Protein: Probable guanine deaminase (Guanase) (Guanine aminase) (EC 3.5.4.3) (Guanine aminohydrolase) (GAH) (Q07729)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:14562095}.
Sequence: MTKSDLLFDKFNDKHGKFLVFFGTFVDTPKLGELRIREKTSVGVLNGIIRFVNRNSLDPVKDCLDHDSSLSPEDVTVVDIIGKDKTRNNSFYFPGFVDTHNHVSQYPNVGVFGNSTLLDWLEKYTFPIEAALANENIAREVYNKVISKTLSHGTTTVAYYNTIDLKSTKLLAQLSSLLGQRVLVGKVCMDTNGPEYYIEDTKTSFESTVKVVKYIRETICDPLVNPIVTPRFAPSCSRELMQQLSKLVKDENIHVQTHLSENKEEIQWVQDLFPECESYTDVYDKYGLLTEKTVLAHCIHLTDAEARVIKQRRCGISHCPISNSSLTSGECRVRWLLDQGIKVGLGTDVSAGHSCSILTTGRQAFAVSRHLAMRETDHAKLSVSECLFLATMGGAQVLRMDETLGTFDVGKQFDAQMIDTNAPGSNVDMFHWQLKEKDQMQEQEQEQGQDPYKNPPLLTNEDIIAKWFFNGDDRNTTKVWVAGQQVYQI
InterPro domains: - IPR051607: Metallo-dependent Hydrolases (family) [16-487]
- IPR011059: Metal-dependent hydrolase, composite domain superfamily (homologous_superfamily) [21-485]
- IPR014311: Guanine deaminase (family) [21-483]
- IPR006680: Amidohydrolase-related (domain) [92-486]
- IPR032466: Metal-dependent hydrolase (homologous_superfamily) [94-405]
Interaction partners: - Purine nucleoside phosphorylase
- Adenine phosphoribosyltransferase 2
- Adenine phosphoribosyltransferase 1
- Hypoxanthine-guanine phosphoribosyltransferase
- Uridine nucleosidase
- Adenine deaminase
- Adenylosuccinate lyase
- Cytidine deaminase
- Phosphate metabolism protein 8
- S-methyl-5'-thioadenosine phosphorylase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0016810 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds), GO:0019239 (deaminase activity), GO:0016814 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0044248 (cellular catabolic process), GO:0044249 (cellular biosynthetic process), GO:0043094 (cellular metabolic compound salvage), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0009112 (nucleobase metabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0043101 (purine-containing compound salvage), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0019439 (aromatic compound catabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0046700 (heterocycle catabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0072521 (purine-containing compound metabolic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0006144 (purine nucleobase metabolic process), GO:0072523 (purine-containing compound catabolic process), GO:0046113 (nucleobase catabolic process), GO:0046112 (nucleobase biosynthetic process), GO:0072522 (purine-containing compound biosynthetic process), GO:0006145 (purine nucleobase catabolic process), GO:0009113 (purine nucleobase biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)