Protein: DNA endonuclease RBBP8 (EC 3.1.-.-) (CtBP-interacting protein) (CtIP) (Retinoblastoma-binding protein 8) (RBBP-8) (Retinoblastoma-interacting protein and myosin-like) (RIM) (Sporulation in the absence of SPO11 protein 2 homolog) (SAE2) (B1WC58)
Organism: Rattus norvegicus (Rat)
Function: Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage (By similarity). During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations (By similarity).
Subcellular location: Nucleus {ECO:0000250|UniProtKB:Q99708}. Chromosome {ECO:0000250|UniProtKB:Q99708}. Note=Associates with sites of DNA damage in S/G2 phase. Recruited to DSBs by the MRE11-RAD50-NBN (MRN) complex following phosphorylation by CDK1, which promotes interaction with NBN. Ubiquitinated RBBP8 binds to chromatin following DNA damage. {ECO:0000250|UniProtKB:Q99708}.
Sequence: MSISGSSCGSPNSTDISSDFKELWTKLKEYHDKEVQGLQIKVTKLKKERILDAQRLEEFFTKNQQLRDQQKVLQETIKILEDRLRAGLCDRCAVTEEHMHKKQQEFENIRQQNLRLITELMNEKSALQEENKKLSEQLQQKMESGQQDQVAELECEENIIPDSPITSFSFSGINRLRRKENLHVRYVEQTHTKLEHSACTSELRKFSKGSTPAPVNSEEHEILVADTCDQSHSPLSKICGTSSYPADKLSSNLDAVVAETLGLDGQEESEPQGPVSPLGNELYHCLKEDHKKQPFMESAIRNEDNVRFSDSASKAPPRELTTRGSSPVFGPTSTVKTHLGLKTSFSPSLLDSGKKNLLSTAPFSSISVSRSEKVRSKSEDNALFTQQSAGSEVKVISQSFPSKQILTSKNVSDSVDEQGGADHMKDAVSDKHLVPLKSLGGKASKRKRTEEEGEHAVHCPQTCFDKENALPFPMENQFPVNGDHVMDKPLDLSDRFAANQRQEKNHGDETCKHKLKQVTIYEALKPVPKGSSSGRKALSGACTLAQDSAETYCLQQRTLQCSSKFSPDHNTQLQIKEENPVFKTPPRSQESLETENLFGDVKGIGSLVPIKVKGRSAHGGCELASVLQLNPCRVAKTKSLPSNHDMSFENIQWSVDPGADLSQYKMDVTVIDTKDSSHSRLGGETVDMDCTLVSETMLLKMKKQEQREKSPNGDIKMNDSLEDMFDRTTHEEYESCLADNFSQVPDEEELSDTTKKPNIHGDKQDGIKQKAFVEPYFKDKERETSIQNFPHIEVVRKKEERRKLLGHTCKECEIYYADLPAEEREKKLASCSRHRFRYIPTNTPENFWEVGFPSTQTCLERGYIKEDLDPCPRPKRRQPYNAVFSPKGKEQRT
InterPro domains: - IPR033316: DNA endonuclease RBBP8-like (family) [10-858]
- IPR013882: DNA endonuclease activator Ctp1, C-terminal (domain) [825-856]
- IPR019518: DNA endonuclease Ctp1, N-terminal (domain) [20-139]
Interaction partners: - Breast cancer type 1 susceptibility protein homolog
- C-terminal-binding protein 1
- BRCA1-associated RING domain protein 1
- C-terminal-binding protein 2
- Double-strand break repair protein MRE11
- Nibrin
- DNA repair protein RAD50
- Exonuclease 1
- DNA replication ATP-dependent helicase/nuclease DNA2
- Breast cancer type 2 susceptibility protein homolog
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0071684 (organism emergence from protective structure), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:0001824 (blastocyst development), GO:0009790 (embryo development), GO:1903047 (mitotic cell cycle process), GO:0000278 (mitotic cell cycle), GO:0044770 (cell cycle phase transition), GO:0035188 (hatching), GO:0001835 (blastocyst hatching), GO:0044772 (mitotic cell cycle phase transition), GO:0044843 (cell cycle G1/S phase transition), GO:0009792 (embryo development ending in birth or egg hatching), GO:0043009 (chordate embryonic development), GO:0000082 (G1/S transition of mitotic cell cycle), GO:0001701 (in utero embryonic development)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0032991 (protein-containing complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140513 (nuclear protein-containing complex), GO:0005667 (transcription regulator complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0090575 (RNA polymerase II transcription regulator complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)