why are dideoxyribonucleoside triphosphates used during dna sequencing
When present in small amounts in sequencing reactions, dideoxyribonucleoside triphosphates (ddNTPs) terminate the sequencing reaction at different positions in the growing DNA strands.c.lack a hydroxyl (-OH) group at their 3â² end.mulsion PCR generates millions of clonally amplified sequencing template on each bead b. 400,000 DNA fragments are simultaneously sequenced c. a light signal is generated during DNA synthesis to read the sequence and the signal strength is proportional to the number of a specific nucleotide incorporated.