Protein: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99.-) (P16858)
Organism: Mus musculus (Mouse)
Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (By similarity). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
Subcellular location: Cytoplasm, cytosol {ECO:0000250|UniProtKB:P04797}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P04797}. Nucleus {ECO:0000250|UniProtKB:P04797}. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Colocalizes with CHP1 to small punctate structures along the microtubules tracks. {ECO:0000250|UniProtKB:P04797}.
Sequence: MVKVGVNGFGRIGRLVTRAAICSGKVEIVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQERDPTNIKWGEAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEDQVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEYGYSNRVVDLMAYMASKE
InterPro domains: - IPR036291: NAD(P)-binding domain superfamily (homologous_superfamily) [1-171]
- IPR020828: Glyceraldehyde 3-phosphate dehydrogenase, NAD(P) binding domain (domain) [2-150]
- IPR006424: Glyceraldehyde-3-phosphate dehydrogenase, type I (family) [3-324]
- IPR020831: Glyceraldehyde/Erythrose phosphate dehydrogenase family (family) [1-333]
- IPR020829: Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain (domain) [155-312]
- IPR020830: Glyceraldehyde 3-phosphate dehydrogenase, active site (active_site) [148-155]
Interaction partners: - Large ribosomal subunit protein uL13
- Bifunctional glutamate/proline--tRNA ligase
- Triosephosphate isomerase
- Heterogeneous nuclear ribonucleoprotein Q
- Heat shock cognate 71 kDa protein
- Phosphoglycerate kinase 1
- Fructose-bisphosphate aldolase A
- Phosphoglycerate kinase 2
- Glucose-6-phosphate isomerase
- Fructose-bisphosphate aldolase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0005515 (protein binding), GO:0016903 (oxidoreductase activity, acting on the aldehyde or oxo group of donors), GO:0016620 (oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor), GO:0043891 (glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity), GO:0004365 (glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0023056 (positive regulation of signaling), GO:0042221 (response to chemical), GO:0048584 (positive regulation of response to stimulus), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0009892 (negative regulation of metabolic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0009893 (positive regulation of metabolic process), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0065009 (regulation of molecular function), GO:0048523 (negative regulation of cellular process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0009967 (positive regulation of signal transduction), GO:0050790 (regulation of catalytic activity), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0044092 (negative regulation of molecular function), GO:0044248 (cellular catabolic process), GO:0019637 (organophosphate metabolic process), GO:0009966 (regulation of signal transduction), GO:0060255 (regulation of macromolecule metabolic process), GO:0005996 (monosaccharide metabolic process), GO:0005975 (carbohydrate metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0006793 (phosphorus metabolic process), GO:0009894 (regulation of catabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0010941 (regulation of cell death), GO:0006139 (nucleobase-containing compound metabolic process), GO:0010646 (regulation of cell communication), GO:0031325 (positive regulation of cellular metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0006082 (organic acid metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0010647 (positive regulation of cell communication), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:1901575 (organic substance catabolic process), GO:0006091 (generation of precursor metabolites and energy), GO:0043086 (negative regulation of catalytic activity), GO:0006753 (nucleoside phosphate metabolic process), GO:0019318 (hexose metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0006757 (ATP generation from ADP), GO:0051246 (regulation of protein metabolic process), GO:0071310 (cellular response to organic substance), GO:0043436 (oxoacid metabolic process), GO:0043067 (regulation of programmed cell death), GO:0051248 (negative regulation of protein metabolic process), GO:2001235 (positive regulation of apoptotic signaling pathway), GO:0006796 (phosphate-containing compound metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:0010468 (regulation of gene expression), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0050727 (regulation of inflammatory response), GO:0043620 (regulation of DNA-templated transcription in response to stress), GO:0031326 (regulation of cellular biosynthetic process), GO:0010563 (negative regulation of phosphorus metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0016052 (carbohydrate catabolic process), GO:0031329 (regulation of cellular catabolic process), GO:0051253 (negative regulation of RNA metabolic process), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0051174 (regulation of phosphorus metabolic process), GO:0006006 (glucose metabolic process), GO:0009141 (nucleoside triphosphate metabolic process), GO:0042981 (regulation of apoptotic process), GO:0051346 (negative regulation of hydrolase activity), GO:0030162 (regulation of proteolysis), GO:0031399 (regulation of protein modification process), GO:0031400 (negative regulation of protein modification process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0071345 (cellular response to cytokine stimulus), GO:0019220 (regulation of phosphate metabolic process), GO:0016310 (phosphorylation), GO:0009259 (ribonucleotide metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0006163 (purine nucleotide metabolic process), GO:1902680 (positive regulation of RNA biosynthetic process), GO:0043618 (regulation of transcription from RNA polymerase II promoter in response to stress), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0045936 (negative regulation of phosphate metabolic process), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0042325 (regulation of phosphorylation), GO:0001933 (negative regulation of protein phosphorylation), GO:0001932 (regulation of protein phosphorylation), GO:0045893 (positive regulation of DNA-templated transcription), GO:0046939 (nucleotide phosphorylation), GO:0009144 (purine nucleoside triphosphate metabolic process), GO:0009150 (purine ribonucleotide metabolic process), GO:0009199 (ribonucleoside triphosphate metabolic process), GO:0045892 (negative regulation of DNA-templated transcription), GO:0006165 (nucleoside diphosphate phosphorylation), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:0009135 (purine nucleoside diphosphate metabolic process), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:0032787 (monocarboxylic acid metabolic process), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0061615 (glycolytic process through fructose-6-phosphate), GO:0045944 (positive regulation of transcription by RNA polymerase II), GO:0046031 (ADP metabolic process), GO:0006090 (pyruvate metabolic process), GO:0046034 (ATP metabolic process), GO:0009205 (purine ribonucleoside triphosphate metabolic process), GO:0009179 (purine ribonucleoside diphosphate metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0031967 (organelle envelope), GO:0005739 (mitochondrion), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0005740 (mitochondrial envelope), GO:0019866 (organelle inner membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0031966 (mitochondrial membrane), GO:0005743 (mitochondrial inner membrane)