Protein: Chronophin (EC 3.1.3.16) (EC 3.1.3.74) (Pyridoxal phosphate phosphatase) (PLP phosphatase) (Q96GD0)
Organism: Homo sapiens (Human)
Function: Functions as a pyridoxal phosphate (PLP) phosphatase, which also catalyzes the dephosphorylation of pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), with order of substrate preference PLP > PNP > PMP and therefore plays a role in vitamin B6 metabolism. Also functions as a protein serine phosphatase that specifically dephosphorylates 'Ser-3' in proteins of the actin-depolymerizing factor (ADF)/cofilin family like CFL1 and DSTN. Thereby, regulates cofilin-dependent actin cytoskeleton reorganization, being required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phosphothreonines in LIMK1. Does not dephosphorylate peptides containing phosphotyrosine.
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:15580268}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:15580268}. Cell projection, ruffle membrane {ECO:0000269|PubMed:15580268}; Peripheral membrane protein {ECO:0000269|PubMed:15580268}; Cytoplasmic side {ECO:0000269|PubMed:15580268}. Cell projection, lamellipodium membrane {ECO:0000269|PubMed:15580268}; Peripheral membrane protein {ECO:0000269|PubMed:15580268}; Cytoplasmic side {ECO:0000269|PubMed:15580268}. Cell membrane {ECO:0000269|PubMed:15580268}; Peripheral membrane protein {ECO:0000269|PubMed:15580268}; Cytoplasmic side {ECO:0000269|PubMed:15580268}. Note=Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Diffusely distributed throughout the cytosol during pro-metaphase and metaphase. Detected at the dynamic cell poles during telophase. Detected at the cleavage furrow and contractile ring during cytokinesis. Transiently detected at the plasma membrane in late stages of cytokinesis. Detected at the midbody. {ECO:0000269|PubMed:15580268}.
Sequence: MARCERLRGAALRDVLGRAQGVLFDCDGVLWNGERAVPGAPELLERLARAGKAALFVSNNSRRARPELALRFARLGFGGLRAEQLFSSALCAARLLRQRLPGPPDAPGAVFVLGGEGLRAELRAAGLRLAGDPSAGDGAAPRVRAVLVGYDEHFSFAKLREACAHLRDPECLLVATDRDPWHPLSDGSRTPGTGSLAAAVETASGRQALVVGKPSPYMFECITENFSIDPARTLMVGDRLETDILFGHRCGMTTVLTLTGVSRLEEAQAYLAAGQHDLVPHYYVESIADLTEGLED
InterPro domains: - IPR006349: 2-phosphoglycolate phosphatase, eukaryotic (family) [18-290]
- IPR006357: HAD-superfamily hydrolase, subfamily IIA (family) [1-296]
- IPR023214: HAD superfamily (homologous_superfamily) [20-289]
- IPR036412: HAD-like superfamily (homologous_superfamily) [20-291]
Interaction partners: - Pyridoxal kinase
- Pyridoxine-5'-phosphate oxidase
- Aldehyde oxidase
- Pyridoxal phosphate phosphatase PHOSPHO2
- Cofilin-2
- Cofilin-1
- LIM domain kinase 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0042802 (identical protein binding), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:0046983 (protein dimerization activity), GO:0042803 (protein homodimerization activity), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0016462 (pyrophosphatase activity), GO:0004427 (inorganic diphosphate phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044282 (small molecule catabolic process), GO:0046483 (heterocycle metabolic process), GO:0044248 (cellular catabolic process), GO:0019637 (organophosphate metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0006766 (vitamin metabolic process), GO:1901575 (organic substance catabolic process), GO:0006081 (cellular aldehyde metabolic process), GO:0006793 (phosphorus metabolic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0046185 (aldehyde catabolic process), GO:1901616 (organic hydroxy compound catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0006767 (water-soluble vitamin metabolic process), GO:0019439 (aromatic compound catabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0046700 (heterocycle catabolic process), GO:0009111 (vitamin catabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0046434 (organophosphate catabolic process), GO:0072524 (pyridine-containing compound metabolic process), GO:0042822 (pyridoxal phosphate metabolic process), GO:0016311 (dephosphorylation), GO:0042816 (vitamin B6 metabolic process), GO:0072526 (pyridine-containing compound catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0043226 (organelle), GO:0005576 (extracellular region), GO:0005615 (extracellular space), GO:0070062 (extracellular exosome), GO:0043227 (membrane-bounded organelle), GO:0043230 (extracellular organelle), GO:0065010 (extracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:1903561 (extracellular vesicle)