what dictates formation of random coil
Determination of Protein Secondary Structure by Circular Dichroism: Secondary structure can be determined by CD spectroscopy in the far-UV spectral region (190-250 nm). At these wavelengths the chromophore is the peptide bond, and the signal arises when it is located in a regular, folded environment.Alpha-helix, beta-sheet, and random coil structures each give rise to a characteristic shape and magnitude of CD spectrum.This is illustrated by the graph to the right, which shows spectra for poly-lysine in these three different conformations.etermination of Protein Secondary Structure by Circular Dichroism: Secondary structure can be determined by CD spectroscopy in the far-UV spectral region (190-250 nm). At these wavelengths the chromophore is the peptide bond, and the signal arises when it is located in a regular, folded environment.