Protein: Calpain-2 catalytic subunit (EC 3.4.22.53) (Calcium-activated neutral proteinase 2) (CANP 2) (Calpain M-type) (Calpain large polypeptide L2) (Calpain-2 large subunit) (Millimolar-calpain) (M-calpain) (P17655)
Organism: Homo sapiens (Human)
Function: Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity).
Subcellular location: Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding.
Sequence: MAGIAAKLAKDREAAEGLGSHDRAIKYLNQDYEALRNECLEAGTLFQDPSFPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSFSDFLRHYSRLEICNLTPDTLTSDTYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGESGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELSGQTNIHLSKNFFLTNRARERSDTFINLREVLNRFKLPPGEYILVPSTFEPNKDGDFCIRVFSEKKADYQAVDDEIEANLEEFDISEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARFADDQLIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIELDLISWLCFSVL
InterPro domains: - IPR022683: Peptidase C2, calpain, domain III (domain) [355-510]
- IPR001300: Peptidase C2, calpain, catalytic domain (domain) [27-352]
- IPR036213: Calpain large subunit, domain III superfamily (homologous_superfamily) [356-513]
- IPR000169: Cysteine peptidase, cysteine active site (active_site) [99-110]
- IPR033883: Calpain subdomain III (domain) [354-512]
- IPR022684: Peptidase C2, calpain family (family) [4-672]
- IPR011992: EF-hand domain pair (homologous_superfamily) [525-697]
- IPR002048: EF-hand domain (domain) [545-637]
- IPR022682: Peptidase C2, calpain, large subunit, domain III (domain) [362-502]
- IPR042736: CAPN2, penta-EF hand, calcium binding motif (domain) [533-700]
- IPR038765: Papain-like cysteine peptidase superfamily (homologous_superfamily) [4-353]
- IPR018247: EF-Hand 1, calcium-binding site (binding_site) [615-627]
Interaction partners: - Calpain small subunit 1
- Calpastatin
- Calpain-1 catalytic subunit
- Talin-1
- Talin-2
- BH3-interacting domain death agonist
- Cellular tumor antigen p53
- Bcl-2 homologous antagonist/killer
- Cyclin-dependent kinase 5 activator 1
- Apoptosis regulator BAX
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0043167 (ion binding), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0008233 (peptidase activity), GO:0043169 (cation binding), GO:0008234 (cysteine-type peptidase activity), GO:0004175 (endopeptidase activity), GO:0046872 (metal ion binding), GO:0004197 (cysteine-type endopeptidase activity), GO:0005509 (calcium ion binding), GO:0004198 (calcium-dependent cysteine-type endopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0042592 (homeostatic process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009628 (response to abiotic stimulus), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0009892 (negative regulation of metabolic process), GO:0065008 (regulation of biological quality), GO:0019725 (cellular homeostasis), GO:0008219 (cell death), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0048583 (regulation of response to stimulus), GO:0051716 (cellular response to stimulus), GO:0065009 (regulation of molecular function), GO:0006950 (response to stress), GO:0048523 (negative regulation of cellular process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0050790 (regulation of catalytic activity), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0012501 (programmed cell death), GO:1901564 (organonitrogen compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0060548 (negative regulation of cell death), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0045454 (cell redox homeostasis), GO:0006979 (response to oxidative stress), GO:0009314 (response to radiation), GO:0080134 (regulation of response to stress), GO:0019538 (protein metabolic process), GO:0080135 (regulation of cellular response to stress), GO:0060255 (regulation of macromolecule metabolic process), GO:0031647 (regulation of protein stability), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0010033 (response to organic substance), GO:0009894 (regulation of catabolic process), GO:0104004 (cellular response to environmental stimulus), GO:0010941 (regulation of cell death), GO:0031325 (positive regulation of cellular metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0071214 (cellular response to abiotic stimulus), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:0031323 (regulation of cellular metabolic process), GO:0009895 (negative regulation of catabolic process), GO:0080090 (regulation of primary metabolic process), GO:1901575 (organic substance catabolic process), GO:0008284 (positive regulation of cell population proliferation), GO:0071478 (cellular response to radiation), GO:0009416 (response to light stimulus), GO:0042177 (negative regulation of protein catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0042176 (regulation of protein catabolic process), GO:0051246 (regulation of protein metabolic process), GO:0043069 (negative regulation of programmed cell death), GO:0043067 (regulation of programmed cell death), GO:0071310 (cellular response to organic substance), GO:0062197 (cellular response to chemical stress), GO:0051248 (negative regulation of protein metabolic process), GO:0010506 (regulation of autophagy), GO:1990000 (amyloid fibril formation), GO:0010468 (regulation of gene expression), GO:0022607 (cellular component assembly), GO:0050727 (regulation of inflammatory response), GO:0036293 (response to decreased oxygen levels), GO:0006915 (apoptotic process), GO:0051247 (positive regulation of protein metabolic process), GO:0043933 (protein-containing complex organization), GO:0006996 (organelle organization), GO:0031648 (protein destabilization), GO:0022411 (cellular component disassembly), GO:0010629 (negative regulation of gene expression), GO:0051174 (regulation of phosphorus metabolic process), GO:0042981 (regulation of apoptotic process), GO:0030162 (regulation of proteolysis), GO:0031399 (regulation of protein modification process), GO:0065003 (protein-containing complex assembly), GO:0031400 (negative regulation of protein modification process), GO:1901799 (negative regulation of proteasomal protein catabolic process), GO:0043066 (negative regulation of apoptotic process), GO:0008154 (actin polymerization or depolymerization), GO:0051603 (proteolysis involved in protein catabolic process), GO:0019220 (regulation of phosphate metabolic process), GO:0071482 (cellular response to light stimulus), GO:0009411 (response to UV), GO:2000059 (negative regulation of ubiquitin-dependent protein catabolic process), GO:0061136 (regulation of proteasomal protein catabolic process), GO:0045861 (negative regulation of proteolysis), GO:2000058 (regulation of ubiquitin-dependent protein catabolic process), GO:0045936 (negative regulation of phosphate metabolic process), GO:0042325 (regulation of phosphorylation), GO:0001933 (negative regulation of protein phosphorylation), GO:0001932 (regulation of protein phosphorylation), GO:1903320 (regulation of protein modification by small protein conjugation or removal), GO:0032434 (regulation of proteasomal ubiquitin-dependent protein catabolic process), GO:0042326 (negative regulation of phosphorylation), GO:0032435 (negative regulation of proteasomal ubiquitin-dependent protein catabolic process), GO:1903051 (negative regulation of proteolysis involved in protein catabolic process), GO:1903050 (regulation of proteolysis involved in protein catabolic process), GO:0051259 (protein complex oligomerization), GO:0034644 (cellular response to UV), GO:0051260 (protein homooligomerization), GO:0031396 (regulation of protein ubiquitination), GO:0033137 (negative regulation of peptidyl-serine phosphorylation), GO:0033135 (regulation of peptidyl-serine phosphorylation), GO:0031397 (negative regulation of protein ubiquitination), GO:1904667 (negative regulation of ubiquitin protein ligase activity)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0000785 (chromatin), GO:0005829 (cytosol), GO:0071944 (cell periphery), GO:0030054 (cell junction), GO:0005615 (extracellular space), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0012505 (endomembrane system), GO:0005576 (extracellular region), GO:0099080 (supramolecular complex), GO:0036464 (cytoplasmic ribonucleoprotein granule), GO:0030141 (secretory granule), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043230 (extracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0099081 (supramolecular polymer), GO:0043233 (organelle lumen), GO:0070161 (anchoring junction), GO:0070062 (extracellular exosome), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0030055 (cell-substrate junction), GO:0101002 (ficolin-1-rich granule), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0065010 (extracellular membrane-bounded organelle), GO:0099512 (supramolecular fiber), GO:0070013 (intracellular organelle lumen), GO:0031982 (vesicle), GO:0005925 (focal adhesion), GO:1904813 (ficolin-1-rich granule lumen), GO:1903561 (extracellular vesicle), GO:0097708 (intracellular vesicle), GO:0005634 (nucleus), GO:0005856 (cytoskeleton), GO:0005694 (chromosome), GO:0015629 (actin cytoskeleton)