Protein: Eukaryotic translation initiation factor 2-alpha kinase 2 (EC 2.7.11.1) (Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase) (Heme-regulated inhibitor 2) (Q9UTE5)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Mediates down-regulation of protein synthesis in response to stress conditions by the phosphorylation of the alpha subunit of eIF-2 (tif211) on 'Ser-52'. Protein synthesis is inhibited at the level of initiation. Activity is inhibited in the presence of heme.
Sequence: MRFFNAHKSAEDGNYSETTNAISLKNERQSRDLSVNKHGRMLLTALLENFCQLYDNNPAKSKRLFALICHTLQKIGILEEEYIEELAAVRSNYQDALHHLILQAREAIRLDDAEERLLALPDPTNVFEKFPQETALSKFSVDGLNVRQFRFRDLTLESWLQSRNSRYIEDFEEYSLLGRGGFGSVYHVRNKIDGAEYAMKKINSTFQQMSYSKIFREIKCLAKMDHPNVIRYFSSWVESTTEATQMPIVMSKNSSRVLGTSSYCDVNGMDSIIFEPTESSALTDDILFAEDPGTESIISTSRKSSYSSTTESSNFENLESPRNLHDSNVSTFNNTTILDDDYSSSMHISKTGPTHSFIIYIQMQLCFDDLESYLIRRNHFLSFPLCKEQIQLHTDLFRMIINGVMYVHEGVNLIHRDIKPSNIFLAKSLPEDRGSVPLISYNDKNDLKEYITPKIGDFGLVVENKKNTETALSFLERNHLPNLQDETQHIGTATYAAPELLDAMSSQHNKFTKKIDTFSLGMVLFELLHPFQTNMERATKLQDLRRGNLPEEFVEQHICESSLILWMTAKDPTKRPSLLEVLNCGLLLPNQVSMPNISNIVSTNHLDVETQMKLIMDENQRLREQIAVLRSRIQHLETR
InterPro domains: - IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [165-595]
- IPR050339: Cell Cycle and Stress Response Kinase (family) [52-599]
- IPR008271: Serine/threonine-protein kinase, active site (active_site) [413-425]
- IPR054521: Heme-regulated eIF-2-alpha kinase, helical domain (domain) [37-112]
- IPR017441: Protein kinase, ATP binding site (binding_site) [177-200]
- IPR000719: Protein kinase domain (domain) [171-588]
Interaction partners: - Eukaryotic translation initiation factor 2 subunit alpha
- eIF-2-alpha kinase GCN2
- Eukaryotic translation initiation factor 2-alpha kinase 1
- Elongation factor 3
- ATP phosphoribosyltransferase
- mRNA export factor elf1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0045182 (translation regulator activity), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0000003 (reproduction), GO:0019953 (sexual reproduction), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0043934 (sporulation), GO:0009653 (anatomical structure morphogenesis), GO:0042221 (response to chemical), GO:0032505 (reproduction of a single-celled organism), GO:0051321 (meiotic cell cycle), GO:0050794 (regulation of cellular process), GO:0007049 (cell cycle), GO:0051716 (cellular response to stimulus), GO:0022413 (reproductive process in single-celled organism), GO:1903046 (meiotic cell cycle process), GO:0003006 (developmental process involved in reproduction), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0030437 (ascospore formation), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0070887 (cellular response to chemical stimulus), GO:0030435 (sporulation resulting in formation of a cellular spore), GO:0010033 (response to organic substance), GO:0034293 (sexual sporulation), GO:0043935 (sexual sporulation resulting in formation of a cellular spore), GO:0071310 (cellular response to organic substance), GO:0000749 (response to pheromone triggering conjugation with cellular fusion), GO:0000165 (MAPK cascade), GO:0000750 (pheromone-dependent signal transduction involved in conjugation with cellular fusion), GO:0071444 (cellular response to pheromone)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)