Protein: Fumarate reductase iron-sulfur subunit (EC 1.3.5.1) (Quinol-fumarate reductase iron-sulfur subunit) (QFR iron-sulfur subunit) (P0AC47)
Organism: Escherichia coli (strain K12)
Function: Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; fumarate reductase is used during anaerobic growth, and succinate dehydrogenase is used during aerobic growth. The QFR enzyme complex binds 2 quinones in or near the membrane; 1 near the [3Fe-4S] cluster (QP is proximal to the [3Fe-4S] cluster, on the cytoplasmic side of the membrane) while QD (the distal cluster) is on the other side of the membrane. It is not clear if both of the quinol-binding sites are functionally relevant.
Subcellular location: Cell inner membrane {ECO:0000305|PubMed:10373108}; Peripheral membrane protein {ECO:0000305|PubMed:10373108}; Cytoplasmic side {ECO:0000305|PubMed:10373108}.
Sequence: MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYIKDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERDLVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIATLKPR
InterPro domains: - IPR036010: 2Fe-2S ferredoxin-like superfamily (homologous_superfamily) [4-106]
- IPR001041: 2Fe-2S ferredoxin-type iron-sulfur binding domain (domain) [16-97]
- IPR017896: 4Fe-4S ferredoxin-type, iron-sulphur binding domain (domain) [140-216]
- IPR009051: Alpha-helical ferredoxin (homologous_superfamily) [107-244]
- IPR006058: 2Fe-2S ferredoxin, iron-sulphur binding site (binding_site) [58-66]
- IPR004489: Succinate dehydrogenase/fumarate reductase iron-sulphur protein (family) [11-229]
- IPR017900: 4Fe-4S ferredoxin, iron-sulphur binding, conserved site (conserved_site) [149-160]
- IPR012675: Beta-grasp domain superfamily (homologous_superfamily) [3-106]
- IPR025192: Succinate dehydogenase/fumarate reductase N-terminal (domain) [8-111]
Interaction partners: - Fumarate reductase subunit C
- Fumarate reductase flavoprotein subunit
- Succinate dehydrogenase flavoprotein subunit
- Fumarate reductase subunit D
- Succinate dehydrogenase cytochrome b556 subunit
- NADH-quinone oxidoreductase subunit C/D
- Succinate dehydrogenase hydrophobic membrane anchor subunit
- Succinate--CoA ligase subunit beta
- Succinate--CoA ligase subunit alpha
- Probable pyruvate-flavodoxin oxidoreductase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0051540 (metal cluster binding), GO:0005515 (protein binding), GO:0016491 (oxidoreductase activity), GO:0009055 (electron transfer activity), GO:0051536 (iron-sulfur cluster binding), GO:0051537 (2 iron, 2 sulfur cluster binding), GO:0051539 (4 iron, 4 sulfur cluster binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006091 (generation of precursor metabolites and energy), GO:0015980 (energy derivation by oxidation of organic compounds), GO:0045333 (cellular respiration), GO:0009060 (aerobic respiration)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0016020 (membrane), GO:1902494 (catalytic complex), GO:0071944 (cell periphery), GO:0070469 (respirasome), GO:0098796 (membrane protein complex), GO:0098797 (plasma membrane protein complex), GO:0098803 (respiratory chain complex), GO:0070470 (plasma membrane respirasome), GO:1990204 (oxidoreductase complex), GO:0045273 (respiratory chain complex II), GO:0005886 (plasma membrane)