Protein: UDP-sugar pyrophosphorylase (AtUSP) (EC 2.7.7.64) (Q9C5I1)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Required for the synthesis of the intine, the pectocellulosic inner wall of developing pollen. May function as the terminal enzyme of the myo-inositol oxidation (MIO) pathway. May also play a role in the salvage pathway for synthesis of nucleotide sugars. Can use a wide range of substrates including glucose-1-phosphate, galactose-1-phosphate, xylose-1-phosphate, arabinose-1-phosphate and glucuronate-1-phosphate.
Sequence: MASTVDSNFFSSVPALHSNLGLLSPDQIELAKILLENGQSHLFQQWPELGVDDKEKLAFFDQIARLNSSYPGGLAAYIKTAKELLADSKVGKNPYDGFSPSVPSGENLTFGTDNFIEMEKRGVVEARNAAFVLVAGGLGERLGYNGIKVALPRETTTGTCFLQHYIESILALQEASNKIDSDGSERDIPFIIMTSDDTHSRTLDLLELNSYFGMKPTQVHLLKQEKVACLDDNDARLALDPHNKYSIQTKPHGHGDVHSLLYSSGLLHKWLEAGLKWVLFFQDTNGLLFNAIPASLGVSATKQYHVNSLAVPRKAKEAIGGISKLTHVDGRSMVINVEYNQLDPLLRASGFPDGDVNCETGFSPFPGNINQLILELGPYKDELQKTGGAIKEFVNPKYKDSTKTAFKSSTRLECMMQDYPKTLPPTARVGFTVMDIWLAYAPVKNNPEDAAKVPKGNPYHSATSGEMAIYRANSLILQKAGVKVEEPVKQVLNGQEVEVWSRITWKPKWGMIFSDIKKKVSGNCEVSQRSTMAIKGRNVFIKDLSLDGALIVDSIDDAEVKLGGLIKNNGWTMESVDYKDTSVPEEIRIRGFRFNKVEQLEKKLTQPGKFSVED
InterPro domains: - IPR029044: Nucleotide-diphospho-sugar transferases (homologous_superfamily) [25-554]
- IPR002618: UDPGP family (family) [127-278]
- IPR039741: UDP-sugar pyrophosphorylase (family) [17-588]
Interaction partners: - Probable glucuronokinase 2
- Glucuronokinase 1
- Probable phosphoglucomutase, cytoplasmic 1
- UDP-arabinose 4-epimerase 1
- Probable phosphoglucomutase, cytoplasmic 2
- Phosphoglucomutase, chloroplastic
- Putative UDP-glucose 6-dehydrogenase
- Bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 3
- UTP--glucose-1-phosphate uridylyltransferase 1
- Bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016779 (nucleotidyltransferase activity), GO:0070569 (uridylyltransferase activity), GO:0051748 (UTP-monosaccharide-1-phosphate uridylyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071554 (cell wall organization or biogenesis), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0099402 (plant organ development), GO:0044036 (cell wall macromolecule metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044085 (cellular component biogenesis), GO:0042546 (cell wall biogenesis), GO:0048731 (system development), GO:0044249 (cellular biosynthetic process), GO:0016043 (cellular component organization), GO:0071669 (plant-type cell wall organization or biogenesis), GO:0005975 (carbohydrate metabolic process), GO:0071555 (cell wall organization), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0048367 (shoot system development), GO:0009059 (macromolecule biosynthetic process), GO:0042545 (cell wall modification), GO:0009664 (plant-type cell wall organization), GO:0070589 (cellular component macromolecule biosynthetic process), GO:0016051 (carbohydrate biosynthetic process), GO:0005976 (polysaccharide metabolic process), GO:0034637 (cellular carbohydrate biosynthetic process), GO:0010383 (cell wall polysaccharide metabolic process), GO:0048827 (phyllome development), GO:0034645 (cellular macromolecule biosynthetic process), GO:0045229 (external encapsulating structure organization), GO:0009832 (plant-type cell wall biogenesis), GO:0044038 (cell wall macromolecule biosynthetic process), GO:0044264 (cellular polysaccharide metabolic process), GO:0052546 (cell wall pectin metabolic process), GO:0033692 (cellular polysaccharide biosynthetic process), GO:0010393 (galacturonan metabolic process), GO:0000271 (polysaccharide biosynthetic process), GO:0070592 (cell wall polysaccharide biosynthetic process), GO:0045488 (pectin metabolic process), GO:0045489 (pectin biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0042579 (microbody), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005777 (peroxisome)