Protein: Protein unc-13 homolog B (Munc13-2) (munc13) (Q9Z1N9)
Organism: Mus musculus (Mouse)
Function: Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP) (By similarity). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. In collaboration with UNC13A, facilitates neuronal dense core vesicles fusion as well as controls the location and efficiency of their synaptic release.
Subcellular location: Cytoplasm. Membrane; Peripheral membrane protein. Golgi apparatus. Synapse {ECO:0000250}. Note=Localized to synapses (By similarity). Translocates to the Golgi in response to phorbol ester binding. {ECO:0000250}.
Sequence: MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARYWTYKLEQINALADDNEYSSQEESQRKPLPTAAAQCCHWTYLGWGEHQTFEDPDSAVDDRDSDYRSETSNSAPPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVSQGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDATIHPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAGGLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLQEQPLEEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS
InterPro domains: - IPR014772: Mammalian uncoordinated homology 13, domain 2 (domain) [1274-1416]
- IPR035892: C2 domain superfamily (homologous_superfamily) [1-1567]
- IPR046349: C1-like domain superfamily (homologous_superfamily) [480-539]
- IPR014770: Munc13 homology 1 (domain) [1024-1167]
- IPR000008: C2 domain (domain) [1-1559]
- IPR002219: Protein kinase C-like, phorbol ester/diacylglycerol-binding domain (domain) [489-539]
- IPR010439: MUN domain (domain) [929-1416]
- IPR027080: Protein Unc-13 (family) [332-1601]
- IPR037302: Protein Unc-13, C2B domain (domain) [612-738]
Interaction partners: - Regulating synaptic membrane exocytosis protein 1
- DNA endonuclease RBBP8
- Protein bassoon
- Ras-related protein Rab-3A
- Calmodulin-1
- Synaptosomal-associated protein 25
- Syntaxin-1A
- Vesicle-associated membrane protein 2
- Synaptotagmin-1
- Protein unc-13 homolog C
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0005516 (calmodulin binding)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0042592 (homeostatic process), GO:0023052 (signaling), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0022406 (membrane docking), GO:0051641 (cellular localization), GO:0048856 (anatomical structure development), GO:0023056 (positive regulation of signaling), GO:0042221 (response to chemical), GO:0032879 (regulation of localization), GO:0140029 (exocytic process), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0051050 (positive regulation of transport), GO:0007267 (cell-cell signaling), GO:0048878 (chemical homeostasis), GO:0065008 (regulation of biological quality), GO:0019725 (cellular homeostasis), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0021700 (developmental maturation), GO:0140352 (export from cell), GO:0007154 (cell communication), GO:0051640 (organelle localization), GO:0048522 (positive regulation of cellular process), GO:0006904 (vesicle docking involved in exocytosis), GO:1903530 (regulation of secretion by cell), GO:1903829 (positive regulation of protein localization), GO:0051049 (regulation of transport), GO:0051047 (positive regulation of secretion), GO:0044085 (cellular component biogenesis), GO:0046887 (positive regulation of hormone secretion), GO:0060341 (regulation of cellular localization), GO:0033500 (carbohydrate homeostasis), GO:0051649 (establishment of localization in cell), GO:0140056 (organelle localization by membrane tethering), GO:0001505 (regulation of neurotransmitter levels), GO:0060627 (regulation of vesicle-mediated transport), GO:1901700 (response to oxygen-containing compound), GO:0051648 (vesicle localization), GO:0010817 (regulation of hormone levels), GO:0010033 (response to organic substance), GO:0046883 (regulation of hormone secretion), GO:0055082 (intracellular chemical homeostasis), GO:0099536 (synaptic signaling), GO:0016082 (synaptic vesicle priming), GO:0010646 (regulation of cell communication), GO:0070887 (cellular response to chemical stimulus), GO:0006810 (transport), GO:0032940 (secretion by cell), GO:0016043 (cellular component organization), GO:0010647 (positive regulation of cell communication), GO:0051222 (positive regulation of protein transport), GO:0023061 (signal release), GO:1903532 (positive regulation of secretion by cell), GO:0051588 (regulation of neurotransmitter transport), GO:0009743 (response to carbohydrate), GO:0043254 (regulation of protein-containing complex assembly), GO:0046928 (regulation of neurotransmitter secretion), GO:0071310 (cellular response to organic substance), GO:1904951 (positive regulation of establishment of protein localization), GO:0070201 (regulation of establishment of protein localization), GO:0002793 (positive regulation of peptide secretion), GO:0009914 (hormone transport), GO:0046903 (secretion), GO:0032880 (regulation of protein localization), GO:0045921 (positive regulation of exocytosis), GO:0051650 (establishment of vesicle localization), GO:0050714 (positive regulation of protein secretion), GO:0022607 (cellular component assembly), GO:0006836 (neurotransmitter transport), GO:0099504 (synaptic vesicle cycle), GO:0051223 (regulation of protein transport), GO:0006887 (exocytosis), GO:0090276 (regulation of peptide hormone secretion), GO:0090087 (regulation of peptide transport), GO:0042593 (glucose homeostasis), GO:0099643 (signal release from synapse), GO:0099537 (trans-synaptic signaling), GO:0051046 (regulation of secretion), GO:0090277 (positive regulation of peptide hormone secretion), GO:0016192 (vesicle-mediated transport), GO:0090277 (positive regulation of peptide hormone secretion), GO:0043933 (protein-containing complex organization), GO:0006996 (organelle organization), GO:0050708 (regulation of protein secretion), GO:0001678 (intracellular glucose homeostasis), GO:1901701 (cellular response to oxygen-containing compound), GO:0016079 (synaptic vesicle exocytosis), GO:0048791 (calcium ion-regulated exocytosis of neurotransmitter), GO:0032024 (positive regulation of insulin secretion), GO:0050796 (regulation of insulin secretion), GO:0065003 (protein-containing complex assembly), GO:0016050 (vesicle organization), GO:0071322 (cellular response to carbohydrate stimulus), GO:0071333 (cellular response to glucose stimulus), GO:0034284 (response to monosaccharide), GO:0002791 (regulation of peptide secretion), GO:0042886 (amide transport), GO:0098916 (anterograde trans-synaptic signaling), GO:0015833 (peptide transport), GO:0002790 (peptide secretion), GO:0099003 (vesicle-mediated transport in synapse), GO:0071692 (protein localization to extracellular region), GO:0030073 (insulin secretion), GO:0045055 (regulated exocytosis), GO:0030072 (peptide hormone secretion), GO:0007268 (chemical synaptic transmission), GO:0071326 (cellular response to monosaccharide stimulus), GO:0017156 (calcium-ion regulated exocytosis), GO:0017158 (regulation of calcium ion-dependent exocytosis), GO:0009746 (response to hexose), GO:0071331 (cellular response to hexose stimulus), GO:0009749 (response to glucose)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0150034 (distal axon), GO:0044306 (neuron projection terminus), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0030054 (cell junction), GO:0098794 (postsynapse), GO:0098793 (presynapse), GO:0071944 (cell periphery), GO:0045211 (postsynaptic membrane), GO:0005886 (plasma membrane), GO:0098590 (plasma membrane region), GO:0120025 (plasma membrane bounded cell projection), GO:0043679 (axon terminus), GO:0045202 (synapse), GO:0043195 (terminal bouton), GO:0043005 (neuron projection), GO:0097060 (synaptic membrane), GO:0030424 (axon)