Protein: Large ribosomal subunit protein uL2 (50S ribosomal protein L2) (P9WHA5)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.
Sequence: MAIRKYKPTTPGRRGASVSDFAEITRSTPEKSLVRPLHGRGGRNAHGRITTRHKGGGHKRAYRMIDFRRNDKDGVNAKVAHIEYDPNRTARIALLHYLDGEKRYIIAPNGLSQGDVVESGANADIKPGNNLPLRNIPAGTLIHAVELRPGGGAKLARSAGSSIQLLGKEASYASLRMPSGEIRRVDVRCRATVGEVGNAEQANINWGKAGRMRWKGKRPSVRGVVMNPVDHPHGGGEGKTSGGRHPVSPWGKPEGRTRNANKSSNKFIVRRRRTGKKHSR
InterPro domains: - IPR002171: Large ribosomal subunit protein uL2 (family) [4-275]
- IPR008991: Translation protein SH3-like domain superfamily (homologous_superfamily) [127-271]
- IPR022669: Large ribosomal subunit protein uL2, C-terminal (domain) [125-253]
- IPR014722: Large ribosomal subunit protein uL2, domain 2 (homologous_superfamily) [119-197]
- IPR005880: Large ribosomal subunit protein uL2, bacterial/organellar-type (family) [1-273]
- IPR022671: Large ribosomal subunit protein uL2, conserved site (conserved_site) [219-230]
- IPR014726: Large ribosomal subunit protein uL2, domain 3 (homologous_superfamily) [198-280]
- IPR022666: Large ribosomal subunit protein uL2, RNA-binding domain (domain) [42-119]
- IPR012340: Nucleic acid-binding, OB-fold (homologous_superfamily) [1-126]
Interaction partners: - Small ribosomal subunit protein uS15
- Large ribosomal subunit protein uL5
- Large ribosomal subunit protein bL36
- Small ribosomal subunit protein bS20
- Small ribosomal subunit protein uS17
- Large ribosomal subunit protein uL24
- Small ribosomal subunit protein uS5
- Large ribosomal subunit protein uL29
- Large ribosomal subunit protein uL14
- Large ribosomal subunit protein bL35
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0048522 (positive regulation of cellular process), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0048523 (negative regulation of cellular process), GO:0009892 (negative regulation of metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0051253 (negative regulation of RNA metabolic process), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0010468 (regulation of gene expression), GO:0042254 (ribosome biogenesis), GO:1902680 (positive regulation of RNA biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0045892 (negative regulation of DNA-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)