Protein: Malate synthase 1 (EC 2.3.3.9) (P30952)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Malate synthase which takes part in the glyoxylate cycle. MLS1 activity is essential for cells to grow on oleic acid as a sole carbon source. Two steps of the glyoxylate cycle take place in the cytosol, the splitting of isocitrate into succinate and glyoxylate, and the dehydrogenation of malate to oxaloacetate. However, the formation of malate from glyoxylate and acetyl-CoA undertaken MLS1, occurs in the peroxisomes when cells are grown on oleic acid (Probable). The source of acetyl-CoA being either peroxisomal when breaking down fatty acids, or cytosolic when extra-cellular two-carbon substrates are used, therefore, although not strictly essential, the peroxisomal localization of MLS1 appears to be advantageous for cells growing on oleic acid, in that acetyl-CoA production and utilization are thereby intimately compartmentalized together to increase efficiency (Probable).
Subcellular location: Peroxisome matrix {ECO:0000269|PubMed:11846793, ECO:0000269|PubMed:27678487}. Note=Imported in peroxisome via recognition by the peroxisomal targeting signal receptor PEX9 in an oleate-dependent manner. {ECO:0000269|PubMed:27678487}.
Sequence: MVKVSLDNVKLLVDVDKEPFFKPSSTTVGDILTKDALEFIVLLHRTFNNKRKQLLENRQVVQKKLDSGSYHLDFLPETANIRNDPTWQGPILAPGLINRSTEITGPPLRNMLINALNAPVNTYMTDFEDSASPTWNNMVYGQVNLYDAIRNQIDFDTPRKSYKLNGNVANLPTIIVRPRGWHMVEKHLYVDDEPISASIFDFGLYFYHNAKELIKLGKGPYFYLPKMEHHLEAKLWNDVFCVAQDYIGIPRGTIRATVLIETLPAAFQMEEIIYQLRQHSSGLNCGRWDYIFSTIKRLRNDPNHILPNRNQVTMTSPFMDAYVKRLINTCHRRGVHAMGGMAAQIPIKDDPAANEKAMTKVRNDKIRELTNGHDGSWVAHPALAPICNEVFINMGTPNQIYFIPENVVTAANLLETKIPNGEITTEGIVQNLDIGLQYMEAWLRGSGCVPINNLMEDAATAEVSRCQLYQWVKHGVTLKDTGEKVTPELTEKILKEQVERLSKASPLGDKNKFALAAKYFLPEIRGEKFSEFLTTLLYDEIVSTKATPTDLSKL
InterPro domains: - IPR048355: Malate synthase, C-terminal domain (domain) [422-541]
- IPR046363: Malate synthase, N-terminal and TIM-barrel domains (homologous_superfamily) [17-421]
- IPR019830: Malate synthase, conserved site (conserved_site) [277-292]
- IPR011076: Malate synthase superfamily (homologous_superfamily) [27-543]
- IPR006252: Malate synthase A (family) [11-546]
- IPR001465: Malate synthase, TIM barrel domain (domain) [173-415]
- IPR044856: Malate synthase, C-terminal superfamily (homologous_superfamily) [422-542]
- IPR048356: Malate synthase, N-terminal domain (domain) [17-79]
Interaction partners: - Mitochondrial 2-methylisocitrate lyase ICL2
- Isocitrate lyase
- Acetyl-coenzyme A synthetase 1
- Malate dehydrogenase, cytoplasmic
- Citrate synthase, peroxisomal
- Phosphoenolpyruvate carboxykinase
- Citrate synthase 3, mitochondrial
- Citrate synthase, mitochondrial
- Acetyl-coenzyme A synthetase 2
- Malate dehydrogenase, peroxisomal
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016746 (acyltransferase activity), GO:0046912 (acyltransferase activity, acyl groups converted into alkyl on transfer)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0006807 (nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0043603 (amide metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0046483 (heterocycle metabolic process), GO:0043605 (amide catabolic process), GO:0044248 (cellular catabolic process), GO:0046700 (heterocycle catabolic process), GO:0000255 (allantoin metabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:1901361 (organic cyclic compound catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0042579 (microbody), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005777 (peroxisome)