Protein: KH domain-containing, RNA-binding, signal transduction-associated protein 1 (Src-associated in mitosis 68 kDa protein) (Sam68) (Q8UUW7)
Organism: Gallus gallus (Chicken)
Function: Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion.
Subcellular location: Nucleus {ECO:0000250|UniProtKB:Q07666}. Cytoplasm {ECO:0000250|UniProtKB:Q07666}. Membrane {ECO:0000250|UniProtKB:Q07666}. Note=Predominantly located in the nucleus but also located partially in the cytoplasm. {ECO:0000250|UniProtKB:Q07666}.
Sequence: MQRRDDSSARMGRGPGGPGSARQGGPNPRRSPRGGGGRGAGAQHPQPLLTGGAAAGSSGAQGPAAANPAPLLPGGAVKMEPENKYLPELMAEKDSLDPSSTHAMQLLSAEIEKIQKGETTKKDEEENYLDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLNGVPEPTRGRGGPVRGRGAAPPPPPPVPRGRGVGPPPPPPPPRGALVRGAPVRGAIARGAAVARGVPPPPAVRGAPAPRARAAGIQRIPLPPPPAPETYEEYGYDDAYADQSYEGYEGYYSQGQGDTEYYDYGHGEAQETYEAYGQDDWNGTRPSLKAPPARPVKGAYREHPYGRY
InterPro domains: - IPR032335: Sam68, tyrosine-rich domain (domain) [356-405]
- IPR036612: K Homology domain, type 1 superfamily (homologous_superfamily) [76-265]
- IPR045071: KH domain-containing BBP-like (family) [43-401]
- IPR004087: K Homology domain (domain) [137-235]
- IPR055256: KHDC4/BBP-like, KH-domain type I (domain) [149-215]
- IPR032571: KHDRBS, Qua1 domain (domain) [84-136]
Interaction partners: - Heterogeneous nuclear ribonucleoprotein K
- Heterogeneous nuclear ribonucleoprotein K
- Proto-oncogene tyrosine-protein kinase Src
- Uncharacterized protein.
- Serine/arginine-rich splicing factor 1
- Heterogeneous nuclear ribonucleoprotein M
- Growth factor receptor-bound protein 2
- U2 small nuclear RNA auxiliary factor 2.
- Serine/arginine-rich splicing factor 2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0042802 (identical protein binding), GO:0019904 (protein domain specific binding), GO:0003723 (RNA binding), GO:0017124 (SH3 domain binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0019953 (sexual reproduction), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0051716 (cellular response to stimulus), GO:0032504 (multicellular organism reproduction), GO:0003006 (developmental process involved in reproduction), GO:0048609 (multicellular organismal reproductive process), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0007276 (gamete generation), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0007166 (cell surface receptor signaling pathway), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0007283 (spermatogenesis), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:0048232 (male gamete generation), GO:0010468 (regulation of gene expression), GO:0043484 (regulation of RNA splicing), GO:1903311 (regulation of mRNA metabolic process), GO:0050684 (regulation of mRNA processing), GO:0048024 (regulation of mRNA splicing, via spliceosome), GO:0000381 (regulation of alternative mRNA splicing, via spliceosome)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)