Protein: DNA topoisomerase 3-beta-1 (EC 5.6.2.1) (DNA topoisomerase III beta-1) (O95985)
Organism: Homo sapiens (Human)
Function: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.
Sequence: MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVSHDCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGDAFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRARRPPGKPNPRRPKDKMSALAAYFV
InterPro domains: - IPR006171: TOPRIM domain (domain) [3-154]
- IPR013826: DNA topoisomerase, type IA, central region, subdomain 3 (homologous_superfamily) [297-422]
- IPR000380: DNA topoisomerase, type IA (family) [3-815]
- IPR003601: DNA topoisomerase, type IA, domain 2 (domain) [146-242]
- IPR034144: DNA topoisomerase 3-like, TOPRIM domain (domain) [3-167]
- IPR013824: DNA topoisomerase, type IA, central region, subdomain 1 (homologous_superfamily) [177-593]
- IPR023405: DNA topoisomerase, type IA, core domain (homologous_superfamily) [4-597]
- IPR003602: DNA topoisomerase, type IA, DNA-binding domain (domain) [289-545]
- IPR013497: DNA topoisomerase, type IA, central (domain) [112-593]
- IPR013825: DNA topoisomerase, type IA, central region, subdomain 2 (homologous_superfamily) [236-491]
- IPR023406: DNA topoisomerase, type IA, active site (active_site) [326-340]
- IPR056452: DNA topoisomerase 3-beta, zinc ribbon domain (domain) [616-655]
Interaction partners: - Tudor domain-containing protein 3
- Fanconi anemia group M protein
- RecQ-mediated genome instability protein 1
- RecQ-like DNA helicase BLM
- Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN
- DNA topoisomerase 3-alpha
- RecQ-mediated genome instability protein 2
- E3 ubiquitin-protein ligase TRIM41
- ATP-dependent DNA helicase Q5
- Single-stranded DNA-binding protein, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0000003 (reproduction), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0051321 (meiotic cell cycle), GO:0022402 (cell cycle process), GO:0006807 (nitrogen compound metabolic process), GO:0007049 (cell cycle), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:1903046 (meiotic cell cycle process), GO:0044238 (primary metabolic process), GO:0061982 (meiosis I cell cycle process), GO:0046483 (heterocycle metabolic process), GO:0045132 (meiotic chromosome segregation), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0007131 (reciprocal meiotic recombination), GO:0051726 (regulation of cell cycle), GO:0007059 (chromosome segregation), GO:1901360 (organic cyclic compound metabolic process), GO:0140013 (meiotic nuclear division), GO:0006139 (nucleobase-containing compound metabolic process), GO:0033554 (cellular response to stress), GO:0016043 (cellular component organization), GO:0044260 (cellular macromolecule metabolic process), GO:0010564 (regulation of cell cycle process), GO:0000712 (resolution of meiotic recombination intermediates), GO:0098813 (nuclear chromosome segregation), GO:0006974 (cellular response to DNA damage stimulus), GO:0007127 (meiosis I), GO:0090304 (nucleic acid metabolic process), GO:0006259 (DNA metabolic process), GO:0006996 (organelle organization), GO:0048285 (organelle fission), GO:0006310 (DNA recombination), GO:0006281 (DNA repair), GO:0000280 (nuclear division), GO:0035825 (homologous recombination), GO:0140527 (reciprocal homologous recombination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0030496 (midbody), GO:0045171 (intercellular bridge), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005694 (chromosome), GO:0005634 (nucleus)