Protein: 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase (EC 2.3.1.245) (P76143)
Organism: Escherichia coli (strain K12)
Function: Involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle. Catalyzes the transfer of an acetyl moiety from 3-hydroxy-5-phosphonooxypentane-2,4-dione to CoA to form glycerone phosphate and acetyl-CoA.
Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02052}.
Sequence: MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNRPVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ
InterPro domains: - IPR033673: 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase (family) [1-291]
- IPR050456: DeoC/FbaB aldolase (family) [33-287]
- IPR013785: Aldolase-type TIM barrel (homologous_superfamily) [1-291]
- IPR002915: DeoC/FbaB/LacD aldolase (family) [51-259]
- IPR041720: Aldolase FbaB-like, archaeal-type (family) [31-289]
Interaction partners: - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase (Autoinducer 2-degrading protein LsrG) (AI-2-degrading protein LsrG) (Phospho-(S)-4,5-dihydroxy-2,3-pentanedione isomerase) (Phospho-AI-2 isomerase)
- Autoinducer 2-binding protein LsrB
- Autoinducer 2 import system permease protein LsrD
- Autoinducer-2 kinase
- Transcriptional regulator LsrR
- Autoinducer 2 import system permease protein LsrC
- Autoinducer 2 import ATP-binding protein LsrA
- Trans-aconitate 2-methyltransferase
- S-ribosylhomocysteine lyase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0005515 (protein binding), GO:0016829 (lyase activity), GO:0016830 (carbon-carbon lyase activity), GO:0042802 (identical protein binding), GO:0016832 (aldehyde-lyase activity), GO:0004332 (fructose-bisphosphate aldolase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0006082 (organic acid metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044249 (cellular biosynthetic process), GO:0005996 (monosaccharide metabolic process), GO:0005975 (carbohydrate metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0006793 (phosphorus metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0019318 (hexose metabolic process), GO:0046364 (monosaccharide biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0016053 (organic acid biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0090407 (organophosphate biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0019752 (carboxylic acid metabolic process), GO:0009117 (nucleotide metabolic process), GO:0046390 (ribose phosphate biosynthetic process), GO:0006163 (purine nucleotide metabolic process), GO:0072522 (purine-containing compound biosynthetic process), GO:1901293 (nucleoside phosphate biosynthetic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0009165 (nucleotide biosynthetic process), GO:0032787 (monocarboxylic acid metabolic process), GO:0006164 (purine nucleotide biosynthetic process), GO:0072329 (monocarboxylic acid catabolic process), GO:0009152 (purine ribonucleotide biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)