Protein: D-alanyl-D-alanine carboxypeptidase DacC (DD-carboxypeptidase) (DD-peptidase) (EC 3.4.16.4) (Penicillin-binding protein 6) (PBP-6) (P08506)
Organism: Escherichia coli (strain K12)
Function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.
Subcellular location: Cell inner membrane {ECO:0000269|PubMed:3330754}; Peripheral membrane protein {ECO:0000269|PubMed:3330754}; Periplasmic side {ECO:0000269|PubMed:3330754}. Note=N-terminus lies in the periplasmic space, targeted there by the C-terminal amphiphilic helix (PMID:3330754).
Sequence: MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWILMDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGNPALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAKKLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQPNRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEKLLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRVWDFVMMKFHQWFGSWFS
InterPro domains: - IPR037167: D-Ala-D-Ala carboxypeptidase, C-terminal domain superfamily (homologous_superfamily) [285-376]
- IPR015956: Penicillin-binding protein, C-terminal domain superfamily (homologous_superfamily) [285-378]
- IPR001967: Peptidase S11, D-alanyl-D-alanine carboxypeptidase A, N-terminal (domain) [29-266]
- IPR012907: Peptidase S11, D-Ala-D-Ala carboxypeptidase A, C-terminal (domain) [285-376]
- IPR012338: Beta-lactamase/transpeptidase-like (homologous_superfamily) [14-285]
- IPR018044: Peptidase S11, D-alanyl-D-alanine carboxypeptidase A (family) [65-164]
Interaction partners: - D-alanyl-D-alanine carboxypeptidase DacB
- Putative D-alanyl-D-alanine carboxypeptidase
- Penicillin-binding protein 1A
- Peptidoglycan D,D-transpeptidase MrdA
- D-alanyl-D-alanine carboxypeptidase DacD
- D-alanyl-D-alanine carboxypeptidase DacA
- Penicillin-binding protein 1B
- Probable L,D-transpeptidase YcbB
- Peptidoglycan D,D-transpeptidase FtsI
- Penicillin-binding protein 1C
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:1901681 (sulfur compound binding), GO:0097159 (organic cyclic compound binding), GO:0033218 (amide binding), GO:0140096 (catalytic activity, acting on a protein), GO:0043167 (ion binding), GO:0008233 (peptidase activity), GO:0016810 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds), GO:0043177 (organic acid binding), GO:0017171 (serine hydrolase activity), GO:0043168 (anion binding), GO:0008236 (serine-type peptidase activity), GO:0031406 (carboxylic acid binding), GO:0008238 (exopeptidase activity), GO:0016812 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides), GO:0070008 (serine-type exopeptidase activity), GO:0004180 (carboxypeptidase activity), GO:0008800 (beta-lactamase activity), GO:0033293 (monocarboxylic acid binding), GO:0004185 (serine-type carboxypeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050793 (regulation of developmental process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0022603 (regulation of anatomical structure morphogenesis), GO:0022604 (regulation of cell morphogenesis), GO:0006022 (aminoglycan metabolic process), GO:0008360 (regulation of cell shape), GO:0030203 (glycosaminoglycan metabolic process), GO:0000270 (peptidoglycan metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0098552 (side of membrane), GO:0009986 (cell surface), GO:0031975 (envelope), GO:0071944 (cell periphery), GO:0042597 (periplasmic space), GO:0016020 (membrane), GO:0009897 (external side of plasma membrane), GO:0030288 (outer membrane-bounded periplasmic space), GO:0005886 (plasma membrane), GO:0030313 (cell envelope)