Protein: 3-isopropylmalate dehydrogenase 2, chloroplastic (3-IPM-DH 2) (AtIMDH2) (AtIMDH3) (IMDH 2) (EC 1.1.1.85) (Beta-IPM dehydrogenase 2) (Isopropylmalate dehydrogenase 2) (AtIMD2) (P93832)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Involved in leucine biosynthesis; catalyzes the oxidative decarboxylation step in leucine biosynthesis (primary metabolism). Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate, 3-IPM) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. Required during pollen development and involved in embryo sac development.
Subcellular location: Plastid, chloroplast stroma {ECO:0000250|UniProtKB:Q9FMT1}.
Sequence: MAAALQTNIRTVKVPATFRAVSKQSLAPFRVRCAVASPGKKRYTITLLPGDGIGPEVVSIAKNVLQQAGSLEGVEFNFREMPIGGAALDLVGVPLPEETISAAKESDAVLLGAIGGYKWDNNEKHLRPEKGLLQIRAALKVFANLRPATVLPQLVDASTLKREVAEGVDLMVVRELTGGIYFGEPRGIKTNENGEEVGFNTEVYAAHEIDRIARVAFETARKRRGKLCSVDKANVLEASILWRKRVTALASEYPDVELSHMYVDNAAMQLVRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLSDSGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEKAAKRIEDAVLVALNNGFRTGDIYSAGTKLVGCKEMGEEVLKSVDSQVPASV
InterPro domains: - IPR004429: Isopropylmalate dehydrogenase (family) [39-403]
- IPR024084: Isopropylmalate dehydrogenase-like domain (domain) [44-393]
- IPR019818: Isocitrate/isopropylmalate dehydrogenase, conserved site (conserved_site) [284-303]
Interaction partners: - 3-isopropylmalate dehydratase small subunit 1
- 3-isopropylmalate dehydratase small subunit 3
- 3-isopropylmalate dehydratase large subunit, chloroplastic
- 3-isopropylmalate dehydratase small subunit 2
- Acetolactate synthase, chloroplastic
- Acetolactate synthase small subunit 1, chloroplastic
- Acetolactate synthase small subunit 2, chloroplastic
- Threonine dehydratase biosynthetic, chloroplastic
- 2-isopropylmalate synthase 1, chloroplastic
- Methylthioalkylmalate synthase 1, chloroplastic
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016614 (oxidoreductase activity, acting on CH-OH group of donors), GO:0016616 (oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0044281 (small molecule metabolic process), GO:0009719 (response to endogenous stimulus), GO:0006807 (nitrogen compound metabolic process), GO:0006950 (response to stress), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0009611 (response to wounding), GO:0006082 (organic acid metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901700 (response to oxygen-containing compound), GO:0006520 (amino acid metabolic process), GO:0048229 (gametophyte development), GO:0006790 (sulfur compound metabolic process), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:1901135 (carbohydrate derivative metabolic process), GO:0019757 (glycosinolate metabolic process), GO:0019760 (glucosinolate metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0016143 (S-glycoside metabolic process), GO:0009555 (pollen development), GO:0044272 (sulfur compound biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0009553 (embryo sac development), GO:0033993 (response to lipid), GO:0009081 (branched-chain amino acid metabolic process), GO:1901657 (glycosyl compound metabolic process), GO:0019758 (glycosinolate biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:1901605 (alpha-amino acid metabolic process), GO:0070542 (response to fatty acid), GO:0009753 (response to jasmonic acid), GO:0006551 (leucine metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:1901659 (glycosyl compound biosynthetic process), GO:0019761 (glucosinolate biosynthetic process), GO:0009082 (branched-chain amino acid biosynthetic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0016144 (S-glycoside biosynthetic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0009098 (leucine biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0009532 (plastid stroma), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009570 (chloroplast stroma), GO:0043228 (non-membrane-bounded organelle), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0009579 (thylakoid)