which enzyme does hydrolysis
Enzyme hydrolysis Enzyme hydrolysis Enzymatic hydrolysis process converts pre-treated substrate to monomeric sugars, which could be further converted to ethanol or other value-added chemicals. An ideal enzymatic hydrolysis process is expected to yield maximum conversion of substrate to sugars at high consistency within reasonable time with least enzymatic input.
In addition, in living systems, most biochemical reactions, including ATP hydrolysis, take place during the catalysis of enzymes. The catalytic action of enzymes allows the hydrolysis of proteins, fats, oils, and carbohydrates. As an example, one may consider proteases, enzymes that aid digestion by hydrolyzing peptide bonds in proteins.
We investigate various enzyme and substrate related factors affecting the efficiency of hydrolysis including the effect of substrate accessibility, cellulose crystallinity, effect of hemicellulose and lignin removal, lignin-enzyme interactions, and the effect of degree of synergy between various enzyme components in the cellulase enzyme mixture.