Protein: Peroxisomal biogenesis factor 2 (EC 2.3.2.36) (Peroxin-2) (P32800)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX2 also regulates peroxisome organization by acting as a E3 ubiquitin-protein ligase. PEX2 ubiquitinates PEX5 during its passage through the retrotranslocation channel: catalyzes monoubiquitination of PEX5 at 'Cys-6', a modification that acts as a signal for PEX5 extraction into the cytosol.
Subcellular location: Peroxisome membrane {ECO:0000269|PubMed:12876220, ECO:0000269|PubMed:35768507}; Multi-pass membrane protein {ECO:0000255}.
Sequence: MSRVAQLDSIALDKELYGQFWSEFNAAFNTSEHKEEWELALNTVVFMCATRFLPHYGSSCTYGSALSGVVFQCRKRTLYVVTVLAGYVWKKITHIIFNGPHCGNQMMWLKLYKWVNLLYHGCDVTNFLRFLAAEGPNARAFLSPLYRAFNVHSTRLIRDGSAIASEFYSNSVFAGLEYQNRQLLWNALLELFSNTLLTKRGLLTFVKKPPRSRSTTTYKTVCPRCGGFPTNPYQIACCRANYCYVCVVKALEWSMCDACGSSGRLTASPVY
InterPro domains: - IPR025654: Peroxisome biogenesis factor 2/10 (family) [66-255]
- IPR006845: Pex, N-terminal (domain) [13-206]
Interaction partners: - Peroxisome assembly protein 12
- Peroxisome biogenesis factor 10
- Peroxisomal membrane protein PEX13
- Peroxisomal biogenesis factor 8
- Peroxisomal membrane protein PEX14
- Peroxisomal targeting signal receptor
- Peroxisomal membrane protein PEX17
- Peroxisomal membrane protein import receptor PEX19
- Peroxisomal membrane protein PMP27
- Ubiquitin-conjugating enzyme E2-21 kDa
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0140318 (protein transporter activity), GO:0008320 (protein transmembrane transporter activity), GO:0022884 (macromolecule transmembrane transporter activity), GO:0019787 (ubiquitin-like protein transferase activity), GO:0004842 (ubiquitin-protein transferase activity), GO:0061659 (ubiquitin-like protein ligase activity), GO:0061630 (ubiquitin protein ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0055085 (transmembrane transport), GO:0006807 (nitrogen compound metabolic process), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0033036 (macromolecule localization), GO:0044237 (cellular metabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0051649 (establishment of localization in cell), GO:0043170 (macromolecule metabolic process), GO:0045184 (establishment of protein localization), GO:0046907 (intracellular transport), GO:0071806 (protein transmembrane transport), GO:0006810 (transport), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0044260 (cellular macromolecule metabolic process), GO:0043112 (receptor metabolic process), GO:0071705 (nitrogen compound transport), GO:0072594 (establishment of protein localization to organelle), GO:0071702 (organic substance transport), GO:0065002 (intracellular protein transmembrane transport), GO:0044743 (protein transmembrane import into intracellular organelle), GO:0043574 (peroxisomal transport), GO:0036211 (protein modification process), GO:0006886 (intracellular protein transport), GO:0043412 (macromolecule modification), GO:0015031 (protein transport), GO:0043933 (protein-containing complex organization), GO:0008104 (protein localization), GO:0006996 (organelle organization), GO:0022411 (cellular component disassembly), GO:0033365 (protein localization to organelle), GO:0007031 (peroxisome organization), GO:0016558 (protein import into peroxisome matrix), GO:0070647 (protein modification by small protein conjugation or removal), GO:0001881 (receptor recycling), GO:0006625 (protein targeting to peroxisome), GO:0006605 (protein targeting), GO:0032984 (protein-containing complex disassembly), GO:0072663 (establishment of protein localization to peroxisome), GO:0015919 (peroxisomal membrane transport), GO:0072662 (protein localization to peroxisome), GO:0032446 (protein modification by small protein conjugation), GO:0016567 (protein ubiquitination), GO:0006513 (protein monoubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0140535 (intracellular protein-containing complex), GO:1990351 (transporter complex), GO:0031090 (organelle membrane), GO:0042579 (microbody), GO:1990234 (transferase complex), GO:0000151 (ubiquitin ligase complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005777 (peroxisome), GO:0098588 (bounding membrane of organelle), GO:0031903 (microbody membrane), GO:0005778 (peroxisomal membrane)