Protein: Alpha-galactosidase 3 (AtAGAL3) (EC 3.2.1.22) (Alpha-D-galactoside galactohydrolase 3) (Melibiase) (Q8VXZ7)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: May regulate leaf (and possibly other organ) development by functioning in cell wall loosening and cell wall expansion.
Subcellular location: Secreted, cell wall {ECO:0000250|UniProtKB:Q8RX86}. Secreted, extracellular space, apoplast {ECO:0000250|UniProtKB:Q8RX86}. Vacuole {ECO:0000305|PubMed:12773619}.
Sequence: MVIMKKMKDSVLFLVVGLFSLSVLVSQSIAGRVKAPLLQSNTGGLVFSKSFNSIYDTSMYGRLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNIDDCWSNLLRDSEGQLVPHPETFPSGIKLLADYVHSKGLKLGIYSDAGVFTCEVHPGSLFHEVDDADIFASWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPALWAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAHDCHMYVLTPQTVSHSDV
InterPro domains: - IPR000111: Glycoside hydrolase family 27/36, conserved site (conserved_site) [108-124]
- IPR017853: Glycoside hydrolase superfamily (homologous_superfamily) [63-336]
- IPR002241: Glycoside hydrolase, family 27 (family) [19-429]
- IPR013785: Aldolase-type TIM barrel (homologous_superfamily) [65-337]
- IPR041233: Alpha galactosidase, C-terminal domain (domain) [352-427]
- IPR013780: Glycosyl hydrolase, all-beta (homologous_superfamily) [338-430]
Interaction partners: - Probable galactinol--sucrose galactosyltransferase 5
- Probable galactinol--sucrose galactosyltransferase 2
- Probable galactinol--sucrose galactosyltransferase 1
- Probable galactinol--sucrose galactosyltransferase 6
- alpha-D-xyloside xylohydrolase
- Probable galactinol--sucrose galactosyltransferase 4
- beta-galactosidase
- Beta-hexosaminidase 1
- Beta-hexosaminidase 3
- Aldose 1-epimerase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016798 (hydrolase activity, acting on glycosyl bonds), GO:0004553 (hydrolase activity, hydrolyzing O-glycosyl compounds), GO:0015925 (galactosidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0032501 (multicellular organismal process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009605 (response to external stimulus), GO:0050793 (regulation of developmental process), GO:2000243 (positive regulation of reproductive process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0051239 (regulation of multicellular organismal process), GO:0009653 (anatomical structure morphogenesis), GO:2000241 (regulation of reproductive process), GO:0051094 (positive regulation of developmental process), GO:0009607 (response to biotic stimulus), GO:0051707 (response to other organism), GO:0051240 (positive regulation of multicellular organismal process), GO:0010016 (shoot system morphogenesis), GO:0099402 (plant organ development), GO:0048582 (positive regulation of post-embryonic development), GO:0048580 (regulation of post-embryonic development), GO:0009911 (positive regulation of flower development), GO:0048831 (regulation of shoot system development), GO:2000026 (regulation of multicellular organismal development), GO:0043207 (response to external biotic stimulus), GO:1905392 (plant organ morphogenesis), GO:0009909 (regulation of flower development), GO:0009620 (response to fungus), GO:0048731 (system development), GO:0048367 (shoot system development), GO:0048827 (phyllome development), GO:0009965 (leaf morphogenesis), GO:0048366 (leaf development)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0071944 (cell periphery), GO:0005737 (cytoplasm), GO:0030312 (external encapsulating structure), GO:0005576 (extracellular region), GO:0048046 (apoplast), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0005618 (cell wall), GO:0099503 (secretory vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0009505 (plant-type cell wall), GO:0097708 (intracellular vesicle)