what about your genes of interest?
The unique colony holding the gene sequence of interest is identified by comparing the main plate with bacterial colonies, the nitro cellulose membrane and the X-ray film. The identified colony carrying gene sequence of interest can then be extracted from the plate and grown as a step to amplify the gene of interest. Using expression vectors in creating cDNA library makes the screening of the cDNA library much easier which is done with the help of specific antibodies.