where is pyruvate dehydrogenase complex located
(2). The organization of the subunits of the pyruvate dehydrogenase complex, which is located in the mitochondria in eukaryotic organisms, differs from that of gram negative organism. In gram negative organism, the complex is composed of 24 subunits of E2 that form trimers.yruvate dehydrogenase (E1) is responsible for the first two reactions. First it decarboxylates pyruvate with the help of the coenzyme TPP to form hydroxyethyl-TPP. Secondly, the hydroxyethyl group is transferred to dihydrolipoyl transacetylase (E2) through the use of lipoic acid bound to a modified lysine residue.