Protein: SH2B adapter protein 2 (Adapter protein with pleckstrin homology and Src homology 2 domains) (SH2 and PH domain-containing adapter protein APS) (Q9Z200)
Organism: Rattus norvegicus (Rat)
Function: Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways. Binds to EPOR and suppresses EPO-induced STAT5 activation, possibly through a masking effect on STAT5 docking sites in EPOR. Suppresses PDGF-induced mitogenesis (By similarity). Involved in stimulation of glucose uptake by insulin. Involved in coupling from immunoreceptor to Ras signaling. Acts as a negative regulator of cytokine signaling in collaboration with CBL. Induces cytoskeletal reorganization and neurite outgrowth in cultured neurons.
Subcellular location: Cytoplasm. Membrane. Note=Recruited to the membrane by binding to an autophosphorylated receptor after receptor stimulation by its extracellular ligand.
Sequence: MNGATPGSAAAPAPVPDWRQFCELHAQVAAVDFAHKFCRFLRDNPTYDTPDAGTSFSRHFAANFLAVFSEEVRRVLGTAADTMEPEPAVTSVTSALKTATYGHSRSSEDVSAHVATKARVRKGFSLRNMSLCVVDGVRDLWHRRASPEPEGGATPKTTEPVSEPRDKWTRRLRLARTLAAKVELVDIQREGALRFMVADDAASGPGGTAQWQKCRLLLRRAVAGERFRLEFFVPPKASRPKVSIPLSAIIEVRTTMPLEMPEKDNTFVLKVENGAEYILETIDSLQKHSWVADIQGCVDPGDSEEDTGLSCARGGCLASRVTSCSCELLTEADMPRPPETMTAVGAVVTAPHGRARDTVGESLAHVPLETFLQTLESSGGVSESNNTGDEGAELDPDAEAELELSDYPWFHGTLSRVKAAQLVLAGGPRSHGLFVIRQSETRPGECVLTFNFQGKAKHLRLSLNGHGQCHVQHLWFQSVFDMLRHFHTHPIPLESGGSADITLRSYVRAQGPPPDPGPAPNTAAPVPACWTEPAGQHYFSSLATATCPPTSPSNGAGASSSSGSSSSATSVPPRPAEGPLSARSRSNSTEHLLEAASGATEEPADATLGRARAVENQYSFY
InterPro domains: - IPR001849: Pleckstrin homology domain (domain) [186-301]
- IPR030523: SH2B adapter protein (family) [1-621]
- IPR035058: SH2B2, SH2 domain (domain) [401-496]
- IPR036860: SH2 domain superfamily (homologous_superfamily) [398-512]
- IPR015012: Phenylalanine zipper (domain) [17-73]
- IPR000980: SH2 domain (domain) [407-507]
- IPR036290: Phenylalanine zipper superfamily (homologous_superfamily) [16-75]
- IPR011993: PH-like domain superfamily (homologous_superfamily) [173-305]
Interaction partners: - E3 ubiquitin-protein ligase CBL
- Insulin receptor
- High affinity nerve growth factor receptor
- Growth factor receptor-bound protein 2
- E3 ubiquitin-protein ligase CBL-B
- BDNF/NT-3 growth factors receptor
- NT-3 growth factor receptor
- Tyrosine-protein kinase JAK2
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0060090 (molecular adaptor activity), GO:0030674 (protein-macromolecule adaptor activity), GO:0005515 (protein binding), GO:0035591 (signaling adaptor activity), GO:0005102 (signaling receptor binding), GO:0019899 (enzyme binding), GO:0019900 (kinase binding), GO:0030159 (signaling receptor complex adaptor activity), GO:0030971 (receptor tyrosine kinase binding), GO:0005068 (transmembrane receptor protein tyrosine kinase adaptor activity), GO:0019901 (protein kinase binding), GO:1990782 (protein tyrosine kinase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0032502 (developmental process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0048522 (positive regulation of cellular process), GO:0019222 (regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0048732 (gland development), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:0061008 (hepaticobiliary system development), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0010468 (regulation of gene expression), GO:0001889 (liver development), GO:1902680 (positive regulation of RNA biosynthetic process), GO:2001141 (regulation of RNA biosynthetic process), GO:0097421 (liver regeneration), GO:0006355 (regulation of DNA-templated transcription), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0045944 (positive regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0099080 (supramolecular complex), GO:0099081 (supramolecular polymer), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0099512 (supramolecular fiber), GO:0005856 (cytoskeleton), GO:0099513 (polymeric cytoskeletal fiber), GO:0005884 (actin filament), GO:0015629 (actin cytoskeleton)