Protein: Zona pellucida sperm-binding protein 2 (Zona pellucida glycoprotein 2) (Zp-2) (Zona pellucida protein A) [Cleaved into: Processed zona pellucida sperm-binding protein 2] (P47983)
Organism: Canis lupus familiaris (Dog) (Canis familiaris)
Function: Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.
Subcellular location: [Processed zona pellucida sperm-binding protein 2]: Zona pellucida {ECO:0000250|UniProtKB:P20239}.; SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P20239}; Single-pass type I membrane protein {ECO:0000255}.
Sequence: MACKQKGDSGSPSSRFSADWSTYRSLSLFFILVTSVNSVGVMQLVNPIFPGTVICHENKMTVEFPRDLGTKKWHASVVDPFSFELLNCTSILDPEKLTLKAPYETCSRRVLGQHQMAIRLTDNNAASRHKAFMYQISCPVMQTEETHEHAGSTICTKDSMSFTFNIIPGMADENTNPSGGKWMMEVDDAKAQNLTLREALMQGYNFLFDSHRLSVQVSFNATGVTHYMQGNSHLYTVPLKLIHTSPGQKIILTTRVLCMSDPVTCNATHMTLTIPEFPGKLQSVRFENTNFRVSQLHNHGIDKEELNGLRLHFSKSLLKMNSSEKCLLYQFYLASLKLTFAFERDTVSTVVYPECVCEPPVTIVTGDLCTQDGFMDVKVYSHQTKPALNLDTLRVGDSSCQPTFKAPSQGLTLFHIPLNGCGTRLKFKGDTVIYENEIHALWTDLPPSTISRDSEFRMTVKCHYSRDDLLINTNVQSLPPPVASVRPGPLALILQTYPDKSYLRPYGDKEYPVVRYLRQPIYLEVKVLNRADPNIKLVLDDCWATPTMDPASLPQWNIVMDGCEYNLDNYRTTFHPVGSSVTYPTHYQRFDVKTFAFISEAQVLSSLVYFHCTALICNRLSPDSPLCSVTCPVSSRHRRATGSTEEEKMIVSLPGPILLLADSSSLRDGVDSKGHRAAGYVAFKTVVAVAALAGLVAALGLIIYLRKKRTMVLNH
InterPro domains: - IPR042235: Zona pellucida, ZP-C domain (homologous_superfamily) [489-662]
- IPR051148: Zona Pellucida Domain Glycoprotein (family) [23-709]
- IPR057637: Zona pellucida sperm-binding protein 2, first Ig-like domain (domain) [51-123]
- IPR055356: ZP-N domain (domain) [369-465]
- IPR001507: Zona pellucida domain (domain) [368-634]
- IPR017977: Zona pellucida domain, conserved site (conserved_site) [523-563]
- IPR048290: Zona pellucida domain, chordate-type (domain) [391-621]
- IPR055355: ZP-C domain (domain) [488-628]
- IPR057638: Zona pellucida sperm-binding protein 2, second Ig-like domain (domain) [141-263]
- IPR057636: Zona pellucida sperm-binding protein 2, third Ig-like domain (domain) [265-357]
Interaction partners: - Zona pellucida sperm-binding protein 3
- Growth differentiation factor 9.
- Zona pellucida glycoprotein 4.
- Folliculogenesis specific bHLH transcription factor.
- Oviductal glycoprotein 1
- Bone morphogenetic protein 15.
- NLR family pyrin domain containing 5.
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0022414 (reproductive process), GO:0002376 (immune system process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0000003 (reproduction), GO:0008037 (cell recognition), GO:0019953 (sexual reproduction), GO:0050793 (regulation of developmental process), GO:0002252 (immune effector process), GO:2000243 (positive regulation of reproductive process), GO:0009566 (fertilization), GO:0051094 (positive regulation of developmental process), GO:2000241 (regulation of reproductive process), GO:0006955 (immune response), GO:0035036 (sperm-egg recognition), GO:0007339 (binding of sperm to zona pellucida), GO:0002443 (leukocyte mediated immunity), GO:0009988 (cell-cell recognition), GO:0002250 (adaptive immune response), GO:0007338 (single fertilization), GO:0006959 (humoral immune response), GO:0002455 (humoral immune response mediated by circulating immunoglobulin), GO:0002449 (lymphocyte mediated immunity), GO:0002460 (adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains), GO:0019724 (B cell mediated immunity), GO:0016064 (immunoglobulin mediated immune response)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0030312 (external encapsulating structure), GO:0031012 (extracellular matrix), GO:0062023 (collagen-containing extracellular matrix)