Protein: Heat shock factor protein (HSF) (Heat shock transcription factor) (HSTF) (P22813)
Organism: Drosophila melanogaster (Fruit fly)
Function: DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Subcellular location: Nucleus.
Sequence: MSRSRSSAKAVQFKHESEEEEEDEEEQLPSRRMHSYGDAAAIGSGVPAFLAKLWRLVDDADTNRLICWTKDGQSFVIQNQAQFAKELLPLNYKHNNMASFIRQLNMYGFHKITSIDNGGLRFDRDEIEFSHPFFKRNSPFLLDQIKRKISNNKNGDDKGVLKPEAMSKILTDVKVMRGRQDNLDSRFSAMKQENEVLWREIASLRQKHAKQQQIVNKLIQFLITIVQPSRNMSGVKRHVQLMINNTPEIDRARTTSETESESGGGPVIHELREELLDEVMNPSPAGYTAASHYDQESVSPPAVERPRSNMSISSHNVDYSNQSVEDLLLQGNGTAGGNILVGGAASPMAQSVSQSPAQHDVYTVTEAPDSHVQEVPNSPPYYEEQNVLTTPMVREQEQQKRQQLKENNKLRRQAGDVILDAGDILVDSSSPKAQRTSIQHSTQPDVMVQPMIIKSEPENSSGLMDLMTPANDLYSVNFISEDMPTDIFEDALLPDGVEEAAKLDQQQKFGQSTVSSGKFASNFDVPTNSTLLDANQASTSKAAAKAQASEEEGMAVAKYSGAENGNNRDTNNSQLLRMASVDELHGHLESMQDELETLKDLLRGDGVAIDQNMLMGLFNDSDLMDNYGLSFPNDSISSEKKAPSGSELISYQPMYDLSDILDTDDGNNDQEASRRQMQTQSSVLNTPRHEL
InterPro domains: - IPR000232: Heat shock factor (HSF)-type, DNA-binding (domain) [45-148]
- IPR036390: Winged helix DNA-binding domain superfamily (homologous_superfamily) [44-148]
- IPR010542: Vertebrate heat shock transcription factor, C-terminal domain (domain) [530-633]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [43-148]
Interaction partners: - Heat shock protein 83
- Major heat shock 70 kDa protein Ba
- Heat shock protein 83
- Cytosolic Prostaglandin E synthase
- Elongation factor 1-alpha 1
- Elongation factor 1-alpha 2
- Heat shock factor-binding protein 1
- Transitional endoplasmic reticulum ATPase TER94
- 14-3-3 protein epsilon
- Protein deacetylase HDAC6
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:1901363 (heterocyclic compound binding), GO:0003700 (DNA-binding transcription factor activity), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0001216 (DNA-binding transcription activator activity), GO:0046983 (protein dimerization activity), GO:0042802 (identical protein binding), GO:0000981 (DNA-binding transcription factor activity, RNA polymerase II-specific), GO:0001228 (DNA-binding transcription activator activity, RNA polymerase II-specific), GO:0001067 (transcription regulatory region nucleic acid binding), GO:0042803 (protein homodimerization activity), GO:0003677 (DNA binding), GO:0003690 (double-stranded DNA binding), GO:0043565 (sequence-specific DNA binding), GO:0000976 (transcription cis-regulatory region binding), GO:1990837 (sequence-specific double-stranded DNA binding), GO:0000987 (cis-regulatory region sequence-specific DNA binding), GO:0000977 (RNA polymerase II transcription regulatory region sequence-specific DNA binding), GO:0000978 (RNA polymerase II cis-regulatory region sequence-specific DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0022414 (reproductive process), GO:0002376 (immune system process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0019953 (sexual reproduction), GO:0048856 (anatomical structure development), GO:0023057 (negative regulation of signaling), GO:0042221 (response to chemical), GO:0006955 (immune response), GO:0002684 (positive regulation of immune system process), GO:0032504 (multicellular organism reproduction), GO:0003006 (developmental process involved in reproduction), GO:0009892 (negative regulation of metabolic process), GO:0022412 (cellular process involved in reproduction in multicellular organism), GO:0009607 (response to biotic stimulus), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0002253 (activation of immune response), GO:0048523 (negative regulation of cellular process), GO:0051707 (response to other organism), GO:0007165 (signal transduction), GO:0048869 (cellular developmental process), GO:0009628 (response to abiotic stimulus), GO:0007275 (multicellular organism development), GO:0048584 (positive regulation of response to stimulus), GO:0019222 (regulation of metabolic process), GO:0002682 (regulation of immune system process), GO:0009893 (positive regulation of metabolic process), GO:0006950 (response to stress), GO:0048609 (multicellular organismal reproductive process), GO:0048585 (negative regulation of response to stimulus), GO:0007154 (cell communication), GO:0048522 (positive regulation of cellular process), GO:0009266 (response to temperature stimulus), GO:0009968 (negative regulation of signal transduction), GO:0032103 (positive regulation of response to external stimulus), GO:0043207 (response to external biotic stimulus), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0080134 (regulation of response to stress), GO:0048731 (system development), GO:0009966 (regulation of signal transduction), GO:0060255 (regulation of macromolecule metabolic process), GO:0032101 (regulation of response to external stimulus), GO:0002700 (regulation of production of molecular mediator of immune response), GO:0002218 (activation of innate immune response), GO:0009266 (response to temperature stimulus), GO:0009894 (regulation of catabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0050778 (positive regulation of immune response), GO:0031349 (positive regulation of defense response), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0002764 (immune response-regulating signaling pathway), GO:0007276 (gamete generation), GO:0007281 (germ cell development), GO:0010648 (negative regulation of cell communication), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0006979 (response to oxidative stress), GO:0048515 (spermatid differentiation), GO:0006952 (defense response), GO:0030522 (intracellular receptor signaling pathway), GO:0031324 (negative regulation of cellular metabolic process), GO:0010033 (response to organic substance), GO:0009891 (positive regulation of biosynthetic process), GO:0007283 (spermatogenesis), GO:0098542 (defense response to other organism), GO:0002831 (regulation of response to biotic stimulus), GO:0050776 (regulation of immune response), GO:0002757 (immune response-activating signaling pathway), GO:0031325 (positive regulation of cellular metabolic process), GO:0010646 (regulation of cell communication), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0002833 (positive regulation of response to biotic stimulus), GO:0009889 (regulation of biosynthetic process), GO:0070727 (cellular macromolecule localization), GO:0031323 (regulation of cellular metabolic process), GO:0010647 (positive regulation of cell communication), GO:0080090 (regulation of primary metabolic process), GO:0009896 (positive regulation of catabolic process), GO:0031347 (regulation of defense response), GO:0045089 (positive regulation of innate immune response), GO:0002758 (innate immune response-activating signaling pathway), GO:0010556 (regulation of macromolecule biosynthetic process), GO:1900426 (positive regulation of defense response to bacterium), GO:0048880 (sensory system development), GO:0002760 (positive regulation of antimicrobial humoral response), GO:0007286 (spermatid development), GO:0010468 (regulation of gene expression), GO:0048666 (neuron development), GO:0007399 (nervous system development), GO:0010508 (positive regulation of autophagy), GO:0007292 (female gamete generation), GO:0010508 (positive regulation of autophagy), GO:0050688 (regulation of defense response to virus), GO:0034605 (cellular response to heat), GO:0051252 (regulation of RNA metabolic process), GO:0002920 (regulation of humoral immune response), GO:1901701 (cellular response to oxygen-containing compound), GO:0010629 (negative regulation of gene expression), GO:0070925 (organelle assembly), GO:0002221 (pattern recognition receptor signaling pathway), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0150063 (visual system development), GO:0050829 (defense response to Gram-negative bacterium), GO:1902680 (positive regulation of RNA biosynthetic process), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0080171 (lytic vacuole organization), GO:0045944 (positive regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000785 (chromatin), GO:0005737 (cytoplasm), GO:0000791 (euchromatin), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome), GO:0005634 (nucleus)