Protein: Vacuolar protein sorting-associated protein 4B (EC 3.6.4.6) (Cell migration-inducing gene 1 protein) (Suppressor of K(+) transport growth defect 1) (Protein SKD1) (O75351)
Organism: Homo sapiens (Human)
Function: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their ATP-dependent disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan.
Subcellular location: Late endosome membrane {ECO:0000250|UniProtKB:P46467}; Peripheral membrane protein {ECO:0000305}. Note=Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells. {ECO:0000269|PubMed:14505570}.
Sequence: MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG
InterPro domains: - IPR036181: MIT domain superfamily (homologous_superfamily) [2-94]
- IPR003960: ATPase, AAA-type, conserved site (conserved_site) [272-291]
- IPR007330: MIT domain (domain) [4-82]
- IPR003593: AAA+ ATPase domain (domain) [166-302]
- IPR041569: AAA ATPase, AAA+ lid domain (domain) [324-362]
- IPR003959: ATPase, AAA-type, core (domain) [170-300]
- IPR015415: Spastin/Vps4, C-terminal (domain) [381-441]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [129-433]
- IPR050304: Microtubule-severing AAA ATPase (family) [20-442]
- IPR045253: Vacuolar protein sorting-associated protein 4, MIT domain (domain) [6-81]
Interaction partners: - Vacuolar protein sorting-associated protein VTA1 homolog
- Charged multivesicular body protein 1a
- Charged multivesicular body protein 2b
- Charged multivesicular body protein 5
- Charged multivesicular body protein 2a
- Charged multivesicular body protein 1b
- Charged multivesicular body protein 6
- IST1 homolog
- Vacuolar protein sorting-associated protein 4A
- Programmed cell death 6-interacting protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0016887 (ATP hydrolysis activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0044877 (protein-containing complex binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:0005515 (protein binding), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0019904 (protein domain specific binding), GO:0032553 (ribonucleotide binding), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0032555 (purine ribonucleotide binding), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0017076 (purine nucleotide binding), GO:0016462 (pyrophosphatase activity), GO:0005524 (ATP binding), GO:0032559 (adenyl ribonucleotide binding), GO:0030554 (adenyl nucleotide binding), GO:0017111 (ribonucleoside triphosphate phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0008152 (metabolic process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0048519 (negative regulation of biological process), GO:0016032 (viral process), GO:0051641 (cellular localization), GO:0048856 (anatomical structure development), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0051301 (cell division), GO:0071840 (cellular component organization or biogenesis), GO:0051050 (positive regulation of transport), GO:0009838 (abscission), GO:0019076 (viral release from host cell), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0048523 (negative regulation of cellular process), GO:0046755 (viral budding), GO:0007165 (signal transduction), GO:0051640 (organelle localization), GO:0007275 (multicellular organism development), GO:0048524 (positive regulation of viral process), GO:0032879 (regulation of localization), GO:0007049 (cell cycle), GO:0051234 (establishment of localization), GO:0071704 (organic substance metabolic process), GO:0044403 (biological process involved in symbiotic interaction), GO:0009056 (catabolic process), GO:0033036 (macromolecule localization), GO:0019068 (virion assembly), GO:0044237 (cellular metabolic process), GO:0019058 (viral life cycle), GO:0050792 (regulation of viral process), GO:0030029 (actin filament-based process), GO:0007154 (cell communication), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0051302 (regulation of cell division), GO:0046761 (viral budding from plasma membrane), GO:0000910 (cytokinesis), GO:0051047 (positive regulation of secretion), GO:0044085 (cellular component biogenesis), GO:0044248 (cellular catabolic process), GO:0046753 (non-lytic viral release), GO:0060627 (regulation of vesicle-mediated transport), GO:0010948 (negative regulation of cell cycle process), GO:0044089 (positive regulation of cellular component biogenesis), GO:0045184 (establishment of protein localization), GO:0044087 (regulation of cellular component biogenesis), GO:0010876 (lipid localization), GO:0051701 (biological process involved in interaction with host), GO:0051726 (regulation of cell cycle), GO:0046907 (intracellular transport), GO:0044837 (actomyosin contractile ring organization), GO:0051656 (establishment of organelle localization), GO:0016043 (cellular component organization), GO:0045786 (negative regulation of cell cycle), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0010564 (regulation of cell cycle process), GO:1903902 (positive regulation of viral life cycle), GO:0032506 (cytokinetic process), GO:1903530 (regulation of secretion by cell), GO:0051049 (regulation of transport), GO:1901564 (organonitrogen compound metabolic process), GO:1903047 (mitotic cell cycle process), GO:0035556 (intracellular signal transduction), GO:0060341 (regulation of cellular localization), GO:0019538 (protein metabolic process), GO:0039702 (viral budding via host ESCRT complex), GO:0051649 (establishment of localization in cell), GO:0051782 (negative regulation of cell division), GO:0030036 (actin cytoskeleton organization), GO:0043170 (macromolecule metabolic process), GO:0007059 (chromosome segregation), GO:1903900 (regulation of viral life cycle), GO:1903532 (positive regulation of secretion by cell), GO:0006810 (transport), GO:0050000 (chromosome localization), GO:0070727 (cellular macromolecule localization), GO:0000278 (mitotic cell cycle), GO:0007346 (regulation of mitotic cell cycle), GO:0071705 (nitrogen compound transport), GO:0030163 (protein catabolic process), GO:0044000 (movement in host), GO:0032365 (intracellular lipid transport), GO:0071702 (organic substance transport), GO:0032465 (regulation of cytokinesis), GO:0007093 (mitotic cell cycle checkpoint signaling), GO:0022607 (cellular component assembly), GO:0009057 (macromolecule catabolic process), GO:0006886 (intracellular protein transport), GO:0061640 (cytoskeleton-dependent cytokinesis), GO:1902410 (mitotic cytokinetic process), GO:0032466 (negative regulation of cytokinesis), GO:0015031 (protein transport), GO:0045930 (negative regulation of mitotic cell cycle), GO:0007080 (mitotic metaphase plate congression), GO:0043933 (protein-containing complex organization), GO:0022411 (cellular component disassembly), GO:0061024 (membrane organization), GO:0098813 (nuclear chromosome segregation), GO:0036213 (contractile ring contraction), GO:0000075 (cell cycle checkpoint signaling), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0010256 (endomembrane system organization), GO:0072594 (establishment of protein localization to organelle), GO:0140014 (mitotic nuclear division), GO:0017157 (regulation of exocytosis), GO:0032880 (regulation of protein localization), GO:0045921 (positive regulation of exocytosis), GO:0051303 (establishment of chromosome localization), GO:0006869 (lipid transport), GO:0016197 (endosomal transport), GO:0000916 (actomyosin contractile ring contraction), GO:0030866 (cortical actin cytoskeleton organization), GO:0044265 (cellular macromolecule catabolic process), GO:1901987 (regulation of cell cycle phase transition), GO:0044091 (membrane biogenesis), GO:0000281 (mitotic cytokinesis), GO:0016192 (vesicle-mediated transport), GO:0051046 (regulation of secretion), GO:0008104 (protein localization), GO:0007034 (vacuolar transport), GO:0006996 (organelle organization), GO:0000070 (mitotic sister chromatid segregation), GO:0031032 (actomyosin structure organization), GO:0101024 (mitotic nuclear membrane organization), GO:1901988 (negative regulation of cell cycle phase transition), GO:1902750 (negative regulation of cell cycle G2/M phase transition), GO:0015850 (organic hydroxy compound transport), GO:0072666 (establishment of protein localization to vacuole), GO:1905475 (regulation of protein localization to membrane), GO:0016050 (vesicle organization), GO:0015918 (sterol transport), GO:0007010 (cytoskeleton organization), GO:1902749 (regulation of cell cycle G2/M phase transition), GO:0006605 (protein targeting), GO:0051310 (metaphase plate congression), GO:0071709 (membrane assembly), GO:0007041 (lysosomal transport), GO:0061025 (membrane fusion), GO:0006900 (vesicle budding from membrane), GO:0033365 (protein localization to organelle), GO:1901991 (negative regulation of mitotic cell cycle phase transition), GO:0006998 (nuclear envelope organization), GO:0048285 (organelle fission), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0051276 (chromosome organization), GO:0051603 (proteolysis involved in protein catabolic process), GO:0071763 (nuclear membrane organization), GO:0006997 (nucleus organization), GO:1901990 (regulation of mitotic cell cycle phase transition), GO:0000819 (sister chromatid segregation), GO:0007084 (mitotic nuclear membrane reassembly), GO:0006906 (vesicle fusion), GO:0032984 (protein-containing complex disassembly), GO:0061952 (midbody abscission), GO:0032366 (intracellular sterol transport), GO:0035891 (exit from host cell), GO:1904375 (regulation of protein localization to cell periphery), GO:0006623 (protein targeting to vacuole), GO:0048284 (organelle fusion), GO:0032367 (intracellular cholesterol transport), GO:0010389 (regulation of G2/M transition of mitotic cell cycle), GO:0000280 (nuclear division), GO:1903076 (regulation of protein localization to plasma membrane), GO:0010972 (negative regulation of G2/M transition of mitotic cell cycle), GO:0030301 (cholesterol transport), GO:0019941 (modification-dependent protein catabolic process), GO:0072665 (protein localization to vacuole), GO:0031468 (nuclear membrane reassembly), GO:0006622 (protein targeting to lysosome), GO:0051261 (protein depolymerization), GO:0090174 (organelle membrane fusion), GO:0034058 (endosomal vesicle fusion), GO:0030865 (cortical cytoskeleton organization), GO:0006511 (ubiquitin-dependent protein catabolic process), GO:0061462 (protein localization to lysosome), GO:0043162 (ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005815 (microtubule organizing center), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0048471 (perinuclear region of cytoplasm), GO:0000922 (spindle pole), GO:0071944 (cell periphery), GO:0090543 (Flemming body), GO:0030496 (midbody), GO:0005615 (extracellular space), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005576 (extracellular region), GO:0031090 (organelle membrane), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043230 (extracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0005813 (centrosome), GO:0005773 (vacuole), GO:0070062 (extracellular exosome), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0000323 (lytic vacuole), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0012506 (vesicle membrane), GO:0005770 (late endosome), GO:0065010 (extracellular membrane-bounded organelle), GO:0005769 (early endosome), GO:0005774 (vacuolar membrane), GO:0010008 (endosome membrane), GO:0031982 (vesicle), GO:0030659 (cytoplasmic vesicle membrane), GO:0005764 (lysosome), GO:1903561 (extracellular vesicle), GO:0097708 (intracellular vesicle), GO:0005634 (nucleus), GO:0005856 (cytoskeleton), GO:0005819 (spindle), GO:0015630 (microtubule cytoskeleton)