Protein: Fatty acid synthase (E7F5V3)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain
Sequence: MEDIVIAGISGRLPESNNLEEFWQNLFNGVDMVTEDDRRWKPGLYGLPRRNGKLKEIDRFDAAFFGVHPKQAHTMDPQLRLMLEISYEAIVDGGINPVSMRGSKTGVYIGVSGSEAGEAFSKDPEELLGYSMTGCQRAMFANRLSYFFDFNGPSTAIDTACSSSLLALENAFNAIRHGKCDAALIGGVNLLLKPNTSVQFMKLGMLSPEGTCKSFDASGNGYCRSEAAVAVLLTKKSMAKRIYATVLNAGNNTDGYKEQGVTFPSGEMQQRLVRSLYQEANISPEQVEYIEAHGTGTKVGDPQEVNGIVSVFCQSQRDPLLIGSTKSNMGHPEPASGLAALAKVVLSLEHGVWAPNVHFNEPNPDIPALTDGRLCVVSKPTPVRGGIVGINSFGFGGSNVHVILSPHVSDSSDKTPAPSVPRLLQASGRTEEAVTALFSNAQQHQENSSYLSLLNDVSGVPTAGMPYRGYTLIGAQGELTEVQQTQPTPRPLWYICSGMGTQWAGMGQSLMQLSEFRESIKRSDIALKDTGLCVSRLLMEADESTFEDTVHAFVGLAAIQVAQIDMLRKMGLEPNGIVGHSVGELACGYADGSLSHSEAILAAYWRGRCIKEANLPPGGMAAVGLTWEECKAQCPQGVVPACHNAEDTVTISGPQDSVSKFVAQLKESGVFAKEVRSAGVAFHSYYMASIAPALLSALQKVIKSPRPRTARWISTSIPQSDWESPLALYSSAEYHVNNLVSPVLFQEGLNHVPDNAVVVEIAPHALLQAILKRSLKPTCSILPLMKRGHANNLEFFLSHVGKVYMNGINVDSNKLYPAVKYPVPRGTPLISPYIQWDHSQSWDVPKVEDFPAGSGGSTSATVYNIDMNPESPDYYMIGHCIDGRVLYPATGYLVLAWRTLMRSLGTVMDHTPVTFEDVTIHRATILPKTGSVQLEVRLMPATNRFEVSENGNLAVSGKVSVLEDSGLDAFHAELNKPITADNEDPKLRLKSGDIYKELRLRGYDYGKTFQGILESNNAGDSGKLHWTGNWVTFLDTMLQMIVVGLPGRSLRLPTRIRSVCVDPKLHEERVNDYEGDQKAVNVFVNRCLDNITAGGVQICGLHATAAPRRQQQQTPPTLEEFVFVPYEDADCLRTNEKLADQLRHCKGLVQRLQRKLAKQGVKISIPGLEGASEGQLIEAEAEKGLLRLLSVLCGLELNGNLRSELEQTVQKERDCLLQDPLLNGLLDSQALRHCLDTALENSTPGKFKVLEALAADGRVFSQAVSLLNIQPMLRLDYTASDISADQLSAQQSSLEEQGISTAQWDPLQGPVTGGLDGADLVVCNCAVGSATNPALLIENLTSAAREGGFILLHTLLRGDTLGETVAFLTSQNNRKGLLTQTEWEELFQKASLNVVMLRKSYYGSALFLCRRSQQSSQKQPIHIFVDPTDYKWVETLKSTLAESSDIPVWLIATKGHNGVVGMVNCLRQEPGGNRIRCTFVSNLSKGAAVPSLLPNEKVMKALLKKDLVMNVHRDGLWGVFRHQLLTQDLSEELTEQAYVNVLTRGDLSSLRWIASPLRHFVASSPNVQLCRVYYSSLNFRDIMLATGKLPPDAIPGDVALQQCMLGMEFSGRDPSGRRVMGLLPAKGLATCVDADKRFLWDVPSSWTLEQAASVPVVYATAYYSLVVRGRLRPGESVLIHSGSGGVGQAAIAIALSMRCRVFTTVGSKEKKQYLQERFPQLTAESFANSRDSSFEQHVMLNTQGKGVDLVLNSLAEEKLQASLRCLARHGRFLEIGKYDLSNNTPLGMALFLKNVAFHGILLDALFEEGNREWEEVSDLLKKGISSGVVQPLRTTVFERNQVEEAFRYMAQGKHIGKVLLQVRSEESSSSGPAVSALSIPAICRTFCPASLSYIITGGLGGFGLELAQWLTERGARKLVLTSRSGIRNGYQAKRVREWQAMGIQVLVSTSDVSTLDGTERLITEACRLGPVGGIFHLAMVLKDGMLENLTPQEFIEVNRPKYDGTINLDSVTRQKCPQLQQFVVFSSVSCGRGNAGQSNYGFANSTMERICEQRRQENLPGLAVQWGAIGDVGVVLETMGGNDAVIGGTLPQRMSSCLEVLDRFLCQQRPVMSSFVLAEKVVVTKGEGSGQKDLVEAVAHILGVRDVNSLNADASLADLGLDSLMGVEVRQTLERDYDIVMAMREIRQLTINKLRELSKQSGGKEESPVKRSGAQALLESDLSRMLVNPDGPTMAPLNEVQSAERPLFLVHPIEGSIAAFRTLTAKLSVPCYGLQCTKAAPLDSIQSLAAYYVECVRQVQLEGPYRIAGYSFGACVAFEMCSQLQLAKCPVEYLFLFDGSHSYVAAYTQSYRAKLTPGKESEAETEALCAFIQQFTGIEYNKLLETLLPLSDLEARVDKAVDLITSSHKNVSRDMLHFAASTFYHKLKAADRYVPTSKYHGNVTLLRAKASSEYGDGLGSDYKLHEVCDGKVSVHVIEGDHRTFLEGEGVESISSIIHSSLSEPRVSTREG
InterPro domains: - IPR020843: Enoylreductase domain (domain) [1545-1860]
- IPR006162: Phosphopantetheine attachment site (ptm) [2158-2173]
- IPR014043: Acyl transferase domain (domain) [493-809]
- IPR016035: Acyl transferase/acyl hydrolase/lysophospholipase (homologous_superfamily) [492-777]
- IPR001227: Acyl transferase domain superfamily (homologous_superfamily) [490-788]
- IPR020841: Polyketide synthase, beta-ketoacyl synthase domain (domain) [1-409]
- IPR016039: Thiolase-like (homologous_superfamily) [1-405]
- IPR011032: GroES-like superfamily (homologous_superfamily) [1564-1680]
- IPR020807: Polyketide/metazoan fatty acid synthase-like, dehydratase domain (domain) [827-1012]
- IPR001031: Thioesterase (domain) [2245-2500]
- IPR018201: Beta-ketoacyl synthase, active site (active_site) [152-168]
- IPR032821: Polyketide synthase, C-terminal extension (domain) [362-472]
- IPR014031: Beta-ketoacyl synthase, C-terminal (domain) [243-359]
- IPR016036: Malonyl-CoA ACP transacylase, ACP-binding (homologous_superfamily) [617-673]
- IPR009081: Phosphopantetheine binding ACP domain (domain) [2125-2205]
- IPR020806: Polyketide synthase-like, phosphopantetheine-binding domain (domain) [2131-2189]
- IPR029058: Alpha/Beta hydrolase fold (homologous_superfamily) [2221-2501]
- IPR014030: Beta-ketoacyl synthase-like, N-terminal (domain) [1-237]
- IPR029063: S-adenosyl-L-methionine-dependent methyltransferase superfamily (homologous_superfamily) [1114-1412]
- IPR013968: Polyketide synthase-like, ketoreductase domain (domain) [1892-2070]
Interaction partners: - acetyl-CoA carboxylase
- Beta-alanine-activating enzyme
- Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
- long-chain-fatty-acid--CoA ligase
- Acyl-CoA synthetase family member 3
- Enoyl-[acyl-carrier-protein] reductase, mitochondrial
- Enoyl-[acyl-carrier-protein] reductase, mitochondrial
- 3-oxoacyl-[acyl-carrier-protein] synthase
- S-acyl fatty acid synthase thioesterase, medium chain
- Long-chain-fatty-acid--CoA ligase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016746 (acyltransferase activity), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups), GO:0004312 (fatty acid synthase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0035295 (tube development), GO:0009611 (response to wounding), GO:0009888 (tissue development), GO:0001568 (blood vessel development), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0033554 (cellular response to stress), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0009790 (embryo development), GO:0035239 (tube morphogenesis), GO:0048863 (stem cell differentiation), GO:0048864 (stem cell development), GO:0048568 (embryonic organ development), GO:0048514 (blood vessel morphogenesis), GO:0055123 (digestive system development), GO:0002064 (epithelial cell development), GO:0001944 (vasculature development), GO:0072359 (circulatory system development), GO:0048732 (gland development), GO:0060485 (mesenchyme development), GO:0048762 (mesenchymal cell differentiation), GO:0048565 (digestive tract development), GO:0061008 (hepaticobiliary system development), GO:0007507 (heart development), GO:0009792 (embryo development ending in birth or egg hatching), GO:0014033 (neural crest cell differentiation), GO:0043009 (chordate embryonic development), GO:0001889 (liver development), GO:0014032 (neural crest cell development), GO:0090497 (mesenchymal cell migration), GO:0048706 (embryonic skeletal system development), GO:0001755 (neural crest cell migration)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)