Protein: Ral guanine nucleotide dissociation stimulator (RalGDS) (Ral guanine nucleotide exchange factor) (RalGEF) (Q12967)
Organism: Homo sapiens (Human)
Function: Functions as a guanine nucleotide exchange factor (GEF) activating either RalA or RalB GTPases and plays an important role in intracellular transport. Interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap (By similarity). During bacterial clearance, recognizes 'Lys-33'-linked polyubiquitinated TRAF3 and subsequently mediates assembly of the exocyst complex.
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:Q03385}. Nucleus {ECO:0000250|UniProtKB:Q03385}. Note=Localizes mainly in the peripheral region of the cytoplasmic membrane in oocytes and in preimplantation embryos until the 8-cell stage. Between the late 1-cell and the early 2-cell stages, nuclear localization becomes stronger. After the 4-cell stage, not detected in the nucleus. {ECO:0000250|UniProtKB:Q03385}.
Sequence: MVQRMWAEAAGPAGGAEPLFPGSRRSRSVWDAVRLEVGVPDSCPVVLHSFTQLDPDLPRPESSTQEIGEELINGVIYSISLRKVQLHHGGNKGQRWLGYENESALNLYETCKVRTVKAGTLEKLVEHLVPAFQGSDLSYVTIFLCTYRAFTTTQQVLDLLFKRYGRCDALTASSRYGCILPYSDEDGGPQDQLKNAISSILGTWLDQYSEDFCQPPDFPCLKQLVAYVQLNMPGSDLERRAHLLLAQLEHSEPIEAEPEALSPVPALKPTPELELALTPARAPSPVPAPAPEPEPAPTPAPGSELEVAPAPAPELQQAPEPAVGLESAPAPALELEPAPEQDPAPSQTLELEPAPAPVPSLQPSWPSPVVAENGLSEEKPHLLVFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLAPTIRATVTQFNSVANCVITTCLGNRSTKAPDRARVVEHWIEVARECRILKNFSSLYAILSALQSNSIHRLKKTWEDVSRDSFRIFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPKRAQKRPKETGIIQGTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIAPDEQFGAWFRAVERLSETESYNLSCELEPPSESASNTLRTKKNTAIVKRWSDRQAPSTELSTSGSSHSKSCDQLRCGPYLSSGDIADALSVHSAGSSSSDVEEINISFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVAATRTHKRSVSGLCNSSSALPLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQILSDDRKLKIPENANVFYAMNSTANYDFVLKKRTFTKGVKVKHGASSTLPRMKQKGLKIAKGIF
InterPro domains: - IPR036964: Ras guanine-nucleotide exchange factor, catalytic domain superfamily (homologous_superfamily) [360-649]
- IPR001895: Ras guanine-nucleotide exchange factors catalytic domain (domain) [382-649]
- IPR015758: Ral guanine nucleotide dissociation stimulator, RA domain (domain) [798-883]
- IPR019804: Ras guanine-nucleotide exchange factor, conserved site (conserved_site) [564-594]
- IPR029071: Ubiquitin-like domain superfamily (homologous_superfamily) [789-883]
- IPR000159: Ras-associating domain (domain) [798-885]
- IPR000651: Ras-like guanine nucleotide exchange factor, N-terminal (domain) [111-250]
- IPR008937: Ras-like guanine nucleotide exchange factor (family) [59-654]
- IPR023578: Ras guanine nucleotide exchange factor domain superfamily (homologous_superfamily) [105-674]
Interaction partners: - GTPase KRas
- Ras-related protein Ral-A
- Ras-related protein R-Ras
- Ras-related protein Rap-1A
- GTPase HRas
- Ras-related protein Ral-B
- Alpha-2-macroglobulin receptor-associated protein
- GTPase NRas
- Ras-related protein Rap-1b
- Ras-related protein M-Ras
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0023052 (signaling), GO:0065007 (biological regulation), GO:0051716 (cellular response to stimulus), GO:0007154 (cell communication), GO:0007165 (signal transduction), GO:0050794 (regulation of cellular process), GO:0035556 (intracellular signal transduction), GO:0007264 (small GTPase mediated signal transduction)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)