Protein: Spalt-like protein sem-4 (Sex muscle abnormal protein sem-4) (Transcription factor sem-4) (G5EFF4)
Organism: Caenorhabditis elegans
Function: Transcription factor, involved in positive and negative modulation of transcription. Binds to multiple DNA sequence motifs in the regulatory elements of target genes, including homeobox selector egl-5 and LIM homeobox mec-3. Involved in cell-fate regulation in multiple lineages, including neuronal, mesodermal and vulval. Required to regulate the fate of PLM touch receptor neurons, acting via negative modulation of transcription of egl-5 and mec-3. May modulate gene expression by interacting with different transcription factors during neuronal and mesodermal cell development. Promotes the proliferative sex myoblast (SM) fate, in a cell autonomous manner, acting via the SoxC transcription factor sem-2. Involved in vulval cell-fate determination, acting by regulating expression of homeobox protein lin-39, and may link lin-39 to incoming signaling pathways. Plays a role in detoxification of reactive oxygen species (ROS), by regulating expression of transcription factor skn-1 and the phase II detoxification genes.
Subcellular location: Nucleus {ECO:0000305}.
Sequence: MNELLAEMAAVSSRRKQSKPRRMSGEGDAMMSPIDLSTKSFDENNCEKGAGGALPLEDRSNILPHFSVPFANPQQFLSLCAQLGNSSSRNVSSTASTTSSCPIQSCSQSFSSPAALTWHVLDAHEDEQEIFSCDVCTTTFSNGQDIREHKCQKTLASRSTSVPPSTIPSSVCFLSTPTTPCLQFSINESIGTSEIREEDEEEDMDVEDGEHVANQLFGHLLQKSDDKSKMASLFNHAFPPFAAFPNMPPPFLMRQPFDPRADVFAAGRHDNDDDWEALMEISTSDEAEKIRALVGDKAVPTTDPNQCILCRRVLSCKSALQMHYRTHTGERPFKCKICQRAFTTKGNLKTHMGVHRSKHSFRGLPISLPPQLAAMHQHQHQIAPPQRIHIHNPPTSAASAAAAVAQIQASQQCPICQQRFLNAGELAVHITEHRNSLTQPPRVMPTPTTTRVQTFPFVPFFTTPPSLNATDMSTQFNLANILSAQLKNDSSPNTDTSSVEEKITRDDPPKMASLSPSNSSDSSSSVRQDILESSEFEEKLKKLEEPPILEQQVSTTPNPKNENPLLAMQKMWAETEPPPPRQMPVLSKHQCGVCFKHFSSSSALQIHMRTHTGDKPFKCDMCGRAFTTRGNLKVHMGTHSWQQSPSRRGRRIFDVASSVTEKPMLQSPILPTSGAPGASPLAMLGPNGLSGLEMMMMLWRTVCSVCQKVCQSPNELEQHLKEHLNNGSSAAPTPLASAATPPPS
InterPro domains: - IPR051565: Sal C2H2-type zinc-finger (family) [88-741]
- IPR013087: Zinc finger C2H2-type (domain) [99-723]
- IPR036236: Zinc finger C2H2 superfamily (homologous_superfamily) [304-639]
Interaction partners: - Homeobox protein ceh-6
- Egg-laying defective protein 27
- Homeobox protein egl-5
- Transcription factor egl-46
- Probable histone-binding protein lin-53
- Transcription factor sox-2
- Probable histone-binding protein rba-1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0019222 (regulation of metabolic process), GO:0007389 (pattern specification process), GO:0003006 (developmental process involved in reproduction), GO:0009892 (negative regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0048523 (negative regulation of cellular process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0030154 (cell differentiation), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0060255 (regulation of macromolecule metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0090598 (male anatomical structure morphogenesis), GO:0045165 (cell fate commitment), GO:0009887 (animal organ morphogenesis), GO:0048598 (embryonic morphogenesis), GO:0003002 (regionalization), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0009790 (embryo development), GO:0007548 (sex differentiation), GO:0080090 (regulation of primary metabolic process), GO:0046661 (male sex differentiation), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0048562 (embryonic organ morphogenesis), GO:0007423 (sensory organ development), GO:0090596 (sensory organ morphogenesis), GO:0045138 (nematode male tail tip morphogenesis), GO:0010468 (regulation of gene expression), GO:0022008 (neurogenesis), GO:0048568 (embryonic organ development), GO:0007399 (nervous system development), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0051253 (negative regulation of RNA metabolic process), GO:0030182 (neuron differentiation), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0009952 (anterior/posterior pattern specification), GO:0048592 (eye morphogenesis), GO:0001654 (eye development), GO:0048048 (embryonic eye morphogenesis), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0150063 (visual system development), GO:0048699 (generation of neurons), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045892 (negative regulation of DNA-templated transcription), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0000122 (negative regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)