Protein: Kinesin-like protein KIF24 (Q5T7B8)
Organism: Homo sapiens (Human)
Function: Microtubule-dependent motor protein that acts as a negative regulator of ciliogenesis by mediating recruitment of CCP110 to mother centriole in cycling cells, leading to restrict nucleation of cilia at centrioles. Mediates depolymerization of microtubules of centriolar origin, possibly to suppress aberrant cilia formation. Following activation by NEK2 involved in disassembly of primary cilium during G2/M phase but does not disassemble fully formed ciliary axonemes. As cilium assembly and disassembly is proposed to coexist in a dynamic equilibrium may suppress nascent cilium assembly and, potentially, ciliar re-assembly in cells that have already disassembled their cilia ensuring the completion of cilium removal in the later stages of the cell cycle. Plays an important role in recruiting MPHOSPH9, a negative regulator of cilia formation to the distal end of mother centriole.
Subcellular location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000269|PubMed:21620453, ECO:0000269|PubMed:30375385}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:30375385}. Note=Primarily localizes to the mother centriole/basal body and is either absent at daughter centriole.
Sequence: MASWLYECLCEAELAQYYSHFTALGLQKIDELAKITMKDYSKLGVHDMNDRKRLFQLIKIIKIMQEEDKAVSIPERHLQTSSLRIKSQELRSGPRRQLNFDSPADNKDRNASNDGFEMCSLSDFSANEQKSTYLKVLEHMLPDDSQYHTKTGILNATAGDSYVQTEISTSLFSPNYLSAILGDCDIPIIQRISHVSGYNYGIPHSCIRQNTSEKQNPWTEMEKIRVCVRKRPLGMREVRRGEINIITVEDKETLLVHEKKEAVDLTQYILQHVFYFDEVFGEACTNQDVYMKTTHPLIQHIFNGGNATCFAYGQTGAGKTYTMIGTHENPGLYALAAKDIFRQLEVSQPRKHLFVWISFYEIYCGQLYDLLNRRKRLFAREDSKHMVQIVGLQELQVDSVELLLEVILKGSKERSTGATGVNADSSRSHAVIQIQIKDSAKRTFGRISFIDLAGSERAADARDSDRQTKMEGAEINQSLLALKECIRALDQEHTHTPFRQSKLTQVLKDSFIGNAKTCMIANISPSHVATEHTLNTLRYADRVKELKKGIKCCTSVTSRNRTSGNSSPKRIQSSPGALSEDKCSPKKVKLGFQQSLTVAAPGSTRGKVHPLTSHPPNIPFTSAPKVSGKRGGSRGSPSQEWVIHASPVKGTVRSGHVAKKKPEESAPLCSEKNRMGNKTVLGWESRASGPGEGLVRGKLSTKCKKVQTVQPVQKQLVSRVELSFGNAHHRAEYSQDSQRGTPARPASEAWTNIPPHQKEREEHLRFYHQQFQQPPLLQQKLKYQPLKRSLRQYRPPEGQLTNETPPLFHSYSENHDGAQVEELDDSDFSEDSFSHISSQRATKQRNTLENSEDSFFLHQTWGQGPEKQVAERQQSLFSSPRTGDKKDLTKSWVDSRDPINHRRAALDHSCSPSKGPVDWSRENSTSSGPSPRDSLAEKPYCSQVDFIYRQERGGGSSFDLRKDASQSEVSGENEGNLPSPEEDGFTISLSHVAVPGSPDQRDTVTTPLREVSADGPIQVTSTVKNGHAVPGEDPRGQLGTHAEYASGLMSPLTMSLLENPDNEGSPPSEQLVQDGATHSLVAESTGGPVVSHTVPSGDQEAALPVSSATRHLWLSSSPPDNKPGGDLPALSPSPIRQHPADKLPSREADLGEACQSRETVLFSHEHMGSEQYDADAEETGLDGSWGFPGKPFTTIHMGVPHSGPTLTPRTGSSDVADQLWAQERKHPTRLGWQEFGLSTDPIKLPCNSENVTWLKPRPISRCLARPSSPLVPSCSPKTAGTLRQPTLEQAQQVVIRAHQEQLDEMAELGFKEETLMSQLASNDFEDFVTQLDEIMVLKSKCIQSLRSQLQLYLTCHGPTAAPEGTVPS
InterPro domains: - IPR001660: Sterile alpha motif domain (domain) [1-64]
- IPR013761: Sterile alpha motif/pointed domain superfamily (homologous_superfamily) [2-73]
- IPR027640: Kinesin-like protein (family) [6-1352]
- IPR019821: Kinesin motor domain, conserved site (conserved_site) [445-456]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [213-547]
- IPR036961: Kinesin motor domain superfamily (homologous_superfamily) [203-550]
- IPR001752: Kinesin motor domain (domain) [221-552]
Interaction partners: - Centriolar coiled-coil protein of 110 kDa
- Centrosomal protein of 97 kDa
- Serine/threonine-protein kinase Nek2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003774 (cytoskeletal motor activity), GO:0140657 (ATP-dependent activity), GO:0003777 (microtubule motor activity), GO:0005515 (protein binding), GO:0008092 (cytoskeletal protein binding), GO:0015631 (tubulin binding), GO:0008017 (microtubule binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0022402 (cell cycle process), GO:0050794 (regulation of cellular process), GO:0007049 (cell cycle), GO:0071840 (cellular component organization or biogenesis), GO:0007017 (microtubule-based process), GO:0040012 (regulation of locomotion), GO:0007059 (chromosome segregation), GO:0044085 (cellular component biogenesis), GO:1903047 (mitotic cell cycle process), GO:2000145 (regulation of cell motility), GO:0007051 (spindle organization), GO:0016043 (cellular component organization), GO:0000226 (microtubule cytoskeleton organization), GO:0000278 (mitotic cell cycle), GO:0031109 (microtubule polymerization or depolymerization), GO:1902850 (microtubule cytoskeleton organization involved in mitosis), GO:0051225 (spindle assembly), GO:0098813 (nuclear chromosome segregation), GO:0140014 (mitotic nuclear division), GO:0007052 (mitotic spindle organization), GO:0043933 (protein-containing complex organization), GO:0030334 (regulation of cell migration), GO:0006996 (organelle organization), GO:0000070 (mitotic sister chromatid segregation), GO:0022411 (cellular component disassembly), GO:0097435 (supramolecular fiber organization), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0007010 (cytoskeleton organization), GO:0000819 (sister chromatid segregation), GO:0032984 (protein-containing complex disassembly), GO:0007019 (microtubule depolymerization), GO:0048285 (organelle fission), GO:0090307 (mitotic spindle assembly), GO:0051276 (chromosome organization), GO:0000280 (nuclear division), GO:0140694 (non-membrane-bounded organelle assembly), GO:0051261 (protein depolymerization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0045171 (intercellular bridge), GO:0031974 (membrane-enclosed lumen), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0031981 (nuclear lumen), GO:0005819 (spindle), GO:0005730 (nucleolus), GO:0005634 (nucleus), GO:0072686 (mitotic spindle), GO:0015630 (microtubule cytoskeleton)