Protein: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 (EC 2.1.1.14) (Cobalamin-independent methionine synthase 2) (AtMS2) (Q9SRV5)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:15024005}.
Sequence: MASHIVGYPRMGPKRELKFALESFWDGKSSADDLQKVSADLRSDIWKQMSAAGIKYIPSNTFSHYDQVLDTTAMLGAVPSRYGFTSGEIGLDVYFSMARGNASVPAMEMTKWFDTNYHYIVPELGPEVKFSYASHKAVNEYKEAKALGVETVPVLVGPVSYLLLSKLAKGVDKSFDLLSLLPKILPVYKEVIAELKAAGASWIQLDEPLFVMDLEGHKLQAFSGAYAELESTLSGLNVLVETYFADIPAEAYKTLTSLKGVTAFGFDLVRGTKTIDLIKSGFPQGKYLFAGVVDGRNIWANDLAASLITLQSLEGVVGKDKLVVSTSCSLLHTAVDLINETKLDAEIKSWLAFAAQKVVEVDALAKALAGQTNESFFTANADALSSRRSSPRVTNESVQKAAAALKGSDHRRTTEVSARLDAQQKKLNLPILPTTTIGSFPQTVELRRVRREYKAKKISEEDYVKAIKEEIKKVVDIQEDLDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPVIYGDVSRPKPMTVFWSSTAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKGGIGVIQIDEAALREGLPLRKAEHSFYLDWAVHSFRITNCGVQDSTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTDEIADRINKMLAVLEQNILWVNPDCGLKTRKYTEVKPALKAMVDAAKLIRSQLGSAK
InterPro domains: - IPR002629: Cobalamin-independent methionine synthase MetE, C-terminal/archaeal (domain) [432-756]
- IPR013215: Cobalamin-independent methionine synthase MetE, N-terminal (domain) [3-316]
- IPR038071: UROD/MetE-like superfamily (homologous_superfamily) [3-759]
- IPR006276: Cobalamin-independent methionine synthase (family) [1-765]
Interaction partners: - Adenosylhomocysteinase 2
- Adenosylhomocysteinase 1
- Cystathionine beta-lyase, chloroplastic
- Homocysteine S-methyltransferase 1
- Homocysteine S-methyltransferase 2
- Homocysteine S-methyltransferase 3
- S-adenosylmethionine synthase 3
- S-adenosylmethionine synthase 2
- Cystathionine gamma-synthase 1, chloroplastic
- S-adenosylmethionine synthase 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0043169 (cation binding), GO:0046872 (metal ion binding), GO:0008168 (methyltransferase activity), GO:0003723 (RNA binding), GO:0003729 (mRNA binding), GO:0008172 (S-methyltransferase activity), GO:0046914 (transition metal ion binding), GO:0005507 (copper ion binding), GO:0008270 (zinc ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009058 (biosynthetic process), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0009892 (negative regulation of metabolic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0010035 (response to inorganic substance), GO:0044283 (small molecule biosynthetic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0006082 (organic acid metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0006790 (sulfur compound metabolic process), GO:0010038 (response to metal ion), GO:0000096 (sulfur amino acid metabolic process), GO:0051246 (regulation of protein metabolic process), GO:0008652 (amino acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0006325 (chromatin organization), GO:1901605 (alpha-amino acid metabolic process), GO:0031060 (regulation of histone methylation), GO:0010468 (regulation of gene expression), GO:1901566 (organonitrogen compound biosynthetic process), GO:0044272 (sulfur compound biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0031062 (positive regulation of histone methylation), GO:0051247 (positive regulation of protein metabolic process), GO:0010629 (negative regulation of gene expression), GO:1901607 (alpha-amino acid biosynthetic process), GO:0031399 (regulation of protein modification process), GO:0000097 (sulfur amino acid biosynthetic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0006555 (methionine metabolic process), GO:0051570 (regulation of histone H3-K9 methylation), GO:0019752 (carboxylic acid metabolic process), GO:0040029 (epigenetic regulation of gene expression), GO:0006338 (chromatin remodeling), GO:0045814 (negative regulation of gene expression, epigenetic), GO:0010043 (response to zinc ion), GO:0009066 (aspartate family amino acid metabolic process), GO:0031401 (positive regulation of protein modification process), GO:0031058 (positive regulation of histone modification), GO:0009086 (methionine biosynthetic process), GO:0031507 (heterochromatin formation), GO:0031056 (regulation of histone modification), GO:0009067 (aspartate family amino acid biosynthetic process), GO:0070828 (heterochromatin organization), GO:0140718 (facultative heterochromatin formation), GO:0071265 (L-methionine biosynthetic process), GO:0006346 (DNA methylation-dependent heterochromatin formation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0009532 (plastid stroma), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0030054 (cell junction), GO:0005829 (cytosol), GO:0055044 (symplast), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0005576 (extracellular region), GO:0005773 (vacuole), GO:0042579 (microbody), GO:0048046 (apoplast), GO:0043229 (intracellular organelle), GO:0009506 (plasmodesma), GO:0070161 (anchoring junction), GO:0043227 (membrane-bounded organelle), GO:0005886 (plasma membrane), GO:0009536 (plastid), GO:0009570 (chloroplast stroma), GO:0000325 (plant-type vacuole), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle), GO:0005777 (peroxisome), GO:0005911 (cell-cell junction)