Protein: Glutamate synthase 1 [NADH], chloroplastic (EC 1.4.1.14) (NADH-dependent glutamate synthase 1) (NADH-GOGAT 1) (Q9LV03)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Involved in glutamate biosynthesis. Required for non-photorespiratory ammonium assimilation. Probably involved in primary ammonium assimilation in roots.
Subcellular location: Plastid, chloroplast {ECO:0000269|PubMed:18431481}.
Sequence: MSAASSSSVLHLRTNQQLLSLRSLKNSTSVASQLAVTSGVSRRRSCTARCSVKKPVIPESPFLGTRVRRSGSETLQFWRSDGPGRSAKLRTVVKSSFSAVPEKPLGLYDPSYDKDSCGVGFVAELSGETTRKTVTDSLEMLIRMTHRGACGCESNTGDGAGILVGLPHDFYAEAATELGFVLPSAGNYAVGMFFLPTVESRREESKNVFTKVAESLGHSVLGWRLVPTDNSGLGNSALQTEPIIAQVFLTPTTKSKADFEQQMYILRRVSMVAIRAALNLQHGAMKDFYICSLSSRTIVYKGQLKPDQLKDYYYADLGSERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLKCNELGLSKKELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNIDPSRKEFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEVGVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSLARPYGEWLKRQKIELKDIIESVPEAERIAPSISGVVPASNDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKDGSEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLKIEEMEAIKKMNYRGWRTKVLDITYAKERGTKGLEETLDRICDEANEAIKEGYTLLVLSDRAFSATRVAVSSLMAVGAVHHHLVKTLARTQVGLVVESAEPREVHHFCTLVGFGADAICPYLAVEAVYRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIQKCFAGTPSRVEGATFEMLARDGLQLHELAFPTRGYAPGSAEASALTNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSKRINELNKQSNLRGLMKFKDADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKLGGKSNTGEGGELPSRMEPLADGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEEVREIMSGLGFRTVTEMIGRADMLELDREVVKNNDKLENIDLSLLLRPAAEIRPGAAQYCVQKQDHGLDMALDQELIALSKSALEKSLPVYIETPICNVNRAVGTMLSHEVTKRYHLTGLPKDTIHIKFTGSAGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFSVRNSGAKAVVEGLGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFNTRCNLELVDLDKVEDEEDKMTLKMMIQQHQRHTNSQLAQEVLADFENLLPKFIKVFPRDYKRVLSAMKHEEVSKQAIERASEEADETEEKELEEKDAFAELKNMAAASSKEEMSGNGVAAEARPSKVDNAVKNGGFIAYEREGVKYRDPNVRLNDWNEVMEESKPGPLLTTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFEEGWMVPRPPLKRTGKKVAIIGSGPAGLAAADQLNKMGHLVTVYERSDRIGGLMMYGVPNMKTDKIDVVQRRVDLMTKEGINFVVNANIGKDPSYSLDGLKEENDAIVLAVGSTKPRDLPVPGRDLSGVHFAMEFLHANTKSLLDSNHEDGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQEATTKFGKDPRTYEVLTKRFIGDDNGNVKGLELVRVSWEKDETGRFQFKEIEGSEEIIEADLVFLAMGFLGPEPTLAEKLGLECDNRSNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWAISEGRQAADQVDKFLTKTDDDEDAKLQQDLNQMKHNTITN
InterPro domains: - IPR051394: Glutamate Synthase (family) [831-2203]
- IPR029055: Nucleophile aminohydrolases, N-terminal (homologous_superfamily) [117-556]
- IPR006982: Glutamate synthase, central-N (domain) [594-882]
- IPR002932: Glutamate synthase domain (domain) [949-1332]
- IPR006005: Glutamate synthase, NADH/NADPH, small subunit 1 (family) [1701-2185]
- IPR012220: Glutamate synthase, eukaryotic (family) [24-2206]
- IPR036485: Glutamate synthase, alpha subunit, C-terminal domain superfamily (homologous_superfamily) [1370-1646]
- IPR009051: Alpha-helical ferredoxin (homologous_superfamily) [1695-1845]
- IPR028261: Dihydroprymidine dehydrogenase domain II (domain) [1724-1833]
- IPR023753: FAD/NAD(P)-binding domain (domain) [1846-2172]
- IPR017932: Glutamine amidotransferase type 2 domain (domain) [117-544]
- IPR013785: Aldolase-type TIM barrel (homologous_superfamily) [570-1370]
- IPR036188: FAD/NAD(P)-binding domain superfamily (homologous_superfamily) [1954-2116]
- IPR002489: Glutamate synthase, alpha subunit, C-terminal (domain) [1378-1627]
Interaction partners: - NADPH:adrenodoxin oxidoreductase, mitochondrial
- Glutamine synthetase cytosolic isozyme 1-3
- Glutamine synthetase cytosolic isozyme 1-2
- Glutamine synthetase cytosolic isozyme 1-1
- Glutamine synthetase, chloroplastic/mitochondrial
- Glutamine synthetase cytosolic isozyme 1-5
- Glutamine synthetase cytosolic isozyme 1-4
- Glutamate dehydrogenase 1
- Glutamate dehydrogenase 2
- Probable glutamate dehydrogenase 3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0016491 (oxidoreductase activity), GO:0003676 (nucleic acid binding), GO:0016638 (oxidoreductase activity, acting on the CH-NH2 group of donors), GO:0003723 (RNA binding), GO:0003729 (mRNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0044281 (small molecule metabolic process), GO:0009719 (response to endogenous stimulus), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0003006 (developmental process involved in reproduction), GO:0044238 (primary metabolic process), GO:0009791 (post-embryonic development), GO:0048316 (seed development), GO:0099402 (plant organ development), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0048731 (system development), GO:0044249 (cellular biosynthetic process), GO:0009790 (embryo development), GO:0048608 (reproductive structure development), GO:0006520 (amino acid metabolic process), GO:1901700 (response to oxygen-containing compound), GO:0006520 (amino acid metabolic process), GO:0009793 (embryo development ending in seed dormancy), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:1901566 (organonitrogen compound biosynthetic process), GO:0048367 (shoot system development), GO:0061458 (reproductive system development), GO:0048364 (root development), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0033993 (response to lipid), GO:1901605 (alpha-amino acid metabolic process), GO:0010154 (fruit development), GO:0022622 (root system development), GO:0019752 (carboxylic acid metabolic process), GO:1901607 (alpha-amino acid biosynthetic process), GO:0009064 (glutamine family amino acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0043650 (dicarboxylic acid biosynthetic process), GO:0009084 (glutamine family amino acid biosynthetic process), GO:0006561 (proline biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0009532 (plastid stroma), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0005737 (cytoplasm), GO:0031967 (organelle envelope), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009570 (chloroplast stroma), GO:0009526 (plastid envelope), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle), GO:0009941 (chloroplast envelope)