Protein: GDP-fucose protein O-fucosyltransferase 3 (EC 2.4.1.221) (Alpha-(1,3)-fucosyltransferase 10) (EC 2.4.1.-) (Fucosyltransferase X) (Fuc-TX) (FucT-X) (Galactoside 3-L-fucosyltransferase 10) (Fucosyltransferase 10) (Q6P4F1)
Organism: Homo sapiens (Human)
Function: Predominantly fucosylates the innermost N-acetyl glucosamine (GlcNAc) residue in biantennary N-glycan acceptors. Postulated to generate core alpha(1->3)-fucose epitope within the chitobiose unit of biantennary N-glycans, providing for a recognition signal to reorient aberrantly folded glycoproteins for degradation. Involved in biosynthesis of Lewis X-carrying biantennary N-glycans that regulate neuron stem cell self-renewal during brain development (By similarity).
Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:39775168}; Single-pass type II membrane protein {ECO:0000255}.; SUBCELLULAR LOCATION: [Isoform 1]: Endoplasmic reticulum membrane {ECO:0000305|PubMed:19088067}; Single-pass type II membrane protein {ECO:0000255}. Golgi apparatus membrane {ECO:0000305|PubMed:12370785, ECO:0000305|PubMed:19088067}; Single-pass type II membrane protein {ECO:0000255}.; SUBCELLULAR LOCATION: [Isoform 4]: Golgi apparatus {ECO:0000269|PubMed:19088067}. Lysosome {ECO:0000269|PubMed:19088067}.; SUBCELLULAR LOCATION: [Isoform 5]: Endoplasmic reticulum membrane {ECO:0000269|PubMed:19088067}; Single-pass type II membrane protein {ECO:0000255}. Golgi apparatus membrane {ECO:0000305|PubMed:19088067}; Single-pass type II membrane protein {ECO:0000255}.
Sequence: MVRIQRRKLLASCLCVTATVFLLVTLQVMVELGKFERKEFKSSSLQDGHTKMEEAPTHLNSFLKKEGLTFNRKRKWELDSYPIMLWWSPLTGETGRLGQCGADACFFTINRTYLHHHMTKAFLFYGTDFNIDSLPLPRKAHHDWAVFHEESPKNNYKLFHKPVITLFNYTATFSRHSHLPLTTQYLESIEVLKSLRYLVPLQSKNKLRKRLAPLVYVQSDCDPPSDRDSYVRELMTYIEVDSYGECLRNKDLPQQLKNPASMDADGFYRIIAQYKFILAFENAVCDDYITEKFWRPLKLGVVPVYYGSPSITDWLPSNKSAILVSEFSHPRELASYIRRLDSDDRLYEAYVEWKLKGEISNQRLLTALRERKWGVQDVNQDNYIDAFECMVCTKVWANIRLQEKGLPPKRWEAEDTHLSCPEPTVFAFSPLRTPPLSSLREMWISSFEQSKKEAQALRWLVDRNQNFSSQEFWGLVFKD
InterPro domains: - IPR017176: Alpha-(1, 3)-fucosyltransferase, metazoan (family) [1-479]
- IPR031481: Fucosyltransferase, N-terminal (domain) [83-180]
- IPR055270: Fucosyltransferase, C-terminal (domain) [213-362]
- IPR038577: GT10-like, C-terminal domain superfamily (homologous_superfamily) [146-365]
- IPR001503: Glycosyl transferase family 10 (family) [72-408]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0016757 (glycosyltransferase activity), GO:0016758 (hexosyltransferase activity), GO:0008417 (fucosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009058 (biosynthetic process), GO:0048870 (cell motility), GO:0070085 (glycosylation), GO:0098727 (maintenance of cell number), GO:0051301 (cell division), GO:0071704 (organic substance metabolic process), GO:0019827 (stem cell population maintenance), GO:0044238 (primary metabolic process), GO:0097150 (neuronal stem cell population maintenance), GO:0021987 (cerebral cortex development), GO:1901576 (organic substance biosynthetic process), GO:0036065 (fucosylation), GO:0021537 (telencephalon development), GO:0060322 (head development), GO:0030900 (forebrain development), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0021543 (pallium development), GO:0005975 (carbohydrate metabolic process), GO:0016477 (cell migration), GO:0017145 (stem cell division), GO:0007420 (brain development), GO:0007399 (nervous system development), GO:0097402 (neuroblast migration), GO:0009311 (oligosaccharide metabolic process), GO:0048103 (somatic stem cell division), GO:0016051 (carbohydrate biosynthetic process), GO:0007417 (central nervous system development), GO:0021885 (forebrain cell migration), GO:0022029 (telencephalon cell migration), GO:0021795 (cerebral cortex cell migration), GO:0021799 (cerebral cortex radially oriented cell migration), GO:0009312 (oligosaccharide biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0016020 (membrane), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0005794 (Golgi apparatus), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0000139 (Golgi membrane), GO:0043231 (intracellular membrane-bounded organelle)