Protein: Germacrene A acid 8-beta-hydroxylase (Ih8H) (IhG8H) (EC 1.14.14.168) (Cytochrome P450 71BL6) (Germacrene A acid 8-alpha-hydroxylase) (EC 1.14.14.170) (A0A2H4DGV8)
Organism: Inula hupehensis (Inula helianthus-aquatilis subsp. hupehensis)
Function: Involved in the biosynthesis of germacrene-derived sesquiterpene lactones. Hydroxylates germacrene A acid to 8-beta-hydroxy-germacrene A and 8-alpha-hydroxy-germacrene A acids. Unlike 8-alpha-hydroxy-germacrene A acid with is spontaneously converted into inunolide (12, 8-alpha), 8-beta-hydroxy-germacrene A cannot undergo spontaneous lactonization.
Subcellular location: Membrane {ECO:0000255}; Single-pass type II membrane protein {ECO:0000255}.
Sequence: MEPFTTFSLVASSLILLICWALVKANKPAKNLPPGPPKLPIIGNMHQLESQSPHRVLRKLSRKYGPIMHLQLGQVPTVVISTPRLVEEVVKHHDINFADRPTNTTSQIFYYNNQNVAWSSYGNYWRQIKKIVTLELLSVKKVRSFSSIRAEELTRAVKSVEPSVGSTINFRDLTSQTVNNMVSRATLGDVCKERHILLDTMNDILKTFNSFNVVNFFPSLQFLNVITGKKAKWLKIHKQLDHILENILEEHKSKPKGNQDDEDLIDVLLRVKDAGGQELPITNDNVKAITLEMLTAGTSSSSMTIEWAFCELMRHPEVMKKVQSDVRSAVKGNKVTEDDIQNMHYLKLVVKETLRLHGVPILVPRQNREDCNVLGYHIPAKTKILINAWACGTDPDTWEDPESFIPERFEKSSVSYFGTDFQLIPFGTGRRICPGVNFGIGTVEAVLSNFLYHFDWKLPDGVKPQDIDMTEVTGISTLPKYPLHIVPVSTVSQQK
InterPro domains: - IPR001128: Cytochrome P450 (family) [33-480]
- IPR002401: Cytochrome P450, E-class, group I (family) [61-456]
- IPR036396: Cytochrome P450 superfamily (homologous_superfamily) [25-494]
- IPR017972: Cytochrome P450, conserved site (conserved_site) [426-435]
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0004497 (monooxygenase activity), GO:0016709 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0120252 (hydrocarbon metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0006720 (isoprenoid metabolic process), GO:0042214 (terpene metabolic process), GO:0120251 (hydrocarbon biosynthetic process), GO:0008299 (isoprenoid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0051761 (sesquiterpene metabolic process), GO:0046246 (terpene biosynthetic process), GO:0051762 (sesquiterpene biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)