Protein: Leucine-rich repeat protein SHOC-2 (Q6AYI5)
Organism: Rattus norvegicus (Rat)
Function: Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates activation of the MAPK pathway (By similarity). Acts as a scaffolding protein in the SMP complex (By similarity). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (By similarity). The SMP complex enhances the dephosphorylation activity and substrate specificity of PP1c (By similarity)
Subcellular location: Cytoplasm. Nucleus. Note=Translocates from cytoplasm to nucleus upon growth factor stimulation
Sequence: MSSSLGKEKDSKEKDPKVPSAKEREKEAKASGGFGKESKEKEPKTKGKDAKDGKKESSAAQPGVAFSVDNTIKRPNPALGTRKKSSNAEVTKELNKCREENSMRLDLSKRSIHILPPSVKELTQLTELYLYSNKLQSLPAEVGCLVNLMTLALSENSLTSLPDSLDNLKKLRMLDLRHNKLREIPSVVYRLDSLTTLYLRFNRITAVEKDVRNLPRLSTLSIRENKIKQLPAEIGELCNLITLDVAHNQLEHLPKEIGNCTQITNLDLQHNELLDLPDTIGNLSSLNRLGLRYNRLSAIPRSLAKCSALEELNLENNNISTLPESLLSSLVKLNSLTLARNCFQLYPVGGPSQFSTIYSLNMEHNRINKIPFGIFSRAKVLSKLNMKDNQLTSLPLDFGTWTSMVELNLATNQLTKIPEDVSGLVSLEVLILSNNLLKKLPHGLGNLRKLRELDLEENKLESLPNEIAYLKDLQKLVLTNNQLTTLPRGIGHLTNLTHLGLGENLLTHLPEEIGTLENLEELYLNDNPNLHSLPFELALCSKLSIMSIENCPLSHLPPQIVAGGPSFIIQFLKMQGPYRAMV
InterPro domains: - IPR050216: Leucine-rich repeat domain-containing protein (family) [428-560]
- IPR001611: Leucine-rich repeat (repeat) [124-470]
- IPR055414: Disease resistance R13L4/SHOC-2-like, LRR domain (domain) [209-526]
- IPR003591: Leucine-rich repeat, typical subtype (repeat) [122-540]
- IPR032675: Leucine-rich repeat domain superfamily (homologous_superfamily) [36-573]
Interaction partners: - Ras-related protein M-Ras
- Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
- Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
- Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
- GTPase HRas
- GTPase NRas
- RAF proto-oncogene serine/threonine-protein kinase
- RAS related 2
- Ras-related protein R-Ras
- Serine/threonine-protein phosphatase PP1-beta catalytic subunit
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0019899 (enzyme binding), GO:0051020 (GTPase binding), GO:0031267 (small GTPase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0048583 (regulation of response to stimulus), GO:0023051 (regulation of signaling), GO:0023056 (positive regulation of signaling), GO:0048522 (positive regulation of cellular process), GO:0048584 (positive regulation of response to stimulus), GO:0050794 (regulation of cellular process), GO:0009967 (positive regulation of signal transduction), GO:0010647 (positive regulation of cell communication), GO:0010646 (regulation of cell communication), GO:0009966 (regulation of signal transduction), GO:1902533 (positive regulation of intracellular signal transduction), GO:1902531 (regulation of intracellular signal transduction), GO:0051057 (positive regulation of small GTPase mediated signal transduction), GO:0051056 (regulation of small GTPase mediated signal transduction), GO:0046579 (positive regulation of Ras protein signal transduction), GO:0046578 (regulation of Ras protein signal transduction)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0016020 (membrane), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)