Protein: F-box protein MET30 (E3 ubiquitin ligase complex SCF(Met30) subunit MET30) (Methionine-requiring protein 30) (P39014)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Substrate-recognition component of the SCF(Met30) complex, an E3 ubiquitin ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Negatively regulates sulfur amino acids biosynthesis genes expression. Controls cell cycle function (being required for the G1/S transition and M-phase but not the S-phase), sulfur metabolism, and methionine biosynthesis as part of the SCF(Met30) complex. Required for the efficient binding of CDC45 and MCM proteins to origins of replication. Required for efficient expression of G1 cyclins. The SCF(Met30) complex catalyzes ubiquitination and degradation of the Cdk-inhibitory kinase SWE1. Involved in the S-adenosylmethionine (AdoMet)-mediated inhibition of the transcription function of MET4. The SCF(Met30) complex mediates ubiquitination and subsequent degradation of MET4 and the cellular response to cadmium. The SCF(Met30) complex acts as an inhibitor of autophagy by promoting ubiquitination and degradation of ATG9 in normal conditions.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:14660673}. Nucleus {ECO:0000269|PubMed:10637232, ECO:0000269|PubMed:14660673}.
Sequence: MRRERQRMMSFEDKDKDDLDNSNSNNSSEMTDTAMMPPLKRLLITGSSDDLAQGSSGKKKMTMATRSPSSSPDLATNDSGTRVQPLPEYNFTKFCYRHNPDIQFSPTHTACYKQDLKRTQEINANIAKLPLQEQSDIHHIISKYSNSNDKIRKLILDGILSTSCFPQLSYISSLVTHMIKIDFISILPQELSLKILSYLDCQSLCNATRVCRKWQKLADDDRVWYHMCEQHIDRKCPNCGWGLPLLHMKRARIQQNSTGSSSNADIQTQTTRPWKVIYRERFKVESNWRKGHCRIQEFKGHMDGVLTLQFNYRLLFTGSYDSTIGIWDLFTGKLIRRLSGHSDGVKTLYFDDRKLITGSLDKTIRVWNYITGECISTYRGHSDSVLSVDSYQKVIVSGSADKTVKVWHVESRTCYTLRGHTEWVNCVKLHPKSFSCFSCSDDTTIRMWDIRTNSCLKVFRGHVGQVQKIIPLTIKDVENLATDNTSDGSSPQDDPTMTDGADESDTPSNEQETVLDENIPYPTHLLSCGLDNTIKLWDVKTGKCIRTQFGHVEGVWDIAADNFRIISGSHDGSIKVWDLQSGKCMHTFNGRRLQRETQHTQTQSLGDKVAPIACVCIGDSECFSGDEFGCVKMYKFDLND
InterPro domains: - IPR019775: WD40 repeat, conserved site (conserved_site) [315-579]
- IPR001680: WD40 repeat (repeat) [291-587]
- IPR020472: PAC1/LIS1-like, WD-40 repeat (repeat) [355-579]
- IPR015943: WD40/YVTN repeat-like-containing domain superfamily (homologous_superfamily) [288-639]
- IPR036047: F-box-like domain superfamily (homologous_superfamily) [180-291]
- IPR001810: F-box domain (domain) [181-229]
- IPR051075: SCF complex subunit WD repeat-containing protein (family) [88-592]
- IPR036322: WD40-repeat-containing domain superfamily (homologous_superfamily) [298-636]
Interaction partners: - Suppressor of kinetochore protein 1
- Cell division control protein 53
- Transcriptional activator of sulfur metabolism MET4
- RING-box protein HRT1
- SCF E3 ubiquitin ligase complex F-box protein GRR1
- Transcriptional regulator MET32
- Mitosis inhibitor protein kinase SWE1
- Ubiquitin-conjugating enzyme E2-34 kDa
- Cell division control protein 4
- E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX5
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0019787 (ubiquitin-like protein transferase activity), GO:0004842 (ubiquitin-protein transferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0009892 (negative regulation of metabolic process), GO:0044237 (cellular metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0048523 (negative regulation of cellular process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0044248 (cellular catabolic process), GO:0019538 (protein metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0030163 (protein catabolic process), GO:0010468 (regulation of gene expression), GO:0036211 (protein modification process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0009057 (macromolecule catabolic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0043412 (macromolecule modification), GO:0044265 (cellular macromolecule catabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0051253 (negative regulation of RNA metabolic process), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0031328 (positive regulation of cellular biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0070647 (protein modification by small protein conjugation or removal), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0006355 (regulation of DNA-templated transcription), GO:0051603 (proteolysis involved in protein catabolic process), GO:1902680 (positive regulation of RNA biosynthetic process), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0010498 (proteasomal protein catabolic process), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045893 (positive regulation of DNA-templated transcription), GO:0043161 (proteasome-mediated ubiquitin-dependent protein catabolic process), GO:0045892 (negative regulation of DNA-templated transcription), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0032446 (protein modification by small protein conjugation), GO:0019941 (modification-dependent protein catabolic process), GO:0045944 (positive regulation of transcription by RNA polymerase II), GO:0006511 (ubiquitin-dependent protein catabolic process), GO:0031146 (SCF-dependent proteasomal ubiquitin-dependent protein catabolic process), GO:0016567 (protein ubiquitination), GO:0000209 (protein polyubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0140535 (intracellular protein-containing complex), GO:1990234 (transferase complex), GO:0000151 (ubiquitin ligase complex), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0031461 (cullin-RING ubiquitin ligase complex), GO:0019005 (SCF ubiquitin ligase complex), GO:0005634 (nucleus)