what is a mobility shift biology
A mobility shift assay is electrophoretic separation of a proteinâDNA or proteinâRNA mixture on a polyacrylamide or agarose gel for a short period (about 1.5-2 hr for a 15- to 20-cm gel).
23. In the electrophoretic mobility shift assay (EMSA), DNA-protein interactions are detected by changes in the mobility of a DNA fragment bound to a protein. A DNA fragment containing a putative protein binding site is first radiolabeled and then incubated in the presence of sequence-specific DNA binding proteins.