Protein: Alcohol dehydrogenase class-3 (EC 1.1.1.1) (Alcohol dehydrogenase 2) (Alcohol dehydrogenase 5) (Alcohol dehydrogenase B2) (ADH-B2) (Alcohol dehydrogenase class-III) (Glutathione-dependent formaldehyde dehydrogenase) (FALDH) (FDH) (GSH-FDH) (EC 1.1.1.-) (S-(hydroxymethyl)glutathione dehydrogenase) (EC 1.1.1.284) (P12711)
Organism: Rattus norvegicus (Rat)
Function: Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Class-III ADH is remarkably ineffective in oxidizing ethanol. Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage.
Subcellular location: Cytoplasm {ECO:0000305}.
Sequence: MANQVIRCKAAVAWEAGKPLSIEEIEVAPPQAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKPILHFMGTSTFSEYTVVADISVAKIDPSAPLDKVCLLGCGISTGYGAAVNTAKVEPGSTCAVFGLGGVGLAVIMGCKVAGASRIIGIDINKDKFAKAKEFGATECINPQDFSKSIQEVLIEMTDGGVDFSFECIGNVKVMRSALEAAHKGWGVSVVVGVAASGEEISTRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTGNLSFDQINKAFDLMHSGNSIRTVLKM
InterPro domains: - IPR036291: NAD(P)-binding domain superfamily (homologous_superfamily) [165-339]
- IPR013149: Alcohol dehydrogenase-like, C-terminal (domain) [202-331]
- IPR011032: GroES-like superfamily (homologous_superfamily) [3-372]
- IPR013154: Alcohol dehydrogenase-like, N-terminal (domain) [32-160]
- IPR014183: Alcohol dehydrogenase class III (family) [6-374]
- IPR002328: Alcohol dehydrogenase, zinc-type, conserved site (conserved_site) [66-80]
Interaction partners: - S-formylglutathione hydrolase
- Aldehyde dehydrogenase, dimeric NADP-preferring
- Aldehyde dehydrogenase 1A1
- Aldehyde dehydrogenase
- Aldehyde dehydrogenase, mitochondrial
- Aldehyde dehydrogenase family 3 member A2
- Alpha-aminoadipic semialdehyde dehydrogenase
- 4-trimethylaminobutyraldehyde dehydrogenase
- Aldehyde dehydrogenase X, mitochondrial
- Aldehyde dehydrogenase family 3 member B1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0097159 (organic cyclic compound binding), GO:0016491 (oxidoreductase activity), GO:0043178 (alcohol binding), GO:0016614 (oxidoreductase activity, acting on CH-OH group of donors), GO:1901265 (nucleoside phosphate binding), GO:0000166 (nucleotide binding), GO:0016616 (oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor), GO:0017076 (purine nucleotide binding), GO:0018455 (alcohol dehydrogenase [NAD(P)+] activity), GO:0004745 (NAD-retinol dehydrogenase activity), GO:0030554 (adenyl nucleotide binding), GO:0051287 (NAD binding), GO:0004022 (alcohol dehydrogenase (NAD+) activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0048856 (anatomical structure development), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0009719 (response to endogenous stimulus), GO:1901615 (organic hydroxy compound metabolic process), GO:0006066 (alcohol metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901700 (response to oxygen-containing compound), GO:1901576 (organic substance biosynthetic process), GO:0031099 (regeneration), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:0006091 (generation of precursor metabolites and energy), GO:0006081 (cellular aldehyde metabolic process), GO:0048513 (animal organ development), GO:0034308 (primary alcohol metabolic process), GO:0048545 (response to steroid hormone), GO:0031100 (animal organ regeneration), GO:0006117 (acetaldehyde metabolic process), GO:0046184 (aldehyde biosynthetic process), GO:0014070 (response to organic cyclic compound), GO:1901654 (response to ketone), GO:0033993 (response to lipid), GO:0006067 (ethanol metabolic process), GO:0032570 (response to progesterone)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)