Protein: Transcription initiation factor TFIID subunit 1 (EC 2.3.1.48) (EC 2.7.11.1) (Cell cycle gene 1 protein) (TBP-associated factor 250 kDa) (p250) (Transcription initiation factor TFIID 250 kDa subunit) (TAF(II)250) (TAFII-250) (TAFII250) (Q80UV9)
Organism: Mus musculus (Mouse)
Function: The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (By similarity). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (By similarity). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (By similarity). TAF1 is the largest component and core scaffold of the TFIID complex, involved in nucleating complex assembly. TAF1 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF2 (By similarity). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors (By similarity). Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53 (By similarity). Phosphorylates GTF2A1 and GTF2F1 on Ser residues (By similarity). Possesses DNA-binding activity (By similarity). Exhibits histone acetyltransferase activity towards histones H3 and H4 (By similarity). Essential for progression of the G1 phase of the cell cycle (By similarity).
Subcellular location: Nucleus {ECO:0000250|UniProtKB:P21675}.
Sequence: MGPGWAGLLQDKGGGSPSVVMSDTDSDEESAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELSGSDGALVNDEGWIRSREDAVDYSDINEVAEDESRRYQQTMGSLQPLCHTDYDEDDYDADCEDIDCKLMPPPPPPPGPLKKEKDQDDITGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQDAAQSESKDGQLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEGQVQEEECSVELEVNQKSLWNYDYAPPPLPDQCLSDDEITMMAPVESKFSQSTGDTDKVMDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKMKKTEHESTIKCNIMKKLRKLEENSGVDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFTATLDDDKPWYSIFPIDNEDLVYGRWEDNIIWDAQNMPRILEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPESDFLIIRTRQGYFIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAAAGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKATVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCHQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDNNTSLSVSRDASVYQDESNLSVLDIPSATSEKQLTQEGGDGDGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFFAIQLSESGSDSDVESGSLRPKQPRVLQENTRMGMENEESMMSYEGDGGDASRGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDTDLDSDE
InterPro domains: - IPR011177: Transcription initiation factor TFIID subunit 1, animal (family) [18-1891]
- IPR041670: Zinc knuckle (domain) [1282-1323]
- IPR009067: TAFII-230 TBP-binding (domain) [25-86]
- IPR018359: Bromodomain, conserved site (conserved_site) [1546-1603]
- IPR036741: TAFII-230 TBP-binding domain superfamily (homologous_superfamily) [29-87]
- IPR036427: Bromodomain-like superfamily (homologous_superfamily) [1360-1650]
- IPR040240: Transcription initiation factor TFIID subunit 1 (family) [21-1644]
- IPR022591: Transcription initiation factor TFIID subunit 1, histone acetyltransferase domain (domain) [586-1048]
- IPR001487: Bromodomain (domain) [1399-1630]
Interaction partners: - Transcription initiation factor TFIID subunit 2
- TATA-box-binding protein
- Transcription initiation factor TFIID subunit 10
- Transcription initiation factor TFIID subunit 12
- Transcription initiation factor TFIID subunit 6
- Transcription initiation factor TFIID subunit 11
- Transcription initiation factor TFIID subunit 13
- Transcription initiation factor TFIID subunit 5
- Transcription initiation factor TFIID subunit 8
- TATA-box binding protein associated factor 4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140223 (general transcription initiation factor activity), GO:0098772 (molecular function regulator activity), GO:0005488 (binding), GO:0030234 (enzyme regulator activity), GO:0140677 (molecular function activator activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0008134 (transcription factor binding), GO:0042393 (histone binding), GO:0019787 (ubiquitin-like protein transferase activity), GO:0004672 (protein kinase activity), GO:0008047 (enzyme activator activity), GO:0140030 (modification-dependent protein binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0046983 (protein dimerization activity), GO:0001098 (basal transcription machinery binding), GO:0016746 (acyltransferase activity), GO:0140416 (transcription regulator inhibitor activity), GO:0004402 (histone acetyltransferase activity), GO:0004674 (protein serine/threonine kinase activity), GO:0004842 (ubiquitin-protein transferase activity), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups), GO:0016301 (kinase activity), GO:0140033 (acetylation-dependent protein binding), GO:0140297 (DNA-binding transcription factor binding), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0140296 (general transcription initiation factor binding), GO:0061650 (ubiquitin-like protein conjugating enzyme activity), GO:0046982 (protein heterodimerization activity), GO:0070577 (lysine-acetylated histone binding), GO:0016407 (acetyltransferase activity), GO:0061629 (RNA polymerase II-specific DNA-binding transcription factor binding), GO:0017025 (TBP-class protein binding), GO:0016410 (N-acyltransferase activity), GO:0016922 (nuclear receptor binding), GO:0008080 (N-acetyltransferase activity), GO:0001046 (core promoter sequence-specific DNA binding), GO:0000977 (RNA polymerase II transcription regulatory region sequence-specific DNA binding), GO:0000979 (RNA polymerase II core promoter sequence-specific DNA binding), GO:0034212 (peptide N-acetyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0042221 (response to chemical), GO:0009410 (response to xenobiotic stimulus)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0030054 (cell junction), GO:0098794 (postsynapse), GO:0140535 (intracellular protein-containing complex), GO:1902494 (catalytic complex), GO:0140513 (nuclear protein-containing complex), GO:0005667 (transcription regulator complex), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0030880 (RNA polymerase complex), GO:1990234 (transferase complex), GO:0043229 (intracellular organelle), GO:0045202 (synapse), GO:0090575 (RNA polymerase II transcription regulator complex), GO:0043228 (non-membrane-bounded organelle), GO:0043233 (organelle lumen), GO:0055029 (nuclear DNA-directed RNA polymerase complex), GO:0016591 (RNA polymerase II, holoenzyme), GO:0043227 (membrane-bounded organelle), GO:0000428 (DNA-directed RNA polymerase complex), GO:0005669 (transcription factor TFIID complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0061695 (transferase complex, transferring phosphorus-containing groups), GO:0070013 (intracellular organelle lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus), GO:0031981 (nuclear lumen)