Protein: Ubiquitin conjugation factor E4 A (Q9U4F8)
Organism: Drosophila melanogaster (Fruit fly)
Function: Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates
Subcellular location: Cytoplasm
Sequence: MTSIEENPFAALLQEGANSDGVPHKLVEEVLLFTLNKASSSAVLCLADVVVETSEEMLSEDLVAHALFERLMLAETSQYAAGNASEMAMERRAMRYLKGAFTRCGLIQAERKIDCSKILALILNNASTCMRQPDLFAPQSFGAQWMEMFEQDEDEHDTSTQEFLIRVTCKVMEEVEPIEALGALKAIFYPVLTELQKNIAKLNLITMKKNTFWILGYFVRDKRAAVLGELLIDYTTPNPRASGSEYMDTLLGSLLCISILPKTQTGKYEFFQELSINQTDEALWALLSHHQQSIFLLVKQLLVLSPETKKKTLQWVGNCLDANVPRGHLWSSINASLEQTAHSTSSDAFMTNLTAVLVRLCAPLCMPSLKVLLVDPTYCAVPNKDRQAKGVSMLRAHAETCLLTSEEGEERLTAEKYNFVTEIFYMTHKCFELSNIPCIERFVRVLRELQNTQMAYGEIVNSDPNSEVAKNLFRMIRDQIQQVLTIKNTLAEPTNDMYLLKFFEASAIWLTEIAMLPREIYEQCLDKRDFSPQVFRNMELLSDTPPFVAPYMQSVPESIIDNISAFLNAARKLNGEQYINIYFSAHDAFFKMIILFMGSSALVKNPHLRAKLAEALEFLLPSRIMGSHRKTFVSHVFDNHPDRLKVVRSLLNVFVSIEMTGQSVQFEQKFNYRRPMYAIMEFLWTKPEHVQCFRDLAVEAEQNMDAIEPPIFLRFINLLINDAIFLLDESLSNLEQIKQLQQAQDNGEWESLPHTEREQHMTNLQHLGMLARFDNIIGRDTINLLKLLTSKIKSIFCHNSMVDRMAAMLNYFLLNLVGPKKERFKVKDKKEFEFDPAQTVIEISHIYINLSSDESFCLAVSQDGRSYSEQLFSYAENILIRIGGGQLIGDMSEFAVKVARMGAQYKEEQELLADAPEEYLDPIISTLMTDPVVLPSSKVTVDRSTIARHLLSDQTDPFNREPLTMDKVKSNEALKQEIESWIQGKREAARSNS
InterPro domains: - IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [914-992]
- IPR019474: Ubiquitin conjugation factor E4, core (domain) [251-898]
- IPR045132: Ubiquitin conjugation factor E4 (family) [22-990]
- IPR003613: U-box domain (domain) [914-988]
Interaction partners: - Nuclear protein localization protein 4 homolog
- LD25466p
- Transitional endoplasmic reticulum ATPase TER94
- Ubiquitin fusion degradation protein 1 homolog
- Ubiquitin thioesterase Otu1
- UV excision repair protein RAD23
- Ubiquilin-like protein
- UV excision repair protein RAD23
- Ubiquitin-conjugating enzyme E2-17 kDa
- FI07626p
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0019787 (ubiquitin-like protein transferase activity), GO:0004842 (ubiquitin-protein transferase activity), GO:0061659 (ubiquitin-like protein ligase activity), GO:0061630 (ubiquitin protein ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009605 (response to external stimulus), GO:0048584 (positive regulation of response to stimulus), GO:0009607 (response to biotic stimulus), GO:0002684 (positive regulation of immune system process), GO:0006807 (nitrogen compound metabolic process), GO:0048583 (regulation of response to stimulus), GO:0002682 (regulation of immune system process), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0051707 (response to other organism), GO:0044238 (primary metabolic process), GO:0002831 (regulation of response to biotic stimulus), GO:0032103 (positive regulation of response to external stimulus), GO:0050776 (regulation of immune response), GO:1901564 (organonitrogen compound metabolic process), GO:0043207 (response to external biotic stimulus), GO:0002833 (positive regulation of response to biotic stimulus), GO:0080134 (regulation of response to stress), GO:0009617 (response to bacterium), GO:0019538 (protein metabolic process), GO:0006952 (defense response), GO:0050778 (positive regulation of immune response), GO:0032101 (regulation of response to external stimulus), GO:0031349 (positive regulation of defense response), GO:0043170 (macromolecule metabolic process), GO:0098542 (defense response to other organism), GO:0036211 (protein modification process), GO:0031347 (regulation of defense response), GO:0045089 (positive regulation of innate immune response), GO:0043412 (macromolecule modification), GO:0045088 (regulation of innate immune response), GO:0042742 (defense response to bacterium), GO:0050829 (defense response to Gram-negative bacterium), GO:0070647 (protein modification by small protein conjugation or removal), GO:0032446 (protein modification by small protein conjugation), GO:0016567 (protein ubiquitination), GO:0000209 (protein polyubiquitination), GO:0070936 (protein K48-linked ubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)