Protein: Ferrochelatase, mitochondrial (EC 4.98.1.1) (Heme synthase) (Protoheme ferro-lyase) (Q9V9S8)
Organism: Drosophila melanogaster (Fruit fly)
Function: Catalyzes the ferrous insertion into protoporphyrin IX. Terminal enzyme in heme biosynthesis. Contains four conserved cysteines that function as cluster ligands and play a crucial role in maintaining protein structure.
Subcellular location: Mitochondrion inner membrane {ECO:0000250|UniProtKB:P22315}; Peripheral membrane protein {ECO:0000250|UniProtKB:P22315}; Matrix side {ECO:0000250|UniProtKB:P22315}.
Sequence: MFLHNTKFCRLASGLAGGVRNLSGQKPKTAILMLNMGGPTHTDQVHDYLLRIMTDRDMIQLPVQSRLGPWIAQRRTPEVQKKYKEIGGGSPILKWTELQGQLMCEQLDRISPETAPHKHYVGFRYVNPLTENTLAEIEKDKPERVVLFSQYPQYSCATSGSSFNSIFTHYRSNNLPSDIKWSIIDRWGTHPLLIKTFAQRIRDELAKFVETKRNDVVILFTAHSLPLKAVNRGDAYPSEIGASVHMVMQELGQTNPYSLAWQSKVGPLPWLAPATDDAIKGYVKQGLKNFILVPIAFVNEHIETLHELDIEYCDELAKEVGVEEIRRAATPNDHPLFIDALTNVVADHLKSQQAVNPKFLMRCPMCSNPKCRESKSWYRQLCSN
InterPro domains: - IPR001015: Ferrochelatase (family) [25-365]
- IPR033659: Ferrochelatase, N-terminal (domain) [29-190]
- IPR019772: Ferrochelatase, active site (active_site) [218-236]
- IPR033644: Ferrochelatase, C-terminal (domain) [195-332]
Interaction partners: - Protoporphyrinogen oxidase
- Uroporphyrinogen decarboxylase
- Frataxin homolog, mitochondrial
- Protoheme IX farnesyltransferase, mitochondrial
- Heme oxygenase
- Holocytochrome c-type synthase
- Oxygen-dependent coproporphyrinogen-III oxidase
- hydroxymethylbilane synthase
- Delta-aminolevulinic acid dehydratase
- 5-aminolevulinate synthase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016829 (lyase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0042440 (pigment metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0042168 (heme metabolic process), GO:0046483 (heterocycle metabolic process), GO:0046148 (pigment biosynthetic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0033013 (tetrapyrrole metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0006783 (heme biosynthetic process), GO:0006778 (porphyrin-containing compound metabolic process), GO:0033014 (tetrapyrrole biosynthetic process), GO:0006779 (porphyrin-containing compound biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)