Protein: Ribonuclease III domain-containing protein RNC1, chloroplastic (Chloroplast ribonuclease III domain protein) (Q9SZV0)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Binds specific group II introns in chloroplasts and facilitates their splicing. Acts on both subgroup IIA and subgroup IIB introns. The substrates of the subgroup II also require the CRM domain proteins CAF1 or CAF2. Binds both single-stranded and double-stranded RNA non-specifically, but lacks endonuclease activity. Required for plastid ribosome biogenesis.
Subcellular location: Plastid, chloroplast {ECO:0000255}.
Sequence: MELCSSSPSSSLLRICSSSAPEISFSSSISQFPSKTQSILTKSRFQNLRICASVTAETQGLPRDSPQRLLKELAQRKTATGPKKKVPPKRFILRPPLDDKKLAERFLNSPQLSLKSFPLLSSCLPSSKLNNADKTWIDEYLLEVKQALGYSLEPSESLGDDNPAKHFDTLLYLAFQHPSCDRARARHVKNGHSRLWFLGQYVLELALTEFFLQRYPRESPGPMRERVFALIGKRYLPKWIKAASLQNLIFPYDDMDKLIRKEREPPVKSVFWALFGAIYLCFGMPEVYRVLFEVFGMDPDADECQPRSRRQLEDVDYVSVEFEGKKLGWQDIATYKPPEDALFAHPRLFRACVPPGMHRFRGNIWDFDSKPKVMQTLGYPLTMNDRIKEITEARNIELGLGLQLCFLHPSKHKFEHPRFCFERLEYVGQKIQDIAMAERLLMKHLDAPGKWLQEKHRRLLMNKFCGRYLREKRLHNFIIYSEEVHDRYEHNRRLRNPATTAVQQAIHGLAYTIYGKPDVRRLMFEVFDFEQIQPKAV
InterPro domains: - IPR036389: Ribonuclease III, endonuclease domain superfamily (homologous_superfamily) [137-530]
- IPR000999: Ribonuclease III domain (domain) [141-527]
Interaction partners: - Protein WHAT'S THIS FACTOR 1 homolog
- CRS2-associated factor 2, chloroplastic
- Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic
- Protein RETICULATA-RELATED 6
- CRM-domain containing factor CFM2, chloroplastic
- Protein THYLAKOID ASSEMBLY 8, chloroplastic
- Pentatricopeptide repeat-containing protein At5g04810, chloroplastic
- GTP-binding protein At2g22870
- Pentatricopeptide repeat-containing protein At5g42310, chloroplastic
- Putative nucleobase-ascorbate transporter 9
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0003729 (mRNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0042254 (ribosome biogenesis), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0034470 (ncRNA processing), GO:0006364 (rRNA processing), GO:0034660 (ncRNA metabolic process), GO:0016072 (rRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0009532 (plastid stroma), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009570 (chloroplast stroma), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle)