cell surface nucleo serves as
Compacted DNA nanoparticles deliver transgenes efficiently to the lung following intrapulmonary dosing. Here we show that nucleolin, a protein known to shuttle between the nucleus, cytoplasm, and cell surface, is a receptor for DNA nanoparticles at the cell surface.By using surface plasmon resonance (SPR), we demonstrate that nucleolin binds to DNA nanoparticles directly.ince nucleolin is present at the cell surface and binds to DNA nanoparticles directly, it is a candidate receptor for the nanoparticles. Therefore, surface nucleolin expression was manipulated, and nanoparticle gene transfer observed.