complex media what is agar
Growth of obligate anaerobes. The mixture of necessary nutrients can be used as a liquid medium, or a solidifying agent can be added. Agar agar is a natural polysaccharide produced by marine algae and is the most commonly used solidifying agent added to media (end concentration usually 1.5 % w/v). If hydrolysis of the agar is suspected, a silica gel is used as a replacement solidifying agent.