Protein: Drainin (TBC1 domain family member 12 homolog) (O96904)
Organism: Dictyostelium discoideum (Social amoeba)
Function: May act as a GTPase-activating protein for Rab family protein(s) (By similarity). Required for osmotic regulation by the contractile vacuole in hypo-osmotic environments. Essential for periodic fusion of the contractile vacuole with the plasma membrane and consequent expulsion of water from the cell body.
Subcellular location: Contractile vacuole membrane. Cytoplasm.
Sequence: MICFDNSTAHTDSNNNNNQCDLPENYKELVTTGKVEKLILSPSSSLDRPKTLPPKSQEEVLKHQREYEEIQRKAKKTLEREAKEKEKLDAIRKEKERSLIDARKVWEEDIIPVWEKNSKKRDIKKIKDLSWRGLPPAVRGKIWRLCIGNDLRVTDELFNIFLGHANNAYNKTTSPPLKSSLSSSSKSPNINIDLRQANNSIHHSRSPMNTSTDGEDDVLDLETTNMALILQDIQDTFPSLMIFQKGGPLHSDLIDVLGAYICYRPDIGYVPGMTFLAAMFLLNMEKCDAFLSLSNHINSVCFLPFFRQDQSGIPKYLAAMDSTVEALTPPLHKHFKEIGISAKNYLVDWITTLFSKALPLDVATRIWDLVFIEGEIFIYRTALSILRYFISDLIQATYDECIDLFNKLPQRKISEDKLFEEIQSIVLDQRKFDKLLEK
InterPro domains: - IPR035969: Rab-GAP-TBC domain superfamily (homologous_superfamily) [115-425]
- IPR000195: Rab-GAP-TBC domain (domain) [130-397]
- IPR050302: Rab GTPase-activating TBC domain-containing protein (family) [40-435]
Interaction partners: - BEACH domain-containing protein lvsA
- Ras-related protein Rab-11A
- Ras-related protein Rab-8A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0005488 (binding), GO:0030234 (enzyme regulator activity), GO:0140677 (molecular function activator activity), GO:0005515 (protein binding), GO:0019899 (enzyme binding), GO:0008047 (enzyme activator activity), GO:0060589 (nucleoside-triphosphatase regulator activity), GO:0051020 (GTPase binding), GO:0030695 (GTPase regulator activity), GO:0005096 (GTPase activator activity), GO:0031267 (small GTPase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0023052 (signaling), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0051716 (cellular response to stimulus), GO:0050793 (regulation of developmental process), GO:0051093 (negative regulation of developmental process), GO:0048523 (negative regulation of cellular process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0050794 (regulation of cellular process), GO:0045595 (regulation of cell differentiation), GO:0035556 (intracellular signal transduction), GO:0045596 (negative regulation of cell differentiation), GO:0010453 (regulation of cell fate commitment), GO:0022603 (regulation of anatomical structure morphogenesis), GO:0042659 (regulation of cell fate specification), GO:0007264 (small GTPase mediated signal transduction), GO:0010721 (negative regulation of cell development), GO:0007265 (Ras protein signal transduction)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043227 (membrane-bounded organelle), GO:0055037 (recycling endosome), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0097708 (intracellular vesicle)