Protein: Arginine ADP-riboxanase OspC3 (EC 4.3.99.-) (P0DV36)
Organism: Escherichia coli
Function: ADP-riboxanase effector that inhibits host cell pyroptosis. Acts by mediating arginine ADP-riboxanation of host CASP4/CASP11, blocking CASP4/CASP11 autoprocessing. This prevents CASP4 activation and ability to recognize and cleave GSDMD, thereby inhibiting LPS-induced pyroptosis (By similarity). ADP-riboxanation takes place in two steps: OspC3 first catalyzes ADP-ribosylation of target Arg, and then initiates a deamination to remove one N-omega group (By similarity).
Subcellular location: Secreted {ECO:0000250|UniProtKB:A0A0H2US87}. Host cytoplasm {ECO:0000250|UniProtKB:A0A0H2US87}. Note=Secreted via the type III secretion system (T3SS). {ECO:0000250|UniProtKB:A0A0H2US87}.
Sequence: MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSSSGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSLEHLDKVSRHYISEIIQKVHPLSSDERLLLSIIINSNFNFRHQSNSNLSNNILNIKSFDKMQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI
InterPro domains: - IPR036770: Ankyrin repeat-containing domain superfamily (homologous_superfamily) [357-478]
- IPR010366: Arginine ADP-riboxanase OspC1-4 (family) [191-482]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0016829 (lyase activity), GO:0016840 (carbon-nitrogen lyase activity), GO:0005516 (calmodulin binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016841 (ammonia-lyase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009605 (response to external stimulus), GO:0006950 (response to stress), GO:0019222 (regulation of metabolic process), GO:0009607 (response to biotic stimulus), GO:0051707 (response to other organism), GO:0050794 (regulation of cellular process), GO:0006952 (defense response), GO:0031323 (regulation of cellular metabolic process), GO:0043207 (response to external biotic stimulus), GO:0009617 (response to bacterium), GO:0098542 (defense response to other organism), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:0042742 (defense response to bacterium), GO:0050829 (defense response to Gram-negative bacterium), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)