Protein: Endonuclease 4 (P0A6C1)
Organism: Escherichia coli (strain K12)
Function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin
Sequence: MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPLTTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLGLSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEHLAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLNDAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLKAQQTEKAVA
InterPro domains: - IPR013022: Xylose isomerase-like, TIM barrel domain (domain) [20-277]
- IPR001719: AP endonuclease 2 (family) [1-282]
- IPR018246: AP endonuclease 2, zinc binding site (binding_site) [69-231]
- IPR036237: Xylose isomerase-like superfamily (homologous_superfamily) [1-277]
Interaction partners: - Exodeoxyribonuclease III
- Endonuclease III
- Endonuclease III
- Uncharacterized sugar kinase YeiI
- Formamidopyrimidine-DNA glycosylase
- Superoxide dismutase [Mn]
- UvrABC system protein A
- Uracil-DNA glycosylase
- Adenine DNA glycosylase
- Endonuclease V
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0043167 (ion binding), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0043169 (cation binding), GO:0140097 (catalytic activity, acting on DNA), GO:0046872 (metal ion binding), GO:0004518 (nuclease activity), GO:0042578 (phosphoric ester hydrolase activity), GO:0003906 (DNA-(apurinic or apyrimidinic site) endonuclease activity), GO:0004536 (deoxyribonuclease activity), GO:0016791 (phosphatase activity), GO:0004527 (exonuclease activity), GO:0008081 (phosphoric diester hydrolase activity), GO:0046914 (transition metal ion binding), GO:0004529 (DNA exonuclease activity), GO:0004519 (endonuclease activity), GO:0016895 (DNA exonuclease activity, producing 5'-phosphomonoesters), GO:0008408 (3'-5' exonuclease activity), GO:0008270 (zinc ion binding), GO:0016796 (exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters), GO:0008296 (3'-5'-DNA exonuclease activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0033554 (cellular response to stress), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0006259 (DNA metabolic process), GO:0006281 (DNA repair), GO:0006284 (base-excision repair)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)