Protein: 1,4-alpha-glucan branching enzyme GlgB (EC 2.4.1.18) (1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase) (Alpha-(1->4)-glucan branching enzyme) (Glycogen branching enzyme) (BE) (P07762)
Organism: Escherichia coli (strain K12)
Function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.
Sequence: MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDATDVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFGPLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWDGRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETASLICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYAKWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVILDWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALYWIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQVSGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLTFGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLLFMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPYGFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTDSMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE
InterPro domains: - IPR044143: Glycogen branching enzyme GlgB, N-terminal Early set domain (domain) [118-221]
- IPR006048: Alpha-amylase/branching enzyme, C-terminal all beta (domain) [628-725]
- IPR013780: Glycosyl hydrolase, all-beta (homologous_superfamily) [623-728]
- IPR017853: Glycoside hydrolase superfamily (homologous_superfamily) [228-621]
- IPR006047: Glycosyl hydrolase family 13, catalytic domain (domain) [247-596]
- IPR037439: 1,4-alpha-glucan-branching enzyme (family) [6-728]
- IPR013783: Immunoglobulin-like fold (homologous_superfamily) [2-221]
- IPR004193: Glycoside hydrolase, family 13, N-terminal (domain) [125-207]
- IPR014756: Immunoglobulin E-set (homologous_superfamily) [16-223]
- IPR054169: 1,4-alpha-glucan branching enzyme GlgB, N-terminal domain (domain) [7-99]
- IPR006407: 1,4-alpha-glucan-branching enzyme, GlgB (family) [101-726]
Interaction partners: - Glycogen phosphorylase
- Glycogen synthase
- Glucose-1-phosphate adenylyltransferase
- Glycogen debranching enzyme
- 4-alpha-glucanotransferase
- Maltodextrin phosphorylase
- Phosphoglucomutase
- Cytoplasmic alpha-amylase
- Periplasmic alpha-amylase
- ATP synthase subunit beta
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016757 (glycosyltransferase activity), GO:0016758 (hexosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044249 (cellular biosynthetic process), GO:0005975 (carbohydrate metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0006091 (generation of precursor metabolites and energy), GO:0044262 (cellular carbohydrate metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0044264 (cellular polysaccharide metabolic process), GO:0016051 (carbohydrate biosynthetic process), GO:0005976 (polysaccharide metabolic process), GO:0034637 (cellular carbohydrate biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0015980 (energy derivation by oxidation of organic compounds), GO:0033692 (cellular polysaccharide biosynthetic process), GO:0044042 (glucan metabolic process), GO:0006112 (energy reserve metabolic process), GO:0000271 (polysaccharide biosynthetic process), GO:0006073 (cellular glucan metabolic process), GO:0009250 (glucan biosynthetic process), GO:0005977 (glycogen metabolic process), GO:0005978 (glycogen biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)