Protein: Tripartite motif-containing protein 72 (A0JPQ4)
Organism: Rattus norvegicus (Rat)
Function: Muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites (By similarity). Its ubiquitination activity is mediated by E2 ubiquitin-conjugating enzymes UBE2D1, UBE2D2 and UBE2D3 (By similarity). Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site (By similarity). This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation (By similarity). Probably acts upstream of the Ca(2+)-dependent membrane resealing process (By similarity). Required for transport of DYSF to sites of cell injury during repair patch formation (By similarity). Regulates membrane budding and exocytosis (By similarity). May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity)
Subcellular location: Cell membrane, sarcolemma. Cytoplasmic vesicle membrane. Note=Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine
Sequence: MSTAPGLLRQELSCPLCLQLFDAPVTAECGHSFCRACLIRVAGEPADDGTVACPCCQASTRPQALSTNLQLARLVEGLAQVPQGHCEEHLDPLSIYCEQDRTLVCGVCASLGSHRGHRLLPAAEAHARLKTQLPQQKAQLQEACMRKEKSVAVLEHQLVEVEETVRQFRGAVGEQLGKMRMFLAALESSLDREAERVRGEAGVALRRELSSLNSYLEQLRQMEKVLEEVADKPQTEFLMKFCLVTSRLQKILSESPPPARLDIQLPVISDDFKFQVWKKMFRALMPELEELTFDPSSAHPSLVVSASGRRVECSEQKAPPAGEDTCQFDKTVAVVAKQLLSQGEHYWEVEVGDKPRWALGVMAADASRRGRLHAVPSQGLWLLGLRDGKILEAHVEAKEPRALRTPERPPARIGLYLSFADGVLTFYDASNTDALTPLFSFHERLPGPVYPMFDVCWHDKGKNSQPLLLVGPDSEQA
InterPro domains: - IPR003879: Butyrophylin-like, SPRY domain (domain) [287-428]
- IPR001841: Zinc finger, RING-type (domain) [14-57]
- IPR013320: Concanavalin A-like lectin/glucanase domain superfamily (homologous_superfamily) [281-470]
- IPR000315: B-box-type zinc finger (domain) [81-122]
- IPR017907: Zinc finger, RING-type, conserved site (conserved_site) [29-38]
- IPR006574: SPRY-associated (domain) [288-341]
- IPR001870: B30.2/SPRY domain (domain) [271-475]
- IPR003877: SPRY domain (domain) [342-473]
- IPR043136: B30.2/SPRY domain superfamily (homologous_superfamily) [278-470]
- IPR050143: Tripartite motif-containing (family) [10-467]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [5-76]
Interaction partners: - Dysferlin
- Caveolin-3
- Caveolae-associated protein 1
- Ubiquitin-conjugating enzyme E2 H
Gene Ontology annotations:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0032501 (multicellular organismal process), GO:0042221 (response to chemical), GO:0003008 (system process), GO:0009719 (response to endogenous stimulus), GO:0003012 (muscle system process), GO:1901700 (response to oxygen-containing compound), GO:0010033 (response to organic substance), GO:0009725 (response to hormone), GO:0048545 (response to steroid hormone), GO:0097305 (response to alcohol), GO:0014070 (response to organic cyclic compound), GO:1901654 (response to ketone), GO:0033993 (response to lipid), GO:0006936 (muscle contraction), GO:0031960 (response to corticosteroid), GO:0071548 (response to dexamethasone), GO:0051384 (response to glucocorticoid)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0016020 (membrane), GO:0031410 (cytoplasmic vesicle), GO:0031090 (organelle membrane), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0031982 (vesicle), GO:0030659 (cytoplasmic vesicle membrane), GO:0012506 (vesicle membrane), GO:0043231 (intracellular membrane-bounded organelle), GO:0097708 (intracellular vesicle)