Protein: Replication factor C subunit 4 (Replication factor C4) (Activator 1 37 kDa subunit) (P40339)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Component of ATP-dependent clamp loader (RFC and RFC-like) complexes for DNA clamps, such as the POL30/PCNA homotrimer and the checkpoint clamp DDC1:MEC3:RAD17 complex. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Component of the replication factor C (RFC or activator 1) complex which loads POL30/PCNA and acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. Component of the RFC-like ELG1-RFC complex which appears to have a role in DNA replication, replication fork re-start, recombination and repair.
Subcellular location: Nucleus {ECO:0000305}.
Sequence: MSKTLSLQLPWVEKYRPQVLSDIVGNKETIDRLQQIAKDGNMPHMIISGMPGIGKTTSVHCLAHELLGRSYADGVLELNASDDRGIDVVRNQIKHFAQKKLHLPPGKHKIVILDEADSMTAGAQQALRRTMELYSNSTRFAFACNQSNKIIEPLQSRCAILRYSKLSDEDVLKRLLQIIKLEDVKYTNDGLEAIIFTAEGDMRQAINNLQSTVAGHGLVNADNVFKIVDSPHPLIVKKMLLASNLEDSIQILRTDLWKKGYSSIDIVTTSFRVTKNLAQVKESVRLEMIKEIGLTHMRILEGVGTYLQLASMLAKIHKLNNKA
InterPro domains: - IPR003593: AAA+ ATPase domain (domain) [41-255]
- IPR050238: DNA Replication and Repair Clamp Loader Complex (family) [9-311]
- IPR003959: ATPase, AAA-type, core (domain) [45-163]
- IPR047854: Replication factor C, lid domain (domain) [166-228]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [2-228]
- IPR013748: Replication factor C, C-terminal (domain) [231-315]
- IPR008921: DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal (homologous_superfamily) [231-321]
Interaction partners: - Proliferating cell nuclear antigen
- Replication factor C subunit 2
- Replication factor C subunit 3
- Telomere length regulation protein ELG1
- Replication factor C subunit 5
- Replication factor C subunit 1
- Checkpoint protein RAD24
- Chromosome transmission fidelity protein 18
- DNA damage checkpoint protein 1
- Sister chromatid cohesion protein DCC1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0016887 (ATP hydrolysis activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0008094 (ATP-dependent activity, acting on DNA), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:0140097 (catalytic activity, acting on DNA), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0016462 (pyrophosphatase activity), GO:0017111 (ribonucleoside triphosphate phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0006259 (DNA metabolic process), GO:0022616 (DNA strand elongation), GO:0006260 (DNA replication), GO:0006281 (DNA repair), GO:0006261 (DNA-templated DNA replication), GO:0006271 (DNA strand elongation involved in DNA replication), GO:0006298 (mismatch repair)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005657 (replication fork), GO:0005829 (cytosol), GO:1902494 (catalytic complex), GO:0140513 (nuclear protein-containing complex), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome), GO:0005634 (nucleus)