how to calculate the ic50 in excel
ED50V10 (Readme) is an Excel add-in for calculating IC50/EC50 values. Input your data in the left columns, and your results will be shown in the right half of the Excel table. To calculate IC50, input 50 in the INTERPOLATE... table (highlighted in blue), the result will be shown on the right (highlighted in green).inear Regression. The simplest estimate of IC50 is to plot x-y and fit the data with a straight line (linear regression). IC50 value is then estimated using the fitted line, i.e., Y = a * X + b, IC50 = (0.5 - b)/a. Log transformation. Frequently, linear regression is not a good fit to dose-response data.