when a peptide bond is hydrolyzed which two functional groups are produced
One loses a hydrogen and oxygen from its carboxyl group (COOH) and the other loses a hydrogen from its amino group (NH 2). This reaction produces a molecule of water (H 2 O) and two amino acids joined by a peptide bond (-CO-NH-).The two joined amino acids are called a dipeptide. peptide bond can be broken by hydrolysis (the adding of water). In the presence of water they will break down and release 8â16 kilojoule / mol (2â4 kcal / mol) of free energy. This process is extremely slow. In living organisms, the process is catalyzed by enzymes.