Protein: Elongation factor G (P0A6M8)
Organism: Escherichia coli (strain K12)
Function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl- tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
Subcellular location: Cytoplasm
Sequence: MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKTRLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEWHQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQAMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLTFFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVTTGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTDEESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQSGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPLAGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETPEENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTMEFLKYDEAPSNVAQAVIEARGK
InterPro domains: - IPR000795: Translational (tr)-type GTP-binding domain (domain) [8-290]
- IPR005517: Translation elongation factor EFG/EF2, domain IV (domain) [486-609]
- IPR047872: Elongation factor G, domain IV (domain) [490-609]
- IPR009000: Translation protein, beta-barrel domain superfamily (homologous_superfamily) [271-411]
- IPR020568: Ribosomal protein uS5 domain 2-type superfamily (homologous_superfamily) [486-610]
- IPR000640: Elongation factor EFG, domain V-like (domain) [611-698]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [1-298]
- IPR014721: Small ribosomal subunit protein uS5 domain 2-type fold, subgroup (homologous_superfamily) [492-699]
- IPR035647: EF-G domain III/V-like (homologous_superfamily) [411-700]
- IPR005225: Small GTP-binding domain (domain) [10-187]
- IPR041095: Elongation Factor G, domain II (domain) [411-485]
- IPR031157: Tr-type G domain, conserved site (conserved_site) [51-66]
- IPR009022: Elongation factor G, domain III (domain) [412-487]
- IPR004161: Translation elongation factor EFTu-like, domain 2 (domain) [331-398]
- IPR004540: Translation elongation factor EFG/EF2 (family) [1-703]
- IPR035649: EFG, domain V (domain) [614-691]
Interaction partners: - Small ribosomal subunit protein uS2
- Small ribosomal subunit protein uS2
- Small ribosomal subunit protein uS2
- Large ribosomal subunit protein uL13
- Small ribosomal subunit protein uS13
- Large ribosomal subunit protein uL18
- Small ribosomal subunit protein uS10
- Small ribosomal subunit protein uS7
- Large ribosomal subunit protein uL24
- Large ribosomal subunit protein bL9
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0032553 (ribonucleotide binding), GO:0097216 (guanosine tetraphosphate binding), GO:0032555 (purine ribonucleotide binding), GO:0017076 (purine nucleotide binding), GO:0019001 (guanyl nucleotide binding), GO:0032561 (guanyl ribonucleotide binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0051716 (cellular response to stimulus), GO:0009628 (response to abiotic stimulus), GO:0006950 (response to stress), GO:0009266 (response to temperature stimulus), GO:0009409 (response to cold), GO:0033554 (cellular response to stress), GO:0070417 (cellular response to cold)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0005737 (cytoplasm)