Protein: Embryonic polarity protein dorsal (P15330)
Organism: Drosophila melanogaster (Fruit fly)
Function: Embryonic developmental protein. The lateral or ventral identity of a cell depends upon the concentration of this protein in its nucleus during the blastoderm stage. A morphogenetic protein that specifically binds to the kappa B-related consensus sequence 5'-GRGAAAANCC-3', located in the enhancer region of zygotic genes such as Zen, Twist, Snail and Decapentaplegic. Mediates an immune response in larvae. Part of a signaling pathway involving NF-kappa-B and Toll-related receptors, that functions in the apoptosis of unfit cells during cell competition. May be part of a NF-kappa-B and Tollo signaling cascade that regulates development of the peripheral nervous system.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:14638854, ECO:0000269|PubMed:17032737, ECO:0000269|PubMed:2598266}. Nucleus {ECO:0000269|PubMed:14638854, ECO:0000269|PubMed:17032737, ECO:0000269|PubMed:2598266}. Note=In ventral regions it is first cytoplasmic, then the protein is relocalized in the nucleus (PubMed:2598266). Its nuclear localization is essential to its function as a morphogen (PubMed:2598266). In dorsal regions it remains cytoplasmic (PubMed:2598266). Tamo negatively regulates nuclear import of dl (PubMed:12653959). Emb is responsible for export of dl from the nucleus (PubMed:14638854). Nuclear localization is enhanced upon microbial infection (PubMed:17032737). {ECO:0000269|PubMed:12653959, ECO:0000269|PubMed:14638854, ECO:0000269|PubMed:17032737, ECO:0000269|PubMed:2598266}.; SUBCELLULAR LOCATION: [Isoform A]: Nucleus {ECO:0000305}.
Sequence: MFPNQNNGAAPGQGPAVDGQQSLNYNGLPAQQQQQLAQSTKNVRKKPYVKITEQPAGKALRFRYECEGRSAGSIPGVNSTPENKTYPTIEIVGYKGRAVVVVSCVTKDTPYRPHPHNLVGKEGCKKGVCTLEINSETMRAVFSNLGIQCVKKKDIEAALKAREEIRVDPFKTGFSHRFQPSSIDLNSVRLCFQVFMESEQKGRFTSPLPPVVSEPIFDKKAMSDLVICRLCSCSATVFGNTQIILLCEKVAKEDISVRFFEEKNGQSVWEAFGDFQHTDVHKQTAITFKTPRYHTLDITEPAKVFIQLRRPSDGVTSEALPFEYVPMDSGKHTFWNLHRHLKRKPDEDLFQQILRLDAKREVQPPTIEVIDLDTPKIDVQREIPSEMEFNHEESQQSEPALEQEQSVQQEQYTQEQSLQQEQYTQEQSLQQEQYLQQLEQQQSFQLEEPMQQDQELPAQQSFDQAIDHLPDHTSDHIPEDMEAADAHAEAEAHRLRSEQEKEIDTIIDEKVRELEQLDLGQQLEPRPLTANDKITEWMKSSEIEQQVHEPSPTAEADVLDSALEISKADKTLDELLETVAELDEIYTDFKVQRDTYKNTIQNELAGLQGRAPLQVEDSFDDAATYTSLQIAFKNPVLIPMDDIMPPTPPMSQCAPEDAHQHYDPVEVNSQARKPETPMRPVPPVPPAILTIQYPPEEDKLPPLPPKRIRKQDSNAENRSIEANTVQTKPSTGESPLNKRLPPAPKNPNFNTLPRQKKPGFFSKLFSRRKSKPDLAQGQENSSILDSKANSREPSIGHFNMQDPMRASLRSSKSAAPFISNPAPAKSSPVKAKKPGSKLTKPVGRSVSSVSGKRPAYLNADVVHIPLKGDSVNSLPQQQRTEGYSQSSTISVGAGLDRRTASALQLADIPISEGGMELVAIADRQSLHNLVSSIEGHFNVQLDPNLDLTEAEHFALYTSIPPLAAASEFDETSAYYAPVDAGEILTPDEVAKRLAAANGI
InterPro domains: - IPR002909: IPT domain (domain) [224-325]
- IPR000451: NF-kappa-B/Dorsal (family) [31-957]
- IPR037059: Rel homology domain (RHD), DNA-binding domain superfamily (homologous_superfamily) [46-220]
- IPR033926: NFkappaB IPT domain (domain) [225-326]
- IPR032397: Rel homology dimerisation domain (domain) [227-327]
- IPR008967: p53-like transcription factor, DNA-binding domain superfamily (homologous_superfamily) [46-228]
- IPR013783: Immunoglobulin-like fold (homologous_superfamily) [222-339]
- IPR014756: Immunoglobulin E-set (homologous_superfamily) [222-339]
- IPR011539: Rel homology domain, DNA-binding domain (domain) [44-223]
- IPR030492: Rel homology domain, conserved site (conserved_site) [62-68]
Interaction partners: - NF-kappa-B inhibitor cactus
- Dorsal-related immunity factor Dif
- Nuclear factor NF-kappa-B p110 subunit
- Daisho1
- Cecropin-B
- Drosomycin-like 3
- Dro6 protein
- Dro1 protein
- Cecropin-C
- Dro2 protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:0003682 (chromatin binding), GO:0097159 (organic cyclic compound binding), GO:0003700 (DNA-binding transcription factor activity), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003677 (DNA binding), GO:0043565 (sequence-specific DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0002376 (immune system process), GO:0023052 (signaling), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0023057 (negative regulation of signaling), GO:0009653 (anatomical structure morphogenesis), GO:0048584 (positive regulation of response to stimulus), GO:0019222 (regulation of metabolic process), GO:0006955 (immune response), GO:0002684 (positive regulation of immune system process), GO:0002682 (regulation of immune system process), GO:0051239 (regulation of multicellular organismal process), GO:0009892 (negative regulation of metabolic process), GO:0050793 (regulation of developmental process), GO:0048522 (positive regulation of cellular process), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0009893 (positive regulation of metabolic process), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0048523 (negative regulation of cellular process), GO:0048585 (negative regulation of response to stimulus), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0050777 (negative regulation of immune response), GO:0009968 (negative regulation of signal transduction), GO:0010648 (negative regulation of cell communication), GO:0032103 (positive regulation of response to external stimulus), GO:0048729 (tissue morphogenesis), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0080134 (regulation of response to stress), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0009966 (regulation of signal transduction), GO:0060255 (regulation of macromolecule metabolic process), GO:0032101 (regulation of response to external stimulus), GO:0002700 (regulation of production of molecular mediator of immune response), GO:0035070 (salivary gland histolysis), GO:0045087 (innate immune response), GO:0009891 (positive regulation of biosynthetic process), GO:0008356 (asymmetric cell division), GO:0035295 (tube development), GO:0000902 (cell morphogenesis), GO:0002831 (regulation of response to biotic stimulus), GO:0009887 (animal organ morphogenesis), GO:0050776 (regulation of immune response), GO:0042127 (regulation of cell population proliferation), GO:0009888 (tissue development), GO:0010646 (regulation of cell communication), GO:0031325 (positive regulation of cellular metabolic process), GO:0002702 (positive regulation of production of molecular mediator of immune response), GO:0002697 (regulation of immune effector process), GO:0002833 (positive regulation of response to biotic stimulus), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0006959 (humoral immune response), GO:0009889 (regulation of biosynthetic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0050778 (positive regulation of immune response), GO:0045595 (regulation of cell differentiation), GO:0009790 (embryo development), GO:0007166 (cell surface receptor signaling pathway), GO:0031323 (regulation of cellular metabolic process), GO:0031349 (positive regulation of defense response), GO:0002699 (positive regulation of immune effector process), GO:0080090 (regulation of primary metabolic process), GO:0008284 (positive regulation of cell population proliferation), GO:0035239 (tube morphogenesis), GO:0031347 (regulation of defense response), GO:0002784 (regulation of antimicrobial peptide production), GO:0034248 (regulation of amide metabolic process), GO:0002759 (regulation of antimicrobial humoral response), GO:0034250 (positive regulation of amide metabolic process), GO:1900426 (positive regulation of defense response to bacterium), GO:0002922 (positive regulation of humoral immune response), GO:0002922 (positive regulation of humoral immune response), GO:0002760 (positive regulation of antimicrobial humoral response), GO:0010628 (positive regulation of gene expression), GO:0002009 (morphogenesis of an epithelium), GO:0002225 (positive regulation of antimicrobial peptide production), GO:1900424 (regulation of defense response to bacterium), GO:0010468 (regulation of gene expression), GO:0048666 (neuron development), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0022008 (neurogenesis), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0000904 (cell morphogenesis involved in differentiation), GO:0034248 (regulation of amide metabolic process), GO:0002759 (regulation of antimicrobial humoral response), GO:0007399 (nervous system development), GO:0002759 (regulation of antimicrobial humoral response), GO:0007422 (peripheral nervous system development), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0048813 (dendrite morphogenesis), GO:0007390 (germ-band shortening), GO:0072002 (Malpighian tubule development), GO:0002920 (regulation of humoral immune response), GO:0060429 (epithelium development), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0030182 (neuron differentiation), GO:0010629 (negative regulation of gene expression), GO:0009792 (embryo development ending in birth or egg hatching), GO:0048667 (cell morphogenesis involved in neuron differentiation), GO:0002805 (regulation of antimicrobial peptide biosynthetic process), GO:0002786 (regulation of antibacterial peptide production), GO:0003383 (apical constriction), GO:0046530 (photoreceptor cell differentiation), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0033209 (tumor necrosis factor-mediated signaling pathway), GO:0006364 (rRNA processing), GO:0010468 (regulation of gene expression), GO:0042461 (photoreceptor cell development), GO:0010468 (regulation of gene expression), GO:0042461 (photoreceptor cell development), GO:0022416 (chaeta development), GO:2000112 (regulation of cellular macromolecule biosynthetic process), GO:1902680 (positive regulation of RNA biosynthetic process), GO:0048699 (generation of neurons), GO:0007391 (dorsal closure), GO:0002807 (positive regulation of antimicrobial peptide biosynthetic process), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045893 (positive regulation of DNA-templated transcription), GO:0002808 (regulation of antibacterial peptide biosynthetic process), GO:0001745 (compound eye morphogenesis), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0042462 (eye photoreceptor cell development), GO:0045944 (positive regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)