Protein: Protein VAPYRIN (MtVpy) (Protein HERMES) (D3J162)
Organism: Medicago truncatula (Barrel medic) (Medicago tribuloides)
Function: Required for arbuscular mycorrhizal (AM) symbiosis with AM fungi (e.g. Glomus versiforme and Gigaspora gigantea) both during fungal passage across root epidermis and for arbuscule formation in cortical cells; this symbiosis promotes phosphorus (P) and copper (Cu) uptake. Essential for infection by symbiotic nitrogen-fixing rhizobial bacteria (e.g. Sinorhizobium meliloti) leading to the formation of root nodules.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:19912567, ECO:0000269|PubMed:21223389}. Nucleus {ECO:0000269|PubMed:19912567, ECO:0000269|PubMed:21223389}. Cell membrane {ECO:0000269|PubMed:26234213}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=In cells containing arbuscular mycorrhizal (AM) fungal hyphae and arbuscules, accumulates in small puncta that move through the cytoplasm, likely mobile spherical structures that are associated with the tonoplast referred to as 'tonospheres' (PubMed:19912567). Present in cytoplasmic strands below hyphopodia (PubMed:19912567). Observed associated with EX70I in zones adjacent to the periarbuscular membrane (PAM) around the arbuscule hyphal tips (PubMed:26234213). Occasionally observed in the nucleus of cells containing fungal hyphae (PubMed:19912567). {ECO:0000269|PubMed:19912567, ECO:0000269|PubMed:26234213}.
Sequence: MDRLIKLDPSNIVLIRVEEGQKCLGKITLNNVMYTMPVAFRIQPLIKTRYTIKPQSGIISPLASLVIEITYHPPQQQGSNNLPHSFPFSDDSFLLHSVLAPGAAIKEPSSMFDSVPSDWFTTKKKQVFIDSAIKVMFVGSQILTQLVEDGNSMDDIREVLEKSDPLWESVNSKDSQGQTLLHLAISKTRPDLVQLILEFKPDIEAINSVGSTPLEAASSSGESLIVELLLAHKANTEGSESSVFRPIHHASREGHMEILRLLLLKGARVDSLTKDGNTSLHLAVEEKRRDCARLLLANGARTDVRNMREGDTPLHIAAANGDENMVKLLLHKGATKYVRNKLGKTAFDVAAENGHSRLFDALRLGDNLCAAARKGEVRTIQKVLESGGVINGRDQNGWTSLHRAAFKGRMDAVRFLVEKGIDLDAKDEDGYTALHCAAESGHADVTEFLVKKGADVEARTNKGVSALQIVESLNYVGITRILVNGGASREGLGEKPPSAPSKIPFGRKVESGSVMTMKKKMSSRTRALRGSFDHSMPLAVL
InterPro domains: - IPR036770: Ankyrin repeat-containing domain superfamily (homologous_superfamily) [131-492]
- IPR051165: Multifunctional Ankyrin Repeat Domain-Containing Protein (family) [245-488]
- IPR013783: Immunoglobulin-like fold (homologous_superfamily) [1-129]
- IPR000535: Major sperm protein (MSP) domain (domain) [4-138]
- IPR008962: PapD-like superfamily (homologous_superfamily) [2-151]
- IPR002110: Ankyrin repeat (repeat) [168-461]
Interaction partners: - Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase DMI-3
- Protein CYCLOPS
- Major sperm protein
- ABC transporter G family member STR2
- GRAS family transcription factor
- GRAS family transcription factor
- Protein NODULATION SIGNALING PATHWAY 2
- Myosin heavy chain-like protein, putative
- Ankyrin
- U-box domain-containing protein 1
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009605 (response to external stimulus), GO:0044403 (biological process involved in symbiotic interaction), GO:0051707 (response to other organism), GO:0009607 (response to biotic stimulus), GO:0042221 (response to chemical), GO:0009620 (response to fungus), GO:0036377 (arbuscular mycorrhizal association), GO:0009608 (response to symbiont), GO:0043207 (response to external biotic stimulus), GO:0009610 (response to symbiotic fungus)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)