Protein: Calcium-transporting ATPase 3, endoplasmic reticulum-type (AtECA3) (EC 7.2.2.10) (Q9SY55)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to an endomembrane compartment (Probable). Involved in calcium-enhanced root growth, in tolerance to toxic levels of manganese and in secretory processes. Has a crucial role in manganese nutrition, but is not involved in transporting copper, iron or zinc.
Subcellular location: Golgi apparatus membrane {ECO:0000269|PubMed:18024560}; Multi-pass membrane protein {ECO:0000255}. Endosome membrane {ECO:0000269|PubMed:18567829}; Multi-pass membrane protein {ECO:0000255}. Prevacuolar compartment membrane {ECO:0000269|PubMed:18567829}; Multi-pass membrane protein {ECO:0000255}.
Sequence: MEDAYARSVSEVLDFFGVDPTKGLSDSQVVHHSRLYGRNVLPEEKRTPFWKLVLKQFDDLLVKILIVAAIVSFVLALANGETGLTAFLEPFVILLILAANAAVGVITETNAEKALEELRAYQANIATVLRNGCFSILPATELVPGDIVEVTVGCKIPADLRMIEMSSNTFRVDQAILTGESCSVEKDVDCTLTTNAVYQDKKNILFSGTDVVAGRGRAVVIGVGSNTAMGSIHDSMLQTDDEATPLKKKLDEFGSFLAKVIAGICVLVWVVNIGHFSDPSHGGFFKGAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKKMARLNAIVRSLPSVETLGCTTVICSDKTGTLTTNMMSVSKICVVQSAEHGPMINEFTVSGTTYAPEGTVFDSNGMQLDLPAQSPCLHHLAMCSSLCNDSILQYNPDKDSYEKIGESTEVALRVLAEKVGLPGFDSMPSALNMLSKHERASYCNHYWENQFKKVYVLEFTRDRKMMSVLCSHKQMDVMFSKGAPESIIARCNKILCNGDGSVVPLTAAGRAELESRFYSFGDETLRCLALAFKTVPHGQQTISYDNENDLTFIGLVGMLDPPREEVRDAMLACMTAGIRVIVVTGDNKSTAESLCRKIGAFDNLVDFSGMSYTASEFERLPAVQQTLALRRMTLFSRVEPSHKRMLVEALQKQNEVVAMTGDGVNDAPALKKADIGIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIFVAAVLGIPDTLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVGEAVVTGWLFFRYLVIGVYVGLATVAGFIWWFVYSDGGPKLTYSELMNFETCALRETTYPCSIFEDRHPSTVAMTVLVVVEMFNALNNLSENQSLLVITPRSNLWLVGSIILTMLLHVLILYVHPLAVLFSVTPLSWAEWTAVLYLSFPVIIIDELLKFLSRNTGMRFRFRLRKADLLPKDRRDK
InterPro domains: - IPR008250: P-type ATPase, A domain superfamily (homologous_superfamily) [124-235]
- IPR001757: P-type ATPase (family) [92-775]
- IPR005782: P-type ATPase, subfamily IIA, SERCA-type (family) [53-845]
- IPR023299: P-type ATPase, cytoplasmic domain N (homologous_superfamily) [352-591]
- IPR036412: HAD-like superfamily (homologous_superfamily) [577-738]
- IPR044492: P-type ATPase, haloacid dehalogenase domain (domain) [326-742]
- IPR018303: P-type ATPase, phosphorylation site (ptm) [347-353]
- IPR023214: HAD superfamily (homologous_superfamily) [342-734]
- IPR006068: Cation-transporting P-type ATPase, C-terminal (domain) [775-973]
- IPR004014: Cation-transporting P-type ATPase, N-terminal (domain) [3-77]
- IPR023298: P-type ATPase, transmembrane domain superfamily (homologous_superfamily) [1-975]
Interaction partners: - Metal tolerance protein 11
- GDT1-like protein 3
- Putative metal tolerance protein C3
- Alpha carbonic anhydrase 2
- Probable metal-nicotianamine transporter YSL4
- Vacuolar cation/proton exchanger 4
- Metal transporter Nramp3
- Metal transporter Nramp2
- Alpha carbonic anhydrase 4
- ATPase GET3C
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005215 (transporter activity), GO:0140657 (ATP-dependent activity), GO:0042626 (ATPase-coupled transmembrane transporter activity), GO:0022857 (transmembrane transporter activity), GO:0005515 (protein binding), GO:0140358 (P-type transmembrane transporter activity), GO:0015075 (monoatomic ion transmembrane transporter activity), GO:0015318 (inorganic molecular entity transmembrane transporter activity), GO:0019829 (ATPase-coupled monoatomic cation transmembrane transporter activity), GO:0022804 (active transmembrane transporter activity), GO:0015399 (primary active transmembrane transporter activity), GO:0022890 (inorganic cation transmembrane transporter activity), GO:0015662 (P-type ion transporter activity), GO:0005388 (P-type calcium transporter activity), GO:0022853 (active monoatomic ion transmembrane transporter activity), GO:0008324 (monoatomic cation transmembrane transporter activity), GO:0046873 (metal ion transmembrane transporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0042221 (response to chemical), GO:0010035 (response to inorganic substance), GO:0010038 (response to metal ion), GO:0046686 (response to cadmium ion)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0030054 (cell junction), GO:0055044 (symplast), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0009506 (plasmodesma), GO:0070161 (anchoring junction), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005911 (cell-cell junction)