Protein: Large ribosomal subunit protein uL5 (60S ribosomal protein L11) (Q29205)
Organism: Sus scrofa (Pig)
Function: Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53. Promotes nucleolar location of PML.
Subcellular location: Nucleus, nucleolus {ECO:0000250|UniProtKB:Q9CXW4}. Cytoplasm {ECO:0000250|UniProtKB:Q9CXW4}.
Sequence: MAQDQGEKENPMRELRIRKLCLNICVGESGDRLTRAAKVLEQLTGQTPVFSKARYTVRSFGIRRNEKIAVHCTVRGAKAEEILEKGLKVREYELRKNNFSDTGNFGFGIQEHIDLGIKYDPSIGIYGLDFYVVLGRPGFSIADKKRRTGCIGAKHRISKEEAMRWFQQKHDGIILPGN
InterPro domains: - IPR002132: Large ribosomal subunit protein uL5 (family) [1-176]
- IPR031309: Large ribosomal subunit protein uL5, C-terminal (domain) [67-165]
- IPR057266: Large ribosomal subunit protein uL5, eukaryotic/archaeal-type (family) [5-171]
- IPR020929: Large ribosomal subunit protein uL5, conserved site (conserved_site) [40-56]
- IPR022803: Large ribosomal subunit protein uL5 domain superfamily (homologous_superfamily) [1-178]
- IPR031310: Large ribosomal subunit protein uL5, N-terminal (domain) [10-63]
Interaction partners: - Large ribosomal subunit protein eL27
- Large ribosomal subunit protein uL18
- Large ribosomal subunit protein uL29
- Large ribosomal subunit protein uL30
- Uncharacterized protein.
- Ribosomal protein L24.
- Large ribosomal subunit protein uL4
- Large ribosomal subunit protein eL30
- Large ribosomal subunit protein eL38
- Large ribosomal subunit protein eL6
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044238 (primary metabolic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0019538 (protein metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006412 (translation), GO:0009059 (macromolecule biosynthetic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0006518 (peptide metabolic process), GO:0010467 (gene expression), GO:0043043 (peptide biosynthetic process), GO:0002181 (cytoplasmic translation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0098552 (side of membrane), GO:1990904 (ribonucleoprotein complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0031984 (organelle subcompartment), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0043229 (intracellular organelle), GO:0098562 (cytoplasmic side of membrane), GO:0043228 (non-membrane-bounded organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0044391 (ribosomal subunit), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005791 (rough endoplasmic reticulum), GO:0005789 (endoplasmic reticulum membrane), GO:0098554 (cytoplasmic side of endoplasmic reticulum membrane), GO:0015934 (large ribosomal subunit), GO:0098556 (cytoplasmic side of rough endoplasmic reticulum membrane), GO:0030867 (rough endoplasmic reticulum membrane), GO:0005840 (ribosome)