Protein: Secreted effector protein SseJ (EC 3.-.-.-) (Salmonella-translocated effector J) (STE J) (Q9FD10)
Organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Function: Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is required for endosomal tubulation and negatively regulates the formation of Salmonella-induced filaments (Sifs) in epithelial cells. Has both deacylase and esterification activities in vitro, but esterification is probably the dominant activity in host cells. Significantly contributes to cholesterol esterification, which reduces cellular cholesterol in cells and abrogates the ability of SifA to associate with cholesterol and LAMP-1 vesicles.
Subcellular location: Secreted. Host cytoplasm. Note=Secreted via type III secretion system 2 (SPI-2 T3SS), and delivered into the host cytoplasm. Localizes to the Salmonella-containing vacuole (SCV) at early time points following invasion and subsequently traffics away from the SCV along Salmonella-induced filaments (Sifs) in epithelial cells.
Sequence: MPLSVGQGYFTSSISSEKFNAIKESARLPELSLWEKIKAYFFTTHHAEALECIFNLYHHQELNLTPVQVRGAYIKLRALASQGCKEQFIIESQEHADKLIIKDDNGENILSIEVECHPEAFGLAKEINKSHPKPKNISLGDITRLVFFGDSLSDSLGRMFEKTHHILPSYGQYFGGRFTNGFTWTEFLSSPHFLGKEMLNFAEGGSTSASYSCFNCIGDFVSNTDRQVASYTPSHQDLAIFLLGANDYMTLHKDNVIMVVEQQIDDIEKIISGGVNNVLVMGIPDLSLTPYGKHSDEKRKLKDESIAHNALLKTNVEELKEKYPQHKICYYETADAFKVIMEAASNIGYDTENPYTHHGYVHVPGAKDPQLDICPQYVFNDLVHPTQEVHHCFAIMLESFIAHHYSTE
InterPro domains: - IPR001087: GDSL lipase/esterase (family) [145-394]
- IPR010637: Sif (family) [1-108]
- IPR036514: SGNH hydrolase superfamily (homologous_superfamily) [137-405]
Interaction partners: - Secreted effector protein SifB
- Deubiquitinase SseL
- Secreted effector protein SifA
- Secreted effector kinase SteC
- Secreted effector protein PipB
- Secreted effector protein PipB2
- Secreted effector protein sopD2
- Secretion system effector
- Secretion system effector
- Secreted effector protein SptP
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0044403 (biological process involved in symbiotic interaction), GO:0035821 (modulation of process of another organism), GO:0051701 (biological process involved in interaction with host), GO:0044003 (modulation by symbiont of host process), GO:0052111 (modification by symbiont of host structure), GO:0052043 (modification by symbiont of host cellular component)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0018995 (host cellular component), GO:0033643 (host cell part), GO:0043657 (host cell), GO:0033644 (host cell membrane), GO:0033646 (host intracellular part), GO:0043656 (host intracellular region), GO:0033655 (host cell cytoplasm part), GO:0033647 (host intracellular organelle), GO:0020002 (host cell plasma membrane), GO:0030430 (host cell cytoplasm), GO:0033648 (host intracellular membrane-bounded organelle), GO:0044165 (host cell endoplasmic reticulum)