which enzymes are needed to produce recombinant plasmids that are used in gene transfer?
4.4.8 Outline a basic technique used for gene transfer involving plasmids, a host cell (bacterium, yeast or other cell), restriction enzymes (endonucleases) and DNA ligase. The human gene that codes for insulin can be inserted into a plasmid and then this plasmid can be inserted into a host cell such as a bacterium.