Protein: Kelch-like ECH-associated protein 1B (A0A2R8Q1W5)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting nfe2l2/nrf2 for ubiquitination. Keap1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of nfe2l2/nrf2, a transcription factor regulating expression of many cytoprotective genes. In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting nfe2l2/nrf2 nuclear accumulation and expression of phase II detoxifying enzymes (By similarity).
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:Q9Z2X8}. Nucleus {ECO:0000250|UniProtKB:Q9Z2X8}. Note=Mainly cytoplasmic. {ECO:0000250|UniProtKB:Q9Z2X8}.
Sequence: MTECKAEVTPSASNGHRVFSYTLESHTAAAFAIMNELRRERQLCDVTLRVRYCPLDTHVDFVAHKVVLASSSPVFRAMFTNGLKECGMEVVPIEGIHPKVMGRLIEFAYTASISVGEKCVIHVMNGAVMYQIDSVVQACCDFLVEQLDPSNAIGIASFAEQIGCTELHQKAREYIYMNFSQVATQEEFFTLSHCQLVTLISRDELNVRCESEVFHACVAWVQYDREERRPYVQALLQAVRCHSLTPHFLQRQLEHFEWDAQSKDYLSQIFRDLTLHKPTKVIPLRTPKVPQLIYTVGGYFRQSLSFLEAFNPCSGAWLRLADLQVPRSGLAACVISGLLYAVGGRNNGPDGNMDSHTLDCYNPMNNCWRPCAHMSVPRNRIGVGVIDGMIYAVGGSHGCTHHNSVERYDPERDSWQLVSPMLTRRIGVGVAVINRLLYAVGGFDGTHRLSSAECYNPERDEWRSIAAMNTVRSGAGVCALGNYIYVMGGYDGTNQLNTVERYDVEKDSWSFSASMRHRRSALGVTTHHGRIYVLGGYDGNTFLDSVECFDPETDSWTEVTHMKSGRSGVGVAVTMEPCHKELIPCQC
InterPro domains: - IPR011705: BTB/Kelch-associated (domain) [152-254]
- IPR030563: Kelch-like ECH-associated protein 1, BTB/POZ domain (domain) [23-148]
- IPR006652: Kelch repeat type 1 (repeat) [292-576]
- IPR047098: Kelch-like ECH-associated protein 1, BACK domain (domain) [146-236]
- IPR000210: BTB/POZ domain (domain) [34-147]
- IPR011333: SKP1/BTB/POZ domain superfamily (homologous_superfamily) [12-148]
- IPR015915: Kelch-type beta-propeller (homologous_superfamily) [274-587]
- IPR017096: BTB-kelch protein (family) [1-581]
Interaction partners: - Cullin 3
- Nuclear factor
- Nuclear factor, erythroid 2-like 2b.
- Kelch-like protein 9 isoform X1
- Kelch-like family member 13
- E3 ubiquitin-protein ligase RBX1
- Rho-related BTB domain-containing 1.
- Rho-related BTB domain-containing 2b.
- Rho-related BTB domain-containing 2a.
- Kelch-like ECH-associated protein 1A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0042802 (identical protein binding), GO:0019904 (protein domain specific binding), GO:0097718 (disordered domain specific binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0006979 (response to oxidative stress), GO:0043170 (macromolecule metabolic process), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0070647 (protein modification by small protein conjugation or removal), GO:0032446 (protein modification by small protein conjugation), GO:0016567 (protein ubiquitination), GO:0051865 (protein autoubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0140535 (intracellular protein-containing complex), GO:1990234 (transferase complex), GO:0000151 (ubiquitin ligase complex), GO:0031461 (cullin-RING ubiquitin ligase complex), GO:0031463 (Cul3-RING ubiquitin ligase complex)