Protein: Glutathione synthetase (GSH synthetase) (GSH-S) (EC 6.3.2.3) (Glutathione synthase) (P48637)
Organism: Homo sapiens (Human)
Function: Catalyzes the production of glutathione from gamma-glutamylcysteine and glycine in an ATP-dependent manner. Glutathione (gamma-glutamylcysteinylglycine, GSH) is the most abundant intracellular thiol in living aerobic cells and is required for numerous processes including the protection of cells against oxidative damage, amino acid transport, the detoxification of foreign compounds, the maintenance of protein sulfhydryl groups in a reduced state and acts as a cofactor for a number of enzymes. Participates in ophthalmate biosynthesis in hepatocytes (By similarity).
Sequence: MATNWGSLLQDKQQLEELARQAVDRALAEGVLLRTSQEPTSSEVVSYAPFTLFPSLVPSALLEQAYAVQMDFNLLVDAVSQNAAFLEQTLSSTIKQDDFTARLFDIHKQVLKEGIAQTVFLGLNRSDYMFQRSADGSPALKQIEINTISASFGGLASRTPAVHRHVLSVLSKTKEAGKILSNNPSKGLALGIAKAWELYGSPNALVLLIAQEKERNIFDQRAIENELLARNIHVIRRTFEDISEKGSLDQDRRLFVDGQEIAVVYFRDGYMPRQYSLQNWEARLLLERSHAAKCPDIATQLAGTKKVQQELSRPGMLEMLLPGQPEAVARLRATFAGLYSLDVGEEGDQAIAEALAAPSRFVLKPQREGGGNNLYGEEMVQALKQLKDSEERASYILMEKIEPEPFENCLLRPGSPARVVQCISELGIFGVYVRQEKTLVMNKHVGHLLRTKAIEHADGGVAAGVAVLDNPYPV
InterPro domains: - IPR014049: Glutathione synthase, N-terminal, eukaryotic (homologous_superfamily) [14-417]
- IPR016185: Pre-ATP-grasp domain superfamily (homologous_superfamily) [203-303]
- IPR005615: Glutathione synthase (family) [3-474]
- IPR014709: Glutathione synthase, C-terminal, eukaryotic (homologous_superfamily) [338-405]
- IPR004887: Glutathione synthase, substrate-binding domain (domain) [205-302]
- IPR014042: Glutathione synthase, alpha-helical (homologous_superfamily) [68-337]
- IPR037013: Glutathione synthase, substrate-binding domain superfamily (homologous_superfamily) [182-303]
Interaction partners: - Glutamate--cysteine ligase catalytic subunit
- Glutamate--cysteine ligase regulatory subunit
- Glutathione hydrolase 6
- Glutathione reductase, mitochondrial
- Glutathione hydrolase 1 proenzyme
- Glutathione hydrolase 7
- Glutathione hydrolase 5 proenzyme
- Hematopoietic prostaglandin D synthase
- Glutathione S-transferase omega-1
- Glutathione peroxidase 3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0005515 (protein binding), GO:0016879 (ligase activity, forming carbon-nitrogen bonds), GO:0016881 (acid-amino acid ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006749 (glutathione metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006750 (glutathione biosynthetic process), GO:0006518 (peptide metabolic process), GO:0043043 (peptide biosynthetic process), GO:0019184 (nonribosomal peptide biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)