what does pcr stand for
PCR stands for Polymerase Chain Reaction, a method for amplifying DNA in vitro. Where, in cloning DNA into a plasmid, the DNA is amplified by the bacterial cell when it replicates the plasmid, PCR amplifies DNA in a test-tube. Cycling temperatures: Of the three temperatures used in a cycle of PCR, the denaturing and extension temperatures remain unchanged for the most part, since most DNA is denatured at 94-95 degrees C, and the temperature optimum for the Taq DNA polymerase lies between 72 and 74 degrees C.
The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.n the second step, the temperature is lowered and the two DNA strands become templates for DNA polymerase to selectively amplify the target DNA. The selectivity of PCR results from the use of primers that are complementary to the DNA region targeted for amplification under specific thermal cycling conditions.