Protein: Ubiquitin-conjugating enzyme E2 B (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme B) (RAD6 homolog B) (HR6B) (hHR6B) (Ubiquitin carrier protein B) (Ubiquitin-conjugating enzyme E2-17 kDa) (Ubiquitin-protein ligase B) (P63146)
Organism: Homo sapiens (Human)
Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
Subcellular location: Cell membrane {ECO:0000250|UniProtKB:P63149}. Nucleus {ECO:0000250|UniProtKB:P63149}. Note=In peripheral neurons, expressed both at the plasma membrane and in nuclei. {ECO:0000250|UniProtKB:P63149}.
Sequence: MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS
InterPro domains: - IPR023313: Ubiquitin-conjugating enzyme, active site (active_site) [77-92]
- IPR050113: Ubiquitin-conjugating enzyme (family) [3-149]
- IPR016135: Ubiquitin-conjugating enzyme/RWD-like (homologous_superfamily) [1-152]
- IPR000608: Ubiquitin-conjugating (UBC), catalytic core domain (domain) [4-150]
Interaction partners: - E3 ubiquitin-protein ligase RAD18
- E3 ubiquitin-protein ligase BRE1A
- E3 ubiquitin-protein ligase BRE1B
- Ubiquitin-like modifier-activating enzyme 1
- E3 ubiquitin-protein ligase UBR1
- Ubiquitin conjugating enzyme E2 V1
- Ubiquitin-conjugating enzyme E2 A
- E3 ubiquitin-protein ligase UBR2
- Ubiquitin-conjugating enzyme E2 variant 2
- Ubiquitin-ribosomal protein eS31 fusion protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0032182 (ubiquitin-like protein binding), GO:0019787 (ubiquitin-like protein transferase activity), GO:0019899 (enzyme binding), GO:0004842 (ubiquitin-protein transferase activity), GO:0044389 (ubiquitin-like protein ligase binding), GO:0061650 (ubiquitin-like protein conjugating enzyme activity), GO:0043130 (ubiquitin binding), GO:0031625 (ubiquitin protein ligase binding), GO:0061631 (ubiquitin conjugating enzyme activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0009628 (response to abiotic stimulus), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:1901564 (organonitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0042127 (regulation of cell population proliferation), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0009314 (response to radiation), GO:0033554 (cellular response to stress), GO:0019538 (protein metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0008284 (positive regulation of cell population proliferation), GO:0036211 (protein modification process), GO:0009416 (response to light stimulus), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0006259 (DNA metabolic process), GO:0009411 (response to UV), GO:0070647 (protein modification by small protein conjugation or removal), GO:0006281 (DNA repair), GO:0016570 (histone modification), GO:0032446 (protein modification by small protein conjugation), GO:0016574 (histone ubiquitination), GO:0033522 (histone H2A ubiquitination), GO:0016567 (protein ubiquitination), GO:0051865 (protein autoubiquitination), GO:0000209 (protein polyubiquitination), GO:0070936 (protein K48-linked ubiquitination), GO:0070979 (protein K11-linked ubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000785 (chromatin), GO:0005829 (cytosol), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0140535 (intracellular protein-containing complex), GO:0043233 (organelle lumen), GO:1990234 (transferase complex), GO:0000151 (ubiquitin ligase complex), GO:0031371 (ubiquitin conjugating enzyme complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005694 (chromosome), GO:0005634 (nucleus)