Protein: E3 ubiquitin-protein ligase DMA1 (EC 2.3.2.27) (Checkpoint forkhead associated with RING domains-containing protein 1) (Defective in mitotic arrest protein 1) (RING-type E3 ubiquitin transferase DMA1) (P38823)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: E3 ubiquitin-protein ligase which functions in cell cycle retarding in conjunction with the UBC4 and UBC13/MMS2 complex, 2 E2 ubiquitin conjugating enzymes. Involved in nutritional control of the cell cycle. Targets the G1 cyclin PCL1 for destruction. Required for proper spindle positioning, likely regulating septin ring deposition at the bud neck.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:14562095}.
Sequence: MSTNTVPSSPPNQTPPAASGIATSHDHTKFNNPIRLPISISLTINDTPNNNSNNNSVSNGLGILPSRTATSLVVANNGSANGNVGATAAAAATVETNTAPAVNTTKSIRHFIYPPNQVNQTEFSLDIHLPPNTSLPERIDQSTLKRRMDKHGLFSIRLTPFIDTSSTSVANQGLFFDPIIRTAGAGSQIIIGRYTERVREAISKIPDQYHPVVFKSKVISRTHGCFKVDDQGNWFLKDVKSSSGTFLNHQRLSSASTTSKDYLLHDGDIIQLGMDFRGGTEEIYRCVKMKIELNKSWKLKANAFNKEALSRIKNLQKLTTGLEQEDCSICLNKIKPCQAIFISPCAHSWHFHCVRRLVIMNYPQFMCPNCRTNCDLETTLESESESEFENEDEDEPDIEMDIDMEINNNLGVRLVD
InterPro domains: - IPR000253: Forkhead-associated (FHA) domain (domain) [188-273]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [306-379]
- IPR008984: SMAD/FHA domain superfamily (homologous_superfamily) [186-298]
- IPR042823: E3 ubiquitin-protein ligase Dma1/Dma2, RING finger, H2 subclass (domain) [325-371]
- IPR001841: Zinc finger, RING-type (domain) [326-371]
Interaction partners: - E3 ubiquitin-protein ligase DMA2
- Translation initiation factor eIF2 assembly protein
- 2-oxoglutarate dehydrogenase, mitochondrial
- Protein BNI1
- Mitotic check point protein BUB2
- Mitosis inhibitor protein kinase SWE1
- Ubiquitin-conjugating enzyme E2 13
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0019787 (ubiquitin-like protein transferase activity), GO:0004842 (ubiquitin-protein transferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0051641 (cellular localization), GO:0007163 (establishment or maintenance of cell polarity), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:0051234 (establishment of localization), GO:0051301 (cell division), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0033036 (macromolecule localization), GO:0051640 (organelle localization), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0007017 (microtubule-based process), GO:0048523 (negative regulation of cellular process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0032506 (cytokinetic process), GO:0000910 (cytokinesis), GO:0044085 (cellular component biogenesis), GO:1901564 (organonitrogen compound metabolic process), GO:1903047 (mitotic cell cycle process), GO:0030010 (establishment of cell polarity), GO:0035556 (intracellular signal transduction), GO:0044248 (cellular catabolic process), GO:0019538 (protein metabolic process), GO:0051649 (establishment of localization in cell), GO:0000226 (microtubule cytoskeleton organization), GO:0010948 (negative regulation of cell cycle process), GO:0043170 (macromolecule metabolic process), GO:0044087 (regulation of cellular component biogenesis), GO:0051726 (regulation of cell cycle), GO:0051653 (spindle localization), GO:0051656 (establishment of organelle localization), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0045786 (negative regulation of cell cycle), GO:1901575 (organic substance catabolic process), GO:0051128 (regulation of cellular component organization), GO:0044260 (cellular macromolecule metabolic process), GO:0010564 (regulation of cell cycle process), GO:0000278 (mitotic cell cycle), GO:1902850 (microtubule cytoskeleton organization involved in mitosis), GO:0007346 (regulation of mitotic cell cycle), GO:1901565 (organonitrogen compound catabolic process), GO:0000075 (cell cycle checkpoint signaling), GO:0006508 (proteolysis), GO:0030163 (protein catabolic process), GO:0032956 (regulation of actin cytoskeleton organization), GO:0007093 (mitotic cell cycle checkpoint signaling), GO:0051294 (establishment of spindle orientation), GO:0022607 (cellular component assembly), GO:0036211 (protein modification process), GO:0009057 (macromolecule catabolic process), GO:1902903 (regulation of supramolecular fiber organization), GO:0043412 (macromolecule modification), GO:0061640 (cytoskeleton-dependent cytokinesis), GO:0032231 (regulation of actin filament bundle assembly), GO:0044265 (cellular macromolecule catabolic process), GO:0051293 (establishment of spindle localization), GO:1901987 (regulation of cell cycle phase transition), GO:0045930 (negative regulation of mitotic cell cycle), GO:0033043 (regulation of organelle organization), GO:0000281 (mitotic cytokinesis), GO:0043933 (protein-containing complex organization), GO:0008104 (protein localization), GO:0006996 (organelle organization), GO:1901988 (negative regulation of cell cycle phase transition), GO:0070925 (organelle assembly), GO:0051493 (regulation of cytoskeleton organization), GO:0040001 (establishment of mitotic spindle localization), GO:1901991 (negative regulation of mitotic cell cycle phase transition), GO:0065003 (protein-containing complex assembly), GO:0070647 (protein modification by small protein conjugation or removal), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0051603 (proteolysis involved in protein catabolic process), GO:0007010 (cytoskeleton organization), GO:0031577 (spindle checkpoint signaling), GO:1901990 (regulation of mitotic cell cycle phase transition), GO:0110053 (regulation of actin filament organization), GO:0000132 (establishment of mitotic spindle orientation), GO:0071174 (mitotic spindle checkpoint signaling), GO:0007096 (regulation of exit from mitosis), GO:0001100 (negative regulation of exit from mitosis), GO:0032185 (septin cytoskeleton organization), GO:0000921 (septin ring assembly), GO:0140694 (non-membrane-bounded organelle assembly), GO:0032446 (protein modification by small protein conjugation), GO:0019941 (modification-dependent protein catabolic process), GO:0031106 (septin ring organization), GO:0006511 (ubiquitin-dependent protein catabolic process), GO:0016567 (protein ubiquitination), GO:0051865 (protein autoubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0032161 (cleavage apparatus septin structure), GO:0032153 (cell division site), GO:0000399 (cellular bud neck septin structure), GO:0030427 (site of polarized growth), GO:0005933 (cellular bud), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0005934 (cellular bud tip), GO:0043229 (intracellular organelle), GO:0005938 (cell cortex), GO:0005935 (cellular bud neck), GO:0043228 (non-membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0032156 (septin cytoskeleton)