Protein: Transcriptional regulator URE2 (Disulfide reductase) (EC 1.8.4.-) (Glutathione peroxidase) (EC 1.11.1.9) (P23202)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Plays an important role in nitrogen catabolite repression. Down-regulates the expression of many genes involved in nitrogen utilization by inhibiting the GATA transcriptional activators GLN3 and GAT1. Under good nitrogen conditions, binds to the phosphorylated forms of GLN3 and GAT1 and sequesters them in the cytoplasm, preventing transcription of genes expressed upon nitrogen limitation. Is also an atypical glutaredoxin without a catalytical cysteine residue. Has glutathione peroxidase and thiol:disulfide oxidoreductase activities in both native and fibrillar form. Also shows insulin disulfide reductase and dehydroascorbic acid reductase (DHAR) activities.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:10604478}.
Sequence: MMNNNGNQVSNLSNALRQVNIGNRNSNTTTDQSNINFEFSTGVNNNNNNNSSSNNNNVQNNNSGRNGSQNNDNENNIKNTLEQHRQQQQAFSDMSHVEYSRITKFFQEQPLEGYTLFSHRSAPNGFKVAIVLSELGFHYNTIFLDFNLGEHRAPEFVSVNPNARVPALIDHGMDNLSIWESGAILLHLVNKYYKETGNPLLWSDDLADQSQINAWLFFQTSGHAPMIGQALHFRYFHSQKIASAVERYTDEVRRVYGVVEMALAERREALVMELDTENAAAYSAGTTPMSQSRFFDYPVWLVGDKLTIADLAFVPWNNVVDRIGINIKIEFPEVYKWTKHMMRRPAVIKALRGE
InterPro domains: - IPR036249: Thioredoxin-like superfamily (homologous_superfamily) [103-195]
- IPR036282: Glutathione S-transferase, C-terminal domain superfamily (homologous_superfamily) [201-351]
- IPR017298: Transcriptional regulator Ure2 (family) [1-354]
- IPR004046: Glutathione S-transferase, C-terminal (domain) [224-345]
- IPR010987: Glutathione S-transferase, C-terminal-like (domain) [205-354]
- IPR040079: Glutathione transferase family (family) [114-344]
- IPR004045: Glutathione S-transferase, N-terminal (domain) [112-196]
Interaction partners: - Nitrogen regulatory protein GLN3
- Eukaryotic peptide chain release factor GTP-binding subunit
- [PIN+] prion protein RNQ1
- Mitochondrial protein import protein MAS5
- Glutathione reductase
- Glutathione hydrolase proenzyme
- Transcriptional regulatory protein GAT1
- Nitrogen regulatory protein DAL80
- Peroxiredoxin PRX1, mitochondrial
- Heat shock protein 104
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity), GO:0005515 (protein binding), GO:0140318 (protein transporter activity), GO:0008320 (protein transmembrane transporter activity), GO:0022884 (macromolecule transmembrane transporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0055085 (transmembrane transport), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0033036 (macromolecule localization), GO:0044085 (cellular component biogenesis), GO:0046907 (intracellular transport), GO:0071806 (protein transmembrane transport), GO:0006810 (transport), GO:1990542 (mitochondrial transmembrane transport), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0051668 (localization within membrane), GO:0045184 (establishment of protein localization), GO:0006839 (mitochondrial transport), GO:0090151 (establishment of protein localization to mitochondrial membrane), GO:0006886 (intracellular protein transport), GO:0071705 (nitrogen compound transport), GO:0015031 (protein transport), GO:0072594 (establishment of protein localization to organelle), GO:0071702 (organic substance transport), GO:0065002 (intracellular protein transmembrane transport), GO:0030150 (protein import into mitochondrial matrix), GO:0043933 (protein-containing complex organization), GO:0008104 (protein localization), GO:0044743 (protein transmembrane import into intracellular organelle), GO:0006996 (organelle organization), GO:0090150 (establishment of protein localization to membrane), GO:0072657 (protein localization to membrane), GO:0022607 (cellular component assembly), GO:0072655 (establishment of protein localization to mitochondrion), GO:0007005 (mitochondrion organization), GO:0033365 (protein localization to organelle), GO:0006605 (protein targeting), GO:0051205 (protein insertion into membrane), GO:0065003 (protein-containing complex assembly), GO:0051204 (protein insertion into mitochondrial membrane), GO:0006626 (protein targeting to mitochondrion), GO:0070585 (protein localization to mitochondrion), GO:0045040 (protein insertion into mitochondrial outer membrane), GO:0007008 (outer mitochondrial membrane organization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)