Protein: Acyl-[acyl-carrier-protein] dehydrogenase MbtN (Acyl-ACP dehydrogenase MbtN) (EC 1.3.99.-) (Mycobactin synthase protein N) (P9WQF9)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Catalyzes the dehydrogenation at the alpha-beta position of ACP-bound acyl chains. This results in the introduction of a double bond in the lipidic chain, which is further transferred to the epsilon-amino group of lysine residue in the mycobactin core by MbtK.
Sequence: MTAGSDLDDFRGLLAKAFDERVVAWTAEAEAQERFPRQLIEHLGVCGVFDAKWATDARPDVGKLVELAFALGQLASAGIGVGVSLHDSAIAILRRFGKSDYLRDICDQAIRGAAVLCIGASEESGGSDLQIVETEIRSRDGGFEVRGVKKFVSLSPIADHIMVVARSVDHDPTSRHGNVAVVAVPAAQVSVQTPYRKVGAGPLDTAAVCIDTWVPADALVARAGTGLAAISWGLAHERMSIAGQIAASCQRAIGITLARMMSRRQFGQTLFEHQALRLRMADLQARVDLLRYALHGIAEQGRLELRTAAAVKVTAARLGEEVISECMHIFGGAGYLVDETTLGKWWRDMKLARVGGGTDEVLWELVAAGMTPDHDGYAAVVGASKA
InterPro domains: - IPR037069: Acyl-CoA dehydrogenase/oxidase, N-terminal domain superfamily (homologous_superfamily) [1-115]
- IPR013786: Acyl-CoA dehydrogenase/oxidase, N-terminal (domain) [9-100]
- IPR036250: Acyl-CoA dehydrogenase-like, C-terminal (homologous_superfamily) [224-371]
- IPR006091: Acyl-CoA oxidase/dehydrogenase, middle domain (domain) [117-211]
- IPR050741: Acyl-CoA dehydrogenase (family) [12-371]
- IPR009100: Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain superfamily (homologous_superfamily) [5-220]
- IPR046373: Acyl-CoA oxidase/dehydrogenase, middle domain superfamily (homologous_superfamily) [116-222]
- IPR009075: Acyl-CoA dehydrogenase/oxidase, C-terminal (domain) [224-370]
Interaction partners: - Medium/long-chain-fatty-acid--[acyl-carrier-protein] ligase MbtM
- Acyl carrier protein MbtL
- Probable fatty oxidation protein FadB
- Lysine N-acyltransferase MbtK
- Electron transfer flavoprotein subunit beta
- Electron transfer flavoprotein subunit alpha
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0016491 (oxidoreductase activity), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:1901265 (nucleoside phosphate binding), GO:0050660 (flavin adenine dinucleotide binding), GO:0071949 (FAD binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0006629 (lipid metabolic process), GO:0044282 (small molecule catabolic process), GO:0044248 (cellular catabolic process), GO:0006082 (organic acid metabolic process), GO:0044255 (cellular lipid metabolic process), GO:1901575 (organic substance catabolic process), GO:0006631 (fatty acid metabolic process), GO:0016054 (organic acid catabolic process), GO:0043436 (oxoacid metabolic process), GO:0030258 (lipid modification), GO:0044242 (cellular lipid catabolic process), GO:0016042 (lipid catabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0009062 (fatty acid catabolic process), GO:0019395 (fatty acid oxidation), GO:0046395 (carboxylic acid catabolic process), GO:0034440 (lipid oxidation), GO:0006635 (fatty acid beta-oxidation), GO:0032787 (monocarboxylic acid metabolic process), GO:0072329 (monocarboxylic acid catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)