Protein: DNA polymerase epsilon subunit B (DNA polymerase II subunit 2) (P24482)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: As accessory component of the DNA polymerase epsilon complex participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus {ECO:0000269|PubMed:14562095}.
Sequence: MFGSGNVLPVKIQPPLLRPLAYRVLSRKYGLSIKSDGLSALAEFVGTNIGANWRQGPATIKFLEQFAAVWKQQERGLFIDQSGVKEVIQEMKEREKVEWSHEHPIQHEENILGRTDDDENNSDDEMPIAADSSLQNVSLSSPMRQPTERDEYKQPFKPESSKALDWRDYFKVINASQQQRFSYNPHKMQFIFVPNKKQNGLGGIAGFLPDIEDKVQMFLTRYYLTNDRVMRNENFQNSDMFNPLSSMVSLQNELSNTNRQQQSSSMSITPIKNLLGRDAQNFLLLGLLNKNFKGNWSLEDPSGSVEIDISQTIPTQGHYYVPGCMVLVEGIYYSVGNKFHVTSMTLPPGERREITLETIGNLDLLGIHGISNNNFIARLDKDLKIRLHLLEKELTDHKFVILGANLFLDDLKIMTALSKILQKLNDDPPTLLIWQGSFTSVPVFASMSSRNISSSTQFKNNFDALATLLSRFDNLTENTTMIFIPGPNDLWGSMVSLGASGTLPQDPIPSAFTKKINKVCKNVVWSSNPTRIAYLSQEIVIFRDDLSGRFKRHRLEFPFNESEDVYTENDNMMSKDTDIVPIDELVKEPDQLPQKVQETRKLVKTILDQGHLSPFLDSLRPISWDLDHTLTLCPIPSTMVLCDTTSAQFDLTYNGCKVINPGSFIHNRRARYMEYVPSSKKTIQEEIYI
InterPro domains: - IPR007185: DNA polymerase alpha/delta/epsilon, subunit B (domain) [400-650]
- IPR016266: DNA polymerase epsilon, subunit B (family) [15-687]
Interaction partners: - DNA replication complex GINS protein SLD5
- DNA polymerase epsilon subunit C
- DNA polymerase epsilon catalytic subunit A
- DNA replication complex GINS protein PSF3
- DNA replication complex GINS protein PSF1
- DNA replication licensing factor MCM3
- Minichromosome maintenance protein 5
- Cell division control protein 45
- DNA polymerase epsilon subunit D
- DNA replication complex GINS protein PSF2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:1901363 (heterocyclic compound binding), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0140097 (catalytic activity, acting on DNA), GO:0016779 (nucleotidyltransferase activity), GO:0003677 (DNA binding), GO:0034061 (DNA polymerase activity), GO:0003690 (double-stranded DNA binding), GO:0043565 (sequence-specific DNA binding), GO:0003697 (single-stranded DNA binding), GO:0003887 (DNA-directed DNA polymerase activity), GO:1990837 (sequence-specific double-stranded DNA binding), GO:0003688 (DNA replication origin binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0044786 (cell cycle DNA replication), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0033260 (nuclear DNA replication), GO:0006259 (DNA metabolic process), GO:0006996 (organelle organization), GO:0022616 (DNA strand elongation), GO:0006260 (DNA replication), GO:0006270 (DNA replication initiation), GO:0000723 (telomere maintenance), GO:0051276 (chromosome organization), GO:0032200 (telomere organization), GO:0006261 (DNA-templated DNA replication), GO:0016233 (telomere capping), GO:0006271 (DNA strand elongation involved in DNA replication), GO:0006273 (lagging strand elongation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0032993 (protein-DNA complex), GO:0140513 (nuclear protein-containing complex), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0031261 (DNA replication preinitiation complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005694 (chromosome), GO:0005634 (nucleus), GO:0000228 (nuclear chromosome)