019751 (polyol metabolic process), GO:0016051 (carbohydrate biosynthetic process), GO:0016052 (carbohydrate catabolic process), GO:0006059 (hexitol metabolic process), GO:0006006 (glucose metabolic process), GO:0019405 (alditol catabolic process), GO:0019319 (hexose biosynthetic process), GO:0046174 (polyol catabolic process), GO:0006000 (fructose metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex)