Protein: Leucine aminopeptidase (EC 3.4.11.-) (Epoxide hydrolase) (EC 3.3.2.10) (Leukotriene A-4 hydrolase homolog) (LTA-4 hydrolase) (Q9FY49)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Aminopeptidase that preferentially cleaves di- and tripeptides. Also has low epoxide hydrolase activity (in vitro). Can hydrolyze the epoxide leukotriene LTA(4) but it forms preferentially 5,6-dihydroxy-7,9,11,14-eicosatetraenoic acid rather than the cytokine leukotriene B(4) as the product compared to the homologous mammalian enzyme (in vitro).
Subcellular location: Cytoplasm {ECO:0000250}.
Sequence: MAPIDPHSFTDSSHPLTTHVALSLYLDFNTSIIHGSALLTLSSAFSGELSLDTRCISIAMVLDPLTLEPIPYSVSTTPDRIRGTEVVVVLSGQSSLLIVYSTSPSASALQWLSPLQTFSKLHPYVYTQCQAIHARSIFPCQDTPAARIRYDVVMNIPNSLSAVMSARHVRRRLAVPEEAKHLEAGSLGSSLWCGEDRVVEEFAMEQPIPPYLFAFAVGELGFREVGPRTRVYTESAAIEVLDAAALEFAGTEDMIKQGEKLFGDYEWERFDLLVLPPSFPYGGMENPRMVFLTPTVIKGDATGAQVVAHELAHSWTGNLITNINNEHFWLNEGFTTYAERRIVEVVQGADIATLNIGIGWRGLTDEMERFKDNLECTKLWNKQEGVDPDDVYSQVPYEKGFQFVLRIERQIGRTAFDEFLKKYIATFKFKSIDTNTFLEFLKANIPGIEKEINLQLWTEGVGIPEDAYEPVSTIYTKIISLAKEFKEGKMPSEDDVAEWNGQEWELYLENLPKSCEPSQVMALDKRYRLAESKDYEVKVSFLQLAVTSKCREYHGEVKKTLKEVGRMKYLRPLFTALAQSGGTEEKQLAKQVFAEARETYHPIAQGVVESILSKYI
InterPro domains: - IPR016024: Armadillo-type fold (homologous_superfamily) [485-612]
- IPR001930: Peptidase M1, alanine aminopeptidase/leukotriene A4 hydrolase (family) [135-337]
- IPR034015: Aminopeptidase, leukotriene A4 hydrolase-like (family) [2-614]
- IPR045357: Aminopeptidase N-like , N-terminal domain (domain) [19-165]
- IPR049980: Leukotriene A4 hydrolase-like, catalytic domain (domain) [5-457]
- IPR015211: Peptidase M1, leukotriene A4 hydrolase/aminopeptidase C-terminal (domain) [473-612]
- IPR014782: Peptidase M1, membrane alanine aminopeptidase (domain) [255-443]
- IPR042097: Aminopeptidase N-like , N-terminal domain superfamliy (homologous_superfamily) [4-219]
- IPR027268: Peptidase M4/M1, CTD superfamily (homologous_superfamily) [319-468]
- IPR038502: Peptidase M1, LTA-4 hydrolase/aminopeptidase, C-terminal domain superfamily (homologous_superfamily) [472-613]
Interaction partners: - Leucine aminopeptidase 2, chloroplastic
- Transcription initiation factor TFIID subunit 2
- Threonine dehydratase biosynthetic, chloroplastic
- beta-galactosidase
- Leucine aminopeptidase 3, chloroplastic
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0008233 (peptidase activity), GO:0008238 (exopeptidase activity), GO:0004177 (aminopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009605 (response to external stimulus), GO:0009058 (biosynthetic process), GO:0006950 (response to stress), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0051707 (response to other organism), GO:0009607 (response to biotic stimulus), GO:0044238 (primary metabolic process), GO:0006952 (defense response), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:1901576 (organic substance biosynthetic process), GO:0043207 (response to external biotic stimulus), GO:0006629 (lipid metabolic process), GO:0009620 (response to fungus), GO:0098542 (defense response to other organism), GO:0006720 (isoprenoid metabolic process), GO:0008299 (isoprenoid biosynthetic process), GO:0050832 (defense response to fungus), GO:0008610 (lipid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)