Protein: Ras-like GTP-binding protein RHO1 (P31021)
Organism: Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM)
Function: Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. Involved in actin cytoskeleton remodeling. Regulates phagocytosis by modulating actin cytoskeleton dynamics through the recruitment of formin1 and profilin1 to the phagocytosis nucleation site
Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, phagocytic cup. Cytoplasmic vesicle, phagosome. Note=Co-localizes in its GTP-bound active form with F-actin at the nucleation sites of phagocytic cups where it remains until the closure of phagocytic cups. Localizes to phagosomes after their separation from the cell membrane
Sequence: MLAFSDMNTGAGKIENGKKALKIVVVGDGAVGKTCLLLAFSKGEIPTAYVPTVFENFSHVMKYKNEEFILHLWDTAGQEEYDRLRPLSYADSDVVLLCFAVNNRTSFDNISTKWEPEIKHYIDTAKTVLVGLKVDLRKDGSDDVTKQEGDDLCQKLGCVAYIEASSVAKIGLNEVFEKSVDCIFSNKPVPKASVTTQAKSQESTQQKKKSKCLLQ
InterPro domains: - IPR001806: Small GTPase (family) [16-214]
- IPR005225: Small GTP-binding domain (domain) [19-178]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [12-214]
- IPR003578: Small GTPase Rho (family) [20-213]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0097367 (carbohydrate derivative binding), GO:0005515 (protein binding), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0032553 (ribonucleotide binding), GO:0035639 (purine ribonucleoside triphosphate binding), GO:0032555 (purine ribonucleotide binding), GO:0017076 (purine nucleotide binding), GO:0005525 (GTP binding), GO:0019001 (guanyl nucleotide binding), GO:0032561 (guanyl ribonucleotide binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0051179 (localization), GO:0051234 (establishment of localization), GO:0051641 (cellular localization), GO:0071840 (cellular component organization or biogenesis), GO:0140352 (export from cell), GO:0033036 (macromolecule localization), GO:0070727 (cellular macromolecule localization), GO:0032940 (secretion by cell), GO:0016043 (cellular component organization), GO:0051668 (localization within membrane), GO:0045184 (establishment of protein localization), GO:0006810 (transport), GO:0035592 (establishment of protein localization to extracellular region), GO:0071702 (organic substance transport), GO:0046903 (secretion), GO:0016192 (vesicle-mediated transport), GO:0008104 (protein localization), GO:0071705 (nitrogen compound transport), GO:0009306 (protein secretion), GO:0006996 (organelle organization), GO:0010256 (endomembrane system organization), GO:0015031 (protein transport), GO:0007030 (Golgi organization), GO:0071692 (protein localization to extracellular region)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0001891 (phagocytic cup), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0071944 (cell periphery), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0097708 (intracellular vesicle)