Protein: Reverse rubrerythrin-2 (revRbr 2) (NADH peroxidase) (NPXase) (Npx) (EC 1.11.1.1) (Rubperoxin 2) (Rpr 2) (Q97D83)
Organism: Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / IAM 19013 / LMG 5710 / NBRC 13948 / NRRL B-527 / VKM B-1787 / 2291 / W)
Function: Functions as the terminal component of an NADH peroxidase (NADH:H(2)O(2) oxidoreductase) when using NADH:rubredoxin oxidoreductase (NROR) as the electron transport intermediary from NADH to revRbr 2. Plays an important role in the oxidative stress defense system in C.acetobutylicum, an obligate anaerobic bacterium. Also exhibits NADH oxidase (NADH:O(2) oxidoreductase) activity in vitro, which is 100-fold lesser than that of FprA1/2 using the same electron transfer components. Therefore, its predominant function is most likely as a scavenger of its preferred substrate, H(2)O(2).
Sequence: MKKFKCVVCGYIYTGEDAPEKCPVCGAGKDKFVEVKDEGEGWADEHKIGIAKGVDKEVLEGLRANFTGECTEVGMYLAMARQADREGYPEVAEAYKRIAFEEAEHASKFAELLGEVVVADTKTNLQMRVDAEKGACEGKKELATLAKKLNYDAIHDTVHEMCKDEARHGSAFRGLLNRYFK
InterPro domains: - IPR009040: Ferritin-like diiron domain (domain) [52-181]
- IPR003251: Rubrerythrin, diiron-binding domain (domain) [62-175]
- IPR012347: Ferritin-like (homologous_superfamily) [55-180]
- IPR024934: Rubredoxin-like domain (domain) [1-35]
- IPR045236: Reverse rubrerythrin, di-iron-binding domain (domain) [60-180]
- IPR052773: Anaerobic Bacterial Peroxidase-Related (family) [42-181]
- IPR009078: Ferritin-like superfamily (homologous_superfamily) [55-180]
- IPR048574: Rubrerythrin, rubredoxin-like domain (domain) [4-32]
Interaction partners: - Reverse rubrerythrin-1
- Rubredoxin
- Transcriptional regulator PerR
- Desulfoferrodoxin
- NADH-rubredoxin oxidoreductase
- Flavo-diiron protein FprA2
- Flavo-diiron protein FprA1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009628 (response to abiotic stimulus), GO:0042221 (response to chemical), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0009408 (response to heat), GO:0009266 (response to temperature stimulus), GO:0010035 (response to inorganic substance), GO:0104004 (cellular response to environmental stimulus), GO:0006979 (response to oxidative stress), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0071214 (cellular response to abiotic stimulus), GO:0070482 (response to oxygen levels), GO:0006970 (response to osmotic stress), GO:1901700 (response to oxygen-containing compound), GO:0009268 (response to pH), GO:0010033 (response to organic substance), GO:0009409 (response to cold), GO:0034599 (cellular response to oxidative stress), GO:0070417 (cellular response to cold), GO:0071453 (cellular response to oxygen levels), GO:0062197 (cellular response to chemical stress), GO:0071470 (cellular response to osmotic stress), GO:0034605 (cellular response to heat), GO:0009651 (response to salt stress), GO:0010446 (response to alkaline pH), GO:0042542 (response to hydrogen peroxide), GO:1901701 (cellular response to oxygen-containing compound), GO:0000302 (response to reactive oxygen species), GO:0071467 (cellular response to pH), GO:0071469 (cellular response to alkaline pH), GO:0034614 (cellular response to reactive oxygen species), GO:0071472 (cellular response to salt stress), GO:0070301 (cellular response to hydrogen peroxide)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)