rna polymerase uses what as a template to synthesize what
RNA and Protein Synthesis. During transcription, the enzyme RNA polymerase synthesizes a strand of messenger RNA (mRNA) using one strand of the gene as a template.mRNA is synthesized using nucleotides that contain the bases A, C, G, and U (instead of T), which pair with the complementary bases of the DNA template strand.uring transcription, the enzyme RNA polymerase synthesizes a strand of messenger RNA (mRNA) using one strand of the gene as a template.