Protein: L-carnitine dehydrogenase (CDH) (L-CDH) (EC 1.1.1.108) (Q9HTH8)
Organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Function: Catalyzes the NAD(+)-dependent oxidation of L-carnitine to 3-dehydrocarnitine. Is specific for L-carnitine and NAD(+) as substrates. D,L-3-hydroxybutyrate, L-lactate, ethanol, L-malate and D,L-isocitrate are not substrates. Is involved in a L-carnitine degradation pathway that allows P.aeruginosa to grow on L-carnitine as the sole source of carbon and nitrogen.
Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02129}.
Sequence: MSFVTEIKTFAALGSGVIGSGWIARALAHGLDVVAWDPAPGAEAALRARVANAWPALRKQGLAPGAAQERLRFVASIEECVGDADFIQESAPERLDLKLDLHARISAAARPDVLIGSSTSGLLPSEFYAEASHPERCLVGHPFNPVYLLPLVEVVGGERTAAEAVRAAMRVYESLGMRPLHVRKEVPGFIADRLLEALWREALHLVNDGVATTGEIDDAIRFGAGLRWSFMGTFLTYTLAGGNAGMRHFMAQFGPALQLPWTYLPAPELTEALIDRVVEGTAEQQGARSIAELERYRDDCLLAVLGAIRETKARHGFAFAE
InterPro domains: - IPR008927: 6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily (homologous_superfamily) [188-275]
- IPR036291: NAD(P)-binding domain superfamily (homologous_superfamily) [6-185]
- IPR006176: 3-hydroxyacyl-CoA dehydrogenase, NAD binding (domain) [11-185]
- IPR006108: 3-hydroxyacyl-CoA dehydrogenase, C-terminal (domain) [188-256]
- IPR022694: 3-hydroxyacyl-CoA dehydrogenase (family) [4-301]
- IPR026578: L-carnitine dehydrogenase (family) [8-318]
- IPR013328: 6-phosphogluconate dehydrogenase, domain 2 (homologous_superfamily) [189-310]
Interaction partners: - Betainyl-CoA thioesterase
- 3-dehydrocarnitine:acetyl-CoA trimethylamine transferase
- ABC-type glycine betaine transport system substrate-binding domain-containing protein
- Probable lipolytic enzyme
- Glutathione-independent formaldehyde dehydrogenase
- HTH-type transcriptional regulator CdhR
- 3-ketoacyl-CoA thiolase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0016491 (oxidoreductase activity), GO:0000166 (nucleotide binding), GO:0016614 (oxidoreductase activity, acting on CH-OH group of donors), GO:1901265 (nucleoside phosphate binding), GO:0016616 (oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor), GO:0017076 (purine nucleotide binding), GO:0030554 (adenyl nucleotide binding), GO:0051287 (NAD binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006575 (cellular modified amino acid metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0044248 (cellular catabolic process), GO:0006577 (amino-acid betaine metabolic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0042219 (cellular modified amino acid catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0006579 (amino-acid betaine catabolic process), GO:0009437 (carnitine metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)