what does isopropanol do for dna extraction
1 This removes co-precipitated salt and replaces the isopropanol with the more volatile ethanol, making the DNA easier to redissolve. 2  Centrifuge at 10,000â15,000 x g for 5â15 min at 4Â°C. 3  Repeat the washing and centrifugation steps once more. 4  Carefully decant the supernatant without disturbing the pellet.