Protein: 2-hydroxymuconate tautomerase (EC 5.3.2.6) ((2Z,4E)-2-hydroxyhexa-2,4-dienedioate keto-enol isomerase) (4-oxalocrotonate tautomerase) (4-OT) (P70994)
Organism: Bacillus subtilis (strain 168)
Function: Catalyzes both 1,3- and 1,5-keto-enol tautomerization of the diacid 2-hydroxymuconate (2-hydroxy-2,4-hexadienedioate) to produce 2-oxo-4-hexenedioate. This reaction is highly stereoselective and produces a mixture of stereoisomers, where the (3S)-isomer of 2-oxo-4-hexenedioate predominates. Also catalyzes the tautomerization of 2-hydroxymuconate to 2-oxo-3-hexenedioate, however this reaction is slower and occurs after the tautomerization of 2-hydroxymuconate to 2-oxo-4-hexenedioate. Using 2-hydroxy-2,4-pentadienoate, phenylenolpyruvate, (p-hydroxyphenyl)-enolpyruvate and 2-hydroxy-2,4-heptadiene-1,7-dioate, YwhB is a highly efficient 1,3-keto-enol tautomerase, but clearly not a 1,5-keto-enol tautomerase. Tautomerization of the two monoacids 2-hydroxy-2,4-pentadienoate and phenylenolpyruvate produces a mixture of stereoisomers, where the (3R)-isomers predominate.
Sequence: MPYVTVKMLEGRTDEQKRNLVEKVTEAVKETTGASEEKIVVFIEEMRKDHYAVAGKRLSDME
InterPro domains: - IPR004370: 4-oxalocrotonate tautomerase-like domain (domain) [2-60]
- IPR018191: 4-oxalocrotonate tautomerase (family) [1-62]
- IPR014347: Tautomerase/MIF superfamily (homologous_superfamily) [1-61]
Interaction partners: - Catechol-2,3-dioxygenase
- 5'-nucleotidase YutF
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0009892 (negative regulation of metabolic process), GO:0019222 (regulation of metabolic process), GO:0048523 (negative regulation of cellular process), GO:0050794 (regulation of cellular process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051053 (negative regulation of DNA metabolic process), GO:0051052 (regulation of DNA metabolic process), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0006275 (regulation of DNA replication), GO:0008156 (negative regulation of DNA replication), GO:0090329 (regulation of DNA-templated DNA replication), GO:0030174 (regulation of DNA-templated DNA replication initiation), GO:2000104 (negative regulation of DNA-templated DNA replication)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0099080 (supramolecular complex), GO:0099081 (supramolecular polymer), GO:0043229 (intracellular organelle), GO:0043292 (contractile fiber), GO:0043228 (non-membrane-bounded organelle), GO:0030016 (myofibril), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0099512 (supramolecular fiber)