Protein: L-2-amino-4-chloropent-4-enoate dechlorinase/desaturase (EC 4.5.1.-) (4-chloro-allyl-L-glycine acetylenase) (L-propargylglycine synthase) (G8XHD7)
Organism: Streptantibioticus cattleyicolor (strain ATCC 35852 / DSM 46488 / JCM 4925 / NBRC 14057 / NRRL 8057) (Streptomyces cattleya)
Function: Involved in the biosynthesis of terminal alkyne-containing amino acids such as L-propargylglycine (Pra) and L-beta-ethynylserine, that are produced as antibiotics by S.cattleya. Catalyzes gamma-elimination of chloride from 4-chloro-allyl-L-glycine (also named L-2-amino-4-chloropent-4-enoate), followed by an isomerization, to form the terminal-alkyne product L-propargylglycine.
Sequence: MSAGSPAEPRPVPGSVHSVSVSIPDVRSVIGFESGDPATLRRIAWGYPRFRTHPYVARVAALVAGAVGGRAQDLVLTRSVRAAEAAAAYAGLAPGAVFEASGVRGVRVAEDDPALAAVRGHVQHTGAHLTSREAEDVLLEAGLIEARQAEEAVSEEPAEAVRSALATAYGAGDPADVSLHNSGMNAVAAAVAAVTDLQRPAGRRRWIQLGWIFFDTMSLLDKRLFGTDHVTVPDPFDLAALSRVVAAHPGQLAGIIAELPSNPSLRCPDVPALREIADRAGCALVLDATIATPHNVDVLGYADVVCESLTKYATGSADVLMGAAVVGSASPWAAQLREGLRRFGDVPYHRDAARVAARIRDYGDRMKRVNAGAVALAGFLERQSAVRAVSWPYDAASQANYRKVERLSDAPGGLLMVDLRVPLERVYDRLAVAKGPSFGAEFTMASPQIFIAHFDLLSTPEGRAELRSRGLHRDMLRISVGVEDPELIAEVFRDAFDGAG
InterPro domains: - IPR055001: L-2-amino-4-chloropent-4-enoate dechlorinase/desaturase (family) [10-498]
- IPR015421: Pyridoxal phosphate-dependent transferase, major domain (homologous_superfamily) [109-360]
- IPR051750: Trans-sulfuration enzymes (family) [157-498]
- IPR015424: Pyridoxal phosphate-dependent transferase (homologous_superfamily) [156-497]
- IPR015422: Pyridoxal phosphate-dependent transferase, small domain (homologous_superfamily) [365-500]
- IPR000277: Cys/Met metabolism, pyridoxal phosphate-dependent enzyme (family) [176-496]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016829 (lyase activity), GO:0016835 (carbon-oxygen lyase activity), GO:0016836 (hydro-lyase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0006790 (sulfur compound metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0000096 (sulfur amino acid metabolic process), GO:0044272 (sulfur compound biosynthetic process), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0009066 (aspartate family amino acid metabolic process), GO:0006534 (cysteine metabolic process), GO:0000097 (sulfur amino acid biosynthetic process), GO:0009069 (serine family amino acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0006555 (methionine metabolic process), GO:0009070 (serine family amino acid biosynthetic process), GO:0009092 (homoserine metabolic process), GO:0019344 (cysteine biosynthetic process), GO:0009086 (methionine biosynthetic process), GO:0009067 (aspartate family amino acid biosynthetic process), GO:0071265 (L-methionine biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)