Protein: 5'(3')-deoxyribonucleotidase, cytosolic type (EC 3.1.3.-) (Cytosolic 5',3'-pyrimidine nucleotidase) (Deoxy-5'-nucleotidase 1) (dNT-1) (Q9JM14)
Organism: Mus musculus (Mouse)
Function: Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides, with a preference for dUMP and dTMP, intermediate activity towards dGMP, and low activity towards dCMP and dAMP.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:10681516}.
Sequence: MAVKRPVRVLVDMDGVLADFESGLLQGFRRRFPEEPHVPLEQRRGFLANEQYGALRPDLAEKVASVYESPGFFLNLEPIPGALDALREMNDMKDTEVFICTTPLLKYDHCVGEKYRWVEQNLGPEFVERIILTRDKTVVMGDLLIDDKDNIQGLEETPSWEHILFTCCHNQHLALPPTRRRLLSWSDNWRGIIESKRASL
InterPro domains: - IPR010708: 5'(3')-deoxyribonucleotidase (family) [5-193]
- IPR023214: HAD superfamily (homologous_superfamily) [9-189]
- IPR036412: HAD-like superfamily (homologous_superfamily) [8-196]
Interaction partners: - Cytosolic 5'-nucleotidase 1A
- Cytosolic 5'-nucleotidase 3A
- Cytosolic purine 5'-nucleotidase
- 7-methylguanosine phosphate-specific 5'-nucleotidase
- Cytosolic 5'-nucleotidase 1B
- Deoxyuridine triphosphatase.
- Thymidylate kinase
- Thymidine kinase 2, mitochondrial
- Deoxycytidine kinase
- Thymidine kinase, cytosolic
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0042578 (phosphoric ester hydrolase activity), GO:0016791 (phosphatase activity), GO:0008252 (nucleotidase activity), GO:0008253 (5'-nucleotidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0044248 (cellular catabolic process), GO:0019637 (organophosphate metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0006793 (phosphorus metabolic process), GO:0034655 (nucleobase-containing compound catabolic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0006753 (nucleoside phosphate metabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:1901136 (carbohydrate derivative catabolic process), GO:0019439 (aromatic compound catabolic process), GO:0019692 (deoxyribose phosphate metabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0046700 (heterocycle catabolic process), GO:0009262 (deoxyribonucleotide metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0046434 (organophosphate catabolic process), GO:0072527 (pyrimidine-containing compound metabolic process), GO:0009123 (nucleoside monophosphate metabolic process), GO:1901292 (nucleoside phosphate catabolic process), GO:0046386 (deoxyribose phosphate catabolic process), GO:0006220 (pyrimidine nucleotide metabolic process), GO:0009117 (nucleotide metabolic process), GO:0009394 (2'-deoxyribonucleotide metabolic process), GO:0009264 (deoxyribonucleotide catabolic process), GO:0072529 (pyrimidine-containing compound catabolic process), GO:0009162 (deoxyribonucleoside monophosphate metabolic process), GO:0009125 (nucleoside monophosphate catabolic process), GO:0006244 (pyrimidine nucleotide catabolic process), GO:0009129 (pyrimidine nucleoside monophosphate metabolic process), GO:0009219 (pyrimidine deoxyribonucleotide metabolic process), GO:0009166 (nucleotide catabolic process), GO:0009223 (pyrimidine deoxyribonucleotide catabolic process), GO:0009176 (pyrimidine deoxyribonucleoside monophosphate metabolic process), GO:0009159 (deoxyribonucleoside monophosphate catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)