Protein: Sodium/hydrogen exchanger 5 (Na(+)/H(+) exchanger 5) (NHE-5) (Solute carrier family 9 member 5) (Q9Z0X2)
Organism: Rattus norvegicus (Rat)
Function: Plasma membrane Na(+)/H(+) antiporter. Mediates the electroneutral exchange of intracellular H(+) ions for extracellular Na(+) in 1:1 stoichiometry. Responsible for regulating intracellular pH homeostasis, in particular in neural tissues. Acts as a negative regulator of dendritic spine growth. Plays a role in postsynaptic remodeling and signaling. Can also contribute to organellar pH regulation, with consequences for receptor tyrosine kinase trafficking (By similarity).
Subcellular location: Cell membrane {ECO:0000250|UniProtKB:Q14940}; Multi-pass membrane protein {ECO:0000255}. Recycling endosome membrane {ECO:0000250|UniProtKB:Q14940}; Multi-pass membrane protein {ECO:0000255}. Cell projection, dendritic spine membrane {ECO:0000250|UniProtKB:Q14940}; Multi-pass membrane protein {ECO:0000255}. Synaptic cell membrane {ECO:0000250|UniProtKB:Q14940}; Multi-pass membrane protein. Cell junction, focal adhesion {ECO:0000250|UniProtKB:Q14940}. Note=Cycles between recycling endosome and plasma membrane in response to diverse stimuli. Its internalization is clathrin- and beta-arrestin dependent and its plasma membrane insertion from the recycling endosomes requires phosphoinositide 3-kinase (PIK3CA) and SCAMP2. {ECO:0000250|UniProtKB:Q14940}.
Sequence: MLRVALLLLPGLPLAGVGATEEPTQEPGPLGEPPGLALFRWQWHEVEAPYLVALWILVASLAKIVFHLSRKVTSLVPESCLLILLGLALGGIVLAVAKKAEYQLEPGTFFLFLLPPIVLDSGYFMPSRLFFDNLGAILTYAVVGTLWNAFTTGVALWGLQQAGLVAPRVQAGLLDFLLFGSLISAVDPVAVLAVFEEVHVNETLFIIVFGESLLNDAVTVVLYKVCNSFVEMGSANVQATDYLKGVASLFVVSLGGAAVGLVFAFLLALTTRFTKRVRIIEPLLVFLLAYAAYLTAEMASLSAILAVTMCGLGCKKYVEANISHKSRTAVKYTMKTLASSAETVIFMLLGISAVDSSKWAWDSGLVLGTLFFILFFRALGVVLQTWVLNQFRLVPLDKIDQVVMSYGGLRGAVAFALVILLDRTKVPAKDYFVATTIVVVFFTVIVQGLTIKPLVKWLRVKRSDYHKPTLNQELHEHTFDHILAAVEDVVGHHGYHYWRDRWEQFDKKYLSQLLMRRSAYRIRDQIWDVYYRLNIRDAISFVDQGGHVLSSAGLTLPSMPSRNSVAETSVTNLLRESGSGACLDLQVIDTVRSGRDREDAVMHHLLCGGLYKPRRRYKASCSRHFISEDAQERQDKEVFQQNMKRRLESFKSTKHNICFTKSKPRPRKTGHKKKDGVANPEATNGKPPRDLGFQDTAAVILTVESEEEEESESSETEKEDDEGIIFVARATSEVLQEGKVSGSLEVCPSPRIIPPSPTCAEKELPWKSGQGDLAVYVSSETTKIVPVDMQTGWNQSISSLESLASPPCTQPPTLTRLPPHPLVPEEPQVPLDLSSDPRSSFAFPPSLAKAGRSRSESSADIPQQQELQPLMGHKDHTHLSPGPANSHWCIQFNRGGRL
InterPro domains: - IPR018410: Na+/H+ exchanger, isoforms 3/5 (family) [47-734]
- IPR006153: Cation/H+ exchanger, transmembrane domain (domain) [56-457]
- IPR004709: Na+/H+ exchanger (family) [42-631]
- IPR018422: Cation/H+ exchanger, CPA1 family (family) [26-623]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity), GO:0005515 (protein binding), GO:1901702 (salt transmembrane transporter activity), GO:0015075 (monoatomic ion transmembrane transporter activity), GO:0015318 (inorganic molecular entity transmembrane transporter activity), GO:0022804 (active transmembrane transporter activity), GO:0022890 (inorganic cation transmembrane transporter activity), GO:0022853 (active monoatomic ion transmembrane transporter activity), GO:0008324 (monoatomic cation transmembrane transporter activity), GO:0015081 (sodium ion transmembrane transporter activity), GO:0015291 (secondary active transmembrane transporter activity), GO:0015297 (antiporter activity), GO:0015078 (proton transmembrane transporter activity), GO:0046873 (metal ion transmembrane transporter activity), GO:0140828 (metal cation:monoatomic cation antiporter activity), GO:0015385 (sodium:proton antiporter activity), GO:0051139 (metal cation:proton antiporter activity)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0009628 (response to abiotic stimulus), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0051240 (positive regulation of multicellular organismal process), GO:0051239 (regulation of multicellular organismal process), GO:0045927 (positive regulation of growth), GO:0050793 (regulation of developmental process), GO:0009719 (response to endogenous stimulus), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0051716 (cellular response to stimulus), GO:0051094 (positive regulation of developmental process), GO:0048523 (negative regulation of cellular process), GO:0040008 (regulation of growth), GO:0048522 (positive regulation of cellular process), GO:0048639 (positive regulation of developmental growth), GO:0045597 (positive regulation of cell differentiation), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0009314 (response to radiation), GO:0001558 (regulation of cell growth), GO:0071495 (cellular response to endogenous stimulus), GO:0060255 (regulation of macromolecule metabolic process), GO:1901700 (response to oxygen-containing compound), GO:0030307 (positive regulation of cell growth), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:0009891 (positive regulation of biosynthetic process), GO:0009991 (response to extracellular stimulus), GO:0010035 (response to inorganic substance), GO:2000026 (regulation of multicellular organismal development), GO:0007584 (response to nutrient), GO:1901698 (response to nitrogen compound), GO:0031325 (positive regulation of cellular metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0045595 (regulation of cell differentiation), GO:0031323 (regulation of cellular metabolic process), GO:0031668 (cellular response to extracellular stimulus), GO:0080090 (regulation of primary metabolic process), GO:0051128 (regulation of cellular component organization), GO:0051962 (positive regulation of nervous system development), GO:0071496 (cellular response to external stimulus), GO:0010038 (response to metal ion), GO:0071417 (cellular response to organonitrogen compound), GO:0046683 (response to organophosphorus), GO:0009416 (response to light stimulus), GO:0034097 (response to cytokine), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0071310 (cellular response to organic substance), GO:0060284 (regulation of cell development), GO:0010720 (positive regulation of cell development), GO:1901699 (cellular response to nitrogen compound), GO:0050769 (positive regulation of neurogenesis), GO:0051960 (regulation of nervous system development), GO:0010468 (regulation of gene expression), GO:0033280 (response to vitamin D), GO:0061387 (regulation of extent of cell growth), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051591 (response to cAMP), GO:0051254 (positive regulation of RNA metabolic process), GO:0010243 (response to organonitrogen compound), GO:0033993 (response to lipid), GO:0031667 (response to nutrient levels), GO:0031326 (regulation of cellular biosynthetic process), GO:0031670 (cellular response to nutrient), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0014070 (response to organic cyclic compound), GO:0051252 (regulation of RNA metabolic process), GO:1901701 (cellular response to oxygen-containing compound), GO:0031669 (cellular response to nutrient levels), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0045471 (response to ethanol), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0071407 (cellular response to organic cyclic compound), GO:0071345 (cellular response to cytokine stimulus), GO:0071396 (cellular response to lipid), GO:0002526 (acute inflammatory response), GO:0010043 (response to zinc ion), GO:0034612 (response to tumor necrosis factor), GO:1902680 (positive regulation of RNA biosynthetic process), GO:0050767 (regulation of neurogenesis), GO:0014074 (response to purine-containing compound), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045893 (positive regulation of DNA-templated transcription), GO:0051384 (response to glucocorticoid), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0071356 (cellular response to tumor necrosis factor), GO:0045944 (positive regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0045178 (basal part of cell), GO:0043226 (organelle), GO:0016020 (membrane), GO:0009986 (cell surface), GO:0048471 (perinuclear region of cytoplasm), GO:0071944 (cell periphery), GO:0030054 (cell junction), GO:0045177 (apical part of cell), GO:0030315 (T-tubule), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0016324 (apical plasma membrane), GO:0009925 (basal plasma membrane), GO:0005739 (mitochondrion), GO:0098590 (plasma membrane region), GO:0005783 (endoplasmic reticulum), GO:0098857 (membrane microdomain), GO:0070161 (anchoring junction), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0045121 (membrane raft), GO:0043231 (intracellular membrane-bounded organelle), GO:0042383 (sarcolemma), GO:0016323 (basolateral plasma membrane), GO:0005911 (cell-cell junction), GO:0044291 (cell-cell contact zone), GO:0014704 (intercalated disc)