Protein: Clathrin coat assembly protein AP180 (91 kDa synaptosomal-associated protein) (Clathrin coat-associated protein AP180) (Q05140)
Organism: Rattus norvegicus (Rat)
Function: Adaptins are components of the adapter complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats.
Subcellular location: Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane.
Sequence: MSGQTLTDRIAAAQYSVTGSAVARAVCKATTHEVMGPKKKHLDYLIQATNETNVNIPQMADTLFERATNSSWVVVFKALVTTHHLMVHGNERFIQYLASRNTLFNLSNFLDKSGSHGYDMSTFIRRYSRYLNEKAFSYRQMAFDFARVKKGADGVMRTMVPEKLLKSMPILQGQIDALLEFDVHPNELTNGVINAAFMLLFKDLIKLFACYNDGVINLLEKFFEMKKGQCKDALEIYKRFLTRMTRVSEFLKVADEVGIDKGDIPDLTQAPSSLMETLEQHLNTLEGKKPGNNEGSGAPSPLSKSSPATTVTSPNSTPAKTIDTSPPVDIFATASAAAPVSSAKPSSDLLDLQPDFSGARAGAAAPVPPPTGGATAWGDLLGEDSLAALSSVPSEAPISDPFAPEPSPPTTTTEPASASASATTAVTAATTEVDLFGDAFAASPGEAPAASEGATAPATPAPVAAALDACSGNDPFAPSEGSAEAAPELDLFAMKPPETSAPVVTPTASTAPPVPATAPSPAPTAVAATAATTTAAAAATTTATTSAAAATTAAAPPALDIFGDLFDSAPEVAAASKPDVAPSIDLFGTDAFSSPPRGASPVPESSLTADLLSGSGFHCAEDDRHVPLFFTAVDAFAAPSPASTASPAKAESSGVIDLFGDAFGSSASETQPAPQAVSSSSASADLLAGFGGSFMAPSTTPVTPAQNNLLQPNFEAAFGTTPSTSSSSSFDPSGDLLMPTMAPSGQPAPVSMVPPSPAMSASKGLGSDLDSSLASLVGNLGISGTTSKKGDLQWNAGEKKLTGGANWQPKVTPATWSAGVPPQGTVPPTSSVPPGAGAPSVGQPGAGYGMPPAGTGMTMMPQQPVMFAQPMMRPPFGAAAVPGTQLSPSPTPATQSPKKPPAKDPLADLNIKDFL
InterPro domains: - IPR008942: ENTH/VHS (homologous_superfamily) [1-148]
- IPR013809: ENTH domain (domain) [14-145]
- IPR011417: AP180 N-terminal homology (ANTH) domain (domain) [22-283]
- IPR014712: ANTH domain superfamily (homologous_superfamily) [147-295]
- IPR045192: Clathrin coat assembly protein AP180-like (family) [1-752]
Interaction partners: - Epsin-1
- Epsin-2
- Epidermal growth factor receptor pathway substrate 15
- Clathrin heavy chain 1
- AP-2 complex subunit alpha-2
- Vesicle-associated membrane protein 2
- AP2-associated protein kinase 1
- Endophilin-A1
- Intersectin-1
- Epsin-3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0008289 (lipid binding), GO:0043167 (ion binding), GO:0000149 (SNARE binding), GO:0005543 (phospholipid binding), GO:0043168 (anion binding), GO:0042802 (identical protein binding), GO:0019904 (protein domain specific binding), GO:0030276 (clathrin binding), GO:0017124 (SH3 domain binding), GO:0035091 (phosphatidylinositol binding), GO:0005546 (phosphatidylinositol-4,5-bisphosphate binding), GO:0005545 (1-phosphatidylinositol binding), GO:1901981 (phosphatidylinositol phosphate binding), GO:1902936 (phosphatidylinositol bisphosphate binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0007267 (cell-cell signaling), GO:0065008 (regulation of biological quality), GO:0051641 (cellular localization), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0140352 (export from cell), GO:0007154 (cell communication), GO:0051640 (organelle localization), GO:0099536 (synaptic signaling), GO:0044085 (cellular component biogenesis), GO:0051656 (establishment of organelle localization), GO:0006810 (transport), GO:0051649 (establishment of localization in cell), GO:0001505 (regulation of neurotransmitter levels), GO:0032940 (secretion by cell), GO:0016043 (cellular component organization), GO:0051648 (vesicle localization), GO:0023061 (signal release), GO:0006836 (neurotransmitter transport), GO:0036465 (synaptic vesicle recycling), GO:0099504 (synaptic vesicle cycle), GO:0140238 (presynaptic endocytosis), GO:0097479 (synaptic vesicle localization), GO:0099643 (signal release from synapse), GO:0099537 (trans-synaptic signaling), GO:0046903 (secretion), GO:0048489 (synaptic vesicle transport), GO:0007269 (neurotransmitter secretion), GO:0016192 (vesicle-mediated transport), GO:0043933 (protein-containing complex organization), GO:0006996 (organelle organization), GO:0051650 (establishment of vesicle localization), GO:0061024 (membrane organization), GO:0022607 (cellular component assembly), GO:0006900 (vesicle budding from membrane), GO:0098916 (anterograde trans-synaptic signaling), GO:0006897 (endocytosis), GO:0099003 (vesicle-mediated transport in synapse), GO:0065003 (protein-containing complex assembly), GO:0016050 (vesicle organization), GO:0048488 (synaptic vesicle endocytosis), GO:0048268 (clathrin coat assembly), GO:0007268 (chemical synaptic transmission)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0150034 (distal axon), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0098793 (presynapse), GO:0071944 (cell periphery), GO:0030054 (cell junction), GO:0044306 (neuron projection terminus), GO:0098833 (presynaptic endocytic zone), GO:0019898 (extrinsic component of membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0098835 (presynaptic endocytic zone membrane), GO:0019897 (extrinsic component of plasma membrane), GO:0120025 (plasma membrane bounded cell projection), GO:0043679 (axon terminus), GO:0031410 (cytoplasmic vesicle), GO:0031312 (extrinsic component of organelle membrane), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0045202 (synapse), GO:0030133 (transport vesicle), GO:0042734 (presynaptic membrane), GO:0098590 (plasma membrane region), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0099572 (postsynaptic specialization), GO:0097060 (synaptic membrane), GO:0030658 (transport vesicle membrane), GO:0030139 (endocytic vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0030118 (clathrin coat), GO:0098588 (bounding membrane of organelle), GO:0098888 (extrinsic component of presynaptic membrane), GO:0014069 (postsynaptic density), GO:0070382 (exocytic vesicle), GO:0043005 (neuron projection), GO:0060076 (excitatory synapse), GO:0030672 (synaptic vesicle membrane), GO:0098984 (neuron to neuron synapse), GO:0099243 (extrinsic component of synaptic membrane), GO:0031982 (vesicle), GO:0030659 (cytoplasmic vesicle membrane), GO:0032279 (asymmetric synapse), GO:0097708 (intracellular vesicle), GO:0099501 (exocytic vesicle membrane), GO:0030136 (clathrin-coated vesicle), GO:0030662 (coated vesicle membrane), GO:0045334 (clathrin-coated endocytic vesicle), GO:0030424 (axon), GO:0030669 (clathrin-coated endocytic vesicle membrane), GO:0030665 (clathrin-coated vesicle membrane)