Protein: DASH complex subunit DAD3 (Q59VC6)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Component of the DASH complex that connects microtubules with kinetochores and couples microtubule depolymerisation to chromosome movement; it is involved in retrieving kinetochores to the spindle poles before their re-orientation on the spindle in early mitosis and allows microtubule depolymerization to pull chromosomes apart and resist detachment during anaphase. Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and microtubules. Kinetochores also serve as an input point for the spindle assembly checkpoint, which delays anaphase until all chromosomes have bioriented on the mitotic spindle
Subcellular location: Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore
Sequence: MSFTTKDKSDDSSIITTNYADNPNLSPIEQKILQQYQLMNNNLIKVSNELELLTNTTDEFGKGKGSSIHLVENLRQLETKLVFVYTFFKGAVYSILNAQDYIAEQETNGLEETNEEEDGNEEDESGNVELSGVSADEN
InterPro domains: - IPR013965: DASH complex subunit Dad3 (family) [11-117]
Interaction partners: - DASH complex subunit SPC34
- DASH complex subunit DAD2
- DASH complex subunit SPC19
- DASH complex subunit DAD4
- DASH complex subunit DAM1
- DASH complex subunit ASK1
- DASH complex subunit DUO1
- DASH complex subunit DAD1
- Actin-related protein 5
- Spindle pole body component
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0008092 (cytoskeletal protein binding), GO:0015631 (tubulin binding), GO:0008017 (microtubule binding), GO:0051010 (microtubule plus-end binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:0016043 (cellular component organization), GO:0007059 (chromosome segregation), GO:1903047 (mitotic cell cycle process), GO:0000278 (mitotic cell cycle), GO:0098813 (nuclear chromosome segregation), GO:0006996 (organelle organization), GO:0000070 (mitotic sister chromatid segregation), GO:0140014 (mitotic nuclear division), GO:0048285 (organelle fission), GO:0000819 (sister chromatid segregation), GO:0051276 (chromosome organization), GO:0000280 (nuclear division)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098687 (chromosomal region), GO:0000940 (outer kinetochore), GO:0140513 (nuclear protein-containing complex), GO:0099080 (supramolecular complex), GO:0042729 (DASH complex), GO:0000776 (kinetochore), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000775 (chromosome, centromeric region), GO:0043228 (non-membrane-bounded organelle), GO:0000779 (condensed chromosome, centromeric region), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome), GO:0005634 (nucleus), GO:0000793 (condensed chromosome)