Protein: DNA-directed RNA polymerase I subunit RPA43 (DNA-directed RNA polymerase I subunit F) (Twist neighbor protein) (Q78WZ7)
Organism: Mus musculus (Mouse)
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through its association with RRN3/TIF-IA may be involved in recruitment of Pol I to rDNA promoters.
Subcellular location: Nucleus, nucleolus {ECO:0000250|UniProtKB:Q3B726}.
Sequence: MAAGSVESQRSQAASERPVAGQAGVLPCLELPSYAAACALVGSRYSCLVAAPHRRHIALSPRYLSRKRTGIREQLDAELLRYSESLLGVPIAYDNIRVVGELGDIYDDQGHIHLNIEADFVIFCPEPGQTLMGTVNKVSSSHIGCLVHGCFNASIPKPEQMSYEEWQTLEIHVGDELEFDVFRLDSDSAGVFCIRGKLSTTSLQLKHSAVSEDVAETVVEEVVEKTPKKKKKKKDKDTDTCGTVDSVTEVADVTDVTPQEETDIPCSDNVNDFFEEEPKKKKKKKKRHQEDQDPIFQASDSSGYQSDHNKKKKKRKHSEEANFESPKKRQ
InterPro domains: - IPR036898: RNA polymerase Rpb7-like, N-terminal domain superfamily (homologous_superfamily) [38-128]
- IPR041178: RPA43, OB domain (domain) [125-242]
- IPR005576: RNA polymerase Rpb7-like , N-terminal (domain) [55-110]
- IPR041901: Rpa43, N-terminal ribonucleoprotein (RNP) domain (domain) [43-126]
- IPR045113: RNA polymerase subunit Rpb7-like (family) [49-277]
Interaction partners: - DNA-directed RNA polymerase I subunit RPA2
- DNA-directed RNA polymerases I, II, and III subunit RPABC1
- DNA-directed RNA polymerases I and III subunit RPAC1
- DNA-directed RNA polymerase I subunit RPA34
- DNA-directed RNA polymerase I subunit RPA49
- DNA-directed RNA polymerases I and III subunit RPAC2
- DNA-directed RNA polymerase I subunit RPA1
- DNA-directed RNA polymerases I, II, and III subunit RPABC2
- DNA-directed RNA polymerase I subunit RPA12
- DNA-directed RNA polymerases I, II, and III subunit RPABC4
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0016740 (transferase activity), GO:0140098 (catalytic activity, acting on RNA), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0097747 (RNA polymerase activity), GO:0016779 (nucleotidyltransferase activity), GO:0034062 (5'-3' RNA polymerase activity), GO:0003899 (DNA-directed 5'-3' RNA polymerase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0010467 (gene expression), GO:0018130 (heterocycle biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0016070 (RNA metabolic process), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006351 (DNA-templated transcription), GO:0006366 (transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0043229 (intracellular organelle), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus)