Protein: Elongator complex protein 3 (Q1ZXC6)
Organism: Dictyostelium discoideum (Social amoeba)
Function: Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5- methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (By similarity). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity)
Sequence: MSLMNDPRPVSKKAGAKPVYGKNTPQFTKTVGEIVNALINAYKEGKKVNLLKIKTELAAKNSLSDQPKSVDIISAIPESYKNTLLPLLKAKPVRTASGIAVVAVMCKPHRCPHLAMTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYNPFLQTRHRIDQLKRLGHNVEKVEFIIMGGTFMSLPSDYRDYFIRNLHDALSGHTSNNVAEAVKYSEQSNVKCVGITIETRPDHCLKLHLSNMLTYGCTRLEIGVQSVFEDIARDTNRGHTVRAVLESFQLAKDSGFKVVAHMMPDLPNMGMERDIYGFMEFFENPAFRADGLKIYPTLVIRGTGLYELWKTGTYKNYSPDSLVDLIAKVLALVPPWTRIYRIQRDIPMPLVTSGVEYGNLRELCLARMKDFGTKCRDVRTREVGIQEVHHKIKPDQVELIRRDYVANGGWETFLSFEDPKQDILIGLLRLRKCSETSFRPELKENCSIVRELHVYGSVVGIHNRDPTKFQHQGYGTLLMEEAERIAREEHGSIKLAVIAGVGTRHYYRKLGYELDGVYVSKYLG
InterPro domains: - IPR000182: GNAT domain (domain) [408-559]
- IPR058240: Radical SAM superfamily (homologous_superfamily) [131-370]
- IPR056591: ELP3-like, N-terminal domain (domain) [27-96]
- IPR007197: Radical SAM (domain) [47-558]
- IPR032432: Radical SAM, C-terminal extension (domain) [325-404]
- IPR034687: Elongator complex protein 3-like (family) [24-558]
- IPR039661: ELP3/YhcC (family) [21-558]
- IPR016181: Acyl-CoA N-acyltransferase (homologous_superfamily) [367-554]
- IPR006638: Elp3/MiaA/NifB-like, radical SAM core domain (domain) [101-363]
Interaction partners: - Elongator complex protein 1
- Elongator complex protein 4
- Elongator complex protein 2
- Cytoplasmic tRNA 2-thiolation protein 2
- Protein KTI12 homolog
- Cytoplasmic tRNA 2-thiolation protein 1
- Elongator complex protein 6
- Diphthamide biosynthesis protein 3
- DNA replication licensing factor mcm5
- Adenylyltransferase and sulfurtransferase MOCS3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016746 (acyltransferase activity), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups), GO:0016407 (acetyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0009451 (RNA modification), GO:0006400 (tRNA modification), GO:0034470 (ncRNA processing), GO:0034660 (ncRNA metabolic process), GO:0002097 (tRNA wobble base modification), GO:0006399 (tRNA metabolic process), GO:0008033 (tRNA processing), GO:0002098 (tRNA wobble uridine modification)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0140535 (intracellular protein-containing complex), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0005798 (Golgi-associated vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0097708 (intracellular vesicle)