mechanism of of o-phenylenediamine
The apparent molar reaction enthalpy with respect to OPD and hydrogen peroxide were â447 Â± 8 kJ mol â1 and â298 Â± 9 kJ mol â1, respectively. Oxidation of OPD by H 2 O 2 catalyzed by HRP (1.25 nM) at pH 7.0 and 37 Â°C follows a pingâpong mechanism.