Protein: Kinetochore protein NDC80 (80 kDa spindle component protein) (Nuclear division cycle protein 80) (Two-hybrid interaction with DMC1 protein 3) (P40460)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.
Subcellular location: Nucleus. Chromosome, centromere, kinetochore. Note=Associated with kinetochores.
Sequence: MQSSTSTDQHVLHHMDPHRFTSQIPTATSSQLRRRNSTNQGLTDMINKSIARNTISGTGIPTGGINKNKRTRSTVAGGTNGTALALNDKSNSRNSVSRLSINQLGSLQQHLSNRDPRPLRDKNFQSAIQEEIYDYLKKNKFDIETNHPISIKFLKQPTQKGFIIIFKWLYLRLDPGYGFTKSIENEIYQILKNLRYPFLESINKSQISAVGGSNWHKFLGMLHWMVRTNIKLDMCLNKVDRSLINQNTQEITILSQPLKTLDEQDQRQERYELMVEKLLIDYFTESYKSFLKLEDNYEPSMQELKLGFEKFVHIINTDIANLQTQNDNLYEKYQEVMKISQKIKTTREKWKALKSDSNKYENYVNAMKQKSQEWPGKLEKMKSECELKEEEIKALQSNISELHKILRKKGISTEQFELQNQEREKLTRELDKINIQSDKLTSSIKSRKLEAEGIFKSLLDTLRQYDSSIQNLTRSRSQLGHNVNDSSLKINISENLLDRDFHEGISYEQLFPKGSGINESIKKSILKLNDEIQERIKTIEKDNITLEKDIKNLKHDINEKTQINEKLELELSEANSKFELSKQENERLLVAQRIEIEKMEKKINDSNLLMKTKISDAEELVTSTELKLEELKVDLNRKRYKLHQQVIHVIDITSKFKINIQSSLENSENELGNVIEELRNLEFETEHNVTN
InterPro domains: - IPR040967: Domain of unknown function DUF5595 (domain) [269-334]
- IPR038273: Ndc80 domain superfamily (homologous_superfamily) [112-231]
- IPR055260: Kinetochore protein Ndc80, CH domain (domain) [106-229]
- IPR005550: Kinetochore protein Ndc80 (family) [1-689]
Interaction partners: - Kinetochore protein SPC24
- Kinetochore protein NUF2
- Kinetochore protein SPC25
- Protein STU2
- Aurora kinase
- Serine/threonine-protein kinase MPS1
- Spindle assembly checkpoint serine/threonine-protein kinase BUB1
- Structural maintenance of chromosomes protein 2
- Outer kinetochore KNL1 complex subunit SPC105
- Structural maintenance of chromosomes protein 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0060090 (molecular adaptor activity), GO:0030674 (protein-macromolecule adaptor activity), GO:0005515 (protein binding), GO:0042802 (identical protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0031503 (protein-containing complex localization), GO:0019222 (regulation of metabolic process), GO:0022402 (cell cycle process), GO:0032879 (regulation of localization), GO:0007049 (cell cycle), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0033036 (macromolecule localization), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0065009 (regulation of molecular function), GO:0007017 (microtubule-based process), GO:0051640 (organelle localization), GO:0048522 (positive regulation of cellular process), GO:0050790 (regulation of catalytic activity), GO:0044093 (positive regulation of molecular function), GO:0044085 (cellular component biogenesis), GO:1903047 (mitotic cell cycle process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0051305 (chromosome movement towards spindle pole), GO:0007051 (spindle organization), GO:0060341 (regulation of cellular localization), GO:0060255 (regulation of macromolecule metabolic process), GO:0051649 (establishment of localization in cell), GO:0000226 (microtubule cytoskeleton organization), GO:0008608 (attachment of spindle microtubules to kinetochore), GO:0009891 (positive regulation of biosynthetic process), GO:0045184 (establishment of protein localization), GO:0007018 (microtubule-based movement), GO:0007059 (chromosome segregation), GO:0046907 (intracellular transport), GO:0031325 (positive regulation of cellular metabolic process), GO:0051656 (establishment of organelle localization), GO:0010646 (regulation of cell communication), GO:0050000 (chromosome localization), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0070727 (cellular macromolecule localization), GO:0031323 (regulation of cellular metabolic process), GO:0016043 (cellular component organization), GO:0080090 (regulation of primary metabolic process), GO:0000278 (mitotic cell cycle), GO:1902850 (microtubule cytoskeleton organization involved in mitosis), GO:0098813 (nuclear chromosome segregation), GO:0044804 (autophagy of nucleus), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0140014 (mitotic nuclear division), GO:0000022 (mitotic spindle elongation), GO:0032880 (regulation of protein localization), GO:0010468 (regulation of gene expression), GO:0022607 (cellular component assembly), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0051225 (spindle assembly), GO:0006886 (intracellular protein transport), GO:0015031 (protein transport), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0007064 (mitotic sister chromatid cohesion), GO:0008104 (protein localization), GO:0051252 (regulation of RNA metabolic process), GO:0006996 (organelle organization), GO:0000070 (mitotic sister chromatid segregation), GO:0031328 (positive regulation of cellular biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0048285 (organelle fission), GO:0006355 (regulation of DNA-templated transcription), GO:0051276 (chromosome organization), GO:0007010 (cytoskeleton organization), GO:0051310 (metaphase plate congression), GO:0000819 (sister chromatid segregation), GO:1902680 (positive regulation of RNA biosynthetic process), GO:0090307 (mitotic spindle assembly), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045893 (positive regulation of DNA-templated transcription), GO:0000280 (nuclear division), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:0140694 (non-membrane-bounded organelle assembly), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0045944 (positive regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098687 (chromosomal region), GO:0099080 (supramolecular complex), GO:0000940 (outer kinetochore), GO:0043229 (intracellular organelle), GO:0000775 (chromosome, centromeric region), GO:0000776 (kinetochore), GO:0043228 (non-membrane-bounded organelle), GO:0000779 (condensed chromosome, centromeric region), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome), GO:0000793 (condensed chromosome)