Protein: Poly(A)-specific ribonuclease PARN-like (EC 3.1.13.4) (Polyadenylate-specific ribonuclease-like protein) (Q8W4C3)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs (By similarity).
Subcellular location: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
Sequence: MQRRFLSSISATAGNTKTLNQGRWSVKQVKKSNFHVTLDEIRTSIDSSDFIALSLQNTGSYAAAWHRVSAIDTPQTSYLKAKYAAERYQILQFALCPFSLQGSKLTVHPYNFHLFPRDELKCGMPSYSFSCQASRLTAMAREGFDFNICIYEGISYLSRAQESASKFLSENPILADSVTVSSSPATVADTVFVGRIRSRVKNWRQSCIDSGSKTGDDDLVSSLRRLVLGSEQYGSRLCLTIDVCSERQVQLILEMLTEFSDDVVPLLVASKSRGTQAVRTVFMSSKEDKDLFKRELKDLEKEENRRVRGFREVVDFISSSQKPVVSQNYLSDFTSIHAKFLGPLPSNVDDFSSSLSSAFPNVVDLSQFMKEISPLSNISNLPAAMSSLNRFFAPVDVEVANQGCPVKLDEGHQSHGQNAVMISQLFAKLCTIQKSDLSTIQSNEDFQALASDEHANSVTSCSKNAGDENVKVWSKNSRRVSSENLVFIWGLGKKMTAAKLKNVLQKSHPVFAREFDVKYIDRSSAILVFWESGPSETFLSAVNNEEQLDGSLREMVAEGLRGAGYETYKRACRLGFWEADLAESLDKALESSDTDPDSDTKPSEIDWSNELAINFDEL
InterPro domains: - IPR051181: CAF1 family poly(A)-specific ribonucleases (family) [28-569]
- IPR006941: Ribonuclease CAF1 (family) [29-402]
- IPR012337: Ribonuclease H-like superfamily (homologous_superfamily) [19-439]
- IPR036397: Ribonuclease H superfamily (homologous_superfamily) [28-467]
Interaction partners: - Poly
- Uncharacterized CRM domain-containing protein At3g25440
- Exosome complex component RRP4 homolog
- mRNA-decapping enzyme subunit 2
- Pentatricopeptide repeat-containing protein At2g33680
- Pentatricopeptide repeat-containing protein At1g32415
- AT3g56680/T8M16_10
- Polyribonucleotide nucleotidyltransferase 2, mitochondrial
- Putative defensin-like protein 148
- Tetratricopeptide repeat protein SKI3
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0016787 (hydrolase activity), GO:0140098 (catalytic activity, acting on RNA), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0004518 (nuclease activity), GO:0004540 (ribonuclease activity), GO:0004532 (exoribonuclease activity), GO:0004527 (exonuclease activity), GO:0008408 (3'-5' exonuclease activity), GO:0016896 (RNA exonuclease activity, producing 5'-phosphomonoesters), GO:0016796 (exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters), GO:0000175 (3'-5'-RNA exonuclease activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0009628 (response to abiotic stimulus), GO:0006950 (response to stress), GO:0042221 (response to chemical), GO:0009719 (response to endogenous stimulus), GO:0006970 (response to osmotic stress), GO:1901700 (response to oxygen-containing compound), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:0097305 (response to alcohol), GO:0009651 (response to salt stress), GO:0009737 (response to abscisic acid), GO:0014070 (response to organic cyclic compound), GO:0033993 (response to lipid), GO:0009751 (response to salicylic acid)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)