Protein: Demethyldecarbamoylnovobiocin O-methyltransferase (EC 2.1.1.285) (Novobiocin biosynthesis protein P) (Q9L9F2)
Organism: Streptomyces niveus (Streptomyces spheroides)
Function: S-adenosyl-L-methionine-dependent O-methyltransferase that methylates at 4-OH of the noviose moiety, the penultimate step in the novobiocin biosynthesis pathway. Novobiocin is an aminocoumarin family antibiotic that targets bacterial DNA gyrases.
Sequence: MAPIVETAKETNSDSSLYLDLMIKVLAGTVYEDPAHRENFSHRDSTYREEVRNEGRDWPANAHTMIGIKRLENIRQCVEDVIGNNVPGDLVETGVWRGGACILMRGILRAHDVRDRTVWVADSFQGIPDVGEDGYAGDRKMALHRRNSVLAVSEEEVRRNFRNYDLLDEQVRFLPGWFKDTLPTAPIDTLAVLRMDGDLYESTWDTLTNLYPKVSVGGYVIVDDYMMCPPCKDAVDEYRAKFDIADELITIDRDGVYWQRTR
InterPro domains: - IPR008884: Macrocin-O-methyltransferase (family) [18-261]
- IPR029063: S-adenosyl-L-methionine-dependent methyltransferase superfamily (homologous_superfamily) [14-262]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0008168 (methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0016999 (antibiotic metabolic process), GO:0044281 (small molecule metabolic process), GO:0032259 (methylation), GO:0006807 (nitrogen compound metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:0017000 (antibiotic biosynthetic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0009698 (phenylpropanoid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:0018904 (ether metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009804 (coumarin metabolic process), GO:1901503 (ether biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:1901657 (glycosyl compound metabolic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0009699 (phenylpropanoid biosynthetic process), GO:0018958 (phenol-containing compound metabolic process), GO:1901659 (glycosyl compound biosynthetic process), GO:0009805 (coumarin biosynthetic process), GO:0016137 (glycoside metabolic process), GO:0046189 (phenol-containing compound biosynthetic process), GO:0016138 (glycoside biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)