Protein: ATP-dependent zinc metalloprotease FtsH (EC 3.4.24.-) (Cell division protease FtsH) (P0AAI3)
Organism: Escherichia coli (strain K12)
Function: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins. Degrades a few membrane proteins that have not been assembled into complexes such as SecY, F(0) ATPase subunit a and YccA, and also cytoplasmic proteins sigma-32, LpxC, KdtA and phage lambda cII protein among others. Degrades membrane proteins in a processive manner starting at either the N- or C-terminus; recognition requires a cytoplasmic tail of about 20 residues with no apparent sequence requirements. It presumably dislocates membrane-spanning and periplasmic segments of the protein into the cytoplasm to degrade them, this probably requires ATP. Degrades C-terminal-tagged cytoplasmic proteins which are tagged with an 11-amino-acid nonpolar destabilizing tail via a mechanism involving the 10SA (SsrA) stable RNA.
Subcellular location: Cell inner membrane {ECO:0000255|HAMAP-Rule:MF_01458, ECO:0000269|PubMed:15919996, ECO:0000269|PubMed:8444797, ECO:0000269|PubMed:8947034}; Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01458, ECO:0000269|PubMed:15919996, ECO:0000269|PubMed:8444797, ECO:0000269|PubMed:8947034}.
Sequence: MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNNDQVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASIFISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEAKEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVHMRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKIMMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLPEGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWGFSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTDNMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRPVDEPRTPNPGNTMSEQLGDK
InterPro domains: - IPR000642: Peptidase M41 (domain) [401-591]
- IPR003593: AAA+ ATPase domain (domain) [184-323]
- IPR041569: AAA ATPase, AAA+ lid domain (domain) [343-387]
- IPR003959: ATPase, AAA-type, core (domain) [188-320]
- IPR037219: Peptidase M41-like (homologous_superfamily) [396-602]
- IPR011546: Peptidase M41, FtsH extracellular (domain) [5-93]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [141-391]
- IPR005936: ATP-dependent zinc metalloprotease, FtsH (family) [3-612]
- IPR003960: ATPase, AAA-type, conserved site (conserved_site) [291-309]
Interaction partners: - Modulator of FtsH protease HflC
- Modulator of FtsH protease HflK
- ATP-dependent Clp protease ATP-binding subunit ClpX
- Ribosomal RNA large subunit methyltransferase E
- Chaperone protein DnaK
- Lon protease
- ATP-dependent Clp protease proteolytic subunit
- ATP-dependent protease ATPase subunit HslU
- Chaperone protein DnaJ
- RNA polymerase sigma factor RpoH
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0008233 (peptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:1901575 (organic substance catabolic process), GO:0043170 (macromolecule metabolic process), GO:0006091 (generation of precursor metabolites and energy), GO:0009057 (macromolecule catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0030163 (protein catabolic process), GO:0015980 (energy derivation by oxidation of organic compounds), GO:0030163 (protein catabolic process), GO:0045333 (cellular respiration)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:1902494 (catalytic complex), GO:0098796 (membrane protein complex), GO:0034357 (photosynthetic membrane), GO:1905368 (peptidase complex), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0042651 (thylakoid membrane), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0009579 (thylakoid)