Protein: Exosome complex component RRP46 (Ribosomal RNA-processing protein 46) (P53256)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and in RNA surveillance pathways, preventing translation of aberrant mRNAs. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. RRP46 is part of the hexameric ring of RNase PH domain-containing subunits proposed to form a central channel which threads RNA substrates for degradation.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus, nucleolus {ECO:0000269|PubMed:14562095}.
Sequence: MSVQAEIGILDHVDGSSEFVSQDTKVICSVTGPIEPKARQELPTQLALEIIVRPAKGVATTREKVLEDKLRAVLTPLITRHCYPRQLCQITCQILESGEDEAEFSLRELSCCINAAFLALVDAGIALNSMCASIPIAIIKDTSDIIVDPTAEQLKISLSVHTLALEFVNGGKVVKNVLLLDSNGDFNEDQLFSLLELGEQKCQELVTNIRRIIQDNISPRLVV
InterPro domains: - IPR036345: Exoribonuclease, PH domain 2 superfamily (homologous_superfamily) [127-217]
- IPR001247: Exoribonuclease, phosphorolytic domain 1 (domain) [3-125]
- IPR027408: PNPase/RNase PH domain superfamily (homologous_superfamily) [1-223]
- IPR020568: Ribosomal protein uS5 domain 2-type superfamily (homologous_superfamily) [4-125]
- IPR050080: Ribonuclease PH (family) [3-211]
- IPR015847: Exoribonuclease, phosphorolytic domain 2 (domain) [131-200]
Interaction partners: - Superkiller protein 7
- ATP-dependent RNA helicase DOB1
- Exosome complex component RRP40
- Exosome complex component CSL4
- Exosome complex exonuclease RRP6
- Exosome complex component RRP4
- Exosome complex exonuclease DIS3
- Exosome complex component RRP42
- Exosome complex protein LRP1
- Pre-rRNA-processing protein PNO1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0008152 (metabolic process), GO:0048519 (negative regulation of biological process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0009892 (negative regulation of metabolic process), GO:0065008 (regulation of biological quality), GO:0050794 (regulation of cellular process), GO:0048523 (negative regulation of cellular process), GO:0019222 (regulation of metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044237 (cellular metabolic process), GO:0009893 (positive regulation of metabolic process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0043487 (regulation of RNA stability), GO:0044085 (cellular component biogenesis), GO:0044248 (cellular catabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0009894 (regulation of catabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0009896 (positive regulation of catabolic process), GO:0044270 (cellular nitrogen compound catabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0043488 (regulation of mRNA stability), GO:0019439 (aromatic compound catabolic process), GO:0046700 (heterocycle catabolic process), GO:0010468 (regulation of gene expression), GO:0009057 (macromolecule catabolic process), GO:0090304 (nucleic acid metabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0010467 (gene expression), GO:0010629 (negative regulation of gene expression), GO:0034249 (negative regulation of amide metabolic process), GO:0034655 (nucleobase-containing compound catabolic process), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0051248 (negative regulation of protein metabolic process), GO:0050779 (RNA destabilization), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0034248 (regulation of amide metabolic process), GO:0044265 (cellular macromolecule catabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0031331 (positive regulation of cellular catabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031329 (regulation of cellular catabolic process), GO:0016070 (RNA metabolic process), GO:0061013 (regulation of mRNA catabolic process), GO:0090305 (nucleic acid phosphodiester bond hydrolysis), GO:0042254 (ribosome biogenesis), GO:2000112 (regulation of cellular macromolecule biosynthetic process), GO:1903311 (regulation of mRNA metabolic process), GO:0017148 (negative regulation of translation), GO:0061014 (positive regulation of mRNA catabolic process), GO:0006402 (mRNA catabolic process), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0006401 (RNA catabolic process), GO:0006396 (RNA processing), GO:0006417 (regulation of translation), GO:0061157 (mRNA destabilization), GO:2000113 (negative regulation of cellular macromolecule biosynthetic process), GO:1903313 (positive regulation of mRNA metabolic process), GO:0010608 (post-transcriptional regulation of gene expression), GO:0090501 (RNA phosphodiester bond hydrolysis), GO:0071025 (RNA surveillance), GO:0006364 (rRNA processing), GO:0016071 (mRNA metabolic process), GO:0034660 (ncRNA metabolic process), GO:0034470 (ncRNA processing), GO:0031123 (RNA 3'-end processing), GO:0000956 (nuclear-transcribed mRNA catabolic process), GO:0043633 (polyadenylation-dependent RNA catabolic process), GO:0034661 (ncRNA catabolic process), GO:0000460 (maturation of 5.8S rRNA), GO:0000469 (cleavage involved in rRNA processing), GO:0016180 (snRNA processing), GO:0031125 (rRNA 3'-end processing), GO:0034472 (snRNA 3'-end processing), GO:0016072 (rRNA metabolic process), GO:0090503 (RNA phosphodiester bond hydrolysis, exonucleolytic), GO:0016078 (tRNA catabolic process), GO:0006399 (tRNA metabolic process), GO:0016073 (snRNA metabolic process), GO:0043634 (polyadenylation-dependent ncRNA catabolic process), GO:0043144 (sno(s)RNA processing), GO:0000291 (nuclear-transcribed mRNA catabolic process, exonucleolytic), GO:0016074 (sno(s)RNA metabolic process), GO:0000184 (nuclear-transcribed mRNA catabolic process, nonsense-mediated decay), GO:0071028 (nuclear mRNA surveillance), GO:0071027 (nuclear RNA surveillance), GO:0070481 (nuclear-transcribed mRNA catabolic process, non-stop decay), GO:0016075 (rRNA catabolic process), GO:0071029 (nuclear ncRNA surveillance), GO:0043628 (regulatory ncRNA 3'-end processing), GO:0031126 (sno(s)RNA 3'-end processing), GO:0071046 (nuclear polyadenylation-dependent ncRNA catabolic process), GO:0000459 (exonucleolytic trimming involved in rRNA processing), GO:0000466 (maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA))
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:1902494 (catalytic complex), GO:0140513 (nuclear protein-containing complex), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0043233 (organelle lumen), GO:0000176 (nuclear exosome (RNase complex)), GO:0000178 (exosome (RNase complex)), GO:1905354 (exoribonuclease complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0000177 (cytoplasmic exosome (RNase complex)), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus)