Protein: Non-reducing polyketide synthase ZEA1 (A0A098D6U0)
Organism: Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084
Function: Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of zearalenone (ZEA), a nonsteroid estrogen that is a contaminant of cereal grains and causes estrogenic disorders in humans and animals. The ZEA backbone is synthesized from a single acetyl- CoA molecule and eight malonyl-CoA molecules. The reducing polyketide synthase ZEA2 is proposed to synthesize a reduced hexaketide intermediate by using different combinations of its reductive domains during each round of condensation. The hexaketide thioester is then transacylated to the non-reducing polyketide synthase ZEA1 and is further condensed with three malonyl-CoAs without reductive tailoring to yield a mixed reduced/unreduced nonaketide. ZEA1 must be able to interact with ZEA2 to facilitate starter-unit acyltransfer and initiate polyketide biosynthesis. ZEA1 also mediates the required C2-C7 cyclization to form the resorcylate core and catalyzes the formation of the macrolactone. ZEA1 exhibits broad starter-unit specificities toward fatty acyl-CoAs ranging in sizes between C6 and C16 and displays the highest activity toward decanoyl-CoA. ZEB1 is then responsible for the chemical conversion of beta-zearalenonol (beta-ZOL) to ZEA in the biosynthetic pathway
Sequence: MAPNKKTILLFGDQTDSWVDGIDRLYQDAASIPWLQSFLDDLTHTFKTHTVGMDAVLRNSLGDFANLQELAEKYRYTTDDVGMAQAFLIYAVRAGILLKWAKYEPSLLSTDENQPEWVGISGGLISLSVLAVAETFEKLYEACLEVAGLLARLCRFTSVKSRSMEDRSGAWGWTVLGIGANELRNALDQYQQSMGIPPIKRAQVAVTGHRWNTIVGPPSILQLIVKECPAIRSLPKNELNIHALQHTVVTSRADLDYIVGDSTLLSQPLSLTFTLWGMDDPRAHYTTWGDMLRAICSQALSRPLDITHVVDQLSSKLRSFPQLDVKSIGPCSHLSYLTNVLKSAGRTVSVADDHPPSTPPKQLPGRIAIVGMAGRGPGSDNVEEFWNVIMSKLDLCEEIPEDRFNLSEFYRSKHDSGCTTTTKFGCFMDKPGHFDNRFFHISPREALLMDPGHRQFLMTTYEALEMAGYSDGATRAVDPARIATFFGQCNDDWHDVSHHTLGCDAYTLQGVQRAFGAGRIAFQFKWEGPTYSLDSACASTASSIHLACTSLLAKETDMAVAGAANVVGYPHSWTSLSKSGVLSDTGNCKTFRDDADGYCRADFVGTVVLKRLEDAIAHNDNILAVVAASGRNHSGNSSSITTSDAKAQEKLYRKMMHNARVSPNDISYVEMHGTGTKVGDPAEMGALASLFSHRRTPKPVVVGGVKANVGHSESKQAAGVASLLKCIMMFQKNILPPQAGMPHALNPNFPPLSEINIEIPSEPSTFESPVSQPRRILLNNFDAAGGNACILLEDFGNNMVKKSDPRVHHTVVTSSRTQASYHGNKAKLLQWLRQNPGARIEDVAYTTTARRTHHPIRFAVTASSTQELISKLEADTADSPAAKESPVVFVFTGQGSHYAGMGSELYETSPVFRETVNLCATICEEQNFPPFLDLITQSDSEMSDKTTLEVQLAVLTLEIGLAALWRSIGIQPSVVIGHSLGEYAALHVSGVLSLADVLYLVGQRALLILQRCEVNTSAMLSVAMPVTDTHAFLEAQADPSCEIACVNSTNASVISGSIENITELQAGLKARSKMLSVPYGFHSSQMDPILADYAALAGGVTFSEPKIPVASTLLASLVDTSGTFHAGYMARQCRQPVNFVGALEAIQSNYSDPIWLEIGPSQVCSSFVRATLSPPPSKILSTLDKGTNAWLSLGKCMSSLYKHGATIDWLALHQPYVDNLSLLNLPTYAWDLKEFWIRYTETKDQLPTSTTNGHETFKANISTCAQQVVEHISPPNMKVTFRASLSDPGFKALIDGHRLRDRSVCPGSVFSEAGLAAVTYVLQLHHSKSLKLPALVLRNLSLKRPLTYDLVGPDGELITVVAPGGSSNDTFKVAWKASKGNVSYSLGDCMVAACDGQLIQARWDKVSYFIRSRVDEIVASSKSGISHRLQPQILYGLFANTVKYDAAFKCIQEAYISSDFQEAAAVIVLNSDPVGTKFVASPYWGESLVHLAGFVVNSNPSRQSHDTTFMMDGFESFEQTVIPEPGKPYHTYVRVTGNESASVVCDVYIFDDDKLIMHCAGLHFHEVENAVLDQLLGGTNTSNTTRDQPAPIMSRKEVPKPVDTTEKVEAVSNDSQSDAHVLDSILKIISKETGSDLADFQDDTLIADLGVDSIMAIEIASQVTEETGMDLLPSFIIDYPAIGDLRRVFAPKSTHISLDNDLSRPSLVDDTSQALQSSGSESFDQPPTSVTSTSDSGSIVKIDLGPDVDSPAPKIKITLLQGRPGNGRTPFYLIADGTGTIATYIHLPQFKSQIPIYGIDSPFLRCPTRFTTDVGITGAARFITEALMKAQPEGTFVLGGFSGGAMLAYEVCRQLAAANRKVDSLMLIDMCSPRSKTVEDKNDIGWAIFESISRQNGLWRSTDMTRQHLQAIFAAVATYHPQPLKASERPKRTAIIWAEKGMIDRCAGDSELMQKLAKRGIPTEPYPKFMEDSELGPVAWGLPHKTKNDLGPNGWERYVGDALCLSMPADHLEMPMPGHVHLLHEKMTRAFEFFNEAG
InterPro domains: - IPR006162: Phosphopantetheine attachment site (ptm) [1648-1663]
- IPR014043: Acyl transferase domain (domain) [888-1187]
- IPR032088: Starter unit:ACP transacylase, SAT domain (domain) [9-246]
- IPR036736: ACP-like superfamily (homologous_superfamily) [1619-1692]
- IPR016035: Acyl transferase/acyl hydrolase/lysophospholipase (homologous_superfamily) [887-1184]
- IPR001227: Acyl transferase domain superfamily (homologous_superfamily) [886-1185]
- IPR020841: Polyketide synthase, beta-ketoacyl synthase domain (domain) [364-797]
- IPR016039: Thiolase-like (homologous_superfamily) [364-802]
- IPR001031: Thioesterase (domain) [1823-1883]
- IPR049551: Polyketide synthase, dehydratase domain, C-terminal (domain) [1423-1572]
- IPR018201: Beta-ketoacyl synthase, active site (active_site) [528-544]
- IPR042104: Polyketide synthase, dehydratase domain superfamily (homologous_superfamily) [1259-1577]
- IPR030918: Polyketide product template domain (domain) [1262-1577]
- IPR014031: Beta-ketoacyl synthase, C-terminal (domain) [623-739]
- IPR016036: Malonyl-CoA ACP transacylase, ACP-binding (homologous_superfamily) [1016-1073]
- IPR009081: Phosphopantetheine binding ACP domain (domain) [1616-1693]
- IPR020806: Polyketide synthase-like, phosphopantetheine-binding domain (domain) [1622-1693]
- IPR029058: Alpha/Beta hydrolase fold (homologous_superfamily) [1752-2031]
- IPR014030: Beta-ketoacyl synthase-like, N-terminal (domain) [366-615]
- IPR049900: Polyketide synthase, dehydratase domain (domain) [1254-1573]
Interaction partners: - Chromosome 1, complete genome
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0042802 (identical protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:1901615 (organic hydroxy compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0009404 (toxin metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0018904 (ether metabolic process), GO:0030638 (polyketide metabolic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:1901503 (ether biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0043385 (mycotoxin metabolic process), GO:0009403 (toxin biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0030639 (polyketide biosynthetic process), GO:0018958 (phenol-containing compound metabolic process), GO:0043386 (mycotoxin biosynthetic process), GO:0046189 (phenol-containing compound biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:1990204 (oxidoreductase complex), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)