Protein: Genome polyprotein [Cleaved into: P1; Capsid protein VP0 (VP4-VP2); Capsid protein VP4 (P1A) (Virion protein 4); Capsid protein VP2 (P1B) (Virion protein 2); Capsid protein VP3 (P1C) (Virion protein 3); Capsid protein VP1 (P1D) (Virion protein 1); P2; Protease 2A (P2A) (EC 3.4.22.29) (Picornain 2A) (Protein 2A); Protein 2B (P2B); Protein 2C (P2C) (EC 3.6.1.15); P3; Protein 3AB; Protein 3A (P3A); Viral protein genome-linked (VPg) (Protein 3B) (P3B); Protein 3CD (EC 3.4.22.28); Protease 3C (EC 3.4.22.28) (Picornain 3C) (P3C); RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48) (3D polymerase) (3Dpol) (Protein 3D) (3D)] (P08292)
Organism: Coxsackievirus B4 (strain JVB / Benschoten / New York/51)
Function: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3 (By similarity). The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome (By similarity). Capsid protein VP1 mainly forms the vertices of the capsid (By similarity). Capsid protein VP1 interacts with host CXADR to provide virion attachment to target host cells (Probable). This attachment induces virion internalization (By similarity). Tyrosine kinases are probably involved in the entry process (By similarity). After binding to its receptor, the capsid undergoes conformational changes (By similarity). Capsid protein VP1 N-terminus (that contains an amphipathic alpha-helix) and capsid protein VP4 are externalized (By similarity). Together, they shape a pore in the host membrane through which viral genome is translocated to host cell cytoplasm (By similarity).
Subcellular location: [Capsid protein VP0]: Virion. Host cytoplasm {ECO:0000305}.; SUBCELLULAR LOCATION: [Capsid protein VP4]: Virion.; SUBCELLULAR LOCATION: [Capsid protein VP2]: Virion {ECO:0000250|UniProtKB:P03300}. Host cytoplasm {ECO:0000305}.; SUBCELLULAR LOCATION: [Capsid protein VP3]: Virion {ECO:0000250|UniProtKB:P03300}. Host cytoplasm {ECO:0000305}.; SUBCELLULAR LOCATION: [Capsid protein VP1]: Virion {ECO:0000250|UniProtKB:P03300}. Host cytoplasm {ECO:0000305}.; SUBCELLULAR LOCATION: [Protein 2B]: Host cytoplasmic vesicle membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum.; SUBCELLULAR LOCATION: [Protein 2C]: Host cytoplasmic vesicle membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum.; SUBCELLULAR LOCATION: [Protein 3A]: Host cytoplasmic vesicle membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum.; SUBCELLULAR LOCATION: [Protein 3AB]: Host cytoplasmic vesicle membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum.; SUBCELLULAR LOCATION: [Viral protein genome-linked]: Virion {ECO:0000250|UniProtKB:P03300}. Host cytoplasm {ECO:0000250|UniProtKB:Q66478}.; SUBCELLULAR LOCATION: [Protease 3C]: Host cytoplasm.; SUBCELLULAR LOCATION: [Protein 3CD]: Host nucleus {ECO:0000250|UniProtKB:P03300}. Host cytoplasm {ECO:0000250|UniProtKB:P03300}. Host cytoplasmic vesicle membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum.; SUBCELLULAR LOCATION: [RNA-directed RNA polymerase]: Host cytoplasmic vesicle membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum.
Sequence: MGAQVSTQKTGAHETSLSASGNSIIHYTNINYYKDAASNSANRQDFTQDPSKFTEPVKDVMIKSLPALNSPTVEECGYSDRVRSITLGNSTITTQECANVVVGYGVWPDYLSDEEATAEDQPTQPDVATCRFYTLNSVKWEMQSAGWWWKFPDALSEMGLFGQNMQYHYLGRSGYTIHVQCNASKFHQGCLLVVCVPEAEMGCTNAENAPAYGDLCGGETAKSFEQNAATGKTAVQTAVCNAGMGVGVGNLTIYPHQWINLRTNNSATIVMPYINSVPMDNMFRHNNFTLMIIPFAPLDYVTGASSYIPITVTVAPMSAEYNGLRLAGHQGLPTMLTPGSTQFLTSDDFQSPSAMPQFDVTPEMNIPGQVRNLMEIAEVDSVVPINNLKANLMTMEAYRVQVRSTDEMGGQIFGFPLQPGASSVLQRTLLGEILNYYTHWSGSLKLTFVFCGSAMATGKFLLAYSPPGAGAPDSRKNAMLGTHVIWDVGLQSSCVLCVPWISQTHYRYVVDDKYTASGFISCWYQTNVIVPAEAQKSCYIMCFVSACNDFSVRMLRDTQFIKQTNFYQGPTEESVERAMGRVADTIARGPSNSEQIPALTAVETGHTSQVDPSDTMQTRHVHNYHSRSESSIENFLCRSACVIYIKYSSAESNNLKRYAEWVINTRQVAQLRRKMEMFTYIRCDMELTFVITSHQEMSTATNSDVPVQTHQIMYVPPGGPVPTSVNDYVWQTSTNPSIFWTEGNAPPRMSIPFMSIGNAYTMFYDGWSNFSRDGIYGYNSLNNMGTIYARHVNDSSPGGLTSTIRIYFKPKHVKAYVPRPPRLCQYKKAKSVNFDVEAVTAERASLITTGPYGHQSGAVYVGNYKVVNRHLATHVDWQNCVWEDYNRDLLVSTTTAHGCDTIARCQCTTGVYFCASKSKHYPVSFEGPGLVEVQESEYYPKRYQSHVLLATGFSEPGDCGGILRCEHGVIGLVTMGGEGVVGFADVRDLLWLEDDAMEQGVKDYVEQLGNAFGSGFTNQICEQVNLLKESLVGQDSILEKSLKALVKIISALVIVVRNHDDLITVTATLALIGCTSSPWRWLKHKVSQYYGIPMAERQNNGWLKKFTEMTNACKGMEWIAVKIQKFIEWLKVKILPEVKEKHEFLSRLKQLPLLESQIATIEQSAPSQSDQEQLFSNVQYFAHYCRKYAPLYAAEAKRVFSLEKKMSNYIQFKSKCRIEPVCLLLHGSPGAGKSVATNLIGRSLAEKLNSSVYSLPPDPDHFDGYKQQAVVIMDDLCQNPDGKDVSLFCQMVSSVDFVPPMAALEEKGILFTSPFVLASTNAGSINAPTVSDSRALARRFHFDMNIEVISMYSQNGKINMPMSVKTCDEECCPVNFKRCCPLVCGKAIQFIDRKTQVRYSLDMLVTEMFREYNHRHSVGATLEALFQGPPVYREIKISVTPETPPPPVIADLLKSVDRQAIREYCKEKGWLVPEIDSILQIEKHVSRAFICLQALTTFVSVAGIIYIIYKLFAGFQGAYTGMPNQKPKVPTLRQAKVQGPAFEFAVAMMKRNSSTVKTEYGEFTMLGIYDRWAVLPRHAKPGPTILMNDQEVGVLDAKELIDRDGTNLELTLLKLNRNEKFRDIRGFLAKEEVEVNEAVLAINTSKFPNMYIPVGRVTDYGFLNLGGTPTKRMLMYNFPTRAGQCGGVLMSTGKVLGIHVGGNGHQGFSAGLLKHYFNDEQGEIEFIESSKDAGFPVINTPSRTKLEPSVFHHVFEGNKEPAVLRNGDPRLKVNFEEAIFFKYIGNVNTHVDEYMLEAVDHYAGQLATLDINTEPMKLEDAVYGTEGLEALDLTTSAGYPYVALGIKKRDILSKKTKDLTKLKECMDKYGLNLPMVTYVKDELRSAEKVAKGKSRLIEASSLNDSVAMRQTFGNLYKAFHLNPGIVTGSAVGCDPDVFWSKIPVMLDGHLIAFDYSGYDASLSPVWFACLKLLLEKLGYTHKETNYIDYLCNSHHLYRDKHYFVRGGMPSGCSGTSIFNSMINNIIIRTLMLKVYKGIDLDQFRMIAYGDDVIASYPWPIDASLLAEAGKDYGLIMTPADKGECFNEVTWTNVTFLKRYFRADEQYPFLVHPVMPMKDIHESIRWTKDPKNTQDHVRSLCLLAWHNGEHEYEEFIQKIRSVPVGRCLTLPAFSTLRRKWLDSF
InterPro domains: - IPR003593: AAA+ ATPase domain (domain) [1219-1350]
- IPR014838: Poliovirus 3A protein-like (domain) [1432-1488]
- IPR009003: Peptidase S1, PA clan (homologous_superfamily) [853-1722]
- IPR043502: DNA/RNA polymerase superfamily (homologous_superfamily) [1730-2182]
- IPR043128: Reverse transcriptase/Diguanylate cyclase domain (homologous_superfamily) [1990-2089]
- IPR036203: Poliovirus core protein 3a, soluble domain (homologous_superfamily) [1427-1488]
- IPR033703: Picornavirus/Calicivirus coat protein (domain) [125-822]
- IPR036988: Picornavirus coat protein VP4 superfamily (homologous_superfamily) [1-69]
- IPR043504: Peptidase S1, PA clan, chymotrypsin-like fold (homologous_superfamily) [862-1719]
- IPR001205: RNA-directed RNA polymerase, C-terminal domain (domain) [1748-2158]
- IPR000081: Peptidase C3, picornavirus core protein 2A (domain) [868-994]
- IPR000199: Peptidase C3A/C3B, picornaviral (domain) [1539-1704]
- IPR000605: Helicase, superfamily 3, single-stranded DNA/RNA virus (domain) [1224-1321]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [1207-1360]
- IPR007094: RNA-directed RNA polymerase, catalytic domain (domain) [1948-2064]
- IPR014759: Helicase, superfamily 3, single-stranded RNA virus (domain) [1203-1359]
- IPR029053: Viral coat protein subunit (homologous_superfamily) [70-861]
- IPR001676: Picornavirus capsid (domain) [93-524]
- IPR003138: Picornavirus coat protein VP4 (family) [2-69]
- IPR002527: Picornavirus 2B protein (domain) [999-1098]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0008233 (peptidase activity), GO:0008234 (cysteine-type peptidase activity), GO:0004175 (endopeptidase activity), GO:0004197 (cysteine-type endopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0016032 (viral process), GO:0044068 (modulation by symbiont of host cellular process), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0035821 (modulation of process of another organism), GO:0019048 (modulation by virus of host process), GO:0044403 (biological process involved in symbiotic interaction), GO:0048522 (positive regulation of cellular process), GO:0009894 (regulation of catabolic process), GO:0051701 (biological process involved in interaction with host), GO:0052040 (modulation by symbiont of host programmed cell death), GO:0010941 (regulation of cell death), GO:0031325 (positive regulation of cellular metabolic process), GO:0044003 (modulation by symbiont of host process), GO:0031323 (regulation of cellular metabolic process), GO:0009896 (positive regulation of catabolic process), GO:0019054 (modulation by virus of host cellular process), GO:0010508 (positive regulation of autophagy), GO:0043067 (regulation of programmed cell death), GO:0010506 (regulation of autophagy), GO:0031331 (positive regulation of cellular catabolic process), GO:0031329 (regulation of cellular catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0018995 (host cellular component), GO:0043657 (host cell), GO:0033643 (host cell part), GO:0033646 (host intracellular part), GO:0043656 (host intracellular region), GO:0033647 (host intracellular organelle), GO:0033648 (host intracellular membrane-bounded organelle), GO:0042025 (host cell nucleus)