what is the basic principle of cyanmethemoglobin determination of hemoglobin?
Methods for hemoglobinometry can be grouped into 4 main classes depending on the basic technique employed with variants within each class: 1. Colorimetric Methods. 2. Gasometric Methods. 3. Specific Gravity Methods. 4. Chemical Methods. The method of choice for hemoglobin determination is the cyanmethemoglobin method (This is a type of colorimetric method). The principle of this method is that when blood is mixed with a solution containing potassium ferricyanide and potassium cyanide, the potassium ferricyanide oxidizes iron to form methemoglobin.