The movement of protons through ATP synthase occurs from the
The movement of protons through ATP synthase occurs from the. matrix to the intermembrane space. matrix to the cytoplasm. intermembrane space to the matrix. intermembrane space to the cytoplasm. cytoplasm to the intermembrane space. 4.he energy released is used to move protons (hydrogen ions) across the membrane. The movement of protons increases potential energy in the form of an electrochemical gradient. Thus the chemical bond energy has been converted into potential energy.