Protein: Phosphoribosylamine--glycine ligase (EC 6.3.4.13) (GARS) (Glycinamide ribonucleotide synthetase) (Phosphoribosylglycinamide synthetase) (P15640)
Organism: Escherichia coli (strain K12)
Function: Catalyzes the reversible conversion of phosphoribosylamine to glycinamide ribonucleotide, an enzymatic step in purine biosynthesis pathway.
Sequence: MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQNVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLEGSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMTLEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMIDKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGVVMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGHTVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN
InterPro domains: - IPR016185: Pre-ATP-grasp domain superfamily (homologous_superfamily) [1-103]
- IPR020559: Phosphoribosylglycinamide synthetase, conserved site (conserved_site) [290-297]
- IPR020562: Phosphoribosylglycinamide synthetase, N-terminal (domain) [1-102]
- IPR011761: ATP-grasp fold (domain) [109-316]
- IPR000115: Phosphoribosylglycinamide synthetase (family) [1-428]
- IPR037123: Phosphoribosylglycinamide synthetase, C-domain superfamily (homologous_superfamily) [330-429]
- IPR011054: Rudiment single hybrid motif (homologous_superfamily) [329-424]
- IPR013815: ATP-grasp fold, subdomain 1 (homologous_superfamily) [121-190]
- IPR020561: Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain (domain) [103-296]
- IPR020560: Phosphoribosylglycinamide synthetase, C-domain (domain) [330-424]
Interaction partners: - Phosphoribosylformylglycinamidine synthase
- Amidophosphoribosyltransferase
- Bifunctional purine biosynthesis protein PurH
- N5-carboxyaminoimidazole ribonucleotide mutase
- Phosphoribosylglycinamide formyltransferase
- Phosphoribosylformylglycinamidine cyclo-ligase
- N5-carboxyaminoimidazole ribonucleotide synthase
- Formate-dependent phosphoribosylglycinamide formyltransferase
- Phosphoribosylaminoimidazole-succinocarboxamide synthase
- Adenylosuccinate lyase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0016740 (transferase activity), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0016879 (ligase activity, forming carbon-nitrogen bonds), GO:0016742 (hydroxymethyl-, formyl- and related transferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0009112 (nucleobase metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0006144 (purine nucleobase metabolic process), GO:0072522 (purine-containing compound biosynthetic process), GO:0046112 (nucleobase biosynthetic process), GO:0009113 (purine nucleobase biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)