Protein: Caffeine dehydrogenase subunit beta (EC 1.17.5.2) (Caffeine dehydrogenase medium subunit) (D7REY4)
Organism: Pseudomonas sp. (strain CBB1)
Function: Catalyzes the hydrolitical oxidation of 1,3,7-trimethylxanthine (caffeine) by incorporation of an oxygen atom originating from a water molecule into position C-8 to produce 1,3,7-trimethyluric acid (TMU). Coenzyme Q0 is the preferred electron acceptor and coenzyme Q2 can also be used, but oxygen and NADP cannot.
Sequence: MKPTAFDYIRPTSLPEALAILAEHSDDVAILAGGQSLMPLLNFRMSRPALVLDINDISELQQVRCENDTLYVGSMVRHCRVEQEEIFRSTIPLMSEAMTSVAHIQIKTRGTLGGNLCNAHPASEMPAVITALGASMVCKSEKRGERVLTPEEFFEGALQNGLQSDELLCEIRIPVPSQYVGWAFEEVARRHGDFAQCGAAVLIGAEDRKIDYARIALCSIGETPIRFHALEQWLIGRPVGNDLPADVKLHCREILDVAEDSTMTAENRAKLASAVTSRAIARAADRIVHLDVKRG
InterPro domains: - IPR016167: FAD-binding, type PCMH, subdomain 1 (homologous_superfamily) [1-56]
- IPR002346: Molybdopterin dehydrogenase, FAD-binding (domain) [5-175]
- IPR016166: FAD-binding domain, PCMH-type (domain) [1-178]
- IPR016169: FAD-binding, type PCMH, subdomain 2 (homologous_superfamily) [58-176]
- IPR005107: CO dehydrogenase flavoprotein, C-terminal (domain) [182-283]
- IPR051312: Diverse Substrate Oxidoreductases (family) [1-285]
- IPR036318: FAD-binding, type PCMH-like superfamily (homologous_superfamily) [1-175]
- IPR036683: CO dehydrogenase flavoprotein, C-terminal domain superfamily (homologous_superfamily) [182-286]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016903 (oxidoreductase activity, acting on the aldehyde or oxo group of donors)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0044248 (cellular catabolic process), GO:0046700 (heterocycle catabolic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0009112 (nucleobase metabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0072521 (purine-containing compound metabolic process), GO:0019439 (aromatic compound catabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0006144 (purine nucleobase metabolic process), GO:0072523 (purine-containing compound catabolic process), GO:0046113 (nucleobase catabolic process), GO:0006145 (purine nucleobase catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)