What temperature is considered optimal for Taq polymerase activity
Taq' s optimum temperature for activity is 75â80Â°C, with a half-life of greater than 2 hours at 92.5Â°C, 40 minutes at 95Â°C and 9 minutes at 97.5Â°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72Â°C.ne of Taq' s drawbacks is its lack of 3' to 5' exonuclease proofreading activity resulting in relatively low replication fidelity.