Protein: MMS19 nucleotide excision repair protein (Q95U54)
Organism: Drosophila melanogaster (Fruit fly)
Function: Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity (By similarity). In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins (By similarity). Essential for diploid cell cycles, organ growth and development. Regulates mitosis by binding to Xpd and thereby competing with the Xpd-mediated repression on the Cdk-activating kinase (CAK) complex. Regulates the centrosomal localization of the MT regulator tacc, a downstream target of aurA kinase. Binds to microtubules (MT). Regulates spindle and astral MT growth, MT stability and bundling. In neuroblasts, necessary for timely and coordinated spindle assembly and orientation which is necessary for mitotic progression. In young embryos, the maternal protein is important for progression through mitosis.
Subcellular location: Cytoplasm, cytoskeleton, spindle {ECO:0000255|RuleBase:RU367072, ECO:0000269|PubMed:29361561}. Nucleus {ECO:0000255|RuleBase:RU367072, ECO:0000269|PubMed:29361561}. Cytoplasm {ECO:0000269|PubMed:29361561}. Midbody {ECO:0000269|PubMed:29361561}. Note=Localizes to the nucleus at the beginning of metaphase. {ECO:0000269|PubMed:29361561}.
Sequence: MTTPTRATLEKALKSDQKLVNSATQIAKDLTAKAYDISALAEALGFALSSPDMEERVAGTNLLSAVLIALPQDLLQERQLEFLSTFYMDRLRDHHNVMPAIIDGIDALVHMKALPRAQIPQILQSFFEHTTCQSQTRSDRTKLFHIFQYLTENFQDELQAMAGDFVYGLINSIDGERDPRNLDIIFSFMPEFLSTYPLLHLAEEMFEIFACYFPIDFNPSKQDPAAITRDELSKKLTNCLVANNEFAEGTVVLAIEKLESELLVAKLDSIELLHQAAVKFPPSVLEPHFDQIWQALKTETFPGNDNEEILKASLKALSALLERAAHIPDISHSYQSSILGVILPHLSDVNQRLFHPATGIALVCVSGDAPYAADKILNSFLLKLQAADASSEQRIKIYYIVSQVYKLSALRGSLQKLDTTIRESVQDDVIASLRLIEQEEFDAKKEDLELQKAALSVLNESAPLLNEKQRALIYKALVQLVSHPSIDIDFTTLTVSLGALQPVEVQSNFIDVCVRNFEIFSTFVKRKIYTNLLPLMPQIAFTQRILDLVMTQTFNDTTAEPVRLLALEALNKLLLLADQRFIVDVQQESNLLHKLIELGQKTEGLSMQSLEQIAGALSRITQQLPLSEQSAIVSEYLPGLNLSQSADLYITKGLLGYLHKDITLDDHFERLLTDLTQLSLNSDNEQLRVIAHHLLCSMVNKMESNPANLRKVKKITEQLKVAIKKGDVRAVEILAWVGKGLVVAGFDEAADVVGDLSDLLKHPSLSTAAALGFDIIAAEYPELDLPVVKFLYKQKLFHTIMGKMGSKLANYCVHHLKAFVYVLKATPQAVIKLNIEQLGPLLFKSLEEHNEAQSLCIALGICEKFVAQQDTYFQGHLAHLIPSCLELSKYKAQHTMQVRIAALQLLYDVTKYPTFVLLPHKVDVTLALAAALDDPKRLVRNTAVKARNAWYLVGAPSPN
InterPro domains: - IPR016024: Armadillo-type fold (homologous_superfamily) [35-946]
- IPR024687: MMS19, C-terminal (domain) [644-908]
- IPR029240: MMS19, N-terminal (domain) [42-301]
- IPR039920: DNA repair/transcription protein MMS19 (family) [10-954]
- IPR011989: Armadillo-like helical (homologous_superfamily) [606-947]
Interaction partners: - MIP18 family protein galla-2
- Probable cytosolic iron-sulfur protein assembly protein Ciao1
- General transcription and DNA repair factor IIH helicase subunit XPD
- MIP18 family protein galla-1
- ADP/ATP translocase
- Probable cytosolic Fe-S cluster assembly factor CG17683
- Regulator of telomere elongation helicase 1 homolog
- DNA polymerase epsilon catalytic subunit 1
- General transcription and DNA repair factor IIH helicase/translocase subunit XPB
- DNA polymerase delta catalytic subunit
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0071840 (cellular component organization or biogenesis), GO:0009892 (negative regulation of metabolic process), GO:0019222 (regulation of metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0016043 (cellular component organization), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0006325 (chromatin organization), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression), GO:0040029 (epigenetic regulation of gene expression), GO:0006338 (chromatin remodeling), GO:0045814 (negative regulation of gene expression, epigenetic), GO:0031507 (heterochromatin formation), GO:0070828 (heterochromatin organization), GO:0140719 (constitutive heterochromatin formation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)