Protein: Spindle pole body-associated protein CIK1 (Chromosome instability and karyogamy protein 1) (Q01649)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: A developmentally regulated protein important for microtubule functions. Targets KAR3 to the cytoplasmic microtubules during mating. Essential for KAR3 function during prophase of meiosis I. Required for interhomolog recombination, synapsis of homologous chromosomes and establishment of a meiosis I spindle.
Subcellular location: Nucleus {ECO:0000269|PubMed:11729143}. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body {ECO:0000269|PubMed:11729143}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:11729143, ECO:0000269|PubMed:25313961}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:8106549}. Note=Localization to the spindle pole body is infrequent in meiotic cells (PubMed:11729143). Localizes to cytoplasmic microtubules during mating (PubMed:8106549). {ECO:0000269|PubMed:11729143, ECO:0000269|PubMed:8106549}.
Sequence: MNNSKIPKLSFHSDPNNVTRDFPKTKRQKVQKREMDMILTPNNNKLNILHSSGSGIRRCYTDDTSATYTKKLTFGGDPKIIERVKNNERKVRKDIDSLLNAISEIEKESVRIHARELPAITLELDAKVKACRELQNEIDGLSTEMDLKDNQCDLQRKNVELSSKNIVSMHAVKVQEFENDLEEELSNAKREWTYKLMEVENLKPDERLTDEMRQLKTEFEEVNRKLFILQNENENECKNYKKELDKKFEIFKKVKNDARIELDGEQERLSKVLKDLQDTHGELKENIKTCRDEFNDFEKRIGEAEVNFHSMELAVVPLKKKLASTSQALTQVQEEKKQVEGEANNWKKKYVNELEKVQQELYTRQNLATSIEEIKGYTRCFAYANERQMPDEFHINYVDRCICENSGEKRVQVFDRVVLEEIHKDHKRLYNECIPFLEKYISKLINCSIIVVSQQPTAPMKKTLLKQLIEQYGENYKMTLNILHLDGSIKHSDVGLDNPTEIRDLSQDEECMNILTLDTKLGKDEESHSMNIYIGSMSTVQLNRELDDAPSVLSHILTKTKQCFVFKINAGENIEKALALAGKLKRTITLPQLD
InterPro domains: - IPR031852: Spindle pole body-associated protein Vik1/Cik1, microtubule binding domain (domain) [358-486]
Interaction partners: - Kinesin-like protein KAR3
- Kinesin-like protein KIP1
- Kinesin-like protein KIP3
- Spindle pole body-associated protein VIK1
- Kinesin-like protein KIP2
- Protein BIM1
- Cell division control protein KAR1
- Kinesin-like protein CIN8
- Cell division control protein 31
- Shugoshin
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0008092 (cytoskeletal protein binding), GO:0015631 (tubulin binding), GO:0008017 (microtubule binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0007017 (microtubule-based process), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:0016043 (cellular component organization), GO:0007059 (chromosome segregation), GO:0000226 (microtubule cytoskeleton organization), GO:0044085 (cellular component biogenesis), GO:1903047 (mitotic cell cycle process), GO:0000278 (mitotic cell cycle), GO:0007051 (spindle organization), GO:1902850 (microtubule cytoskeleton organization involved in mitosis), GO:0022607 (cellular component assembly), GO:0051225 (spindle assembly), GO:0098813 (nuclear chromosome segregation), GO:0007052 (mitotic spindle organization), GO:0006996 (organelle organization), GO:0000070 (mitotic sister chromatid segregation), GO:0140014 (mitotic nuclear division), GO:0070925 (organelle assembly), GO:0007010 (cytoskeleton organization), GO:0048285 (organelle fission), GO:0000819 (sister chromatid segregation), GO:0090307 (mitotic spindle assembly), GO:0051276 (chromosome organization), GO:0000280 (nuclear division), GO:0140694 (non-membrane-bounded organelle assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005875 (microtubule associated complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005816 (spindle pole body), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0015630 (microtubule cytoskeleton)