Protein: Mitosis inhibitor protein kinase SWE1 (Q5AP97)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Protein kinase that acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylating and inhibiting the mitosis-promoting cyclin B-bound CDC28 at 'Tyr-18'. SWE1-mediated inhibition of CDC28 acts in a cell size or morphogenesis checkpoint to delay mitosis in response to defects in growth, actin organization or bud formation. Plays an important role in filamentous growth
Subcellular location: Bud neck. Nucleus
Sequence: MDSNPCQDVSGDTSSTPMANNNPTNDSTISSQNHSKIGLRKHQQQHYHQHSHSQMHSHSQQSPYINQLEYFTNNQFSRSFNSLILEDANDANTNNSSTTTLNKKTINKSPPFNIKQDLLNDSIDTFLDNSNTETIEDGDVTTTDDDHDFDDEDIEDPEAVQYTPTLNILKSKKVDSFDIISSKHRKSNSQITYNSHVRKPSEEDTSSSMATIRLSNNSQSSIKRSSKYLNLSIDSNLKTVDGGKIPDEIDDISLNEIDVAVAPNDFSSPLSARKPDIFAAITAANGNSNNQFKRPHKLVSQSPSPSSKNKFRISSSTTSSPQSNLHSPSKLGSKGFKMFKNANRDAIMSSSRVMTPEKPKMVSKIFGKSAKIRRAYTPTHTSTPMAVSSLNPPSSSTSNSTTAAITSTSPPANEHYDIDNDFDSPSKNRKSSNISASSIIIYQDENHIKSNHARKSSNPIPYPPTEPLPTNISASVAETGKGSTTTKSNLSKGCPLFDDKENKASYQFVKPLQTAFNSSGLVKKNSISGSSDRKLPPETPIKRNPLMILNTNKVVPPYSSGFAEGKDVMGDQHDIYSHIPCQNQRFPGSVNPNTTTNNNNTQQHHDSDLSIEVGRNNSYDASSSTINNTSYIKIFPSSELKKEQVLQRPQEDLELVFNSDIELDDNIIPETPTKKSLLPNQHHQHHLPLYTQSKSPLLKFDTEKDGRRNLSIVLDKSNATKREISEPPSTPINMSFAKNSFKKPMNNAERGDDPDSIIAQRIDIMPSLDEADSVSVYPSKIDEHLIEKFGMKNIKYIGSGAFSIAFECLFNNEKFAIKRTKKPLIGKLEKQTIKREIEALRVLTSIKEDEATNMQEQEEGKEYLVYFIEAWDFNNYYYIMTEFCEGGTLFDFLEENKHYKIDEFRIWKILIEILNGLKFIHSKNYLHLDLKPANIFITFEGSLKIGDFGLATKLPILEKDFDLEGDRNYIAPELINDKIYTPFADIFSLGLIILEIAANIILPDNGTPWRKLRSGDLSDAGRLSSDNISMFLQHNPNTNSNISGSGSRSGSGSTGGNGSAGDGSTNSTNFSYNSLSGNSLTLNPPVKAVHGTSDTNNTLASELSKNIEGLIPSWAPDFLVHGDSMNLDKLVNKMLRPNPFDRPSACNILEMPECLIVENRRKCGATIFEGEFGSPPDE
InterPro domains: - IPR008271: Serine/threonine-protein kinase, active site (active_site) [925-937]
- IPR050339: Cell Cycle and Stress Response Kinase (family) [239-1151]
- IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [792-1152]
- IPR017441: Protein kinase, ATP binding site (binding_site) [797-827]
- IPR000719: Protein kinase domain (domain) [791-1155]
Interaction partners: - Serine/threonine-protein kinase
- G2/mitotic-specific cyclin CLB2
- Cyclin-dependent kinase 1
- Serine/threonine-protein kinase RAD53
- Tyrosine-protein phosphatase CDC14
- Protein phosphatase PP2A regulatory subunit B
- Serine/threonine-protein kinase HSL1
- Serine/threonine-protein kinase GIN4
- M-phase inducer phosphatase
- Kleisin alpha
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004713 (protein tyrosine kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0023052 (signaling), GO:0000003 (reproduction), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0022414 (reproductive process), GO:0040007 (growth), GO:0048519 (negative regulation of biological process), GO:2000241 (regulation of reproductive process), GO:0051321 (meiotic cell cycle), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:0071840 (cellular component organization or biogenesis), GO:0032504 (multicellular organism reproduction), GO:2000242 (negative regulation of reproductive process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0048523 (negative regulation of cellular process), GO:0007165 (signal transduction), GO:0007154 (cell communication), GO:0051726 (regulation of cell cycle), GO:1901993 (regulation of meiotic cell cycle phase transition), GO:0051129 (negative regulation of cellular component organization), GO:1901993 (regulation of meiotic cell cycle phase transition), GO:0035556 (intracellular signal transduction), GO:0045786 (negative regulation of cell cycle), GO:0051447 (negative regulation of meiotic cell cycle), GO:0010948 (negative regulation of cell cycle process), GO:0051726 (regulation of cell cycle), GO:0051445 (regulation of meiotic cell cycle), GO:0016043 (cellular component organization), GO:0045786 (negative regulation of cell cycle), GO:0051447 (negative regulation of meiotic cell cycle), GO:0051128 (regulation of cellular component organization), GO:0010564 (regulation of cell cycle process), GO:0007346 (regulation of mitotic cell cycle), GO:0007088 (regulation of mitotic nuclear division), GO:0000075 (cell cycle checkpoint signaling), GO:0045839 (negative regulation of mitotic nuclear division), GO:1905818 (regulation of chromosome separation), GO:0007093 (mitotic cell cycle checkpoint signaling), GO:0010639 (negative regulation of organelle organization), GO:0051983 (regulation of chromosome segregation), GO:1901987 (regulation of cell cycle phase transition), GO:0045930 (negative regulation of mitotic cell cycle), GO:1905819 (negative regulation of chromosome separation), GO:0033043 (regulation of organelle organization), GO:0051985 (negative regulation of chromosome segregation), GO:0006996 (organelle organization), GO:1901988 (negative regulation of cell cycle phase transition), GO:0051783 (regulation of nuclear division), GO:0051784 (negative regulation of nuclear division), GO:0010965 (regulation of mitotic sister chromatid separation), GO:0033045 (regulation of sister chromatid segregation), GO:1901991 (negative regulation of mitotic cell cycle phase transition), GO:2000816 (negative regulation of mitotic sister chromatid separation), GO:0033046 (negative regulation of sister chromatid segregation), GO:1902099 (regulation of metaphase/anaphase transition of cell cycle), GO:0033048 (negative regulation of mitotic sister chromatid segregation), GO:0031577 (spindle checkpoint signaling), GO:0045841 (negative regulation of mitotic metaphase/anaphase transition), GO:0033047 (regulation of mitotic sister chromatid segregation), GO:1901990 (regulation of mitotic cell cycle phase transition), GO:1902100 (negative regulation of metaphase/anaphase transition of cell cycle), GO:2001251 (negative regulation of chromosome organization), GO:0033044 (regulation of chromosome organization), GO:0071174 (mitotic spindle checkpoint signaling), GO:0007094 (mitotic spindle assembly checkpoint signaling), GO:0071173 (spindle assembly checkpoint signaling), GO:0030071 (regulation of mitotic metaphase/anaphase transition)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005815 (microtubule organizing center), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:1902494 (catalytic complex), GO:0030427 (site of polarized growth), GO:0005933 (cellular bud), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:1990234 (transferase complex), GO:0043229 (intracellular organelle), GO:0005935 (cellular bud neck), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0005813 (centrosome), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0061695 (transferase complex, transferring phosphorus-containing groups), GO:0005856 (cytoskeleton), GO:0031981 (nuclear lumen), GO:0005819 (spindle), GO:0005634 (nucleus), GO:1902911 (protein kinase complex), GO:1902554 (serine/threonine protein kinase complex), GO:0015630 (microtubule cytoskeleton)