Protein: mRNA interferase toxin HigB (EC 3.1.-.-) (Endoribonuclease HigB) (Toxin HigB) (P64578)
Organism: Escherichia coli (strain K12)
Function: Toxic component of a type II toxin-antitoxin (TA) system. A probable translation-dependent mRNA interferase. Overexpression causes cessation of cell growth and inhibits cell proliferation via inhibition of translation; this blockage is overcome by subsequent expression of antitoxin HigA. Overexpression causes cleavage of a number of mRNAs in a translation-dependent fashion, suggesting this is an mRNA interferase.
Sequence: MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKGKK
InterPro domains: - IPR018669: Toxin-antitoxin system, mRNA interferase HigB (family) [20-92]
Interaction partners: - Antitoxin HigA
- Endoribonuclease toxin MazF
- mRNA interferase toxin YafQ
- mRNA interferase toxin RelE
- Toxin YoeB
- mRNA interferase toxin MqsR
- Probable mRNA interferase toxin HicA
- Antitoxin YefM
- Antitoxin HicB
- Antitoxin RelB
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0044877 (protein-containing complex binding), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0140098 (catalytic activity, acting on RNA), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0043021 (ribonucleoprotein complex binding), GO:0043023 (ribosomal large subunit binding), GO:0004518 (nuclease activity), GO:0004540 (ribonuclease activity), GO:0004521 (RNA endonuclease activity), GO:0004519 (endonuclease activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0051703 (biological process involved in intraspecies interaction between organisms), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009628 (response to abiotic stimulus), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0044010 (single-species biofilm formation), GO:0006807 (nitrogen compound metabolic process), GO:0098743 (cell aggregation), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0009892 (negative regulation of metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0048523 (negative regulation of cellular process), GO:0044238 (primary metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0044248 (cellular catabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0098630 (aggregation of unicellular organisms), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0033554 (cellular response to stress), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0034655 (nucleobase-containing compound catabolic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0006446 (regulation of translational initiation), GO:0019439 (aromatic compound catabolic process), GO:0051248 (negative regulation of protein metabolic process), GO:0046700 (heterocycle catabolic process), GO:0010468 (regulation of gene expression), GO:0009057 (macromolecule catabolic process), GO:0034248 (regulation of amide metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0044265 (cellular macromolecule catabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0042710 (biofilm formation), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0010629 (negative regulation of gene expression), GO:0034249 (negative regulation of amide metabolic process), GO:0017148 (negative regulation of translation), GO:0016070 (RNA metabolic process), GO:0016441 (post-transcriptional gene silencing), GO:0006402 (mRNA catabolic process), GO:0006401 (RNA catabolic process), GO:2000112 (regulation of cellular macromolecule biosynthetic process), GO:0006417 (regulation of translation), GO:2000113 (negative regulation of cellular macromolecule biosynthetic process), GO:0010608 (post-transcriptional regulation of gene expression), GO:0016071 (mRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0005667 (transcription regulator complex), GO:0017053 (transcription repressor complex), GO:0110001 (toxin-antitoxin complex)