Protein: G1/S-specific cyclin-D3 (P48961)
Organism: Rattus norvegicus (Rat)
Function: Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Shows transcriptional coactivator activity with ATF5 independently of CDK4.
Subcellular location: Nucleus {ECO:0000250|UniProtKB:P30281}. Cytoplasm {ECO:0000250|UniProtKB:P30281}.
Sequence: MELLCCEGTRLAPRAGPDPRLLGDQRVLQSLLRLEERYVPRGSYFQCVQKEIKPHMRKMLAYWMLEVCEEQRCEEDVFPLAMNYLDRYLSCVPTRKAQLQLLGTVCLLLASKLRETTPLTIEKLCIYTDQAMAPWQLREWEVLVLGKLKWDLAAVIAHDFLALILHRLSLPSDRQALVKKHAQTFLALCATDYTFAMYPPSMIATGSIGAAVLGLGACSMSADELTELLAGITGTEVDCLRACQEQQIEAALRESLREAAQTAPSPVPKAPGGSSSQGPSQTSTPTDVTAIHL
InterPro domains: - IPR048258: Cyclins, cyclin-box (conserved_site) [57-88]
- IPR004367: Cyclin, C-terminal domain (domain) [155-281]
- IPR013763: Cyclin-like domain (domain) [62-146]
- IPR039361: Cyclin (family) [42-243]
- IPR006671: Cyclin, N-terminal (domain) [27-152]
- IPR036915: Cyclin-like superfamily (homologous_superfamily) [23-250]
Interaction partners: - cyclin-dependent kinase
- Cyclin-dependent kinase 4
- Cyclin-dependent kinase 2
- Cyclin-dependent kinase inhibitor 1B
- Cyclin-dependent kinase inhibitor 1
- Cyclin-dependent kinase 4 inhibitor C
- Cyclin-dependent kinase inhibitor 1C
- Retinoblastoma-associated protein
- Retinoblastoma-like protein 2
- M-phase inducer phosphatase 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0044877 (protein-containing complex binding), GO:0005515 (protein binding), GO:0019899 (enzyme binding), GO:0019900 (kinase binding), GO:0019901 (protein kinase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0048522 (positive regulation of cellular process), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0065009 (regulation of molecular function), GO:0051726 (regulation of cell cycle), GO:0050790 (regulation of catalytic activity), GO:0044093 (positive regulation of molecular function), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0045787 (positive regulation of cell cycle), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0010562 (positive regulation of phosphorus metabolic process), GO:1904029 (regulation of cyclin-dependent protein kinase activity), GO:0051246 (regulation of protein metabolic process), GO:0045737 (positive regulation of cyclin-dependent protein serine/threonine kinase activity), GO:0051247 (positive regulation of protein metabolic process), GO:0051338 (regulation of transferase activity), GO:0043085 (positive regulation of catalytic activity), GO:0051174 (regulation of phosphorus metabolic process), GO:0000079 (regulation of cyclin-dependent protein serine/threonine kinase activity), GO:0043549 (regulation of kinase activity), GO:0051347 (positive regulation of transferase activity), GO:0031399 (regulation of protein modification process), GO:0045937 (positive regulation of phosphate metabolic process), GO:0031401 (positive regulation of protein modification process), GO:1904031 (positive regulation of cyclin-dependent protein kinase activity), GO:0019220 (regulation of phosphate metabolic process), GO:0042325 (regulation of phosphorylation), GO:0001932 (regulation of protein phosphorylation), GO:0033674 (positive regulation of kinase activity), GO:0042327 (positive regulation of phosphorylation), GO:0001934 (positive regulation of protein phosphorylation), GO:0045859 (regulation of protein kinase activity), GO:0071900 (regulation of protein serine/threonine kinase activity), GO:0045860 (positive regulation of protein kinase activity), GO:0071902 (positive regulation of protein serine/threonine kinase activity)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)