resolution phase-definition
The inevitable broadening of peaks during the chromatographic process may make it difficult to discern two consecutive peaks in a chromatogram from each other. Such a situation will occur if the peaks overlap extensively. The ability to resolve two consecutive peaks is defined as the resolution and is given by; The widths of the two closely eluting peaks are nearly equal. For quantitative work a clear separation of peaks is necessary. However it is not necessary to have a large separation between the peaks (overkill).