Protein: Serine/threonine-protein kinase Nek6 (P59895)
Organism: Rattus norvegicus (Rat)
Function: Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase- anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence (By similarity). Phosphorylates RPS6KB1. Phosphorylates EML4 at 'Ser-144', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (By similarity)
Subcellular location: Cytoplasm. Nucleus. Nucleus speckle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Colocalizes with APBB1 at the nuclear speckles. Colocalizes with PIN1 in the nucleus. Colocalizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis (By similarity)
Sequence: MAGQPSHMPHGGSPNHLCHVLGPAHPPDPQRHPNTLSFRCSLADFQIEKKIGRGQFSEVYKATCLLDRKTVALKKVQIFEMMDAKARQDCVKEIGLLKQLNHPNIIKYLDSFIEDNELNIVLELADAGDLSQMIKYFKKQKRLIPERTVWKYFVQLCSAVEHMHSRRVMHRDIKPANVFITATGIVKLGDLGLGRFFSSETTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLFSLCQKIEQCDYPPLPGEHYSEKLRELVSMCIYPDPNHRPDIEYVHQVAKQMHVWTSST
InterPro domains: - IPR008271: Serine/threonine-protein kinase, active site (active_site) [168-180]
- IPR001245: Serine-threonine/tyrosine-protein kinase, catalytic domain (domain) [122-296]
- IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [29-301]
- IPR017441: Protein kinase, ATP binding site (binding_site) [51-74]
- IPR000719: Protein kinase domain (domain) [45-310]
Interaction partners: - RCC1-like G exchanging factor-like protein
- Serine/threonine-protein kinase Nek9
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0023056 (positive regulation of signaling), GO:0042221 (response to chemical), GO:0048584 (positive regulation of response to stimulus), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0009893 (positive regulation of metabolic process), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0006950 (response to stress), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0009967 (positive regulation of signal transduction), GO:1901564 (organonitrogen compound metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0080134 (regulation of response to stress), GO:0019538 (protein metabolic process), GO:0080135 (regulation of cellular response to stress), GO:0009966 (regulation of signal transduction), GO:0060255 (regulation of macromolecule metabolic process), GO:1901700 (response to oxygen-containing compound), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0006793 (phosphorus metabolic process), GO:0051726 (regulation of cell cycle), GO:0010035 (response to inorganic substance), GO:0010646 (regulation of cell communication), GO:0031325 (positive regulation of cellular metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0010647 (positive regulation of cell communication), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:1902074 (response to salt), GO:0010038 (response to metal ion), GO:0007346 (regulation of mitotic cell cycle), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0071310 (cellular response to organic substance), GO:0043067 (regulation of programmed cell death), GO:0062197 (cellular response to chemical stress), GO:0006796 (phosphate-containing compound metabolic process), GO:0070542 (response to fatty acid), GO:0010468 (regulation of gene expression), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0071241 (cellular response to inorganic substance), GO:0031326 (regulation of cellular biosynthetic process), GO:0051247 (positive regulation of protein metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:1902533 (positive regulation of intracellular signal transduction), GO:0051252 (regulation of RNA metabolic process), GO:1901701 (cellular response to oxygen-containing compound), GO:1902531 (regulation of intracellular signal transduction), GO:0051174 (regulation of phosphorus metabolic process), GO:0043408 (regulation of MAPK cascade), GO:0014066 (regulation of phosphatidylinositol 3-kinase signaling), GO:0042981 (regulation of apoptotic process), GO:0031399 (regulation of protein modification process), GO:0071277 (cellular response to calcium ion), GO:0045937 (positive regulation of phosphate metabolic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0006468 (protein phosphorylation), GO:0006355 (regulation of DNA-templated transcription), GO:0019220 (regulation of phosphate metabolic process), GO:0016310 (phosphorylation), GO:0071248 (cellular response to metal ion), GO:0043410 (positive regulation of MAPK cascade), GO:0046686 (response to cadmium ion), GO:0043410 (positive regulation of MAPK cascade), GO:0031401 (positive regulation of protein modification process), GO:0042325 (regulation of phosphorylation), GO:0001932 (regulation of protein phosphorylation), GO:0042327 (positive regulation of phosphorylation), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0001934 (positive regulation of protein phosphorylation), GO:0046777 (protein autophosphorylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)