Protein: Rho GTPase-activating protein 26 (GTPase regulator associated with focal adhesion kinase) (GRAF1) (Oligophrenin-1-like protein) (Rho-type GTPase-activating protein 26) (Q9UNA1)
Organism: Homo sapiens (Human)
Function: GTPase-activating protein for RHOA and CDC42.
Subcellular location: [Isoform 2]: Endosome membrane {ECO:0000269|PubMed:32344433}. Note=Colocalized with RAB8A, RAB8B and RAB10 on endosomal tubules. {ECO:0000269|PubMed:32344433}.; SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:27512058, ECO:0000269|PubMed:38081847}. Cell junction, focal adhesion {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Note=Colocalizes with actin stress fibers and cortical actin structures. {ECO:0000250}.
Sequence: MGLPALEFSDCCLDSPHFRETLKSHEAELDKTNKFIKELIKDGKSLISALKNLSSAKRKFADSLNEFKFQCIGDAETDDEMCIARSLQEFATVLRNLEDERIRMIENASEVLITPLEKFRKEQIGAAKEAKKKYDKETEKYCGILEKHLNLSSKKKESQLQEADSQVDLVRQHFYEVSLEYVFKVQEVQERKMFEFVEPLLAFLQGLFTFYHHGYELAKDFGDFKTQLTISIQNTRNRFEGTRSEVESLMKKMKENPLEHKTISPYTMEGYLYVQEKRHFGTSWVKHYCTYQRDSKQITMVPFDQKSGGKGGEDESVILKSCTRRKTDSIEKRFCFDVEAVDRPGVITMQALSEEDRRLWMEAMDGREPVYNSNKDSQSEGTAQLDSIGFSIIRKCIHAVETRGINEQGLYRIVGVNSRVQKLLSVLMDPKTASETETDICAEWEIKTITSALKTYLRMLPGPLMMYQFQRSFIKAAKLENQESRVSEIHSLVHRLPEKNRQMLQLLMNHLANVANNHKQNLMTVANLGVVFGPTLLRPQEETVAAIMDIKFQNIVIEILIENHEKIFNTVPDMPLTNAQLHLSRKKSSDSKPPSCSERPLTLFHTVQSTEKQEQRNSIINSSLESVSSNPNSILNSSSSLQPNMNSSDPDLAVVKPTRPNSLPPNPSPTSPLSPSWPMFSAPSSPMPTSSTSSDSSPVRSVAGFVWFSVAAVVLSLARSSLHAVFSLLVNFVPCHPNLHLLFDRPEEAVHEDSSTPFRKAKALYACKAEHDSELSFTAGTVFDNVHPSQEPGWLEGTLNGKTGLIPENYVEFL
InterPro domains: - IPR001849: Pleckstrin homology domain (domain) [265-371]
- IPR027267: AH/BAR domain superfamily (homologous_superfamily) [3-289]
- IPR036028: SH3-like domain superfamily (homologous_superfamily) [664-813]
- IPR047234: GRAF family (family) [1-806]
- IPR001452: SH3 domain (domain) [756-814]
- IPR047225: GRAF, PH domain (domain) [267-371]
- IPR000198: Rho GTPase-activating protein domain (domain) [383-568]
- IPR035483: GRAF, BAR domain (domain) [19-225]
- IPR004148: BAR domain (domain) [6-248]
- IPR008936: Rho GTPase activation protein (homologous_superfamily) [383-583]
- IPR035481: GRAF, SH3 domain (domain) [759-814]
- IPR011993: PH-like domain superfamily (homologous_superfamily) [259-378]
Interaction partners: - Serine/threonine-protein kinase N3
- Transforming protein RhoA
- Rho GTPase-activating protein 10
- Cell division control protein 42 homolog
- Delta and Notch-like epidermal growth factor-related receptor
- Cdc42 effector protein 2
- Amphiphysin
- C2 domain-containing protein 2
- Claudin-18
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0016043 (cellular component organization), GO:0044085 (cellular component biogenesis), GO:0006996 (organelle organization), GO:0030030 (cell projection organization), GO:0022607 (cellular component assembly), GO:0044782 (cilium organization), GO:0070925 (organelle assembly), GO:0030031 (cell projection assembly), GO:0120036 (plasma membrane bounded cell projection organization), GO:0060271 (cilium assembly), GO:0120031 (plasma membrane bounded cell projection assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)