Protein: Hydroxyproline O-galactosyltransferase HPGT2 (EC 2.4.1.-) (Beta-1,3-galactosyltransferase 10) (Q94A05)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Possesses hydroxyproline O-galactosyltransferase activity. Transfers galactose from UDP-galactose to hydroxyproline residues in the arabinogalactan proteins (AGPs). Is specific for AGPs containing non-contiguous peptidyl hydroxyproline residues. The addition of galactose onto the peptidyl hydroxyproline residues in AGP core proteins represents the first committed step in arabinogalactan polysaccharide addition. AGP glycans play essential roles in both vegetative and reproductive plant growth.
Subcellular location: Golgi apparatus membrane {ECO:0000250|UniProtKB:Q94F27}; Single-pass type II membrane protein {ECO:0000305}.
Sequence: MESLPTTVSGKSDRRGRFSKSQNTSKPSLILAFFSCLAWLYVAGRLWQDAQYRAALNTVLKMNYDQRPKVLTVEDKLVVLGCKDLERRIVETEMELAQAKSQGYLKKQKSVSSSGKKMLAVIGVYTGFGSHLKRNKFRGSWMPRDDALKKLEERGVVIRFVIGRSANRGDSLDRKIDEENRATKDFLILENHEEAQEELPKKVKFFYSAAVQNWDAEFYVKVDDNVDLDLEGMIALLESRRSQDGAYIGCMKSGDVITEEGSQWYEPEWWKFGDDKSYFRHATGSLVILSKNLAQYVNINSGLLKTYAFDDTTIGSWMIGVQATYIDDNRLCCSSTRQEKVCSMA
InterPro domains: - IPR025298: Domain of unknown function DUF4094 (domain) [25-102]
- IPR002659: Glycosyl transferase, family 31 (family) [93-327]
Interaction partners: - Hydroxyproline O-galactosyltransferase GALT5
- Hydroxyproline O-galactosyltransferase GALT6
- Hydroxyproline O-galactosyltransferase GALT2
- Hydroxyproline O-galactosyltransferase GALT4
- Beta-glucuronosyltransferase GlcAT14B
- Beta-arabinofuranosyltransferase RAY1
- Beta-glucuronosyltransferase GlcAT14C
- Beta-glucuronosyltransferase GlcAT14A
- Beta-1,3-galactosyltransferase GALT1
- Beta-1,6-galactosyltransferase GALT29A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016757 (glycosyltransferase activity), GO:0016758 (hexosyltransferase activity), GO:0008378 (galactosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071554 (cell wall organization or biogenesis), GO:0006807 (nitrogen compound metabolic process), GO:0070085 (glycosylation), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044036 (cell wall macromolecule metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0019538 (protein metabolic process), GO:0044249 (cellular biosynthetic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0043413 (macromolecule glycosylation), GO:0036211 (protein modification process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0006486 (protein glycosylation), GO:0043412 (macromolecule modification), GO:0009100 (glycoprotein metabolic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0006029 (proteoglycan metabolic process), GO:0009101 (glycoprotein biosynthetic process), GO:0006493 (protein O-linked glycosylation), GO:0018193 (peptidyl-amino acid modification), GO:0018208 (peptidyl-proline modification)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031984 (organelle subcompartment), GO:0005794 (Golgi apparatus), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043227 (membrane-bounded organelle), GO:0098791 (Golgi apparatus subcompartment), GO:0031985 (Golgi cisterna), GO:0043231 (intracellular membrane-bounded organelle), GO:0005795 (Golgi stack), GO:0031982 (vesicle), GO:0005802 (trans-Golgi network), GO:0005797 (Golgi medial cisterna), GO:0097708 (intracellular vesicle)