Protein: Sterol 14-alpha demethylase (EC 1.14.14.154) (Cytochrome P450 monooxygenase 51A) (Ergosterol biosynthesis protein cyp51A) (B8N2C8)
Organism: Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167)
Function: Cytochrome P450 monooxygenase involved in the biosynthesis of ergosterol (By similarity). The existence of several duplicated sterol 14-alpha demethylase genes could be a good strategy to modulate the composition and fluidity of the cell membrane (By similarity). Catalyzes C14-demethylation of erburicol to produce 4,4,24-trimethyl cholesta-8,24(28)-dien-3-beta-ol (By similarity). Shows a slight substrate preference toward eburicol over obtusifoliol (By similarity). As a target of azole drugs, plays a crucial role in azole susceptibility.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000255}.
Sequence: MIFSRSMASFTLVSAYAAAGLLAIIVLNLLRQLLFRNKTDPPLVFHWIPFLGSTVTYGMDPYAFFFSCRQKYGDIFTFILLGRKITVYLGIQGNEFILNGKLKDVNAEEIYSPLTTPVFGSDIVYDCPNSKLMEQKKFIKFGLTQAALESHVPLIEKEVLDYLKTSPNFKGTSGRVEITDAMAEITIFTAGRALQGEEVRKKLTAEFADLYHDLDRGFTPINFMLPWAPLPRNRKRDAAHARMREIYMDIINERRKNPDRETSDMIWNLMHCTYKNGQPLPDKEIAHMMITLLMAGQHSSSSISSWIMLRLASEPAVMEELYQEQITKLSPDGRTLPPLQYRDLDLLPLHQNLIKETLRLHLSIHSLMRKVKNPMPVPGTPYVVPADHVLLASPGVTALSDEYFPNASRWDPHRWENRVEKEDEEDIVDYGYGTVSKGTSSPYLPFGAGRHRCIGEKFAYVNLGVIVATMARHMKLFNVDGKKGVPATDYSSMFSGPSKPAIIGWERRFPEKS
InterPro domains: - IPR036396: Cytochrome P450 superfamily (homologous_superfamily) [33-513]
- IPR017972: Cytochrome P450, conserved site (conserved_site) [446-455]
- IPR001128: Cytochrome P450 (family) [44-502]
- IPR050529: Cytochrome P450, sterol 14-alpha demethylase (family) [15-500]
- IPR002403: Cytochrome P450, E-class, group IV (family) [41-471]
Interaction partners: - Terpene cyclase/mutase family member
- Terpene cyclase/mutase family member
- Terpene cyclase/mutase family member
- Delta
- Delta
- Sterol 14-alpha demethylase
- Sterol 14-alpha demethylase
- Farnesyl-diphosphate farnesyltransferase, putative.
- 3-hydroxy-3-methylglutaryl coenzyme A synthase
- 3-hydroxy-3-methylglutaryl coenzyme A synthase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0032451 (demethylase activity), GO:0016491 (oxidoreductase activity), GO:0004497 (monooxygenase activity), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0016709 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006066 (alcohol metabolic process), GO:0044107 (cellular alcohol metabolic process), GO:0016125 (sterol metabolic process), GO:1902652 (secondary alcohol metabolic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0008204 (ergosterol metabolic process), GO:0008202 (steroid metabolic process), GO:0097384 (cellular lipid biosynthetic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:0044108 (cellular alcohol biosynthetic process), GO:0046165 (alcohol biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0016128 (phytosteroid metabolic process), GO:0006696 (ergosterol biosynthetic process), GO:0006694 (steroid biosynthetic process), GO:0016126 (sterol biosynthetic process), GO:0016129 (phytosteroid biosynthetic process), GO:1902653 (secondary alcohol biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)