Protein: Sensor histidine kinase BtsS (P0AD14)
Organism: Escherichia coli (strain K12)
Function: Member of the two-component regulatory system BtsS/BtsR, which is part of a nutrient-sensing regulatory network composed of BtsS/BtsR, the low-affinity pyruvate signaling system YpdA/YpdB and their respective target proteins, BtsT and YhjX. Responds to depletion of nutrients, specifically serine, and the concomitant presence of extracellular pyruvate. BtsS is a high-affinity receptor for extracellular pyruvate that activates BtsR by phosphorylation. Activation of the BtsS/BtsR signaling cascade also suppresses YpdA/YpdB-mediated yhjX induction
Subcellular location: Cell inner membrane; Multi-pass membrane protein
Sequence: MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKFLCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSMGGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILAIARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAASTEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSTYTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNRLFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIKAVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQVNIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNAGLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA
InterPro domains: - IPR003018: GAF domain (domain) [218-365]
- IPR050640: Bacterial two-component sensor kinase systems (family) [36-560]
- IPR036890: Histidine kinase/HSP90-like ATPase superfamily (homologous_superfamily) [423-557]
- IPR011620: Signal transduction histidine kinase, 5TM receptor LytS, transmembrane region (domain) [27-196]
- IPR010559: Signal transduction histidine kinase, internal region (domain) [371-448]
- IPR029016: GAF-like domain superfamily (homologous_superfamily) [231-356]
Interaction partners: - Transcriptional regulatory protein-like BtsR
- Transcriptional regulatory protein BtsR
- Transcriptional regulatory protein BtsR
- Transcriptional regulatory protein BtsR
- Transcriptional regulatory protein YpdB
- Transcriptional regulatory protein YpdB
- Transcriptional regulatory protein YpdB
- Pyruvate/proton symporter BtsT
- Uncharacterized MFS-type transporter YhjX
- Uncharacterized protein YehS
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009605 (response to external stimulus), GO:0051716 (cellular response to stimulus), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0006793 (phosphorus metabolic process), GO:0009991 (response to extracellular stimulus), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0043170 (macromolecule metabolic process), GO:0036211 (protein modification process), GO:0006796 (phosphate-containing compound metabolic process), GO:0043412 (macromolecule modification), GO:0031667 (response to nutrient levels), GO:0006468 (protein phosphorylation), GO:0018193 (peptidyl-amino acid modification), GO:0016310 (phosphorylation), GO:0018202 (peptidyl-histidine modification)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)