Protein: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 3, chloroplastic (EC 2.1.1.14) (Cobalamin-independent methionine synthase 3) (AtMS3) (Q0WNZ5)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.
Subcellular location: Plastid, chloroplast {ECO:0000269|PubMed:15024005}.
Sequence: MGQLALQRLQPLASLPRRPPSLPPPSSATPSLPCATASRRPRFYVARAMSSHIVGYPRIGPKRELKFALESFWDGKTNVDDLQNVAANLRKSIWKHMAHAGIKYIPSNTFSYYDQMLDTTAMLGAVPSRYGWESGEIGFDVYFSMARGNASAHAMEMTKWFDTNYHYIVPELGPDVNFSYASHKAVVEFKEAKALGIDTVPVLIGPMTYLLLSKPAKGVEKSFCLLSLIDKILPVYKEVLADLKSAGARWIQFDEPILVMDLDTSQLQAFSDAYSHMESSLAGLNVLIATYFADVPAEAYKTLMSLKCVTGFGFDLVRGLETLDLIKMNFPRGKLLFAGVVDGRNIWANDLSASLKTLQTLEDIVGKEKVVVSTSCSLLHTAVDLVNEMKLDKELKSWLAFAAQKVVEVNALAKSFSGAKDEALFSSNSMRQASRRSSPRVTNAAVQQDVDAVKKSDHHRSTEVSVRLQAQQKKLNLPALPTTTIGSFPQTTDLRRIRREFKAKKISEVDYVQTIKEEYEKVIKLQEELGIDVLVHGEAERNDMVEFFGEQLSGFAFTSNGWVQSYGSRCVKPPIIYGDITRPKAMTVFWSSMAQKMTQRPMKGMLTGPVTILNWSFVRNDQPRHETCFQIALAIKDEVEDLEKAGVTVIQIDEAALREGLPLRKSEQKFYLDWAVHAFRITNSGVQDSTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFHEGVKYGAGIGPGVYDIHSPRIPSTEEIAERINKMLAVLDSKVLWVNPDCGLKTRNYSEVKSALSNMVAAAKLIRSQLNKS
InterPro domains: - IPR002629: Cobalamin-independent methionine synthase MetE, C-terminal/archaeal (domain) [480-804]
- IPR013215: Cobalamin-independent methionine synthase MetE, N-terminal (domain) [51-364]
- IPR038071: UROD/MetE-like superfamily (homologous_superfamily) [51-807]
- IPR006276: Cobalamin-independent methionine synthase (family) [48-812]
Interaction partners: - Homocysteine S-methyltransferase 1
- Adenosylhomocysteinase 2
- Homocysteine S-methyltransferase 2
- Homocysteine S-methyltransferase 3
- Adenosylhomocysteinase 1
- Cystathionine beta-lyase, chloroplastic
- S-adenosylmethionine synthase 2
- Probable cystathionine gamma-synthase 2
- Cystathionine gamma-synthase 1, chloroplastic
- S-adenosylmethionine synthase 3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0043169 (cation binding), GO:0046872 (metal ion binding), GO:0008168 (methyltransferase activity), GO:0003723 (RNA binding), GO:0003729 (mRNA binding), GO:0008172 (S-methyltransferase activity), GO:0046914 (transition metal ion binding), GO:0005507 (copper ion binding), GO:0008270 (zinc ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006575 (cellular modified amino acid metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0006082 (organic acid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0006730 (one-carbon metabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0000096 (sulfur amino acid metabolic process), GO:0044272 (sulfur compound biosynthetic process), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:1901657 (glycosyl compound metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0050667 (homocysteine metabolic process), GO:0009066 (aspartate family amino acid metabolic process), GO:0000097 (sulfur amino acid biosynthetic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0006555 (methionine metabolic process), GO:0009086 (methionine biosynthetic process), GO:0009067 (aspartate family amino acid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0009532 (plastid stroma), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0005739 (mitochondrion), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009570 (chloroplast stroma), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle)