Protein: Rho-related GTP-binding protein RhoH (Q9D3G9)
Organism: Mus musculus (Mouse)
Function: Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils (By similarity). Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:17028588}. Cell membrane {ECO:0000269|PubMed:17028588}; Lipid-anchor {ECO:0000269|PubMed:17028588}; Cytoplasmic side {ECO:0000269|PubMed:17028588}. Note=Colocalizes together with ZAP70 in the immunological synapse.
Sequence: MLSSIKCVLVGDSAVGKTSLLVRFTSETFPEAYKPTVYENTGVDVFMDGIQISLGLWDTAGNDAFRSIRPLSYQQADVVLMCYSVANHNSFLNLKNKWISEIRSNLPCTPVLVVATQTDQREVGPHRASCINAIEGKRLAQDVRAKGYLECSALSNRGVQQVFECAVRTAVNQARRRNRRKLFSINECKIF
InterPro domains: - IPR001806: Small GTPase (family) [1-191]
- IPR003578: Small GTPase Rho (family) [4-175]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [2-184]
- IPR005225: Small GTP-binding domain (domain) [4-139]
Interaction partners: - Rho-related GTP-binding protein RhoB
- Tyrosine-protein kinase ZAP-70
- Intersectin-2
- Intersectin-1
- Proto-oncogene tyrosine-protein kinase LCK
- Rho GDP-dissociation inhibitor 1
- Rho GDP-dissociation inhibitor 2
- Rho GDP-dissociation inhibitor 3
- Rho-associated protein kinase 1
- Rho-associated protein kinase 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0005488 (binding), GO:0030234 (enzyme regulator activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:0005515 (protein binding), GO:0140678 (molecular function inhibitor activity), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0032553 (ribonucleotide binding), GO:0019207 (kinase regulator activity), GO:0004857 (enzyme inhibitor activity), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0019210 (kinase inhibitor activity), GO:0032555 (purine ribonucleotide binding), GO:0017076 (purine nucleotide binding), GO:0005525 (GTP binding), GO:0019001 (guanyl nucleotide binding), GO:0032561 (guanyl ribonucleotide binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0050793 (regulation of developmental process), GO:0023057 (negative regulation of signaling), GO:0051240 (positive regulation of multicellular organismal process), GO:0002684 (positive regulation of immune system process), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0002682 (regulation of immune system process), GO:0023051 (regulation of signaling), GO:0051239 (regulation of multicellular organismal process), GO:0051094 (positive regulation of developmental process), GO:0048523 (negative regulation of cellular process), GO:0048585 (negative regulation of response to stimulus), GO:0048522 (positive regulation of cellular process), GO:0045597 (positive regulation of cell differentiation), GO:0009968 (negative regulation of signal transduction), GO:0002696 (positive regulation of leukocyte activation), GO:0010648 (negative regulation of cell communication), GO:2000026 (regulation of multicellular organismal development), GO:0010646 (regulation of cell communication), GO:0009966 (regulation of signal transduction), GO:0030155 (regulation of cell adhesion), GO:0045595 (regulation of cell differentiation), GO:0002694 (regulation of leukocyte activation), GO:1903708 (positive regulation of hemopoiesis), GO:0045785 (positive regulation of cell adhesion), GO:1903706 (regulation of hemopoiesis), GO:0050865 (regulation of cell activation), GO:0050867 (positive regulation of cell activation), GO:0051251 (positive regulation of lymphocyte activation), GO:0022409 (positive regulation of cell-cell adhesion), GO:1902107 (positive regulation of leukocyte differentiation), GO:0022407 (regulation of cell-cell adhesion), GO:0060284 (regulation of cell development), GO:0010720 (positive regulation of cell development), GO:1902105 (regulation of leukocyte differentiation), GO:1902532 (negative regulation of intracellular signal transduction), GO:1902531 (regulation of intracellular signal transduction), GO:0051249 (regulation of lymphocyte activation), GO:0050870 (positive regulation of T cell activation), GO:0045619 (regulation of lymphocyte differentiation), GO:0050863 (regulation of T cell activation), GO:0043122 (regulation of I-kappaB kinase/NF-kappaB signaling), GO:0043124 (negative regulation of I-kappaB kinase/NF-kappaB signaling), GO:1903037 (regulation of leukocyte cell-cell adhesion), GO:1903039 (positive regulation of leukocyte cell-cell adhesion), GO:0045621 (positive regulation of lymphocyte differentiation), GO:0045582 (positive regulation of T cell differentiation), GO:0045580 (regulation of T cell differentiation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0009986 (cell surface)