Protein: Lysosomal proton-coupled steroid conjugate and bile acid symporter SLC46A3 (Q9DC26)
Organism: Mus musculus (Mouse)
Function: Lysosomal proton-coupled steroid conjugate and bile acid transporter. Preferentially recognizes lipophilic steroid conjugates or bile acis as endogenous substrates and seems to mediate escape from lysosomes to the cytoplasm (By similarity). Modulates hepatic cytosolic copper homeostasis, maybe acting as a lysosomal copper transporter and sequestering copper ions in the lysosome. Delivers pathogen-associated molecular patterns to cytosolic pattern recognition receptors as part of the innate immune response to microbes. Selectively transports bacterial muramyl dipeptide (MDP) into the cytosol for recognition by NOD2, triggering inflammatory responses. Likely acts as a redundant importer of cyclic GMP-AMP dinucleotides (cGAMPs) in monocyte and macrophage cell lineages. The transport mechanism, its electrogenicity and stoichiometry remain to be elucidated (Probable)
Subcellular location: Lysosome membrane; Multi-pass membrane protein
Sequence: MKISFIEPAILLNAFAMTLTIPLTAQYVYRRIWEETGNYTFASNSNGSECDQNKSSSIFAFREEVQKKASLFNLQVEMSALIPGLVSTFMLLASSDNHGRKLPMVLSSLGSLGTNTWLCMMSYFDLPLQLLIASTFIGALFGNYTTFWGACFAYIVDQQKEYKHRIIRIAILDFMLGVVTGLTGLSSGYFIRELGFVWSYFITAMVLIVNLAYILFFLNDPIKESSSQIVTMSCIESLKDLFYRTYMLFKNGSSKRQALLCLLIFTLVIYFFVIIGISPIFTLYELGPPLCWNEVYIGYGSALGSVSFLSSFLGIWLFSYCLKDIHIAYIGIFTTMVGMTLAAFTRTTLMMFLVRIPFIFTIMPLSVLRSMLSKVVHSTEQGALFACIAFLETLAGVTSTSAYSGIYSATVAWYPGFIFLLSAGLLVLPAISLCCVKSIGWEEGSYTLLVHEEPSEHTSD
InterPro domains: - IPR036259: MFS transporter superfamily (homologous_superfamily) [9-435]
- IPR011701: Major facilitator superfamily (family) [81-398]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0055085 (transmembrane transport), GO:0051234 (establishment of localization), GO:1903825 (organic acid transmembrane transport), GO:0098739 (import across plasma membrane), GO:0006810 (transport), GO:0035461 (vitamin transmembrane transport), GO:0098657 (import into cell), GO:0071705 (nitrogen compound transport), GO:0051180 (vitamin transport), GO:0071702 (organic substance transport), GO:1905039 (carboxylic acid transmembrane transport), GO:0006811 (monoatomic ion transport), GO:0042886 (amide transport), GO:0006812 (monoatomic cation transport), GO:0015711 (organic anion transport), GO:0015886 (heme transport), GO:0015884 (folic acid transport), GO:0072337 (modified amino acid transport), GO:0015849 (organic acid transport), GO:0030001 (metal ion transport), GO:0046942 (carboxylic acid transport), GO:0006835 (dicarboxylic acid transport), GO:0000041 (transition metal ion transport), GO:0006826 (iron ion transport), GO:1901678 (iron coordination entity transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0005773 (vacuole), GO:0031090 (organelle membrane), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000323 (lytic vacuole), GO:0043231 (intracellular membrane-bounded organelle), GO:0005774 (vacuolar membrane), GO:0098588 (bounding membrane of organelle), GO:0005764 (lysosome), GO:0098852 (lytic vacuole membrane), GO:0005765 (lysosomal membrane)