Protein: Histone-lysine N-methyltransferase SETDB2 (EC 2.1.1.366) (SET domain bifurcated 2) (Q06ZW3)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis. During early development, required to specify the left-right axis by repressing expression of FGF8, leading to negatively regulate the dorsal organizer formation.
Subcellular location: Nucleus {ECO:0000250}. Chromosome {ECO:0000250}.
Sequence: METEADTAKTFWSEVDVDGVFDELMEVLRRLRHTLRHNTATDREYVQAMRIVQESKLIRTETEDVLLDEDILTVTVSEPQCSPESLQNGIEPEDPQHSTARLYSDVGVVCSHMSSSRPDPAAPLVFQPHVCSSACVPHLPAHTHHLLGHNPLRAPLLCHFQRVCDDAGVVYKAPCGRSLSCMQEVLHFLLQTQSVCVLQTDRFSFSTQVCVERQVCAAPLLERDLSRGLEPVPVALVNTVDGARPREFRYRRERWPHGCFLSAEPLYSVCCDCTDGCTDAHSCACVRRTAGAAYTHQRLTHTLRTGLFECGPWCGCERSRCENRVVQKGLRVRLQVFRTPEHMWAVRCRDDLDAGTFICIYAGVVLRLQQSSECPAERSGEPAVSDDEVQLVEEWRIPEETHTHTHTLDSSPPLHVPVIQRPAEHSLAQRRDQQQFSISSETEDNRCEQALRKKPRLMESNGLQDSRTHTLTHTHDGVYYLDASREGNVARFFTHSDDPNLFIQNVFTDTHDPQFPLIAFFTCRPVKAGTELTWSCTNTEQQKTEEKHCSV
InterPro domains: - IPR016177: DNA-binding domain superfamily (homologous_superfamily) [150-213]
- IPR046341: SET domain superfamily (homologous_superfamily) [206-550]
- IPR001214: SET domain (domain) [332-543]
- IPR051516: Histone-lysine N-methyltransferase SETDB (family) [1-537]
- IPR001739: Methyl-CpG DNA binding (domain) [149-217]
- IPR007728: Pre-SET domain (domain) [222-329]
Interaction partners: - SET domain and mariner transposase fusion protein.
- ADP-ribosylation factor-like 14 effector protein
- PHD finger protein 11.
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0008276 (protein methyltransferase activity), GO:0016279 (protein-lysine N-methyltransferase activity), GO:0042054 (histone methyltransferase activity), GO:0008168 (methyltransferase activity), GO:0018024 (histone lysine N-methyltransferase activity), GO:0140938 (histone H3 methyltransferase activity), GO:0008757 (S-adenosylmethionine-dependent methyltransferase activity), GO:0008170 (N-methyltransferase activity), GO:0016278 (lysine N-methyltransferase activity), GO:0042800 (histone H3K4 methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0032259 (methylation), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0043414 (macromolecule methylation), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0006479 (protein methylation), GO:0008213 (protein alkylation), GO:0018022 (peptidyl-lysine methylation), GO:0016571 (histone methylation), GO:0018193 (peptidyl-amino acid modification), GO:0016570 (histone modification), GO:0034968 (histone lysine methylation), GO:0061647 (histone H3-K9 modification), GO:0018205 (peptidyl-lysine modification), GO:0051567 (histone H3-K9 methylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)