Protein: Ran GTPase-activating protein 1 (RanGAP1) (P46061)
Organism: Mus musculus (Mouse)
Function: GTPase activator for RAN. Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export. Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export (By similarity). Required for postimplantation embryonic development.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:26506250, ECO:0000269|PubMed:9442102, ECO:0000269|PubMed:9456312}. Nucleus, nucleoplasm {ECO:0000269|PubMed:26506250}. Nucleus envelope {ECO:0000269|PubMed:16469311, ECO:0000269|PubMed:18305100, ECO:0000269|PubMed:26506250, ECO:0000269|PubMed:9442102, ECO:0000269|PubMed:9456312}. Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:P46060}. Cytoplasm, cytoskeleton, spindle {ECO:0000250|UniProtKB:P46060}. Note=Cytoplasmic during interphase (PubMed:26506250). Detected at the nuclear envelope during interphase (PubMed:26506250, PubMed:9442102, PubMed:9456312). Shuttles between nucleus and cytoplasm (PubMed:26506250). Targeted to the nuclear pores after sumoylation. During mitosis, associates with mitotic spindles, but is essentially not detected at the spindle poles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation (By similarity). {ECO:0000250|UniProtKB:P46060, ECO:0000269|PubMed:26506250, ECO:0000269|PubMed:9442102, ECO:0000269|PubMed:9456312}.
Sequence: MASEDIAKLAETLAKTQVAGGQLSFKGKGLKLNTAEDAKDVIKEIEEFDGLEALRLEGNTVGVEAARVIAKALEKKSELKRCHWSDMFTGRLRSEIPPALISLGEGLITAGAQLVELDLSDNAFGPDGVRGFEALLKSPACFTLQELKLNNCGMGIGGGKILAAALTECHRKSSAQGKPLALKVFVAGRNRLENDGATALAEAFGIIGTLEEVHMPQNGINHPGVTALAQAFAINPLLRVINLNDNTFTEKGGVAMAETLKTLRQVEVINFGDCLVRSKGAVAIADAVRGGLPKLKELNLSFCEIKRDAALVVAEAVADKAELEKLDLNGNALGEEGCEQLQEVMDSFNMAKVLASLSDDEGEDEDEEEEGEEDDEEEEDEEDEEDDDEEEEEQEEEEEPPQRGSGEEPATPSRKILDPNSGEPAPVLSSPTPTDLSTFLSFPSPEKLLRLGPKVSVLIVQQTDTSDPEKVVSAFLKVASVFRDDASVKTAVLDAIDALMKKAFSCSSFNSNTFLTRLLIHMGLLKSEDKIKAIPSLHGPLMVLNHVVRQDYFPKALAPLLLAFVTKPNGALETCSFARHNLLQTLYNI
InterPro domains: - IPR032675: Leucine-rich repeat domain superfamily (homologous_superfamily) [21-365]
- IPR009109: Ran-GTPase activating protein 1, C-terminal (domain) [413-586]
- IPR027038: Ran GTPase-activating protein (family) [1-542]
- IPR036720: Ran-GTPase activating protein 1, C-terminal domain superfamily (homologous_superfamily) [419-589]
- IPR001611: Leucine-rich repeat (repeat) [114-339]
Interaction partners: - SUMO-conjugating enzyme UBC9
- E3 SUMO-protein ligase RanBP2
- Ran-specific GTPase-activating protein
- Small ubiquitin-related modifier 1
- GTP-binding nuclear protein Ran
- Small ubiquitin-related modifier 2
- Small ubiquitin-related modifier 3
- Regulator of chromosome condensation
- Sentrin-specific protease 1
- Exportin-1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0005488 (binding), GO:0030234 (enzyme regulator activity), GO:0140677 (molecular function activator activity), GO:0005515 (protein binding), GO:0019899 (enzyme binding), GO:0008047 (enzyme activator activity), GO:0060589 (nucleoside-triphosphatase regulator activity), GO:0051020 (GTPase binding), GO:0030695 (GTPase regulator activity), GO:0005096 (GTPase activator activity), GO:0031267 (small GTPase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0048519 (negative regulation of biological process), GO:0051641 (cellular localization), GO:0051651 (maintenance of location in cell), GO:0051051 (negative regulation of transport), GO:0032879 (regulation of localization), GO:0051235 (maintenance of location), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0051234 (establishment of localization), GO:0051716 (cellular response to stimulus), GO:0065009 (regulation of molecular function), GO:0071704 (organic substance metabolic process), GO:0048523 (negative regulation of cellular process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0033036 (macromolecule localization), GO:0050790 (regulation of catalytic activity), GO:0044093 (positive regulation of molecular function), GO:0051049 (regulation of transport), GO:1901564 (organonitrogen compound metabolic process), GO:0046907 (intracellular transport), GO:1903828 (negative regulation of protein localization), GO:0060341 (regulation of cellular localization), GO:0051224 (negative regulation of protein transport), GO:0019538 (protein metabolic process), GO:0006810 (transport), GO:0032507 (maintenance of protein location in cell), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0043170 (macromolecule metabolic process), GO:0045185 (maintenance of protein location), GO:0032387 (negative regulation of intracellular transport), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0051223 (regulation of protein transport), GO:0051336 (regulation of hydrolase activity), GO:0070201 (regulation of establishment of protein localization), GO:0072595 (maintenance of protein localization in organelle), GO:0051169 (nuclear transport), GO:0032880 (regulation of protein localization), GO:0008104 (protein localization), GO:0043085 (positive regulation of catalytic activity), GO:0090317 (negative regulation of intracellular protein transport), GO:1904950 (negative regulation of establishment of protein localization), GO:0046823 (negative regulation of nucleocytoplasmic transport), GO:0032386 (regulation of intracellular transport), GO:0006913 (nucleocytoplasmic transport), GO:0043087 (regulation of GTPase activity), GO:0033365 (protein localization to organelle), GO:0051457 (maintenance of protein location in nucleus), GO:0051345 (positive regulation of hydrolase activity), GO:0070647 (protein modification by small protein conjugation or removal), GO:0033157 (regulation of intracellular protein transport), GO:0018193 (peptidyl-amino acid modification), GO:0046822 (regulation of nucleocytoplasmic transport), GO:0034504 (protein localization to nucleus), GO:0051168 (nuclear export), GO:0046825 (regulation of protein export from nucleus), GO:0018205 (peptidyl-lysine modification), GO:0032446 (protein modification by small protein conjugation), GO:0043547 (positive regulation of GTPase activity), GO:0016925 (protein sumoylation), GO:0090630 (activation of GTPase activity)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0005829 (cytosol), GO:0048471 (perinuclear region of cytoplasm), GO:0031975 (envelope), GO:0036477 (somatodendritic compartment), GO:0140513 (nuclear protein-containing complex), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031967 (organelle envelope), GO:0120025 (plasma membrane bounded cell projection), GO:0005643 (nuclear pore), GO:0043229 (intracellular organelle), GO:0099568 (cytoplasmic region), GO:0043228 (non-membrane-bounded organelle), GO:0005635 (nuclear envelope), GO:0097447 (dendritic tree), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0030425 (dendrite), GO:0043005 (neuron projection), GO:0032838 (plasma membrane bounded cell projection cytoplasm), GO:0005634 (nucleus), GO:0005856 (cytoskeleton), GO:0005819 (spindle), GO:0120111 (neuron projection cytoplasm), GO:0030424 (axon), GO:0072686 (mitotic spindle), GO:0015630 (microtubule cytoskeleton), GO:1904115 (axon cytoplasm)