Protein: RNA replication protein (165 kDa protein) (ORF1 protein) [Includes: RNA-directed RNA polymerase (EC 2.7.7.48); Helicase (EC 3.6.4.13)] (P17779)
Organism: Potato virus X (strain X3) (PVX)
Function: RNA replication. The central part of this protein possibly functions as an ATP-binding helicase (Probable).
Sequence: MAKVREVYQSFTDSTTKTLIQDEAYRNIRPIMEKHKLANPYAQTVEAANDLEGFGIATNPYSIELHTHAAAKTIENKLLEVLGSILPQEPVTFMFLKPRKLNYMRRNPRIKDIFHNVAIEPRDVARYPKETIIDKLTEITTETAYISDTLHFLDPSYIVETFQNCPKLQTLYATLVLPVEAAFKMESTHPNIYSLKYFGDGFQYIPGNHGGGAYHHEFAHLQWLKVGKIKWRDPKDSFLGHLNYTTEQVEMHTVTVQLQESFAANHLYCIRRGDLLTPEVRTFGQPDRYVIPPQIFLPKVHNCKKPILKKTMMQLFLYVRTVKVAKNCDIFAKVRQLIKSSDLDKYSAVELVYLVSYMEFLADLQATTCFSDTLSGGLLTKTLAPVRAWIQEKKMQLFGLEDYAKLVKAVDFHPVDFSFKVETWDFRFHPLQAWKAFRPREVSDVEEMESLFSDGDLLDCFTRMPAYAVNAEEDLATIRKTPEMDVGQEVKEPAGDRNQYSNPAETFLNKLHRKHSREVKHQAVKKAKRLAEIQESMRAEGEAEPNETSGGMGAIPSNAELPGTSDARQELTLPTTKPVPARWEDASFTDSSVEEEQVRLLGEEAVKTATQQVIEGLPWKHWIPQLNAVGFKALLIQRDRSGTMIMPITEMVSGLEKEDFPEGTPKELARELLVMNRSPATIPLDLLRARDYGSDVKNKRIGAITKTQATSWGEYLTGKIESLTERKVATCVIHGAGGSGKSHAIQKALREIGKGSDITVVLPTNELRLDWSKKVPNTEPYMFKTYEKALIGGTGSIVIFDDYSKLPPGYIEALVCFYSKIKLIILTGDSRQSVYHETAEDASIRHLGPATEYFSKYCRYYLNATHRNKKDLANMLGVYSERTGVTEISMSAEFLEGIPTLVPSDEKRKLYMGTGRNDTFTYAGCQGLTKPKVQIVLDHNTQVCSANVMYTALSRATDRIHFVNTSANSSAFWEKLDSTPYLKTFLSVVREQALREYEPAEAEPIQEPEPQTHMCVENEESVLEEYKEELLEKFDREIHSESHGHSNCVQTEDTTIQLFSHQQAKDETLLWATIDARLKTSNQEANFREFLSKKDIGDVLFLNYQKAMGLPKERIPFSQEVWEACAHEVQSKYLSKSKCNLINGTVRQSPDFDENKIMVFLKSQWVTKVEKLGLPKIKPGQTIAAFYQQTVMLFGTMARYMRWFRQAFQPKEVFINCETTPEDMSVWALNNWNFSRPSLANDYTAFDQSQDGAMLQFEVLKAKHHCIPEEIIQAYIDIKTNAQIFLGTLSIMRLTGEGPTFDANTECNIAYTHTKFDIPAGTAQVYAGDDSALDCVPEVKHSFHRLEDKLLLKSKPVITQQKKGSWPEFCGWLITPKGVMKDPIKLHVSLKLAEAKGELKKCQDSYEIDLSYAYDHKDSLHDLFDEKQCQAHTLTCRTLIKSGRGTVSLPRLRNFL
InterPro domains: - IPR001788: RNA-dependent RNA polymerase, alsuviricetes (domain) [1127-1397]
- IPR043502: DNA/RNA polymerase superfamily (homologous_superfamily) [1079-1427]
- IPR002588: Alphavirus-like methyltransferase domain (domain) [39-339]
- IPR027351: (+) RNA virus helicase core domain (domain) [695-997]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [726-967]
- IPR007094: RNA-directed RNA polymerase, catalytic domain (domain) [1236-1343]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0016032 (viral process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044068 (modulation by symbiont of host cellular process), GO:0019058 (viral life cycle), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0019079 (viral genome replication), GO:0035821 (modulation of process of another organism), GO:0071704 (organic substance metabolic process), GO:0048523 (negative regulation of cellular process), GO:0019048 (modulation by virus of host process), GO:0044403 (biological process involved in symbiotic interaction), GO:0044238 (primary metabolic process), GO:0009892 (negative regulation of metabolic process), GO:0051701 (biological process involved in interaction with host), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0051129 (negative regulation of cellular component organization), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0039657 (suppression by virus of host gene expression), GO:0044003 (modulation by symbiont of host process), GO:0039694 (viral RNA genome replication), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0051128 (regulation of cellular component organization), GO:0043170 (macromolecule metabolic process), GO:0019054 (modulation by virus of host cellular process), GO:0044087 (regulation of cellular component biogenesis), GO:0052026 (modulation by symbiont of host transcription), GO:0009059 (macromolecule biosynthetic process), GO:0010639 (negative regulation of organelle organization), GO:1901362 (organic cyclic compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0033043 (regulation of organelle organization), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0019056 (modulation by virus of host transcription), GO:0039656 (modulation by virus of host gene expression), GO:1902115 (regulation of organelle assembly), GO:0010467 (gene expression), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0016070 (RNA metabolic process), GO:1902116 (negative regulation of organelle assembly), GO:0032774 (RNA biosynthetic process), GO:0006396 (RNA processing), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0036260 (RNA capping), GO:0006370 (7-methylguanosine mRNA capping), GO:0009452 (7-methylguanosine RNA capping)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0018995 (host cellular component), GO:0033643 (host cell part), GO:0043657 (host cell), GO:0033646 (host intracellular part), GO:0043656 (host intracellular region), GO:0030430 (host cell cytoplasm)