Protein: Genome polyprotein [Cleaved into: Leader protein (L); Capsid protein VP0; Capsid protein VP3 (P1C) (Virion protein 3); Capsid protein VP1 (P1D) (Virion protein 1); Protein 2A (P2A); Protein 2B (P2B); Protein 2C (P2C) (EC 3.6.4.13); Protein 3A (P3A); VPg (P3B) (Protein 3B); Protein 3CD (EC 3.4.22.28); Protease 3C (EC 3.4.22.28) (Picornain 3C) (P3C); RNA-directed RNA polymerase (RdRp) (EC 2.7.7.48) (3D polymerase) (3Dpol) (Protein 3D) (3D)] (O91464)
Organism: Aichi virus (strain Human/A846/88/1989) (AiV) (Aichi virus (strain A846/88))
Function: Required for viral RNA replication and viral RNA encapsidation. Does not have any proteolytic activity.
Subcellular location: [Capsid protein VP0]: Virion {ECO:0000269|PubMed:27595320, ECO:0000269|PubMed:27681122}. Host cytoplasm {ECO:0000250|UniProtKB:P12296}.; SUBCELLULAR LOCATION: [Capsid protein VP3]: Virion {ECO:0000269|PubMed:27595320, ECO:0000269|PubMed:27681122}. Host cytoplasm {ECO:0000250|UniProtKB:P12296}.; SUBCELLULAR LOCATION: [Capsid protein VP1]: Virion {ECO:0000269|PubMed:27595320, ECO:0000269|PubMed:27681122}. Host cytoplasm {ECO:0000250|UniProtKB:P12296}.; SUBCELLULAR LOCATION: [Protein 2B]: Host cytoplasmic vesicle membrane {ECO:0000269|PubMed:22124328, ECO:0000269|PubMed:24672044}; Peripheral membrane protein {ECO:0000250|UniProtKB:P03304}; Cytoplasmic side {ECO:0000250|UniProtKB:P03304}. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are probably autophagosome-like vesicles. {ECO:0000250|UniProtKB:P03304}.; SUBCELLULAR LOCATION: [Protein 2C]: Host cytoplasmic vesicle membrane {ECO:0000269|PubMed:22124328, ECO:0000269|PubMed:24672044}; Peripheral membrane protein {ECO:0000250|UniProtKB:P03304}; Cytoplasmic side {ECO:0000250|UniProtKB:P03304}. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are probably autophagosome-like vesicles. {ECO:0000250|UniProtKB:P03304}.; SUBCELLULAR LOCATION: [Protein 3A]: Host cytoplasmic vesicle membrane {ECO:0000269|PubMed:22124328, ECO:0000269|PubMed:24672044}; Single-pass membrane protein {ECO:0000255}. Host Golgi apparatus membrane {ECO:0000269|PubMed:30755512}; Single-pass membrane protein {ECO:0000255}. Note=Probably localizes to intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. {ECO:0000250|UniProtKB:P03304}.; SUBCELLULAR LOCATION: [VPg]: Virion {ECO:0000250|UniProtKB:P03304}.; SUBCELLULAR LOCATION: [Protease 3C]: Host cytoplasm {ECO:0000250|UniProtKB:P03304}.; SUBCELLULAR LOCATION: [RNA-directed RNA polymerase]: Host cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:P03304}; Peripheral membrane protein {ECO:0000250|UniProtKB:P03304}; Cytoplasmic side {ECO:0000250|UniProtKB:P03304}. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are probably autophagosome-like vesicles. {ECO:0000250|UniProtKB:P03304}.
Sequence: MAATRVSRSVLAAVAHSAAHRTYHTVLDCYDRLYLNTNPHLSYPLPKNSSFPCPFCQYDEQNEVLSPESLRGEGAEPCWKCSQDKPRRKYNTTPPEDWLYDSDVQSWFYPETYYSDLQQKFFDKLALLSLPGAYQAKTPEERALAGALTQLLNFPSTPPLTLPTTNLQRQGNSVTNIYGNGNNVTTDVGANGWAPTVSTGLGDGPVSASADSLPGRSGGASSEKTHTVSGSSNKVGSRFSKWWEPAAARASESATDSAIEGIDAAGKAASKAITRKLDRPAAPSSTANPQPSLIALNPSATQSGNASILTGSTAPSLLAYPTATPVPLPNPDEPSQPGPSGDRTWLLDTVTWSQEFTRGWNIAGSNGMQWTGLESLIFPVSTDTNWTSTSSPTAYPLPFSFVRAYPDSSWAAMYNTHSMWNCGWRVQVTVNGSQFHAGALILYMVPEATTHAIQTARDNAGFVFPYVILNLYESNTATIEVPYISPTPNTSSGLHAPWTFYLQVLSPLNPPPSLPTSLSCSIYVTPVDSSFHGLRYLAPQHWKTRAVPGAGTFGSAVAGQELPLCGVRAYYPPNAYIPAQVRDWLEFAHRPGLMATVPWTMADEPAERLGIFPVSPSAIAGTGAPISYVISLFSQWRGELAAHLLFTGSAQHYGRLVVCYTPAAPQPPSTMQEAMRGTYTVWDVNAASTLEFTIPFISNSYWKTVDVNNPDALLSTTGYVSIWVQNPLVGPHTAPASALVQAFISAGESFNVRLMQNPALTSQTLTEDLDAPQDTGNIENGAADNSPQPRTTFDYTGNPLPPDTKLENFFSFYRLLPMGGSGAPSLSFPADEGTIIPLNPINWLKGADVSGIAAMLSCFTYIAADLRITLRFSNPNDNPATMLVAFAPPGATIPLKPTRQMLSNFYMAEVPVSAATSTMVSFSIPYTSPLSAIPTSYFGWEDWSGTNFGQLSSGSWGNLMLIPSLSVDSAIPFDFQLSCWVAFGNFKAWVPRPPPPLPPLPTPAANAERTVAVIKQGAASATPDVDPDDRVYIVRAQRPTYVHWAIRKVAPDGSAKQISLSRSGIQALVALEPPEGEPYLEILPSHWTLAELQLGNKWEYSATNNCTHFVSSITGESLPNTGFSLALGIGALTAIAASAAVAVKALPGIRRQGLLTLSADTETNQTLNKITESVNQAAQVVSQFDLSGPANSVSLAASDIREAAHKVASSLNGFTDVIADIKDSLFTRVSDAVESGVATFLTWLVKLFGYLLVLFGSPTPMSISGLLVIICADLAPHAREFFTASGNVLSSLYYWIASKLGLSVTPQECERATLEPQGLKDFNDGALAMRNVEWIGETAWKWAHRLLDWIRGKAKTDPQAKLADVHDEIMLHYSDSILALGSEKLPIDHITKSISRCRELVSIAQEAKSGPHSSFLNQAIKNYTLAISQHRKCQTGPRPEPVVVYLYGPPGTGKSLLASLLAQTLSQRLAGTPDDVYSPSSASCEYFDGYTGQTVHFIDDIGQDPEGRDWANFPNLVSSAPFIVPMASLEEKGTHYTSKVIVVTSNFHEPNERAARSMGALRRRVHLRINVTSNGVPFDPTNALNPIPGTQSKYFTAQTPLTLFQSNTVRLDRDSIWTPTFTNMDELVDAIVTRLDRSTGVSNSLASLIRRQGNRVIDAEPREIPLEYADDLLEAMAHHRPVPCSLGLSQAIANNTPIQQISETFWKYRKPIFTCTTFLAVLGFLCSVIPLARSLWKSKQDTPQEPQAAYSAISHQKPKPKSQKPVPTRHIQRQGISPAVPGISNNVVHVESGNGLNKNVMSGFYIFSRFLLVPTHLREPHHTTLTVGADTYDWATLQTQEFGEITIVHTPTSRQYKDMRRFIGAHPHPTGLLVSQFKAAPLYVRISDNRILDLDFPGVVVCKQAYGYRAATFEGLCGSPLVTDDPSGVKILGLHVAGVAGTSGFSAPIHPILGQITQFATTQQSLIVPTAEVRPGVNVNRMSRLHPSPAYGAFPVKKQPAPLKRNDKRLQEGVDLDTQLFLKHGKGDVTEPWPGLEAAADLYFSTFPTSLPVLTQEQAIHGTPNMEGLDMGQAAGYPWNTLGRSRRSLFDEVEPGVFVPKPELQAEINQTLEDPDYVYSTFLKDELRPTAKVEQGLTRIVEAAPIHAIVAGRMLLGGLIDYMQGRPGEHGSAVGCNPDVHWTSFFYAFSEFSQVYDLDYKCFDATLPSAVFTLVADHLTRITGDPRVGRYIHSIRHSHHIYGNRMYDMIGGNPSGCVATSILNTIINNICVLSALIQHPDFSPSRFHILAYGDDVIYATEPPIHPSFLREFYQKHTPLVVTPANKGQDFPPTSTIYEVTFLKRWFVPDDVRPIYIHPVMDPDTYEQSVMWLRDGDFQDVVTSLCHLAFHSGPKTYAAWCMKVREQCLKSGFAPNFLPYSYLQLRWLNLLAA
InterPro domains: - IPR004004: Helicase/polymerase/peptidase polyprotein, Calicivirus-type (family) [1438-2302]
- IPR043504: Peptidase S1, PA clan, chymotrypsin-like fold (homologous_superfamily) [1800-1948]
- IPR001205: RNA-directed RNA polymerase, C-terminal domain (domain) [1991-2399]
- IPR043502: DNA/RNA polymerase superfamily (homologous_superfamily) [1967-2429]
- IPR007053: LRAT domain (domain) [1033-1121]
- IPR014759: Helicase, superfamily 3, single-stranded RNA virus (domain) [1422-1586]
- IPR043128: Reverse transcriptase/Diguanylate cyclase domain (homologous_superfamily) [2168-2343]
- IPR000199: Peptidase C3A/C3B, picornaviral (domain) [1783-1938]
- IPR001676: Picornavirus capsid (domain) [378-723]
- IPR000605: Helicase, superfamily 3, single-stranded DNA/RNA virus (domain) [1444-1547]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [1319-1567]
- IPR029053: Viral coat protein subunit (homologous_superfamily) [298-1018]
- IPR033703: Picornavirus/Calicivirus coat protein (domain) [378-990]
- IPR009003: Peptidase S1, PA clan (homologous_superfamily) [1785-1957]
- IPR007094: RNA-directed RNA polymerase, catalytic domain (domain) [2193-2308]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0140098 (catalytic activity, acting on RNA), GO:0008233 (peptidase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0008234 (cysteine-type peptidase activity), GO:0004175 (endopeptidase activity), GO:0016779 (nucleotidyltransferase activity), GO:0097747 (RNA polymerase activity), GO:0034062 (5'-3' RNA polymerase activity), GO:0004197 (cysteine-type endopeptidase activity), GO:0003968 (RNA-dependent RNA polymerase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0016032 (viral process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0044068 (modulation by symbiont of host cellular process), GO:0019048 (modulation by virus of host process), GO:0044403 (biological process involved in symbiotic interaction), GO:0035821 (modulation of process of another organism), GO:0051701 (biological process involved in interaction with host), GO:0019054 (modulation by virus of host cellular process), GO:0044003 (modulation by symbiont of host process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0044423 (virion component), GO:0019028 (viral capsid), GO:0019030 (icosahedral viral capsid)