Protein: DExH-box ATP-dependent RNA helicase DExH14 (EC 3.6.4.13) (BRR2 homolog C) (AtBRR2C) (Pre-mRNA-splicing helicase BRR2C) (Q9FNQ1)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly.
Subcellular location: Nucleus {ECO:0000305}.
Sequence: MVHNSPCNKHKTTSISWTRILAGRCTGLFVQVSNRRRFRTQAIAFFLGEPTRGENTTEEKNPKRHKYRDFVMLVQLPRLTSSLREPFDIDQAYLRRKTILQTLNKPRSSGNRLDESDLAKRIVHQWEGASLEVRQAYKQFIGAVVELIDREVPSDEFREVAFSAYRLFNNPVEEDDSDINDNISISGKKLELQNLVGHAVSDANVKNVASFAQALYSIQPTHQSETYADEVNGGAEFGADLVFNLPARFLVEASLDETGFVDVESNDAHTSFSEGWSGVSDTKNNLSAGKFNLSWLRDACGRMVRETNSQLSREELAMAICRFLDSDKPGEEIAGDLLDLVGDGAFETVQDLIMHRKEIVDAIHHGQMILKSDKAASNTQSRMPTYGTQVTVQTESAKQIEKLRRKEEKKNKRNADLGLESEISEANFSSLLEASEKKTAFEDLIGSGEANSLALALPQGTVRKHLKGYEEVFIPPTPTAQMKPGEKLIEIKELDDFAQAAFHGYKSLNRIQSRIFQTVYHTNENILVCAPTGAGKTNIAMISVLHEIKQHFRDGYLHKNEFKIVYVAPMKALAAEVTSAFSRRLAPLNMVVKELTGDMQLTKTELEETQMIVTTPEKWDVITRKSSDMSMSMLVKLLIIDEVHLLNDDRGAVIEALVARTLRQVESTQTMIRIVGLSATLPSYLQVAQFLRVNTDTGLFYFDSSYRPVPLAQQYIGITEHNFAARNELLNEICYKKVVDSIKQGHQAMIFVHSRKDTSKTAEKLVDLARQYETLDLFTNETHPQFQLMKKDVMKSRNKDLVKFFEAGFGIHHAGMLRSDRTLTERLFSDGLLKVLVCTATLAWGVNLPAHTVVIKGTQLYDAKAGGWKDLGMLDVMQIFGRAGRPQFDKSGEGIIITSHDKLAYYLRLLTSQLPIESQFISSLKDNLNAEVVLGTVTNVKEACAWLGYTYLSIRMKLNPLAYGIGWEEIIADPSLSLKQRALVADAARSLDKAKMMRFDEKSGNFYCTELGRVASHFYIQYSSVETYNEMLKRHMNESEIINMVAHSSEFENIVVREEEQHELETLARSCCPLEVKGGPSNKHGKISILIQLYISRGSIDAFSLVSDASYISASLARIMRALFEICLRKGWCEMTLFMLEYCKAVDRQLWPHQHPLRQFERDLPSDILRKLEERRDDLDHLYEMEEKEIGALIRYNPGGRLVKQHLGYFPSIQLAATVSPITRTVLKVDLLITPNFIWKDRFHGTALRWWILIEDTENDYIYHSDLFTLTKRMARGEPQKLSFTVPIFEPHPPQYYVHAVSDSWLHAETYFTISFHNLALPEARTSHTELLDLKPLPVTSLGNKLYESLYKFSHFNPIQTQIFHVLYHTDNNVLVGAPTGSGKTISAELAMLRLFSTQPDMKVVYIAPLKAIVRERMNDWKKHLVAPLGKEMVEMTGDYTPDLVALLSADIIISTPEKWDGISRNWHTRSYVKKVGLVILDEIHLLGADRGPILEVIVSRMRYISSQTERSVRFVGLSTALANAGDLADWLGVGEIGLFNFKPSVRPVPIEVHIQGYPGKYYCPRMNSMNKPAYAAICTHSPTKPVLIFVSSRRQTRLTALDLIQFAASDEHPRQFLSVSEEDLQMVLSQITDQNLRHTLQFGIGLHHAGLNDHDRSAVEELFTNNKIQVLVSTSTLAWGVNLPAHLVIIKGTEYFDGKTKRYVDFPLTEILQMMGRAGRPQFDQHGKAVILVHEPKKSFYKKFLYEPFPVESSLKEKLHDHFNAEIVSGTIGNKEDAVHYLTWTYLFRRLMANPAYYGLEGTQDETICSYLSRLVQTTFEDLEDSGCLKVNEDSVEPTMLGTIASQYYLCYMTVSMFGSNIGPDTSLEAFLHILAGASEYDELPVRHNEENYNKTLSDRVRYPVDNNHLDDPHVKANLLFQAHFSQLALPISDYNTDLKSVLDQSIRILQAMIDICANSGWLSSSLTCMRLLQMVMQGMWSDQDSSLWMIPCMNDLLLGSLTARGIHTLHQLLNLPRETLQSVTENFPASRLSQDLQRFPRIQMNVRLQKKDSDGKKKPSTLEIRLEKTSKRNSSRALAPRFPKVKDEAWWLVLGDTSTSELFAVKRVSFTGRLITRMELPPNITSFQDTKLILVSDCYLGFEQEHSIEQLARRG
InterPro domains: - IPR050474: Helicase Hel308/SKI2-like (family) [388-1226]
- IPR035892: C2 domain superfamily (homologous_superfamily) [1210-2153]
- IPR036390: Winged helix DNA-binding domain superfamily (homologous_superfamily) [915-1851]
- IPR056379: DExH14, plug domain (domain) [116-220]
- IPR003593: AAA+ ATPase domain (domain) [522-1545]
- IPR057842: SNRNP200 helicase-like, winged helix-turn-helix domain (domain) [917-1834]
- IPR004179: Sec63 domain (domain) [1008-2152]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [452-1752]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [504-1552]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [509-1523]
- IPR014756: Immunoglobulin E-set (homologous_superfamily) [2046-2150]
- IPR001650: Helicase, C-terminal domain-like (domain) [734-1780]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [917-1845]
Interaction partners: - Pre-mRNA-processing-splicing factor 8A
- Pre-mRNA-processing-splicing factor 8B
- Protein STABILIZED1
- ATP-dependent RNA helicase DEAH12, chloroplastic
- Transcription regulator/ zinc ion binding protein
- Cwf15 / Cwc15 cell cycle control family protein
- Pre-mRNA-splicing factor 18
- Small nuclear ribonucleoprotein Sm D2
- Nuclear protein-like
- Probable small nuclear ribonucleoprotein F
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0008380 (RNA splicing), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0000398 (mRNA splicing, via spliceosome), GO:0000375 (RNA splicing, via transesterification reactions), GO:0000380 (alternative mRNA splicing, via spliceosome), GO:0000377 (RNA splicing, via transesterification reactions with bulged adenosine as nucleophile)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0005886 (plasma membrane), GO:0009505 (plant-type cell wall)