Protein: Arginine ADP-riboxanase CopC (Q7NWF2)
Organism: Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / CCUG
Function: ADP-riboxanase effector that inhibits host cell programmed cell death. Acts by mediating arginine ADP-riboxanation of host caspases (CASP3, CASP7, CASP8 and CASP9), blocking their processing and activation. ADP- riboxanation of host apoptotic caspases (CASP3, CASP7, CASP8 and CASP9) prevents their activation, thereby inhibiting host cell apoptosis. ADP-riboxanation of host CASP8 also inhibits host cell necroptosis. ADP-riboxanation of host CASP3 also abolishes pyroptosis by preventing its ability to cleave GSDME. May also able to inactivate CASP4/CASP11, blocking inhibiting LPS- induced pyroptosis; however this activity is unsure in vivo. ADP-riboxanation takes place in several steps: CopC first binds host caspases and NAD(+); NAD(+) is hydrolyzed to nicotinamide and ADP-D-ribose. CopC then transfers the ADP-D-ribose to the modified arginine of caspases and forms the ADP-D-ribose-deacylization on arginine, leading to deamination to remove one N-omega group on target arginine
Subcellular location: Secreted. Host cytoplasm. Note=Secreted via the type III secretion system (T3SS)
Sequence: MRVENHSPSLSKLNPPEAGSGDPTAIGRRLSGIRRAPLPHVSAGSDGEAAAAGKIGAFLRKAVAAQSYGLMFANGKLFEATGDALEKRGQYGFSALQRLDGLSRRNLAAVEARLGALDSAERGLKERIMTGAWHFRHQSNAALDDGKTAAIASNHLLARESRSSGGNTFAGDKALLSNHDFVFFGVEFSGRGKQDKPLNHKHSTMDFGANAYVVPDTLPACRHGYLTLTDHFFNRVPGGREAEHQDFVGSFPQMGAETGRWIHEGKYRQNAPIFNYRDMKAAVALHLIEFLRDSKDAAFKAYVFDQAMQSGQALDRVLNSVFQAEFHIPRLMATTDYAKHPLRPMLLKEAVDSVNLPALSGLVSSKGDAVTAMWHAIDKGKDAVAAHLLGNWRFEAGDFASAPPGFYHELNYALSEHGASVYILDQFLSRGWAAVNAPFEHVNSGETMLDNAVKYGNREMAAALIKHGADRNLLSEWNGGKLDALLA
InterPro domains: - IPR010366: Arginine ADP-riboxanase OspC1-4 (family) [193-470]
- IPR036770: Ankyrin repeat-containing domain superfamily (homologous_superfamily) [343-479]
- IPR002110: Ankyrin repeat (repeat) [444-476]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016829 (lyase activity), GO:0016840 (carbon-nitrogen lyase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016841 (ammonia-lyase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009605 (response to external stimulus), GO:0044068 (modulation by symbiont of host cellular process), GO:0009607 (response to biotic stimulus), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0023051 (regulation of signaling), GO:0035821 (modulation of process of another organism), GO:0044403 (biological process involved in symbiotic interaction), GO:0051707 (response to other organism), GO:0052027 (modulation by symbiont of host signal transduction pathway), GO:0044501 (modulation of signal transduction in another organism), GO:0051701 (biological process involved in interaction with host), GO:0052040 (modulation by symbiont of host programmed cell death), GO:0043207 (response to external biotic stimulus), GO:0052173 (response to defenses of other organism), GO:0010646 (regulation of cell communication), GO:0044003 (modulation by symbiont of host process), GO:0009966 (regulation of signal transduction), GO:0075136 (response to host), GO:0052200 (response to host defenses), GO:0052041 (suppression by symbiont of host programmed cell death), GO:0052029 (suppression by symbiont of host signal transduction pathway), GO:0052031 (modulation by symbiont of host defense response), GO:1902531 (regulation of intracellular signal transduction), GO:0050848 (regulation of calcium-mediated signaling)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0018995 (host cellular component), GO:0033643 (host cell part), GO:0043657 (host cell), GO:0033646 (host intracellular part), GO:0043656 (host intracellular region), GO:0033647 (host intracellular organelle), GO:0030430 (host cell cytoplasm), GO:0033648 (host intracellular membrane-bounded organelle), GO:0042025 (host cell nucleus)