Protein: Acyl-CoA dehydrogenase FadE29 (ACAD) (EC 1.3.99.-) (3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA dehydrogenase beta subunit) (3-oxo-4-pregnene-20-carboxyl-CoA dehydrogenase beta subunit) (P71858)
Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Function: Involved in the third cycle of side chain dehydrogenation in the beta-oxidation of cholesterol catabolism. Contributes partly to the virulence by increasing the efficiency of beta-oxidation. Catalyzes the dehydrogenation of 2'-propanoyl-CoA ester side chains of 3-oxo-4-pregnene-20-carboxyl-CoA (3-OPC-CoA) to yield 3-oxo-4,17-pregnadiene-20-carboxyl-CoA (3-OPDC-CoA). Also able to dehydrogenate steroyl-CoA such as 3-oxo-chol-4-en-24-oyl-CoA (3-OCO-CoA), 1beta-(2'-propanoyl-CoA)-3a-alpha-H- 7a-beta-methylhexahydro-4-indanone (indanone-CoA ester), hexahydroindanone and pregenenone.
Sequence: MFIDLTPEQRQLQAEIRQYFSNLISPDERTEMEKDRHGPAYRAVIRRMGRDGRLGVGWPKEFGGLGFGPIEQQIFVNEAHRADVPLPAVTLQTVGPTLQAHGSELQKKKFLPAILAGEAHFAIGYTEPEAGTDLASLRTTAVRDGDHYIVNGQKVFTTGAHDADYIWLACRTDPNAAKHKGISILIVDTKDPGYSWTPIILADGAHHTNATYYNDVRVPVDMLVGKENDGWRLITTQLNNERVMLGPAGRFASIYDRVHAWASVPGGNGVTPIDHDDVKRALGEIRAIWRINELLNWQVASAGEDINMADAAATKVFGTERVQRAGRLAEEIVGKYGNPAEPDTAELLRWLDAQTKRNLVITFGGGVNEVMREMIAASGLKVPRVPR
InterPro domains: - IPR037069: Acyl-CoA dehydrogenase/oxidase, N-terminal domain superfamily (homologous_superfamily) [2-117]
- IPR013786: Acyl-CoA dehydrogenase/oxidase, N-terminal (domain) [6-118]
- IPR036250: Acyl-CoA dehydrogenase-like, C-terminal (homologous_superfamily) [228-378]
- IPR006091: Acyl-CoA oxidase/dehydrogenase, middle domain (domain) [122-207]
- IPR052161: Mycobacterial Acyl-CoA Dehydrogenase (family) [1-385]
- IPR009100: Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain superfamily (homologous_superfamily) [4-241]
- IPR046373: Acyl-CoA oxidase/dehydrogenase, middle domain superfamily (homologous_superfamily) [121-231]
- IPR009075: Acyl-CoA dehydrogenase/oxidase, C-terminal (domain) [228-378]
Interaction partners: - Acyl-CoA dehydrogenase FadE28
- 3-oxo-4,17-pregnadiene-20-carboxyl-CoA hydratase beta subunit
- 17-hydroxy-3-oxo-4-pregnene-20-carboxyl-CoA lyase
- Steroid 3-ketoacyl-CoA thiolase
- 3-oxo-4,17-pregnadiene-20-carboxyl-CoA hydratase alpha subunit
- Steroid C26-monooxygenase-3-oxocholest-4-en-26-oate forming])
- Possible lipid transfer protein or keto acyl-CoA thiolase Ltp4
- Probable lipid carrier protein or keto acyl-CoA thiolase Ltp3
- (7aS)-7a-methyl-1,5-dioxo-2,3,5,6,7,7a-hexahydro-1H-indene-carboxyl-CoA hydrolase (HIEC-CoA hydrolase)
- Probable acyl-CoA dehydrogenase FadE32
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016627 (oxidoreductase activity, acting on the CH-CH group of donors), GO:0052890 (oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor), GO:0003995 (acyl-CoA dehydrogenase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0120252 (hydrocarbon metabolic process), GO:0006629 (lipid metabolic process), GO:1901575 (organic substance catabolic process), GO:0044248 (cellular catabolic process), GO:0006720 (isoprenoid metabolic process), GO:0042214 (terpene metabolic process), GO:0044242 (cellular lipid catabolic process), GO:0016042 (lipid catabolic process), GO:0120253 (hydrocarbon catabolic process), GO:0046247 (terpene catabolic process), GO:0008300 (isoprenoid catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0005886 (plasma membrane), GO:0009274 (peptidoglycan-based cell wall)