Protein: Ribosome rescue factor SmrB (P0A8B2)
Organism: Escherichia coli (strain K12)
Function: Acts as a ribosome collision sensor. Detects stalled/collided disomes (pairs of ribosomes where the leading ribosome is stalled and a second ribosome has collided with it) and endonucleolytically cleaves mRNA at the 5' boundary of the stalled ribosome. Stalled/collided disomes form a new interface (primarily via the 30S subunits) that binds SmrB. Cleaved mRNA becomes available for tmRNA ligation, leading to ribosomal subunit dissociation and rescue of stalled ribosomes
Sequence: MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPVKRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHGLTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPKEYGGDAALLVLIEVEEWLPPELP
InterPro domains: - IPR036063: Smr domain superfamily (homologous_superfamily) [49-176]
- IPR002625: Smr domain (domain) [95-173]
- IPR022990: Ribosome rescue factor SmrB (family) [1-178]
Interaction partners: - Probable DNA endonuclease SmrA
- Small ribosomal subunit protein uS10
- Small ribosomal subunit protein uS14
- Large ribosomal subunit protein uL4
- Small ribosomal subunit protein bS21
- Small ribosomal subunit protein bS21
- Small ribosomal subunit protein bS21
- Small ribosomal subunit protein bS21
- Small ribosomal subunit protein bS21
- Small ribosomal subunit protein bS21
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:0003676 (nucleic acid binding), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0140097 (catalytic activity, acting on DNA), GO:0004518 (nuclease activity), GO:0003677 (DNA binding), GO:0004536 (deoxyribonuclease activity), GO:0004520 (DNA endonuclease activity), GO:0004519 (endonuclease activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0010467 (gene expression), GO:0018130 (heterocycle biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0016070 (RNA metabolic process), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006351 (DNA-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)