Protein: Actin-like protein MamK (EC 3.6.1.-) (Magnetosome cytoskeleton protein MamK) (Q2W8Q6)
Organism: Paramagnetospirillum magneticum (strain ATCC 700264 / AMB-1) (Magnetospirillum magneticum)
Function: Protein with ATPase activity which forms dynamic cytoplasmic filaments (probably with paralog MamK-like) that organize magnetosomes into long chains running parallel to the long axis of the cell (Probable). Turnover of MamK filaments is probably promoted by MamK-like, which provides a monomer pool. Forms twisted filaments in the presence of ATP or GTP. Serves to close gaps between magnetosomes in the chain. Interaction with MCP10 is involved in controlling the response to magnetic fields, possibly by controlling flagellar rotation. Expression in E.coli yields a filament in the cell's longitudinal axis; the protein nucleates at several sites and one extremity of the filament is located at the cell pole.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:16373532, ECO:0000305|PubMed:20471399}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:16373532, ECO:0000269|PubMed:17085581, ECO:0000269|PubMed:20471399, ECO:0000269|PubMed:21883528, ECO:0000269|PubMed:24957623, ECO:0000269|PubMed:27481925}. Magnetosome membrane {ECO:0000305|PubMed:16303747}; Peripheral membrane protein {ECO:0000305}. Note=Tagged protein forms straight lines extending along most of the cell associated with its inner curvature, in the correct position to be filaments that are seen associated with magnetosomes (PubMed:16373532, PubMed:17085581, PubMed:20471399, PubMed:21883528, PubMed:27481925). Colocalizes in filaments with paralog MamK-like (PubMed:24957623). {ECO:0000269|PubMed:16373532, ECO:0000269|PubMed:17085581, ECO:0000269|PubMed:20471399, ECO:0000269|PubMed:21883528, ECO:0000269|PubMed:24957623, ECO:0000269|PubMed:27481925}.
Sequence: MSEGEGQAKNRLFLGIDLGTSHTAVMSSRGKKFLLKSVVGYPKDVIGLKLLGRPYVVGDEAFEMRSYLDIRYPLQDGVLSEISDRDIEVARHLLTHVVKSAEPGPNDEICAVIGVPARASAANKALLLKMAQEVVHTALVVSEPFMVGYGLDKLINTIIVDIGAGTTDICALKGTVPGPEDQVTLTKAGNYVDERLQNAILERHPELQMNVNVACAVKEQFSFVGTPTEVASFEFRAAGKPVRADVTEPVKIACEALMPDIIESIETLLRSFQPEYQATVLQNIVFAGGGSRIRGLAAYVKEKLRPFGDANVTCVKDPTFDGCRGALRLAEELPPQYWRQLGDVSGS
InterPro domains: - IPR043129: ATPase, nucleotide binding domain (homologous_superfamily) [14-331]
- IPR004000: Actin family (family) [11-342]
- IPR056546: MreB/MamK-like (family) [13-319]
Interaction partners: - Cell shape-determining protein MreC
- Magnetosome protein MamH
- Probable membrane transporter protein MamO
- Magnetosome formation protease MamE
- Magnetosome-associated protein MamJ
- Magnetosome protein MamM
- Magnetosome protein MamL
- Magnetosome protein MamI
- Magnetosome protein MamN
- Cytoskeleton protein RodZ-like C-terminal domain-containing protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0042802 (identical protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0016043 (cellular component organization), GO:0044085 (cellular component biogenesis), GO:0006996 (organelle organization), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton)