Protein: E3 ubiquitin-protein ligase RING1 (EC 2.3.2.27) (RING-type E3 ubiquitin transferase RING1) (Sex comb extra protein) (dRING protein) (dRING1) (Q9VB08)
Organism: Drosophila melanogaster (Fruit fly)
Function: E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-118' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-118' ubiquitination gives a specific tag for epigenetic transcriptional repression. Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. PcG complexes act via modification of histones, such as methylation, deacetylation, ubiquitination rendering chromatin heritably changed in its expressibility. May play a role in meiotic sister chromatid cohesion.
Subcellular location: Nucleus. Chromosome. Note=Colocalizes with ubiquitinated histone H2A. Colocalizes with Pc, Pcl, Psc, ph at many sites on polytene chromosomes. Colocalizes with ORD on the chromatin of primary spermatocytes during G2 of meiosis.
Sequence: MTSLDPAPNKTWELSLYELQRKPQEVITDSTEIAVSPRSLHSELMCPICLDMLKKTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSKRSLRADPNFDLLISKIYPSREEYEAIQEKVMAKFNQTQSQQALVNSINEGIKLQSQNRPQRFRTKGGGGGGGGGGNGNGAANVAAPPAPGAPTAVGRNASNQMHVHDTASNDSNSNTNSIDRENRDPGHSGTSAASAITSASNAAPSSSANSGASTSATRMQVDDASNPPSVRSTPSPVPSNSSSSKPKRAMSVLTSERSEESESDSQMDCRTEGDSNIDTEGEGNGELGINDEIELVFKPHPTEMSADNQLIRALKENCVRYIKTTANATVDHLSKYLAMRLTLDLGADLPEACRVLNFCIYVAPQPQQLVILNGNQTLHQVNDKFWKVNKPMEMYYSWKKT
InterPro domains: - IPR017907: Zinc finger, RING-type, conserved site (conserved_site) [62-71]
- IPR042741: RING1 , RING finger, HC subclass (domain) [41-110]
- IPR043540: E3 ubiquitin-protein ligase RING1/RING2 (family) [5-434]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [5-116]
- IPR032443: RAWUL domain (domain) [354-431]
- IPR001841: Zinc finger, RING-type (domain) [46-86]
Interaction partners: - Polycomb group protein Psc
- Polycomb group protein Pc
- Protein suppressor 2 of zeste
- GEO03726p1
- JmjC domain-containing histone demethylation protein 1
- Polycomb protein esc
- Polyhomeotic-proximal chromatin protein
- Histone-lysine N-methyltransferase E
- Polycomb protein Su
- Polycomb protein Sfmbt
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0003682 (chromatin binding), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0019787 (ubiquitin-like protein transferase activity), GO:0004842 (ubiquitin-protein transferase activity), GO:0061659 (ubiquitin-like protein ligase activity), GO:0061630 (ubiquitin protein ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0032501 (multicellular organismal process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0019222 (regulation of metabolic process), GO:0007389 (pattern specification process), GO:0050794 (regulation of cellular process), GO:0007049 (cell cycle), GO:0065009 (regulation of molecular function), GO:0048523 (negative regulation of cellular process), GO:0009892 (negative regulation of metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0001709 (cell fate determination), GO:0009888 (tissue development), GO:0003002 (regionalization), GO:0030154 (cell differentiation), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0044092 (negative regulation of molecular function), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0000278 (mitotic cell cycle), GO:0022008 (neurogenesis), GO:0007399 (nervous system development), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0051253 (negative regulation of RNA metabolic process), GO:0030182 (neuron differentiation), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0010468 (regulation of gene expression), GO:0043433 (negative regulation of DNA-binding transcription factor activity), GO:0048699 (generation of neurons), GO:2001141 (regulation of RNA biosynthetic process), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0006357 (regulation of transcription by RNA polymerase II), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0045892 (negative regulation of DNA-templated transcription), GO:0000122 (negative regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:1902494 (catalytic complex), GO:0140513 (nuclear protein-containing complex), GO:0140535 (intracellular protein-containing complex), GO:1990234 (transferase complex), GO:0000151 (ubiquitin ligase complex), GO:0000152 (nuclear ubiquitin ligase complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0031519 (PcG protein complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0035102 (PRC1 complex), GO:0005634 (nucleus)