Protein: 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (EC 2.3.1.29) (Glycine acetyltransferase) (P0AB77)
Organism: Escherichia coli (strain K12)
Function: Catalyzes the cleavage of 2-amino-3-ketobutyrate to glycine and acetyl-CoA.
Sequence: MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHVINFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGMEDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQELEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFVGENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNSLAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGDAVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGKQLGVIA
InterPro domains: - IPR011282: 2-amino-3-ketobutyrate coenzyme A ligase (family) [2-397]
- IPR001917: Aminotransferase, class-II, pyridoxal-phosphate binding site (binding_site) [241-250]
- IPR015421: Pyridoxal phosphate-dependent transferase, major domain (homologous_superfamily) [58-296]
- IPR050087: 8-amino-7-oxononanoate synthase class-II (family) [18-389]
- IPR004839: Aminotransferase, class I/classII, large domain (domain) [43-387]
- IPR015424: Pyridoxal phosphate-dependent transferase (homologous_superfamily) [5-396]
- IPR015422: Pyridoxal phosphate-dependent transferase, small domain (homologous_superfamily) [38-388]
Interaction partners: - L-threonine 3-dehydrogenase
- Low specificity L-threonine aldolase
- Serine hydroxymethyltransferase
- Glycine dehydrogenase
- NADP-dependent 3-hydroxy acid dehydrogenase YdfG
- ATP-dependent dethiobiotin synthetase BioD 1
- Protein HtrL
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0016740 (transferase activity), GO:0043168 (anion binding), GO:0019842 (vitamin binding), GO:0016746 (acyltransferase activity), GO:0070279 (vitamin B6 binding), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups), GO:0030170 (pyridoxal phosphate binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0006082 (organic acid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0006766 (vitamin metabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006767 (water-soluble vitamin metabolic process), GO:0009110 (vitamin biosynthetic process), GO:0044272 (sulfur compound biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006768 (biotin metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0042364 (water-soluble vitamin biosynthetic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0009102 (biotin biosynthetic process), GO:0072330 (monocarboxylic acid biosynthetic process), GO:0032787 (monocarboxylic acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0005737 (cytoplasm)