Protein: Katanin p60 ATPase-containing subunit A1 (Katanin p60 subunit A1) (EC 5.6.1.1) (p60 katanin) (O75449)
Organism: Homo sapiens (Human)
Function: Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:10751153, ECO:0000269|PubMed:26929214, ECO:0000269|PubMed:9658175}. Midbody {ECO:0000269|PubMed:19261606}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000255|HAMAP-Rule:MF_03023}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:10751153, ECO:0000269|PubMed:26929214, ECO:0000269|PubMed:9658175}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:26929214}. Note=Predominantly cytoplasmic (PubMed:9658175). Localized diffusely in the cytoplasm during the interphase (PubMed:10751153). During metaphase is localized throughout the cell and more widely dispersed than the microtubules. In anaphase and telophase is localized at the midbody region (PubMed:19261606). Also localized to the interphase centrosome and the mitotic spindle poles (By similarity). Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1 (PubMed:10751153). Localizes within the cytoplasm, partially overlapping with microtubules, in interphase and to the mitotic spindle and spindle poles during mitosis (PubMed:26929214). {ECO:0000255|HAMAP-Rule:MF_03023, ECO:0000269|PubMed:10751153, ECO:0000269|PubMed:19261606, ECO:0000269|PubMed:26929214, ECO:0000269|PubMed:9658175}.
Sequence: MSLLMISENVKLAREYALLGNYDSAMVYYQGVLDQMNKYLYSVKDTYLQQKWQQVWQEINVEAKHVKDIMKTLESFKLDSTPLKAAQHDLPASEGEVWSMPVPVERRPSPGPRKRQSSQYSDPKSHGNRPSTTVRVHRSSAQNVHNDRGKAVRCREKKEQNKGREEKNKSPAAVTEPETNKFDSTGYDKDLVEALERDIISQNPNVRWDDIADLVEAKKLLKEAVVLPMWMPEFFKGIRRPWKGVLMVGPPGTGKTLLAKAVATECKTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYSPATIFIDEIDSICSRRGTSEEHEASRRVKAELLVQMDGVGGTSENDDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPLPSAKGREELLRISLRELELADDVDLASIAENMEGYSGADITNVCRDASLMAMRRRIEGLTPEEIRNLSKEEMHMPTTMEDFEMALKKVSKSVSAADIERYEKWIFEFGSC
InterPro domains: - IPR003593: AAA+ ATPase domain (domain) [241-383]
- IPR028596: Katanin p60 subunit A1 (family) [4-491]
- IPR041569: AAA ATPase, AAA+ lid domain (domain) [403-445]
- IPR003959: ATPase, AAA-type, core (domain) [245-381]
- IPR048612: Katanin p60 ATPase-containing subunit A1, AAA domain (domain) [210-379]
- IPR015415: Spastin/Vps4, C-terminal (domain) [451-489]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [203-485]
- IPR048611: Katanin p60 ATPase-containing subunit A1, MIT domain (domain) [5-72]
- IPR050304: Microtubule-severing AAA ATPase (family) [21-490]
- IPR003960: ATPase, AAA-type, conserved site (conserved_site) [353-372]
Interaction partners: - Katanin p80 WD40 repeat-containing subunit B1
- KATNB1-like protein 1
- Leucine zipper putative tumor suppressor 2
- Nuclear distribution protein nudE-like 1
- Abnormal spindle-like microcephaly-associated protein
- Glycerol-3-phosphate dehydrogenase, mitochondrial
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0140776 (protein-containing complex destabilizing activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0042802 (identical protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0007017 (microtubule-based process), GO:0071840 (cellular component organization or biogenesis), GO:0016043 (cellular component organization), GO:0000226 (microtubule cytoskeleton organization), GO:0006996 (organelle organization), GO:0051013 (microtubule severing), GO:0007010 (cytoskeleton organization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005815 (microtubule organizing center), GO:0005875 (microtubule associated complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0000922 (spindle pole), GO:0099080 (supramolecular complex), GO:0099081 (supramolecular polymer), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0099512 (supramolecular fiber), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0099513 (polymeric cytoskeletal fiber), GO:0005819 (spindle), GO:0005874 (microtubule), GO:0015630 (microtubule cytoskeleton)