Protein: Long-chain specific acyl-CoA dehydrogenase, mitochondrial (LCAD) (EC 1.3.8.8) (P51174)
Organism: Mus musculus (Mouse)
Function: Long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, long-chain specific acyl-CoA dehydrogenase can act on saturated and unsaturated acyl-CoAs with 6 to 24 carbons with a preference for 8 to 18 carbons long primary chains.
Subcellular location: Mitochondrion matrix {ECO:0000250|UniProtKB:P15650}.
Sequence: MAARLLLRSLRVLKARSAPRPPPSARCSHSGAEARLETPSAKKLTDVGIRRIFSSEHDIFRESVRKFFQEEVIPHHTEWEKAGEVSREVWEKAGKQGLLGINIAEKHGGIGGDLLSTAVTWEEQAYSNCTGPGFSLHSDIVMPYIANYGTKEQIEKFIPQMTAGKCIGAIAMTEPGAGSDLQGVRTNAKRSGSDWILNGSKVFITNGWLSDLVIVVAVTNREARSPAHGISLFLVENGMKGFIKGRKLHKMGMKAQDTAELFFEDVRLPANALLGEENKGFYYLMQELPQERLLIAELAISACEFMFEETRNYVKQRKAFGKTVAHIQTVQHKLAELKTHICVTRAFVDSCLQLHETKRLDSGSASMAKYWASELQNSVAYECVQLHGGWGYMWEYPIAKAYVDARVQPIYGGTNEIMKELIARQIVSDS
InterPro domains: - IPR037069: Acyl-CoA dehydrogenase/oxidase, N-terminal domain superfamily (homologous_superfamily) [32-166]
- IPR034179: Long-chain specific acyl-CoA dehydrogenase (family) [56-427]
- IPR036250: Acyl-CoA dehydrogenase-like, C-terminal (homologous_superfamily) [276-427]
- IPR013786: Acyl-CoA dehydrogenase/oxidase, N-terminal (domain) [55-165]
- IPR006091: Acyl-CoA oxidase/dehydrogenase, middle domain (domain) [169-266]
- IPR050741: Acyl-CoA dehydrogenase (family) [50-427]
- IPR009100: Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain superfamily (homologous_superfamily) [50-290]
- IPR006089: Acyl-CoA dehydrogenase, conserved site (conserved_site) [171-404]
- IPR046373: Acyl-CoA oxidase/dehydrogenase, middle domain superfamily (homologous_superfamily) [168-283]
- IPR009075: Acyl-CoA dehydrogenase/oxidase, C-terminal (domain) [278-426]
Interaction partners: - Trifunctional enzyme subunit beta, mitochondrial
- Trifunctional enzyme subunit alpha, mitochondrial
- 3-ketoacyl-CoA thiolase, mitochondrial
- Carnitine O-palmitoyltransferase 2, mitochondrial
- 3-ketoacyl-CoA thiolase B, peroxisomal
- Enoyl-CoA hydratase, mitochondrial
- Peroxisomal acyl-coenzyme A oxidase 1
- 3-ketoacyl-CoA thiolase A, peroxisomal
- Peroxisomal bifunctional enzyme
- Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0005488 (binding), GO:0036094 (small molecule binding), GO:0008289 (lipid binding), GO:1901681 (sulfur compound binding), GO:0097159 (organic cyclic compound binding), GO:0016491 (oxidoreductase activity), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0033218 (amide binding), GO:0097367 (carbohydrate derivative binding), GO:0005515 (protein binding), GO:1901265 (nucleoside phosphate binding), GO:0016627 (oxidoreductase activity, acting on the CH-CH group of donors), GO:1901567 (fatty acid derivative binding), GO:0043168 (anion binding), GO:0042802 (identical protein binding), GO:0120227 (acyl-CoA binding), GO:0000166 (nucleotide binding), GO:0032553 (ribonucleotide binding), GO:0000062 (fatty-acyl-CoA binding), GO:0017076 (purine nucleotide binding), GO:0050660 (flavin adenine dinucleotide binding), GO:0052890 (oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor), GO:0032555 (purine ribonucleotide binding), GO:0032559 (adenyl ribonucleotide binding), GO:0003995 (acyl-CoA dehydrogenase activity), GO:0030554 (adenyl nucleotide binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0006629 (lipid metabolic process), GO:0044282 (small molecule catabolic process), GO:1901575 (organic substance catabolic process), GO:0044248 (cellular catabolic process), GO:0006082 (organic acid metabolic process), GO:0030258 (lipid modification), GO:0006631 (fatty acid metabolic process), GO:0016054 (organic acid catabolic process), GO:0044242 (cellular lipid catabolic process), GO:0043436 (oxoacid metabolic process), GO:0016042 (lipid catabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0046395 (carboxylic acid catabolic process), GO:0034440 (lipid oxidation), GO:0009062 (fatty acid catabolic process), GO:0019395 (fatty acid oxidation), GO:0072329 (monocarboxylic acid catabolic process), GO:0006635 (fatty acid beta-oxidation), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)