Protein: Serine/threonine-protein kinase MEC1 (Q59LR2)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]- Q. Recruited to DNA lesions in order to initiate the DNA repair by homologous recombination. Phosphorylates histone H2A to form H2AS128ph (gamma-H2A) at sites of DNA damage, also involved in the regulation of DNA damage response mechanism. Required for cell growth and meiotic recombination (By similarity)
Subcellular location: Nucleus. Note=Localizes to nuclear DNA repair foci in response to DNA double strand breaks
Sequence: MTSNQSISTTELLQFLTDIETNIDDHETDFRKLLLYLLRFTNEKLIVIAQEENKTPTELQLLSKLIDTIELVLSKKTPLLSTLLTIEDVNIIHTTGSGSLVYEVPLHEWCISFALSHIPNFVSHTAGLNQLKRLVFLIVNLVSTQLHSFKVIKSTRIHLLKTLDDNLNFCLQNLLSANTFLFKSKLTTAVNLFSIVHDYDISQKLSLNLNNYQLKFESCSRKIWFILNEISLVSELDNLNLLDCLKSVFILDQSSALVLNVSVGWNQIGFLLSCIVEYLQQDFRTLDSNTNNFEFVNLNRSISLSLLNVYIVCFDKDLLENFMSFSNIKGILSKLIYDDSIPSVIRKTLNIVQYTYQLMSNPDGDDIKLYSTSVYNDYVWTPFVDSELESLRARLLDLQGNHEDSRKEELLSFSIDETHKLAKSTNSLNYTDEKAWIRTVKKLIGIDKNVLEDETTLYTLVTALSHYPCILKGDYDYTINECTKCGFGPLTKNNYSSIDPNRFPLNYSTEATTLQDIIQQFLIPKLETQQDPLLCCNTLLLIFNFYASFSPMADMQDHNILDFLLRLLATNDNRDVRMLVARILPLYLIQSKDDKLLDETFKYIFQKVTSIDFSSQHRLHFGESTIRAVVELATVSTGERLCAIYFKLVDWLGEQNEQHSNYVYCGILNLASAKSLPPHKLLSPYLPSVAEIIIKKPQVFERIIKVSMVTKNYFLNRTKEYTVPRILEYYKDPTLLTQIANAAGLEVGKLLANCLPRILATYLTKESVNERYIMKVLSSVCPDYKMIHTEELFTRIGDITWYILLEIQMDEFGNIRNLANITRALECVCKNVSLRKNGSELTKNNSINDLIEDQVLLLVQKFSDVTHSSRGAKPYLELKNSFYAIEFLIKGHIDAITSALGQLSTCLQATLEEPNFHVLSLRCWNELIKKVPPSHLISLIDIIISIILQKFESFGSEAKSIAIEILRKIYEEIKDKYNRYSLYFLSLPFLSYMEDYQMVKEFRNMKSPSRAMIFSEFTRRLQTSNMYVVKHALFDLSNYFEKYQINCQKDLFKDPGLTPAITSLVRTILDTAAKFKNKDTTVSTACAKALAIIGALDSNKFQFKTVKSLIIISSDFEDIEENSTFLVDLIENHLLKIFWASNDPHKQLFAAYVMQSFLAVMGLDERVLNTKDNRVWNKFTDVAKSTLTPFLKSKYAAPKPKLDNLKFPFFKLGMKYETWLVDVTLFLLKRASIDNGKGNQKAKTRKLIFQSYAVLIQREHDIPLCEHLLRYVALSHVINEGVPEDLHKEFLHILKMDSKSTSPDRAEQLKLCYQTIFSVLDYFNQWVSNMRVVTSNSGSELTSSDIRHKMDAVAKFSSFPQDLLTTRSAECDAYERTIMYLENCYRDSQSEKSFKLSNLNGAATLQDMYAHIDDYDALNGTLKMFSTNNLNEKLTTFQYSDSWSLAHESFEALGSTKNSVSNNTKLLQSLNEHGLYNEVLSTLSARTDSNDLKSIPLDWSLMGLHAAVYKGDSKQLEKWLQVINSIGKPHDMETMINYELAKALSFLFQSRIDMFKGSMDKLYNIIGCSLVPSVSSNFTRNITLMNQLHAIYDVSLIVLSKDSEDTLDLRIGNVDQDFDTQRNILTLHNVANTVMKNPAMISKNLLRESSLARKYNRLDISTRSIVQAMSLEDDQANIEFAELQWAQGKQSEAIKCLFDILKDNKFHDDKSKAKVQLQYANWLDESNHLSAHQIITEYNKAFHLNMVDEKCNFDIGKYYNKLMESSNDESGEYEHLTVRNYIRAVSVGTTYIFEALPKVLTIWLDFADKSNKSNAAENRLKQIIDDLYNAIANVPNYSWYTVLTQILSRIVHEHEPSFKVLKRIVQNVTLEYPKHCVWYIFSHARSSDKVRKRRVRELLNQVCTQDGNDTLPKSTMAAGNLFAKLIKIAELKIPKTNRKRQMSLLQDFEVDLSEPIDDLVIPIQSNLQIQIPSHLNSKHKGFSRSSSISFDGFDDNVNIFFSLQMPRQLTVRGSDGNAYRLMVKSDDTRKDAKVVEFTTMVNRILSTSTEARKRGLQIANYSVVPLSDHFGIIEFVMNVQTMKGVISEQRKRQGIPINERKVFMHIDSLQKAKKKDSKQLDKLVAGFRAIMDRCPPVLHTWFVEQFSDPSAWYMARNAFTRSSAVMSMVGYIMGLGDRHCENILIFKNTGAVLHIDFDCLFEKGTTLPTPEIVPFRLTQNMVDAMGITGVDGIYRITCEVTGTLLRENEQILMNILETLIYDPLIDWRNHNPREDLSKVRKKIRGLINEDEGLPMNIHGQVDVLIQEATSLERLSQMYAGWAAYM
InterPro domains: - IPR056802: Serine/threonine-protein kinase ATR-like, M-HEAT region (domain) [1204-1437]
- IPR050517: DNA Damage Response and Repair Kinase (family) [99-2324]
- IPR012993: UME domain (domain) [847-953]
- IPR016024: Armadillo-type fold (homologous_superfamily) [277-1102]
- IPR003152: FATC domain (domain) [2293-2325]
- IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [1969-2321]
- IPR014009: PIK-related kinase, FAT domain (domain) [1363-1886]
- IPR057564: Serine/threonine-protein kinase ATR-like, HEAT repeats (domain) [1822-1917]
- IPR036940: Phosphatidylinositol 3-/4-kinase, catalytic domain superfamily (homologous_superfamily) [2129-2322]
- IPR003151: PIK-related kinase, FAT (domain) [1605-1801]
- IPR000403: Phosphatidylinositol 3-/4-kinase, catalytic domain (domain) [1993-2324]
- IPR018936: Phosphatidylinositol 3-/4-kinase, conserved site (conserved_site) [2162-2182]
- IPR058681: Serine/threonine-protein kinase MEC1, HEAT repeats (domain) [24-731]
Interaction partners: - Serine/threonine-protein kinase RAD53
- Serine/threonine-protein kinase TEL1
- DNA damage checkpoint protein LCD1
- Checkpoint protein
- Chromatin-binding protein
- Checkpoint protein RAD24
- non-specific serine/threonine protein kinase
- Cyclin-dependent kinase 1
- DNA repair protein rad9
- Serine/threonine protein kinase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0035173 (histone kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0048519 (negative regulation of biological process), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0048523 (negative regulation of cellular process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0051726 (regulation of cell cycle), GO:1901360 (organic cyclic compound metabolic process), GO:0035556 (intracellular signal transduction), GO:0006139 (nucleobase-containing compound metabolic process), GO:0006979 (response to oxidative stress), GO:0046483 (heterocycle metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0045786 (negative regulation of cell cycle), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0010948 (negative regulation of cell cycle process), GO:0044260 (cellular macromolecule metabolic process), GO:0010564 (regulation of cell cycle process), GO:0043170 (macromolecule metabolic process), GO:0034599 (cellular response to oxidative stress), GO:0000075 (cell cycle checkpoint signaling), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0062197 (cellular response to chemical stress), GO:1901987 (regulation of cell cycle phase transition), GO:0006259 (DNA metabolic process), GO:1901988 (negative regulation of cell cycle phase transition), GO:0031570 (DNA integrity checkpoint signaling), GO:0006281 (DNA repair), GO:0000077 (DNA damage checkpoint signaling), GO:0000076 (DNA replication checkpoint signaling)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005694 (chromosome), GO:0005634 (nucleus), GO:0000228 (nuclear chromosome), GO:0000793 (condensed chromosome), GO:0000794 (condensed nuclear chromosome)