how to do a buffer exchange
Desalting occurs when buffer salts and other small molecules are removed from a sample in exchange for water (with the resin being pre-equilibrated in water). Buffer exchange occurs when the buffer salts in a sample are exchanged for those in another buffer.
Place column in the microcentrifuge with the mark facing outward in allsubsequent centrifugation steps.5. Add 300 ÂµL of 1x Modification buffer (pH 8.0) or appropriate buffer to the top ofthe resin bed and centrifuge at 1500 x g for 1 minute. Then discard flow-throughfrom collection tube.6.