Protein: SKA complex subunit 1 (Spindle and kinetochore-associated protein 1) (Q9XWS0)
Organism: Caenorhabditis elegans
Function: Component of the ska-1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (By similarity). The ska-1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (By similarity). The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (By similarity). Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules. In the complex, it mediates the interaction with microtubules.
Subcellular location: Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:23085020}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:23085020}.
Sequence: MESFIDRITEETDENFRICRENIPNFETTISPLLNTMKNIKLLLDDAAFDQQKLENNCEETCMNCTKSQLNDFLGIKVEASTIVDVKSQLVPQSKESSALVETNEPNEQGLSSKPKENDIRIVPQITDEEFKTIPKYQLGRLTLEMMNEIVSKMDDFLMKKSKILGKTNKQLTRSDREVLDNWRELEMKARKRLPTTLFFIETDIRPMLQDRLRPSFAKAIPCLRHIRRIREERCGPLTFYYP
InterPro domains: - IPR009829: Spindle and kinetochore-associated protein 1 (family) [2-241]
- IPR042031: SKA1 microtubule binding domain superfamily (homologous_superfamily) [117-243]
Interaction partners: - Spindle and Kinetochore-Associated protein homolog
- Kinetochore protein ndc-80
- Protein zwilch homolog
- Reverse transcriptase domain-containing protein
- Probable deoxycytidylate deaminase
- Kinetochore protein Nuf2 homolog
- Mitotic checkpoint serine/threonine-protein kinase bub-1
- Maternal embryonic leucine zipper kinase
- Kinesin-like protein
- Outer kinetochore KNL1 complex subunit knl-1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0008092 (cytoskeletal protein binding), GO:0015631 (tubulin binding), GO:0008017 (microtubule binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0022402 (cell cycle process), GO:0050794 (regulation of cellular process), GO:0007049 (cell cycle), GO:0051128 (regulation of cellular component organization), GO:0000278 (mitotic cell cycle), GO:0032886 (regulation of microtubule-based process), GO:0007059 (chromosome segregation), GO:0033043 (regulation of organelle organization), GO:0070507 (regulation of microtubule cytoskeleton organization), GO:0051493 (regulation of cytoskeleton organization), GO:0031110 (regulation of microtubule polymerization or depolymerization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005815 (microtubule organizing center), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000940 (outer kinetochore), GO:0098687 (chromosomal region), GO:0099080 (supramolecular complex), GO:0043229 (intracellular organelle), GO:0000775 (chromosome, centromeric region), GO:0000776 (kinetochore), GO:0005813 (centrosome), GO:0043228 (non-membrane-bounded organelle), GO:0000779 (condensed chromosome, centromeric region), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0005694 (chromosome), GO:0000793 (condensed chromosome), GO:0015630 (microtubule cytoskeleton)