Protein: Serine/threonine-protein kinase Nek6 (Q9ES70)
Organism: Mus musculus (Mouse)
Function: Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase- anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, TRIP4, RPS6KB1 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence (By similarity). Phosphorylates STAT3. Phosphorylates EML4 at 'Ser-144', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (By similarity)
Subcellular location: Cytoplasm. Nucleus. Nucleus speckle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Co- localizes with APBB1 at the nuclear speckles. Colocalizes with PIN1 in the nucleus. Colocalizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis (By similarity)
Sequence: MAGQPSHMPHGGSPNHLCHALGPAPPPDPQRLPNTLSFRCSLADFQIEKKIGRGQFSEVYKATCLLDRKTVALKKVQIFEMMDAKARQDCVKEIGLLKQLNHPNIIKYLDSFIEDNELNIVLELADAGDLSQMIKYFKKQKRLIPERTVWKYFVQLCSAVEHMHSRRVMHRDIKPANVFITATGIVKLGDLGLGRFFSSETTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLFSLCQKIEQCDYPPLPGEHYSEKLRELVSMCIYPDPDHRPDIVYVHQVARQMHVWTSST
InterPro domains: - IPR008271: Serine/threonine-protein kinase, active site (active_site) [168-180]
- IPR001245: Serine-threonine/tyrosine-protein kinase, catalytic domain (domain) [122-296]
- IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [25-307]
- IPR017441: Protein kinase, ATP binding site (binding_site) [51-74]
- IPR000719: Protein kinase domain (domain) [45-310]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0023056 (positive regulation of signaling), GO:0048584 (positive regulation of response to stimulus), GO:0019222 (regulation of metabolic process), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:0071840 (cellular component organization or biogenesis), GO:0051239 (regulation of multicellular organismal process), GO:0071704 (organic substance metabolic process), GO:0050793 (regulation of developmental process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0023051 (regulation of signaling), GO:0007017 (microtubule-based process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0009967 (positive regulation of signal transduction), GO:0045597 (positive regulation of cell differentiation), GO:1901564 (organonitrogen compound metabolic process), GO:0044085 (cellular component biogenesis), GO:1903047 (mitotic cell cycle process), GO:0007051 (spindle organization), GO:0019538 (protein metabolic process), GO:0009966 (regulation of signal transduction), GO:0000226 (microtubule cytoskeleton organization), GO:0043170 (macromolecule metabolic process), GO:0006793 (phosphorus metabolic process), GO:0051726 (regulation of cell cycle), GO:0007059 (chromosome segregation), GO:0010646 (regulation of cell communication), GO:0010647 (positive regulation of cell communication), GO:0016043 (cellular component organization), GO:0031323 (regulation of cellular metabolic process), GO:0051128 (regulation of cellular component organization), GO:0010564 (regulation of cell cycle process), GO:0000278 (mitotic cell cycle), GO:1902850 (microtubule cytoskeleton organization involved in mitosis), GO:0007346 (regulation of mitotic cell cycle), GO:2000772 (regulation of cellular senescence), GO:0010256 (endomembrane system organization), GO:0006796 (phosphate-containing compound metabolic process), GO:0007052 (mitotic spindle organization), GO:0022607 (cellular component assembly), GO:0036211 (protein modification process), GO:0051225 (spindle assembly), GO:0051983 (regulation of chromosome segregation), GO:0043412 (macromolecule modification), GO:1901987 (regulation of cell cycle phase transition), GO:0033043 (regulation of organelle organization), GO:1902533 (positive regulation of intracellular signal transduction), GO:0006996 (organelle organization), GO:0022411 (cellular component disassembly), GO:1902531 (regulation of intracellular signal transduction), GO:0061024 (membrane organization), GO:0070925 (organelle assembly), GO:0030397 (membrane disassembly), GO:0033045 (regulation of sister chromatid segregation), GO:0006998 (nuclear envelope organization), GO:0006468 (protein phosphorylation), GO:0018193 (peptidyl-amino acid modification), GO:0016310 (phosphorylation), GO:0071763 (nuclear membrane organization), GO:1902099 (regulation of metaphase/anaphase transition of cell cycle), GO:0007010 (cytoskeleton organization), GO:0006997 (nucleus organization), GO:1901990 (regulation of mitotic cell cycle phase transition), GO:0043122 (regulation of I-kappaB kinase/NF-kappaB signaling), GO:0043123 (positive regulation of I-kappaB kinase/NF-kappaB signaling), GO:0033044 (regulation of chromosome organization), GO:0030071 (regulation of mitotic metaphase/anaphase transition), GO:0051081 (nuclear membrane disassembly), GO:0140694 (non-membrane-bounded organelle assembly), GO:0018105 (peptidyl-serine phosphorylation), GO:0018209 (peptidyl-serine modification), GO:0046777 (protein autophosphorylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)