Protein: Sphingosine kinase 2 (EC 2.7.1.91) (F2Y4A3)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Involved in the production of sphingolipid metabolites. Phosphorylates sphingosine and various l sphingoid long-chain base (LCB) products, such as phytosphingosine (PHS, 4-hydroxysphinganine), 4-hydroxy-8-sphingenine, 4,8-sphingadienine and D-erythro-dihydrosphingosine, but has a very few activity toward D,L-threo- dihydrosphingosine. Is required for abscisic acid (ABA) signaling that mediates stomatal closure, inhibition of seed germination and root elongation. May function upstream of PLDALPHA1 and phosphatidic acid (PA) in an amplification response to ABA that mediates stomatal closure.
Subcellular location: Vacuole membrane {ECO:0000305|PubMed:21330371}; Peripheral membrane protein {ECO:0000305|PubMed:21330371}. Note=Associated with the tonoplast.
Sequence: MENDQFMCPTDIITGIVFIDGELAMLTLTADGELRWTEYGLRQYLSMKKDVLGFIVEGKQIRVKAVVEKEAGGICCGQFGGDFVRKDFVFEPLIDQNGWCYKLRQYLDSLGRPKRLLVFVNPFGGKKSAREIFVKEVKPLFEDADVQLEIQETKYQLHAKEFVKSMDVSKYDGIVCVSGDGILVEVVNGLLERADWRNALKLPIGMVPAGTGNGMIKSLLDTVGLRCCANSATISIIRGHKRSVDVATIAQGNTKFFSVLMLAWGLIADIDIESEKFRWMGSARIDFYALQRIICLRRYNGRILFLPAPGFEGYGQPASCSLYQEPHVSDKEVGYQGPETKFEDLEWREMKGPFVTIWLHNVPWGSENTLTAPAAKFSDGYLDLIVLKNCPKLVLLSLMRQTSSGTHVESPYIVYIKVKAFVLEPGALVDEPDKEGIIDSDGEVLARGKRTYKCDQKALMSYDKLQVTVDQGLATLFSPEY
InterPro domains: - IPR050187: Lipid Phosphate Formation and Regulation (family) [97-452]
- IPR001206: Diacylglycerol kinase, catalytic domain (domain) [111-253]
- IPR017438: Inorganic polyphosphate/ATP-NAD kinase, N-terminal (homologous_superfamily) [102-244]
- IPR045540: YegS/DAGK, C-terminal domain (domain) [355-456]
- IPR016064: NAD kinase/diacylglycerol kinase-like domain superfamily (homologous_superfamily) [112-459]
Interaction partners: - Alkaline ceramidase
- Sphingosine-1-phosphate lyase
- Neutral ceramidase 1
- 3-dehydrosphinganine reductase TSC10B
- 3-dehydrosphinganine reductase TSC10A
- Sphinganine C4-monooxygenase 2
- Sphinganine C4-monooxygenase 1
- Neutral ceramidase 3
- Neutral ceramidase 2
- Sphingolipid delta
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0009845 (seed germination), GO:0009719 (response to endogenous stimulus), GO:0006807 (nitrogen compound metabolic process), GO:0051716 (cellular response to stimulus), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0009791 (post-embryonic development), GO:1901564 (organonitrogen compound metabolic process), GO:0006629 (lipid metabolic process), GO:0070887 (cellular response to chemical stimulus), GO:0044255 (cellular lipid metabolic process), GO:0071495 (cellular response to endogenous stimulus), GO:1901700 (response to oxygen-containing compound), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:0090351 (seedling development), GO:0097305 (response to alcohol), GO:0032870 (cellular response to hormone stimulus), GO:0071310 (cellular response to organic substance), GO:0033993 (response to lipid), GO:0006643 (membrane lipid metabolic process), GO:0009737 (response to abscisic acid), GO:1901701 (cellular response to oxygen-containing compound), GO:0006665 (sphingolipid metabolic process), GO:0071215 (cellular response to abscisic acid stimulus), GO:0097306 (cellular response to alcohol), GO:0071396 (cellular response to lipid)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0016020 (membrane), GO:0005737 (cytoplasm), GO:0005773 (vacuole), GO:0031090 (organelle membrane), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000325 (plant-type vacuole), GO:0043231 (intracellular membrane-bounded organelle), GO:0005774 (vacuolar membrane), GO:0098588 (bounding membrane of organelle), GO:0009705 (plant-type vacuole membrane)