Protein: Methylosome protein WDR77 (Q99J09)
Organism: Mus musculus (Mouse)
Function: Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones (By similarity). This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles (By similarity). Might play a role in transcription regulation (By similarity). The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain- containing proteins and subsequent localization to the meiotic nuage
Subcellular location: Nucleus. Cytoplasm
Sequence: MRKDTPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGSLLLGVSSLSGRCWVGSLWFFKDPSAAPNEGFCSAGVQTEAGVADLTWVGDKGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVTVLSSGTQAVSGSKDCCIKIWDLAQQVSLNSYRAHAGQVTCVAASPHKDSVFLSCSEDSRILLWDTRCPKPASQMACNASGYLPTALAWHPQQSEVFVFGDENGSVSLVDTKNASCTLSSAVHSQGVTRLVFSPHSVPLLTSLSEDCSLAVLDSSLSEVFRSRAHRDFVRDATWSPLNHSLLTTVGWDHQVIHHVVPLEPLPNPGPDSVVE
InterPro domains: - IPR052139: Methylosome Component WDR77 (family) [10-336]
- IPR036322: WD40-repeat-containing domain superfamily (homologous_superfamily) [79-322]
- IPR015943: WD40/YVTN repeat-like-containing domain superfamily (homologous_superfamily) [21-342]
- IPR001680: WD40 repeat (repeat) [70-326]
- IPR019775: WD40 repeat, conserved site (conserved_site) [140-154]
Interaction partners: - Protein arginine N-methyltransferase 5
- Methylosome subunit pICln
- Serine/threonine-protein kinase RIO1
- Small nuclear ribonucleoprotein Sm D3
- Small nuclear ribonucleoprotein Sm D1
- Small nuclear ribonucleoprotein-associated protein B
- Protein arginine N-methyltransferase 1
- Cyclin-dependent kinase 4
- Coordinator of PRMT5 and differentiation stimulator
- Small nuclear ribonucleoprotein Sm D2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0048869 (cellular developmental process), GO:0050793 (regulation of developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:2000241 (regulation of reproductive process), GO:2000242 (negative regulation of reproductive process), GO:0019222 (regulation of metabolic process), GO:0008283 (cell population proliferation), GO:0050794 (regulation of cellular process), GO:0051241 (negative regulation of multicellular organismal process), GO:0051239 (regulation of multicellular organismal process), GO:0051093 (negative regulation of developmental process), GO:0003006 (developmental process involved in reproduction), GO:0048523 (negative regulation of cellular process), GO:0048522 (positive regulation of cellular process), GO:0042127 (regulation of cell population proliferation), GO:0009888 (tissue development), GO:0030154 (cell differentiation), GO:0008285 (negative regulation of cell population proliferation), GO:0048731 (system development), GO:0050673 (epithelial cell proliferation), GO:0048513 (animal organ development), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0048608 (reproductive structure development), GO:0080090 (regulation of primary metabolic process), GO:0008284 (positive regulation of cell population proliferation), GO:0061458 (reproductive system development), GO:0050680 (negative regulation of epithelial cell proliferation), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0030850 (prostate gland development), GO:0031326 (regulation of cellular biosynthetic process), GO:0048732 (gland development), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0001655 (urogenital system development), GO:0051252 (regulation of RNA metabolic process), GO:0060429 (epithelium development), GO:0050678 (regulation of epithelial cell proliferation), GO:0010468 (regulation of gene expression), GO:0030855 (epithelial cell differentiation), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0002065 (columnar/cuboidal epithelial cell differentiation), GO:0006357 (regulation of transcription by RNA polymerase II), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0002067 (glandular epithelial cell differentiation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043233 (organelle lumen), GO:1990234 (transferase complex), GO:0043229 (intracellular organelle), GO:0034708 (methyltransferase complex), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)