is the extinction coefficient a constant
The molar extinction coefficient is a physical constant that is unique for each sequence and describes the amount of absorbance at 260 nm (A 260) of 1 mole/L DNA solution measured in 1 cm path-length cuvette.This definition is derived from the Beer-Lambert law,A = log(IO/I) = Îµ * c * p.he extinction coefficient (Îµ 260) describes the relationship between concentration and UV absorbance and can be calculated for any sequence. Greatest accuracy is therefore achieved when the exact value of Îµ 260 is calculated for each oligo.