Protein: Glutathione hydrolase proenzyme 1 (EC 3.4.19.13) (Gamma-glutamyltransferase 1) (Gamma-glutamyltranspeptidase 1) (EC 2.3.2.2) [Cleaved into: Glutathione hydrolase 1 heavy chain; Glutathione hydrolase 1 light chain] (Q9US04)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Catalyzes the transfer of the gamma-glutamyl moiety of glutathione (GSH) and other gamma-glutamyl compounds to amino acids and peptides. Major GSH-degrading enzyme, catalyzing the hydrolytic release of L-glutamate from GSH.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
Sequence: MGINTSSAQSSGAASIARSSVNVKSGNRHLSSNKKSATSALEERASRPSILVTFLVLAGTILSLYIWPILSPDLFFANQRCSFKYKNKGSQRVVVEGKNGVVATEEETCSQIGVGILKAGGNAVDAAIASGICIGAVNSFSSGIGGGGFMLIRHPNGTAHSLNFRETAPAGASKNMFHGNSTLSQVGGLSVAVPGEIAGYERAWKMYGSLPWHKLFEPTIRLMRDGMPMPKELASRIRRPEFSYFKTHPDWSKIFAPEGVFLHVGEKFYRPALASTLEEIAKFGPEVFYTGKIAERLVKFVQQQGGILTMEDMANFSVVVEEPIYGNFYDREVITCGSPCSGEALILGLNVLSKVDLSEGTSILGCEMTDIGVHHLIETMKWMSAGRTVLADPTFYNNTDHVEQLLSLEYADEIRNNISNERTFDFTHYKAEYDFPNDHGTTHLSVIDKDNMAVGLTASINLMFGSQLLEPETGIILNDHMDDFASPGIVNAFGLSPSPYNFIAPGKRPQSSAVPTILVYNGEVEMVLGGSGGSRIVTAVLDTIIKKYKWGKSLLESVESPRFHHQLMPNIVYIDETVEIEVLRALEKFGHIVDLIPVQYPFSEIQAVFRTNGTLYGLSDSRKQAVAAAY
InterPro domains: - IPR043137: Gamma-glutamyltranspeptidase, small subunit, C-terminal domain (homologous_superfamily) [435-630]
- IPR029055: Nucleophile aminohydrolases, N-terminal (homologous_superfamily) [92-630]
- IPR043138: Gamma-glutamyltranspeptidase, large subunit (homologous_superfamily) [323-434]
- IPR000101: Gamma-glutamyltranspeptidase (family) [75-630]
Interaction partners: - Cys-Gly metallodipeptidase dug1
- Glutathione synthetase large chain
- Putative aminopeptidase C13A11.05
- Glutamate--cysteine ligase
- Putative glutamate--cysteine ligase regulatory subunit
- Glutathione reductase
- Glutathione S-transferase 3
- Glutathione S-transferase 2
- Glutathione S-transferase 1
- Glutathione hydrolase proenzyme 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0008233 (peptidase activity), GO:0008242 (omega peptidase activity), GO:0070003 (threonine-type peptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0051716 (cellular response to stimulus), GO:0009056 (catabolic process), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0071704 (organic substance metabolic process), GO:1901575 (organic substance catabolic process), GO:0006749 (glutathione metabolic process), GO:0009410 (response to xenobiotic stimulus), GO:0070887 (cellular response to chemical stimulus), GO:0006805 (xenobiotic metabolic process), GO:0071466 (cellular response to xenobiotic stimulus)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)