Protein: CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4 (Alpha 2,3-ST 4) (Beta-galactoside alpha-2,3-sialyltransferase 4) (EC 2.4.3.2) (EC 2.4.3.4) (Alpha 2,3-sialyltransferase IV) (Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase) (Gal-beta-1,4-GlcNAc-alpha-2,3-sialyltransferase) (N-acetyllactosaminide alpha-2,3-sialyltransferase) (EC 2.4.3.6) (ST3Gal IV) (ST3GalIV) (Sialyltransferase 4C) (SIAT4-C) (Q91Y74)
Organism: Mus musculus (Mouse)
Function: A beta-galactoside alpha2-3 sialyltransferase involved in terminal sialylation of glycoproteins and glycolipids (By similarity). Catalyzes the transfer of sialic acid (N-acetyl-neuraminic acid; Neu5Ac) from the nucleotide sugar donor CMP-Neu5Ac onto acceptor Galbeta-(1->3)-GalNAc- and Galbeta-(1->4)-GlcNAc-terminated glycoconjugates through an alpha2-3 linkage (By similarity). Plays a major role in hemostasis. Responsible for sialylation of plasma VWF/von Willebrand factor, preventing its recognition by asialoglycoprotein receptors (ASGPR) and subsequent clearance. Regulates ASGPR-mediated clearance of platelets. Participates in the biosynthesis of the sialyl Lewis X epitopes, both on O- and N-glycans, which are recognized by SELE/E-selectin, SELP/P-selectin and SELL/L-selectin. Essential for selectin-mediated rolling and adhesion of leukocytes during extravasation. Contributes to adhesion and transendothelial migration of neutrophils likely through terminal sialylation of CXCR2. In glycosphingolipid biosynthesis, sialylates GM1 and GA1 gangliosides to form GD1a and GM1b, respectively (By similarity). Metabolizes brain c-series ganglioside GT1c forming GQ1c (By similarity). Synthesizes ganglioside LM1 (IV3Neu5Ac-nLc4Cer), a major structural component of peripheral nerve myelin (By similarity).
Subcellular location: Golgi apparatus, Golgi stack membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}. Note=Membrane-bound form in trans cisternae of Golgi. {ECO:0000250}.
Sequence: MTSKSHWKLLALALVLVVVMVWYSISREDRYIEFFYFPISEKKEPCFQGEAERQASKIFGNRSREQPIFLQLKDYFWVKTPSTYELPFGTKGSEDLLLRVLAITSYSIPESIKSLECRRCVVVGNGHRLRNSSLGGVINKYDVVIRLNNAPVAGYEGDVGSKTTIRLFYPESAHFDPKIENNPDTLLVLVAFKAMDFHWIETILSDKKRVRKGFWKQPPLIWDVNPKQVRILNPFFMEIAADKLLSLPIQQPRKIKQKPTTGLLAITLALHLCDLVHIAGFGYPDASNKKQTIHYYEQITLKSMAGSGHNVSQEAIAIKRMLEMGAVKNLTYF
InterPro domains: - IPR001675: Glycosyl transferase family 29 (family) [82-324]
- IPR012163: Sialyltransferase (family) [2-333]
- IPR051142: Glycosyltransferase 29 (family) [2-332]
- IPR038578: GT29-like superfamiliy (homologous_superfamily) [37-331]
Interaction partners: - Galactoside alpha-
- Galactoside alpha-
- Beta-1,3-galactosyltransferase 1
- N-acetyllactosaminide alpha-1,3-galactosyltransferase
- Beta-1,3-galactosyltransferase 5
- Galactoside 2-alpha-L-fucosyltransferase Sec1
- Beta-1,3-galactosyltransferase 2
- CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase
- Alpha-
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016757 (glycosyltransferase activity), GO:0008373 (sialyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0016032 (viral process), GO:0009058 (biosynthetic process), GO:0006807 (nitrogen compound metabolic process), GO:0070085 (glycosylation), GO:0002682 (regulation of immune system process), GO:0071704 (organic substance metabolic process), GO:0003008 (system process), GO:0044237 (cellular metabolic process), GO:0019080 (viral gene expression), GO:0050794 (regulation of cellular process), GO:0019082 (viral protein processing), GO:0044238 (primary metabolic process), GO:0040012 (regulation of locomotion), GO:0048522 (positive regulation of cellular process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:2000145 (regulation of cell motility), GO:0002685 (regulation of leukocyte migration), GO:0019538 (protein metabolic process), GO:0030155 (regulation of cell adhesion), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0045785 (positive regulation of cell adhesion), GO:0005975 (carbohydrate metabolic process), GO:0043170 (macromolecule metabolic process), GO:0043413 (macromolecule glycosylation), GO:1901135 (carbohydrate derivative metabolic process), GO:0006629 (lipid metabolic process), GO:0050877 (nervous system process), GO:0044260 (cellular macromolecule metabolic process), GO:0006790 (sulfur compound metabolic process), GO:0050890 (cognition), GO:0009059 (macromolecule biosynthetic process), GO:0022409 (positive regulation of cell-cell adhesion), GO:0022407 (regulation of cell-cell adhesion), GO:0016051 (carbohydrate biosynthetic process), GO:0030334 (regulation of cell migration), GO:0006022 (aminoglycan metabolic process), GO:0036211 (protein modification process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006486 (protein glycosylation), GO:0043412 (macromolecule modification), GO:0009311 (oligosaccharide metabolic process), GO:0009100 (glycoprotein metabolic process), GO:0044272 (sulfur compound biosynthetic process), GO:0002691 (regulation of cellular extravasation), GO:0006643 (membrane lipid metabolic process), GO:0030258 (lipid modification), GO:1903509 (liposaccharide metabolic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0046467 (membrane lipid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0009312 (oligosaccharide biosynthetic process), GO:0006493 (protein O-linked glycosylation), GO:0006664 (glycolipid metabolic process), GO:1903037 (regulation of leukocyte cell-cell adhesion), GO:1903039 (positive regulation of leukocyte cell-cell adhesion), GO:0030203 (glycosaminoglycan metabolic process), GO:0009101 (glycoprotein biosynthetic process), GO:0009247 (glycolipid biosynthetic process), GO:0006023 (aminoglycan biosynthetic process), GO:1904996 (positive regulation of leukocyte adhesion to vascular endothelial cell), GO:1904994 (regulation of leukocyte adhesion to vascular endothelial cell), GO:0006024 (glycosaminoglycan biosynthetic process), GO:0016266 (O-glycan processing), GO:1903510 (mucopolysaccharide metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0016020 (membrane), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0005794 (Golgi apparatus), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0000139 (Golgi membrane), GO:0043231 (intracellular membrane-bounded organelle)