Protein: Twinfilin (Q9VFM9)
Organism: Drosophila melanogaster (Fruit fly)
Function: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin.
Subcellular location: Cytoplasm, cytoskeleton {ECO:0000269|PubMed:11724820}. Cytoplasm, cell cortex {ECO:0000269|PubMed:11724820}.
Sequence: MSHQTGIRANEQLAKVFGKAKNGKFRVIKVSIENEQLSCGATAETKKDWERDYDKLIGPLLEKDVPCYILYRLDAKIPLGYSWLLISWTPDTASIRQKMVYASTKATLKTEFGSAYITEELHATTLDECTLEGYRRHKQDFAAPAPLTSREEELKELRKTEVHTEINTNTRHQTLGGINCPLSEATVAAVQDLVRGKHDYLQFRIDLEEEQIHVSRAAKVELADLPKQVPEDHARYHLFLFRHTHEGDYFESYVFVYSMPGYSCSVRERMMYSSCKAPFLDELAALGVEVVKKLEIDSGSELTEAFLQDELHPKKILHRPAFAKPKGPPNRGAKRLTRPTAED
InterPro domains: - IPR029006: ADF-H/Gelsolin-like domain superfamily (homologous_superfamily) [1-320]
- IPR002108: Actin-depolymerising factor homology domain (domain) [4-312]
- IPR028458: Twinfilin (family) [1-338]
Interaction partners: - Adenylyl cyclase-associated protein
- F-actin-capping protein subunit beta
- Glia maturation factor
- WD repeat-containing protein 82
- Profilin
- Cofilin/actin-depolymerizing factor homolog
- Actin-5C
- F-actin-capping protein subunit alpha
- IP04855p
- Drebrin-like protein
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0008289 (lipid binding), GO:0044877 (protein-containing complex binding), GO:0140096 (catalytic activity, acting on a protein), GO:0043167 (ion binding), GO:0005515 (protein binding), GO:0005543 (phospholipid binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0004672 (protein kinase activity), GO:0043168 (anion binding), GO:0008092 (cytoskeletal protein binding), GO:0035091 (phosphatidylinositol binding), GO:0016301 (kinase activity), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0005546 (phosphatidylinositol-4,5-bisphosphate binding), GO:0004713 (protein tyrosine kinase activity), GO:0003779 (actin binding), GO:0003785 (actin monomer binding), GO:1901981 (phosphatidylinositol phosphate binding), GO:1902936 (phosphatidylinositol bisphosphate binding)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0051641 (cellular localization), GO:0051651 (maintenance of location in cell), GO:0051240 (positive regulation of multicellular organismal process), GO:0071840 (cellular component organization or biogenesis), GO:0051239 (regulation of multicellular organismal process), GO:0033036 (macromolecule localization), GO:0065008 (regulation of biological quality), GO:0051235 (maintenance of location), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0048523 (negative regulation of cellular process), GO:0051129 (negative regulation of cellular component organization), GO:0044057 (regulation of system process), GO:0032507 (maintenance of protein location in cell), GO:0043502 (regulation of muscle adaptation), GO:0032970 (regulation of actin filament-based process), GO:0044087 (regulation of cellular component biogenesis), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0014742 (positive regulation of muscle hypertrophy), GO:0051128 (regulation of cellular component organization), GO:0090066 (regulation of anatomical structure size), GO:0045185 (maintenance of protein location), GO:0032535 (regulation of cellular component size), GO:0043254 (regulation of protein-containing complex assembly), GO:0043244 (regulation of protein-containing complex disassembly), GO:0032956 (regulation of actin cytoskeleton organization), GO:0010613 (positive regulation of cardiac muscle hypertrophy), GO:0090257 (regulation of muscle system process), GO:1902904 (negative regulation of supramolecular fiber organization), GO:0031333 (negative regulation of protein-containing complex assembly), GO:1902903 (regulation of supramolecular fiber organization), GO:0010639 (negative regulation of organelle organization), GO:0043242 (negative regulation of protein-containing complex disassembly), GO:0033043 (regulation of organelle organization), GO:0008104 (protein localization), GO:0032272 (negative regulation of protein polymerization), GO:0030835 (negative regulation of actin filament depolymerization), GO:0051493 (regulation of cytoskeleton organization), GO:0051494 (negative regulation of cytoskeleton organization), GO:1901879 (regulation of protein depolymerization), GO:0032271 (regulation of protein polymerization), GO:0030837 (negative regulation of actin filament polymerization), GO:0030832 (regulation of actin filament length), GO:1901880 (negative regulation of protein depolymerization), GO:0110053 (regulation of actin filament organization), GO:0008064 (regulation of actin polymerization or depolymerization), GO:0051693 (actin filament capping), GO:0030834 (regulation of actin filament depolymerization), GO:0030833 (regulation of actin filament polymerization), GO:0051016 (barbed-end actin filament capping)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0150034 (distal axon), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0044297 (cell body), GO:0030427 (site of polarized growth), GO:0071944 (cell periphery), GO:0030054 (cell junction), GO:0031252 (cell leading edge), GO:0031256 (leading edge membrane), GO:0099080 (supramolecular complex), GO:0032153 (cell division site), GO:0005615 (extracellular space), GO:0036477 (somatodendritic compartment), GO:0005737 (cytoplasm), GO:0043292 (contractile fiber), GO:0120025 (plasma membrane bounded cell projection), GO:0043229 (intracellular organelle), GO:0045202 (synapse), GO:0032154 (cleavage furrow), GO:0005938 (cell cortex), GO:0043228 (non-membrane-bounded organelle), GO:0098590 (plasma membrane region), GO:0097447 (dendritic tree), GO:0030426 (growth cone), GO:0005886 (plasma membrane), GO:0099081 (supramolecular polymer), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0030425 (dendrite), GO:0043005 (neuron projection), GO:0099512 (supramolecular fiber), GO:0030016 (myofibril), GO:0005856 (cytoskeleton), GO:0030424 (axon), GO:0015629 (actin cytoskeleton)