Protein: S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase, chloroplastic (Urophorphyrin III methylase) (EC 2.1.1.107) (Urophorphyrin methylase 1) (AtUPM1) (Q42606)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Essential protein required for siroheme biosynthesis. Catalyzes the two successive C-2 and C-7 methylation reactions involved in the conversion of uroporphyrinogen III to precorrin-2 via the intermediate formation of precorrin-1. It is a step in the biosynthesis of siroheme. Promotes nitrogen and sulfur assimilation as well as photosynthesis efficiency by triggering chlorophyll, nitrite reductase (NiR) and sulfite reductase (SiR) biosynthesis.
Subcellular location: Plastid, chloroplast {ECO:0000269|PubMed:9006913}.
Sequence: MALVQRIPISSSSIRNWQQARTNLTPICCLHYNTASSSSSPFTEKHSVERYQRDQWLYKAVEPTPPSTPSPSPFEDEVFVRENDIASQLPELKKLLAVLKEKRVKGCKGGDCGPGDVYLVGTGPGDPELLTLKAVRVIQSADLLLYDRLVSNDVLELVAPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIRVQVIPGITAASGIAAELGIPLTHRGVATSVRFLTGHSRKGGTDPLFVAENAADPDTTLVVYMGLGTLPSLAQKLMDHGLPSDTPAVAVERGTTPLQRTVFAELKDFATEIQSAGLVSPTLIIIGKVVELSPLWPHCTKESSCLVETR
InterPro domains: - IPR035996: Tetrapyrrole methylase superfamily (homologous_superfamily) [114-351]
- IPR000878: Tetrapyrrole methylase (domain) [117-328]
- IPR014776: Tetrapyrrole methylase, subdomain 2 (homologous_superfamily) [231-365]
- IPR014777: Tetrapyrrole methylase, subdomain 1 (homologous_superfamily) [109-230]
- IPR006366: Uroporphyrin-III C-methyltransferase (domain) [115-351]
- IPR050161: Siroheme/cobalamin biosynthesis enzyme (family) [37-367]
- IPR003043: Uroporphiryn-III C-methyltransferase, conserved site (conserved_site) [120-228]
- IPR012383: Uroporphyrin-III C-methyltransferase, plant (family) [1-369]
Interaction partners: - Porphobilinogen deaminase, chloroplastic
- Ferredoxin--nitrite reductase, chloroplastic
- Sirohydrochlorin ferrochelatase, chloroplastic
- Uroporphyrinogen decarboxylase 1, chloroplastic
- Uroporphyrinogen decarboxylase 2, chloroplastic
- Assimilatory sulfite reductase
- Uroporphyrinogen-III synthase, chloroplastic
- 5'-adenylylsulfate reductase 1, chloroplastic
- Rieske
- 5'-adenylylsulfate reductase 3, chloroplastic
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0008168 (methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0042440 (pigment metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0046148 (pigment biosynthetic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0015995 (chlorophyll biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0033013 (tetrapyrrole metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0006778 (porphyrin-containing compound metabolic process), GO:0033014 (tetrapyrrole biosynthetic process), GO:0006779 (porphyrin-containing compound biosynthetic process), GO:0015994 (chlorophyll metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle)