Protein: General transcription factor IIH subunit 1 (TFIIH basal transcription factor complex subunit 1) (Q960E8)
Organism: Drosophila melanogaster (Fruit fly)
Function: Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
Subcellular location: Nucleus {ECO:0000250}.
Sequence: MTTSSEDVLLQMGEVRYKKGDGTLYVMNERVAWMAEHRDTVTVSHRYADIKTQKISPEGKPKVQLQVVLHDGNTSTFHFVNRQGQAAMLADRDKVKELLQQLLPNFKRKVDKDLEDKNRILVENPNLLQLYKDLVITKVLTSDEFWATHAKDHALKKMGRSQEIGVSGAFLADIKPQTDGCNGLKYNLTSDVIHCIFKTYPAVKRKHFENVPAKMSEAEFWTKFFQSHYFHRDRLTAGTKDIFTECGKIDDQALKAAVQQGAGDPLLDLKKFEDVPLEEGFGSVAGDRNVVNSGNIVHQNMIKRFNQHSIMVLKTCANVTSAPSTMTNGTNNANGPVSQSAYTNGMNGKGQATATATKSSSDQVDKDEPQSKKQRLMEKIHYVDLGDPILEGDDSANGEKAKSKHFELSKVERYLNGPVQNSMYDNHNDPMSLEEVQYKLVRNSESWLNRNVQRTFICSKAAVNALGELSPGGSMMRGFQEQSAGQLVPNDFQRELRHLYLSLSELLKHFWSCFPPTSEELETKLQRMHETLQRFKMAKLVPFENRAMHELSPLRSSLTQHLNQLLRTANSKFATWKERKLRNTR
InterPro domains: - IPR005607: BSD domain (domain) [102-234]
- IPR013876: TFIIH p62 subunit, N-terminal (domain) [16-100]
- IPR027079: TFIIH subunit Tfb1/GTF2H1 (family) [1-581]
- IPR035925: BSD domain superfamily (homologous_superfamily) [107-238]
- IPR011993: PH-like domain superfamily (homologous_superfamily) [1-110]
Interaction partners: - General transcription factor IIH subunit
- CDK-activating kinase assembly factor MAT1
- General transcription factor IIH subunit 3
- General transcription factor IIH subunit 4
- General transcription and DNA repair factor IIH helicase/translocase subunit XPB
- General transcription and DNA repair factor IIH helicase subunit XPD
- Cyclin-H
- General transcription factor IIE subunit 1
- Transcription initiation factor IIE subunit beta
- Cyclin-dependent kinase 7
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003682 (chromatin binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0010467 (gene expression), GO:0018130 (heterocycle biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0016070 (RNA metabolic process), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006351 (DNA-templated transcription), GO:0006366 (transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0140535 (intracellular protein-containing complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:1902494 (catalytic complex), GO:0140513 (nuclear protein-containing complex), GO:0005667 (transcription regulator complex), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0030880 (RNA polymerase complex), GO:0032806 (carboxy-terminal domain protein kinase complex), GO:0043233 (organelle lumen), GO:0055029 (nuclear DNA-directed RNA polymerase complex), GO:1990234 (transferase complex), GO:0016591 (RNA polymerase II, holoenzyme), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0090575 (RNA polymerase II transcription regulator complex), GO:0000428 (DNA-directed RNA polymerase complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0005675 (transcription factor TFIIH holo complex), GO:0070013 (intracellular organelle lumen), GO:0061695 (transferase complex, transferring phosphorus-containing groups), GO:0031981 (nuclear lumen), GO:1902911 (protein kinase complex), GO:0005634 (nucleus), GO:1902554 (serine/threonine protein kinase complex)