Protein: Acyl-CoA wax alcohol acyltransferase 2 (EC 2.3.1.75) (11-cis-specific retinyl-ester synthase) (11-cis-RE-synthase) (Acyl-CoA retinol O-fatty-acyltransferase) (ARAT) (Retinol O-fatty-acyltransferase) (EC 2.3.1.76) (Diacylglycerol O-acyltransferase 2-like protein 4) (EC 2.3.1.20) (Long-chain-alcohol O-fatty-acyltransferase 2) (Wax synthase) (mWS) (Q6E1M8)
Organism: Mus musculus (Mouse)
Function: Acyltransferase that catalyzes the formation of ester bonds between fatty alcohols and fatty acyl-CoAs to form wax monoesters. Shows a preference for medium chain acyl-CoAs from C12 to C16 in length and fatty alcohols shorter than C20, as the acyl donor and acceptor, respectively. Also possesses acyl-CoA retinol acyltransferase (ARAT) activity that catalyzes 11-cis-specific retinyl ester synthesis. Shows higher catalytic efficiency toward 11-cis-retinol versus 9-cis-retinol, 13- cis-retinol and all-trans-retinol substrates (By similarity).
Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:15220349}; Multi-pass membrane protein {ECO:0000255}.
Sequence: MFWPTKKDLKTAMEVFALFQWALSALVIVTTVIIVNLYLVVFTSYWPVTVLMLTWLAFDWKTPERGGRRFTCVRKWRLWKHYSDYFPLKMVKTKDISPDRNYILVCHPHGLMAHSCFGHFATDTTGFSKTFPGITPYMLTLGAFFWVPFLRDYVMSTGSCSVSRSSMDFLLTQKGTGNMLVVVVGGLAECRYSTPGSTTLFLKKRQGFVRTALKHGVSLIPAYAFGETDLYDQHIFTPGGFVNRFQKWFQKMVHIYPCAFYGRGLTKNSWGLLPYSQPVTTVVGEPLPLPKIENPSEEIVAKYHTLYIDALRKLFDQHKTKFGISETQELVIV
InterPro domains: - IPR007130: Diacylglycerol acyltransferase (family) [39-332]
Interaction partners: - Diacylglycerol O-acyltransferase 1
- Retinol dehydrogenase 11
- Cytochrome P450 26A1
- Alcohol dehydrogenase 1
- Alcohol dehydrogenase class-3
- All-trans-retinol dehydrogenase [NAD
- All-trans-retinol dehydrogenase [NAD
- 17-beta-hydroxysteroid dehydrogenase type 6
- Epidermal retinol dehydrogenase 2
- Retinol dehydrogenase 5
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016746 (acyltransferase activity), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups), GO:0008374 (O-acyltransferase activity), GO:0016411 (acylglycerol O-acyltransferase activity), GO:0004144 (diacylglycerol O-acyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0007586 (digestion), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0051234 (establishment of localization), GO:0071704 (organic substance metabolic process), GO:0003008 (system process), GO:0044238 (primary metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0022600 (digestive system process), GO:0006810 (transport), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0046460 (neutral lipid biosynthetic process), GO:0046486 (glycerolipid metabolic process), GO:0050892 (intestinal absorption), GO:0006638 (neutral lipid metabolic process), GO:0008610 (lipid biosynthetic process), GO:0045017 (glycerolipid biosynthetic process), GO:0046463 (acylglycerol biosynthetic process), GO:0006639 (acylglycerol metabolic process), GO:0046339 (diacylglycerol metabolic process), GO:0006641 (triglyceride metabolic process), GO:0019432 (triglyceride biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0016020 (membrane), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)