Protein: Bifunctional purine biosynthetic protein PUR2,5 (OpPUR2,5) [Includes: Phosphoribosylamine--glycine ligase (EC 6.3.4.13) (Glycinamide ribonucleotide synthetase) (GAR synthetase) (GARS) (Phosphoribosylglycinamide synthetase); Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1) (AIR synthase) (AIR synthetase) (AIRS) (Phosphoribosyl-aminoimidazole synthetase)] (G8EWC8)
Organism: Pichia angusta (Yeast) (Hansenula polymorpha)
Function: Catalyzes the second and fifth step in the 'de novo' purine biosynthesis pathway; contains phosphoribosylamine--glycine ligase (GARS) and phosphoribosylformylglycinamidine cyclo-ligase (AIRS) activities.
Subcellular location: Cytoplasm, cytosol {ECO:0000250|UniProtKB:P20772}.
Sequence: MEKINVLVVGNGGREHALVWKLAQSPWAKHIFVAPGNGGFSKLENVTSVPIGSSPSDFGSLVDFATKHNVGLVIPGPEQPLVDGITTWFQKAGIPVFGPSAKAARMEGSKTFSKDFMKKHNIPTARYENFTDYEAAKQYIANSSHNLVIKASGIAAGKGVLIPANKQEAYEAIKEIMVNKQFGSAGDEVVIEEFLEGDELSILCISDGYSFVDLPPAQDHKRIGDGDTGLNTGGMGAYSPAPIGTPSLLEKIRKNILKPTIDGMRKDGYPMVGCLFVGVMVTPDGDPKVLEYNVRFGDPETQTVLPLLKSDLLELMLATVEHRLDSVDFQVHADKYSTTVVVAAGGYPESYRKGDEITVKEPLPENTFIFHAGTKEENGKVVTAGGRVIAATAIADTLEEAVKKAYVGVDHISFKDKYNRTDIAHRAFKEKPKNKVSLTYEDAGVSVDAGNQLVEKIKKSVKSTKRPGADSEIGGFGGLFDLQRAGYTDINNTLLVAATDGVGTKLRVAQIMDIHNTVGIDLVAMNVNDLVVQGAEPLMFLDYFATAHLDIKVAADFVEGVADGCKLSGCALVGGETSEMPGMYAPGHYDTNGTAVGAVLKENILPKKDKMNAGDVLLGIASDGVHSNGFSLIRKIIETTDYSYTDPAPWNPKSTIGEEVLIPTRIYVKQLLPATRRGLILGLAHITGGGLVENIPRAIPDNLSAEVDMTTWNVPEIFKWLGKTGGVPINDILKTLNMGIGMVAIVKPENVEEVIKVLKEAGETVYTIGKLVERKDLPGCTIKNSEDLY
InterPro domains: - IPR016185: Pre-ATP-grasp domain superfamily (homologous_superfamily) [4-108]
- IPR020559: Phosphoribosylglycinamide synthetase, conserved site (conserved_site) [295-302]
- IPR020562: Phosphoribosylglycinamide synthetase, N-terminal (domain) [5-107]
- IPR004733: Phosphoribosylformylglycinamidine cyclo-ligase (family) [90-785]
- IPR011761: ATP-grasp fold (domain) [114-321]
- IPR037123: Phosphoribosylglycinamide synthetase, C-domain superfamily (homologous_superfamily) [337-437]
- IPR000115: Phosphoribosylglycinamide synthetase (family) [4-428]
- IPR011054: Rudiment single hybrid motif (homologous_superfamily) [334-428]
- IPR013815: ATP-grasp fold, subdomain 1 (homologous_superfamily) [126-195]
- IPR036921: PurM-like, N-terminal domain superfamily (homologous_superfamily) [438-605]
- IPR010918: PurM-like, C-terminal domain (domain) [613-776]
- IPR020561: Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain (domain) [108-301]
- IPR020560: Phosphoribosylglycinamide synthetase, C-domain (domain) [336-428]
- IPR016188: PurM-like, N-terminal domain (domain) [487-599]
- IPR036676: PurM-like, C-terminal domain superfamily (homologous_superfamily) [606-788]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0016879 (ligase activity, forming carbon-nitrogen bonds)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0055086 (nucleobase-containing small molecule metabolic process), GO:0019637 (organophosphate metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0006793 (phosphorus metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0006753 (nucleoside phosphate metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0019693 (ribose phosphate metabolic process), GO:0018130 (heterocycle biosynthetic process), GO:0090407 (organophosphate biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0072521 (purine-containing compound metabolic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0009259 (ribonucleotide metabolic process), GO:0009123 (nucleoside monophosphate metabolic process), GO:0009117 (nucleotide metabolic process), GO:0046390 (ribose phosphate biosynthetic process), GO:0006163 (purine nucleotide metabolic process), GO:0072522 (purine-containing compound biosynthetic process), GO:1901293 (nucleoside phosphate biosynthetic process), GO:0009126 (purine nucleoside monophosphate metabolic process), GO:0009124 (nucleoside monophosphate biosynthetic process), GO:0009165 (nucleotide biosynthetic process), GO:0006164 (purine nucleotide biosynthetic process), GO:0009260 (ribonucleotide biosynthetic process), GO:0009150 (purine ribonucleotide metabolic process), GO:0009161 (ribonucleoside monophosphate metabolic process), GO:0009152 (purine ribonucleotide biosynthetic process), GO:0009156 (ribonucleoside monophosphate biosynthetic process), GO:0009167 (purine ribonucleoside monophosphate metabolic process), GO:0009127 (purine nucleoside monophosphate biosynthetic process), GO:0046040 (IMP metabolic process), GO:0006188 (IMP biosynthetic process), GO:0009168 (purine ribonucleoside monophosphate biosynthetic process), GO:0006189 ('de novo' IMP biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)