Protein: Formate hydrogenlyase transcriptional activator FhlA (P19323)
Organism: Escherichia coli (strain K12)
Function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes. Also activates expression of hyf operon (encodes the silent hydrogenase-4 gene cluster).
Sequence: MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFCETWPQLDAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIVSVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAFTFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIVLPEPETPPAATVVALEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI
InterPro domains: - IPR029016: GAF-like domain superfamily (homologous_superfamily) [12-355]
- IPR003593: AAA+ ATPase domain (domain) [401-545]
- IPR003018: GAF domain (domain) [26-354]
- IPR025944: Sigma-54 interaction domain, conserved site (conserved_site) [594-603]
- IPR009057: Homedomain-like superfamily (homologous_superfamily) [593-687]
- IPR002078: RNA polymerase sigma factor 54 interaction domain (domain) [381-610]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [368-618]
- IPR002197: DNA binding HTH domain, Fis-type (domain) [603-687]
- IPR058031: NorR-like, AAA+ ATPase lid domain (domain) [553-620]
- IPR025662: Sigma-54 interaction domain, ATP-binding site 1 (binding_site) [405-418]
Interaction partners: - Formate dehydrogenase H
- Formate hydrogenlyase subunit 5
- Formate hydrogenlyase subunit 2
- Formate hydrogenlyase regulatory protein HycA
- Hydrogenase 3 maturation protease
- RNA polymerase sigma-54 factor
- Carbamoyl dehydratase HypE
- Hydrogenase-1 large chain
- Hydrogenase-2 large chain
- Formate channel FocA
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140299 (small molecule sensor activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0097159 (organic cyclic compound binding), GO:0043167 (ion binding), GO:0019826 (oxygen sensor activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0046906 (tetrapyrrole binding), GO:0019825 (oxygen binding), GO:0043169 (cation binding), GO:0020037 (heme binding), GO:0042578 (phosphoric ester hydrolase activity), GO:0046872 (metal ion binding), GO:0000287 (magnesium ion binding), GO:0008081 (phosphoric diester hydrolase activity), GO:0046914 (transition metal ion binding), GO:0005506 (iron ion binding), GO:0030145 (manganese ion binding), GO:0008198 (ferrous iron binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0031323 (regulation of cellular metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0010467 (gene expression), GO:0010468 (regulation of gene expression), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0016070 (RNA metabolic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006355 (regulation of DNA-templated transcription), GO:0006352 (DNA-templated transcription initiation), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0006351 (DNA-templated transcription), GO:2000142 (regulation of DNA-templated transcription initiation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)