Protein: Trimethylguanosine synthase (A0A8M1RLP2)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation
Subcellular location: Cytoplasm. Nucleus, Cajal body. Nucleus, nucleolus
Sequence: MMIDYGERNVVPLADIIFYQGEETEMTIHCLCSRAYVNDRELYRSDFKAISSQADSQVQGDVETNDGEQLLEDLNCSVDEDEEEEEEEEDAAVDEETLLMASLGLPVQFASSSKQRRAGQVLARPKNKRTVKCLETSSDYTDANENSEDIAEPPCEQICPTSDEAWLRYWQQHGEGLLWQSWLEKHPENSSSTVQDFSHSQTDWDDHFQQIYKYYWEQFQYWAAQGWTLDESYSTTAASEENQNCQGETEALESETKDRSKADASSVTHLISSLSLLTEEVDMDDGRQPLVCSCADEPCDGGNRKRPSSRAACTDASDGNMKQTSGSAPMNNGSGRPTKSRSNDEDQSDDDDDDEPPGNRRAKVKRAHELDAEEIAEVPVEEAWDALGLRRGSQPKFESVVKLKAGQGSYTGRRREDRKPPEQRRKAACKINKHIFFTDDGPQTTKPKISKALQKVQSFLQQVQTDADSEDVATTSMPEALIGPFDKAELASLPTRKEDEEEASNDTDEEDCETTPQPSRICEEQQHIIPDLEEIHEDEADPRGELQPLDVPDFLLPDAHEDTKKESVQAFKKNKRKKRKRRKDVEMPPEIAAEPELAKYWAQRYRLFSRFDEGIKLDHEGWFSVTPEKIAEHIALRVQDCFNTELIIDAFCGVGGNAIQFALTGKRVIGIDIDPVRLALAQHNAAVYGVEQRIDFLQGDFLQLAPHLRGDMVFLSPPWGGPEYLTADVFNIQTMMTPDGFEIFRLSKMISDNIVYFLPRNADMEQIASLAGPGGKVEVEQNFLNNKLKTITAYFGNLIK
InterPro domains: - IPR029063: S-adenosyl-L-methionine-dependent methyltransferase superfamily (homologous_superfamily) [587-800]
- IPR019012: RNA cap guanine-N2 methyltransferase (family) [645-797]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0044237 (cellular metabolic process), GO:0032259 (methylation), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0043414 (macromolecule methylation), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0001510 (RNA methylation), GO:0006996 (organelle organization), GO:0010467 (gene expression), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0042254 (ribosome biogenesis), GO:0016070 (RNA metabolic process), GO:0022618 (ribonucleoprotein complex assembly), GO:0031167 (rRNA methylation), GO:0006396 (RNA processing), GO:0071826 (ribonucleoprotein complex subunit organization), GO:0009451 (RNA modification), GO:0042255 (ribosome assembly), GO:0034470 (ncRNA processing), GO:0140694 (non-membrane-bounded organelle assembly), GO:0006364 (rRNA processing), GO:0034660 (ncRNA metabolic process), GO:0016072 (rRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)