does transcription require dna polymerase
During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, antiparallel RNA strand called a primary transcript. Transcription proceeds in the following general steps: 1  One or more sigma factor protein binds to the RNA polymerase holoenzyme, allowing it to bind to promoter DNA.he antisense strand of DNA is read by RNA polymerase from the 3' end to the 5' end during transcription (3' â 5'). The complementary RNA is created in the opposite direction, in the 5' â 3' direction, matching the sequence of the sense strand with the exception of switching uracil for thymine.