what temperature to incubate mouse cells
Mix well and incubate at room temperature (15 - 25Â°C) for 2.5 minutes. 5. Bring the cell suspension to a total volume of 5 mL 8 cells) or 10 mL (for 4 - 8 x 10 8 cells) by adding recommended medium.alconâ¢ 14 mL Polystyrene Round-Bottom Tubes (BD Biosciences, Catalog #352057) are recommended. 2. Add the EasySepâ¢ Mouse T Cell Isolation Cocktail at 50 ÂµL/mL of cells (e.g. for 2 mL of cells, add 100 ÂµL of cocktail). Mix well and incubate at room temperature (15 - 25Â°C) for 10 minutes.