Protein: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (EC 7.1.1.2) (Complex I-75kD) (CI-75kD) (P28331)
Organism: Homo sapiens (Human)
Function: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for catalysing the entry and efficient transfer of electrons within complex I. Plays a key role in the assembly and stability of complex I and participates in the association of complex I with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes.
Subcellular location: Mitochondrion inner membrane {ECO:0000305|PubMed:12611891, ECO:0000305|PubMed:30879903}; Peripheral membrane protein {ECO:0000250|UniProtKB:P15690}; Matrix side {ECO:0000250|UniProtKB:P15690}.
Sequence: MLRIPVRKALVGLSKSPKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGNDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTYTSWEDALSRVAGMLQSFQGKDVAAIAGGLVDAEALVALKDLLNRVDSDTLCTEEVFPTAGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYTYDHLGDSPKILQDIASGSHPFSQVLKEAKKPMVVLGSSALQRNDGAAILAAVSSIAQKIRMTSGVTGDWKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKVLFLLGADGGCITRQDLPKDCFIIYQGHHGDVGAPIADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGMTLPYDTLDQVRNRLEEVSPNLVRYDDIEGANYFQQANELSKLVNQQLLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAQAVEEPSIC
InterPro domains: - IPR050123: Prokaryotic molybdopterin-containing oxidoreductase (family) [98-717]
- IPR036010: 2Fe-2S ferredoxin-like superfamily (homologous_superfamily) [32-104]
- IPR001041: 2Fe-2S ferredoxin-type iron-sulfur binding domain (domain) [30-108]
- IPR006963: Molybdopterin oxidoreductase, 4Fe-4S domain (domain) [245-301]
- IPR000283: NADH:ubiquinone oxidoreductase, 75kDa subunit, conserved site (conserved_site) [61-185]
- IPR054351: NADH-ubiquinone oxidoreductase, ferredoxin-like domain (domain) [166-232]
- IPR006656: Molybdopterin oxidoreductase (domain) [301-628]
- IPR010228: NADH:ubiquinone oxidoreductase, subunit G (family) [34-626]
- IPR019574: NADH:ubiquinone oxidoreductase, subunit G, iron-sulphur binding (domain) [108-153]
- IPR015405: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial-like (domain) [658-710]
Interaction partners: - NADH dehydrogenase iron-sulfur protein 4, mitochondrial
- NADH dehydrogenase 1 alpha subcomplex subunit 7
- NADH dehydrogenase 1 beta subcomplex subunit 7
- NADH dehydrogenase 1 alpha subcomplex subunit 11
- NADH dehydrogenase 1 alpha subcomplex subunit 1
- Cytochrome c1, heme protein, mitochondrial
- NADH dehydrogenase flavoprotein 1, mitochondrial
- NADH dehydrogenase iron-sulfur protein 3, mitochondrial
- NADH-ubiquinone oxidoreductase chain 1
- NADH dehydrogenase 1 beta subcomplex subunit 9
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity), GO:0005515 (protein binding), GO:0016491 (oxidoreductase activity), GO:0009055 (electron transfer activity), GO:0016651 (oxidoreductase activity, acting on NAD(P)H), GO:0022804 (active transmembrane transporter activity), GO:0008137 (NADH dehydrogenase (ubiquinone) activity), GO:0003954 (NADH dehydrogenase activity), GO:0015399 (primary active transmembrane transporter activity), GO:0016655 (oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor), GO:0015453 (oxidoreduction-driven active transmembrane transporter activity), GO:0050136 (NADH dehydrogenase (quinone) activity), GO:0003955 (NAD(P)H dehydrogenase (quinone) activity)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0016043 (cellular component organization), GO:0044085 (cellular component biogenesis), GO:0006996 (organelle organization), GO:0043933 (protein-containing complex organization), GO:0022607 (cellular component assembly), GO:0065003 (protein-containing complex assembly), GO:0007005 (mitochondrion organization), GO:0033108 (mitochondrial respiratory chain complex assembly), GO:0010257 (NADH dehydrogenase complex assembly), GO:0032981 (mitochondrial respiratory chain complex I assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)