can taq dna polymerase use dna fragments
Thermostable DNA polymerases that have a proofreading function, such as Pfu and Tli (a) Â® -T Vectors if they are first tailed with dATP using Taq DNA Polymerase (1). This A-tailing protocol can also be used to subclone DNA fragments generated by restriction enzymes which produce blunt ends (1).igure 2. Analysis of recombinants obtained after cloning of an A-tailed PCR fragment into the pGEM Â® -T Easy Vector. A 1,200bp fragment was generated by amplification with Pfu DNA polymerase. This fragment was tailed using the alternative procedure described in Figure 1B.