Protein: Baculoviral IAP repeat-containing protein 7 (EC 2.3.2.27) (Livin) (RING-type E3 ubiquitin transferase BIRC7) [Cleaved into: Baculoviral IAP repeat-containing protein 7 30 kDa subunit (Truncated livin) (p30-Livin) (tLivin)] (A2AWP0)
Organism: Mus musculus (Mouse)
Function: Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and MAP3K7/TAK1. In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9.
Subcellular location: Nucleus {ECO:0000250|UniProtKB:Q96CA5}. Cytoplasm {ECO:0000250|UniProtKB:Q96CA5}. Golgi apparatus {ECO:0000250|UniProtKB:Q96CA5}. Note=Nuclear, and in a filamentous pattern throughout the cytoplasm. Full-length livin is detected exclusively cytoplasm, whereas the truncated form (tLivin) is found in the peri-nuclear region with marked localization to the Golgi apparatus; the accumulation of tLivin in the nucleus shows positive correlation with the increase in apoptosis. {ECO:0000250|UniProtKB:Q96CA5}.
Sequence: MFSPADLFRAAVFSMGPESRARDSVRGPELSHREDGSGRTQEQDKPHCPCNHVLGQDCLDGQILGQLRPLSEEEESSGAAFLGEPAFPEMDSEDLRLASFYDWPSTAGIQPEPLAAAGFFHTGQQDKVRCFFCYGGLQSWERGDDPWTEHARWFPRCQFLLRSKGRDFVERIQTYTPLLGSWDQREEPEDAVSATPSAPAHGSPELLRSRRETQPEDVSEPGAKDVQEQLRQLQEERRCKVCLDRAVSIVFVPCGHFVCTECAPNLQLCPICRVPICSCVRTFLS
InterPro domains: - IPR017907: Zinc finger, RING-type, conserved site (conserved_site) [254-263]
- IPR001370: BIR repeat (repeat) [91-162]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [216-285]
- IPR050784: Inhibitor of Apoptosis (family) [3-227]
- IPR001841: Zinc finger, RING-type (domain) [239-273]
Interaction partners: - Caspase-9
- Caspase-7
- Caspase-3
- Diablo IAP-binding mitochondrial protein
- Ubiquitin-ribosomal protein eS31 fusion protein
- Ubiquitin-conjugating enzyme E2 D2
- Serine protease HTRA2, mitochondrial
- E3 ubiquitin-protein ligase RNF4
- Polyubiquitin-B
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0030234 (enzyme regulator activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0140678 (molecular function inhibitor activity), GO:0019787 (ubiquitin-like protein transferase activity), GO:0061134 (peptidase regulator activity), GO:0004857 (enzyme inhibitor activity), GO:0061135 (endopeptidase regulator activity), GO:0004842 (ubiquitin-protein transferase activity), GO:0030414 (peptidase inhibitor activity), GO:0004866 (endopeptidase inhibitor activity), GO:0004869 (cysteine-type endopeptidase inhibitor activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0051716 (cellular response to stimulus), GO:0065009 (regulation of molecular function), GO:0048523 (negative regulation of cellular process), GO:0009892 (negative regulation of metabolic process), GO:0048522 (positive regulation of cellular process), GO:0050790 (regulation of catalytic activity), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0042127 (regulation of cell population proliferation), GO:0060548 (negative regulation of cell death), GO:0010941 (regulation of cell death), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0044092 (negative regulation of molecular function), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0010033 (response to organic substance), GO:0043086 (negative regulation of catalytic activity), GO:0034097 (response to cytokine), GO:0010562 (positive regulation of phosphorus metabolic process), GO:0051246 (regulation of protein metabolic process), GO:0043069 (negative regulation of programmed cell death), GO:0043067 (regulation of programmed cell death), GO:0071310 (cellular response to organic substance), GO:0051336 (regulation of hydrolase activity), GO:0051248 (negative regulation of protein metabolic process), GO:0010563 (negative regulation of phosphorus metabolic process), GO:0051247 (positive regulation of protein metabolic process), GO:0051174 (regulation of phosphorus metabolic process), GO:0042981 (regulation of apoptotic process), GO:0051346 (negative regulation of hydrolase activity), GO:0030162 (regulation of proteolysis), GO:0031399 (regulation of protein modification process), GO:0052547 (regulation of peptidase activity), GO:0045937 (positive regulation of phosphate metabolic process), GO:0043066 (negative regulation of apoptotic process), GO:0045861 (negative regulation of proteolysis), GO:0031401 (positive regulation of protein modification process), GO:0045936 (negative regulation of phosphate metabolic process), GO:0071345 (cellular response to cytokine stimulus), GO:0019220 (regulation of phosphate metabolic process), GO:0042325 (regulation of phosphorylation), GO:0001933 (negative regulation of protein phosphorylation), GO:0001932 (regulation of protein phosphorylation), GO:0052548 (regulation of endopeptidase activity), GO:0010466 (negative regulation of peptidase activity), GO:0043154 (negative regulation of cysteine-type endopeptidase activity involved in apoptotic process), GO:0042327 (positive regulation of phosphorylation), GO:0001934 (positive regulation of protein phosphorylation), GO:2000116 (regulation of cysteine-type endopeptidase activity), GO:0033138 (positive regulation of peptidyl-serine phosphorylation), GO:0033135 (regulation of peptidyl-serine phosphorylation), GO:0010951 (negative regulation of endopeptidase activity), GO:2000117 (negative regulation of cysteine-type endopeptidase activity), GO:0043281 (regulation of cysteine-type endopeptidase activity involved in apoptotic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0031974 (membrane-enclosed lumen), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0043233 (organelle lumen), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus)