Protein: Sterol O-acyltransferase 1 (EC 2.3.1.26) (Acyl-coenzyme A:cholesterol acyltransferase 1) (ACAT-1) (Cholesterol acyltransferase 1) (P35610)
Organism: Homo sapiens (Human)
Function: Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. Utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) preferentially as susbstrate: shows a higher activity towards an acyl-CoA substrate with a double bond at the delta-9 position (9Z) than towards saturated acyl-CoA or an unsaturated acyl-CoA with a double bond at the delta-7 (7Z) or delta-11 (11Z) positions.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:10438503, ECO:0000269|PubMed:16154994}; Multi-pass membrane protein {ECO:0000269|PubMed:32433613, ECO:0000269|PubMed:32433614}.
Sequence: MVGEEKMSLRNRLSKSRENPEEDEDQRNPAKESLETPSNGRIDIKQLIAKKIKLTAEAEELKPFFMKEVGSHFDDFVTNLIEKSASLDNGGCALTTFSVLEGEKNNHRAKDLRAPPEQGKIFIARRSLLDELLEVDHIRTIYHMFIALLILFILSTLVVDYIDEGRLVLEFSLLSYAFGKFPTVVWTWWIMFLSTFSVPYFLFQHWATGYSKSSHPLIRSLFHGFLFMIFQIGVLGFGPTYVVLAYTLPPASRFIIIFEQIRFVMKAHSFVRENVPRVLNSAKEKSSTVPIPTVNQYLYFLFAPTLIYRDSYPRNPTVRWGYVAMKFAQVFGCFFYVYYIFERLCAPLFRNIKQEPFSARVLVLCVFNSILPGVLILFLTFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYYRTWNVVVHDWLYYYAYKDFLWFFSKRFKSAAMLAVFAVSAVVHEYALAVCLSFFYPVLFVLFMFFGMAFNFIVNDSRKKPIWNVLMWTSLFLGNGVLLCFYSQEWYARQHCPLKNPTFLDYVRPRSWTCRYVF
InterPro domains: - IPR014371: Sterol O-acyltransferase, ACAT/DAG/ARE types (family) [23-530]
- IPR030687: Sterol O-acyltransferase, metazoa (family) [1-548]
- IPR004299: Membrane bound O-acyl transferase, MBOAT (family) [171-520]
Interaction partners: - 7-dehydrocholesterol reductase
- Delta
- Sterol O-acyltransferase 2
- Cholesterol 24-hydroxylase
- Cholesterol 25-hydroxylase
- Cytochrome P450 7A1
- Sterol 26-hydroxylase, mitochondrial
- Cholesterol side-chain cleavage enzyme, mitochondrial
- Steryl-sulfatase
- Sulfotransferase 2B1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0005488 (binding), GO:0036094 (small molecule binding), GO:0008289 (lipid binding), GO:1901681 (sulfur compound binding), GO:0097159 (organic cyclic compound binding), GO:0016740 (transferase activity), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0033218 (amide binding), GO:0097367 (carbohydrate derivative binding), GO:0005515 (protein binding), GO:0043178 (alcohol binding), GO:0005496 (steroid binding), GO:1901265 (nucleoside phosphate binding), GO:1901567 (fatty acid derivative binding), GO:0043168 (anion binding), GO:0120227 (acyl-CoA binding), GO:0000166 (nucleotide binding), GO:0016746 (acyltransferase activity), GO:0032553 (ribonucleotide binding), GO:0000062 (fatty-acyl-CoA binding), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups), GO:0015485 (cholesterol binding), GO:0017076 (purine nucleotide binding), GO:0032555 (purine ribonucleotide binding), GO:0032934 (sterol binding), GO:0008374 (O-acyltransferase activity), GO:0032559 (adenyl ribonucleotide binding), GO:0030554 (adenyl nucleotide binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0071704 (organic substance metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:0006629 (lipid metabolic process), GO:0006066 (alcohol metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0016125 (sterol metabolic process), GO:1902652 (secondary alcohol metabolic process), GO:0008202 (steroid metabolic process), GO:0008203 (cholesterol metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031984 (organelle subcompartment), GO:0098862 (cluster of actin-based cell projections), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0031090 (organelle membrane), GO:0005783 (endoplasmic reticulum), GO:0043229 (intracellular organelle), GO:0005903 (brush border), GO:0043227 (membrane-bounded organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0043231 (intracellular membrane-bounded organelle), GO:0005789 (endoplasmic reticulum membrane)