Protein: Alpha-barbatene synthase (AtBS) (EC 4.2.3.69) (Beta-chamigrene synthase) (EC 4.2.3.78) (Terpeneoid synthase 11) (AtTPS11) (Thujopsene synthase) (EC 4.2.3.79) (Q4KSH9)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Involved in the biosynthesis of over 15 sesquiterpenes (C15). The major products are (+)-alpha-barbatene (27.3%), (+)-thujopsene (17.8%) and (+)-beta-chamigrene (9.9%). Can use farnesyl diphosphate or geranyl diphosphate as substrates, but not geranylgeranyl diphosphate.
Subcellular location: Cytoplasm {ECO:0000305}.
Sequence: MEALGNFDYESYTNFTKLPSSQWGDQFLKFSIADSDFDVLEREIEVLKPKVRENIFVSSSTDKDAMKKTILSIHFLDSLGLSYHFEKEIEESLKHAFEKIEDLIADENKLHTISTIFRVFRTYGYYMSSDVFKIFKGDDGKFKESLIEDVKGMLSFYEAVHFGTTTDHILDEALSFTLNHLESLATGRRASPPHISKLIQNALHIPQHRNIQALVAREYISFYEHEEDHDETLLKLAKLNFKFLQLHYFQELKTITMWWTKLDHTSNLPPNFRERTVETWFAALMMYFEPQFSLGRIMSAKLYLVITFLDDACDTYGSISEVESLADCLERWDPDYMENLQGHMKTAFKFVMYLFKEYEEILRSQGRSFVLEKMIEEFKIIARKNLELVKWARGGHVPSFDEYIESGGAEIGTYATIACSIMGLGEIGKKEAFEWLISRPKLVRILGAKTRLMDDIADFEEDMEKGYTANALNYYMNEHGVTKEEASRELEKMNGDMNKIVNEECLKITTMPRRILMQSVNYARSLDVLYTADDVYNHREGKLKEYMRLLLVDPILL
InterPro domains: - IPR008930: Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid (homologous_superfamily) [23-228]
- IPR005630: Terpene synthase, metal-binding domain (domain) [261-499]
- IPR001906: Terpene synthase, N-terminal domain (domain) [22-203]
- IPR008949: Isoprenoid synthase domain superfamily (homologous_superfamily) [21-555]
- IPR034741: Terpene cyclase-like 1, C-terminal domain (domain) [230-555]
- IPR050148: Terpene synthase-like (family) [19-554]
- IPR044814: Terpene cyclases, class 1, plant (family) [15-554]
- IPR036965: Terpene synthase, N-terminal domain superfamily (homologous_superfamily) [64-229]
Interaction partners: - Inactive squalene synthase 2
- Squalene synthase 1
- Alpha-humulene/
- (Z)-gamma-bisabolene synthase 1 (Terpenoid synthase 12) (AtTPS12)
- (Z)-gamma-bisabolene synthase 2 (Terpenoid synthase 13) (AtTPS13)
- Farnesyl pyrophosphate synthase 2
- Farnesyl pyrophosphate synthase 1, mitochondrial
- B3 domain-containing protein At3g06220
- Heterodimeric geranylgeranyl pyrophosphate synthase large subunit 2
- Dehydrodolichyl diphosphate synthase 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0009975 (cyclase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0009058 (biosynthetic process), GO:0006950 (response to stress), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0009611 (response to wounding), GO:0019637 (organophosphate metabolic process), GO:0006793 (phosphorus metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0006720 (isoprenoid metabolic process), GO:0008299 (isoprenoid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0006644 (phospholipid metabolic process), GO:0045338 (farnesyl diphosphate metabolic process), GO:0006721 (terpenoid metabolic process), GO:0016114 (terpenoid biosynthetic process), GO:0006714 (sesquiterpenoid metabolic process), GO:0016106 (sesquiterpenoid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0042579 (microbody), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005777 (peroxisome)