Protein: Eukaryotic translation initiation factor 3 subunit E (eIF3e) (Eukaryotic translation initiation factor 3 subunit 6) (O77410)
Organism: Drosophila melanogaster (Fruit fly)
Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. In addition to its role in the eIF-3 complex, also functions in protein ubiquitination and degradation. During mitosis required for regulating mitotic microtubule growth and kinetochore formation, and consequently is required for satisfying the spindle assembly checkpoint (SAC) during metaphase to prevent delays in mitotic progression. This is likely by promoting the ubiquitination and degradation of Klp67A, a kinesin-like protein that suppresses microtubule polymerization at plus ends. Acts in the COP9 signalosome (CSN) mediated regulation of cullin neddylation by promoting Cul1 and Cul3 neddylation and negatively regulating the CSN complex subunit CSN5.
Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03004, ECO:0000269|PubMed:28505193}. Microsome {ECO:0000269|PubMed:10375641}. Endoplasmic reticulum {ECO:0000269|PubMed:10375641}. Note=Uniformly distributed in interphase and mitotic cells. {ECO:0000269|PubMed:28505193}.
Sequence: MANFDLTRINCQFLDRHLTFPLLEFLCGKEIYNQQELLEYILETVNKTNMIDYTMDTRKRLNLSQEMPEELVQRKAEVLATLKQLQNEVAPIMKATDILKNGESMKDSKTFVNALQKDYNFKVEHLESAYKLAKYLYECGNYQESTSYLYFCLIVMSPNDKNYLNVLWGKLAAEILTLNWNTALEDLTRLRDYIDNANFSTIQALQQRTWLIHWSVLVFFNHPKGRDLIIEMFLYKPLYLNAIQTMCPHIMRYLATAVVINRTRRNALKDLIKVIQQESYTYRDPITEFLECLYVNFDFEGARLKLHECQTVILNDFFIVACLNEFVEDARLMIFETFCRIHQCITISMLADKLNMKPNEAECWIVNLIRNARLNAKIDSKLGHVVMGTQPLSPYQQLVEKIDSLSMRSEHLAGLIERKSKQKQNQESADSWKYY
InterPro domains: - IPR036390: Winged helix DNA-binding domain superfamily (homologous_superfamily) [315-389]
- IPR000717: Proteasome component (PCI) domain (domain) [219-405]
- IPR016650: Eukaryotic translation initiation factor 3 subunit E (family) [1-435]
- IPR019010: Eukaryotic translation initiation factor 3 subunit E, N-terminal (domain) [5-136]
Interaction partners: - Eukaryotic translation initiation factor 3 subunit L
- Eukaryotic translation initiation factor 3 subunit I
- Eukaryotic translation initiation factor 3 subunit C
- Eukaryotic translation initiation factor 3 subunit M
- Eukaryotic translation initiation factor 3 subunit A
- Eukaryotic translation initiation factor 3 subunit B
- Eukaryotic translation initiation factor 3 subunit K
- Eukaryotic translation initiation factor 3 subunit H
- Eukaryotic translation initiation factor 3 subunit G-1
- Eukaryotic translation initiation factor 3 subunit D-1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0045182 (translation regulator activity), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:0090079 (translation regulator activity, nucleic acid binding), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0008135 (translation factor activity, RNA binding), GO:0003743 (translation initiation factor activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0019953 (sexual reproduction), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0009058 (biosynthetic process), GO:0009653 (anatomical structure morphogenesis), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0032504 (multicellular organism reproduction), GO:0003006 (developmental process involved in reproduction), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0044237 (cellular metabolic process), GO:0022412 (cellular process involved in reproduction in multicellular organism), GO:0048522 (positive regulation of cellular process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0065009 (regulation of molecular function), GO:0044238 (primary metabolic process), GO:0007276 (gamete generation), GO:0050790 (regulation of catalytic activity), GO:0007281 (germ cell development), GO:0044093 (positive regulation of molecular function), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0007349 (cellularization), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0019538 (protein metabolic process), GO:0048515 (spermatid differentiation), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0007283 (spermatogenesis), GO:0007291 (sperm individualization), GO:0031325 (positive regulation of cellular metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0043603 (amide metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0006518 (peptide metabolic process), GO:0006508 (proteolysis), GO:0051246 (regulation of protein metabolic process), GO:0043604 (amide biosynthetic process), GO:0007286 (spermatid development), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006412 (translation), GO:0051247 (positive regulation of protein metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0051338 (regulation of transferase activity), GO:0043085 (positive regulation of catalytic activity), GO:0048232 (male gamete generation), GO:0010467 (gene expression), GO:0051347 (positive regulation of transferase activity), GO:0030162 (regulation of proteolysis), GO:0043043 (peptide biosynthetic process), GO:0031399 (regulation of protein modification process), GO:0002181 (cytoplasmic translation), GO:0051438 (regulation of ubiquitin-protein transferase activity), GO:0045862 (positive regulation of proteolysis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005852 (eukaryotic translation initiation factor 3 complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)