Protein: Streptothricin hydrolase (EC 3.5.2.19) (C5NU54)
Organism: Streptomyces noursei (Streptomyces albulus)
Function: Catalyzes the hydrolysis of the amide bond of streptolidine lactam, thereby conferring streptothricin (ST) resistance. Can hydrolyze streptothricin-F and streptothricin-D. However, this strain is believed to be a ST nonproducer, which raises the possibility that its true role may not be its involvement in self-resistance to STs. May catalyze the hydrolysis of naturally occurring cyclic amide compounds that are structurally related to STs.
Sequence: MIRPGRCLWQPCLSGRKRSRPSGPVSRHGMMAAMTAPTAAAIRPVQALLVVDVQAAFVSGWEAVPDADRVLRCTRDLLSRARAAGALVVHLQNDGEPGAVDAPHTPGWELHLPVEPGPRERVVRKTEDDGFADTPLGDLLTDAGVTELAVCGVLSEMCVAATARTALVRGHRVVLPHDAHATYDIPAAPGISDTVPAAMSSRAAEWALGDEVEIVPHAAAVPFAAAPRPAVGPAAAPGLPVSPAAPPPSPVR
InterPro domains: - IPR000868: Isochorismatase-like domain (domain) [47-184]
- IPR036380: Isochorismatase-like superfamily (homologous_superfamily) [28-219]
- IPR050272: Isochorismatase-like hydrolase (family) [45-187]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016810 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds), GO:0016812 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044249 (cellular biosynthetic process), GO:0043094 (cellular metabolic compound salvage), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0006766 (vitamin metabolic process), GO:0006793 (phosphorus metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0043173 (nucleotide salvage), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0006767 (water-soluble vitamin metabolic process), GO:0043101 (purine-containing compound salvage), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0090407 (organophosphate biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:0072524 (pyridine-containing compound metabolic process), GO:0032261 (purine nucleotide salvage), GO:0009117 (nucleotide metabolic process), GO:0019362 (pyridine nucleotide metabolic process), GO:0006163 (purine nucleotide metabolic process), GO:0072522 (purine-containing compound biosynthetic process), GO:1901293 (nucleoside phosphate biosynthetic process), GO:0072525 (pyridine-containing compound biosynthetic process), GO:0046496 (nicotinamide nucleotide metabolic process), GO:0009165 (nucleotide biosynthetic process), GO:0019363 (pyridine nucleotide biosynthetic process), GO:0006164 (purine nucleotide biosynthetic process), GO:0019674 (NAD metabolic process), GO:0009435 (NAD biosynthetic process), GO:0019359 (nicotinamide nucleotide biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)