Protein: Glutathione gamma-glutamylcysteinyltransferase 2 (EC 2.3.2.15) (Phytochelatin synthase 2) (AtPCS2) (Q9ZWB7)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants.
Sequence: MSMASLYRRSLSPPAIDFASFEGKQIFNEALQKGTMEGFFGLISYFQTQSEPAFCGLASLSMVLNSLSIDPGRKWKGPWRWFDESMLECCEPLEIVKDKGISFGKVVCLAHSSGAKVEAFRTNQSTIDDFRKYVVKCSTSDNCHMISTYHRQVLKQTGTGHFSPIGGYNAERDMALILDVARFKYPPHWVPLKLLWDAMDSIDQSTGRRRGFMLISRPHREPGLLYTLSCKDESWISIAKYLKEDVPRLVSSQHVDTIERILYVVFKSLPANFNQFIKWMAEIRRTEDVNQNLSSEEKSRLKLKQELLKQVQETKLFKHVDKFLSSVYEDNLPYVAAKVYCDGDEILSGYESDESCCKETCVKCIKGLGEEKVTVVAYPSGNDVFTALLLALPPQTWSGIKDQSLLQEMKQLISMVSHPTLLQQEVLHLRRQLEMLKRCQENKEDEELSAPA
InterPro domains: - IPR040409: Phytochelatin synthase (family) [3-432]
- IPR007719: Phytochelatin synthase, N-terminal catalytic domain (domain) [1-220]
- IPR015407: Phytochelatin synthase, C-terminal (domain) [230-433]
- IPR038765: Papain-like cysteine peptidase superfamily (homologous_superfamily) [11-216]
- IPR038156: Phytochelatin synthase, N-terminal domain superfamily (homologous_superfamily) [15-219]
Interaction partners: - Glutathione synthetase, chloroplastic
- TOM1-like protein 2
- Glutamate--cysteine ligase, chloroplastic
- Metallothionein-like protein 2B
- Putative metallothionein-like protein 1B
- Beta-glucosidase 27
- Metallothionein-like protein 1A
- LanC-like protein GCL2
- Thioredoxin domain-containing protein PLP3B
- Zinc finger protein ZAT6
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0005215 (transporter activity), GO:0140657 (ATP-dependent activity), GO:0016740 (transferase activity), GO:0042626 (ATPase-coupled transmembrane transporter activity), GO:0022857 (transmembrane transporter activity), GO:0043167 (ion binding), GO:0140096 (catalytic activity, acting on a protein), GO:0016755 (aminoacyltransferase activity), GO:0016746 (acyltransferase activity), GO:0043169 (cation binding), GO:0140359 (ABC-type transporter activity), GO:0042887 (amide transmembrane transporter activity), GO:0022804 (active transmembrane transporter activity), GO:0015399 (primary active transmembrane transporter activity), GO:1904680 (peptide transmembrane transporter activity), GO:0046872 (metal ion binding), GO:0046914 (transition metal ion binding), GO:0046870 (cadmium ion binding), GO:0005507 (copper ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009605 (response to external stimulus), GO:0051641 (cellular localization), GO:0009058 (biosynthetic process), GO:0042221 (response to chemical), GO:0006807 (nitrogen compound metabolic process), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0033036 (macromolecule localization), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0071554 (cell wall organization or biogenesis), GO:0009607 (response to biotic stimulus), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0051707 (response to other organism), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0043207 (response to external biotic stimulus), GO:0046483 (heterocycle metabolic process), GO:0044248 (cellular catabolic process), GO:0006952 (defense response), GO:0044249 (cellular biosynthetic process), GO:1901700 (response to oxygen-containing compound), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0010033 (response to organic substance), GO:1901135 (carbohydrate derivative metabolic process), GO:0098542 (defense response to other organism), GO:0046685 (response to arsenic-containing substance), GO:0019757 (glycosinolate metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:0010035 (response to inorganic substance), GO:1901360 (organic cyclic compound metabolic process), GO:0033037 (polysaccharide localization), GO:0033554 (cellular response to stress), GO:0006082 (organic acid metabolic process), GO:0009617 (response to bacterium), GO:0006810 (transport), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0043603 (amide metabolic process), GO:1901575 (organic substance catabolic process), GO:0071555 (cell wall organization), GO:0006790 (sulfur compound metabolic process), GO:0010038 (response to metal ion), GO:0009743 (response to carbohydrate), GO:0042545 (cell wall modification), GO:0044270 (cellular nitrogen compound catabolic process), GO:0044273 (sulfur compound catabolic process), GO:0006518 (peptide metabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0043436 (oxoacid metabolic process), GO:0019439 (aromatic compound catabolic process), GO:0042430 (indole-containing compound metabolic process), GO:0046700 (heterocycle catabolic process), GO:0043604 (amide biosynthetic process), GO:0045229 (external encapsulating structure organization), GO:0015698 (inorganic anion transport), GO:0052542 (defense response by callose deposition), GO:0052543 (callose deposition in cell wall), GO:1901566 (organonitrogen compound biosynthetic process), GO:0019760 (glucosinolate metabolic process), GO:0016143 (S-glycoside metabolic process), GO:0052545 (callose localization), GO:1901136 (carbohydrate derivative catabolic process), GO:0042742 (defense response to bacterium), GO:1901361 (organic cyclic compound catabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:1901657 (glycosyl compound metabolic process), GO:0043043 (peptide biosynthetic process), GO:0052386 (cell wall thickening), GO:1901658 (glycosyl compound catabolic process), GO:0046686 (response to cadmium ion), GO:0042436 (indole-containing compound catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0043231 (intracellular membrane-bounded organelle)