Protein: Elongator complex protein 4 (Q9VMQ7)
Organism: Drosophila melanogaster (Fruit fly)
Function: Component of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. Binding by the elongator complex stabilizes microtubules and promotes their growth. This induces central spindle asymmetry, promoting polarized signaling endosome trafficking during asymmetric cell division and cell fate assignation of sensory organ precursor cells
Subcellular location: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=During asymmetric cell division of sensory organ precursor cells the elongator complex preferentially binds and stabilizes microtubules on the anterior side (pIIb daughter cell) of the spindle
Sequence: MTSFRKRTVQKPIRGTRTSPHTAQVITSSGNPYLDVVIGGGLPMGSICLIEEDRFMTHAKVLAKYFLAEGVISKQEIFLGSLDDIPAEMLRRLPRPLTDQESMEQSEVQALGDAGAENGLRIAWRYNDLPLVNSEHATAKIGHHFNLMEQMDSMMLYNVKTTLWDDSPKHLDIVIDEEFSKSSSPTTPSLEQQPVEDAPPIPGTETAPQEKMPAQEEENSANNNNNNNNNSSSVTSSTKTGSQDSPLQVFHNPRYKGLLNDIQQLLRNESFVAGTKNNLCRVCLTSLGSPLWYDEHFGEDLIKFLTLLMASVRNCNSVCLITMPMHLIAKYDASLVPKIRQLVDYAIELESFAGSERETHPAFKEYSGLLHLHKMSAINTLAVHMPETPDLAFKLRRKKFIIEKFHLPPELQESSAKPDNCISGLLSNSNATASLDF
InterPro domains: - IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [8-415]
- IPR008728: Elongator complex protein 4 (family) [1-437]
Interaction partners: - Elongator complex protein 3
- Elongator complex protein 5
- Elongator complex protein 1
- Elongator complex protein 2
- Elongator complex protein 6
- Diphthamide biosynthesis protein 3
- Adenylyltransferase and sulfurtransferase MOCS3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0043473 (pigmentation), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0042440 (pigment metabolic process), GO:0033060 (ocellus pigmentation), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0043474 (pigment metabolic process involved in pigmentation), GO:0048066 (developmental pigmentation), GO:0044550 (secondary metabolite biosynthetic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0043324 (pigment metabolic process involved in developmental pigmentation), GO:0046483 (heterocycle metabolic process), GO:0019538 (protein metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0046148 (pigment biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0031323 (regulation of cellular metabolic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0048069 (eye pigmentation), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0016063 (rhodopsin biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0042441 (eye pigment metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006726 (eye pigment biosynthetic process), GO:0006996 (organelle organization), GO:0006726 (eye pigment biosynthetic process), GO:0010468 (regulation of gene expression), GO:0016050 (vesicle organization), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0009658 (chloroplast organization), GO:1903506 (regulation of nucleic acid-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)