Protein: Cytochrome b5 reductase 4 (EC 1.6.2.2) (Flavohemoprotein b5/b5R) (b5+b5R) (N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein) (cb5/cb5R) (Q68EJ0)
Organism: Rattus norvegicus (Rat)
Function: NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS).
Subcellular location: Endoplasmic reticulum. Note=Soluble protein. {ECO:0000250}.
Sequence: MLNVPSQAFPAPGSQQRVASQGRSKVPLKQGRSLMDWFRLTKSGKDFTGLKGGLIEVTEEELKKHNKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKDCHEGKRVLNGMLPKSQVTDTLPREGPSSPSYDWFQTESSVTIVIYTKQKNINLDSVIVDLQDDSLRAEAVIKDHSYLIHIGLSHEVQENFSVRVIENVGKIEIVLQKKETVSWKCLGDPLEKHDSFIPKKDTGLYYRQCQLISKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGAEIVKPYTPVSESLLSDFKEPVLSPNKYIYFLIKIYPAGLFTPELDRLQIGDFVSVSGPEGNFKVSKLQEVEDLFLLAAGTGFTPMVTVLNHALTHMSSLRKVKLMFFNKTEDDIIWRCQLEKLALKDKRFHVEYVLSAPSPEWNGKQGHVSRALLSEFLQRSLENSKVFLCICGPTPFTDEGIRLLHDLNFSDDEIHGFTA
InterPro domains: - IPR051872: Cytochrome b5/Flavoprotein Reductase (family) [16-129]
- IPR017938: Riboflavin synthase-like beta-barrel (homologous_superfamily) [266-383]
- IPR007052: CS domain (domain) [164-255]
- IPR001433: Oxidoreductase FAD/NAD(P)-binding (domain) [394-498]
- IPR018506: Cytochrome b5, heme-binding site (binding_site) [85-92]
- IPR036400: Cytochrome b5-like heme/steroid binding domain superfamily (homologous_superfamily) [51-136]
- IPR017927: FAD-binding domain, ferredoxin reductase-type (domain) [272-384]
- IPR008333: Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain (domain) [277-382]
- IPR008978: HSP20-like chaperone (homologous_superfamily) [161-268]
- IPR039261: Ferredoxin-NADP reductase (FNR), nucleotide-binding domain (homologous_superfamily) [370-518]
- IPR001199: Cytochrome b5-like heme/steroid binding domain (domain) [54-130]
- IPR037908: Cytochrome b5 reductase 4, p23 domain (domain) [169-255]
Interaction partners: - Dihydroorotate dehydrogenase
- Dihydropyrimidine dehydrogenase [NADP
- Uridine monophosphate synthetase, isoform CRA_a.
- Cytidine monophospho-N-acetylneuraminic acid hydroxylase, pseudogene
- NADPH:adrenodoxin oxidoreductase, mitochondrial
- Cytochrome b5
- Cytochrome b5 type B
- Sulfite oxidase, mitochondrial
- Cytochrome b5 domain-containing protein 1
- Fatty acid desaturase 6
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016651 (oxidoreductase activity, acting on NAD(P)H), GO:0016653 (oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0072593 (reactive oxygen species metabolic process), GO:0006801 (superoxide metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0044297 (cell body), GO:0098793 (presynapse), GO:0036477 (somatodendritic compartment), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0030054 (cell junction), GO:0043025 (neuronal cell body), GO:0008021 (synaptic vesicle), GO:0120025 (plasma membrane bounded cell projection), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0045202 (synapse), GO:0043227 (membrane-bounded organelle), GO:0030133 (transport vesicle), GO:0070382 (exocytic vesicle), GO:0043005 (neuron projection), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0030424 (axon), GO:0097708 (intracellular vesicle)