Protein: Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (EC 1.14.11.4) (Lysyl hydroxylase 1) (LH1) (Q63321)
Organism: Rattus norvegicus (Rat)
Function: Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (By similarity). Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (By similarity).
Subcellular location: Rough endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side.
Sequence: MRSLLLLASLAWLLLAQAKDDAKLEDNLLVLTVATKETEGFRRFKRSAQFFNYKIQSLGLGEDWSAAGGPSAAGGGQKVRLLKKALKKYADKEDLVILFVDSYDVVFASGPRELLKKFQQAKSRVVFSAEELIYPDRRLEAKYPTVPDGKRFLGSGGFIGYAPSLSKLVAEWEGQDNDSDQLFYTKIFLDPEKREQINISLDHRCRIFQNLDGALDEVVLKFEMGHVRARNLAYDTLPVVIHGNGPTKLQVNYLGNYIPRFWTFETGCTVCDEGLRSLKGIGDEALPTVLVGVFIEQPTPFLSLFFRRLLHLRYPQKQMRLFIHNQEQHHKLQVEQFLAEHGGEYQSVKLVGPEVRMANADARNMGADLCRQDQTCTYYFSVDADVALTEPNSLRLLIEQNKNVIAPLMTRHGRLWSNFWGALSADGYYARSEDYVDIVQGRRVGVWNVPYISNIYLIKGSALRAELRHVDLFHYSKLDPDMSFCANVRQQEVFMFLTNRHTFGHLLSLDNYQTTHLHNDLWEVFSNPQDWKEKYIHENYTKALAGKLVETPCPDVYWFPIFTEVACDELVEEMEHYGQWSLGDNKDNRIQGGYENVPTIDIHMNQITFEREWHKFLVEYIAPLTEKLYPGYYTKAQFDLAFVVRYKPDEQPSLMPHHDASTFTINIALNRVGEDYEGGGCRFLRYNCSVRAPRKGWALMHPGRLTHYHEGLPTTKGTRYIAVSFVDP
InterPro domains: - IPR029044: Nucleotide-diphospho-sugar transferases (homologous_superfamily) [302-602]
- IPR057589: PLOD1-3-like, GT domain (domain) [25-268]
- IPR005123: Oxoglutarate/iron-dependent dioxygenase domain (domain) [637-728]
- IPR050757: Collagen-modifying Glycosyltransferase 25 (family) [57-721]
- IPR001006: Procollagen-lysine 5-dioxygenase, conserved site (conserved_site) [656-663]
- IPR044861: Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain (domain) [639-728]
- IPR006620: Prolyl 4-hydroxylase, alpha subunit (domain) [554-727]
Interaction partners: - Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2
- Glycosyltransferase 25 domain containing 1
- Collagen beta
- Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3
- Calmodulin-lysine N-methyltransferase
- Prolyl 4-hydroxylase subunit alpha 2
- Collagen type V alpha 2 chain
- Prolyl 4-hydroxylase subunit alpha-1
- Collagen alpha-1
- Histone-lysine N-methyltransferase, H3 lysine-36 specific
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0043167 (ion binding), GO:0005515 (protein binding), GO:0016491 (oxidoreductase activity), GO:0051213 (dioxygenase activity), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0043177 (organic acid binding), GO:0043168 (anion binding), GO:0043169 (cation binding), GO:0031406 (carboxylic acid binding), GO:0016706 (2-oxoglutarate-dependent dioxygenase activity), GO:0046872 (metal ion binding), GO:0046914 (transition metal ion binding), GO:0005506 (iron ion binding), GO:0008198 (ferrous iron binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0051716 (cellular response to stimulus), GO:0009628 (response to abiotic stimulus), GO:0006950 (response to stress), GO:0042221 (response to chemical), GO:0009719 (response to endogenous stimulus), GO:0071495 (cellular response to endogenous stimulus), GO:0001666 (response to hypoxia), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:0070887 (cellular response to chemical stimulus), GO:0070482 (response to oxygen levels), GO:0032870 (cellular response to hormone stimulus), GO:0071310 (cellular response to organic substance), GO:0036293 (response to decreased oxygen levels)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005615 (extracellular space), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031984 (organelle subcompartment), GO:0005576 (extracellular region), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0031090 (organelle membrane), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0043231 (intracellular membrane-bounded organelle), GO:0005789 (endoplasmic reticulum membrane)