Protein: VPS35 endosomal protein-sorting factor-like (Esophageal cancer-associated protein) (Q7Z3J2)
Organism: Homo sapiens (Human)
Function: Acts as component of the retriever complex. The retriever complex is a heterotrimeric complex related to retromer cargo-selective complex (CSC) and essential for retromer-independent retrieval and recycling of numerous cargos such as integrin alpha-5/beta-1 (ITGA5:ITGB1). The recruitment of the retriever complex to the endosomal membrane involves CCC and WASH complexes. In the endosomes, drives the retrieval and recycling of NxxY-motif-containing cargo proteins by coupling to SNX17, a cargo essential for the homeostatic maintenance of numerous cell surface proteins associated with processes that include cell migration, cell adhesion, nutrient supply and cell signaling. Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association with the CCC complex and cooperation with the WASH complex on early endosomes. Seems not to be required for CCC complex stability.
Subcellular location: Membrane {ECO:0000255}; Single-pass membrane protein {ECO:0000255}. Endosome {ECO:0000269|PubMed:25355947, ECO:0000269|PubMed:28892079}. Note=Endosome location is dependent of the association with the CCC and WASH complexes. {ECO:0000269|PubMed:28892079}.
Sequence: MAVFPWHSRNRNYKAEFASCRLEAVPLEFGDYHPLKPITVTESKTKKVNRKGSTSSTSSSSSSSVVDPLSSVLDGTDPLSMFAATADPAALAAAMDSSRRKRDRDDNSVVGSDFEPWTNKRGEILARYTTTEKLSINLFMGSEKGKAGTATLAMSEKVRTRLEELDDFEEGSQKELLNLTQQDYVNRIEELNQSLKDAWASDQKVKALKIVIQCSKLLSDTSVIQFYPSKFVLITDILDTFGKLVYERIFSMCVDSRSVLPDHFSPENANDTAKETCLNWFFKIASIRELIPRFYVEASILKCNKFLSKTGISECLPRLTCMIRGIGDPLVSVYARAYLCRVGMEVAPHLKETLNKNFFDFLLTFKQIHGDTVQNQLVVQGVELPSYLPLYPPAMDWIFQCISYHAPEALLTEMMERCKKLGNNALLLNSVMSAFRAEFIATRSMDFIGMIKECDESGFPKHLLFRSLGLNLALADPPESDRLQILNEAWKVITKLKNPQDYINCAEVWVEYTCKHFTKREVNTVLADVIKHMTPDRAFEDSYPQLQLIIKKVIAHFHDFSVLFSVEKFLPFLDMFQKESVRVEVCKCIMDAFIKHQQEPTKDPVILNALLHVCKTMHDSVNALTLEDEKRMLSYLINGFIKMVSFGRDFEQQLSFYVESRSMFCNLEPVLVQLIHSVNRLAMETRKVMKGNHSRKTAAFVRACVAYCFITIPSLAGIFTRLNLYLHSGQVALANQCLSQADAFFKAAISLVPEVPKMINIDGKMRPSESFLLEFLCNFFSTLLIVPDHPEHGVLFLVRELLNVIQDYTWEDNSDEKIRIYTCVLHLLSAMSQETYLYHIDKVDSNDSLYGGDSKFLAENNKLCETVMAQILEHLKTLAKDEALKRQSSLGLSFFNSILAHGDLRNNKLNQLSVNLWHLAQRHGCADTRTMVKTLEYIKKQSKQPDMTHLTELALRLPLQTRT
InterPro domains: - IPR029705: VPS35 endosomal protein sorting factor-like (family) [6-962]
Interaction partners: - Vacuolar protein sorting-associated protein 29
- Vacuolar protein sorting-associated protein 26C
- Coiled-coil domain-containing protein 22
- Coiled-coil domain-containing protein 93
- COMM domain-containing protein 1
- COMM domain-containing protein 6
- DENN domain-containing protein 10
- COMM domain-containing protein 2
- COMM domain-containing protein 3
- COMM domain-containing protein 4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0051641 (cellular localization), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0051234 (establishment of localization), GO:0051716 (cellular response to stimulus), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0044238 (primary metabolic process), GO:0033036 (macromolecule localization), GO:1901360 (organic cyclic compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0046907 (intracellular transport), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006810 (transport), GO:0070727 (cellular macromolecule localization), GO:0051649 (establishment of localization in cell), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0051668 (localization within membrane), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0045184 (establishment of protein localization), GO:0006886 (intracellular protein transport), GO:0071705 (nitrogen compound transport), GO:0090304 (nucleic acid metabolic process), GO:0015031 (protein transport), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0071702 (organic substance transport), GO:0008104 (protein localization), GO:0006996 (organelle organization), GO:0010467 (gene expression), GO:0090150 (establishment of protein localization to membrane), GO:0072657 (protein localization to membrane), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0042254 (ribosome biogenesis), GO:0033365 (protein localization to organelle), GO:0016070 (RNA metabolic process), GO:0022618 (ribonucleoprotein complex assembly), GO:0006605 (protein targeting), GO:0007033 (vacuole organization), GO:0065003 (protein-containing complex assembly), GO:0090305 (nucleic acid phosphodiester bond hydrolysis), GO:0002181 (cytoplasmic translation), GO:0006396 (RNA processing), GO:0071826 (ribonucleoprotein complex subunit organization), GO:0034504 (protein localization to nucleus), GO:0034470 (ncRNA processing), GO:0006364 (rRNA processing), GO:0034660 (ncRNA metabolic process), GO:0140694 (non-membrane-bounded organelle assembly), GO:0016072 (rRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0031974 (membrane-enclosed lumen), GO:0005829 (cytosol), GO:0098796 (membrane protein complex), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0030141 (secretory granule), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043228 (non-membrane-bounded organelle), GO:0005773 (vacuole), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0060205 (cytoplasmic vesicle lumen), GO:0000323 (lytic vacuole), GO:0031983 (vesicle lumen), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0034774 (secretory granule lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0012506 (vesicle membrane), GO:0005770 (late endosome), GO:0005769 (early endosome), GO:0030667 (secretory granule membrane), GO:0005774 (vacuolar membrane), GO:0010008 (endosome membrane), GO:0031982 (vesicle), GO:0070013 (intracellular organelle lumen), GO:0030659 (cytoplasmic vesicle membrane), GO:0005764 (lysosome), GO:0097708 (intracellular vesicle), GO:0031901 (early endosome membrane), GO:0031902 (late endosome membrane), GO:0005694 (chromosome), GO:0098852 (lytic vacuole membrane), GO:0005765 (lysosomal membrane)