Protein: Kinetochore protein NUF2 (P33895)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Acts as component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.
Subcellular location: Nucleus. Chromosome, centromere, kinetochore. Note=Associated with kinetochores.
Sequence: MSRNQDVFPILDLQELVICLQSCDFALATQENISRPTSDYMVTLYKQIIENFMGISVESLLNSSNQETGDGHLQEENENIYLDTLNVLVLNKICFKFFENIGVQDFNMTDLYKPEAQRTQRLLSAVVNYARFREERMFDCNSFILQMESLLGQLRSKFDDYNLIQQQLKQYEDVDGDNIPDEQELQKLEEQNKELEIQLKKLTKIQETLSIDYNDYKISKQSIFKDLEALSFQIVELESNRDKLIKISNTDMEELSEGIKELNDLLIQRKKTLDDLTAQQKNLQDTVTTFETIISELYDVLRIISSEVQESNRTETELVGLKQNLINNKLKLMNVLETGIMYKLEILQEQLDLQLKNLEKLSQDTKEESRLNDTKLMDLQIKYENEIKPKIDKTDIFIQEELISGKINKLNDEIKQLQKDFEVEVKEIEIEYSLLSGHINKYMNEMLEYMQ
InterPro domains: - IPR005549: Kinetochore protein Nuf2, N-terminal (domain) [6-148]
- IPR038275: Nuf2, N-terminal domain superfamily (homologous_superfamily) [1-168]
Interaction partners: - Kinetochore protein SPC24
- Kinetochore protein NDC80
- Kinetochore protein SPC25
- Protein STU2
- Outer kinetochore KNL1 complex subunit SPC105
- Serine/threonine-protein kinase MPS1
- Kinetochore-associated protein DSN1
- Mitotic spindle checkpoint component MAD2
- Spindle assembly checkpoint serine/threonine-protein kinase BUB1
- Kinetochore-associated protein NNF1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0044877 (protein-containing complex binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0051641 (cellular localization), GO:0031503 (protein-containing complex localization), GO:0050794 (regulation of cellular process), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0007017 (microtubule-based process), GO:0048523 (negative regulation of cellular process), GO:0042073 (intraciliary transport), GO:0007018 (microtubule-based movement), GO:0051129 (negative regulation of cellular component organization), GO:0046907 (intracellular transport), GO:0006810 (transport), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0051128 (regulation of cellular component organization), GO:0044087 (regulation of cellular component biogenesis), GO:0010970 (transport along microtubule), GO:0043254 (regulation of protein-containing complex assembly), GO:0030705 (cytoskeleton-dependent intracellular transport), GO:0033043 (regulation of organelle organization), GO:0006996 (organelle organization), GO:1902115 (regulation of organelle assembly), GO:0099111 (microtubule-based transport), GO:0072384 (organelle transport along microtubule), GO:0030473 (nuclear migration along microtubule), GO:1902116 (negative regulation of organelle assembly), GO:0051494 (negative regulation of cytoskeleton organization), GO:0047496 (vesicle transport along microtubule), GO:0099518 (vesicle cytoskeletal trafficking)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0000940 (outer kinetochore), GO:0098687 (chromosomal region), GO:0099080 (supramolecular complex), GO:0043229 (intracellular organelle), GO:0000776 (kinetochore), GO:0043228 (non-membrane-bounded organelle), GO:0000775 (chromosome, centromeric region), GO:0099081 (supramolecular polymer), GO:0000779 (condensed chromosome, centromeric region), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0099512 (supramolecular fiber), GO:0099513 (polymeric cytoskeletal fiber), GO:0005694 (chromosome), GO:0000793 (condensed chromosome)