Protein: Dual specificity protein phosphatase CDC14B (EC 3.1.3.16) (EC 3.1.3.48) (CDC14 cell division cycle 14 homolog B) (O60729)
Organism: Homo sapiens (Human)
Function: Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylates SIRT2 around early anaphase. Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.
Subcellular location: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Following DNA damage, translocates from the nucleolus to the nucleoplasm and interacts with FZR1/CDH1.
Sequence: MKRKSERRSSWAAAPPCSRRCSSTSPGVKKIRSSTQQDPRRRDPQDDVYLDITDRLCFAILYSRPKSASNVHYFSIDNELEYENFYADFGPLNLAMVYRYCCKINKKLKSITMLRKKIVHFTGSDQRKQANAAFLVGCYMVIYLGRTPEEAYRILIFGETSYIPFRDAAYGSCNFYITLLDCFHAVKKAMQYGFLNFNSFNLDEYEHYEKAENGDLNWIIPDRFIAFCGPHSRARLESGYHQHSPETYIQYFKNHNVTTIIRLNKRMYDAKRFTDAGFDHHDLFFADGSTPTDAIVKEFLDICENAEGAIAVHCKAGLGRTGTLIACYIMKHYRMTAAETIAWVRICRPGSVIGPQQQFLVMKQTNLWLEGDYFRQKLKGQENGQHRAAFSKLLSGVDDISINGVENQDQQEPEPYSDDDEINGVTQGDRLRALKSRRQSKTNAIPLTVILQSSVQSCKTSEPNISGSAGITKRTTRSASRKSSVKSLSISRTKTVLR
InterPro domains: - IPR029021: Protein-tyrosine phosphatase-like (homologous_superfamily) [39-379]
- IPR050561: Protein Tyrosine Phosphatase (family) [15-483]
- IPR000387: Tyrosine-specific protein phosphatases domain (domain) [297-359]
- IPR020422: Dual specificity protein phosphatase domain (domain) [214-374]
- IPR044506: Dual-specificity phosphatase CDC14, C-terminal (domain) [199-372]
- IPR003595: Protein-tyrosine phosphatase, catalytic (domain) [274-367]
- IPR016130: Protein-tyrosine phosphatase, active site (active_site) [312-322]
- IPR029260: Dual specificity/tyrosine protein phosphatase, N-terminal (domain) [52-190]
Interaction partners: - Dual specificity protein phosphatase CDC14A
- Fizzy-related protein homolog
- Mitogen-activated protein kinase 6
- Cell division cycle protein 27 homolog
- Anaphase-promoting complex subunit 1
- Cell division cycle protein 16 homolog
- Cell division cycle protein 23 homolog
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0004721 (phosphoprotein phosphatase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0042578 (phosphoric ester hydrolase activity), GO:0004722 (protein serine/threonine phosphatase activity), GO:0016791 (phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0044238 (primary metabolic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:1901564 (organonitrogen compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0019538 (protein metabolic process), GO:1901575 (organic substance catabolic process), GO:0043170 (macromolecule metabolic process), GO:0044248 (cellular catabolic process), GO:0009057 (macromolecule catabolic process), GO:0043933 (protein-containing complex organization), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0006996 (organelle organization), GO:0030163 (protein catabolic process), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0065003 (protein-containing complex assembly), GO:0042254 (ribosome biogenesis), GO:0022618 (ribonucleoprotein complex assembly), GO:0042273 (ribosomal large subunit biogenesis), GO:0071826 (ribonucleoprotein complex subunit organization), GO:0042255 (ribosome assembly), GO:0140694 (non-membrane-bounded organelle assembly), GO:0000027 (ribosomal large subunit assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0031981 (nuclear lumen), GO:0005819 (spindle), GO:0005730 (nucleolus), GO:0005694 (chromosome), GO:0005634 (nucleus), GO:0000793 (condensed chromosome), GO:0015630 (microtubule cytoskeleton)