Protein: Ubiquitin-like modifier-activating enzyme 6 (Ubiquitin-activating enzyme 6) (EC 6.2.1.45) (Ubiquitin-activating enzyme E1-like protein 2) (E1-L2) (Q8C7R4)
Organism: Mus musculus (Mouse)
Function: Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34 (By similarity). Essential for embryonic development. Required for UBD/FAT10 conjugation.
Sequence: MERSEPLAVLSCEEASCSSWGACGASKNLPTMTTESLEIDDGLYSRQRYVLGDTAMQKMAKSCVFLSGMGGLGVEIAKNLVLAGIKALTIHDTKKCQAWDLGTNFFLCEDDVVNERNRAEAVLHRIAELNPYVQVSSSSAPLDETTDLSFLEKYQCVVLTEIKLTLQKKINNFCHSHCPPIKFISADVHGIWSRLFCDFGDEFEVSDTTGEEPKEIFISNITQANPGIVTCLESHPHKLETGQFLTFREIHGMTGLNGSVQQITVISPFSFSIGDTTKLDPYLHGGIAVQVKTPKTFCFEPLESQIKHPRCLIADFSKPEAPLEIHLAMLALDQFQENYNRKPNIRCQQDSDELLKLTVSINETLEEKPEVNADIVHWLSWTAQGFLPPLAAAVGGVASQEVLKAVTGKFSPLCQWLYLEAADTVESLGNPGHEEFLPRGDRYDAIRACIGNTLCQKLQNLNIFLVGCGAIGCEMLKNFALLGVGTGREKGMVTVTDPDLIEKSNLNRQFLFRPHHIQKPKSYTAAEATLKINPQLKIDAHLNKVCPATESIYSDEFYTKQDIIITALDNVEARRYVDSRCLANLRPLLDSGTMGTKGHTEIIVPQLTESYNSHRDPPEEEIPFCTLKSFPAAIEHTIQWARDKFESSFSHKPSLFNKFWQAYPSAEDVLQKIQNGQSLEGCFQVIKLLSRRPRIWSQCVELARLKFEKYFNHKALQLLHCFPLETRLKDGSLFWQSPKRPPSPIKFDLNEPLHLSFLQSAAKLYATVYCIPFSEKDLSVNSLMDILSEVKIEEFKPSNKVVQTDETARKPDHVPVSSEDERNAVFQLEEALSSNKATKSDLQMTVLSFEKDDDRNGHIDFITAASNLRAKMYSIEPADRFKTKRIAGKIIPAIATSTAAVSGLVALEMIKVAGGYPFDAYKNCFLNLAIPIIVFTETSEVRKTEIRNGISFTIWDRWTVHGKEDFTLSDFINAVKENYGIEPTMVVQGVKMLYVPVMPGHAKRLKLTMHKLVKPSTEKKYVDLTVSFAPDADGDEDLPGPPVRYYFSHDTNE
InterPro domains: - IPR018075: Ubiquitin-activating enzyme E1 (family) [38-1048]
- IPR032418: Ubiquitin-activating enzyme E1, FCCH domain (domain) [225-292]
- IPR019572: Ubiquitin-activating enzyme, SCCH domain (domain) [631-884]
- IPR018965: Ubiquitin-activating enzyme E1, C-terminal (domain) [921-1043]
- IPR042302: Ubiquitin-activating enzyme E1, FCCH domain superfamily (homologous_superfamily) [212-291]
- IPR032420: Ubiquitin-activating enzyme E1, four-helix bundle (domain) [293-362]
- IPR042449: Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1 (homologous_superfamily) [35-158]
- IPR045886: ThiF/MoeB/HesA family (family) [382-1013]
- IPR000594: THIF-type NAD/FAD binding fold (domain) [44-931]
- IPR038252: Ubiquitin-activating enzyme E1, C-terminal domain superfamily (homologous_superfamily) [957-1047]
- IPR042063: Ubiquitin-activating enzyme E1, SCCH domain (homologous_superfamily) [616-891]
- IPR000011: Ubiquitin/SUMO-activating enzyme E1-like (family) [65-718]
- IPR035985: Ubiquitin-activating enzyme-like (homologous_superfamily) [38-937]
Interaction partners: - Ubiquitin D
- Ubiquitin-conjugating enzyme E2 Z
- Ubiquitin-ribosomal protein eS31 fusion protein
- Ubiquitin-conjugating enzyme E2 N
- Ubiquitin-conjugating enzyme E2 K
- Polyubiquitin-B
- Ubiquitin-conjugating enzyme E2 G1
- Ubiquitin-conjugating enzyme E2 R2
- Ubiquitin-conjugating enzyme E2 A
- Ubiquitin-conjugating enzyme E2 R1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0016874 (ligase activity), GO:0008641 (ubiquitin-like modifier activating enzyme activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016877 (ligase activity, forming carbon-sulfur bonds)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044238 (primary metabolic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0006807 (nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0044248 (cellular catabolic process), GO:0036211 (protein modification process), GO:0009057 (macromolecule catabolic process), GO:0043412 (macromolecule modification), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0044265 (cellular macromolecule catabolic process), GO:0030163 (protein catabolic process), GO:0070647 (protein modification by small protein conjugation or removal), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0051603 (proteolysis involved in protein catabolic process), GO:0032446 (protein modification by small protein conjugation), GO:0019941 (modification-dependent protein catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0005737 (cytoplasm)