Protein: Epidermal growth factor receptor substrate 15 (A7BFV9)
Organism: Rattus norvegicus (Rat)
Function: Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2
Subcellular location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, clathrin-coated pit
Sequence: MAAAAQLSLTQLSSGNPVYEKYYRQVEAGNTGRVLALDAAAFLKKSGLPDLILGKIWDLADTDGKGVLNKQEFFIALRLVACAQNGLEVSLSSLNLAVPPPRFHDSSSPLLTSGTSVAELPWAVKSEDKAKYDAIFDSLSPVDGFLSGDKVKPVLLNSKLPVEILGRVWELSDIDHDGKLDRDEFAVAMFLVYCALEKEPVPMSLPPALVPPSKRKTWVVSPAEKAKYDEIFLKTDKDMDGYVSGLEVRETFLKTGLPSTLLAHIWALCDTKNCGKLSKDQFALAFHLINQKLIKGIDPPHSLTPEMIPPSDRSSLQKNTIGSSPVADFSAIKELDTLNNEIIDLQREKNNVEQDLKEKEDTVRQRTTEAQDLQDEVQRESLNLQKLQAQKQQVQELLDELDEQKAQLEEQLKEVRKKCAEEAQLISSLKAEITSQESQISTYEEELSKAREELSRLQQETAQLEESVESGKAQLEPLQQHLQDSQQEISSMQMRLAMKDLETDNNQSNWCSSPQSILVNGAADYCSLSTSSSETANLNEHAEGQNNLESEPIHPESSVRSSPEIAPSDVTDESEVVTAADIEKVSPRFDDDKHSKEEDPFNVESSSLTDAVADTNLDFFQSDPFVGSDPFKDDPFGKIDPFGGDPFKGSDPFASDCFFKQTSTDPFATSSTDPFSASSNSSNTSVETWKHNDPFAPGGTVVAATDSATDPFASVFGNESFGDGFADFSTLSKVSNEDPFNPTISSSASSVVIPKPVLEETPSKSEDVPPALPPKTGTPTRPCPPPPGKRPINKLDSSDPFKLNDPFQPFPGNDSPKEKDPDMFCDPFTSSSTTANKEAEPSNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQEDLELAIALSKSEISEA
InterPro domains: - IPR000261: EH domain (domain) [8-317]
- IPR011992: EF-hand domain pair (homologous_superfamily) [16-310]
- IPR018247: EF-Hand 1, calcium-binding site (binding_site) [173-248]
- IPR003903: Ubiquitin interacting motif (conserved_site) [852-897]
- IPR002048: EF-hand domain (domain) [48-289]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0065008 (regulation of biological quality), GO:0023056 (positive regulation of signaling), GO:0031503 (protein-containing complex localization), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0051234 (establishment of localization), GO:0009893 (positive regulation of metabolic process), GO:0023051 (regulation of signaling), GO:0048522 (positive regulation of cellular process), GO:0042127 (regulation of cell population proliferation), GO:0046907 (intracellular transport), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0001919 (regulation of receptor recycling), GO:0006810 (transport), GO:0051649 (establishment of localization in cell), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0099072 (regulation of postsynaptic membrane neurotransmitter receptor levels), GO:0051668 (localization within membrane), GO:0001921 (positive regulation of receptor recycling), GO:0016197 (endosomal transport), GO:0098876 (vesicle-mediated transport to the plasma membrane), GO:0016192 (vesicle-mediated transport), GO:0006897 (endocytosis), GO:0099003 (vesicle-mediated transport in synapse), GO:0032456 (endocytic recycling), GO:0006898 (receptor-mediated endocytosis), GO:0031623 (receptor internalization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0045178 (basal part of cell), GO:0043226 (organelle), GO:0045177 (apical part of cell), GO:0016020 (membrane), GO:0030054 (cell junction), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0012505 (endomembrane system), GO:0009925 (basal plasma membrane), GO:0098590 (plasma membrane region), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0045202 (synapse), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0016324 (apical plasma membrane), GO:0005769 (early endosome), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0098978 (glutamatergic synapse), GO:0097708 (intracellular vesicle)