Protein: Phosphatidylinositol 4-kinase pik1 (PI4-kinase) (PtdIns-4-kinase) (EC 2.7.1.67) (Q10366)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Acts on phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol 1,4,5,-trisphosphate. PIK1 is part of a nuclear phosphoinositide cycle and could control cytokinesis through the actin cytoskeleton (By similarity).
Subcellular location: Golgi apparatus {ECO:0000269|PubMed:16823372}. Nucleus {ECO:0000250}.
Sequence: MPSSNSGNELLLRFFESAHFTSQLCVAYLSRYPNNIGIHHFLCEKLATFPYEEIEFFIPQLIHLVLNKDSESVALEEFIISQCEQNTQCALITFWYLQAHMVDLGLQPHSSCFKICKRLYNRIQILVFMSSSSLIQSQQKISENVTPALILSGIMLGGVCVPELLKKAGPIAIAQGRKAPRQDPDESDVDVLRRLSTEPRYSLDVLRSSLNNSIVEQHSEVSLRLKAPELTRTHSYQSSATLSIDEQRRVLRSNYFQQEIQFLFALQDISIRLIIVPRQARLSSLRAELALLNNNLPADVNIPLLRSYHKEVSHKIVRIDPKEATILNSAERVPYLIMVEVLSGELSFEPQSKKNKAKVQKIVSHKNQRKRWFDLTDVDPYTNLQDSTDNDISESESEGGDLSMSPLIKGLVPDTLSLSKSFSSFGNVSLQVPSSHRDTDVVLLSGRHSDSDGNGALKRSKIYASEITARMRAAATMLSQLDAEGSRRPKAETERIKNSIILDMQRLEEERLNEPSIYPVSVDISCAEDLRFGKETQKAERKGDRDDPSAATFQEDWYAKKERIRKSSPYGHYPNWDLVSVIVKTGADLRQETFACQLIYAFQRVWLECKEKVWVRRMKILVTGDNSGLIETITNAISVHSIKKNLTKQLREAELAQGKIAGKNVVTLKDYFIKQFGDPNSSRYRQAQTNFLQSLVAYSIISYLLQLKDRHNGNVLIDSEGHIIHIDFGFLLTNTPGNVGFESAPFKLTADYLEILDDRFDEYRSLMKAAFKSVRKNADQIILLVEVMQNNSTMPCFRAGENTSAQLLQRFQLQLGDKECDDFVDLLIQKANCSVWTRLYDLFQNITNGIY
InterPro domains: - IPR016024: Armadillo-type fold (homologous_superfamily) [20-101]
- IPR021601: Phosphatidylinositol 4-kinase, Pik1, fungi (family) [110-160]
- IPR015433: Phosphatidylinositol 3-/4-kinase (family) [14-851]
- IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [254-846]
- IPR018936: Phosphatidylinositol 3-/4-kinase, conserved site (conserved_site) [583-714]
- IPR049160: PI4KB/PIK1, accessory domain (domain) [26-97]
- IPR036940: Phosphatidylinositol 3-/4-kinase, catalytic domain superfamily (homologous_superfamily) [659-849]
- IPR000403: Phosphatidylinositol 3-/4-kinase, catalytic domain (domain) [558-849]
- IPR001263: Phosphoinositide 3-kinase, accessory (PIK) domain (domain) [1-123]
- IPR057754: Phosphatidylinositol 4-kinase beta/PIK1, catalytic domain (domain) [549-851]
Interaction partners: - Phosphatidylinositol 4-kinase lsb6
- Calcium-binding protein NCS-1
- Phosphatidylinositol 4-phosphate 5-kinase its3
- GTP-binding protein ypt3
- CDP-diacylglycerol--inositol 3-phosphatidyltransferase
- Phosphatidylinositol 4-kinase stt4
- 1-phosphatidylinositol 3-phosphate 5-kinase fab1
- Uncharacterized protein C19F5.03
- Uncharacterized protein C3C7.01c
- Phosphatidylinositol 3-kinase pik3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0016301 (kinase activity), GO:0052742 (phosphatidylinositol kinase activity), GO:0004430 (1-phosphatidylinositol 4-kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044238 (primary metabolic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0019637 (organophosphate metabolic process), GO:0006793 (phosphorus metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0046486 (glycerolipid metabolic process), GO:0008610 (lipid biosynthetic process), GO:0006644 (phospholipid metabolic process), GO:0045017 (glycerolipid biosynthetic process), GO:0090407 (organophosphate biosynthetic process), GO:0006650 (glycerophospholipid metabolic process), GO:0046474 (glycerophospholipid biosynthetic process), GO:0008654 (phospholipid biosynthetic process), GO:0006661 (phosphatidylinositol biosynthetic process), GO:0046488 (phosphatidylinositol metabolic process), GO:0046854 (phosphatidylinositol phosphate biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0031090 (organelle membrane), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0000139 (Golgi membrane)