Protein: DNA polymerase IV 1 (Pol IV 1) (EC 2.7.7.7) (P9WNT3)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII (By similarity).
Subcellular location: Cytoplasm {ECO:0000250}.
Sequence: MESRWVLHLDMDAFFASVEQLTRPTLRGRPVLVGGLGGRGVVAGASYEARAYGARSAMPMHQARRLIGVTAVVLPPRGVVYGIASRRVFDTVRGLVPVVEQLSFDEAFAEPPQLAGAVAEDVETFCERLRRRVRDETGLIASVGAGSGKQIAKIASGLAKPDGIRVVRHAEEQALLSGLPVRRLWGIGPVAEEKLHRLGIETIGQLAALSDAEAANILGATIGPALHRLARGIDDRPVVERAEAKQISAESTFAVDLTTMEQLHEAIDSIAEHAHQRLLRDGRGARTITVKLKKSDMSTLTRSATMPYPTTDAGALFTVARRLLPDPLQIGPIRLLGVGFSGLSDIRQESLFADSDLTQETAAAHYVETPGAVVPAAHDATMWRVGDDVAHPELGHGWVQGAGHGVVTVRFETRGSGPGSARTFPVDTGDISNASPLDSLDWPDYIGQLSVEGSAGASAPTVDDVGDR
InterPro domains: - IPR017961: DNA polymerase, Y-family, little finger domain (domain) [245-349]
- IPR043502: DNA/RNA polymerase superfamily (homologous_superfamily) [3-241]
- IPR001126: UmuC domain (domain) [6-188]
- IPR043128: Reverse transcriptase/Diguanylate cyclase domain (homologous_superfamily) [6-168]
- IPR022880: DNA polymerase IV (family) [3-351]
- IPR036775: DNA polymerase, Y-family, little finger domain superfamily (homologous_superfamily) [241-350]
- IPR024728: DNA polymerase type-Y, HhH motif (conserved_site) [170-200]
- IPR050116: DNA polymerase type-Y (family) [3-362]
Interaction partners: - Protein RecA
- Beta sliding clamp
- LexA repressor
- Probable bifunctional transcriptional activator/DNA repair enzyme AlkA
- DNA polymerase I
- Putative lipoprotein LppK
- Error-prone DNA polymerase
- Probable DNA polymerase III
- UvrABC system protein B
- Uncharacterized protein Rv3395c
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0043167 (ion binding), GO:0005515 (protein binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0043169 (cation binding), GO:0046872 (metal ion binding), GO:0016301 (kinase activity), GO:0000287 (magnesium ion binding), GO:0046914 (transition metal ion binding), GO:0030145 (manganese ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009605 (response to external stimulus), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0007154 (cell communication), GO:0044238 (primary metabolic process), GO:0009991 (response to extracellular stimulus), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0044249 (cellular biosynthetic process), GO:0031668 (cellular response to extracellular stimulus), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0071496 (cellular response to external stimulus), GO:0043170 (macromolecule metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0009432 (SOS response), GO:0006259 (DNA metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0071897 (DNA biosynthetic process), GO:0006281 (DNA repair), GO:0000731 (DNA synthesis involved in DNA repair), GO:0006301 (postreplication repair), GO:0019985 (translesion synthesis), GO:0042276 (error-prone translesion synthesis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0071944 (cell periphery), GO:0005737 (cytoplasm), GO:0016020 (membrane), GO:0005886 (plasma membrane)