Protein: Equilibrative nucleoside transporter 2 (O54699)
Organism: Rattus norvegicus (Rat)
Function: Bidirectional uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis. Functions as a Na(+)-independent, passive transporter (By similarity). Involved in the transport of nucleosides such as inosine, adenosine, uridine, thymidine, cytidine and guanosine. Also able to transport purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil). Involved in nucleoside transport at basolateral membrane of kidney cells, allowing liver absorption of nucleoside metabolites (By similarity). Mediates apical nucleoside uptake into Sertoli cells, thereby regulating the transport of nucleosides in testis across the blood-testis-barrier. Mediates both the influx and efflux of hypoxanthine in skeletal muscle microvascular endothelial cells to control the amount of intracellular hypoxanthine available for xanthine oxidase- mediated ROS production
Subcellular location: Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Note=Localized to the apical membrane of Sertoli cells
Sequence: MAHGNAPRDSYHLVGISFFILGLGTLLPWNFFITAIPYFQGRLAGTNSSAETPSTNHTSPTDTFNFNNWVTLLSQLPLLLFTLLNSFLYQCIPESVRILGSLLAILLLFALTAALVKVDLSPGLFFSITMASVWFINSFCAVLQGSLFGQLGTMPSTYSTLFLSGQGLAGIFAALAMLTSLASGVDPQTSALGYFITPCVGILLSIICYLSLPHLKFARYYLTKKPQAPVQELETKAELLGADEKNGIPVSPQQAGPTLDLDPEKELELGLEEPQKPGKPSVFVVFRKIWLTALCLVLVFTVTLSVFPAITAMVTTSSNSPGKWSQFFNPICCFLLFNVMDWLGRSLTSYFLWPDEDSQLLPLLVCLRFLFVPLFMLCHVPQRARLPIIFWQDAYFITFMLLFAISNGYFVSLTMCLAPRQVLPHEREVAGALMTFFLALGLSCGASLSFLFKALL
InterPro domains: - IPR034764: Nucleoside transporter ENT1/ENT2 (family) [18-456]
- IPR036259: MFS transporter superfamily (homologous_superfamily) [16-451]
- IPR002259: Equilibrative nucleoside transporter (family) [7-455]
Interaction partners: - Equilibrative nucleoside transporter 3
- Equilibrative nucleoside transporter 1
- Sodium/nucleoside cotransporter 2
- Sodium/nucleoside cotransporter 1
- Solute carrier family 28 member 3
- Adenosine kinase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity), GO:0015932 (nucleobase-containing compound transmembrane transporter activity), GO:1901505 (carbohydrate derivative transmembrane transporter activity), GO:0005337 (nucleoside transmembrane transporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0065008 (regulation of biological quality), GO:0048856 (anatomical structure development), GO:0042221 (response to chemical), GO:0009719 (response to endogenous stimulus), GO:0051234 (establishment of localization), GO:0051716 (cellular response to stimulus), GO:0050878 (regulation of body fluid levels), GO:1901698 (response to nitrogen compound), GO:0070887 (cellular response to chemical stimulus), GO:0006810 (transport), GO:0048513 (animal organ development), GO:0071495 (cellular response to endogenous stimulus), GO:1901700 (response to oxygen-containing compound), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:0043434 (response to peptide hormone), GO:1901652 (response to peptide), GO:0071417 (cellular response to organonitrogen compound), GO:0032870 (cellular response to hormone stimulus), GO:0071705 (nitrogen compound transport), GO:0010243 (response to organonitrogen compound), GO:0007589 (body fluid secretion), GO:0071310 (cellular response to organic substance), GO:0048732 (gland development), GO:1901699 (cellular response to nitrogen compound), GO:0071702 (organic substance transport), GO:0046903 (secretion), GO:1901701 (cellular response to oxygen-containing compound), GO:0015931 (nucleobase-containing compound transport), GO:0030879 (mammary gland development), GO:1901653 (cellular response to peptide), GO:0007595 (lactation), GO:1901264 (carbohydrate derivative transport), GO:0071375 (cellular response to peptide hormone stimulus), GO:0032868 (response to insulin), GO:0015851 (nucleobase transport), GO:0015858 (nucleoside transport), GO:0032869 (cellular response to insulin stimulus), GO:0006863 (purine nucleobase transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0098793 (presynapse), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0030054 (cell junction), GO:0098794 (postsynapse), GO:0045202 (synapse), GO:0005886 (plasma membrane)