Protein: Alpha-(1,3)-fucosyltransferase 4 (4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase) (EC 2.4.1.152) (Fucosyltransferase 4) (Fucosyltransferase IV) (Fuc-TIV) (FucT-IV) (Galactoside 3-L-fucosyltransferase) (Q11127)
Organism: Mus musculus (Mouse)
Function: Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, Gal-beta(1->4)GlcNAc) glycan attached to N- or O-linked glycoproteins. Robustly fucosylates nonsialylated distal LacNAc unit of the polylactosamine chain to form Lewis X antigen (CD15), a glycan determinant known to mediate important cellular functions in development and immunity. Fucosylates with lower efficiency sialylated LacNAc acceptors to form sialyl Lewis X and 6-sulfo sialyl Lewis X determinants that serve as recognition epitopes for C-type lectins. Together with FUT7 contributes to SELE, SELL and SELP selectin ligand biosynthesis and selectin-dependent lymphocyte homing, leukocyte migration and blood leukocyte homeostasis. In a cell type specific manner, may also fucosylate the internal LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE (By similarity).
Subcellular location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
Sequence: MAPARQELQHESRCRPSRTVDAWRAAVATRGRHMETPGYRRRTRCGGWGLPRSVSSLAAVGLLCTALTTFICWGQLPPLPWASPAPQRLVGVLLWWEPFRGRGGYPKSPPDCSLRFNISGCRLLTDRAAYGEAQAVLFHHRDLVKELHDWPPPWGARERTDKALVLRVFDDQEGAVTLTGKALETVGSRPPGQRWVWMNFESPSHTPGLRGLAKDLFNWTLSYRTDSDVFVPYGFLYSRSDPTEQPSGLGPQLARKRGLVAWVVSNWNEHQARVRYYHQLSRHVSVDVFGRTGPGRPVPAIGLLHTVARYKFYLAFENSRHVDYITEKLWRNAFLAGAVPVVLGPDRANYERFVPRGAFIHVDDFPNAASLAAYLLFLDRNVAVYRRYFRWRRSFAVHITSFWDEQWCRTCQAVQTSGDQPKSIHNLADWFQR
InterPro domains: - IPR055270: Fucosyltransferase, C-terminal (domain) [254-430]
- IPR001503: Glycosyl transferase family 10 (family) [87-431]
- IPR031481: Fucosyltransferase, N-terminal (domain) [91-234]
- IPR038577: GT10-like, C-terminal domain superfamily (homologous_superfamily) [197-404]
Interaction partners: - Galactoside alpha-
- Galactoside alpha-
- E-selectin
- Type 2 lactosamine alpha-2,3-sialyltransferase
- N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3
- Galactoside 2-alpha-L-fucosyltransferase Sec1
- Beta-1,4-galactosyltransferase 1
- Beta-1,4-galactosyltransferase 4
- N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2
- Beta-1,4-galactosyltransferase 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016757 (glycosyltransferase activity), GO:0016758 (hexosyltransferase activity), GO:0008417 (fucosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0009058 (biosynthetic process), GO:0048870 (cell motility), GO:0006807 (nitrogen compound metabolic process), GO:0070085 (glycosylation), GO:0071704 (organic substance metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0008037 (cell recognition), GO:0044238 (primary metabolic process), GO:0040012 (regulation of locomotion), GO:0048522 (positive regulation of cellular process), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:2000145 (regulation of cell motility), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0019538 (protein metabolic process), GO:0030155 (regulation of cell adhesion), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0009988 (cell-cell recognition), GO:0045785 (positive regulation of cell adhesion), GO:0005975 (carbohydrate metabolic process), GO:0043170 (macromolecule metabolic process), GO:0043413 (macromolecule glycosylation), GO:1901135 (carbohydrate derivative metabolic process), GO:0042127 (regulation of cell population proliferation), GO:0006629 (lipid metabolic process), GO:0036065 (fucosylation), GO:0043603 (amide metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0022409 (positive regulation of cell-cell adhesion), GO:0022407 (regulation of cell-cell adhesion), GO:0016051 (carbohydrate biosynthetic process), GO:0030334 (regulation of cell migration), GO:0006665 (sphingolipid metabolic process), GO:0036211 (protein modification process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006486 (protein glycosylation), GO:0043412 (macromolecule modification), GO:0009311 (oligosaccharide metabolic process), GO:0009100 (glycoprotein metabolic process), GO:0006643 (membrane lipid metabolic process), GO:0006672 (ceramide metabolic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0009312 (oligosaccharide biosynthetic process), GO:0006487 (protein N-linked glycosylation), GO:0006493 (protein O-linked glycosylation), GO:0009101 (glycoprotein biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)