Protein: Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial (EC 1.14.11.-) (Alkylated DNA repair protein alkB homolog 7) (Q9D6Z0)
Organism: Mus musculus (Mouse)
Function: May function as protein hydroxylase; can catalyze auto-hydroxylation at Leu-110 (in vitro), but this activity may be due to the absence of the true substrate. Required to induce programmed necrosis in response to DNA damage caused by cytotoxic alkylating agents. Acts by triggering the collapse of mitochondrial membrane potential and loss of mitochondrial function that leads to energy depletion and cell death. ALKBH7-mediated necrosis is probably required to prevent the accumulation of cells with DNA damage. Does not display DNA demethylase activity (By similarity). Involved in fatty acid metabolism.
Subcellular location: Mitochondrion matrix {ECO:0000269|PubMed:23572141}.
Sequence: MAGSRRLAMRLLSGCAWVRGSDSAVLGRLRDEAVVHPGFLSQEEEDTLTRELEPQLRRRRYEYDHWDAAIHGFRETEKSCWSDASQVILQRVRAAAFGPDQSLLSPVHVLDLEPRGYIKPHVDSVKFCGSTIAGLSLLSPSVMKLVHTQEPEQWLELLLEPGSLYILRGSARYDFSHEILRDEESFFGEHRVPRGRRISVICRSLPEGMGPGRPEEPPPAC
InterPro domains: - IPR032870: Alpha-ketoglutarate-dependent dioxygenase alkB homologue 7-like (family) [12-209]
- IPR037151: Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily (homologous_superfamily) [16-215]
- IPR027450: Alpha-ketoglutarate-dependent dioxygenase AlkB-like (domain) [114-203]
Interaction partners: - Probable RNA/DNA demethylase ALKBH6
- Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4
- tRNA
- Nucleic acid dioxygenase ALKBH1
- DNA oxidative demethylase ALKBH2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0032451 (demethylase activity), GO:0016491 (oxidoreductase activity), GO:0051213 (dioxygenase activity), GO:0140097 (catalytic activity, acting on DNA), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0016706 (2-oxoglutarate-dependent dioxygenase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0050896 (response to stimulus), GO:0051234 (establishment of localization), GO:0051716 (cellular response to stimulus), GO:0065008 (regulation of biological quality), GO:0051641 (cellular localization), GO:0071840 (cellular component organization or biogenesis), GO:0006950 (response to stress), GO:0008219 (cell death), GO:0090559 (regulation of membrane permeability), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0012501 (programmed cell death), GO:0046907 (intracellular transport), GO:0033554 (cellular response to stress), GO:0070265 (necrotic cell death), GO:0006810 (transport), GO:0006839 (mitochondrial transport), GO:0046902 (regulation of mitochondrial membrane permeability), GO:0006996 (organelle organization), GO:0006974 (cellular response to DNA damage stimulus), GO:0097300 (programmed necrotic cell death), GO:0061024 (membrane organization), GO:0007005 (mitochondrion organization), GO:0007006 (mitochondrial membrane organization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)