Protein: Vitamin B12 transporter BtuB (Cobalamin receptor) (Outer membrane cobalamin translocator) (P06129)
Organism: Escherichia coli (strain K12)
Function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB. Is also a receptor for bacteriophages BF23 and C1, and for A and E colicins.
Subcellular location: Cell outer membrane {ECO:0000255|HAMAP-Rule:MF_01531, ECO:0000269|PubMed:12595710, ECO:0000269|PubMed:12652322, ECO:0000269|PubMed:2687240, ECO:0000269|PubMed:2982793}; Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01531, ECO:0000269|PubMed:12595710, ECO:0000269|PubMed:12652322, ECO:0000269|PubMed:2687240, ECO:0000269|PubMed:2982793}.
Sequence: MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLAPTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDGVRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEISAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFLSKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRYNGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAGVDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQTSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVNWRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARNAITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF
InterPro domains: - IPR036942: TonB-dependent receptor-like, beta-barrel domain superfamily (homologous_superfamily) [151-614]
- IPR010917: TonB-dependent receptor, conserved site (conserved_site) [597-614]
- IPR010101: TonB-dependent vitamin B12 transporter BtuB (family) [2-614]
- IPR039426: TonB-dependent receptor-like (family) [4-614]
- IPR037066: TonB-dependent receptor, plug domain superfamily (homologous_superfamily) [27-150]
- IPR000531: TonB-dependent receptor-like, beta-barrel (domain) [170-588]
- IPR010916: TonB box, conserved site (conserved_site) [1-33]
- IPR012910: TonB-dependent receptor, plug domain (domain) [41-146]
Interaction partners: - Protein TonB
- Outer membrane porin F
- Biopolymer transport protein ExbD
- Biopolymer transport protein ExbB
- Tol-Pal system protein TolB
- Vitamin B12-binding protein
- Glutamate racemase
- Vitamin B12 import system permease protein BtuC
- Nucleoside-specific channel-forming protein Tsx
- Outer membrane protein A
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity), GO:0015343 (siderophore-iron transmembrane transporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0051234 (establishment of localization), GO:0006810 (transport), GO:0071702 (organic substance transport), GO:0006811 (monoatomic ion transport), GO:0015850 (organic hydroxy compound transport), GO:0006812 (monoatomic cation transport), GO:0015711 (organic anion transport), GO:0030001 (metal ion transport), GO:0000041 (transition metal ion transport), GO:0006826 (iron ion transport), GO:1901678 (iron coordination entity transport), GO:0015891 (siderophore transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0031975 (envelope), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0030312 (external encapsulating structure), GO:0009279 (cell outer membrane), GO:0019867 (outer membrane), GO:0030313 (cell envelope)