Protein: General transcription and DNA repair factor IIH helicase/translocase subunit XPB (TFIIH subunit XPB) (EC 5.6.2.4) (Basic transcription factor 2 89 kDa subunit) (BTF2 p89) (DNA 3'-5' helicase/translocase XPB) (DNA excision repair protein ERCC-3) (DNA repair protein complementing XP-B cells) (TFIIH 89 kDa subunit) (Xeroderma pigmentosum group B-complementing protein) (P49135)
Organism: Mus musculus (Mouse)
Function: ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
Subcellular location: Nucleus.
Sequence: MGKRDRVDRDKKKSKKRQYEEEEEDEDDIPGNESQEAVPSAAGKQVDESSTKVDEYGAKDYRQQMPLKGDHTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYLRKLSKTGVPDGIIQFIKLCTVSYGKVKLVLKHNRYFVESSHPDVIQHLLQDPVIRECRLRNAEGEATELITETFTSKSAISKTAAEGSGGPSTSQGVDAQATSDIPKDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEELQKRCICLEYPLLAEYDFRNDTLNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPARMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEELAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSGQASRRCGTMSSLSGADDTVYMEYHSSRSKASSKHVHPLFKRFRK
InterPro domains: - IPR006935: Helicase/UvrB, N-terminal (domain) [316-472]
- IPR050615: ATP-dependent DNA Helicase (family) [65-762]
- IPR032438: ERCC3/RAD25/XPB helicase, C-terminal domain (domain) [497-734]
- IPR032830: Helicase XPB/Ssl2, N-terminal domain (domain) [76-201]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [264-731]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [313-493]
- IPR001650: Helicase, C-terminal domain-like (domain) [543-703]
- IPR001161: Helicase XPB/Ssl2 (family) [60-780]
Interaction partners: - General transcription factor IIE subunit 1
- General transcription factor IIH subunit 3
- General transcription factor IIH subunit 2
- General transcription and DNA repair factor IIH helicase subunit XPD
- General transcription factor IIH subunit 1
- General transcription factor IIH subunit 4
- General transcription factor IIH subunit 5
- CDK-activating kinase assembly factor MAT1
- General transcription factor IIE subunit 2
- Cyclin-dependent kinase 7
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048869 (cellular developmental process), GO:0009628 (response to abiotic stimulus), GO:0051641 (cellular localization), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009058 (biosynthetic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0033036 (macromolecule localization), GO:0044237 (cellular metabolic process), GO:0008219 (cell death), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0010942 (positive regulation of cell death), GO:0012501 (programmed cell death), GO:1901576 (organic substance biosynthetic process), GO:0006979 (response to oxidative stress), GO:0030154 (cell differentiation), GO:0046483 (heterocycle metabolic process), GO:0009314 (response to radiation), GO:0048731 (system development), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0051726 (regulation of cell cycle), GO:1901360 (organic cyclic compound metabolic process), GO:0010941 (regulation of cell death), GO:0006139 (nucleobase-containing compound metabolic process), GO:0009888 (tissue development), GO:0033554 (cellular response to stress), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0044260 (cellular macromolecule metabolic process), GO:0010564 (regulation of cell cycle process), GO:0009059 (macromolecule biosynthetic process), GO:0009416 (response to light stimulus), GO:1901362 (organic cyclic compound biosynthetic process), GO:0007346 (regulation of mitotic cell cycle), GO:0006974 (cellular response to DNA damage stimulus), GO:0043067 (regulation of programmed cell death), GO:0019438 (aromatic compound biosynthetic process), GO:0043068 (positive regulation of programmed cell death), GO:0030855 (epithelial cell differentiation), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0022008 (neurogenesis), GO:0007399 (nervous system development), GO:0090304 (nucleic acid metabolic process), GO:0008544 (epidermis development), GO:0018130 (heterocycle biosynthetic process), GO:1901987 (regulation of cell cycle phase transition), GO:0006915 (apoptotic process), GO:0006259 (DNA metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0008104 (protein localization), GO:0060429 (epithelium development), GO:0006996 (organelle organization), GO:0010467 (gene expression), GO:0030182 (neuron differentiation), GO:0043065 (positive regulation of apoptotic process), GO:0016070 (RNA metabolic process), GO:0042981 (regulation of apoptotic process), GO:0006281 (DNA repair), GO:0051276 (chromosome organization), GO:0006265 (DNA topological change), GO:0035315 (hair cell differentiation), GO:1901990 (regulation of mitotic cell cycle phase transition), GO:0009411 (response to UV), GO:0048699 (generation of neurons), GO:0009913 (epidermal cell differentiation), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006289 (nucleotide-excision repair), GO:0071103 (DNA conformation change), GO:0006351 (DNA-templated transcription), GO:0006354 (DNA-templated transcription elongation), GO:0006368 (transcription elongation by RNA polymerase II), GO:0032392 (DNA geometric change), GO:0006283 (transcription-coupled nucleotide-excision repair), GO:0006366 (transcription by RNA polymerase II), GO:0032508 (DNA duplex unwinding)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)