what is ura3 gene
Structure and function of the yeast URA3 gene: expression in Escherichia coli. Rose M, Grisafi P, Botstein D. The expression of the cloned Saccharomyces cerevisiae URA3 gene in Escherichia coli on both plasmid and phage vectors was studied.Isolates of the gene from two different laboratory strains of yeast differ in their ability to be expressed in E. coli in the absence of external adjacent promoters of transcription.The DNA sequence of the two genes was determined and revealed several differences in the DNA flanking the structural gene.One base change alters the Pribnow-box of an E. coli promoter present in the yeast sequences.solates of the gene from two different laboratory strains of yeast differ in their ability to be expressed in E. coli in the absence of external adjacent promoters of transcription. The DNA sequence of the two genes was determined and revealed several differences in the DNA flanking the structural gene.