Protein: Protein smoothened (P97698)
Organism: Rattus norvegicus (Rat)
Function: G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation.
Subcellular location: Cell membrane {ECO:0000250|UniProtKB:P56726}; Multi-pass membrane protein {ECO:0000255}. Cell projection, cilium {ECO:0000250|UniProtKB:P56726}. Note=Cilium localization is promoted by SHH and is required for activity. {ECO:0000250|UniProtKB:P56726}.
Sequence: MAAGRPVRGPELAPRRLLQLLLLVLLGGRGRGAALSGNVTGPGPRSAGGSARRNAPVTSPPPPLLSHCGRAAHCEPLRYNVCLGSALPYGATTTLLAGDSDSQEEAHSKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVERERGWPDFLRCTPDHFPEGCPNEVQNIKFNSSGQCEAPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSYIAAFGAVTGLCTLFTLATFVADWRNSNRYPAVILFYVNACFFVGSIGWLAQFMDGARREIVCRADGTMRFGEPTSSETLSCVIIFVIVYYALMAGVVWFVVLTYAWHTSFKALGTTYQPLSGKTSYFHLLTWSLPFVLTVAILAVAQVDGDSVSGICFVGYKNYRYRAGFVLAPIGLVLIVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGIFGFLAFGFVLITFSCHFYDFFNQAEWERSFRDYVLCQANVTIGLPTKKPIPDCEIKNRPSLLVEKINLFAMFGTGIAMSTWVWTKATLLIWRRTWCRLTGHSDDEPKRIKKSKMIAKAFSKRRELLQNPGQELSFSMHTVSHDGPVAGLAFELNEPSADVSSAWAQHVTKMVARRGAILPQDVSVTPVATPVPPEEQANLWLVEAEISPELEKRLGRKKKRRKRKKEVCPLGPAPELHHSAPVPATSAVPRLPQLPRQKCLVAANAWGTGEPCRQGAWTVVSNPFCPEPSPHQDPFLPGASAPRVWAQGRLQGLGSIHSRTNLMEAELLDADSDF
InterPro domains: - IPR035683: Smoothened, 7TM (domain) [224-554]
- IPR036790: Frizzled cysteine-rich domain superfamily (homologous_superfamily) [62-195]
- IPR041771: Smoothened, cysteine-rich domain (domain) [70-200]
- IPR015526: Frizzled/secreted frizzled-related protein (family) [66-551]
- IPR020067: Frizzled domain (domain) [69-187]
- IPR017981: GPCR, family 2-like, 7TM (domain) [235-497]
- IPR000539: Frizzled/Smoothened, 7TM (domain) [224-559]
Interaction partners: - Patched
- Sonic hedgehog protein
- Suppressor of fused homolog
- Hedgehog protein
- Patched 2
- Beta-arrestin-2
- GLI family zinc finger 1
- Beta-arrestin-1
- Kinesin-like protein
- EvC ciliary complex subunit 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0036094 (small molecule binding), GO:0097159 (organic cyclic compound binding), GO:0008289 (lipid binding), GO:0043178 (alcohol binding), GO:0005496 (steroid binding), GO:0032934 (sterol binding), GO:0015485 (cholesterol binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0009605 (response to external stimulus), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0042221 (response to chemical), GO:0051716 (cellular response to stimulus), GO:0007154 (cell communication), GO:0009991 (response to extracellular stimulus), GO:0007584 (response to nutrient), GO:0060322 (head development), GO:0070887 (cellular response to chemical stimulus), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0031668 (cellular response to extracellular stimulus), GO:0071496 (cellular response to external stimulus), GO:0007420 (brain development), GO:0007399 (nervous system development), GO:0031667 (response to nutrient levels), GO:0031670 (cellular response to nutrient), GO:0007417 (central nervous system development), GO:0014070 (response to organic cyclic compound), GO:0031669 (cellular response to nutrient levels), GO:0061008 (hepaticobiliary system development), GO:0071305 (cellular response to vitamin D), GO:0001889 (liver development), GO:0045990 (carbon catabolite regulation of transcription), GO:0071407 (cellular response to organic cyclic compound), GO:1903507 (negative regulation of nucleic acid-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0071944 (cell periphery), GO:0098862 (cluster of actin-based cell projections), GO:0098590 (plasma membrane region), GO:0120025 (plasma membrane bounded cell projection), GO:0005903 (brush border), GO:0005886 (plasma membrane), GO:0031526 (brush border membrane), GO:0031253 (cell projection membrane)