Protein: Exonuclease mut-7 (EC 3.1.-.-) (Exonuclease 3'-5' domain-containing protein 3 homolog) (P34607)
Organism: Caenorhabditis elegans
Function: Represses the transposition of Tc1, Tc3, Tc4, and Tc5, perhaps by degrading transposon-specific messages. Also affects sperm development, sensitivity to RNAi of mainly germline expressed genes, silencing of some germline transgenes, X chromosome loss, and is required for cosuppression (functional silencing of chromosomal loci induced by transgenes) and for silencing induced by antisense RNA oligomers.
Sequence: MEEEPYKRKLTKAEKKAKYRTDYAEPLKSRREVLKAIMNGPESERERKVRAKNREFFNEDYRSGVNIYGMAVDMMKAMPDRGKTSGQSLAVWYLEDFGVWLKESGQETELRQKYLTGTIQINALDVCTIGQKQLLSEIFDITKEKFTEDITQLLDAAIKKQDFSVAADMAIQYNLLRDHHFEHLVLPLMLSGKDQTAYKLISNNERMQQQLVEFFDRMVGISVVAVEEMLKPYKETKIMTIPMEKLTGKTLDKLISTIINKNTHEYNFSRELSKFAKNHSQNGNLKALKFNISERYEKGKSDDNYFQHMVETFTKAEDVREPILFYLWSSNDTEKQIDAICFAIYLGIASSSSYQLPNVMRDFFRQPDSKLREAKELLVRRKTLQVPLNGEQLFVFENERRTQIHMVKTESEMNYLCSEIKSLSDEPAPVYVGFDSEWKPSNLTAVHDSKIAIIQLFFKNCVWLVDCVELEKANMADDWWQKFASRLFGDSPVKVVGFDMRNDLDAMATIPALKSSMKIEDTKNAFDLKRLAENVCDIDMEILELPKKTFKLADLTHYLLGLELDKTEQCSNWQCRPLRKKQIVYAALDAVVVVETFKKILSIVEEKNKDADIEKIVRESNVMAPKKDKGHKSYRKLKTIPWLELYDILRSHRNPTRSPQRPHDIKVIVDTMLIGFGKNLRRVGIDVILPKDVSDFRKYLKEIERVGGEHLRHIITVPSKSYEALKMDYDNYTIAIPELNNMSPVDQLIEFFDLFNVDIRPEDVYPRCTECNSRLQIKFPGPVLHFLHQYCVIHVQNVYRADMSEFPLEEWWNRMLHINPDDYDGVKVEMSRPSPTSKWIVATVPTGCLHITRQTALHTNLPDGIEVRIHKVPDDEFKRRNLSFYVCGECGTVACDGRGNQASESTSQEC
InterPro domains: - IPR052408: Exonuclease MUT-7-like (family) [52-606]
- IPR002782: Mut7-C RNAse domain (domain) [666-779]
- IPR002562: 3'-5' exonuclease domain (domain) [404-605]
- IPR037432: Exonuclease Mut-7, DEDDy 3'-5' exonuclease domain (domain) [404-602]
- IPR012337: Ribonuclease H-like superfamily (homologous_superfamily) [386-637]
- IPR036397: Ribonuclease H superfamily (homologous_superfamily) [395-614]
Interaction partners: - SH2 domain-containing protein
- PAP-associated domain-containing protein
- MUTator
- MUTator
- RNA helicase
- DRBM domain-containing protein
- RNA-directed RNA polymerase
- Piwi domain-containing protein
- Piwi-like protein 1
- RNase NYN domain-containing protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0005515 (protein binding), GO:0140098 (catalytic activity, acting on RNA), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0140097 (catalytic activity, acting on DNA), GO:0004518 (nuclease activity), GO:0004536 (deoxyribonuclease activity), GO:0004540 (ribonuclease activity), GO:0004527 (exonuclease activity), GO:0004520 (DNA endonuclease activity), GO:0004529 (DNA exonuclease activity), GO:0004519 (endonuclease activity), GO:0008408 (3'-5' exonuclease activity), GO:0016896 (RNA exonuclease activity, producing 5'-phosphomonoesters), GO:0016796 (exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters), GO:0008296 (3'-5'-DNA exonuclease activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0009892 (negative regulation of metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression), GO:0016070 (RNA metabolic process), GO:0031047 (RNA-mediated gene silencing), GO:0006396 (RNA processing), GO:0034470 (ncRNA processing), GO:0070918 (regulatory ncRNA processing), GO:0034660 (ncRNA metabolic process), GO:0035196 (miRNA processing), GO:0031054 (pre-miRNA processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)