what is used to dissolve peptides
Neutral peptides usually dissolve in organic solvents. First, try to add a small amount of acetonitrile, methanol, or isopropanol. For very hydrophobic peptides, try to dissolve the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
Sonication can be used to enhance solubility. 1  First, assign a value of â1 to each acidic residue (Asp [D], Glu [E], and the C-terminal âCOOH). 2  If the overall charge of the peptide is positive, the peptide is basic. 3  If the overall charge of the peptide is negative, the peptide is acidic. Acidic peptides might be soluble in PBS (pH 7.4). If this fails, add a small amount of basic solvent such as 0.1 M ammonium bicarbonate to dissolve the peptide, and then add water to the desired concentration.