Protein: Proteasome subunit beta type-3 (Macropain subunit PUP3) (Multicatalytic endopeptidase complex subunit PUP3) (Proteasome component PUP3) (P25451)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Non-catalytic component of the proteasome which degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity. This subunit may participate in the trypsin-like activity of the enzyme complex.
Subcellular location: Cytoplasm. Nucleus.
Sequence: MSDPSSINGGIVVAMTGKDCVAIACDLRLGSQSLGVSNKFEKIFHYGHVFLGITGLATDVTTLNEMFRYKTNLYKLKEERAIEPETFTQLVSSSLYERRFGPYFVGPVVAGINSKSGKPFIAGFDLIGCIDEAKDFIVSGTASDQLFGMCESLYEPNLEPEDLFETISQALLNAADRDALSGWGAVVYIIKKDEVVKRYLKMRQD
InterPro domains: - IPR023333: Proteasome B-type subunit (family) [3-198]
- IPR029055: Nucleophile aminohydrolases, N-terminal (homologous_superfamily) [2-205]
- IPR001353: Proteasome, subunit alpha/beta (family) [8-190]
- IPR016050: Proteasome beta-type subunit, conserved site (conserved_site) [13-59]
- IPR033811: Proteasome beta 3 subunit (family) [8-201]
Interaction partners: - 26S proteasome regulatory subunit RPN8
- 26S proteasome subunit RPT4
- Proteasome subunit alpha type-4
- Proteasome maturation factor UMP1
- 26S proteasome regulatory subunit RPN5
- 26S proteasome regulatory subunit RPN12
- Proteasome subunit beta type-6
- Proteasome subunit alpha type-3
- Proteasome subunit alpha type-5
- 26S proteasome regulatory subunit 4 homolog
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044248 (cellular catabolic process), GO:0019538 (protein metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009057 (macromolecule catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0044265 (cellular macromolecule catabolic process), GO:0030163 (protein catabolic process), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0010498 (proteasomal protein catabolic process), GO:0051603 (proteolysis involved in protein catabolic process), GO:0010499 (proteasomal ubiquitin-independent protein catabolic process), GO:0019941 (modification-dependent protein catabolic process), GO:0043161 (proteasome-mediated ubiquitin-dependent protein catabolic process), GO:0006511 (ubiquitin-dependent protein catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0140535 (intracellular protein-containing complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0031984 (organelle subcompartment), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0019774 (proteasome core complex, beta-subunit complex), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0031090 (organelle membrane), GO:0000502 (proteasome complex), GO:0005783 (endoplasmic reticulum), GO:0005839 (proteasome core complex), GO:1905368 (peptidase complex), GO:0034515 (proteasome storage granule), GO:0043229 (intracellular organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005789 (endoplasmic reticulum membrane), GO:0043232 (intracellular non-membrane-bounded organelle), GO:1905369 (endopeptidase complex), GO:0005634 (nucleus)