differences between prokaryotes and eukaryotes dna
â¢ In eukaryotes, multiple replication sites are present in a single DNA molecule whereas, in prokaryotes, a single replication site is present in the circular DNA molecule. â¢ In prokaryotes, DNA replication involves three polymerase enzymes; namely, DNA polymerase I, DNA polymerase II, and DNA polymerase III.rokaryotic vs Eukaryotic DNA Replication. According to the Watson and Crick model suggested for DNA, one strand of DNA is the complement of the other strand; hence each strand acts as a template for the formation of a new strand of DNA. This process is known as DNA replication.