Protein: PH and SEC7 domain-containing protein 1 (Exchange factor for ADP-ribosylation factor guanine nucleotide factor 6) (Exchange factor for ARF6) (Exchange factor for ARF6 A) (Pleckstrin homology and SEC7 domain-containing protein 1) (Q5DTT2)
Organism: Mus musculus (Mouse)
Function: Guanine nucleotide exchange factor for ARF6 (By similarity). Isoform 2 and isoform 3 induce cytoskeletal remodeling, but lead to distinct morphological changes in HeLa cells: isoform 2 induces cell elongation and formation of actin-rich protrusions, whereas isoform 3 promotes the formation of membrane ruffles and loss of stress fibers.
Subcellular location: [Isoform 1]: Cell membrane {ECO:0000269|PubMed:15540117, ECO:0000269|PubMed:17298598}. Cell projection, ruffle {ECO:0000269|PubMed:15540117, ECO:0000269|PubMed:17298598}. Note=Colocalizes with ACTN1 in membrane ruffles and central reticular structures. {ECO:0000269|PubMed:15540117, ECO:0000269|PubMed:17298598}.; SUBCELLULAR LOCATION: [Isoform 2]: Cell membrane {ECO:0000269|PubMed:19494129}. Cell projection {ECO:0000269|PubMed:19494129}. Note=Accumulates in microvilli-like protrusions. {ECO:0000269|PubMed:19494129}.; SUBCELLULAR LOCATION: [Isoform 3]: Cell membrane {ECO:0000269|PubMed:15540117, ECO:0000269|PubMed:19494129}. Cell projection, ruffle {ECO:0000269|PubMed:15540117, ECO:0000269|PubMed:19494129}. Note=Colocalizes with F-actin in membrane ruffles. {ECO:0000269|PubMed:15540117, ECO:0000269|PubMed:19494129}.; SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:A5PKW4}. Cell projection, ruffle membrane {ECO:0000250|UniProtKB:A5PKW4}. Cleavage furrow {ECO:0000250|UniProtKB:A5PKW4}. Note=Distributed uniformly on the plasma membrane, as well as throughout the cytoplasm during metaphase. Subsequently concentrated at patches in the equatorial region at the onset of cytokinesis, and becomes distributed in the equatorial region concurrent with cleavage furrow ingression. In later cytokinesis phases, fades away from the cleavage furrow and becomes uniformly distributed throughout the plasma membrane. {ECO:0000250|UniProtKB:A5PKW4}.
Sequence: MAQGAMRFCSEGDCAISPPRCPRRWLPEGPVPQSPPASMYGSTGSLIRRVVGPGPRGRDLGRVTAPCTPLRAPPSPHIAPSPWGPSSPTGQPPPGAQSSVVIFRFVEKASVRPLNGLPASGGLSRSWDLGGISAPRPTPALGPGCNRKLRLEASTSDPLPAGGGSVLPGSRDPSRGPLVPPQIGADGLYSSLPNGLGGTPEHLAMHFRGPADTGFLNQGDTWSSPREVSSHAQRIARAKWEFFYGSLDAPSSGAKPPEQVLPSRGVGSKQGSGVAVGRAAKYSETDLDKVPLRCYRETDIDEVLAEREEADSAIESQPSSEGPHGTAQPPASRPSPCPGPSSSLGSGNEDDEAGGEEDVDDEVFEASEGARPGDHMPHSGLLKSPVPFLLGTSPSADGPDSFSCVFEAILESHRAKGTSYSSLASLEALASPGPTQSPFFTFEMPPQPPAPRPDPPAPAPLAPLEPDSGTSSAADGPWTQRREVEESDAGATLAPRKELPSPSHSEDSFGLGAAPLGSEPPLSQLVSDSDSELDSTERLALGSTDTLSNGQKADLEAAQRLAKRLYRLDGFRKADVARHLGKNNDFSKLVAGEYLKFFVFTGMTLDQALRVFLKELALMGETQERERVLAHFSQRYFQCNPEALSSEDGAHTLTCALMLLNTDLHGHNIGKRMTCGDFIGNLEGLNDGGDFPRELLKALYSSIKNEKLQWAIDEEELRRSLSELADPNPKVIKRVSGGSGSSSSPFLDLTPEPGAAVYKHGALVRKVHADPDCRKTPRGKRGWKSFHGILKGMILYLQKEEYQPGKALSEAELKNAISIHHALATRASDYSKRPHVFYLRTADWRVFLFQAPSLEQMQSWITRINVVAAMFSAPPFPAAVSSQKKFSRPLLPSAATRLSQEEQVRTHEAKLKAMASELREHRAAHLGKKARGKEADEQRQKEAYLEFEKSRYGTYAALLRVKMKAASEELDTIEAALAQAGSTEDGCPPPHSSPSLRPKPTSQPRAQRPGSETRAGAGSTRPKP
InterPro domains: - IPR001849: Pleckstrin homology domain (domain) [756-871]
- IPR000904: Sec7 domain (domain) [519-708]
- IPR035999: Sec7 domain superfamily (homologous_superfamily) [542-712]
- IPR023394: Sec7, C-terminal domain superfamily (homologous_superfamily) [600-721]
- IPR001605: Pleckstrin homology domain, spectrin-type (domain) [781-862]
- IPR041681: Pleckstrin homology domain 9 (domain) [758-869]
- IPR011993: PH-like domain superfamily (homologous_superfamily) [753-872]
Interaction partners: - ADP-ribosylation factor 6
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0030234 (enzyme regulator activity), GO:0140677 (molecular function activator activity), GO:0140678 (molecular function inhibitor activity), GO:0060589 (nucleoside-triphosphatase regulator activity), GO:0005085 (guanyl-nucleotide exchange factor activity), GO:0030695 (GTPase regulator activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0065007 (biological regulation), GO:0051716 (cellular response to stimulus), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0050794 (regulation of cellular process), GO:0035556 (intracellular signal transduction), GO:0007264 (small GTPase mediated signal transduction), GO:0007265 (Ras protein signal transduction)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0031252 (cell leading edge), GO:0031256 (leading edge membrane), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0030054 (cell junction), GO:0098794 (postsynapse), GO:0044297 (cell body), GO:0032153 (cell division site), GO:0036477 (somatodendritic compartment), GO:0071944 (cell periphery), GO:0043025 (neuronal cell body), GO:0098590 (plasma membrane region), GO:0120025 (plasma membrane bounded cell projection), GO:0097447 (dendritic tree), GO:0032587 (ruffle membrane), GO:0045202 (synapse), GO:0032154 (cleavage furrow), GO:0001726 (ruffle), GO:0005886 (plasma membrane), GO:0043005 (neuron projection), GO:0031253 (cell projection membrane), GO:0098978 (glutamatergic synapse), GO:0030425 (dendrite)