Protein: Cysteine--tRNA ligase 2, cytoplasmic (EC 6.1.1.16) (Cysteinyl-tRNA synthetase) (CysRS) (B3LFA4)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Not known
Subcellular location: Cytoplasm, cytosol {ECO:0000305|PubMed:16251277, ECO:0000305|PubMed:16297076}.
Sequence: MEAEKMELKLYNTMTQQKEVLIPITPGKIGLYVCGITAYDFSHIGHARAAVSFDVLYRYLKHLDYDVTFVRNFTDVDDKIIDRANKNGEDPLDLSNRFCDEYLVDMGALQCLPPTHQPRVSEHMDNIIKMIEKIIEKDCGYVVEGDVFFSVDKSPNYGKLSGQLLEHTRAGERVAVDSRKRNPADFALWKAAKPDEPSWESPWGPGRPGWHIECSAMSVHYLSPKFDIHGGGADLKFPHHENEIAQTCAACEDSGVNYWLHNGHVTINNEKMAKSKHNFKTIREITASYHPLALRHFLMSAQYRSPLSFTASQLESSSEALYYVYQTLQDLDEGLSPYQDALSEDGGKSEQTAEGKDIIKKLKTEFESKMLDDLNTAHILTGAYQDALKFINASLSKLKKMQKKQRMSMLVSLVEIEKAAREVLDVLGLLTTLSYAEILKEMKLKTLIRAEIGEEGISQLIEERITARKNKDFAKSDEIREKLTRKGIALMDIGKETVWRPCFPSQADSST
InterPro domains: - IPR032678: tRNA synthetases class I, catalytic domain (domain) [9-317]
- IPR009080: Aminoacyl-tRNA synthetase, class Ia, anticodon-binding (homologous_superfamily) [352-501]
- IPR015803: Cysteine-tRNA ligase (family) [8-501]
- IPR024909: Cysteinyl-tRNA synthetase/mycothiol ligase (family) [5-507]
- IPR056411: Cysteinyl-tRNA ligase, anticodon binding domain (domain) [455-500]
- IPR014729: Rossmann-like alpha/beta/alpha sandwich fold (homologous_superfamily) [1-310]
Interaction partners: - Leucine--tRNA ligase, cytoplasmic
- Valine--tRNA ligase, chloroplastic/mitochondrial 2
- Valine--tRNA ligase, mitochondrial 1
- Methionine--tRNA ligase, cytoplasmic
- Leucine--tRNA ligase, chloroplastic/mitochondrial
- Histidine--tRNA ligase, cytoplasmic
- Probable elongation factor 1-gamma 2
- Probable threonine--tRNA ligase, cytoplasmic
- Threonine--tRNA ligase, chloroplastic/mitochondrial 2
- Putative glycine--tRNA ligase, cytoplasmic
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:0140098 (catalytic activity, acting on RNA), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0016875 (ligase activity, forming carbon-oxygen bonds), GO:0000166 (nucleotide binding), GO:0043169 (cation binding), GO:0032553 (ribonucleotide binding), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0004812 (aminoacyl-tRNA ligase activity), GO:0140101 (catalytic activity, acting on a tRNA), GO:0032555 (purine ribonucleotide binding), GO:0046872 (metal ion binding), GO:0017076 (purine nucleotide binding), GO:0005524 (ATP binding), GO:0046914 (transition metal ion binding), GO:0032559 (adenyl ribonucleotide binding), GO:0030554 (adenyl nucleotide binding), GO:0008270 (zinc ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0032501 (multicellular organismal process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0071840 (cellular component organization or biogenesis), GO:0007275 (multicellular organism development), GO:0050794 (regulation of cellular process), GO:0009561 (megagametogenesis), GO:0016043 (cellular component organization), GO:0009559 (embryo sac central cell differentiation), GO:0048229 (gametophyte development), GO:0010941 (regulation of cell death), GO:0030154 (cell differentiation), GO:0010197 (polar nucleus fusion), GO:0006996 (organelle organization), GO:0043067 (regulation of programmed cell death), GO:0009553 (embryo sac development), GO:0061024 (membrane organization), GO:0007005 (mitochondrion organization), GO:0007006 (mitochondrial membrane organization), GO:0048284 (organelle fusion), GO:0006997 (nucleus organization), GO:0007007 (inner mitochondrial membrane organization), GO:0000741 (karyogamy), GO:0042407 (cristae formation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)