Protein: Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial (EC 2.3.1.61) (2-oxoglutarate dehydrogenase complex component E2) (OGDC-E2) (Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex) (E2K) (E2o) (PE2o) (Q9N0F1)
Organism: Sus scrofa (Pig)
Function: Dihydrolipoamide succinyltransferase (E2) component of the 2-oxoglutarate dehydrogenase complex. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion (By similarity). A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (By similarity).
Subcellular location: Mitochondrion matrix {ECO:0000305|PubMed:10806400}. Nucleus {ECO:0000250|UniProtKB:P36957}. Note=Mainly localizes in the mitochondrion. A small fraction localizes to the nucleus, where the 2-oxoglutarate dehydrogenase complex is required for histone succinylation. {ECO:0000250|UniProtKB:P36957}.
Sequence: MLSRSRCVSRAFSRSLSAFQKGNCPLGRRSLPGISLCQGPGYPDSRKIVISNSSVLNVRFFRTTAVCKDDVITVKTPAFAESVTEGDVRWEKAVGDTVAEDEVVCEIETDKTSVQVPSPANGVIEALLVPDGGKVEGGTPLFTLRKTGAAPAKAKPAEAPAAAAPKAEPAVSAVPPPPAASIPTQMPPVPSPPQPLTSKPVSAVKPTAAPPVAEPGAVKGLRAEHREKMNRMRQRIAQRLKEAQNTCAMLTTFNEIDMSNIQDMRARHKEAFLKKHNLKLGFMSAFVKASAFALQEQPVVNAVIDDTTKEVVYRDYIDISVAVATPRGLVVPVIRNVETMNYADIERTISELGEKARKNELAIEDMDGGTFTISNGGVFGSLFGTPIINPPQSAILGMHAIVDRPVAVGGKVEIRPMMYVALTYDHRLIDGREAVTFLRKIKAAVEDPRVLLLDL
InterPro domains: - IPR000089: Biotin/lipoyl attachment (domain) [71-145]
- IPR006255: Dihydrolipoamide succinyltransferase (family) [72-454]
- IPR050537: 2-oxoacid dehydrogenase (family) [2-455]
- IPR001078: 2-oxoacid dehydrogenase acyltransferase, catalytic domain (domain) [223-453]
- IPR023213: Chloramphenicol acetyltransferase-like domain superfamily (homologous_superfamily) [217-453]
- IPR003016: 2-oxo acid dehydrogenase, lipoyl-binding site (binding_site) [95-124]
- IPR011053: Single hybrid motif (homologous_superfamily) [71-164]
Interaction partners: - Dihydrolipoyl dehydrogenase, mitochondrial
- 2-oxoglutarate dehydrogenase complex component E1
- 2-oxoglutarate dehydrogenase-like, mitochondrial
- Succinate--CoA ligase subunit beta, mitochondrial
- 2-oxoadipate dehydrogenase complex component E1
- Succinate--CoA ligase subunit alpha, mitochondrial
- 2-oxoisovalerate dehydrogenase subunit alpha
- 3-methyl-2-oxobutanoate dehydrogenase
- Pyruvate dehydrogenase E1 component subunit beta
- Citrate synthase, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0044238 (primary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006793 (phosphorus metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0019637 (organophosphate metabolic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:0019693 (ribose phosphate metabolic process), GO:0009259 (ribonucleotide metabolic process), GO:0034032 (purine nucleoside bisphosphate metabolic process), GO:0009117 (nucleotide metabolic process), GO:0033865 (nucleoside bisphosphate metabolic process), GO:0006163 (purine nucleotide metabolic process), GO:0015936 (coenzyme A metabolic process), GO:0033875 (ribonucleoside bisphosphate metabolic process), GO:0009150 (purine ribonucleotide metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0005739 (mitochondrion), GO:1990234 (transferase complex), GO:0045239 (tricarboxylic acid cycle enzyme complex), GO:0043229 (intracellular organelle), GO:1990204 (oxidoreductase complex), GO:0043227 (membrane-bounded organelle), GO:0045240 (dihydrolipoyl dehydrogenase complex), GO:0043231 (intracellular membrane-bounded organelle)