what methods are used to determine a viral titer
The virus titer estimates by the viable cell diameter method is close to the plaque assay-based estimates, although the cell diameter method may overestimate the titer by threefold relative to the plaque assay method. However, this is not surprising given that the two methods use different methodologies.n this method, the Vi-CELL (Beckman Coulter) was used to measure cell diameter change over a range of virus dilutions, following infection. The cell diameter change data were used to compute the virus titer using a statistical method called the method of moments that we have described previously.