Protein: Cytochrome P450 94B1 (EC 1.14.14.48) (Jasmonoyl-L-amino acid 12-hydroxylase) (Q9FMV7)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Hydroxylase involved in the oxidation of the plant hormone jasmonoyl-L-isoleucine (JA-Ile), a bioactive phytohormone of the jasmonate-mediated signaling pathway. Converts JA-Ile to 12-hydroxy-JA-Ile.
Subcellular location: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
Sequence: MEMLNAIILILFPIIGFVLIFSFPTKTLKAKTASPSNPTSYQLIGSILSFNKNRHRLLQWYTDLLRLSPSQTITVDLLFGRRTIITANPENVEHILKTNFYNFPKGKPFTDLLGDLLGGGIFNSDGELWSSQRKLASHEFTMRSLREFTFEILREEVQNRLIPVLSSAVDCGETVDFQEVLKRFAFDVVCKVSLGWDPDCLDLTRPVPELVKAFDVAAEISARRATEPVYAVWKVKRFLNVGSEKRLREAIKTVHLSVSEIIRAKKKSLDIGGDVSDKQDLLSRFLAAGHGEEAVRDSVISFIMAGRDTTSAAMTWLFWLLSQNDDVETKILDELRNKGSLGLGFEDLREMSYTKACLCEAMRLYPPVAWDSKHAANDDILPDGTPLKKGDKVTYFPYGMGRMEKVWGKDWDEFKPNRWFEEEPSYGTKPVLKSVSSFKFPVFQAGPRVCIGKEMAFTQMKYVVGSVLSRFKIIPVCNNRPVFVPLLTAHMAGGLKVKIKRREQCDSMYI
InterPro domains: - IPR001128: Cytochrome P450 (family) [35-475]
- IPR002401: Cytochrome P450, E-class, group I (family) [294-473]
- IPR036396: Cytochrome P450 superfamily (homologous_superfamily) [26-505]
Interaction partners: - IAA-amino acid hydrolase ILR1-like 6
- IAA-amino acid hydrolase ILR1-like 4
- Cytosolic sulfotransferase 15
- Probable long-chain-alcohol O-fatty-acyltransferase 6
- KIB1-4 beta-propeller domain-containing protein
- 60S ribosomal L18a-like protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0004497 (monooxygenase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0022414 (reproductive process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0009605 (response to external stimulus), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0009607 (response to biotic stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0003006 (developmental process involved in reproduction), GO:0051707 (response to other organism), GO:0044238 (primary metabolic process), GO:0009791 (post-embryonic development), GO:0099402 (plant organ development), GO:0043207 (response to external biotic stimulus), GO:0006629 (lipid metabolic process), GO:0009611 (response to wounding), GO:0090567 (reproductive shoot system development), GO:0006082 (organic acid metabolic process), GO:0048731 (system development), GO:0006952 (defense response), GO:0044255 (cellular lipid metabolic process), GO:0048608 (reproductive structure development), GO:0048437 (floral organ development), GO:0048438 (floral whorl development), GO:0048229 (gametophyte development), GO:0048653 (anther development), GO:0098542 (defense response to other organism), GO:0048443 (stamen development), GO:0048367 (shoot system development), GO:0061458 (reproductive system development), GO:0006631 (fatty acid metabolic process), GO:0009555 (pollen development), GO:0043436 (oxoacid metabolic process), GO:0048466 (androecium development), GO:0048827 (phyllome development), GO:0048467 (gynoecium development), GO:0048440 (carpel development), GO:0002213 (defense response to insect), GO:0010154 (fruit development), GO:0009908 (flower development), GO:0019752 (carboxylic acid metabolic process), GO:0001676 (long-chain fatty acid metabolic process), GO:0009694 (jasmonic acid metabolic process), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)