Protein: Sensor histidine kinase CheAY (EC 2.7.13.3) (O25153)
Organism: Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Function: Member of the two-component regulatory system CheAY/CheY that regulates chemotaxis and colonization of the gastric mucosa. Functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to the conserved aspartic acid residue in the regulatory domain of CheY. In turn, phosphorylated CheY (CheY-P) interacts with the flagellar motor protein FliM to cause clockwise flagellar rotation and bacterial reversals, as opposed to straight swimming when CheY is not phosphorylated (By similarity).
Sequence: MDDLQEIMEDFLIEAFEMNEQLDQDLVELEHNPEDLDLLNRIFRVAHTIKGSSSFLNLNILTHLTHNMEDVLNRARKGEIKITPDIMDVVLRSIDLMKTLLVTIRDTGSDTNNGKENEIEEAVKQLQAITSQNLESAKERTTEAPQKENKEETKEEAKEENKENKAKAPTAENTSSDNPLADEPDLDYANMSAEEVEAEIERLLNKRQEADKERRAQKKQEAKPKQEVTPTKETPKAPKTETKAKAKADTEENKAPSIGVEQTVRVDVRRLDHLMNLIGELVLGKNRLIRIYSDVEERYDGEKFLEELNQVVSSISAVTTDLQLAVMKTRMQPVGKVFNKFPRMVRDLSRELGKSIELIIEGEETELDKSIVEEIGDPLIHIIRNSCDHGIEPLEERRKLNKPETGKVQLSAYNEGNHIVIKISDDGKGLDPVMLKEKAIEKGVISERDAEGMSDREAFNLIFKPGFSTAKVVSNVSGRGVGMDVVKTNIEKLNGIIEIDSEVGVGTTQKLKIPLTLAIIQALLVGVQEEYYAIPLSSVLETVRISQDEIYTVDGKSVLRLRDEVLSLVRLSDIFKVDAILESNSDVYVVIIGLADQKIGVIVDYLIGQEEVVIKSLGYYLKNTRGIAGATVRGDGKITLIVDVGAMMDMAKSIKVNITTLMNESENTKSKNSPSDYIVLAIDDSSTDRAIIRKCLKPLGITLLEATNGLEGLEMLKNGDKIPDAILVDIEMPKMDGYTFASEVRKYNKFKNLPLIAVTSRVTKTDRMRGVESGMTEYITKPYSGEYLTTVVKRSIKLEGDQS
InterPro domains: - IPR036890: Histidine kinase/HSP90-like ATPase superfamily (homologous_superfamily) [330-516]
- IPR037006: Histidine kinase CheA-like, homodimeric domain superfamily (homologous_superfamily) [261-329]
- IPR036641: HPT domain superfamily (homologous_superfamily) [1-163]
- IPR002545: CheW-like domain (domain) [509-653]
- IPR008207: Signal transduction histidine kinase, phosphotransfer (Hpt) domain (domain) [1-104]
- IPR003594: Histidine kinase/HSP90-like ATPase domain (domain) [374-517]
- IPR001789: Signal transduction response regulator, receiver domain (domain) [677-796]
- IPR005467: Histidine kinase domain (domain) [270-517]
- IPR036061: CheW-like domain superfamily (homologous_superfamily) [516-647]
- IPR036097: Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily (homologous_superfamily) [262-330]
- IPR011006: CheY-like superfamily (homologous_superfamily) [676-797]
- IPR004105: Histidine kinase CheA-like, homodimeric domain (domain) [261-329]
- IPR004358: Signal transduction histidine kinase-related protein, C-terminal (domain) [419-514]
- IPR051315: Bacterial Chemotaxis Sensor Histidine Kinase CheA (family) [3-792]
Interaction partners: - Methyl-accepting chemotaxis transducer
- Chemotaxis protein CheW
- Chemotaxis protein CheV1
- Methyl-accepting chemotaxis protein
- Methyl-accepting chemotaxis protein
- Chemotaxis protein CheV2
- Chemotaxis protein CheV3
- Hemolysin secretion protein
- Flagellar motor switch protein FliN
- Chemotaxis protein CheY1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0065009 (regulation of molecular function), GO:0050790 (regulation of catalytic activity), GO:0044093 (positive regulation of molecular function), GO:0043085 (positive regulation of catalytic activity), GO:0051339 (regulation of lyase activity), GO:0031279 (regulation of cyclase activity), GO:0045761 (regulation of adenylate cyclase activity), GO:0031281 (positive regulation of cyclase activity), GO:0051349 (positive regulation of lyase activity), GO:0045762 (positive regulation of adenylate cyclase activity)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005829 (cytosol), GO:1902494 (catalytic complex), GO:0005622 (intracellular anatomical structure), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0140535 (intracellular protein-containing complex), GO:0005886 (plasma membrane)