Protein: Multifunctional protein pyr-1 [Includes: Glutamine-dependent carbamoyl-phosphate synthase (EC 6.3.5.5); Glutamine amidotransferase (GATase) (GLNase) (EC 3.5.1.2); Ammonium-dependent carbamoyl phosphate synthase (CPS) (CPSase) (EC 6.3.4.16); Aspartate carbamoyltransferase (EC 2.1.3.2); Dihydroorotase (EC 3.5.2.3)] (Q18990)
Organism: Caenorhabditis elegans
Function: This protein is a 'fusion' protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase) (By similarity). Involved in the elongation of the pharyngeal isthmus during development, probably by providing precursors of UDP-sugars required for heparan sulfate proteoglycan biosynthesis. Regulates the organization of the actin and intermediate filaments cytoskeleton in the pharyngeal muscles.
Sequence: MRATLHLEDGSTFVGSIYGATKSVVGEIVFQTGMVGYVESLTDPSYAKQLLTLTYPLIGNYGVPSAEILDQFKLPAEFESDRIWPAALIVEKICVDGEHSHWQAVQSLSEWLRKADVPCLSGIDVRQLVKKIRETGTMKAKLVIESDNAQNFDYVDVNAENLVDFVSRKEPVVYGSGDQTILAVDCGLKNNQIRCLAKRGFRVKVVPWNHPIDTESDYDGLFLSNGPGDPEICAPLVDRLAKVIARGDKPIFGICLGHQILSRAIGAKTYKLKYGNRGHNQPCTHYATGRCYITSQNHGYAVDPDSLPADWKALFTNENDKTNEGIVHSSKPFFSVQFHPEHTAGPTDCEFLFDVFADSVRQAKSGTFMNVDQELTRLMTFTPIYHAKEQRKVLVLGSGGLTIGQAGEFDYSGAQALKALREEGIRTVLINPNIATVQTSKGFADFTYFLPITKEYVTDVIKKERPTGILCTFGGQTALNCAIDLYKDGIFEQYDVQVLGTQINTIMKTEDRDLFNQEISAIGEKVAPSKAATTMEGAIEAAEELGYPVLVRAAYALGGLGSGFADNREELIAIAQQALAHSNQVLVDKSLKGWKEVEYEVVRDAYDNCITVCNMENVDPLGIHTGESVVVAPSQTLSDREYNALRTCAIKVIRHLGIIGECNIQYALDPYSLTYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGQHLPVIRNSVTGTTTACFEPSLDYCVVKIPRWDLGKFARVSTQIGSSMKSVGEVMGIGRCFEEALQKALRMVSDHADGFSPYTFSRPTTADDLSKPTDKRMFALARGMYYGDFDVEKAHELTRIDRWFLFRMQNIVDIYHRLEKTDVNTVSAELLLEAKQAGFSDRQIAKKIGSNEYTVREARFVKGITPCVKQIDTVAGEWPAQTNYLYTTFNGIENDVSFNMKNAVMVLGSGVYRIGSSVEFDSSCVGCIRELKALGYSTITVNCNPETVSTDYDICDRLYFEEISFETVLDVYHLEKPKGVILAFGGQAPNNIAMSLSRAQVKIFGTSPNDIDNAEDRFKFSRKLESLKISQPQWKKSENMEDAKNFCAQVGYPCLIRPSYVLSGAAMNVAHNAEDLEVFLKQAAVVAKEHPVVVSKFINEAKELDVDAVALDGKLVVMAVSEHIENAGVHSGDATLVTPAQDMNKLTLDRIKDITFRIAEAFNVNGPFNMQLIAKNNELKVIECNLRVSRSFPFVSKTLDYDFVALATRAMMASDSPAIRATIKPTATLLKGKGRVGVKVPQFSFSRLAGADVMLGVEMASTGEVACFGTSRCDAYLKALLSTGFVVPKQNIFISIGGYHAKAEMLKSVEALLKLGYELYGSKGTADYFQSNKINVKPVDWPFEEGSSDEKTASGTRSVVEFLENKEFHLVINLPIRGSGAYRVSAFRTHGYKTRRMAIDNGIPLITDIKCAKTFIQALEMVGKRPTMNSLVDCVTSKSLKRLPGMVDIHVHVREPGATHKEDWATCSKAALAGGVTTILAMPNTSPVLVDTDSFYQTEQLASAKSVVDYALYIGATPNNSKFAAEFADKAAGLKMYLNETFSTLKMDNISDWAKHLSAFPANRPIVCHAEKQTLAAILCMAQMANRAVHIAHVATADEINLVKEAKQRGWNVTCEVCPHHLFLIEEDLPDGIREVRPRLVKPEDRQALWDNMEYIDCFATDHAPHTWAEKTGKDGKIPPGFPGVEYMLPLLLTAVHDGKLTMKELTDRMSTNPRRIFNLPPQDDTYIEVDLNEEWTIPENGGQSKAGWTPFAGRKVFGKVHNVIIRGEEAVIDGRIVAIPGFGKNVRLYPHSGTAHRGDSDFDQILEPIPQQMIESSSDEQSPLHTPPRAHTPIAFPGELLAKNCISVKHLDKGQINRIFELADRYKHDVEKGHPLTHILNGKVLVNLFYEVSTRTSCSFSAAMQRLGGSVISVDSQSSSVQKGETLEDTVQVLGSYGDILVLRSNENGAADRAARVCDQPVINGGDGTGEHPTQALLDVYTIRQEMGTVNGLTIALVGDLKNGRTVHSLAKLLCLYKDITLHYVAPSTELEMPQEVLDYVSSKSNFVQKKFTSLAEGINHVDVVYVTRIQKERFSSPDEYNKVKGSYVINAKLLNEAARDVEEPSSLLVPARSLPIVMHPLPRVDEIAVELDHDERAAYFRQAKNGVFVRMSILSLLLGRGHL
InterPro domains: - IPR011607: Methylglyoxal synthase-like domain (domain) [1318-1474]
- IPR002082: Aspartate carbamoyltransferase (family) [1876-2195]
- IPR006680: Amidohydrolase-related (domain) [1476-1754]
- IPR006274: Carbamoyl-phosphate synthase, small subunit (family) [1-364]
- IPR029062: Class I glutamine amidotransferase-like (homologous_superfamily) [154-364]
- IPR016185: Pre-ATP-grasp domain superfamily (homologous_superfamily) [389-1050]
- IPR005483: Carbamoyl phosphate synthase, CPSase domain (domain) [402-777]
- IPR005479: Carbamoyl phosphate synthase, ATP-binding domain (domain) [511-1242]
- IPR006131: Aspartate/ornithine carbamoyltransferase, Asp/Orn-binding domain (domain) [2026-2191]
- IPR002195: Dihydroorotase, conserved site (conserved_site) [1481-1704]
- IPR013815: ATP-grasp fold, subdomain 1 (homologous_superfamily) [1062-1131]
- IPR006130: Aspartate/ornithine carbamoyltransferase (family) [1923-2181]
- IPR011761: ATP-grasp fold (domain) [516-1245]
- IPR005480: Carbamoyl-phosphate synthetase, large subunit oligomerisation domain (domain) [798-922]
- IPR011059: Metal-dependent hydrolase, composite domain superfamily (homologous_superfamily) [1757-1826]
- IPR006132: Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding (domain) [1877-2019]
- IPR006275: Carbamoyl-phosphate synthase, large subunit (family) [388-1452]
- IPR032466: Metal-dependent hydrolase (homologous_superfamily) [1478-1757]
- IPR002474: Carbamoyl-phosphate synthase small subunit, N-terminal domain (domain) [1-143]
- IPR017926: Glutamine amidotransferase (domain) [183-357]
Interaction partners: - Dihydroorotate dehydrogenase
- glutamate synthase
- Amidophosphoribosyltransferase
- Probable Golgi transport protein 1
- Dihydropyrimidine dehydrogenase [NADP
- Adenylosuccinate synthetase
- Cytochrome-b5 reductase
- Uridine 5'-monophosphate synthase
- Trifunctional purine biosynthetic protein adenosine-3
- Dihydropyrimidinase 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0016740 (transferase activity), GO:0016787 (hydrolase activity), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0016810 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds), GO:0016879 (ligase activity, forming carbon-nitrogen bonds), GO:0016743 (carboxyl- or carbamoyltransferase activity), GO:0016884 (carbon-nitrogen ligase activity, with glutamine as amido-N-donor), GO:0016812 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0006082 (organic acid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0009112 (nucleobase metabolic process), GO:0043436 (oxoacid metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0072527 (pyrimidine-containing compound metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0046112 (nucleobase biosynthetic process), GO:0009064 (glutamine family amino acid metabolic process), GO:0006206 (pyrimidine nucleobase metabolic process), GO:0072528 (pyrimidine-containing compound biosynthetic process), GO:0006541 (glutamine metabolic process), GO:0019856 (pyrimidine nucleobase biosynthetic process), GO:0006207 ('de novo' pyrimidine nucleobase biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0055120 (striated muscle dense body), GO:0030017 (sarcomere), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0099080 (supramolecular complex), GO:0099081 (supramolecular polymer), GO:0043292 (contractile fiber), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0099512 (supramolecular fiber), GO:0030016 (myofibril), GO:0043232 (intracellular non-membrane-bounded organelle)