Protein: Early boundary activity protein 1 (Blastoderm-specific gene 25A) (Q9VR17)
Organism: Drosophila melanogaster (Fruit fly)
Function: The heterotrimeric Elba complex is required for chromatin domain boundary function during early embryogenesis. It binds to a 8-bp sequence 5'-CCAATAAG-3' in the Fab-7 insulator or boundary element in the bithorax complex and contributes to its insulator or boundary activity. Elba1 may act as a transcriptional repressor and binds the palindromic sequence 5'-CCAATTGG-3' to mediate transcriptional repression.
Subcellular location: Nucleus {ECO:0000269|PubMed:23240086, ECO:0000269|PubMed:25561495}.
Sequence: MINRRQRLEFALTLLPYDPETVQLSETQKKVEAIIARLRTDDSFTEEESDDCKVRRIQDANEFADSAMRHIEMSDSGKLSTLETLTLAAEKLLRTQRSPDQDFDDMVQDVEYSQLMRNTIQAVNEARLKLLQQWERSKRKALDLLTIEIEKVQEMDQEPEHKQSHEQDQDQEQSSEPFNAFRDGADEHNTSTPKTNDEDLGLDDDDEDYVPGGEETMGNKRKRIKKPVTSTPNAKRRCPGFEFDLDGESPMVTIGPNGTEVSRISLSAINWDMTGPSITRKLLCEIFDRDTLAHHTLSGKPSPAFRDCARPSKQQLDPLKVADLVYLMTNSLDMTPREVRTAITTKCADENKMLRSRMQRKSK
InterPro domains: - IPR018379: BEN domain (domain) [255-354]
- IPR037496: BEN domain-containing protein 6-like (family) [277-359]
Interaction partners: - GEO07864p1
- Early boundary activity protein 3
- Halo
- Early boundary activity protein 2
- FI01028p
- RE73577p
- RE73396p
- Serendipity locus protein alpha
- Protein brother
- Uncharacterized protein, isoform A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003682 (chromatin binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0031490 (chromatin DNA binding), GO:0003677 (DNA binding), GO:0043035 (chromatin insulator sequence binding), GO:0043565 (sequence-specific DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0019953 (sexual reproduction), GO:0048869 (cellular developmental process), GO:0051641 (cellular localization), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0019222 (regulation of metabolic process), GO:0007389 (pattern specification process), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0032504 (multicellular organism reproduction), GO:0003006 (developmental process involved in reproduction), GO:0009892 (negative regulation of metabolic process), GO:0022412 (cellular process involved in reproduction in multicellular organism), GO:0050794 (regulation of cellular process), GO:0048523 (negative regulation of cellular process), GO:0048609 (multicellular organismal reproductive process), GO:0007276 (gamete generation), GO:0030707 (follicle cell of egg chamber development), GO:0007281 (germ cell development), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0009798 (axis specification), GO:0044085 (cellular component biogenesis), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0060255 (regulation of macromolecule metabolic process), GO:0051649 (establishment of localization in cell), GO:0031324 (negative regulation of cellular metabolic process), GO:0009880 (embryonic pattern specification), GO:0046907 (intracellular transport), GO:0009888 (tissue development), GO:0003002 (regionalization), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0006810 (transport), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0008069 (dorsal/ventral axis specification, ovarian follicular epithelium), GO:0016043 (cellular component organization), GO:0009790 (embryo development), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0035282 (segmentation), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0048477 (oogenesis), GO:0007028 (cytoplasm organization), GO:0007350 (blastoderm segmentation), GO:0010468 (regulation of gene expression), GO:0009948 (anterior/posterior axis specification), GO:0030855 (epithelial cell differentiation), GO:0022607 (cellular component assembly), GO:0007292 (female gamete generation), GO:0002064 (epithelial cell development), GO:0031326 (regulation of cellular biosynthetic process), GO:0000578 (embryonic axis specification), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0060429 (epithelium development), GO:0051253 (negative regulation of RNA metabolic process), GO:0007351 (tripartite regional subdivision), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0009952 (anterior/posterior pattern specification), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0008595 (anterior/posterior axis specification, embryo), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045892 (negative regulation of DNA-templated transcription), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0000122 (negative regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098687 (chromosomal region), GO:0031974 (membrane-enclosed lumen), GO:0000792 (heterochromatin), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000775 (chromosome, centromeric region), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005721 (pericentric heterochromatin), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005694 (chromosome), GO:0005634 (nucleus), GO:0005700 (polytene chromosome)