Protein: Pre-mRNA-processing-splicing factor 8 (A0A8M2B4I2)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as a scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site
Subcellular location: Nucleus
Sequence: MAAPFPYRGLPGPMPPGVPPPPPAVAPVPDYMTEEKLQEKARKWQQLQAKRYSEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGTMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQMELDPDEDSSVTDWLYEHQPLKDTTKYVNGTTYRRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEYVEPFLKETPLYTDNTANGIALLWAPRPFNLRSGRTRRAIDVPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLSKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAPGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSESWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAIYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETAEGECNVMLESRYEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDTATESAHPIRLYCRYIDRIHIFFRFSADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTIQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKFWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGAKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGTSPPDNPQVKEIRCIVMVPQWGTHQTVHLPNQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKVMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPGQVSWNYNFMGVRHDPNMKYDLQLANPKEFYHEVHRPSHFLNFASLQEGEIYNADREDMYA
InterPro domains: - IPR012592: PROCN domain (domain) [403-808]
- IPR012591: PRO8NT domain (domain) [65-216]
- IPR019580: Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding (domain) [1449-1607]
- IPR021983: PRP8 domain IV core (domain) [1767-2023]
- IPR019582: RNA recognition motif, spliceosomal PrP8 (domain) [993-1083]
- IPR000555: JAB1/MPN/MOV34 metalloenzyme domain (domain) [2106-2240]
- IPR019581: Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding (domain) [1244-1379]
- IPR043173: Prp8 RNase domain IV, fingers region (homologous_superfamily) [1929-2027]
- IPR012984: PROCT domain (domain) [2219-2339]
- IPR037518: MPN domain (domain) [2110-2241]
- IPR043172: Prp8 RNase domain IV, palm region (homologous_superfamily) [1768-1928]
- IPR042516: Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain superfamily (homologous_superfamily) [1191-1310]
- IPR027652: Pre-mRNA-processing-splicing factor 8 (family) [29-2331]
- IPR012337: Ribonuclease H-like superfamily (homologous_superfamily) [1004-2022]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0048880 (sensory system development), GO:0007423 (sensory organ development), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0001654 (eye development), GO:0006396 (RNA processing), GO:0150063 (visual system development), GO:0008380 (RNA splicing), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0043010 (camera-type eye development), GO:0000398 (mRNA splicing, via spliceosome), GO:0000375 (RNA splicing, via transesterification reactions), GO:0000377 (RNA splicing, via transesterification reactions with bulged adenosine as nucleophile)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)