Protein: Aminotransferase tdiD (A7XRY8)
Organism: Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
Function: Aminotransferase; part of the gene cluster that mediates the biosynthesis of terrequinone A, an antitumor agent. The first step in the biosynthetic pathway for terrequinone A is formation of indole pyruvic acid (IPA) from L-tryptophan by the aminotransferase tdiD. The nonribosomal peptide synthase tdiA then immediately converts unstable IPA to didemethylasterriquinone D (DDAQ D), via condensation of 2 IPA molecules. The symmetric connectivity of the 2 IPA molecules is thought to arise by head-to-tail dual Claisen condensations facilitated by the TE domain. TdiB then catalyzes reverse prenylation by transferring dimethylallyl diphosphate to carbon atom 2' of DDAQ D, to yield asterriquinone C-1. Finally, tdiC and tdiE enzymes robustly convert asterriquinone C-1 to terrequinone A via a transformation involving regular prenylation at carbon atom 5, which requires elimination of the hydroxy group on C-5
Sequence: MGSIGANNAVADPTPLFSSRVQKWEPGAIRSLLPLEALPGMISLVAGKPSPETFPIAEIAISLKDTPAGTGRIVVDGDELNQALQYGLPRGNAQLIQWFESLQRSVHGLDENGGWSCCIGNGSQELIHRVIQVFTDPGDPVLLETPAYPGVAGFLRADGQELIPVYSDAQGLNPASLEQALSEWPGDSPRPKVLYTTPTGSNPTGQSCTESRKAEILRLAKRFNFIILEDDAYYYLNYGDDKQRARSYLALERDVNGESGRVVRFDSLSKIVSPGMRLGILTAQAAVVDKVVRITENINLQPSSTTQLLALSLLRHWGQAGFLKHCAEAAEVYRRRRDVFVSAAERHLQGRATWVVPTAGMFVWLELKLPPEMDSFELLKSQGMKNGVLAIPGVAFMPGNEQTCYIRVSFSLVPERDMDEACRRIAGLVDRCACHS
InterPro domains: - IPR015424: Pyridoxal phosphate-dependent transferase (homologous_superfamily) [14-431]
- IPR050859: Class-I pyridoxal-phosphate-dependent aminotransferase-like (family) [9-432]
- IPR004839: Aminotransferase, class I/classII, large domain (domain) [78-425]
- IPR015421: Pyridoxal phosphate-dependent transferase, major domain (homologous_superfamily) [6-434]
Interaction partners: - monoamine oxidase
- Amine oxidase
- Probable methyltransferase tdiE
- Aromatic-amino-acid transaminase, hypothetical (Eurofung)
- aromatic-amino-acid transaminase
- Aromatic-L-amino-acid decarboxylase, putative (AFU_orthologue AFUA_3G02240)
- Indoleamine 2,3-dioxygenase
- Indoleamine 2,3-dioxygenase
- Tryptophan synthase
- tryptophan synthase
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016769 (transferase activity, transferring nitrogenous groups), GO:0008483 (transaminase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:0044249 (cellular biosynthetic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:0016053 (organic acid biosynthetic process), GO:0019752 (carboxylic acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)