Protein: Terpene cyclase/mutase family member (A4FVM4)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Key enzyme in the cholesterol biosynthesis pathway. Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. Through the production of lanosterol may regulate lens protein aggregation and increase transparency
Subcellular location: Endoplasmic reticulum membrane; Peripheral membrane protein
Sequence: MTEGTCLRRRGGPYKTEPATDLSRWRLSNVDGRQSWRYIEETDSLDRPQSMLERHSLGLDTSEFISASPAAHTAVEAALKGMDFYSRLQAEDGHWAGDYGGPLFLLPGLLITCHIAKIPLPDAWKKEMVRYLRSVQLPDGGWGLHIEDKSTVFGTALSYTTLRILGVGPDDPDMVRARNALHNRGGAVGIPSWGKFWLAILNVYSWEGMNTLFPEMWLLPSWMPAHPSTLWCHCRQVYLPMSYCYAVRLSADEDPLVLSLRQELYVQDYSTIDWPAQRNNVATCDLYTPHSNLLTFAYFFLNVYEAHHSTILREKAVKELYDHIKADDRFTKCISIGPISKTINMLVRWYVDGPTSPAFQKHVSRIPDYLWLGLDGMKMQGTNGSQLWDTAFAVQAFLEAGAQDIPRFTECLTQAHHFLDLTQVKDNPPEYEKYYRQMNKGGFPFSTRDCGWIVADCVSEGLKSVMLLQEQCNFLKENIPKERLFDAVNVLLSMRNPDGGFATYETKRGGKLLELLNPSEVFGDIMIDYTYVECTSAVLQALKHFHSVYPEHRAEEIRSTLQRGLDYCRRVQRPDGSWEGSWGVCFTYGAWFGLEAFACMGHTFQNGSVCEEVKRACEFLLAKQMEDGGWGEDFESCEQRRYVQSSSSQIHNTCWALLGLMAVRYPGTKVIERGIQLLIDKQLPNGDWPQENISGVFNKSCAISYTSYRNVFPVWTLGRFTRLYPCNALTGKLKL
InterPro domains: - IPR002365: Terpene synthase, conserved site (conserved_site) [575-589]
- IPR008930: Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid (homologous_superfamily) [101-725]
- IPR018333: Squalene cyclase (family) [62-722]
- IPR032696: Squalene cyclase, C-terminal (domain) [385-721]
- IPR032697: Squalene cyclase, N-terminal (domain) [84-326]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016853 (isomerase activity), GO:0016866 (intramolecular transferase activity), GO:0031559 (oxidosqualene cyclase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0048732 (gland development), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006720 (isoprenoid metabolic process), GO:0008299 (isoprenoid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0008202 (steroid metabolic process), GO:0006721 (terpenoid metabolic process), GO:0016114 (terpenoid biosynthetic process), GO:0006722 (triterpenoid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005811 (lipid droplet)