Protein: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase sqv-8 (EC 2.4.1.135) (Glucuronyl transferase I) (GlcAT-I) (Squashed vulva protein 8) (Vulval invagination protein sqv-8) (Q09363)
Organism: Caenorhabditis elegans
Function: Glycosyltransferase required for the biosynthesis of the tetrasaccharide (GlcA-Gal-Gal-Xyl-)Ser core linker of heparan sulfate and chondroitin sulfate. May be involved in the biosynthesis of the HNK-1 carbohydrate epitope on glycoproteins. Required for embryonic development. Involved in the elongation of the pharyngeal isthmus during the later stages of embryonic development. Involved in vulval epithelium invagination.
Subcellular location: Membrane {ECO:0000269|PubMed:11005858}; Single-pass type II membrane protein {ECO:0000305}.
Sequence: MFPSRLLEKWWLRAFIALVIFFVWQLFYAINRVQSLEEERATLQATIEVLTRKSDGLRTQIFEKERNLVRLNGKVEEIDTQIRDHLSLLPRVNRSTPFIYFITPTHFRAAQRADLTRLSYTLSHVPNLHWIVVEDSDELTPSIAGILKRSKIPNTHLNARTPSDQKMRYDDPNWTLPRGVEQRNRALLWIQNQLSGVKEGVVYFGDDDNTYDLKIFGEMRKVKNAGVWPVGIVGGMFVETPILEKNGSISHFNAVWKPERPFPIDMAAFAVNISLVLSNANALFSFDVPRGYQESTFLENLGIHRYNMEPLAEMCTKVYVWHTRTEKPKLSKESIDRLTKKTGFNSLEAHALGVDN
InterPro domains: - IPR029044: Nucleotide-diphospho-sugar transferases (homologous_superfamily) [97-348]
- IPR005027: Glycosyl transferase, family 43 (family) [11-336]
Interaction partners: - Beta-1,3-galactosyltransferase sqv-2
- Xylosylprotein 4-beta-galactosyltransferase
- Acid phosphatase-like protein 2
- UDP-glucuronate decarboxylase
- Coiled-coil domain-containing protein 130 homolog
- UDP-sugar transporter sqv-7
- Exostosin-like-3 homolog
- Chondroitin sulfate synthase mig-22
- Putative steroid dehydrogenase 4
- CYtochrome P450 family
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016757 (glycosyltransferase activity), GO:0016758 (hexosyltransferase activity), GO:0008194 (UDP-glycosyltransferase activity), GO:0015020 (glucuronosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0048522 (positive regulation of cellular process), GO:0032879 (regulation of localization), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0071704 (organic substance metabolic process), GO:0051050 (positive regulation of transport), GO:0044238 (primary metabolic process), GO:1903829 (positive regulation of protein localization), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0051049 (regulation of transport), GO:0032388 (positive regulation of intracellular transport), GO:0060341 (regulation of cellular localization), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0051222 (positive regulation of protein transport), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0090316 (positive regulation of intracellular protein transport), GO:0051223 (regulation of protein transport), GO:0009100 (glycoprotein metabolic process), GO:0044272 (sulfur compound biosynthetic process), GO:1904951 (positive regulation of establishment of protein localization), GO:0070201 (regulation of establishment of protein localization), GO:0032880 (regulation of protein localization), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0006022 (aminoglycan metabolic process), GO:0015012 (heparan sulfate proteoglycan biosynthetic process), GO:0006029 (proteoglycan metabolic process), GO:0030203 (glycosaminoglycan metabolic process), GO:0009101 (glycoprotein biosynthetic process), GO:0033157 (regulation of intracellular protein transport), GO:0006023 (aminoglycan biosynthetic process), GO:0050654 (chondroitin sulfate proteoglycan metabolic process), GO:0006024 (glycosaminoglycan biosynthetic process), GO:0030166 (proteoglycan biosynthetic process), GO:0050650 (chondroitin sulfate proteoglycan biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)