What is the purpose substrate TMB
TMB (3,3',5,5' -tetramethybezidine) is commonly used in HRP-based ELISA (emzyme-linked immunosorbent assay). This TMB reagent is formulated as all-in-one, ready to use HRP substrate.The reagent contains all essential components (TMB, substate buffer and H 2 O 2) in one solution for HRP enzyme reaction.When HRP enzyme reaction occurs, the substrate reagent produces a blue color reaction product.MB (3,3',5,5' -tetramethybezidine) is commonly used in HRP-based ELISA (emzyme-linked immunosorbent assay). This TMB reagent is formulated as all-in-one, ready to use HRP substrate.