Protein: Twinfilin-1 (Protein A6) (Protein tyrosine kinase 9) (Q12792)
Organism: Homo sapiens (Human)
Function: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity).
Subcellular location: Cytoplasm. Cytoplasm, cytoskeleton {ECO:0000250}. Note=Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts (By similarity). {ECO:0000250}.
Sequence: MSHQTGIQASEDVKEIFARARNGKYRLLKISIENEQLVIGSYSQPSDSWDKDYDSFVLPLLEDKQPCYILFRLDSQNAQGYEWIFIAWSPDHSHVRQKMLYAATRATLKKEFGGGHIKDEVFGTVKEDVSLHGYKKYLLSQSSPAPLTAAEEELRQIKINEVQTDVGVDTKHQTLQGVAFPISREAFQALEKLNNRQLNYVQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEGDYLESIVFIYSMPGYTCSIRERMLYSSCKSRLLEIVERQLQMDVIRKIEIDNGDELTADFLYEEVHPKQHAHKQSFAKPKGPAGKRGIRRLIRGPAETEATTD
InterPro domains: - IPR029006: ADF-H/Gelsolin-like domain superfamily (homologous_superfamily) [1-322]
- IPR002108: Actin-depolymerising factor homology domain (domain) [1-313]
- IPR028458: Twinfilin (family) [1-340]
Interaction partners: - F-actin-capping protein subunit beta
- F-actin-capping protein subunit alpha-1
- Actin, alpha skeletal muscle
- Cofilin-1
- Adenylyl cyclase-associated protein 1
- WD repeat-containing protein 82
- Gelsolin
- Unconventional myosin-Ic
- Destrin
- Plastin-1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0050839 (cell adhesion molecule binding), GO:0032553 (ribonucleotide binding), GO:0019899 (enzyme binding), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0045296 (cadherin binding), GO:0019900 (kinase binding), GO:0003723 (RNA binding), GO:0032555 (purine ribonucleotide binding), GO:0017076 (purine nucleotide binding), GO:0005524 (ATP binding), GO:0019901 (protein kinase binding), GO:0032559 (adenyl ribonucleotide binding), GO:0030554 (adenyl nucleotide binding), GO:0005080 (protein kinase C binding)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0042221 (response to chemical), GO:0051240 (positive regulation of multicellular organismal process), GO:0071840 (cellular component organization or biogenesis), GO:0051239 (regulation of multicellular organismal process), GO:0045927 (positive regulation of growth), GO:0065008 (regulation of biological quality), GO:0050793 (regulation of developmental process), GO:0009719 (response to endogenous stimulus), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0051094 (positive regulation of developmental process), GO:0040008 (regulation of growth), GO:0048522 (positive regulation of cellular process), GO:0048639 (positive regulation of developmental growth), GO:0045597 (positive regulation of cell differentiation), GO:0001558 (regulation of cell growth), GO:0070848 (response to growth factor), GO:0071495 (cellular response to endogenous stimulus), GO:1901700 (response to oxygen-containing compound), GO:0051130 (positive regulation of cellular component organization), GO:0030307 (positive regulation of cell growth), GO:0032970 (regulation of actin filament-based process), GO:0044089 (positive regulation of cellular component biogenesis), GO:0010033 (response to organic substance), GO:0044087 (regulation of cellular component biogenesis), GO:2000026 (regulation of multicellular organismal development), GO:0070887 (cellular response to chemical stimulus), GO:0022603 (regulation of anatomical structure morphogenesis), GO:0045595 (regulation of cell differentiation), GO:0016043 (cellular component organization), GO:0051128 (regulation of cellular component organization), GO:0051962 (positive regulation of nervous system development), GO:0090066 (regulation of anatomical structure size), GO:0048638 (regulation of developmental growth), GO:0032535 (regulation of cellular component size), GO:0045773 (positive regulation of axon extension), GO:0031344 (regulation of cell projection organization), GO:0071310 (cellular response to organic substance), GO:0060284 (regulation of cell development), GO:0010720 (positive regulation of cell development), GO:0032956 (regulation of actin cytoskeleton organization), GO:0050769 (positive regulation of neurogenesis), GO:0051960 (regulation of nervous system development), GO:0060491 (regulation of cell projection assembly), GO:0061387 (regulation of extent of cell growth), GO:0050770 (regulation of axonogenesis), GO:0031346 (positive regulation of cell projection organization), GO:0033993 (response to lipid), GO:0030516 (regulation of axon extension), GO:0120034 (positive regulation of plasma membrane bounded cell projection assembly), GO:0033043 (regulation of organelle organization), GO:1901701 (cellular response to oxygen-containing compound), GO:0071363 (cellular response to growth factor stimulus), GO:0032526 (response to retinoic acid), GO:0120032 (regulation of plasma membrane bounded cell projection assembly), GO:0051493 (regulation of cytoskeleton organization), GO:0120035 (regulation of plasma membrane bounded cell projection organization), GO:0071300 (cellular response to retinoic acid), GO:0050772 (positive regulation of axonogenesis), GO:0010976 (positive regulation of neuron projection development), GO:0008361 (regulation of cell size), GO:0071396 (cellular response to lipid), GO:1902745 (positive regulation of lamellipodium organization), GO:0050767 (regulation of neurogenesis), GO:0010592 (positive regulation of lamellipodium assembly), GO:1902743 (regulation of lamellipodium organization), GO:0010591 (regulation of lamellipodium assembly), GO:0010975 (regulation of neuron projection development)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0150034 (distal axon), GO:0042995 (cell projection), GO:0005615 (extracellular space), GO:0030427 (site of polarized growth), GO:0005737 (cytoplasm), GO:0005576 (extracellular region), GO:0120025 (plasma membrane bounded cell projection), GO:0030426 (growth cone), GO:0070062 (extracellular exosome), GO:0043227 (membrane-bounded organelle), GO:0043230 (extracellular organelle), GO:0065010 (extracellular membrane-bounded organelle), GO:0043005 (neuron projection), GO:0031982 (vesicle), GO:1903561 (extracellular vesicle), GO:0030424 (axon)