Protein: NAD-dependent protein deacylase sirtuin-5, mitochondrial (EC 2.3.1.-) (Regulatory protein SIR2 homolog 5) (Q6DHI5)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo.
Subcellular location: Mitochondrion {ECO:0000255|HAMAP-Rule:MF_03160}. Cytoplasm, cytosol {ECO:0000255|HAMAP-Rule:MF_03160}. Nucleus {ECO:0000255|HAMAP-Rule:MF_03160}. Note=Mainly mitochondrial. Also present extramitochondrially, with a fraction present in the cytosol and very small amounts also detected in the nucleus. {ECO:0000255|HAMAP-Rule:MF_03160}.
Sequence: MIVRQLWCSRGSTSHLCAAVRLNWRSPKMTRPSSDLTAFREHFAKAKHIAIITGAGVSAESGVPTFRGPGGFWRKWQAQDLATPEAFSRDPSLVWEFYHYRREVMRSKMPNPAHLAIAECEARLGQQGRSVVIITQNIDELHHRAGSKHVYEIHGSLFKTRCMSCGEVKANHKSPICPALDGKGAPDPNTKEARIPVELLPRCERKSCNGLLRPHVVWFGETLDSDILTAVERELEKCDLCLVVGTSSIVYPAAMFAPQVASRGVPVAEFNMECTPATQRFKYHFEGPCGSTLPPALERHESEAV
InterPro domains: - IPR050134: NAD-dependent sirtuin protein deacylases (family) [33-301]
- IPR029035: DHS-like NAD/FAD-binding domain superfamily (homologous_superfamily) [34-294]
- IPR027546: Sirtuin, class III (family) [47-302]
- IPR003000: Sirtuin family (family) [54-252]
- IPR026591: Sirtuin, catalytic core small domain superfamily (homologous_superfamily) [64-222]
- IPR026590: Sirtuin family, catalytic core domain (domain) [29-303]
Interaction partners: - Glutamine-dependent NAD
- Nicotinamide phosphoribosyltransferase
- Nicotinamide phosphoribosyltransferase
- Nudix-type motif 12.
- Uncharacterized protein LOC393330
- NAD
- Nicotinamide nucleotide transhydrogenase 2.
- Uncharacterized protein.
- Ectonucleotide pyrophosphatase/phosphodiesterase 1
- Sirtuin 6
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0016810 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds), GO:0016811 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0062012 (regulation of small molecule metabolic process), GO:0010906 (regulation of glucose metabolic process), GO:0046890 (regulation of lipid biosynthetic process), GO:0010565 (regulation of cellular ketone metabolic process), GO:0019216 (regulation of lipid metabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0042304 (regulation of fatty acid biosynthetic process), GO:0019217 (regulation of fatty acid metabolic process), GO:0046320 (regulation of fatty acid oxidation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)