Protein: Crossover junction endodeoxyribonuclease RuvC (EC 3.1.21.10) (Holliday junction nuclease RuvC) (Holliday junction resolvase RuvC) (P9WGV9)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair. Endonuclease that resolves HJ intermediates. Cleaves cruciform DNA by making single-stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction. The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange. HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.
Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00034}.
Sequence: MRVMGVDPGLTRCGLSLIESGRGRQLTALDVDVVRTPSDAALAQRLLAISDAVEHWLDTHHPEVVAIERVFSQLNVTTVMGTAQAGGVIALAAAKRGVDVHFHTPSEVKAAVTGNGSADKAQVTAMVTKILALQAKPTPADAADALALAICHCWRAPTIARMAEATSRAEARAAQQRHAYLAKLKAAR
InterPro domains: - IPR012337: Ribonuclease H-like superfamily (homologous_superfamily) [1-157]
- IPR020563: Crossover junction endodeoxyribonuclease RuvC, magnesium-binding site (binding_site) [114-149]
- IPR036397: Ribonuclease H superfamily (homologous_superfamily) [1-161]
- IPR002176: Crossover junction endodeoxyribonuclease RuvC (family) [1-163]
Interaction partners: - Holliday junction branch migration complex subunit RuvB
- Holliday junction branch migration complex subunit RuvA
- Protein RecA
- LexA repressor
- Regulatory protein RecX
- UvrABC system protein A
- Uncharacterized protein Rv2897c
- UvrABC system protein B
- Error-prone DNA polymerase
- ATP-dependent DNA helicase UvrD1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0140097 (catalytic activity, acting on DNA), GO:0004518 (nuclease activity), GO:0004536 (deoxyribonuclease activity), GO:0004520 (DNA endonuclease activity), GO:0004519 (endonuclease activity), GO:0016894 (endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters), GO:0016889 (DNA endonuclease activity, producing 3'-phosphomonoesters), GO:0008821 (crossover junction DNA endonuclease activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0006259 (DNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0006310 (DNA recombination), GO:0006281 (DNA repair), GO:0000725 (recombinational repair)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)