Protein: 3 beta-hydroxysteroid dehydrogenase type 7 (3 beta-hydroxysteroid dehydrogenase type VII) (3-beta-HSD VII) (3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase) (C(27) 3-beta-HSD) (EC 1.1.1.-) (Cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase) (EC 1.1.1.181) (Q9H2F3)
Organism: Homo sapiens (Human)
Function: The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis. Plays a key role in cell positioning and movement in lymphoid tissues by mediating degradation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC): 7-alpha,25-OHC acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells.
Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence: MADSAQAQKLVYLVTGGCGFLGEHVVRMLLQREPRLGELRVFDQHLGPWLEELKTGPVRVTAIQGDVTQAHEVAAAVAGAHVVIHTAGLVDVFGRASPKTIHEVNVQGTRNVIEACVQTGTRFLVYTSSMEVVGPNTKGHPFYRGNEDTPYEAVHRHPYPCSKALAEWLVLEANGRKVRGGLPLVTCALRPTGIYGEGHQIMRDFYRQGLRLGGWLFRAIPASVEHGRVYVGNVAWMHVLAARELEQRATLMGGQVYFCYDGSPYRSYEDFNMEFLGPCGLRLVGARPLLPYWLLVFLAALNALLQWLLRPLVLYAPLLNPYTLAVANTTFTVSTDKAQRHFGYEPLFSWEDSRTRTILWVQAATGSAQ
InterPro domains: - IPR002225: 3-beta hydroxysteroid dehydrogenase/isomerase (domain) [13-283]
- IPR036291: NAD(P)-binding domain superfamily (homologous_superfamily) [11-362]
- IPR050425: NAD(P)-dependent dehydratase-like (family) [11-259]
Interaction partners: - Aldo-keto reductase family 1 member D1
- Cytochrome P450 7B1
- 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
- Cytochrome P450 7A1
- 24-hydroxycholesterol 7-alpha-hydroxylase
- Ubiquitin-like protein FUBI
- Cholesterol 25-hydroxylase
- Alpha-methylacyl-CoA racemase
- 3-keto-steroid reductase/17-beta-hydroxysteroid dehydrogenase 7
- Lanosterol 14-alpha demethylase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016853 (isomerase activity), GO:0005515 (protein binding), GO:0016491 (oxidoreductase activity), GO:0016860 (intramolecular oxidoreductase activity), GO:0016614 (oxidoreductase activity, acting on CH-OH group of donors), GO:0016229 (steroid dehydrogenase activity), GO:0016616 (oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor), GO:0033764 (steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor), GO:0016863 (intramolecular oxidoreductase activity, transposing C=C bonds)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0065008 (regulation of biological quality), GO:0009058 (biosynthetic process), GO:0042445 (hormone metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0008210 (estrogen metabolic process), GO:0010817 (regulation of hormone levels), GO:0008209 (androgen metabolic process), GO:0042446 (hormone biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0008202 (steroid metabolic process), GO:0006703 (estrogen biosynthetic process), GO:0006702 (androgen biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0006694 (steroid biosynthetic process), GO:0120178 (steroid hormone biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)