Protein: Small G protein signaling modulator 2 (RUN and TBC1 domain-containing protein 1) (O43147)
Organism: Homo sapiens (Human)
Function: Possesses GTPase activator activity towards RAB32, RAB33B and RAB38. Regulates the trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes by inactivating RAB32 and RAB38. Inhibits RAB32 and RAB38 activation both directly by promoting their GTPase activity and indirectly by disrupting the RAB9A-HPS4 interaction which is required for RAB32/38 activation.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:26620560}. Melanosome {ECO:0000269|PubMed:26620560}. Note=Melanosomal localization is mediated by RAB9A. {ECO:0000269|PubMed:26620560}.
Sequence: MGSAEDAVKEKLLWNVKKEVKQIMEEAVTRKFVHEDSSHIIALCGAVEACLLHQLRRRAAGFLRSDKMAALFTKVGKTCPVAGEICHKVQELQQQAEGRKPSGVSQEALRRQGSASGKAPALSPQALKHVWVRTALIEKVLDKVVQYLAENCSKYYEKEALLADPVFGPILASLLVGPCALEYTKLKTADHYWTDPSADELVQRHRIRGPPTRQDSPAKRPALGIRKRHSSGSASEDRLAACARECVESLHQNSRTRLLYGKNHVLVQPKEDMEAVPGYLSLHQSAESLTLKWTPNQLMNGTLGDSELEKSVYWDYALVVPFSQVVCIHCHQQKSGGTLVLVSQDGIQRPPLHFPQGGHLLSFLSCLENGLLPRGQLEPPLWTQQGKGKVFPKLRKRSSIRSVDMEEMGTGRATDYVFRIIYPGHRHEHNAGDMIEMQGFGPSLPAWHLEPLCSQGSSCLSCSSSSSPHATPSHCSCIPDRLPLRLLCESMKRQIVSRAFYGWLAHCRHLSTVRTHLSALVHHSVIPPDRPPGASAGLTKDVWSKYQKDKKNYKELELLRQVYYGGIEHEIRKDVWPFLLGHYKFGMSKKEMEQVDAVVAARYQQVLAEWKACEVVVRQREREAHPATRTKFSSGSSIDSHVQRLIHRDSTISNDVFISVDDLEPPEPQDPEDSRPKPEQEAGPGTPGTAVVEQQHSVEFDSPDSGLPSSRNYSVASGIQSSLDEGQSVGFEEEDGGGEEGSSGPGPAAHTLREPQDPSQEKPQAGELEAGEELAAVCAAAYTIELLDTVALNLHRIDKDVQRCDRNYWYFTPPNLERLRDVMCSYVWEHLDVGYVQGMCDLLAPLLVTLDNDQLAYSCFSHLMKRMSQNFPNGGAMDTHFANMRSLIQILDSELFELMHQNGDYTHFYFCYRWFLLDFKRELLYEDVFAVWEVIWAARHISSEHFVLFIALALVEAYREIIRDNNMDFTDIIKFFNERAEHHDAQEILRIARDLVHKVQMLIENK
InterPro domains: - IPR004012: RUN domain (domain) [34-191]
- IPR021935: Small G protein signalling modulator 1/2, Rab-binding domain (domain) [257-392]
- IPR047345: Small G protein signaling modulator 2, RUN domain (domain) [18-197]
- IPR035969: Rab-GAP-TBC domain superfamily (homologous_superfamily) [542-998]
- IPR000195: Rab-GAP-TBC domain (domain) [563-966]
- IPR037745: Small G protein signalling modulator 1/2, PH domain (domain) [256-424]
- IPR037213: RUN domain superfamily (homologous_superfamily) [3-200]
Interaction partners: - Small G protein signaling modulator 3
- Ras-related protein Rab-9A
- Ras-related protein Rap-1b
- Ras-related protein Rab-4A
- Ras-related protein Rab-3A
- Ras-related protein Rap-2a
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0005488 (binding), GO:0030234 (enzyme regulator activity), GO:0140677 (molecular function activator activity), GO:0005515 (protein binding), GO:0019899 (enzyme binding), GO:0008047 (enzyme activator activity), GO:0060589 (nucleoside-triphosphatase regulator activity), GO:0051020 (GTPase binding), GO:0030695 (GTPase regulator activity), GO:0005096 (GTPase activator activity), GO:0031267 (small GTPase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0051641 (cellular localization), GO:0044237 (cellular metabolic process), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0051234 (establishment of localization), GO:0009893 (positive regulation of metabolic process), GO:0051716 (cellular response to stimulus), GO:0071840 (cellular component organization or biogenesis), GO:0065009 (regulation of molecular function), GO:0061919 (process utilizing autophagic mechanism), GO:0009056 (catabolic process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0048522 (positive regulation of cellular process), GO:0050790 (regulation of catalytic activity), GO:0044093 (positive regulation of molecular function), GO:0044085 (cellular component biogenesis), GO:0035556 (intracellular signal transduction), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0044248 (cellular catabolic process), GO:0006810 (transport), GO:0060255 (regulation of macromolecule metabolic process), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0006914 (autophagy), GO:0016236 (macroautophagy), GO:0030030 (cell projection organization), GO:0051336 (regulation of hydrolase activity), GO:0016192 (vesicle-mediated transport), GO:0007264 (small GTPase mediated signal transduction), GO:0043085 (positive regulation of catalytic activity), GO:0006996 (organelle organization), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0043087 (regulation of GTPase activity), GO:0007265 (Ras protein signal transduction), GO:0007033 (vacuole organization), GO:0030031 (cell projection assembly), GO:0120036 (plasma membrane bounded cell projection organization), GO:0044782 (cilium organization), GO:0120031 (plasma membrane bounded cell projection assembly), GO:0097581 (lamellipodium organization), GO:0120031 (plasma membrane bounded cell projection assembly), GO:0060271 (cilium assembly), GO:0030032 (lamellipodium assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0031090 (organelle membrane), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0030659 (cytoplasmic vesicle membrane), GO:0012506 (vesicle membrane), GO:0097708 (intracellular vesicle)