Protein: Membrane metallo-endopeptidase-like 1 (EC 3.4.24.11) (NEP2(m)) (Neprilysin II) (NEPII) (Neprilysin-2) (NEP2) (NL2) (Neprilysin-like 1) (NL-1) (Neprilysin-like peptidase) (NEPLP) (Soluble secreted endopeptidase) [Cleaved into: Membrane metallo-endopeptidase-like 1, soluble form (Neprilysin-2 secreted) (NEP2(s))] (Q9JLI3)
Organism: Mus musculus (Mouse)
Function: Metalloprotease involved in sperm function, possibly by modulating the processes of fertilization and early embryonic development. Degrades a broad variety of small peptides with a preference for peptides shorter than 3 kDa containing neutral bulky aliphatic or aromatic amino acid residues. Shares the same substrate specificity with MME and cleaves peptides at the same amide bond.
Subcellular location: Membrane {ECO:0000269|PubMed:10542292}; Single-pass type II membrane protein {ECO:0000269|PubMed:10542292}. Secreted {ECO:0000269|PubMed:10542292}. Note=A secreted form produced by proteolytic cleavage also exists.
Sequence: MVERAGWCRKKSPGFVEYGLMVLLLLLLGAIVTLGVFYSIGKQLPLLTSLLHFSWDERTVVKRALRDSSLKSDICTTPSCVIAAARILENMDQSRNPCENFYQYACGGWLRHHVIPETNSRYSVFDILRDELEVILKGVLEDSTSQHRPAVEKAKTLYRSCMNQSVIEKRDSEPLLSVLKMVGGWPVAMDKWNETMGLKWELERQLAVLNSQFNRRVLIDLFIWNDDQNSSRHVIYIDQPTLGMPSREYYFQEDNNHKVRKAYLEFMTSVATMLRKDQNLSKESAMVREEMAEVLELETHLANATVPQEKRHDVTALYHRMDLMELQERFGLKGFNWTLFIQNVLSSVEVELFPDEEVVVYGIPYLENLEDIIDSYSARTMQNYLVWRLVLDRIGSLSQRFKEARVDYRKALYGTTVEEVRWRECVSYVNSNMESAVGSLYIKRAFSKDSKSTVRELIEKIRSVFVDNLDELNWMDEESKKKAQEKAMNIREQIGYPDYILEDNNKHLDEEYSSLTFYEDLYFENGLQNLKNNAQRSLKKLREKVDQNLWIIGAAVVNAFYSPNRNQIVFPAGILQPPFFSKDQPQSLNFGGIGMVIGHEITHGFDDNGRNFDKNGNMLDWWSNFSARHFQQQSQCMIYQYGNFSWELADNQNVNGFSTLGENIADNGGVRQAYKAYLRWLADGGKDQRLPGLNLTYAQLFFINYAQVWCGSYRPEFAVQSIKTDVHSPLKYRVLGSLQNLPGFSEAFHCPRGSPMHPMKRCRIW
InterPro domains: - IPR000718: Peptidase M13 (family) [23-765]
- IPR018497: Peptidase M13, C-terminal domain (domain) [550-764]
- IPR008753: Peptidase M13, N-terminal domain (domain) [97-497]
- IPR042089: Peptidase M13, domain 2 (homologous_superfamily) [123-545]
- IPR024079: Metallopeptidase, catalytic domain superfamily (homologous_superfamily) [75-765]
Interaction partners: - Insulin-degrading enzyme
- Amyloid-beta precursor protein
- Beta-secretase 1
- Angiotensin-converting enzyme
- Prolow-density lipoprotein receptor-related protein 1
- Angiotensinogen
- Presenilin-1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0008233 (peptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0032501 (multicellular organismal process), GO:0065008 (regulation of biological quality), GO:0023051 (regulation of signaling), GO:0023056 (positive regulation of signaling), GO:0071704 (organic substance metabolic process), GO:0003008 (system process), GO:0006807 (nitrogen compound metabolic process), GO:0097242 (amyloid-beta clearance), GO:0050794 (regulation of cellular process), GO:0048522 (positive regulation of cellular process), GO:0010647 (positive regulation of cell communication), GO:0048167 (regulation of synaptic plasticity), GO:0050877 (nervous system process), GO:0099177 (regulation of trans-synaptic signaling), GO:0043603 (amide metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0050806 (positive regulation of synaptic transmission), GO:0010646 (regulation of cell communication), GO:0050804 (modulation of chemical synaptic transmission), GO:1900273 (positive regulation of long-term synaptic potentiation), GO:0006518 (peptide metabolic process), GO:1900271 (regulation of long-term synaptic potentiation), GO:0007600 (sensory perception), GO:0050435 (amyloid-beta metabolic process), GO:0019233 (sensory perception of pain)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0030141 (secretory granule), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0030667 (secretory granule membrane), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0031982 (vesicle), GO:0012506 (vesicle membrane), GO:0030659 (cytoplasmic vesicle membrane), GO:0097708 (intracellular vesicle)