what does ammonium sulphate do in protein purification
Ammonium sulfate precipitation is a method used to purify proteins by altering their solubility.It is a specific case of a more general technique known as salting out. Ammonium sulfate is commonly used as its solubility is so high that salt solutions with high ionic strength are allowed.his is usually done by adding solid ammonium sulfate, but calculating how much ammonium sulfate to add to a solution at one concentration to achieve a desired higher concentration is tricky, since addition of ammonium sulfate significantly increases the volume of the solution.
Since proteins differ markedly in their solubilities at high ionic strength, salting-out is a very useful procedure to assist in the purification of a given protein. The commonly used salt is ammonium sulfate, as it is very water-soluble, forms two ions high in the Hofmeister series.It is generally used as a saturated aqueous solution which is diluted to the required concentration, expressed as a percentage concentration of the saturated solution (a 100% solution).his is usually done by adding solid ammonium sulfate, but calculating how much ammonium sulfate to add to a solution at one concentration to achieve a desired higher concentration is tricky, since addition of ammonium sulfate significantly increases the volume of the solution.