Protein: FADH(2)-dependent monooxygenase TftD (EC 1.14.14.-) (Chlorophenol-4-monooxygenase component 2) (Two component enzyme D) (O87009)
Organism: Burkholderia cepacia (Pseudomonas cepacia)
Function: Oxygenase component of a two-component system that degrades 2,4,5-trichlorophenol. Uses FADH(2) supplied by TftC to oxidize 2,4,5-trichlorophenol (2,4,5-TCP) to 2,5-dichloro-p-benzoquinone, which is chemically reduced to 2,5-dichloro-p-hydroquinone (2,5-DiCHQ). Then, TftD oxidizes the latter to 5-chloro-2-hydroxy-p-benzoquinone.
Sequence: MRTGKQYLESLNDGRVVWVGNEKIDNVATHPLTRDYAERVAQFYDLHHRPDLQDVLTFVDADGVRRSRQWQDPKDAAGLRVKRKYHETILREIAAGSYGRLPDAHNYTFTTYADDPEVWEKQSIGAEGRNLTQNIHNFLKLLREKDLNCPLNFVDPQTDRSSDAAQARSPNLRIVEKTDDGIIVNGVKAVGTGIAFGDYMHIGCLYRPGIPGEQVIFAAIPTNTPGVTVFCRESTVKNDPAEHPLASQGDELDSTTVFDNVFIPWEQVFHIGNPEHAKLYPQRIFDWVHYHILIRQVLRAELIVGLAILITEHIGTSKLPTVSARVAKLVAFHLAMQAHLIASEETGFHTKGGRYKPNPLIYDFGRAHFLQNQMSVMYELLDLAGRSSLMIPSEGQWDDSQSGQWFVKLNNGPKGNPRERVQIGRVIRDLYLTDWGGRQFMFENFNGTPLFAVFAATMTRDDMSAAGTYGKFASQVCGIEFGGAEPTAYAATADYAKALDKGLAPEPAAAESATS
InterPro domains: - IPR036250: Acyl-CoA dehydrogenase-like, C-terminal (homologous_superfamily) [261-479]
- IPR024719: HpaB/PvcC/4-BUDH C-terminal (domain) [285-457]
- IPR004925: HpaB/PvcC/4-BUDH (family) [1-444]
- IPR009100: Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain superfamily (homologous_superfamily) [1-269]
- IPR024674: HpaB/PvcC/4-BUDH N-terminal (domain) [3-269]
- IPR046373: Acyl-CoA oxidase/dehydrogenase, middle domain superfamily (homologous_superfamily) [148-272]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0016491 (oxidoreductase activity), GO:0004497 (monooxygenase activity), GO:1901265 (nucleoside phosphate binding), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0046983 (protein dimerization activity), GO:0043168 (anion binding), GO:0042802 (identical protein binding), GO:0000166 (nucleotide binding), GO:0050660 (flavin adenine dinucleotide binding), GO:0042803 (protein homodimerization activity), GO:0016709 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen), GO:0071949 (FAD binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0051716 (cellular response to stimulus), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0006807 (nitrogen compound metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006805 (xenobiotic metabolic process), GO:1901575 (organic substance catabolic process), GO:0009410 (response to xenobiotic stimulus), GO:0070887 (cellular response to chemical stimulus), GO:0044248 (cellular catabolic process), GO:0071466 (cellular response to xenobiotic stimulus), GO:1901616 (organic hydroxy compound catabolic process), GO:0018958 (phenol-containing compound metabolic process), GO:0042178 (xenobiotic catabolic process), GO:0019439 (aromatic compound catabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0019336 (phenol-containing compound catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)